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FINAL REPORT
A R 'XSr-0363
Roger G. Perkins, PhD
3M Toxicology Services Building 220-2E-02 St. Paul, MN 55144-1000
HWI Number: 40200470
Study Title:
Primary Eye Irritation/Corrosion Study of T-5898 in Rabbits (OECD Guidelines)
\
A e p a -o t s
000811806O OOGllOGO i
Signed:
Steven' M. Glaza Study Director Acute Toxicology
Date
COJ> XCCj-j t ro
Phon-
600-241-4471
F O R E >: P E i; $ j- f-/ A ! L P E L. J V E a V: 3 3 0 7 I! I N S M A I'J
F r* > H 1L E V A R D, W A P
005766
HWI Number: 40200470 Sample: T-5898
Acute Toxicology Steven M. Glaza Study Director Manager Steven R. Sorenson Study Coordinator Patricia Padgham In-life Supervisor Rose M. Bridge Report Supervisor
Page 2
KEY PERSONNEL
Laboratory Animal Medicine Cindy J. Cary, DVM Dipl ornate, ACLAM Supervisor
CONTENTS
Key Personnel Objective Test Material Test System Procedures Summary of Results Discussion References Appendix - Raw Data
Page
2 3 3 3 4 6 10 10 11
005767
HWI Number: 40200470 Sample: T-5898
Page 3
OBJECTIVE
The objectiv of this study was to assess the relative level of irritation/ corrosion produced following a single exposure of a test material to one eye of albino rabbits.1
All procedures used in this study are in compliance with the Animal Welfare Act Regulations. In the opinion of the Sponsor and study director, the study did not unnecessarily duplicate any previous work.
TEST MATERIAL
Identification
The test material was identified as T-5898 and described as a clear, lightyellow liquid.
Purity and Stability
The Sponsor assumes responsibility for purity and stability determinations (including under test conditions).
Storage and Retention
The test material was stored at room temperature. Any unused test material will be returned to the Sponsor after issuance of the final report according to Hazleton Wisconsin (HWI) Standard Operating Procedure (SOP).
Safety Precautions
The test material handling procedures were according to HWI SOPs and policies.
TEST SYSTEM
Test Animal
Adult albino rabbits of the Hra:(NZW)SPF strain were procured from HRP, Inc. and maintained at the Hazleton Wisconsin facility at 3802 Packers Avenue, Madison, Wisconsin. Animal husbandry and housing at HWI comply with standards outlined in the "Guide for the Care and Use of Laboratory Animals".2 The animals were individually housed in screen-bottom cages in temperature- and humidity-controlled quarters, provided access to water ad libitum and a measured amount of Laboratory Rabbit Diet HF #5326, PMI Feeds, Inc., and held for an acclimation period of at least 7 days. The feed is routinely analyzed by the manufacturer for nutritional components and environmental contaminants.
005768
HWI Number: 40200470 Sample: T-5898
Page 4
Samples of the water are periodically analyzed by HWI. There were no known contaminants in the feed or water that would have interfered with or affected the results of the study.
Three female acclimated animals, weighing from 2,628 to 2,722 g, were selected and maintained during the study in the same manner as for the acclimation period. If variations from the required temperature and humidity conditions existed, they were documented and considered to have had no adverse effect on the study outcome. The animals' eyes were examined on the day before test material administration using sodium fluorescein dye procedures. Only those animals with no sign of ocular injury or irritation were used. Animals were identified by animal number and corresponding ear tag.
Justification for Soecies Selection
Historically, the New Zealand White albino rabbit has been the animal of choice based on its large orbit and nonpigmented iris.
PROCEDURES.
Preparation of Test Material
The test material was administered as received. The pH of the test material was determined to be 7.6.
Treatment
Each rabbit received 0.1 mL of the undiluted test material placed into the everted lower lid of the right eye, with the left eye serving as the untreated control. The upper and lower lids were gently held together for 1 second to prevent loss of material and then released. The eyes of the rabbits remained unflushed.
Reason for Route of Administration
Historically, the ocular route has been the route of choice based on the method of Draize.3
Observations
The treated eyes were observed for ocular irritation at 1, 24, 48, 72, and 96 hours and Days 7 and 14 after treatment. Irritation was graded and scored according to the Draize technique. Sodium fluorescein examinations were used to aid in revealing possible corneal injury at 72 hours and Days 7 and 14.
005769
HWI Number: 40200470 Sample: T-5898
Page 5
Animals were weighed just before test material administration and at weekly intervals throughout the study.
Termination At termination of the experimental phase, all animals were designated to be euthanized and discarded.
Statistical Analyses No statistical analyses were required by the protocol.
Location of Raw Data. Records, and Final Report The raw data, records, and a copy of the final report will be retained in the archives of HWI in accordance with HWI SOP.
00S770
HWI Number: 40200470 Sample: T-5898
Page 6
SUMMARY OF RESULTS
Test Animal: Albino Rabbits - Hra:(NZW)SPF Source: HRP, Inc., Kalamazoo, MI Date Animals Received: 02/17/94
Experimental Start Date: 02/24/94 Experimental Termination Date: 03/10/94
Average Primary Eye Irritation Scores*
Observation Period
Average Score
1 Hour 24 Hour 48 Hour 72 Hour 96 Hour
Day 7 Day 14
24.3
26.7 15.0 10.3
7.3 4.3 0.0
* The average primary eye irritation score is the total eye irritation score for all the animals divided by the number of animals (3) at each
observation period.
005771
HWI Number: 40200470 Sample: T-5898
Page 7
Animal Number
F49828u F49829t F49795u
Table 1
Individual Eye Irritation Scores
Cornea AB
Iris C
Con.iunctivae DEF
1 Hour
1 1 l1 2 2 2C 1 2 l1 2b 3 2C 1 1 l1 2b 2 2C
Mean
Total Score*
22.0 29.0 22.0
24.3
24 Hours
F49828
1
1
l1
2b 2
Ie 20.0
F49829
1
3
l1
2b 3
t 34.0
F49795
1
1
l1
2b 3
3C 26.0
Mean
26.7
F49828
0
0
F49829 1J 2
F49795 lj 1
48 Hours
01 l1 2b l1 2
0 2 1
Mean
0 2C 0
2.0 27.0 16.0
15.0
Cornea
Iris
Coniunctivae
A - Degree of opacity B - Area of involvement
C - Degree of iridai irritation
D - Redness E - Chemosis F - Discharge
* Total score = (A x B x 5) + (C x 5) + [(D + E + F) x 2],
b Blanching, c Clear discharge, d Purulent discharge, i Injected.
j Corneal epithelial peeling. t No pain response after test material instillation, u Excessive pawing at the treated eye after test material instillation.
005772
HWI Number: 40200470 Sample: T-5898
Page 8
Animal Number
F49828 F49829 F49795
Table 1 (Continued)
Individual Eye Irritation Scores
Cornea AB
Iris C
D
72 Hours
Con.iunctivae EF
000
10 0
1 1 l1 2b 1 Ie
1J 1
0
210
Mean
Total Score*
2.0 18.0 11.0
10.3
96 Hours
F49828
0
0
0
F49829
1
1
0
F49795
1
1
0
0 0 0 0.0 2 1 0 11.0
.2 1 0 11.0
Mean
7.3
F49828
0
0
F49829
0
0
F49795 l3 1
Cornea
A - Degree of opacity B - Area of involvement
Dav 7
0 00 0 10 0 21
Mean
Iris
C - Degree of iridai irritation
* Total score = (A x B x 5) + (C x 5) + [(D + E + F) x 2]. b Blanching, c Clear discharge, i Injected. j Corneal epithelial peeling.
0 0.0 0 2.0 0 11.0
4.3
Conjunctivae
D - Redness E - Chemosis F - Discharge
005773
HWI Number: 40200470 Sample: T-5898
Page 9
Animal Number
F49828 F49829 F49795
Table 1 (Continued)
Individual Eye Irritation Scores
Cornea AB
Iris C
D
Day 14
Con.iunctivae EF
000 000 000
000 000 000
Mean
Total Score*
0.0 0.0 0.0
0.0
Cornea
A - Degree of opacity B - Area of involvement
Iris
C - Degree of iridai irritation
* Total score = (A x B x 5) + (C x 5) + [(D + E + F) x 2].
Con.iunctivae
D - Redness E - Chemosis F - Discharge
Table 2 Sodium Fluorescein Examination
Animal
Observation Period
Number Sex Preinitiation 72 Hour
Dav 7
Dav 14
F49828 F49829 F49795
F F F
NEG NEG NEG NEG
NEG NEG NEG NEG
NEG
POS (5%)
POS (5%)
NEG
NEG Negative stain retention. POS Positive stain retention (area of cornea involved).
005774
HWI Number: 40200470 Sample: T-5898
Page 10
DISCUSSION
The test material, T-5898, when evaluated for its primary eye irritation potential in rabbits, produced corneal and iridal involvement and moderate to severe conjunctival irritation. All treated eyes had returned to a normal appearance by Day 14 after treatment.
REFERENCES
1. "Acute Eye Irritation/Corrosion," Organisation for Economic Cooperation and Development's Guidelines for Testing of Chemicals, Section 405 (adopted May 12, 1981).
2. NIH Publication No. 86-23 (revised 1985).
3. Draize, J. H., "Eye Mucosa," In: Appraisal of the Safety of Chemicals in Foods, Drugs and Cosmetics - Dermal Toxicity, Association of Food and Drug Officials of the U.S., pp. 49-50 (1959).
00S77S
HWI Number: 40200470 Sample: T-5898
APPENDIX Raw Data
Page 11
005776
PERSONNEL SIGNATURE SHEET ACUTE TOXICOLOGY
hwi: ^ O Z O O h t T a
Name
Job Title
Signature
Rose M. Bridge
Report Supervisor
Anthony Cass
Lab Animal Technician
Cindy J. Cary, DVM
Lab Animal Veterinarian
Donna J. Clemons, DVM, MS Lab Animal Veterinarian
John A. Disch
Lab Animal Caretaker
Charles W. Fritz
Lab Animal Technician
K a n Garfoot
Lab Animal Technician
Steven M. Glaza
Manager
Sts,
Kevin Grossman
Lab Animal Caretaker
Jeff Hicks
Lab Animal Technician
Sharen L. Howery
Research Assistant
Wayne A. Madison
Supervisor
O0ly*L //? .
Doug McConnell
Lab Animal Technician
Eileen McConnell Bud McDonald
Staff Assistant Study Coordinator
& < d PV\o?7at
Albert Oleson
Lab Animal Caretaker
AQ
Patricia Padgham
In-life Supervisor
-
Steven R. Sorenson
Study Coordinator
Annette R. Turner
Staff Assistant
JtL.
Tamra L. Walker
Staff Assistant
Lana M. Weeden dtefAcr M. Itither
Staff Assistant
^LiaSH.
U
UnrGrtalP^M hr
/J. D ^lL - k L
VrAo \ W \ m A Gxv^Aate r ~ ~ ^XJl 1.
(G2/10-06-93)
005777
HWI Mo.: A 4 2 Q C y - j n n
TEST MATERIAL ADMINISTRATION RECORD
Test Material: -r- -Sgff_____________________________________________
Physical Description:
L J G J - l r ' \j^JLL.CM j l j G>LU A ___________
pH Result: 7-k>_____ with Corning pH Meter No. 05510
pH was determined on:
undiluted material / .A dilution of v % v a /m a
U - Unable to determine pH due to m o __________________________ _______
Storage conditions of test material:
______
Approximately 5 mTnDtw-banftJ:est material instillation 2 drops of 0.5%
proparacaine HC1 (Qphthaine Lot
___ were placed on the corneal
surface o f ______ eye(s) of each rabbit. Tech.
l______ L
Group fcjQ Dose:Q. I mL/ /vJAg f/On mL equivalent) Time: From
To i s :q %
^ Unflushed
fcjQ Flushed: k )& seconds after test material Instillation, the test eye of each animal was flushed with r J A _ mL of lukewarm tap water for k J A seconds / minutes. Time: From M A To /OA
lX Dose was administered using a 1-mL disposable syringe.
t jQ Dose was administered using a m A - uL glass syringe.
JdDose was weighed into and administered using a weighing paper.
jNJkDose was placed directly on the corneal surface of the right eye. The eyelids
were released Immediately without forced blinking or manipulation.
^ Dose was placed into the everted lower lid of the right eye. The upper and
lower lids were gently held together for one second and then released.
M Q Other: n J A __________________________________________________
-
Dosing Techn1cian/Date: ^ a c /
______
Time of 1-hour scores: k -.a q
Technician/Date: Q r /
______
NA Not applicable.
error a c
(E8/12-09-92)
Final data review by/Date: *77/ /
005778
__
HW I No.: *-402jQC>l-4-in> BODY WEIGHT / TREATMENT EFFECT RECORD
Test Material: "7~- - S _____________________________________
Species/Source Strain/Location: Rabbit/Hra:(NZW1SPF/MI
Date Animals Received:
12. - -C13
Initiated in Room Number: I
ANnuimmbaelr PUQftZFS
\
Group n !b. >f
Sex Time:IniStFi*al
PaiFnolRleoswpionngse
Animal BodyWeights (g)
I
Dosing Initiation Day7 Day 14 Day21 |
O (Of-jC-n* L |0
|3tod 1 m 12_
ks
3 1 9 9 9tSO j> -Q0>
> 3b2X 9l\to
Technician Date Scale Used
|Cm
ac
^23
Jira *
_________ A O
* Sodium fluorescein examination.
"t*
NEG Negative. POS Positive.
NA Not applicable.
V Vocalization. E Excessive pawing of treated eye. N None.
<xc 9-9H cSUrtHfoS
cxv
ac 3-3
s l , otvi 2 - n - q q - . IG=\ x-2-S-Rs-^
On the day of treatment, all animals were weighed and appeared normal before test
material administration. Technician/Date: a c
Surviving animals were designated for sacrifice and discard. Technician/Date: k'-ry A 3 - m
(E2/12-09-92)
Final data review by/Date: / 3 - 1 1~
005779
Hwi n o .: `-tozcyrLjnn
PRINARY EYE IRRITATION OBSERVATIONS
Test Material : Group: hJc\
"T- 5 S
----------------------------------------------- Test eye: Rioht
Observation Period:
1 IgAur-
Animal Number f"> j Location of Corneal
QO ooLesions
Q fv a Q"7Q5 \
tornea Iris
Opacity .
Area
\1
\
l __ 2 ____ 1
l1 I1
Redness tonjuncnvae ....
Chemosls
3 A
3
3 2
Discharge
3C
Qc
Sodium Fluorescein exam
/VJA f j A
Technician:
Q-C
Date: ________
Eye Irritation Score: oN-3_________ Calculated by/Date: a c / a-Q v-W _ Verified by/Date: ,7 / (
NA Not applicable. A Petite hemorrhaging. B Blanching.
C Clear discharge. D Purulent discharge. E Hair loss around the eye. F Possible necrotic areas. G Unable to visualize due to
severe conjunctival swelling. H No reaction to light. I Injected.
0 Corneal epithelial peeling. K Corneal epithelial pitting. L Corneal edema.
M Hypopyon. N Corneal neovascularization. P Pannus. R Unable to visualize due to
severe corneal opacity. S Granulation scar tissue. POS Positive stain retention. NEG Negative stain retention.
(E3/07-15-92)
Final data review by/Date:
005780
HW I No.: ^ 2 r j Q ^ 7 Q
Test Material: Group: hJf
PRIMARY EYE IRRITATION OBSERVATIONS
"7~- _________________________________________ _____
Test eye: Right
Animal Number Location of Corneal Lesions
Observation Period: 2J-4
Q@Q05Oo\QP>2> iK 2 a i-TQS
tornea
Opacity ....
Area
Iris
Redness vOnJUnCv1VaG
Chemosis
Discharge
Sodium Fluorescein exam
1 _ _ _ ! ____ 13
1* 2-& 11. Ie - 2 J>
--- kJC *
/ I )*
3 3e iOa
Technician: Date:
1C
Eye Irritation Score: Z u > .i
Calculated bv/Date:
/ X-XS-QU'
Verified bv/Date: J& S /
NA Not applicable. A Petite hemorrhaging. B B1anching. C Clear discharge. D Purulent discharge. E Hair loss around the eye. F Possible necrotic areas. G Unable to visualize due to
severe conjunctival swelling. H No reaction to light. I Injected.
J Corneal epithelial peeling. K Corneal epithelial pitting. L Corneal edema. H Hypopyon. N Corneal neovascularization. P Pannus. R Unable to visualize due to
severe corneal opacity. S Granulation scar tissue. POS Positive stain retention. NEG Negative stain retention.
(E3/07-15-92)
Final data review by/Date: ^
005781
--
Test Material: Group: r^\
HWI No.: J-/Q PRIMARY EYE IRRITATION OBSERVATIONS ~7~- S & Q Q ______________________________________________ _
Test eye: Right. Observation Period: _____ Y IP a 4>*,x>
NA Not applicable. A Petite hemorrhaging. B Blanching. C Clear discharge. D Purulent discharge. E Hair loss around the eye. F Possible necrotic areas. G Unable to visualize due to
severe conjunctival swelling. H No reaction to light. I Injected.
J Corneal epithelial peeling. K Corneal epithelial pitting. L Corneal edema.
M Hypopyon. N Corneal neovascularization.
P Pannus. R Unable to visualize due to
severe corneal opacity. S Granulation scar tissue. POS Positive stain retention. NEG Negative stain retention.
(E3/07-15-92)
Final data review by/Date:
005782
1^
HWI Mo.: * - / T > 2 y - f 7Q
PRIMARY EYE IRRITATION OBSERVATIONS
Test Material: Group: hJc\
T - 5 8 Q 8 _________________________________________ _____ Test eye: Riaht
Observation Period: ______7 ? A/ov/z.
Animal Number f i f Location of Corneal
Ow)QC5opLesions
Q & 2 & iP 2 Q
\
uornea
Opacity Area
o o
SKg //
//
Iris
/* o
Redness conjunctivae
/
J*
J
Chemosis
o
/
A
Discharge
/* o
Sodium Fluorescein exam A/&?
A/'a A r S * Z
Technician: _______.7 ? / Date: _______
Eye Irritation Score: .
__ _____
Calculated by/Date: *77/ / Verified by/Date: n r / 'k-s-Hy
NA Not applicable.
A Petite hemorrhaging. B Blanching. C Clear discharge.
D Purulent discharge. E Hair loss around the eye. F Possible necrotic areas. G Unable to visualize due to
severe conjunctival swelling.
H No reaction to light. I Injected.
J Corneal epithelial peeling. K Corneal epithelial pitting. L Corneal edema. M Hypopyon. N Corneal neovascularization.
P Pannus. R Unable to visualize due to
severe corneal opacity.
S Granulation scar tissue. POS Positive stain retention. NEG Negative stain retention.
(E3/07-15-92)
Final data review by/Date:
005783
--
HWI No.: - ^ 2 / Y V - / - 7 Q
PRIMARY EYE IRRITATION OBSERVATIONS
Test Material: Group: hJc\
"T"- -55& Q Q > ___________________________________________________ Test eye: Rioht
Observation Period: Q i s i-jn u
Animal Number Location of Corneal Lesions
PPOpooQ&Z& QPOQ Q-lQs \
uornea
Opacity O
11
Area I 1
Iris o o o
Redness conjunctivae
Chemosis
<o o
211
Discharge
oo
Sodium Fluorescein exam aJ A
Technician:
Date:
2 - - 2 J & - 9 t- L _
Eye Irritation Score: 7.3
Calculated bv/Date: //- / 2 .-2A - * W _ Verified by/Date: __ r\c 7 ^ - 3 - ^ _____
NA Not applicable. A Petite hemorrhaging. B B1anching. C Clear discharge. D Purulent discharge. E Hair loss around the eye. F Possible necrotic areas. G Unable to visualize due to
severe conjunctival swelling. H No reaction to light. I Injected.
J Corneal epithelial peeling.
K Corneal epithelial pitting. L Corneal edema. M Hypopyon. N Corneal neovascularization. P Pannus. R Unable to visualize due to
severe corneal opacity. S Granulation scar tissue. POS Positive stain retention. NEG Negative stain retention.
(E3/07-15-92)
Final data review by/Date: c x c / 3 -/>-^7
005784
HWI No.:
PRIMARY EYE IRRITATION OBSERVATIONS
Test Material: Group: hJd\
PQP>__________________________________________ _ Test eye: Right
Observation Period:
-?
Animal Number
Location of Corneal Lesions
OoOQOOQ?>2>
Q - 7Q 5 \
LOrnGa
Opacity Area
c> o
3-S%
o1 o1
Iris
o oO
Redness wOnjuncviVoG
Chemosis
o
0
1a
o1
Discharge O o n
Sodium Fluorescein exam MEG-
Pos s-%
\
Technician:
Or
Date: ________3 - 3 "T t
Eye Irritation Score:
Calculated by/Date: Verified by/Date:
S.3_________
c ic / 3-3 ^ /3-^.q<-f
NA Not applicable. A Petite hemorrhaging. B Blanching. C Clear discharge. D Purulent discharge. E Hair loss"around the eye. F Possible necrotic areas. G Unable to visualize due to
severe conjunctival swelling. H No reaction to light. I Injected.
J Corneal epithelial peeling. K Corneal epithelial pitting. L Corneal edema. M Hypopyon. N Corneal neovascularization. P Pannus. R Unable to visualize due to
severe corneal opacity. S Granulation scar tissue. POS Positive stain retention. NEG Negative stain retention.
(E3/07-15-92)
Final data review by/Date:
-9^--
00578S
HWI No. J - j c Z L C & O n a
PRIMARY EYE IRRITATION OBSERVATIONS
Test Material: Group: frJt)
'1~- *5 B Q Q
Test eye: Rioht
Animal Number Location of Corneal Lesions
Observation Period:
^ /<-f
OOOQO0f'j f.
NQ - 7 Q 5
Lornoa
Opacity Area
Iris
Redness uonjuncc1 vae
Chemosis
Discharge
Sodium Fluorescein exam
o
o
O
o
o
___Q ____
o o o
/vlcbi
o
o o o
o
Technician: Date:
Eye Irritation Score: o.o
Calculated bv/Date: Verified bv/Date:
a c /3-n-^H
NA Not applicable. A Petite hemorrhaging. B Blanching. C Clear discharge. D Purulent discharge. E Hair loss around the eye. F Possible necrotic areas. G Unable to visualize due to
severe conjunctival swelling. H No reaction to light. I Injected.
J Corneal epithelial peeling. K Corneal epithelial pitting. L Corneal edema. M Hypopyon. N Corneal neovascularization. P Pannus. R Unable to visualize due to
severe corneal opacity. S Granulation scar tissue. POS Positive stain retention. NEG Negative stain retention.
(E3/07-15-92)
Final data review by/Date: CXc /3--H-RH
005786
HWI No.: ^ O O ^ - 7 < Q
SCALE FOR SCORING OCULAR LESIONS (DRAIZE TECHNIQUE)
(1) Cornea
(A) Opacity - Degree of density (area most dense taken for reading)
No opacity.................................................................................... 0 Scattered or diffuse area, details of Iris clearly visible................................. 1 * Easily discernible translucent areas, details of iris slightly obscured................... 2*
Opalescent areas, no details of Iris visible, size of pupil barely discernible............3*
Opaque, iris invisible........ ...............................................................4*
(B) Area of Cornea Involved One-quarter (or less), but notzero.......................................................... 1 Greater than one-quarter, but less than half..... ................. ........................ 2 Greater than half, but less than three-quarters............................................. 3 Greater than three-quarters up to whole area................................................ 4
A x B x 5 Total Maximum * 80
(2) M s
(A) Values
Normal........................................................................................ 0 Folds above normal, congestion, swelling, circumcorneal injection (any or all of these or combination of any thereof) iris 1s still reacting to light (sluggish reaction is
positive) ..................................................................................... 1* No reaction to light, hemorrhage, gross destruction (any or all of these)................. 2*
Ax5
Total Maximum * 10
(3) Conjunctivae
(A) Redness (refers to palpebral and bulbar conjunctivas excluding cornea and iris) Vessels normal................................................................................0 Vessels definitely Injected above normal.................................................... 1 More diffuse, deeper crimson red, individual vessels not easily discernible.............. .2*
Diffuse beefy red.............................................................................3*
(B) Chemosis No swelling................................................................................... 0 Any swelling above normal (includes nictitatingmembrane)................................... 1 Obvious swelling with partial eversion of the lids.......................................... 2* Swelling with lids about half closed......................................................... 3* Swelling with lids about half closed to completely closed.................. ................ 4*
(C) Discharge
No discharge...................... ............................................................0 Any amount different from normal (does not Include small amounts observed in Inner canthus of normal animals)................................................................... 1 Discharge with moistening of the lids and hairs just adjacent to the lids................. 2 Discharge with moistening of the lids and hairs, and considerable area around the eye.....3
Score (A + B + C) x 2 Total Maximum = 20
The total score for the eye is the sum of all scores obtained for the cornea, iris, and conjunctivae.
* Indicates a positive effect. (FHSA Interpretation)
(E4/01-06-91)
005787
