Document YDgB4G5V5zQzKGbnxXYDYXypK

AfW-ossH ACUTE TOXICITY TO AQUATIC INVERTEBRATES (E.G.,DAPHNIA) T E S T SUBSTANCE__________________________________________________ Identity: Perfluorodecanesulfonate, ammonium salt; may also be referred to as PFDS, FC-120, or 3M Sample J2905-1. (1-Decanesulfonic heneicosafluoro-, ammonium salt, CAS # 67906-42-7) Remarks: Test sample is a mixture of the test substance in water and butyl cellosolve (approximately 25% test substance, 37.5% butyl cellosolve and 37.5% water). All values reported relate to this mixture. No calculations were made to adjust for the actual concentration of the test substance in the test sample. Test sample was from 3M Lot 164. METHOD Method: O ECD 202. Test type: Static acute GLP: No Year Completed: 1992 Species: Daphnia magna Analytical monitoring: pH, dissolved oxygen, conductivity, temperature. Test substance concentration was not monitored. Statistical methods: EC50 values calculated using Binomial/nonlinear interpolation Testdaphnid source: In-house cultures, EnvironSystems Division, Resource Analysts, Incorporated, Hampton, NH Test daphnid age at study initiation: < 24-hours Test conditions Dilution water: Aerated, filtered natural well water Dilution water chemistry (from a representative sample): hardness: 166 mg/L as C a C 0 3 pH: 7.2 Conductivity: 540 pmhos/cm Stock and test solutions preparation: A primary stock solution was prepared in dilution water to yield a test sample concentration of 1000 mg/L. All test solutions were made by diluting the appropriate amount of stock solution with dilution water. Exposure vessels: 250 mL glass beakers containing 200 mL of test solution. Number of replicates: 4 Number of daphnids per replicate: 5 Number of concentrations: five plus a negative control 000136 Water chemistry during the study: Dissolved oxygen range (0-48 hours): 8.7 - 9.3 mg/L (control exposure) 8.8 - 9.1 mg/L (100 mg/L exposure) pH range (0-48 hours): 7.2 - 8.1 (control exposure) 7.2 - 8.2 (100 mg/L exposure) Test temperature range (0 - 48 hours) 19.4 -2 0 .8 C Element basis: mortality and immobilization RESULTS Nominal concentrations: Bk control, 15, 25, 40, 6 0 ,1 0 0 mg/L Element value: 24-hour EC 50 => 100 mg/L 48-hour EC50 = 44 (25 - 60) mg/L Statistical Evaluation: The EC50 value and 95% confidence interval was calculated using the binomial/nonlinear interpolation method. Mortality of controls: None Remarks: Values reported are for the test sample (mixture). No calculations were made to adjust for the concentration of the test substance in the test sample. Cumulative percent mortality and immobilization: Nominal Test Cone., mg/L 24-hours 48-hours Neg. Control 15 25 40 60 100 0 5 5 10 0 25 0 15 20 35 60 90 CONCLUSIONS The test sample 48-hour E C 50 for Daphnia magna was determined to be 44 mg/L with a 95% confidence interval of 25 to 60 mg/L. 0001.37 Testing was conducted on the "as-sold" mixture of the subject material in water (-2 5 % subject material). The values reported apply to that mixture and not directly to the subject material. Subm itter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY Reliability: Klimisch ranking 2. This study, while well conducted, lacks analytical data for: determination of the test substance concentration in the test solutions: and determination of the sample purity. Because a mixture was tested, care must be taken when interpreting the results. The solvent in the mixture could have enhanced or restricted biological uptake and/or toxicity of the fluorochemical component. REFERENCES This study was conducted by EnviroSystems Division Resource Analysts, Incorporated, Hampton, New Hampshire on behalf of the 3M Company. OTHER Last changed: 5/18/00 0001.38 J2<\oH Study Title Static Acute Toxicity of FC-120 to the Daphnid, Daphnia aagna Authors Timothy J. Ward Robert L. Boeri Study Completed February, 1992 Performing Laboratory EnviroSystema Division Resource Analysts, Incorporated One Lafayette Road Hampton, New Hampshire 03842 Page 1 of 14 000139 - $ V I. SUMMARY The acute toxicity of FC-120 to the daphnid, Daphnia magna, ia described in this final report. The test was conducted for 3M Company for 48 hours during February 5 to 7, 1992, at the EnviroSystems Division of Resource Analysts, Inc. in Hampton, New Hampshire. It was conducted by Jeanne Magazu, Peter Kowalski, Ellen Stanford, Robert Boeri, and Timothy Ward. The test was performed under static conditions with five concentrations of test substance and a dilution water control at a temperature of 20 1C. The dilution water was filtered natural well water .collected at Hampton, New Hampshire. Aeration was not employed to maintain dissolved oxygen concentrations above an acceptable level. Nominal concentrations of FC-120 were: 0 mg/L (control), 15, 25, 40, 60, and 100 mg/L. Nominal concentrations were used for all calculations. Daphnids used in the test were 'reduced from an in-house culture and were less than 24 hours old at the start of the test. After 48 hours of exposure the control daphnids had an average wet weight (blotted dry) of 0.0002 g, resulting in a loading rate of 0.005 g/L. All daphnids were in good condition at the beginning of the study. Exposure of daphnids to the test substance resulted in a 48 hour EC50 of 44 mg/L FC-120, with a 95% confidence interval of 25 to 60 mg/L. Six of the surviving daphnids exposed to 60 mg/L and all of the surviving daphnids exposed to 100 mg/L were immobilized at 48 hours. No other sublethal effects were observed in any test vessel. Page 2 of 14 000140 II. TABLE OF CONTENTS SECTION: I . Summary II. Table of Contents III. Index of Tables andFigures IV. Introduction V. Methods and Materials VI. Results VII. References Appendix A. Water Quality Data from Toxicity Test 4 PAGE 2 3 4 5 5 8 11 12 Page 3 of 14 000141 III. INDEX OF TABLES AND FIGURES Table 1. Chemical characterization of a representative sample of natural well water used as dilution water for toxicity test Table 2. Survival and sublethal effect data from toxicity teBt Table 3. Median effective concentrations (EC50s) from toxicity test Table A. 1. Conductivity, pH, temperature, and dissolved oxygen concentration measured during toxicity test PAGE 6 9 10 13 t Page 4 of 14 000142 IV. INTRODUCTION This study was sponsored by 3H Company, St. Paul, Minnesota. The objective of the study was to determine the acute toxicity of FC-120 to the daphnid, a freshwater invertebrate. The report contains sections that describe the methods and materials employed in the study, and the results of the investigation. The report also contains an appendix that presents the water quality data collected during the test. TEST SUBSTANCE: V. METHODS AND MATERIALS FC-120 (EnviroSystems Sample Number 5103E) was delivered to EnviroSystems on November 13, 1991. It was contained in a 250 ml plastic bottle that was labelled with the following information: "J2904-I, FC-120, Lot 164, 2-Butoxy Ethanol." FC-120 was supplied by 3M Company, 935 Bush Avenue, St. Paul, Minnesota. Prior to use the test material was stored in the dark at room temperature. A reserve sample will be retained at EnviroSystems for a minimum of 10 years. DILUTION WATER: Water used for acclimation of test organisms and for all toxicity testing was collected from wells at EnviroSystems in Hampton, New Hampshire. Water was stored in 500-gallon polyethylene tanks where it was aerated. Results of chemical analysis of a representative sample of water are presented in Table 1. TEST ORGANISM: Juvenile daphnids employed as test organisms were from a single source and were identified using an appropriate taxonomic key. Daphnids used in the test were produced from an in-house culture and were less than 24 hours old at the start of the test. Control daphnids were weighed at the conclusion of the toxicity test. Prior to testing, daphnids were maintained in 100% dilution water under static conditions in 4 liter glass jars. During acclimation daphnids were not treated for disease and they were free of apparent sickness, injuries, and abnormalities at the beginning of the test. During the acclimation period 24 hours prior to the test initiation the temperature was 20.3C. Daphnids were fed yeast, trout chow, and/or the freshwater alga Selenastrum caprzcornutum once or twice daily before the test. Page 5 of 14 000143 y * w Table 1. Chemical characterization of a representative sample of natural well water used as dilution water for toxicity test Parameter pH Conductivity Hardness Organochlorine pesticides Organophosphorus pesticides Polychlorinated biphenyls Unit of Measurement Reporting Limit pH units umhos/cm -- mg/L as CaCO^ -- ug/L 0.5 ug/L 2 ug/L 1 Measured Value* 7.2 540 166 ND ND ND Notes: 1. ND= not detected at or above the reporting limit. 2. pH, hardness, and conductivity were measured in dilution water collected from the control test vessel prior to introduction of organisms and pesticide and PCB data is collected during routine biannual water quality testing. * Page 6 of 14 000144 TOXICITY TESTING: A screening test with the test substance was conducted during January 21 to 23, 1992. Nominal concentrations of test substance were 1, 10, 100, and 1,000 mg/L. After 48 hours of exposure there was 0% survival at 100 and 1,000 mg/L and 80% survival or greater at all lower tested concentrations. A definitive toxicity test was attempted from January 29 to 31, 1992. Nominal concentrations of test substance were 150, 250, 400, 600, and 1,000 mg/L. This test was repeated because less than 50% survival occurred at all tested concentrations. The definitive toxicity test was performed during February 5 to 7, 1992. The test was conducted at a target temperature of 20 1C with five concentrations of test substance and a dilution water control. A 1,000 mg/L stock solution was made by combining 0.25 g of the test substance and dilution water in a 250 ml volumetric flask and adjusting the final volume to 250 mis with dilution water. Nominal concentrations of the test material were: 0 mg/L (control), 15, 25, 40, 60, and 100 mg/L. Twenty daphnids were equally distributed among four replicates of each treatment. The test was performed in 250 ml glass beakers that contained 200 ml of test solution (water depth was approximately 6 cm) . Test vessels were randomly arranged in an incubator during the 48 hour test (a random numbers table was used to select the location of each vessel). A 16 hour light and 8 hour dark photoperiod was automatically maintained with cool-white fluorescent lights that provided a light intensity of 20 uEs-1m~^. Aeration was not required to maintain dissolved oxygen concentrations above acceptable levels. Daphnids were not fed during the test. The number of surviving organisms and the occurrence of immobilization (inability to swim within 15 seconds after gentle agitation) or other sublethal effects (loss of equilibrium, erratic swimming, loss of reflex, excitability, discoloration, or change in behavior) were determined visually and recorded initially and after 24 and 48 hours. Dissolved oxygen (YSI Model 57 meter), pH (Beckman model pHI 12 meter), conductivity (Cole Parmer number 1481-60), and temperature (ASTM mercury thermometer) were measured and recorded daily in each test chamber that contained live animals. STATISTICAL METHODS: Results of the toxicity test were interpreted by standard statistical techniques (Stephan, 1983). The 24 and 48 hour EC50 values were calculated by the binomial, moving average, or probit method. All calculations were performed by the author using nominal concentrations of the test substance. Page 7 of 14 000145 pi- VI. RESULTS All test vessels containing PC-120 were clear and remained clear throughout the test. Biological and water quality data generated by the acute toxicity test are presented in Table 2 and Appendix A, respectively. One hundred percent survival occurred in the control exposure. Control daphnids had an average wet weight (blotted dry) of 0.0002 g at the end of the test. Loading rate during the toxicity test was approximately 0.005 g/L. Exposure of daphnids to the test substance resulted in a 48 hour EC50 of 44 mg/L FC-120, with a 95% confidence interval of 25 to 60 mg/L. Six of the surviving daphnids exposed to 60 mg/L and all of the surviving daphnids exposed to 100 mg/L were immobilized at 48 hours. No other sublethal effects were observed in any test vessel during the test. Page 8 of 14 000146 Table 2. Survival daba from toxicity test Nominal Concentrt ion (mg/L) rep. Number Alive 0 hr 24 hr 48 hr Number Affected Ohr 24hr 48hr 0 (control) 15 25 40 60 100 i 2 3 4 1 2 3 4 1 2 3 4 1 2 3 4 1 2 3 4 1 2 3 4 555 55 5 555 555 55 5 5 54 5 43 55 5 553 555 543 555 544 543 552 554 5 52 551 553 552 530 540 53 1 551 000 000 000 000 000 000 000 000 000 000 000 000 000 000 000 000 001 00 1 002 002 000 0 00 001 001 Note: Affected organism vere immobilized (unable to swim within 15 seconds after gentle agitation). 9 of 14 000147 &> # '9 'W 4M Table 3. Median effective concentrations (EC50s) from toxicity test Exposure period EC50 95 percent confidence limits Calculation method 24 hours 48 hours >100 mg/L 44 mg/L ---- 25 - 60 mg/L -- Binomial/Nonlinear Interpolat ion i i Page 10 of 14 000148 VII. REFERENCES OECD. 1984. Guidelines for Testing of Chemicals. Section 2: Effects on Biotic Systems. Method 202, Daphnid sp.. Acute Immobilization Test and Reproduction Test. Adopted April 4, 1984. Stephan, C.E. 1983. Computer Program for the Calculation of LC50 Values. U.S. EPA. Duluth, MN. Personal Communication. 1 Page 11 of 14 000149 Appendix A. WATER QUALITY DATA FROH TOXICITY TEST I i Page 12 of 14 0001S0 Table A.l. Conductivity, pH, temperature, and dissolved oxygen concentration measured during toxicity test Nominal ' concentration (mg/L) Rep. Conductivity (umho/cm) pH ----------------------- ------------------ 0 24 48 0 24 hr hr hr hr hr 0 (control) 15 25 40 60 100 1 2 3 4 1 2 3 4 1 2 3 4 1 2 3 4 1 2 3 4 1 2 3 4 540 550 570 570 580 580 580 580 580 580 580 580 580 580 580 580 580 580 580 580 580 580 580 580 580 600 580 590 580 590 580 580 580 590 570 580 570 580 570 590 580 590 580 580 580 580 580 580 580 590 580 580 580 580 580 590 580 590 580 590 570 580 580 < 590 590 590 580 580 580 580 580 590 7.2 8.0 7.2 8.1 7.2 8.1 7.2 8.1 7.2 8.1 7.2 8.1 7.2 8.1 7.2 8.1 7.2 8.1 7.2 8.1 7.2 8.1 7.2 8.1 7.2 8.2 7.2 8.2 7.2 8.2 7.2 8.2 7.2 8.2 7.2 8.2 7.2 c .2 7.2 9.2 7.2 8.2 7.2 8.2 7.2 8.2 7.2 8.2 48 hr 8.0 8.0 8.0 8.0 8.0 8.0 8.0 8.0 8.0 8.0 8.0 8.0 8.1 8.1 8.1 8.1 8.1 8.1 8.1 8.1 8.1 8.1 8.1 8.1 Page 13 of 14 000151 Table A.1. Continued Nominal concentration (mg/L) Rep. Temperature (C) ----------------------- 0 24 43 hr hr hr 0 (control) 15 25 40 60 100 1 2 3 4 1 2 3 4 1 2 3 4 1 2 3 4 1 2 3 4 1 2 3 4 19.4 19.4 19.4 19.4 19.4 19.4 19.4 19.4 19.4 19.4 19.4 19.5 19.4 19.4 19.6 19.4 19.4 19.4 19.4 19.4 19.4 19.4 19.4 19.4 20.5 20.3 20.3 20.2 20.3 20.3 20.2 20.3 20.3 20.3 20.3 20.2 20.6 20.6 20.4 20.3 20.8 20.8 20.6 20.4 20.8 20.8 * 20.8 20.7 20.4 20.3 20.3 20.4 20.3 20.3 20.2 20.4 20.4 20.3 20.3 20.3 20.3 20.3 20.4 20.4 20.4 20.4 20.4 20.4 20.4 20.4 20.4 20.4 Dissolved Oxygen (mg/L) ----------------------- 0 24 48 hr hr hr 8.7 8.9 9.3 8.8 9.0 9.2 8.8 9.1 9.3 8.8 9.0 9.2 8.9 9.0 9.0 9.0 9.0 9.0 9.0 9.0 9.0 9.0 9.0 9.1 9.0 9.0 9.1 8.9 9.0 9.1 8.9 9.0 9.1 9.0 8.9 9.1 8.9 9.0 9.1 8.9 8.9 9.1 8.9 8.8 9.1 8.9 8.8 9.1 8.9 9.0 9.1 9.0 8.8 9.1 9.0 8.9 9.1 8.8 8.8 9.1 9.0 9.0 9.1 9.0 9.0 9.1 9.0 8.8 9.1 8.9 8.8 9.1 Page 14 of 14 000152