Document aBEpkQXm1JkeJKdKKkDqdz8Ly

Monsanto ........ TO : David Wood-St. Louis-B2SD October 27, 1975 LUKE HART, OCT. 22ND H. S. Bergen CONFIDENTIAL 1 C. Paton w. B. Papageorge W. Withers W. R. Richard 1. Luke confirmed that work in progress with Calgon indicates they will be able to reduce PCB level in effluent to 0.2 ppb. Further, they are working with Crawford and Russell on installation in 1976 which will control blow down and reduce water effluent volume to 1/10 present. They expect to get out flow of PCBs to about 1 ounce per day. 2. They have been contacted by David Brown of NIOSH who requested permission to carry out epidemiological study at 6. E. plant operatives. NIOSH were not aware that New York State authorities already have such a study underway and Hart has not told them. 3. Versar (Mrs. Centos) was at Hudson Falls on Nov. 22nd. 4. Luke gave me a copy of the Bill of Particulars of the New York case, (xc attached HSB/WWW/WBP). Circulation of this should be limited. Comments would be appre ciated. On Nov. 4th, New York agency have to put their witnesses up. Barly December, G. E. have to put forward their witnesses. Luke was not aware of further input required immediately from Monsanto. They still need the promised data on a) Theoretical "half life" of Aroclor 1016 ' b) Base toxicological data; LD50 etc. of 1016. I told Luke I expected a) to be ready b) available week 27 October. d~November, and /deb Enclosure in i* ev. /* od MONS 085933 STATE OF NEW YORK DEPARTMENT OF ENVIRONMENTAL CONSERVATION In the Matter of Alleged Violations of Soction 17-0501, 17-0511 and 11-0503 of Che Environmental Conservation Law of the State of New York by: CENERAL ELECTRIC COMPANY Respondent 4 BILL OF PARTICULARS File No. 2833 4 Sir: PLEASE TAKE NOTICE that the New York State Department of Environmental Conservation ("DEC") as and for its Bill of Particulars herein, alleges: 1. Upon information and belief, Respondent used Polychlorinated Biphenyl compounds ("PCB's") known by tradename as Aroclor 1254, 1248, 1242 and 1016; the actual PCB's used by Respondent and the quantities thereof are solely within the knowledge of Respondent. . 2. Upon information and belief, the exact location of each' point source discharge is indicated in the June, 1975 report prepared for Respondent by Clark, Diets and Associates, (the "Clark, Dietz Report"), Figure Nos. 1 and 3, and shown as discharges 001-009. The exact dates, specific types and quantities of PCB discharges are solely within the knowledge of Respondent except as- shown on Exhibit "A" attached hereto and incorporated herein by reference and in the Clark, Dietz Report, Tubles 8,9,12 and 13 and Figures 10 and 13. Upon information and belief the specific typos, quantities and dates of discharge used by Respondent to derive the data or estimate, contained in Respondent's U.S. Army Corps of Engineers Permit Application No. 250-OKW-2-000677 dated December 18, 1972, wherein Respondent indicates an average discharge of thirty pounds per day total PCB's from both of MUNS 085934 ' I< Respondent1 s luanuEacturing facilities in Hudson falls and Fort Edward, New York combined and a maximum of 47.6 pounds per day from such facilities, is solely within the knowledge of Respondent. 3. The exact location of each point source is as identified in Para graph "2" hereof; the types and quantities of PCB's discharged are given in Paragraph "2" hereof; upon information and belief the entire premises owned, operated and maintained by Respondent in Hudson Falls and Fort Edward, New York (the "Premises") are a non-point source of PCB discharge; upon information and belief PCB's have concentrated in varying amounts throughout the Premises, and are known to be concentrated ty specific type and amount at the points indicated as sampling stations identified in Exhibit "B" attached hereto and incor porated herein by reference,in the amounts and types indicated therein; upon information and belief, the period of time during which PCB's accumulated at the site and the aforementioned sampling stations are only known to Respondent; upon information and belief residual and/or accumulated PCB's from non-process and non-point sources sre discharged in greater amounts during periods of wet weather as 'shown by date, amount and type of PCB in Exhibit "A"; upon information and belief the extent to which PCB discharges increase during other periods of wet weather and the amounts and types thereof is within the sole knowledge of Respondent. 4. See responses contained in Paragraphs "2" and "3" hereof ; upon in- formation and belief the location of the only other known sources of PCB's in the Hudson River are: ' NAME SAMPLED AMOUNT TYPE Hudson Falls Sewage Treatment Plant 9/11/75 120ppb 1016 Hudson Falls Sewage Treatment Plant . ' 9/11/75 46ppb 1242 Sprnguc Electric Corp. North Adams, Mass. . 9/30/75 57ppb 1016 -2- MQNS 085935 < f. / Upon information ant) belief, however, the'discharges from the tfudson'Falls Sewage Treatment Plant originated from Respondent's premises in Hudson Falls, Now York. 5. See Paragraphs "2" and "3" hereof; See Exhibits "C", "D", "E", and "F" attached hereto and incorporated herein by reference for specific locations of waters, sediments and fish contaminated by PCB's, the type and quantity thereof; Upon information and belief, virtually the entire Hudson River, downstream of the Premises are, in water, sediments, fish and other aquatic organisms cen to taminsted by PCB's/varying degrees. 6. See Exhibit "G" attached hereto and incorporated herein by reference; which, upon information and belief, shows the specific quantities of PCB's used by Respondent and all others within New York State in 1974; upon information and belief, data regarding Respondent's, and all others, purchases of PCB is Bolely within the knowledge of Respondent, such others and the suppliers and/or manufacturers of PCB's. 7. Upon information and belief, the fish, birds, wild fowl, marine and terrestrial mammals and other wildlife, the specific types and quantities of PCB, the effects and period of assimilation causing such effects are all in dicated in the references listed on Exhibit "H", attached hereto. 8. Upon information and belief, the specific types of PCB's include those homologs and isomers containing 1 to 10 chlorine atoms, represented by the mixtures Aroclor 1221, 1232, 1016, 1242, 1248, 1254, and 1260; the exact 'chemical composition of each type of PCB's is within the sole knowledge of Respondent and/or Monsanto Chemical Corp. A detailed description of the PCB's is presented in The Chemistry of TCP's by Hutzinger, Safe and Zitko (CRC Tress, 1974, pp 7-40). HONS 085936 -3- .t - ., !,, 1. Upon information and belief, the water solubility of some PCS mixtures ere summarized below: TYPE OF PCS Aroclor 1242 Aroelar 1248 Aroclor 1254 Aroclor 1260 ESTIMATED WATER SOLUBILITY mg/1 0.24 0.054 0.012 0.0027 Upon information and belief, because of the chemical similarity of Aroclor 1242 and Aroclor 1016, the solubility will be approximately 0.24 mg/1. Upon information and belief, the bioconcentration of PCB's in, for example, oysters where the bioconcentration factor expressed as ratio of the concentration in oyster to the concentration in water, is as follows: PCB CHLORIDE CONTENT 2 cl 3 cl 4 cl (sym) 4 cl (asym) 5 el 6 cl BIOCONCENTRATION FACTOR 1,000 7,500 8,000 12,000 28,000 48,000 Upon information and belief the bioconcentration factors for Aroclor 1242, Aroclor 1248, Aroclor 1254 and Aroclor 1260, for example, in minnows range between approximately 5 x 10^ and 25 x 10^; because of the similarity of Aroclor 1016 and Aroclor 1242, it is believed that the bioconcentration factor for Aroclor 1016 in, for example, minnows is approximately 5 x 10^. HONS 085937 -4- ' Ujifcsn information afid belief, PCB's are much more soluble in'oils chan in water, e.g., PCB's (2,5,2' - trichlorobiphenyl; 2,5,2',5' - tetrachloro- biphenyl; and 2,4,5,2',5' - pentachlorobiphenyl) are 10,000 times more soluble in n-octnnol than in water. . Upon information and belief, PCB's are highly non-biodegradable, e.g., the percent degradation of PCB mixtures in a 48 hour activated sludge treatment cycle is as follows: TYPE OF PCB 2 DEGRADATION (48 hours! Aroclor 1221 81+6 Aroclor 1016 33 + 14 Aroclor 1242 26 + 16 Aroclor 1254 15 + 38 Upon information and belief, with shorter periods of treatment, Aroclor 1254 was not degraded in 5 - 10 hours, but the PCB's were dissolved in the fats present in the sludge and were absorbed at the surface of suspended material in sludge. Upon Information and belief, because microbial activity is more intense in activated sludge systems than in most natural waters, PCB mixtures are persistent in natural waters. ' 9. Upon information and belief, PCB's discharged into the aqueous environment become distributed in sediments and are available to bottom dwelling organisms for uptake into the body, See references contained in Exhibit "H with respect to solubility see Paragraph "8" hereof. 10. Attached hereto as Exhibit "E" and incorporated herein by reference arc the specific locations of each Hudson River sediment sample completed to date, the specific types and concentrations of PCB found therein. . HONS 065938 1)!. Attached hereto as'.Exhibits "C",."D">. "E", an'd "F" an^ incorporated herein by reference is a tabular description and quantification of specific instances of Hudson River despoliation and degradation, including its waters, sediments and fish. Upon information and belief, the degradation and despoliation have occurred over at least the past twenty years. 12. See Paragraphs "2" and "3" for a description of each discharge, the date, location, type and quantity of PCB's. Upon information and belief, knowledge as to any other discharges, the dates, types and quantity thereof are solely within the knowledge of Respondent. Upon information and belief, these discharges represent a hazard to human health because the PCB's so discharged are bioconcentrated in aquatic organisms, including edible fish, to levels above five parts per million (ppm). The United States Food and Drug Administration ("FDA") has established 5 ppm PCB's as the level above which fish should not be eaten because of the toxic effects of PCB's (boc government's Exhibit 3 for identification). Upon information and belief, research studios have shown the toxic effects of PCB's in subhuman primates (Exhibit "H"). . Upon information and belief, due to the non-blodegradable characteristics of PCB, the environmental contamination shown by Exhibits "C", "D", "E", and "F", attached hereto, the discharge of PCB's has and continues to cause irreparable damages to the Hudson River water and aquatic organisms. Upon information and belief, this environmental contamination exists continuously to varying degrees throughout the Hudson River (below Respondent's manufacturing facilities) and the coastal waters of the state. Upon Information and belief, because the aforementioned discharges cause and/or contribute to the concentration of PCB's in edible fish, such discharges MONS 083939 render .'the commercial fishing resources of,,the Hudson River untjqrket^ble. The name, address, location and description of each commercial and recreational interest is unknown to the Deportment. 13. FDA has promulgated rules and regulations limiting the concentrations of PCB's (of any type) in certain foodstuffs, including fish and certain fish products; the United States Environmental Protection Agency has proposed water quality criteria which would establish 2 parts per trillion of all types of PCB's in waters as a Water Quality criteria under the Federal Water Pollution Control Act Amendments of 1972 (33 U.S. Code 1151 et seq.); the Advisory Board of the Inter national Joint Commission on the Great lakes has recommended a concentration for all PCB's in fish in the Great Lakes of no greater than 0.1 ppm. 14. Upon information and belief, such information is solely within the knowledge of Monsanto Industrial Chemicals Corporation, ("Monsanto") Respondent and others purchasing PCB's from Monsanto. 15. See government Exhibit 3 for identification for the exact text',' time, date of the FDA determinations. Upon information and belief, the FDA has sole knowlsdge of the other information requested. 16. See Exhibit "F", attached hereto. 17. See Paragraphs "2" and "3" hereof. 18. See Exhibit "I" attached hereto and incorporated herein by reference. 19. Upon information and belief, the discharge of any type of PCB's in amounts, together with background, which will result in excessive levels of.any type of PCB in fish will impair the receiving waters for their best usage, i.e., fishing. MOWS 085940 20\ See Paragraphs "2" and ''3" hereof; Upon Information anil belief, these discharges, together with background, cause the receiving waters and the waters into which the receiving waters flow to be Impaired for their best usage. 21. See Paragraphs "2" and "3" hereof; Upon information and belief, the dis charge of PCB'e-by Respondent, together with background, has and continues to be in quantities injurious to fish life and protected wildlife and waterfowl and/or the propagation thereof. Dated: October 14, 1975 Yours very truly. Net; York State Department of Environmental Conservation 50 Wolf Road Albany, New York 12233 Philip H. Gitlen Assistant Counsel (518) 457-6695 HONS 085941 -I. STATE Or NEW YORK COUNTY OF ALBANY ) ) ss.: ) Eugene Seebald, being duly sworn, deposes and says: That ho is Director of the New York State Department of Environmental Conservation, Division of Pure Waters, and is acquainted with the facts and circumstances of the above entitled action; that he has read the foregoing Bill of Particulars and knows the contents thereof; that the same is true to his knowledge except os to the matters therein stated to be alleged upon information and belief, and that as to those natters he believes to ba true. Swqrn to before me this MONS 085942 General Electric Company Hudson Falls (T), Fort Edward (T), Washington County On-Site Effluent Monitoring Program for PCB's Dates: August 19-26, 1975 Time Span: All samples composited over the full length of the operating day.at both plants. ' Personnel Involved: Representatives of Region IX-EPA and New York State DEC - Division of Pure Waters Location: See attached drawings Samples Analyzed By: EPA laboratories - Edison, New Jersey A. Summary (lb/day as Aroclor 1016) Fort Edward Plant 8/19 Gross Discharge Intake Level Net Discharge 0.11 0.40 -0.29 8/20 0.90 0.18 0.72 8/21 1.14 0.19 0.95 8/22 1.32 0.25 1.07 8/23 (1) 1.04 0.10 0.94 8/24 (1) 0.24 0.21 0.03 8/25 (2) 5.51 0.23 5.28 8/26 (2) 4.79 0.17 4.62 Hudson Falls Plant (1) (2) (2) Gross Discharge ` 0.50 0.19 0.10 0.26 0.16 0.09 0.19 0.40 Intake Level Net Discharge 3.40 0.47 0.87 0.60 0.36 0.79 0.30 0.79 -2.90 -0.28 -0.77 -0.34 -0.20 -0.70 -0.11 -0.39 Total Gross Total Net 0.61 1.09 1.24 1.58 1.20 0.33 5.70 5.19 -3.19 0.44 0.18 0.73 0.74 -0.67 5.17 4.23 Average Gross: 2.12 lb/day Average Net : 0.96 lb/day Notes: (1) - Plant not in operation (2) - Rainy weather EXHIBIT "A" MQNS 085943 B. Complete Sampling DaCa All concentrations are expressed in micrograms/liter as Aroclor 1016. All flow are expressed in MGD. Tort Edward ]Plant 8/19 8/20 8/21 1. Intake Well (cone.) 60 28 :18.8 (flow) 0.79 0.79 0.79 (lbs) . 0.4 .,0.18 0.19- 8/22 38 0.79 0.25 8/23 42 0.29 0.10 8/24 84 0.30 0.21 8/25 35 0.78 0.23 8/26 25 0.80 0.17 2. 004 (cone.) (flow) (lbs) 16 0.79 0.11 137 0.79 0.90 173 0.79 1.14 201 0.79 1.32 430 0.29 1.04 95 0.30 0.24 847 0.78 5.51 719 0.80 4.79 Hudson Falls Plant 3. Intake (cone.) (flow) (lbs) 340 1.20 3.40 52 1.08 0.47 88 1.18 0.87 54 1.33 0.60 35 1.24 0.36 76 1.24 0.79 27 1.33 0.30 67 1.41 0.79 4. 002 (cone.) (flow) (lbs) 34 1.03 0.29 24 0.88 0.18 :10.4 0.98 0.085 27 1.12 0.25 17 1.07 0.15 9.4 1.07 0.084 20 1.10 0.18 39 1.17 0.38 5. 006 (cone.) (flow) (lbs) 6.8 0.072 0.004 3.3 4.5 (all days) 0.002 0.003 4.1 0.002 3.1 0.002 2.8 0.002 3.7 0.002 4.3 0.003 6. 008 - No PCB's detected. Sampling stopped after 8/21. 7. 003A (cone.)_ 255 (flow) (lbs) (1) 0.21 8.6 (1) 0.007 10 (1) 0.008 11.6 0.11 0.011 1.8 0.07 0.004 2.5 3.9 0.07 0.129 0.001 0.004 10.8 0.129 0.012 S. 003B (cone.) (flow) (lbs) 3.2 0.03 2.4 0.03 2.8 5.0 0.03 0.03 0.0008 0.0006 0.0007 0.001 5.5 0.03 0.001 1.1 9.5 4.5 0.03 0.03 0.042 0.0003 0.0024 0.002 Notes (1) - Not available MQNS 085944 General Electric Company Hudson Falls (T), Fort Edward (T), Washington County On-Site Soil and Storm Runoff Sampling for PCB's Date: September 25, 1975 Fime Span: 3:30 - 6:10 P.M. Personnel Involved; L.J. Nadler, T. Chung - NYSDEC " Division of Puie Waters (1) Locations: See attached drawings and description Samples Analyzed By: N'.Y.S. Dept of Health at Griffin Laboratories Soil Samples Sample Number Plant Specific Aroclors 1016 1254 (micrograms/gram) 1221 11 PCB 702 11 PCS 703 11 PCB 704 11 PCB 705 11 PCB 706 11 PCB 707 11 PCB 708 11 PCB 709 11 PCB 711 11 PCB 712 F.E. F.E. F.E. F.E. H.F. H.F. H.F. H.F. H.F. F.E. # 57 2800 1100 900 3400 24,6 90 > 100 ^ 87 760 14 0 630 173 259 217 0 Water Samples (grab) Sample Number Plant Time Specific Aroclors 1016 1254 (micrograms/gram) 1221 11 PCB 701 11 PCB 710^ F.E. H.F. 3:30 P.M. 5:45 P.M. Notes (1) - Mr. R. Marquis and Dr. R. Stengler of Ceneral Electric accompanied and received a duplicate sample at each location. (2) - Instantaneous flow measured at 0.93 gal. /min., using watch and bucket. ' MOWS 085945 .. i '* ' rA is*!-* (1) J1-7C.3-711: rhift in a y'r P vater sample ;Ja'cc frail a par^iny lot drein 0/i tna plant pt`?rt'j After xo-avica die cretin?. Semple collation started Jf-prorioacely JiJl pa. fU Jl-PC.s-701* rsic in a seii saoplo cakes adjacent to the leading dock. - (1) Ji-JC*-7ai rhir io a soil saopie tala in t& leach field ran at a point pp/esiajceJp 75 fne iroj tin eoctheose corner of .the /oner indicated on tin attached dracinfa, ana ou a direct lino triu she eastern eld 1 ti fence. (4i t this io soil saopio tale dlrjcciy tonoceh cite Pyreeol unloading area. ` (5> ii-rci-705/ ?M is 4 seii soapla takon tlireotlp adjacent to tin soiid vest contactor located ocac to tho Parasol unloading area. (if ll-PCO-712* tfiio is os offsitm soil eaeple toko on tie east si do f Allen Street duo vejt.of tho plant, and directly across tho stress froa 4 disysra .'loAoioA sub-statioa. 07CSQ;/ pax>&5 Pi,iKr (7; ii-Pcs-7c/<; / This is a soil *<tpJ eekea sc s spot located A&out 50 /t froa rscjr door of puiidinv it, cad on the center Use of t.lo pate. ' (Of ii-tco-rot v*ti is soil staple taken eJJaoeot &o~to solid vac tv eoepsetor Jocatod set of btildinj ili Appronijeeteiy 4 ir.c.zos of gravel vure alcsroi oucf to expos soil. (Of Jl-Tcs-7ti61 mis is o soil sample taken late t:/o tree* sidings adjacent to th Pyrsfloi onloading ores. ` . (13) .ll-kC'J-70), Titio in & toil stnpXo taken in tho southwest corner of eft took fora adjacent .to a pisefern on th north side of iaiitrisff dl. approximately 5 inches of envoi veto cleared away to expose coil. , flip 2J-PC6~71j; rhi is $rr.-i> eater ssapJo fro dlaeUappc 007. Saapics voro oolioeted In plastic I/tcAui levered ftp rope, hoglor.inc. At cpproulcatalv 3it$>p. Tic cjsi.>lee appeared turolJ rod contained considerable asdinent. After collection of the rcnpla, s crudg ceeeere.ssnt of the Plow r.-os a,<o by Hearing tho fell diachsrjo (row tio pipe for period of 70 seconds. Tho volcita collected during this purled vj; raSixsetvtf ck 1.75 .liters, usln-f the scale on the i:mi! surface of eiio bucket. Thin dieeJv.nr&e ie xopexcot: to rr;:tnin onlj etoro wfiwr. fi;o nuuplv sallvcc.ti e.% c/ia second of ttto ccaeoctitiw deyr of rainy cojfcecs*. MQNS 085946 (Uf 22-PCn*7J.1; mis 1c e sol: ear-pie eeil<rcind Jost ostsiuo peskiiu! lot *C- of fendy ,i?Ji CofferJt Jen, ni'e.it .to-cunreor south >{ t*i.> pl:.nr. cs iile.r 'JztCut. AVMarono HUNS 0 8 5 9 4 8 i n; 'GUM i _5ITS pLAii ityotCH Mti* nAnr I! INSTIGATION 0? POLYCHLORINATED BIPHZNTLS I>< THE HUDSON RIVER <KGDS3X FALLS - FT. EDWARD AREA). Report Preoared 3v; Rovai J. Nadeau and Robert ?. Davis Report Approved i>v Royal'* JV'Nad'eau, Ph.D. Chief, Biology Section x; rSncLs T. i-.rezens'ci Chief, Technical Support Branch Surveillance & Analysis Division EXHIBIT "C HONS 085949 Introduction................... facariaia .and Me choc a 1 Descriotion of ^amt>lin>? Area - Hudson 'diver........................................... Chemical Analysis - Water................................................................................... Chemical Analysis - Sediment..................................................................... Chemical Analysis - 3i.ota..................................................................................... Histology................................................................ .. ........................................................ A 6 6 6 7 Results Field Observations.......................................................................... Chemical Analysis - 'water and Sediments................ 3iological Tissue................ Kiscooathology................. ....................................................... *................................. 7 g 10 11 Discussion.................................................................................................................................... 12 Conclusions................................................................................................ 13 Recommendations....................................................:.................................... .................. ........15 103 L*S Table 3 - Biota Collected During the Hudson River Investigation.............................. 16 Table 4 - Analysis of Water and Sediment Samples of Aroclor 1016 from the Hudson River in the Area of Fort Edward, Mew York.................. 17 Table 5 - PCB Concentrations in Snails and Fish Above Baker's Falls (Control Area) on the Hudson River.......................... 18 Table 6 - PC3 Concentrations in Snails and Fish Below the General Electric Discharges on the Hudson River.......... 18 Table 7 - Concentration Factors of PCB's in the Hudson River.. 19 literature Cited.................................................................................................................... 20 Figures Figures I through 23 *.v* b* MONS 005950 L ...... --...... I yoychior i:v.i'..u-J l j (PJ'J' oil/ ?s rac .'n : Ly a; i/io, have bi-: -'sliced .ns an nvvrormen ta i c.aalur.u.un,:. Prirarily ujsd in Industry, PC3 .*r2 even ~Q?2 persistent than. DDT. Tn*se compounds *C2 sssanCiilly r.on- nitcrable by microbial or physical-chemical activities and <ir; Incor^orabl into living protoplasm. The molecular structure given h^iov inhibits enr/ presently found Ln r.acurai cellular v.-srams, iron shearing Che bonding between che chlorine aCom and Che biphenyl structure. Paradoxically, this same structure gives specific chemical characteristics very desirable for certain industrial uses, i.e. manufacturing of plastic resins and varnishes. ?C3`s not only are incorporable into living biomass in natural ecosystems but are transferable within food webs; Che end result being a much higher . concentration of these compounds occurring in specific tissues of summit carnivores (biomagnification). Occurrence of PCB's in human tissues has been documented especially in people who are constantly exposed to PCo's through their occupation or life styles. Even though these compounds have a com paratively low acute toxicity for mammals, the long term effects may be i; A- A much more insiduous and devastating. A number of investigators have shown that PCS's induce production of liver microsomal enzymes. Others have shown that PC3's can decrease Vitamin A content in the liver (Cecil, et_ al, 1973>. Trsnsolacental passage of PCS'3 has also been shown (Crane, t al_, 1971). t t PC31 s have been detected in human adipose tissue In such widespread occurrence that 41-45'A of the U.S. population contains 1 ppm or more (Price & Welch, 1972) MONS 085951 1 mia the nation's fresh me ca-astal vv.ars (:ifso*t jnd Surofin. 1972). .-.i indication of the videsoraac! contamination of ?C3'.5 in coiiav's sociecv Li illustrated in Che Temporary Tolerance Limits sec by Che FDA in certain food produces (Ximcbcough 1974). TABLE 1. Temporary Tolerance Limits of pen's Substrate Milk (fat basis) Dairy Products (fat basis) Poultry (fat basis) Eggs Complete and finished animal feeds Animal feed comoonents Fish and shellfish (edible portion) Paper food-packaging material Level 2.5 2.5 5.0 .5 .2 2.0 5.0 10.0 The water Quality Criteria of 1972 <Z?A, R3-73-033, March 1973) does make recomendations on the basis of body accumulation of PCB's. Estimates indicate that 41 to 45 percent of the general population in the U.S. nay have levels of 1.0 mg/kg or higher (wet weight) in adipose tissue. It therefore was calculated that a daily intake of*0.02 mg would require about 70 years to be toxic. Utilizing a safety factor of ten would perait a daily intake of two micrograras. In general, the average person drinks two liters of water per day. With this intake a permissible concentration in water would be one ug/l. . PCB's in Region II The General Electric facilities at Hudson Palls and Ft. Edvard, New York report PCB's in their effluehcs (TABLE 2). * Does not apply to packaging material that is separated from the food by a barrier impermeable to. PCB migration. ^ 2 MONS 08595^ 2. .`4 5. urv- i l; Jen* *- :: * (Jourcj; *: it .\op l icu ri jns ) Cumber Cu trail Location Daiiy Aver Conc3n:rat ion 1) Oil/Crease PCS Average Doilv Loadin'; (lbs.5 Oil/Crease PC3 Itaximun Daily Load ir*2 > lb s . ' 00! Hudson rails 13.7 .5 239.3 10.0 250.9 17.i 003 Hudson Falls 2.1 -- 4.9 ------- 5.25 ------- 004 Ft. Edward S.9 5.0 33.27 20.0 44.5 30.0 fr Oil and grease is listed being that PCS 's are e:c Crenel*/ soluble in the oil ar.d grease component of a waste stream; oil and grease serves as a carrier of ?C3's into the receiving waters. -- a.-; \. PC3's are used in large quantities for filling capacitors. and transform ers manufactured and assembled at both the Hudson Falls a nd Ft, Edward facilities. Both plants have waste treatment processes for removal of chemicals, however, significant PC3 and oil and grease levels still remain in the waste stream (TABLE 2). To date no water quality standards have been promulgated specifically for ?C3 compounds, making it difficult to initiate an enforcement action on the basis of the loading and effluent discharge concentration alone. The approach being used by the Region II Enforcement Branch (see attached memo: Sandra Kunsburg) is, that the discharge from the General Electric facility may be presenting a health hazard within the mean ing of Section. 504 of the.FWPCA. Within this Section, a suit can be brought against the discharger if it can be shown that the discharger is presenting an imminent and substantial endar.germent of the health of persona through the ingestion of wat%T or food contaminated from the discharge. The Biology Section was requested to submit a plan of action and scopeof-work for performing an investigation, in the vicinity of the General Electric facilities, to determine the presence and extent of contamination of water, sediments and biota of the receiving waters, the Hudson River. -- !. * v. U t. 3 MONS 085933 'of jamoii:::; Area - '.vIjop, Il'/sr Szzzzon 0 jcacion 0 is located .3 miles above Baker FaLLs in th 2 impoundment acsa (FIGURES 1 & 2). This station is upstream and separated from both General Electric facility outfalls by a hydroelectric dam (height - 15 n>. At sampling time (3:15 pa, August 12, 1974) the water was heavily laden with fibrous particulates being carried down from an upstream source. Several . paper mills and fiber board manufacturers are located in Glens Falls, 3-5 miles upstream from Station 0. In the main channel area the bottom is hard shale. In the shallows where water velocity is less, large deposits or . . sediments intermixed with bark, branches, lumber slabs and cinders are * found. A sediment sample was taken by coring into the sediments with a glass sampling jar and capping it underwater. A subsurface water sample was taken nearby. A seine was used to collect fish near a patch of emergent vegetation on an opposite bank from the sediment deposits (east bank). The gastropod (snail) populations associated with the emergent vegetation were sampled simultaneously Station 1 Station 1 is located at the junction of the outfall stream from the Ft. Edvard facility and the Hudson River (FIGURE 3). At this point, the Hudson River is a roaring, turraoilous river with a high velocity, high volume streamflow (4466 cfs)*. The"river is heavily laden with heavy suspended loads of fibrous material. Water and sediment samples were taken at this . point in the same manner as at Station 0 (9:13 am, August 13, 1974). The * sediment sample was taken from a small submerged cinder-gravel spit right at vf- ^Calculated from M.Y. State Water Resources Data Book 1971. HONS 085954 4 ill ' iric n .\t\ c t'ioomI.k c. j?, ..Ti i-d I'iu sn't^T'** livers obov* Che junction. Fhid station is only act ussible by ?oac in. chjt the river banks are precipitous shale rock faces, 13 m in height. This is Che only accessible outfall being chac the upstream Hudson Falls facilities cannot be reached by toot or boat. Station 2 .. Station Z is located about 0.25 miles downstream from the outfall junction (FIGURE 4) where water and sediment samples were collected (10:00 am, August 13, 1974). River flow conditions are the same as at Station 1. }!o macroinverte brate or piscine populations were observed at this station. The water is laden with the same fibrous suspended materials as evidenced at Stacion 0. The * river bottom is mainly a shale ledge. A sediment sample was collected from a remnant shale flake, cinder deposit bank above the high water mark. All the collecting sites downstream from Baker- Falls were submerged until September 1973 when an abandoned hydroelectric dam at Ft. Edward was removed. The water level was drastically lowered, 5-15 meters. Station 3 Station 3 is located about 0.5 miles downstream from Station 1, water and sediment samples were collected (11:00 am, August 13, 1974). In this area an extensive sludge bank exists as a remnant of an industrial era gone by. The bank sediments consist of gravel, cinders, lumber slabs and bark from logging activities, forty years ceased (FIGURE 5). The sediment sample was collected from a submerged deposit near the same area where several fish were collected by seining (FIGURE 6). In the shallow area, the fibrous material, suspended at the upstream stations has flocculated onto the river bottom, coating the bottom with a paper wrapping (FIGURE 7). 5 MGNS 085955 S;:aci.cn 4 ,'itacion 4 is located 0.75 miles downstream from Station i. T.ie river is slightly wider ac this point with decreased velocity. Fish populations arc abundant,in the shallow shoreline areas. These populations were samoled by seining (FIGURE 3). Water and sediment samples were collected in the river, three (3) meters upstream from the junction of a small stream that flows in on the west shore (1:15 pm, August 13, 1974) (FIGURE 9). Chemical Analysis - Water* A measured volume of sample was extracted with hexane. The extract was dehydrated, concentrated and analyzed by computerized gas chromatography/ mass spectrometry (GC/MS). Sediment** The sediment sample was partially- dried and extracted by column elution, vita a mixture of 1:1 acetone/hexane. The extract was washed with water to remove the acetone and then the polychlorinated biphenyls were extracted from the water with 157# CHgClg in hexane. This extract was then dehyrated, concentrated to a suitable volume and analyzed by computerized GC/MS. Biota (See attached memos:. Gilman D. Veith, Ph.D.) The samples were blended with anhydrous sodium sulfate (prewashed with redistilled acetone) and the homogenate was extracted with a mixture of hexane and ethyl ether (3:1 v/v). The extracts were placed on a 20 gm *'fMethods for Organic Pesticides in Water and Wastewater 1971. Environmental Protection Agency, National Environmental Research Center, Cincinnati, Ohio **Analysis or Pesticide Residues in Human and Environmental Samples 1951. Parrinc Primate Research Laboratories. Environmental Protection Agency, Perrine, Florida, Section llB:l-6. 6 MGNS 065956 i '.or'.s mi;'.', .IJ -,[ vns ad;u,ccu so the cinaL concentratin': of LvJV > -.'h., \slz:\L* -:`.m linear .mg* or the 52s chromacograpii. The sample of rod; b?.ua ream -he discharge required a 50 fold dilution before analysis. . The presence of PC3's in all samples was confirmed by prechlorinatirtg Cha samples with SbCl^ for four hours sc 17CC to torn Oi^OI^q. The CmC'iq was subsequently analysed by gas chromatography using a three root column or 37, Dexsil-300 at 200C. Subsequently, a CC/MS analysis of the rock bass extract was- performed along with analysis on Aroclor 1016 and 1242 standards. - I; r Histology ' Four (4) specimens were collected from Station Q (control) 3nd Station 4 1 and preserved for histological examination. These specimens were representative of the Northern Common Shiner, Notroots comucus frontalis populations found a: each station. The fins and tail were excised and the whole specimen embedded in paraffin in preparation for sectioning. After sectioning, selected sections were mounted and stained*. t,?v RESULTS Field Observations .. . ' A specular observation at Station l is the oil on water plume resulting from the confluence of the waste stream and the Hudson River (FIGURES 10 & 11). The plune is visible 20-30 meters before being completely emersed and broken by the turbulent river waters. At the same time a pungent kerosine odor is ^Staining method supplied by Mr. Rudd Douglas, Department of Zoology, Rutgers University, Mew Brunswick, New Jersey. 7 HONS 08595? r-:V>\a river oar.i (?I0U7. 121 U 3? Us-. ;red eai .'-sne-.rjrj-* j;- oil 2r.d jrs.i.ie r:on the effluent. . Gastropods wers collected foe analysis above the outfall river con fluence; however, downstream no visible signs cf attached or ocher toms of aquatic life vers evident as far as one can easily negotiate walking along the steep rivar bank (approx. 200 meters). At Stacion 2, a similar lack of attached forms was evident. At Station 3 several small fish were collected in the shallows (TABLE 3). At Station 4 the bank consisted of shale ledges forming several shallow shoal areas running diagonally into the mainstream. Sediments are deposited in the shallows. The organics brought downstream sustain a diverse gastropod population, represented by Phvsa. Heliosoma, and Linnacea. A seine haul in these shallows produced an abundance of common shiners, Notroois cornutus - frontalis (Agassiz). ' The ecology of the Hudson throughout the sampling area (Station 0 being the exception) has been drastically altered by the destruction, of the iopound- ment once formed by the Niagara Mohawk hydroelectric dam at Ft. 'Edward. Once the ecology resembled that typical of any impoundment, pond or lake; submergent- energent vegetation communities along the edge and deep water communities in the middle. In its place now is a rushing, roaring river that has cut new channels through the bottom deposits, exposing once buried trees, logs, several model T's, baby carriages and boulders (FIGURES 13 & 14). Heedless to say, the biomass and community structure is rather sparse and deprived. Chemical Analysis Water & Sediments Qualitative detection of PC3's in water and sediments was accomplished ' 8 MONS 085958 : .`roari r. { - jr.out jr m /in ii bo-c `IM ot vi: \:zziy: Ti:::urjs inducing 1016 >u?p Li..*:! by General Electric and Research Zrian^i- ?3-;.v -j-.-h atv/ironnencai sa^pii extracts. A gas chromasograra (PIGURS 15) revealed th* high concentration of trichloro biphenyl compounds characceriscic of AcocIjc 1015 mixture. The mass spectra of Arocior 1016 compounds were then compared with those extracted from the water and sediments. . ?C3fs identified as Arocior 1016 were found in the water samples at detectable concentrations at all sampling locations except Station 0 (control) and Station 4 (furthest downstream) (TABLE 4). The highest concentration of Arocior 1016 in water was 2300 ug/1 at the outfall site. The downstream stations, (2-4), however, demonstrate low levels of PCB's. These low levels are largely a result of effluent concen trations being dispersed by river water. At all stations the sediments contained higher concentrations of Arcelor 1016 Chan the water column resulting from adsorption of PCB's on suspended or already settled materials. At Station 2 the contamination must have occurred prior to the removal of the hydroelectric dam. The sample site has not been inundated for at least, a year. The high contamination level at Station 1 indicates the adsorptive capacity and constant exposure of the sediments nearest the outfall to the Arocior 1016. These sediments were also heavily laden with noticeable oil and grease which serves as a solvent for PC3 compounds. At Station 3, high concentrations (2980 mg/kg) occur in the sediments, representing accumulated levels of PCS's, 10^ fold greater than the outfall concentration. The high level is a product of historical plus present ad sorption and deposition of PCS compounds. The PCS level at Station 2 i* * U` 9 MONS 085959 ; r*~ ir-5uori:2i imposition "..ii.n.1 . 1 : ,;o-::nr,Ta l i- lis<;ecC3o -c Lime 3 at 1, > cc^ur ia ; Liaificanc iu arK iti 2s in ch s ^irrents. 'these conccacracions raust be considered bacic- ground levels in that similar levels occur at Che control area as veil. 3iologicsl Tissue . Station 0 the samples collected above the discharge contained the later-eluting pea's with retention times of 84, 98, 104, 112, 125, 146, and 174 relative to pp DDE. These are characteristic of Aroclor 1254. In addition, major PCB components were found at relative retention times of 37, 40, 47, 54, 53, 70, and 78. FIGURE 16 presents a chromatogram of a control station sample and the major components are enumerated by relative retention time. The relative concentration of the PC3 components together with the absence of ?C3 components eluting earlier than 23 suggest that the PCB's in these .`...f samples can best be estimated as a mixture of Aroclor 1254 and Aroclor 1248. Using the later-eluting components with Aroclor 1254 and the earlier major components with Aroclor 1248, the PCB concentrations in the control samples were estimated and are presented in TABLE 5.* Distinctly different from the samples from the control area were the samples collected in the vicinity of or below the General Electric discharge. FIGURE 17 presents a chromatogram of the sample of bass below the discharge and it is evident there are no major PCB components eluting after 78 relative to DDE. Moreover, the 21, 28, and 32 components are greatly enriched relative to the 37 component. This suggests that Aroclor 1242, Aroclor 1015, or a mixture of these two formulations are present in the Hudson s\Y 10 MUNS 085960 turranrs oi the kudson Riv*r mc}: ;? extract with rho** .>r VrocL.'r *i"i' and Aroclor 1016. The nass chrocutcagrana are similar virh che ex ception oc the peak eluding at spectrum ISO. FIGURE 19 shows the mass chromatograms for the characteristic ions of triehlor PC3 as can be seen by the comparison of the mass chromatograms of the rock bss with those of Aroclor 1242 and 1016. There.are no distinguishing features which can reliably determine whether Che mixture in the fish is Aroclor 1016 or 124?. FIGURE 20 presents the mass chromatograms of the pencachloro PC3's and shows that only trace amounts of the pentachloro PC3's were present in the fish sample. The number of. isomers of pentachlorobipher.vls is greater in Aroclor 1242 than in 1016. These same PC3 isomers occur in the roc!c bass extract suggesting that the fish contain an Aroclor other than 1016. TABLE 6 presents the PCB concentrations as Aroclor 1242 in the samples below the discharge. The data shows that the PC'3 concentration ranged from 73 ug/gm ia the minnows to 350 ug/gm in the composite sample of tvo bass. The PC3 concentrations in snails were 45 ug/ga and 27 ug/gm at Station 1 and Station 4, respectively. Because of the dilution of the extracts for these analyses, the concentrations of other PC3 mixtures were not quantitated; however, the concentration of Aroclor 1254 was below 5 ug/gm in these samples collected below the discharge. V P Histopatholoev No noticeable differences were present in the histomorphology of tissues ^examined from fish collected from the__control^area (Station 0) and from the sampling site .75 miles below the General Electric outfall (Station 4) 11 HONS 0869b1 pcocMicroscopic araminazion uc kidnay, iivor, gonad i.yi :i.giCzvtt trace eid not show histological aberrations in -ither group TIGRIS 21 Co 23). DISCUSSION The PC3's being discharged are in sufficient quantities that con tamination of all environmental substrates have occurred; water, sediments and biota. ' Although an exact accumulation and transfer model could not be determined for the sampling area, a hypothetical presentation-is given below: BIOTA (AdsorDtion) n. ^Gra^in*? <v Tn'jpcrtn\ - DISCHARGE => AQUEOUS PRASE ------------------------ > PLANKTON & ----------------------'3- C ' > s? JAILS EP1FAUNAL CC^PJNXTXES (Adsorption & Sed imentation) Ingestion) \y SEDIMENTS SHIVERS, YOUNG PERCH (Ingestion) ROCX^BASS At all stations the biota has higher levels of PCB's than the back ground water concentration. At all stations, except Station 3, the biota contained higher levels of PCB's than the sediments. , t *' . At all stations the snail populations are important accumulators'and concentrators of PCB's (TABLE 7). The species collected are primarily herbivorous grazers, living off Che periphyton growing on rocks, lumber slabs and benthic surfaces. The snail3^probably ingest sedimented materials containing adsorbed PCB's along with their natural food. 12 MONS 085962 ;Sl5 ouncer, -;rML.- \ 1 . i : 21 1 jc-.-.v* .Tr-i be hi :m.v*r:if led vichin :h; ljou web -, v - c c . ,. . 1; [ r cu,,.. jrctuencs of larger game fish. This ij a oossij La prchva-- far biomazniilcucion Likewise, the PCB's are remaining environmentally active end are noc becoming deactivated by the geologic sedimentation process. A confusins_issue is that Aroclor 1015, although definitively- analvtad in water and sediments by computerized CC/MS, was not clearly discerned in the biological tissues. This_problem is particularly bothersome for interpretating the meaning of the contamination levels relative to Section 504 (FWPCA). IE the PC3's in the biological tissues could not be identified as Aroclor 1015 which supposedly is the only PCS now being discharged by General Eleccrtc at Che Hudson Falls and Ft. Edward facilities, then what other possible source might there be? It is possible from the variety of industrial uses documented, that ?C3's are being discharged into Che Hudson River upstream of the General Electric plane. From the contamination levels in sediments at Station 0, ..other sources of PCB's, even Aroclor 1015, exist in the Hudson P.iver. ' It is entirely possible that the paper companies operating upstream are discharging PCB's. These compounds are found in printing inks as well as non-carbon copying papers and recycled fiber paperboard; averaging 6.4 ppm (Trout, 1972). . The PCBrlevels, expressed as whole body residue, in the minnows collected from Station 0 (7.0 ug/g) and Station 4 (78 ug/g) were noc sufficient to elicit a histomorphological response in the visceral tissues. Hansen e al^ (1974) reports that pinfish (Lagodon rhomboidas) exposed to 10 ug/1 of Aroclor 1016 for 42 days showed no histopathologic response in visceral tissues as compared to the control fish. The body burden of PCS's in the exposed fish was 111-170 ug/g wot weight; significantly greater 13 HONS 08 :>;r zm t.;:^ows . :rm Szazi::'. From cne l i t^racura search, vs could no-: ri.ru: oc^sntatLo'A oc chi boOv burden Isvelj of FCS's nec.'i.j.jry to elicit a hiscooacholoLcji response Ln. the coiwnon shiner (hotroo is cornucus cornu!1::). The PC3 level in Che Perea flavescens (Yellow Perch) at Station 0 is 63 times greater than that found in the same specie* from Lake Erie (Kelso Frank, 1974). The fish analysed in the Lake Erie study vers older and larger than those collected in the Hudson River. The most probable route of contamination for fish is through the dietary pathway and direct dif fusion across exposed gill, intestinal ana integument surfaces. If the perch collected at Station 0 were older and larger, their source of PC3's might be the snails, however, small perch are maLnly plankton and epifauna consumers (Tharract, 1965). It Ls significant that the shiners and snails both contained PC3's, especially since these species are important grazers upon periphyton ccrrmunities and serve as food for large consumers, namely the g3toe fish; i.e., pike, pickerel, bass and larger yellow perch. The PCS.level in the rock bass is greater-than the maximum level documented for fish taken from any industrial river of the U.S. (Nisbat & Sorofim, 1972). This represents a new record for PCB contamination of fresh water fish. Although the game fish species are not commercially utilized in this stretch of the Hudson, sport fishing is a common widespread recreational activity for the inhabitants of Hudson Falls and Ft. Edward. Undoubtedly, the area was utilized to a greater extent prior to Che destruction of the impoundment. Certain areas, particularly downstream from Station 4 are 14 MQNS 085964 * ' jriMr'.l? by t:. '/Junijjc ,1: - . cuv.-irc. . ji r: j: :h*se ::: the poauius would certainly lead Co r-'nsamir.acion of tissues til L- bodies as documented by previous Lovascisscoc:; mentioned in tbs introductory section or this report. COMO-USIOMS 1. The General'Electric facility at Ft. Edvard is discharging large concantraeions of Aroclor 1016 with oil and grease. The high levels of con ' tamination in the downstream sediments is probably from the Ft. Eduard outfall. 2. Other sources of Aroclor 1016 are present upstream from the General Electric outfalls. " * . 3. The biotic components of the river ecology are heavily contaminated with PCB's, grazing populations as well as carnivorous piscine populations. The exact source of the PCB contamination ip the upstream contamination is unknown. ' For the downstream communities, the contamination is probably from the General Electric outfalls. A. Min could possibly become contaminated by ingestion of piscine populations taken from the sampling area. . 5. The General Electric facility at Ft. Edward is a contributory source of ' PCB's to this wacerway, and not a sole source. * RECOMMENDATIONS ' ' '' . ' Depending on how enforcement action proceeds, it nay be appropriate to obtain additional data on the distribution of Aroclor 1016 in fish, snails and sediment above and below the General Electric's outfall. Also, an investigation of other sources of PCB discharges should, be conducted to delineate the extent . of contamination in the Hudson Falls areas.- 15 MONS 085965 Biota Collected During the Hudson ?.iver Investigation (Hudson Falls-rt. Edvard Area) Location Station 0 Piscine Nana Motoois cornutus frontalis (Agassiz) (Northern Common Shiner) Perea flavescens (Mitchell) (Yellow Perch) Number 31 Gastropod Name Helisoma sp Number 35 4 Phvsa so 1 Station 1 (Above Outfall) None Station 3 Amblooletes rut>estri3 ruoestris (Rainesque)" (Northern Rock Bass) Helisoma sp Phvsa so 4 24 2 None Station 4 Notropis cornutus frontalis 42 Helisoma sp i (Agassiz) Phvsa so 60 (Northern Common Shiner) Limnacea sp 19 t i 16 HONS 085966 Analysis of Water and Sediment Samples for Aroclor 1015 from the Hudson River in the Area of Fort Edward, >!ev York Location Station 0 Station 1 Station 2 Station 3 Station U Contain:.nation Levels Water ug/1 Sediment mg/kg (opM ( oral < 1.0 2S00.0 6.9 6700.0 2.2 540.0 (3.0)* (3.1) 29S0.0 < 1.0 6.6 ^Results of replicate analysis - a quality assurance procedure. tl*> I .iwX t 17 MQNS 085967 TABLE 5 PC3 Concentrations in Snails and Fish Above Baker's Falls (Control Area) on the Hudson River Region 11 Lab Number 46031 46031 46031 Sarnole 4 Yellow Perch 131 Shiner Minnows Snails .(conposite) PCS Concentration (uz/im-vet) Aroclor 1254 Aroclor 1343 Total PC3 4.0 13.0 17.0 2.0 5.0 ' -7.0 0.3 1.6 . 1-9 TABLE 6 PCB Concentrations In Snails and Fish Below the General Electric Discharges on the Hudson River Region II Lab Number 46035 46036 46033 46036 Sample 1 Rock Bass 42 Shiner Minnows Snails (Composite) Snails (Composite) PC3 Concentration (ug/gm) (as Aroclor 1242) 350 78 ' . 45" 27 IS NONE 085968 TA3L2 7 Concentration Factors o PC3's in the Hudson River Location Station 0 Station 1 (Above Outfall) Station 3 Station 4 Substrate Snails Common Shiner Yellow Perch Snails Rock Bass Snails Connon Shiner ' Concentra tion rector Tissue/Wacer Tissue/Sed1menc 1.9 X 103 7.0 x 103 17.0 x 103 3.63 .98 .41 45 x 103 117 x 103 27 :t 103 7S x 103 6.52 .12 11.32 4.09 19 HONS 085969 Anon . , 1971. Water Resources Data for New York Part 1. U.S. CeoLogical Survey, 311 pgs. Surface Water Records, Cacti, K.C., Harris, S. J.t Bitman, J. & J. F. Fries, 1973. Polychlorinated biphenyl induced decrease in liver vitamin A in Japanese quail and rats. Bull. Env. Contam. Toxicology 9_:179. Grant, D. L., Villeneuve, D. C., McCully, K. A. & W. E. J. Phillips, 1971. Placental transfer of polychlorinatao biphenyls in the rabbit. Environ. Physiol. Iy6l. Hansen, D. J., Parrish, P. R., and Forester, J., 1973. Aroclor 1016 : Toxicity to and uptake by estuarine animals. No. 172, Gulf Breeze Environmental Research Laboratory. Concritrttion ' Kelso, John R. S Richard Frank, 1974. Organochlorine residues, irercury, copper and cadmium in yellow perch, white bass and stnallmouth bass. Long Point Bay, Lake Erie. Trans. Amer. Fish. Soc. 103(4):577-58l. Kimbrough, R. D. 1974. The toxicity of polychlorinated polycyclic compounds and related chemicals Critical Reviews in Toxicology (4):445-493. Nisbet. I. C. T. & A. F.'Sarofin, 1972. Rates and routes of transport of ?C3's in the environment. . Perspectives, Experimental Issue, April 1972. Env. Health Price, H. A. & R. L. Welch, 1972. Occurrence of polychlorinated biphenyls in*humans. Env. Health Perspectives, Experimental Issue No. 1, April 1972. Tharratt, R. C- 1959. Food of yellow perch/ Perea flavescens (Mitchell) in Saginau Bay, Lake Huron. Trans. Araer. Fisheries Soc. 88(4).*330-331. Trout, P. E., 1972. PCB end the paper industry - a progress repot. Experimental Issue No. 1, April 1972. Env. Health Perspectives r > 20 MUNS 0fl5970 Tabia 2. PCS Cor.cantrasions in Snails rind 'ij>. Below tha 0.2. Discharges on the Hudson .liver Region II Lab i^uaber Saniola PCS Cr.cs?.u:2:ior/'.:i/~mj (ai Aroclor IS-2) 4&035 46036 1 Rock Bass 42 Shinar Minnows 330 75 46033 46036 Snails (Composite) Snails (Composite) ' 45 27 MONS 065971 r- FIGURE 2. Control Station (Station 0). Note emer cent vegetation and lucoer slab deposition i foreground. FIGURE 3 Cutfall struc ure fron General Electric facility - Ft Edward, liew York. ! - (V K-. MONS 085973 FIGURE 6. Northern Rock Bass (Anoioolites r. rupestris) collected at Station 3. FIGURE 7 Bottom deposits at Station 3 of fibrous materialj carried from an unidentified upstrean source. HONS 085975 FIGurlE 8. Coaaon Shiners in seine net collected ac Station 4. HONS 085976 FIGURE 10. . . ,, Confluence of General Electric effluent strc ar.d Hudson River. iiots formation of oil on vatar slick. figure' ii- j ' '.liter saiepling at confluence o General Electric effluent'and Hudson River- Mote oil on water slick formed from effluent. HONS 085977 FICu?o 1.1 Sludge banks in background virh r.swly cut river channel at Station 1 (outfall site). MONS Uflb97a FIGURE 14. Upstream view from Station **. Mote tree in photo center growing on log-rock crib in dicating level of water during impoundment. MGNS 085979 ? ; i' MQNS 085981 MONS 085982 ICU7.S 13 Comparison of the total ion current of the rock bass Aroclor 1242 and Aroclor 1016. 'act vitfc those of . "" MOWS 085983 icuas i? Comparison of the trichloro PC3 mass ehromaco3r3ms of the rock bass extract with those of Aroclor 1242 and Aroclor 1015. HONS 065984 5-Ci PC3s \ i 1 i T~' ' l f 1 - ' 59 1\7Z3 WCD 1213 &>. . *. J I" * ' * ` ` ` ......................* ' 1.................... 10 K0 :*vtl ?5i r rrm hrm aw ^ _ ^ * - r* ISO. SCO fS . . 133 . ' r* - ttS : * tsa i 733 i D-f-- * c ' ro I, -< i . *1 . . . '` | 1 * * * * 1 ' "I . . lea . iro # t=o Kcmrs nroo taw * CURE 20. Comparison of the pentachloro PCB's nass chromatograms of the rock bass extract with Aroclor 1242 and Arcelor 1016.* *' MONS 085965 rt (W FIG(jR 21. Cros$ section of liver tissue encoroassin ' digestive tract from cocnon shiner coliec at Station 0 (control). Kota nomal * tubulQsicusoidal structure 63 X. . | j. MUNS 065966 FIGURE 23. *' Cross section of liver tissue encompassing digestive tract, fror. coonsa shiner collected froa Station 4.' "Tiote normal tubuiosinusordal structure- 63 X. - * ; FIGURE 2-f Cross section of shiner collected tubulosinusoidal .ver tissue rrcn ccrrooc : Station 4. 'iote normal .rutture. 400 >:. MONS 085967 FIGURE 25 Crc33 section. of hidr.ey icon cotscon shiner collected at Station'd. 63 a. ! FIGURE 26. Cross section of sill branched chather froc co.tr.on shiner collected at Station t.. Mote corral gill filcaencs and lamellae. 63 X- MONS 065966 FIGURE 27 Cross section of kidrs y tissue fron common shiner collected at S ction 0 (control), Mot; noma! structure of p' eitimal tubules. 63. 2C,, HONS 085989  a in Fort *d\vard yUlayq-* cii oiaie jJociv ,. Dale Afjcnc y PCB-1016 (ppb) Flow (c/s) 8/13/74 12/4/74 1/14/75 2/11/75 3/11/75 4/8/75 4/28/75 8/11/75 E PA 2. 2-3,1 (2.77 mean) DEC 3. 0 DEC 2. 5 DEC 1. 3 DEC 1. 5 DEC 1. 5 DEC 1. 7 ' DEC ' 1. 5 3.280 .6,000 7. 000 5,000 5, 410 3,160 11.100 3.120 Pounds Per Dav ' 10 49 97 94 35 <A 26 102 25 -'All upstream samples collected at the Portland Cement Bridge in Glens Falls contained less than 0.1 ppb ol PCB-1016. KXllXBIT "D" HONS 085990 Summary of Preliminary PCB Results# of Upper Hudson River Samples in Vicinity of Hudson Falls & Ft. Edward Status as of October 8, 1975 T66590 SNOW . Station August 20 cfs Ppb (?/ Da y August 21 cfs ppb iif/Day August 22 cfs ppb #/Dav Auigust 23 cfs ppb {/Day A ugust24 cfs ppb f,l Dny HR5 - Rt. 9N 2610 no sample at Luzerne 2560 sampl e lost 2590 0. 3 4.2 2580 < 1 378 <0.05 <0.1 HR 1 - Portland Cement Co. Bridge <1 <14 no sample sample not analyzed . <-1 <0.1 <0.1 HR 2 - Upstream Bakers Falls <1 <14 sample not analyzed . < 05 <0.7 by EPA . <1 <ro.i <0.1 HR 3 - East channel below Ft. Edward <1 <14 HR 4 - Schuylerville no sample sample lost 0.3 4.1 0. 06 8. 4 1. 4 19. 6 <1 0. 2 2. 8 0.2 0.4 0.1 0.2-U i\ e suits. reported a s Aig/1 of A rod or 1016 $ samples colie cted by DEC staff during intensive sampling of GE plants at Hudson Falls and Ft, Edward; analyses at EPA, Edison, New Jersey laboratories. SUMMARY 07 UPPER HUDSON RIVER SEDIMENT SURVEY Status as of October 8, 1975 Station i 1 R MI I.D. No. Date Results Collected Received Aroclor Cone. - vLe/l Dry.Wi. 1016 I 1221 j 1254 | chroon River below 'arrensburg Board & aper ludaon River @ Lake iiizerne, NY Rt. 9N Br. (udson River above orinih (V) 0. 6 11PCB501 9/10/75 10/6/75 223. 2 11PCB601 8/28/75 10/1/75 218. 8 11PCB111 8/28/75 10/1/75 2. 4 <0.5 <0. 5 00 1. 2 0 0.2 1.2 fud'fton River below `orintb (V) and IPCO abCvc Spier Falls Dam) . 214.0 11PCB502 9/10/75 10/1/75 1. 8 0 0.1 'udson River above Herman Island Damor-c Sample udson River above Icais Falls Landfill udson River below lens Falls Landfill 209.9 11PCB803 9/30/75 205. 7 11PCB604 8/27/75 10/1/75 204. 8 11PCB605 8/27/75 10/1/75 0. 8 0 0. 03 1.1 14. 9 0. 8 udson River @ Portland ement Co. Access Road U. *32) 198. 9 11PCB602 8/27/75 udsor. River above akers Falls Dam 197. 3 11PCB603 8/27/75 10/6/75 0. 6 2.0 2.2 Comments EXHIBIT SUMMARY OF UPPER HUDSON RIVER SEDIMENT SURVEY Status as of October 8, 1975 Station 1 R MI I.D. No. Date Results Collected Received Aroclor Cone. - jus/g Dry Wl. 1016 | 1221 i 1254 | Comments frsen River below Bakers is Dam - core sample 196.2 ILPCB802 3scr. River at Ft. Edward 194.2 11PGB606 197 Bridge 9/30/75 8/27/75 10/6/75 10 13 5 Preliminary; earlier results of 9/30/75 59 ug/g (1016), 4 ug/g (1254 'son River above >mpson Island Dam i sample 1-son River above uylcrville at 4 Bridge 188.4 UPCB801 9/30/75 183.5 11PCB6U 8/27/75 10/6/75 10 13 5 Preliminary Tson River above .erford (V) 157.6 11PCBU3 9/5/75 10/9/75 3 16 3 o z V) c OvSr <> >C SUMMARY OF UPPER HUDSON RIVER SEDIMENT SURVEY . Status as of October 8, 1975 MONS 0 8 5 9 9 4 Sla tion ; 1 R Ml I.D. No. Date R esulls Collected Received Aroclor Cone. - juu/c Dry Wt. 1016 | 1221 i 1254 | Tributaries loo's ic River (c? North ^owr.al, Vermont loosic River below Hoosic 'alls, New York 4. 2 11PCB503 9/11/75 27. 6 11PCB504 9/11/75 Valloomsac River above forth Hoosic, New York 5. 0 11PCB613 8/28/75 '/alloomsac River below forth Hoosic, New York . 1.1 11PCB612 8/28/75 liens Falls Feeder Canal 71 South Street Bridge; ^ beve Glens Falls (C) - 11PCB903 10/1/75 llcos Falls Feeder Canal ' Jiury.oyRo Avc Bridge; , clow Glens Falls (C) and bovo Ft. Edward landfill 11PGB904 10/1/75 liens Falls Feeder Canal clow Ft. Edward landfill - 11PCB610 8/27/75 ;ld Champlain Canal above i. Edward landfill - 11PCB609 8/27/75 ild Champlain Canal below *t. Edward landfill and |)Ov<! Kingsbury landfill - 11PCB608 8/27/75 Comment s t . ' SUMMARY OF UPPER HUDSON RIYER SEDIMENT SURVEY Status as of October 8, 1975 - Station . ! R MI I.D. No. Date Results Collected Received Aroclor Cone. - iip./g Dry V.'t. 1016 I 12?.l i 125-1 | Id Champlain Canal below ingsbury landfill - 11PCB607 8/27/75 `hamplain Canal at New wAmp Road Bridge .`hamplain Canal at East trcct Bridge 6. 3 UPCB902 10/1/75 1.4 - UPCB901 10/1/75 Comments ONS 0 8 5 9 9 5 I'oiyclUol'lnated Biphenyl Analytical Results; Results of Analysis-bn Fisfi Flesh ' The chemical analysis of edible fish flesh, except where whole fish are indicated on the attached data sheets, for polychlorinated biphenyls (PCH's) ves conducted at three laboratories on those fish obtained at several stations given below. The immediate supervisor of all analysis at each laboratory is also provided. Laboratory location KPS Dept, of Environmental Conservation Rome Pollution Laboratory 8314 Fish hatchery Road Rome, NY 13440 Supervisor Earl J. Harris ' ' Water Hudson R. . ., . . ' . . . Station!a) Above Corinth Above Glens Falls Ac Ft. Edwardbelow G.K. 5 miles below Ft. Edward Stillwater Catskill-Trib. 193 Kingston-Rhineclifl Bridge Mouth of Wappingers Creek Mouth of Rondout Creek Mouth of Esopus Creek . Tappan Zee Bridge George Washington Bridge area NYS Dept, of Agriculture and Markets State Food Laboratory Laboratory building #7 1220 Washington Avenue Albany, New York 12235 Dr. Elmer George, Jr. Director Hudson R. Mohawk R. Rondout Cr. Waterford Little Fells Below Schenectady Bloomington KYS Dept, of Health Division of Laboratories and Research Critfin Laboratory Guildctland, NY 12084 Dr. Brian Bush Walloomsac/ Hoosic R. Walloomsac River in Hoosic Hoosic River above Johnsonville A copy of the analytical procedures used by each of the Laboratories is attached as Appendix A. The calculation sheets, analytical reports and chromtitographs pertaining to the analysis of fish at the above stations are available at each respective laboratory for inspection. . The data relating to dates of collection, location of collections, species of fish and numbers of each species of fish analyzed, length and weight of each fish, identification of each sample of fish, the PCR content of the fish analyzed, and if provided, the oil and DDT content of the fish analyzed, is given on the attached tul-les numbered l thru 21 . HONS 085996  Comments on data contained in Tables 1 thru 21, Fish analyzed by the Rome Pollution Laboratory in the initial sampling (July 25 thru September 5, 1975) were weighed and measured by the Laboratory end* in most instances* by the Regional personnel who captured the fish. The lengths and weights reported on the attached data sheets are those obtained by the Rome Pollution Laboratory and converted to decimal form. This procedure was used so as to obtain consistency in reporting of these measurements. The lengths and weights reported on the data sheets do not necessarily correspond with those obtained and reported by the Regional personnel collecting the fish samples. This is due to shrinkage of the fish as a result of freezing prior to analysis. The length and weight information on fish analyzed by the Departments of health and Agriculture and Markets are the data reported by the P.egions collecting the fish samples and converted to decimal form where appropriate. Lengths and weights reported pn the data sheets for the second sampling of fish (September 16-18, 1975) are those obtained by the regional personnel and converted to decimal form, where appropriate. . MGNS 06599 7 :-vc: Date Collected imcilmouth base 8/20/75 /'tirrc*terua 8/21/75 JtIrleui * >. i t c sucker C.itos torajs 8/20/75 8/21/75 cllov perch ?'rc.i (lave- ii2LL) ` 3/20/75 8/21/75 Saco Analysis Resorted 9/2/75 location Above Corinth 9/2/75 Above Corinth 9/2/75 Above 1 Corinth Hudson sivo; PCS Analysis Results (Analysis by Rosa Pollution laboratory) Lab. Albany Irtroc. Ident. Tag Length Weight Mo. (Inches) (Pounds) PPM is Aroclor 1242//016 5s HudCoop 5 ------- -9.1 0.35----- -sp^O 9.2 PPM as Aroclor J25A___ Trace ?FM Total SCT/ PCS'i OCB Trace X Oil Ryssrks ________ Coeposite of Z 5s Hud Coep 4 5s Hud Cosp l 5s Hud Coop 3 5 s Rud Coap- 6 5s Rud Coap 2 5s Bud 17 5s Hud 18 5s Bud Comp 7 11.0 11.4 11.0 11,0 10.6 6.7 7.1 7.3 7.5 7.7 10.0 10.0 10.0 10.6 14.6 18.5 6.7 6.9 7.1 7.1 7.1 Trace Trace Trace Trace Trace Trace Trace Trace Trace Trace co Casposicc of 2 O. O ^ Composite of 3 O 2 Composite of 3 O X' Composite of 2 Composite of 4 Trace Trace Trace Trace Trace Trace Composite of 3 Date Collected sllrout: bass 7/24-25/75 - -te icui) : p'e rc h 7/2^-25/75 u svjCica; Date Ana lysis Reported Loestion Bucsoc River PCS Analysis Results (Analysis by Rose Pollution Laboratory) Lab. Albany Idcnt. Tdeot. Tag Length Weight No. finches! /Pounds! PPM ss ??H as Aroclor * Aroclor 1242//016 1254 Total PCS' s IA3I.1 2 jy/ DDT/ pcb 2 mi 6 6 6 S 8 0 SNOW 8/11/75 Above Glens Falls 3-Huc-75-up 4-Hud-75-up . 5-Hud-75-ap - 6-IIud-75-up 8-Hud-75-up 9-Hud-75-up 7-Hud-75-ap 10-Hud-75-up 1l-Hud-75-up 12-Hud-75-up 11.0 10.6 9.4 9.4 9.0 8.7 8.3 7.5 6.7 6.3 0.54 '0 0.46 0 0.35 0 0.31 0 0.30------- __o 0.21 ^ 0.26 0.16------- --^ 0 0.13 o.io-^ Trace Trace Trace Trace Trace Trace Trace Trace Trace Trace Trace Trace 8/11/75 Above Glens Falls 2-Hud-75-vp l-Hud-75-up 15.4 15.0 1.03 1.05 0 Trace Trace 0 Trace Trace S/il/75 Above Glens Falls * 16-Hud-75-p 15-Hud-75-up 13-aud-75-ep 17-Hud-75-up 14-riud-75-up 26-Hud-75-up l9-Hud-75-up 21-Hud-75-up 22-Hud-75-up 18-Rud-75-up 11.8 11.4 11.0 10.6 10.6 10.2 9.8 9.8 9.0 7.9 0,73 0.67 0.57 0.60 0.55 0.61 0.55 0.45 0.42 0.63 0 Trace Trace 0 Trace Trace 0 Trace Traec 0 Trace Trace 0 Trace Trace 0 Trace Trace 0 Trace Trace 0 Trace Trace 0 Trace Trace 0 Trace Trace 6/13/75 9/2/75 Above Glens , Falls Above Glens Falls 4l-Hud-75-vp 42-Hud-75-vp 0 Trace Trace 0 Trace Trace Date Collected alley* (S:i'r.~stcdicn vitrecn) 9/18/75 pcrca 5 iovescc-rs ) 9/18/75 Dace Analysis Reoortcd 10/1/75 10/1/75 Hudson River PC3 Analysis Results (Analysis by Rosa Pollution Laboratory) tail; 3 Location Lab. Idont. AC Fort 4-ft,d- Edward RI-Cosp below G.E. Albany Ident. Tag Length Weight No. (Inches) (Pounds > 6R8286 6RS235 6P.3288 6RS289 6K52S3 6R8284 6.4 6.6 6.7 6.3 6.9 7.2 0.12 0.12 0.12 0.12 0.16 0.16 PPM as Aroclor 1242//016 PPM as Aroclsr 1254 28.29 Total PCs's ?n-: tzz/ DCS 132.50 2.31 1, 0:1 P.:r? r 4.25 Ccsposite os 6 whole fish 3-HucRl-Coap 6RS230 6R8275 6R8282 6R8278 6RS276 6RS279 6R8277 6R8281 7.1 7.2 7.5 7.6 7.8 7.3 8.0 8.3 0.16 0.16 0.19 0.19 0.19 .0.19 0.22 0.22 41.30 122.92 7.05 5.52 Ccr.posita of 3 whole fish 3-HudRl-Comp 6R8291 6R8293 6RS292 6R8290 10.7 11.3 11.3 11.7 0.47 0.53 0.56 0.66 9.88 1.30 Composite of fish fillets At Fore 6-Hud- Edward RI-Comp- below G.E. 6R675 6R82 9 8 6R8299 6R632 6R673 6R8295 6R8297 6R688 6R8296 6R3294 6.3 6.3 6.8 6.9 7.1 7.2 7.4 7.4 7.7 7.8 0.16 0.19 0.19 0.22 0.22 0.19 0.22 0.22 0.22 . 0.25' 79.34 2.20 1.57 Cozposica ci 10 O o oo CO o z Q "Cf ti ck-bass '.-blertites Hudson liver PCS Analysis Results (Analysis by Rose Pollution Laboratory) Sate Collected Date Analysis Reported Location Lab. Albany Ident. Ident. Tag Length Weight Wo. (Inches) (Pounds) ?PK as PPM as PPM Aroclcr Aroclor Total 1242//016 .1254 ?C2'_s DCP Oil a-rt'srks 7/24-25/75 . S/13/75 Fort Edward 56 Bud-75-DR below C.E, 57 Bud-75-M Cospssite of 2 .emfa 7/24-25/75 ;~y--:uc?harcr.r::s) 8/13/75 Fort Edward S5 Hud-75-DH below G.E. . 20.35 MONS 0 8 6 0 0 1 1 Dace Collocrrd Yellow Perch (? f l.ivescens) 9/18/75 Date Analysis Rpscrce-6 10/1/75 Hudson Rivet PC3 Analysis Results (Analysis by 2om Pollution Laboratory) location Lab. Albany IdenC. Tag Length Weight No. finches! ('Pounds 1 F2H as PPM as Aroclor .. Aroclor 1242//016 1254 Total PCP.'f ??! CLC3Tl / X Oil Sf-rr.-r:-:* At Fort Edward S-HudBl-Coap 299.30 7.67 ' 5.70 Composite of 10 vhcle fish; not tagged . 7-HudRl-Coap 24.27 152.60 3.84 * 3.58 Composite of 20 whole fish; not tailed o >0 CO o A z Q r Stogies MtiXe sucker (Catostssus c;r-.T-c-rso-l) Pace Collectad 9/18/75- Date Acalysis Reported 9/30/75 Hudson River PC3 Analysis Results (Analysis by Rose Pollution Laboratory) Location Lab. Albany Idcnt, Ident. Teg. Length Weight Ko. (Inches) (Pounds) PPM as ArocLor 1242//016 PPM as Aroelo; 125& At Port Edwerd 1 HudRl-Coap 2 HudRI-Cocp 6666RRRR888822226666768S 66666RRRRR88888222227776732194 11.6 82.88' ---------------- ------- --=>* 111433..212. "^ 7.8 6.83---------------- ------------ --*. 8.11S. 86..56 ^ 48.57 2.72 lASLi 6 Total PC3`s ??:; DOT/ DC3 131.45 5.64 %. Oil ?r*rks 4Composite ef 11.55 0.4i 5.. dor.posite a; v HONS 0 8 6 0 0 3 Oats Collected Sate Analysis Resorted Location Hudson River PCS Jbllyiii Results (Analysis by Rom Pollution Laboratory) Lab. Albany I dent. Tdeoe. Tag Length Weight No. /Inches) PPM as ' Arcelor 1242/1016 ::-iisOwth bass 7/28-29/75 * ;;rertf-rus ' 'icrti*;:i 8/11/75 5 oi, below 23-Hud-75-BH Fort Edward 11.0 0.74 10C.27 :iiya 7/28-29/75 8/11/75 5 ni. below 24-Hud-75-DN Fort Edward 13.4 0.87 157.27 ?K as Aroelor 1254 22.64 20.74 Total ?CB's iry 3ST/ m 122.91 178.01 MONS 0 8 6 0 0 4 : cp sucker 7/28-29/75 7/23-29/75 3/13/75 9/2/75 5 mi. below Fore Edward 51-Hud-75-DH A8-llud-75-D.V 50-Hud-75-DR 54-Hud-75-W 5 oi. below 52-flud-75-DN Fort Edward 49-Hud-75-DR 51.39 47.57 22.05 20.80 29.74 28.03 8.66 8.13 38.60 36.16 Date Collected 9/17/75. Dace Analysis Reported 9/26/75 Hudson River PCS Analysis Results (Acelysis by Rose Pollution Laboratory) Location Lab. Albany Ident. Ident. Tag Length Weight No. (Irchesl /Pounds) 5 ailes 1 Hud-R- below Fort Coop-9-18 Edvard 2-Hud s Conp 9-18 6R7801 6R7602 6R7804 6R7S07 6R7SOO 6A7808 6R7306 6R7S05 6R7809 6.5 6.5 6.5 6.6 6.7 6.8 7.5 7.6 7.7 8 Kud-t Coap-9-lS PPM as Arodor 1242//016 PFM as Aroclor 1254 18.95 15.01 34.21 Total ?C3*s PPM DIT/ T)C3 103.26 2.37 50.55 1.71 122.39 3.90 X 0; 3.52 Cempc whole 3.53 Co=?o 4.52 Ccr.po who La taggs s00990 9/16/75 .9/17/75 9/29/75 9/29/75 5 miles 6R699 below Fore 6R697 Edward ' 6R699 65.697 5.70 5.56 10.89 0.35 12.69 0.41 1.14 1.-9 Date Co ll*crod iravn bullhead - 9/16/75 ' itrclurus ri'5ul3s;:s) `r.ila mucker 9/16/75 c.*T--r,-or.i) Dace Asalysis Reported 9/26/75 9/29/75 Rudsca River ?C3 Aealysis Results (Analysis by Rose Pollution Laboratory) Locitior. Lab. Albany Idene. Ideat. Tag Length Weight No. finches) /Rounds) P?K as Aroclor 1242//0L6 5 siles 3 Hud R belov Ft. Coop 9-18 Edvard 6R665 6R667 6R662 10.0 10.0 10.6 0.6A 0.66 > 0.63 ^ 73.24 4 Hud R Coop 9-18 5 miles belov Ft. Edward 7 Hud R Comp 9-13 6 Kud R Conp 9-18 5 Hud R Comp 9-18 6R669 6R698 11.3 11.4 6R696 6R680 6R663 6R677 . 10.2 10.7 10.9 n.o 6R694 6R683 13.0 13.8 6R691 6R661 14.0 14.5 0.78-----0.78-^ 65.30 0.59--------736.03 0.53 . 0.59 / 0.62^ 1.25 ^78.00 1.38-"-- 1.44 ^ 57.54 -------------- PPM as Aroc lor 1254 36.38 38.52 12.86 23.79 41.10 ZAZ~LZ 5 Total ?CE1 s dc:/ Z DC?. 109.62 4.23 123.32 3.32 48.89 1.35 Composite of 2 101.79 3.47 2.92 Composite or 2 98.64 2.99 5.31 Composite of 2 >O o o43 30 O <A Z O '"cries Cate CellecZei : jrsczcuth bus Hum "'liCrsaCCTUS " 9/17/75 -.t-Tican cel 9/17/75 ' ill."! ro s t r t a ) cvr. bu 1 Ihc j<i 9/17/75 r.ebulosus) 'MU sucker 9/17/75 'C-.Cos:c:r.uJi c: --ersoni) 9/17/75 Sudsoa Kiver PCS Analysis lesults (Analysis by Aoce Pollution Laboratory) TASLS 10 Sate Analysis Resorted 10/1/75 10/2/75 10/2/75 10/1/75 Location Lab, Albany Ident. Tdent. Tag Length Mo. finches) Stillwater 1 STO-Coap ' 2 SVH'Conp 3 SWR-Coep Stillwater Stillwater Stillwater 4J2970 4J2956 4J3000 4J2975 4J2961 4J2977 4J2935 AJ2972 4J2979 4J2971 4J2965 4J2974 4J29S4 4J295S 4J2957 4J2970 4J2956 4J3000 4J2975 4J2S61 12,4 12.9 5.7 5.9 6.0 6.2 3.4 4.2 5.2 5.6 9.6 7.7 17.0 32.0 9.0 Weight /Pounds) pm as Aroclor 1242//016 1.41------ 24.85 l.S3~-" 0.13------ --7 14.31 0.16 0.09 / 0.16^ 0.03------ --7-8.99 0.06 0.09 S 0.12^ 0.56 12.40 0.34 14.91 0.31 11.28 3.12 403.38 0.34 4.66 PPM as Aroclor 1254 15.97 9.84 Total PCS ' s 40.82 24.15 ?7M 237/ DC3 Z Oil Re-arcs 7.32 Cosposite of 2 4.09 Coapesite of 4 whole fish 6.99 . 15.98 2.42- Cozoositc of 4 whole fish 4.44 5.98 11.07 155.87 7.99 16.84 20.89 22.35 559.25 12.is 0.56 0.73 13.68 3.93 5.74 30.04 3.25 Very Oilly 10/2/75 Stillwater 5 SVH-Ccmp l 10/3/75 . Stillwater 4 SWH-Coap 4J2969 4J2954 4J2990 4J29S3 4J2964 4J2980 7.0 7.2 7.6. 7.8 13.3 15.0 0.16------ -^10.34 0.19 0.19 / 0.25^ 1.00-------3^154.28 1.63-"^ 5.94 16.26 ` 0.53 1.87 Corpositu of 4 35.92 190.20 5,69 3.71 Casposita of 2 MONS 0 8 6 0 0 7 i>iZ.C Col 1ecifd Useath bass crooteru s :i2i*il 8/26/75 8/27/75 ' 8/26/75 t sucker <t.o f torus ---TSOTU) 8/26/75 8/27/75 ' 8/26/75 8/27/75 iicye r~:z' stedlon S/26/75 Cadges River PCS Analysis Kesnles (Ar.alysis.by Department of Agriculture *r.d Markets) D.-.CC Analysis T5-:--<*r:ed T,oe * tier Lab. ..Albany Tci.-rrr. Tdrr.C. Taa length -Weight ?:<*. fUehcf) . fPouner' ??S as Aroc lor 1342//P16 PrK as Arcelor .125-'. 7c ta 1 PTVs 9/5/75 . ' 5S (1) 6R1437 6R1411 6R1433 6R1436 6R1421 9.9 ^ / 10. i ' ^X 10.1 ^X 10.5 .X^ ' 10.7 - 9/5/75 Waterford . 9/5/75 Waterford } 9/5/75 . Waterford 10692 ' := ;; 10693 ' . .. 10694 5S (2) 53 (3) 5S (4) 6R1419 6R1418 6R1417 6R1424 6R1429 6R1425 6R1426 6R1405 6R1432 6R1438 6R1409 6R1423 6R1431 12.9 13.3 14.0 ' n.o - ' ' ... ' ^ 11.7 .. . .X. 11.8 X 12.1 .X 12.5^ . . . ' 15.7 16.5 16.5 ' 16.5^ 10C3` 5S (5) 6R1716 6R1415 lY.s----------- " 14.5 41.5 17.5 . 53.5 23.2 12.9 43.9 32.4 TA3LZ 11 777/ rr? 7. o-:i _ Cocposicc of 6 o. o . >0 Coaposice of 4 o A z Cosposite of 6 ' Composite of 5 Cosposite of j Cicj; Usee Collected 11llruruth bass '. c r n; < -lor.ieui) 8/1/75 Lr.` pickerel a a. :*i2cr! 8/1/75 low oerch re i vp scens! 8/1/75 Cc porch rr.r.e r.meric.m a) 8/1/75 ` Cate Ar.s lysis Resorted 3/11/75 Kudtea River ?C3 Analysis Results (Analysis by Rose Pollution Laboratory) Lccation Lab. Albany tdent. Tdenc. Tag Length Weight Vo. finches! /Pounds! . PPM as Aroclor 1242//016 Catskill 26-Hud-4-75 Trib. 193 11.4 1.01 13.76 PPH as Aroclor 1254 Tota i pea's IA2L 12 JT/ ELI/ DCS X on Smirks S/il/75 6/11/75 Catskill 25-Hud-4-75 Trib. 193 Catskill 28-Hud-4*75 Trib. 193 29-Hud-4-75 8/11/75 Catskill Trib. 193 1 27-Hud-4-75 38-Hud-4-75 39-Uud-4-75 40-Hud-4-75 37-Hud-4-75 36-Hua-4-75 35-Hud-4-75 33-Hud-4-75 34-Hud-4-75 32-Hud-4-75 30-Hud-4-75 3l-Hud-4-75 17.7 1.76 8.68 7.9 ' 0.24----- -r=~4.18 7.5 0.19--*" 10.2 ii.o 8.3 7.9 8.7 8.3 7.57.1 6.7 6.3 5.9 5.5 0.61 8.47 0.34,^ 0.32 >30.67 0.32-*-- 0.38^ 0.33 > 23.74 0.28-^ 0.20 ^-14.20 0.19--"" 0. 14 ^___ 0.13 _>12.23 0.11"^ O o o >0 ` x> o Cossosite of tS) z Composite ef Cocposi-Ce cf Cor^oslte of Composite of rsrrf is Date Collected Striped bass ^srene ?a>:.cilus) 8/7/75 -argerouth bass 8/7/75 r`ica parch --r.-'ricara) 8/7/75 Ysjlcw perch ' 5/22/75 ilavescensl Date Analysis Reoorted 9/2/75 9/2/75 9/12/75 9/15/75 Location Kouth of RondouC Creek Kouth of RondouC Creek Kouth of RondouC Creek Kouth of Rondout Creek Hudson Rive; PC3 Analysis Results (Analysis by Rone Pollution Laboratory) Lab. Ident. Albany Tdent. Tag Length Weight No. finches! fPnundp) PPM as PPM as Aroclor Aroclor 1242//016 1254 2D219 2D218 2D219 2D218 10.4 20.4 0.44 3.23 0 0 4.03 7.01 2D241 2D238 2D242 Hud-RCoop 1A Hud-RCoop 2A Hud-R* Coop 4A 2D241 2D23S 2D242 2D237 2D236 2D240 2D223 2D233 2D230 2D220 2D226 2D228 2D231 2D234 2C8498 2C8496 2D212 2D210 2D2U 11.6 12.2 13.7 15.7 15.7 17.7 8.8 9.0 9.3 9.5 7.4 7.4 7.5 7.8 8.6 8.7 8.8 8.9 9.0 0.96 1.06 1.41 2.38 2.50. 3.67 0 0 0 0 0 0 0.31-s_ 0.31 >0 0.37 / 0.43 0.23 0.19 . > 0.22 / 0.24*^ 0.242s. 0.324 0.291 ^\>0 0.242 > 0.326^ 1.73 4.87 7.65 3.71 3.37 8.52 7.45 8.12 5.28 TA3LC 13 Total ?C3' s ??A DDT/ DCS I nu 4.08 0.49 7.01 1.37 . OT 0 9 SO SNOW 1.73 4.87 7.65 3.71 3.37 8.52 0.3S 0.40 0.89 0.57 0.79 1.13 7.45 1.70 7.28 Coaoosite of 4 8.12 1.60 6.22 Cosposize cz 4 5.28 1.08 Cosposite of 5 Dace recies______ Collc-ccad Ijr-ersuCh baa a 8/6/75 v.-:g.-coter-.is spiral res) 8/6/75 -.Vric-n eel 8/6/75 'triced boss ry*mne 5ti Ijs) '.'-.ico perch 8/8/75 8/8/75 Cate Analysis Reported 8/22/75 8/22/75 8/22/75 9/12/75 9/12/75 Mudaos River PC3 Analysis Results (Analysis by Rose Pollution Laboratory) 14 location Lab. Albany Idcnt. Idcnt. Tag Length Weight Ka. finches) /Pounds) PPM as Aroclor 12&2//OU ??M as Aroclor Total 1254_______ PCB's ??:; ZZXf DCS 7. , Oil P rV Mouth of Wappingera Creek 2C8402 2C8417 2C8408 2C8402 2CSA17 2CS408 12.5 14.1 19.0 19.73 10.73 17.99 4.01 3.35 5.19 23.74 14.08 23.18 HONS 0 8 6 0 1 1 Mouth of Wappingers Creek 2C84A9 2CS426 2C8423 2C3439 2C8409 2C8429 6.0___ __ 6.3 6.4 6.5 7.0 7.0-^ ^^>15.74 4.14 19.88 Mouth of Kappiagers Creek 2C8472 11.32C8487 11.4 2C8469 11.7 - 16.36 2C8483 2CS470 14.3 2C84S6 14.4 2C8484 15.2-----' 2C8483 17.0 =s~41.69 123.61 10.42 52.11 53.64 177.25 Composite of 3 >27.0 Mouth of Esopus Creek 2C3451 2D24 2C8451 26.9 2D24 14.2 0.94 14.11 0 4.84 6.62 18.95 6.62 0.86 4.95 Mouth of Esopus Creek Hud R-Coap 3A 2D42 2D43 2D44 2D47 6.5 6.5 6.6 6.6 0.150.17 0.15 0.17' 9.65 9.65 1.59 Coopcsite of 4 Sate Collected *3ric*n shad j'.GSS s^oidissi-a) 5/22/73. ~ 5/22/73 * 5/22/73 5/22/73 Dcte Analysis Resorted 9/18/75 9/23/75 9/12/75 10/1/75 Location Budsaa River ?C3 Analysis Results (Analysis by Rose Pollution Laboratory) Lab. Albany Idem C. Idcnt. Tag Length Weight Ko. finches) fPonndil r?a as Aroclor 1242//016 KingstonRhinccllfi Bridge 2C8363 2C8369 2C8370 2C83S3 2C8366 2CS364 2C3356 2C3354 2C3365 2C8355 KingstonRhinecliff Bridge 2C8371 2C8372 Kingston- , 2C8367 Rhinecliff Bridge Kingscon- Rhinecliff Bridge . 2C8363 2C8369 2C8370 2C8353 20336$ 2CS364 2CS356 2C8354 2C8365 2C8355 2C8371 2C8372 19.5 20.0 20.0 20.0 20.8 21.0 21.2 21.5 22.0 24.0 20.0 21.5 2C8367 21.0 2.18 2.82 3.04 3.17 2.88 3.23 3.39 4,07 4.20 4.82 3.30 3.37 3.19 Trace Ti'c Trace Trace Trace Trace Trace Trace Trace Trace 22.91 58.13 Trace 21.0 3.10 23.40 PR{ as Aroc lor 1254 Total PCS'* 2.52 6.91 5.29 2.98 3.83 3.23 39.56 38.14 7.78 L9.35 2.52 6.91 5.29 2.98 3.83 3.23 39.56 33.14 7.78 19.35 Trace 22.91 9.22 67.35 6.40 6.40 9.68 33.08 TAiLE 15 PPM CDT/ CCS Z. Oil Rc-.c:V< 0.57 1.50 1.49 0.39 O.SC C. 77 4.41 4.24 1.60 2.99 . * 1.32 3.52 0.65 MQNi 08601.! ir.d th Date Collected Kay 1975 Dote Analysis Reported 7/24/75 . Location fc>4soa River PC* Analysis Results (Asslysis by Rose Pollution Laboratory) Lab. Albany ----- L<jrt. r?Tag length Weight soT fine/,,1 ,wO P?H SS Aroclor 1262//016 Tappan Zee Bridge 206 259 256 254 269' 1 NT 22.8 24.4 24.8 32.fi 37.4 37.8 5.98 7.08 6.30 14.74 20.24 21.12 PPM 2 8 A'roclor 12S4. 37.80 6.75 7.93 * 3.25 6.S3 3.86 XABLE la Total PCR's ?e;.; SDT/ y.?, % . Oil Re-V-k 6.39 1.52 1.69 0.93 2.07 1.39 13.56 7.12 5.36 5.98 5.41 9.61 066013 <s> Z o Sate Collected --ebicit herring 4/27/75 vicar, shad 4/27/75 .aback herring 4/27/75 Dote Analysis Reoortpd Location Budsoa River PCS Analysis Results (Analysis by Roae Pollution Laboratory) Lab. Albany Idenc. Tdent. tag Length Weight c. finches* fpcundsl PPM as Aroclor 1242//016 PPM as Arocis? 1254 iota 1 PCVs ?7V DDT / DCS X Oil Ss-.arV:s 9/24/75 1 aile below Hud-R-EE George Wash Cottp 1 ington Bridge 9/24/75 1 taile above Rud-SH George Wash CoBp 1 ington Bridge 0 5.13 5.13 0.85 13.11 Composite of 2 fil ets obtained froa anglers 0 1.88 1.88 0.30 19.83 Composite of S filletS'Obcaincd from anglers ' 1 mile above Hud-SH Kingseon-Rhinc!- Comp 2 cliff Bridge 10/2/75 1 aile below George Wash ington Bridge. 0 . 1.56 0.32 ' 0.52 1.56 0.84 0.59 0.7 3.93 1.64 Cccposite of 3 fillets obtained froa anglers Roe 4* Q O oo O <z/) a x '>s;cs Dice Collected '.rCRCuEa bass '' 'lercnCerus -al-rciccs) 9/2/75 :(io- porch :l'\-c:cen?) vice sucker 9/2/75 9/2/75 Vallooeas/Koosic River PCS Analyst* Results a (Analysis by Dcpartaenc oE Health) rAsvE 15 Dace Analysis Rt-Porccd 9/11/75 9/11/75 9/11/75 Location Lab. Albany Idcnt. Id^nC. Tag Length Weight Ko. (Inches > (Prnncsl PPM as Aroclor 1242//P16 Hoosic River 32-P-2 above Johnsonville 32-P-2 731171 4K0753 11.2 13.3 0.81-----^.2.8 1.56----- 32-P-3 32-P-3 7B1138 7B1131 7B1132 14.5 16.2 16.5 1.69. 1.8l^*v. 3.19---------^2.5 . Hoosic River 32-R-l above Johnsonville 32-R-l 4K46S0 4K0759 4K4694 4K0785 Hoosic River 32-F-2 above Johnsonville 32-F-2 7BU67 7BX194 Ho Tag Wallooasac 32-P-l River in Wailoomsac . 32-F-3 32-F-l 7B1197 7B1177 7B1198 32-F-3 731193 4.1 5.7 6.6 7.8 12.3 13.7 14.3 10.6 10.9 13.1 15.0 0.03. . 0.10^\^ 0.12 ^>2.0 0.19-""'^ * 1.00 0.81 ""^>-0.7 0,81"" 0.39^___ 0.38 J>2.9 0.70--^ 1.20 1.3 PKS as Aroclar 125-1 2.2 Total 5.0 3.5 6.0 3.4 5.4 0.6 . 1.3 2.9 5.8 0.6 1.9 PPM DDT/ TC3 o 40 CO O </> a Coscosite of 2 Coapesite of 3 Composite of 4 Composite c f 3 Composite of 3 :-?-th bass T.sterus V-.icui) ; =i` !lcrtia8 Tv.iH ;) 9/3/75 9/3/75 sucker rj.r.i:; 9/3/75 . orappi* 9/3/75 : >~r:.*CU IStUSl .-.outh bass nr??rus 9/3/75 llohavk River PCB Analysis Results (Amlysis by pepertaenc < Agricolturc and Starke t T.ob. Albany 1 dCr. r Iriopt. 15 Length Weight nrc'.x-t'l VTA as AI?rouc2ihordn P?K is \Aroeler ?V. 9/9/75 Below 10933 Schenectady 9P-1 9/9/75 Below 10937 . 9F-1 Schenectady 9/9/75 Below 10936 Schenectady 9F-2 9/9/75 Below 10935 Schenectady 9R-1 10934 9R-2 9/10/75 Belou 11050 Schenectady 9P-2 11051 9P-3 710628 710630 710196 710642 710633 710634 710637 710639 710641 710646 710643 710644 710647 710648 710651 710659 710660 710662 710658 710653 710654 710652 710651 5H587 5R586 51L590 5K588 5H591 5H592 5H593 5E595 1.6 1.9 1.4 T? rr 3.7 2.7 3.0 dot/ Z res a Coaposite of 3 Coepesite of 6 OH Q >0 & Composite of 5 Cosposite of 5 Cocposite of 4 Composite of 4 ye >acecion D-tc Ccll-etci: 8/29/75 . * .. 0=ts Analysis Ec^rtod 9/8/75 Mohivk River PCI Analysis Result (Analyse by Department if Agriculture and Markets Lab. Tifr.C Albany r?enC. Taj :r. Lcr.St!i Little Falls 10802 10P-2 4X1038 4R30C7 4R3463 5B245 4X458 15.5 15.9 16.3 16.4 16.6 Weight Aroclor i::..V/01i ??M as Araclcr Total i:5&______ rr^'.< 9.0 13.9 7 Composite of 5 . . '.-ouch boss 3/29/75 .noui,! sucksr 8/29/75 --riorO -r. billhead ; * lurus - :!$) 8/29/75 9/8/75 9/8/75 9/8/75 10801 Lit.tle Falls 10800 10P-3 . -5B313 5B89 4R3059 5D661 5B237 10R-2 6WC753 6B0501 17.4 17.8 19.3 22.3 22.8 10.9 12.3 Uttl. Fill. 10803 10804 Little Falla 10805 10F-1 1GF-2 10F-3 6UC378 7X1053 6ST833 6MR271 6B0745 6B0497 6WC726 6MR461 7X1954 6WC768 6MR1S6 4R3262 4X907 4R3615 4R3298 6MR260 8.3 8.3 8.6 8.6 8.9 9.4 10.6 10.8 11.2 11.6 11.8 10.9 11.2 12.8 12.9 13.2 9.5 13.5 9.5 13.4 4.2 4.2 Cocpcsi.se of 5 n* s O >0 o Cocposite of 2 <A 2 Q X Cocposite. of 6 11.3 14.0 Cocposite of 5 Cocposite of 5 Dace ' Cci:r.-d vren^uch bass icrcoterus irias) 8/18/75 .c: suckers --jrcrsnni.) 8/18/75 n :.rvn'.:s L2i> 8/18/75 3a cc Analysis ".Tofted Lr*r * t > rn JLoudout Creek ?C3 Analysis Results (Analysis by Dcparrscot of AgricuUure and Ka.keCs) Lab. Idcnt. Albany tirnc. Tag UrSIh /Inches T height ??M as Aroc lor 9/5/75 Blooalagten 10698 8P-1 2D178 2D177 2D181 2D180 2D179 7.0 ------- 7.1 7.1 7.7 8.0'-- 0 10697 8?-3 2D175 18.7------2D174 20.0------- - . 0 9/5/75 Blootaingtan 10700 8P-3 2DL92 2D186 2DL89 2D191 2D190 14.8------- 14.9 15.0 16.5 18.6*^ __ 0 9/5/75 Blooaingcon 10696 8C-3 2D194 22.7-------2D193 24.0 20195 --------------- - --0 TA3LE 21 P7H as Arse lor Tacjl SOI/ Z 1.2 1.2 Coapoiica o 5 1.9 1.6 1.5 1.9 Q 1.6 >0 c/> Z Xa 1.5 Ccapoiice or 2 Composite of 5 ZCoapositc cf Ai'pf-.u'li A. Analysis Procedures and Tec:u:i quen * ` ' .. ' Con.scrvatJLon -i Koine. Laboratory. , bvpnttnu.-M ot i.n\, i t onn.onial ' . * ' 1, Extract oil on so:thlct* XI. Alkaline Hydrolysis . l. Weight cn. 1 g. sample I. Ad-* '.j -i : "I! !i ^ 3. Add l ml KOU 300g'20G mi il20 A. Heat lrt waterbath 5 min. @ 77C . 5. Shake1 stopper' return to waterbath. Shake 2x more In remainder of 30 rain. 6. Allow to cool 7. Add 10 ml ll20. 8. Add 10.00 ml benzene . 9. Shake 10 minutes on mechanical shaker 10. Remove AQ. lower layer ' . * IH. 11. Add anhyd. to dry benzene fraction Dchydrochlorination ' 1. Take 5 ml of benzene fraction 2. Add S ml benzene 3. Add boiling chip A. Add .25 ml DBU soLn. (57. v'v in benzene) Aldrich 13,900-9 1,5 diazoblcyclo (5,A,0) undcc-5-ene . 5. Place in boiling water bath until benzene is boiled off 6. Rotate tube while cooling to remove traces of benzene IV, l. Add 5 ml chromic acid reagent to tube (5 g. CrOj + 3 ml lt^O -f- 60 ml Cllj C00H. 2. Stopper and place in water bath 75 - 80cC for 10 min. 3. Cool in cold water A. Add 20 ml l^O'swirl to mix . 5. Add 15.00 ml hexane 6. Shake l minute or 5 minutes on mechanical shaker 7. Withdraw lower AQ layer . 8. Add 20 ml lf20 MQNS 086019 . , ; 'J,,* Shake 20 :cc. 10. Withdraw AQ layer ` 11. Add'20 ml lt20 - 12.' Shake 20 see. 13. Withdrew A<} layer completely 1A. Add Na2 SO^ to dry hexane fraction V, Chromatograph-on F..C.G.C. * Fish samples arc ground three times in a Hobart Food Chopper and thoroughly mixed in a Hobart Planetary Mixer. An accurately weighed sample of approximately 50 grams is then lyophilized. This freezedried material is then extracted in a Soxhlit Apparatus with hexane. MQNS 006020 *'' veil Io v i im led1 Iit.ny.I.S in I j; 11 _ IP. a;.y..i.n..l. s ifpmrot |,A;1 x i<!n.,?3 (b) Equipment - see PAM l 11 0-1.10 (c) Viiriim Aerograph .1.1)00 lias llhronnLngruph - IiC detector with tritium ' source. O' x 2 nun j.d. glass column wiLIi il.D'.'i Si: 30 oil Oll/BO Gas tlhrum l). Column tCmp. J.83C, Injector temp. 2U5C, Detector temp. 2U5C, Carrier gas Nj 3D ml/miu. Sample (a) (b) (e) li'ci'iUMt ion . Small .lisli under ,1V' - Remove iitiil discard head and viscera; griml with tails - three times thru grinder. Mct1.itim fish above .10" - Remove and discard head and viscera; cut lengthwise along backbone and grind one-half .of Lite fish - three times thru grinder.. hxtremcly large fish - Cut off caudal .section at anal opening, cut lengthwise along backbone and grind one-half of the section - three times thru grinder. . Extract ion PAM I 212.13a `' Weigh VI grams of prepared sample into a high speed blcndor jar ami add 2011 ml acetonitrile. Illend at high speed for 2 min. and filter through a funnel containing a loose plug of glass wool into a 250 ml graduated cylinder and record volume if). Transfer measured filtrate to n 1 L separatory funnel. Carefully measure Jill) ml lu-xane into the same graduate and pour into the separatory funnel. Shake vigorously 1-2 min. Add 10 ml saturated NaCl sola and about GO!) ml It-,0 and mix LhorniighJy for '1.5 see. I.ct layers separate, discard the aqueous layer and gen l,I y wash the so I visit layer with two 1111} ml portions of 11^0. Discard washings, transfer solvent layer In a 1UD ml glass stoppered graduate and record volume (I'J- Calculate the grams (G) of sample to be placed on Florisil column os follows: . U = S x ( F/T ) x ( P/100 ) (1) S = grams of sample extracted r " volume of acetonitrile extract filtrate T - total, volume (inl ll^O in sample + ml. acetonitrile added - correction .in ml for volume concentration) Contraction volume of S ml is used . I'm: 1111-95 ml 11-0/200 ml acetonitrile. In this equation the water content of fish my be assumed to be 8V,i. P = ml of hexane extract recovered . Column f.liroiiuito'/.i'auliv I'AM I 21i.llld. Prepare 22 mm i.d. column with the amount of activated florisil dictated by recovery studies. Top rioris.il with about J/2" anhydrous KajSOq. Prcwet column with 'll) ml hexane. Place Kudcrina-Dauish concentrator with graduated collection vessel under column to receive cluute. "' To the IDO ml glass stoppered graduate containing the hexane from the parti tioning step, add 15 gm anhydrous Ca-SOjj and shake. Do not let extract remain with Da^SI)i|. Transfer solution to Florisil column. Ilinsc graduate 3 times with 5 ml hexane and add to column. When solution has sunk into column, rinse sides of column twice with S ml. hexane, flute column at about 5 mj./m.in with 2DU ml t>% ethyl ethcr/hoxauc cluaut. Remove K-U apparatus and add porcelain boiling chips. Place glass trap on Lop and concentrate the eluant to 1U mi volume on the steam HONS 086021  2- bath. This is Ihu assay solution. ' (jin; Chi Miiiatw.raiihy . inject a !i u.l aliquot of tlie assay solution .into the.Gas Clwni.iotor.rapli iwul compare Die rotontioo t lines of the subsequent peaks wi tli those of n suilnbl c standard whose peak heights are witln'n j- in;; of tlie injected sample. Quantitate the peaks on the basis of theic peak heights. Co Ienlat ion . lU'snl.ts should be reported in terms of parts per million PCIi's in the product. . Let A - sum of heights of standard's peaks in millimeters It = sum "f heights of assay solutions peaks in millimeters C " nanogcams of standard represented by peak heights A ' Tilt'll ( ti/A ) x C. = nnuograms of I'CII . represented by the peak , hoi gilts in the assay solution . ` (2) I'rom equation (.1), G = grams of sample plced on Florist 1 column which is also the grams of sample represented by the 1U ml assay solution, ' bet Y = ui nf assay solution injected X, = C./lt), p.ins ol sample per ml assay solution or mg sample per ul of assay solution.' ` Then Y x V. = mi ll igrlims of sample represented in the amount of assay solution . injected (3) Combining equations (2) and (3J with the definition or TOI: t ll/A ) x C ,, ppm TCI! VxX in the product . ' .. ' John .1. tlaxstadt Associate Chemist 9/10/7 S IIVS Food' l-APfUATPUY Stale Carpun - ) aVoratery Bldg. +'[ 1*120 lashinglon Avcouo Albany, Ky 12236 MUNS 086022 Atfdlysi'S P roccdut* * And Techniques - Department of Health, Griffin Lnbovaton . Clean up procedure for tissue in PCB analysis Soxhlct extraction * * *'. 1. Weight 5 g of tissue which have been filleted, ground ami composited. 2. Mlx well with 15 g of anhydrous Na250^ in a 50 ml beaker. 3. Transfer to an extraction thimble, (25 X 80 am, single thickness). ` 4. Add 80 ml of hexane - acetone (2/1) mixture and some glass beads (about 50) to the bottom flask. 5. Sot the flask on the heating mantle with the soxhlec extractor and condenser in place. ' 6. Turn on the water and the power-stat. (setting at 50). Reflux for 12 hours. 7. At the end of refluxing, let the solution evaporate to 5 ml. 8. Add 25 ml of CrQ^ mixture (prepared by dissolving 9 g of . CrO-j in 6 mL of water and adding 50 ml of glacial acetic acid). Heat at 90-100 C for 45 minutes with stirring. 9. Tour the oxidation product into a 1/2 liter separatory funnel. Add ICO ml of water. 10. Add 25 ml of petroleum ether. Shake vigorously for two minutes and allow for phase separation. After removing Che aqueous phase, transfer the ox. phase into a. 125 ml flask. 11. Repeat the extraction (step 10) three more times with 15 ml of petroleum ether. 12. Combine the petroleum ether layer in the same separatory funnel, rinse the 125 ml flask 3 X 5 ml petroleum ether. Discard the aqueous phase. .. 13. Filter the petroleum ether solution through anhydrous Wa^SO^ to remove any moisture. Collect the filtrate in a Kaduma-Danish evaporator. . -- * 14. Evaporate to 2.5 ml. ' 15. Transfer the extract to a viai for gas chromatography analysis. MONS 086023 16. .Tin; vial is loaded into the j a* chrcruitogtaph ^ampler . * enrrouscL. The column Is 2V Apiezen I. on Car.-Chrome Q, G ft. column, temperature IMS C, i low rate 65 ml per minute. The .integrator ami computer have been calibrated with a mixture of 1221 plus 1016 plus 1256 using BliHC ns an external standard. . 17. The area of the first peak retention time 0.77 minutes is used to estimate 1221. The next 2 peaks (1.80 and 1.55 minutes) arc used to estimate 1221 plus 10LG. Teaks with retention 2.06, 3.17 and 3.0ft are used to quantitate 1010; "with peak retention time 6.63 used to estimate 1016 plus I256f and peaks with retention time 5.97, 7.61, 8.53, 10.95, 11.63, 13.89, 16.01 and 18.60 minutes are used to quantitate 1256. The mean of the estimate given by each-peak is used to determine the total quantity of 1016 or 1256. 18. Calibration is effected by computing unique peaks relative to n mass of 5.0 ug/g of fish and whereas combined peaks are calibrated with respect to a mass of 10 u/g of fish. The unique peaks give a better estimate of the quantity present than the combined peaks which are used only as a check on the analysis. 19. Results arc expressed ns ug/g wet weight of fish as Aroclor 1016 and as Aroclor 1254. The total PCB present is determined by adding these two estimates. MQNS 0060^4 PCD USER General Electric Niagara Transformer Corp. fork Capacitor Corp K. F. Interonics Axel Electronics AMOUNT USED (1974) 520,000 gallons 40,000 gallons 50,000-70,000 gallons 1,000 gallons 500 gallons EXHIBIT "C." MQNS 086025 Abrohainson, L. J. and J. R. Allen The Biological Response of Infant Monhuman Primates to a Polychlorinated Biphenyl Environmental Health Perspectives, June 1973 Allan, R., D. M. Morback and J. C. Hse Tissue Modification in Monkeys as Related to Absorption, Distribution and Excretion of Polychlorinated Biphenyls Archives of Env. Cont. and Tox. Volume 2, No. 1 , 1974 Allen, . R. Carstens La Barsotti Da - Residual Effects of Short-Term, Low-Level Exposure of Nonhuman Primates to Polychlorinated Biphenyls Tox Appl Fh 30(3) :440 74 19R A1 len, . R., L. J. Abrahamson and D. H. Norback Biological Effects of Polychlorinated Biphenyls and Triphenyls on the Subhuman Primate ' Env. Research, <5, 344-354 (1973) Allen, . R. Response of the Nonhuman Primate to Polychlorinated Biphenyl Exposure Research Activities at Regional Primate Centers Federation Proceedings, 34:8. July 1975 Allen, R. and D. II. Norback Polychlorinated Biphenyl- and Triphenyl-Induced Gastric Mucosal Hyperplasia in Primates Science, 2 February 1973, 179: 498-499 MGNS 086026 'EXHIBIT H' -2- Alien, J. R., and L. J. Abrahamson Morphological and Biochemical Changes in the Liver of Rats Fed Polychlorinated Biphenyls Archives of Env. Cont. and Tox., 1:3, 1973 Aulcrich, R. J., et al Reproductive Failure and Mortality in Mink Fed on Great Lakes Fish J. Reprod. Fert.. Suppl. 19 (1973), 365-376 Bagley, George E, and Eugene Croraartie Elimination Pattern of Aroclor 1254 Components in the Bobwhlte Journal of Chromatography 75(1973) 219-226 Bailey, S., and R J. Bunyan Interpretation of Persistence and Effect of Polychlorinated Biphenyls in Birds Nature, Volume 235 March 3, 1972, pp 34-36 Barsotti, D. A., R. J. Marlar and J. R. Allen Reproductive Dysfunctions in Rhesus Monkeys Exposed to Low Levels of Polychlorinated Biphenyls (Aroclor 1248) No date of reference given Barsotti, D. A., and J. R. Allen Effects of Polychlorinated Biphenyls on Reproduction in the Primate Federation Proceedings, 34, 338 (1975) MONS 086027 -3- . Bidleman, T. F-, and C. E. Olney Chlorinated Hydrocarbons in the Sargasso Sea, Atmosphere and Surface Hater Science Volume 183, February 8, 1974-, pp516-5l8 Bourquin, A.H. Cassidy S - Effect of Polychlorinated Biphenyl Formulations on Growth of Estuarine Bacteria Appl Microb 29(1): 125 75 N 10R Bruckner , J. V. and K. L. Khanna and H. H. Cornish Effect of Prolonged Ingestion of Polychlorinated Biphenyls on the Rat Fd, Cosmet. Toxical, Volume 12, pp323-330, Pegamon Press 1974 Bush, B., F. D. Baker, C. E. Tumasonia, Fa-Chun Lo and C. L. Houch Modification of the Homolog and Isomer Composition of a Polychlorinated Biphenyl Mixture During Passage Through Two Biological Systems ' International Symposium - Environment and Health CEC - EPA - WHO Paris June 1974 Cooley, Nelson J., J. M. Keltner Jr., and Jerrold Forester The Polychlorinated Biphenyls Aroclors 1248 and 1260, Effects on Accumulation by Tetratrymena Pyrlformis Journal of Protoroolegists, 20(3) 443-445 (1973) Dikshith, T. S. S., W. Rockwood, R. Abraham and F, Coulston Effect of a Polychlorinated Biphenyl (Aroclor 1254) on Rat Test Experimental and Molecular Pathology 22, 376-385 (1975) Fishbein, Lawrence Toxicity of Chlorinated Biphenyls Ann. Reviews of Pharmacology, Vol. 24, (1974) MONS 086028 -4- Halter, M. T. Johnson HE-Acute Toxicities of a Polychlorinated Biphenyl (PCB) and DDT Alone and In Combination To Early Life Stages of Coho . Salmon (Oncorhynchus-Kisutch) J Fish Res 31(9):1543 74 15R Hansen, D. J., P.R. Parrish andj. Forester Aroclor 1016: Toxicity To and Uptake By Estuarine Animals Environmental Research 7, 363-373 (1974) Hansen, D. J., P. R. Parrish, J. I. Lowe, A. J. Wilson Jr. and P.D. Wilson Chronic Toxicity, Upstate and Retention of Aroclor 1254 in Two Estuarine Fishes Bui. Env. Cont. and Tox., Vol. 6, No. 2 1971 Hansen, J. D., S. C. Sehimmel pgjd E. Matthews Avoidance of Aroclor 1254 in Shrimp and Fishes Bull, of Env. Cont. and Tax., Vol. 12, No. 2 1974 pp.253-256 Hansen, David J. Aroclor 1254: Effect on Composition of Developing Estuarine Animal Communities in the Laboratory Marine Science, Vol. 18, pp 19-33 1974 Hansen, D.J., S. C. Schimmel and J. Forester. 1974 Aroclor^L254 in Eggs of Sheepshead Minnows: Effect on Fertilization Success and Survival of Embryos and Fry. Proceedings of the Southeastern Game and Fish Comntission (In Press) Hsu, I.C., J. P. VanMiller, J. L. Seymour & J. R. Allen ' Urinary Metabolites of 2, 5, 2', 5'-Tetraehlorobiphenyl in the Nonhuman Primate (38999) Proc. Soc. Exp. Biology and Medicine, 150: S47-853 (1975) MQNS 086029 -5- Kimbrough, Renate D. . The Toxicity of Polychlorinated Polycyclic Compounds and Related Chemcials CRC Critical Review in Toxicology, Vol. 2, Issue 4 1974 pp445-498 Toxicology Review Lincer, Dr. Jefferey L. The Impact of Synthetic Organic Compounds on Estuarine Ecosystems. Nov. 1974, Research Report to EPA Gulf Breeze Lab. Nebecker, A.V. . Puglisi FA - Effect of Polychorinated Biphenyls (PCBS) On Survival and Reproduction of Daphnia, Gammarus, and Tanytarsus T AM Fish S 103 (4): 722 74 1SR Nebeker A.V. Puglisi Fa Defoe Dl-Effect of Polychlorinated Biphenyl Compounds on Survival and Reproduction of Fathead Minnows and Flagfish T AM Fish S 103 (3) :5G2 74 12R Nestel, H. & Joan Budal Chronic Oral Exposure of Rainbow Trout (Salmo gorrdneri) to A Polychlorinated Biphenyl (Aroclor 1254) Pathological Effects. Can. J. Comp. Med. Vol. 39 pp. 208-215 April 1975 Nimmo, D. R., R. R. Blackman, A. J. Wilson Jr., & J. Forester Toxicity and Distribution of Aroclor^1.254 in the Pink Shrimp Penoeus duorarum Marine Biology, International J. on Life in Oceans and Coastal Waters, Vol. II, No. 3 Nov. 1971 pp.191-197 MGNS 086030 Niireno, t). R?, P. D. Wilson, R. R. Blackman & A. J. Wilson Jr. Polychlorinated Biphenyl Absorbed from Sediment by Fiddler Crabs and Pink Shrimp Nature Vo. 231, No. 5297 pp50-52 May 7, 1971 Nimmo, D. R., & L. H. Bahner Some Physiological Consequences of Polychlorinated Biphenyl and Salinity - Stress in Penaeid Shrimp Pollution and Physiology of Marine Organisms 1974 Academic Press Inc. Nimmo, D. R., D. J. Hansen, J. A. Couch, N.R. Cooley, P.R. Parrish & J. I. Lowe. 1974. Toxicity of Aroclor ^.254 and Its Physiological Activity in Several Estuarine Organisms. Archives Environmental Contamination and Toxicology Vol. 3, No. 1, 1975 Ninino, D. R., J. Forester, P.T. Heitrauller and G. H. Cook. 1974. Accumulation of Aroclor L254 in Grass Shrimp fPalaemonetes pugiol in Laboratory and Field Exposures. Bulletin of Env. Cont. and Tox. (In press) Vol. 11, No. 4 1974 . * Norback, D. H. and J. R. Allen Chlorinated Triphenyl - Induced Extensions of the Hepatic Endoplasmic Reticulum Proe. Soc. Exp. Biology and Medicine, 139:41X27-1131 (1972). Parrish, Patrick R., David J. Hansen, John N. Couch, James M. Patrick Jr. & Cary H. Cook Effect of the Polychlorinted Biphenylj Aroclor10.16. on Estuarine Animals ASB Bulletin, Vol 21, No. 2, April 1974 p 74 MQNS 086031 Parrish, Patrick R., Jack I. Lowe, Alfred J. Wilson Jr., & James M. Patrick Effects of Aroclor 1254, A PCB on Oysters ASB Bulletin, Vol 19, No. 2, April 1972 p.90 - Parrish, Patrick R. 1974. Aroclor .254, DDT and DDD, and Dieldrin: Accumulation and Loss by American Oysters (Crassostrea virginica) Exposed Continuously for 56 Weeks. Proceedings of National Shellfisheries (In press) Platonow, N.S. & L. H. Karstad Dietary Effects of Polychlorinated Biphenyls on Mink Can. J. Comp. Med. 37 391-400 October 1973 Ringer, R.K., R. J. Aulerick and M. Zabik Effect of Dietary Polyelorinated Biphenyls on Growth and Reproduction of Mink American Chemical Society, Vo. 12, No. 2 pp.149-154 1972 Presented at 164th Meeting , Schinmel, Steven C., David J. Hansen and Jerrold Forester. 1974. Effects of Aroclor ^L254 on Laboratory-reared Embryos and Fry of Sheepshead Minnows fCvprinodon variegatusl. Transactions of the American Fisheries Society. Vol. 103, No. 3, July 1974 pp 582-586 Schimmel, Steven C., David J. Hansen and Jerrold Forester Effects of Aroclor 1254 on Laboratory-reared Embryos and Fry of Cyprinodon variegatus . . ASB Bulletin.21:2, April 1974,p.81. Stalling, D. L. & Mayer, F. L'. (1972) Toxicities of PCBS to Fish and Environmental Residues Environ. Itlth Perspect., 1^, 159-164 MOWS 086032 VanMiller, J.P., X.C. Hsu, and J.R. Allen Distribution and Metabolism of H-2,5,2',5'-Tetrachlorobiphenyl in Rats (38610) ' Proc. Soc. for Exp.Biology and Medicine 148, 682-687 (1975). Walsh, F. and R. Mitchell ' Inhibition of Inter-Microbial Predation by Chlorinated Hydrocarbons Nature Vol 249, pp 673-674, June 14, 1974 Walsh, Gerald E. Insecticides, Herbicides and Polychlorinated Biphenyls in Estuaries J. Wash Acad Sci, Vol. 62, No. 2, 1972 World Health Organization ' The Hazards to Health and Ecological Effects of Persistent Substances in the Environment - Polychlorinated Biphenyls. Report of a Working Group-Regional Office for Europe WHO Copenhagen (Printed 1975) 3-7 December 1973 Good overall review MONS 086033 Dy ) 941.6 TbJiV I. TABLE I Classifications and Standards of Quality and purity Assigned to fresh Surface Waters within the Upper Hudson River Drainage Basin in Essex, Hamilton, Warren, Washington, Fulton, Saratoga, and Rensselaer Counties, Rev York, Except Waters Contained within Boundaries of Scatc-evned Forest Preserve Lands, the Hooslc River Drainage Basin, and the Waters df Loon Lake, Brant Lake, Friends Lake, and their Tributaries. IIpm NO. Waltt* Index Numbi'' Name Description Map P.ef. No. Class Standards 1 H portion Hudson River and Champlain Canal from Trib. 240 (Hohavk River) to dam at Lock 2. Water source for the Town of Vatcrforch J-25SW J-25nw A A 2 X portion Hudson River and Champlain Canal From dam at Lock 2 to dam at Lock 3 J-25nv V D O > *u ri 73 a < 55 o>: o EXHIBIT " V 3 K portion 4 H portion Hudson River and Champlain Canal Hudson Klvcr and Champlain Canal From dam at Lock 3 to Trib. 301 (Batten Kill) From Trib. 301 (Batten Kill) to dam at Fore Miller H-25 J-25nw H-25 B D B 77 D Uni O 5 H portion Hudson River and Champlain Canal . From don at Fore Miller to Trib. 313 (Snook Kill) H-25 C C 6 H portion Hudson River and From Trib. 318 (Snook Kill) H-25 D D 3 O tzo Champlain Canal to 0.25 miles above falls at Glens Falls 0-25 7 H portion Hudson River From 0.25 miles above falls H-26 B 5 c at Clcns Falls to dam at C-25 a Spier Falls O' o U) 7a H portion Hudson River From dan at Spier Falls to dam at Corinth , H-24 C C 'SCi