Document R6DqQv6DMqGrv4KKZD855Lm7

HAZLETON l a b o r a t o m e s a m e r g a . rs c . 3301 KINSMAN BLVD., P.O. BOX 7545 MADISON. Wl 53707 USA FINAL REPORT Karen Armstrong 3M _ Toxicology Services Building 220-2E-02 St. Paul, MN 55144-1000 Sample: T-4416 HLA Number: 91201739 Enclosed: Acute Oral Toxicity Study in Rats (OECD Guidelines) o Key Personnel o Methodology o Summary of Results o Individual Pathology Comments o References o Pathology Report o Raw Data/Appendix Signed: Steven M. Glaza Study Director Acute Toxicology PHONE (608) 241-4471 FACSIMILE (608) 241-7227 TELEX TLX 703956 HAZRAL MDS UD Date a OOffNHM O Laboratory Sanncaa Company HAZLETON LABO R ATOPeS AMEPGA.*''JC 3301 KINSMAN BLVD.. P.O. BOX 7545 MADISON. W l 53707 USA HLA Number: 91201739 Sample: T-4416 Acute Toxicology Steven M. Glaza Study Director Section Supervisor Steven R. Sorenson Study Coordinator Patricia Padgham Group Leader Sharen L. Howery Report Coordinator Oualitv Assurance Deborah L. Keller Manager Page 2 KEY PERSONNEL Laboratory Animal Veteri Cindy J. Cary, DVM Diplomate, ACLAM ian Anatomical Pathology Thomas E. Palmer, PhD Anatomical Pathologist Dennis Hoffman Group Leader Necropsy Anne Mosher Group Leader Pathology Data PHONE (608)241-4471 FACSIMILE (608) 241-7227 TELEX TLX 703956 HAZRAL MDS UD C O R N IN O LAborwory Smr*cm Company HAZLETON LABORATCW ES A M EH CA.rse. 3301 KINSMAN BlVD., P.O. BOX 7545 MADISON, W l 53707 USA HLA Number: 91201739 Sample: T-4416 Page 3 METHODOLOGY Objective: To assess the acute oral toxicity produced when a test material is administered by oral gavage to rats.1 Test Material: T-4416 Physical Description: Purity and Stability: Storage and Retention: Safety Precautions: Clear colorless liquid Sponsor assumes responsibility for purity and stability determinations (including under test conditions). The test material was stored at room temperature. Any unused material will be returned to the Sponsor according to HLA Standard Operating Procedure. Normal handling procedures were used according to HLA Standard Operating Procedure. Test Animal: Adult albino rats, Crl:CDBR, were procured, separated by sex, maintained in group cages in temperature- and humidity-controlled quarters, provided continuous access to Rodent Chow 5001, Purina Mills, Inc., and water, and held for an acclimation period of at least 7 days. Animal husbandry and housing at HLA comply with standards outlined in the "Guide for the Care and Use of Laboratory Animals".2 If variations from the prescribed environmental conditions existed, they were documented and considered to have no effect on the study outcome. No contaminants were expected to have been present in the feed or water which would have interfered with or affected the results of the study. Acclimated animals were chosen at random for the study. Five male and five female rats, weighing from 204 to 299 g, were used for each dose level. The study consisted of three dose levels (0.2, 1.0 and 5.0 g/kg of body weight). Test animals were housed by sex in groups of five and identified by animal number and corresponding ear tag. Food and water were available ad libitum throughout the study, except for an overnight period just before test material administration when food, but not water, was withheld. Reason for Species Selection: Historically, rats have been used as a representative of a rodent species and are preferred by various regulatory agencies. Preparation and Administration of Test Material: The test material was administered as received. An individual dose of the undiluted material was calculated for each animal based upon its fasted body weight and administered by gavage. The dose volume of the test material varied for each dose level based upon the bulk density of the material of 1.28 g/mL. PHONE (608) 241-4471 FACSIMILE (608) 241-7227 TELEX TLX 703956 HA2RAL MDS UD a coratvitryo Laboratory Sarvcaa Company HAZLETON LABORATORIES AM ERCA. <NC 3301 KINSMAN BLVD., P.0. BOX 7545 MADISON. W l S3707 USA HLA Number: 91201739 Sample: T-4416 Page 4 Reason for Route of Administration: Historically, the oral route has been the route of choice for administering a known amount of test material. Observations: The animals were observed for clinical signs and mortality at 1, 2.5 and 4 hours after test material administration. The animals were observed daily thereafter for 14 days for clinical signs and twice daily for mortality. Animals were weighed just before test material administration. Surviving animals were weighed again at Day 7 and at termination of the experimental phase or at death (when survival exceeded 1 day). Pathology: All animals whether dying on test or euthanatized at termination of the experimental phase were subjected to a gross necropsy examination and all abnormalities were recorded. After necropsy, the animals were discarded and no tissues were saved. Statistical Methods: The LD5Q value for males, females and the combined sexes was calculated based upon the observed mortality using a computer program utilizing a modified Behrens-Reed-Muench Cumulant Method. No other statistical analysis was performed. Location of Raw Data and Final Report: The raw data and a copy of the final report will be retained in the archives of HLA. PHONE (608) 241-4471 FACSIMILE (608) 241-7227 TELEX TLX 703956 HAZRAL MDS UD C C W W mO Laboratory Sorvicaa Company HAZLETOIM LABORATORIES a m e h c a . MC 3301 KINSMAN BLVD., P.0. BOX 7545 MADISON, W l 53707 USA HLA Number: 91201739 Sample: T-4416 Page 5 SUMMARY OF RESULTS Test Animal: Albino Rats - Crl:CDBR Source: Charles River Laboratories, Inc., Portage MI Date Animals Received: 12/04/89, 12/18/89 and 01/08/90 Method of Administration: Oral Gavage Start Date (In-life): 12/28/89 End Date (In-life): 02/16/90 Estimated Oral LD50 : Male - 1.8 g/kg of Body Weight 95% Confidence Limits - 0.7 to 4.9 g/kg Female - 0.6 g/kg of Body Weight 95% Confidence Limits - 0.2 to 1.5 g/kg Sexes Combined - 1.0 g/kg of Body Weight 95% Confidence Limits - 0.4 to 2.4 g/kg Sex Male Female Dose Level (a/ko) 0.2 1.0 5.0 0.2 1.0 5.0 Averaae Bodv Weiahts (a) Initial Dav 7 Terminal 290 332 272 264 274 -- 362 315 -- 211 247 233 257 230 -- 254 268 -- Mortality (Number Dead/Number Dosed) 0/5 1/5 5/5 0/5 4/5 5/5 PHONE (608) 241-4471 FACSIMILE (608) 241-7227 TELEX TLX 703956 HAZRAL MDS UD . O CW n im O U bow ay S m i Company  HAZLETON L A B O R A T O H E S A M E O C A , IMC. 3301 KINSMAN BLVD., P.O. BOX 7545 MADISON, W l 53707 USA HLA Number: 91201739 Sample: T-4416 Observation Appeared normal Clinical Signs (Number of Animals Affected) Hour __ _________________ Day. 1.0 2.5 4j.Q I 2 3 4 5 6 7 8 9 10 11 12 11 14 O ro Q O Males 5 5 5 555555555 5 5 5 5 5 Appeared normal Females (0-2 q/kq) 5 5 5 555555555 5 5 5 5 5 O o Males Appeared normal 5 5 5 521112333 3 3 3 4 4 Hypoactivity 0 0 0 032222111 0 0 0 0 0 Red-stained face 0 0 0 0 1 2 2 2 1 1 1 1 1 1 0 0 0 Yellow-stained urogenital area 0 0 0 0 1 3 3 3 1 1 1 1 1 1 1 0 0 Ataxia 0 0 0 000001111 0 0 0 0 0 Thin appearance 0 0 0 000001111 1 0 0 0 0 Di arrhea 0 0 0 0000000 11 0 0 0 0 0 Death 0 0 0 0 10000000 0 0 0 0 0 Females M . O q/kg) Appeared normal 5 5 5 511111111 1 1 1 1 1 Hypoactivity 0 0 0 022 100000 0 0 0 0 0 Red-stained face 0 0 0 0 2 2 1 0 0 0 0 0 0 0 0 0 0 Ataxia 0 0 0 00 1000000 0 0 0 0 0 Prostration 0 0 0 000 100000 0 0 0 0 0 Death 0 0 0 0 12 100000 0 0 0 0 0 PHONE (608) 241-4471 FACSIMILE (608) 241-7227 TELEX TLX 703956 HAZRAL MDS UD O O b n w o L u x x a m y S Comoan, HAZLETON LABORATORIES A M E R C A . rsC3301 KINSMAN BLVD., P.O. BOX 7545 MADISON. W l 53707 USA HLA Number: 91201739 Sample: T-4416 Clinical Signs (Continued) (Number of Animals Affected) Observation Hour 1.0 2.5 4.0 Mali Dav Appeared normal Diarrhea Hypoactivity Red-stained face Lacrimation Ataxia Death 10 44 04 02 00 00 00 00 41 41 41 10 01 14 * - One animal found dead after the morning observation. Females (5.0 q/kq) Appeared normal Diarrhea Hypoactivity Lacrimation Ataxia Red-stained face Death 20 33 13 02 01 01 02 0 02 02 02 01 01 01 2- Page 7 PHONE (608) 241-4471 FACSIMILE (608) 241-7227 TELEX TLX 703956 HAZRAL MDS UD OOANtfM O Laboratory Sanwcao Company HAZLETON LABORATCWES AMERCA-fsC3301 KINSMAN BLVD., P.O. BOX 7545 MADISON, W l 53707 USA HLA Number: 91201739 Sample: T-4416 INDIVIDUAL PATHOLOGY COMMENTS Dose Level: 0.2 g/kg of Body Weight Animal Test Dav Number Sex Died Sacrificed NecroDSV Observation C50366 M - 14 No visible lesions C50367 M - 14 No visible lesions C50363 M - 14 No visible lesions C50369 M - 14 No visible lesions C50370 M - 14 No visible lesions C50501 F - 14 No visible lesions C50502 F - 14 No visible lesions C50503 F - 14 No visible lesions C50504 F - 14 No visible lesions C50500 F 14 No visible lesions Page 8 PHONE (608) 241-4471 FACSIMILE (608) 241-7227 TELEX TLX 703956 HAZRAL MDS UD a OOUMKMO Laboratory San o cai Company HAZLETON l a b o r a t o r ie s A M E P C A , MC 3301 KINSMAN BLVD., P.0. BOX 7545 MADISON. W l 53707 USA HLA Number: 91201739 Sample: T-4416 Page 9 INDIVIDUAL PATHOLOGY COMMENTS (continued) Dose Level: 1.0 g/kg of Body Weight Animal Test Dav Number Sex Died Sacri ficed NecroDsv Observation C49861 M - 14 No visible lesions C49862 M - 14 No visible lesions C49863 M - 14 No visible lesions C49864 M 2 - Stomach, Glandular Mucosa: diffusely red C49860 M - 14 No visible lesions C49921 F 3 - Stomach, Glandular Mucosa: diffusely red C49917 F 2 - No visible lesions C49918 F 4 Perinasal Discharge: red Stomach, Glandular Mucosa: multiple dark areas, up to 2 x 4 mm C49919 F 3 - Stomach, Glandular Mucosa: diffusely red C49920 F - 14 No visible lesions PHONE (608) 241-4471 FACSIMILE (608) 241-7227 TELEX TLX 703956 HAZRAL MDS UD C O R N IN O Laboratory Sorvic-- Company 0 's j? ' HAZLETON LABO RATCW ES AM ERCA.IM C. 3301 KINSMAN BlVD., P.O. BOX 7545 MADISON. W l 53707 USA HLA Number: 91201739 Sample: T-4416 Page 10 INDIVIDUAL PATHOLOGY COMMENTS (continued) Dose Level: 5.0 g/kg of Body Weight Animal Number Sex Test Dav Died Sacrificed NecroDSV Observation C49191 M 1 - No visible lesions C49192 M 1 - No visible lesions C49193 M 1 - Stomach, Glandular Mucosa; multiple brown areas, up to 3 x 1 mm C49194 M 0 - Stomach, Glandular Mucosa: diffusely dark red C49490 M 1 - No visible lesions C49131 F 0 - Stomach contents: clear white fluid C49132 F 1 - Stomach, Glandular Mucosa: multiple brown areas, up to 4 x 2 mm C49128 F 0 -, Stomach Contents: clear white fluid C49129 F 0 - Stomach Contents: clear white fluid C49130 F 1 - No visible lesions PHONE (608) 241-*471 FACSIMILE (608) 241-7227 TELEX TLX 703956 HAZRAL MDS UD O O A n in o Laboratory SanncM Company HAZLETON LABO R ATOneS AM EBCA. IVIC. 3301 KINSMAN BLVD.. P.0. BOX 7545 MADISON. W l 53707 USA HLA Number: 91201739 Sample: T-4416 Page 11 REFERENCES 1. "Acute Oral Toxicity," Organisation for Economic Cooperation and Development's Guidelines for Testing of Chemicals, Section 401 (adopted May 12, 1981). 2. NIH Publication No. 86-23 (revised 1985). 3. Thakur, A. K. and Fezio, W. L., "A Computer Program for Estimating LD50 and its Confidence Limits Using a Modified Behrens-Reed-Muench Cumulant Method," Drug and Chemical Toxicology, 4(3):297-305 (1981). PHONE (608) 241-4471 FACSIMILE (608) 241-7227 TELEX TLX 703956 HAZRAL MDS UD a OOnrsMMQ laboratory Sarvtc-- Company HAZLETON LABC3R A T O W E S A M E f C A . rjC . 3301 KINSMAN BLVD., P.0. BOX 7545 MADISON. Wl 53707 USA HLA Number: 91201739 Sample: T-4416 Page 12 PATHOLOGY REPORT There were 10 rats (5 males, 5 females) from each of 3 dose levels (0.2, 1.0, and 5.0 g/kg) necropsied. The animals that died on test (DOT) were refrigerated and necropsied within 24 hours, usually the same day. All surviving animals were euthanatized and necropsied at the termination of the study. The dose level, day of death, and gross observations recorded for each animal are on page 8 through 10 of this report. All animals in the 5.0 g/kg dose group died on test. At necropsy, the only changes observed were in the animals that died on test. The most frequently recorded observations pertained to the content and coloration changes in the mucosa of the stomach. These findings possibly represent test material mixed with ingesta, postmortem change, or both. All remaining observations in the DOTs were considered postmortem change, incidental, and unrelated to the test material. There were no visible lesions in the sacrificed animals. Pathologist (91201739.cjr) Date PHONE (606) 241-4471 FACSIMILE (608) 241-7227 TELEX TLX 703956 HAZRAL MDS UD C O R N IN O Lab o rato ry S arw eai Com pany HLA: PERSONNEL SIGNATURE SHEET ACUTE TOXICOLOGY Name Job Title Signature Initials Becky Beckwith Lab Animal Technician 6ft- Ann Bursaw Anthony Cass Cindy J. Cary, DVM Kari Gassen Sr. Clerk phodBeA&zss r Lab Animal Assistant Lab Animal Veterinarian tOr^ta/Tyuu C cul>l Cwuta Sr. Lab Animal Assistant I4y\_ prnj&- c\c Steven M. Glaza Ben Haley ' Section Supervisor Sr. Lab Animal Assistant SML Jeff Hicks Sharen L. Howery Lab Animal Technician Research Assistant sP,L Jim Jirschele Wayne Madison Lab Animal Assistant Senior Section Supervisor { O- H J riJ L i (j Bud McDonald Eileen McConnell Henry Mikula Albert Oleson Patricia Padgham Sr. Lab Animal Assistant A u A /V d )& \c & L Administrative Clerk Lab Animal Assistant Sr. Lab Animal Assistant C )$U+r\ Group Leader 2lZ<-* .-rm-r-v .? Z7 * 1 Jk2_ Jane Pol now Lab Animal Technician >41 f <^nii'Y)L Steven R. Sorenson Study Coordinator X'a.Tot*-- -- J Dawn Thieding t Annette R. Turner Clerk Sr. Clerk 4^ PtR /L,^Ui} > 7-- - i2a - (09/11/89) DOSE ADII IHISIRATI Otl/OOl)Y HElCIU/MOni. ECOOD HLA: <4; Zeo i l l ? Study T lt le i Acuta Oral lo x lc lty Test H a le rla ll___f_ >4 ^ I p Vch le lo ! A J A Bulk D ensity: ) . 2 ? lg /a l) S p e cle j/S tra ln : A lbino Rat/Snraouc-Dawlcy Sunrco; Cliarlos H lvor Dale Received: / ~ g ' 2 P .2-Doit Laval! O Ig/kn) Fasted Dita/T Ino/Iaclinlc Ian lIfo/ 3:o5~!/-- SjM Ko"llo-_Z^-2r Kouta of A d ia ln lttn it Ion! Oral Givano Dose Volume O, 1Cl> (ail/kq) Sex: fila le)Fcioala O G 0 oV Ji Prcfasted Body Welqnt |q) NA rasted Uody Uelqlit (q) Actual Dose ( a il Day 7 Uody Uelqlit (q) iH o.o S M Sj VZ^ 0-0<> V<-4 iV ? .0 ^ 3yi in o aS 32? Day 14 Body Ualnlit (q) 3S<- 32^ Dead Uody Uelobi (o) Sexl Ila lo/FenatiD c S c I O fo Z zfZ oZ cS c4 Prcfastcd Uody Uoli|lit In) lasted Uody Ueloht (q) Actual Duse (ml) Day 1 Uody Uelqlit (q) lljy 14 Uudy Ualqht (q) NA l - \ ? i a >4 (7 .O ' 1) 7-0 3 IT ? v O z i o 14 L> 10*4 o .o 3 ?dl 24 ( 111 c .o 3 ?5C* IC (~ \Uose T1u: ^ 3 % le d ili le Ian nu -X 2<?V o . o "S \= \ \ ________ (V G IJ J lT > 2^ \ ________ Q t l _V m \ Dnsa TIaioi 9o S 'o o Tedili le Ian m -A -- 2oG> v. c-3 ZH l \ \ \ ________ \ m (B /-J W m 1990 Date Scala Used KTrOPI V- HA -------------------------- > XA A V ie S I-!-? Ver l i led by? - -9 0 Z Z T -X 5 Z Z .F il yoyoun Data Scale UsedKTron V t- HA --------------------------- > V t. z/z . A V /C . S 'z t- S ,0 'T S ^ 9 O 32JS- Dead Undy UeIqht (q) \ HOATALTY (MO. DIED/HO. DOSE) Hose un1evel ! 0 /t9 L Sex Hours i n - \ -- I-- 1 AM---- PH AM PII m -- ------------------------ 5ISH7 lily -- -- ~T -V ni /nr"PH r n i AH hi ah-- m AH PII All IT T2 13 ---FT" lotal fu AM PII AH-- Fl I AH-- ffl Ail Fh w T Fh "cT a a isO . 2 c T 7 f I 5A k % Is /s I s A% %g L ft ha k EtPA #C .'Z ? m i k A 7s It k 7 A % L A i c7a 2 t iji HA is- Cilstl'VJkyj CHlech. c d 3e 10= a 1 W li 3ti (d %i ~2L ni OL SV ,sv -7/ HA & / Tim e oat. 1990 NA ft , ._laM W W W h< y-)ffCD )lo i n Ja % % Yi T, T L ft fj^yo f jt V ^ ` r.i* U " m 2//// % 2 V Ib i|Vl L//<-- JoO fi]) 1 r> > l lo Uj /II 7ft % yJ HA HA Vk. HA - Hot Applicable Reviewed Dy , Z W Data Oose level: .'Z i' OBSERVATIONS (Individual) Test material: ~7~'- IC, r( HLA: 3 / 7 ^ 0 / l 3 f Surviving animals submitted for terminal necropsy. Technician i W * Date ___ 1 Surviving animals designated for sacrifice and discard. Technician HA__________ Date NA_______________ Indicates condition exists SI Slight. - Condition not evident. * Found dead, P.H. check. NA Not applicable Reviewed by: 7 7 / _____ Date: ________ Dose level : O ,'~L OBSERVATIONS (Individual) Test material: ~ 1"- Lfty / C- HLA: 4 ILO i l 3 7 Surviving anima 1s submitted for terminal M *necropsy. Technician Date 1 - / ^ 9 o Surviving animals designated for sacrifice and discard. Technician NA__________ Date NA_______________ Indicates condition exists SI Slight. - Condition not evident. * Found dead, P.M. check. NA Not applicable Reviewed by: .7?/_____ Date: _______ S - J J fo DOSE AUII1HISIRAI lOtl/DOUY ME ICIII/HOfllA. i RECORD HLA: 7 / Z o / ~>3 7 StuJy l i t i c i Acuta Or! o u lc lty l a t i Hater la i! ~7~ - 4 *4 I Veli te la : n A Bulk D cnllty.' I 2 . & I g/icA-) Speci es/S tra In : Albino Rt/S[iroue-Uawley Suiirco; Charles Ulvor Date Rccalveili l~L 'l$ Uosa Leve! : / Q lo/ka) F a sle J Dste/T Ino/Taclmlc lan I ~ / *3 ' ii.a i./ Roow llo._Jj_^ _Z, Dose level : OBSERVATIONS (Individual) /. O ^ / Aey.________________ Tesi material: / '- I Ce> HLA: I Z O I I Z ? Surviving animals submitted for terminal a inecropsy. Technician Date / -* Surviving animals designated for sacrifice and discard. Technician NA__________ Date NA __________ Indicates condition exists SI Slight. Condition not evident. * Found dead, P.H. check. NA Not applicable Reviewed by: J 7 / _____ Date: ________ j -JJ - ? 0 Dose level: / . O - / A I OBSERVATIONS (Individual) Test material: ~ / - *-} *-! I C>________ HLA: ^ / l o H 3 ^ Surviving animals submitted for terminal necropsy. n , Technician W ______ Date j -t3 Surviving animals designated for sacrifice and discard. Technician M*__________ Date NA ___________ Indicates condition exists SI Slight. - Condition not evident. * Found dead, P.M. check. NA Not applicable Reviewed by: J ? / _____ Date: ______ ____________ f DOSE AUIIIHIS1RATIOII/DOOY WEIGHT/HOniAL. . i RECORD HLA: O l Z O i n l ^ Study I l t l a l Acuta Orel lo x lc lty Ale st H a te r t* I! ~ T ~ * 4 * 4 / 6 ? _________________ Veli Ic To:______A_/_______ Bulk D ensity: /1 % Ig/aaL) S p e d e i/ S tra ln : Albino Ral/Snra.iue-Hawley Sunrco: Charles II Ivor Date Received: )2 pose Le vel: S ~ . & lo/ka) Fasted D ate/Ilno/Taclinlclan 1 2 - 1 1 / 2 `IS'v-*-/ Z2/~(____ **00" Ho Houta of A Jd ln ls tn it Ion: Oral Cavano Dose Volume^ ^ / (ad-Ag) Prcfasted Body Welqht (q) l asted Uody Welqlit (q) Actual Dose (niL) Uav 7 llody Uclqlit lq) Day M Body Welqlit lq) Dead Body Welqlit In) mi NA i.O OxJ 11 IW Sex: fila leTfciaa la / |4 Z j l ^ wo VFi / .1 hO OtrJ O r o J ii-vfiy il i n n V\$ Vi'l' / . ) 1. 1 od Q&J} I2-V-& " W Ahi ii c Hose Tliiu: \a tori-- \ \ \ \ K \ \ Dm* Volume 1> 1 (r i.A o ) 1lM Sex: Malo/FcmaliD ty V L 4\18 Dnse 1 Uio: 0-1 VAf' \o \ P rcfe stcu Uody Wolilit |o ) NA -5 = , l asted Uody UeIqlit (q) Actual Duse (ml) Way 7 llody Welqlit (q) Way H Uudy Walqht (q) Dead Uody WeIqht (ql u\o VIM 13^ V\2 * o f ) 1] o.9>% o . ^ o o fiZ t w ' ThddA&xJ d)<?a.g 1VWll-frtf n - n . 0 H-li-tf it-isrt m Z\ ff Ot'i 0 1 4 V o f \ _________ \ \ le d in lc Ian w i7)1 198^ o Date Scale Used KTrO fl (PCv^i-v e r r o r ' fl/4 U l S ' i ' i "hz HA ---------------------- > >% 'V i s~iz& Ver If led by: SIS' U.st sl A^cJ #4- P M. itici 1 H IS S I f~~ ' -- -- -- +A~ i o a -- . fyd n lectin Ic Ian w i onQ/oa Date Scale UscdKTron 'V U HA ----------------------- > ad Q , li % x%/ u s 5 'ZC8Ver 11lot! l>yi - J * - f U.JS-S3____ Hose 1evel iadtaL 6-0 Sex cT Wi s~.o ? _____ lech. Tine Date 1989& nrWiH __ 1% IIA - Not Applicable I TH HORTALITT (HO. P IEP /IIO. DOSE! I t i ii l y bay 9 U T 7JI-- PM wi ni AH-- I'll Reviewed Dy J ? / Pate j>-J& f<2 -----F3 14 lota 1 All I'M T m HA * / s HA HA l i HA Nfl HA " h i (D yS~,Dose leve 1: a, OBSERVATIONS (Individual) Test material: ~7~~- *-/*-// HLA: ch t o r i ' l C} () t-'f' to As- /x z*?- 2r9 - Surviving animals submitted for terminal necropsy. . Technician N Date AJft______ - Surviving animals designated for sacrifice and discard. Technician NA__________ Oate NA_______________ Indicates condition exists SI Slight. - Condition not evident. * Found dead, P.H. check. NA Not applicable Reviewed by: _____ Date: ___ HLA: ^17,01 I l > 9 Dose level S ' . a OBSERVATIONS (Individual) Test material T~ - S W / C Ha 1g/Fema 1 Anima 1 Pre- Hours Study Day Number Observations Appeared Normal dose 1 12.5 4 y --- 1 l\2 3 1 4 5 6 7 1 8 9 M o l i l i 12 1--13 1i--14 >1- (/ifrW ____________ Jy \ Al ____ ____________ y\ ,/ \ T VY v \V Appeared Norma 1 ('jlAVr li-._____________ Hyfy.-rnc ____________ /Hr-XI4 _______________ I W ----------- *-- -- i/ y -- i/ y - </ y -- 1/ -- J <W- Appeared Norma 1 O i n p n r l ' f ____________ K y -hi ------Appeared Normal `l l Z ? t/ _ -- y1 , ,/ Y T\ \ y-- J \ r r T T i Appeared Normal s / _ --- oj- (9jd inrks& .______________ fAyfan >.hvf_____________ ~T y J y- yy K < J s - b , n <rd ______ i/ y1 -- O leS -C _iZ Deaths Collected by Date 1989 /AO 4L wN A , (?> 2. J__ T. ry-1 ^Vz3 w IM Y\ s T V's sV YA\ \YY \Y Y VA AYY V-- ~Y --N Surviving animals submitted for terminal necropsy. . Technician f ^ n _____ Oate Aj/\__________ Surviving animals designated for sacrifice and discard. Technician NA__________ Date NA_______________ Indicates condition exists SI Slight. - Condition not evident. * Found dead, P.H. check. NA Not applicable Reviewed by: J?/ Date: ________ -yj?-9/)