Document ZJYpjxBZwgxOx9pZY543MNgeZ

A R t t r O 3f l0 ACTIVATED SLUDGE RESPIRATION INHIBITION TEST TEST SUBSTANCE Identity: Perfluorodecanesulfonate, ammonium salt; may also be referred to as PFDS, FC-121-X, or 3M Sample E2112 and E2566-1. (1Decanesulfonic acid, 1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8,9,9,10,10,10-heneicosafluoro-, ammonium salt, CAS # 67906-42-7) Remarks: Test sample is a mixture of the test substance in water and 2propanol, 1-(1,1-dimethylethoxy). This mixture contains approximately 2226% test substance, 33-37% propanol, 1-(1,1-dimethylethoxy), 0.5-1.5% perfluorononanesulfonate, ammonium salt and 38-42% water). All values reported relate to this mixture. No calculations were made to adjust for the actual concentration of the test substance in the test sample. Test material was from 3M production lot 1. Note: Only the summary page was available for this study METHOD Method: OECD 209 Test type: Static acute GLP: No Year Completed: 1987 Analytical monitoring: Dissolved oxygen concentrations. Exposure Period: 30-minutes and 3-hours Measurements: Dissolved oxygen depletion rate after 30-minutes and 3hours of exposure. RESULTS Nominal concentrations: Blank control, 100, 180, 320, 560, 1000 mg/L, in duplicate. Element value: 30-min EC50 > 1000 mg/L 3-hour EC50 = 780 (630-1050) mg/L All element values based on nominal concentrations. Statistical method: Probit analysis Control response: The largest percent difference between control uptake rates was 7.5%; thus the two controls were within 15% of each other. 000208 Reference substance results: Not available Remarks: Testing was conducted on the "as-sold" mixture of the subject material (~22-26% subject substance in water). The value reported applies to that mixture and not the test material. No attempt was made to determine the impact of the presence of the organic solvents or what portion of the toxicity can be contributed to the perfluorodecanesulfonate. CONCLUSIONS The 3-hour EC50 for FC-121 was determined to be 780 (630 - 1050) mg/L Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY Reliability: Klimisch ranking 4. Most of the supporting material for this study is missing. Because a mixture was tested, care must be taken when interpreting the results. The solvent in the mixture could have enhanced or restricted biological uptake and/or toxicity of the fluorochemical component. The study lacked analytical confirmation of concentrations and characterization of the sample purity. REFERENCES This study was conducted by the 3M Company, Environmental Laboratory, St Paul, MN, Lab Request numberE2112, 10/30/87 OTHER Last changed. 5/18/00 000209 .' ENVIRONMENTAL LABORATORY ACTIVATED SLUDGE RESPIRATION INHIBITION TEST 1209 CALCULATION OF RESULTS Test Substance EC * > Lert I s) FC 30 - MINUTE EXPOSURE Test Substance Concentration (me'L) CONTROL T too ; : izo D : /e ro Oxygen Uptake Rate R O . M7 7 k . M ;a 4 Q r .45^17 C. O . 7 7.7 l c- . O t o H .V R C orrected^ to 20*C O 4776 ey. H2HQ O. 4 3 7 7 a .4.^77 0 .3 7 3 1 C- 3 / - 4 3 LR -All^ Date !Q ~3 ~S7 Analyst % Inhibition in Respiration Rate1 -- |3 I2 13 3 .S' CONTROL II r . ^coc 0 . 3 0 DO -- Percent Difference in Controls.(3) H. I' % BC^g (95% Confidence Limits) mg/l. 7 1O.C.'C mT"c 1L. 3 Test Substance Concentration (mg/L) CONTROL I fee ISC oL. Oxygen Uptake Rate R c S r to7 C, .5 i2 1 o .H l 1. 3 C. R Corrected^ to 2d*C 6 .5 0 to7 C.4 & 4 7 M 3 1v 1 Inhibition in Respiration Rate^"J -- 7 -G 7 c .... 33 . i S i `X2 O .3 3 3 3 7 <b.3 3 SiT 37 . . /reo o ,7 1 3 l o ac3 1 i-c -few - / c-*>ijt; o-, 3 /3 -3 - v _ j 2 . e. . - A c . ' .W. 7 o. s' y (at -- ^Srcent;D ifference in C o n tro ls^ 7 - i v o ______ A* (951 Confidence Limits) mg/l 7^0 ( (- ~ /p SOlffiiA.-Lfdoih T3 V(i)?Temp.- A rrtion s lobs v-V vJ; i'pr ^ V - - , - (mg 0,/L-m in.) (3) % Difference-_fL--ti ------^- 2ai-x 100 YRCj RC\ m W : N v (2;% Inhiiiitint 7 - Rs_ X 100 B ' =sf i ^ y ,,`R,(Oxygen in ta k e ra te (mg 0,/L -m inJ vT.obs Sanqple-itemp., C* & - 'r1.047,% X ^T.obs * 20* C C Control?.* '< r * f " it, j *4 * W > i3 7 - 0 0 0 210 The two control respiration rates should be within 15% of each other p^4_-fc>r f-s+ prep BEST COPY AVAILABLE