Document VGrv54BN1MRJL7Bn4wa5qdOaq

AR226-2965 DU PONT USE ONLY Du Pont HLR 636-91 Study Title STATIC, ACUTE, 96-BOUR LC50 TO FATHEAD MINNOWS (PIMEPHALEs M W A b ) Author Kevin N. Baer, Ph.D. Study Completed On September 24, 1991 Performing Laboratory E . I. du Pont de Nemours and Company Haskell Laboratory for Toxicology and Industrial Medicine Elkton Road, Post Office Box 50 Newark, Delaware 19714 Laboratory Project ID Haskell Laboratory Report No. 636-91 Page 1 of 12 CompanySanRfeed.Doesnoicontain rs n A CBt Du Font ILE 636-91 TABLE OF CONTENTS Page ; Table of Contents . . . . . . ................. . . . . . . ......... 2 I. S u m m a r y ................ 3 II. General I n f o r m a t i o n ........................... 4 III. P u r p o s e ............ 6 IV. R e c o r d s .......... 6 V. Materials and M e t h o d s ................... ................ .. 7 A. Test M a t e r i a l s ................ 7 B. Dilution V a t e r .................. 7 C. Animal C u l t u r e .................. 7 D. Test M e t h o d s ................................................... 7 E. Chemical A n a l y s i s .............. 8 F. Statistical Methods . . . .................................... 8 VI. Results and D i s c u s s i o n ............ 8 J) VII. Conclusion ........................................................8 VIII. Literature Cited..................................................... 8 IX. T a b l e s ............................................................. 9 1. Chemical Characteristics of Haskell Laboratory Veil Vater . . 9 2. Vater Chemistry of the Dilution Vater at Test Start...........10 3. Physical and Chemical Characteristics o f j ^ B H t t A ^ e s t Solutions..................... 11 4. Observed Mortality j>f Fathead Minnows (Pimephales promelas) Exposed t o ^ m ( K o r 96 Hours in a Static, Unaerated Test . 12 KUfySsftRizscL Dess not contain YSC C* & 2 Du Pont HLR 636-91 I. SUMMARY -- ----- ^ moderately acutely toxic to juvenile, fathead minnows (Pimephales promelas? in a 96-hour, unaerated, static test using nominal test concentrations of 6.3, 13, 25, 50, and 100 og/L. The 96-hour LC50 was 21 mg/L with a 952 confidence interval of 16 to 29 mg/L. Ho/Day/Year Study Director: A / . t^ Kevin N. Baer, Ph.D. Research Toxicologist Environmental Sciences THIS REPORT IS APPROVED FOR ISSUE. Study Director y/ W <?/ Mo/Day/Year f/y fa / Mo/Day/Year 3 tmmm&a? ix. g e n e r a l im m m m m Du Font HLR 636-91 Material Testais Medical Research Numbers Haskell Numbers 18,920 Haskell Laboratory Test Code: )) Sponsors Du Pont Chemicals E. I. du Font de Nemours and Company Wilmington, Delaware 19898 Company Sanitizes!.Doesno!containTSG CBf Submitted Byi Da Pont HLR 636-91 1 Jackson Laboratory Deepwater, NJ Study Schedule: Study Initiation Date: 8/30/91 Experimental Start Date: 9/5/91 Experimental Termination Date: 9/9/91 Study Completion Date: 9/24/91 ' Distribution H ie s not contain YSC CBI 5 -- ~ s w . * . ' . . - ; -------- --------- Du Pont HLR 636-91 III. PURPOSE Hie goal of this study as to assess the acute toxicity of juvenile, fathead minnows (finephales promelas). to IV. RECORDS All data and the final report vili be maintained either at Haskell Laboratory for Toxicology and Industrial Medicine (Newark, Delaware) or be archived at the Du Pont Records Management Center (Vilmington, Delaware). 6 Du Font HLR 636-91 V. MATERIALS AND METHODS A. Test Materials Source and composition of the test substance are described under General Information. The appropriate quantity measured and dispensed into 4-L of dilution water (seet^low) to'ptepare each test concentration. Stability and solubility o f B H H i n dilution water were not determined. B. Dilution Water Water (Table 1) originated from the Haskell Laboratory well. C. Animal Culture Fathead minnows (Pimephales promelas) were reared in continuously flowing well water at Haskell Laboratory. Adult fish reside in 38 L aquaria at approximately 25C and were fed frozen brine shrimp (Artemia S., Golden Gate Brand, Korden Division, Novalek, Inc., Hayward, CA) generally twice daily, once on Saturday, and not on Sundays. Eggs were deposited on polyvinyl chloride tiles coated with sand. Tiles with eggs were aerated and incubated at approximately 25 C in a water bath. Larvae were reared in 22 L aquaria at approximately 22"C and were fed newly hatched Artemia generally twice daily on weekdays and daily on weekends. Juveniles were held in a 22 L aquaria at approximately 22C and were fed newly hatched Artemia and/or frozen brine shrimp generally twice daily on weekdays and once on Saturday and Sundays. No treatment of the fish for diseases was required during the holding period. Sickness, injury, and abnormality were not observed. Animals were identified by labels on aquaria and test vessels. D. Test Methods [1) Five test concentrations and a water control were used. Nominal concentrations were 6.3, 13, 25, 50, and 100 mg/L. The fathead minnows ranged from 1.3 to 2.0 cm in standard length (mean 1.5 cm) and 0.024 to 0.096 g in wet weight (mean 0.044 g). The fathead minnows -e 51 days old at test start. Glass beakers (4+-L) containing 4-L of test solution (approximately 13-cm liquid depth) were employed. One replicate of each test concentration with ten fish each were used. Fish were added to the test concentrations using random numbers and were not fed for 48 hours prior to the test nor during the test. Mean loading for controls at test conclusion was 0.11 g/L. Test solutions were held between 21.0 and 21.8C (mean 21.4C) and were unaerated. A photoperiod of 16 hours light (129-183 Lux) versus 8 hours darkness was employed. Dissolved oxygen (YSI Model 54A, Yellow Springs Instruments, Yellow Springs, OH) and pH (Corning Model 610A, Corning Scientific Instruments, 7 Company Sanitized.Does notcontainTSC CB "'u Pont BLR 636-91 Kedfield, HA) were measured in the control and all test concentrations at the of the test (before the addition of fisih) and daily thereafter. Temperature (American Society of Testing Materials registered mercury stem thermometer) was measured in the dilution water control at the beginning of the test and daily thereafter. Total alkalinity (Hach kit, Hach Chemical Company, Loveland, CO), EDTA hardness (Hach kit, Hach Chemical Company, Loveland, CO), and conductivity (YSI Model 33, Yellow Springs Instruments, Yellow Springs, OH) of the dilution water were measured at test start. E. Chemical Analysis conducted. analysis of the test substance in the test solutions was not F. Statistical Methods The 96-hour LC50 and its associated confidence interval was calculated using a moving average-angle program [2]. VI. RESULTS AMD DISCUSSION N o m i n a l & H B H A concentrations used in the definitive test were 6.3, 13, 25, 50, and 100 mg/L. All chemical and physical parameters were within expected ranges (Table 2 & 3). The 96-hour LC50 was 21 mg/L with a 95% confidence interval of 16 to 29 mg/L. VII. CONCLUSION JM w a s moderately acutely toxic to juvenile, fathead minnows (Pimephale^promelas) in a 96-hour, unaerated, static test using nominal test concentrations of 6.3, 13, 25, 50, and 100 mg/L. VIII. LITERATURE CITED 1. Zucker, E. 1985. Hazard Evaluation Division, Standard Evaluation Procedure: Acute Toxicity Test for Freshwater Fish. EPA-54019-85-006. United States Environmental Protection Agency, Office of Pesticide Programs, Washington, DC 20460. 2. Peltier, W.H. and C.I. Weber, Eds. 1985. Methods for Measuring the Acute Toxicity of Effluents to Freshwater and Marine Organisms. EPA/600/4-85/013. United States Environmental Protection Agency, Environmental Monitoring and Support Laboratory, Cincinnati, OH. L.*"" 8 noie-enlaiiTSCA r Du Pont HLR 636-91 TABLE 1 rhararteriatics of Haskell.Laboratory Wel1 Vaterl Parameter Concentration Parameter Concentration BOD2 , rag/L wl/ nsgr1 DOC3, mg/L TOC4, mg/L Kjeldahl N, mg/L Ammonia N, mg/L Turbidity, NTU Phenolics, mg/L Color, Co/Pt <2 13 1.5 <0.5 0.2 <0.05 <1.0 <0.050 <5 Solids total suspended, Aluminum, mg/L mg/L <2 <0.100 Antimony, mg/L Arsenic, mg/L Beryllium, mg/L Boron5-, mg/L Cadmium, mg/L <0.060 <0.010 <0.001 <0.020 <0.002 Calcium, mg/L 25.0 Chloride, mg/L Chromium, mg/L Cobalt, mg/L Copper, mg/L 6.3 <0.010 <0.020 <0.010 Cyanide, mg/L <0.025 Iron, mg/L Fluoride, mg/L 0.053 <0 . 1 Lead5 , mg/L Magnesium, mg/L MBAS/LAS, mg/L Hercury, mg/L Nickel, mg/L Nitrite, mg/L Nitrate, mg/L Phosphate, mg/L Potassium, mg/L Selenium, mg/L Silver, mg/L Sodium, mg/L Sulfate, mg/L Sulfide, mg/L Thallium, mg/L Zinc5, mg/L Volatile priority pollutants Acid extractable priority pollutants Base/neutral priority pollutants Pesticides/PCBs Organophosphate pesticides7, yg/L <0.005 3.5 <0.05 <0.00020 <0.020 <0.1 1.6 <0.1 1.1 <0.005 <0.010 8.1 4.8 <0.05 <0.01 <0.020 ND* ND8 ND8 ND8 <0.50 _, .. ..,,ho, inno unless indicated otherwise, analyses L " ." m e n t i o n Corporation. Mi,on. He. performed at Environment analyses analysis performed at Jersey} 2Date Environmental of Testing and ratification Certifica Corporation, P mEdiPsoonnt, New Jersey} Mean Engineering Test vaine, -Date ot J2e P u b ? S 1 e o d DeteetloS Liai, (BHDL), S ; r ; e ^ r ;b.r S ^ i S S S ' . F S S S i LaboratoriU. Ino., Hadl.on. I, -Hone detected. I l 9 Du Pont HLR 636-91 TABLE 2 Vater Chemistry of the Dilution Vater at Test Start Total Alkalinity (ffig/L as CaC03) 81 EDTA Hardness (mg/L as CaC03> 74 Conductivity (yrahos/cm) 190 10 I Du Pout HLR 636-91 TABLE 3 Physical and Chenical Characteristics of Test Solutions Time (Hours) Control Nominal Concentrations (mg/L) 6.3 13 25 50 100 0 8.7 8.7 8.7 8.7 8.6 8.6 24 6.2 6.2 6.5 6.4 6.8 7.0 48 6*0 6.1 6.4 6.2 6.5 6.9 72 6.1 6.3 6.2 6.4 6.4 6.5 96 6.7 6.3 6.2 5.9 6.0 6.3 Dissolved Oxygen 0 24 48 72 96 7.4 7.2 7.3 7.2 7.9 7.4 7.5 7.5 7.5 7.1 7.5 7.5 7.5 7.2 7.4 7.4 7.4 7.2 7.3 7.2 7.2 7.9 7.9 7.9 7.9 7.5 7.5 7.4 7.2 7.9 ippmpany Observed Ho: to, Du Pont BUR 636-91 TABLE 4 Fathead Minnows (Pimephales promelas) Exposed :or 96 Honrs in Static. Unaerated Test Nominal Concentrations (mg/L) Control 6.3 13 25 50 100 Observed Mortality in Percent Time (Hours) 24 Hours 0 0 0 0" 20" 60b 48 Hours 0 0 0 70c 100 100 72 Hours 0 0 0 80 100 100 96 Hours 0 0 0 80" 100 100 Five fish were at the surface, were dark in coloration, and had an accumulation of an unknown substance on their pectoral fins. Four fish were at the surface, were dark in coloration, and had an accumulation of an unknown substance on their pectoral fins. cAll surviving fish were at the surface and had a dark coloration. All ,,surviving fish exhibited dark coloration. mJit sS Q A Cfel 12