Document dQn9ODgEavVBNqXRBeJBQm38q

AR R86 _ 0785 ACUTE TOXICITY TO INVERTEBRATES (FIDDLER CRAB) IesTsuBsTA 000NCE Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-206 or L3243. (1-Octanesulfonic acid) (CAS# 2795-39-3). Remarks: The 3M production lot number was not noted. The test sample is FC-206, identified by the laboratory as "Sample C". Current information indicates itis a mixture of 0.67% PFOS, 17.5% diethylene glycol butyl ether, 78.91% water, 1.33% Sultone foamer, 1% sodium octyl sulfate, 0.04% sodium lauryl sulfate, 0.5% polyoxyethylene monooctylpheny! ether, and 0.05% benzotriazole. - The following summary applies to a mixture with incompletely characterized concentrations of impurities. Data may not accurately reflect toxicity of the fluorochemical component of the test sample. METHOD: Method: Standard Methods (APHA) 1971 Edition Type: Acute static GLP: No Year completed: 1974 Species: Uca pugilator Supplier: Field collected locally in MA. Analytical monitoring: Temperature, and dissolved oxygen. Exposure period: 96-hours Statistical methods: TLs (median tolerance limit) values calculated using a linear regression equation. Test organism age: Juveniles. Length and weight: Average carapace width = 20 mm Loading: Not given. - Pretreatment: None Test conditions: Dilution water: Synthetic sea water Dilution water chemistry: oH 80 Salinity 25 ppt Lighting: Not given. Stock and test solution preparation: Not given. Concentrations dosing rate: Once Stability of the test chemical solutions: Not noted Exposure vessels: 5 gallon glass vessels containing 15L of test solution. Numofrbeple icatres: 1 001489 NNuummbbeerrooff organisms per replicate: 10 concentrations: seven plus a blank control Water chemistry during the study: `Temperature range (0-96 hours): 20 1 C Dissolved oxygen range (0-96 hours): 4.28.6 mgiL Reswts 00000 Nominal concentrations: 6500, and 10,000 mg/L Blank control, 2100, 2400, 2800, 3700, 4800, Element values:96-hourTLsp = 3260 (2760 -- 3860) mg/L 96-hour NOEL = 2400 mg/L Element values based on nominal concentrations. SRuebmsatraknsc:e TReesmtianrgkwsafsielcdo. nTduhcetveadluoenstrheepmoirxtteudraepapslydetosctrhiatbemdixitnutrheeaTnedstnot the fluorochemical proportion alone. conclusions 000 The FC-206 96-hour LCs for fiddler crab was determined to be 3260 mg/L with a 95% confidence interval of 2760 to 3860 observed effect level (NOEL) was 2400 mg. mg/L. The 96-hour no Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 p0 atAQuA0 TY 00000 Reliability: Klimisch ranking 2. Testing meets the criteria for qualitytesting. However, the sample purity was not properly characterized and the study lacks analytical confirmation of the amount of fluorochemical proportion in the solution. - REFERENCES 00000 Test was conducted by Bionomics, EG&G, Inc., of Wareham, MA at the request of the 3M Company, St. Paul, MN, 1974. OTHER _ Last changed: 6/28/00 een. 001490 LTio ate ol Lo - ) Dogon bom a Selle enleakidror, Stsuinq bw 97 onbdue femls Cor) vobtand? i BIOASSAY REPORT SUBMITTED TO 3 M COMPANY SAINT PAUL, MINNESOTA | Wed tv crsbaw C2) ft ] Sean 4 do cy -- st ll | i ] ACUTE TOXICITY OF SAMPLE C TO MUMMICHOG (Fundulus heteroclitus), GRASS SHRIMP (Palaemonetes vulgaris), AND FIDDLER CRAB (Uca | pugilator). { . J BEiGon&oGmi,cs Inc. |1 W7a9r0ehMaami,n SMatsrseaecthusetts ' May, 1974 } 001491 METHODS AND MATERIALS 0 These investigations were performed at the aquatic toxicology laboratory of Bionomics, E G & G, Inc., Wareham, Massachusetts. The susceptibility of mummichog (Fundulus heteroclitus), grass shrimp (Palaemonetes vulgaris), and fiddler crab (Uca pugilator) to Sample ! C, a brown liquid, tested as 100% active, was evaluated | under static bioassay conditions for a 96 hour period. The results were reported as the medign tolerance | limit (TLsy), the concentration of the sample in water causing 50 percent mortality after 24 and 96 ' hours. The predictedTLgg value and its 95% confidence intervals were arrived at by converting the concentrations D tested and the corresponding observed percent mortalities. to logs and probits, respectively. These values were then used to calculate a linear regression equation. | Test procedures for the static bioassays are those described for Fish Bioassay Procedures in the 1971 | edition of Standard Methods (APHA). The mummichog, _ grass shrimp, and fiddler crabs used in these tests ) | were collected locally by laboratory personnel. The mummichog had a mean weight and length of 1.1 g and 35 mm,respectively. The grass shrimp had a mean | length of 30 mm. The fiddler crabs had aneg Fhpace me ) width of 20 mm. 001492 So 3 | i | ] | | | J) | | 1 1 | | | 2D | | Page two The test species were observed in the laboratory hatchery facility for at least 30 days prior to testing. These species were acclimated over a 10 day period,prior to testingt,o laboratory test diluent water quality and temperature. During that period mortality in the test population was<3% and these animals were judged to be in excellent physical condition. `The bioassays were conducted in 5 gallon glass vessels containing 15 1 of water at 20C (* 1.0), "and there was a single introduction of the sample into each test vessel. aerated. The test containers were not The test diluent consisted of synthetic sea water! with a salinity of 25 /oo, a pH of 8,0 and a temperature of 20 (* 1.0). Dissolved oxygen levels for the tests ranged from 8.6 at the beginning of the tests to 4.2 at the end of testing. Ten specimens vere introduced into each test vessel 24 hours prior to the start of the assay. = _ RESULTS The predictedTLsg values and 95% confidence intervals are presented in Table 1. Table 2 presents a summary Laroche, G. E. Eisler, and C. R. Tarzwell, 1970. Bioassay Procedures for 0il and Oil Dispersant Toxicity Evaluations. J. W. Poll. Cont. Fed. 42 (11): 1982-1989. oo 601493 ud 3 Page three of observed mortality for each individual concentration after 24 and 96 hours of exposure. The mortality syndrome in all tests 'was similar. Themummichog and grass shrimp generally became dark and lethargic, lost equilibrium, and expired. The fiddler crabs became lethargic before expiring. 001434 5. . : SUBMITTED BY: 3 Bionomics . E79G0 M&aGi,n ISntcr.eet Wareham, Massachusetts May, 1974 PREPARED BY: APPROVED BY: i | ' 1 i Robert E. Bentley Aquatic Biologist 7 Bevier Hasbrouck Sleight, IIT Goi Nsbopurds Shopbty DFirreeschtwoart,er Programs -- 001495 Sv Table 1 -- Acute toxicity of Sample C to mummichog (Fundulus hl heteroclitus), grass shrimp" (Palaemonetes vulgaris), ar fiddler crab (Uca pugilator). These data are based on results of bioassays conducted at the fish toxicology of Bionomics, E G & G, Inc., Wareham, Massachusetts. | | Species `mummichog |. grass shrimp Commi | Emieri fiddler crab : TL - milligram/liter ZiBew 98 Bow >1,800 <2,400 51,0000 >10,000 1,820(1,360-2,4200% (280(230-342) = 3,260(2,760-3,860) No Effect (mLge/v1e)l 1,400 LC 180 2,400 ) '. ii bio | I | 2pssays conducted at 20C ( 1.0), mean weight of the mummichog 1. Ppssays conducted at 20C ( 1.0), mean length of the grass shrimp 30 mm. Assays conducted at 20C ( 1.0), mean carapace width of the idd crabs 20 ma. 95% confidence intervals. ~ ) a caddie pets th Bd Ald an J 001496 Table 2 -- Concentrations tested and corresponding observed ) percent mortalities of mumichog (Fundulus heteroclitus), grass shrimp (Palaemonetes vulgaris), and fiddler crab (Uca pugilator) after 24 and 96 hours of exposure to Sample C. || Species _-- `mumni.chog . ) Conc(emgn/t1r)ation 3,200 2 2,400 1,800 : 1,400 1,000 control 2% mohrtoaulrity 98obe servr ed 100 100 100 100 0 30 0 0 [J 0 0 0 grass shrimp | | | 1,000 420 320 20 _ 180 140 control 0 100 0 100 0 100 0 40 0 20 0 0 0 o J 1497 3 Table 2 -- Continued Species - fiddler crab Con(cmegn/tL)ration oo "10,000 6,500 4,900 3,700 2,800 2,400 2,100 control py% mortality observed 7TM 100 100 0 100 0 100 = 0 70 0 30 : 0 0 0 0 0 0 J 001498