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These findings ere significant in that this is the first
laboratory proof ve hove beer, eble to generate that supports
<1
Ralph Hunch's approach to producing t'sere ''eegradable". Arcelor product for controlled usage. It is-also in agreement with the
environmental findings~to date.
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These results have been discussed with Sill Richard end Ralph Hunch. Our' concensus is that it would be worthwhile carrying out similar studies with fractionat.ef_^.r_s_c.I-Or 1130 and HCS 1016.
Additionallybased on the experience geir.ed in this first study
3 would suggest'that enough animals be included to allow sore,
incremental sacrifices after the test material had been reroved
free tbeir diet end that a lower exposure level be included.
The study would therefore be as follows - 45 animals per corpour.d
per level, 15-tc be sacrificed after 15 and 30 day recovery diets.
Ihe suggested dietary levels should.be 200 and 100 ppm and the
products studied shculd include the two previously mentioned
-
materials, fractionated Arcelor 1130, 1ICS 1016, end Aroclor 1242
as a control. It is debatable whether or not a blank control is
necevsary but if the additional cost is.not too high one shculd
probably be included.
. .. '
.
Ralph Hunch or Sill Richard shculd be contacted fer supplies of the new materials.
E. S. Tucker
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HARTOLDMON0013113
Ana lytic cl Cheuisrry Special Slue v 7 0 ~17 Job Ko. 134SC4S
DETERMIXATIOr
OF PCLTCSilCSirATE'"
EIPHEUTL RESIDUES
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"One" of the zcre sign'ficar.: object ives of the Ircclcr -- Environment program hot been to ccvtlcp a cere complete picture of whet,occurs to Arcelor products cr.cc- they are released to the ecosystem. The importance of this infcrtsiion is tvo fold, first it can be used to.guide the development of a acre "degradable" Arcelor product ar.d aecomcly, the genetal methods deve loped to study the current Arcelor - products car. be used to determine the decree of control that cost be exercised over the replacement products to avoid similar problems vith these materials ir. the future
As a pert of achieving, this overall objective* Industrial Bio-Test Laboratories css requested to carry out r 30 cay tissue collection study in albino rats vith Aroclor 1242,
This report deals with the subsequent analysis of these tissues for residual PCB's.
The details of tissue collection study can be found in the
Industrial Bio-Test report, IBT Ko. E700S. A copy of this report is
attached.
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Ta. hn e
results of 11 tissues
this study demonstrate that the residual PC3 levels decreased significantly as the degree of chlorination
and the c i'e i a~fy"~e<: pc s uf~e~~leVe l~~o f~"t he ~s s~t e r i a l~'fed decreased.....
.
Additionally, it was observed that the amount of isomeric alteration
increased as the degree of chlorination decreased resulting in the
fact that the ma jor FCB isomers retainej! Jiy_the_ rats_ fed Arcelor
1242 were those-''predominantly present -in
.......
There ver e'no"significant differences in the type of isomeric
al'fefatior. from tissue to tissue or between semes.
'
RESULTS'
The experimental data supporting the conclusions are shewn in Tables I - III, and in Figures I - VIII.
MSCUSSICH
All tissue samples were analyzed as outli red in Analytical Chemistry Method he. 70-1 and the FCB levels- c-stima tei using the Aroclor fed as the standard. Upon completion of the electron capture (EC) work all tissue extracts, for each Aroclcr fed , were combined and concentrated. The concentrates were then fu Tthsr purified via liquid chromatography on alumina and subjected t o high resolution gas
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HARTOLDMON0013114
Special Study 7 0-17 Pare 2
thrcriattifrajhy oa a support coated open tubular t-C.'O.T.) 'colucn
in an attempt 11- identify es many cf the remaining isc-ers end
hoaologa as possible-.
The EC chromacrgrams (Figures X-XII) included in this Report are
representative of those obtained for etch tissue (sv-stle, liver,
Lidney, and fat) end etch Arcelor. Tor Aroclcr .1242 the -d amine at
FCS hcmclogs retained ore pea'; numbers, (tri); 10, 13, 14 (terra);
15, 16(pcnta); 20, 22(hexa); cnc 26(he?ts). Vith
they ere, 15(penta); 20, 22{hexa); end 2d(hepta) end vith
they ere, 20, 22-(ho:/>; 26, 27 (kept*).; end 20, 25, 20(octs).
The fact that peaks 10, 13, 14, 15 end 16 ere present in Aroclor
1242 extracts but not as dominant in
.
extracts sects to indicate that 'if tha Arcelor 1242 dietary
exposure level vere reduced sufficiently these hcmclor.s veuld also
be metabolized and/or excreted es they..were vith
The very refractory homologs appear to begin vith peak 20
in the penta-hexachlorobiphenyl region.
EC chromatograms of fractionated Aroclor 1130 and KC5 1016 (Figure
IV) are include" for comparison purposes. From these, it can be
readily seen that these materials are a narked inprovement over
Aroclor 1242 in that they contain no observable amounts of the
hoDolc^s that build up.
'
The S.C.O'.T. column chromatograms (Figures VI-V1XI) of the concen
trated extracts are even tore instructive in that the greater
separation power of the colccn allows the identification of sons
of the ?CB irbmers that are not retained. For example, only
.traces of the following biphenyl isomers, dominantly present in
the material fed retain: 2-chlcrc; 3-chloro; 4-chloro; 2,6-
dichloro.; 2,4-dichioro; 2,5-dicbloro: 2,3'-dichloro; 2,4 *-dichloro;
2,5,2'-trichloro; 3,2'-dichloro; 3,4-dichlore; 2,4'-dichloro;
4,4*-dichloro; 2,3,2*-trichloro: 3,4,2'-triehioro and 2,3,4'-
trichloro. '
. ....
The S.C.O.T. column chromatograms (Figure IT) of the proposed substitutes- are again included for comparison purposes..
db `
.
Monsanto Company .
Organic Chemicals Company
Applied Sciences Section
St- Louis, Missouri .
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9/70 - V. J. Litrah.gi, B. J. Vestcnbergcr, E. S. Tucker
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Wet Wt Llpltl Wt We t Wl: 1Jp:ld Wt Wet Wt I. .ip It! wt Wet Wt Mp'<i wt i
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1094 1209 7200 7 54 4
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655 1095 3325 12972
56.9 237053.7 3353 176 5310 227 6670
M 300 2130 `3061
20.0 2056
F 300 203 5 3430
16.0 2671
M 1000
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72.5 5101 %
F 1000 11970 19080 * 91.0 7579
126 3000 125 6226 200 6 263 206' 10300
55.0 . 1396 . 36.0 1274
271 7735 16.7 6202
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Chrr-r.h::c Ccrijjtienr-
Instrument: Hevlert-Prcltcrc Mccel 402
Type Detect ;~r;
Electron Capture
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Column: 6* X 4 nr glass E, 42 SE-CO cs SO/iEC llesli Chrct*:ascrr I.*, H?
Column Ier?-:rture: 2DO1 C
Carrier*- Tje, 60 si/:
Detect::- Temperature: 2D0C It. j ertion Pert Temperature: 220eC
Purge - At/CH-, (p/1) 12C ri/uir.
Electron Capture ? c '?:
1 2 3 4 5 6 7 B 9 10 11 12 13 14 15 16 17 . 18 15 20 21 22 23 24 25 26 27 28 29 30 31
Dominant Enrclop r.s Icentifiel fry CC/MS
Axoclcr
1242
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1 1 2 2 3 3 _3
3 4 "4 44 44 44 4 55
55 5 56 6C 66 66 66 66
7 7 7 7 7 8 8 8
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30/1 ftspls inlet splitter "
Type Detector: Flame lor.itetion
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____ ^Column: ..100' X .02" FT A? S.C.0.T.,Columni.. --
. Column Temper mture: 235*C
Detector Temp erature: 2B0*C
Injection Port Temperature: 34D#C
Carrier Gas - Eeliun 6 15?
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FFA? S.C.O.T.
Column Peak Vo."
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3 ..
4
5
6
7
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9
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13
14
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19
20
21
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23-2 6
27
28-37
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Compound As Identified
by detention Tine
Bipbenyl 2 3
'4 2,6 2,2' 2,4
' 2,5 2,3* 2,4* -
' 2,5,2* 3,3*
- 3,4 * 3,4* .-
4,4*
2,3,2*
2,5,4* --*
3,4,2*
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Kunber of Chi or
0 1 1
'1 2 2 2 2 2. 2
3 -3
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2 3 2 33 3 3 3 3 3 4
3 4
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