Document VGNmp9DQJn980Exp6xngxK7DN
ACUTE TOXICITY TO FISH (FATHEAD MINNOW) TEST SUBSTANCE
Identity:A mixturecontainingperfluorooctanesulfonatmea;y also be referredtoas PFOS, FC-95, or as a component of FC-206CF or FC-206CF-X. (1-Octanesulfoniaccid)(CAS # 2795-39-3).
Remarks: The 3M productionlotnumber was 101. The testsample isFC206CF. Currentinformationindicatesitisa mixtureof0.5% PFOS, 10% diethyleneglycolmonobutyl ether,78.08% water,1.375% acrylicfoamer, 1.5% sodium octylsulfate1,% sulfonatedalkylethersalts,6.0% urea,0.5% trietahnolamine, and 0.05% tolyltriazole.
The following summaty applies to a mixture with incompletely characterized concentrations of impurities. Data may not accurately reflectthe toxicityof the fluorochemical component of the testsample.
METHOD:
Method: OECD Guideline203. Type: Acute static GLP: No
Year completed: 1991 Species: Pimephalespromelas
Supplier:AquaticResearch Organisms,DivisioonfResource Analysts,Inc., Hampton, NH.
Analyticalmonitoring: Temperature, pH, conductivitya,nd dissolved oxygen.
Exposure period: 96-hours
Statisticalmethods: No calculationpserformed, based on study results. Test fishage: Juveniles
Length and weight:
Average length 34 mm
Loading: 0.15g/L
Average weight 0.23g (wet)
Pretreatment:None
Test conditions:
Dilutionwater: Filterendaturawlellwater
Dilutionwater chemistry:
Hardness:
80 mg/L as CaC03
Conductivity: 830 pmhos/cm
pH:
7.7
Lighting: Cool-white fluorescentlights.A dailyphotoperiod of 16 hours light and 8 hours dark was maintainedthroughoutthe testingperiod.
Stock and testsolutionpreparation: The testmaterialwas added directly to the dilutiownater on a weight/volumebasis. Concentrations dosing rate: Once.
StabiliotfythetestchemicalsolutionsN:otnoted. Exposurevessels:20 L glassaquari(a40x2Ox25cm) containin1g5 L oftest solutionand an approximatewater depth of 18 cm. Number of replicates:2 Number of fishper replicate:10. Number of concentrations: fiveplusa blankcontrol Water chemistry during the study:
pH range (0-96 hours): 7.1- 7.7 (controelxposure) 7.6- 7.8(1000 mg/L exposure)
Temperature range (0-96hours): 21.7- 22.7 OC Conductivityrange (0-96hours):
830 - 850 pmhos/cm (controelxposure) 840 - 870 pmhos/cm (1000 mg/L exposure) Dissolved oxygen range (0-96hours): 6.8 - 8.1 mg/L (controelxposure) 5.2 - 8.5 mg/L (1000 mg/L exposure)
RESULTS
Nominal concentrations: Blank control,150,250, 400, 600, and 1000 mg/L
Element values: 96-hour LC50 = >1 000 mg/L
Element values based on nominal concentrations
Remarks: Testingwas conducted on the mixtureas describedinthe Test Substance Remarks field.The values reportedapply tothatmixtureand not the fluorochemicalproportionalone.
CONCLUSIONS
The testsample 96-hour LC50 forfathead minnow was determined to be >1000 mg/L.
Submifter: 3M Company, Environmental Laboratory,P.O. Box 33331, St. Paul,Minnesota,55133
DATA QUALITY
Reliability:Klimischranking= 2. Testingmeets the criterifaorquality testing.However, the sample puritywas not propedy characterizedand the study lacksanalyticaclonfirmationofthe amount offiuorochemicalproportion inthe solution.
REFERENCE
This testwas conducted by EnviroSystems DivisionR,esource Analysts, Incorporatedo,fHampton, NH atthe requestofthe 3M Company, St.Paul, MN, Lab Request number J1884-4, 1991.
OTHER
Last changed: 6/28/00
amay Ti-tie Static Acute Toxicity of TC 206cr to the
Fathead Minnow, Piseph&les pro,,elas
Authors Tinothy J. Vard Robert L. Boeri
Study Completed June, 1991
PerfornizigLaborato EnviroSystess Division,,'@, Resourco'knalYStB,, Incoroorated:-,.,-,,,
One Lafayette R asptOni NOT Rupshire -@;'03842
@'A
The acute toxicity of FC 206CF to the fathead minnow, Pinephales
promelas, is described in this final report. The test was conducted
for 3M Company for 96 hours during June 3 to 7, 1991, at the EnviroSystess Division of Resource Analysts, Inc. in Hampton, Kew Hampshire. It was conducted by Jeanne Nagazu, Peter Kowalski, Ellen Stanford, Robert Boeri, and Timothy Ward
The test was performed under static conditions with five concentrations of test substance and a dilution water control at a mean temperature of 22 IOC. The dilution water was filtered natural well water collected at Hampton, New Hampshire. Aeration was not employed to maintain dissolved oxygen concentrations above an acceptable level. Nominal concentrations of IPC 206CY were: 0 no/L (control), 150 mg/L, 250 mg/L, 400 mg/L, 600 al/L, and 1,000 mg/L. Nominal concentrations were used for all calculations.
Fish used in the test were purchasedfrom a commercial supplier
(Aquatic Research Organisms, Hampton, New Hampshire) and acclimated
under test conditions for more than 15 days. After 96 hours of exposure the control fish had an average.totallength of 34 an and an
average wet weight (blotteddr7) of 0.23 g, resulting in a loading rate
of approximately 0.15 g/L. beginning of the study.
All fish were in good condition at the
brposure of fatbead minnows to the test substance resulted in a 96 hour, LC50 greater than 1,000 ag/L TC 206CIP. No sublethal effects were observed in any test vessel during the 96 hour exposure.
a';o-2-of-1-4
@7
NayftW-&bdcloydWIPORE
Ir. IABLZ Of CONTENTS
SECTION: PAGE
SumLmary 2
IT. Table of Contents 3
III. Index of Tables 4
IV. Introduction 5
V.
Methods and Materials
5
Vi. Results 8
VII. References
Appendix A. Water Quality Data from Toxicity Test 12
ag@i'-3 o f
A-ly" w- &tofty d
111. RNDIX Or TJLBLZS
Table 1.
Chenical ch!@,-acterizatioonf a representative sample of natural well water used as dilution water for toxicity test
Table 2. Survival data from toxicity test
Table 3.
Median lethal concentrations (LC509) from toxicitytest
Table A.I. Conductivity, pH, temperature, and dissolved oxygen concentration measured during toxicity test
PAGE 6
9 10 13
'7
IV. IPTRODucTrox
This study was sponsored by 3H Company,St. Paul, Minnesota. The objective of the study was to determinethe acute toxicityof TC 206CY to the fatboad minnow,a frtsbvaterfish. The reportcontainssectionsthat describe the methods and materials employedin the study, and the results of the investigation.The reportalso containsan appendixthat presents the water qualitydata collectedduring the test.
TEST SUBSTANCE:
V. IMTHODS AND MATERIALS
FC 206CF (EnviroSystessSample Number 2938E) was deliveredto EnviroSystess on May 23, 1991. It was containedin a 4 oz plasticbottle that was labelledwith the followinginformation:"Jl$84-4,FC206CF". FC 206CF (a light brown liquid) was suppliedby 3M Company, 935 Bush Avenue, St. Paul, Minnesota. Prior to use the testmaterialwas stored in the dark at room temperature.A reserve sample (approximately1.0 grams) will be retained at EnviroSystessfor a minimum of 10 years.
DILUTION WATER:
Water used for acclimationof test organismsan-ifor all toxicity testing was collected from wells at InviroSystessin Hampton, New Hampshire. Water was stored in 500-gallonpolyethylenetanks, where it was aerated. Results of chemical analysis of a representativesample of water.are presented in Table 1.
TEST ORGANISM:
Juvenile fatboad minnows employed as test organisms were from a single source and were,.identified,u:sing,,anappropriatetaxono c key. They were purchased'from.-a comaerciall,stipplie(rtheAquatic ;eisearch;@ organisms Division of Resource lnalysts Ijidw,gampton,,New ffaapxhire)-and acclimated at the EnviroSysteasfacilityfor more than 15 days. Control fish were weighed,at .the ..conclusion.of.,,,.tthoexicityt-est..Prior. testing, fish were" saintained@.@i1n00% dilutionwater under flow through* conditionsin an all glass',a@4uarium..@.D:u'ring acclimation, fish were not.,@,@ treated for disease and they.wero-'friosf;a@parentsickness, injuries@,and abnormalities at the beginning'bfthe.test.@--.*.Durintghe acclimation period 14 days priorto the test initiationthe to pteraturerangedfrom 21.80 to 23.6*C, and the dissolved oxygen.-'conceantriaon was, maintained-ab,)ve 7.6 mg/L. Fish were fed a commercial fish,food (EnviroSystemslot number Tnol) once or twiredaily before.thetest.
Page $'of 14'
Table 1. Chemical characterization of a representative sample of natural well water used as dilution water for toxicity test
Parameter
PH
Conductivity
Hardness
Organochlorine pesticides
Organophosphorus pestici,les
Polychlorinated bipbenyls
Unit of Measurement
Reporting Limit
PH units
unhos/cm
mg/L as CaCO3
--
ug/L
0.5
ug/L
0.5
ug/L
0.5
Measured Value 7.7 830 80 ND
ND
ND
Notes:
1. ND not detected at or above the reporting limit. pH, hardness, and conductivity were'seasured in dilution water collected from the control.test vessel prior to introduction'oforganisms and pesticide and PCB data is collected during routine biannual water quality testing.
ra w,a
lumLcohwlpkw-SAWdbydMLLPORE
TOXICITY TESTING:
A screening test with the test substance was not conducted and
historic data was used to choose the test concentrations. The definitive toxicity test was performed during June 3 to 7, 1991. it is
based on procedures of the OECD (1984). The test was conducted at a
target temperatureof 22 :k 10C with five concentrationsof test substance and a dilution water control. No stock solutionwas prepared
and test materialwas added directlyto dilutionwater containedin the
test vessels without the use of a solvent. Nominalconcentrationsof
the test materialwere: 0 mg/L (control),150 mg/L, 400 mg/L, 600 mg/L, and 1,000 mg/L.
250 mg/L,
Twenty fish were randomly and equally distributedamong two replicates of each treatment. The test was performedin 20 liter glass aquaria (approximately20 ca in width, 40 co in length,and 25 ca in height) that contained 15 liters of testsolution(waterdepth was approximately18 ca). Test vessels were randomly arrangedin an incubator during the 96 hour test (a random numbers table was used to select the location of each vessel). A 16 hour lightand 8 hour dark pbotoperiod was automatically maintained with cool-wbitefluorescent lights. Aeration was not employed to maintain dissolved oxygen concentrationsabove acceptable levels. risb were not fed during the test.
The number of surviving organisms and the occurrence of sublethaleffects (loss of equilibrium,erratic swimming, loss of reflex, excitability,d,iscoloration,or change in behavior) were determined visually,andrecordedinitiallyand after 24, 48, 72, and 96 hours. Dissolvedoxygen (TSINodal 57 meter; instrument number PRL-3), pE (Beckman model pEl@12 meter; instrument number PRL-51), conductivity (Labcoup SCT meter,.'.instrumentnumber PRL-3). and temperature (ASTM mercury thermometer;' thermometer number 1686) were measured and recorded daily in eact@test chamber that contained live animals.
STATISTICAL IM ODS:,. Results of @tbe toxicity test could not be interpreted by
standard statisticaltochniques@, (Stephan,i 1983) because greater than 50% survivaloccurred in all testedconcentrations.
age 7'of 14,1.
vi rl!SMTS
All test vessels containing PC 206CT were initially clear and remained clear throughout the test. Biological and water quality data
generated by the acute toxicity test are presentedin Table 2 and Appendix A, respectively. One hundred percent survival occurred in the control exposure. Control fish had an average total length of 34 m,
and an average wet weigbt (blotteddry) of 0.23 g at the end of the
test.
Loading rate during the toxicity test was approximately
0.15 g/L.
The 24, 48, 72, and 96 hour LC50s for fish exposed to PC 206CY are presented in Table 3. The 96 hour LC50 value is greater than 1,000 mg/L PC 206CF. No subletbanl effects were observed in any test vessel during the 96 hour exposure.
RSOAM A-O^
Table 2. Survival data fro& taxicit- togt
nominal Concentration
(no/L)
rep.
Number Alive Obr 24br 48hr 72br 96hr
Number Affected Obr 24br 48hr 72hr 96hr
0 (con) 1 2
150
1
2
250
1
2
400
1
2
600
1
2
1,000
1
2
10 10 10 10 10 10 10 10 10 10
10 10 10 10 10 10 10 10 10 10
10 10 10 10 10 10 10 10 10 10
10 10 10 10 10 10 10 10 10 10
10 10 10 10 10 10 10 10 10 10
10 10 10 10 10 10 10 10 10 10
0 00 0 0 0 00 0 0
0 00 0 0 0 00 0 0
0 00 00 0 00 00
00 00 0 00 00 0
00 00 0 00 00 0
00 00 0 00 00 0
Note: (con) control.
AncV*dLkZ &6d@@ d Muupm ,
@-Table 3. median lethal concentrations (LC50m),*frotmoxicity test
Exposure
period
LC50
24 hours )1,000 mg/L
95 percent confidence limit
licso calculation method
48 hours )1,000 mg/L
72 hours )1,000 ag/L 96 hours )1,000 mg/L
Viz.izrnz*cz@s,.,
OECD. 1984. Guidelinesfor Testing of CbORiCals. Section 2: Effects on Biotic Systems. Method 203, rishAcuteToxicityTest. AdoptedApril 4, 1984.
oeAn*ft kv.;
Appendix A. WATN OUALM DATJK RROM TOXICITY TTST .]Page;iig@oz-.]Le
iR@4 I!&,
Table A.l. Conductivity,plf,temperature, and dissolved oxygen concentration measured during toxicity test
Nominal concentration
(so/L)
Rep.
Conductivity (unho/cm)
0 24 48 72 96hr hr hr hr hr
0 (con) 1 2
150
1
2
250
1
2
400
1
2
600
1
2
1,000
1
2
$30 $30 840 840 850 230 $40 840 850 850
840 840 840 $50 $60 $40 840 940 $50 850
830 840 $40 $50 960 840 840 840 $50 860
$40 830 $40 $50 $60 840 840 $50 $50 860
840 $40 850 $50 960 840 840 850 850 $60
$40 850 850 950 860 $40 $50 $50 $60 $70
Note: (con) control.-!-.
PH -
0 24 48 72 96 hr br hr hr hr
7.7 7.3 7.1 7.1 7.4 7.7 7.4 7.1 7.1 7.4
7.7 7.7 7.6 7.4 7.5 7.7 7.7 7.6 7.4 7.5
7.7 7.7 7.6 7.4 7.5 7.7 7.7 7.7 7.4 7.6
7.7 7.7 7.7 7.5 7.5 7.7 7.7 7.7 7.5 7.5
7.7 7.7 7.7 7.5 7.5 7.7 7.7 7.7 7.S 7.5
7.7 7.7 7.7 7.6 7.6 7.7 7.7 7.8 7.7 7.6
zzk
Table A.l. Continued.
Nominal
concentration
(mg/L)
Rep.
Temperature (OC)
0 24 48 72 96 br hr br hr br
0 (con) 1 2
150
1
2
250
1
2
400
1
2
600
1
2
1,000
1
-
2
22.2 22.2 22.1 22.2 22.4 21.8 22.0 22.0 22.1 22.4
22.0 22.2 22.3 22.0 22.7 22.0 22.0 22.0 22.1 22.4
22.0 22.0 21.9 22.0 22.3 21.9 22.0 21.9 22.0 22.3
21.9 21.8 21.8 22.0 22.3 21.7.21.7 21.8 22.0 22.3
21.9 21.9 21.8 22.0 22.3 21.8 21.8 21.8 22.0 22.3
22.0 21.8 21.9 22.0 22.3 22.0 22.0 21.8 22.1 22.4
Note: (con) control.
Dissolved Oxygen (mg/L)
0 24 48 72 96 hr hr hr hr hr
8.1 8.1 7.2 7.0 6.8 8.1 8.0 7.3 7.2 6.8
8.0 8.0 6.5 6.4 6.2 8.0 7.9 6.5 6.4 6.3
8.4 8.0 6.5 6.5 6.3 8.4 8.0 6.5 6.5 6.4
8.4 8.0 6.5 6.5 5.2 8.4 7.9 6.5 6.4 5.5
8.4 8.0 6.7 6.6 5.2 8.5 8.0 6.7 6.6 5.7
8.5 8.0 6.7 6.5 5.7 8.5 8.0 6.7 6.6 5.2
