Document bayKvdxZyLoB5kRyXL4JV3p01

AR226-2760 FOR DU PONT USE ONLY E. 1. du jgjCjoxi.cologv8and^Induatria1 Medicine (see page * for composition) "bmittdby PomerTroducts Wanlngton Works 'u a Date rs of rHisr Completion: ^ lrUTMTTM ^ TRODUCTIOM to decermine ^ h e r the teat sample caJ** ss-s sss-issa'-Sa S e e s^iiir^nnot produce ^^ ^essential amino acid. Bsence of test sample indicates wn trains of bacteria. " 8 - these ----------- ETHODS ?ne,,rDutation_M223LZJi~ii'~2HTM-- " f^^{jjr:fbsence of . r . t - u r --- Tb* aay in^ST Mt:tlod described by <" l *! nrs 2SSl.jr ^ - K S S lS 1 ? 20 i r x l i . - r s-'tScSS*-* - w s s w ^ ~d. of avis minimal agar. Treatment^ g le/t6p agar and pouring tne m- j.S ml ot s-y ml* to "" SCThfc 3-y ml* contained pet ad; "* ml o le> d,,i,,tloute.d with u.*- <'*" ' itr.L. 5 micromoles glucose- -rtlsun nhosDhate (from a 7" ". t*4*m>icro8mo1le"sNaSurp a*"f iid mlc?ro,m.out V ^ sodlum phosphate solution at p * - 1 Company Sanitized. Does notcontainTSCACBt y The S-9 w,,. th. 9,000 x g aupernataac o li.er homogenate U go 0.15 H KCU. The Uver. were obtained from 8 to 9 oeek old onle Charle. Rl.er CO cats given 500 og of A r n c U r 1254/kg five day. before aaorlflce. The revertaut colonies were counted after the places were Incubated at 37 L for ^ " "positive (known mutagens) and negative (solvent) controls were included *-- ;;epc^ alTheScvtotoxiclty of the test sample In the presence and absence of an H ^ c S e f ^ ^ r i S JtL ^ r i f C" SS U n t w i n e on. sslss r r j r t t s r x r z z ^ Tp ^ e . CoScencratlon, of teat aaople that were non.oxlc and, if po.alble. test 222$ not change the volume of solvent. - a K If * Sp0t Thetplate aa.ay may not be the boat method to cant Hguld camplea ttat vaporize appreciably ban thla protocol la 4` in a close ' system is performed on all nonnqueous liquids to aid in identifying S p i e s with volatile mutagen., components. These samples are tested by different protocols. ,,,ro 10a har-eria fin o before mixing. All components used for this assay were identical to the ones used in the plate incorporation assay. ., t sterile disk saturated with test compound was placed in the center of bag and incubated for 4 hours at 37 C. Use of)Data:iate As8ay . Solids and Nonvolatile Liquids is 2) ? r ft h T s dpoifteit r U i t i v e ! a X S Plate Incorporation Assay Solids and Nonvolatile Liquids is performed with only the strains a conditions in which mutagenic activity was suggested in the spot I Z t . T c o m S l e S assay ?. performed if mutagenic activity is observed in the limited assay. aaaau,,A 3) If the limited assay is negative, the test sample Is aasaye according to the plate assay protocols for testing gases and volatile 1laiiids . o 2 C M ^ r a n B t a K f . Do. nol contain TSCACBI  QODflUtaRoil au as a nonmutagen when, test sample la classified habllity la grater than 0.05 th ^ nmnbers of revertants studled are not greater A- trs'toiv^t ..1 AND " " `rioo. of a - M .t pi . __ *.tt tMt 1 classified as a tags Ke! ^ ,,,,ertane. at ' habllity 1 I"" *" 0'`'1 1000 stodled ate aol gteatar " - 1 f - - - ^ r r ^ a t control* AND 8. the P ^hafbljli^tyLloa I6t*h*. tnhuamnber'ofl 'te^vrettatanntts. laad lnctsaslns correlati sample. concentrations of the test sampr de.,lt with on v russified by the above criteria ,,t samples that canaot be cl. ^ ,,,,liable Itr case-by-case basis. ^ q fchft statistical Mutagenicity in the A detailed descrip ^ atls,lcal Evaluation of t g|rA^eneslst The nd R. D> Snee. ' bury Report. -Mammalia^ HO/RGPRT system. g J g ^ d T A T w . turation of. ^ - S p r i n g Harbor Laboratory, IcElheny), P Z0J' _________ ------------ - eln , and V. K. Yorh. _____ -3 tscacbi Comply SanW*ed. Doe# not conta'10 ABBREVIATIONS AND DEFINITIONS . S*. ***,, Q-aminoacridlne concentration of test chemical la listed as 0 . "he" che Toxicity indicated b, decreased density of the b a c k g r o u n d ^ %'d.**^rsn-^" cuEur::irSti^^otoxicit, effirr t l me ^ T ^ t e s t 10 . dosed system os performed ood oo indication of mutagenic activity was observed. , , u n I aBj jv for strains The mutagenesis data are listed in Tables i, n . * icoc *t*a is*i7 TA 98'and TA 100, respectively* ^ 'i of the revertant frequencies obtained in the p r e s e n ^ ^ e d S 2 l .bowed no strains r e r t r e i ' ^ l r ^ o r ^ ^ n n T d 1^ rest chemical was not mutagenic. crlvnrlnn system. Tberdfora. tbs z 3 > , k rztested in & s rz ts ^ e .*i -- c< " these strains of bacteriae __ Composition: ^jj Contaminants: April I*. Hfarst Biologist JVLH:vl Report No. 1072~d0 Date Issued: January Approved by: David F. Erahn Manager, Genetic Toxicology Division Study Director lVBl - A- Sanitized. D oe. not contain T SC A C B I Company TABLE I TYpuTMimlllM STRAIN TA_I535 MUTAGENIC a ct ivit y th salmonella WITHOUT ACTIVATION Compound Concentration Cus/Ei! 13,783 H M 0 100 1500000 5000 10,000 HNNG (Duplicate Platea,) Bavertanta Per Plat Trial 2 " Trial 1 30, 38 22, 21 34, 26 31, 36 24, 20 19, 29 26, 23 37, 31 25, 26 40, 37 28, 22 40, 35 2694, 3019 2243, 3094 ottk activation v Compound 13,783 W Concentration ... Cug/plat- 0 100 500 1000 5000 10,000 ` 2AA 10 aborra is Per Plate (Duplicate Plr.tesl Trial 2 Trial 1 73, 37 44, 45 44, 54 46, 45 46, 54 38, 56 35, 29 41, 22 27, 27 35, 28 31, 32 25, 40 510, 567 798, 747 tscacbi - 5 Sanitized. Doe* not c o n tain Compaq TABLE I I . MUTAGENIC ACTIVITY IN SALMONELLA WPHIMURIM_SmlM TA .1537 WITHOUT ACTIVATION Compound 13,783 Concentration Cua/plate) o 100 500 1000 5000 10,000 9AAc 50 . 17. 7 10, 7 *2, 4 7, 5 8. 11 15. 11 1081, 944 10, 13 14, 6 10, C oc. 5, 8777 1283, 1591 WITH ACTIVATION Compound Concentration Ou r /plate)_ l8,7dJ n tr 0 100 500 1000 3000 10,000 2AA 10 Rpvertants Per Plate (Duplicate^lates) Trial 1 1UL 11, 4 4, 9 12, 5 16, 8 11 9 11, 8 H. a 8, 8 15, 6 8. 5 9, 12 9, 12 440, 72 583, 821 O / Ccmpai*y SanifizesL Does not contain TSCACB1 -TM-c u sb sl s TABLE I I I Compound 13,783 2NF Concentration Onfl/platej 0 100 500 1000 5000 10,000 25 Bouertanta Per^Plgte fn..pHrate Plategj. TrialT Trial 1 41, 35 37, 37 32, 32 33, 30 28, 21 23, 24 25, 24 26, 33 38, 20 22, 23 24,* 26 21, 21 2016, 2647 2895, 2820 WITHACTIVATION Compound 13,783 Concentration 0 100 500 100 5000 10,000 10 2AA RevertantsJ^r_Plate <T>..piirate Plates) Trial 3 Trial 2 46, 43 48, 35 36, 51 48, 37 30, 43 .42, 63 51, 42 53, 50 44, 43 41, 39 48, 37 45, 44 3619, 3648 1838, 2395 `J i i i - 7 Company Sanitized. D oes not contain T SC A C B I TABLE IV activity salmomella nranwRiUH s m i n t a .100 UTTHOUT activation Compound 13,783 t* Concentration 0 100 500 1000 5000 10,000 mnng 4 Revertants Per Plate (duplicate Plates) Trial 1 Trial 2 121, 116 146, 161 103, 121 115, 156 115, 100 120, 121 114, 103 101, l'O 88, 100 85, 117 92, 90 49(T), 86 (T) 2493, 2564 3982, 3501 WITH ACTIVATION Compound Concentration (ng/plate) 13,783 0 100 500 1000 5000 10,000 2AA 5 Reyertante Per Plate (Duplicate Plates) Trial 1 Trial 2 135, 158 144, 147 145, 171 132, 157 140, 155 143, 168 163, 154 147, 168' 156, 152 156, 149 129, 132 144, 131 3125, 3169 2491, 2565 -8 TSCA CB1 o e s not contain