Document YDj9BVapKDJ29KzX3jgRe1R6y

Afi Jt%-053S MICROTOX TOXICITY TEST TEST SUBSTANCE Identity: Perfluorooctylsulfonate, didecyldimethylammonium salt; may also be referred to as Fluoroalkyl ammonium derivative. [1Decaminium, N-decyl-N,N-dimethyl-, salt with 1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8,8-heptadecafluoro-1 octanesulfonic acid (1:1), CAS # 251099-16-8] Remarks: The 3M production lot number was Lot 1. The test sample is L-14394 referred to by the test laboratory as P3025. The sample was labeled F-11615, Lot 1. The test sample is a mixture of the test substance in water (approximately 30-40% test substance, 60-70% water, and 0-5% of residual perfluorochemicals). All values reported relate to this mixture. The test sample appears to be a 2-phase dispersion (clear liquid with opaque solid) which rapidly separates after agitation. No calculations were made to adjust for the actual concentration of the test substance in the test sample. METHOD: Method: Azur Environmental Corp. Microtox "BASIC" Procedure Test type: Static acute GLP: No Year study completed: 1996 Year report completed: 1997 Species: Vibrio fischeriformerly Photobacterium phosphoreum Analytical monitoring: pH and osmotic condition Replicates: Two Statistical methods: EC50 values calculated by statistical linear regression program provided for Microtox Test organism source: Azur Microtox Reagent. Test Conditions: Dilution water: Azur Environmental Corp. diluent Stock and test solution preparation: Water accommodated fractions. Test solutions were prepared for each test concentration by mass addition of test substance in Millipore Milli-QTM water. The solutions were stirred overnight in the dark at ambient room temperature. A white precipitate was observed in the stock solutions after mixing. Prior to test initiation, a 10 mL aliquot of the clear liquid portion of each test solution was removed and each was osmotically adjusted. All test solutions were made by proportional dilution. Exposure vessels: 4 mL glass cuvettes. C0431 Number of replicates: Two Number of concentrations: Four plus a control. Element Basis: Percent light loss. RESULTS Nominal concentrations: Blank control, 125, 250, 500, 1000 mg/L Element value: 5-minute EC50 = 600 (580-620) mg/L 15-minute EC50 = 510 (500-530) mg/L 30-minute EC50 = 350 (240-490) mg/L All element values based on nominal concentrations. Remarks: Values reported are for the test sample. No calculations were made to adjust for the concentration of the test substance in the test sample. CONCLUSIONS The test sample 30-minute EC50 for Vibrio fischeri was determined to be 350 mg/L with a 95% Confidence Interval of 240-490 mg/L. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY Reliability: Klimisch ranking 2. Testing meets all criteria for quality testing, but lacks analytical confirmation of test substance concentrations in the test solutions and sample purity is not sufficiently characterized. Additionally, data is for a mixture and toxicity cannot be positively attributed to didecyldimethylammonium Perfluorooctylsulfonate salt alone. REFERENCES This study was conducted by the 3M Company Environmental Laboratory, St Paul, MN, Lab Request number P3025. OTHER Last changed: 5/24/00 004282 3M ENVIRONMENTAL LABORATORY INHIBITORY EFFECTS OF P3025 ON MICROTOX Azur Environmental Microtox System STUDY COMPLETED: December 19,1996 FINAL REPORT COMPLETED: May 12, 1997 Env. Lab Request Number P3025 Prepared by: Name: Title: Laboratory: Address: Susan A. Beach Advanced Environmental Biologist 3M Environmental Laboratory Building 2-3E-09 935 Bush Avenue St Paul, MN 55144 Microtox Page 1 May 12, 1997 SUMMARY The purpose of this study was to evaluate the inhibitory effect of P3025 on bioluminescent bacteria in accordance with the Azur Environmental Corp. Microtox test. The median effective concentration, 30-minute EC50 was reported as 350 mg/L. INTRODUCTION Toxicity tests with Microtox provide a rapid screening method and can be reliable predictors of the inhibitory effects of contaminants on microbial systems. This toxicity study measured the light output of bioluminescent bacteria over a relatively short period (5,15, and 30-minutes) in the presence of different concentrations of P3025. Test Substance: Appearance Aqueous Solubility Available Physicochemical Properties: Date Received Storage Test Solutions Reported values MATERIALS AND METHODS P3025, date 10/7/96, Lot 1 Two-phase dispersion (clear liquid with opaque solid) Apparently not completely soluble in water at tested concentrations. Boiling Point: Vapor Pressure: Vapor Density: Evaporation Rate: Solubility in water: Specific Gravity: Percent Volatile: pH: 100C ca. 18 mmHg @ 20C <1 <1 Insoluble ca. 1.2 60 - 70% 6-8 10/16/96 In the dark at ambient room temperature (22 2C) Test solutions were prepared as Water-Accommodated Fractions (WAFs). Four stock solutions of 250, 500,1000, and 2000 mg/L were prepared in Millipore MilliQ water. Each was capped and allowed to mix overnight, in the dark, at ambient room temperature. Test substance was apparently not completely miscible with water. A few white particulates were observed in each stock solution after mixing. Just prior to test initiation, a 10 mL aliquot of the clear liquid portion of each stock solution was removed and each was osmotically adjusted with 200 mg reagent grade NaCI. Each initial concentration was diluted by 50% during the course of the test. The reported values are based on nominal concentrations in the test media at the beginning of the microbial assay. 004234 Microtox Page 2 May 12, 1997 Controls: Blank control Exposure: Test Date Contact Time Vessels Number of Replicates Test Solution Vol. Starting Test Cone. Test Substance Dilution Water Temperature Lighting Two controls containing Microtox reagent (Photobacterium phosphoreum), but no test substance. 12/19/97 5, 15, and 30-minutes 4 mL Azur Environmental glass cuvettes Two 1.0 mL 125, 250, 500, and 1000 mg/L Azur Environmental Diluent (ultra pure water osmotically adjusted to 2% NaCI) Instrument temperature set at 15C Ambient laboratory light Measurements Procedure Light readings were taken upon reconstitution of the Microtox bioluminescent bacteria, and then 5,15, and 30-minutes after test substance was added to the bacteria. All readings were captured electronically via computer interface with the Microtox instrument. The test was conducted in general accordance with the Azur Environmental Microtox Basic Test. CALCULATIONS The reported values are based on nominal concentrations in the test media at the beginning of the test. The concentrations tested and the corresponding response data derived from the toxicity test are used to calculate, if possible, the medial effective concentration, EC50 ancl the 950/0confidence interval by standard logistic regression methods provided in the Azur Environmental software. The EC50 is defined as the concentration estimated to inhibit the rate of bioluminescence by 50% as compared to the controls, after 5,15, and 30-minutes of exposure to the test substance. 0<K2S5 Microtox Page 3 May 12, 1997 RESULTS The percent reduction in light output and the median effective concentration, EC50, obtained after 5,1 5 and 30- minutes exposure to the test substance P3025 are summarized in Table 1. The median effective concentration, 30-minute EC50 and 95% confidence interval was reported as 350 (240 - 490) mg/L. ACCEPTABILITY CRITERIA This toxicity test meets quality criteria provided by Azur Environmental Corporation: 1) The 95% confidence factor for the EC50 was <1.3 for the 5 and 15-min exposure periods. It was > 1.3 at 30-min; however, this was probably due to complete inhibition at the highest concentration tested (1000 mg/L). 2) The coefficient of determination was > 0.9 for the 5 and 15-min exposure periods. It was < 0.9 at 30-min; however, this was probably due to complete inhibition at the highest concentration tested (1000 mg/L) . 3) The correction factor was > 0.7 at 5-min and > 0.6 at 15 and 30-min. This new version of Azur Environmental Corporation software does not provide the correlation coefficient. This test was conducted by Trudy K. Stiefenhoefer as requested by Pam Wolf of 3M Specialty Chemicals Division. REFERENCES 1. Microbios Corporation. 1992. Microtox Manual. Volume 2. Detailed Protocols. Carlsbad, CA. 2. Microbios Corporation. 1993. Microbios Customer Training Manual. From February, 1993 workshop. 004286 Microtox Page 4 May 12, 1997 TABLE 1 SUMMARY OF TOXICITY OF P3025 TO MICROTOX PERCENT LIGHT LOSS* TEST CONC. mg/L 5-Min. Exposure 15-Min. Exposure 30-Min. Exposure 125 14 22 28 250 15 25 33 500 26 47 62 1000 95 98 100 EC50 95% Confidence Interval 600 (580 - 620) 510 (500 - 530) 350 (240 - 490) Statistical method used______ tour soitaaa_________________ ftzur software__________________________ A zur softw are * Mean of two replicates 004287