Document e5doz4VeL2aMxdo3rZBkk5mrq

BIODEGRADATION (OECD 301 D) TEST SUBSTANCE Identity:A mixturecontainingperfluorooctanesulfonatweh,ich may alsobe referredtoas PFOS, FC-95, or as a component ofFC-780B. (1Octanesulfoniaccid)(CAS # 2795-39-3). Remarks: The 3M productionlotnumber was 2. The testsample isFC780B. Currentinformationindicatesitisa mixtureof0.58% PFOS, 14% diethyleneglycolmonobutyl ether,76.7% water,5.5% urea,1.17% Hydroxy foamer, 1.15% sodium octylsulfate0,.8% polyoxyethylenemonooctylphenyl ether,0.05% sodium laurylsulfatea,nd 0.05% benzotriazole. The followingsummaty applies toa mixture with incompletely characterizedconcentrations of impurities.Data does not accurately reflectdegradation potentialof the fluorochemical component of the testsample. METHOD Method: OECD Closed BottleTest,OECD Guideline301 D and C 117/79/GER Test type: Ready Biodegradability GLP: No Year Completed: 1980 Analyticalmonitoring: Dissolvedoxygen depletion Statisticamlethods: Resultswere determinedby calculationfthe % BOD/COD overtime Test organism source: Non-pasturizedfieldsoilobtainedfrom 3M Agriculturaglreenhouse, St.Paul,MN Test condition: Dilutionwater: Deionizedwater and distillweadter. Reference and testsolutionpreparation: The dilutiownater was prepared usingHach BOD SlurryPillows.NitrificatiionnhibitoHra,ch Forumia 2533, was added to replicatdeilutionosfblanksand samples atthe recommended concentrationof0.1g/300 mL. Test vessels: Not given. Number of concentrations: 1 plus2 referencesubstances,and blank,allinduplicate. Temperature: 200C Totalsuspended solidsand pH on day oftesting: Not given. Element Basis: Decrease inoxygen depletioncompared tothe blanks. RESULTS Nominal concentrations: Blank control,LAS and sodium dodecyl sulfate (referencesubstances),and testmaterial. Element values: 5-Day BOD/COD: 21% 15-Day BOD/COD: 78% 28-Day BOD/COD: 72% Remarks: Testingwas conducted on the mixtureas described inthe Test Substance Remarks field.The values reportedapplytothatmixtureand not the fluorochemicalproportionalone. CONCLUSIONS The testsubstance % BOD/COD was 72 after28 days. The % BOD/COD withnitrificatiinohnibitewdas 83 after28 days. Submitter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,Minnesota,55133 DATA QUALITY - ReliabilitKyl:imischranking= 3. Study lacksdescriptioannd recordsofthe method followed.The inoculumisnotpropedy characterized.The sample puritywas notproperlycharacterizedand the studylacksanalytical confirmationofthe amount offluorochemicalproportioninthe solution. REFERENCES The studywas conducted by the3M Company, EnvironmentalLaboratory,St. Paul,MN, Lab Request number 6211 S, 1981. OTHER Last changed: 6/28/00 (o2, Test Test Gtiideline C 117/79/r,---R Guideline for the Closed Bottle Test acc. to Fisclier Date of last revision: August 27, 1979 Lovel.I test for ready biodegradability 1. Prerequisites It has to be known whether the test material in tlio concen tration range employed in.tho test mg/1). If it *is not,spoc3.al measures such as of soltibilizing agents, ultrasound dispersion have to be applied. is soluble (2 - 10 the addition etc may 2. Guidance Information Knowlecl-e of the bacterial toxicity or inhibitory properties ofothe test material is not unequivocally required but constitutes useful info.-mation. 3. QualifvinZ Statements The test is applicable for the biodegradability evaluation of or[;.-iniccoiiipoun@.b7u.ts not for mixtures thereof. %lost experience has been gathered with water soluble corpouzids, however, the test.lends itself, at least in principle, V/ also to tticbiodegradability evaluation of volatile anci iiisoluble.coml)ounds. August 27, 1979 OECD Chemicals Testing Programme Test Guideline for the Closed Bottle Test acc. to Fischer 0. The test Procedure is based on a paper, by X.Fischer. P.Gerike, and R. Schmid in Wasser- und Ab-ea.sserforschung 99 -118 (1974). 1. Method 1.1 Introduction: Purpose, scope.,relevance, and application of test and explanatl:onof limits. The purpose of the method is the n-easuren-entof the biodcC4-adability of organic com pounds in an aerobic, aqueous mediu.-iat a test'concentration of 2 (standard concentration) to 10 mg/l of active material. r-lost data elaborated with 7his test pertain to water-soluble compoundzs however, volatile.compounds a4d those of low water solubility may also.,at least in principle, be tested. 1.2 Defi-nitons and units 1.2.1 Definition of biodegradability mg 02 / mg AS tA) % BODT --------- x 100 BODT BOD/CCD= mg 0 2 / mg AS mg COD/mr, AS x 100 via Iiolko.apwo SAMPLE DESCRIPTION File: Page No. ENVIRONMENTAL ENGR. LAB - WORK SHEET Date: eO Analyst: Hrs.: v /)-e-eo@p,@ ebb e4l4 Pz,@ FdRM41i,19 Z'3,oL, ,v. Reviewed by: 3m ENVIROMIENTAL LABORATOITY Ref.: Form Shoot Lab request no-: 6211S Date of startof test- 10-31-80 Test / standardmaterial: AFFF- BOOT of test material: mg 02/mg for the Closed bottle Test ,OV "7(6 '1Analyst- W. A. ScheiI Theoreticaltestconc.: il7l. AS COD of test material: (AS - 0.34.,o.t/ ag AS OECD, Tokyo,12-01-79, C 117/79/GER I actAiSv,e substance) Temp. of the dilution water after aeration:--- 293 K 20 oc) of the distilled (dolonized) water aifter :.2r-a-rt-i'on and standing before the start of test:- 8. 9S Mg 02/1 Dissolved Oxygen analysis: oxygen electrode method calibrated oasinst the Winkler method lnoculum: Unfertilized garden soil A-. 22 DETERMINATIONS-:CULTURE MEDIUM Flask no. mineral nutrient solution without e7ia1-and wt@i:'ho"u*ttin.ul,... I - 2 3 lvk fcfoanotn rol Mineral nutrient solution without tb"etst material with lftoev 1 2 3 4 mean blank Analyses og 02/1 AFTER X DAYS 0 5 is 26 % - Cl CI Cl, C3 C4 C2 ... cm n 1 2 03 54 8, $I # 62 n g.i 8.65 8.50 - 9.101 8-70 8.55 9. 101 8-75 8-5 5 9. I!t 8-7(@'!8.50* 9.101 8.70 - 9.101 8.75 9.101 8.70 9.151 8.70 9. 121 8.75 8.53 8.50 8.50 8.55 8.50* 9.12 8.72 8.52 6.90 - 7-20 6.85 8.25* 6.98 6.8o 6.90 7.10 8.20* I 6.93 Minaret nutrient s,:ISu,tio"ntowri7tha7 ith and lnmulum. I A -- 2 A --- A2 3 A t At + A An material 4.60 (A@-L 10 1-7.(ooV,-1@lc* 2 2.0 *Values not included in the mean values; these dilutions conta in nitrificat ion inh ib itor *See note above. B: (6-DEPLETIONS (mg 600/1) AFieR X DAYS: BOOK - (% - otx) - (% - mbx)* This difference Is Important as a check for the validity of the test (see 1.6.3.1.) MODIFIED FOOtMLAt soox (Mto. 0 tit The radifle4 formula uses the measured Initial a, conc. of the test solutions and blanks In place of C; EVALUATION* 'I,OWT ag DWX/I . too OR % goo /COD 09 BOOK/I Ica x mg AS/I - cw mg AS/I BOOT after days s 00 x /I after x days Is 28 'I.BOOT x ,/AW/COD I 2,1 @-.6@7Z S' 3.9 -A 3.51 4 /C- y@ COMMENTS., -7L % 3M E14VIRONMENTAL LABOITATORY For* Shoot for the Closed bottle Test Lab req"st no.: 621]S Date of start of test: 10-31-80 Analyst: W. A. Schai I Test / standard material: DobuvL '-COD@UM WrTheoretical test conc.: BOOT of tes t motor Ia I ma 02/m AS Temp. of the dilution water after aeration: COD of test material: 0 293 K 2 0 Oc) Ref.: OECD, Tokyo,12-01-79, C 117/79/GER 2-40 ma AS/] (AS - active substance) mg (@i/ma AS 0 -conc. of the distilled (deionized) water ftor 2 &eration and standing before the start of tesat: 8.9S mg (ii/I Dissolved Oxy"n analysis: Oxygen electrode method calibrated sa@inst the Winkler method lnorulum.--Unfortilized garden $oil A: 22 DETERM114ATIONS, CULTURE MEDIUM Mineral nutrient solution without test material and without lnmulum. Mineral nutrient solution without t st t not@ra@i b: with lnoculum. Flask no. I 2 3 4* most % can ral 2 3 4 mean blank Analyses Cl C-A C3 C4 C2 ... Cn 2 113 S4 81 * 02 ... On n 09 02/1 AFTER X DAYS 0 5 IS 90 1 8.65 8.50 91 0 9.10 8.70 8.75 8.55 8.55 9. 10 r 8.70t 8.5 0* 9. io 8.70 8.53 9.10 8.75 8.50 9.10 8.701 8.50 9.15 8.70 8.55 9. 1o@, 8.751- 8.5 0* 9.12 -8.72 8.52 28 6.90 7.20 6.85 8.25* 6.98 6.80 6.90 7.10--.8.20* 6.93 *Va Iuss not in-. cluded in the mean values; these di1ut ions conta in nitrificat ion inh ib itor Mineral nutrient solution w-l-th atnedst material with lnoculum. I A 2 A 2 3 A 3 4 A4 ot Al + An to t mastnerial qlio 16,30 -5,'70 91D.5-*l 30-A ijs- 9,/o eo .3,46z) $0 *See note above. 3. 13!; 6: C@, DEPLETIONS (mg BOO/1) AFTER X 0AYSi Boox - (mo M,X) - (m, - N )* x !his difference Important as a check for he validity of "he test (s*e 1.6.3.1.3 Cl EVALUATION: MMIFIED FOItMLAt BODX. (o 0.0 t tx (*be- 1% The modified formula uses the measured Initial 02 c@.. of the test solutions and blanks in place of mo. X BODT - mg BODX /I . 100 mg AS/I BOOT after BODT x -.I-- /Soo/cw OR do" I& '7zl % BOD /COD x 2]R '76 09 BWX/l [Do mg AS,'I 0"9 ODX/I after X days 5 1 Is 1 28 00 CaXl COMMENT$O 3M ENVIRONMENTAL LABORATORY Form Sheet for the Closed bottle Test 62]]S Lab request no.: Date of start of test: 10-31-80 Analyst: W A. SChei1 Test / standard material.-I-ASREFEff-AtMEAP-IIF Theoretical test conc.- BODT of test material: .1M)Q 02/,g AS Temp. of the dilution water after aeration: COD of test 293 K terl.): Z 20 oc Ref.: OECD, Tokyo,12-01-79, C 117/79/GER ma AS/I (AS - active substance) mg 02/ mg AS ' of the distilled (delonized)water after 0.2.;act-lcon and standingbefore the startof test:_8.9s - mq 0!1_I/ Dissolved Oxygen analysis: Oxygen electrode method calibrated aaainst the Winkler method inorulum: Unfertilized garden soil A: 02 DETERMINATIONS- CULTURE MEDIUM Mineral nutrient solution without test mateI rial and without rkm u Ium. Mineral nutrient solutionw II!hIou t t sttw "ter b: ith lnacu lum. Flask no. Analyses I 2I 3 16* mean % control 1 2 3 4 mean blank Cl C, C3 c4 * C, + C2 ... c ftn 01 02 83 B4 9 1 + 92 on n mg 02/1 AFTER X DAYS 0 5 is 9. 1 8.65 8.50 9.10 8.70 8.55 g.lo 8.75 8.55 g.lo 8.7(@, 8.50* 9.10 8.70 8.53 9.10 8.751 8.50 9.10 8-70 8.50 9.15 8.70 8.55 9.10, 8.751- 8.50* 9.12 8.72 8.52 28 6.90 7.20 6.85 8.25* 6.98 6.80 6.90 8.2D* 6.93 *Va Iues not in- cluded *ir the mean values; these di- lutions contain nitrification inhibitor Mineral nutrient solution with test water=al and with inoculum. I A I ?,/o 9.4sl 4,0l,o5- q3.,.-7.0@r 2 A2 3 A3 4 A4 os@% L3,(OO-A (0,.50 or* *See not a above. on t A, + A 2 **'An It., n CIO 8: 0!1DEPLETIONS (mg 000/1) AFi'EltX-DAYS* BODX - (m - mtx) - (mo - % )* 0 x This difference Important as a check for the validity of tIL test (see C: EVALUATION: X BODT - mg SOOX /I 100 AS/I 'I.IWTX r -law-/COD @@-80DICor) COMME14TS: * BOOT - &ft* days s I Iit o-91 1q3 St? MWIFIEO F43RHULA: BODX at.) The modified formula uses the measured lnltl&l 02 conc. of the test solutions &W blanks In place of mg. Olt % 900 x/COD 1 29t 1 *9 I i@6- s eq saox/I 100 AS/i - rup mg loox /I after x d"s 51 IS 1 28 DSJ :t.4 Irk 1441" o-O -r 2 I.AS /0 Ai@( US-EPA FONA 545S- A ANALYSIS FORM FILE DATE S14EET -&,t ol7--#@ -re <-,2: @OLLK L 1014S a c D F G L AEF_F-A 2 7.o-.,c&rev 3 L)_L W" .4@@ dj,.:td 9, 7.3 o,,O .1.,@Io (.5;2.-?o 4 5 6 7 FF-E- r3 o @--t 'Lt@ 10 11 12 13 '75' 170,0 -70,6 3.6.T -7,.02 '7. 1 14 AFEE -C 1.0 I"t -4ztr@A-44@ 16 I.o-t a,,izk 117 is 19 D,,dg 20 21 _L2 IAII@ZZ 23 24 -76 -7 -)a 1yo -v- ldo-l1o--1;oll 17110-7f.D 4.5-< 6.300 27 36 28 29 30 31 33 34 35 37 38 39 40 .DL') AL 43 -i4 45 l,f3 izo S-0 91 z 0 1Vol) - IL40- jr 9,5"06 '70 91/14-17,70CS-C'7,2-()i v7 9-90 '?- it4i+ttkT,DIR FORM 10620-8-PWO SAMPLE DESCRIPTION ENVIRONMENTAL File: Page No. ENGR. LAB - WORK SHEET 2-if J fXNt Date: 1() 90 AnalystL:J,'Q- Hrs.: -ofaz 61ap 4t @7 yi 11 o iD 00 2,00 9,lo /0 Met D'st@UP-A) I bell 2z- T,00 K6 w@@t 7 ,-7o 3e) o -rk@s 41AAA-0. L"6 qm-^a ,A:t. .'I. o./.1a30tbX R eviewed by: FORM I 0620-8-PWO SAMPLE DESCRIPTION ENVIRONMENTAL File-:-.JlaNgoe. CO ENGR. LAB - WORK SHEET Date: /0-3/-go Analyst:I-J,tf-, Hrs.: .of L/ I.X 7/0 10 IV A FFF -4 A@F FF C ttj U 00 j,5,q 15'd y 0.2-0 0 41 4Q 4 A nall-A@ 10- 6,o 510 4,o 9 2- g E2-9' A S il IA) mw KebIX)0I5I10@-il7k I?CF6,ecA<C- ('719/D,a@&71 ,P,V,0&4a v tRiA.16i-te hl31g Reviewebdy: