Document VKEkO5x9Ze1KVeq2D5YMqkm5p
M'v. DU ?<# USE
AR226-2950
Du Pont HLR 349-90
Study T it le
STATIC ACUTE 48-HOR EC50 0F|
fiO DAPHNIA MAGNA
Data Requirement United State,,s Environmental Protection Agency
Environtentai Effects Test Guidelines Subdivision EG-1
Author
Kevin N. Baer, Ph.D.
!...
!study Completed On
I 16 July 1990 I
Performing Laboratory
I .. E. I. du Pont de Nemours and Company, Inc. Haskell Laboratory for Toxicology and Industrial Medicine
ElktonjRoad. Post Office Box 50 Nvark, Delaware 19714
Laboratory Project ID Haskell laboratory Report No. 349-90
Page 1 of 13
Company Sanitized. Does not contain TSC A CBI
#
iVMV*L-'}'``QUALI!r ASSURANCPQGU^NTTION
.UDY: H# 18,267
STATIC ACUTE 48-HOUR EC50 OFl
AUDITS: Items Audited Protocol, Conduct Records, Report
Audit Dates 5/24/90 7/5-6/90
fTO DAPHNIA MAGNA
SHORT-TERM AUDIT REPORT NUMBER: DATE FINDINGS REPORTED TO MANAGEMENT AND STUDY DIRECTOR:. 7/6/90
; ..
Reported by
j William J. Lynam
Quality Assurance Auditor
I
Date
- .3 -
L Does not contain TSC A CBI
Good Laboratory Practice Statement
Quality Assurance Documentsfcion
Table of Contents
I. Purpose
II. Introduction/Summary .!......... ..................... |
Records General Information -
III. Materials and Methods
A. Test Material . . B. Dilution Vater . C. Animal Culture . D. Test Methods . . E. Chemical Analysis
7
7 7 7 7
8
IV. Results and Discussion
8
V. Conclusion . . . . . .
8
VI. Literature Cited . . .
8
VII. Tables.......... I.............**: Table 1. Chemical Characteristics of Haskell Laboratory Veil
Vater...... ........ . .............*
Table 2. Vater Chemistify of the Vater Contro^a^est Start
Table 3. Dissolve^xyj en Concentration of
....
Table 4. pH of
....... * ' ' ` * * ` *.* ' ` '
Table 5. Observed Immobility of Daphnja magna Exposed to
for 48 Hours in a Static, Unaerated Test .
9
9
10 11 12
13
i
I)
> ; i........ -
4 C o m p a n y anJzed. u o e s izt c o n ta in T S C A CBS
------------------------------------ --
24
^exhi hours old)
bi in
atneundaerhaot,*1cu3,t2e0s0t7attoWixcidciit5'y0h0t0ooumrg/#ts.t. TahNmeoamg4in8na-ahlnoeuocrnoanBtcCe5es0ntrc(aoltueilsosdnsntohtan
wbeerecal1c31u1late1d638beca20u4s8e50205060i|g/L, the hipest concentration tested, failed to
cause immobilization of the daphnids.
RECORDS
aLracbhoirvaAetldolryadtatfaotrhaenTDdoxuitcPhooelnotfgiyRneaealnodrredpsIonrMdtaunswatigrleilmaelnbtMeedCmieacnitinentreain((eNWdeiwlaemriiknt,ghteorDne,altawDaHerlaeas)wkaerloelr).be
Technician: OA-UaM * - j-x ! / L / / Charles L. Hall Environmental Toxicology and
Information Services
7 d
JQ
Day/Ho/Year
Study Director:
Research Toxicologist Environmental Toxicology and Information Services
2~i Jmc 9O Day/Mo/Year
THIS REPORT IS APPROVER ISSUE.
.A/. 0S.x. RiVin N.iBaer, Ph.D. Study Director
H. Slu ?Q Day/Mo/Year
Company Sanitised. Does not contain TSCA CB!
Haskell Number: Synonyms: Purity: Composition:
Sponsor: Submitted by: 'Study Schedule: Notebooks:
I! Chemicals and Pigments Department E. I. du Pont de Nemours and Co.Inc. I Jackson Lab
I Chemicals and Pigjnents Department ! E. I. du Porit de'Nemours and Co. Inc.
Jackson Lab
Study Initiation: ,21 May 1990 ! Experimental Start: 24 May 1990 I Experimental Termination: 26 May 1990 I Study Completion: 16 July 1990
Haskell Laboratory Cost Code: i
Distribution:
j
6
CG'PaOY
I
Du Pont HLR 349-90
i n . ! MATERIALS and methods
A. Test Material
j
i. . . . t
Source, composition wdjpurity of
'_ are described under General ^ ^ concentration was
ir.1
sr >"d
~ e aarmla8.
B. Dilution Water
in these daphnid tests.
C. Aimal Culture
beakerPsare(n2t1 ^PaTpbhiniikaemra)gnwahwiecrhecorne*t*a?i*nJed ^1.g8^Loof ftiilltteerreead^iluteion# wmaitxetrurheelodf
at approximately 20C.
chJJ a*d Fieischmann's yeast three
frozen Purina (same as Buckshire) *
Neonates were collected from
times weekly during the water renewal PJ!" 8! : ^ 60" ^ * ^ , injury or
15 day old parent daplm!d* for "?* *" identified by labels on the beakers abnormality was not present. Animals were loentine *
or test vessels.
-
D. Test Methods [1] Six test concentrations and a water control we^ . 2 0 4 8 f 2560,
fish tal* " tar. Nominal last concantrations .era 1311, 1638, 2048, 250 , 3200 and 5000 mg/L.
Pyre* baakars (250 .hi 6.1-am ^ ' " ^ I f ^ r ^ a / c o S c ^ r a . i a n )
solution (6.8-cm depth) were used. contained five daphnids (<0.1 g
^Danhnids were added to the water lowest concentration. Random
control and to test beakers be?;nn*"?
^test solutions of low
assignment was not used to avoid co
Hhnids vere released under the
concfntration from the transfer jig*, jtan
/ " Solutions were
water surface. Daphnids ? fJ0 06C) Jnd vere unaerated. A photoperiod
T l S bZ l t \ l l " 06 ?; 592(S versus}8 hours darkness was employed.
7 tSCA^
Du Pont BLR 349-90
D1ssolved oxygen,m&
,ter controls and in all
replicates of every fei%:cdni
jnids vere added at the
J.T-
beginning end at the
conductivity of the dilution^ Temperatures vere taken yith |n iteeri!c^'Sdnij,
iity, EDTA hardness end the beginning of the test. Testing Materials
calibrated mercury thermometer
:!
il B. Chemical Analysis
fe No chemical analyses of the test splufiohs! Vere performed.
m
IV RESULTS AND DISCUSSION
An earlier rangefinding sjtudy vas conducted but the results vili not be reported in this study.
Tr;7ts solutions of ( l exhibited a milky white color. All chemical
nJFi ''ji j ..-"
and physical parameters (Tables 2, 3 and 4) were within expected ranges. The 48-Hr BC50 could not be calculated because 5000;mg/L, the highest
concentration tested failed to cause immobilisation of the daphnia (Table 5).
iv<"'
V. CONCLUSION
,
IS.
exhibited no acu.te toxicity in this static, unaerated 48-hour Daphnfa magna test. The EC50 vas greater than 5000 mg/L, the highest
concentration tested.
VC. LITERATURE CITED
1. United States Envirinmental Protection Agency, Office of Toxic Substances, Guidelines for Testing Chemicals, EG-1, Washington, DC., August 1982.
4 4
V Company Sanitized. Does not contain T S C A CBJ
MB
Du Pont BLR 349-90
TABLE 1
Chemical Character!sties of Haskell Laboratory Well Water
Parameter
i
Concentration
Parameter
Concentration
BOD, mg/L COD, mg/L
DOC, mg/L TOC2*, mg/L Kjeldahl N, mg/L Ammonia N, mg/L Organic N, mg/L
T-rbidity, NTU
Phenolics, mg/L
5j <10
<1,0
0.8 <0l2
<012 <0,2
< 1,0 <0^05
Color, Co/Pt
<5
Solids
total suspended, mg/L <4 :
Aluminum, mg/L
<0^100
Antimony, mg/L
<0,060
Arsenic, mg/L
<0 . 0 1 0
Beryllium, mg/L
<01001
Boron, mg/L
<0,021
Cadmium, mg/L
<0,002
Calcium, mg/L
25,4
Chloride3, mg/L
6.3
Chromium, mg/L
<0.010
Cobalt, mg/L
<0.020
Copper, mg/L
<0;010
Cyanide, mg/L
<0,025
Iron3, mg/L
0.131
Fluoride3, mg/L Lead, mg/L Magnesium, mg L MBAS/US, mg/L
Mercury, mg/L Nickel, mg/L Nitrite3, mg/L
Nitrate3, mg/L Phosphate3, mg/L
Potassium, mg/L
Selenium, mg/L Silver, mg/L Sodium, mg/L Sulfate3, mg/L Sulfide, mg/L
Zinc, mg/L Volatile priority pollutants Acid extractable priority pollutants
Base/neutral priority pollutants Pesticides/PCBs
Organophosphate pesticides4, ug/L
<0.1 <0.075
3.9 <0.05 <0.00020 <0.020
<0 . 1
1.7 <0.1 1.8 <0.005
<0.010 8.1
5.0 0.11 <0.020
ND5
ND6
ND6 ND
<0.50
Date of analyses 24 October 1989 unless indicated othervise.
2Date of analyses 5 December 1989. 3Date of analyses 11 December 1989. 4Date of analyses 26 September 1989. None detected except methylene chloride was below the published Method Detection Limit (BMDL). None detected.
I 1
10 not contain TSCACBl
D CO'T'P3^ san**1-0085
1Corning Model 610A, Caning Scientific Instruments, Hedfield, MA.
j
2Each replicate contained 5 daphnids.
12
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Du Pont BI& 349>9B
TABLB 5 Observed Immobil!1v; of Daohhla magna Imposed *o
for 48 Bouirs in Htic^naerted Test
Nominal Concentration
(mg/L)
Percent Cumulative Observed Immobility
i!4 Hours A*1 B* C1 D1
48 Hours A1 B* Ci Dl
Vater Control 0 ! o 0 l
1311 0 j 0 0 0
1638 2048
0 1! 0 0 0 1
0 li o 0 0
2560 0 0 0 0
3200
0000
5000
0 00 0
__________ ______ -i----------- -- I
1Each replicate contained five daphnids*
0 000 0 000 0 000 0 000 0 000 0 000 0 000
i
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tscacbi Sanrted.Does not contain
ComP30*
