Document bBXQ2qYp7rvwv182DzpmEo6q3

07A f L Z Z G - S/ TOXICITY TO AQUATIC PLANTS TEST SUBSTANCE________________________________________________ Identity: A mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-203CE. (1Octanesulfonic acid) (CAS # 2795-39-3). Remarks: The 3M production lot was not noted. The test sample is FC203CE. Current information indicates it is a mixture of 1.84% PFOS, 30% diethylene glycol butyl ether, 60.23% water, 2.56% Sultone foamer, 3.1% sodium octyl sulfate, 2.1% polyoxyethylene monooctyl phenyl ether, 0.12% sodium lauryl sulfate, and 0.05% tolyltriazole. The following summary applies to a mixture with incompletely characterized concentrations o f impurities. Data may not accurately reflect the toxicity o f the fluorochemical component o f die test sample. METHOD:_________________________________________________________ Method: OECD 201 and EPA-600/9-78-018 Type: Acute Static GLP: No Year study performed: 1991 Species: Selenastmm capricomutum Source: In-house culture. Originally from the Culture Collection of Algae at the University of Texas at Austin. Element basis: Algal cell counts (cells/ml) Exposure period: 96 hours Statistical Methods: Performed using standard statistical techniques. Stephan, 1983. NOEC was calculated using Dunnett's test. Analytical monitoring: pH and temperature Test Conditions: Algal Nutrient Medium: Nutrient medium per U.S. EPA, 1978 prepared in deionized water. This nutrient medium provided all mineral nutrients essential for algal growth and also served as the diluent for all algal operations. The pH of this synthetic algal medium was ~8.0. Stock and test solution preparation: A 1000 mg/L primary stock solution was prepared in algal medium. Aliquots were added directly to algal medium in test vessels to create test solutions. Exposure vessels: 250 ml_ Erlenmeyer flasks containing 100 mL test solution and capped with inverted glass beakers. Agitation: Continuous rotary shaker, rate of rotation not given. Number of replicates: 3 Initial cell loading: 1.0 x 104 cells/mL. 001160 Number of concentrations: five plus a blank control Lighting: Continuous lighting at 50 pEin/sec/m2 using cool-white fluorescent lamps. Water Chemistry: pH range (0 - 96 hours): 8.2 - 9.9 (control exposure) 8.3 - 8.6 (1,000 mg/L exposure) Test temperature range (0 - 96 hours): 23.0 - 23.4C (incubator) RESULTS Nominal concentrations: Blank control, 150, 250,400, 600, and 1000 mg/L Algal Growth Response at 96 Hours Nominal Avg. Cell-Count Avg. Specific Exposure (cells/mL) Growth rate % Change(1) Cone. (mg/L) Control 479,000 (M) 0.040 -- 150 188,000 0.031 22 250 58,000 0.018 55 400 20,000 0.007 82 600 3,000 -0.012 100 1000 0 0.000 100 (1) Average growlth rate in control compared to test concentration growth rate. Element values: 96-hour ErCso = 230 (212 - 248) mg/L 96-hour NOEC = <150 mg/L Element values are based on nominal concentrations. Reversibility of Growth Inhibition: Not determined. Remarks: Testing was conducted on a mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone. CONCLUSIONS__________________________________________________ The test substance exhibits a 96-hour EC50 of 230 mg/L with a 95% confidence interval of 212 to 248 mg/L. The 96-hour No Observed Effect Concentration (NOEC) was <150 mg/L. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 001161 DATA QUALITY Reliability: Klimisch ranking = 2. Testing meets the criteria for quality testing. However, sample purity was not properly characterized and the study lacks analytical confirmation of the amount of fluorochemical proportion in the solution. REFERENCES____________________________________________________ This test was conducted by EnviroSystems Division of Resource Analysts, Incorporated, of Hampton, NH at the request of the 3M Company, Lab Request number J1884-1, 1991. O T H E R ___________________________________________________________ Last changed: 6/26/00 001162 001163 iptpv;\-`i;." ' f e ' ';:- y : - M .... ' i BEST COPY AVAILABLE > Z . SOGORY. '' _ ' '.'. -AVfc-'*i V . The acute toxicity of FC-203CS to the alga, ^eleaaatriui eapricorautum, ia described in this ;final report. The test was conducted for 3N Company for 96 hours during July 8 to July 12, 1991, at the EnviroSysteas Division of Resource Analysts, Inc. in Hampton, New Hampshire. It was conducted by Jeanne Nagazu, Peter Kowalski, Ellen /Stanford, Robert Boeri, and Timothy Hard; / r U ^ :: .... V ,:.-: . * ' . The test was performed under static, conditions with five concentrations of test substance and a dilution water control at a mean /temperature of 23.2C. The dilution water was synthetic media prepared with sterile deionized water; Flasks'were incubated on a rotary shaker. ' Light was adjusted to 50 uSin/sec/a*!/! with a photoperiod of 24 hours light and 0 hours dark. Nominal concentrations of FC-203CE were: 0 mg/L (control), 150 mg/L, 250 mg/L, 400 mg/L, 600 mg/L, and 1,000 mg/L. Nominal concentrations were used for all calculations. Algae used in the test was from an in-house culture that was maintained under test conditions. Exposure of algae to the test substance ^resulted in a 72 hour ECS0 of 340 mg/L FC-203CE and a no observed effect /concentration (NOEC) of 150 ng/L. The J6 hour EC50 and NOEC are 230 mg/L "Kand less than 150 mg/L, respectively.' . f,PfV ' V - >,V m tlNix ll l&i * \S' S O*/ *5 rwis I# ! \ ir 001164 JUtS.%-mV i - -..-3-\<*u..: '*- s : ' - i . W - t`i.' >'. - ; V'- 001165 /TWZJF:':iVW:.3 " * * >  rr. i n t r o d u c t i o n This tody was sponsored by 3M Company, St. Paul, Minnesota. The objective of the study was to detersine the acute toxicity of FC-203CE to a freshwater alga. The report contains sections that describe the aethods and saterials employed in the study, and the results of the investigation. The report also contains an appendix that presents the water quality data collected during the test. V. METHODS AMD MATERIALS TEST SUBSTANCE: FC-203CE (EnviroSysteas Sample Humber 2935A) was delivered to EnviroSysteas on May 23, 1991. It was contained in a 40 oz. plastic bottle that was labelled with the following information: "J1884-1, FC-203CS". FC-203CS (an orange-brown liquid) was supplied by 3M Company, 935 Bush Avenue, St. Paul, Minnesota. Prior to use the test material was stored in the dark at room temperature. A reserve sample (approximately 1.1 grams) will be retained at EnviroSysteas for a minimum of 10 years. DILUTION HATER: Hater used for acclimation of test organisms and for all toxicity testing was sterile enriched media (U.S. EPA, 1978) with a pH of approximately 8.0. Results of chemical analysis of a representative sample 'of deionized water used to prepare the media are presented in Table 1. vi TEST ORGANISM: \ , ;; _ V.-`" . v A l g a e u s e d f o r the test was from a culture originally procured from the Culture.iCollectionof Algae at the University of Texas at Austin and delivered.^to l,Envirosystems on May 14,1991. The identity of the; culture was 'verified^using 'an appropriate taxonomic key. sThe culture" was V_` vfitransferredMto%sterile " `enriched .media identical to' media, used;for this, p'"4stestran^m^tained at test con4itions. A & l & r - ?' v >,tiae'e*" *. ' y -J tifc, H K - sfinitiw^toxiciiy^test^was/i performed during 'Jty' B^to'12, 'fi 1991.TM^^fei^miim4s^'P*^i^weii'^bf/^the^.OBCD.-:(98SlJ .`$;The`|tesY vas (s'* | liiv^conceiitratiibius of ^t',,Tv Pms^syscimc^^-'a^diltiii-.wUrfcontrol^A'ljOOq'^/L'stMk^lutin.^.'.^ ; ^is|^repVdffin|diution wateyit\Therstock solution TOS^added directly;to ' f'dilutio^'wate^contained in ^'the^test^'vssels .without'^.the||us$f [solwn^f ((control "s K 'J ' * KReKuce/ fm -.v,. 001167 . 8PT GOPY AVAILABLE Table 1. Chemical characterization of a representative sample of: ' /[, deionized eater used to prepare test media for toxicity test ^ Parameter Unit of Measurement Reporting Limit Measured Value Organochlorine pesticides ug/L '2 HD Organophosphorus ug/L 0.5 HD pesticides Polychlorinated ug/L 0.5 HD biphenyls Hote: HD - not detected at or above the reporting limit. `rir. best a v a il a b l e Algae was randomly and equally diatributed among three replicatea of each treatnent at the rate of 10,000 cella/nl. The teat was performed in 250 ml glaaa Erlenaeyer flaaka that contained 100 ml of teat aolution. The flaaka were capped with inverted glaaa beakera. Teat weaaela were randomly arranged on a rotary ahaker In an incubator during the 96 hour teat (a random numbera table waa uaed to aelect the location of each vessel). A 24 hour light and 0 hour dark photoperiod waa maintained with cool-white fluoreacent lighta that provided a light intenaity of 50 uEs-`n-*. The number of algal cella/ml in each teat veaael waa determined viaually every 24 houra by meana of direct microacopic counts with a hemacytometer. The pH (Beckman model pHI 51 meter) waa determined in each teat flaak at the beginning and end of the-test, and temperature (ASTH mercury thermometer) waa measured and recorded daily in the incubator. STATISTICAL METHODS: Results of the toxicity test were interpreted by standard statistical techniques (Stephan, 19B3). The binomial or moving average method was used by the author to calculate EC50 values using nominal concentrations of test substance. The HOEC was calculated using Dunnett's test, which includes a parametric one-way analysis of variance (AKOVA). The average specific growth rate was calculated as the natural log of the number of cells per ml at 24, 48, 72, or 96 hours minus the natural log of the number of cells per ml at 0 hours divided by the exposure period. The percent change from control is calculated by subtracting the sample average specific growth rate from >the jcontrol average specific ^growth rate, dividing that value by the average :specific growth rate in the control and multiplying that value by 100 .'" The percent change'from control, was used to compute EC50 and HOKC>alues. ; . " -/ t ..... 'fegi-- .'* ' L v J Y ' r` \ H! V'-J Ijf aw?-* ^ ; `iSif mk S/J i m ;Page 7 of 15'; 0 Vv BEST copy AVAILABLE V X. KXSQH3 Biological and water quality data generated by the acute toxicity test are presented in Table 2 and Appendix A, respectively, and the percent of control and average specific growth rate information is presented in Table 3. The 24, 48, 72, and 96 hour EC50s for algae exposed to FC-203CS are presented in Table 4. The 72 hour KC50 is 340 ug/I>, and the 72 hour HOEC is ISO ag/L FC-203CX. The 96 hour XC50 is 230 ng/L and the 96 hour HOEC is less than 150 ng/L. VlJffigJtWAjj- M` kV i . if/'' ' i 2- r Vi vi I .MSE*93 001170 T * M e 2. ; Growth iiti fro toxicity t u t Mominal Concentration (0/1) rep. Humber ot Cell per Milliliter x 10* (hour) 0 24 48 72 96 0.0 1 10 12 120 144 372 (control) 2 10 18 106 218 450 3 10 18 136 196 615 aaan 10 16 121 186 479 150 ' l 10 14 64 ~ 88 215 'S- A '. -:''*''-',' 2 3 10 10 14 10 64 70 110 70 195 155 aean 10 13 66 89 188 250 ; i 10 20 46 50 50 2 10 20 46 85 80 3 10 20 48 75 45 aean 10 20 47 70 58 . 400 i 10 14 54 25 25 _ a -- 2 10 10 40 40 10 3 10 16 46 25 25 Y aean 10 13 47 30 20 Ao0. i 10 30 64 40 10 , .2 .: 10 22 38 20 0 mean 10 . ;2v4ii*r.a',', . "- 24 '"iff. --0 2 0 ^ &:.'22i i.%?'>..' -y-> H;y: ''.k ' S tfS i *? .lias faoli,:.. P6gM: sfe-:'35 W AK-H-TiL27 fclf''-v ^ A o ' A V :A- Vi 001171 Tabi 3. Peccant change fron control end average specific growth rate fro* toxicity teat nominai Concentration of Test Substance (ng/L) Average specific growth rate 2hr 48hr 72br 96hr 0.0 (control) 150 250 00 600 1,000 0.020 0.052 0.041 0.040 0.011 0.040 0.030 0.031 0.029 0.032 0.027 0.018 0.011 0.032 0.015 0.007 0.037 0.03 0.014 -0.012 0.033 0.026 0.014 0.000 Percent change from control 24hr 48hr 72hr 96hr 0000 45 23 27 22 -45 38 34 55 45 38 63 82 -85 35 66 100 -85 50 66 100 BEST COPY AVAILABLE fi-'. ^ ` / ' Juf .f %: vlf? _ * a ' i * V -tf', ' -. 'v.'ir;Sfc';V i ; ; \ -. f-'v:. ; t - w A ^ i ^ I l f e `- ^ .>v*...... -'5-: rI jfr' l^iAl 001172 Tabi* 4 Median effective concentrations (iCSOa) from toxicity taat Exposure. period EC50 95 percent confidence limits Calculation method 24 hours > 1,000 ag/L 48 hours < 150 *g/L 72 hours 340 mg/L 96 hours 230 ng/L -- -- 247 - 438 mg/L 212 - 248 mg/L -- -- Roving Average Prohit TCI. u r a n c u OfCD. 1984. Guidelines for Tasting of Chemicals. Section 2: Effects on Biotic Systems. Method 201, Alga, Growth Inhibition Test. Adopted June 4, 1984. Stephen, C.E. 1983. Computer program for calculation of LC50 values. Personal communication. U.S. EPA. v 1978. The Saltnastrum capricorautua Prints Algal Assay Bottle Test. EPA-600/9-78-018.' July, 1978. _ BEST COPY AVAILABLE b e s t c o p y a v a il a b l e Tabla A.l. Tenperature near-red during toxicity teat Day of Exposure 0 1 2 3 4 Temperature of Incubator,C 23.2 23.0 23.-0 23.4 23.2 Vsj:` >YA l '1 n I .* v* > '?*&#" i . / - ".- ': : . t ' ," . ;'s*.j.'-'.'' v ,, - .454..,~.<" jj. / ^/'' 001176 BEST COPY AVAILABLE Tabi A.2. pH aeaaured during toxicity test Roninal concentration (g/U 0 (control) 150 250 400 600 : 1,000 Replicate 1 2 3 1 2 3 1 2 3 1 2 3 1 2 3 1 .;* v ' 2- - PH initial final 8.2 9.8 8.2 9.8 8.2 9.9 8.4 8.7 8.4 8.7 8.4 8.7 8.4 8.7 8.4 8.7 8.4 8.7 8.4 8.6 8.4 8.6 8.4 8.6 8.4 8.6 8.4 8.6 8.4 8.6 8.3 8.6 8.3 8.6