Document Zj7BybgQnwoaGk06JEaOez77

AR226-3123 HASKELL LABORATORY FOR TOXICOLOGY AND INDUSTRIAL MEDICINE H-24108: STATIC, ACUTE 48-HOUR SCREENING TEST TO Daphnia magna PURPOSE: This is a rangefinding study designed to provide an estimate of aquatic toxicity using fixed test concentrations and data measurements at predetermined intervals. GENERAL INFORMATION WORK REQUEST NUMBER HASKELL NUMBER: SERVICE CODEf MATERIAL TESTED SYNONYMS/CODESp n p l -- -- M V J SPONSO|R>- ^"n'l^^pf'aaltifaCbfiBiicflJ^T NOTEBOOK IDj SUBMITTER'S I NOTEBOOK NUMBER COMPOSITION CONTAMINANTS CAS REGISTRY; PURITY MSDS No TEST CONDITIONS Nominal concentrations tested: 0, 0.5,1.0, 50, 500, and 5000 mg/L. Nominal Concentration (mg/L) 0 0.5 1.0 50 500 5000 Test Initiation (O hours) Dissolved Oxygen (ms/L) ^ ^ - PH At B 8.7 8.7 7.0 7.0 8.8 8.8 7.0 7.0 8.8 8.8 7.0 7.0 8.8 8.8 7.0 7.0 8.8 8.8 7.1 7.1 8.9 8.9 6.9 6.9 Test Completion (48 hours) Dissolved Oxygen (mg/L) ^ ^ PH At - 8.4 8.8 8.9 8.9 8.8 8.5 8.4 8.8 8.9 8.9 8.8 8.5s 7.4 7.0 7.1 7.1 7.2 6.9a Bl 7.4 7.0 7.1 7.2 7.2 6.9 + A and B represent replicate test chambers within a'dose level concentration. a Measurement taken on day 1 (24 hours) due to total immobility. Page 1 of 2 CttBipany SaniSteed. Does not contain TSCA CB1 H-24108: STATIC, ACUTE 48-HOUR SCREENING TEST TO Daphnia magna, CONTINUED RESULTS Based on visual observations, the water control solution and the 0.5,1.0 and 50 mg/L test solutions were clear and had no color throughout the test. The 500 mg/L test solutions were clear with a white suspended substance, and the 5000 mg/L test solutions were cloudy with a white suspended substance at test start. The white suspended substance in the 500 and 5000mg/L test solutions settled onto the bottom of the test chambers as a white cloudy film throughout the test. Immobilities were 0,0,10,20,0, and 100% in the 0, 0.5,1.0, 50, 500 and 5000 mg/L test solutions, respectively. Forty-eight hours after exposure, lethargy was observed in 25% of the remaining mobile daphnids in the 50 mg/L test solutions, and paleness in color was observed in 90% of the remaining mobile daphnids in the 500 mg/L test solutions. The 48-hour ECso, based on nominal concentrations and immobility, was between 500 and 5000 mg/L. All water quality parameters were within acceptable limits. CONCLUSIONS H-24108 exhibited low toxicity in a 48-hour, unaerated, static acute test using Daphnia magna. WORK/AUTHORED BY: ^(M^J^'^-J&CUtftPuJ^^ Laurie D. Bouchclle Toxicology Associate APPROVED BY: \oJ! i o ^ U. Paul W. Gnibe, B.A. Study Director n - ^ e ' w i Day-Month-Year iT-Ju^-fffl Day-Month-Year DISTRIBUTION: Page 2 of 2 Ccnipany Sanitized. Does not contain TSCA CBI