Document e5rg8b9e6wXyR6oKoZ6N7RKrg
HAZLETON
WISCONSIN O s T O F F I C E BOX 754$ ADlSON. WISCONSIN 53707*7545
AR226-0w
a C O R N IN G uooratorv Se*v<es C o rn u a
Sponsor
3H St. Paul, Minnesota
FINAL REPORT
Study Title;
13-Week Dietary Toxicity Study with T-5180, Ammonium Perfluorooctanoate (CAS No. 3825-26-1) in Male Rats
Data Requirement: Guideline 82-1
ftuthflf: Matthew J. Palazzolo, PhD, DABT
Oi.t: May 17, 1993
Performing Laboratory
Hazleton Wisconsin, Inc. 3301 Kinsman Boulevard
Madison, Wisconsin 53704
Laboratory Pro.iect Identification:
HWI 6329-100
VOLUME I OF II
Phone
FOR E X P R E 5 S - M A U
DELIVERY
Page 1 of 657
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COMPLIANCE STATEMENT 13-Week Dietary Toxicity Study with T-5180, Ammonium
Perfluorooctanoate (CAS No. 3825-26-1) in Male Rats
All aspects of this study that were carried out by Hazleton Wisconsin, Inc., and Hazleton Washington, Inc., were in accordance with the Environmental Protection Agency Good Laboratory Practice Standards, 40 CFR 160.
Study Director Director Toxicology Hazleton Wisconsin, Inc.
To the best of my knowledge, the above statement is complete and accurate.
Sponsor's Representative 3M
Date
To the best of my knowledge, this report is complete and unaltered.
Applicant 3M
Date
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This Page Intentionally Left Blank
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QUALITY ASSURANCE STATEMENT
13-Week Dietary Toxicity Study with T-5180, Ammon1U Periluorooctanoate (CAS No. 3825-26-1) 1n Male Rats
The report for this study, with the exception of Appendix E, has been reviewed by
the Quality Assurance Unit of Hazleton Wisconsin, Inc., in accordance with the Good Laboratory Practice Standards as set forth 1n 40 CFR 160.35 (b) (6) (7). The report
Identifies and/or describes the authorized methods and Standard Operating Procedures
followed 1n the conduct of the study. The Quality Assurance Unit has also conducted the following Inspections of the testing facilities used 1n the conduct of this study and has submitted written reports of these Inspections to the Study Director.
Written status reports of Inspections and findings are submitted to Management
according to Hazleton Wisconsin, Inc., Standard Operating Procedures.
Date of Inspection
Type of Inspection
Date Issued to Studv Director
02/20/90
Protocol Review
02/20/90
03/02/90
Protocol Amendment Review
03/07/90
12/27/90
Body Weight Measurement
12/27/90
12/27/90
Food Consumption Measurement
12/27/90
12/27/90
Animal Observation
12/27/90
01/02/91
Test Material Preparation
01/02/91
01/02/91
Sample Analysis
01/02/91
01/02/91
Test Material Administration
01/02/91
01/02/91
Prepared Test Material Dispensation
01/02/91
01/14/91 01/21/91 01/24/91 02/12/91
Protocol Amendment Review Protocol Amendment Review C11nical/Pharmacok1net1c Sampling Necropsy
01/14/91 01/21/91 01/24/91 02/12/91
03/25/91 04/29/91 04/30/91
CUnical/Pharmacokinetic Sampling Oata Review Data Review
03/25/91 04/30/91 04/30/91
05/16/91
Necropsy
05/16/91
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Dito of Inspection 05/23/91
07/22-26,29-31, 08/01,02,05-07/91
09/13/91 11/18-12/04/91 11/20-12/04/91
09/28/92 02/19-24/93
03/23/93 04/08/93
QUALITY ASSURANCE STATEMENT
Type of Inspection Histology Operation Data Review
Special Analysis Data Review Report Review Protocol Amendment Review Report Review Protocol Amendment Review Report Review
HWI 6329-100
Date Issued to Study Director
05/23/91 08/07/91
09/18/91 12/05/91 12/04/91 09/28/92 02/24/93 03/23/93 04/08/93
- y ;
JohirD. Bolling
(/
Representative, Quality Assurraaince Unit
Date
3
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STUDY IDENTIFICATION
13-Week Dietary Toxicity Study with T-5180, Ammonium Perfluorooctanoate (CAS No. 3825-26-1) in Male Rats
Test Material Sponsor
Study Monitor
Study Director
Study Location
Study Timetable In-Life (Experimental) Start Date In-L1fe Termination Date Experimental Termination Date
T-5180, Ammonium Perfluorooctanoate (CAS No. 3825-26-1)
3M Toxicology Services Building 220-2E-02, 3M Center St. Paul, Minnesota 55144-1000
Roger G. Perkins, PhD, DABT 3M Toxicology Services Building 220-2E-02, 3M Center St. Paul, Minnesota 55144-1000
Matthew J. Palazzolo, PhD, DABT Hazleton Wisconsin, Inc. P.0. Box 7545 Madison, Wisconsin 53707 (608) 242-2622
Hazleton Wisconsin, Inc. 3301 Kinsman Boulevard Madison, Wisconsin 53704
December 20, 1990 May 17, 1991 May 17, 1993
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Toxicology
Matthew J. Palazzolo, PhD Dipl ornate, ABT Study Director Director
Janice D. Maneke Study Coordinator
Toxicology Operations
Larry Giltner Group Leader Sample Collection
Robert K. Young Group Leader Rodent Toxicology
Shane Eith Group Leader Support Services
Compound Preparation
Dixie Bushee Group Leader
Laboratory Animal Veterinarian
Cindy 0. Cary, DVM Diplomate, ACLAM
C l i n ^ l fjthQlpgy
Robert L. Hall, DVM, PhD Dipl ornate, ACVP Clinical Pathologist
KEY PERSONNEL
Clinical Pathology (Continued!
Ronald Markevitch Supervisor
Anatomical Pathology
Thomas E. Palmer, PhD Anatomical Pathologist
Dennis Hoffman Group Leader Necropsy
Richard Becker Group leader Histology
Anne Mosher Group Leader Pathology Data
Agricultural Chemistry
Mandla A. Tshabalala, PhD Manager Methods Development
Immunochemistrv
Joanne Satyanarayan Supervisor
Quality Assurance
Sherry R. W. Petsel Manager
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CONTENTS
VOLUME 1 OF II
COMPLIANCE STATEMENT
QUALITY ASSURANCE STATEMENT
STUDY IDENTIFICATION
KEY PERSONNEL
ABSTRACT
OBJECTIVE
REGULATORY COMPLIANCE
MAINTENANCE OF RAW DATA, RECORDS, AND-SPECIMENS
TEST MATERIAL Identification and Characterization Storage Conditions Reserve Samples Safety Precautions
TEST SYSTEM Test Animal Identification
STUDY DESIGN
PROCEDURES Dose Preparation Retention Samples Dose Analyses Dose Administration Acclimation Housing and Maintenance Antemortem Observations Body Weights Food Consumption Hormone Analysis Test Material Residue Analysis Clinical Pathology Anatomical Pathology Statistical Analyses
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Eiss
2 4
6 7 12
14 14 14 14 14 14 14 14
15 15 15 15 16 16 16 17 17 17 18 18 18 18 19 19 19 19 20
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CONTENTS (Continued)
VOLUME I OF II (Contin_uedl
RESULTS Dose Analyses Acclimation Survival Antemortem Observations Body Weights Mean Body Weight Gains Food Consumption Compound Consumption Hormone Analysis Test Material Residue Analysis Clinical Pathology Anatomical Pathology
CONCLUSIONS
SIGNATURES
REFERENCES
PATHOLOGY REPORT
COMMENTS ON THE DATA
CODES, ABBREVIATIONS, AND UNITS General Codes and Abbreviations Abbreviations and Units for Clinical Chemistry Codes for Anatomical Pathology
TABLE 1 Summary of Antemortem Observations 2 Mean Body Weights and Standard Deviations (g) 3 Mean Body Weight Gain (g) 4 Mean Food Consumption and Standard Deviations (g/wk) 5 Mean Compound Consumption (mg/kg/day) 6 Summary of Clinical Chemistry Data - Week 5 7 Summary of Clinical Chemistry Data - Week 8 8 Summary of Clinical Chemistry Data Week 14 9 Summary of Clinical Chemistry Data - Week 22
10 Summary of Absolute Organ Weight Oata (g) - 5 Week Sacrifice 11 Summary of Absolute Organ Weight Data (g) - 8 Week Sacrifice 12 Summary of Absolute Organ Weight Oata (g) - 14 Week Sacrifice 13 Summary of Absolute Organ Weight Data (g) - 22 Week Sacrifice
Page
20 21 21 21 21 22 22 22 22 23 23 23 23
24
25
26
27
31
32 33 34 36
38 41 45 49 53 56 57 58 59 60 62 64 66
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CONTENTS (Continued)
VOLUME I OF II (Continued)
Pag?
TABLE (Continued)
14 Summary of Organ-to-Body Weight Percentages - 5 Week Sacrifice 15 Summary of Organ-to-Body Weight Percentages - 8 Week Sacrifice
16 Summary of Organ-to-Body Weight Percentages - 14 Week Sacrifice
17 Summary of organ-to-Body Weight Percentages - 22 Week Sacrifice 18 Summary of Organ-to-Brain Weight Ratios - 5 Week Sacrifice 19 Summary of Organ-to-Brain Weight Ratios - 8 Week Sacrifice 20 Summary of Organ-to-Brain Weight Ratios - 14 Week Sacrifice 21 Summary of Organ-to-Brain Weight Ratios - 22 Week Sacrifice 22 Incidence of Macroscopic Observations - 5 Week Sacrifice 23 Incidence of Macroscopic Observations - 8 Week Sacrifice 24 Incidence of Macroscopic Observations - 14 Week Sacrifice
25 Incidence of Macroscopic Observations - 22 Week Sacrifice 26 Incidence of Microscopic Observations - 5 Week Sacrifice 27 Incidence of Microscopic Observations - 8 Week Sacrifice
28 Incidence of Microscopic Observations - 14 Week Sacrifice 29 Incidence of Microscopic Observations - 22 Week Sacrifice 30 Incidence of Severity of Microscopic Observations - 5 Week Sacrifice 31 Incidence of Severity of Microscopic Observations - 8 Week Sacrifice 32 Incidence of Severity of Microscopic Observations - 14 Week Sacrifice 33 Incidence of Severity of Microscopic Observations - 22 Week Sacrifice 34 Summary of Hormone Data
35 Summary of Test Material Residue Analysis
36 Results of Method Validation for Determination of T-5180 in
Rodent Food Expressed as the Percent of Recovery 37 Results of Stability Analysis
38 Results of Homogeneity Analysis 39 Results of Dose Preparation Analysis
68 70 72 74 76 78 80 82 84 87 90 93 96 99 102 105 108 111 114 117 120 122 123
126 128 130
APPENDIX A Protocol
Protocol Protocol Protocol Protocol
Material
Deviations TP9321 Amendment No. 1 Amendment No. 2 Amendment No. 3 Safety Data Sheet
132 133
134 147 150 152
154
APPENDIX B Individual Antemortem Observations Individual Body Weights (g) Individual Food Consumption (g/wk)
158 159 177
lgg
APPENDIX C Individual Clinical Chemistry Values
221 222
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CONTENTS (Continued)
VOLUME II OF II
APPENDIX D Individual
Individual Individual Individual
Absolute Organ WeightData (g) Organ-to-Body Weight Percentages 0rgan-to-Bra1n WeightRatios Animal Pathology Data
APPENDIX E Quality Assurance Statement Individual Hormone Analysis Data
APPENDIX F Results of Individual Test Material Residue Analyses
APPENDIX G Method Validation for FC-143 1n Feed Method Validation for T-5180 1n Rat Serum
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Ms
232 233 254 275 295 620 621 622 629 630 644 645 651
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ABSTRACT
The purposes of this study was to assess the subchronic toxicity of T-5180, Ammonium Perfluorooctanoate, when fed to rats for at least 13 weeks and to evaluate reversibility of any toxic effects after approximately 8 weeks without treatment. There were 320 male Crl:CD*BR rats assigned at random to six groups (55/group in Groups 1 through 5, 45/group in Group 6) and fed diets containing 0 (nonpair-fed control), 1, 10, 30, 100, or 0 ppm (control group pair-fed to the group fed 100 ppm) for up to 13 weeks. In addition, 10 animals/sex/group in Groups 1 through 5 (designated as recovery animals) were fed basal diet for 8 weeks posttreatment.
The animals were observed at least twice daily. Body weights were recorded once before initiation of treatment, weekly thereafter, and on the day of necropsy. Food consumption was recorded weekly for animals given 1, 10, or 30 ppm and for the nonpair-fed control group. Food consumption was recorded daily for the pair-fed groups (animals given 0 or 100 ppm). Food consumption was recorded weekly for all groups during recovery.
There were 15 animals/group sacrificed after 4, 7, or 13 weeks of treatment; 10 animals/group in Groups 1 through 5 were sacrificed after 13 weeks of treatment and 8 weeks without treatment. Hepatic CoA Oxidase activity was determined for five animals/group at each scheduled sacrifice.
Serum samples from 10 animals/group were collected at each scheduled sacrifice during treatment and from 5 animals/group during recovery for hormone analysis. Nonfasted rats were anesthetized with methoxyf1urane and blood was collected from the descending vena cava. These samples were analyzed for estradiol, testosterone (total) and luteinizing hormones, and for test material content.
One animal given 100 ppm was sacrificed during Week 4 due to severe neck sores; all other animals survived to scheduled sacrifice. There were no test materialrelated effects on antemortem observations. Body weight and cumulative body weight gain for the 100-ppm animals were consistently significantly lower than those of the nonpair-fed control group throughout treatment.
Animals fed 100 ppm consumed significantly less food than the nonpair-fed control group at Weeks 1 and 2. Overall, there was no significant difference in mean food consumption between the pair-fed and nonpair-fed groups.
Dietary administration of the test material resulted in higher hepatic palmitoyl CoA oxidase activity that was statistically significant at dose levels of 30, and 100 ppm at Weeks 5, 8, and 14. Animals given 10 ppm had transiently higher hepatic palmitoyl CoA oxidase activity that was statistically significant at Week 5. The mean enzyme activities for animals given 10, 30, or 100 ppm were highest at Week 8 . No difference in hepatic palmitoyl CoA oxidase activity was seen at Week 22; that is after 8 weeks recovery following the 13 weeks of dietary administration of the test material. The effect was dose-dependent and reversible.
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Administration of the test material caused increased absolute and relative liver weights and hepatocellular hypertrophy 1n the livers of animals given 10, 30, or 100 ppm after at least 4, 7, and 13 weeks of treatment. The progression of hepatocellular hypertrophy did not appear to be affected by the length of treatment. The changes observed are suggestive of a test material effect on intracellular metabolism and may be associated with peroxisome proliferation. At the end of recovery, these changes were not observed in animals, indicating that the test material-related increases in absolute and relative liver weights were reversible. There was no consistent pattern for the hormone determinations which could be attributed to the test material over the entire course of the study. None of the hormone levels were significantly different between groups although there appeared to be some indication of elevated estradiol for the 100 ppm group at the Week 5 determination. Based on the results of this study, the no-observable-adverse-effect level for T-5180 when fed ad libitum to rats for at least 13 weeks is 100 ppm; the no-effect level is 1.0 ppm.
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OBJECTIVE
The purposes of this study was to assess th subchronic toxicity of T-5180, Ammonium Perfluorooctanoate (CAS No. 3825-26-1) when fed to rats for at least 13 weeks and to evaluate reversibility of any toxic effects after approximately 8 weeks.
REGULATORY COMPLIANCE
All aspects of this study that were carried out by Hazleton Wisconsin (HWI) and Hazleton Washington (HWA) were in accordance with the Environmental Protection Agency Good Laboratory Practice Standards, 40 CFR 160.
MAINTENANCE OF RAW DATA, RECORDS, AND SPECIMENS
Original paper data, magnetically encoded records, and a copy of the final report will be retained 1n the archives of HWI. Specimens will be transferred to the Sponsor and are to be retained in accordance with 40 CFR 160.195.
TEST MATERIAL
Identification and Characterization
The test material, T-5180, Ammonium Perfluorooctanoate (CAS No. 3825-26-1), Lot No. 115, is a light colored powder. It was received at HWI on November 7, 1990, and was assigned HWI Sample No. 0058A1.
Information on synthesis methods, composition, or other characteristics that define the test material is on file with the Sponsor.
Storage Conditions The test material was stored at room temperature.
Reserve Samples
Reserve samples of the test material were taken and stored 1n a freezer. These samples were returned to the Sponsor on December 18, 1991 to be retained 1n accordance with 40 CFR 160.195. Any remaining test material was returned to the Sponsor on December 18, 1991.
Safety Precautions
Personnel involved with the study wore the apparel required by the Material Safety
Data Sheet (MSDS) provided by the Sponsor. HWI SOPs applied for any apparel not
covered by the MSDS.
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TEST SYSTEM
Test Animal
Male Crl:CDBR rats were obtained from the Portage, Michigan, facility of Charles River Laboratories, Inc. (Wilmington, Massachusetts), on December 7, 1990. The animals were approximately 41 days old at initiation of treatment and weighed 180.7 to 229.1 g.
The rat is frequently used in safety evaluation studies as a representative of a rodent species. Male rats were used to characterize the effects of the test material on testicular physiology.
Identification
Each animal was assigned a temporary number upon arrival. Before initiation of treatment, each animal was assigned a permanent number and identified with an ear tag. All data for an animal are recorded under one of these numbers.
STUDY OESIGN
Animals were assigned to the study using a computer-generated randomization according to the following design:
Group
Dose Level (pom)
Number of Males*
10
21 3 10
4 30 5 100 6 0b
55
55 55 55 55 45
a 10 animals for Groups 1 through 5 (designated as recovery
animals) were treated for at least 13 weeks, then treatment
was discontinued (animals were fed the basal diet) and they were observed for reversibility, persistence, or delayed occurrence of toxic effects for at least 8 weeks
posttreatment. b Animals in Group 6 were pair-fed with the high-dose
animals (Group 5) throughout treatment. Due to the pair feeding requirements, the pair-fed control animals (Group 6) were initiated on test one day after all other groups.
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Individual body weights at the time of randomization were within 2 standard deviations of the mean body weight and group-mean body weights were not statistically different at the 5.0% probability level (Winer, 1971).
PROCEDURES
This study was conducted in accordance with HWI Protocol TP9321 dated November 30, 1990, and Amendment Nos. 1 through 3. The protocol, protocol amendments, protocol deviations, and MSDS are in Appendix A. Unless otherwise indicated, all procedures were done in accordance with the above-referenced documents, HWI SOPs, or both.
Dose Preparation Carrier. Certified Rodent Chow* #5002 was used as supplied.
Test Material. Dietary concentrations were based on the test material as supplied. Diets were prepared weekly.
A specified amount of carrier (basal diet) was weighed into a labeled Hobart mixing bowl from which approximately 200 g were removed and placed in a Waring blender; the required amount of test material was then weighed and added to the Waring blender and overlaid with approximately 50 g of carrier, then thoroughly blended; this premix was transferred to the mixing bowl. The contents of the mixing bowl were mixed for approximately 15 minutes to prepare the base diet. Diets were made for Groups 2, 3, 4, and 5 by placing a specified amount of basal diet into a labeled Hobart mixing bowl. A specified amount of base diet was added to the mixing bowl. The contents of the mixing bowl were mixed for approximately 15 minutes.
Beginning at Week 3, the mixing procedure for Group 2 was modified. A specified amount of basal diet was placed into a labeled Hobart mixing bowl and a specified amount of the Group 3 diet was added to the mixing bowl. The contents of the mixing bowl were mixed for approximately 30 minutes. The diets were stored at room temperature (Weeks 1 through 8) or in a refrigerator (Weeks 9 through 14) in containers until diet administration.
Retention Samples Samples of each mixed batch were stored in a freezer for at least 6 months for possible future analysis.
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Dose Analyse! Assays for the test material in the basal diet were done by HWI using an analytical method developed and validated by HWI (Appendix G). Homogeneity was determined for all concentrations at Week 1. Homogeneity was also determined for the low-dose (1 ppm) concentration at Week 2. Samples taken from the top, bottom, and two opposing sides of the mix were assayed in duplicate for test material content. To evaluate the stability of the test material in the basal diet, four sets of samples were taken from each dose preparation mixed for Week 1. One set was analyzed on the day of mixing, one set was analyzed after storage for 1 day in a refrigerator and 7 days at room temperature; and the remaining sets were analyzed after being stored in a freezer for 2 or 7 weeks. A second stability was done at Week 12 to evaluate the actual storage conditions during the first eight weeks of the study. Two sets of samples were taken from each dose preparation; one set was analyzed on the day of mixing and the second set was analyzed after storage at room temperature for 8 days. All diets were assayed each week for the first 4 weeks. Each week thereafter, a sample of the control diet and one test material dietary concentration was selected sequentially for analysis. These samples were analyzed as a group approximately every 4 weeks. All samples were stored in a freezer until analyzed.
Dose Administration
Dietary admixture was used to compare data with other studies dosed in a similar fashion. The test material was administered ad libitum for at least 13 weeks. After 13 weeks of treatment, 10 males (designated as recovery animals) from all groups, except the pair-fed control group (Group 6), were fed basal diet for at least 8 weeks posttreatment.
Acclimation
The animals were received on December 7, 1990, and were acclimated in Animal Room 315 for 13 days before initiation of treatment. During acclimation, the animals were observed daily for abnormalities indicative of health problems.
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Housing and Maintenance
Animal Room 315 was used for this study. Environmental controls for the animal room were set to maintain a temperature of 72*F 6*, a relative humidity of 50% 20%, and a 12-hour 1ight/12-hour dark cycle. Variations from these conditions were documented and were considered to have had no effect on the outcome of the study.
The animals were housed individually (except for the first week of acclimation) in stainless steel, screen-bottom cages. When animal health status warranted, selected animals were individually housed in polycarbonate cages with hardwood chip bedding. Animal husbandry and housing at HWI comply with standards outlined in the Guide for the Care and Use of Laboratory Animals (NIH Publ. No. 86-23, 1985).
Certified Rodent Chow #5002 (Purina Mills, Inc.) was provided ad libitum. The lot numbers are recorded. The diet is routinely analyzed by the manufacturer for nutritional components and environmental contaminants.
Water was provided ad libitum. Samples of the water are analyzed by HWI for total dissolved solids, hardness, and specified microbiological content and for selected elements, heavy metals, organophosphates, and chlorinated hydrocarbons. The results are on file with HWI.
There were no known contaminants in the food or water that would have interfered with this study.
Antemortem Observations
The animals were observed twice daily (a.m. and p.m.) for mortality, moribundity, and signs of toxicity. At least once weekly, each animal was removed from its cage and a detailed examination was done.
Body Weights
Individual body weight data were recorded for randomization, on the first day of treatment, weekly thereafter, and on the day of necropsy (except for two animals that were not weighed before exsanguination).
Food Consumption
Individual food consumption data were recorded daily for animals that were pairfed (Groups 5 and 6) and weekly for all other animals during treatment. During recovery, food consumption was recorded weekly for all animals.
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Hormone Analysis
Serum samples were collected from 10 anlmals/group at each scheduled sacrifice during treatment and from 5 animals/group during recovery. Nonfasted rats were anesthetized with methoxyflurane, and blood was collected from the descending vena cava and stored on ice until centrifugation. Serum samples were stored at approximately -70*C (except storage location for 3 collection dates cannot be verified), then packed on dry ice, and shipped to Hazleton Washington for analysis. Serum samples were analyzed for estradiol and testosterone (total) and luteinizing hormones.
Test Material Residue Analysis
Samples of liver, testes, lungs, and subcutaneous adipose tissue were collected from all animals at necropsy and stored frozen for possible analysis. Serum samples collected from up to 10 animals/group at each scheduled sacrifice during treatment and from 5 animals/group during recovery were analyzed for test material residue.
Clinical Pathology
A section of liver was obtained from all animals at each scheduled sacrifice and flash frozen in liquid nitrogen. A section of liver from 5 animals/group at each scheduled sacrifice was assayed for the level of palmitoyl CoA oxidase as an indicator of peroxisome proliferation.
Anatomical Pathology
A necropsy was done on the animal that was sacrificed in a moribund condition.
After 4, 7, or 13 weeks of treatment, nonfasted animals were anesthetized with
methoxyflurane, weighed, exsanguinated, and necropsied between 7:00 a.m. and 10:00 a.m. (except for three animals that were necropsied after 10:00). Recovery animals were sacrificed after 8 weeks without treatment.
The necropsy included a macroscopic examination of the external surface of the
body; all orifices; the cranial cavity; the external surfaces of the brain and spinal cord; the nasal cavity and paranasal sinuses; and the thoracic, abdominal, and pelvic cavities and viscera.
At the scheduled necropsy, the following organs (when present) were weighed; paired organs were weighed separately:
Brain Liver Lungs
Testes (one testis) Accessory sex organs (seminal vesicle,
prostate, coagulating gland, urethra)
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Organ-to-body weight percentages and organ-to-brain weight ratios were calculated.
The following (when present) or representative samples were collected from scheduled sacrifice animals and preserved in 10% phosphate-buffered formalin, unless otherwise specified:
Lesions Brain Liver Lungs
Testes (one testis) Accessory sex organs (seminal vesicle,
prostate, coagulating gland, urethra)
The above (as appropriate) were embedded in paraffin, sectioned, stained with hematoxylin and eosin, and examined microscopically from all animals from which they were collected.
At the scheduled necropsy samples of the following tissues were preserved in glutaraldehyde for possible electron microscopic evaluation:
Brain Liver Lungs
Testes (one testis) Accessory sex organs (seminal vesicle,
prostate)
Statistical Analyses
The statistical methods used are described below. Levene's test (Levene, 1960) was done to test for variance homogeneity. In the case of heterogeneity of variance at p < 0.05, transformations were used to stabilize the variance.
Analysis of variance [ANOVA (Winer, 1971)] was done on the homogeneous or transformed data. If the ANOVA was significant, Games and Howell Modified TukeyKramer test (Games and Howell, 1976) was used for pairwise comparisons between groups.
One-way ANOVA was used to analyze body weights, cumulative body weight gains, food consumption, clinical chemistry, hormone values, organ weights, organ-to-body weight percentages, and organ-to-brain weight ratios. Body weights, cumulative body weight gains, and food consumption values were analyzed for all groups (except Group 6) using the SAS program according to HWI methods.
Group comparisons were evaluated at the 5.0% two-tailed probability level.
RESULTS
Groups 2, 3, 4, and 5 were compared with Group 1 ; Group 6 was compared with Group 5.
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Dose Analyses
Results of test diet assays to confirm method validation, homogeneity, and stability and to verify dose levels for T-5180 are in Tables 36 through 39.
Method validation results indicated that the method developed was suitable for the range of dietary concentration in the study.
Mean homogeneity results for diets containing 30 ppm and 100 ppm were within 10% of the theoretical levels; these results Indicated that the mixing procedure produced a homogeneous distribution of T-5180 in the diet at these levels. Mean homogeneity results for diets containing 1 ppm and 10 ppm T-5180 were within 19% and 18% of the theoretical values, respectively.
All test diets were considered stable under the storage conditions specified. The lower values of the theoretical (l.e., 78% for the sample stored refrigerated 1 day, than at room temperature 7 days, 75% for the sample stored at room temperature 8 days, and 77% for the sample stored frozen for 2 weeks before analysis) were consistent with the variability of the assay at the 1 ppm level.
Dose preparation analysis results indicated that the appropriate dietary concentrations were achieved during the study. The assay results ranged from 70.0%-119%, 90- IS-116%, 87.0%-116% and 90.1%-111% of the theoretical dose levels of 1, 10, 30, and 100 ppm.
Acclimation
For this study, 370 males were received and acclimated. In general, animals in this shipment appeared healthy. The animals were released from acclimation by the laboratory animal veterinarian on December 17, 1990. One animal was excluded from study consideration due to small testes. There were 369 males in the randomization for assignment to groups (55/group for Groups 1, 2, 3, 4, and 5; 45/group for Group 6). Animals not selected for the study were sacrificed and discarded on December 21, 1990.
Survival
One animal given 100 ppm was sacrificed during Week 4 due to severe neck sores. All other animals survived until scheduled sacrifice.
Antemortem Observations
Antemortem observations are summarized in Table 1 ; individual data are in Appendix B.
There were no test material-related antemortem observations.
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Body Weights
Body weight data are summarized in Table 2; individual data are in Appendix B.
Body weights for the 100-ppm animals were significantly lower than the pair-fed control group (Group 6) at study initiation and were consistently significantly lower than the nonpair-fed control group (Group 1) throughout treatment (Weeks 1 through 13). During recovery, there were no statistical differences in body weights noted. There were no significant body weight changes for animals at 1, 10, or 30 ppm levels.
Mean Body Weight Gains
Mean body weight gain data are summarized in Table 3.
Mean body weight gains for the 100-ppm animals were significantly higher than the pair-fed control group (Group 6) at Week 1 and were consistently significantly lower than the nonpair-fed control group (Group 1) throughout treatment (Weeks 1 through 13). Mean body weight gains were significantly lower than the nonpair-fed control group (Group 1) for animals given 10 or 30 ppm at Week 2. During recovery, there were no statistical differences in mean body weight gains noted.
Food Consumption
Food consumption data are summarized in Table 4 ; individual data are in Appendix B.
Animals fed 100 ppm (Group 5) consumed significantly less food than the nonpairfed control group (Group 1) at Weeks 1 and 2. Overall, there was no significant difference in mean food consumption between the pair-fed and nonpair-fed groups.
Compound Consumption
Compound consumption data are summarized in Table 5.
Animals were fed diets containing 1 , 10, 30, or 100 ppm of the test material. The
amount of T-5180 consumed by the animals on a mg/kg of body weight/day basis was proportional to the dose as follows:
Overall Mean SO
JL
0.06 0.013
T-5180 fmq/kq/davl
fliet Concentration (ppm)
_UL
_ 20_
0.64 0.138
1.94 0.408
100
6.50 1.214
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Hormone Analysis
Hormone analysis data are summarized in Table 34; individual data are in Appendix E. There were no statistically significant differences between groups.
There was no consistent pattern for the hormone determinations which could be attributed to the test material over the entire course of the study. None of the hormone levels were significantly different between groups although there appeared to be some indication of elevated estradiol for the 100 ppm group at the Week 5 determination.
Test Material Residue Analysis
Results of test material serum analysis are summarized in Table 35; individual data are in Appendix F. Serum levels of T-5180 increased proportionally with dietary exposure concentration but there was no increase in serum T-5180 over the course of exposure.
Clinical Pathology
Hepatic Palmitoyl CoA Oxidase data are summarized in Tables 6 through 9; individual data are in Appendix C. The Pathology Report contains a complete discussion of the data.
Dietary administration of the test material, T-5180, caused higher hepatic palmitoyl CoA oxidase activity at dose levels of 30 and 100 ppm that was statistically significant. The effect was dose-dependent and reversible. Animals given 10 ppm had transiently higher hepatic palmitoyl CoA oxidase activity that was statistically significant at Week 5 only. The mean enzyme activities for animals given 10, 30, or 100 ppm were highest at Week 8 .
Anatomical Pathology
Organ weights, organ-to-body weight percentages, and organ-to-brain weight ratios are summarized in Tables 10 through 21; macroscopic and microscopic findings are summarized in Tables 22 through 29; incidence of severity of microscopic observations are in Tables 30 through 33; all individual data are in Appendix 0. The Pathology Report contains a complete discussion of the data.
Administration of the test material, T-5180, caused increased absolute and relative liver weights and hepatocellular hypertrophy in the livers of animals after at least 4, 7, and 13 weeks of treatment. Absolute liver weights and relative liver weights for animals fed 100 ppm were significantly higher than the pair-fed controls after 4, 7, and 13 weeks of treatment. The changes observed are suggestive of a test material effect on intracellular metabolism and may be
23
0017S3
HWI 6329-100 associated with peroxisome proliferation. During recovery, these changes were not observed in animals that were treated for at-least 13 weeks and untreated for at least 8 weeks, indicating that the test material-related changes were reversible.
CONCLUSIONS Based on the results of this study, the no-observable-adverse effect level for T-5180 when fed ad libitum to rats for at least 13 weeks is 100 ppm; the no-effect level is 1.0 ppm.
24
SIGNATURES
HWI 6329-100
JarfLCe 0. Maneke S t W y Coordinator Toxicology
Matthew J. Pal . Dipl ornate, ABT
Study Director Director Toxicology
by and for Hazleton Wisconsin, Inc.
______ / n i ? Date
25
001790
HWI 6329-100
REFERENCES Games, P. A., and Howell, J. F., "Pairwise Multiple Comparison Procedures with Unequal N's and/or Variances: A Monte Carlo Study," Journal of Educational Statistics. 1(2):113-125 (1976). Institute of Laboratory Animal Resources Commission on Life Sciences, Guide for the Care and Use of Laboratory Animals. NIH Publication No. 86-23, U.S. Department of Health and Human Services, Public Health Service National Institutes of Health: Bethesda, Maryland (1985). Levene, H., "Robust Tests for Equality of Variances," Contributions to Probability and Statistics, (eds.) I. Olkin et al., Ch. 25, pp. 278-292, Stanford University Press: Stanford, California (1960). Winer, 8. J., "Design and Analysis of Single-Factor Experiments," Statistical Principles in Experimental Design. Second Ed., Ch. 3, pp. 149-260, McGraw-Hill: New York, New York (1971).
26
001794
HWI 6329-100
PATHOLOGY REPORT
SUMMARY
Dietary administration of T-5180 caused higher hepatic palmitoyl CoA oxidase activity at dose levels of 30 and 100 ppm that was statistically significant. The effect was dose-dependent and reversible. Animals given 10 ppm had transiently higher hepatic palmitoyl CoA oxidase activity that was statistically significant at Week 5. The mean enzyme activities for animals given 10, 30, or 100 ppm were highest at Week 8 .
Administration of the test material, also caused increased absolute and relative liver weights and hepatocellular hypertrophy in the livers of animals after at least 4, 7, and 13 weeks of treatment. The changes observed are suggestive of a test material effect on intracellular metabolism and may be associated with peroxisome proliferation. In the recovery phase, these changes were not observed in animals that were treated for at least 13 weeks and untreated for at least 8 weeks, indicating that the test material-related changes were reversible.
INTRODUCTION
The purposes of this study were to assess the subchronic toxicity of the test material, T-5180 (Ammonium Perfluorooctanoate), when fed to rats for at least 13 weeks and to evaluate reversibility of any toxic effects. Four groups of male Crl:CD*BR rats (55/group) were fed diets containing the test material at levels of 1, 10, 30, or 100 ppm. Two control groups received no test material; one control group was pair-fed with the high-dose group throughout treatment. Ten animals were designated as recovery animals from each group except for the pair-fed controls. After at least 13 weeks of treatment, these animals were observed for reversibility, persistence, or delayed occurrence of toxic effects for at least 8 weeks posttreatment.
During Weeks 5, 8, 14, and 22, animals were sacrificed and necropsied; macroscopic observations were recorded, organ weights were obtained, and tissues were preserved in fixatives as required by the protocol. One animal, sacrificed due to poor health during Week 4, was also necropsied. At the scheduled sacrifices, serum samples were collected for hormone analysis, tissue samples were collected and frozen for possible analysis for test material residue, and sections of liver were obtained for assaying hepatic palmitoyl CoA oxidase activity as an indicator of peroxisome proliferation. Microscopic examinations were performed on lesions, brain, liver, lungs, left testis, and accessory sex organs from all animals.
Statistical comparisons were made between the treated groups and the nonpair-fed control group and between the high-dose group and the pair-fed control group.
27
00179a
HWI 6329-100
RESULTS
Clinical Pathology
Individual values for hepatic palmitoyl CoA oxidase activity are in Appendix C. Mean values for each dose group and the results of statistical comparisons are in Tables 6 through 9.
Week 5 . Animals given 10, 30, or 100 ppm had statistically significantly higher hepatic palmitoyl CoA oxidase activity than the nonpair-fed control animals. Animals given 100 ppm also had statistically significantly higher enzyme activi ty than the pair-fed control animals. Enzyme activity increased as dose levels increased.
Week 8 . Animals given 30 or 100 ppm had statistically significantly higher hepatic palmitoyl CoA oxidase activity than the nonpair-fed control animals. Animals given 10 ppm had an apparently higher enzyme activity than the nonpair-fed control animals, but the difference was not statistically significant. Animals given 100 ppm also had statistically significantly higher enzyme activity than the pair-fed control animals. Enzyme activity increased as dose levels increased, and the activities for animals given 10, 30, or 100 ppm were higher at this interval than at Week 5.
Week 14. Animals given 30 or 100 ppm had statistically significantly higher hepatic palmitoyl CoA oxidase activity than the nonpair-fed control animals. Animals given 100 ppm also had statistically significantly higher enzyme activity than the pair-fed control animals. Enzyme activity for animals given 30 or 100 ppm were lower at this interval than at Weeks 5 or 8 .
Week 22. There were no differences for hepatic palmitoyl CoA oxidase activity between the nonpair-fed control and treated groups.
Anatomical Pathology
Individual anatomical pathology data are in Appendix 0. Individual terminal body weights, absolute organ weights, organ-to-body weight percentages, and organ-tobrain weight ratios are also in Appendix D. Mean weight values for each group are in Tables 10 through 21. Incidence summaries of the macroscopic and microscopic findings are in Tables 22 through 29.
Scheduled Sacrifices. After 4 weeks of treatment, animals given 100 ppm had significantly lower terminal body weights when compared with nonpair-fed control animals. After 4, 7, or 13 weeks of treatment, absolute and relative liver weights were significantly higher for animals given 100 ppm when compared with those of either group of control animals. These liver weights were also significantly higher in animals given 30 ppm after 4 or 7 weeks of treatment. Liver-to-body weight percentages were significantly higher in the animals given 10 ppm after 4 weeks of treatment and in the animals given 30 ppm after 13 weeks of treatment. After 13 weeks of treatment, other significant organ weight changes included lower liver-to-body weight percentages in animals given 1 ppm and higher testes-to-body weight percentages (when compared with those of the nonpair-fed controls) in animals given 100 ppm.
28
C01793
HWI 6329-100
Macroscopically, there were few observations and no major differences in incidence between control and treated animals after 4, 7, or 13 weeks of treatment.
Microscopically, minimal-to-slight hepatocellular hypertrophy was observed in the liver from animals given 10, 30, or 100 ppm after 4, 7, or 13 weeks of treatment. This change was characterized by an accentuated centrilobular pattern in which the hepatocytes appeared to have a more homogeneous cytoplasm, and the cell borders were rounded giving the cells a more plump appearance. Except for the enlarged appearance, the cells were otherwise unremarkable. The incidence and severity of this change increased with dose, but neither appeared to be affected by the length of treatment (Tables 30 through 33). All other observations were considered incidental findings.
Recovery Sacrifice. Animals given 1 ppm had significantly higher absolute brain weights. There were no other significant changes in the remaining organ weight data. Macroscopically and microscopically, there were few observations and no major differences in incidence between control and treated animals after 13 weeks of treatment and after 8 weeks without treatment.
DISCUSSION
Dietary administration of the test material caused higher hepatic palmitoyl CoA oxidase activity at dose levels of 30 or 100 ppm that was statistically significant. The effect was dose-dependent and reversible. Animals given 10 ppm had transiently higher hepatic palmitoyl CoA oxidase activity that was statistically significant at Week 5. The mean enzyme activities for animals given 10, 30, or 100 ppm were highest at Week 8. Elevation of hepatic palmitoyl CoA oxidase activity indicates that the test material causes hepatocellular peroxisome proliferation.
Dietary administration of the test material caused an increase in absolute and relative liver weights after at least 4, 7, or 13 weeks of treatment. Animals treated for at least 13 weeks and untreated for at least 8 weeks had no significant changes in liver weights. Microscopically, minimal-to-slight hepatocellular hypertrophy was observed in the livers from treated animals. These liver sections had an accentuated centrilobulated pattern in which the hepatocytes around the central vein appeared to be more plump and larger. In comparison with similar cells from the control animals, the cytoplasm of the affected cells appeared to be more homogeneous and lacking intracellular vacuoles. The cell borders were more distinct and the cells appeared to be more rounded. There was no evidence of any degenerative changes or abnormalities associated with the hypertrophy. The severity of the cellular enlargement did not appear to increase with length of treatment, and the absence of similar changes in the recovery animals 1s Indicative of reversibility. The changes observed in the liver are possibly from peroxisome proliferation or an increase in intracellular metabolism, increased storage of metabolic products, or both. All other organ weight changes and all macroscopic and microscopic observations were considered incidental findings and unrelated to ingestion of the test material.
29
C0179H
SIGNATURES
Diplomate, American College of Veterinary Pathologists
(Clinical Pathology)
T- / 7 - *73 bate
(6329-100.Ihm) 081092
HWI 6329-100
COMMENTS ON THE DATA
Various computers and computer programs were used to analyze data in this study. Because different programs round off or truncate numbers differently, values in some tables (e.g., means, standard deviations, or individual values) may differ
slightly from those in other tables, from individual calculated data, or from
statistical analysis data. Neither the integrity nor the interpretation of the data was affected by these differences.
The test material, T-5180, Ammonium Perfluorooctanoate (CAS No. 3825-26-1) is also referenced as FC-143 in the data for test material serum analysis.
The "Study Day" for the PATHTOX computer system and the in-life data collection will always be 1 day different. The PATHTOX system considers the study initiation day as "Day 1," but for all in-life data collected manually or on a data capture system (e.g., HAZTOX), the study initiation day is "Day 0."
The calculation for test material consumption is:
Test material consumption
(Dose Level x Food Consumption! 7 x [(Body Weight at Start of Week) (Body Weight Bain for Week + 2)]
31
G01796
HWI 6329-100
CODES, ABBREVIATIONS, AND UNITS General Codes and Abbreviations Abbreviations and Units for Clinical Chemistry Codes for Anatomical Pathology Note: The following lists of codes, abbreviations, and units are used by HWI. Some, but not necessarily all, of this information may be needed for this report.
32
0C1797
HWI 6329-100
N Mean SD; S.D.; STAND DEV A
B
# CV
General Codes and Abbreviations
Number of measurements in a group.
Arithmetic mean.
Standard deviation.
Group mean is significantly different from the mean of the nonpair-fed control group (Group 1) at p < 0.05.
Group mean is significantly different from the mean of the pair-fed control group (Group 6) at p 0.05.
Not applicable; No value.
Number in group.
Coefficient of Variance.
33
001798
Abbreviations and Units for Clinical Chemistry
Test
Glucose Urea nitrogen Creatinine Total protein Albumin Globulin Albumin/globulin ratio Total bilirubin Direct bilirubin Indirect bilirubin Cholesterol High-density lipoprotein cholesterol Low-density lipoprotein cholesterol Triglyceride Uric acid Aspartate aminotransferase Alanine aminotransferase Alkaline phosphatase Gamma glutamyl transferase Sorbitol dehydrogenase Bromsulphalein clearance Lactate dehydrogenase Creatine kinase Amylase Palmitoyl CoA oxidase Calcium Inorganic phosphorus Sodium Potassium Chloride Magnesium Zinc Strontium Total carbon dioxide Bicarbonate Iron Total iron binding capacity Unbound iron binding capacity Percent iron saturation Plasma cholinesterase Red blood cell cholinesterase Brain cholinesterase
Abbreviation (Units)
GLU (MG/DL) UN (MG/DL) CREAT (MG/DL) T PRO (G/DL) ALB (G/DL) GLOB (G/DL) A/G RATIO T BILI (MG/DL) D BILI (MG/DL) I BILI (MG/DL) CHOL (MG/DL) HDL (MG/DL) LDL (MG/DL) TRIG (MG/DL) UA (MG/DL) AST/SGOT (IU/L) ALT/SGPT (IU/L) ALK PHOS (IU/L) GGT (IU/L) SDH (IU/L) BSP (MG/DL) LDH (IU/L) CK (IU/L) AMYL (IU/L) PCOAO (IU/G) CA (MG/DL) I PHOS (MG/DL) NA (MMOL/L) K (MMOL/L) CL (MMOL/L) MG (MEQ/L) ZN (MG/L or PPM) SR (MG/L or PPM) TCO, (MMOL/L) BICARB (MMOL/L) FE (UG/OL) TIBC (UG/DL) UIBC (UG/DL) FE %SAT (%) CHEP (MU/ML) CHER (MU/ML) CHEB (MU/ML)
HWI 6329-100
Abbreviations and Units for Clinical Chemistry
Test
Electrophoresis Albumin Alpha-l-globulin Alpha-2-globulin Beta globulin Gamma globulin
Insulin Adrenocorticotropic hormone Cortisol Glucagon Triiodothyronine Thyroxine Electrophoresis
High-density lipoprotein Low-density lipoprotein Very-low-density lipoprotein
Abbreviation fUnitsl
E ALB (G/DL) E A-l (G/DL) E A-2 (G/DL) E BETA (G/DL) E GAMMA (G/DL) INSULIN (UU/ML) ACTH (PG/ML) CORTISOL (UG/ML) GLUCAGON (PG/ML) T3 (NG/DL) T4 (UG/DL)
E-HDL (%) E-LDL (%) E-VLDL (%)
35
CQ1SO
&&
1-2 T U M
EX NOT TAKEN MISSING UNSUITABLE AUTOLYTIC EXCLUDE
BMNIX-
n nF nO nM
HWI 6329-100
Codes for Anatomical Pathology
Definition
ANIMAL OEATH CODES
Interim sacrifices 1 and 2 Terminal sacrifice Postrecovery sacrifice #1 Sacrificed moribund
MACROSCOPIC CODES
Indicates that organ is excluded from calculations Organ weight not taken; explanation given in necropsy
notes Organ missing or lost Organ technically unsuitable for weighing Organ autolytic and could not be weighed Weight has been taken, but will be excluded from all
calculations
MICROSCOPIC CODES
Codes Prefacing Neoplastic Findings
Primary, benign neoplasm Primary, malignant neoplasm Metastatic neoplasm Locally invasive neoplasm Other neoplasm (see comment)
Distribution of Findings (n - Severity Gradel
Not specified Focal Diffuse Multifocal
36
CQISOJt
Cfldfi
1 2 3 4 5
HWI 6329-100
Codes for Anatomical Pathology
Definition
MICROSCOPIC COOES (Continued)
Grades for Severity or Amount
Minimal - the least amount of change that can be observed with the light microscope
Slight - less than average amount of change, but readily discernible as abnormal
Moderate - the average amount of change that 1s expected for a lesion
Moderately severe (marked) - a marked amount of change with possible loss of function of the affected cells/organs
Severe - a great amount of change with probable loss of function of the affected cell/organs and frequently Involves large areas of the organ
37
C01S03.
HWI 6329-100
fr-0000000f\>00
Table 1 Summary of Antemortem Observations8
Observation
Hunched posture Thin Malocclusion Hind legs, not used Crooked, tip of tail Stiff, tail Soft feces Few or no feces Rhinorrhea Epistaxis Alopecia Sores
Back Head Neck Bloody crust Back Head Left foreleg Right foreleg Neck Right forepaw Tail Abdomen Squinting Chromodacryorrhea Swollen Both forepaws Both hind paws Left hind paw Right forepaw Tail Necrotic, tail Missing Teeth Tip of tail
_o_ - L
00 00 44 00 00 00 02 00 00 00 11 9
01 01 11
02 02 01 01 33 00 00 01 00 10
00 00 00 00 00 00
01 00
T :$10 IflBSl,
_10_
30
00 00
85 00 00 00 00
00
01 11 59
10 20 11
10 21 00 00 21 01 00 00 01 01
00 00 01 00 10 00
10 00
100
1 2 6 1 1 1 1 1 0 0 13
0 0 1
0 1 1 0 3 1 1 0 0 0
1 1 0 1 0 1
0 1
0 0 0
0 0 0 0 0 0 0 0 0 1
0 0 0 0 1 0
0 0
a This table indicates the number of animals for which a condition was observed without regard to the specific nature, severity, reversibility, number of incidences per animal, or length of time the condition persisted.
38
C01503
HWI 6329-100
OOCVJOOOOOOO*- o o o
Table 1 Summary of Antemortem Observations*
Observation
Hunched posture Thin Malocclusion Hind legs, not used Crooked, tip of tail Stiff, tail Soft feces Few or no feces Rhinorrhea Epistaxis Alopecia Sores
Back Head Neck Bloody crust Back Head Left foreleg Right foreleg Neck Right forepaw Tail Abdomen Squinting Chromodacryorrhea Swollen Both forepaws Both hind paws Left hind paw Right forepaw Tail Necrotic, tail Missing Teeth Tip of tail
0 _L
00 00 44 00 00 00 02 00 00 00 11 9
01 a1 l1
02 02 01 01 33 00 00 01 00 10
00 00 00 00 00 00
01 00
T-5180 (ooml 30
00 00 85 00 00 00 00 00 01 11 59
10 20 11
10 21 00 00 21 01 00 00 01 01
00 00 01 00 10 00
10 00
100
1 2 7 1 1 1 1 1 0 0 13
0 0 1
0 1 1 0 3 1 1 0 0 0
1 1 0 1 0 1
0 1
0
0 0 0 0 0 0 0 0 0 1 0 0 0 0 1 0 0 0
a This table indicates the number of animals for which a condition was observed without regard to the specific nature, severity, reversibility, number of incidences per animal, or length of time the condition persisted.
39
GOISOV
HWI 6329-100
Table 1 (Continued) Summary of Antemortem Observations*
Observation
Small movable tissue mass Wart-like lesion
Moribund sacrifice Interim sacrifice Terminal sacrifice Recovery sacrifice
_ T-5180 (oom)
J L _1_
_1Q_
100
0
10 0 3 00
10
0
100
00 0 0 10 30 30 30 30 29 30 15 15 15 15 15 15 10 10 10 10 10 NA
a This table Indicates the number of animals for which a condition was observed without regard to the specific nature, severity, reversibility, number of incidences per animal, or length of time the condition persisted.
40 c o i s o t f ''
0180 6
GROUP AND DOSE LEVEL (PPM)
1 .000
2 1.000
3 10.000 4 30.000
Table 2 MEAN BODY WEIGHTS AND STANDARD DEVIATIONS (G)
13 WK DIETARY TOXICITY STUDY WITH T-51S0
HUI 6379-100
WEEK: START
1
2
3
4
S
6
7
8
9 10 11 12 13
MEAN S.D. N MEAN S D. N MEAN S.D. N MEAN S.D. N
204.1 8.89
55 206.4
10.60 55 202.8 11.16 55 203.7 10.19 55
262.5 11.14 55
264.2 14.13 55
260.2 IS.30 55
261.9 14.IS 55
312.9 14.12 55
313.4 17.52 55
302 8 24.50 55
303.6 23.04 55
351 6 16.77 S5
352 6 20.60 S5
347.4 21.60 55
346.3 21.70 55
366.2 20.68 55
368 5 23.42 55
382.6 24.89 55
380.3 26.64 55
MALES 418.6
22.35 46 418.7 25.93 43 416.9 27.24 44 407.6 28.72 45
437.7 26.48 40
440 1 26.11 40
438.2 27.85 40
426.0 29.27 40
457.3 28.60 40
460.9 29.56 40
458.2 29.71 40
447.6 30.75 40
475.2 31 11 25
476 9 28 78 25
479.3 3S.42 25
468.9 35.80 25
493 6 34 00 25
496.3 30 21 25
497 9 39.04 25
483.3 38.56 25
506.4 37.58 25
509.5 31 89 25
511 8 39.37 25
494.4 39.43 25
518.5 38 64 25
523.5 33.53 25
526.0 41.32 25
509.4 42.52 25
527.8 43.08 25
533.8 35.88 25
539 7 44.60 25
519.3 44.22 25
541 2 41.26 25
548.2 37 31 25
551 4 42 37 25
531 3 45 IS 25
Table 2 (Continued)
MEAN BODY WEIGHTS AND STANDARD DEVIATIONS (G) 13 WK DIETARY TOXICITY STUDY WITH T-S180
GROUP AND
DOSE LEVEL
(PPM)
WEEK: 14 IS 16 17 IS 19 20 21
1 .000
2 1.000
3 10.000 4 30.000
MEAN S.D. N MEAN S.D. N MEAN SO. N MEAN S D. N
530 8 44.39 10
545.9 42.79 10
570 5 50.64 10
531 3 41 55 10
540.4 43.23 10
556 3 44.05 10
510.5 54.24 10
545.3 42.70 10
549.7 47.61 10
566 4 49.45 10
590.2 55.72 10
556 9 43.51 10
559 1 48 .IS 10
575 4 51 62 10
599 8 57.49 10
566.3 45.10 10
559.7 46.53 10
578 5 52 99 10
597 .S 87.60
. 10 570.4
46.40 10
MALES 588.2
49.97 10 589.3 53.99 10 60S.6 69. OS 10 562.5 46.36 10
572.7 54.30 10
593 7 57.78 10
618.3 70.13 10
5S8.9 50.65 10
571.0 55.22 5
589.8 55.17 5
598.5 80.06 5
586 2 24.17 5
HWI 6379-100
08T0D
IN)
MUI 6329-100
GROUP AND DOSE LEVEL m i .....
5 100.000
6 0.000
TABLE 2 (CONTINUED)
MEAN BOOT HEIGHTS AND STANOARO DEVIATIONS (G) 13 UK DIETARY TOXICITY STUDY WITH T-5180
UEEK:
MEAN SO. N
MEAN SO. N
START
1
2
3
4
5
6
7
8
9 10 11 12 13
HALES
204.5
11.50 55
253.3* 13.61 55
295.4a 17.44 55
328.4a 22.04 55
358.4a
27.32 54
387.5a 32.04 44
410.9a
37.09 40
432.2a
39.23 40
213.1 10.31 45
255.2 8.36
45
295.3 8.54
45
334.8 10.10 45
365.2 10.81 45
396.3 13.69 30
416.7 15.84 30
434.5 19.28 30
439.1a 46.33 25
447.8 18.06 15
454.3a 49.71 25
464.3 18.39 15
461.5a 58.70 25
470.0 18.67 15
473.2* 57.67 75
482.3 19.26 15
483.3a 61.33 25
492.9 20.50 15
494.0a 64.18 25
508.5 22.27 10
^OSTGO
u
GROUP AND DOSE LEVEL (PPM)
5 100.000
6 0.000
TABLE 2 (CONTINUED)
MEAN BODY WEIGHTS AND STANDARD DEVIATIONS (G) 13 WK DIETARY TOXICITY STUDY WITH T-5I80
WEEK: 14 IS 16 17 18 19 20 21
MEAN SO. N
MEAN S.D. N
MALES
S06.2 42.93 10
516.6 43.83 10
531.6 51.67 10
536.7 48.10 10
542.0 48.84 10
550.4 51.43 10
548.9 72.78 10
523.3 77.74 5
_ _ _ - -_
-* * -*-
-
--- - -
--
HUI 6329-100
0T8TOO 01
Table 3 MEAN BODY WEIGHT GAIN (0) 13 WK DIETARY TOXICITY STUDY WITH T-5180
HWI 6329100
GROUP AND
DOSE LEVEL
(PPM)
WEEK 0-1 0-2 0-3 0-4 0-5 0-6
0-8
0-9
0-10
0-11
0-12
0-13
0-14
1 .000 2 1.000 3 10.000 4 30.000
MEAN SO.
N MEAN S D.
N MEAN S.D.
N MEAN S.D.
N
SI 3 4.59
SS 57.1
5 53 55 57.3
5.17 55 51 2
4.93 55
101 7 9.35
55 107 1
9.75 55 99.7A 21.16 55 100.0A 19.32 55
147.4 12.70 55
146.2 13.59 55
144.4 14.31 55
142 6 14.10 55
114.0 16.76 55
182.2 16.14 55
179.6 18.20 55
176.7 20.67 55
MALES
215.0 233.9 11.16 21.67 46 40
212.7 234.0
20.57 43
22.16 40
212.1 234.0 21.11 22.40 44 40
203.2 222.0 21.92 23.92 45 40
253.5 23.10 40
254.8 25.69 40
254.1 24.54 40
243.6 25.69 40
272 5 24 04 25
271 4 24.01 25
276 0 30.54 25
264.8 30.59 25
291.0 26.75 25
290.1 25.60 25
294 5 34.01 25
279.2 33.97 25
303 7 30.21 25
304.0 27.31 25
301 5 34.20 25
290.3 34.86 25
315.8 31.59 25
318.0 28.84 25
322 7 36.43 25
305.3 38.19 25
325 2 35.86 25
328.3 31.70 25
336.3 39.83 25
315.2 40.17 25
338 5 34.06 25
342.7 33.14 25
348 1 37.78 25
327.2 42.19 25
330 I 36 IS 10
341.5 41.96 10
369.1
4190.40
329.7 37 .S3 10
ui
GROUP AND
DOSE LEVEL
(PPM!
WEEK
0-15
0-16
Table 3 (Continued) MEAN BODY WEIGHT GAIN |Q1 13 WK DIETARY TOXICITY STUDY WITH T-5110
0-17 0-1 0-1 0-20 0-21
1 .000 2 1.000 3 10.000 4 30.000
MEAN S.D.
N MEAN S.D.
N MEAN S.D.
N MEAN S.D.
N
340. 4 36 .3 10
351 .9 42 .15 10
37. 1 S3. 30 10
336 7 31. 96 10
349 . 1
41 .01 10 362 .1 47 .90 10 316 .7 55 .43 10 34 4 39 21 10
359 1 40 01 10
371 0 50 01 10
391 .4 57 SO 10
3S7 .8
41. 10 10
359 7 40 .06 10
374 .2 51 .42 10
396. 4 61 12 10
361 6
42. 21 10
MALES 36. 2 372.7
43 19 41.33 10 10 365 .0 369.4 S3 09 57.21 10 10 407 2 416. 69 .24 70.33 10 10 374 0 360.3 42. 61 46.56 10 10
376.. 6
SO. 30 5
364 . 8
53 .06 5
393..3 77 .07 5
376 .3 2. 25 5
HWI 6329100
fTSTOO
9
INI 6329 100
GROUP AND OOSE LEVEL iff?).....
5 100.000
6 0 000
TABLE 3 ( c o m m u t o )
MEAN BOOT WEIGHT GAIN (G) 13 UK DIETARY TOXICITY STUDY WITH 1-5180
WEEK:
HEAN S0 N MEAN S0 N
0-1 0-2 0-3 0 4 0-5 0-6
0-7 0-8
0-9
0-10
0-11
0-12
0-13
0-14
; oB
IS os ss
42 0 5 16
4S
89.0* 18.31 55
84.4 17.34 45
123.9* 17.41 55
121.7 8.73
45
153 8* 23 62 54
152.0 8 58
45
HALES
182.7*
26 43 44
206.2* 30.04 40
227.6*
32.96 40
234 9*
38.10 25
182.1 10.73 45
202.5 12.79 30
219.6 16.55 30
235 2 16.45 15
250 0* 41.43 25
251.8 16 06 15
257 2* 50 62 25
257.4 16.60 15
269.0* 49 96 25
269 8 17.07 15
279 0* 53.93 25
273 ? 24.92 15
289.7*
57 40 25
303.4
43.09 10
295.9 17.76
10
.
-
c o l s i A,
--I
GROUP AND DOSE LEVEL (PPM)
5 100.000
TABLE 3 (CONTINUED)
MEAN BODY WEIGHT GAIN (G) 13 UK DIETARY TOXICITY STUDY WITH T-5180
WEEK.
0-15
0-16
0-17
0-18
0-19
0-70
0-71
MEAN
SO. N
313 8 43.94 10
378.8 57.73 to
331.6 48.78 10
336.9 48.94 10
345 3 51.39 10
MALES
343.8 73.38 10
313.0 78.57 5
IMI 6329-100
T8TG0
oo
GROUP ANO DOSE LEVEL (PPM)
WEEK:
1
Table 4 MEAN FOOD CONSUMPTION AND STANDARD DEVIATIONS (G/WK)
13 WK DIETARY TOXICITY STUDY WITH T-S1IO
2 3 4 5 6 7 8 9 10 11 12 13 14
1 .000
2 1.000
3 10.000 4 30.000
MEAN S.D. N MEAN S.D. N MEAN S.D. N MEAN S.D. N
161. 10.55 54
167.2 10.95 54
166.5 12.11 53
168.6 10.67 54
160.7 13.04 S3
178.3 13.44 55
179.2 19.78 53
183.3 24.70 54
166.1 18.95 53
188.2 14.45 52
183.8 17.25 50
178.7 33.72 52
193.8 14.36 51
196.0 15.10 52
191.0 16.74 49
190.2 17.5 52
196.5 14.32 43
201.4 14.87 38
199.6 14.82 40
203.0 20.77 39
MALES 195.3
14.78 35 197.0 14.30 33 194.4 14.63 37 193. 18.11 37
192.9 14.83 39
195.4 15.81 36
194.4 14.39 39
193.0 15.69 37
188.5 17.99 24
191.5 13.13 24
189.0 14.22 23
188.8 15.53 23
193.8 16 43 24
196.8 12.20 23
193.1 16.41 24
191.9 15.81 24
189.5 16.18 24
190.4 12.41 24
190.9 15.21 23
187.3 14.15 22
184.7 20.08 23 190.2 15.77 23
189.2 15.64 24
188.9 14.99 24
183.4 20.64 22 197.2 15.73 23 168.1 15.55 22
185.6 16.73 24
180.6 14.86 20
180.5 19.17 22
181.9 16.61 23
176.6 16.68 23
175.8 16.49 6
172.2 19.08 10
191.6 17.75 9
174.6 10.39 9
o o
00
GROUP AND DOSE LEVEL (PPMI
WEEK.
15
Table 4 (Continued) MEAN FOOO CONSUMPTION AND STANDARD DEVIATIONS |G/WK)
13 WK DIETARY TOXICITY STUDY WITH T-5180
1 17 18 19 20 21
1 .000
2 1.000
3 10.000 4 30.000
MEAN SO. N MEAN S.D. N MEAN SO N MEAN S.D. N
180.2 11.56 10
178.7 19.60 10
185.9 17 16 10
177.1 9.35 10
174 8 13.67 10
177.3 17.29 10
183.6 15 82 10
181.2 12.37 9
179.2 9.SO
10 176.1
21.40 10 183.9 16.46 10 183.8 9.35 9
191.4 15.78 9
191 1 17 28 10
186 3 30.04 9
189.1 8.55 9
182.4 20.45 10
187.2 19.12 10
199.1 21.95 10
193.9 8.32 9
MALES 161.8
19.48 10 182.8 20 56 10 189.6 19.12 10 169.1
9.92 9
190 5 11.90 5
198.0 25.32 5
189.1 19.69 5
192.8 11.86 5
ton
GROUP AND DOSt U V U (PPM)
S 100 000
6 0 000
TABLE 4 (CONTINUED)
MEAN FOOD CONSUMPTION (G/WK) 13 WK DIETART TOXICITY STUOT WITH T-5180
HUI 6379-100
WEEK:
1
7
34
56
78
9 10 II 1? 13
MEAN SD N
MEAN SD N
155 0* 9 54
55
153.8 7 13
45
165.1* II 36 55
164.8 0 74
44
179.3 15 59 55
178.0 4 57
43
185.3 15.76 54
183 0 4.73
43
193.1 17.76 47
187.9 10.17 73
MAIES
190 3
17 91 39
187.7
17.56 37
183.5 II 00 75
178.6 17.30 30
1818 18.48 79
176.3 9.11 14
185 7 19 78 75
179 5 8 43 15
176.5 76.75 74
171 4 6.05 17
17? 9 76.56 74
170 0 4.74 14
177 7 73.40 75
171.5 7.65
15
171.7 76 04 75
166.8 5 86 8
O O
P
Oh in
p
SN
GROUP AND OOSE LEVEL (PPH)
5 100.000
TABLE 4 (CONTINUED)
MEAN FOOD CONSUMPTION (G/UK) 13 UK DIETARY TOXICITY STUDY WITH T-5180
WEEK:
14
IS
16
17
18
19
20
21
MEAN
SO N
164.8 22.52 10
178.8 19.02 10
176.0 22.12 10
172.6 18.40 10
178.8 17.16 10
MALES
177.6 16.15 10
164.5
41.88 10
174.9 15.95 10
HU I 6329-100
001S1
uii
GROUP AND DOSE LEVEL (PPM)
WEEK:
1 .000
2 1.000 3 10.000 4 30.000
MEAN SD N MEAN SO. N MEAN SO. N MEAN S.D. N
Table 5 MEAN COMPOUND CONSUMPTION (MG/KG/DAY | 13 WK DIETARY TOXICITY STUDY WITH T-5180
HWI 6329-100
1
00 .00 54 .09 00 54 .91 .04 53 2 75 .10 54
2
00 .00 53 .01 .00 55 .15 .13 53 2.59 .33 54
34
.00 00 53 01 00 52 .75 .05 50 2 20 .39 52
.00 .00 51 .07 .00 52 .71 .03 49 2.14 .15 52
5
MALES .00 00
43 .07 .00
39 .61 .03
40 2.13 IS
39
6
.00 .00 35 .06 .00 33 .63 .03 37 1.96 .13 37
7
.00 00 38 06 00 36 60 .02 39 1 85 08 37
8
.00 00 24 06 00 24 56 02 23 1 72 .07 23
9
.00 00 24 06 00 23 .56 .03 24 1.71 .08 24
10
.00 00 24 .05 00 24 53 02 23 161 07 22
11
00 00 23 05 00 23 51 02 24 1 $8 07 24
12
00 00 22 05 00 23 50 02 22 1 52 08 24
#TSTGD
cn
Ui
coisf^
GROUP AND DOSE LEVEL (PPM|
WEEK:
13
1 000
2 1.000 3 10 000 4 30.000
MEAN SD N MEAN SD N MEAN SO N MEAN S D. N
.00 00 20 .05 .00 22 .47 03 23 1.44 10 23
Table 5 (Continued) MEAN COMPOUND CONSUMPTION (MG/KG/DAY) 13 WK DIETARY TOXICITY STUDY WITH T-51D0
HVI 6329-100
INI 6329-100
TABLE 5 (CONTINUED)
MEAN COMPOUND CONSUMPTION (MG/KG/DAV) 13 UK DIETARY TOXICITY STUDY WITH T-5180
i
GROUP ANO OOSE LEVEL (PPM)
WEEK:
17
3 4 56 78
9 10 II 12 13
5
100.000
6 0.000
MEAN SO. N
MEAN SO. N
6.75 0.34 55
.00 .00 45
7.99 0.35 55
.00 .00 45
7.80 0.43 55
.00 .00 45
7.39 0.40 54
.00 .00 45
7.13 0.38 42
.00 .00 30
HALES
6.64 0.36 39
6.21 0.32 37
.00 .00 30
.00 .00 30
5.88 0.35 25
.00 .00 15
5.84 0.39 25
.00 .00 15
5.46 0.47 24
.00 .00 IS
5.23 0.48 24
.00 .00 15
5.25 0.41 25
.00 .00 15
4.97 0.43 25
.00 .00 9
o
00->
K) cinn
t"
i
6 UO)1
09
14
DOSE PPM
Table 6
SUMMARY OF CLINICAL CHEMISTRY DATA
MALES
WEEK S
PCOAO IU/Q
0 MEAN SO. N
1 MEAN S.D. N
10 MEAN S.D. N
30 MEAN S.D. N
100 MEAN S.D. N
0 MEAN S.D. N
1 S
5
9 17 5
A 14
3.6 S 24 A 11.4 5
AB 37 14.6
5
.4
5
HWI 632B100
2P28T00
i
cn --l
DOSE PPM
Table 7
SUMMARY OF CLINICAL CHEMISTRY DATA
MALES
WEEK I
PCOAO IU/Q
(AN SO. N (AN SO. N MEAN SO. N MEAN S.D. N MEAN S.D. N MEAN S.D. N
7 15 5 71 5 16 5.5 5 32 A 12.2 5
AB 54 35.3
5 7 1.5 5
HWI 6320100
J^BTGO
DOSE PPM
Table 8
SUMUftY OF CLINICAL CHEMISTRY DATA
MALES
WEEK 14
PCOAO IU/Q
0 MEAN SO. N
1 MAN SO. N
10 MEAN SO. N
30 MEAN SO. N
100 MEAN SO. N
0 MEAN SO. N
6 .0
s 1.0 5 10 2.1 5 14* 3.4 S
AB 17
4.5 S 5 1.1 5
uC Dt
HWI 6320100
rzsToo
DOSE PPM
Table 9
SUMMARY OF CLINICAL CHEMISTRY DATA
MALES
WEEK 22
PCOAO IU/Q
0 MEAN SD N
1 MEAN SO. N
10 AN S D. N
30 MEAN SO. N
100
MEAN SD N
7 7
5 7 1 5 7 11 S
71. 1
S 7
.4 5
i\on
HWI 6329100
HAZLETON LABORATORIES AMERICA, INC. MADISON. WISCONSIN U S A .
TABLE 10 SUMMARY OF ABSOLUTE ORGAN WEIGHT DATA (G|
S WEEK SACRIFICE
TABLE INCLUDES: SEX-ALL;GROUP-ALL;WEEKS-ALL DEATH-1;SUBSET-ALL
SEX GROUP NUMBER
-- 1-- IS
.......MALE 15 IS 15
14
IN GRP. MEAN STAND DEV
15 411 1
21.7
>>> TERMINAL BODY WEIGHT 19) <<<
15 15 15 14*
413.9
398.4
403.3
378.4
34.5
32.0
35.5
18.4
IN GRP. MEAN STAND DEV
IS 2.0732
.0812
>>> BR - BRAIN <<<
15 15 15
2.0275 2.0452 2.0604
.0842
.0966
.0759
14 2.0437
.0996
IN GRP. MEAN STAND DEV
IS 16.3401
2.1378
>>> LI - LIVER <<< 15 15 15A IS 4495 17 8942 23.2303 1.7139 2.1294 2.8382
14 A B 25.4377
1.9900
IN GRP. MEAN STAND DEV
15 1 5138
.2006
>>> LU - LUNG <<<
15 15
1.5312
1.4426
.0989
.1122
15 1.4932
.1231
14 1.4140
.0828
IN GRP. MEAN STAND DEV
15 1.6442
.1250
>>> TEO - LF. TESTIS <<<
15 IS 15
1 6222
1 6454
1.5874
.1410
.1241
.0916
14 1.5794
.1704
15 15 388.9 11.1 15 2.0172
.0857 15 15.8344 1.1305 15 1.5376
.1266 15 1.6102
.1234
r> Q
GO o
HWI 6329-100 PRINTED. 1S-OCT-91 TABLE.PAGE: 10.1
^,28T00
HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U S A .
10TABLE
SUMMARY OF ABSOLUTE ORGAN WEIGHT DATA (Q|
5 WEEK SACRIFICE
TABLE INCLUDES: SEX.ALL;GROUP-ALL;WEEKS-ALL DEATH*!;SU8SET*ALL
I
11 It
i1
1 *|
11
SEX: GROUP: NUMBER:
-- 1 --IS
15
..... MALE--3........
IS
A
IN GRP.. MEAN: STAND DEV:
IS 1.8523
.1273
>>> TE1 - RT. TESTIS <<<
15 IS 15
1.8169
1.6520
1.5904
.1243
.1059
.1095
IN G R P .: MEAN.
STAND DEV.
15 2.6101
2257
>>> SO - ACCESS/SEX ORG <<<
15 15 15
2.6799 2.9350 2.6973
.3592
.4096
.3136
14 1.6260
.1013 14 2.6319
.2549
1 1 1 11 <1 1 1*> 1 1 4 11 11 1
15 15 1.6001
.1212 15 2.4999
.3579
HW1 6329-100 PRINTED: 15-OCT-91 TABLE PAGE: 10 2
HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN US.A.
TABLE 11 SUMMARY OF ABSOLUTE ORGAN WEIGHT DATA (G|
1 WEEK SACRIFICE
TABLE INCLUDES: SEX-ALL;GROUP-ALL;WEEKS-ALL DEATH-2;SUBSET-ALL
SEX GROUP NUMBER
IS
.......MALE 15 15 15
15
IN GRP MEAN STAND DEV
IS 473.1
26.0
>> IS
TERMINAL BODY WEIGHT 15 15
(9)
1<5<<
414.0
470.9
466.9
459.0
36.6
21.6
21.7
34.2
IN GRP. MEAN STAND DEV
IS 2.0923
.0896
>>> 6R - BRAIN <<<
IS 15 15
2.0739 2.0152 2.1032
.0649
0>S1
.0650
15 2.0S5
.010
IN GRP. MEAN STAND DEV
15 17.7610
2.1244
>>> LI - LIVER <<< IS IS 15 A 17 679 19.4195 24.0114 2.7374 2.0972 3.579
15 A B 27 7557
3.5077
IN GRP. MEAN STAND DEV
IS 1.5*29
.1077
>> LU - LUNG <<<
15 15
1.617
1 5339
.1110
.1297
15 1.4902
.1217
15 1.5201
.1904
IN GRP. MEAN STANO DEV
15 1 6101
.1110
>>> TEO - IF. TESTIS <<<
IS 15 15
1 6166
1.6664
1.7163
.0121
.2296
.1649
15 1.6115
.1265
-- 6 --15 15
449.3 24.9 IS 2.0162 .0907 15 16.907 2.2232 15 1.5615 .1317 15 1.6305 .1177
r>
05
HWI 6329-100 PRINTED: 15-OCT-91 TABLE.PAGE: 11.1
HAZLETON LABORATORIES AMERICA, INC. MADISON. WISCONSIN U.S.A.
TABLE 11 SUMMARY OF ABSOLUTE ORGAN WEIGHT DATA (Gl
I WEEK SACRIFICE
TABLE INCLUDES: SEX-ALL:GROUP-ALL:WEEKS-ALL DEATH-2;SUBSET-ALL
SEX GROUP: NUMBER:
15
---2--15
..... MALE--3........ -4---
IS 15
---5 -- 15
IN GRP.: MEAN: STAND DEV:
15 1.7036
.1052
>>> TEI - RT. TESTIS <<<
IS 15 15
1.6675
1.6904
1.7911
.1076
.1397
.2401
15 1.6533
.1070
IN GRP.: MEAN: STAND DEV:
15 3.0673
.3617
>>> SO - ACCESS/SEX ORG <<<
15 IS IS IS
3.0990 3.4446 3.1306 2.6513
.4021
.3649
.3164
.2775
15 14 1.6335
.1111 IS 2.6606
.4391
HWI 6329-100 PRINTED: 15-OCT-91 TABLE.PAGE: 11.2
r>
Ol
p00
M
HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U S A .
TABLE 12 SUMMARY OF ABSOLUTE ORGAN WEIOHT DATA (G)
14 WEEK SACRIFICE
TABLE INCLUDES. SEX-ALL GROUP.ALL ;WEEKS-ALL DEATH-T;SUBSET-ALL
SEX .......MALE
GROUP
NUMBER
IS
15
15
15
IS
is
1 IN GRP MEAN STAND DEV
15 55. 7
41.1
>>> TERMINAL BODY WEIGHT 14 15 15
(9)
1<4<<
555.1
552.7
53. 2
489.1
42.0
40.7
58.1
79.0
15 503.5
23.7
IN GRP. MEAN STAND DEV
15 2.1914
.1183
>>> BR - BRAIN <<<
14 15 15
2.2279 2.1740 2.1011
.2745
.0196
.0724
14 2.1342
.1044
15 2.1364
.0594
IN GRP. MEAN STANO DEV
15 19 7299 2 2290
>>> LI - LIVER <<< 14 15 15 IS.0355 20 4416 22.7367 2 1120 2 1657 4.2121
14 A B 26.7846
5.4675
15 16.3033
1.6241
IN GRP. MEAN STAND DEV
8 IN GRP. MEAN STAND DEV
15 1 6543
1403 15 1 7591
.1174
>>> LU - LUNG <<<
14 15
1.6001
1.5803
.1166
.2250
15 1.6101
.1316
>>> TEO - LF. TESTIS <<<
14 15 15
1 7329
1.7767
1.7771
.2279
.1017
.1216
14 1 5123
.1401
15 1.5875
.1018
14 1.7576
.1547
15 1 7117
.1381
HWI 6329-100 PRINTED: ll-OCT-91 TABLE.PAGE: 1 2 1
HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U S A .
TABLE 12 SUMMARY OF ABSOLUTE ORGAN WEIGHT DATA (G|
14 WEEK SACRIFICE
TABLE INCLUDES: SEXALL.GROUPALL.WEEKS-ALL OEATHT;SUBSET-ALL
SEX GROUP
NUMBER
IS
....... M A L E - - 3 .........
-4-..
--5---
IS IS 15 15
IN G R P .
MEAN STAND DEV
IS
1.7572 .112
>>> TE1 14 1.9269
.2499
- RT. T E ST IS <<<
15 15
1.7519
1.7759
.0772
.1197
14
1.7373 .1460
IN GRP. MEAN
STAND DEV
15 3.5217
.4974
>>> S O - AC CE S S / S E X O R G <<<
14 15 15
3.4797
3.5435
3.3760
.429
.5535
.4792
14 3.3647
.6309
---6 -- 15
15 1.6903
.1204
15 3.2597
.6635
HWI 6329-100 PRINTED: ll-OCT-91 TABLE.PAGE: 12.2
coisap
A<nn
0183
HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U S A .
TABLE 13 SUMMARY OF ABSOLUTE ORGAN WEIGHT DATA |G)
22 WEEK SACRIFICE
TABLE INCLUDES: SEX*ALL:GROUP*1.2,3,4,5,WEEKS-ALL
0EATH*U;SUBSE TALL
G NU
RMOBSEUEXPR:::
---1--10
--2--10
--MALE------
---3--- ---4--10 10
10
IN GRP.. MEAN: STAND DEV:
10 57 1
61.6
> > > TERMINAL BOOY WEIGHT ( g ) < < <
10 10 10 10
607 1 SI 1
129.2 73.4
601.1 51.2
55696..0a
IN ORP.:
MEAN: STANO DEV.
>>> BR - BRAIN <<<
10 10 A 10
2.1321 2 2465 2.2042
.0622
.0496
.0774
10 2.1122
.0163
10 2.1345
.0909
M
IN E
GRP AN
.
::
STAND DEV:
> > > LI - LIVER < < <
10 10 10 IS.6432 It 4373 11.9203
3.0(67 1.7410 2.2968
10 20 7334
1.1916
10 11.6190
2.4465
IN GRP.:
MEAN: STAND DEV:
> > > LU - LUNG < < <
10 10 10
1.5961 1 7415 1.6100
.1050
1S4S
.1127
10 1.6937
.1476
10 1.5919
.1130
aM IEN AGRNP.: :
STAND DEV
>>> TEO - LF . TESTIS c < <
10 10 10 10
1.6934
1.7559
1.739
1 7055
.1536
.1607
.2194
.2248
10 1.7590
.1509
<n
o
HWI 6329-100 PRINTED: ll-OCT-91 TABLE PAGE: 1 3 1
HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U S A .
TABLE 13 SUMMARY OF ABSOLUTE ORGAN WEIGHT DATA (G)
22 WEEK SACRIFICE
TABLE INCLUOES: SEX*ALL;GROUP1.2,3.4.5;WEEKS-ALL DA7H*U;SUBSET-ALL
SEX: GROUP:
-- 1---
NUMBER.
10
---2--10
-MALE----3---
10 10
IN GRP : MEAN: STANO DEV:
>>> TE1 - RT. TESTIS <<<
10 10 10 10
1 7544 ' 1.7592
1.7991
1.7913
1811
.1739
.1134
.1655
IN GRP.: MEAN STAND DEV:
>>> SO - ACCESS/SEX ORG <<<
10 10 10 10
3 5540 3 6512 3.5191 3.9577
5410
.5401
.2931
.4056
10
1.7355 .1118
10 3.6939
.6923
HWI 6329-100 PRINTED: ll-OCT-91 TABLE PAGE 13 2
tfDSTGO
O*~i4
HAZLETON LABORATORIES AMERICA. INC. MADISON, WISCONSIN U S A .
TABLE 14 SUMMARY OF ORGAN-TO-BOOY WEIGHT PERCENTAGES
5 WEEK SACRIFICE
TABLE INCLUDES: SEX-ALL :GROUP-ALL;WEEKS-ALL DEATH-1 :SUBSET-ALL
SEX GROUP NUMBER
IS
..... MALE IS 15 15
IN GRP. MEAN
STAND DEV
IS .5053 .0260
>>> BR - BRAIN <<<
15 IS 15
.4927
.5162
.5142
.0416
.0474
.0444
IN GRP. MEAN STAND DEV
15 3.9690
.3714
>>> LI - LIVER <<<
15 ISA 15 A
3.7297 4.4146 5.7612
.2293
.3162
.6020
9 IN GRP. MEAN STAND DEV
15 .3676 .0413
>>> LU - LUNG <<<
15 15
.3710
.3625
.0216
.0162
15 .3713 .0271
IN ORP. MEAN STAND DEV
15 .4007 .0332
>>> TEO - LF. TESTIS <<<
15 15 15
.3933
.4142
.3962
.0350
.0310
.0391
IN GRP. MEAN STAND DEV
IS .4025 .0309
>>> TEI - RT. TESTIS <<<
15 15 15
.3922
.4160
.3944
.0350
.0290
.0414
14 IS
14 .5409 .0305
15 .5199 .0207
14 A B . 15
6.7292 4.0714
.4939
.2734
14 .3739 .0191
IS .3955 .0319
14 .4179 .0475
IS .4139 .0299
14 .4309 .0359
15 .4113 .0274
O O
P GO
HWI 6329-100 PRINTED: ll-OCT-91 TABLE.PAGE: 14.1
HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U S A .
TABLE 14 SUMMARY OF ORQAN-TO-BODY WEIGHT PERCENTAGES
S WEEK SACRIFICE
TABLE INCLUDES: SEX-ALL;GROUP-ALL ;WEEKS-ALL DEATH-1.SUBSET-ALL
SEX: GROUP: NUMBER
15
...... MAL E... .....
...4 ...
15 15 15
IN GRP.. MEAN. STAND DEV:
15 .6530 .0545
>>> SO 15
.6472 .0857
ACCESS/SEX 15
IOSRQ
<<<
.7122
.6710
.0910
.0769
14 14
.6956 .0579
---6-~ 15 15 .6409 .0943
HWI 6329-100 PRINTED: U-OCT-91 TABLE.PAGE: 14.2
__^trSTOD
HAZLETON LABORATORIES AMERICA, INC. MADISON. WISCONSIN U S A.
TABLE 15 SUMMARY OF ORQAN-TO-BODY WEIGHT PERCENTAGES
1 WEEK SACRIFICE
TABLE INCLUDES: SEX-ALL GROUP-ALL ;WEEKS-ALL DEATH-2;SUBSET-ALL
SEX: GROUP NUMBER
........................... MALE
--3---
--4---
15 IS IS 15
IN GRP. MEAN
STAND DEV
15 .4436. .0311
>>> BR - BRAIN <<<
15 15 IS
.4304
.4445
.4519
.0294
.0349
.0274
IN GRP. MEAN
STAND DEV
15 3.7451
.2450
>>> LI - LIVER <<<
15 15 15 A
3.6394 4 1240 5.1344
.3334
.3702
.5317
IN GRP. MEAN
STANO DEV
15 .3352 .0249
>>> LU - LUNG <<<
IS 15
.3355
.3261
.0241
.0246
15 .3194 .0271
IN GRP. MEAN
STAND DEV
15 .3561 .0291
>>> TEO - LF. TESTIS <<<
IS 15 15
.3501
.3536
.3642
.0243
.0429
.0342
IN GRP. MEAN
STAND DEV
15 .3609 .0254
>>> TEI - RT. TESTIS <<<
15 15 15
.3503
.3592
.3451
.0323
.0242
.0594
15 15
.4565 .0374 15 A B 6.0547 .7149 15 .3321 .0415 15 .3899 .0404 15 .3620 .0342
IS 15
.4654 .0292 15 3.7544 .3705 IS .3443 .0325 15 .3636 .0279 14 .3641 .0254
HUI 6329-100 PRINTED: U-OCT-91 TABLE.PAGE : 15.1
^ etstoo
HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U S A .
TABLE 15 SUMMARY OF ORGAN-TO-BODY WEIGHT PERCENTAGES
I WEEK SACRIFICE
TABLE INCLUDES: SEX-ALL;GROUP-ALL;WEEKS-ALL DEATH-2.SUBSET-ALL
SEX GROUP NUMBER
---1--15
---2--15
.......MALE---3........ . 4 . . .
15 15
--5--15
IN GRP. MEAN STAND DEV
15 .6471 .0557
>>> SO - ACCESS/SEX ORG <<<
15 15 IS 15
.6419
.7340
.6720
.6225
.0139
.0900
.0725
.0562
-- 1-- 15 IS .6439 .1090
HWI 6329-100 PRINTED: ll-OCT-91 TABLE.PAGE 1 5 2
C8T0.3
HAZLETON LABORATORIES AMERICA. INC MADISON. WISCONSIN U S A.
T A B U 16 SUMMARY OF ORGAN-TO-BODY WEIGHT PERCENTAGES
14 WEEK SACRIFICE
TABLE INCLUDES: SEX-ALL;GROUP-ALL;WEEKS-ALL DEATH-T,SUBSET-ALL
SEX
GROUP
---2---
--3---
-.4 ...
NUMBER
15
15
15
15
IN GRP. MEAN
STAND DEV
15 .3944. .0377
>>> BR - BRAIN <<<
14 15 15
.4037
.3951
.3954
.0613
.0296
.0401
IN GRP. MEAN STAND DEV
15 3.5293
.2101
>>> LI - LIVER <<<
14 A
15
15 A
3 235* 3.61*3 4.2106
.3001
.323*
.5609
IN GRP. MEAN STAND DEV
15 .2961 .0239
>>> LU - LUNG <<<
14 15
.2192
.2*56
.0329
.0329
15 .3016 .034*
IN GRP MEAN STAND DEV
15 3162 0292
>>> TEO - L F . TESTIS <<<
14 15 IS
3124
.3234
3327
.0366
.0343
.0322
IN GRP MEAN
STAND DEV
15 .3160 .0296
>>> TE1 - RT. TESTIS <<<
14 15 15
3295
.31*9
.3325
.0392
.0305
.0321
15 15
14 .4466 .0713
15 .4250 .0111
14 A B
15
5.4947 . 3.2347
.1403
.2274
14 .3136 .0357
15 .3159 .0247
14 A .3653 .0416
15 .3400 .0224
14 A .3610 .0464
15 .3360 .0216
N
HWI 6329-100 PRINTED: 11-OCT-91 TABLE.PAGE : 16.1
HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U S A
TABLE 16 SUMMARY OF ORGAN-TO-BOOY WEIGHT PERCENTAGES
14 WEEK SACRIFICE
TABLE INCLUDES: SEX-ALLGROUP-ALL:WEEKS-ALL DEATH-T;SUBSET-ALL
SEX:
E..... ---
GROUP:
NUMBER:
15
15
1S
15
IN GRP . MEAN: STAND DEV:
15 .6327 .0937
>>> 14
SO
- ACCESS/SEX ORG IS 15
<<<
.6273
.6454
.6361
.0641
.1221
.1240
IS 14
.6910 .0946
-- Q-15
15 .6470 .1241
HWI 6329-100 PRINTED. ll-OCT-91 TABLE.PAGE : 16.2
G0183
CJ
COIS
HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U S A .
TABLE 17 SUMMARY OF ORQAN-TO-BOOY WEIGHT PERCENTAGES
22 WEEK SACRIFICE
TABLE INCLUDES: SEX-ALLGROUP-ALL :WEEKS-ALL DEATH-U;SUBSET-ALL
SEX ..... MALE
GROUP -- -1--
NUMBER
10
10
10
10
8 IN GRP. MEAN STAND DEV
10 .3720 .0336
>>> 8R - BRAIN <<<
10 10 10
.3727
.3543
.3647
.0311
.0397
.0320
IN GRP. MEAN
STAND DEV
10 3.2077
.1976
>>> LI - LIVER <<<
10 10 10
3.0416 3.0160 3.4661
.1717
.2676
.4276
IN GRP. MEAN STAND DEV
10 .2776 .0117
>>> LU - LUNG <<<
10 10
.2675
.2701
.0169
.0406
10 .2621 .0214
IN GRP. MEAN STAND DEV
10 .2946 .0261
>>> TEO - LF. TESTIS <<<
10 10 10
.2904
.2761
.2850
.0266
.0345
.0431
IN GRP. MEAN STAND DEV
10 .3054 .0357
>>> TEI - RT. TESTIS <<<
10 10 10
.2912
.2674
.2996
.0306
.0259
.0375
10 10
.3904 .0624 10 3.3642 .3538 10 .2906 .0447 10 .3210 .0499 10 .3167 .0455
HWI 6329-100 PRINTED: 15-OCT-91 TABLE.PAGE: 17.1
HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U S A .
TABLE 17 SUMMARY OF ORGAN-TO-BOOY WEIGHT PERCENTAGES
22 WEEK SACRIFICE
TABLE INCLUDES: SEX>ALL:GROUP*ALL ;WEEKS-ALL DEATH*U;SUBSETALL
SEX .......MALE-
GROUP
---2--- ---3........ -4--- --5---
NIMBER
10
10
10
10
10
9 IN GRP. MEAN
STAND DEV
10 .6145 .0714
>>> SO - ACCESS/SEX ORG <<<
10 10 10
.6057
.5714
.6590
.0919
.0179
.0594
10 .6792 .1950
HWI 6329-100 PRINTED: 15-OCT-91 TABLE.PAGE: 17.2
VT90
in
HAZLETON LABORATORIES AMERICA. INC. MADISON, WISCONSIN U S A .
TABLE 11 SUMMARY OF ORGAN-TO-BRAIN WEIGHT RATIOS
5 WEEK SACRIFICE
TABLE INCLUDES. SEX-ALL;GROUP*ALL;WEEKS-ALL DEATH-1;SUBSET-ALL
SEX GROUP NUMBER
-- 1-- 15
-- 2-15
..... MALE --4--
15 15
IN GRP. MEAN STAMI DEV
15 1 0000
.0000
>>> BR - BRAIN <<<
15 15 15
1.0000 1.0000 1.0000
.0000
.0000
.0000
IN GRP. MEAN STAND DEV
15 7.1102
.9350
>>> LI * LIVER <<< 15 15 ISA 7.6323 1 7711 11 2764
.9170 1.1310 1.3499
IN GRP. MEAN STAND DEV
15 .7309 .0969
>> LU - LUNG <<<
15 15 15
.7564
.7063
.7242
.0575
.0573
.0452
IN GRP. MEAN STAND DEV
15 .7932 .0541
>>> TEO - LF. TESTIS <<<
15 15 15
.1015
.1064
.7713
.0794
.0739
.0513
IN GRP. MEAN STAND DEV
15 .7971 .0562
>>> TEI - RT. TESTIS <<<
15 IS 15
.7990
.1095
.7679
.0742
.0644
.0510
-- 5 -14 14 1.0000 .0000 14 A B
12.4713 1 0996 14 .6925 .0313 14 .7734 .0152 14 .7971 .0604
15 15 1.0000
.0000 15 7.1656
.6950 15
.7631 .0659 IS .1000 .0767 15 .7951 .0771
o>
HWI 6329-100 PRINTED. 11-OCT-91 TABLE.PAGE: 11.1
HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN US.A.
TABLE II SUMMARY OF ORGAN-TO-BRAIN WEIGHT RATIOS
S WEEK SACRIFICE
TABLE INCLUDES: SEX-ALL;GROUP-ALL:WEEKS-ALL DEATH-1;SUBSET-ALL
SEX: GROUP: NUMBER:
1S
-- 2--IS
...... MAL E ......... 15 15
IN G R P .: MEAN: STAND DEV.
IS 1.2925
.091
>>> SO IS 1.3224
.1743
ACCESS/SEX ORG <<<
15 IS
1.3897
1.3098
.2163
.1497
14 14 1.2900
.1321
15 IS 1.2333
.1678
HWI 6329-100 PRINTED: 11-OCT-B1 TABLE.PAGE: 11.2
or>
05
v4
r
0184
HAZLETON LABORATORIES AMERICA, INC. MADISON. WISCONSIN U S A .
TABLE 10 SUMMARY OF ORQAN-TO-BRAIN WEIGHT RATIOS
I WEEK SACRIFICE
TABLE INCLUOES: SEX-ALL;GROUPALL;WEEKS-ALL DEATH-2;SUBSET-ALL
SEX
GROUP
--4--
NUMBER
15
15
15
15
IN GRP. MEAN STAND DEV
15 1.0000'
.0000
>>> BR - BRAIN <<<
15 15 15
1.0000 1.0000 1.0000
.0000
.0000
.0000
IN GRP MEAN
STAND DEV
15 1.4990 1.0685
>>> 15
LI
- LIVER 15
<<<
15A
8 5253 9.3301 11.4408
1.2877
1.1031
1.5808
IN GRP. MEAN STAND DEV
15 .7571 .0536
>>> LU - LUNG <<<
15 15
.7799
.7377
.0455
.0795
15 .7083 .0518
IN GRP. MEAN
STAND DEV
15 .1042 .0614
>>> TEO - LF. TESTIS <<<
15 15 15
.8136
.8004
.8172
.0395
.1154
.0864
IN GRP. MEAN STAND DEV
15 .1153 .0601
>>> TE1 - RT. TESTIS <<<
15 15 15
.8140
.8117
8543
.0517
.0727
1342
---5 -- 15 15 1.0000 .0000 ISA B
13.3008 1.5175 15 .7295 .0929 15 .8111 .0722 15 .7939 .0604
15 15 1.0000
.0000 15 8.1178 1.1411 15
.7485 .0556 15 .7831 .0672 14 .7823 .0727
CD
HWI 6329-100 PRINTED: U-OCT-91 TABLE.PAGE. 19.1
HAZLETON LABORATORIES AMERICA, INC. MAOISON, WISCONSIN U S A .
TABLE IB SUMMARY OF ORQAN-TO-BRAIN WEIGHT RATIOS
I WEEK SACRIFICE
TABLE INCLUDES: SEX-ALL;GROUPALL;WEEKS-ALL DEATH-2;SUBSET-ALL
SEX: GROUP: NUMBER:
15
....... MAL E......... ---5 -- -
15 15 1S IS
IN GRP. : MEAN: STAND DEV:
IS 1.4672
.1710
>>> SO IS 1.4939
.1163
ACCESS/SEX ORG <<<
15 15
1.6524
1.4905
.1631
.1662
IS 1.3695
.1361
15 IS 1.3164
.2390
HWI 6329-100 PRINTED: U-OCT-91 TABLE.PAGE: 1 9 2
O o
%o
4
HAZLETON LABORATORIES AMERICA. INC. MADISON, WISCONSIN U S A.
TABLE 20 SUMMARY OF ORGAN-TO-BRAIN WEIGHT RATIOS
14 WEEK SACRIFICE
TABLE INCLUDES: SEX*ALL;GROUP-ALL;WEEKS-ALL DEATHT;SUBSET-ALL
SEX GROUP NUMBER
IS
......MALE IS 15 15
IN GRP. MEAN STAND DEV
IS 1.0000
.0000
>>> BR - BRAIN <<<
14 15 15
1.0000 1.0000 1.0000
.0000
.0000
.0000
IN GRP. MEAN
STAND DEV
15 9.0327 1.2007
>> LI - LIVER <<< 14 15 IS 6.1901 9 4115 10.7811 1.5593 1.3310 1.9614
IN GRP. MEAN STAND DEV
IS .7557 .0609
>>> LU - LUNG <<<
14 15
.7259
.7264
.1075
.0962
15 .7632 .0496
IN GRP. MEAN STAND DEV
IS .1053 .0723
>>> TEO - LF. TESTIS <<<
14 15 15
.7644
.6191
.8430
.1209
.0655
.0498
IN GRP. MEAN
STAND DEV
15 .1044 .0701
>>> TE1 - RT. TESTIS <<<
14 15 15
.6300
8074
.8428
.1526
.0532
.0555
-.-5 -- 15 14 1.0000 .0000 14 A B
12.6121 2.6823
14 .7096 .0686
14 .8255 .0849
14 .8154 .0759
15 15 1.0000
.0000 15 7.6352
.7740 IS
.7433 .0482 15 .8008 .0544 15 .7908 .0441
HW1 6329-100 PRINTED: 15-OCT-fll TABLE.PAGE : 20.1
HAZLETON LABORATORIES AMERICA. INC. MADISON, WISCONSIN U S A.
TABLE 20 SUMHARY OF ORGAN-TO-BRAIN WEIGHT RATIOS
14 WEEK SACRIFICE
TABLE INCLUDES: SEX*ALLGROUP'ALL;WEEKSALL DEATH-T;SUBSET.ALL
SEX: GROUP: NUMBER:
IS
-- -2--is
IN GR P.. MEAN:
STAND DEV:
15 1.6064
.2047
>>> SO 14 1.5795
.2402
E ........
...4 ...
15 15
ACCESS/SEX ORG <<<
15 15
1.6211
1.6014
.2354
.2191
15 14 1.5140
.3327
15 15 1.5261
.3019
HWI 6329-100 PRINTED: 15-OCT-91 TABLE.PAGE: 20 2
C0184
09
HAZLETON LABORATORIES AMERICA. INC. MADISON, WISCONSIN U S A .
TABLE 21 SUMMARY OF ORQAN-TO-BRAIN WEIGHT RATIOS
22 WEEK SACRIFICE
TABLE INCLUDES: SEX-ALL:GROUP-ALL;WEEKS-ALL DEATH-U,SUBSET-ALL
SEX GROUP NUMBER
-- 1-- 10
-- 2--10
--3......... 4--10 10
IN GRP. MEAN STAND DEV
10 1.0000
.ooop
>>> BR - BRAIN <<<
10 10 10
1.0000 1.0000 1.0000
.0000
.0000
.0000
IN GRP. MEAN STAND DEV
10 7277 1.3117
>>> LI - LIVER <<<
10 10 10
2047 I.Stll 9.5071
.7112 1.0599
.<621
9 IN GRP. MEAN STAND DEV
10 .7490 .0512
>>> LU - LUNG <<<
10 10
.7751
.7626
.0645
.0135
10 .7763 .0611
t IN GRP. MEAN STAND OEV
10 .7952 .0799
>>> TEO - LF. TESTIS <<<
10 10 10
.715
.7903
.7S2I
.0610
1042
.1097
IN GRP. MEAN
STAND DEV
10 .1243 .0966
>>> TE1 - RT. TESTIS <<<
10 10 10
7130
(161
.1229
.0746
.0152
.0917
10 10 1.0000
.0000 10 (.7300 1.1471 10
.745 .0434 10 .1242 .0627 10 .1140 .0563
HWI 6329-100 PRINTED: 15-OCT-91 TABLE.PAGE: 21.1
HAZLETON LABORATORIES AMERICA. INC. MADISON, WISCONSIN U.S.A.
TABLE 21 SUMMARY OF ORGAN-TO-BRAIN WEIGHT RATIOS
22 WEEK SACRIFICE
TABLE INCLUDES. SEX-ALL;OROUP-ALL;WEEKS-ALL OEATH-U;SUBSET-ALL
SEX: GROUP: NUMBER:
I11
I11i-111*o
1t 11
---2--10
...... MAL E......... ---3---
10 10
IN GRP. : MEAN :
STAND DEV.
10 1.6634
.2255
>>> SO - ACCESS/SEX ORG <<<
10 10 10
1.6271 1.6320 1.6151
.2294
.1649
.1973
10 10 1.7299
.3199
HWI 6329-100 PRINTED: 15-OCT-91 TABLE.PAGE: 2 1 2
HAZLETON LABORATORIES AMERICA. INC. MADISON, WISCONSIN U S A .
TABLE 22 INCIDENCE OF MACROSCOPIC OBSERVATIONS
S WEEK SACRIFICE
TABLE INCLUDES: SEX-ALL;CROUP-ALL;WEEKS-ALL DEATH-1:SUBSET-ALL
ORGAN AND KEVWORD(S) OR PHRASE ** TOP OF LIST * GENERAL COMMENT (GC| ........
-- N U M B E R -O F -A N SEX: GROUP: -1NUMBER: IS 15 15 15 14 IS
NUMBER EXAMINED: 15 15 IS IS 14 15
ALL TISSUES/ORGANS NORMAL
14 13 14 13 11 13
BRAIN (8R) ............................................ NUMBER EXAMINED: 15 IS 15 15 14 15 NOT REMARKABLE: 15 IS IS 15 14 15
LUNG fLU) ............................................. NUMMBBEERR' EXAMINED. 15 15 15 15 14 IS NOT REHARKA8LE: IS 15 15 15 14 15
LIVER (LI) ............................................ NUMBER EXAMINED: 15 IS IS 15 14 IS NOT REMARKABLE: 15 15 15 15 14 IS
LF. TESTIS (TEO) .
CONTAINS FLUID PROSTATE (PR) ...
NODULAR
........................... .........................
NUMBER EXAMINED: 15 15 15 15 14 IS NOT REMARKABLE: 15 15 IS 15 13 15 000 0 10
NUMBER EXAMINED: IS 15 IS 15 14 IS NOT REMARKABLE: 15 15 15 15 14 14 00000 1
o 00
HWI 6329-100 PRINTED: 1S-OCT-91 TABLE.PAGE: 22.1
(fSSTQO
HAZLETON LABORATORIES AMERICA. INC. MADISON, WISCONSIN U S A .
TABLE 22 INCIDENCE OF MACROSCOPIC OBSERVATIONS
5 WEEK SACRIFICE
TABLE INCLUDES: SEX-ALL;GROUP-ALL:WEEKS-ALL DEATH-1:SUBSET.ALL
ORQAN AND KEYWORD(S) OR PHRASE .......... ...... *----------SEMINAL VESICLE (SV) ......... .......................
-- NUM BER -0 F -A N
SEX:
---MALE---
GROUP: -1- -2- -3- -4- -5- -6-
NUMBER: --IS-- -15- -1m5- -15-- -14-- -15-
NUMBER EXAMINED: 15 15 15 15 14 IS NOT REMARKABLE: 15 15 15 IS 14 15
COAGULATING GLD (CGI ......... ....................... NUMBER EXAMINED: 15 15 15 15 14 15 NOT REMARKABLE: 15 IS 15 15 14 IS
URETHRA (UR) .................. ....................... NUMBER EXAMINED: IS 15 15 15 14 15 NOT REMARKABLE. 15 15 15 IS 14 15
SKIN (SKI ..................... ....................... NUMBER EXAMINEO: IS 15 IS 15 14 15 NOT REMARKABLE: 15 IS 14 15 14 IS
CRUSTED AREA(S)
00 100 0
KIDNEY(S) (KD) ............... ....................... NUM8ER EXAMINED: 15 15 15 15 14 15 NOT REMARKABLE: 14 13 15 13 11 14
LARGE PELVIS(ES) SEMIFLUID MATERIAL-PELVIS
12023 1 0000 10
SPLEEN (SP| ...................
................. NUMBER EXAMINED: 15 15 15 IS 14 15 NOT REMARKABLE: 15 15 15 14 14 15
RAISED FOCUS)I(/AREA(S)
000 10 0
oo
in
HWI 6329-100 PRINTED: 15-0CT-91 TABLE.PAGE: 22.2
HAZLETON LABORATORIES AMERICA, INC. MADISON, WISCONSIN U S A .
TABLE INCLUDES: SEX*ALL;GROUPALL;WEEKSALL DEATH!;SUBSET-ALL
ORGAN AND KEYWORD!S) OR PHRASE MESENTERIC LN (MS) ......... MANDIB/CERV LH (MN) ........ LUMBAR/ILIAC LN |LM) ....... ** END OF LIST *
TABLE 22 INCIDENCE OF MACROSCOPIC OBSERVATIONS
5 WEEK SACRIFICE
HWI 6329-100 PRINTED: 15-OCT-91 TABLE.PAGE: 22.3
-NUMBER-OF-ANIHALS-AFFECTED-
SEX:
---MALE
GROUP: -i- -2- -3- -4- -5- -6-
NUMBER: -IS-
15
-
IS
IS"
14
--S-
IS
m
NUMBER EXAMINED: IS 15 15 IS 14 IS NOT REMARKABLE: 15 IS IS IS 14 15
NUMBER EXAMINED: IS IS IS IS 14 IS NOT REMARKABLE: IS IS IS 15 14 IS
NUMBER EXAMINED: IS 15 IS IS 14 15 NOT REMARKABLE: IS 15 15 15 14 15
6
fc* Qf)
coo>
m
HAZLETON LABORATORIES AMERICA, INC. MADISON, WISCONSIN U S A .
TABLE INCLUDES: SEX-ALL;GROUP-ALL WEEKS-ALL DEATH-2;SUBSET-ALL
ORGAN AND KEYWORD!S) OR PHRASE * TOP OF LIST * GENERAL COMMENT (GC) .......
ALL TISSUES/ORGANS NORMAL MALOCCLUSION BRAIN (BR| ................
LUNG |LU) ................. MOTTLED
LIVER (LI| ................ DIFFUSELY DARK
LF. TESTIS (TEO) ......... SMALL
PROSTATE (PR| ___
o
p
05 00
cn
TABLE 23 INCIDENCE OF MACROSCOPIC OBSERVATIONS
< WEEK SACRIFICE
-- N UM B E R -0 F -A N : SEX; GROUP; -1- -2- -3- -4- -5- -eNUMBER: 15 15 15 15 15 15
NUMBER EXAMINED; 15 15 15 15 15 15
14 13 12 13 14 14 00 1000
NUMBER -EXAMINED: 15 15 IS 15 15 IS NOT REMARKABLE: 15 15 IS 15 15 15
NUMBER EXAMINED: 15 15 15 IS IS 15 NOT REMARKABLE: 14 IS 15 15 15 15 10000 0
NUMBER EXAMINED: 15 15 15 IS 15 15 NOT REMARKABLE: IS 15 15 15 14 IS 0000 10
NUMBER EXAMINED: 15 15 15 15 15 15 NOT REMARKABLE: 15 IS 14 15 15 15 00 10 00
NUMBER EXAMINED: 15 IS 15 15 IS 15 NOT REMARKABLE: 15 IS 15 15 15 15
HWI 6329-100 PRINTED. 15-OCT-91 TABLE.PAGE 2 3 1
I-
G0135
HAZLETON LABORATORIES AMERICA. INC. MADISON, WISCONSIN U S A .
TABLE 23 INCIDENCE OF MACROSCOPIC OBSERVATIONS
I WEEK SACRIFICE
TABLE INCLUDES: SEX-ALL;GROUPALL ;WEEKS-ALL DEATH-2:SUBSET-ALL
-- N UM B E R -0 F -A N
SEX:
--MALE---
GROUP : -1- -2- -3- -4- -5- -e-
ORGAN AND KEYWORD(S ) OR PHRASE
NUMBER : -m15- -m15- -IS- 15- -a15-- -is-
SEMINAL VESICLE (SV) ....................... ......... NUMBER EXAMINED: 15 15 15 15 15 is NOT REMARKABLE: 15 15 15 15 IS 15
COAGULATING OLD (CG| ....................... ......... NUMBER EXAMINED: 15 15 IS 15 15 15 NOT REMARKABLE: 15 15 15 IS 15 15
URETHRA (UR) ............................... ......... NUMBER EXAMINED: 15 15 15 15 15 15 NOT REMARKABLE: 15 15 15 15 15 15
SKIN (SK) ................................... ......... NUMBER EXAMINED: 15 IS 15 15 15 15 NOT REMARKABLE: 15 15 15 15 IS IS
KIDNEY(S) |KD) ............................. ......... NUMBER EXAMINED: 15 15 15 15 15 15 NOT REMARKABLE: 15 15 14 15 15 15
LARGE PELVIS(ES)
00 1000
SPLEEN (SP) ........ ........................ ......... NUMBER EXAMINED: 15 15 15 15 IS 15 NOT REMARKABLE: 15 15 15 15 15 15
HWI 6329-100 PRINTED: 1S-0CT-91 TABLE.PAGE: 23.2
CCOo
HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U S A.
TABLE INCLUDES: SEX-ALL.GROUP-ALL;WEEKS-ALL DEATH-2;SUBSET-ALL
ORGAN AM) KEYWORD!S) OR PHRASE MESENTERIC LN (MS) .........
MOTTLED MANDIB/CERV LN (MN| ........
LARGE LUMBAR/ILIAC LN (LM) ....... ** END OF LIST *
TABLE 23 INCIDENCE OF MACROSCOPIC OBSERVATIONS
8 WEEK SACRIFICE
HWI 6329-100 PRINTED: 1S-OCT-91 TABLE.PAGE : 23.3
-- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: ............ MALE............ GROUP: -1- -2- -3- -4- -5- -6NUMBER: -1S --15- w15- --1S-- --15-- --15--
NUMBER EXAMINED: IS 15 IS 15 15 15 NOT REMARKABLE: 15 15 15 14 15 15 000 100
NUMBER EXAMINED: 15 15 15 15 IS 15 NOT REMARKABLE: 15 13 IS 14 15 14 020 10 1
NUMBER EXAMINED: 15 15 15 15 15 15 NOT REMARKABLE: IS 15 IS IS IS 15
ASSTGJ
00
to
HAZLETON LABORATORIES AMERICA. INC MADISON. WISCONSIN U S A .
TABLE 24 INCIDENCE OF MACROSCOPIC OBSERVATIONS
14 WEEK SACRIFICE
TABLE INCLUDES SEX-ALL.GROUP-ALL;WEEKS-ALL DEATH-T;SUBSET-ALL
ORGAN AND KEYWORD)S) OR PHRASE ** TOP OF LIST ** GENERAL COFWENT (GC| ............
....................
N U M BER - 0 F - A N
SEX:
---MALE
GROUP : -1- -2- -3- -4- -5- -8-
NUMBER: --15- --15- --15-- -15- a15-- -IS
NUMBER EXAMINED: 15 15 15 15 15 IS
ALL TISSUES/ORGANS NORMAL
14 14 15 13 13 IS
BRAIN (BR) ....................... .................... NUMBER EXAMINED: 15 15 15 15 IS 15 NOT REMARKABLE: 15 15 15 15 15 IS
LUNG (LU) ........................ .................... NUMBER EXAMINED: 15 IS 15 15 15 IS NOT REMARKABLE: 15 15 15 15 15 15
LIVER (LI) ....................... .................. NUMBER EXAMINED: 15 15 15 15 15 IS NOT REMARKABLE: 15 15 15 15 15 15
LF. TESTIS (TEO) ................. .................... NUMBER EXAMINED: 15 15 IS 15 15 IS NOT REMARKABLE: 15 14 15 IS IS 15
SMALL
0 10000
PROSTATE (PR) ...
......... .................... NUMBER EXAMINED: 15 15 IS 15 15 15 NOT REMARKABLE: 15 15 15 14 15 15
MASS(ES)
0 0 0 10 0
HWI 6329-100 PRINTED: 15-OCT-91 TABLE.PAGE: 24 1 AFFECTEO---
f'SSTOi
to
o
HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U S A .
TABLE INCLUDES SEX*ALL GROUP-ALL;WEEKS-ALL DEATH*T;SUBSET-ALL
ORGAN AND KEYWORD!S) OR PHRASE SEMINAL VESICLE (SV| ....... COAGULATING GLD |CG) ....... URETHRA (UR) ............... SKIN (SK) .................. KIDNEY!S) (KD) .............
LARGE PELVIS(ES) SPLEEN ISP) ....... MESENTERltUN (MS) .
TABLE 24 INCIDENCE OF MACROSCOPIC OBSERVATIONS
14 WEEK SACRIFICE
... N U M B E R - 0 F - A N
GROUP: -1- -2- -3- -4- -5- -6-
NUMBER: 15 15 15 - -*- --
15 15 15
-- -- -
--
NUMBER EXAMINED: 15 15 15 15 15 15 NOT REMARKABLE: 15 15 15 15 15 15
NUMBER EXAMINED: 15 IS IS 15 15 15 NOT REMARKABLE: 15 15 15 15 15 IS
NUMBER EXAMINED: 15 IS 15 IS 15 IS NOT REMARKABLE: 15 15 15 15 15 15
NUMBER EXAMINED: 15 15 15 15 15 IS NOT REMARKABLE: 15 15 15 15 15 15
NUMBER EXAMINED: 15 15 15 15 IS IS NOT REMARKABLE: 15 15 15 14 14 15 000 110
NUMBER EXAMINED: 15 15 15 15 15 IS NOT REMARKABLE: 15 15 15 15 15 15
NUMBER EXAMINED: 15 15 15 15 15 15 NOT REMARKABLE: 15 15 15 15 15 15
HWI 6329-100 PRINTED: 15-OCT-91 TABLE.PAGE: 24.2
HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U S A.
TABLE INCLUDES. SEX-ALL;GROUP-ALL.WEEKS-ALL DEATH-T:SUBSET-ALL
ORGAN AND KEYWORD)S) OR PHRASE MANDIB/CERV LN |MN) ........
LARGE LUMBAR/ILIAC LN |LM) ....... * END OF LIST **
TABLE 24 INCIDENCE OF MACROSCOPIC OBSERVATIONS
14 WEEK SACRIFICE
-- N UM B E R -0 F -A N SEX: GROUP: -1- -2- -3- -4- -5- -6NUMBER: IS IS 15 IS IS IS NUMBER EXAMINED: 15 IS 15 IS 15 15 NOT REMARKABLE: 14 15 IS 15 14 15
1000 10 NUMBER EXAMINED: IS 15 IS 15 15 15
NOT REMARKABLE: IS IS 15 15 IS IS
HWI 6329-100 PRINTED: 1S-OCT-91 TABLE.PAGE: 24.3 A F F E C T E D ---
CC1S57
vroo
0SSTO3
HAZLETON LABORATORIES AMERICA, INC MADISON, WISCONSIN U S A.
TABLE INCLUDES: SEXALL;GROUP<ALL;WEEKSALL DEATH*U;SUBSET-ALL
ORGAN AND KEYWORD!S) OR PHRASE * TOP OF LIST GENERAL COMMENT JGC) .......
ALL TISSUES/ORGANS NORMAL ANIMAL THIN ANIMAL OBESE BRAIN (BR| .................. LUNG (LU| LIVER |LI 1 IF. TESTIS (TEO) .. PROSTATE (PR) MASS(ES)
uO>J
TABLE 25 INCIDENCE OF MACROSCOPIC OBSERVATIONS
22 WEEK SACRIFICE
-- N UM B E R -0 F SEX: GROUP: -1- -2- -3- -4- -5NUMBER: 10- --10-- -10- -10 -10-- NUMBER EXAMINED: 10 10 10 10 10
10 9 9 0000 1 00 100 NUMBER EXAMINED: 10 10 10 10 10 NOT REMARKABLE: 10 10 10 10 10 NUMBER EXAMINED: 10 10 10 10 10 NOT REMARKABLE: 10 10 10 10 10 NUMBER EXAMINED: 10 10 10 10 10 NOT REMARKABLE: 10 10 10 10 10 NUMBER EXAMINED: 10 10 10 10 to NOT REMARKABLE: 10 10 10 10 10 NUMBER EXAMINED: 10 10 10 10 10 NOT REMARKABLE: 10 10 10 10 9 0000 1
HWI 6329-100 PRINTED: 15-OCT-91 TABLE.PAGE : 25.1 A F F E C T E D ---
bSTOi)
HAZLETON LABORATORIES AMERICA. INC MADISON, WISCONSIN U S A .
TABLE 25 INCIDENCE OF MACROSCOPIC OBSERVATIONS
22 WEEK SACRIFICE
TABLE INCLUDES: SEX'ALL.GROUP'ALL:WEEKS-ALL DEATHU;SUBSET-ALL
O-R-G-A-N -A-ND--K-E-Y-W-O-RD-|-S-|--O-R--P-HR-A-SE
SEMINAL VESICLE (SV| ........ ........................
-- NUM BER -0 F -AN
SEX:
---MALE
GROUP: -1- -2- -3- -4- -5- -6-
NUMBER: 10- -10- -10 -s10- --*10-- -*0-
NUMBER EXAMINED: 10 10 10 10 10 NOT REMARKABLE: 10 10 10 10 10
0 0
COAGULATING OLD (CG)
........................ NUMBER EXAMINED: 10 10 10 10 10 NOT REMARKABLE: 10 10 10 10 10
0 0
URETHRA |UR) ................ ........................ NUMBER EXAMINED: 10 10 10 10 10 NOT REMARKABLE: 10 10 10 10 10
0 0
SKIN (SK| ................... ........................ NUMBER EXAMINED: 10 10 10 10 10 NOT REMARKABLE: 9 10 10 10 10
ABRASIONISl/ULCERATIONIS)
10 000
KIDNEY)SI (KD) .............. ........................ NUMBER EXAMINED: 10 10 10 10 10 NOT REMARKABLE: 10 10 10 9 10
LARGE PELVIS(ES) SEMIFLUID MAlERIAL-PELVIS
0 0 0 10 0 0 0 10
SPLEEN (SP| ...............
................... NUMBER EXAMINED: 10 10 10 10 10 NOT REMARKABLE: 9 10 10 10 10
CYST|S)
1000 0
0 0 0 0 0 0 0 0 0 0
HWI 6329-100 PRINTED: 15-OCT-91 TABLE.PAGE: 25.2
HAZLETON LABORATORIES AMERICA, INC. MAOISON, WISCONSIN U S A .
TABLE INCLUDES: SEX'ALL;GROUP.ALL;WEEKSALL DEATH.U.SUBSET ALL
ORGAN AND KEVWOROfS) OR PHRASE MESENTERIC LN (MS) .........
MANDIB/CERV LN (MNJ ........ LARGE
LUMBAR/1LIAC LN (LM) ....... LARGE
END OF LIST *
TABLE 25 INCIDENCE OF MACROSCOPIC OBSERVATIONS
22 WEEK SACRIFICE
HWI 6329-100 PRINTED: 15-OCT-91 TABLE.PAGE: 25.3
-- N U M B E R - O F - A N I M A L S - A F F E C T E D ----
SEX:
---MALE
GROUP: -1- -2- -3- -4- -5- -6-
NUMBER: 10 -10 -10- -10" -a10 0--
NUMBER EXAMINED: 10 10 10 10 10 NOT REMARKABLE: 10 10 10 10 10
0 0
NUMBER EXAMINED: 10 10 10 10 10 NOT REMARKABLE: 10 10 10 10 9 0000 1
NUMBER EXAMINED: 10 10 10 10 10 NOT REMARKABLE: 10 10 10 10 9 0000 1
0 0 0 0 0 0
098TOQ
in
HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U S A .
TABLE 26 INCIDENCE OF MICROSCOPIC OBSERVATIONS
S WEEK SACRIFICE
HWI 6329-100 PRINTED: ll-OCT-91 TABLE.PAGE: 26 1
TABLE INCLUDES: SEX-ALL;GROUP-ALL;SCREEN-ALL ;WEEKS-ALL DEATH-1;FIND-ALL:SUBSET-ALL
ORGAN AND FINDING DESCRIPTION ** TOP OF LIST BRAIN (BR) .....
-- N U M B E R - 0 F - A N II M A L S - A F F E C T E D - - -
SEX:
GROUP: -1- -2- -3- -4- -5- -6-
NUMBER: 15 15- 15 -15- -m14- -15-
NUMBER EXAMINED: 15 NOT REMARKABLE: 15
15 15
15 IS
15 15
14 14
15 IS
LUNG
NUMBER EXAMINED: 15 15 15 14 14 15 NOT REMARKABLE: 15 15 15 14 14 15
LIVER
NUMBER EXAMINED: 15 15 15 15 14 15 NOT REMARKABLE: 9 T 2 0 0 10
-INFILTRATES.MONONUCLEAR CELL
-INFLAMMATION,GRANULOMATOUS
-NECROSIS,COAGULATIVE
-FATTY CHANGE
-HYPERTROPHY, HEPATOCELLULAR:
ACCENTUATED
iPPEAR TO HAVE
ARE MORE
Ro u n d e d g i v i n g the c e l l s a m o r e PLUMP" APPEARANCE EXCEPT FOR
THIS 'ENLARGED* APPEARANCE. THE
>E 'NORMAL'.
-HYPERPLASIA.BILE DUCT
5 T455 4 111 100 000 12 1 00000 1 0 0 12 15 14 0 000000
LF. TESTIS (TEO)
NUMBER EXAMINED: 15 15 15 IS 14 15 NOT REMARKABLE: 15 15 15 15 13 IS
--ATROPHY/DEGENERATION --INFLAMMATION. GRANULOMATOUS
00 00 10 000000
#58TO0
icon
T98T00
HAZLETON LABORATORIES AMERICA. INC. MADISON, WISCONSIN U S A.
TABLE 26 INCIDENCE OF MICROSCOPIC OBSERVATIONS
S WEEK SACRIFICE
TABLE INCLUDES: SEX-ALL;GROUP-ALL ;SCREEN-ALL :WEEKS-ALL DEATH-1:FIND-ALL:SUBSET-ALL
O--R-G-A-N--A-N-D--F-I-N-D-I-N-G--D-E-S-C-RI"P-T"I-O-N---------PROSTATE (PR) ..... ......................... .........
--INFLAMMATION, CHRONIC ACTIVE SEMINAL VESICLE (SV) ....................... .........
-- HUM B E R -0 F - A N
SEX:
---MALE---
GROUP: -1- -2- -3- -4- -5- -6-
NUMBER: -15-
IS-
15m --15--
14 m -
15
NUMBER EXAMINED: 15 15 14 15 14 15 NOT REMARKABLE: 15 15 14 15 14 13 000002
NUMBER EXAMINED: IS 15 14 15 14 IS NOT REMARKABLE: 15 IS 14 15 14 15
COAGULATING OLD (CG) ....................... ......... NUMBER EXAMINED: 15 15 14 15 14 15 NOT REMARKABLE: 15 IS 14 IS 14 IS
URETHRA (UR) ............................... ......... NUMBER EXAMINED: 15 IS 14 15 14 14 NOT REMARKABLE: 15 15 14 IS 14 14
KIDNEY(S) |KD) ............................. ......... NUMBER EXAMINED: 1 2 0 2 3 1 NOT REMARKABLE: 0 0 0 0 0 0
--DILATATION,PELVIC
12023 1
SKIN (SK) ..................
........ ......... NUMBER EXAMINED: 0 0 1 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0
--DERMATITIS --ULCERATION
0 0 10 0 0 000000
HWI 6329-100 PRINTED: ll-OCT-91 TABLE.PAGE : 26.2
HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U S A .
TABLE 26 INCIDENCE OF MICROSCOPIC OBSERVATIONS
S WEEK SACRIFICE
TABLE INCLUDES: SEX*ALL.GROUPALL:SCREEN-ALL;WEEKS-ALL DEATH-i,FIND-ALL.SUBSET.ALL
N U M B E R -0 F -a n :
SEX:
---MALE
GROUP: -1- -2- -3- -4- -5- -6-
ORGAN AND FINDING DESCRIPTION
NUMBER: -m15- 15 -1m5- -015- 14-- --15--
SPLEEN |SP) ....... .........................
000 100 NOT REMARKABLE: 0 0 0 l 0 0
--CVST. SEROSAL
000000
MANDIB/CERV LN (MN| .......................... ....... NUMBER EXAMINED: 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0
--HYPERPLASIA. LYMPHORETICULAR CELL
000000
MESENTERIC LN |MS) ........................... ....... NUMBER EXAMINED: 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0
LUMBAR/ILIAC LN |LM) ......... ................ ....... NUMBER EXAMINED: 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0
--HYPERPLASIA. LYMPHORETICULAR CELL ** END OF LIST
000000
HWI 6329-100 PRINTED: 1S-0CT-91 TABLE.PAGE: 26.3
98X00
0o0
^98TO0
HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U S A.
TABLE 27 INCIDENCE OF MICROSCOPIC OBSERVATIONS
t WEEK SACRIFICE
TABLE INCLINES: SEX-ALL :GROUP-ALL :SCREEN-ALL ;WEEKS-ALL DEATH-2;FIND-ALL;SUBSET-ALL
ORGAN AND FINDING DESCRIPTION ** TOP OF LIST ** BRAIN (BR) ........ ...........
--- N U M B E R - 0 F - A N :
SEX:
--MALE---
GROUP: -1- -2- -3- -4- -5- -6-
NUMBER: 1S IS 15 15 15 15
NUMBER EXAMINED. IS IS 15 IS 15 15 NOT REMARKABLE: IS IS IS IS 15 IS
LUNG (LU) .......................
NUMBER EXAMINED: 14 15 13 14 15 15 NOT REMARKABLE: 14 15 13 14 15 15
LIVER (LI 1 ......................
NUMBER EXAMINED: 15 IS 15 15 15 15 NOT REMARKABLE: 11 12 3 0 0 10
--INFILTRATES.MONONUCLEAR CELL --INFLAMMATION.GRANULOMATOUS --NECROSIS,COAGULATIVE --FATTY CHANGE --HYPERTROPHY. HEPATOCELLULAR: CHARACTERIZED BY AN ACCENTUATED
CENTRILOBULATED PATTERN IN WHICH THE HEPATOCYTES APPEAR TO HAVE A MORE HOMOGENEOUS CYTOPLASM AND THE CELL BORDERS ARE MORE ROUNDED GIVING THE CELLS A MORE 'PLUMP" APPEARANCE EXCEPT FOR THIS 'ENLARGED* APPEARANCE. THE CELLS; ARE OTHERWISE 'NORMAL'. --HYPERPLASIA.BILE DUCT
43I455 000 100 0000 10 000000 0 0 12 15 IS 0 000 00 0
LF. TESTIS |TEO) ................
NUMBER EXAMINED: IS 15 15 15 15 15 NOT REMARKABLE: 15 15 14 15 15 IS
--ATROPHY/DEGENERATION --INFLAMMATION. GRANULOMATOUS
00 10 00 000000
IOo
HWI 6329-100 PRINTEO: 1S-0CT-91 TABLE.PAGE : 27.1
^98T00 1I11111111111111111111111111111111111t11
HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U S A .
TABLE 27 INCIDENCE OF MICROSCOPIC OBSERVATIONS
I WEEK SACRIFICE
TABLE INCLUDES: SEX-ALL;GROUP-ALL:SCREEN-ALL :WEEKS-ALL DEATH-2;FIND-ALL;SUBSET.ALL
-- HUM 8 E R -0 F - A N
SEX:
--MALE---
GROUP: -1- -2- -3- -4- -5- - a -
ORGAN ANO FINDING DESCRIPTION
NUMBER: IS 15 --IS- -'15-- m15-- IS
PROSTATE (PR I ........................... ............. NUMBER EXAHINEO: IS 15 IS 15 15 15 NOT REMARKABLE: 14 15 15 IS 14 15
--INFLAMMATION. CHRONIC ACTIVE
1000 10
SEMINAL VESICLE (SV| .................... ............. NUMBER EXAMINED: 15 15 15 15 15 IS NOT REMARKABLE: 15 15 15 IS 15 15
COAGULATING GLD (CG) .................... ............ NUMBER EXAMINED: IS IS 15 15 IS 15 NOT REMARKABLE: IS 15 15 15 15 15
URETHRA (UR) ............................ ............ NUMBER EXAMINED: 15 IS 15 14 14 14 NOT REMARKABLE: IS 15 15 14 14 14
KIDNEYISI (KD) .......................... ............. NUMBER EXAMINED: 0 0 1 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0
--DILATATION.PELVIC
00 100 0
SkIN (SK| ...............
....... ............. NUMBER EXAMINED: 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0
--DERMATITIS --ULCERATION
000000 000000
oo
HWI 6329-100 PRINTED: 1S-OCT-91 TABLE.PAGE: 27.2
C018S
HAZLETON LABORATORIES AMERICA. INC MADISON, WISCONSIN U S A .
TABLE 27 INCIDENCE OF MICROSCOPIC OBSERVATIONS
I WEEK SACRIFICE
TABLE INCLUDES SEX-ALL:GROUP-ALL:SCREEN-ALLWEEKS-ALL DEATH-2 :FIND-ALL:SUBSET-ALL
ORGAN AND FINDING DESCRIPTION -----------------------SPLEEN (SP) ....... ........................ ........
--CVST. SEROSAL MANDIB/CERV LN |MN) ......................... ........
--HYPERPLASIA. LYMPHORETICULAR CELL MESENTERIC LN IMS) .......................... ........
... N U M B E R - 0 F - A N
SEX:
--MALE
GROUP : -1- -2- -3- -4- -5- -6-
NUMBER: -15. -a15<" .a15. .a15 --a15-- -aIS.
NUMBER EXAMINED: 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0 000000
NUMBER EXAMINED: 0 2 0 1 0 1 NOT REMARKABLE: 0 0 0 0 0 0 020 10 1
NUMBER EXAMINED: 0 0 0 1 0 0 NOT REMARKABLE: 0 0 0 1 0 0
LUMBAR/ILIAC LN (LM) ........................ ........ NUMBER EXAMINED: 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0
--HYPERPLASIA. LYMPHORETICULAR CELL * END OF LIST *
000000
HWI 6329-100 PRINTED: ll-OCT-91 TABLE.PAGE: 27.3 AFFECTED---
HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U S A .
TABLE 2*
INCIDENCE OF MICROSCOPIC OBSERVATIONS
14 W E E K SACRIFICE
HWI 6329-100
PRINTED: 1S-0CT-91 T A B L E .P A G E : 2 1 . 1
TABLE INCLUDES: S E X - A L L ; G R O U P - A L L ;S C R E E N - A L L W E E K S - A L L DEATH-T;FIND-ALLSUBSET-ALL
OROAN AND FINDING DESCRIPTION * T O P O F L I S T * B R A I N |BR) ......
IUNO
LIVER
-INFILTRATES.MONONUCLEAR CELL - I N F L A M M A T I O N ,G R A N U L O M A T O U S -NECROSIS.COAGULATIVE -FATTY CHANGE -HYPERTROPHY, HEPATOCELLULAR:
ROUNDED GIVING THE CELLS A MORE 'PLUMP' THIS "ENLARGED* APPEARANCE. THE CFILS 4 -HYPERPLASIA.BILE DUCT
Lf* TESTIS (TEO)
--ATROPHV/DEGENERATION --INFLAMMATION. GRANULOMATOUS
... N U M B E R - 0 F - A N I M A L S
SEX:
*rWLt
G R O U P : -1- -2- -3- -4- -5- -6-
N U M B E R : --15-- m15m --1a5-- -15" IaS- -a15
N U M B E R E X A M I N E D : 15 is 15 IS 15 15
N O T R E M A R K A B L E : IS 15 IS 15 15 15
AFFECTED---
N U M B E R E X A M I N E D : 15 15 15 15 15 15 N O T R E M A R K A B L E : IS IS 15 15 IS 15
N U M B E R E X A M I N E O : 15 IS 15 15 IS 15
N O T R E M A R K A B L E : 12 11 2 1 0 7
ACCENTUATED iPPEAR TO HAVE
ARE MORE
. EXCEPT FOR
>E ' N O R M A L * .
33e5 71 0013 0 0 0 10 100 0 00000 0 0 13 14 IS 0 000000
N U M B E R E X A M I N E D : 15 15 15 15 15 15 N O T R E M A R K A B L E : 15 14 IS IS 15 15
0 10000 0 10000
o
S8T00 111< 1111 11tt11111 1 111 1111 11 111 1 11 1 1 1 t1 1 11
HAZLETON LABORATORIES AMERICA. INC. MADISON, WISCONSIN U S A .
TABLE 21 INCIDENCE OF MICROSCOPIC OBSERVATIONS
14 WEEK SACRIFICE
HWI 6329-100 PRINTED: 14-0CT-91 TABLE.PAGE: 21.2
TABLE INCLUDES: SEX-ALL:GROUP-ALL:SCREEN-ALL :WEEKS-ALL DEATH-T:FIND-ALL:SUBSET-ALL
... N U M B E R - 0 F - A N I M A L S
SEX:
---MALE----
GROUP : -1- -2- -3- -4- -5- -6-
ORGAN AND FINOING DESCRIPTION
NUMBER: IS
--
15 --
15 ----
- aIS-
--a15-
IS
*a-
PROSTATE (PR) ..... ....................... ............ NUMBER EXAMINED: 15 IS IS is 15 15 NOT REMARKABLE: IS 15 13 14 14 14
--INFLAMMATION. CHRONIC ACTIVE
002111
SEMINAL VESICLE (SV| ............................ ............ NUMBER EXAMINED: IS IS IS IS IS IS NOT REMARKABLE: 15 IS IS IS 15 15
COAGULATING GLD fCQ) ....................... ......... NUMBER EXAMINED: 15 IS 15 15 IS IS NOT REMARKABLE: IS IS IS 15 IS IS
URETHRA (UR) ................................ ......... NUMBER EXAMINED: IS IS 15 IS IS IS NOT REMARKABLE: IS 15 IS IS IS IS
KIDNEY IS) (KD) ............................. ............ NUMBER EXAMINED: 0 0 0 1 1 0 NOT REMARKABLE: 0 0 0 0 0 0
--DILATATION.PELVIC
00 0 1 10
SKIN (SK) .......................
....... ............ NUMBER EXAMINED: 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0
-DERMATITIS --ULCERATION
00 0 0 0 0 000000
ogo
HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U S A .
TABLE 21 INCIDENCE OF MICROSCOPIC OBSERVATIONS
14 WEEK SACRIFICE
HWI 6329-100 PRINTED: 1S-0CT-91 TABLE.PAGE: 21.3
TABLE INCLUDES: S E X - A L L ;G R O U P - A L L :S C R E E N - A L L . W E E K S - A L L d e a t h - 1 , f i n d -a l l .Su b s e t -a l l
N U M B E R - 0 F - aA N I: M A L S - A F F E C T E D
SEX:
--MALE
G R OU P: -1- -2- -3- -4 - -5- -6 -
ORGAN AND FINDING DESCRIPTION
N U M B E R : 15 15 --
15
IS 15 15 " --
S P L E E N |SP> ....... ............................. ...... N U M B E R E X A M I N E D : 0 0 0 0 0 0
NOT REMARKABLE:
0
0
0
0
0
0
--CYST. SEROSAL
000000
M A N D I B / C E R V L N (MN) ................................... ...... N U M B E R E X A M I N E D :
1
0
0
0
1
0
NOT REMARKABLE:
0
0
0
0
0
0
--HYPERPLASIA, LYMPHORETICULAR CELL
100 0 10
M E S E N T E R I C LN (MS) .................................... ...... N U M B E R E X A M I N E O :
0
0
0
0
0
0
NOT REMARKABLE:
0
0
0
0
0
0
--
L U M B A R / I L I A C L N (LM) ................................. ...... N U M B E R E X A M I N E D :
0
0
0
0
0
0
NOT REMARKABLE:
0
0
0
0
0
0
--HYPERPLASIA. LYMPHORETICULAR CELL
** END OF LIST **
000000
C-018:
o
0Z.STOO
HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U S A .
TABLE 20 INCIDENCE OF MICROSCOPIC OBSERVATIONS
22 WEEK SACRIFICE
TABLE INCLUDES: SEX-ALL.GROUPALL ;SCREEN-ALL ;WEEKS-ALL DEATH-U;FIND-ALL;$UBSETALL
ORGAN AND FINDING DESCRIPTION * TOP OF LIST BRAIN |BR| ...
... N U M B E R - 0 F - a n :
SEX:
GROUP: -I- -2- -3- -4- -5- -6-
NUMBER: 10
-
10 --
- a10-
O
-- a10-- - a0-
NUMBER EXAMINED: 10 10 NOT REMARKABLE: 10 10
9 10 10 9 10 10
0 0
LUNG
NUMBER EXAMINED: 10 10 10 10 10 NOT REMARKABLE: 10 10 10 10 10
0 0
LIVER
NUMBER EXAMINED: 10 NOT REMARKABLE: s
10
5
10
6
10
1
107
-INFILTRATES.MONONUCLEAR CELL
-INF LAMMATION.GRANULOMATOUS
-NECROSIS.COAGULATIVE
-FATTY CHANGE
-HYPERTROPHY, HEPATOCELLULAR: CHARAC
ACCENTUATED
CENTRILOBULATED PATTERN IN WHICH THE HEPATOCYTES APPEAR TO HAVE
A MORE HOMOGENEOUS CYTOPLASM AND THE CELL BORDERS ARE MORE
ROUNDED GIVING THE CELLS A MORE "PLUMP' APPEARANCE. EXCEPT FOR
THIS "ENLARGED* APPEARANCE, THE CELLS ARE OTHERWISE "NORMAL" .
--HYPERPLASIA,BILE DUCT
242 1 3 20 0 0 0
1 1 1 10 0 0 10 0 00000
0 10 00
TESTIS (TEO) ..................
.......... NUMBER EXAMINED: 10 10 10 10 10 NOT REMARKABLE: 10 9 9 9 10
--ATROPHY/DEGENERATION --INFLAMMATION. GRANULOMATOUS
0 11 10 00000
0 0
0 0 0 0 0
0 0 0 0 0
cn
HWI 6329-100 PRINTED: ll-OCT-91 TABLE.PAGE: 29 1 A F F E C T E D ---
HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U S A .
TABLE 29 INCIDENCE OF MICROSCOPIC OBSERVATIONS
22 WEEK SACRIFICE
HWI 6329-100 PRINTED. 1S-OCT-91 TABLE.PAGE: 29.2
TABLE INCLUDES: SEX*AlL :GROUPALL :SCREENALL :WEEKSALL DEATH*U:FINDALL ;SUBSET*ALL
--- N U M B E R - 0 F - A N I M A L S
SEX:
---MALE---
GROUP: -1-
ORGAN AND FINDING DESCRIPTION
NUMBER: 10 10 -10 -10-- --a10-- --a0
PROSTATE (PR) ..... ........................ ........ NUMBER EXAMINED: 10 10 10 10 10 NOT REMARKABLE: - 10 10 10
--INFLAMMATION. CHRONIC ACTIVE
20002
SEMINAL VESICLE (SV| ........................ ........ NUMBER'EXAMINED: 10 10 10 10 10 NOT REMARKABLE: 10 10 10 10 10
0 0 0 0 0
COAGULATING GLD (CG) ........................ ........ NUMBER EXAMINED: 10 10 10 10 10 NOT REMARKABLE: 10 10 10 10 10
0 0
URETHRA (UR) ................................ ........ NUMBER EXAMINED: 10 10 10 10 10 NOT REMARKABLE: 10 10 10 10 10
0 0
KIDNEY(S) |KD) .............................. ........ NUMBER EXAMINED: 0 0 0 1 0 0 NOT REMARKABLE: 0 0 0 0 0 0
--DILATATION,PELVIC
0 00 10 0
SKIN |SK) ..............
.............. ........ NUMBER EXAMINED: 1 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0
--DERMATITIS --ULCERATION
10 0 0 0 0 10 0 00 0
P-.STOO
001
HAZLETON LABORATORIES AMERICA. INC MADISON, WISCONSIN U S A .
TABLE INCLUDES. SEX-ALL;GROUP-ALL:SCREEN-ALL;WEEKS>ALL DEATH-U,FIND-ALL;SUBSET-ALL
ORGAN AND FINDING DESCRIPTION SPLEEN (SP| ....... .....................
--CYST, SEROSAL MANDIB/CERV LN (MNJ ....................
--HYPERPLASIA. LYMPHORETICULAR CELL MESENTERIC LN (MS) .....................
LUMBAR/ILIAC LN (LM) ................... --HYPERPLASIA. LYMPHORETICULAR CELL
END OF LIST **
TABLE 29 INCIDENCE OF MICROSCOPIC OBSERVATIONS
22 WEEK SACRIFICE
--- N U M B E R - 0 F - A N SEX: GROUP: -INUMBER: 10 10 10 10 10 0 NUMBER EXAMINED: 1 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0
100 0 0 0 NUMBER EXAMINED: 0 0 0 0 1 0
NOT REMARKABLE: 0 0 0 0 0 0 0000 10
NUMBER EXAMINED: 0 0 0 0 0 0 NOT REMARKABLE: 0 0 0 0 0 0
NUMBER EXAMINED: 0 0 0 0 1 0 NOT REMARKABLE: 0 0 0 0 0 0 0000 10
HWI 6329-100
PRINTED: ll-OCT-91 TABLE.PAGE: 29.3 A F F E C T E D ---
^STOO
oj
C01S7J
HAZLETON LABORATORIES AMERICA. INC. MAOISON. WISCONSIN U S A .
TABLE 30 INCIDENCE OF SEVERITY OF MICROSCOPIC OBSERVATIONS
5 ' WEEK SACRIFICE
TABLE INCLUDES: SEX-ALL;GROUP-ALL;SCREEN-ALL;WEEKS-ALL DEATH-1;FIN0*ALL;SUBSET-ALL
ORGAN/TISSUE EXAMINED * TOP OF LIST ** BRAIN (BR) ........ ...................... .......... LUNG |LU) ....... ........................ .......... LIVER (LI) ................................ ..........
--INFILTRATES,MONONUCLEAR CELL
--INF LA MM AT IO N.GRANULOMATOUS
--NECROSIS,COAGULAIIVE
. --FATTY CHANGE ** CONTINUED ON NEXT PAGE
""- N U M B E R - 0 F - A N SEX: GROUP: -1- -2- -3- -4- -s- -6NUMBER: IS 15 15 15 14 15
NUMBER EXAMINED: 15 15 IS IS 14 IS
NUMBER EXAMINED: IS 15 15 14 14 15
NUMBER EXAMINED-:>
IS 10
15 1
IS 11
15 10
14 9
15 11
1> 3 S 4 5 4 3
2> 2 2 0 0 1 1
TL> 15 15 15 IS 14 15
-> 14 14 14 14 14 15 1> 0 1 1 0 0 0 2> 0 0 0 1 0 0 3> 1 0 0 0 0 0 TL> 15 IS 15 IS 14 15
-> IS 15 15 14 12 14
2> 0 0 0 0 1 1
3> 0 TL> 15
0 15
0 IS
11 IS 14
0 15
-> 15 15 15 IS 14 14 2> 0 0 0 0 0 1 TL> 15 15 15 15 14 IS
o00
HWI 6329-100 PRINTED: 16-OCT-91 TABLE.PAGE: 30.1
^8T09
HAZLETON LABORATORIES AMERICA. INC. MADISON, WISCONSIN U.S A.
TABLE 30 INCIDENCE OF SEVERITY OF MICROSCOPIC OBSERVATIONS
S WEEK SACRIFICE
TABLE INCLUDES: SEXALL;GROUP*ALLSCREEN*ALL;WEEKSALL DEATH*1;FIND*ALL;SUBSET-ALL
... N U M B E R - 0 F - A N
SEX:
--MALE---
GROUP: -1- -2- -3- -4- -5- -6-
OROAN/TISSUE EXAMINED
NUMBER: 15 IS 15 IS 14 15
FROM PREVIOUS PAGE
LIVER ILI) ......... .................................. NUMBER EXAMINED: 15 15 15 IS 14 15
--HYPERTROPHY. HEPATOCELLULAR: CHARACTERIZED BY AN ACCENTUATED -> 15 15 3 0 0 15
CENTRILOBULATED PATTERN IN WHICH THE HEPATOCYTES APPEAR TO HAVE 1> 0 0 12 7 S 0
A MORE HOMOGENEOUS CYTOPLASM AND THE CELL BORDERS ARE MORE
2> 0 0 0 I 9 0
ROUNDED GIVING THE CELLS A MORE `PLUMP' APPEARANCE. EXCEPT FO TL> 15 15 IS IS 14 15
THIS `ENLARGED' APPEARANCE. THE CELLS ARE OTHERWISE `NORMAL*.
--HYPERPLASIA,BILE DUCT
-> 15 15 15 15 14 15 TL> 15 15 15 15 14 15
LF. TESTIS (TEO) ...................................... NUMBER EXAMINED: 15 15 15 15 14 15
--ATROPHY/DEGENERATION
> 15 15 15 IS 13 15
3> 0 0 0 0 1 0
TL> 15 15 15 15 14 IS
--INFLAMMATION. GRANULOMATOUS
-> 15 15 15 15 14 15 TL> 15 IS 15 15 14 15
PROSTATE fPR 1 ..................... .................. NUMBER EXAMINED: 15 15 14 15 14 IS
--INFLAMMATION. CHRONIC ACTIVE
-> 15 IS 14 15 14 13
2> 0 3> 0
0 0
0 0
0 0
0 0
1 1
TL> 15 IS 14 15 14 15
SEMINAL VESICLE |SV) .....
.................... NUMBER EXAMINED: 15 15 14 15 14 15
o<o
HWI 6329-100 PRINTED: ll-OCT-91 TABLE.PAGE. 30.2 AFFECTEO---
HAZLETON LABORATORIES AMERICA, INC. MADISON, WISCONSIN U S A .
TABLE 30 INCIDENCE OF SEVERITY OF MICROSCOPIC OBSERVATIONS
5 WEEK SACRIFICE
HWI 6329-100 PRINTED: lt-OCT-91 TABLE.PAGE: 30.3
TABLE INCLUDES: SEX-ALL;GROUP-ALL:SCREEN-ALL;WEEKS-ALL DEATH*1;FIND-ALL;SUBSET*ALL
ORQAN/TISSUE EXAMINED
... N U M B E R - 0 F - A K I M A L S - A F f
SEX: ....
---MALE
GROUP: -1- -2- -3- -4- -5- -a-
NUMBER: 15 15 15 15 14 15
ECTEO-
COAQULATINO OLD (COI ................... ............. NUMBER EXAMINED: IS IS 14 IS 14 is
URETHRA (UR) ........................... ............. NUMBER EXAMINED: IS IS 14 15 14 14
KIDNEY)S) (KD) ......................... ............. NUMBER EXAMINED: 1 2 0 2 3 1
--DILATATION.PELVIC
P> 1 2 0 2 3 1
TL> 1 2 0 2 3 1
SKIN (SK) .............................. ............. NUMBER EXAMINED: 0 0 1 0 0 0
--DERMATITIS
3> 0 0 1 0 0 0
TL> 0 0 1 0 0 0
--ULCERATION
-> 0 0 1 0 0 0 TL> 0 0 1 0 0 0
SPLEEN (SP| ............................ ............ NUMBER EXAMINED: 0 0 0 1 0 0
--CYST, SEROSAL
-> 0 TL> 0
0 0
0 0
1 1
0 0
0 0
MANDIB/CERV LN (MN) !................... --HYPERPLASIA, LYMPHORETICULAR CELL
...... NUMBER EXAMINED: 0 TL> 0
0 0
0 0
0 0
0 0
0 0
MESENTERIC LN (MS) .....................
...... NUMBER EXAMINED: 0 0 0 0 0 0
LUMBAR/ILIAC LN (LM) ................... --HYPERPLASIA. LYMPHORETICULAR CELL
** END OF LIST **
..... NUMBER EXAMINED: 0 0 0 0 0 0 TL> 0 0 0 0 0 0
&U8T00
o
^STGD
HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN US .A .
TABLE 31 INCIDENCE OF SEVERITY OF MICROSCOPIC OBSERVATIONS
WEEK SACRIFICE
TABLE INCLUOES: SX-ALl;CROUP-ALL:SCREEN*ALL;WEEKS*ALL DEATH-2;FIND*ALL:SUBSET-ALL
ORGAN/TISSUE EXAMINED FROM PREVIOUS PAGE * LIVER (LI I ...............
--HYPERPLASIA.BILE DUCT IF. TESTIS (TEO) .......
--A TROPHY/DEGENERATION
--INFLAMMATION. GRANULOMATOUS PROSTATE (PR) ___
--INFLAMMATION. CHRONIC ACTIVE
SEMINAL VESICLE (SV) ___ COAGULATING GLD (CO) ___ URETHRA (UR) ...........
-- N UM B E R -0 F -A N
SEX:
GROUP: -1- -2- -3- -4- -5- -6-
NUMBER: 15- -a15- 15 -aIS-- -m15- -aIS~
NIMBER EXAMINED: IS -> 15
15 15
IS 15
is 15
15 15
15 15
TL> 15 IS 15 15 15 15
NUMBER EXAMINED-:>
15 IS
15 15
15 14
15 15
15 15
IS IS
4> 0 0 1 0 0 0
TL> 15 15 15 15 15 15
-> 15 IS 15 IS 15 15 TL> 15 15 15 15 15 15
NIMBER EXAMINED: 15 -> 14
IS 15
15 15
15 15
15 14
15 IS
2> 1 0 0 0 0 0
3> 0 0 0 0 1 0
TL> IS IS 15 15 15 IS
NUMBER EXAMINED: 15 15 15 15 IS 15
NUMBER EXAMINED: 15 15 15 15 15 IS
NUMBER EXAMINED: 15 IS 15 14 14 14
HWI 6329-100
PRINTED. ll-OCT-91 TABLE.PAGE: 31.2 AFFECTED---
HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U S A .
TABLE 31 INCIDENCE OF SEVERITY OF MICROSCOPIC OBSERVATIONS
I WEEK SACRIFICE
HWI 6329-100 PRINTED: 18-OCT-91 TABLE PAGE: 31.1
TABLE INCLUDES: SXALL;GROUPALL:SCREEN*ALLWEEKS"ALL DEATH-2 FIND-ALL;SUBSET-ALL
---NUMBER-OF-AN IM A L S
SEX:
---MALE---
GROUP: -1- -2- -3- - -5- -6-
ORGAN/TISSUE EXAMINED ** TOP OF LIST BRAIN (BR| .............................
NUMBER: --*15- _B15- --15-- --15 --a15- a15 NUMBER EXAMINED. 15 15 15 15 15 15
LUNG (LU) ..............................
NUMBER EXAMINED: 14 15 13 14 15 IS
LIVER |LI) ............................. --INFILTRATES.MONONUCLEAR CELL
NUMBER EXAMINED: ->
15 11
15 12
15 14
15 11
15 10
15 10
1> 4 3 0 4 3 3
2> 0 0 1 0 2 2
TL> 15 15 15 IS 15 IS
--INF LAMMATION.GRANULOMA TOUS
> 15 IS 15 14 15 IS 1> 0 0 0 1 0 0 TL> IS 15 15 15 15 IS
--NECROSIS.COAGULATIVE
"> 15 15 15 15 14 15 2> 0 0 0 0 1 0 TL> 15 15 15 IS 15 IS
--FATTY CHANGE
-> 15 15 15 15 15 15 TL> 15 IS 15 15 IS 15
--HYPERTROPHY. HEPATOCELLULAR: CHARACTERIZED BY AN ACCENTUATED -> 15 15 3 0 0 IS
CENTRUOBUlAt e D PATTERN IN WHICH THE HEPATOCVTES APPEAR TO HAVE 1> 0 0 12 14 6 0
A MORE HOMOGENEOUS CYTOPLASM AND THE CELL BORDERS ARE MORE
2> 0 0 0 1 9 0
ROUNDED GIVING THE CELLS A MORE 'PLUMP" APPEARANCE. EXCEPT FO TL> 15 15 15 IS IS IS
THIS 'ENLARGED' APPEARANCE, THE CELLS ARE OTHERWISE 'NORMAL' .
** CONTINUED ON NEXT PAGE **
A F F E C T E D ---
C01S7
po
HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U S A .
TABLE 31 INCIDENCE OE SEVERITY OF MICROSCOPIC OBSERVATIONS
I WEEK SACRIFICE
TABLE INCLUDES. SEX-ALL;GROUP-ALL,SCREEN-ALL;WEEKS-ALL DEATH-2;FIND-ALL;SUBSET-ALL
ORGAN/TISSUE EXAMINED
KIDNEY!SI (KDJ .... --DILATATION.PELVIC
SKIN (SKI ..... --DERMATITIS --ULCERATION
SPLEEN ISP) ........................... --CYST. SEROSAL
MANDIB/CERV LN IMN| ................... --HYPERPLASIA, LYMPHORETICULAR CELL
MESENTERIC LN (MS| .................... LUMBAR/ILIAC LN fLM| ..................
--HYPERPLASIA. LYMPHORETICULAR CELL * END OF LIST
-- NUM BER -0 F -A N SEX: GROUP: -1- -2- -3- -4- -5- -6NUMBER: m15 15 -15- -15-- m15 IS NUM8ER EXAMINED: 0 0 1 0 0 0
P> 0 0 1 0 0 0 TL> 0 0 1 0 0 0 NUMBER EXAMINED: 0 0 0 0 0 0 TL> 0 0 0 0 0 0 TL> 0 0 0 0 0 0 NUMBER EXAMINED: 0 0 0 0 0 0 TL> 0 0 0 0 0 0 NUMBER EXAMINED: 0 2 0 1 0 1
3> 0 2 0 1 0 1 TL> 0 2 0 1 0 1 NUMBER EXAMINED: 0 0 0 1 0 0 NUMBER EXAMINED: 0 0 0 0 0 0 TL> 0 0 0 0 0 0
HWI 6329-100 PRINTED: ll-OCT-91 TABLE.PAGE: 31.3
O o
00 <1
fstOO
HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U S A .
TABLE 32 INCIDENCE OF SEVERITY OF MICROSCOPIC OBSERVATIONS
14 WEEK SACRIFICE
TABLE INCLUDES: SEX*ALL;GROUPALL;SCREEN*ALL:WEEKS*ALL DEATH-T,FIND-ALL,SUBSET-ALL
ORGAN/TISSUE EXAMINED TOP OF LIST * BRAIN (BR) ..................................... ..... LUNG (LU) ....................................... ..... LIVER (LI) ...................................... .....
--INFILTRATES.MONONUCLEAR CELL
--INFLAMMATION.GRANULOMATOUS
--NECROSIS,COAGULATIVE
--FATTY CHANGE * CONTINUED ON NEXT PAGE **
__ N U M B E R - 0 F - A N
SEX:
--MALE---
GROUP.- -1- -2- -3- -4- -5- -6-
NUMBER: 15 15 IS 15 15 15
NUMBER EXAMINED: 15 15 15 IS 15 15
NUMBER EXAMINED: 15 15 IS IS 15 15
NUMBER EXAMINED":>
15 12
15 12
15 9
15 10
159
15 7
1> 2 3 5 4 7 6
2> 1 0 1 1 0 2
TL> 15 15 15 15 15 15
- > IS 15 14 12 15 15 1> 0 0 1 2 0 0 2> 0 0 0 1 0 0 TL> 15 15 15 15 15 15
- > 15 14 15 14 15 15 2> 0 1 0 1 0 0 TL> 15 15 IS 15 IS 15
-> 15 15 15 15 15 15 TL> 15 IS 15 15 15 IS
HWI 6329-100 PRINTED: ll-OCT-91 TABLE.PAGE: 32.1
HA2LET0N LABORATORIES AMERICA, INC. MADISON, WISCONSIN U S A .
TABLE 32 INCIDENCE OF SEVERITY OF MICROSCOPIC OBSERVATIONS
14 WEEK SACRIFICE
HWI 6329-100 PRINTED: ll-OCT-91 TABLE.PAGE: 32.2
TABLE INCLUDES: SEXALL;QROUP*ALL.SCREEN-ALL ,WEKSALL DEATH-T;F IND-ALL;SUBSETALL
OROAN/TISSUE EXAMINED * FROM PREVIOUS PAGE LIVER (LII ....
--HYPERTROPHY HEPATOCELLULAR : ROUNDED GIVING THE CELLS A MORE `PLUMP* THIS 'ENLARGED* APPEARANCE. THE CELLS A(
--HYPERPLASIA,BILE DUCT LF. TESTIS (TE01 ........
--ATROPHY/DEGEHERATION
--INFLAMMATION, GRANULOMATOUS
PROSTATE (PR) ...... ............. --INFLAMMATION, CHRONIC ACTIVE
SEMINAL VESICLE (SV|
O O P GO GO*
-- NUMBER-OF-ANIMALS-AFFECTED
SEX:
---MA LE---
GROUP: -1- -2- -3- -4- -5- -6-
NUMBER: -IS-- -IS- -IS 15- mIS- --aIS--
NUMBER EXAMINED: 15 15 15 IS 15 IS
ACCENTUATED -> IS 15 2 1 0 15
APPEAR TO HAVE 1> 0 0 13 12 3 0
ARE MORE
2> 0 0 0 2 12 0
E. EXCEPT FO TL> IS SE "NORMAL".
15
IS
15
15
IS
-> IS 15 IS 15 15 15 TL> IS 15 15 15 15 15
NUMBER EXAMINED-:>
IS IS
15 14
15 15
15 15
IS IS
15 IS
3> 0 1 0 0 0 0
TL> 15 15 15 15 15 15
-> IS 14 15 15 15 15 3> 0 1 0 0 0 0 TL> 15 15 15 15 15 15
NUMBER EXAMINED: ->
IS 15
15 IS
15 13
15 14
IS 14
IS 14
2> 0 0 2 0 0 1
3> 0 0 0 1 1 0
TL> IS 15 15 15 15 15
NUMBER EXAMINED: 15 15 15 15 IS 15
---
HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U.S.A.
TABLE 32 INCIDENCE OF SEVERITY OF MICROSCOPIC OBSERVATIONS
14 WEEK SACRIFICE
HWI 6329-100 PRINTED: It OCT-91 TABLE.PAGE: 32.3
TABLE INCLUDES: SEX-ALL;GROUP-ALLSCREEN-ALL;WEEKS-ALL DEATH-T,FIND-ALL;SUBSET-ALL
ORGAN/TISSUE EXAMINED
COAGULATING OLD (CG| .. URETHRA (UR) ......... KIDNEY]S) IKD) .......
--DILATATION.PELVIC
SKIN --DERMATITIS --ULCERATION
SPLEEN ISP) ...... --CYST, SEROSAL
MANDIB/CERV LN (MN) ................... --HYPERPLASIA. LYMPHORETICULAR CELL
MESENTERIC LN (MS) LUMBAR/ILIAC LN (LM) ..................
--HYPERPLASIA, LYMPHORETICULAR CELL END OF LIST **
__ N U M B E R - 0 F - A N I M A L S - A F F E C T SEX: GROUP: -1- -2- -3- -4- -5- -8NUMBER: -15- -1a5m --15-- -1m5-- IS -mIS-
... NUMBER EXAMINED: 15 15 15 15 15 is ... NUMBER EXAMINED: 15 is 15 IS IS 15 ... NUMBER EXAMINED: 0 0 0 1 1 0
P> 0 0 0 1 1 0 TL> 0 0 0 1 1 0 ... NUMBER EXAMINED: 0 0 0 0 0 0 TL> 0 0 0 0 0 0 TL> 0 0 0 0 0 0 ... NUMBER EXAMINED: 0 0 0 0 0 0 TL> 0 0 0 0 0 0 ... NUMBER EXAMINED: 1 0 0 0 1 0
3> 1 0 0 0 1 0 TL> 1 0 0 0 1 0 ... NUMBER EXAMINED: 0 0 0 0 0 0 ... NUMBER EXAMINED: 0 0 0 0 0 0 TL> 0 0 0 0 0 0
E D ---
fsSTOO
O
HAZLETON LABORATORIES AMERICA, INC. MADISON, WISCONSIN U S A.
TABLE 33 INCIDENCE OF SEVERITY OF MICROSCOPIC OBSERVATIONS
22 WEEK SACRIFICE
HWI 6329-100 PRINTED: 18-OCT-91 TABLE.PAGE : 33.1
TABLE INCLUDES: SEXALL;GROUP*ALLSCREEN*ALL ;WEEKSALL DEATH.U;FIND-ALL ;SUBSET-ALL
ORQAN/TISSUE EXAMINED * TOP OF LIST BRAIN (BRI ....... ........................ ......... LUNG (LU| ...... .......................... ......... LIVER ( H I ................................. .........
--INFILTRATES,MONONUCLEAR CELL
--INFLAMMATION.GRANULOMATOUS
--NECROSIS.COAGULATIVE
--FATTY CHANGE
,
CONTINUED ON NEXT PAGE *
-- N UM B E R -0 F -A N IM A LS
SEX:
--MALE---
GROUP : -1- -2- -3- -4- -5- -6-
NUMBER: 10 10 10 10 10 0
NUMBER EXAMINED: 10 10 9 10 10 NUMBER EXAMINED: 10 10 10 10 10 NUMBER EXAMINED: 10 10 10 10 10
-> 1 6 1 9 7 1> 1 3 1 1 1 2> 1 1 1 0 2 TL> 10 10 10 10 10 -> 1 10 10 10 10 1> 1 0 0 0 0 2> 1 0 0 0 0 TL> 10 10 10 10 10 -> 9 9 9 9 10 2> 1 1 1 1 0 TL> 10 10 10 10 10 -> 10 10 9 10 10 3> 0 0 1 0 0 TL> 10 10 10 10 10
0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
A F F E C T E D ---
jf&STOO
HAZLETON LABORATORIES AMERICA. INC. MADISON. WISCONSIN U S A .
TABLE 33 INCIDENCE OF SEVERITY OF MICROSCOPIC OBSERVATIONS
22 WEEK SACRIFICE
TABLE INCLUDES: SEX'ALL:GROUPALL:SCREENALL;WEEKS"ALL DEATH>U.FINDALL:SUBSET-ALL
-- N UM B E R -0 F -A N
SEX:
--MALE---
GROUP: -1- -2- -3- -4- -5- -6-
ORQAN/TISSUE EXAMINED --------------------------
------- --------
---------- ----N--U--MBER: --10-- -10- 10 ~10-- 10- -0"
** FROM PREVIOUS PAGE LIVER (LI) ........ .................................... NUMBER EXAMINED: 10 10 10 10 10
--HYPERTROPHY. HEPATOCELLULAR: CHARACTERIZED BY AN ACCENTUATED -> 10 10 10 10 10 CENTRILOBULATED PATTERN IN WHICH THE HEPATOCYTES APPEAR TO HAV TL> 10 10 10 10 10
0 0 0
A MORE HOMOGENEOUS CYTOPLASM AND THE CELL BORDERS ARE MORE
ROUNDED GIVING THE CELLS A MORE `PLUMP' APPEARANCE. EXCEPT FOR
THIS `ENLARGED* APPEARANCE, THE CELLS ARE OTHERWISE `NORMAL*.
--HYPERPLASIA.BILE DUCT
2-,>
10 0
9 10 10 10 100 0
TL> 10 10 10 10 10
0 0 0
LF. TESTIS (TEO) ...................................... NUMBER EXAMINED: 10 10 10 10 10
--ATROPHY/DEGENERATION
-> 10 9 9 9 10
2> 0 1 0 0 0
3> 0 0 1 1 0
TL> 10 10 10 10 10
0 0 0 0 0
--INFLAFWATION, GRANULOMATOUS
-> 10 10 10 10 10 TL> 10 10 10 10 10
0 0
PROSTATE (PRI ...................... , --INFLAMMATION, CHRONIC ACTIVE
........... NUMBER EXAMINED: 10 10 10 10 10 -> I 10 10 10 1 2> 2 0 0 0 1 5> 0 0 0 0 1
TL> 10 10 10 10 10
0 0 0 0 0
HWI 6329-100
PRINTED: II -OCT-91 TABLE.PAGE: 33.2 A F F E C T E D ---
S8TG0
oo
/sSTOO
HAZLETON LABORATORIES AMERICA, INC. MADISON. WISCONSIN U S A .
TABLE 33 INCIDENCE OF SEVERITY OF MICROSCOPIC OBSERVATIONS
22 WEEK SACRIFICE
TABLE INCLUDES: SEX-ALL;GROUP-ALL;SCREEN-ALL;WEEKS-ALL DEATH-U:FIND-ALL;SUBSE TALL
ORGAN/TISSUE EXAMINED
N UM B E R -0 F -A N
SEX:
--MALE
GROUP: -1- -2- -3- -4- -5- -6-
NUMBER: 10m -10- 10 10-- -10- *0m
SEMINAL VESICLE | S V ) .................... ............. NUMBER EXAMINED: 10 10 10 10 10 0
COAGULATING OLD ICG) .................... ............ NUMBER EXAMINED: 10 10 10 10 10
URETHRA (UR) ............................ ............. NUMBER EXAMINED: 10 10 10 10 10
KIDNEY(S) (KD) .......................... ............ NUMBER EXAMINED: 0 0 0 1 0
--DILATATION,PELVIC
P> 0 0 0 1 0
TL> 0 0 0 1 0
SKIN (SK| ............................... ............ NUMBER EXAMINED: 1 0 0 0 0
--DERMATITIS
5> 1 0 0 0 0
TL> 1 0 0 0 0
--ULCERATION
5> 1 0 0 0 0 TL> 1 0 0 0 0
0 0 0 0 0 0 0 0 0 0
SPLEEN (SP) ............................. ............. NUMBER EXAMINED: 1
--CYST, SEROSAL
P>
0 0
0 0
0 0
0 0
0 0
TL> 1 0 0 0 0 0
MAXDIB/CERV LN (MN) ..................... --HYPERPLASIA, LYMPHORETICULAR CELL
.... NUMBER EXAMINED: 0 0 0 0 1 0 3> 0 0 0 0 1 0
TL> 0 0 0 0 1 0
MESENTERIC LN (MS) ......................
.... NUMBER EXAMINED: 0 0 0 0 0 0
o
HWI 6329-100 PRINTED: 11-OCT-R1 TABLE.PAGE: 33.3
HWI 6329-100
Table 34 Summary of Hormone Data
Week
5 Mean SD N
8 Mean SD N
14 Mean SD N
22 Mean SD N
0
20.1
-
1
22.6
-
1
b
-
-
b
"
T-5180 fpom) 1 10 30
Estradiol (oa/mtl
21.0 0.42 2
b
-
-
26.8 4.56 4
b
34.2
29.1
- - 6.99
16
b
20.9
26.7
-
1.16
4.17
-^ 3
2
28.9 5.23 3
25.3 3.70 5
25.3 7.10
3
100
33.7 9.93 10
36.6 10.43
5
28.2 3.69 4
25.9 2.22 3
0*
21.3 1.70 2
20.5 0.12 3
b
-
NA NA NA
Testosterone fna/ml)
5
Mean
72,400
66,222
59,667
49,200
31,600
42,100
SD
55,580.2
84,812.7
31,815.9
23,403.7 23,735.6
32,370.9
N 10 9 9 10 10 10
8
Mean
46,562
49,913
35,984
73,790
55,680
34,410
SD
39,398.1
66,500.6
22,768.7 103,947.1 35,655.4
38,889.9
N
10 10 10 10 10
10
^SSTOO
^ a Animal were pair-fed with the high-dose animals throughout treatment, g b Mean is less than 20.0 pg/mL.
HW1 6329-100
Table 34 (Continued) Summary of Hormone Oata
Week
14 Mean SO N
22 Mean SD N
0
24,578 12,656.3
10
8,714 2,517.6
5
T-5180 (ppm)
1
_10_
30
Testosterone (na/mL)
35,649 27,614.6
10
31,478 21,909.0
10
23,577 19,074.1
10
21,680 5,669.8 5
11,946 7,885.8 5
13,660 5,981.4 5
100
20,413 14,625.6
10
35,864 38,519.5
5
0*
19,462 16,005.'
10
NA NA NA
5 Mean SD N
8 Mean SD N
14 Mean SD N
22 Mean SD N
0.5 0.19 10
0.5 0.12 9
0.4 0.07 9
0.4 0.17 5
Luteinizing (nq/rnL)
0.7 0.28 10
0.6 0.16
10
0.6 0.19 10
0.6 0.16 9
0.5 0.09 8
0.5 0.14 9
0.5 0.10 10
0.4 0.14 10
0.5 0.19 9
0.4 0.05 4
0.5 0.12 5
0.5 0.32 5
0.5 0.12 8
0.5 0.12 10
0.6 0.45 10
0.6 0.22 4
0.6 0.20 10
0.5 0.20 9
0.4 0.13 10
NA NA NA
^ISSTOO
a Animals were pair-fed with the high-dose animals throughout treatment.
A -. HWI 6329-100
Table 35
Summary of Test Material Residue Analysis Males
Wek 5 8
14 22
Mean SD N
Mean SD N
Mean SD N
Mean SD N
_L
a a a
a a a
a a a
a a a
_2_
6.5 1.05 8
7.5 1.27 9
7.1 1.15 10
1.2
-
1
T-5180 (ppml
group 34
55.4 8.08 9
103.5 14.38 8
45.5 16.20 10
87.3 27.48 10
41.2 12.98 10
70.3 16.18 10
1.09 1.303 3
1.64 0.918 3
159.3 30.16 10
149.1 34.98 10
137.6 33.83 10
2.45 0.849 2
6
a a a
a a a
a a a
.
-
-
a All values were below the limit of detection.
ooiss
HWI 6329-100
Table 36
Results of Method Validation for Determination of T-5180 in Rodent Food Expressed as the Percent of Recovery
Theoretical
__ L(oeovrecll
1 1 1
10 10 10
30 30 30
100 100 100
Analytical
_As_s_ayiSRMesl_u_lt_s
Pay 1
0.810
1.08 0.760
10.4
9.92 9.20
Mean SD CV (%)
N
30.1 34.2 30.6
Mean
SO CV {%) N
Mean
SD CV (%) N
99.4 95.0 102
Mean SD CV (%) N
Percent Theoretical
i%I,_ .
81.0 108
76.0
88.3 17.20 19.50
3
104 99.2 92.0
98.4 6.00 6.10 3
100 114 102
105 7.50 7.10 3
99.4 95.0 102
98.8 3.50 3.60 3
123
< 0i 8 8 f
HWI 6329-100
Table 36 (Continued)
Results of Method Validation for Determination of T-5180 in Rodent Food Expressed as the Percent of Recovery (Continued)
Theoretical Level
___ Lsml___
1.000 1.000 1.000
Analytical Assay Results ____ ififisQ____
Dav 1 (Continued!
977 972 1,072
Mean SD CV (X) N
Percent Theoretical ____ m ____
97.7 97.2 107
101 5.60 5.60 3
Pay-.?
1 -0.082
1 0.627
1 0.924
r 1.24
r l*
01..29095
Mean
SD
CV (X)
N
111000
10.6
9.85 9.66
Mean
SD CV (X) N
a Data are from reanalysis of sample.
62.7 92.4 124 99.5 120
99.7 24.60 24.7
5
106 98.5 96.6
100 5.00 5.00 3
124
0018^9
HWI 6329-100
Table 36 (Continued)
Results of Method Validation for Determination of T-5180 in Rodent Food Expressed as the Percent of Recovery (Continued)
Theoretical Level
___ (ppm)___
30 30 30
100 100 100
1,000 1,000 1,000
Analytical Assay Results ____ (ppm)____
Day 2 (Continued)
28.8 28.0 28.3
Mean - SD
CV (X) N
97.1 93.3 94.3
Mean SD CV (X) N
1,006 955 940
Mean SD CV (X) N
Percent Theoretical
(%)
96.0 93.3 94.3
94.6 1.30 1.40 3
97.1 93.3 94.3
94.9 2.00 2.10 3
101 95.5 94.0
96.8 3.70 3.80 3
125
00189
HWI 6329-100
Table 37 Results of Stability Analyses
Storage Conditions Day of mixing,
12/19/90
Refrigerated, 1 day; room temperature, 7 days
Frozen, 2 weeks
Frozen, 7 weeks
ReDlicate
1 2 Mean
1 2 Mean
1 2 Mean
1 2 Mean
_________________________ T-5180 (ppm)
01
10
- 1.27 - 1.39 - 1.33 (133)'
10.0 8.90 9.45 (94.5)
- 0.81 - 0.75 - 0.78 (78.0)
11.8 10.8 11.3 (113)
0.75 - 0.78 - 0.77 (77.0)
_ 1.20 - 1.47 - 1.34 (134)
10.2 12.2 11.2 (112)
11.1 9.44 10.3 (103)
a Values in parentheses are percent of theoretical.
30
28.3 27.7 28.0 (93.3)
28.9 33.7 31.3 (104)
28.7 29.5 29.1 (97.0)
26.6 26.6 26.6 (88.7)
100
93.2 90.3 91.8 (91.8)
109 108 109 (109)
109 112 111 (111)
107 109 108 (108)
O o>
Q0 JS>
HWI 6329-100
Table 37 (Continued) Results of Stability Analyses
Storage Conditions*
Day of mixing, 03/06/91
Room temperature, 8 days
ReDlicate
1 2 Mean
1 2 Mean
_________________________ T-5180 (ppm)
01
10
- 0.68
9.09
- 0.76
9.62
-
0.72 (72.0)*
9.36 (93.6)
- 0.74 - 0.76 ** 0.75 (75.0)
9.40
9.72 9.56 (95.6)
30
28.9 29.7 29.3 (97.7)
29.5 29.7 29.6 (98.7)
100
96.7 94.0 95.4 (95.4)
94.1 94.6 94.4 (94.4)
a Storage condition reflect actual conditions that samples were stored from Weeks 1 through 8. b Values in parentheses are percent of theoretical.
*<6ST00
t-oo
HW I 6329-100
Table 38 Results of Homogeneity Analyses
Sample Location Top
Left
Right ,
Bottom
ReDlicate
1 2
1 2
1 2
1 2
_________________________ T-5180 (ppm)
01
10
-
0.72 (72.0)*
11.6 (116)
0.65 (65.0)
12.0 (120)
- 0.95 (95.0) 10.7 (107) - 0.88 (88.0) 10.9 (109)
- 1.21 (121) - 1.54 (154)
12.8 (128) 11.6 (116)
- 0.85 (85.0) 11.9 (119) - 0.84 (84.0) 12.7 (127)
30
31.3 (104) 29.8 (99.3)
29.4 (98.0) 31.1 (104)
34.2 (114) 30.5 (102)
28.7 (95.6) 31.0 (103)
a Values in parentheses are percent of theoretical.
100
104 (104) 106 (106)
114 (114) 119 (119)
107 (107) 106 (106)
106 (106) 110 (110)
rv>
Of) 00
HWI 6329-100
Table 38 (Continued) Results of Homogeneity Analyses"
Sample Location Top
Left
Right
Bottom
Reolicate
1 2
1 2
1 2
1 2
_________________________ T-5180 (ppm)
01
10
0.84 (84.0)b 0.88 (88.0)
0.88 (88.0) 0.86 (86.0)
0.73 (73.0)c 0.74 (74.0)'
0.71 (71.0) 0.87 (87.0)
30
-
-
"
a Reanalyzed, 01/07/91. b Values in parentheses are percent of theoretical. c Results are from a reanalysis on 1/15/91.
100
-
-
-
* -
-
* -
/^68T00
NlO
HUI 6329-100
Table 39 Results of Dose Preparation Analyses
Week
R e d icate
0
T-5180 (ppm) 10
30
1
1
-
0.80 (80.0)"
11.6 (116)
28.9 (96.3)
2
- 1.19 (119)
11.4 (114)
29.5 (98.3)
2
1
- 0.80 (80.0)
9.73 (97.3)
32.2 (107)
2
- 0.91 (91.0) 10.8 (108)
33.0 (110)
31 ,2
41 2
0.91 (91.0) 10.1 (101) - 0.74 (74.0) 11.5 (115)
34.9 (116) 31.4 (105)
_ 0.73 (73.0) 11.5 (115)
30.4 (101)
- 0.74 (74.0)
9.88 (98.8)
30.6 (102)
4b 1 2
_ 0.88 (88.0) - 0.82 (82.0)
5 1 - 0.86 (86.0) 2 - 0.88 (88.0)
5b 1 2
- 1.10 ( H O) - 1.17 (117)
-
6 1 _. 2 --
9.58 (95.8) 10.4 (104)
-
a Values In parentheses are percent of theoretical, b Archived sample.
100 111 (111) 102 (102) 100 (100) 108 (108) 98.4 (98.4) 110 (110) 98.0 (98.0) 102 (102)
-
-
OO)
jifeSTOd
HWI 6329-100
Table 39 (Continued) Results of Dose Preparation Analyses
Week
Replicate
0
1
T-5180 iDPml _I0_
30
7 1 -2-
- 29.1 (97.0)' - 26.1 (87.0)
8 1 -2 -"
--
9
1
- 0.70 (70.0)
2
- 0.75 (75.0)
-
_
10 1 - 2-
10.3 (103) 9.01 (90.1)
-
11 1 - 2-
- 27.7 (92.3) - 28.8 (96.0)
12 1 - 2"
--
--
13
1
- 0.76 (76.0)
-
2
- 0.72 (72.0)
-
-
-
14 1 - 2 --
9.34 (93.4) 9.45 (94.5)
_
-
a Values in parentheses are percent of theoretical.
100
-
97.3 (97.3) 96.6 (96.6)
_
-
-
-
90.1 (90.1) 93.2 (93.2)
_
-
-
^68TGD
HW I 6329-100
APPENDIX A Protocol Deviations
Protocol TP9321 Protocol Amendment No. 1 Protocol Amendment No. 2 Protocol Amendment No. 3 Material Safety Data Sheet
132
G01S9J
HWI 6329-100
Protocol Deviations
Protocol
Dose Preparation. "All dose preparations will be refrigerated until administration.*
Dose Analyses. "Four additional sets of samples will be taken from each dose preparation mixed for Week 1...0ne set will be kept for at least the same period and under the same conditions that are expected to be used for the study, then analyzed."
Special Analysis. Test Material Residue Analysis. "An aliquot of serum ...will be collected and stored until analysis for test material residue."
Special Analysis. Disposition. "Serum samples will be stored at approximately -70'C until shipment."
Termination. "Animals will be anesthetized with methoxyflurane, exsanguinated, and necropsied between 7:00 a.m. and 10:00 a.m."
Gross Necropsy. Cut surfaces of the brain and spinal cord will be examined when tissues are trimmed.
Actual Procedure
During Weeks 1 through 8, diets were stored at room temperature until administration.
None of the sets of samples mixed for Week 1 were stored under the same conditions as was used for Weeks 1 through 8, so a second stability analysis was done at Week 12.
All serum samples from the Week 22 collection were sent to Hazleton Washington for hormone analysis. The serum remaining after hormone analysis was returned to Hazleton Wisconsin for test material residue analysis. Due to an Insufficient amount of serum remaining after hormone analysis, some groups had serum from less than 10 animals available for test material residue analysis.
The location of serum sample storage for 3 collection dates was not documented and cannot be verified.
Two animals were not weighed before exsangulnatlon. Three animals were necropsied after 10:00 a.m.
The spinal cord was not collected at necropsy; therefore, cut surfaces of the spinal cord were not examined when tissues were trimmed.
These deviations are not expected to have affected the results of the study.
133
HAZLETON
WISCONSIN
330* KINSMAN B O U L E v AAO POST O F F IC E BOX 754$ MADISON WISCONSIN S3707
O O B N M Q tAOc^nyy Sendees C o ^ -.
Sponsor: 3M
St. Paul, Minnesota
PROTOCOL TP9321
Study Title:
13-Week Dietary Toxicity Study with T-5180, Ammonium Perfluorooctanoate (CAS No. 3825-26-1) in Male Rats
Date: November 30, 1990
Performing Laboratory: Hazleton Wisconsin, Inc.
3301 Kinsman Boulevard Madison, Wisconsin 53704
Laboratory Project Identification: HW1 6329-100
\ j r . > e <J $ 1 I 1 t > G t f ?T> ft y J A P t n
. P r > n C U 1 t t 3
** C -Q --
P n o r e E O S - 2 4 1 ' 4 ^ 1 F u t O i - * ' -7 22 J T e 7 0 3 9 5 6 m a Z P A w V O S
134
coi^<J9
HWI 6329-100 TP9321 Page 2
STUDY IDENTIFICATION
13-Week Oietary Toxicity Study with T-5180, Ammonium Periluorooctanoate (CAS No. 382S-26-1) in Hale Rats
Test Material Sponsor
Study Monitor
Study Director
Study Location
Proposed Study Timetable In-Life (Experimental) Start Date In-Life Termination Date Experimental Termination Date
T-5180, Ammonium Perfluorooctanoate (CAS No. 3825-26-1)
3M Toxicology Services Building 220-2E-02, 3M Center St. Paul, Minnesota 55144-1000
Roger G. Perkins, PhO, DABT 3M Toxicology Services Building 220-2E-02, 3M Center St. Paul, Minnesota 55144-1000
Matthew J. Palazzolo, PhD, DABT Hazleton Wisconsin, Inc. P.0. Box 7545 Madison, Wisconsin 53707 (608) 242-2622
Hazleton Wisconsin, Inc. 3301 Kinsman Boulevard Madison, Wisconsin 53704
December 20, 1990 May 16, 1991 To be provided by amendment
135
CO19O0
1. Study 2. Purposes
3. Regulatory Compliance
4. Quality Assurance
5. Test Material A. Identification B. Purity C. Stability D. Storage Conditions E. Characteristics
F. Reserve Samples
G. Safety Precautions
HWI 6329-100 TP9321 Page 3
13-Week Oietary Toxicity Study with T5180, Ammonium Perfluorooctanoate (CAS No. 3825-26-1) in Male Rats
To assess the subchronic toxicity of the test material when fed to rats for at least 13 weeks and to evaluate reversibility of any toxic effects
This study will be conducted in accordance with the Environmental Protection Agency Good Laboratory Practice Standards, 40 CFR 160.
The protocol, study conduct, and final report will be audited by the Quality Assurance Unit in accordance with Hazleton Wisconsin, Inc. (HW1) Standard Operating Procedures (SOPs).
T-5180, Ammonium Perfluorooctanoate (CAS No. 3825-26-1)
To be determined by the Sponsor
To be determined by the Sponsor
In a dry area, at room temperature
Information on synthesis methods, composition, or other characteristics that define the test material is on file with the Sponsor.
A reserve sample of each lot will be taken and stored in a freezer. These samples will be transferred to the Sponsor after completion of the inlife phase of the study to be retained in accordance with 40 CFR 160.195. Any remaining test material will be returned to the Sponsor after their authorization.
As required by HWI SOPs, unless otherwise specified
136
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HWI 6329-100 TP9321
Page 4
6. Experimental Oesign A. Animals (1) Species (2) Straln/Source (3) Age at Initiation of Treatment (4) Weight at Initiation of Treatment (5) Number and Sex (6) Identification (7) Husbandry (a) Housing (b) Food
(c) Water
(d) Contaminants
(e) Environment
(f) Acclimation
Rat
Crl:CD*BR/Charles River Laboratories, Inc.
Preferably 4 to 6 weeks, but not older than 8 weeks
Approximately 75 to 250 g
320 males
Individually numbered ear tags
Individual
Certified Rodent Chow #5002 (Purina Hills, Inc.) d libitum, unless otherwise specified. The food is routinely analyzed by the manufacturer for nutritional components and environmental contaminants.
Ad libitum. Samples of the water are analyzed by HWI for total dissolved solids, hardness, and specified microbiological content and for selected elements, heavy metals, organophosphates, and chlorinated hydrocarbons.
There are no known contaminants in the food or water that would interfere with this study.
Environmental controls for the animal room will be set to maintain a temperature of 72*F 6*. a relative humidity of 50% 20%, and a 12-hour 1ight/12-hour dark cycle.
At least 1 week
137
0013 OpLi'
HWI 6329-100 TP9321 Page 5
(8) Randomization
Via computer-generated random numbers for assignment to groups. The animals will be weighed. The weight variation of the animals selected for the study will not exceed 2 standard deviations of the mean weight, and the mean body
weights for each group will not be statistically different at the
5* probability level.
(9) Justification
The rat is frequently used in safety evaluation studies as a representative of a rodent species. Hale rats are used to characterize the effects of the test material on testicular physiology.
Group Designations
Group
Dose Level (ppml
Number of Males
1 0 55 2 1 55 3 10 55 4 30 55
5 100 55 6 0b 45
a 10 animals for Groups 1 through 5 (designated as recovery animals) will be treated for at least 13 weeks, then treatment will be discontinued and they will be observed for reversibility, persistence, or delayed occurrence of toxic effects for at least 8 weeks posttreatment,
b Animals in Group 6 will be pair-fed with the high-dose animals (Group S) throughout the treatment period. Oue to the pair-feeding requirements, the pair-fed control animals (Group 6) will initiate on test 1 day after all other groups.
C. Dosing Procedures
(1) Dosing Route
Dietary
(2) Reason for Dosing Route
To compare the test data with other studies dosed in a similar fashion.
(3) Dosing Duration
At least 13 weeks
138
coi9o:
HWI 6329-100 TP9321
Page 6
(4) Oose Preparation
(5) Oose Analyses (a) Homogeneity
(b) Stability
(c) Routine Analyses
D. Observation of Animals (1) Cageside Observations (2) Antemortem Observations
Weekly. All diets will be mixed according to the mixing procedure. Dose concentrations will be based on the test material as supplied. All dose preparations will be refrigerated until being dispensed into food containers.
By HWI. Additional samples may be assayed at the discretion of the Study Director.
One sample each from all dose levels mixed for Week 1 will be taken from the top, bottom, and two opposing sides of the dose preparation and assayed for test material content. All samples will be stored in a freezer until analyzed.
Four additional sets of samples will be taken from each dose preparation mixed for Week 1. One set will be analyzed on the day of mixing. One set will be kept for at least the same period of time and under the same conditions that are expected to be used for the study, then analyzed. The remaining two sets will be stored in a freezer and assayed after 2 and ? weeks.
All dose preparations will be assayed each week for the first 4 weeks. Each week thereafter, one test material dose concentration will be selected sequentially. Samples from each 4week period will be analyzed as a group with a sample of the control diet.
Mortality and morlbundity check twice dally (a.m. and p.m.). Additional signs of poor health or abnormal behavior will be recorded as they are observed.
At least once weekly, each animal will be removed from its cage and examined.
139
(3) Body Weights (4) Food Consumptions E. Special Analysis (1) Hormone Analysis
(a) Method of Collection
(b) Disposition
HWI 6329-100 TP9321 Page 7
Before initiation of treatment, on the first day of treatment, weekly thereafter, and on the day of necropsy
Daily for the animals that will be pair-fed (Groups 5 and 6); weekly for all other animals. During recovery, Group 5 animals will be fed weekly.
Serum samples will be collected from animals scheduled for sacrifice at each interval for analysis of estradiol, testosterone (total), and luteinizing hormone. Serum samples from 10 animals/group selected at random at each scheduled sacrifice will be analyzed from each sacrifice interval during treatment. Serum samples from 5 animals/group will be analyzed at recovery. The following methods will be used for the hormone analysis:
. luteinizing hormone radioimmunoassay
Estradiol - Diagnostic Products Kit (DPCKE2D1)
Testosterone - Diagnostic Products Kit (DPCTKTTZ)
Nonfasted animals will be anesthetized with methoxyflurane; blood will be collected from the descending vena cava and stored on ice (approximately 4*C) until centrifugation.
Serum samples will be stored at approximately -70*C until shipment. All samples will be shipped on dry ice to:
Denise Novello Immunochemistry Oivision Client Services Section Hazleton Washington 9200 Leesburg Turnpike Vienna, Virginia 22182
140
coiaoif
HWI 6329-100 TP9321 Page 8
(2) Test Material Residue Analysis
(3) Hepatic Palmitoyl CoA Oxidase
F. Termination (1) Unscheduled Sacrifices and Deaths (2) Scheduled Sacrifice
G. Postmortem Procedures (1) Gross Necropsy
Samples of liver, testes, lungs, and subcutaneous adipose from each animal at necropsy will be collected and stored frozen for possible analysis for test material residue (at an additional cost to the Sponsor; method to be provided by the Sponsor). An aliquot of serum (see Section 6. E. 1. Hormone Analysis) will be collected and stored for possible analysis for test material residue.
A section of liver will be obtained from all animals at each scheduled sacrifice and will be flash frozen using liquid nitrogen. A section of liver from S animals/group, selected at random at each scheduled sacrifice will be assayed for the level of palmitoyl CoA oxidase as an indicator of peroxisome proliferation.
Necropsies will be done on all animals that die or are sacrificed in a moribund condition.
After at least 4, 7, and 13 weeks of treatment, animals (15/group) will be anesthetized with methoxyflurane, weighed, exsanguinated, and necropsied between 7:00 a.m. and 10:00 a.m. For recovery animals (10/group), this will be done after at least 13 weeks of treatment and at least 8 weeks without treatment.
The necropsy will include an examination of:
External surface of the body All orifices Cranial cavity External surfaces of the brain and
spinal cord (Cut surfaces will be examined when tissues are trimmed.)
141 C-01900
(2) Organ Weights
(3) Special Analyses (4) Tissue Preservation
HWI 6329-100 TP9321 Page 9
Nasal cavity and paranasal sinuses Thoracic, abdominal, and pelvic
cavities and viscera
For each animal at scheduled sacrifice, the following organs will be weighed; paired organs will be weighed separately:
Brain Liver Lungs Testes Accessory sex organs (seminal
vesicle, prostate, coagulating gland, urethra)
Organ-to-body weight percentages and organ-to*brain weight ratios will be calculated.
Samples of liver, testes, lungs, and subcutaneous adipose will be collected from each animal for possible analysis for test material residue. An additional section of liver will be 'collected from each animal for possible analysis of Palmltoyl CoA. (See Section 6. E. 3. Hepatic Palmltoyl CoA Oxidase).
The following or representative samples thereof, will be collected and preserved In 10% phosphate-buffered formalin, unless otherwise specified. Samples of these tissues will be preserved in glutaraldehyde for further processing 1f electron microscopic evaluation is desired.
Lesions Brain Liver Lungs Testes Accessory sex organs (seminal
vesicle, prostate, coagulating gland, urethra)
142
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HWI 6329-100
TP9321 Page 10
(5) Hlstopathology
7. Reports A. Final Report
(1) Experimental Design and Methods
(2) Results
(3) Statistical Analyses
The above (as appropriate) will be embedded 1n paraffin, sectioned, stained with hematoxylin and eosin, and examined microscopically from all animals from which they are collected
A final report including, but not limited to, those items listed below will be submitted. One copy of the draft report and two copies of the final report will be provided.
As defined by the protocol, protocol amendments, and any protocol deviations
Mortality Antemortem observations Body weights Cumulative body weight gains Food consumption Compound consumption Palmitoyl CoA Organ weights Organ-to-body weight percentages Organ-to-brain weight ratios Macroscopic observations Microscopic observations
Body weights Cumulative body weight gains Food consumption Organ weights Organ-to-body weight percentages Organ-to-brain weight ratios Palmitoyl CoA Hormone Analysis
Groups 2, 3, 4, and 5 will be compared with Group 1; Group 6 will be compared with Group 5. Statistical methods will be those used by HWI (Attachment 1).
143
coiaotf
HWI 6329-100
TP9321 Page 11
(4) Data to be reported
(5) Analytical Chemistry 8. Maintenance of Raw Data,
Records, and Specimens
Incidence of antemortem observations Mean body weights Mean cumulative body weight gains Mean food consumptions Mean compound consumptions Mean group hormone values Mean Palmitoyl CoA Mean organ weights Mean organ-to-body weight percentages Mean organ-to-brain weight ratios Incidence of macroscopic observations Incidence of microscopic observations
Individual antemortem observations (to include fate and date of death for each animal)
Individual body weights Individual food consumptions Individual Palmitoyl CoA values Individual organ weights Individual organ-to-body weight
percentages Individual organ-to-brain weight ratios Individual macroscopic observations Individual microscopic observations
Methods and results (if done by HVI)
Original data, or copies thereof, will be available at HWI to facilitate auditing the study during its progress and before acceptance of the final report. When the final report is completed, all original paper data, all magnetically encoded records, and a copy of the final report will be retained in the archives of HWI. All specimens will be transferred to the Sponsor to be retained in accordance with 40 CFR 160.195.
144 0190^
PROTOCOL APPROVAL
HWI 6329-100 TP9321 Page 12
Aatthar J~ PtUzzolo,
Study Director
Toxicology Hazleton Wisconsin, Inc.
OABT
Representative Quality Assurance Unit Hazleton Wisconsin, Inc.
Date Dat /
RECEIVED
DEC 0 6B90
HLA-Madison
145
001310
HWI 6329-100 TP9321 Page 13
Attachment 1
Statistical Analyses
The statistical methods that will be used are described below, levene's test (Levene, 1960) will be done to test for variance homogeneity. In the case of heterogeneity of variance at p s 0.05, transformations will be used to stabilize the variance.
Analysis of variance (AN0VA) (Winer, 1971) will be done on the homogeneous or transformed data. If the ANOVA is significant, Games and Howell Modified TukeyKramer test (Games and Howell, 1976) will be used for pairwise comparisons between groups.
One-way ANOVA will be used (if applicable) to analyze body weights; cumulative body weight gains; food consumptions; Palmitoyl CoA and Hormone Analysis; organ weights; organ-to-body weight percentages; and organ-to-brain weight ratios.
If the ANOVA shows significance for body weights at Week 0, one-way analysis of covariance (ANCOVA) (Winer, 1971) will be used to analyze body weights, with the initial body weight as the covariate. Although Levene's test for variance homogeneity will be done (see above), no transformations will be used because covariance adjustment removes extraneous heterogeneity. If the ANCOVA is significant, Games and Howell Modified Tukey-Kramer test will be used for pairwise comparisons between groups.
Group comparisons will be evaluated at the 5.0% two-tailed probability level.
References
Games, P. A., and Howell, J. F., "Pairwise Multiple Comparison Procedures with Unequal N's and/or Variances: A Monte Carlo Study," Journal of Educational Statistics. 1(2): 113-125 (1976).
Levene, H., "Robust Tests for Equality of Variances," Contributions to Probability and Statistics, (eds.) I. Olkin et al., Ch. 25, pp. 278-292, Stanford University Press: Stanford, California (1960).
Winer, 8. J., "Analysis of Covariance," Statistical Principles in Experimental Desion. Second Ed., Ch. 10, pp. 752-812, McGraw-Hill: New York, New York (1971).
Winer, B. J., "Design and Analysis of Single-Factor Experiments," Statistical Principles in Experimental Desion. Second Ed., Ch. 3, pp. 149-260, McGraw-Hill: New York, New York (1971).
146
CQISi/
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v - o S O N .V s c o n s .>. are-
HWI 6329-100
.o o m n n o
-T- *
PROTOCOL TP9321
13-Week Dietary Toxicity Study with T-5180, Ammonium Perfluorooctanoate (CAS No. 3825-26-1) in Hale Rats
HWI 6329-100
Sponsor
3M Toxicology Services Building 220-2E-02, 3H Center St. Paul, HN 55144-1000
Study Monitor
Roger 6. Perkins, PhD, DABT
Contractor
Hazleton Wisconsin, Inc. 3301 Kinsman Boulevard Madison, WI 53704
Study Director
Matthew J. Palazzolo, PhD, DABT
Amendment N. 1-
This amendment modifies the following portions of the protocol:
Effective December 10. 1990 1 Page 6, 6. Experimental Design, C. Dosino Procedures. 141 Dose
Preparation, Sentence 3 . To clarify the actual procedure, delete this sentence and replace with the following: All dose preparations will be refrigerated until administration.
w - t 3
S I * i C . - * : = -
- 3 i * 6 0 6 2 4 >-447i
f n 60!'2< ' "227
; ' I - : t 'J- t 3 <
* . 70 39 56 * i Z= i . v ; s
147
00191$.
Amendment No. 2 Page 2
HWI 6 3 2 9 - 1 0 0
2. Page 6. 6. Experimental Design, C. Dosino Procedures. (51 Dose Analyses. (c) Routine Analyses. To clarify the actual procedure, delete this section and replace with the following:
All dose preparations will be assayed each week for the first 4 weeks. Each week thereafter, the control diet and one test material dose concentration (selected sequentially) will be collected. Samples from each 4-week period will be analyzed as a group.
3. Page 9. 6. Experimental Design. 6. Postmortem Procedures. (31 Special Analyses. To clarify the actual procedure, add the underlined as follows:
(3) Special Analyses (scheduled sacrifice animals only!
4. Page 9. 6. Experimental Design. G. Postmortem Procedures. (4) Tissue Preservation. Sentence 2 . To clarify the protocol, add the underlined as follows:
Samples of these tissues (scheduled sacrifice animals only) will be preserved in glutaraldehyde for further processing if electron microscopic evaluation is desired.
Effective December 31. 1990
5. Pace 6. 6. Experimental Design. C. Dosing Procedures. (51 Dose Analyses. To include the collection of retention samples and a second homogeneity for Diet 2, delete this section and replace with the following:
(5) Retention Samples
Samples will be taken from the base diet (premix) and from each dose preparation and stored in a freezer for at least 6 months, unless used for analysis. At the discretion of the Study Director, these samples will be returned to the Sponsor or discarded.
(6) Dose Analyses
By HWI. Additional samples may be
assayed at the discretion of the Study Director.
(a) Homogeneity
One sample each from all dose levels mixed for Week 1 will be taken from the top, bottom, and two opposing sides of the dose preparation and assayed for test material content. Due to a change in the mixing procedure for the 1 ppm level, one sample from the 1 ppm level mixed for Week 3 will also be taken from the top, bottom, and two opposing sides of the dose preparation and assayed for test material content. All samples will be stored in a freezer until
analyzed.
148
00193.5
Amendment No. 2 Page 3
HWI 6329-100
(b) Stability (c) Routine Analyses
Four additional sets of samples will be taken from each dose preparation mixed for Week 1. One set will be analyzed on the day of mixing. One set will be kept for at least the same period of time and under the same conditions that are expected to be used for the study, then analyzed. The remaining two sets will be stored in a freezer and assayed after 2 and 7 weeks.
All dose preparations will be assayed each week for the first 4 weeks. Each week thereafter, the control diet and one test material dose concentration (selected sequentially) will be collected. Samples fpom each 4-week period will be analyzed as a group.
Roger4 . Perkins, PhD
Oipi ornate, ABT Study Monitor 3M
AMENDMENT APPROVAL
I/n / n
Date
R e c e i v e -
JAN 2 1991
H azleton W iscon sin
Study Director Toxicology Hazleton Wisconsin, Inc.
Dati? /
__iStLn/i________________________________ Representative Quality As'SiH/ance Unit Hazleton Wisconsin Inc.
(ADM/jle) 01/10/91
_______ L'/S> "`ff/ Date
149 091.91^
HAZLETON
W333I- iSNjCMiON NeoSjiINo
90S- O f ' . c t SO* 75* S
WIO'SON S CONSi N 53707
HWI 6329-100
i O O N N M O u w r m S t n c n C e -cir,
PROTOCOL TP9321
13-Week Dietary Toxicity Study with T-5180, Ammonium Periluorooctanoate (CAS No. 3825-26-1) in Hale Rats
HWI 6329-100
Sponsor
3M Toxicology Services Building 220-2E-02, 3M Center St. Paul, MN 55144-1000
Study Monitor
Roger G. Perkins, PhD, 0ABT
Contractor
Hazleton Wisconsin, Inc. 3301 Kinsman Boulevard Madison, WI 53704
Study Director
Matthew J. Palazzolo, PhD, DABT
Amendment No. 2 Effective December 10, 1990
This amendment modifies the following portion of the protocol:
1. Page 9. 6. Experimental Design. G. Postmortem Procedures. (4) Tissue Preservation, and Amendment No. 1. Item 4 . To clarify tissue collection
for possible electron microscopic evaluation, delete this section and replace with the following:
(4) Tissue Preservation
The following or representative samples will be collected and preserved in 10% phosphate-buffered formalin, unless otherwise specified:
Lesions Brain Liver
Lungs Testes (one testis) . Accessory sex organs (seminal
vesicle, prostate, coagulating gland, and urethra)
U ! # d S 1 I I I 1 G f ** i n y J 4 P 4 *
Prior.# 6 0 8 - 2 4 1 - 4 4 7 1
*l* 608*241-7227
. f r I f: e U r> t o K ; * $ Z Z "
T i # 7 0 3 9 5 6 h a ^ r a . - nO S
_:
GO
150
Amendment No. 2 Page 2
HW I 6329-100
Samples of the following tissues (scheduled sacrifice animals only) will be preserved in glutaraldehyde for further processing if electron microscopic evaluation is desired:
Brain Liver Lungs Testes (one testis) Accessory sex organs (seminal vesicle, prostate)
AMENDMENT APPROVAL
Study Monitor 3M
Diplomate, ABT Study Director Toxicology Hazleton Wisconsin, Inc.
Representative Quality Assurance Unit Hazleton Wisconsin Inc. (ADM/jle) 01/21/91
Date
Oate / 7
Date
Received
JAN 3 1 1991 H azleton W isco n sin
00131$
HAZLETON
WISCONSIN
* OI T O ff ic i SOI M4$ M AD ISO N W ISC O N SIN $3707 7445
HHI 6329-100
4 C O R N IN O 14MW4t<wv $ v r.^ CnTr^vir.*
PROTOCOL TP9321
13-Week Dietary Toxicity Study with T-5180, Ammonium Periluorooctanoate (CAS No. 3825-26-1) 1n Hale Rats
HWI 6329-100
Sponsor
3M Toxicology Services Building 220-2E-02, 3N Center St. Paul, MN 55144-1000
Study Monitor
Roger 6. Perkins, PhD, OABT
Contractor
Hazleton Wisconsin, Inc. 3301 Kinsman Boulevard Madison, HI 53704
Study Picsc-tor
Matthew J. Palazzolo, PhD, OABT
Amendment No. 3
This amendment modifies the following portions of the protocol:
Effective November 30. 1990
l. Pa93_2...STWYIDSHTifLCftllflN Proposed Studg-IteetiH * , Experimental
Termination Date. Because the final date of data collection has been determined, delete *To be provided by amendment* and replace with the following:
March 29, 1993
Effective April 8. 1991
2. Page 8. 6. Experimental Design. E. Special Analysis 121 Test Material and Residue Analysis. Sentence 2 . At the Sponsor's request to analyze serum for test material residue, delete this sentence and replace with the following:
An aliquot of serum (see Section 6. E. 1. Hormone Analysis) will be collected and stored until analysis for test material residue.
^P O n I
60824144*'
F r, s v ? " E S S M A 1I 0 i ,, , *
3 3 0' r n $ / 4 N l O J l t U * :
r j * 609 2 4 * 7 2 2 *
M 0 - 5 * *. *.\ 'A s* \ S .?.
` * *,'4
152
COlSl
Amendment No. 3 Page 2
Study Monitor 3M
AMENDMENT APPROVAL
HWI 6329-100
3
Toxicology Hazleton Wisconsin, Inc.
'-Pd/iA. Representative Quality Assurance Unit Hazleton Wisconsin Inc.
153 coiaig
3H Gtntral Office
JR Center St. Paul, RN 55144-1000 (412) 733-1110 Dun No.I 00-417-3062
haterial safety
DATA SHEET
facsihile copy
HWI 6329-100
DIVISION! INDUSTRIAL CHEHICAL PRODUCTS DIVISION TRADE NAHEi FC-143 FLUORAD Brand Fluorocheaical Surfactant 3R I.D. NURBERi 79-0211-0008-0 P8-02I1-0347-0 78-0211-0871-7
78-0211-1522-7 ZF-0002-0378-4
ISSUED! Harct) 28, 1787
SUPERSEDES! July 27, 1788 DOCUHENTi 10-3809-2
1. INGREDIENTS
CAS NO.
EXPOSURE U N I T S
PERCENT VALUE UNIT
TYPE AUTH
Aaooniua Ptrfluoroalkyl
Carboiylato Aaooniua Ptrfluoroalkyl
Carboaylato Aaaoniua Parfluoroalkyl
Carboiylat Aaaoniua Parfluoroalkyl
Carboiylat*
3825-24-1 4130-43-4 21415-47-4
48257-11-0
w
7S.0-100.0 0.1
< 5.0 0.1
< 5.0 0.1
< 5.0 0.1
aq/a3
aq/a3
aq/aS
aq/aS -
TMA TMA TMA TMA
SOURCE OF EXPOSURE LIAIT DATA! - ACOIHt Aaerican Conforme* of Sovernamtal Industrial Hyqlenlst - 3Ni 3R Eipoturo Guidelines
ACGIH 3R 3R 3R
2. PHYSICAL DATA
BOIL2NQ POINT................. ..
VAPOR PRESSURE............... N/A
VAPOR DENSITY!................. N/A
EVAPORATION RATCi.............. N/A
SOLUBILITY IN MATERI.... .
>10 *
SPECIFIC GRAVITY!............. 0.377-0.500
(Bulk)
PERCENT VOLATILE.............. N/A
VOLATILE 0 R 8 M U C S I ............. N/D
pH !...............
CA. 5.00
(0.5* Aqueous)
VISCOSITY!......................N/D
APPEARANCE AND ODORI Llqht colortd pouderj sllght odor.
3. FIRE AND EXPLOSION HAZARD DATA
FLASH POINTi.................. Non-Flaaaable FLARRABILITY LIAIT -LELi..... N/A
FLARRABILITY L1R1T -UELl..... N/A AUT0I6N1TI0N TERRERATURE ...... N/D
EXTINGUISHING HEDIA! Matar, Foao, C02. Dry Cheaical
fikk-o
tMAR W/A
Aa foi
->8d W
JfR >
it f
154
C019^
MSDSi F C - H 3 FLUORAD Brand Fluorochoaical Surfactant Mar-28-lW
3. FIRE AND EXPLOSION HAZARD DATA
(continued)
3M
PAGE 2
--------------- H W I g 3 2 9 . 1 0 0
SPECIAL FIRE FIGHTING PROCEDURESt Non* Known
UNUSUAL FIRE AND EXPLOSION HAZARDS! H*at of fir* aay r*l*as* a u o n i a and HF.
d. REACTIVITY DATA
STABILITY! Stable INCOHPATABILITY - MATERIALS TO AVOID!
NA
HAZARDOUS POLYHERIZATIONI Mill Mat Occur HAZARDOUS DECOMPOSITION PRODUCTS!
Th*raal decoapotition aay product toxic aattrial* including HF.
3. ENVIRONMENTAL INFORMATION
SPILL RESPONSE! Obttrv* prvcaution* froa oth*r **ction*. Usa toiic dust aatk. Ut* a*t sweeping coapound, or wat*r to avoid dusting. Collect spi1ltd
aattrial. Placo in a clottd containor.
RECOMMENDED DISPOSAL! Rii with flaaaablt aattrial and incintratt in an industrial or coaaoreial facility. Coabustion products ili includ* HF. Discharg* spont solutions to a aasttaattr tr*ata*nt systoo. Roduc* discharg* rat* if foaaing occurs. Sinct rtgulations vary, consult applicablt r*gulations or authoritits btfor* disposal. U.S. EPA Hazardous Mast* No.I Non*
ENVIRONMENTAL DATA! Chtaical Oiyg*n D*aand (COD)i Nil (700 ag/Kg)| 20-Day lioehtaical Oxygon Doaand (B0D20)i Nil| fd-Hr. LCSO, lluogill Sunfish (Lopoait aacrochirus)! Sdt ag/L| fd-Hr. LCSO, Fathtad Minnoa(Pia*phal*S proa*las)I 76* ag/L| dG-Hr ECSO, Daphnia aagnaz 032 ag/L| Id-Day ECSO (coll dry waight), 6rttn Algat (S*l*nastrua capricornutua) 73 ag/L.
SARA HAZARD CLASS! FIRE HAZARD! No PRESSURE! No REACTIVITY! No ACUTEi Yts CHRONICi Yt*
0. SUGGESTED FIRST AID
EYE CONTACT!
laatdiattly flush vitti pltnty of vator. Continuo for 10 oinutts. Call a physician. SKIN CONTACT! Mash afftcttd aroa vith soap and vattr. INHALATION! If syaptoas occur, r*aov* parson to frtsh air. If syoptoos continu*, call a physician. INGESTION!
DO NOT INDUCE VOMITING. Givo copious aaounts of vattr. M E D I A T E L Y call a physician or Poison Control Cantor. OTHER FIRST AIDi
Abbreviations! N/D - Not D*t*rain*d
H /t - Not Applicable
155
001920
nSDSs FC-143 FLUORAD Brand Fluorochemicai Surfactant Har-28-lt8f
PAGE 3
A. SUGGESTED FIRST AID
NOKC
7. PRECAUTIONARY INFORMATION
Use only in oil ventilated areas uith sufficient local exhaust vontilation to Maintain alrborno levels at recognised health and safety levels. Do not breathe dust. Do not set poeder or airborne dust in eyes) wear personal eye protection. Do not get on skin} eear
impervious gloves and protective clothing. Wash hands thoroughly after use before eating or drinking. Do not take internally. Launder contaeinated clothing before refuse. NOTES FC-143 uas fed to albino rats for 2 years} no compound Induced carcinogenicity earn found in the study. There uere statistically significant compound related benign testicular tumors. Based on current knouledgei the findings have no human health implications. P REVENT MOISTURE CONTAMINATION TO KEEP POWDER FREE FLOWING. SPECIAL PROTECTION
CYC PROTECTIONS Safety Goggles SKIN PROTECTIONS Rubber gloves protective clothing. VENTILATIONS Local eihaust ventilation should be used to control airborne dust. RESPIRATORY PROTECTIONS Toxic dust or supplied air respirator if dust is not mell controlled.
ADDITIONAL EXPOSURE LIMITS
~ EXPOSURE L I M I T S ----!
INGREDIENTS
VALUE UNIT TYPE AUTM
3M
K y i 5329-100
Ammonium Perfluoroalkyl Carboxylate 0.1 og/m3 TWA 3M
SOURCE OF EXPOSURE LIMIT DATAs - ACGIHs American Conference of Governmental Industrial Hygienists - 3Ms 3M Exposure Guidelines
8. HEALTH HAZARD DATA
EYE CONTACTS FC-143 can be irritating to the eyes upon direct contact. Animal studle* Indicate FC-143 Is moderately irritating to the eyes. Airborne FC-143 may cause eye injury consisting of corneal opacity. SKIN CONTACTS FC-143 may be absorbed through the skim. Slightly toxic uhen absorbed through the skin. FC-143 is non-irritating to the skin. INHALATIONS FC-143 may cause respiratory system irritation from inhalation of dust. FC-143 can be considered moderately toxic by inhalation on a single exposure} a median lethal concentration for a 4-hour exposure in the albino rat is ftO milligrams per cubic meter. Repeated inhalation exposure produced liver changes and elevated blood organofluoride levels in rats. INGESTIONS FC-143 is considered moderately toxic from a single oral exposure. The acute oral LDSO (rat) is 340 milligrams per kilogram of body uelght. FC-143 is not teratogenic (rats or rabbits). See
Abbreviationss N/D - Not Determined
N/A - Net Applicable
156
00192
HSDSi F C - H 3 FLUORAD 8rand Fluorocheoical Surfactant Har-28-lt8t
8. HEALTH HAZARD SATA
(continued)
precaution for result of 2 year study.
RAGE 4
OIY5
HWI 6329-100
Abbreviation! N/9 - Not Determined
N/A - Not Applicable
The information on this Data Sheet represents our current data and best
opinion as to the proper use and handling of this oaterial under noraal
conditions. Any use of the oaterial uhieh is not in eonforaance with this
Data Sheet or hich involves using the aetertal in coebinatlon with any
a* ' `or * x d b f i j * r sr j n v
* t h i b r % QQr\ * jb 1 1 1 tV O f t h # y $ # r .
157 CO102p^
HWI 6329-100
APPENDIX B Individual Antemortem Observations
Individual Body Weights (g) Individual Food Consumption (g/wk) NOTE: Only observations other than normal are indicated in the Individual Antemortem Observations tables.
158
G019
AN IHAL NUMBER
67451
67452 67452 67452
67453
67454 67454
67455
67456 67456
67457
67458
67459 67459
67460
67461 67461
67462
67463 67463
67464 67464
67465 67465 67465
67466
67467
67468
67469
HUI 6329-100
INDIVIDUAL ANTENORTEN OBSERVATIONS CROUP 1 - 0 PPH T-S180 - HALES
UEEK
OBSERVATION
LOCATION
6 INTERIH SACRIFICE 01/25/91
1 MALOCCLUSION 3 ALOPECIA 22 RECOVERY SACRIFICE 05/17/91
21 RECOVERY SACRIFICE 05/16/91
2 ALOPECIA 14 TERMINAL SACRIFICE 03/26/91
8 INTERIM SACRIFICE 02/14/91
20 CHROMODACRYORRHEA
RIGHT EYELID
21 RECOVERY SACRIFICE 05/16/91
5 INTERIM SACRIFICE 01/24/91
8 INTERIM SACRIFICE 02/14/91
9
SMALL MOVABLE TISSUE MASS
HIND RIGHT VENTRAL
14 TERMINAL SACRIFICE 03/26/91
14 TERMINAL SACRIFICE 03/25/91
3 ALOPECIA 5 INTERIM SACRIFICE 01/23/91
14 TERMINAL SACRIFICE 03/25/91
3 ALOPECIA 8 INTERIM SACRIFICE 02/12/91
4 ALOPECIA 6 INTERIM SACRIFICE 01/25/91
3 ALOPECIA
3 BLOODY CRUST
NECK
8 INTERIM SACRIFICE 02/13/91
8 INTERIM SACRIFICE 02/13/91
6 INTERIM SACRIFICE 01/25/91
5 INTERIM SACRIFICE 01/23/91
8 INTERIM SACRIFICE 02/13/91
159
ANIMAL NUMBER
67470
67471
67472
67473
67474
67475
67476 67476 67477
67473 67478
67479 67479 67479
67480
67481
67482 67482
67483 67483
67484
67485
67486
67487
67488 67*89
67490
HUI 6329-100 INDIVIDUAL ANTEMORTEM OBSERVATIONS
CROUP 1 - 0 PPH T-5180 - MALES
UEEK
OBSERVATION
LOCATION
14 TERMINAL SACRIFICE 03/26/91 22 RECOVERY SACRIFICE 05/17/91
8 INTERIM SACRIFICE 02/13/91 14 TERMINAL SACRIFICE 03/25/91
8 INTERIM SACRIFICE 02/14/91 14 TERMINAL SACRIFICE 03/22/91
4 MALOCCLUSION 6 INTERIM SACRIFICE 01/25/91 6 INTERIM SACRIFICE 01/25/91 4 ALOPECIA 8 INTERIM SACRIFICE 02/14/91 3 ALOPECIA 4 MALOCCLUSION 22 RECOVERY SACRIFICE 05/17/91 8 INTERIM SACRIFICE 02/12/91 8 INTERIM SACRIFICE 02/12/91 6 MALOCCLUSION 14 TERMINAL SACRIFICE 03/22/91 16 ALOPECIA 21 RECOVERY SACRIFICE 05/16/91 8 INTERIM SACRIFICE 02/12/91 14 TERMINAL SACRIFICE 03/22/91 5 INTERIM SACRIFICE 01/23/91 14 TERMINAL SACRIFICE 03/25/91 14 TERMINAL SACRIFICE 03/26/91 INTERIM SACRIFICE 01/24/91 21 RECOVERY SACRIFICE 05/16/91
160
ANIMAL NUMBER
67491
67492
67493 67493 67493 67493
67494
6749S
67496 67496
67497
67498
67499 67499 67499
67500
67501
67502
67503
67504
67505
HUI 6329-100 INDIVIDUAL ANTEHORTEH OBSERVATIONS
CROUP 1 - 0 PP T-S180 - ALES
UEEK
OBSERVATION
LOCATION
5 INTERIM SACRIFICE 01/23/91
5 INTERIM SACRIFICE 01/24/91
2 SORES
NECK
3 ALOPECIA
9 BLOODY CRUST
NECK
22 RECOVERY SACRIFICE 05/17/91
8 INTERIM SACRIFICE 02/14/91
8 INTERIM SACRIFICE 02/12/91
20 UART-LIKE LESION
HEAD
21 RECOVERY SACRIFICE 05/16/91
22 RECOVERY SACRIFICE 05/17/91
8 INTERIM SACRIFICE 02/23/91
2 BLOODY CRUST
NECK
3 ALOPECIA
14 TERMINAL SACRIFICE 03/26/91
5 INTERIM SACRIFICE 01/23/91
14 TERMINAL SACRIFICE 03/25/91
6 INTERIM SACRIFICE 01/25/91
5 INTERIM SACRIFICE 01/24/91
14 TERMINAL SACRIFICE 03/22/91
14 TERMINAL SACRIFICE 03/22/91
161 C0192&
ANIMAL NUMBER
67506
67507
67508
67509
67510
67511 67S11 67511
67512
67513
67514
67515
67516
67517 67517
67518
67519
67520 67520
67521 67521 67521 67521 67521 67521
67522
67523 67523
HUI 4389-100 INDIVIDUAL ANTEMORTEM OBSERVATIONS
CROUP 2 - 1 PPM T-S180 - NALES
UEEK
OBSERVATION
LOCATION
6 INTERIN SACRIFICE 02/14/91
14 TERMINAL SACRIFICE 03/22/91
21 RECOVERY SACRIFICE 05/16/91
5 INTERIN SACRIFICE 01/24/91
5 INTERIN SACRIFICE 01/24/91
1 ALOPECIA
1 BLOOOY CRUST
NECK
5 INTERIN SACRIFICE 01/24/91
8 INTERIN SACRIFICE 02/13/91
22 RECOVERY SACRIFICE 05/17/91
5 INTERIN SACRIFICE 01/24/91
5 INTERIN SACRIFICE 01/24/91
21 RECOVERY SACRIFICE 05/16/91
20 NALOCCLUSION 22 RECOVERY SACRIFICE 05/17/91
14 TERNINAL SACRIFICE 03/26/91
14 TERMINAL SACRIFICE 03/26/91
4 NALOCCLUSION 22 RECOVERY SACRIFICE 05/17/91
1 ALOPECIA
1 BLOODY CRUST
NECK
2 SORES
BACK
2 BLOODY CRUST
BACK
3 SORES
NECK
14 TERMINAL SACRIFICE 03/22/91
8 INTERIN SACRIFICE 02/12/91
8 SOFT FECES 14 TERMINAL SACRIFICE 03/26/91
ANIMAL NUMBER
67524 67524
67525
67526
67527
67528
67529
67530 67530 67530 67530
67531
67532 67532 67532
67533
67534 67534
67535
67536
67537
67538 67538 67538 67538 67538
67539
67540
67541
HUI 6369-100
INDIVIDUAL ANTEHORTEM OBSERVATIONS CROUP 8 - 1 PPH T-S180 - HALES
WEEK
OBSERVATION
LOCATION
4- ALOPECIA 5 INTERIM SACRIFICE 01/23/91
5 INTERIM SACRIFICE 01/23/91
14 TERMINAL SACRIFICE 03/25/91
8 INTERIM SACRIFICE 02/13/91
22 RECOVERY SACRIFICE 05/17/91
5 INTERIM SACRIFICE 01/24/91
1 ALOPECIA
1 BLOODY CRUST
HEAD
3 SORES
HEAD
5 INTERIM SACRIFICE 01/23/91
8 INTERIM SACRIFICE 02/12/91
3 ALOPECIA
3 BLOODY CRUST
HEAD
8 INTERIM SACRIFICE 02/12/91
14 TERMINAL SACRIFICE 03/25/91
4 ALOPECIA 21 RECOVERY SACRIFICE 05/16/91
14 TERMINAL SACRIFICE 03/22/91
14 TERMINAL SACRIFICE 03/22/91
5 INTERIM SACRIFICE 01/24/91
5 ALOPECIA
5 BLOODY CRUST
ABDOMEN
5 BLOODY CRUST
LEFT FORELEG
7 BLOODY CRUST
RIGHT FORELEG
21 RECOVERY SACRIFICE 05/16/91
8 INTERIM SACRIFICE 02/14/91
8 INTERIM SACRIFICE 02/12/91
14 TERMINAL SACRIFICE 03/22/91
CGI92^
ANIMAL NUMBER
67542
67543 67543 67543
67544
67545
67546
67547
67548
67549 67549 67549
67550
67551 67551
67552
67553 67553
67554
67555
67556
67557
67558
67559 67559 67559
67560
HUI *329-100
INDIVIDUAL ANTEMORTE OBSERVATIONS CROUP 2 - 1 PPM T-5180 - HALES
WEEK
OBSERVATION
LOCATION
8 INTERIM SACRIFICE 02/13/91
6 MALOCCLUSION
18 MISSINO
TEETH
21 RECOVERY SACRIFICE: 05/16/91
6 INTERIM SACRIFICE 01/25/91
8 INTERIM SACRIFICE 02/14/91
8 INTERIM SACRIFICE 02/13/91
8 INTERIM SACRIFICE 02/14/91
22 RECOVERY SACRIFICE 05/17/91
2 BLOODY CRUST
BACK
3 ALOPECIA
14 TERMINAL SACRIFICE 03/25/91
6 INTERIM SACRIFICE 01/25/91
13 SOFT FECES 14 TERMINAL SACRIFICE 03/25/91
14 TERMINAL SACRIFICE 03/26/91
2 MALOCCLUSION 14 TERMINAL SACRIFICE 03/25/91
8 INTERIM SACRIFICE 02/13/91
5 INTERIM SACRIFICE 01/23/91
14 TERMINAL SACRIFICE 03/26/91
8 INTERIM SACRIFICE 02/14/91
5 INTERIM SACRIFICE 01/23/91
3 ALOPECIA
3 BLOODY CRUST
NECK
6 INTERIM SACRIFICE 01/25/91
8 INTERIM SACRIFICE 02/12/91
164 OOlSjUJ'
ANIMAL NUMBER
67561 67561 67561 67561
67562 67562
67563 67563 67563
67564 67564
67565
67566
67567 67567 67567 67567
67568
67569
67570 67570
67571
67572 67572
67573 67573
67574
67575
67576
67577 67577
HUI 4329-100 INDIVIDUAL ANTEHORTEN OBSERVATIONS
CROUP 3 - 1 0 PPM T-S160 - MALES
UEEK
OBSERVATION
LOCATION
2 ALOPECIA
2 SORES
HEAD
2 BLOODY CRUST
HEAD
22 RECOVERY SACRIFICE 05/17/91
4 ALOPECIA 6 INTERIM SACRIFICE 01/25/91
2 SORES
NECK
2 BLOODY CRU8T
NECK
14 TERMINAL SACRIFICE 03/22/91
3 MALOCCLUSION 14 TERMINAL SACRIFICE 03/25/91
6 INTERIM SACRIFICE 01/25/91
5 INTERIM SACRIFICE 01/24/91
16 MALOCCLUSION
16 EPISTAXIS
18 HISSINC
TEETH
21 RECOVERY SACRIFICE 05/16/91
5 INTERIM SACRIFICE 01/24/91
5 INTERIM SACRIFICE 01/24/91
6 MALOCCLUSION 8 INTERIM SACRIFICE 02/14/91
14 TERMINAL SACRIFICE 03/22/91
20 SWOLLEN
TAIL
22 RECOVERY SACRIFICE 05/17/91
18 MALOCCLUSION 22 RECOVERY SACRIFICE 05/17/91
6 INTERIM SACRIFICE 01/25/91
21 RECOVERY SACRIFICE 05/16/91
5 INTERIM SACRIFICE 01/23/91
3 MALOCCLUSION 5 INTERIM SACRIFICE 01/23/91
165
00193$
ANIMAL NUMBER
67578
67579
67580
67581
67582
67583 67584
67585 67586
67587
67588 67588
67589
67590 67590
67591 67591 67591 67591 67591 67591
67592
67593 67594
67595 6759S 67596
67597
HUI *329-100 INDIVIDUAL ANTEMORTEM OBSERVATIONS CROUP 3 - 10 PPM T-S180 - MALES
WEEK
OBSERVATION
LOCATION
8 INTERIN SACRIFICE 02/12/91
21 RECOVERY SACRIFICE! 05/16/91
8 INTERIN SACRIFICE 02/13/91
8 INTERIN SACRIFICE 02/13/91
8 INTERIN SACRIFICE 02/13/91
8 INTERIM SACRIFICE 02/12/91
8 INTERIN SACRIFICE 02/12/91
8 INTERIN SACRIFICE 02/14/91
8 INTERIN SACRIFICE 02/13/91
8 INTERIN SACRIFICE 02/12/91
8 NALOCCLUSION 22 RECOVERY SACRIFICE 05/17/91
21 RECOVERY SACRIFICE 05/16/91
4 ALOPECIA 5 INTERIN SACRIFICE 01/24/91
3 SORES
BACK
3 SORES
HEAO
3 BLOODY CRUST
BACK
3 BLOODY CRUST
HEAD
4 ALOPECIA
5 INTERIN SACRIFICE 01/23/91
5 INTERIN SACRIFICE 01/24/91
21 RECOVERY SACRIFICE 05/16/91
8 INTERIN SACRIFICE 02/13/91
3 ALOPECIA 5 INTERIN SACRIFICE 01/23/91
14 TERMINAL SACRIFICE 03/26/91
5 INTERIN SACRIFICE 01/24/91
166
G0193
ANIMAL NUMBER
67598 67599 67599 67600 6760t 67602 67602 67603 67604 67605 67606 67607
67608 67609 67610 676t 1 676t2
67613 67613 67614 67615
HUI 6329-100
INDIVIDUAL ANTEMORTEM OBSERVATIONS CROUP 3 - 10 PPM T-5180 - MALE8
UEEK
OBSERVATION
LOCATION
14 TERMINAL SACRIFICE 03/26/91
3 MALOCCLUSION 5 INTERIM SACRIFICE 01/23/91
14 TERMINAL SACRIFICE 03/26/91
6 INTERIM SACRIFICE 01/25/91
2 MALOCCLUSION 8 INTERIM SACRIFICE 02/14/91
14 TERMINAL SACRIFICE 03/22/91
14 TERMINAL SACRIFICE 03/22/91
14 TERMINAL SACRIFICE 03/26/91
8 INTERIM SACRIFICE 02/14/91
14 TERMINAL SACRIFICE 03/26/91
14 TERMINAL SACRIFICE 03/25/91
22 RECOVERY SACRIFICE 05/17/91
14 TERMINAL SACRIFICE 03/25/91
14 TERMINAL SACRIFICE 03/25/91
S INTERIM SACRIFICE 02/12/91
2 BLOODY CRUST
NECK
14 TERMINAL SACRIFICE 03/22/91
8 INTERIM SACRIFICE 02/14/91
14 TERMINAL SACRIFICE 03/2S/91
167
00193^
ANIMAL NUMBER
67616 67617 67618 67619 67620 67621 67621 67621 67622 67622 67622 67623 67624
67625 67626 67627 67628 67629 67630 67631 67632 67633 67634 67634 67634
67635
67636
HUI 389-100
INDIVIDUAL ANTEMORTEM OBSERVATIONS CROUP 4 - 3 0 PPH T-S1S0 - HALES
UEEK
OBSERVATION
LOCATION
14 TERMINAL SACRIFICE 03/25/91
8 INTERIM SACRIFICE 02/13/91
14 TERMINAL SACRIFICE 03/22/91
5 INTERIM SACRIFICE 01/24/91
6 INTERIM SACRIFICE 01/25/91
2 ALOPECIA
2 BLOODY CRUST
HEAD
14 TERMINAL SACRIFICE 03/26/91
9 MALOCCLUSION
9 EPISTAXIS 22 RECOVERY SACRIFICE 05/17/91
14 TERMINAL SACRIFICE 03/25/91
14 TERMINAL SACRIFICE 03/26/91
6 INTERIM SACRIFICE 01/25/91
21 RECOVERY SACRIFICE 05/16/91
5 INTERIM SACRIFICE 01/23/91
21 RECOVERY SACRIFICE 05/16/91
8 INTERIM SACRIFICE 02/14/91
6 INTERIM SACRIFICE 01/25/91
5 INTERIM SACRIFICE 01/23/91
5 INTERIM SACRIFICE 01/23/91
6 INTERIM SACRIFICE 01/2S/91
2 ALOPECIA
4 BLOODY CRUST
RIGHT FOREPAU
8 INTERIM SACRIFICE 02/12/91
14 TERMINAL SACRIFICE 03/26/91
6 INTERIM SACRIFICE 01/25/91
168
001933
ANIMAL NUMBER
67637
6763S
67639
67640
67641 67641
67642
67643 67643
67644
67645
67646
67647
67648
67649 67649 67649 67649 67649
67650
67651 67651 67651
67652
67653 67653 67653
67654
67655
HUI 4329-100
INDIVIDUAL ANTEMORTEM OBSERVATIONS CROUP 4 - 3 0 PP T-S180 - HALES
WEEK
OBSERVATION
LOCATION
21 RECOVERY SACRIFICE 05/16/91 6 INTERIM SACRIFICE 02/13/91
8 INTERIM SACRIFICE 02/13/91
5 INTERIM SACRIFICE 01/23/91
2 ALOPECIA 14 TERMINAL SACRIFICE 03/22/91
8 INTERIM SACRIFICE 02/12/91
12
SMALL MOVABLE TISSUE MASS
HIND LEFT VENTRAL
14 TERMINAL SACRIFICE 03/25/91
8 INTERIM SACRIFICE 02/12/91
14 TERMINAL SACRIFICE 03/26/91
8 INTERIM SACRIFICE 02/14/91
8 INTERIM SACRIFICE 02/14/91
5 INTERIM SACRIFICE 01/24/91
2 SORES
NECK
2 BLOODY CRUST
NECK
4 ALOPECIA
7 MALOCCLUSION
22 RECOVERY SACRIFICE 05/17/91
14 TERMINAL SACRIFICE 03/22/91
5 ALOPECIA 16 MALOCCLUSION 22 RECOVERY SACRIFICE 05/17/91
14 TERMINAL SACRIFICE 03/2S/91
12 UART-LIKE LESION
HIND LEFT VENTRAL
12 UART-LIKE LESION
LEFT HIND PAW
14 TERMINAL SACRIFICE 03/25/91
C INTERIM SACRIFICE 01/24/91
8 INTERIM SACRIFICE 02/14/91
169
ANIMAL NUMBER
67656 67656
67657 67657
67658
67659
67660
67661
67662
67663 67663 67663
67664 67664 67664
67665
67666 67666
67667 67667
67668 67668 67668
67669
67670 67670 67670
HUI 6329-100
INDIVIDUAL ANTEMORTEM OBSERVATIONS CROUP 4 - 3 0 PPM T-5180 - MALES
UEEK
OBSERVATION
LOCATION
5 SUOLLEN
LEFT HIND PAU
8 INTERIM SACRIFICE 02/14/91
4 AL'OPECIA 8 INTERIM SACRIFICE 02/12/91
14 TERMINAL SACRIFICE 03/26/91
5 INTERIM SACRIFICE 01/24/91
22 RECOVERY SACRIFICE 05/17/91
8 INTERIM SACRIFICE 02/12/91
8 INTERIM SACRIFICE 02/13/91
4 SQUINTING
RIGHT EYE
4 CHROMODACRYORRHEA
RIGHT EYE
5 INTERIM SACRIFICE 01/23/91
7 ALOPECIA
15
SMALL MOVABLE TISSUE MASS
HIND RIGHT VENTRAL
21 RECOVERY SACRIFICE 05/16/91
5 INTERIM SACRIFICE 01/23/91
2 MALOCCLUSION 22 RECOVERY SACRIFICE 05/17/91
5 ALOPECIA 8 INTERIM SACRIFICE 02/13/91
5 MALOCCLUSION 5 RH1NORRHEA >4 TERMINAL SACRIFICE 03/22/91
14 TERMINAL SACRIFICE 03/22/91
5 ALOPECIA
19
SMALL MOVABLE TISSUE MASS
HIND RIGHT VENTRAL
21 RECOVERY SACRIFICE 05/16/91
170
GOlStttf*
ANIMAL NUMBER
HUI 329-100
INDIVIDUAL ANTEMORTEM OBSERVATIONS CROUP S - 100 PPM T-51B0 - MALES
UEEK
OBSERVATION
LOCATION
67671 67671
67672 67672
67673 67673 67673 67673
67674
67675 67675
67676 67676 67676 67676 67676 67676 67676 67676 67676 67676 67676
67677
67678
67679
67680
67681 67681
67682 67682 67682
67683
3 14
11 14
2 2 4 14
21
4 5
12 13 15 15 15 16 18 20 20 20 22
14
21
14
8
2 6
1 1 5
21
MALOCCLUSION TERMINAL SACRIFICE 03/25/91
MALOCCLUSION TERMINAL SACRIFICE 03/25/91
ALOPECIA BLOODY CRUST BLOODY CRUST TERMINAL SACRIFICE 03/26/91
HEAO RIGHT FOREPAU
RECOVERY SACRIFICE 05/16/91
ALOPECIA INTERIM SACRIFICE 01/24/91
SWOLLEN
BLOODY CRUST
SWOLLEN ' STIFF
OOES NOT USE SWOLLEN MISSING
THIN HUNCHED POSTURE NECROTIC RECOVERY SACRIFICE 05/17/91
BOTH HIND PAWS TAIL RIGHT FOREPAU TAIL BOTH HIND LEGS BOTH FOREPAUS TIP OF TAIL
TAIL
TERMINAL SACRIFICE 03/26/91
RECOVERY SACRIFICE 05/16/91
TERMINAL SACRIFICE 03/22/91
INTERIM SACRIFICE 02/12/91
ALOPECIA INTERIM SACRIFICE 01/25/91
ALOPECIA BLOODY CRUST INTERIM SACRIFICE 01/23/91
NECK
RECOVERY SACRIFICE 05/16/91
G0193
ANIMAL NUMBER
7684 67684
67685 67685 67685 67685
67686
67687
67688 67688 67688 67688 67688 67688
67689 67689
67690
67691 67691
67692
67693
67694 67694 67694
67695
67696
67697
67698
67699
67700
HU1 389-100 INDIVIDUAL ANTEMORTEM OBSERVATIONS CROUP 5 - 1 0 0 PPM T-S180 - HALES
UEEK
OBSERVATION
LOCATION
9 MALOCCLUSION 21 RECOVERY SACRIFICE 05/17/91
2 ALOPECIA
2 SORES
NECK
2 BLOODY CRUST
NECK
4 MORIBUND SACRIFICE 01/16/91
14 TERMINAL SACRIFICE 03/22/91
6 INTERIM SACRIFICE 01/25/91
8 ALOPECIA 10 THIN 10 MALOCCLUSION 10 FEU OR NO FECES 11 SOFT FECES
14 TERMINAL SACRIFICE 03/26/91
3 CROOKED
TIP OF TAIL
,5 INTERIM SACRIFICE 01/23/91
5 INTERIM SACRIFICE 01/23/91
10 MALOCCLUSION 14 TERMINAL SACRIFICE 03/25/91
5 INTERIM SACRIFICE 01/24/91
5 INTERIM SACRIFICE 01/24/91
ALOPECIA
2 BLOODY CRUST
LEFT FORELEG
21 RECOVERY SACRIFICE 05/16/91
14 TERMINAL SACRIFICE 03/22/91
5 INTERIM SACRIFICE 01/24/91
14 TERMINAL SACRIFICE 03/26/91
6 INTERIM SACRIFICE 01/25/91
e INTERIM SACRIFICE 02/14/91
14 TERMINAL SACRIFICE 03/25/91
172
00193^
ANIMAL NUMBER
HUI *329-100 INDIVIDUAL ANTEMORTEM OBSERVATIONS CROUP 5 - 1 0 0 PPM T-S1B0 - HALES
UEEK
OBSERVATION
LOCATION
67701
67702
67703
67704
67705
67706 67706
67707 67707
67708 67708 67708
67709
67710
6771 1
67712
67713 67713
67714
67715
67716 67716
67717 67717
67718
67719 67719
67720
21
e
8
22
8
4
22
2 8
1 1 .8
8
8
8
14
4
6
14
5
7 14
2 5
8
3 8
14
RECOVERY SACRIFICE 05/16/91
INTERIM SACRIFICE 02/13/91
INTERIM SACRIFICE 02/14/91
RECOVERY SACRIFICE 05/17/91
INTERIM SACRIFICE 02/13/91
ALOPECIA RECOVERY SACRIFICE 05/17/91
ALOPECIA INTERIM SACRIFICE 02/12/91
ALOPECIA BLOODY CRUST INTERIM SACRIFICE 02/13/91
INTERIM SACRIFICE 02/12/91
INTERIM SACRIFICE 02/14/91
INTERIM SACRIFICE 02/14/91
TERMINAL SACRIFICE 03/26/91
ALOPECIA INTERIM SACRIFICE 01/25/91
TERMINAL SACRIFICE 03/25/91
INTERIM SACRIFICE 01/24/91
ALOPECIA TERMINAL SACRIFICE 03/22/91
ALOPECIA INTERIM SACRIFICE 01/23/91
INTERIM SACRIFICE 02/14/91
MALOCCLUSION INTERIM SACRIFICE 02/12/91
TERMINAL SACRIFICE 03/22/91
NECK
173
C0193g
ANIMAL NUHBER
67721 67722 67722 67723 67724 67725
HUI *329-100 INDIVIDUAL ANTEHORTEM OBSERVATIONS GROUP S - 100 PPH T-5180 - HALES
UEEK
OBSERVATION
LOCATION
e INTERIN SACRIP1CE 02/13/91
5 MALOCCLUSION 8 INTERIN SACRIFICE 02/12/91
22 RECOVERY SACRIFICE: 05/17/91
e INTERIN SACRIFICE 02/13/91
5 INTERIN SACRIFICE 01/23/91
174
0019 < f
ANIMAL NUMBER
67726 67727 67728 67729 67730 67731 67732 67733 67734 6773S 67736 67737 67738 67739 67740 67741 67742 67743 67744 67745 67746 67746 67747 67748
HUI 389-100 INDIVIDUAL ANTEMORTEM OBSERVATIONS
CROUP 6 - 0 PPM T-S180 - MALES
UEEK
OBSERVATION
LOCATION
5 INTERIM SACRIFICE 01/23/91
5 INTERIM SACRIFICE 01/25/91
8 INTERIM SACRIFICE 02/13/91
8 INTERIM SACRIFICE 02/14/91
14 TERMINAL SACRIFICE 03/25/91
8 INTERIM SACRIFICE 02/12/91
14 TERMINAL SACRIFICE 03/26/91
5 INTERIM SACRIFICE 01/23/91
8 INTERIM SACRIFICE 02/12/91
5 INTERIM SACRIFICE 01/24/91
14 TERMINAL SACRIFICE 03/26/91
8 INTERIM SACRIFICE 02/12/91
14 TERMINAL SACRIFICE 03/25/91
8 INTERIM SACRIFICE 02/14/91
14 TERMINAL SACRIFICE 03/22/91
5 INTERIM SACRIFICE 01/23/91
5 INTERIM SACRIFICE 01/24/91
8 INTERIM SACRIFICE 02/13/91
14 TERMINAL SACRIFICE 03/26/91
5 INTERIM SACRIFICE 01/24/91
8 SWOLLEN
TAIL
14 TERMINAL SACRIFICE 03/25/91
14 TERMINAL SACRIFICE 03/26/91
14 TERMINAL SACRIFICE 03/22/91
175
00194$
ANIMAL NUMBER
67749 67750 67751 67752 67753 67753 67754 67755 67756 67757 67758 67759 67760 67761 67762 67763 67763 67764 67765 67765 67766 67766 67767 67768 67769 67770
HUI 6329-100 INDIVIDUAL ANTEMORTEM OBSERVATIONS
CROUP 6 - 0 PPM T-S180 - HALES
UEEK
OBSERVATION
LOCATION
14 TERMINAL SACRIFICE: 03/22/91
8 INTERIM SACRIFICE 02/13/91
5 INTERIM SACRIFICE 01/25/91
8 INTERIM SACRIFICE 02/14/91
0 CHROMODACRYORRHEA
RIGHT EYE
8 INTERIM SACRIFICE 02/14/91
14 TERMINAL SACRIFICE 03/25/91
14 TERMINAL SACRIFICE 03/22/91
14 TERMINAL SACRIFICE 03/26/91
5 INTERIM SACRIFICE 01/25/91
8 INTERIM SACRIFICE 02/14/91
5 INTERIM SACRIFICE 01/24/91
14 TERMINAL SACRIFICE 03/22/91
5 INTERIM SACRIFICE 01/25/91
5 INTERIM SACRIFICE 01/23/91
2 ALOPECIA 8 INTERIM SACRIFICE 02/13/91
5 INTERIM SACRIFICE 01/25/91
2 MALOCCLUSION 8 INTERIM SACRIFICE 02/13/91
7 MALOCCLUSION e INTERIM SACRIFICE 02/12/91
14 TERMINAL SACRIFICE 03/25/91
8 INTERIM SACRIFICE 02/12/91
5 INTERIM SACRIFICE 01/25/91
5 INTERIM SACRIFICE 01/25/91
176
00194/
INDIVIDUAL 13 WK DIETARY '
Armor NUMBER
0 ___ 1
2
GROUP 1 MALES
CONTROL
67451 67452 67453 67454 67455 67456 67457 67451 67459 67460 67461 67462 67463 67464 67465 67466 67467 67466 67469 67470 67471 67472 67473 67474 67475 67476 67477 87476 67479 67460 67461 67462 67463 67464 67465 67466 67467 67466 67469 67490 67491 67492 67493
209 1 187.1 217.1 206.2 200.4 187 5 202.7 208 4 186.5 210.0 204.1 212.2 205.1 199.3 208 2 210.3 214.1 201.2 208.3 196.9 210.3 201.3 206.3 205.5 208.3 213.3 194.0 204 6 196.0 208.0 197.3 169.7 216.6 208.2 206.7 200.5 193.8 223.0 207.9 203.1 201.7 226.8 169.8
265.0 236 8 280.1 267.3 259 0 243.6 254.7 258.9 243.1 266.0 260 1 269.5 270.0 261.7 287.0 264.7 270.2 262.3 270.8 255.3 270.8 287.3 273.5 263.1 272.3 268.7 250.2 260.4 251.8 266.4 255.1 241.4 277.3 264.6 288.5 261.3 252.4 291.9 261.4 268 1 255.6 290.9 245.4
320.4 294.4 336.7 326.2 311.9 284.7 297.8 304.5 294 7 317.9 306.8 324.1 315.9 323.6 307.5 321.6 315.3 302.6 324.6 312.5 318.5 334.0 337.6 318.4 324.8 321.0 305.3 311.0 302.3 315.3 295.4 284.8 323.0 315.1 321.7 305.0 299.1 345.8 296.6 317.2 296.9 341.2 293.3
DEAD ANIMAL
3
368.1
333.3
380.8
367.7
350.3
325.1
328.4
340.3
331.3
354.8
343 3
361.2
354.2
361.9
338.6
358 9
354.1
339.8
363.3
348.8
354.3
379.0
378.5
355.5
367.0
361 2
349.6
353.0
346.7
349.1
329 8
324.5
354.9
360.0
363.3
339 331
78
392 0
329.8
357.9
331 3
385 4
323.4
4 412.3 369.1 423.0 410.3 392.4 360.8 356.4 375.7 373.9 391.1 389.9 395.9 393.9 398.1 366.0 404.7 393.2 373.8 398.9 379.8 394.0 424.1 423.2 392.4 407.2 394.2 390.7 389.8 367.9 391.5 363.5 355.3 382.8 402.9 405.9 379.6 360.0 435.5 3SS.0 394.6 365.9 424.6 349.3
5
444.5 398.4 459.5 438.9 418.3 388.1 398.1 401.2 422.05 424.9 427 1 426.7 387.9 434.4 423.07 431.5 402.0 425.3 455.9 446.5 424.0 428.8 425.5 425.4 415.7 392.5 418.3 392.3 386.0 413.7 434.5 434.*6 396.2 469.07 421.*1
* 368.9
*tffr6T00
-j
BODY WEIGHTS (G) rOXICITY STUDY WITH T-51I0
HWI 6379-100
TEKSr 6 7 4 0 10 11 12 13
* 415.0 444.1 463.4 430.3 407.7
ft
414.5 421.3 437 f.t0 447.0 447.ft5 402.0
459.3 ft ft
453.3 422.5 443.2 441.5 474.1 442.2 4 4 0 .ft0
ft
430.1 407.5 434.3 404.4 300.5 431.4 452.4 4 5 7 .ft0
413.0 4 0 4 .ft0
4 3 4 .ft0 ft
344.3
ft
435 8 508 1 479.7 452.4 424.ft4
420.8 436.5 455.ft6 473.5 464.ft7 414.3
4 7 5 .ft6 ft
476.7 437.1 464.3 504.3 492.2 454.2 4 7 1 .ft5
ft
454.6 430.6 453 0 423.7 411.4 452.4 473.6 4 7 9 .ft4
424 4 5 2 0 .ft4
4 5 4 .ft6 ft
407.6
ft
452 5 527 1 496.ft5 455.7
ft ft
454 2 469.ft2 404.II2
ft ft
ft ft ft ft
455 1 4 9 2 .ft1
5 1 7 .ft1
4 0 7 .ft7 ft ft
43 0.fftt6
442 4 4 7 2 .ft2
5 0 6 .ft4
450.3 535.0
ft
4 4 0 .ft0
ft
425.2
ft
477 1 544.4 514.ft6 465.5
ft ft
463.4 445.ft3 520.ft7
ft
fftt fftt
ft 472.7 517.3
ft
5 4 2 .ft9
5 1 0 .ft1 ft ft
44 9.ft1
ft
454 6 4 9 5 .ft6
52 9 ft4
467 5 555.7
ft
40 4 .fftt1
437.5
ft
440 5 562 5 531.ft4 475 ft6
ft
466 8 501.ft6 540.ft1
ft
fftt
ft
ft
ft
445.6 5 2 6 .ft2
5 5 4 .ft0
523.7 ft ft ft
4 5 5 .ft7
ft
473.5 510.0
ft
546.0 ft
461 7 5 6 7 .ft0
5 0 6 .fftt9
446.6
ft
494.5 577.7 540.ft4 444.ft6
ft
461.6 507.ft9 555.ft8
ft ft
ft ft ft ft
400.7 5 3 0 .ft0
5 6 0 .ft4
5 4 1 .ft2 ft ft
4 4 6 .ft6
ft
490.2 5 2 0 .ft6
5 6 5 .ft3
406.4 5 4 0 .ft4
5 1 3 .ft4
ft
463 4
. ft
494.9 592.4 553.ft2 406.ft5
ft
440.6 522 ft4 569.ft7
ft
ft ft ft ft ft .
496.4 5 4 7 .ft3
5 4 9 .ft6
5 4 9 .ft3 ft ft
44 3 .ft3
ft
497.9 5 3 3 .ft6
5 7 7 .ft2
505 4 597.7
ft
5 2 2 ft6
ft
472.7
ft 507.4 600.4 565 ft3 510.II6
ft 496 5 S 2 6f.t6 566 ft1
ft
ft ft ft ft ft
514 4 5 6 5 ft4
5 9 4 ft2
5 6 6 ft4 ft ft
471 1 ft ft
513 4 5 3 9 .ft7
5 4 9 .ft0
514 2 6 1 0 .ft5
5 3 6 fftt8
444 9
''GTGO
INDIVIDUAL BODY WEIQHTS (0) 13 UK DIETARY TOXICITY STUDY WITH T-5110
ANIMAL NUMBER
14 15 18
OROUP 1 MALES
CONTROL
7451 674S2 7453 7454 7455 7458 7457 7451 87458 7480 7481 87482 07483 87484 87485 87468 87487 87461 87469 67470 67471 7472 67473 87474 67475 67476 67477 6747 67479 67410 67481 67482 67483 67484 67485 67486 67487 67488 67489 67490 67491 67492 7493
A
512.2 619.3
a
0
517.19
0
* *
0
<8
0
0 0
*
0 0
0
578.3 0
0
1
0
* 490.6
0
0
556.4 0
0
0
0
* * 545.7
A
484.3
0
527.2 623.5
0
525.1 0 0 0 0 0 0 * 0 0 0 0 0 * 0
588.4 0 A A A A A A
509.0 A
A A
564.8 A
A A A
A
A
553 5 A A
497.2
A
533.8 631.8
A A
540.4 A A A A A A A A A A A A A A
605 5 A A A A A A A
514.2 A A A
580.1 A A A A A A
566.5 A A
494.4
* DEAD ANIMAL
17
A
540.6 636.8
A A
541.5 A A A A A A A A A A A A A A
615 1 A A A A A A A
526.6 A A A
589.4 A A A A A A
583 5 A A
505.7
1
A
541.4 641.2
A A
543.8 A A A A A A A A A A A A A A
616 1 A A A A A A A
528.1 A A A
585.7 A A A A A A
574.3 A A
507.9
"WEEKS-- 19 20
A
554.7 649.5
A A
557.2 A A A A A A A A A A A A A A
628:1 A A A A A A A
524.6 A A A
597.5 A A A A A A
587.7 A A
509.9
A
S55.0 659.7
A A
561.7 A A A A A A A A A A A A A A
838 1 A A A A A A A
517.5 A A A
601.1 A A A A A A
596 0 A A
508.3
21
A
579.0 A A A A A A A A A A A A A
A A A A
648.0 A A A A A A A
524.7 A A A A A A A A A A A A A
5110
oo
HUI 6329-100
INDIVIDUAL BODY WEIGHTS (Ol 13 WK DIETARY TOXICITY STUDY WITH T-51IO
wmwt NUMBER
OROUP 67494 67405 67496 67497 67491 67499 67500 67501 67502 67503 67504 87505
---------------------------------------------wrorc 012345 6
1 MALES
CONTROL
206.5 203 6 204.5 187.1 208 5 212 3 216.0 202.6 188.6 197.2 205.9 204.2
258 9 271.6 256.3 242 0 268.3 274 5 269.3 256.0 250 3 259.5 267.2 257.1
305.4 328.2 299.6 294 5 321.6 331 6 317.1 302 5 302.9 309.8 324.6 304.3
333.0 370.4 330.8 341 0 366.9 377 0 3S4.4 338.1 344 4 352.4 362.9 342.6
367.2 406.1 361.6 373.9 410.0 417.3 384.7 373.7 379 4 387.5 403.4 375.2
389.7 433.9 386 8 403.1 438.1 452.4
A
401.2 412.5
A
437.8 402.8
414 4 454 9 404 1 421 1 461.3 479.0
A
415.4 A
A
467.0 427.8
7
430.6 474 8 421.6 440.0 488.9 502.6
A
436.7 A A
492.5 44S.2
8
A A
435 4 459.2
A
512.8 A
455 1 A A
509.5 463.3
9
A A
445 2 478.2
A
536.5 A
475.5 A A
522.6 478.7
10
A
A 452 7 483 5
A
551 8 A
473.9 A A
559.5 494.8
11
A A
463.8 501.2
A
560 4 A
492.4 A A
573.0 513 2
HWI 6379-100
12 13
A
A
469.6 507.2
A
569.1 A
493.1 A
A
579 4 527.7
A A
479 8 518.7
A
576.6 A
510 2 A A
596 4 543 9
O O
C3 A
*45:
DEAD ANIMAL u>
INDIVIDUAL BOOY WEIGHTS (Ql 13 WK DIETARY TOXICITY STUOV WITH T-S1I0
ANTHAL NUMBER
GROUP 67494 67495 67496 $7497 6749$ 67499 67500 67501 67502 67503 67504 67505
14 15 ___ 16__
1 MALES - CONTROL
*
A
4SI .9 521.*3
A
A A A A A
A
A A
4*0 8 533.5
A A A A A A A A
A A
4SI 1 549.9
A A A A A A A A
_V
A A
491.S 559.7
A A A A A A A A
IS
A A
492.S 565.3
A A A A A A A A
TJETR5-- 19 20
A A
49S.5 576.2
A A A A A A A A
A A
499.2 590.2
A A A A A A A A
21
A A
592.5 A A A
A A A A
HUI 6379-100
oO DEAD ANIMAL CD
INDIVIDUA! 13 WK DIETARY
ANIMAL NUMBER
01
GROUP 2 MALES
6750# 67507 67506 67509 67510 67511 67512 67513 67514 67515 67516 67517 67511 67519 67520 67521 67522 67523 67524 67525 67526 67527 67528 67529 67530 67531 67532 67533 67534 67535 67536 67537 67531 67539 67540 67541 67542 67543 67544 67545 67546 67547 67548
202.0 204.4 199.8 223.6 201.3 221.4 200 3 207.9 220 2 192.0 208.3 212.5 201.8 201.2 216.4 202.4 199.3 223.1 185.2 214.8 196.8 195.9 197.6 201.8 223.5 213.1 213.2 201.2 218 0 197.8 208 6 180.7 192.3 201.5 217.1 207.1 194.3 200.0 198.6 200.9 212 6 210.6 190.8
256.0 2 S 9 .1 259 4 287.3 256.4 271.5 248.9 264.3 282.1 240.4 260.5 275.3 254.0 254.3 270.9 259.1 263.8 286 3 235.3 277.2 254.3 257.5 255.2 250.5 279.8 277.5 271.2 261.0 280.1 252 0 264.6 232 6 258.2 258.3 283.2 278.1 245.4 261 2 254.3 259.5 268.7 271 9 243.8
DEAD ANIMAL
23
1.0000 PPM
299.4 309.3 308.2 342 4 303.0 316.2 292.0 305.6 334.1 287.8 311.8 324.4 303.7 301.1 317.2 307.2 318.8 342 2 283.5 330.4 302.3 312 7 298.8 300.5 330.0 329.9 320 2 310.8 330.5 293 3 315.1 275 7 313.8 317.8 343.3 323.7 285.0 317.1 298 2 301 4 311.3 313.7 288.1
331 8 343.6 346 5 390 3 342.0 343.6 325.7 342 5 370.8 328 1 357.7 363.6 333.4 335.4 355.2 339.0 373.0 380.0 321.3 370 3 334.8 353.9 338.7 341.7 372.2 374.2 361.2 350 8 374.8 341.9 346.2 308.0 357.9 381.2 393.6 363.3 314.6 357.9 334 2 333 7 347 9 358 9 321.7
4 363.6 388.9 388.2 432.8 377.5 374.0 361.0 370.8 402.1 365.9 392.6 399.6 365.0 372.7 383.0 373.1 412.3 419.7 357.0 409.9 366.9 389.7 366.0 380.9 401.1 418.4 399.2 385.7 408.6 375.9 385.2 337.9 397.1 398.6 439.3 393.8 351.1 397.7 361.2 360.2 384.8 383.1 353.2
5
394.8 409.5
406.ft9
88 390.0
400.8f8t7
428.4 428.7 382.5 397.2 406.6 402.2 452.0
447.fftt9
389.4 427 9
395.ft5 ft
450.2 431.1 418.8 439.5 403.5
419.ft0
422.9 431.8 477.1 421.7 377.2 432.2 389.4 380.7 418.2 405.5 386.3
C0194
00
. BODY WEIGHTS (Gl TOXICITY STUDY WITH T-S180
HWI 6329-100
T3EEK5----------------------------------------------------6_______ 7_______ 8_______ 9______ 10______ U ______ 12______ 13
413.5 429.5 421.2
*
A
411.0 425.*4
A
451.1 445.7 394.7 414.2 421.7 423. 414.7 481.*5
* 404.7 453.1 417.*0
* 478.5 451.5 447.3 482.5 424.2 442.A8 449.5 459 5 504.7 442.7 397.9 440.0
A
400.0 442.5 432 1 399.4
421.0 453.1 441.4
A A
A
432.5 449.1
A
A
483.1 488.8 407 5 432.0 438.8 4.48.7 513.0 495.9
A A
415.9 477. 437.1
A
A
503.7 473.7 48 8 482.3 449.1 482.4
A
471.4 486 9 532.4 458 2 417.0 457.A7 421.2 458.0 439.0 417.4
A
46 3 461.3
A
A A
A
474 4
A A
505.7 491.6 415.1 454.1 45.7 473.9
A
513 2 A A
433.9 A
449.2 A
A
A
A
465 . 504.9 469.3 469.3
A
491.5 A
A
482 7 A
479.9 A
A
A A
433 4
A
411.5 486.9
A A
A A
483.7 A
A
529.6 506.4 425.7 467.7 474.0 492.8
A
540 2
A A
453.5 A
467.0 A A
A
A
503 3 516.3 493.0 505.2
A
507.8 A
A
499.9 A
500.1 A
A
A
A
452.7
A
494.0 491.8
A A A A
513.1 A A
543.2 516.6 432.2 474.0 485.5 514.9
A
553.9 A A
465.9 A
477.8 A A A A
524 0 531.7 498.4 518.5
A
527.1 A A
522.5 A
511.2 A A A A
465 0
A
504 8 502.5
A
A
A
A
525.*6 *
564.3 535.1 448.7 487.8 496.5 524.*2 575.*8
* 483 *4 489.m3
* *
A
539 3 546 9 516.8 538.0
A
548.8 A
A
533 0 A
514.2 A
A
A
A
471 8
A
510.5 505.7
A A A A
539.8 A A
581 8 546.1 459.6 497.0 497.3 540.8
A
566.1 A A'
496.0 A
499.8 A A A A
558 6 550 1 530 1 549.3
A
565 2 A A
545 2 A
510 6 A A
A A
475 2
A
536.7 517 6
A A A A
557.8 A A
599.5 560 4 468.7 508 2 5111 559.3
A
606.3 A A
510 0 A
512.5 A A A A
572 8 565 5 548 5 567.7
A
582.5 A A
561 0 A
517.4 A A A A
489 8
001947
INDIVIDUAL BODY WEIGHTS (Gl 13 WK DIETARY TOXICITY STUDY WITH T-S1S0
ANIMAI NUMBER
____U ____ IS
GROUP
87506 67507 67501 67509 67510 67511 67512 67513 67514 67515 87516 67517 67511
67519 87520 67521 67522 67523 67524 67525 67526 67S27 8752 67529 67530 67531 67532 67533 67534 67535 67536 67537 67531 67539 67540 67541 67542 67543 67544 67545 67546 67547 67541
2 MALES
*
A
522 9 A
A
* *
570 6
a
601. 5 6 9 .ft1
ft 495 fAt0
ft ft
ft ft ft
516.3
* * *
573.ft6
ft
ft 604.*4
* * 512 2
ft
ft
ft
ft
492.5
ft ft
5 2 1 .ft0 ft ft ft
5 1 4 .1
617.9 57 7 .ft3
ft
524.A *
ft
i * ft
515.*9
*
513 fftt9
ft 614.
ft 517 2
*
A
491.7
* DEAD ANIMAL
16 17
1.0000 PPM
ft ft
5 3 4 .*4
6 0 0 .2 *
625.6
5 9 3 .fftt1
530.9
ft
ft
529.*3
*
A 592.ft6 ft ft 640.A ft * 516.0
A
A
A
SOI. 4
ft ft
5 3 1 .ft2 ft ft ft
6 2 1 .i0 *
631.
6 1 1 .ft7 530.ft9
*
*
ft * ft
533.1
* *
601.ft3
ft ft 641.*2 * * A 514 6
ft
ft
ft
ft
523.3
It
ft ft
5 3 7 .ft3 ft ft ft
6 2 2 .i6 *
645.6 612.6
ft ft
531.1
ft * ft * * ft *
537.ft5
ft * *
604.*5
ft
649.A1 ft ft
ft
521.8 ft
ft
ft
ft
516.0
'WEEK5-- 19 20
ft ft
5 4 9 .3 A ft
6 3 7 .ft9 ft
656.4
629.*2
*
534.2
i A * A
*ft 545.A3
A A * A 606.A6 A A 662.ft7 ft ft ft 535.0
A
A
A
*
537.
ft ft
5 5 0 .ft2 ft
6 5 6 .A5 ft
663.4
609.*6
ft
545.
ft A ft
*
556.ft3
* * * 611.*6 A A 677.A0 A A 511.3
ft
ft
ft
ft
546.7
21
ft
A
ft ft ft ft ft 673.
a a a
617.4 a a
543.
ft ft ft ft
fftt
564.*2
a a
*
a a a a a
a
a a
a
a
a
*
550.0
CMD
HVI 6329-100
ANIMAL NUMBER
GROUP 67549 67550 67551 67552 67553 67554 675S5 67556 67557 67551 87559 67560
0 _______ 1 _____
2
3
2 MALES
1.0000 PPM
214.7 191.2 209.0 221.9 203.4 211.7 207.4 200.3 211 5 221.5 225.0 206.7
272.0 241.5 276.1 292.2 257.3 276.1 261.9 251.7 293.3 279.3 290.1 261.9
323.4 214.1 337.5 347.2 299.7 322.6 316.5 307.7 331.2 331.7 349.2 310.4
361.1 327.9 390.6 392.1 343.4 364 1 356 1 343.1 374.3 373.2 403.7 345.6
INDIVIDUAL BODY WEIGHTS (Ql 13 WK DIETARY TOXICITY STUDY WITH T-5190
'TTETKS-- 45 6 7 99
405.6 359.9 423.1 419 1 391.9 402.6 399.7 376.4 413.2 409.3 447.6 399.0
443.5 391.1 453.5 452.7 411.7 432.5
A
407.3 445.2
A
492.6 426.9
465.0 A
479.9 475.1 436.4 454.6
A
429.7 462.6
A
A
439.9
491.9 A
502.9 502.1 455.2 477.9
A
445.9 492.9
A
A
459.2
520.7 A
521 9 529 4 469.9
A A
465.7 A A A A
541 0 A
534 5 553.1 497.6
A
A
493.6 A
A A
A
10
554.7 A
540.3 566 7 517.2
A A
494.9 A A A A
11
589.0 A
552.9 592.9 527.6
A A
507.7 A A A A
HUI 6329-100
12 13
591.9 A
564.7 595.2 540.9
A A
517.2 A A A A
601.5 A
574 9 599.9 549.7
A A
527 9 A A A A
$fr6T09
* DEAD ANIMAL 0to0
INDIVIDUAL BODV WEIGHTS (O) 13 WK DIETARY TOXICITY STUDY WITH T-5110
ANTHAT HUMBER
GROUP 87549 67550 87551 87552 87553 67554 67555 67556 67557 67551 67559 67560
------------------------------------------------- WTTK5-------14______ 15______ 16______ 17______ ______ li______ 20______ 21
2 MALES -
1.0000 PPM*
HUI 6329-100
* DEAD ANIMAL
oo
INDIVIDUAI 13 WK DIETARY
ANIMAI NUMBER
0 1 ____ 2___ 3
GROUP 3 HALES
10.0000 PPM
675*1 7562 7563 7564 67565 7566 7567 7566 6756 67570 67571 7572 67573 67574 67575 7576 67577 7576 757 7560 7511 752 7563 7564 67565 7566 67567 67566 6756 675S0 67591 7592 67593 7594 7595 7596 7597 67596 67599 67600 67601 67602 67603
204.6 211.3 214.7 221.2 195.9 203.2 213.0 205.3 164.6 216.7 161.1 212.6 205.3 199.3 193.9 200.4 207.3 206.4 169.0 213.4 210.1 206.6 196.6 209.4 166.2 162.4 21 6 166.3 194.2 197.4 195 1 190.1 197.7 211.6 199.2 165.0 166.5 166 3 209.6 212.4 210.7 220.7 216.0
259.7 272.4 262.2 263.5 250.4 263.0 271.2 256.5 242.7 293.2 232. 270.3 256.4 251.0 249.5 251.1 260.2 266.6 245.4 262.5 266.9 262.9 247.6 266.3 241.5 230.6 276.6 242.9 255.0 249.6 246.6 235.7 255.1 277.5 254.3 234.3 242.0 239.1 265.7 276.5 276.0 266.2 263.0
306.1 320.4 256.6 333. 295.4 234.2 323.1 226.2 292.3 340.0 262.4 315.4 304.5 299.4 308. S 299.0 296.9 312.2 292.3 305.3 316.3 311.0 288.9 321.2 285.6 273.5 324.0 289.5 311.5 300.0 294.2 270.8 314.0 334.8 308.4 279.2 262.6 288.6 312.4 330.6 337.0 337.7 254.7
345.2 366.1 378.6 376.5 332.6 354.6 366 6 343.7 320.7 362.5 320.6 351.4 333 6 332.0 359.2 334 319.0 346.2 333.3 341.1 355.3 353.2 330.2 367.0 324.8 306.7 366.3 326 9 355.5 331.0 333.7 299.2 365.5 379.3 355.8 317.0 319.2 325 0 344.9 364 1 384.6 368.3 389.0
DEAD ANIMAL
4 374.6 401.4 417.4 413.2 366.5 393.1 402.9 377. 359.7 421.5 3S0.2 396.9 374.4 365.7 404.2 360.5 347.9 380.2 389.3 370.6 391.2 388.3 369.7 408.5 360.7 336.0 405.7 364.6 388.7 364.0 359.6 321.0 409.3 417.9 390.3 346.3 356.4 360.2 361.5 398.8 434.6 409.1 430.8
S
401.9 433.2 453.1 443.2 390.0 435.0
A
448.8 370.1 406.1 400.8 394.8 440.4
*
411.5 402.7 393.7 420.3 408.9 396.8 438.8 397.3 361.7 432.6 400.2 429.1
A A
444.6 451.0 375.3
A
390.5 429.6 477.0 439.6 471.9
001950
eOnD
. BODY WEIGHTS |G1 TOXICITY STUDY WITH T-5110
HWI 6J29-100
unitey 7 6 9 10 11 12 13
4 44
4
72664461.f4..*Af.t1t1SB
4434 5329 1314 f....t7213
474.ft5 ft
436.5 425.7 416.1 446.7 433.6 416.0 464.1 410.1 362.6 447.3 415.6
457.fAAt4
474.6 477.4
393.ft1 414.ft1 454.3
466.5 492.4
4 44
4 89
2 79
.fA..t190
4 6 6 fff.ttt0
4444 7421 1493 f....t5460
406`.ft2 ft
456.1 442.9 427.4 464.1 446.1 437.9 465.9 425.9 396.6 474.0 439.1
473.fAftt9
496.2 500.6
413.ft6 436.ft6 460.0
465.3 514.0
4 55
60 2046
ff.tt394
5 1 4 fffftttt7
444 364 177 f...t757
527.ft6 ft ft
465.ft6
ft ft ft ft ft ft ft
447.3
499.A3 ft
ft
519.ft4
429 ft9 4SS ft9 495.3
ft 543.4
479 A7 555225 .ft73 5 3 7 ffff.tttt4' 444 475 459 .f..t599
546 ft2
fftt 479.ft1
ft ft ft ft ft ft ft 455 4
510.fftt4
ft
553.A3
444 ft1 476.ft1 516 6
ft 566.5
4 55
94 46 16
f.ftt150
5 5 4 ffff.tttt4
444586646 ft511
564.Aft3
ft
494.ft7 ft ft ft ft ft ft ft
478.6
523.fftt8
ft
558 ft4
456 ft2 484 ft5 536 6
ft 570 7
5 0 5 A7 55 95 04 .. 36
ft
5 7 0 ffff.tttt4 444 797051 .f.t466
581.ft7 ft ft
506 ft6 ft ft ft ft ft ft ft
491.8
536.Afftt3
581 ft3
474 ft2 499.ft7 553.3
ft 513 8
5 65
20 0654
..fftt176
5 1 1 f.fAfttt1
44497163
.3 f..t63
599.A1
ft ft
516.ft0
ft ft
fAt
ft ft 506 5
552 ft3
ft ft 596 ft9
414.ft2 516.ft4 576 9
ft 603.6
5 56
3 61
9 95
f..f.tt754
5 9 2 f.ffftttt1
554180590 f.t062
602 ft4 ft ft
530 ft4 ft ft ft ft ft ft ft
515 2
562 fAt6
ft
621 ft5
500 ft1 531 ft0 582 9
ft 609 5
^S6T00
INDIVIDUAL BOOY WEIGHTS (01 13 WK DIETARY TOXICITY STUDY WITH T-5110
ANTHX NUMBER
-------------------------------------------------- WEEKS 14 15 16 17 18 19 20
GROUP 3 MALES
10.0000 PPM
67561 67562 67563 67564 67565 67566 67567 67566 07569 67570 67571 87572 67573 67574 67575 67576 67577 67576 67579 67560 67561 67582 67583 67584 67585 67586 67587 67588 67569 67590 67591 67592 67593 67594 67595 67596 1 67597 67596 67599 67600 67601 67602 67603
547.5 A A A A
A
616 5 A A A A
525.2 5 0 S .6
A
626 3 A A A
536 $ A A A A
A
A A
521.2 563.2
A
A A
630 7
562.7 A A A A A
631.4 A A A A
527 6 513.4
A
647.1 A A A
542 5 A A A A A A
A A
528.9 561.1
A A A
648.0
574.5 A A A A A
614.1 A A A A
531.9 521.1
A
650.2 A A A
558.6 A A A A A A A A
536.3 582.6
A A A
684.3
580.0 A A A A A
622.1 A A A A
537 5 529.2
A
673.5 A A A
570.6 A A A A A A A A
546 2 592 8
A A A
676.5
587.8 A A A A A
612.9 A A A A
525.5 490.3
A
674.8 A A A
568.6 A A A A A A A A
551.4 597.6
A A A
686.6
597.2 A A A A A
636.4 A A A A
532.4 500.6
A
687.8 A A A
574.3 A * A A A A A A
563.3 603.0
A A A
694.3
605.7 A A A A A
637.4 A A A A
539.9 516.0
A
704.6 A A
A
583.0 A A A A A A A A
569.0 612.4
A A A
704.0
DEAD ANIMAL
21
613.9 A A A A A A A A A A
544.0 525.2
A A
A A A A A A A A A A A
581.5 A A A A A
0cn0
HVI 6329-100
XRimr NUMBER
GROUP 7804 7805 67808 67607 67601 67609 67610 67611 67612 67613 67614 67615
01
3 MALES
221 4 201.3 201.1 197.3 194.7 215.3 212 0 193.5 190.9 210.1 205.6 216.0
273.1 264.9 263.2 251.6 252.3 216.2 261.1 246.6 246.5 269.5 270.2 273.3
23
10.0000 PPM
328.0 310.4 316.5 219.0 294.2 331.2 313.2 297.2 291.0 323.7 323.6 317.0
362.2 352.7 350.1 324.7 327.3 314.9 356.2 3 2 S .4 336.4 364.7 362.2 355.9
INDIVIDUAL 800V WEIGHTS (G) 13 WK DIETARY TOXICITY STUDY WITH T-5110
"WEEKS-- 45 6 7I9
396.6 369.3 315.7 355.2 360.1 424.7 313.7 360.0 375.2 403.4 395.9 370.9
421.1 415.5 417.9 311.4 315.9 4SI. 1 411.4 315.5 415.6 431.1 429.0 403.3
440.7 433.9 437.0 397.7 403.3 413.1 429.1 402.7 434.7 480.1 455.6 417.2
451.9 453.1 451.3 425 2 421.5 514 8 445.3 429.2 456 6 414.1 474.5 437.1
480.3 414.1
A
444 9 434 5 537.2 471.5 442.*6 502.*0 449.0
500.2 503.*6 462.3 450.0 558 5 411.6 464.9
A
523.*5 469.5
10
511.1 516.*3 475.5 464.7 582 1 501.4 471.3
A
530.3 A
411.1
11
526.5 530.1
A
413.3 479.4 599.8 512.2 492.2
A
551.1 A
500.4
HUI 6329-100
12 13
540.7 550.4
A
491.5 411.1 617.6 529.7 505.5
A
561.2 A
512.1
555515..13
A
511 2 497.2 619 4 542.1 520 7
A
579 1 A
524 1
o DEAD ANIMAL o
CP
u \ 0v9l
INDIVIDUAL OOOY WEIGHTS (G) 13 WK DIETARY TOXICITY STUDY WITH T-SitO
mmz
NUMBER
GROUP
67604 67605 67606 67607 67608 67609 67610 $7611 67612 67613 67614 67615
---------------------------------------- weeks-------
14______ 15______ 16______ 17______ 1$______ 1______ 0______ 21
3 MALES
* 10.0000 PPM
*
632 1
* *
* *
635 4 *
* *
* * *
659 0
669.1
* *
*
612.6 *
**
* * **
696.6 *
*
*
711.2 *
a
728.0
AAAAAAA
*** ** ** * ***
Ittfl 6J?9 100
s 6T00
* DEAD ANIMAL
c0o0
INDIVIDUAL 13 WK DIETARY
ANIMAI NUMBER
01
GROUP 4 MALES
7616 67617 6761 67619 67620 67621 67622 67623 67624 67625 67626 67627 67621 67629 67630 67631 67632 67633 67634 67635 67636 67637 67631 67639 67640 67641 67642 67643 67644 67645 67646 67647 67641 67649 67650 67651 67652 67653 67654 67655 67656 67657 67651
209 2 114.1 211 5 212.1 222.7 207.7 220.9 193.7 193.3 196.2 207.7 19 0 220.1 219.9 217.2 193.2 207.5 203.4 204.2 166.9 197.1 189.6 193 1 211.7 117.1 113 5 206.5 119.4 215 3 210 6 206 2 115 9 209.1 164.2 205.4 215.7 204 9 201 8 191.7 212.2 195.0 215 1 199.3
261 0 236.0 274 8 269.5 291.8 265.9 271.1 245.3 249 2 251 6 264 5 252 6 290 2 211.1 276 2 246.7 261.1 266.0 266.0 249 1 255 0 231 3 254 S 271.4 247.9 236.1 277 1 237.6 275 4 279.0 264.7 238.6 265.1 236.3 267.6 277.2 259.3 257.6 254.4 267 7 254 5 270.8 255.8
DEAD ANIMAL
23
30.0000 PPM
228.9 277 6 328.6 248.7 335.8 313.4 323.6 293.5 296.3 288.2 253.6 300.0 322.2 337.0 331.3 254 5 301.6 290.9 309.1 296.2 293 1 282.0 303.2 326.7 296.3 279.7 333 4 280.2 320.2 331.8 313 4 281.1 313 8 285.1 322.2 327.7 295.7 318.8 292.1 313 7 305 7 322.4 295.0
357 8 310 5 370.4 351 7 390.3 354 5 366 6 313.7 332.8 327.7 334.6 334 2 386 3 379.1 375.9 324.6 335 5 375 3 349 1 336.2 326.0 314.7 344.9 366.8 335.3 315.2 378.7 309.8 351 0 380 4 353 3 317 4 346.2 321 0 364 3 348.5 327.9 362 2 298.0 351 8 341 2 354.5 328.6
4 393. 339.4 403.6 385.4 429.7 393.1 400 2 340.0 361.6 355.8 366.3 363.9 427.7 407.8 418.2 356.8 364.7 415 4 379.0 367.9 353.3 343.7 375.9 395.0 365.0 364.9 441.0 363.3 407 0 447.3 381 4 346.2 373.0 357 1 397.9 377 2 347.8 401.5 320.3 384 4 376.8 392.7 348.8
5
427.3 362.2 442.3
A
450.9 426.5 427.0 366 1 390.2 377.6 386.8
A
451.6 438.8 451.7
A A
451.2 401.2 404.8 382-0 363.3 404.1 421.3
A
371.1 433.5 355.4 409.1 463 0 414.0 374.6
A
391.3 422.5 403.6 373.4 439.1
A
413.4 388.3 422.7 369.8
CD
BODY WEIGHTS (G) TOXICITY STUDY WITH T - 5 U 0
HWI 6379 100
"WEEKS 6 7 8 9 1 0 1 1 12 13
450.9 319.1 466.4
A a
451.0 443.4 315.4 414.4
405.7
470.1 454.1
A A A A
425. 427.3
361.9 430 6 439.6
390.5 455.3 371.0 421.5 493.9 432.0 394.2
415.2 446.7 414.5 394.8 467.9
A
436.7 414 0 440.5 373.4
473.8 404.6 488.2
A A
480.4 454.5 399.4 434.0
425.9
499.1 471.4
A A A A
454 5 451.5
396.8 466.4 461.0
418.8 478.5 387 9 442.6 516.2 457.6 418.7
426 8 467 2 449 5 418 7 489.8
A
459 0 443.3 456.4 391.1
494.4 A
505 4 A A
508 2 478.4 414 3 452.6
442.2
514.7 A A A A A A
479.3
414.0 A A
444.5 A
404.7 A
540.6 A A
446.9 489.3 474 3 440 4 508 3
A A A A
410 4
515.4 A
529.2 A A
524 1 481.2 425 4 468 .0
447 .7
529.8 A A A A A A
495.8
428.9 A A
465.1 A
416.9 A
564 0 A A
466.9 502 9 489 0 453 8 525.7
A A A A
417 9
525.7 A
538 9 A A
540.8 488 5 432.7 475.5
459.9
540.6 A A A A A A
500.0
432.4 A A
479.4 A
422.0 A
565.2 A A
487 7 510 6 500 4 465.6 543.5
A A A A
430.7
549.2 A
561.7 A A
560.7 497.7 446.6 495.9
472.9
563.1 A A A A A A
513 0
446.6 A
493.9 A
434.9 A
589.9 A A
504 1 525 1 515.4 477.4 558.1
A A A A
436.8
555.8 A
574.5 A A
577.2 505.7 455.4 510.1
479.3
571.2 A A A A A A
522 3
452.1 A A
506 8 A
441 .6 A
597.5 A A
519 8 533 0 527 3 488 8 570 3
A A A A
442 9
570.1 A
591.2 A A
596.6 512 1 464 5 518.0
499.9
575 3 A A A A A A
538.5
459 0 A 4
529.1 A
452 7 A
614.6 A A
532.4 550 8 543 9 499 8 577 1
A A A A
451 4
INDIVIDUAL BOOV WEIGHTS (431 13 WK DIETARY TOXICITY STUDY WITH t-5110
6r>
ANIMAL NUMBER
14 15
GROUP 67616 67617 67616 67619 67620 67621 67622 67623 67624 67625 67626 67627 67624 67629 67630 67631 67632 67833 67634 67635 67636 67637 67636 67839 67640 67641 67642 67643 67644 67645 67646 67647 67646 67649 67650 67651 67652 67653 67654 67655 67656 67657 67656
4 MALES
*
A A
A
* 518 3
a
*
A
466.6 A
594.4 A A A A A A A A
474.9 A A A A A A
A A A A A
539.4 A
544.3 A A A A A A A
A A A A A A
517.9 A A A
502.5 A
6012 A A A A A A A A
462.6 A A A A A A A A A A A
543.3 A
558.2 A A A A A A A
* DEAD ANIMAL
16 17 30.0000 PPM
A A A
A A A
526.3 A A A
516.4
A
614.6 A A A A A A A A
494.6 A A
A A A A A A A A A
547.4 A
570.5 A A A A A A
A
A A A A A A
537.6 A A A
520.4 A
630.9 A A A A A A A A
508.4 A A A A A A A A A A A
559.0 A
579.6 A A A A A A A
16
A A A A A A
536.5 A A A
522.9 A
636 8 A A A A A A A A
511.5 A A A A A A A A A A A
556.4 A
582.8 A A A A A A A
"WEEKS-- 19 20
A A A A A A
547.2 A A A
539.5 A
646.4 A A A A A A A *
523.4 A A A A A A A
A A A A
573.4 A
595.9 A A A A A A A
A A A A A A
553.0 A A A
547.1 A
659.9 A A A A A A A A
519.1 A A A A A A A A A A A
560.4 A
602.6 A A A A A A A
21
A A A A A A
559.6 A A A A A A A A
A A A A
A A A A A A A A A A A
592.3 A
811.3 A A A A A
A
voo
HUI 6329-100
INDIVIDUAL BOOV WEIGHTS (OI 13 WK DIETARY TOXICITY STUDY WITH T-5110
ANIMAI NUMBER
----------------------------------------------------- WFFK5 012345 6
OROUP 4 MALES
3 0 . 0 0 0 0 PPM
87859 67860 67661 67662 67663 67664 6766S 67666 67667 67661 67669 67670
205 9 201 6 1113
20 6 199 1 214 194 6 219 7 201 6 210 7 209.5 203.2
270 1 265.0 245.3 263.3 255.7 269.5 249.1 276.6 259.6
277 6 266.9 263.7
314 1 311 4 214.6 30*.2 306.2 3 0 1 .* 299.0 319.1 311.2 331.5 315.2 320.3
355.6 349.0 317.0 337.4 346.5 354.7 335.7 357.0 360 5 370.5 355.3 362.2
390.9 379.9 344.1 364 7
311.0 392 1
367.5 319.5 396.2 401.1 3(1.3 391.
A
409 0 363.1 386.4
A
427.2 A
413.2 426.6 416.9 407.4 416.7
A 424.7 315.6 397.1
A
446.0 A
430.1 449.9 443.1 423.9 443.4
* DEAD ANIMAL
7
A
444. 405.1 413.1
A
473.9 A
450.3 475.0 462.3 439.6 462.3
1
A
466. A A A
504.7 A
471 6 A
472.2 462.3 479.7
9
A 471.0
A A A
524.3
A
419.1 A
416.1 470.5 417.4
10
A 4 1 9 A. 1
A A
543.4 A
496.1 A
500.1 415.5 505.4
11
A 491.7
A
A A 556.1
A
510.6 A
514.6 491 5 520.9
HUI 6329-100
12 13
A
499.3 A A A
577.4 A
511.0 A
515.0 509 6 533.1
A 514.0
A A A
590.9 A
531.3 A
50 . 4 517 4 544 4
O 6
CA
INDIVIDUAL BOOY WEIGHTS (Gl 13 WK DIETARY TOXICITY STUDY WITH T-51IO
wnwt NUMBER
-------------------------------------------- wrrre 14 15 16 17 16 19 20
GROUP 4 MALES
30.0000 PPM
67859 67660 67661 67662 67663 67664 67665 67666 67667 67666 67669 67670
A
516.5 A ft ft
607.*1 549.1
A
*
A
550.1
A
519.4 A A A
621.1 A
546 3 A A
A
560.3
A
530.6 A A A
634.6 A
563.4 A A A
571.1
A
526.6 A A A
643.3 A
572.5 A A
A
562.4
A
539.6 A A
A
650.0 A
575.8 A ft A
591.4
A
545.3 A A
A
661.ft2
584.7 A A
A
606.3
A
548.0 A A A
876.7 A
590.8 A A A
611.2
* DEAD ANIMAL
21
A
562.0
fAt
A
ft
A
605.6 A A A A
HUI 6379-100
S6T00
tNo
Oo
h*
CD
en
Animal Ntuber
__
O 1234
C67671 C67672 C67673 C67674
C67675 C67676 C67677 C67678 C67679 C67680 C67681
C67682 C67683 C67684 C67685 C67686 C67687 C67688 C67689 C67690 C67691 C67692 C67693 C67694 C67695 C67696 C67697
C67698 C67699 C67700 C67701
C67702 C67703 C67704 C67705
C67706 C67707
214.8 219.0 193.4
225.8 198.8 211.5 200.7 189.1 187.2 192.3 195.2 199.2 197.4 206.5 199.0 199.7 206.3 186.4 183.6 212.9 193.6 212.4 208.5 191.5 185.6
216.3 214.8
199.3 188.8 210.4 195.9
208.8 212.8
207.8 191.5 201.8 206.6
246.8 265.6 244.2
279.9 258.5 271.5 254.1 238.7 230.0
242.8 241.4 248.7 250.4 251.4 240.3 258.8 257.9
232.5 243.2
257.3 234.5 264.0 256.1 236.8 226.1 264.4 259.3 245.1 239.1
256.0 238.1 266.3 262.9 255.6 249.5 252.9 258.8
291.9 307.7 287.6 316.1 309.6 318.6 298.6
281.0 273.1 283.7 288.7 290.8 299.5 292.2 274.5
308.2 304.4
272.3 290.3 297.6 261.1 310.2 307.0 273.0 254.5 294.7 293.5
286.3 280.2
296.3 285.8 314.3 296.3 294.9 305.8 296.7 314.0
313.3 348.2 321.0 356.0 349.2 353.2 337.7 320.7 297.3 308.4 310.4 322.4 340.1 326.1 302.1
351.8 332.0 304.5 331.3 328.0 272.9 340.7 346.2
310.3 280.2
314.6 320.8 322.5 321.9 329.1 321.2 353.2 335.6 331.2 350.7 321.3 354.9
349.9 375.6 355.2 385.5 382.9 385.6 371.3 352.8 318.5 341.5 337.8 420.6 369.1 355.4
*
384.7 356.1 322.0 362.0 344.1 291.4 376.9 377.1 337.6 300.9 334.6 342.3 357.0 358.9 361.9 245.6 379.7 372.6 354.5 387.2 345.3 387.6
* Dead animal
Indivicfcjal Body Weight Data (g)
Week 5 6 7 8 9 10
100 Don T-5180
388.2 407.3 382.3 422.0
*
414.3 403.9 38S.2 336.6 380.8 369.6
*
398.9 380.0
*
420.0 382.2 335.7
313.1
*
367.1 331.6
*
366.0 390.7 395.9 404.0 373.3 411.2 405.2 385.8 422.4 373.9 423.8
410.5 431.8 406.4 442.9
437.3 424.8 408.6 348.8 404.3
420.0 395.6
445.2
352.9 * *
325.8 * *
391.1 344.7
*
389.8 *
421.7 428.0 397.9 433.5 431.3 408.3 447.1 400.2 457.5
429.0 457.1 434.0 467.9
*
457.4 447.5 431.3 372.1 421.7
441.8 413.5
*
462.9
372.5 * *
348.3 *
412.5 360.3
*
403.3 *
438.6 452.3 410.7 454.2 460.0 424.5 475.4 417.1 485.8
453.5 471.8 439.4 492.0
*
472.0 465.9 442.1 384.1
* * *
463.4 422.2
*
475.9 *
383.5 * *
359.4 *
435.9 359.2
*
412.3 * *
475.2 425.7
*
446.6 *
430.4 *
474.7 491.5 452.7 513.2
469.8 486.1 457.9 395.1
# *
483.1 432.8
*
499.4
397.1
373.8 * *
445.8 362.7
*
429.5 * *
486.0 438.8
*
464.2
448.8 *
491.0 499.0 462.9 520.1
442.3 497.2 471.8 406.4
* *
497.2 432.2
*
509.9 *
339.1 * *
358.8
462.8 369.4
441.1 * *
508.7 454.1
* *
478.9 *
463.9
11
507.8 466.8 471.3 535.3
*
448.1 507.1 481.6 419.0
506.7 446.3
527.8 *
371.5
378.8 *
478.4 376.5
456.5
526.4 463.4 * 494.8 * 470.4 *
12
524.1 469.2 485.6 546.2
*
443.1 515.9 493.0 431.4
* * 522.1 450.6
537.9 *
376.8
389.3 *
493.5 376.7
* 467.9
536.1 473.3
507.0
482.4
13
543.1 464.4 496.0 558.1
*
411.3 530.1 501.6 438.6
* 530.6 462.0 * 550.2 *
400.0
402.5 * *
512.8 386.0
* 481.7
554.2 484.0
513.7 *
496.8
HUI 6329-100
Individual Body Weight Data (g)
An Inai Number
C6767I C67677 C67673 C67674 C6767S C67676 C67677 C67678 C67679 C67680 C6768I C6768? C67683 C67384 C67685 C67686 C67687 C67688 C67689 C67690 C6769I C67697 C67693 C67694 C67B95 C67696 C67697 CG7698 C67699 C67700 C6770I C67707 C67703 C67704 f67705 C67706 C677G7
570* 9
416ft 4
506 3 ft ft ft ft
538 7 463 1
ft
ft
ft ft
ft
ft
ft
ft
ft
570 !
ft
*
* ft ft ft
489 7 ft ft
535ft 9
508 3
-li.
ft ft
ft
583.3
ft
477ft 4 514ft.9
ft ft
548 6 477.4
ft
ft
533 1
ft
ft 507 1
ft
546 6 5|7 0
ft ft
ft
600.1
ft
476 9 ft
573.3 ft ft ft ft
560 3 486.5
ft
ft ft
ft ft ft ft ft
596 5
ft
510 7
558 9
57? 3
Week 17 _ U L
J3_
_2fi_
JLL
ft ft
ft
613 4
a
433ft 0 53? 5
ft
a
a
567 0 498 3
a a a * a a a *
663 8
ft
518 1
56? 9
637 8
100 onm 1-5180 (Continued!
ft ft
ft
619.7
ft
436 9 ft
533 6
a
* *
576 1 503.0
ft a a a a * *
565 9
ft ft
a
a
577 ?
67? ?
64? ?
ft ft
ft
636.6
ft
441 .8 ft
540ft.8 ft ft ft
578.9 508.5
ft ft
ft
ft
ft
ft
ft
a
578 1
a
*
* * *
538ft 5 ft
58? 1 ft
550 9 ft
ft ft
ft
644.5 ft
369.8 ft
544 8 ft ft ft ft
587.5 509.8
ft
ft
ft
ft ft ft ft ft ft
587.9
ft ft
ft
ft
ft 543 3
ft
591 ? ft
553 1 ft
ft ft
ft ft
ft 397ft 3
ft
a a a
503ft ? ft
ft
ft ft ft a * a a
a
594 6
56 ) 3
* Dead an ima 1
HUI 6379 100
Animal Huntoer
0 1234
C62206 C62209 C62210 C62211 C62212 C62213 C62214
C62215 C62216 C62212 C62218 C62219 C62220 C62221 C62222 C62223 C62224 C62225
210.3 189.5 202.2 208.5 218.0 223.2 193.3 212.3 209.2 195.6 220.2 220.6 228.9 215.2 201.0 223.9 208.6 199.2
263.9 236.2 255.4
256.3 266.5 223.9 225.3 260.9 259.6 245.5 221.5 221.9 262.2 258.2 246.5 269.3 262.8 241.6
311.3 264.2 303.9 302.0 314.2 312.8 242.0 301.3 298.1 289.8 316.8 312.6 329.9 298.5 292.9 316.3 298.8 228.2
352.5 290.1 340.2 332.4 354.4 335.1 264.8 326.4 323.2
316.8 354.5 351.6 325.2 334.2 330.2
346.6 335.0 309.1
362.3 308.3 369. 2 465.6 366.0
351.8 280.3 359.2 348.2 343.2 362.1 36t.8 413.8
363.2 359.6
325.1 364.1 332.4
* Dead animai
tion
Indivickjal Body Uight Data (g)
Week 56 7B
100 ccn 1-5160 (Continued)
*23.7 333.7 397.1 392.9 *20.6 375.9 298.9
371.6 *
*17.6 *13.0 *60.1 391.5 380.1 (02.8 399.5
*5*.* 350.2 *18.2 *12.8 **6.0
315.6
391.3 *
**3.0 436.7 *91.3 *15.9 *03.7 21.7 *27.3
*
*83.* 365.3 436.1 433.4 *71.9
*
3*4.6
*03.3 *
461.5 *62.0 523.8 434.9 421.3 **1.7 *52.2
*
* *
*93.9 *
354.3
*18.3 * *
5*5.3 * *
456.3 -*
*
9
* * *
511.8 *
369.5
*31.1 * *
575.0
*65.9 * *
10
* * 527.4 382.6 * *39.4 * * 600.5 * * *79.9 * *
11
* * * 539.8 396.5 * 4*8.6 * * 619.9 * *91.8
12
* * * 559.6 *03.9 * *57.3 * 641.7 * * *97.2 *
13
576.6 *17.3
*
<69.7
660.3 508.7
HUI 6329-100
Individual Body Weight Data (g)
An ima 1
fflbgr
C67708 C67709 C677IO C677I1 C67712 Cb7 713 C677I4 C677I4 C677I5 C677I6 C67717 C677I8 C67M9 C67720 C67771 C67777 C677?3 C67774 C67775
__________________________________Week__________________________________
JLJLJLJLJLJLJLJL
100 ppm T-Siao (Continued)*
ft * *
-
SI?. 4 *
* * * * * A
520.9 ft
ft ft ft ft ft ft ft ft ft
$?6 0
* *
540 0
ft ft ft ft ft ft ft ft ft ft ft a * ft
543.7
ft ft
ft ft ft ft ft ft ft ft ft
ft ft ft ft ft
547.7
ft
ft ft ft ft ft ft ft ft ft ft ft ft ft ft ft
* 557ft 1
ft
ft ft ft ft ft ft ft ft ft ft ft ft ft ft ft
558
ft ft
* Dead animal
HUI 6379 100
96T00
OOl
Animal
Humber
JL JL
_1_ 4
C61126 C61121
C61128 C61129 C61130 C6113I C61132 C61133 C61134 C61135 C61136 C61131 C61138 C61I39
C61140 C6I141
C61142 C61143 C61144 C61145 C61146 C6114I
C61148 C6149
C61150 C6151
C61152 C61153 C61154 C61155 C61156 C6151 C61158 C61159 C61160 C61161
202.6 202.2 204.1 226.1 212.8 222.3 226.3 229.1 216.1 211.5 211.2 216.5 220.6 202.3 211.2 218.1 195.6 213.0 205.2 211.5 209.2 215.8
225.6 211.4 216.4 210.9 224.8 223.9
220.1 213.8 195.1 223.2 211.3 218.3 190.4 189.1
249.0 249.9 244.5 251.2 251.8 266.6 261.9 259.1 259.1 252.1 255.6 256.1 261.1 249.2
243.4 261.1 241.3 258.6 252.1 258.9 251.5 263.6 262.1 251.1 261.1 254.2 264.1
264.6 263.3 249.6 242.1 264.4
260.6 258.1
238.6 240 4
283.9 294.8 285.4 290.6 291.1 306.9 291.2 298.6 301.3 294.2 298.5 293.3 303.2 295.0 216.6 298.1 282.1 299.9 291.8 301.4 295.8 303.4
303.9 294.1 298 6 296.8
305.6 302.0
306.1 288.2 286.1 305.1 303.0 299.1 280.9 218.6
323.2 338.4 323.3 326.2 331.3 343.2 333.1 331.1 338.1 326.8 339.5 330.3 345.8 331.8 308.6 339.3 322.5 331.9 331.0 341.5 339.1 342.1 343.5 329.9 334.5 335.8 348.3 343.8 344.2
329.1 330.0 354.1 342.5 344.5 311.0 319.5
354.3 366.4
348.9 365.1 358.1 314.3 365.0 364.8 362.8 356.0 311.9 361.6 380.2 368.4 331.3 311.4 354.6 362.1 363.1 315.8 369.1 310.4 315.4 360.2
365.1 363.2 380.9 313.3 311.0 360.6 360.6 318.9 311.9 314.3 348.1 353.0
Dead animal
-voi
*tf96T0
HUI 6329-100
Individual Body Weight Data (g)
*
378.2 399.8 382.7 403.7 400.8
*
386.3 *
404.9 408.9 408.9 401.8 359.1
* *
392.3 394.1
ft
395.6 408.1 405.0 391.4 393.0
*
417.1 413.1 410.6 386.8 388.4
ft
404.9 ft
373.9 ft
_ LWeek
__
JL
0 com T-5180
ft ft
397.2 425.9 401.5 420.3 423.4
ft
401.7 ft
422.0 431.0 425.1 425.6 372.8
ft ft
410.9 416.8
ft
409.0 426.4 426.5 407.9 418.7
ft
447.1 437.8 434.3 406.8 408.7
ft
423.1 ft
394.3 ft
ft ft
417.4 449.0 421.9 438.6 446.8
ft
412.6 ft
440.8 451.9 440.7 449.3 389.3
ft ft
428.3 433.0
ft
422.9 452.3 442.5 426.3 436.3
ft
469.6 460.0 452.4 414.3 429.1
ft
439.4 ft
413.7 ft
ft ft ft ft
435.8 ft
464.1 ft ft ft
458.5 ft
459.8 ft
405.4 ft ft ft
450.3 ft
438.3 472.0 457.5 441.6
ft ft ft ft
473.8 427.4 440.7
ft ft ft
437.5 ft
ft ft ft ft
450.0 ft
481.7 ft ft ft
473.5 ft
477.3 ft
427.7 ft ft ft
465.1 ft
446.4 489.1 476.2 460.2
ft ft ft ft
496.0 445.4 456.0
ft ft ft
454.2 ft
_1_
ft ft
ft
ft
451ft.1
490.0 ft ft ft
476.1 ft
488.7 ft
435.4 ft ft ft
469.6 ft
448.2 493.8 479.1 465.8
ft ft ft ft
501.1 452.2 461.7
ft ft ft
463.9 ft
JLL
ft ft ft ft
466.0 ft
500.3 ft ft ft
493.9 ft
502.3 ft
451.2 ft ft ft
479.3 ft
459.1 508.5 491.1 471.9
ft ft ft ft
514.4 460.6 473.5
ft ft ft
474.0 ft
_LL
* ft ft ft
477.7 ft
514.2 ft ft ft
504.4 ft
510.7 ft
461.4 ft ft ft
489.2 ft
460.1 523.4 501.1 485.6
ft ft ft ft
526.0 471.8 484.5
ft ft ft
491.0
J3_
ft ft ft ft
491.6 ft
525.8 ft ft ft
514.7 ft
526.2 ft ft ft ft ft
500.7 ft
464.5 534.2
ft ft ft ft ft ft
532.6 ft
492.7 ft ft ft ft ft
Animi Number
0 JL
?
3
4
C6776? C67763 C67764 C6776S C67/66 C67767 C67768 C67769 C67770
715. < 777.6 704.0
773.1 198.8 707.3 708.0 774.8 196.4
757.3 769.3 743.0 771.4
744.7 749.7 750.3 758.1 746.8
797.9 313.0 786.7 307.4 786.8 784.7 790.9 301.7 786.3
340.7 358 8 376.4 339 4 376 4 375.1
333 5 346.1 370.3
367.8 391 1 359.7 367.1 357.5 357.7 360.4 377.8 347.3
* Dead animaI
e> o
CD <o
V*,0
00
HUI 6329-100
Individual Body Height Data (g)
56 78
0 Dm* T-5180 (Continued)
421.0 ft
394.2 376.8 394.9 392.5
ft
*
444.9 *
413.9 396.0 417.3 413.4
ft ft
*
467.4
429.3 3B9.9 433.8 435.0
* ft
* *
453.9 *
9
* * * * 466.4 * * *
10
* * * * 472.7 ft * *
_LL
* * * * * 488.2 ft * ft
12
* * * * ft 492.5 ft ft *
13
ft ft ft ft ft 502.2 ft ft ft
INDIVIDUAL FOOD CONSUMPTION IG/WK) 13 WK DIETARY TOXICITY STUDY WITH T-5180
HUI 63?9-100
ANIMAL NUMBER
12 3 4
GROUP 1 MALES
CONTROL
67451 67452 67453 67454 67455 67456 67457 67456 67459 67460 67461 67462 67463 67464 67465 67466 67467 67468 67469 67470 67471 67472 67473 67474 67475 67476 67477 67478 67479 67480 67481 67482 67483 67484 67485 67486 67487 67486 67489 67490 67491 67492 67493
172.1 163.4 183.7 176.0 173 9 162 3 171.2 161.3 149.8 162.0 163.8 170.3 170.0 168.8 160.3 179.5 163.4 170.4 174.4 167.9 177.0 181 6 178.3 168.7 180.9 158 1 165.2 162.1 148.3 172.6 157.8 146.5 179.0 166 2 179.4 166.2 165 5 185.7 172.8 1712 157 1 188.4 148.8
185.0 171.1 201.0 198.6 203.2 171.5 179.8 171.4 165.0 181.1 173.9 184.9 172.6 189.9 166.9 187.3 170.6 169 4 185.6 188.9
*A
210.9 197.9 185.1 191.4 175 1 182.8 174.9 166.4 190 6 166.3 155.1 187.7 176 9 196.1 172.1 173.4 197.0 168.4 186.2 163.6 193.2 158.8
198.8 176.4 210 6 218.7 216.5 184.9 179.3 180 5 173.6 188.1 187.5 191.5 175.7 190.5 172.2 186.8 177 1 176.0 192.5 191 3 185.6 216 5 209 1 195 1 200.2 179.9 193.8 184.4 184.2 200.0 175.5 165.5
AA AA
201 0 171 1 179.7 205.7 171.3 194.1 171.2 203 0 159.9
209.5 190 1 217.7 222 6 222.0 187 9 181 4 186 0 178.9 193 4 201 1 196 9 184.7 190 9 178 3 200 3 187.S 180.8 196.3 192.1
AA
214 7 216 8 198.4 213 5 188.8 201.3 193.0 182 1 206.6 179 1 173.1 183.0 201 2 215.1 184 1 179.5 214 2 178.3
AA
182.5 209.5 163.8
* DEAD ANIMAL * INVALID VALUE - FOOD WASTED OR SPILLED
5
213 8 194.6 221.2 225.5 221.4 180.3
A
187.4 186.7 199.6
A
198.6 197.6 190.8 182.3 196.4 191.7
A
204.2 194.2
AA
215.5 199.6 202.1 208.3 195.2 204 0 196.3 171 1 211.2 187 9 179.5 192.7 206.4 224 1
A
189.1 217.6
A
211.4 A A
163.9
WEEKS 67
A
190.9 218.2
AA
AA
191.8 A
189 1 184.8 191.1
A
199.6 204.5
A
176.5 192.3
A
A
202.2 AA
198,3 216.1 212.7 195.8 207.4
A A
193.8 169 6 202 0 181 7 170.3 189 2 203.0 222.8
A
187.9 216.2
A
198.6 A A
164.8
A
189.5 215.8
AA
AA
200.9 A
185 5 179.2 189.5
A
195 5 198.6
A
172.5 186.2
A
A
200.4 194.5 206.3 208.2 216.1 199.2 206.8
A
A
192.9 162.9 197.2 176.4 172.0 187.6 198.4 219.5
A
183.8 204.6
A
193.6 A
A
162.2
8
A
194.1 214.4
AA A
193.6 A A
169.0 179.1
A
198.9 A A A A A A A
193.0 202.0
A
210.0 A
197.2 A A A
142.9 A A
167 9 192.4
A
222.1 A
183 2 198.5
A
167.0 A A
158 7
9
A
193.6 216.9
AA A
193.3 A A
175 7 184.2
A
205.7 A A A A A A A
198.2 198.5
A
219.0 A
206.2 A A A
169.8 A A
172 0 204 1
A
221.9 A
194.1 209.9
A
189.8 A A
164.1
10
A
182 3 209.6
AA A
188.0 A A
171.2 178.3
A
201.1 A A A A A A A
190.6 195.6
A
209.9 A
206.6 A A A
165 6 A A
1710 190.9
A
222 9 A
187 1 199.0
A
186.9 A A
160 1
11
A
190 4 205.5
AA A
188.5 A A
168 9 174 4
A
194.6 A A A A A A A AA
189.0 A
202.3 A
199.8 A A A
121.3 A A
164 2 169 5
A
223 4 A
182 8 194.6
A
178.6 A A
161.8
12
A
175.3 199.7
AA A
182.1 A A
170 7 173.8
A
195.4 A A A A A A A AA
187.0 A
208 6 A
203 8 A A A
110.2 A A
169 5 186.9
A
215 0 A AA
196.8 A
179 9 A A
167.5
13
A
172.7 197.4
AA A
180.6 A A
162.4 162.4
A
190.7 A A A A A A A A A'
181.9 A
199.0 A AA A A A
184 5 A A
1615 181 .9
A
210 6 A AA
186.4 A
175 3 A A
159 6
14
A
180 2 202.8
A A
187 5 A A A A A A A A A A A A A A
179.7 A A A A A A A
161 3 A A A
180 7 A A A A A A AA A A
1516
0019
<<oo
S6T00
INDIVIDUAL FOOD CONSUMPTION (G/WKI 13 WK DICTAAY TOXICITY STUDY WITH T-51S0
mwri NUMBER
15 16 17
GROUP 87451 67452 67453 67454 67455 67456 67457 67451 67459 67460 67461 67462 67463 67464 67465 67466 67467 67461 67469 67470 67471 67472 67473 67474 67475 67476 67477 67476 67479 67480 67411 67482 67483 67484 67485 67486 67487 67488 67489 67490 67491 67492 67493
1 MALES
CONTROL
* 185.2 200.8
A
184.8 A A A A A A A A A A A A A A
188.6 A A A A A A A
187.9 A A A
173.2 A A A A A A
174 7 A A
165.6
A
182.9 192.3
A A
184.2 A A A A A A A A A A A A A A
187.5 A A A A A A A
157.2 A A A
172.0 A A A A A A
177.2 A A
155.0
A
178.0 196.0
A A
185.5 A A A A A A A A A A A A A A
1845 A A A A A A A
180.1 A A A
176.1 A A A A A A
182.6 A A
163.7
DEAD ANIMAL
18 19
A
183.2 203 2
A A
ISS 1 A A A A A A A A A A A A A A
197.4 A A A A A A A
181 5 A A A
179 8 A A A A A A
181.2 A A
227.4
A
196.2 207.0
A A
202.3 A A A A A A A A A A A A A A
189.6 A A A A A A A
139.3 A A A
177.6 A A A A A A
190.0 A A
167.1
"TCEK5 20 21
A
185.6 210.1
A A
201.0 A A A A A A A A A A A A A A
194,5 A A A A A A A
143.2 A A A
178.2 A A A A A A
185.1 A A
163.2
A
IBS. A A A A A A A A A A A A A A A A A A
203. A A A A A A A
186. A A A A A A A A A A A A A
172.
roo\>
HVI 63?9 100
ANIMAL NUMBER
GROUP 7494 7495 67496 67497 67491 67499 67500 67501 67502 67503 67504 67505
12
3
1 MALES - CONTROL
160 5 197.1 159.0 156.4 174 9 189.9 186 8 153*.*9 177 6 171.4 169.7
163.0 210 2 172 3 171.6 195.7 199.2 176 6 163ft1 191.9 191.9 185.9
166.7 93.7 180 4 178 9 194 8 201.5 177.1 171 0 198.0 192.1 199 8 195.5
4
1710.07 176.9 184.3 199 4 208.7 186.1 1800.03 195.4 203 0 200.0
INDIVIDUAL FOOD CONSUMPTION 10/WK1 13 WK DIETARY TOXICITY STUDY WITH T-5180
"WEEKS-- 5 6 7 8 9 10
1780.08
182.4 191.0 201.5 213.*5 181*.09
0 212.1 203.0
1740.08 00
184.8 201.9 212.04
176.*0 *
210.0 203.6
169.9 206 2 185.1 182 7 205 6 209.05
172.09 0
207.6 196.5
* * 179.1 180.*3 203.09
174.06 0
200.0 192.3
* 0 176 6 179 *6 208 03 175.*4 0 204 7 190.5
0 * 1810 177.*3 204.*3 167.08 0
206.8 193.4
11
0 0 185.3 177.2 194.4 168.01 * 200 4 194.0
12
0 0 176 8 170.*9 194.*7 169.04 0 197.8 198.8
HWI 6329-100
13 14
0 0 00
162.*9 185.*2 166.04
0
194 4 196.0
6 * 00 162 0 0 * * 0 0 0 0
oo
* DEAD ANIMAL ** INVALID VALUE - FOOD WASTED OR SPILLED
C> to\> <fi
ANIMAL NUMBER
QROUP
67404 67405 67406 67407 67400 67400 67500 67501 67502 67503 67504 67505
15 16 ____ il_
1 MALES
CONTROL
IS
162.7 170.4
157.0 102.3
116050.. 62
101
INDIVIDUAL FOOD CONSUMPTION IQ/WK) 13 WK DIETARY TOXICITY STUDY WITH T-510O
"WEEKS 19 20 ___ 2J_
165.3 100.0
*
*
160.0 100.5
102
HUI 6329-100
,36X00
* DEAD ANIMAL " INVALID VALUE - FOOD WASTEO OR SPILLED
ro roo
INDIVIDUAL FOOD CONSUMPTION (G/WKI 13 WK DIETARY TOXICITY STUDY WITH T-5180
HUI 6379-100
KNIHT ----------------------------------------------- WEEKS
NUMBER
123 45 6
78
9 10 11 12 13 14
OROUP 2 MALES
1.0000 PPM
6750 67507 67501 67509 67510 67511 67512 67513 87514 67515 67516 67517 67518 67519 67520 67521 67522 67523 67524 67525 67526 67527 67528 67529 67530 67531 67S32 67533 67534 67535 67536 67537 67538 67539 67540 67541 67542 67543 67544 67545 67546 67547 67548
159.2 162.6 166.6 185.7 153.8 157.7 158.4 167.0 178.6 179 2 161.0 169 4 165.5 171.0 164.9 158 0 168.7 181.3 163.6 166.5 1S8.8 157.8 156.4 145.7 177.9 172.9 167.7 177.9 167.3 161.5 174.8 14S.2 167.7 162.9 187.9 180.3 141.0 168 2 164.7 163.6 158.2 165.9 156.3
168.1 171.7 176.7 199.8 164.7 163.7 160.3 167.7 187.7 183.4 172.8 179.4 180.3 178.1 182.1 162.6 197.2 192.4 178.8 177.7 168.5 168.2 160.9 163.2 188.8 183.5 181.9 19S.7 180.0 171.8 172.2 152.9 187.3 177 8 208.0 201.2 151.3 190.9 170.9 168.7 160.6 172.1 165.9
176.6 179.7 190.9 209.0 177.5 166.4 173.4 176.7 169.4 194.5 190.9 192.0
AA
193.1 202.7 177.3 216.9 202.5 189 5 191.6 174 2 181.1 167.7 169.8 202.8 194.3 192.7 201.7 191 2 190.5 178 6 163.0
AA
195.6 223 2
AA
165.1 205.7 179.6 175.1 166.0 173.6 172.6
162.1 191.4 195.5 221 0 188.0 175.6 169.3 187.8 195.2 210.1 192 5 191.5
AA
204 6 AA
180.8 219.2 208.9 202.3 205 1 179.1 182 5 180.5 179.3 215 2 210 2 208 3 208 1 193.7 196.2 186 5 169.0 202.0 198.6 230 1 208.7 170.4 213.3 181.7 180.9 173.7 1818 182 3
AA
195.7 192.5
A A A
185.3 195.7
A A
199 2 197.4
AA AA AA
188.5 229.7 212.4
A A
187.9 193.7
AA A A
212 7 211.0 218 5 199.1 202.6 200.3
A
204.2 202.4 237.1 210.7 176.0 219.6 183.0 179.8 182.0 183.1 193.5
AA
193.7 186.7
A
A A
182.6 197.8
A A
194.4 193.5
AA
AA AA
183.7 228.1 207.6
A A
180.9 191.2 196.6
A
A
215.6 203.9 215.3 193.8 195.7 195.0
A
219.9 199.9 224.7 206.0 176.2 189.9
A
178.4 180.1 182.5 183.5
185.7 191.8 179.8
A A A
178.8 196.6
A A
194.9 193.0
AA
202.7 183.2 184.9 230.5 210.9
A A
175.3 186.8
AA A A
218.1 204.6 204.8 104.4 203.3 192.8
A AA
202.9 223.3 206.3 170.7 180.8
A
181.5 174.3 179.7 178.7
A
186.2 176.0
A A A A
184.2 A A
191.4 193.8
AA
199.3 183.9 177.3
A
207.3 A A
181.6 A
173.9 A A A A
203.6 192.6 200.6 187.9
A
196.8 A A
200.9 A
184.6 A A A A
172 9
A
181.4 187.6
A A A A
195.7 A A
194.5 194.9
AA
204.9 192.5 188 6
A
217.7 A A
179.5 A AA A A A A
204.9 192.6 202 9 199 4
A
202 3 A A
217.5 A
180.4 A A A A
182 2
A
185.9 182.0
A
A A A
197.6 A A
187.8 183.2
AA
193.4 188.1 188.6
A
204.0 A
A
178.2 A
168.3 A A A A
199 2 189 3 186.7 200.2
A
198.4 A A
209.8 A
174.1 A A A A
175 5
A
176.3 180.1
A A A A
200.3 A A
194.7 185.5
AA AA
175.5 186.5
A
211.1 A A
189.6 A
184.3 A A
A A
194 1 188.9 184 3 203.5
A
217.1 A A
201.9 A
163.3 A A A A
175 5
A
173.9 173.2
A
A A A
200.2 A A
185.1 187.6
AA AA
175.0 184.9
A
199.8 A A
189.4 A
168.4 A A A A
198.1 177.7 201.6 200.2
A
20T .1 A A
202.1 A
149 8 A
A A A
171.3
A
168.7 165.7
A A A A
195.0 A A
ISO 4 180.2
AA AA AA
180.0 A
199.8 A
168.5 A
164 1 A A A A
197.8 177.8 195 4 197.3
A
198.6 A A
197.8 A
133.6 A A A A
164.7
A A
160.9 A A A A
189.2 A A
182 2 181.5
A A
161.4 A A A A A A A
157 7 A A A A A
174 8 A A A
210.8 A A A A
147.6 A A A A
156 1
* DEAD ANIMAL q ** INVALID VALUE - FOOD WASTED OR SPILLED
P fOs)
INDIVIDUAL FOCO CONSUMPTION (O/WKI 13 WK DIETARY TOXICITY STUDY WITH 7-51*0
HVI 6329-100
ANIMAT NUMBER
15 16
GROUP 2 MALES
67506 67507 67501 67509 67510 67511 67512 67513 87514 67515 67516 67517 67511 67519 67520 67521 67522 67523 67524 67525 67526 67527 67521 67529 67530 67531 67532 67533 67534 67535 67536 67537 67531 67539 67540 67541 67542 67543 67544 67545 67546 67547 6754
* A
15. 9 A A A A
202.0 A A
195.2 119.6
A A
175. A A A A A A A
157.7 A A A A A
177.4 A
A A
207.6 A A A A
150.3 A
A A A
172.6
A A
15. 9 A A A A
193 0 A A
194.9 1(8.1
A A
151.2 A A A A
A A A
154.0 A
A A A
A
167.9 A A A
203 1 A
A A A
110.6 A A A A
174.5
* DEAD ANIMAL
17 18
1.0000 PPM
A A
161.2 A A A A
206.2 A A
190.1 1*7.1
A A
151.2 A A A A
A
158.4 A A A A A
169.1 A A A
204.9 A A A A
149.5 A A A A
1(5.2
A A
172.9 A A A A
210.9 A A
201.7 195.6
A A
169.3 A A A A A A A
167.5 A A A A A
1(3.1 A A A
213.4 A A A A
206.5 A A A A
190.4
19
A A
173 7 A A A A
203.6 A A
200.5 203.3
A A
159. A A A A A A A
166.4 A A A A A
172. A A A
216.3 A A A A
17*.6 A A A A
196.7
"WEEKS-----------------------------------------------------------20 21
A A
170.7 A A A A
212.9 A A
194.3 166.6
A A
170.2 A A A A A A
167.1 A A A A A
173.1 A A A
217.5 A A A A
160.4 A A A A
195.1
A A A A A A A
223.4 A A A
174. A A
171.4
A A A
A A
164.6
A A A A A A A A A A A A A A A A A
205.7
6T09
ro o
ahihaL NUMBER
GROUP 67549 67550 67551 67552 67553 67554 67555 67SS6 67557 67559 67559 67560
12
2 MALES
171.1 151.6 177.5 192.1 164.6 191.5
AA
159 5 177.7 171.9 197.4 173.1
179.9 162.9 190.2 200.5 169.1 195.9 197.4 171.9 194.4 191.5 203.9 193.0
34
1.0000 PPM
195.4 177.4 202 7 193 5 191.1 211.2 203.9 175.0 195.2 196.9 215.0 199.4
216.3 179.9 209.7 201*.*5 214.9 210.5 173 3 199.6 193.6 219.6 201.2
INDIVIDUAL FOOD CONSUMPTION IG/WKI 13 WK DIETARY TOXICITY STUDY WITH T-5190
"WEEKS-- 5 6 7 9 9 10
220.9 166.4 209.6 211.3 197.1 215.*6 191.6 206.4
A
225.0 207.3
AA A
AA
211*.A 1 215.5
A
175.9 201.1
A
A
199.0
229.9 A
203.0 210.2
AA
214.5 A
175.9 199.1
A
A
194.1
221 1 A
192 1 214.5 201.4
A A
171.4 A A A A
217.5 A
196.6 218.6 199.4
A A
197.3 A A A A
217.6 A
194.3 210.2 191.3
A A
175.7 A A A
A
11
216.4 A
192.3 214.5 195.5
A A
172.9 A A A A
12
221.9 A
192.2 193.6 194.2
A A
177.8 A A A A
HVI 63?9-100
13 11
213 1 A
177 9 197 4 164 7
A A
162 4 A A A
C0197f)
* DEAD ANIMAL ** INVALID VALUE - FOOD WASTED OR SPILLED
roion
INDIVIDUAL FOOO CONSUMPTION (O/WKI 13 WK DIETARY TOXICITY STUDY WITH T-51IO
XHTJWr NUMBER
GROUP 67549 67550 67551 67552 6T553 67554 67555 67556 fill 67556 67559 67560
-------------------------------------------- BETKSr 15______ 16______ 17______ U ______ 19 20 21
2 MALES -
1.0000 PPM*
HUl 6329-100
C0197
* DEAD ANIMAL NO
at
T&6T09
INDIVIDUAL FOOD CONSUMPTION (G/WK) 13 WK DIETARY TOXICITY STUDY WITH t-5110
xrmwi NUMBER
--------------------------1_______ 2_______ 3_______ 4
GROUP 3 MALES - 10.0000 PPM
67561 67562 67563 67564 67565 67566 67567 67566 67569 67570 67571 67572 67573 67574 67575 67576 67577 67578 67579 67SI0 67511 67512 67SI3 67584 67585 87586 67587 67581 67589 67590 67591 67592 6T593 67594 67595 67596 67597 67598 67599 67600 67601 67602 67603
163.3 175.8 191.6 186 9 183.7 160.3 170.3 169.5 161.7 196.5 152.2 163.7 163.9 148.8 171.6 154.1 158.1 164.4 159.4 162.8 163.9 1S4.2 160.3 166.2 160.0
AA AA
150.5 168.6 158.7 154.6 134.5 170.8 181.0 165.6 157.2 156.6 180.3 162.7 182.9 182 9 173.7 184.7
177.3 188.3 200.1 204.4 188.1 182.9 186.1 177.1 171.0 200.0 171.6 170.4 166.3 163.4 188.6 165.5 154.7 168 7 171 8 166 9 177.5 165.8 168.2 179.7 172*.*9 111 3 163.3 175.9 173.6 167.4 138.2 188.8 197 8 111.1 167.5 158.7 170.6 176.8 196 2 196 1 187.2 282.6
187.0 199.3 208,5 202.1
AA
184.1 193.6 181.6 174.9 200.4 187.5 173.7 170.4 167.1 199.6 170.3 154 1 173.4 185.2 177.1 189.9 184.1 176.6 193.0
AA
AA
189.7 170.5 192.7 177.3 177.3 157.0 214.2 202 1 199.8 174 4 174.3 179.3 180.2 196.6 213.2 184 5 122.5
195.6 207.3 213.9 217.5
AA
195 8 191.6 188.4
AA
212.5 174.0 181.1 180.7 174.5 204.9 170.3 156.8 173.7 193.2 179.7 184.3 179 4 192.8 194.7
AA
AA AA
171.2 190 8 177 0 188.7 143.7 223.0 201 5 200 7 171.2
A
184 9 189.6 191.9 223.2 201 1 214.8
* DEAD ANIMAL ** INVALID VALUE - FOOD WASTED OR SPILLED
roo
VI
5
200.4 208.1 214.4 214.0 210.7
A
206.5 A A
216.0 177.1 181.4 190.3 179.0 213.4
A
184.6 200.0 184.7
AA
179.5 195.0 210.4
AA AA
208.2 189.3 191.3
A A
222.1 209.1
178.1
193 1
206.4 232.2 204.3 219.3
"WEEKS---67
1
196.2 A
209.4 216.3
A A
200.3 A A
177.0 177.0 183.2 184.2
A
215.2
A
184.4 200.1 186.5 196.3 173.1 188.5 211.0
AA AA
203.0 180.6 203.7
A A
222.7 209.9
AA
189.7
205.0 A
200.0 208.4
191.7 A
205.6 202.4
A A
209.9 A A
197.7 176.7 180.3 177.7
A
2211
A
179.9 193.3 110.4 200.7 177.4 182.5 213.1 203.2
AA
203.3 177.7 191.2
A A
224.5 209.1
178.1
194.3
212.1 A
194.8 211.7
189.1 A
203.8 200.4
A A
202.7 A A A AA
182.2 171.6
A AA
A A
117.8 A A A A A A A A
168.2 182.4
A A
215.6 A
168 8
186.7
199.1 A A
210.6
9
192.8 A
213 9 201.4
A A
217.0 A A A
180 5 187.5 173.9
A AA
A A
196.9 A A A A A A A A
154.0 184.5
A A
212.4 A
175.6
194.6
209.4 A A
208.5
10
192 0 A
208 1 205.5
A A
211.4 A A A AA
180.1 169.3
A AA
A A
190.0 A A A A A A A A
167 4 178.8
A A
207.6 A
171.8
185.6
208.2 A A
199.1
11
188.5 A
207.0 204.4
A A
198.5 A A A AA
180.4 158.5
A
210.7
A A
183.9 A A A A
A A A
171.3 181.0
A A
217.3 A
171.7
118 2
209 2 A A
199 1
12
187.2 A
208.0 192.8
A A
194.0 A A A AA
177.1 159.4
A AA
A A
184.5 A A A A A A A A
170.3 176.6
A A
215.4 A
AA
184.8
215.6 A A
205.2
HVI 6329-100
13 14
189.0 A
213 9 149.2
A A
193.0 A A A
171 1 177.4 155.1
A
202.0
A A AA , A
A A A A A A
160.7 175.4
A A
209.7 A
AA
179.5
205.3 A A
186.7
182.3 A A A A A
189.8 A A A A
177 4 160.1
A
203 9
A A AA A A A A A A A A
159 9 166.5
A A
209.2 A
A
A
A A A A
INDIVIDUAL FOOD CONSUMPTION (G/WKI 13 WK DIETARY TOXICITY STUDY WITH f-5110
MVI 6379 100
ANIMAL NUMBER
WEEKS-----------------------------------------------------------15 16 17 18 19 20 21
GROUP 3 MALES
10.0000 PPM
7561 67562 67563 67564 6T565 67566 67567 87561 67569 7570 67571 67572 67573 67574 67575 67576 67577 67S7S 67579 67510 67511 67512 67513 67514 67515 67516 67SI7 67511 67519 67590 67591 67592 67593 67594 67595 67596 67597 67591 67599 67600 67601 67602 67603
192.5 A ft ft ft A
195.a0 ft 0 0
180.5 172.0 210.*7
* * 111.*4 *
Ik
* ft ft ft ft 161.2 163.5
ft
ft
ft 208.5
ft
ft
ft
ft
ft
ft
ft
ft
ft
ft
192.ft1 ft ft ft ft
111.ft2 ft ft ft
170.5 161.ft2 202.ft9
ft
ft
112.9 ft ft ft ft a a a
162.3 175.4
ft
ft
ft 212.1
ft
ft
ft
ft
ft
ft
ft
ft
ft
ft
193.ft9 ft ft ft ft
171.ft0 ft ft ft
174.7 167.ft6 202.ft1
ft
ft 113.ft1
ft ft ft ft ft ft ft 163.3 172.1
ft
ft
ft 207.0
ft
ft
ft
ft
ft
ft
ft
ft
a
196.ft4 ft ft ft ft
171.ft1 ft ft ft
110.9 116.ft4 214.ft9
ft
ft
ft ft
ft ft ft ft
A
ft ft ft 179.1 181.6
ft
ft ft 216.ft6
ft
ft
ft
ft
ft
ft
ft
ft
A
195.ft1 ft ft ft ft
116.ft3 ft ft ft
175.7 145.ft3 215.7
ft
ft
ft 179.ft1
ft ft ft ft ft ft ft 177.7 117.1
ft
ft ft 217.2
ft
ft
ft
ft
ft
ft
ft
ft
ft
ft
184.ft5 ft ft ft ft
114.ft2 ft ft ft
171.4 176.ft3 223.0
ft ft
ft 111.ft1
ft
ft ft ft ft ft 171.0 179.1
ft
ft ft 209.ft4
ft ft
ft
ft
ft A
ft ft
ft
192.5 ft ft ft ft ft ft ft ft ft
186.2 167.ft1
ft
ft ft ft ft
ft ft
ft
A
ft 177.ft1
ft
ft ft
ft
ft
ft ft ft A ft
0
ft ft
ft
* DEAD ANIMAL
** INVALID VALUE - FOOD WASTED OR SPILLED
O P
rOo
C0 00
ANIMAL NUMBER
GROUP 7604 67605 67606 67607 67601 67609 67610 67611 67612 67613 67614 67615
12
3 MALES
175. S 174.9 154 167 1 156.6 203 1 163 6 155.1 149.4 171.2 170.5 171.0
112*A 0 172.1 170 6 170 2 213.1 176.0 173.1 151.4 161.2 114.5 174.2
34
10.0000 PPM
118*.A 3 178.7 179 5 167.1 225 9 190.9 171.7 174.0 205.2 187.9
*81
195.6 193 8 185.6 114.8 176.5 226.2 196.4 181.3 186.0 208.1 191.6 177.9
INDIVIDUAL FOOD CONSUMPTION (Q/WKI 13 WK DIETARY TOXICITY STUDY WITH t-SlIO
p e e k s -- 5 6 7 1 9 10
198 4 201.2 192.1 193.5 173.2 223.5 200.8
81 *
192.9 212.6 193.3 176.0
187.7 193.4 182.6 111.6 170.7 225 1 190.9 174.6 116.7 210.5 194.5 174.3
189.7 190.6 183.6 191.0 173.2 218.1 195.0 177.1 189.2 206.9 192.3 179.5
117 9 188.2
189 4 166.6 203 6 192 3 175.*3 202.7
*
172.2
196.3 191.5
*
195.8 170.2 216.8 196.2 180.1
*
208.0
8k
177.1
186.6 119.5
Ik
115.8 174.0 221.5 188.3 179.2
*
209.4
8k
111.8
11
183.6 163.2
8k
1818 168.9 211 4 184.1 174.2
*
201.6
8k
181.2
12
185.4 184.7
*
182.0 183.6 208.6 112.1 176.10 202.1
*
183.9
HWI 6329-100
13 14
115.1 176.6
8k
181.5 161.6 174.9 117.4 174.*1 193.*6 179.6
A A A A A
185 6 A A A A A A
C0197i
-4fc
* > DEAD ANIMAL * INVALID VALUE - FOOD WASTED OR SPILLED
NtO)o
wrnwr NUMBER
GROUP 67604 67605 67606 67607 67606 67609 67610 67611 67612 67613 67614 67615
15______ 16______ 17______ 18 3 MALES - 10.0000 PPM
193 6 161 1 203 2 205 1
INDIVIDUAL FOOO CONSUMPTION (G/WK1 13 WK DIETARY TOXICITY STUDY WITH T-5180
------------------------ WFTK5~ 19______ 20______ 2J_
210 9 215 6 217
HVI 6329-100
* DEAD ANIMAL
INDIVIDUAL FOOD CONSUMPTION (O/WKl 13 WK DIETARY TOXICITY STUDY WITH T-51IO
HVI 6379-100
ANIMAL' NUMBER
GROUP
ereie 67617 67611 67619 67620 67621 67622 67623 67624 67625 67626 67627 67621 67629 67630 67631 67632 67633 67634 67635 67636 67637 67631 67639 67640 67641 67642 67643 67644 67645 67646 67647 67641 67649 67650 67651 67652 67653 67654 67655 67656 878S7 67659
12
4 MALES
175.4 151.1 172.1 173 4 205.3 171.7 170.7 159.7 159.9 169.9 167.2 155.2 112.2 110.1 111.8 153 1 151.4 161.0 167.5 171.9 156.5 152.6 171.0 174.1 165.7 161.5 113.0 160.5 177.2 182 6 172.6 147.9 169.9
ft ft
177.3 180.0 159 5 163.3 152 2 165.6 171.7 161.6 160.4
111.5 160.7 194.1 177.1 210.5 230.3 117.3 169.0 170ft.ft0
161.7 167.2 231.2 117.5 193.2 161.5 165.0 119.7 259.6 172.6 160.5 160.9 178.5 116.4 110.4 169.6 195.4 157.2 183.3 192.9 181.9 142.1 180.4
172.2 190.9 271 3 163.2 177.6 158.7 171.7 116.7 172 0 242.0
34
30.0000 PPM
193.3 161.6 211.3 114 1 223.6 207.6 190ft.ft4
179.3 115.3 170.4 175.1 202.3
10.1 201.4 166.3 177.6
13.2 194.8 112.7 164.7 170.7 193.9 208.8 119.9 181.6 201.4
ftft
118.3 211 3 191.9 166.0 185.8
ft ft
198.1 119 7 161.6 119.9
21.9 117.6 187.1 189.4 166.1
202.0 182ft.ft7
204 5 230.5 215.6 195ft.ft1
191.1 190.2 174.4 181.8 209.3 190.8 222 0 161.9 171.0 215.7 195.6 116 2 170.0 174.5 207 .5
ft ft
190.8 190 5 194 6 139.5 157.4 202 1 200 4 172 6 190.5 206.5 202.1 204.4 163.4 194 8 151.7 204 0 191.0 201.8 167.0
DEAD ANIMAL INVALID VALUE - FOOD WASTED OR SPILLED
fv)
5
205.1 182ft.ft5
ft 246.5 219.0 203.0 191.1 191ft.ft1
ftft ft
211.0 194.7 226.9
ft
ft 223.6 202.3 209.6 179.0 183.8 212.7 215.5
ft
222.1 246.1
ft*
229.4 251.3
ftft
174.7 ft
210.4 203.2 206.9 161.7
ft* ft
203 7 174.2 205.3 172.6
T7PFK5-67
9
205.6 111ft.ft5
ft ft 215.1 197.7 112.4 191.ft1 177.ft5 209.3 193.3
ft
ft
ft ft 200.1 203.7
A
179.6 213.9 216.0
ft
195.2 205.1 159.1 191.3 221.9
ft*
174.6 ft
242.2 206.8 194.3 171.0
ft ft
ft
202.2 184.0 201.1 155.4
207.1 184ft.ft8
ft ft 215.6 197.1 184.6 185.ft4 177.ft1 201.5 201.1
ft
ft ft ft 202.7 201.ft7
178.9 227.9
ft ft
ft
194.4 205.5 159.6 186.6 225.2 201.5 173.3
ft
ft ft
207.9 200.1 171.5 207.1
ft
191.4 194.1 196.1 160.6
203.ft0 ftft ft ft
210.7 191ft.ft7 112.ft3 172.ft2 197.ft2
ft
ft ft ft ft 197.ft4 181.2
ft
ft
ft
196.3 ft
153.7 ft
220.7 ft
- ft
ft
173 1 203.8 191.0 174.9 197.1
ft
ft
ft
ft
163.1
9
204.ft9 ftft ft ft
215 0 119.6 115.2 185.ft1 170.ft6 204.3
ft ft
ft ft ft ft 167.2
ft
180.7 ft A ft
203.2 ft
1 5 6f.t2 220.5
ft ft ft
188.6 204 4 204.1 174.5 201.8
ft ft ft
A 166.4
10
204.ft4 ftft ft ft
206.3 185ft.ft3 178.ft6 165.ft4 200.0
ft
ft ft ft ft ft 116.7
ft ft ft
ft ft ft
196.7 A
154.1 ft
190.1 ft ft ft
193.6 203.0 191.9 172.4 202.6
ft ft ft ft
165.1
11
209.ft3 200.ft1
A 209.1 187ft.ft3 115.ft4 174.ft5 200.1
ft ft
ft ft ft ft 115.4
ft
110.5 ft ft ft
196.1 ft
154.1 ft
217.3 ft ft ft
190.1 201.3 194.4 170.2 191.0
ft ft ft
ft
164 6
12
209.ft9 206.ft0
ft 209.4 112ft.ft0 184.ft9 161.ft1 191.5
ft ft
ft ft ft ft 110.2
ft
1 7 3A. 9
ft ft
195.4 ft
1 55f.t7 207.1
ft ft ft
119.6 203 2 184 4 171 2 201.7
ft ft ft
ft
158.1
13
204.ft6 ftft ft ft
197.1 176ft.ft2 173.ft2 186.A1 115.ft5
ft
ft ft ft ft 181.1
1 7 5A. 4 A
ft
117.2 ft
1 4 9A. 6 194.3
ft ft ft
110 1 1914 184.1 1713 192.0
ft ft ft
ft
152.7
14
ft ft ft ft ft ft 163.A3 ft A 159.ft9 177.A4
ft
ft ft ft ft ft ft
ft ft A ft ft ft ft ft
ft ft
ft ft ft
175 1 ft
1 7 7f.t0
A
ft ft ft
A
ft
INDIVIDUAL FOOD CONSUMPTION (Q, 13 WK OIETARY TOXICITY STUDY Wl -5110
HUI 6379-100
ANIMKE NUMBER
15 16 17 16
GROUP <7616 67617 67611 67619 67620 <7621 67622 67623 67624 67625 67626 67627 67626 67629 67630 67631 67632 67633 67634 67635 67636 67637 67636 67639 67640 67641 67642 67643 87644 67645 67646 67647 67646 67649 67650 67651 67652 67653 87654 67655 676S6 67657 67656
4 MALES
ft ft ft ft ft ft 165.ft1 ft ft 169.ft6 176.ft5 ft ft ft ft ft ft ft 177.ft4 ft ft ft ft ft ft ft
ft
ft ft 166.ft2 166.ft2 II ft ft ft ft
ft
ft ft ft ft ft ft 160.ft2 ft ft 170.ft3 165.ft4 ft ft ft ft ft ft ft ftft ft ft ft ft ft ft ft
fftt
ft ft 175.ft6 169.ft3 ft ft ft ft
A
ft
30.0000 PPM
ft ft ft ft ft ft 172.ft0 ft ft 171.ft1 166.ft0 ft ft ft ft ft ft ft ftft ft ft II ft ft ft ft ft ft
ft II ft ft ft ft 162.ft2 ft ft 179.ft1 191.ft4 ft ft ft II ft ft ft ftft ft ft ft ft ft ft ft ft ft
ft
ft 192.ft9 190.ft0
ft ft ft ft ft ft
ft
ft 177.ft9 194.ft3
ft ft
A
ft ft ft
* DEAD ANIMAL * INVALID VALUE - FOOO WASTED OR SPILLED
19
ft ft ft ft ft ft 165.ft9 ft ft 190.ft0 190.ft7 ft ft ft ft ft II ft ftft ft ft ft ft ft ft ft
ft
ft ft ft 196.ft2 191.ft5 ft ft ft ft
ft
ft
wrors-----------------------------------------------------------20 21
ft ft II ft ft ft 160.ft3 II ft 164.ft1 167.ft0 ft ft ft ft ft ft ft ft** ft ft ft ft ft ft ft ft ft
ft
ft 196.ft6 166.ft4
ft ft ft ft
ft
ft
ft ft ft ft A ft 163.ft7 II ft ft ft ft ft ft ft ft ft ft
ft ft
ft ft ft ft ft
ft
ft ft ft 206.ft4 191.ft2 ft ft ft
A ft A
ANITW NUMBER
1 2 ____ 3____ 4
GROUP 4 MALES
30.0000 PPM
#785# 67660 67661 67662 67663 67664 67865 67666 67687 67661 67669 67670
177. 166.6 151 5 172 1 165.9 173.1 152 8 110.5 165.7 112.0 189.2 187.3
110.1 175.9 151.5 171.1 175.5 173.3 167.0 1(1.2 179 8 196.7 17. ( 111.4
191.5 114.1 163.6 112.2 171.2 1(4.6 173.9 1(6.5 1(7.7 199.6 175.0 199.2
201 0 1(9.5 165. 1(7 2 193.6 192.7 179.5 192.4 195.3 205. 194.0 193.1
* > DEAD ANIMAL
INDIVIDUAL FOOD CONSUMPTION (Q/WK) 13 WK DIETARY TOXICITY STUDY WITH T - 5 H 0
-v m s-- 5 6 7 9 10
* 192.6 167.7 1(7.*0 200.2
A
19.7 196.9 211.8 205.3 200.3
A
1(6.4 162.7 1(0.4
A
192.9 A
189.7 195. 174.5 193.7 201.3
A
1(3.5 185.0 178.0
A
191.4 A
192.6 196.7 1(2.6 1(7.2 202.2
A
1(1.9 A
A
A
195. A
184.7 A
179.3 117.0 190.3
A
1(7.5 A
A A
196.5 A
196.3 A
174.1 195.2 196.7
A
179. A A
A
194.0 A
1(5.6 A
176 9 1(9.1 192.9
11
A
173 5 A A A
194.2 A
1(2.7 A
176.7 194.6 181.
12
A
172.5 A A A
192. A
115 A
153.0 186.7 190.6
HWI 6379-100
13 14
A
174.1 A
A
A
190.2 A
1(4.6 A
130.1 1(1.1 1(5.
A
167.9 A A
A
195.6 A
177.9 A
A
177.6
b i *6T00
INDIVIDUAL FOOD CONSUMPTION (O/WKI 13 WK DIETARY TOXICITY STUDY WITH T-5180
XHTOXt NUMBER
15 16 ___ 17__ 18
OROUP 87659 67660 67661 67662 87663 67664 67665 67666 67687 67668 67669 67670
4 MALES
161.*3
*
A
193.1 171 *0
A
a 186.5
A
171.0 A A A
197.9 A
189.6 A A A
191.1
30.0000 PPM
A
171.8 A
A A
191.5 A
190.5 A
A
A
168.8
A
183.6 A A A
199.9 A
194.5 A A A
199.3
DEAD ANIMAL
WEEKS 19 20 21
A
181.1 A
A
A
206.1 A
204.9 A
A A.
198.4
A
171.6 A
A
A
204.5 A
197.5 A A
A
192 1
A
179.4 A A A A A
203.4 A
A A
HWI 6329-100
----------------
Animal Wimtoer
1
45
C67671 C67672 C67673 C67674
C67675 C67676 C67677 C67678 C67679 C67680 C67681 C67682 C67683 C67684 C676B5 C67686 C67687 C67688 C67689 C67690 C67691
C67692 C67693 C67694 C6769S C67696 C67697 C67698 C67699 C67700 C67701
CA7702 C67703 C677M
C67705 C67706
C67707
1J8.9 H 8 .2 H I.5 163.9 159.5 168.0 U 8 .3 H A .4 150.2
139.5 155.6 H 6 .2 153.5 153.2 H 6 .1
160.5 154.6 141.0 152.7
155.0 147.4 167.5 154.3 155.5 138.7 154.1 160.6 145.8 161.8 163.0 151.3 170.0 168.4
159.5 156.4
141.4 167.4
153.8 158.8 156.4
169.5 183.4 180.9 161.3 154.4 163.2
149.8 175.9 162.7 164.9 166.8 150.2
168.6 171.4 154.8 163.5 161.2 143.7 176.8 166.9
162.5 141.2 157.4 167.1
153.9 171.3 167.7 161.9
175.8 175.0
170.5 174.6 154.2 180.2
164.1
174.3 166.7
190.1 193.2 198.4 177.2 168.0 170.8 158.0 182.4
175.8 173.5 179.9 153.3 188.9 172.6
178.1 179.2 176.0 137.4 186.7 184.3 181.1 155.0 166.9 180.2 170.2 186.5 187.3 169.9
183.1 193.4 200.7 196.0 157.9 197.6
172.6 178.4 181.1 199.7 200.1 208.4 180.1 176.6 179.8 169.9 180.4 186.7 181.7 183.5
190.4 179.2 177.0 187.9 175.0 155.6 197.1 193.5 181.0 157.8 170.1 182.8 184.5 205.0
195.5 174.5 186.1 207.0 194.0 202.8 164.8 205.4
198.3 186.7 192.5 206.0 207.6 213.7 189.3 182.5 178.8 185.2 183.0
180.9 189.0
*
199.2 184.9 174.8
159.9 207.7 195.2 193.8 167.7 171.8 191.9 195.6 219.2 209.0 183.6 191.5 221.1 204.2 209.3 176.8 202.4
* Dead animal ** * Invalid value - food wasted or spilled.
CQ19S)
(NJ
t--
Individual Food Consumption Oats (g)
______ Wek__________ _L -S_ JL
100 oaa 1-5180
10
189.3 IBS. 188. 201.
*
211.0 188.7 182.2 173.7 179.6
*
175.9 183.
*
19.6
*
18*2.2
*
155.5
*
186.8 162.8
*
189.6 *
215.1 20.9 182.9 190.0 236.3 198.2 20.2 176.3 213.3
192.8 18.5 197.3 198.2
207.5 186.2 177.5 178.7 179.5
*
180.0 181.8
*
186.7
*
177.9
*
159.3 * *
189.1 156.0
186.5 *
196.8 201.6 173.1 188.2 215.3 190.7 206.5 170.2 210.8
188.2
17.1 180.2 200.
*
190.2 182.7 181.5 205.0
187.8 18.3 170.9 172.3
186.8 191.7
172. 183.5
*
**
176.8
178.
177.9 169.9
18*2.9
189.1 *
169.1 ` 182.3 * **
159.2 * *
165. * *
188.3 1*8.7
189.9 1*7.0
179.8 *
189. 198.4 168.5 157.5 185.3 195.0 172.0 162.5 1*9.6
188.
A
206.8 178.0
*
*
207.5
*
171.5
A
193. 177.5 177.2 169.3
*
123.2 179.6 168.7 176.3
*
173.8 ft*
18S .6
128.5 * ft
137.7
187.1 148.7
184.9 *
199. 176.9
* *
205.2 *
173.0 ft
11
190.1 126.0 176.9 194.3
138.2 174.8 165.9 173.1
*
ft ft
167.2
1 5f9t. 6 1 8f1t. 9
134. ft ft
142.1 ft ft
162.8 146.4
ft
190.7 ft ft
198.7 176.7
ft ft
2 0 f3t. 7
164.9 ft
12
200.1 1* 4 .7 185.2
1 9f5t. 8
142.0 176.3 165.2
1 7f8t . 8 ft ft
174.2
16f2t.1 18f5t.3 1 5f6t. 4
ft
150.9 ft ft
186.0 147.9
ft
188.5 ft ft
203.8 179.2
ft ft
198.5 ft
166.1 ft
13
192.8 127.9 176.1
1 9f0t.9
114.8 173.7 155.6
1 7f0t. 5 ft ft
164.8
1 5f5t. 4 1 8f4t. 5
153.3 ft ft
151.2 ft ft
184.5 142.0
ft
183.2 ft ft
197.6 170.6
ft ft
187.4 ft
1 6f7t . 8
HUI 6129- tOQ
Animal Humber
C67671 C6767? C67673 C67674 C6767S C67676 C67677 C67678 C67679 C67680 C6768I C676B2 C67683 C67684 C6768S C67686 C67687 C67688 C67689 C67690 C67691 C6769? C67693 C67694 C67695 C67696 C67697 C67698 C67699 C67700 CS770I C6770? C67703 C67704 C67705 C67706 C67707
Individu! Food Consumption Oata (g)
JL.
ft *
180.8
114.6 *
153.5 ft ft ft ft
165.2 161.8
ft ft ft ft ft ft ft ft ft
174.? ft ft
ft ft
ft ft
169.9 ft ft
203.7 ft
164.5 ft
-li-
ft ft ft
192.7 ft
140.6 ft
159.2 ft ft ft ft
174.2 182.5
ft ft ft ft ft ft. ft ft ft
185.6 ft
ft ft ft
ft ft
182.4 ft ft
212.2 ft
177.5 ft
_______ Meek______
_1L.
J I.
_LS_ J L .
_2fi_
_2L
100 Dom -5180 (Continued)
ft ft ft
192.7 ft
132.2 ft
158.7 ft ft ft ft
167.5 177.6
ft ft ft
ft ft ft ft ft
187.2 ft
ft ft ft
ft ft
175.7 ft ft
217.0 ft
171.9 ft
ft ft ft
196.1 ft
140.4 ft
157.5 ft ft ft ft
162.6 171.0
ft ft ft ft ft ft ft ft ft
189.3 ft
ft
ft
ft
ft ft
170.8 ft ft
201.1 ft
167.5 ft
ft ft ft
204.1 ft
144.1 ft
164.5 ft ft ft ft
174.0 178.4
ft ft ft ft ft ft ft ft ft
189.5 ft
ft
ft
ft
ft ft
183.1 ft ft
198.9 ft
172.5 ft
ft ft ft
201.1 ft
154.3 ft
157.2 ft ft ft ft
173.9 173.4
ft ft ft ft ft ft
ft ft
195.1 ft
ft ft
ft ft ft
182.4 ft
196.7 ft
167.8 ft
ft ft ft
195.5 ft
53.3 ft
154.6 ft ft ft ft
164.7 165.4
ft ft ft ft ft ft ft ft
193.9 ft
ft
ft ft ft ft
177.1 ft
200.1
168.8
ft ft ft
196.4 ft
165.5 ft
159.5 ft ft ft ft
171.6 146.6
ft
ft ft ft ft ft ft ft
193.5 ft
ft
ft ft ft ft
173.9 ft ft
192.9 ft
171.4 ft
"S 6 T 0 0
*"*1
Huifcer
C67708 C67709 C67710 C67711 C67712 C6771J C677H C67715 C67716 C677I7 C67716 C67719 C67720 C67721 C67722 C67723 C67724 C67725
Individui Food Consumption Oats (g)
___________________________________ Weak
_L 2 _L 4 5
JL 8
9
165.6 1*2.6 160.7 157.5 159.0 169.9 138.7 15*. 7
159.* 152.4 164.0 161.3 182.5 154.2 1*9.4
163.0 158.4 1*9.2
175.4 155.6 179.7 176.2 169.8
180.9 135.5 163.7 161.9 162.7 176.5 164.7 195.5 148.0 161.0 173.4 163.9 166.0
196.6 175.8 203.1 178.1 198.5
192.6 1*0.7 177.1 165.4
164.8 193.6 180.0 224.0
171.9 176.3 186.4 191.7 189.0
201.2
170.8 200.4 181.0 197.6
193.4 134.9 193.1 169.6 176.5 199.1
181.8 235.4 171.2 184.0
186.3 197.6 188.9
210.9 181.9 206.5 185.9 183.8 199.9 153.4 200.9 176.1
205.2 194.9 257.0 187.4 176.1 194.3 211.3
100 Dos T-5180 (Continued)
214.5 177.6 205.6 181.9 210.2
ft
153.4 ft
167.7 ft
204.0 191.1 244.2 179.3 180.9 188.0 208.9
ft
220.1 168.2 196.7 178.2 206.5
ft
154.7 ft
164.2 ft
197.0 189.3 238.1 170.6 180.0 183.0 208.0
ft
190.6 118.7 193.4 179.2 207.1
ft
ft ft ft ft
215.7 ft
155.5 158.3 ft ft
160.8 168.0 ft ft
201.6 136.1
ft ft
242.0
145.1 129.6
242.9 ft ft
176.6 177.1
175.9
ft
ft ? ft
10
ft
ft
ft
ft 212.5
ft 160.1
ft 162.1
ft ft ft 243.4 ft ft 172.1 ft ft
11
ft
ft
ft
ft 205.5
ft 159.0
ft 138.4
ft ft ft 244.8 ft ft 173.8 ft ft
12
ft
ft
ft
ft 213.2
ft 158.6
ft 160.7
ft ft ft 240.6 ft ft 170.3 ft ft
13
ft
ft
ft
ft 218.5
ft 157.0
ft 162.0
ft ft ft 236.2 ft ft 174.3 ft
ft
* Ocad animal ** In valid value - food wasted or s p ille d .
HUt 6329-100
A n im i
H *fae r
C67708 C67709 C67710 C47711 CA7712 C67713 C677H C67715 C67716 C67717 C67718 C67719 C67720 C67721 C67722 C67723 C67724 C67725
Indlvlffcjal Food Consuiption Data <g)
_____________________________________ Utek_________________________________________ _ H _ 1S _ 16 17 18 1 20 21
100 oca 1-5180 (Contlnutdl
. . .
. . . . . . . .
139.9 * .
* . . .
. . . . . . . .
181.J *
.
* * * . . .
. . . . . . . .
179.2
.
* * . .
. . . . . . . .
170.1 *
.
* * . ... ... *
... ... ... ... ... ..* ... ...
179.2 173.7 *
...
. . .
. . . . . * . .
171.5 *. .
ITT .4 *
* > Dead anim i ** > In v alid value food wasted or s p ille d .
HUI 6329-100
S6T03
ro
00
Animal Batte
-2- J L
4
C67726 C67727 C67728 C67729 C67730 C6773I C67732 C67733 C67734 C67735
C67736 C67737 C67738 C67739 C67740 C6774I
C67742 C67743 C67744 C67745 C67746 C67747 C67748 C67749 C67750 C677SI C677S2 C67753 C677S4 C67755 C67756 C67757
C677S8 C67759 C67760
IS3.0 154.9 154.6 154.9 154.1 154.9 154.9 152.6 153.0 154.7
152.9 154.3 153.0 153.9 154.9 154.9 153.4 151.9 154.9 154.9 154.9 154.9 154.9 154.9 154.9 154.9 154.9 154.9 154.9 154.9 150.4
154.9 154.9 154.9 152.9
165.0 165.0 165.0 165.0 165.0 165.0 165.0 165.0 165.0 165.0 165.0 165.0 165.0 165.0 165.0 165.0 165.0 165.0 165.0 165.0 165.0 165.0 165.0 165.0 I6S.0 165.0 165 0 165.0 165.0 165.0 162.8 165.0 165.0 165.0
160.8
179.3 179.3 179.3 179.3 178.3 179.3 179.3 179.3 179.3 179.3 179.3 179.3 179.3 179.3 179.3 179.3 179.3 178.6 179.3 179.3 179.3 179.3
179.3 179.3 179.3 179.0 179.3 179.3 179.3 179.3 176.7 179.3
179.3 179.3 166.0
184.6 184.9 184.9 184.9 182.6 184.5 184.9 184.4 180.0 184.9 184.7 184.9 184.9 184.9 184.9 184.9 184.9 170.9 184.9 184.9 184.9 184.9
184.9 184.7 184.9 184.4
184.9 184.5 184.9
175.3
181.2 184.9 184.9 184.9
176.0
*
*
193.7 193.7 190.5 193.7
193.7
182.2
193.7 193.7 179.6 1937 154.4
*
180.0
186.8
193.7 193.7 193 7 193.0
193*.7
193.7 1930 193.5 166.4
183*.4
19*3.7
179.9
Dead animal. 1 Spilled feed.
ro
KO
jS6T00
Individu*1 Food Consumption Osta (9)
JL
Meek
A.
JL
*
190.8 ft* ft
190.8 190.8
ft
174.2 ft
190.8 187.1 163.7 190.8 IS O . 1
ft ft
175.9 175.6
190.8 190.8 190.8 190.8 172.6
ft
190.8 190.0
ft*
167.8
1 7f6t. 0
190.8 ft
ft
0 DO*
ft ft
188.6 188.6 185.2 188.6 188.6
ft
166.2 ft
188.6 177.6 158.0 188.6
1 5f5t. 9 ft
168.9 181.0
ft
1886 188.0 188.6 187.6 163.7
ft
188.6 187.3 184.9 1530 175.1
ft
187.5 ft
170.6
ft ft ft ft
182.5 ft
184.3 ft ft ft
182.5 ft
164.0 ft
16f4t. 0 ft ft
178.4 ft
184.2 183.8 183.6 183.8
ft ft ft ft
182.4 156.9 170.3
ft ft ft
173.7
ft ft ft ft
184.8 ft
185.1 ft ft ft
185.1 ft
165.9 ft
1 7f7t. 3 ft
ft
181.6 ft
185.1 183.6 185.1 184.0
ft ft ft ft
185.1 155 7 176.3
ft ft ft
180.0
JO .
ft ft ft ft
175.8 ft
175.9 ft ft ft
176.1 ft
160.3 ft
1 6f6t. 3 ft
ft
171.9 ft
175.9 174.9 176.1 175.8
ft ft ft ft
176.1 159 3 167.8
ft ft ft
171.8
JLL
ft ft ft ft
172.1 ft
173.1 ft ft ft
172.9 ft
168.9 ft
172.4 ft
ft
ft
173.4 ft
173.0 173.4 173.4 172.5
ft ft ft ft
173.4 160.1 165.9
ft ft ft
160.7
JL.
ft ft ft ft
175.1 ft
177.0 ft ft ft
177.1 ft
158.0 ft
167.3 ft
ft
ft
175.2 ft
177.0 174.9 177.2 177.1
ft ft ft ft
177.2 155.8 164.3
ft ft ft
176.2
J 3L
ft ft ft ft ft* ft
171.3 ft ft ft
171.7 ft
157.5 ft ft ft
ft
ft
168.1 ft
171.2 163.4
ft ft ft ft ft ft
17f1t.7
159.5 ft ft ft ft
HUI 6329-100
Individual food Consumption Oata (g)
Animal
JhmfaSr
C6776I C67762 C67763 C67764 C6776S C67766 C67767 C67768 C67769 C67770
_L
148.8 154.9 154.9 143.9 154.9 154.9 149.2 154.9 154.9 153.0
165.0 165.0
165.0 165.0 165.0 165.0 165.0 165.0 164.9 163.3
JL
179.3 179.3 179.3 179.3 154.7 179.3 179.3 179.3 179.3 166.8
4
184.9 184.9 184.7 184.9 169.5 180.0 184.9 184.9 184.9 169.6
_5_
193 1 179.5 183.9 192.9 193*.7 *
wsL _S_ _L -fi_ 9
0 ppm T-5180 (Continued)
* ft ft ft
ft ft *
190*.8 18f8t.S **
ft
ft 164.7 188.5 153.6
ft ft
184.9 190ft.8
179.3 182ft.5
178.1 *ft
17*7.9 ft
ft *
ft *
_UL
ft ft ft ft ft ft 17f0t.2 ft ft
JJL
ft ft * ft ft ft 167ft.7 ft ft
_LL
ft ft ft ft ft ft 16f3t.2 ft ft
J 3_
ft ft ft ft ft ft 161ft.9 ft ft
* * Dead anima).
** - Spilled feed.
HUI 6329-100
J[&6T00
frOsoj
HWI 6329-100 APPENDIX C Individual Clinical Chemistry Values
221 C019s 6
&o
p (
rNr\o>
0
ANIMAL NUMBER
INDIVIDUAL CLINICAL CHEMISTRV DATA
HALES
WEEK S
PCOAO IU/Q
GROUP: 1 C67461 C6746I C674S6 C67491 C67500 MEAN S.D. N GROUP: 2 C87524 C67S25 C67530 C675S5 C675SI MEAN S.D. N GROUP: 3 C67576 C67S77 C67S91 C67S9S C67599 MEAN S.D. N
DOSE LEVEL: 0 9I 9 t 9 I .5 S
DOSE LEVEL: 1 I 9 8
10 12 9
1.7 5 DOSE LEVEL: 10 11 16 10 17 II 14 3.6 5
DOSAGE UNIT: PPM DOSAGE UNIT: PPM DOSAGE UNIT: PPM
HWI 6329100
ANIMAL NUMBER
INDIVIDUAL CLINICAL CHEMISTRY DATA
MALES
WEEK S
PCOAO IU/Q
OROUP: 4 C87027 CS7631 CS7632 C67663 C8766S MEAN S.D. N OROUP: S C87882 C878I9 C878B0 C87717 C8772S MEAN S.D. N OROUP: 8 C87728 C87733 C67741 C87745 C67782 MEAN S.D. N
DOSE LEVEL 18 21 43 IS 27 24 11.4 S
DOSE LEVEL S8 21 41 43 It 37 14.6 5
DOSE LEVEL 8 1 I 1 t 8 .4 5
100
DOSAOE UNIT: PPM DOSAOE UNIT: PPM DOSAOE UNIT: PPM
HWI 6329100
S6T0.0
no
nCoO
oo
p
rrvo)
ANIMAL NUMBER
INDIVIDUAL CLINICAL CHEMISTRY DATA
MALES
WEEK I
PCOAO IU/G
GROUP: 1
C674S3 C6T4I0 ce riti cern i eenes
MEAN S.D. N
GROUP: 2
CC7S22 C67S31 CI7532 c irs io cerseo
MEAN S.D. N
GROUP: 3
cersri CI75I3 ce rsii ce rsir CI7812
MEAN S.D. N
DOSE LEVEL
e s 7 e r
r IS s
DOSE LEVEL 7 7 e e
7 .1
S
DOSE LEVEL 10 ie 25 14 IS
16 5.5 5
10
DOSAOE UNIT: PPM DOSAGE UNIT: PPM DOSAGE UNIT: PPM
HWI 6321100
ANIMAL NUMBER
INDIVIDUAL CLINICAL CHEMISTRY DATA
MALES
WEEK I
PCOAO IU/G
GROUP: 4 C87034 C97942 CB7944 C07057 C07SB1 MEAN S.D. N GROUP: S CB79I0 C67707 C67709 C67719 C87722 MEAN S.D. N GROUP: C07731 C07734 C07737 C07700 C67789 MEAN S.D. N
DOSE LEVEL: 30 35 32 30 49 15 32 12.2 5
DOSE LEVEL: 100 55 9
107 40 53 54 35.3 5
DOSE LEVEL: 0 < 7 9 7 5 7 1.5 5
DOSAGE UNIT: PPM DOSAGE UNIT: PPM DOSAGE UNIT: PPM
HWI 329100
C01990
ruNoi
ANIMAL NUMBER
INDIVIDUAL CLINICAL CHEMISTRY DATA
MALES
WEEK 14
PCOAO IU/Q
GROUP: 1 C$7460 C$7462 C67473 C674I7 C67501 MEAN S.D. N GROUP: 2 C87S28 C87533 C67S40 C67SS1 C675S3 MEAN S.D. N GROUP: 3 C67S64 C6760I C$7610 C$7611 C67615 MEAN S.D. N
DOSE LEVEL: 0 6 7 S 7 7 $ .6 5
DOSE LEVEL: 1 11 7 6 7 I t 1$ S
DOSE LEVEL: 10 t
13 $
10
s 10 2.1 5
DOSAGE UNIT: PPM DOSAGE UNIT: PPM DOSAGE UNIT. PPM
HWI $328100
66T00
orItMo
ANIMAL NUMBER
INDIVIDUAL CLINICAL CHEMISTRY DATA
HALES
WEEK 14
PCOAO IU/Q
GROUP: 4 C67616 C6T623 C67643 C676S2 C67653 MEAN S.D. N GROUP: S C67671 C67672 C676B1 C67700 C67714 MEAN S.D. N GROUP: 6 C67730 C6773I C67746 C67754 C87767 MEAN S.D. N
DOSE LEVEL: 30 11 15 16 10 II 14 3.4 5
DOSE LEVEL: 100 16 14
23 17
...4.5
5 DOSE LEVEL: 0
7 6 5 5 4
51 1
S
DOSAGE UNIT: PPM DOSAGE UNIT. PPM DOSAGE UNIT: PPM
HWI 6329100
1?66T03
iNvj
ANIMAL NUMBER
INDIVIDUAL CLINICAL CHEMISTRY DATA
MALES
WEEK 22
PCOAO IU/G
GROUP: 1 C874S3 C674S6 C674I3 C674B0 C67486 MEAN SO. N GROUP: 2 C67S0I C87518 C87S34 C87538 C67543 MEAN S.D. N GROUP: 3 C67567 C67575 C67579 C875I9 C67S93 MEAN S.D. N
DOSE LEVEL 8 7 7 7 8 7 .7 S
DOSE LEVEL 7 7
10 6 8 7 1.6 5
DOSE LEVEL 8 7 7 7 S 7 1.1 5
10
DOSAGE UNIT: PPM DOSAGE UNIT: PPM DOSAGE UNIT: PPM
HWI 6329100
C0199*
NrC Do
e o
tj) ff\OJ
ANIMAL NUMBER
INDIVIDUAL CLINICAL CHEMISTRY DATA
MALES
WEEK 22
PCOAO IU/Q
GROUP: 4 C67828 C6762I C67837 C67664 C67870 MEAN S.D. N GROUP: 5 C87874 C6767I C878I3 C67694 C67701 MEAN SO. N
DOSE LEVEL 6 7 8 7 7 11 S
DOSE LEVEL 7 6 7 7 7 7 .4 S
100
DOSAGE UNIT: PPM DOSAGE UNIT: PPM
HWI 6329100
