Document OE9G0q1Y3Qqkd5NJMMqpYObNj
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SANITIZED
REPORT
EXPLORATORY 28-DAY ORAL TOXICITY STUDY
WITH
TELOMER ALCOHOL, TELOMER ACRYLATE.
PFHS AND PFOS (positive control)
BY DAILY GAVAGE
IN THE RAT
FOLLOWED BY A 14/28-DAY RECOVERY PERIOD
NOTOX Project 242933 NOTOX Substances 83484, 83493, 83502, 84456 and 84141
3M Test No. T-7023, T-7024, T-7022, T-7032, T-6295
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TELOMER ALCOHOL/TELOMER ACRYLATE/ REPORT APPROVAL
PFHS/PFOS
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NOTOX Project 242933 *
Study Director Ir. S.C.M. de Hoog
Management - Drs. W.J.A.M. Frleling
Date:
Q-p^'l 1 Q Q 0
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TELOMER ALCOHOL/TELOMER ACRYLATE/
CONTENTS
PFHS/PFOS
REPORT APPROVAL
SUMMARY
PREFACE .
STUDY PLAN
MATERIALS AND METHODS
Test Substances Test Substance Formulation Purpose and Rationale Guidelines Archiving Test System Allocation Animal Husbandry Treatment Recovery Observations
Mortality / Viability Clinical Signs Body Weights Food Consumption Water- Consumption Clinical Laboratory Investigations Haematology Clinical Biochemistry Urinalysis Pathology Necropsy Organ Weights Liver Sampling Hlstotechnology Histopathology Statistical Analysis
RESULTS
Observations Mortality Clinical Signs
.
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NOTOX Project 242933
*
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5
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8
9
9 10 11 11 11 12 12 13 13 13 14 14 14 14 14 14 14 15 16 17 17 17 18 18 18 19 19
20
20 20 20
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TELOMER ALCOHOL/TELOMER ACRYLATE/
PFHS/PFOS
NOTOX Project 242933
RESULTS (continued)
21
Body Weight Food Consumption Clinical Laboratory Investigations
Haematology Clinical Biochemistry
Pathology Macroscopic Examination Organ Weights Microscopic Examination
Discussion and Conclusion
21 21 22
22 23
24 24 24 25 25 .
FIGURES Body Weights Body Weight Gain Food Consumption Relative Food Consumption
27 28-29 30-31 32-33 34-35
TABLES - SUMMARY DATA
Clinical Signs Body Weights Body Weight Gain Food Consumption Relative Food Consumption Haematology Clinical Biochemistry Organ Weights-Organ/Body Weight Ratios Historical data Haematology / Clinical
Biochemistry
36 37-60 61-64 65-68 69-72 73-76 77-88 89-100 101-112
113-118
TABLES - INDIVIDUAL DATA
119
Mortality
120-125
Clinical Signs, Daily
126-152
Body Weights
153-160
Body Weights Gain
161-168
Food Consumption Relative Food Consumption
169-172 173-184
Key to Missing Values and Remarks Haematology /
Clinical Biochemistry
185
Haematology
186-221
Clinical Biochemistry
222-257
Macroscopic Findings
258-281
Organ Weights-Organ/Body Weight Ratios
282-305
APPENDIX 1 Pathology Report LAST PAGE OF REPORT
1-146 451
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TELOMER ALCOHOL/TELOMER ACRYLATE/
PFHS/PFOS
NOTOX Project 242933
SUMMARY
Exploratory 28-day oral toxicity study with Telomer Alcohol, Telomer Acrylate, PFHS and PFOS (positive control) by daily gavage in the rat followed by a
14/28-day recovery period.
Dose levels for this study, provided by the sponsor, were selected to be 0
(Vehicle Control), 3 (PFOS), 10 (PFHS) and 30 mg/kg body weight/day (Telomer
Alcohol, Telomer Acrylate
)..
The study was based on the following guidelines: - EEC Directive 96/54/EEC, B.7 Repeated Dose (28 days) Toxicity (oral), 1996. - OECD 407, Repeated Dose 28-day Oral Toxicity Study in Rodents, 1995
The test substance, formulated in 1% aqueous carboxymethyl cellulose, was administered dally for at least 28 days by oral gavage to SPF-bred Sprague Dawley rats. One vehicle control group and five treated groups were tested, each consisting of 8 males and 8 females. An extra 3 animals per sex per group were
allowed 14 days of recovery and another 3 animals per sex per group were allowed 28 days of recovery.
The following parameters were evaluated: clinical signs twice daily (treatment) or once dally (recovery); body weight twice weekly; food consumption weekly; clinical pathology after 4 weeks and at the end of both recovery periods;
macroscopy at termination; organ weights and histopathology on a selection of tissues.
RESULTS
Telomer Alcohol: (30 mg/kg/day)
1. Total protein, globulin and total cholesterol were decreased (/) after treatment. Total protein was still
decreased after 2 and 4 weeks recovery (<?).
2. Midzonal/centrilobular hypertrophy was observed in the
liver, and exocrine apoptosis/single cell necrosis 1n the pancreas (</*) after treatment and had partially or completely resolved after 4 weeks recovery.
Telomer Acrylate: (30 mg/kg/day)
1. Body weights were decreased at the end of treatment (?) 2. Total protein, globulin and total cholesterol were
decreased (<?) and albumin/globulin ratios were increased (cf) after treatment.
(30 mg/kg/day)
1. Total protein, globulin, aspartate aminotransferase activity (<?) and prothrombin time (?) were decreased and albumin/globulin ratios were Increased (rf) after treatment. Prothrombin time was still decreased after 2 weeks recovery (?).
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TELOMER ALCOHOL/TELOMER ACRYLATE/
PFHS/PFOS
NOTOX Project 242933
PFHS : (10 mg/kg/day)
PFOS: (3 mg/kg/day)
1. Total cholesterol (d1 and ?), total-protein, globulin, triglycerides, aspartate aminotransferase activity (c?) and prothrombin time (<f and ?) were decreased; albumin/globulin ratios and alkaline phosphatase activity were increased (<?) after treatment. Prothrombin time was still decreased after 2 weeks recovery (?).
2. Liver weights were increased after treatment (<?). 3. Mldzonal/centrilobular hypertrophy was observed in the
liver (cr) after treatment and had completely resolved
after 4 weeks recovery.
1. Body weights and food consumption were decreased during treatment and recovery (?).
2. Total protein, globulin, total cholesterol, triglycerides, aspartate aminotransferase activity (d1) and prothrombin time (?) were decreased; albumin/globulin ratios were increased (d*) after treatment.
3. Autopsy body weights were decreased (?). Liver weights were increased after treatment and recovery (<? and ?).
4. Midzonal/centrllobular hypertrophy was observed 1n the liver (r and ?) after treatment and remained in evidence among males after recovery.
From the results presented 1n this report 1t can be concluded that the positive control compound PFOS dosed at 3 mg/kg/day was indeed the compound with the most striking and persistent effects, mainly in the liver. Compounds with comparable but less severe effects as PFOS included PFHS (10 mg/kg/day) and TELOMER ALCOHOL (30 mg/kg/day). Despite slightly reduced body weights in females (TELOMER ACRYLATE only) and some slight changes in the blood after treatment, 30 mg/kg TELOMER ACRYLATE or
per day did not result in clear systemic toxicity and organ dysfunction at macroscopic or microscopic level.
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TELOMER ALCOHOL/TELOMER ACRYLATE/
PFHS/PFOS
NOTOX Project 242933
PREFACE Sponsor Study Monitor
Invoice Contact
Testing Facility Study Director Histopathology
3M Toxicology Services 3M Center, Bldg 220-2E-02 St. Paul, MN 55144-1000 USA .
Marvin T. Case, DVM, PhD 3M Toxicology Services 3M Center, Bldg 220-2E-02 St. Paul MN 55144-1000 Telephone (651)- 733-5180 Fax (651)- 733-1773
Barbara Nelson 3M Procurement Operations 3M Center, Bldg 224-1N-04 St. Paul, MN 55144-1000 Telephone (651)- 733-0766 Fax (651)- 733-1799
NOTOX B.V. Hambakenwetering 3 5231 DD 's-Hertogenbosch The Netherlands
Ir. S.C.M. de Hoog Dr. J. Th. Wilson
.
.
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TELOMER ALCOHOL/TELOMER ACRYLATE/
PFHS/PFOS
NOTOX Project 242933
STUDY PLAN
*
Start of Treatment
06 October 1998 (males) 08 October 1998 (females)
Start Recovery
04 November 1998 (males) 06 November 1998 (females)
Blood sampling
03 November 1998 (Main groups, males) 04 November 1998 (Main and Recovery groups 1 and 2, males) 05 November 1998 (Main groups, females)
06 November 1998 (Main and Recovery groups 1 and 2, females) 18 November 1998 (Recovery groups 1, males) 20 November 1998 (Recovery groups 1, females) 02 December 1998 (Recovery groups 2, males) 04 December' 1998 (Recovery groups 2, females)
Urine collection
predose day 7 day 7 day 14 day 14 end of treatment end of treatment end of recovery end of recovery end of recovery end of recovery
Necropsy
29-30 September 1998 (Recovery groups 1 and '2)
11-12 October 1998 (Recovery groups 1 and 2, males)
13-14 October 1998 (Recovery groups 1 and 2, females) 18-19 October 1998 (Recovery groups 1 and 2, males)
20-21 October 1998 (Recovery groups 1 and 2, females) 03-04 November 1998 (Recovery groups 1 and 2, males) 05-06 November 1998 (Recovery groups 1 and 2, females) 17-18 November 1998 (Recovery groups 1, males) 19-20 November 1998 (Recovery groups 1, females)
01-02 December 1998 (Recovery groups 2, males) 03-04 December 1998 (Recovery groups 2, females)
03 November 1998 (Main groups, males) 04 November 1998 (Main groups, males) 05 November 1998 (Main groups, females) 06 November 1998 (Main groups, females) 18 November 1998 (Recovery groups 1, males) 20 November 1998 (Recovery groups 1, females) 02 December 1998 (Recovery groups 2, males) 04 December 1998 (Recovery groups 2, females)
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TELOMER ALCOHOL/TELOMER ACRYLATE/
PFHS/PFOS
MATERIALS AND METHODS
NOTOX Project 242933
-
TEST SUBSTANCES
The sponsor is responsible for the completeness of all test substance data. All available information on characteristics of the test substance were taken into
account before the start of the study in order to determine if they conflict with/might affect the proceeding of the study.
NOTOX SUBSTANCE 83484
Identification Description Batch Purity Test substance storage Stability under storage conditions Expiry date
Specific Gravity Stability in vehicle
TELOMER ALCOHOL Pale to medium tan waxy solid T-7023 Not Indicated by sponsor; treated as 100% pure At room temperature in the dark
Stable 07 September 2000 1.7 1% aqueous carboxymethyl cellulose: not indicated
NOTOX SUBSTANCE 83493
Identification Description Batch Purity Test substance storage Stability under storage conditions Expiry date Density Stability in vehicle
NOTOX SUBSTANCE 83502
TELOMER ACRYLATE Yellow liquid T-7024
Not indicated by sponsor; treated as 100% pure At room temperature protected from light
Stable
.
07 September 2000
Approx. 1.6 g/cnr at 50 "C
1% aqueous carboxymethyl cellulose: not Indicated
Identification
Description
Batch
Purity
.
Test substance storage
Stability under storage
conditions
Expiry date
Stability in vehicle
White powder T-7022 Not indicated by sponsor; treated as 100% pure At room temperature in the dark
Stable 07 September 2000 1% aqueous carboxymethyl cellulose: not indicated
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PFHS/PFOS
NOTOX Project 242933
NOTOX SUBSTANCE 84456/B
Identification Description Batch Purity Test substance storage Stability under storage conditions Expiry date Stability in vehicle
NOTOX SUBSTANCE 84141
Identification Description Batch Purity Test substance storage Stability under storage conditions Expiry date " Stability in vehicle
*
PFHS Off-white crystalline solid T-7042 Not indicated by sponsor; treated as 100% pure At room temperature in the dark
Stable 25 September 2000 1% aqueous carboxymethyl cellulose: not indicated
PFOS White solid T-6295 Not indicated by sponsor; treated as 100% pure At room temperature in the dark
Stable 07 September 2000 1% aqueous carboxymethyl cellulose: not indicated
Vehicle Rationale for vehicle Stability in vehicle
1% aqueous carboxymethyl cellulose
Based on trial formulations performed at NOTOX Stability over 4 hours will be determined by the sponsor
TEST SUBSTANCE FORMULATION
Method
Formulations (w/w) were prepared daily within 4
hours prior to dosing. No adjustment was made for specific gravity of the test substance.
Storage conditions
At ambient temperature
No chemical analysis of dose preparations was performed.
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TELOMER ALCOHOL/TELOMER ACRYLATE/
PFHS/PFOS
NOTOX Project 242933
PURPOSE AND RATIONALE
*
The nature and purpose of this exploratory study was to determine and compare the relative oral toxicity in rats following oral (gastric gavage) doses administered daily over 28 days and identify developmental compounds of lesser toxicity for commercial development.
This study should provide part of a rational basis for toxicological risk assessment 1n man. The oral route was selected as it 1s a possible route of human exposure during manufacture, handling or use of the test substance.
GUIDELINES
The protocol was reviewed and agreed by the Article 14-functionary and the Ethical Committee of NOTOX as required by the Dutch Act on Animal Experimentation. The study procedures described in this report were based on the following guidelines:
1) EC Directive 96/54/EEC, Annex IV.D, replacing EEC Directive 67/548/EEC, Part B : Methods for the Determination of Toxicity and other Health Effects; B.7: 'Repeated Dose (28 days) Toxicity (oral)*. Official Journal of the European Communities No. L248, September 1996.
2) OECD Guideline for Testing of Chemicals, Guideline 407: `Repeated Dose 28-day Oral Toxicity Study in Rodents', Paris Cedex, 27 July 1995
The protocol, study procedures and the report were not inspected by the NOTOX Quality Assurance Unit.
ARCHIVING
NOTOX B.V., will archive the following data for at least 10 years: protocol, raw data, report and all specimens. No data will be withdrawn without the sponsor's written consent.
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PFHS/PFOS
NOTOX Project 242933
TEST SYSTEM
*
Test System
Age at Start of Treatment Number of animals Randomisation
Identification
Sprague Dawley rat Cr1:CD BR (outbred, SPF-Quality) Recognised by international guidelines as the recommended test system (e.g. EPA, FDA, OECD, EEC). Females were nulliparous and non-pregnant. Source : Charles River, Sulzfeld, Germany
Approximately 6 weeks.
84 males, 84 females
At least 5 days before study start, by computer generated random algorithm according to body weight, with all animals within 20% of the sex mean. A health inspection was performed prior to commencement of treatment to ensure that the animals were in a good state of health.
Earmark and tattoo
ALLOCATION
Group Identification
Test Substance
Number of animals Animal numbers
males females
males females
1 Main group 1 Recovery group 1 1 Recovery group 2 2 Main group 2 Recovery group 1 2 Recovery group 2 3 Main group 3 Recovery group 1 3 Recovery `group 2 4 Main group
4 Recovery group 1 4 Recovery group 2 5 Main group 5 Recovery group 1 5 Recovery group 2 6 Main group
6 Recovery group 1 6 Recovery group 2
Vehicle control
8
Vehicle control
3
Vehicle control
3
Telomer Alcohol
8
Telomer Alcohol
3
Telomer Alcohol
3
Telomer Acrylate
8
Telomer Acrylate
3
Telomer Acrylate
3
8
3
.3
PFHS
8
PFHS
3
PFHS
3
PFOS (positive control) 8
PFOS (positive control) 3
PFOS (positive control) 3
8 3 3 8 3 3 8 3 3 8 3 3 8 3 3 8 3
3
1- 8
9-11 13-15 17-24 25-27
29-31 33-40 41-43 45-47 49-56
57-59 61-63 65-72 73-75 77-79 81-88
89-91 93-95
97-104 105-107
109-111 113-120 121-123 125-127 129-136 137-139 141-143 145-152 153-155
157-159 161-168 169-171 173-175 177-184 185-187
189-191
Test substances Telomer Alcohol (substance 83484), Telomer Acrylate (substance
83493) and
(substance 83502) were tested at a dose level of 30 mg/kg body
weight/day.
PFHS (substance 84456) was tested at a dose level of 10 mg/kg body weight/day. PFOS (substance 84141) was tested at a dose level of 3 mg/kg body weight/day.
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TELOMER ALCOHOL/TELOMER ACRYLATE/
PFHS/PFOS
NOTOX Project 242933
ANIMAL HUSBANDRY
*
Conditions Air-conditioned room with approximately 15 air changes per hour and the environment controlled with optimal conditions considered as being a temperature of 21C and a relative humidity of 50%. Lighting was 12 hours artificial fluorescent light and 12 hours dark per day. Deviations from these optimal conditions occurred, but were considered not to have affected study integrity.
Accommodation Group housing of 3-4 animals per sex per cage in stainless steel suspended cages with wire mesh floors. Acclimatisation period was at least 5 days before start of treatment under laboratory conditions.
Diet Free access to standard pelleted laboratory animal diet (from Carfil Quality BVBA, Oud-Turnhout, Belgium). Each batch is analysed for nutrients and contaminants are analysed on a regular basis. Results are examined and archived.
Water Free access to tap-water. Certificates of analysis (performed quarterly) were examined and archived.
TREATMENT
Method Frequency Dose volume Dose level
Oral gavage, using a stainless steel stomach tube. Formulations were placed on a magnetic stirrer during dosing.
Once daily for at least 28 days, approximately the same time each day, 7 days per week. Main group animals were dosed up to the day prior to necropsy
5 ml/kg body weight. Actual dose volumes were calculated weekly according to the latest body weight.
Group 1: 0 mg/kg b.w./day (Vehicle Control) Group 2: 30 mg/kg.,b.w./day (Telomer Alcohol) Group 3: 30 mg/kg b.w./day (Telomer Acrylate) Group 4: 30 mg/kg b.w./day ( ) Group 5: 10 mg/kg b.w./day (PFHS) Group 6: 3 mg/kg b.w./day (PFOS, positive control)
RECOVERY
Duration
Recovery group 1: at least 14 days Recovery group 2: at least 28 days
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PFHS/PFOS
NOTOX Project 242933
OBSERVATIONS
.
Mortality / Viability
Twice.daily.
Clinical signs
!
During the treatment period detailed clinical observations were made twice daily in all animals: once immediately after dosing and once
approximately 4 hours after dosing. During the recovery period only one observation per day was performed. The time of onset, degree and duration were recorded.
All symptoms were recorded and graded according to fixed scales:
Maximum grade 4: grading slight (1) to very severe (4). Maximum grade 3: grading slight (1) to severe (3). Maximum grade 1: presence is scored (1).
Body weights
Treatment period : on days 1, 5, 8, 12, 15, 19, 22, 25 and 28.
Recovery period : on days 1, 5, 8, 12, 14, 19, 22, 25 and 28.
Food consumption
Weekly.
.
Water consumption
Subjective appraisal was maintained during the study, but no quantitative investigation introduced as no effect was suspected.
CLINICAL LABORATORY INVESTIGATIONS
Blood samples were collected under light ether anaesthesia, between 7.30 and
9.30 a.m. immediately prior to post mortem examination for Main and Recovery
group animals, and for Recovery group animals also at the end of the treatment
period. The animals were fasted overnight (with a maximum of 20 hours) before
blood sampling, but water was provided. Blood samples were drawn from the retro-
orbital sinus of all Main and Recovery rats/sex/group and collected Into tubes
prepared with EDTA for haematological parameters (0.5 ml), with citrate for
clotting tests (1.0 ml) and untreated tubes for clinical biochemistry parameters
(>2.0 ml). From animal numbers 17-20, 33-36, 49-52, 65-68, 81-83, 113, 115, 131
and 132 a second EDTA-sample was collected after 4 weeks, due to inexplicable
clotting of the first sample.
.
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TELOMER ALCOHOL/TELOMER ACRYLATE/
PFHS/PFOS
NOTOX Project 242933
HAEMATOLOGY
The following haematology parameters were determined from blood prepared with EDTA as an anti-coagulant, using the instruments listed:
Parameter/Abbreviation
Unit
Instrumentation
Erythrocyte count/RBC
T/l
Haemoglobin/HB
mmo1/1
Haematocrit/HCT
1/1
Mean corpuscular volume/MCV fl
Mean corpuscular haemoglobin/MCH
fmol
Mean corpuscular haemoglobin mmol/I concentrtion/MCHC
Platelet count
G/l
Red cell distribution width/RDW
%
Total leucocyte count/WBC G/l
Differential leucocyte
count/SEG (Neutrophils) EO (Eosinophils), BASO (Basophils), LYMPH (Lymphocytes), MONO (Monocytes)
l(rel)
Sysmex K-1000 Sysmex K-1000 Sysmex K-1000 Sysmex K-IOOO Sysmex K-1000
Sysmex K-1000
Sysmex K-IOOO
Sysmex K-1000 Sysmex K-IOOO Manual (Microscope)
The following haematology parameters were determined from blood prepared with
citrate as an anti-coagulant, using the instruments listed:
Parameter/Abbrev1at ion
Unit
Instrumentation/Method
Prothrombin time/PT
Partial thromboplastin time/PTT
sec sec
Sysmex CA-5000 Sysmex CA-5000
'
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PFHS/PFOS
NOTOX Project 242933
CLINICAL BIOCHEMISTRY
The following clinical biochemistry parameters were determined from serum samples using the instruments listed:
Parameter/Abbreviai ion
Unit
Instrumentation
Alanine aminotransferase/ (ALAT/GPT)
pkat/1
Epos selective analyser 5060 (Eppendorf)
Aspartate aminotransferase/ pkat/1 (ASAT/GOT)
Epos selective analyser 5060
(Eppendorf)
.
Bilirubin, total/ BILI T.
pmo1/1
Epos selective analyser 5060 (Eppendorf)
Cholesterol, total/ CHOLEST. T.
mmol/1
Epos selective analyser 5060 (Eppendorf)
Triglycerides/ TRIGL.
mmol/1
Epos selective analyser 5060 (Eppendorf)
Creatinine
pmo1/1
Epos selective analyser 5060 (Eppendorf)
Glucose
mmo1/1
Epos selective analyser 5060 (Eppendorf)
Urea
mmol/1
Epos selective analyser 5060 (Eppendorf)
Protein, total/ PROTEIN T.
9/1 Epos selective analyser 5060 (Eppendorf)
Protein, albumin/ ALBUMIN
Protein, globulin/ GLOBULIN
o/i 8/1
Epos selective analyser 5060 (Eppendorf)
Calculation [PROTEIN T.-ALBUMIN]
Albumin Globulin ratio A/G RATIO
1
Calculation [ALBUMIN/GLOBULIN]
Alkaline phosphatase/ ALP
pkat/1
Epos selective analyser 5060 (Eppendorf)
Sodium
mmo1/1
EFIX 5055 Flame Photometer Eppendorf
Potassium
mmo1/1
EFIX 5055 Flame Photometer Eppendorf
Chloride
mmo1/1
Jenway PCLM3
Calcium
mmo1/1
EFIX 5055 Flame Photometer Eppendorf
Phosphorus/ INORG. PHOSPH
mmo1/1
' Epos selective analyser 5060
(Eppendorf)
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TELOMER ALCOHOL/TELOMER ACRYLATE/
PFHS/PFOS
NOTOX Project 242933
URINALYSIS (Recovery group animals only)
*
Urine samples were collected overnight (approximately 16 hrs) from all animals assigned to the Recovery groups according to the following schedule:
- predose - during the treatment period on days 7, 14 and at the end of treatment - at the end of the recovery period, prior to scheduled necropsy.
During the sampling period animals were deprived of food but water was provided. Urine was collected into a specimen vial, using a metabolism cage. After collection the samples were frozen and stored at -20oC at NOTOX until further analysis.
PATHOLOGY
NECROPSY
All animals surviving to the end of the observation period were deeply anaesthetised using ether vapour and subsequently exsanguinated. All animals
assigned to the study were necropsied and descriptions of all macroscopic abnormalities recorded.
Prior to exsangu1nat1on a blood sample (1-2 ml) for serum collection was taken from the aorta. Serum samples were stored at -208C at NOTOX until further analysis. Inadvertently, no serum sample was collected from all Main males and females from groups 1, 2, 3 and 6 (animal numbers 97-100, 113-116, 129-132 and 177-180). Instead, reserve samples intended for clinical biochemistry might be used for possible further analysis.
Samples of the following tissues and organs were collected from all animals at necropsy and fixed in neutral phosphate buffered 4% formaldehyde solution:
Identification marks Adrenal glands
Aorta
Brain
Caecum
Colon
Duodenum
Epididymides
Eyes with optic nerve and Harderian gland
Female mammary gland area
Heart
Ileum
Jejunum
Kidneys
Liver
.
Lung, infused with formalin
Lymph nodes - mandibular, mesenteric
Oesophagus
Ovaries
Pancreas
.
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TELOMER ALCOHOL/TELOMER ACRYLATE/
PFHS/PFOS
NOTOX Project 242933
Peyer's patches (jejunum, ileum) if detectable
Pituitary gland
Prostate gland
.
Rectum Salivary glands - mandibular
Sciatic nerve
Seminal vesicles Spinal cord -cervical, midthoracic, lumbar
Spleen Sternum with bone marrow
Stomach
Testes
Thymus
Thyroid including parathyroid
Trachea
Urinary bladder
Uterus
All gross lesions
ORGAN WEIGHTS
The following organ weights (and terminal body weight) were recorded from the surviving animals on the scheduled day of necropsy:
Adrenal glands Brain Heart Kidneys Liver Ovaries Spleen Testes
Both absolute and relative (% body weight) organ weight data are included 1n the study report.
LIVER SAMPLING
From all animals surviving until the scheduled necropsy at least 1 gram of the left lateral liver lobe was frozen in isopentane^. Liver samples were stored at -20'C at NOTOX and on 01 February 1999 dispatched on dry ice to the sponsor.
Analysis is performed under the responsibility of the sponsor. Results may be reported by the sponsor in a separate document.
HISTOTECHNOLOGY
All underlined organ and tissue samples (see under Necropsy) from all Main and Recovery group animals were processed, embedded and cut at a thickness of 2-4 micrometers and stained with haematoxylin and eosin.
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PFHS/PFOS
NOTOX Project 242933
HISTOPATHOLOGY
.
.
Slides of all underlined organs and tissues (see under Necropsy) collected at the scheduled sacrifice from all Main group animals of all dose groups as well as from all animals of all dose groups which died spontaneously, and all gross lesions of all animals (all dose groups) were examined by a pathologist. Based on treatment related morphologic changes, the following organs were also examined from all rats of Recovery groups 1 and 2 :
- liver from males of the Vehicle control, Telomer Alcohol, PFHS and PFOS groups;
- liver from females of the Vehicle Control and PFOS groups; - pancreas from males of the Vehicle Control and Telomer Alcohol groups.
All abnormalities were described and included in the report.
STATISTICAL ANALYSIS
The following statistical methods were used to analyse the data:
Univariate one-way analysis of variance was used to assess the significance of intergroup differences. .
If the variables could be assumed to follow a normal distribution, the Dunnett test (many-to-one t-test) based on a pooled variance estimate was applied for the comparison of the treated groups and the control groups for each sex.
The Steel-test (many-to-one rank test) was applied instead of the Dunnett-test when the data could not be assumed to follow a normal distribution.
All tests were two-sided and in all cases p < 0.05 was accepted as the lowest level of significance.
Group means were calculated for continuous data and medians were calculated for discrete data (scores) in the summary tables.
Test statistics were calculated on the basis of exact values for means and pooled variances. Individual values, means and standard deviations may have been rounded off before printing. Therefore, two groups may display the same printed means for a given parameter, yet display different test statistics values.
References:
- C.W. Dunnett
.
A Multiple Comparison Procedure for Comparing Several Treatments with a Control,
J. Amer. Stat. Assoc. 50, 1096-1121 (1955).
- R.G. Miller Simultaneous Statistical Inference, Springer Verlag, New York (1981).
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RESULTS
PFHS/PFOS
NOTOX Project 242933
-
OBSERVATIONS
MORTALITY
.
No treatment-related mortality occurred during the study period.
One female receiving TELOMER ACRYLATE died following blood sampling, prior to necropsy. This was considered to be an accidental death.
CLINICAL SIGNS
TELOMER ALCOHOL Rales were noted in one male immediately and 4 hours after dosing. This was observed at the end of the treatment period until the third week of recovery.
TELOMER ACRYLATE A hunched posture was observed in two Main group females in weeks 2 and 3 of the treatment period and in two Recovery group females at the end of treatment and during the first week(s) of recovery. Piloerection was also observed in the one female during the first week of recovery. In general the findings were observed immediately and 4 hours after dosing.
A hunched posture was observed immediately and 4 hours after dosing in one Main group female from week 3 until the end of the treatment period.
PFHS A hunched posture was observed immediately and 4 hours after dosing in one male at the end of treatment until the second day of recovery. A similar finding was seen 4 hours after dosing in one Main group female on one occasion at the end of treatment.
PFOS A hunched posture was observed in two Main group females in weeks 3 and 4 of treatment. A swelling on the abdominal skin was observed in one Main group male at the end of the treatment period.
Other findings such as alopecia, salivation, a missing tail apex, red discolouration of the skin, wounds, scabs, scales, fissures with bleeding on the paws, swelling of the ear and eye abnormalities were noted among the treated and/or control animals. These findings were considered to be of no toxicological significance as incidences remained within the range of biological variation for rats of this age and strain or could be attributed to the blood sampling procedure.
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TELOMER ALCOHOL/TELOMER ACRYLATE/
PFHS/PFOS
NOTOX Project 242933
BODY WEIGHT
.
TELOMER ALCOHOL Body weight gain of males was decreased at the end of the recovery period when
compared to the corresponding control values. Absolute body weights of these animals remained however within the range of control values.
TELOMER ACRYLATE
.
Body weights of females were slightly decreased when compared to control values
at the end of the treatment period.
PFHS
Body weight gain of males was decreased at the end of the recovery period. Absolute body weights of these males were also below control values, but did not differ with statistical significance.
PFOS
.
Body weights and body weight gain of females were decreased with statistical
significance from week 2 of treatment until the end of the recovery period.
Statistical significance was achieved in the majority of cases, mainly for
absolute body weights. Among males, body weight gain was reduced with
statistical significance in weeks 3 and 4 of the recovery period. Absolute body
weights of these males were also below control values, but did not differ with
statistical significance.
Body weights and body weight gain of animals receiving
remained in the same
range as controls over the treatment and recovery period.
FOOD CONSUMPTION
PFHS
Absolute food consumption among males was slightly reduced at the end of the 4week recovery period. However, after correction for body weight this effect was not observed anymore.
PFOS
Absolute food consumption of females was reduced from week 3 of treatment until
the end of recovery. This effect was less apparent after allowance for body
weight.
.
Among males, absolute food consumption was slightly reduced at the end of the 4-
week recovery period, but relative food consumption was within control values.
There were no differences in food consumption before or after allowance for body
weight between animals receiving TELOMER ALCOHOL, TELOMER ACRYLATE or
and
control animals.
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TELOMER ALCOHOL/TELOMER ACRYLATE/
PFHS/PFOS
NOTOX Project 242933
CLINICAL LABORATORY INVESTIGATIONS
HAEMATOLOGY
TELOMER ALCOHOL After 4 weeks, red blood cell count, haemoglobin and haematocrit values were decreased 1n females. After 2 weeks recovery, prothrombin time was decreased in females. After 4 weeks recovery, red cell distribution width was decreased in males.
TELOMER ACRYLATE After 4 weeks, total red blood cell count was slightly decreased in males. This decrease was no apparent anymore after the 2- and 4-week drug-free recovery periods.
After 4 weeks, differential leucocyte count revealed decreased relative values of neutrophilic granulocytes and increased relative lymphocytes values in males. Partial thromboplastin time was increased in males of this dose group. Prothrombin time was decreased in females. After 2 weeks recovery, prothrombin time was still decreased in females. After 4 weeks recovery, red cell distribution width was decreased in males.
PFHS After 4 weeks, total red blood cell count, haemoglobin, haematocrit, total number of platelets and prothrombin time were decreased in males. Total red blood cell count, haematocrit and prothrombin time were decreased in females. After 2 weeks recovery, prothrombin time and total platelet numbers were decreased 1n females. After 4 weeks recovery, no statistically significant differences when compared to controls were observed.
PFOS After 4 weeks, mean corpuscular haemoglobin was increased in males and haematocrit and prothrombin time were decreased in females. After the 2- and 4-week drug-free recovery periods no statistically significant differences were observed.
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TELOMER ALCOHOL/TELOMER ACRYLATE/
PFHS/PFOS
NOTOX Project 242933
CLINICAL BIOCHEMISTRY
.
TELOMER ALCOHOL
'
After 4 weeks, total cholesterol, total protein and globulin values were
decreased in males.
After 2 weeks recovery, total protein was still decreased 1n males.
After 4 weeks recovery, total cholesterol, total protein, potassium and calcium
values were decreased in males.
.
TELOMER ACRYLATE After 4 weeks, total cholesterol, total protein and globulin were decreased and albumin/globulin ratios were increased in males. After 2 weeks recovery these differences were not apparent anymore. After 4 weeks recovery, total protein, potassium and calcium values were decreased in males.
After 4 weeks, aspartate aminotransferase activity, total protein, globulin and potassium values were decreased and albumin/globulin ratios were increased in males.
After 2 weeks recovery, glucose values were increased and sodium values were decreased in males. Urea was increased in females. After 4 weeks recovery, total protein, inorganic phosphate, potassium and calcium values were decreased in males.
PFHS After 4 weeks, aspartate aminotransferase activity, total cholesterol, triglycerides, total protein and globulin values were decreased and albumin/globulin ratios, alkaline phosphatase activity and chloride were increased in males. Total cholesterol was decreased and chloride values were increased in females. After 2 weeks recovery, no statistically significant differences were observed anymore.
After 4 weeks recovery, total cholesterol, glucose and potassium values in males were below control values with statistical significance.
pfos
.
After 4 weeks, aspartate aminotransferase activity, total cholesterol,
triglycerides, total protein and globulin values were decreased and
albumin/globulin ratios and chloride were Increased in males. Urea and chloride
values were increased in females.
After 2 weeks recovery no such findings were observed.
After 4 weeks recovery, aspartate aminotransferase activity, total bilirubin,
total-protein, potassium and' calcium values were decreased and chloride was
increased in males.
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TELOMER ALCOHOL/TELOMER ACRYLATE/
PFHS/PFOS
NOTOX Project 242933
PATHOLOGY
'
.
MACROSCOPIC EXAMINATION
-
Macroscopic observations at necropsy did not reveal any alterations that were considered to have arisen as a result of treatment.
The few macroscopic findings recorded at all sacrifices were unremarkable and did not distinguish treated rats from the vehicle control group.
ORGAN WEIGHTS
PFHS After 4 weeks, liver weights and Hver:body weight ratios were increased in males.
After 2 and 4 weeks recovery, this effect had disappeared.
Increased brain:body weight and testes:body weight ratios observed after the 4-
week recovery period is attributed to the slightly low autopsy body weights of these animals.
PFOS
After 4 weeks, autopsy body weights were decreased 1n females. Liver weights and liver:body weight ratios were increased in males and females. Kidney:body weight ratios were increased in females.
After 2 weeks recovery, liver weights and liver:body weight ratios were still increased in males. After 4 weeks recovery, liver:body weight ratios were increased in males and females and kidney:body weight ratios were increased 1n females.
Other changes noted 1n PFOS treated animals included decreased absolute heart
weights and increased brain:body weight ratios in females after 4 weeks of treatment and 2 weeks recovery. In addition, absolute spleen weights were increased in males after 2 weeks recovery and testes:body weight ratios were
increased after 4 weeks recovery. All these changes were likely to reflect slightly low or high body weights at autopsy.
Organ weights and organ:body weight ratios of animals treated with TELOMER
ALCOHOL, TELOMER ACRYLATE or
were considered to be similar to those of
control animals.
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TELOMER ALCOHOL/TELOMER ACRYLATE/
PFHS/PFOS
NOTOX Project 242933
MICROSCOPIC EXAMINATION (see Appendix 1)
.
TELOMER ALCOHOL After 4 weeks, midzonal/centrllobular hypertrophy was seen at a minimal or slight degree in six males. In the pancreas a slight increase in the incidence of minimal or slight degrees of exocrine apoptosis/single cell necrosis was recorded in six males. After 2 and 4 weeks recovery, the liver changes had partially or completely resolved and the pancreas findings returned to the level of the vehicle control group.
PFHS After 4 weeks, midzonal/centrilobular hypertrophy was seen at slight to moderate degrees in all males. After 2 weeks recovery, this finding had partially resolved. After 4 weeks recovery, this finding had completely resolved. .
PFOS After 4 weeks, midzonal/centrllobular hypertrophy was seen at minimal to moderate degrees in all males and females. After 2 and 4 weeks recovery, this finding had partially or completely resolved in females, however remained in evidence in males.
The remainder of the microscopic findings recorded after treatment and recovery were within the range of background pathology encountered in Sprague Dawley rats of this age ad strain and occurred at similar incidences and severity in both control and treated rats.
DISCUSSION AND CONCLUSION
Following daily oral administrations of TELOMER ALCOHOL, TELOMER ACRYLATE, PFHS or PFOS to male and female Sprague Dawley rats for at least 28 days, treatment-related changes were most pronounced in the TELOMER ALCOHOL, PFHS and PFOS groups.
Hepatocellular hypertrophy 1n the liver together with higher liver weights was seen in animals receiving PFHS and PFOS. In the TELOMER ALCOHOL group the hepatic hypertrophy was not accompanied by increased liver weights. This liver enlargement is most likely related to the higher enzymatic activity in metabolising a xenobiotic compound. Among males receiving PFOS this effect was rather persistent since it was still observed at the end of the 4-week drugfree period.
Minimal to slight exocrine apoptosis/single cell necrosis was recorded in the pancreas of males receiving TELOMER ALCOHOL for 4 weeks and had resolved after the recovery period.
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TELOMER ALCOHOL/TELOMER ACRYLATE/
PFHS/PFOS
NOTOX Project 242933
Investigations of the blood showed that various parameters were slightly reduced
in one or both sexes after treatment. The most characteristic decreases were:
total protein and globulin in all dose groups;
- total cholesterol in all dose groups except for the
group;
* triglycerides in the PFHS and PFOS groups;
- aspartate aminotransferase activity and prothrombin time in the
,
PFHS and PFOS groups.
In the majority of cases these changes were not observed anymore after a 2-week drug-free period. A possible explanation for these changes could be liver damage as the liver is involved in the synthesis and metabolism of these compounds.
However, the only microscopic change noted 1n the liver was hepatocellular hypertrophy in the TELOMER ALCOHOL, PFHS and PFOS groups only. Moreover, it should be noted that the majority of values remained within the range of values that can be expected for animals of this age and strain. Changes in clinical biochemistry parameters noted after 4 weeks of recovery but not after 2 weeks were considered to have occurred by chance. At this time point in the study it seems very unlikely that they represent an effect of treatment.
Fluctuations in electrolyte levels were seen at all time points in all dose groups. Moreover, urea levels and kidney weights were increased after treatment in females receiving PFOS. However, in the absence of evidence of renal dysfunction at microscopic level these changes are considered to be of little toxicological relevance.
Haematological examination revealed slightly decreased red blood cell counts, haemoglobin and/or haematocrit values in animals treated with TELOMER ALCOHOL, TELOMER ACRYLATE, PFHS and PFOS. However, since the effects were slight in nature and no further evidence of an anaemic response was obtained at
macroscopic or microscopic level, the toxicological relevance of these findings is doubted. Other haematological changes noted were not supported by corresponding signs of toxicity and again the toxicological impact is considered to be of little relevance also because the values were within the range of historical data.
An effect on body weight and food consumption was most pronounced among females
receiving PFOS. This effect lasted until the end of the recovery period. Body weights of females receiving TELOMER ACRYLATE were slightly reduced at the end of treatment. Other effects on body weight gain and food consumption were mainly seen at the end of the recovery period and therefore no toxicological relevance is attached to these findings.
Clinical signs such as a hunched posture, piloerection and/or rales were observed incidentally in all five dose groups in a few animals only. At this low incidence and the severity observed these symptoms are considered not to represent a clear indication of toxicity.
From the results presented in this report it can be concluded that the positive control compound PFOS dosed at 3 mg/kg/day was indeed the compound with the most striking and persistent effects, mainly in the Uver. Compounds with comparable but less severe effects as PFOS included PFHS (10 mg/kg/day) and TELOMER ALCOHOL (30 mg/kg/day). Despite slightly reduced body weights in females (TELOMER ACRYLATE only) and
some slight changes in the blood after treatment, 30 mg/kg TELOMER ACRYLATE or per day did not result in clear systemic toxicity and organ dysfunction at
macroscopic or microscopic level.
- Page 26 -
TELOMER ALCOHOL/TELOMER ACRYLATE/
PFHS/PFOS
BACK TO MAIN
NOTOX Project 242933
FIGURES
27
NOTOX Project 242933
BODY WEIGHTS
MALES
GRAM
BACK TO MAIN
BW-SPLT - 1 08-DEC-98
- GROUP 1 (VEHICLE CONTROL) - GROUP 2 (TELOMERALCOHOL) - GROUP 3 (TELOMERACRYLATE) - GROUP 4 - GROUP 5 (PFHS) - GROUP (PFOS)
28
NOTOX Project 242933
BODY WEIGHTS
FEMALES
GRAM
BACK TO MAIN
BW-SPLT - 2 08-DEC-98
-------------- GROUP 1 (VEHICLE CONTROL) ............. GROUP 2 (TELOMER ALCOHOL) --------------- GROUP 3 (TELOMER ACRYLATE) -------------- GROUP 4 ------------- GROUP 5 (PFHS)
-------------- GROUP 6 (PFOS)
29
NOTOX Project 242933
BODY WEIGHT GAIN
MALES
X
BACK TO MAIN
BWG-SPLT - 1 08-DEC-98
-------------- GROUP 1 (VEHICLE CONTROL) .............. GROUP 2 (TELOMER ALCOHOL) --------------- GROUP 3 (TELOMER ACRYLATE) -------------- GROUP 4 ------------- GROUP 5 (PFHS)
-------------- GROUP 6 (PFOS)
30
NOTOX Project 242933
BODY WEIGHT GAIN
FEMALES
BACK TO MAIN
BWG-SPLT - 2 08-DEC-98
-------------- GROUP 1 (VEHICLE CONTROL) .............. GROUP 2 (TELOMER ALCOHOL) --------------- GROUP 3 (TELOMER ACRYLATE) -------------- GROUP 4 ------------- GROUP 5 (PFHS)
-------------- GROUP 6 (PFOS)
31
NOTOX Project 242933
FOOD CONSUMPTION
MALES
G/ANIMAL/DAY
BACK TO MAIN
FC-SPLT - 1 01-MAR-99
----------:---- GROUP 1 (VEHICLE CONTROL) .............. GROUP 2 (TELOMER ALCOHOL)
--------------- GROUP 3 (TELOMER ACRYLATE) -------------- GROUP 4
------------- GROUP 5 (PFHS)
-------------- GROUP 6 (PFOS)
NOTOX Project 242933
FOOD CONSUMPTION
FEMALES
G/ANIMAL/DAY
BACK TO MAIN
FC-SPLT - 2 01-MAR-99
-------------- GROUP 1 (VEHICLE CONTROL) ............. GROUP 2 (TELOMER ALCOHOL) --------------- GROUP 3 (TELOMER ACRYLATE) --------------- GROUP 4 ------------- GROUP 5 (PFHS)
-------------- GROUP 6 (PFOS)
33
NOTOX Project 242933
RELATIVE FOOD CONSUMPTION
MALES
G/KG BODY VEIGHT/DAY
BACK TO MAIN
RFC-SPLT - 1 01-MAR-99
-------------- GROUP 1 (VEHICLE CONTROL) .............. GROUP 2 (TELOMER ALCOHOL) --------------- GROUP 3 (TELOMER ACRYLATE) -------------- GROUP 4 ------------- GROUP 5 (PFHS)
-------------- GROUP 6 (PFOS)
34
NOTOX Project 242933
RELATIVE FOOD CONSUMPTION
FEMALES
G/KG BODY VEIGHT/DAY
BACK TO MAIN
RFC-SPLT - 2 01-MAR-99
-------------- GROUP 1 (VEHICLE CONTROL)
.............. GROUP 2 (TELOMER ALCOHOL)
--------------- GROUP 3 (TELOMER ACRYLATE)
-------------- GROUP 4
--------
GROUP 5 (PFHS)
-------------- GROUP 6 (PFOS)
TELOMER ALCOHOL/TELOMER ACRYLATE/
PFHS/PFOS
BACK TO MAIN
NOTOX 242933
TABLES - SUMMARY DATA
36
BACK TO MAIN
NOTOX Project 242933
SYM-SUM - 1 1 7 - M A R - 99
CLINICAL SIGNS (SUMMARY)
MALES GROUP 1 (VEHICLE CONTROL)
*
SIGN (MAX.GRADE)
TREATMENT WEEKS: 1........... 2 ............3 ............4............ 5. DAYS: 1.2.3.4.5.6.7.1.2.3.4.5.6.7.1.2.3.4.5.6.7.1.2.3.4.5.6.7.1. TIME: ABABABABABABABABABABABABABABABABABABABABABABABABABABABABAB
SKIN / FOR / PLUMAGE
ALOPECIA (3) (BACK)
ALOPECIA (3) (NECK)
ALOPECIA (3) (ABDOMEN)
ALOPECIA (3) (CHEST)
.
ALOPECIA (3) (FORELEG LEFT)
ALOPECIA (3) (HINDLEG LEFT)
ALOPECIA (3) (HINDLEG RIGHT)
SCABS (3) (NECK)
VARIOUS
BULGING EVE (1)
(EYE RIGHT)
.
DARK (3) (EYE RIGHT)
G: ........... 1111111111........... %: ........... 1111111111........... G: ..... 1111111111111111111........ %: ..... 1111111111111111111........ G: 111111111111111111111111111111111111.. %: 111111111111111111111111111111111111.. G: ..11............................. .
%: ..11.......................................
G: . . . 1222222222221111111111111111111111111. . . %: . . . 1111111111111111111111111111111111111. . . G: .....111111111111133illuminili..... %: ....11111111111111111111111111111...... G: ............ 1111111111111111111111111111111111. . . %: ............ 1111111111111111111111111111111111. . . G; ....................................111111111111111111111111111 %: ....................................111111111111111111111111111
G: %: G: %:
G: Median value of the highest individual grades % : Percent of affected animals (0 = less than 5%, 1 = between 5% and 15%,.... A = more than 95%) Time after treatment (H.Mi) A: 0.00 B: 4.00
NOTOX Project 242933
CLINICAL SIGNS, DAILY (SUMMARY)
MALES GROUP 1 (VEHICLE CONTROL)
SIGN (MAX.GRADE) LOCATION
RECOVERY WEEKS: 1 ..... 2 ..... 3 ..... 4 ..... 5.
SKIN / FUR / PLUMAGE
ALOPECIA (3) (CHEST)
VARIOUS
BULGING EYE (1) (EYE RIGHT)
DARK (3) (EYE RIGHT)
G: .....12222222 %: ..... 22222222
G: 11 %: 12 G: 11 %: 12
BACK TO MAIN
SYM-SUM - i 2 5 - M A R - 99
G: Median value of the highest individual daily grades %: Percent of affected animals (0 = less than 5%, 1 = between 5% and 15%,..., A = more than 95%)
38
BACK TO MAIN
NOTOX Project 242933
SYM-SUM - 3 1 7 - M A R - 99
CLINICAL SIGNS (SUMMARY)
MALES GROUP 2 (TELOMER ALCOHOL)
SIGN (MAX.GRADE)
TREATMENT WEEKS: 1 ............ 2 ............ 3 .............4 .............5. DAYS: 1.2.3.4.5.6.7.1.2.3.4.5.6.7.1.2.3.4.5.6.7.1.2.3.4.5.6 .7.1.
TIME: ABABABABABABABABABABABABABABABABABABABABABABABABABABABABAB
BREATHING
RALES (3)
SKIN / FOR / PLUMAGE
ALOPECIA (3) (CHEEK RIGHT)
ALOPECIA (3) (NECK)
ALOPECIA (3) (ABDOMEN)
ALOPECIA (3) (SHOULDER LEFT)
ALOPECIA (3) (FORELEG LEFT)
SCABS (3) (SNOUT)
SCABS (3) (BACK)
SCABS (3) (NECK)
WOUND (3) (GENITAL REGION)
RED STAINING (3) (PERIORBITAL REGION LEFT)
RED STAINING (3) (PERIORBITAL REGION RIGHT)
G: ....................................................... Ill %: ....................................................... Ill
G: ............................... 111111111...... %: ............................... 111111111......
G: .......................................... 11111 %: .......................................... 11111
G : ........... 1111111111111111111111111111111111111111111111
%: ........ 1111111111111111111111111111111111111111111111
...... .................................. 11 %: .... ................................. 11
G: 1111111 %: 1111111
G: ..... ....................................................1 %: .......... ...............................................1
G: 1 %: 1 .................................. ...... 1111
%: .......................................... 11......1111
G: 1 %: 1
G: %:
G: %:
G: Median value of the highest individual grades %: Percent of affected animals (0 = less than 5%, 1 = between 5% and 15%, . Time after treatment (H.Mi) A: 0.00 B: 4.00
A = more than 95%)
NOTOX Project 242933
CLINICAL SIGNS, DAILY (SUMMARY) MALES GROUP 2 (TELOMER ALCOHOL)
SIGN (MAX.GRADE) LOCATION
RECOVERY WEEKS: 1 ..... 2 ..... 3 ..... 4 ..... 5.
BREATHING RALES (3)
SK IN / FUR / PLUMAGE
ALOPECIA (3) (ABDOMEN) SCABS (3) (SNOUT) RED STAINING (3) (PERIORBITAL REGION LEFT) RED STAINING (3) (PERIORBITAL REGION RIGHT)
G: 1111111111111111..1111 % : 1222222222222223..3333
G: 11111 %: 12222 G: 11... %: 12... G: ...... Ill %: ...... 222 G: .... 1 ---
%: ... 2___
BACK TO MAIN
SYM-SUM - 4 25-MAR-99
G: Median value of the highest individual daily grades %: Percent of affected animals (0 = less than 5%, 1 = between 5% and 15%,.-.., A = more than 95%)
40
BACK TO MAIN
NOTOX Project 242933
SYM-SUM - o 17-MAR-99
CLINICAL SIGNS (SUMMARY)
MALES GROUP 3 (TELOMER ACRYLATE)
*
SIGN (MAX.GRADE)
TREAUffiNT
WEEKS: 1............ 2 .............3 ............ 4 .............5. DAYS: 1.2.3.4.5.6.7.1.2-3.4.5.6.7.1.2.3.4.5.6.7.1.2.3.4.5.6.7.1. TIME: ABABABABABABABABABABABABABABABABABABABABABABABABABABABABAB
SKIN / FOR / PLUMAGE
ALOPECIA (3) (NECK)
.
ALOPECIA (3) (FLANK LEFT)
ALOPECIA (3) (CHEST)
ALOPECIA (3) (FORELEG RIGHT)
SCABS (3) (BACK)
SCABS (3) (TAIL)
RED STAINING (3) (NECK)
G: %:
.......................;...................................m11i1l11l1..
G: 11112221111111111111................ %: 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 ................
G: 111222222211111111111111111111111111.
%: lllllllllllllllllllllllllllimillll.
G: 11122222221111111111................ %: 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 ................
G: ..................................... %: .....................................
G: ..................................... %: .....................................
G: .................................... %: .....................................
, . 1 1 1 1 .......... , . 1 1 1 1 ..........
..1 1 1 1 1 1 1 1 1 1 1 1 1 .. ,.1 1 1 1 1 1 1 1 1 1 1 1 1 ..
G: Median value of the highest individual grades %: Percent of affected animals (0 = less than 5%, 1 = between 5% and 15%,..., Time after treatment (H.Mi) A: 0.00 B: 4.00
41
A = more than 95%)
NOTOX Project 242933
CLINICAL SIGNS, DAILY (SUMMARY)
MALES GROUP 3 (TELOMER ACRYLATE)
SIGN (MAX.GRADE) LOCATION
RECOVERY WEEKS: 1 ..... 2 ..... 3 ..... 4 ..... 5.
SKIN / FOR / PLOMAGE
RED STAINING (3) (NECK)
G: ...................... 111111. %: ...................... 333333.
BACK TO MAIN
'S Y M - S O M - 6 25-MAR-9 9
G: Median, value of the highest individual daily grades %: Percent of affected animals (0 = less than 5%, 1 = between 5% and 15%,..., A = more than 95%)
BACK TO MAIN
NOTOX Project 242933
SYM-SUM - 7 1 7 - M A R - 99
CLINICAL SIGNS (SUMMARY) MALES GROUP 4
SIGN (MAX.GRADE)
TREATMENT
WEEKS: 1 ... ......... 2 ............ 3 ............ 4 .............5. DAYS: 1.2.3.4.5.6 .7.1.2.3.4.5.6 .7.1.2.3.4.5.6.7.1.2.3.4.5.6.7.1. TIME: ABABABABABABABABABABABABABABABABABABABABABABABABABABABABAB
SKIN / FOR / PLUMAGE
ALOPECIA (3) (EYE RIGHT)
ALOPECIA (3) (FORELEG RIGHT) SCABS (3) (NECK) RED STAINING (3) (PERIORBITAL REGION LEFT) VARIOUS EYE RIGHT, INJURED (1)
DEHYDRATED (3) (EYE RIGHT) BULGING EYE (1) (EYE RIGHT) DARK (3) (EYE RIGHT)
G: %: G: ..................................................... 11... %: ..................................................... 11... G: ................................ 111111111111 %: ................................ 111111111111 G: .................... 11111 %: .................... 11111
G:
%:
G: %: G: %: G: %:
G: Median value of the highest individual grades %: Percent of affected animals (0 = less than 5%, 1 = between 5% and 15%,..., A = more than 95%) Time after treatment (H.Mi) A: 0.00 B: 4.00
NOTOX Project 242933
CLINICAL SIGNS, DAILY (SUMMARY) MALES GROUP 4
SIGN (MAX.GRADE) LOCATION ,
RECOVERY WEEKS: 1 ..... 2 ..... 3 ..... 4 ..... S.
SKIN / FOR / PLUMAGE ALOPECIA (3) (EYE RIGHT) VARIOUS
EYE RIGHT, INJURED (1)
DEHYDRATED (3) (EYE RIGHT) BULGING EYE (1) (EYE RIGHT) DARK (3) (EYE RIGHT)
G: ........... 11U11U11111111. % ............. 2223333333333333.
G: 1111111111111111111111111111. %: 1222222222222223333333333333. G: .22222....................... %: .22222....................... G: 111111 %: 122222 G: 111111 %: 232222
BACK TO MAIN
SYM-SUM - S 2 5 - M A R - 99
G: Median value of the highest individual daily grades %: Percent of affected animals (0 = less than 5%, 1 = between 5% and 15%, ..., A = more than 95%)
BACK TO MAIN
NOTOX Project 242933
SYM-SUM - 5 1 7 - M AX-99
CLINICAL SIGNS (SUMMARY) MALES GROUP 5 (PFHS)
*
SIGN (MAX.GRADE)
TREATMENT
WEEKS: 1 ............ 2 .............3 ............ 4 .............5. DAYS: 1.2.3.4.5.6 .7.1.2.3.4.5.6 .7.1.2.3.4.5.S.7.1.2.3.4.5.S.7.1. TIME: ABABABABABABABABABABABABABABABABABABABABABABABAHABAEABAEAB
POSTURE
HUNCHED POSTURE (1)
SKIN / FUR / PLUMAGE
WOUND (3) (BACK)
RED STAINING (3) (EAR LEFT)
RED STAINING (3) (SNOUT)
RED STAINING (3) (NECK)
RED STAINING (3) (CERVICAL REGION)
VARIOUS
DARK (3) (EYE RIGHT)
G: ...................................................... 1111
%: ......... :................................. 1111
G: ............................ Ill %: ............................ Ill ........... 11111111111 %: ........ 11111111111 G: .................................................... 11 --%: .....................................................11 --G: %: G: %:
G: %:
G: Median value of the highest individual grades %: Percent of affected animals (0 = less than 5%, 1 = between 5% and 15%,.. Time after treatment (H.Mi) A: 0.00 B: 4.00
A = more than 95%)
NOTOX Project 242933
CLINICAL SIGNS, DAILY (SUMMARY) MALES GROUP 5 (PFHS)
SIGN (MAX.GRADE) LOCATION
RECOVERY WEEKS: 1 ..... 2 ..... 3 ..... 4 ..... 5.
POSTURE HUNCHED POSTURE (1)
SKIN / FUR / PLUMAGE RED STAINING (3) (NECK) RED STAINING (3) (CERVICAL REGION) VARIOUS DARK (3) (EYE RIGHT)
G: 11 %: 12
......... 11.................... %: ...... 22.................... G: ........ 1111111111111111111. %: .........2222223333333333333.
G: 11 %: 12
BACK TO MAIN
SYM-SUM -tO 25-MAR-9 9
G: Median value of the highest individual daily grades %: Petcent of affected animals (0 = less than 5%, 1 = between 5% and 15%,.... A = more than 95%)
BACK TO MAIN
NOTOX Proiect 242933
SYM-SUM -11 1 7 - M A R - 99
CLINICAL SIGNS (SUMMARY) MALES GROUP 6 (PFOS)
SIGN (MAX.GRADE)
TREATMENT
WEEKS: 1 ............ 2 ............ 3 ............ 4 ............ 5. DAYS: 1.2.3.4.5.6 .7.1.2.3.4.5.6.7.1.2.3.4.5.6 .7.1.2.3.4.5.6.7.1. TIME: ABABABABABABABABABABABABABABABABABABABABABABABABAEABABABAB
SKIN / FOR / PLUMAGE
SHELLING (3) (ABDOMEN)
ALOPECIA (3) (CHEEK LEFT)
SCABS (3) (NECK)
RED STAINING (3) (HEAD)
G: .................................................. 111111..
% ............. ...................................... min..
............. '........ 1 1 1 1 1 1 1 1 1 1 1 1 1 1 ..... '............
% ........................... 1 1 1 1 1 1 1 1 1 1 1 1 1 1 ..................
g : .................................m i n i ..................
%: .................................1 1 1 1 1 1 1 ..................
G: ..........................................................
.%: ................ .................................
G: Median value of the highest individual grades %: Percent of affected animals (0 = less than 5%, 1 = between 5% arid 1 5 % , . A = more than 95%) Time dfter treatment (H.Mi) A: 0.00 B: 4.00
NOTOX Project 242933
CLINICAL SIGNS, DAILY (SUMMARY) MALES . GROUP 6 (PFOS)
SIGN (MAX.GRADE) LOCATION
RECOVERY WEEKS: 1 ..... 2 ..... 3 ___ ...4......5.
SKIN / FUR / PLUMAGE
RED STAINING (3)
(HEAD)
......................111111111. %: .......... ........333333333.
BACK TO MAIN
S Y M - S U M -12. 2 5 - M A R - 99
G: Median value of the highest individual daily grades %: Percent of affected animals (0 = less than 5%, 1 = between 5% and 15%,...,
48
A = more than 95%)
BACK TO MAIN
NOTOX Project 242933
S Y M - S U M - 13 1 7 - M A R - 99
CLINICAL SIGNS (SUMMARY)
FEMALES
.
GROUP 1 (VEHICLE CONTROL)
.
SIGN (MAX.GRADE)
TREATMENT WEEKS: 1 ............ 2 ............ 3 ............ 4 .............5. DAYS: 1.2.3.4.5.5.7.1.2.3.4.5.6.7.1.2.3.4.5.6 .7.1.2.3.4.5.6.7.1. TIME: ABABABABABABABABABABABABABABABABABABABABABABABABABABABABAB
SKIN / FOR / PLUMAGE
ALOPECIA (3) (GENERAL)
ALOPECIA (3) (CHEEK LEFT)
ALOPECIA (3) (BACK)
ALOPECIA (3) (NECK)
ALOPECIA (3) (ABDOMEN)
ALOPECIA (3) (FLANK LEFT)
ALOPECIA (3) (FLANK RIGHT)
ALOPECIA (3) (SHOULDER LEFT)
ALOPECIA (3) (THIGH HIND LEFT)
ALOPECIA (3) (THIGH HIND RIGHT)
ALOPECIA (3) (HINDLEG LEFT)
SCABS (3) (ABDOMEN)
WOUND (3) (ABDOMEN)
SECRETION / EXCRETION
SALIVATION (3)
VARIOUS
MISSING (1) (TAIL APEX)
G: %:
G: %:
G: ...............................111111111 %: ................. -............ 111111111
G: %:
G: 1111222211111111111111111111111111111111111111111111111111 % : 1111111111111111111111111111111111111111111111111111111111
G: ....................................... 111111111111111111 %: ......................... ..............1111111111111111111
G: ........... 111111111111111.1111111111111111111111111111111 %: ........... 111111111111111.1111111111111111111111111111111
G: ............................. 1111111111111111111111 %: ....... ..................... 1111111111111111111111
G: ...11111111111........................... 111111111. %: ...11111111111........................... 111111111.
G: 11111111111111....................1111111111111111..... Ill %: 11111111111111.................... 1111111111111111.... Ill
G: ............... ..................... 111111111111111 %: .................................... 111111111111111
G: ................................................... 1111111 %: ............................................. ......1111111
..................................................... 1 ....... % .................................................... 1......
G: 1 %: 1
G: ......................... 1111111111111111111111111111111.. %: ......................... 1111111111111111111111111111111..
G: Median value of the highest individual grades %: Percent of affected animals (0 = less than 5%, 1 = between 5% and 15%,.... A = more than 95%) Time After treatment (H.Mi) A: 0.00 B: 4.00
NOTOX Project 242933
CLINICAL SIGNS, DAILY (SUMMARY)
FEMALES
.
GROUP 1 (VEHICLE CONTROL)
SIGN (MAX.GRADE) LOCATION
RECOVERY WEEKS: 1 ...... 2 ..... 3 ..... 4..... 5.
SKIN / FUR / PLUMAGE
ALOPECIA (3) (GENERAL)
ALOPECIA (3) (CHEEK LEFT)
ALOPECIA (3) (BACK)
ALOPECIA (3) (NECK)
ALOPECIA (3) (ABDOMEN)
ALOPECIA (3) (FLANK LEFT)
ALOPECIA (3) (FLANK RIGHT)
ALOPECIA (3) (THIGH HIND LEFT)
ALOPECIA (3) (THIGH HIND RIGHT) SCABS (3) (ABDOMEN)
G: ................ 11111111111 %: ................ 33333333333
G: ............ ..11.......... %: .............. 33..........
....... 11111111111........
%: ..... 22222222233..........
G: ......... 1111..............
%: ......... 2222...............
.........1 1 1 1 1 1 1 1 1 1 1 .......... %: ..... 22222222233....... .
G: 11111111111111111 %: 12222222222222233
G: 11122222222222222 %: 12222222333322233,
G: ...111111111111.., %: ...222222222222...
G: 111111111111111
%: 122222222222222
G: ...1111111111..
%: ...2222222222..
BACK TO MAIN
S Y M - S U M -l-g 25-MAR-99
G: Median value of the highest individual daily grades % : Percent of affected animals (0 = less than 5%, 1 = between 5% and 15%.... A = more than 95%)
50
BACK TO MAIN
T-l0\
NOTOX Project 242933
SYM-SUM 17-MAR-
CLINICAL SIGNS (SUMMARY)
FEMALES
.
GROUP 2 (TELOMER ALCOHOL)
*
SIGN (MAX.GRADE)
TREATMENT
WEEKS: 1 ............ 2 ............ 3 .............4 .............5. DAYS: 1.2.3.4.5.6 .7.1.2.3.4.5.6.7.1.2.3.4.5.6.7.1.2.3.4.5.6.7.1. TIME: ABABABABABABABABABABABABABABABABABABABABABABABABABABABABAB
SKIN / FUR / PLUMAGE
ALOPECIA (3) (HEAD)
ALOPECIA (3) (CHEEK LEFT)
ALOPECIA (3) (CHEEK RIGHT)
ALOPECIA (3) (BACK)
ALOPECIA (3) (NECK)
ALOPECIA (3) (FORELEGS)
SCALES (3) (CHEEK LEFT)
SCALES (3) (CHEEK RIGHT)
SCABS (3) (FLANK LEFT)
RED STAINING (3) (EAR RIGHT)
G: %:
G: ......... '............................. 1111111111........ %: ....................................... 1111111111........
G: 11 % ........................................................... 11
G: 11111 %: ...................................................... 11111
G: 11111111111111111....... 1 %: 11111111111111111....... 1
G: ...............................................1111111112.. %: ..................... ....................... ..1111111111..
G: %:
G: %:
G: .................................. 11 %: .................................. 11
G ............. 11 %: ........... 11
G: Median value of the highest individual grades % : Percent of affected animals (0 = less than 5%, 1 = between 5% and 15%,..., A = more than 95%) Time ^fter treatment (H.Mi) A: 0.00 B: 4.00
51
NOTOX Proiect 242933
CLINICAL SIGNS, DAILY. (SUMMARY)
FEMALES GROUP 2 (TELOMER ALCOHOL)
SIGN (MAX.GRADE) LOCATION
RECOVERY WEEKS: 1 ..... 2 ..... 3 ..... 4 ..... 5.
SKIN / FUR / PLUMAGE
ALOPECIA (3) (HEAD)
ALOPECIA (3) (CHEEK LEFT)
ALOPECIA (3) (CHEEK RIGHT)
SCALES (3) (CHEEK LEFT)
SCALES (3) (CHEEK RIGHT)
G: ...... 2222211 %:
G: .1112222222222 %: .2222333333333
G: ___ 1222222222 %: ___ 3333333333 G: ...... 1 1 1 1 1 1 1 %: ...... 2222222
G: ...... 1 1 1 1 1 1 1 %.:
BACK TO MAIN
SYM-SUM -*6 2 5 - M A R - 99
G: Median value of the highest individual daily grades %: Percent of affected animals (0 = less than 5%, 1 = between 5% and 15%,..., A = more than 95%)
BACK TO MAIN
NOTOX Proiect 242933
SYM-SUM - 17 1 7 - M A R - 99
CLINICAL SIGNS (SUMMARY)
FEMALES GROUP 3 (TELOMER ACRYLATE)
;
.
g-frai (MAX.GRADE)
TREATMENT
'
WEEKS: 1 ............ 2 ............ 3 ............ 4 .............5.
DAYS: 1.2.3.4.5.6 .7.1.2.3.4.5.6.7.1.2.3.4.5.6.7.1.2.3.4.5.6.7.1.
TIME: ABABABABABABABABABABABABABABABABABABABABABABABABABABABABAB
POSTURE HUNCHED POSTURE (1)
SKIN / FUR / PLUMAGE PILOERECTION (1)
ALOPECIA (3) (ABDOMEN)
ALOPECIA (3) (CHEST) ALOPECIA (3) (SHOULDER LEFT)
FISSURES WITH BLEEDINGS (3) (PAW HIND RIGHT) SCABS (3) (BACK) SCABS (3) (NECK)
RED STAINING (3) (HEAD)
RED STAINING (3) (BACK)
RED STAINING (3) (NECK) RED STAINING (3) (PAW HIND RIGHT)
G: .................. ...1111111111111................ 1111111
%: ......... :....... 1111111111111............ 1111111
G:
%:
G: 1111222211111111111111111111112122222211111111111111111111 %: 1111111111112222111111111133321111111122222232222222211111 G: ......................... 11111111111 %: ......................... 11111111111 G: ....................... 1111111...... %: ................ .......1111111..... G: .......................................11............... %: ....................................... 11................. G: 11
%: 11 G: 111111 %: 111111
G: 111............... 1111111 %: 111............... 1111111 G: 111111
%: 111111
G: 1111111111111........ 1111 %: 1111111111111......... 1111 G: 111111111111111 %: 111111111111111
G: Median value of the highest individual grades %: Percent of affected animals (0 = less than 5%, 1 = between 5% and 15%,..., Time After treatment (H.Mi) A: 0.00 B: 4.00
53
A = more than 95%)
N O T O X Pro-iect 242933
CLINICAL SIGNS, DAILY (SUMMARY)
FEMALES GROUP 3 (TELOMER ACRYLATE)
SIGN (MAX.GRADE) LOCATION
RECOVERY WEEKS: 1 ..... 2 ..... 3 ..... 4 ..... 5.
POSTURE HUNCHED POSTURE (1)
SKIN / FUR / PLUMAGE PILOERECTIQN (1)
ALOPECIA (3) (ABDOMEN) RED STAINING (3) (HEAD)
G: 11111111111111............. %: 23333332222222.............
G: .1111...................... %: .2222...................... G: 11111111111111............. %: 12222222222222............. G: ..........................11 % ............................77
BACK TO MAIN S Y M - S U M -IS
2 5 - M A R - 99
*
'
G: Median value of the highest individual daily grades %: Percent of affected animals (0 = less than 5%, 1 = between 5% and 15%,... A = more than 95%)
BACK TO MAIN
NOTOX Proiect 242933
SYM-SUM - 19 1 7 - M A R - 99
CLINICAL SIGNS (SUMMARY)
FEMALES GROUP 4
.
SIGN (MAX.GRADE)
TREATMENT
WEEKS: 1 ............ 2 ............ 3 ............ 4 ............ 5. DAYS: 1.2.3.4.5.6.7.1.2.3.4.5.6.7.1.2.3.4.5.6 .7.1.2.3.4.5.6 .7.1. TIME: ABABABABABABABABABABABA8ABABABABABABABABABABABAHABAEABABA3
POSTURE HUNCHED POSTURE (1)
G:
%:
1111111111111111111111111111 1111111111111111111111111111
SKIN / FUR / PLUMAGE
SWELLING (3) (EAR RIGHT)
...............1111111111111 %: ........... 1111111111111
ALOPECIA (3) (BACK)
ALOPECIA (3) (NECK)
G: ................. 111111111111... 1111111111111.... 1111111.. % : ................. 111111111111... 1111111111111___ 1111111..
G: 111111111111111111111111111111111111 %: 111111111111111111111111111122222222
ALOPECIA (3) (THROAT REGION)
G: %:
222222222222222222222222222222221111 111111111111111111111111111111111111
SCALES (3) (THROAT REGION)
G: ....................... 1111111111111 %: ....................... 1111111111111
SCABS (3) (NECK)
G: %:
RED STAINING (3) (EVE RIGHT) '
G: %:
RED STAINING (3) (PERIORBITAL REGION RIGHT)
G: %:
RED STAINING (3) (EAR LEFT)
G: ...................... 111111111 %: ...................... 111111111
RED STAINING (3) (EAR RIGHT)
G: ........... 1111111111111111111111111111111111111111111111
% : ............1111111111111111111111111111111111112211111111
SECRETION / EXCRETION
WATERY DISCHARGE FROM EYE (3) G:
(EYE RIGHT) .
%:
G: Median value of the highest individual grades
.-
%: Percent of affected animals (0 = less than 5%, 1 = between 5% and 15%,..., A = more than 95%)
Time after treatment (H.Mi) A: 0.00 B: 4.00
55
NOTOX Proiect 242933
CLINICAL SIGNS, DAILY (SUMMARY) FEMALES GROUP 4
SIGN (MAX.GRADE) LOCATION
RECOVERY WEEKS: 1 ..... 2 ..... 3 ..... 4 ..... 5.
SKIN / FOR / PLOMAGE
SCABS (3) (NECK)
G ........................... . %: ......................... 3.
RED STAINING (3) (EYE RIGHT)
G: ...11...... I............... %: ...22......................
RED STAINING (3) (PERIORBITAL REGION RIGHT)
G: .....11......... 1111....... %: .....22......... 3333.......
SECRETION / EXCRETION
.
WATERY DISCHARGE FROM EYE (3) G: ........ .....11............
(EYE RIGHT)
%: ............. 23...........
BACK TO MAIN
S Y M - S U M - 2,0 2 5 - M A R - 99
G: Median value of the highest individual daily grades %: Percent of affected animals (0 = less than 5%, 1 = between 5% and 15%,..., A = more than 95%)
56
BACK TO MAIN
NOTOX Proiect 242933
S Y M - S U M - 2.1 1 7 - M A R - 99
CLINICAL SIGNS (SUMMARY) FEMALES GROUP 5 (PFHS)
`
SIGN (MAX.GRADE)
treatment
WEEKS: 1 ............ 2 ............ 3 ............ 4 .............5. DAYS: 1.2.3.4.5.6 .7.1.2.3.4.5.6 .7.1.2.3.4.5.6 .7.1.2.3.4.5.6.7.1. TIME: ASABABABABABABABABABABABABABABABABABABABABABABABABABABABAB
POSTURE
HUNCHED POSTURE (1)
SKIN / FUR / PLUMAGE
ALOPECIA (3) (BACK)
ALOPECIA (3) (NECK)
ALOPECIA (3) (ABDOMEN)
ALOPECIA (3) (SHOULDER RIGHT)
ALOPECIA (3) (HINDLEG RIGHT)
SCABS (3) (NECK) WOUND (3) (NECK)
SECRETION / EXCRETION
SALIVATION (3)
G:
%:
G: ..................... 11111112111111.
%: ................. 11111111111111.
G: ..111111111222222111111111111111111111111112222222222222.. %.: ..111111111111111111113333333344333322222222211111111111..
G: .1111111111222222222222222222222222111111111111 %: .1111111111111111111111111111111111111111111111
G:
%:
G:
%:
G: 1111112222111111111111222222111111111111111111111111111. % : 1111111111111111111111111111111111111111111111111111111. G: ....... 11.. 111112222211.
%: ..... 11..111111111111.
G:
%:
G: Median value of the highest individual grades %: Percent of affected animals (0 = less than 5%, 1 = between 5% and 15%,..., A = more than 95%) Time after treatment (H.Mi) A: 0.00 B: 4.00
57
NOTOX Prolect 242933
.
CLINICAL SIGNS, DAILY (SUMMARY) FEMALES GROUP 5 (PFHS)
SIGH (MAX.GRADE) LOCATION
RECOVERY WEEKS: 1 ..... 2 ..... 3 ..... 4 ..... 5.
*
SKIN / FUR / PLUMAGE ALOPECIA (3) (BACK) ALOPECIA (3) (NECK) ALOPECIA (3) (ABDOMEN) ALOPECIA (3) (HINDLEG RIGHT)
G: ......................... 11 %: ......................... 33
G: 111..1111111U1111111111111 % : 122..22222333337777777777777
G: 111--- 111111111111111111111 %: 122--- .222222223333333333333
G: ..... 1111.................. %: ..... 2222..................
-
BACK TO MAIN
SYM-SUM - 22 25-MAR-99 :
.
G: Median value of the highest individual daily grades %: Percent of affected animals {0 = less than 5%, 1 = between 5% and 15%,....
58
A = more than 95%)
BACK TO MAIN
NOTOX Project 242933
SYM-SUM - 13 17 -MA?.- 99
CLINICAL SIGNS (SUMMARY)
FEMALES GROUP 6 (PFOS)
.
SIG N (MAX.GRADE)
TREATMENT
hjEreyq. 2............ 2 ..... ...... 3........... 4............ 5.
days:*
TIME: ABABABABABABABABABABABABABABABABABABABABABABABABABABABABAB
POSTORE HUNCHED POSTURE )
SKIN / FOR / PLUMAGE ALOPECIA (3) (ABDOMEN) ALOPECIA (3) (FORELEG LEFT) ALOPECIA (3) (FORELEG RIGHT) SCABS (3) (NECK) RED STAINING (3) (HEAD) RED STAINING (3) (EAR LEFT) RED STAINING (3) (EAR RIGHT) RED STAINING (3) (SNOOT) RED STAINING (3) (NECK)
G: %:
G: %: G: %:
G: %:
G: %: G: %: G: %: G: %: G: %: G: %:
11111111 11111111 1111111111111111111111111111...111111111111 1111111111111111111111111111...111111111111 ......................... 111111112. . 222222222222222.. ........... 111111111..111111111111111.. ........... 222222222..222222222222222.. ........... 111111111..111111111111111.. ................. 1111111........... ................. 1111111...........
1111.. . 1111.. . lili lili
G: Median value of the highest individual grades %: Percent of affected animals (0 = less than 5%, 1 = between 5% and 15%,..., A = more than 95%) Time after treatment (H.Mi) A: 0.00 B: 4.00
NOTOX Proiect 242933
CLINICAL SIGNS, DAILY (SUMMARY) FEMALES GROUP 6 (PFOS)
S IG N (MAX.GRADE)
LOCATION
RECOVERY
WEEKS'. 1 ..... 2 ..... 3 ..... 4 ..... 5.
SKIN / FUR / PLUMAGE ALOPECIA (3) (ABDOMEN) RED STAINING (3) (HEAD) RED STAINING (3) (EAR LEFT) RED STAINING (3) (EAR RIGHT) RED STAINING (3) (NECK)
G: 11111........
%: 12222.......
G: 11111111111111 %: 12255332222233
G: ...111111111 %: ...222222222
G: 111.. %: .222..
........... 11111 %: 22222
BACK TO MAIN
SYM-SUM - 14 2 5 - M A R - 99
} !
G: Median value of the highest individual daily grades %: Percent of affected animals {0 = less than 5%, 1 = between 5% and 1 5 % , A = more than 95%)
60
NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/
-PFHS/PFOS
'
BACK TO MAIN
BW-SUM - 1 09-DEC-98
BODY WEIGHTS (GRAM) SUMMARY MALES
TREATMENT
DAY 1 WEEK 1
MEAN ST.DEV.
N
DAY 5 WEEK 1
DAY 8 WEEK 2
DAY 12 WEEK 2
DAY 15 WEEK 3
DAY 19 WEEK 3
DAY 22 WEEK 4
DAY 25 WEEK 4 .
DAY 28 WEEK 4
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
GROUP 1 VEHICLE CONTROL
208 14.0 14
232 14.9 14
248 19.8 14
272 20.7 14
281 25.2 14
302 26.8 14
312 27.4 14
324 29.8 14
336 29.9 14
GROUP 2 TELOMER ALCOHOL
212 13.5 14
237 14.4 14
252 17.6 14
277 18.6 14
288 22.9 14
308 23.2 14
316 23.6 14
329 25.4 14
340 27.5 14
GROUP 3 TELOMER ACRYLATE
GROUP 4
209 210 9.2 9.4
14 14
234 8.9 14
248 11.8 14
273 9.3 14
283 10.9 14
304 10.3 14
315 9.5 14
328 12.6 14
338 13.0 14
238 10.8 14
252 13.2 14
279 17.7 14
288 19.4 14
312 24.1 14
319 26.5 14
333 28.4 14
347 31.6 14
GROUP 5 PFHS
208 13.8 14
231 16.5 14
246 23.4 14
270 24.5 14
275 26.3 14
301 30.4 14
307 31.9 14
321 34.8 14
328 37.1 14
GROUP 6 PFOS
210 10.9 14
237 13.9 14
252 17.8 14
279 21.0 14
288 25.2 14
310 29.0 14
318 31.4 14
333 33.5 14
342 33.7 14
* / ** : Dunnett-Test based on pooled variance significant at 5% (*) or 1% (**) level
61
NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/
-_ PFHS/PFOS
BACK TO MAIN
BW-SUM - 2 . 09-DEC-98
BODY WEIGHTS (GRAM) SUMMARY MALES
RECOVERY
DAY 1 WEEK 1
DAY 5 WEEK 1
DAY 6 WEEK 2
DAY 12 WEEK 2
DAY 14 WEEK 2
DAY 19 WEEK 3
DAY 22 WEEK 4
DAY 25 WEEK 4
DAY 28 WEEK 4
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
GROUP 1 VEHICLE
CONTROL
GROUP 2 TELOMER ALCOHOL
304 27.9 6
338 31.2 6
346 32.8 6
358 32.8 6
362 32.4 6
391 24.6 3
399 24.2 3
412 25.2 3
415 26.7 3
298 20.2 6
331 28.8 6
338 28.1 6
350 28.5 6
351 28.6 6
375 . 35.7
3
382 33.5 3
391 38.5 3
391 37.1 3
GROUP 3 TELOMER ACRYLATE
GROUP 4
GROUP 5 PFHS
314 12.4 6
351 23.5 6
356 20.9 6
372 21.0 6
374 20.8 6
389 7.2 3
399 5.5 3
407 7.8 3
412 2.6 3
330 28.2 6
372 31.8 6
383 35.2 6
400 36.9 6
398 38.0 6
419 35.0 3
426 37.6 3
437 38.7 3
435 37.6 3
291 40.4 6
327 45.4 6
338 46.9 6
344 48.5 -6
345 47.6 6
347 19.1 3
351 19.1 3
368 20.8 3
362 20.8 3
GROUP 6 PFOS
313 40.4 6
352 46.8 6
360 46.0 6
374 46.8 6
376 47.6 6
351 39.7 3
359 38.6 3
371 34.8 3
370 39.3 3
* / ** : Dunnett-Test based on pooled variance significant at 5% (*) or 1% (**) level
NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/.
-PFHS/PFOS
'
BACK TO MAIN
BW-SUM - 3 09-DEC-98
BODY WEIGHTS (GRAM) SUMMARY FEMALES
TREATMENT
DAY 1 WEEK 1
DAY 5 WEEK 1
DAY 8 WEEK 2
DAY 12 WEEK 2
DAY 15 WEEK 3
DAY 19 WEEK 3
DAY 22 WEEK 4
DAY 25 WEEK 4
DAY 28 WEEK 4
MEAN ST.DEV. N
MEAN ST. DEV. N
MEAN ST.DEV. N
MEAN ST. DEV. N
MEAN ST. DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
GROUP 1 VEHICLE
CONTROL
GROUP 2 TELOMER ALCOHOL
174
11.9 14
181 . 14.3 14
188 15.6 14
202 16.2 14
.
206 19.8 14
219 20.1 14
221 19.4 14
232 21.4 14
236 21.8 14
169 8.0 14
176 8.5 14
182 11.9 14
194 11.9 14
200 15.0 14
213 15.5 14
216 15.7 14
227 16.5 14
230 16.9 14
GROUP 3 TELOMER ACRYLATE
GROUP 4
166 10.0 14
173 11.2 14
179 13.2 14
191 13.3 14
192 15.0 14
203 15.5 14
207 16.9 14
218 17.3 14
219 * 17.0 14
170 11.9 14
177 14.5 14
182 15.9 14
198 16.8 14
202 18.2 14
214 19.6 14
218 20.4 14
230 18.6 14
231 20.5 14
GROUP 5 PFHS
169 8.0 14
174 10.4 14
180 9.8 14
194 10.9 14
196 11.3 14
209 11.3 14
212 12.6 14
222 13.0 14
224 12.6 14
GROUP 6 PFOS
166 8.7 14
171 9.8 14
176 10.1 14
188 * 9.8 14
187 * 11.5 14
198 ** 12.7 14
200 ** 13.2 14
207 ** 14.3 14
207 * 13.3 14
* / ** : Dunnett-Test based on pooled variance significant at 5% (*) or 1% (**) level
63
.
NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/
.,, PFHS/PFOS
BACK TO MAIN
BW-SUM - 4 09-DEC-98
BODY WEIGHTS (GRAM) SUMMARY FEMALES
RECOVERY
GROUP 1 VEHICLE
CONTROL
GROUP 2 TELOMER ALCOHOL
GROUP 3 TELOMER ACRYLATE
GROUP 4
GROUP 5 PFHS
GROUP 6 PFOS
DAY 1 WEEK 1
DAY 5 WEEK 1
DAY 8 WEEK 2
DAY 12 WEEK 2
DAY 14 WEEK 2
DAY 19 WEEK 3
DAY 22 WEEK 4
DAY 25 WEEK 4
DAY 28 WEEK 4
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
213 21.9 6
239 24.1 6
244 25.1 6
251 25.8 6
257 27.3 6
254 27.5 3
258 28.0 3
263 28.6 3
268 27.8 3
200 9.9 6
226 11.3 6
229 12.7 6
238 12.3 6
237 16.7 6
246 13.2 3
251 12.9 3
251 10.8 3
251 12.7 3
193 16.2 6
219 18.2 6
222 19.9 6
233 20.2 6
231 20.6 6
245 26.6 3
248 25.3 3
249 25.4 3
248 26.0 3
212 23.9 6
236 20.3 6
240 21.1 6
248 23.0 6
248 20.0 6
253 29.1 3
258 30.3 3
265 31.0 3
262 35.5 3
206 12.8 6
231 14.4 6
235 14.1 6
243 12.8 6
241 13.6 6
243 12.9 3
246 14.5 3
254 17.3 3
254 13.2 3
185 * 7.0 6
207 * 9.5 6
213 * ' 8.6
6
219 * ' 8.2
6
220 ** 5.5 6
226 11.3 3
224 9.8 3
226 10.7
3
229 13.0 3
* / ** : Dunnett-Test based on pooled variance significant at 5% (*) or lit (**) level
64
NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/
-PFHS/PFOS
'
BACK TO MAIN
BWG-SUM - 1 09-DEC-98
BODY WEIGHT GAIN (%) SUMMARY MALES
TREATMENT
DAY 1 WEEK 1
DAY 5 WEEK 1
DAY 8 WEEK 2
DAY 12 WEEK 2
DAY 15 WEEK 3
DAY 19 WEEK 3
DAY 22 WEEK 4
DAY 25 WEEK 4
DAY 28 WEEK 4
MEAN ST. DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST. DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
GROUP 1 VEHICLE CONTROL
0 0.0 14
12 6.3 14
19 7.4 14
31 7.9 14
35 9.5 14 .
45 10.1 14
50 10.4 14
56 11.2 14
61 11.4 14
GROUP 2 TELOMER ALCOHOL
0 0.0 14
12 1.9 14
19 3.3 14
30 4.8 14
36 6.5 14
45 7.6 14
49 7.9 14
55 8.8 14
60 9.1 14
GROUP 3
TELOMER ACRYLATE
GROUP 4
00 0.0 0.0 14 14
12 13 1.5 2.3 14 14
19 20 3.7 3.5 14 14
31 33 4.7 5.5 14 14
35 37 5.0 6.5 14 14
45 48 5.8 9.0 14 14
51 52 5.7 9.9 14 14
57 58 6.5 11.4 14 14
62 65 7.1 12.9 14 14
GROUP 5 PFHS
0 0.0 14
11 2.2 14
18 4.6 14
30 4.5 14
32 7.2 14
45 6.7 14
48 7.3 14
54 8.5 14
58 9.6 14
GROUP 6 PFOS
0 0.0 14
13 1.5 14
20 4.2 14
33 4.8 14
37 7.4 14
47 8.9 14
51 10.3 14
58 10.8 14
62 11.1 14
* / * : Dunnett-Test based on pooled variance significant at 5% (*) or 1% (**) level
65
NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/
-PFHS/PFOS
"
BACK TO MAIN
BWG-SUM - 2 09-DEC-98
BODY WEIGHT GAIN (%) SUMMARY MALES
RECOVERY
DAY 1 WEEK 1
DAY 5 WEEK 1
DAY 8 WEEK 2
DAY 12 WEEK 2
DAY 14 WEEK 2
DAY 19 WEEK 3
DAY 22 WEEK 4
DAY 25 WEEK 4
DAY 28 WEEK 4
MEAN ST. DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
GROUP 1 VEHICLE CONTROL
51 7.9 6
68 9.7 6
72 9.8 6
78 9.1 6
80 9.0 6
92 6.4 3
97 5.7 3
103 6.2 3
105 6.5 3
GROUP 2 TELOMER ALCOHOL
45 5.8 6
61 8.7 6
65 8.6 6
71 8.2 6
71 8.9 6
77 13.0 3
80 12.0 3
84 14.2 3
84 * 13.5 3
GROUP 3 TELOMER ACRYLATE
GROUP 4
51 57 3.8 10.6 66
69 78 7.4 12.3 66
72 83 6.8 12.8 66
79 91 7.3 14.1 66
80 90 7.0 15.8 66
88 93 7.1 5.8 33
93 97 6.3 6.8 33
97 102 6.9 7.3 33
99 101 6.1 7.6 33
GROUP 5 PFHS
45 12.6 6
63 13.9 6
69 14.4 6
72 15.6 6
72 15.2 6
77 7.1 3
79 * 7.2 3
87 9.9 3
84 * 8.1 3
GROUP 6 PFOS
49 14.5 6
68 16.5 6
71 16.5 6
78 16.6 6
79 17.2 6
70 * 5.6 3
74 * 4.9 3
80 ' 2.7 3
80 * 4.5 3
/ ** : Dunnett-Test based on pooled variance significant at 5H (*) or 1H (**) level
66
NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/
-PFHS/PFOS
'
BACK TO MAIN
BWG-SUM - 3 09-DEC-98
BODY WEIGHT GAIN (%) SUMMARY FEMALES
TREATMENT
GROUP 1 VEHICLE CONTROL
GROUP 2 TELOMER ALCOHOL
GROUP 3 TELOMER
ACRYLATE
GROUP 4
GROUP 5 PFHS
GROUP 6 PFOS
OAY 1
MEAN
0
0
00
0
0
WEEK 1
ST.DEV.
0.0
0.0
0.0
0.0
0.0
0.0
N 14 14 14 14 14 14 .
DAY 5
MEAN
4
5
4
4
3
3
WEEK 1
ST.DEV.
2.7
2.5
2.3
2.3
3.2
3.4
N 14 14 14 14 14 14
DAY 8
MEAN
8
8
87
7
6
WEEK 2
ST.DEV.
3.2 ' 3.9
2.6
3.2
2.9
2.7
N
14
14
14
14
14
. 14
DAY 12
MEAN
16
15
15 17 14
13
WEEK 2
ST.DEV.
4.3
4.2
2.9
3.5
3.4
4.0
N . 14 14 14 14 14 14
DAY 15
MEAN
18 18 16 19 16 13
WEEK 3
ST.DEV.
5.5
5.8
4.3
4.2
3.7
4.6
N 14 14 14 14 14 14
DAY 19
MEAN
26
26 23
26
23
19
WEEK 3
ST. DEV.
5.6
5.8
5.3
4.2
3.9
6.4
N 14 14 14 14 14 14
DAY 22
MEAN
27 28
25 28 26
21 *
WEEK 4
ST.DEV.
4.5
5.5
6.2
4.6
4.5
6.9
N 14 14 14 14 14 14
DAY 25
MEAN
33
34 31 35 31
24 **
WEEK 4
ST.DEV.
6.7
5.8
5.6
3.9
4.3
7.3
N 14 14 14 14 14 14
DAY 28
MEAN
36 36 32 36 33
25 **
WEEK 4
ST.DEV.
6.7
7.0
4.8
4.5
4.9
7.5
N 14 14 14 14 14 14
/ * * . : Dunnett-Test based on pooled variance significant at 5% (*) or 1% (**) level
67
NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/
.
-PFHS/PFOS
'
BACK TO MAIN
BWG-SUM - 4 09-DEC-98
BODY WEIGHT GAIN (%) SUMMARY FEMALES
RECOVERY
GROUP 1 VEHICLE CONTROL
GROUP 2 TELOMER ALCOHOL
GROUP 3 TELOMER ACRYLATE
GROUP 4
GROUP 5 PFHS
GROUP 6 PFOS
DAY 1
MEAN
23
21
18 25 22
15
WEEK 1
ST.DEV.
5.7
3.7
7.7 4.4
5.0
6.1
N 6 6 .6 6 6 6
DAY 5
MEAN
37
37
35 39 37
29
WEEK 1
ST.DEV.
6.4
4.6
9.5
3.7
6.1
7.7 .
N 6 6 666 6
DAY 8
MEAN
41
39
36 41 39
33
WEEK 2
ST.DEV.
7.0
5.4
9.9 '5.7
6.2
7.3
N 6 6 666 6
DAY 12
MEAN
44
44
43 46
44
37
WEEK 2
ST.DEV.
7.2
4.8
9.8
6.1
7.2
7.2
N 6 6 '6 6 6 6
DAY 14
MEAN
48
43
42 46
43
37
WEEK 2
ST.DEV.
8.8
7.8
9.8 4.6
8.5
5.9
N 6 6 666 6
DAY 19
MEAN
47
50
48 45
45
41
WEEK 3
ST.DEV.
12.5
7.0
4.8 5.1
8.6
10.3
N 3 3 333 3
DAY 22
MEAN
50
53
50 48 47
39
WEEK 4
ST.DEV.
12.1
6.7
6.2
3.7
9.5
9.5
N 3 3 333 3
DAY 25
MEAN
53
53
50 52
52
40
WEEK 4
ST.DEV.
12.7
5.4
3.8 2.8
7.6
10.0
N 3 3 333 3
DAY 28
MEAN
56
53
50 50 52
42
WEEK 4
ST.DEV.
13.8
6.2
3.0 5.6
8.8
11.5
N 3 3 333 3
/ ** : Dunnett-Test based on pooled variance significant at 5% (*) or 1* (**) level
68
NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/
-_ PFHS/PFOS
BACK TO MAIN
FC-SUM - 1 0 1 -MAR-99
FOOD CONSUMPTION (G/ANIMAL/DAY) SUMMARY
MALES
TREATMENT
DAYS 1-8 WEEKS 1/2
DAYS 8-15 WEEKS 2/3
DAYS 15-22 WEEKS 3/4
DAYS 22-28 WEEK 4
MEAN ST.DEV. N (CAGE)
MEAN ST.DEV. N (CAGE)
MEAN ST.DEV. N (CAGE)
MEAN ST.DEV. N (CAGE)
GROUP 1 VEHICLE CONTROL
26 2.1 4
28 2.7 4
30 ' 3.3 4
30 2.3 4
GROUP 2 TELOMER ALCOHOL
' GROUP 3 TELOMER ACRYLATE
GROUP 4
26 27 26
1.9 1.0 1.0 4 44
28 28 29 2.4 0.9 0.6 4 44
29 30 30 1.3 1.5 2.3 444
30
31 .
31
1.5 1.1 2.4
4 44
GROUP 5 PFHS
26 2.4 4
27 2.1 4
29 1.2 4
30 1.6 4
GROUP 6 PFOS
27 0.9 4
29 1.6 4
30 2.7 4
31 3.0 4
MEAN OF MEANS OVER TREATMENT
MEAN
GROUP 1 VEHICLE CONTROL
28
GROUP 2 TELOMER ALCOHOL
28
GROUP 3 TELOMER ACRYLATE
GROUP 4
29 29
GROUP 5 PFHS
28
GROUP 6 PFOS
29
/ ** : Dunnett-Test based on pooled variance significant at 5% (*) or 1% (**) level
NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/
-PFHS/PFOS
'
BACK TO MAIN
FC-SUM - 2 01-MAR-99
FOOD CONSUMPTION (G/ANIMAL/DAY) SUMMARY MALES
RECOVERY
DAYS 1-8 WEEKS 1/2
DAYS 8-14 WEEK 2
DAYS 14-19 WEEKS 2/3
DAYS 19-22 WEEKS 3/4
DAYS 22-28 WEEK 4
MEAN ST.DEV. N (CAGE)
MEAN ST.DEV. N (CAGE)
MEAN ST.DEV. N (CAGE)
MEAN ST.DEV. N (CAGE)
MEAN ST.DEV. N (CAGE)
GROUP 1 VEHICLE CONTROL
30 2.5 2
30 1.8 2
30
---
1
31
---
1
31
--
1
GROUP 2 TELOMER ALCOHOL
28 2.0 2
28 1.8 2
28
..
1
29
---
1
28
---
1
GROUP 3 TELOMER ACRYLATE
GROUP 4
30 32 1.8 1.4 22
31 31
1.4 2.0 22
30 30
---
- .-
11
32 31
...
11
30
- -
29
--- -
11
GROUP 5 PFHS
30 0.8 2
29 1.2 2
26
...
1
27
...
1
27
...
1
GROUP 6
pros
30 3.7 2
30 4.0 2
27
...
1
28
...
1
27
--- .
1
MEAN OF MEANS OVER RECOVERY
MEAN
GROUP 1 VEHICLE CONTROL
30
GROUP 2 TELOMER ALCOHOL
28
GROUP 3 TELOMER
ACRYLATE
GROUP 4
30 30
GROUP 5 PFHS
28
GROUP 6 PFOS
28
* / ** : Dunnett-Test based on pooled variance significant at 5% (*) or 1% (*') level 70
NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/
-PFHS/PFOS
.
BACK TO MAIN
FC-SUM - 3 01-MAR-99
FOOD CONSUMPTION (G/ANIMAL/DAY) SUMMARY FEMALES
TREATMENT
DAYS 1-8 WEEKS 1/2
DAYS 8-15 WEEKS 2/3
DAYS 15-22 WEEKS 3/4
DAYS 22-28 WEEK 4
MEAN ST.DEV. N (CAGE)
MEAN ST.DEV. N (CAGE)
MEAN ST.DEV. N (CAGE)
MEAN ST.DEV. N (CAGE)
GROUP 1 VEHICLE CONTROL
19 0.7 4
21 0.5 4
22 1.3 4
24 1.2 4
GROUP 2 TELOMER ALCOHOL
18 1.2 4
20 0.5 4
22 0.8 4
23 0.7 4
GROUP 3 TELOMER ACRYLATE
GROUP 4
19 19 1.2 0.6 44
20 21 1.1 0.8 44
22 22 1.2 1.1 44
23 23 1.3 0.5 44
GROUP 5 PFHS
20 1.6 4
21 1.4 4
22 1.3 4
23 0.7 4
GROUP 6 PFOS
19 1.9 4
19 0.8 4
20 * 1.0 4
20 ** 0.5 4
MEAN OF MEANS OVER TREATMENT
MEAN
GROUP 1 VEHICLE CONTROL
22
GROUP 2 TELOMER ALCOHOL
21
GROUP 3 TELOMER ACRYLATE
GROUP 4
21 21
GROUP 5 PFHS
22
GROUP 6 PFOS
19
* / ** : Dunnett-Test based on pooled variance significant at 5% () or 1% (**) level
71
NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/
-_ PFHS/PFOS
BACK TO MAIN
FC-SUM - 4 0 1 -MAR-99
F O O D C O N S U M P T I O N (G/ANIMAL/DAY) S U M M A R Y FEMALES
RECOVERY
DAYS 1-8 WEEKS 1/2
DAYS 8-14 WEEK 2
DAYS 14-19 WEEKS 2/3
DAYS 19-22 WEEKS 3/4
DAYS 22-28 WEEK 4
MEAN ST.DEV. N (CAGE)
MEAN ST.DEV. N (CAGE)
MEAN ST.DEV. N (CAGE)
MEAN ST.DEV. N (CAGE)
MEAN ST.DEV. N (CAGE)
GROUP 1 VEHICLE CONTROL
25 0.4 2
24 0.2 2
22 ..1
24 ... 1
24 ... 1
GROUP 2 TELOMER ALCOHOL
24 1.0 2
23 1.1 2
22 ... 1
23 ..1
22 -- 1
GROUP 3 TELOMER ACRYLATE
GROUP 4
24 24 1.2 1.1 22
23 23 0.8 1.2 22
22 20 ... ... 11
22 21 ... ... 11
22 22 ... ... 11
GROUP 5 PFHS
24 0.6 2
24 0.6 2
20
1
22
1
21
1
GROUP 6 PFOS
21 ** 0.2 2
20 * 0.3 2
19
1
19
1
19
1
MEAN OF MEANS OVER RECOVERY
MEAN
GROUP 1 VEHICLE CONTROL
24
GROUP 2 TELOMER ALCOHOL
23
GROUP 3 TELOMER ACRYLATE
GROUP 4
22 22
GROUP 5 PFHS
22
GROUP 6 PFOS
20
/ ** : Dunnett-Test based on pooled variance significant at 5% (*) or 1% (**) level
NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/
PFHS/PFOS'
BACK TO MAIN
Kl-U-UM - 1 0 1 -MAR-99
RELATIVE FOOD CONSUMPTION SUMMARY (G/KG BODY WEIGHT/DAY) MALES
TREATMENT
DAYS 1-8 WEEKS 1/2
DAYS 8-15 WEEKS 2/3
DAYS 15-22 WEEKS 3/4
DAYS 22-28 WEEK 4
MEAN ST.DEV. N (CAGE)
MEAN ST.DEV. N (CAGE)
MEAN ST.DEV. N (CAGE)
MEAN ST.DEV. N (CAGE)
GROUP 1 VEHICLE CONTROL
114 8.7 4
103 9.3 4
99 11.1 4
93 6.8 4
GROUP 2 TELOMER ALCOHOL
GROUP 3 TELOMER ACRYLATE
GROUP 4
112 3.6 4.
102 3.3 4
96 4.5 4
91 1.1 4
115 3.8 4
103 2.0 4
99 5.2 4
94 3.4 4
111 2.9 4
102 2.3 4
96 5.9 4
92 5.3 4
GROUP 5 PFHS
112 7.1 4
101 2.6 4
98 5.5 4
93 3.6 4
GROUP 6 PFOS
115 3.7 4
104 1.9 4
98 6.8 4
94 6.1 4
MEAN OF MEANS OVER TREATMENT
MEAN
GROUP 1 VEHICLE CONTROL
102
GROUP 2 TELOMER ALCOHOL
100
GROUP 3 TELOMER ACRYLATE
GROUP 4
103 100
GROUP 5
GROUP 6
PFHS ' PFOS
101 103
/ ** : Dunnett-Test based on pooled variance significant at 5% (*) or 1% (**) level
NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/
-. PFHS/PFOS
BACK TO MAIN
RFC-SUM - 2 01-MAR-99
RELATIVE FOOD CONSUMPTION SUMMARY (G/KG BODY WEIGHT/DAY) MALES
RECOVERY
DAYS 1-8 WEEKS 1/2
DAYS 8-14 WEEK 2
DAYS 14-19 WEEKS 2/3
DAYS 19-22 WEEKS 3/4
DAYS 22-28 WEEK 4
MEAN ST.DEV. N (CAGE)
MEAN ST.DEV. N (CAGE)
MEAN ST.DEV. N (CAGE)
MEAN ST.DEV. N .(CAGE)
MEAN ST.DEV. N (CAGE)
GROUP 1 VEHICLE CONTROL
90 1.5 2
84 0.1 2
77 --1
78 ... 1
75 -- 1
GROUP 2 TELOMER ALCOHOL
GROUP 3 TELOMER ACRYLATE
GROUP 4
85 87 0.9 4.9
2 2
80 82 0.7 2.6 22
74 76 . . . 11
76 80 -- . 11
72 74 -- ... 11
85 4.1 2
78 5.8 2
71 ... 1
72 ... 1
67 ... 1
GROUP 5 PFHS
91 0.3 2
83 0.1 2
76 ... 1
77 ... 1
73 ... 1
GROUP 6
pros
86 2.1 2
81 0.2 2
76 ... 1
78 ... 1
72 ...
MEAN OF MEANS OVER RECOVERY
' MEAN
GROUP 1 VEHICLE CONTROL
81
GROUP 2 TELOMER ALCOHOL
77
GROUP 3 TELOMER
ACRYLATE
GROUP 4
80 74
GROUP 5 PFHS
80
GROUP 6 PFOS
79
* / ** : Dunnett-Test based on pooled variance significant at 5% (*) or 1% (**) level 74
NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/
-PFHS/PFOS
''
BACK TO MAIN
RFC-SUM 3 01-MAR-99
RELATIVE FOOD CONSUMPTION SUMMARY (G/KG BODY WEIGHT/DAY) FEMALES
TREATMENT
GROUP 1 VEHICLE CONTROL
GROUP 2 TELOMER ALCOHOL
GROUP 3 TELOMER ACRYLATE
GROUP 4
DAYS 1-8 WEEKS 1/2
DAYS 8-15 WEEKS 2/3
DAYS 15-22 WEEKS 3/4
DAYS 22-28 WEEK 4
MEAN ST.DEV. N (CAGE)
MEAN ST.DEV. N (CAGE)
MEAN ST.DEV. N (CAGE)
MEAN ST.DEV. N (CAGE)
106 1.2 4
104 2.4 4
103 6.8 4
102 6.1 4
104 6.1 4
105 2.8 4
105 6.7 4
102 4.6 4
110 5.0 4
107 4.7 4
108 5.7 4
105 2.8 4
108 5.4 4
107 4.7 4
105 6.2 4
101 3.4 4
GROUP 5 PFHS
113 11.8 4
109 8.0 4
107 4.9 4
104 1.9 4
GROUP 6 PFOS
111 11.4 4
103 6.4 4
100 7.9 4
95 4.6 4
MEAN OF MEANS OVER TREATMENT
MEAN
GROUP 1 VEHICLE CONTROL
104
GROUP 2 TELOMER ALCOHOL
104
GROUP 3 TELOMER ACRYLATE
GROUP 4
107 105
GROUP 5 PFHS
108
GROUP 6 PFOS
102
/ ** : Dunnett-Test based on pooled variance significant at 5% {*) or 1% (**) level
NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/
-PFHS/PFOS
"
BACK TO MAIN
RFC-SUM - 4 01-MAR-99
RELATIVE FOOD CONSUMPTION SUMMARY (G/KG BODY WEIGHT/DAY) FEMALES
RECOVERY
DAYS 1-8 WEEKS 1/2
DAYS 8-14 WEEK 2
DAYS 14-19 WEEKS 2/3
DAYS 19-22 WEEKS 3/4
DAYS 22-28 WEEK 4
MEAN ST.DEV. N (CAGE)
MEAN ST.DEV. N (CAGE)
MEAN ST.DEV. N (CAGE)
MEAN ST.DEV. N (CAGE)
MEAN ST.DEV. N (CAGE)
GROUP 1 VEHICLE CONTROL
105 3.5 2
95 0.2 2
86 -- 1
92 1
91
1
GROUP 2 TELOMER ALCOHOL
104 2.7 2
95 3.7 2
88 -- 1
92 -- 1
86 --1
GROUP 3 TELOMER
ACRYLATE
GROUP 4
107 102 2.4 5.3 22
97 94 1.0 4.0 22
88 81 ---- 11
87 83 ---- 11
90 81 ---- 11
GROUP 5 PFHS
106 1.2 2
97 0.2 2
84 -- 1
88
--
1
84 -- 1
GROUP 6 PFOS
100 4.0 2
93 3.9 2
85 --1
86 -- 1
85 .-1
MEAN OF MEANS OVER RECOVERY
MEAN
GROUP 1 VEHICLE CONTROL
94
GROUP 2 TELOMER ALCOHOL
93
GROUP 3 TELOMER ACRYLATE
GROUP 4
94 88
GROUP 5 PFHS
92
GROUP 6 PFOS
90
* / * : Dunnett-Test based on pooled variance significant at 5% (*) or 1% (**) level
----------- -
NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/
.
-PFHS/PFOS
"
BACK TO MAIN
HEM-SUM - 1 0 9 -NOV-98
HAEMATOLOGY SUMMARY AFTER 4 WEEKS MALES
TREATMENT
HAEMATOLOGY PARAMETERS
RBC T/1
HB mmol/I
HCT 1/1
MCV fl
MCH fmol
MCHC mmol/I
ROW %
PLATELETS G/l
WBC G/l
SEG.
1
EO. 1
BASO.
1
MONO. 1
LYMPH. 1
MEAN ST. DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN S T '.DEV. N
MEAN ST.DEV. N
GROUP 1 VEHICLE CONTROL
GROUP 2 TELOMER ALCOHOL
GROUP 3 TELOMER ACRYLATE
GROUP 4
GROUP 5 PFHS
GROUP 6 PFOS
7.05 0.24 10
9.1 0.4 10
0.407
0.011
10
57.8 1.1 10
1.294 0.036 10
22.4 0.4 10
12.4 0.4 10
1182 110 10
11.3 3.5 10
0.124 0.033 10
0.007 0.005 10
0.000 0.000 10
0.026 0.006 10
0.844 0.030 10
6.72 0.32 14
8.8 0.4 14
0.395 0.016 14
58.8 1.4 14
1.313 0.030 14
22.3 0.4 14
12.0 0.7 14
1025 116 14
10.0 2.0
14
0.125 0.041 14
0.011 0.011
14
0.000 0.000 14
0.022 0.012 14
0.841 0.048 14
6.61 * 0.27 14
6.79 0.32 13
8.7 8.8
0.4 0.5 14 13
0.388 0.014 14
0.397 0.021 13
58.7
1.0
14
58.5 1.2 13
1.317 0.027 14
1.297 0.045 13
22.4
0.5 14
22.2 0.4 13
12.2 0.6 14
12.0 0.3 13
1115 158 14
1050 158 13
10.2 2.1
14
11.7 2.4 13
0.117
0.035 14
0.087 +
0.025 13
0.007
0.005 14
0.007 0.004 - 13
0.000
0.000
14
0.000 0.000
13
0.020 0.010 14
0.023
0.009 13
0.857
0.040 14
0.883 + 0.031
13
6.57 '* 0.52 14
8.5 ** 0.6 14
0.383 * 0.026 14
58.4 1.8 14
1.297 0.035 14
22.2 0.4 14
12.0 0.7 14
870 ** 306 14
9.7 3.0 14
0.148 0.049 14
0.008 0.007 14
0.000
0.000 14
0.026 0.013 14
0.818 0.053 14
6.66 0.39 12
8.9 0.4 12
0.392 0.019 12
59.0 1.9 12
1.338 0.047 12
22.7 0.4 12
12.0 0.8 12
1092 75 12
10.7 2.2 12
0.114 0.039 12
0.005 0.005 12
0.000 0.000
12
0.023 0.009 12
0.858 0.041 12
*/**: Dunnett -test based on pooled variance s1g. at 5% or 1% level . + : Steel test slg. at 5% level.
77
NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/'
-PFHS/PFOS
" ^
'
BACK TO MAIN
HEM-SUM 2 0 9 -NOV-98
HAEMATOLOGY SUMMARY AFTER 4 WEEKS MALES
TREATMENT
GROUP 1
VEHICLE CONTROL
HAEMATOLOGY PARAMETERS
PT MEAN SEC ST.DEV.
N
PTT MEAN SEC ST.DEV.
N
14.9 0.6 13
16.1 0.8 13
GROUP 2 TELOMER ALCOHOL
15.0 0.6 14
16.0 0.7 14
GROUP 3 TELOMER ACRYLATE
GROUP 4
14.9 0.6 13
17.1 2.2 13
14.4 0.6 12
18.2 3.2 11
GROUP 5 PFHS
14.1 ** 0.4 9 16.8 0.8 9
GROUP 6 PFOS
14.7 0.6 13 17.2 1.8 13
*/**: Dunnett-test based on pooled variance s1g. at 5% or 1% level. +: Steel-test s1g. at 5% level.
78
NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/
'PFHS/PFOS
BACK TO MAIN
HEM-SUM - 3 09-N0V-98
HAEMATOLOGY SUMMARY AFTER 4 WEEKS FEMALES
TREATMENT
GROUP 1
VEHICLE CONTROL
GROUP 2 TELOMER ALCOHOL
GROUP 3 TELOMER ACRYLATE
GROUP 4
GROUP 5 PFHS
GROUP 6 PFOS
HAEMATOLOGY PARAMETERS
RBC MEAN
6.72
6.27 '*
6.61
6.62
6.35 *
6.41
T/l
ST.OEV.
0.14
0.39
0.34
0.36
0.28
0.37
N 11 13 13 13 13 11
HB
MEAN
9.1
8.5 *
8.8
9.0
8.7
8.6
m m o 1/1
ST.OEV.
0.3
0.5
0.5 0.4
0.4
0.5
N 11 13 13 13 13 11
HCT MEAN
0.378
0.355 **
0.370
0.377
0.358 *
0.359*
1/1
ST.OEV.
0.012
0.018
0.019
0.014
0.017
0.019
N 11 13 13 13 13 11
MCV n
MCH fmol
MEAN ST.OEV. N
MEAN ST.OEV. N
56.2 1.9 11
1.350 0.034 11
56.6 1.4 13
1.356 0.035 13
56.0 1.7 13
1.329 0.049 13
57.1 1.9 13
1.363 0.053 13
56.3 2.6 13
1.356 0.050 13
56.0 1.9 11
1.348 0.041 11
MCHC mmol/I
MEAN ST.OEV. N
24.0 0.5 11
24.0 0.6 13
23.7 0.6 13
23.9 0.5 13
24.3 0.5
13
24.1 0.2 11
ROW MEAN
11.3
11.2
11.4
11.3
11.3
11.2
%
ST.DEV.
0.4
0.5
0.6
0.3
0.6
0.5
N
11
13
13
13
13
11
PLATELETS G/l
MEAN ST.DEV. N
1166 127 11
1141
149 13
1149 125 13
1169 74
13
1035 236 13
1134 131 11
WBC `
MEAN
7.4 7.5 6.8 7.6 6.3 8.4
G/l
ST.DEV.
2.1
1.6
2.3
1.8
1.9
2.4
N 11 13 13 13 13 11
SEG. 1
EO. 1
MEAN ST.DEV. N
MEAN ST.DEV. N
0.082 0.027 11
0.010 0.007 11
0.103 0.041 13
0.004 0.005 13
0.109 0.039
13
0.009 0.009 13
0.087 0.024 13
0.011 0.006 13
0.080 ' 0.035
13
0.009 0.006 13
0.077 0.020 11
0.005 0.004 11
BASO. 1
MEAN ST.DEV. N
0.000 0.000
11
0.000 0.000 13
0.000 0.000 13
0.000 0.000 13
0.000 0.000 13
0.000 0.000 11
MONO. 1
MEAN ST.DEV. N
0.025 0.011
11
0.020 0.011 13
0.017
0.009 13
0.024 0.008 13
0.024 0.012
13
0.020 0.007
11
LYMPH.
MEAN
0.883
0.873
0.865
0.877
0.887
0.898
1
ST.DEV.
0.034
0.044
0.040
0.030
0.039
0.026
N 11 13 13 13 13 11
*/*': Dunnett test based on pooled variance s1g. at 5% or 1% level . +: Steel test s1g. at 5% level.
79
NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/
'" PFHS/PFOS
BACK TO MAIN
HEM-SUM - 4 09-NOV-98
HAEMATOLOGY SUMMARY AFTER 4 WEEKS FEMALES
TREATMENT
HAEMATOLOGY PARAMETERS
PT MEAN SEC ST.DEV.
N PTT MEAN SEC ST.DEV.
N
GROUP 1 VEHICLE CONTROL
GROUP 2 TELOMER ALCOHOL
GROUP 3 TELOMER ACRYLATE
GROUP 4
GROUP 5 PFHS
15.4 1.0 14
15.9 1.4 14
15.0 0.6 14
15.0 0.9 14
15.0 0.8 13
15.7 0.8 12
14.5 % 0.5 13
17.0 0.9 13
14.5 0.7 12
16.8 2.0 11
GROUP 6 PFOS
14.4 0.4 14 16.5 2.2 14
*/**: Dunnett-test based on pooled variance s1g. at 5% or 1% level. +: Steel-test s1g. at 5% level.
80
NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/
-PFHS/PFOS
'
BACK TO MAIN
HEM-SUM - 5 0 9 -D E C -98
HAEMATOLOGY SUMMARY AFTER 2 WEEKS RECOVERY MALES
TREATMENT HAEMATOLOGY PARAMETERS
GROUP 1 VEHICLE CONTROL
GROUP 2 TELOMER
ALCOHOL
GROUP 3 TELOMER ACRYLATE
GROUP 4
GROUP 5 PFHS
GROUP 6 PFOS
RBC MEAN
7.23
7.04
6.97
7.00
6.97
7.01
T/l
ST.DEV.
0.18
0.25
0.27
0.27
0.21
0.22
N 3 3 333 3
HB mmol/1
HCT 1/1
MEAN ST.DEV. N
MEAN ST.DEV. N
9.1 0.4 3
0.417 0.011 3
8.9 0.1 3
0.404 0.008 3
8.7 0.5 3
0.401 0.018 3
8.8 0.1 3
0.402 0.014 3
8.6 0.1 3
0.392 0.004 3
8.9 0.0 3
0.408 0.006 3
MCV MEAN
57.7
57.3
57.5
57.4
56.3
58.2
fl
ST.DEV.
0.1
1.0
0.6 0.3
1.6
1.0
N 33 333 3
MCH fmol
MEAN ST.DEV. N
1.259 0.036 3
1.260 0.037 3
1.253 0.028 3
1.259 0.034 3
1.230
0.020 3
1.270 0.041
3
MCHC mmol/1
MEAN
ST.DEV. N
21.8 0.6
3
22.0 0.3 3
21.8 0.3 3
21.9 0.5 3
21.8 0.3 3
21.8 0.3 3
RDW MEAN
14.1
13.7
13.4
13.3
14.4
13.1
%
ST.DEV.
0.5
0.6
0.8
1.3
0.5
0.8
N 33 333 3
PLATELETS G/l
MEAN ST.DEV. N
1177 106 3
1081 102 3
1186 170 3
1080
49 3
1053 22 3
1078 58 3
WBC MEAN
8.5
12.0
12.1
12.2
8.5
11.6
G/l
ST.DEV.
1.8
3.2
1.9
1.8
2.4
1.9
N 3 3 333 3
SEG. 1
MEAN ST.DEV.
N
0.112 0.043 3
0.127 0.073 3
0.088 0.048 3
0.058
0.019 3
0,133 0.063 3
0.137 0.016 3.
EO. MEAN
0.013
0.005
0.005
0.003
0.012
0.002
1
ST.DEV.
0.003
0.005
0.005
0.003
0.003
0.003
N 33 333 3
BASO. 1
MEAN
ST.DEV. N
0.000
0.000 3
0.000
0.000 3
0.000 0.000 3
0.000
0.000 3
0.000 0.000 3
0.000 0.000 3
MONO. 1
MEAN ST.DEV. N
0.027 0.003 3
0.022 0.008 3
0.025 0.005 3
0.023
0.006 3
0.018 0.008 3
0.018 0.003 3
LYMPH.
MEAN
0.848
0.847
0.882
0.915
0.837
0.843
1
ST.DEV.
0.043
0.077
0.055
0.022
0.073
0.020
N 33 333 3
/**: Dunnett -test based on pooled variance s1g. at 5% or 1% level!. +: Steel -test sig. at 5% level
81
NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/
*PFHS/PFOS
'
BACK TO MAIN
HEM-SUM - 6 0 9 -DEC-98
HAEMATOLOGY SUMMARY AFTER 2 WEEKS RECOVERY MALES
TREATMENT
HAEMATOLOGY PARAMETERS
PT MEAN SEC ST.DEV.
N PTT MEAN SEC ST.DEV.
N
GROUP 1 VEHICLE CONTROL
GROUP 2 TELOMER
ALCOHOL
14.6 0.4 3
16.2 1.1 3
15.2 0.5 3
15.3 0.7 3
GROUP 3 TELOMER ACRYLATE
GROUP' 4
GROUP 5 PFHS
14.9 0.7 3
16.0 1.0 3
14.6 0.6 3
15.0 1.3 3
15.2 , 0.4
2
15.2 0.2 2
GROUP 6 PFOS
15.5 0.5 3 14.7 1.6 3
/**: Dunnett-test based on pooled variance $1g. at 5% or 1% level. +: Steel-test s1g. at 5% level.
NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/
'PFHS/PFOS
BACK TO MAIN
HEM-SUM - 7 0 9 -DEC-98
HAEMATOLOGY SUMMARY AFTER 2 WEEKS RECOVERY FEMALES
TREATMENT
GROUP 1 VEHICLE CONTROL
GROUP 2 TELOMER ALCOHOL
GROUP 3 TELOMER ACRYLATE
GROUP 4
GROUP 5 PFHS
GROUP PFOS
HAEMATOLOGY PARAMETERS
RBC MEAN
7.02
6.47
6.62
6.71
6.61
' 6.53
T/l
ST.DEV.
0.09
0.19
0.23
0.51
0.35
0.33
N 3 3 3. 3 3 3
HB MEAN 9.0 8.4 8.7 9.0 8.6 8.5
mmol/I
ST.DEV.
0.2
0.2
0.3
0.4
0.5
0.4
N 333333
HCT MEAN
0.404
0.378
0.389
0.396
0.383
0.387
1/1
ST.DEV.
0.009
0.005
0.012
0.012
0.030
0.016
N 333333
MCV MEAN
57.5
58.4
58.9
59.2
57.9
59.3
fl
ST.DEV.
0.7
1.7
2.8
2.7
2.1
0.6
N 333333
MCH fmol
MEAN ST. DEV. N
1.277 0.021 3
1.305 0.060 3
1.310
0.055 3
1.339 0.048 3
1.301 0.041 3
1.307 0.012 3
MCHC mmol/I
MEAN ST.DEV. N
22.2 0.2 3
22.4 0.4 3
22.3 0.2 3
22.6 0.4
3
22.5 0.5 3
22.1 0.2 3
ROW MEAN
14.5
15.2
15.4
15.3
14.6
15.7
%
ST.DEV.
1.5
1.1
1.3
1.7
1.1
0.1
N 33333 3
PLATELETS G/l
MEAN ST.DEV. N
1232 165
3
1117 144 3
1066 133 3
1111 56 3
854 * 145 3
1034 233 3
WBC MEAN
7.3 8.7 7.3 6.7 6.6 6.8
G/l
ST.DEV.
1.8
2.8
3.3
1.3
1.0
2 .5
N 33 3333
SEG. 1
MEAN
ST.DEV. N
0.075 0.035 3
0.077 0.020 3
0.065 0.018 3
0.080 0.033 3
0.050 0.030
3
0.100 0.015
3
EO. 'MEAN
0.005
0.007
0.003
0.010
0.008
0.002
1
ST.DEV.
0.005
0.008
0.003
0.005
0.010
0.003
N 333333
BASO. 1
MEAN ST.DEV. N
0.000 0.000 3
0.000 0.000 3
0.000 0.000 3
0.000 0.000. 3
0.000 0.000 3
0.000 0.000 3
MONO. 1
MEAN ST.DEV. N
0.017 0.008 3
0.025 0.010 3
0.017 0.013
3
0.033 0.013 3
0.010 0.009
3
0.027 0.008
3
LYMPH.
MEAN
0.903
0.892
0.915
0.877
0.932
0.872
1
ST.DEV.
0.038
0.023
0.022
0.023
0.032
0.010
N 33 3333
*/*: Dunnett -test based on pooled variance s1g. at 5% or 1% level . +: Steel -test s1g: at 5% levi
83
NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/
*PFHS/PFOS
'
BACK TO MAIN
HEM-SUM - 8
09-DFr.cjfl
HAEMATOLOGY SUMMARY AFTER 2 WEEKS RECOVERY FEMALES
TREATMENT HAEMATOLOGY PARAMETERS
GROUP 1
VEHICLE CONTROL
GROUP 2
TELOMER ALCOHOL
GROUP 3
TELOMER ACRYLATE
GROUP 4 .
GROUP 5 PFHS
GROUP 6 PFOS
PT SEC
MEAN ST.DEV. N
16.0 0.4 3
14.9 0.4 3
15.6
0.6 3
14.8 * 0.3 3
14.8 * 0.1 2
15.5 0.1 2
PTT SEC
MEAN ST.DEV. N
'
15.5 2.0 3
14.4 0.3 3
15.1
1.1 3
14.8 0.9 3
15.7 1.3 2
13.7 0.0 2
*/*: Dunnett-tast based on pooled variance s1g. at 5% or 1% level. +: Steel-test s1g. at 5% level.
84
NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/
'PFHS/PFOS
"
BACK TO MAIN
HEM-SUM - 9 09-DEC-98
HAEMATOLOGY SUMMARY AFTER 4 WEEKS RECOVERY MALES
TREATMENT
GROUP 1 VEHICLE CONTROL
GROUP 2 TELOMER ALCOHOL
GROUP 3 TELOMER
ACRYLATE
GROUP 4
GROUP 5 PFHS
GROUP 6 PFOS
HAEMATOLOGY PARAMETERS
RBC MEAN
7.35
7.01
6.85
7.01
6.89
6.99
T/1
ST.DEV.
0.50
0.14
0.16
0.17
0.08
0.31
N 3 3 333 3
HB MEAN 9.1 9.1 9.1 9.2 9.0 9.0
mmol/1
ST.DEV.
0.5
0.2
0.3
0.3
0.4
0.2
N 33 333 3
HOT MEAN
0.396
0.391
0.384
0.392
0.390
0.389
1/1
ST.DEV.
0.026
0.005 .
0.012
0.015
0.014
0.015
N 3 3 333 3
MCV MEAN
53.9
55.8
56.1
55.8
56.7
55.7
fl
ST.DEV.
1.6
0.8
1.6 0.8
1.4
1.8
N 33 333 3
MCH fmol
MEAN ST.DEV.
N
1.243 0.052
3
1.294 0.037 3
1.323 0.020 3
1.317 0.031 3
1.307 0.042 3
1.284 0.041
3
MCHC mmol/1
MEAN ST.DEV. N
23.1
0.3 3
23.2 0.3 3
23.6 0.4 3
23.6 0.6 3
23.1 0.2 3
23.1 0.3 3
ROW MEAN
14.5
12.9 *
13.7
12^9 *
13.6
13.7
%
ST.DEV.
0.4
0.5
0.3
0.4 0.3
0.9
N 33 333 3
PLATELETS G/l
MEAN ST.DEV. N
1082 113 3.
1025 95 3
1076 149 3
1179
70 3
966
71 3
1077 27 3
WBC MEAN
12.2
12.3
10.8
9.4
7.7
11.2
G/1
ST.DEV.
0.9
3.0
4.2
1.3
1.6
3.3
N 33 333 3
SEG. 1
MEAN ST.DEV. N
0.103
0.053 3
0.095 0.028 3
0.090 0.033 3
0.060 0.009 3
0.063 0.003
3
0.078 0.046 3
EO. MEAN
0.007
0.003
0.010
0.005
0.007
0.007
1
ST.DEV.
0.006
0.006
0.005
0.005
0.003
0.003
N 3 3 333 3
BASO. 1
MEAN ST.DEV.
N
0.000 0.000 3
0.000
0.000 3
0.000 0.000 3
0.000 0.000 3
0.000 0.000 3
0.000 0.000 3
MONO. 1
MEAN ST.DEV.
N
0.052 0.025 3
0.055 0.018 3
0.047 0.033 3
0.020 0.009 3
0.025 0.009 3
0.027 0.020
3
LYMPH.
MEAN
0.838
0.847
0.853
0.915
0.905
0.888
1
ST.DEV.
0.056
0.050
0.068
0.005
0.013
0.059
N 33 333 3
*/**: Dunnett -test based on pooled variance s1g. at 5% or 1% level . +: Steel test s1g. at 5% level
85
NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/
'/PFHS/PFOS
"
BACK TO MAIN
HEM-SUM -10 09-DEC-98
HAEMATOLOGY SUMMARY AFTER 4 WEEKS RECOVERY MALES
TREATMENT
HAEMATOLOGY PARAMETERS
PT MEAN SEC ST.DEV.
N PTT MEAN SEC ST.DEV.
N
GROUP 1 VEHICLE CONTROL
GROUP 2 TELOMER ALCOHOL
GROUP 3 TELOMER ACRYLATE
GROUP 4
GROUP 5 PFHS
GROUP 6 PFOS
13.1 0.6 2
16.0 0.1 2
13.0 0.4 2
15.2 0.8 2
13.4 0.2 3
16.8 0.2 3
13.8 1.1 2
16.5 0.8 2
14.2 0.5 2
15.2 0.4 2
14.0 0.2 3
14.6 2.0 3
*/**: Dunnett-test based on pooled variance slg. at 5% or 1% level. +: Steel-test sig. at 5% level.
86
NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/
' -' /PFHS/PFOS
"
BACK TO MAIN
HEM-SUM -11 '09-DEC-98
HAEMATOLOGY SUMMARY AFTER 4 WEEKS RECOVERY FEMALES
TREATMENT
GROUP 1 VEHICLE CONTROL
GROUP 2
GROUP 3
TELOMER . TELOMER
ALCOHOL
ACRYLATE
GROUP 4
GROUP 5 PFHS
GROUP 6 PFOS
HAEMATOLOGY PARAMETERS
RBC MEAN
6.87
6.86
6.79
6.99
6.80
6.56
T/l
ST.DEV.
0.19
0.09
0.27
0.22
0.25
0.15
N 333333
HB MEAN 9.3 9.4 9.0 9.1 8.9 8.7
mmol/1
ST.DEV.
0.1
0.2 ' 0.2
0.2
0.3
0.6
N 3 3 3 .3 3 3
HOT MEAN
0.397
0.402
0.389
0.393
0.389
0.379
1/1
ST.DEV.
0.023
0.002
0.009
0.008
0.022
0.030
N 3 33333
MCV MEAN
57.8
58.7
57.4
56.2
57.2
57.8
fl
ST.DEV.
1.8
1.1
2.3
0.9
3.4
3.3
N 333333
MCH fmol
MEAN ST.DEV. N
1.349 0.020 3
1.366 0.018 3
1.322 0.033 3
1.297
0.030 3
1.314 0.049 3
1.321 0.058 3
MCHC mmol/I
MEAN ST.DEV. N
23.4
1.0
3
23.3 0.4 3
23.1 0.4 3
23.1
0.2 3
23.0 0.5 3
22.9 0.6 3
RDW MEAN
12.8
13.5
13.3
12.6
13.3
12.9
%
ST.DEV.
0.8
0.5
0.2
0.3
1.1
0.9
N 333333
PLATELETS MEAN
972
980
965
1014
978
994
G/l
ST.DEV.
54
103 126 40
127 80
N 333333
WBC MEAN
6.0 8.8 7.7 6.2 6.8 8.7
G/l
ST.DEV.
0.6
1.1 3.7 0.4 2.3 2.3
N 333333
SEG. 1
MEAN ST.DEV. N
0.053 0.031 3
0.070
0.013 3
0.038 0.015 3
0.077 0.045 3
0.075 0.017 3
0.062 0.025
3
EO. 1
BASO. 1
MEAN ST.DEV. N
MEAN ST.DEV. N
0.008 0.010 3
0.000
0.000
3
0.003 0.003 3
0.000
0.000 3
0.010 0.000 3
0.000
0.000 3
0.020 0.000 3
0.000
0.000 3
0.007 0.003 3
0.000 0.000
3
0.005 0.009 3
0.000
0.000 3
MONO. 1
MEAN ST.DEV.
N
0.015 0.005 3
0.027 0.008 3
0.018 0.014 3
0.030 0.000 3
0.035 0.018
3
0.017 0.008 3
LYMPH. 1.
MEAN ST.DEV.
N
0.923 0.032
3
0.900 0.005
3
0.933 0.010 3
0.873 0.045 3
0.883 0.030 3
0.917 0.033 3
*/**: Dunnett -test based on pooled variance s1g. at 5% or 1% level . +: Steel -test s1g. at 5% level.
NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/
'/PFHS/PFOS
BACK TO MAIN
HEM-SUM -12 09-DEC-98
HAEMATOLOGY SUMMARY AFTER 4 WEEKS RECOVERY FEMALES
TREATMENT
GROUP 1 VEHICLE CONTROL
GROUP 2 TELOMER ALCOHOL
GROUP 3 TELOMER ACRYLATE
GROUP 4
GROUP 5
GROUP 6
PFHS . PFOS
HAEMATOLOGY PARAMETERS
PT MEAN SEC ST.DEV.
N
PTT MEAN SEC ST.DEV.
N
12.3 0.6 3
15.7 1.2 3
12.2 0.3 3
14.6 0.5 3
11.9 0.4 3
15.7 1.1 3
12.2 0.6 3
15.0 0.6 3
11.7 0.3 3
15.3 0.2 3
12.2 0.4 3
14.9 1.1 3
*/**: Dunnett-test based on pooled variance s1g. at 5% or 1% level. +: Steel-test s1g. at 5% level.
88
NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/
.
-PFHS/PFOS
'
"" BACK TO MAIN
CHE-SUM - 1 09-N0V-98
CLINICAL BIOCHEMISTRY SUMMARY
AFTER 4 WEEKS MALES
TREATMENT
ALAT(GPT) ukat/1
ASAT(GOT) ukat/1
Bill T. umol/1
CHOLEST.T. mraol/1
TRIGL. mmol/1
CREATININE umol/1
GLUCOSE mmol/1
UREA mmol/1
PROTEIN T. o/i
ALBUMIN g/i
GLOBULIN 0/1
A/G RATIO
ALP ukat/1
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
GROUP 1 VEHICLE CONTROL
0.64 0.12 14
2.80 0.38 14
2 1 14
2.00 0.39 14
0.80 0.25 14
33. 4 14
6.32 0.75 14
6.9 1.1 14
56.8 2.9
14 .
29.3 0.9 14
27.5 2.5 14
1.1 0.1 14
7.76 1.75 14
GROUP 2 TELOMER ALCOHOL
0.61 0.10 14
2.62 0.41 14
2 0 14
1.28 ** 0.44 14
0.63 0.29 14
30 3 14
6.48 0.95 14
7.0 1.0 14
53.2 ** 2.1 14
28.7 1.3 14
24.4 + 1.8 14
1.2 0.1 14
8.08 1.94 14
GROUP 3 TELOMER
ACRYLATE
GROUP 4
0.61
0.14 13
2.58 0.46 14
2 1 14
1.43 **
0.47 14
0.73
0.39 14
31
2 14
6.24 0.73 14
6.7
1.1 14
53.9 *
2.9 14
29.3 1.7 14
24.6 + 2.0 14
1.2 +
0.1 14
7.70 1.14 14
0.57 0.11 13
2.31 * 0.30 14
2 0 14
1.76 0.40 14
0.65 0.21 14
34 4 14
6.59 1.07 14
6.9 1.0 14
53.8 * 2.2 14
29.8 1.3 14
24.0 + 1.5 14
1.2 + 0.1 14
8.00 1.16 14
GROUP 5 PFHS
0.67 0.25 14
2.39 ' 0.26 14
2 0 14
0.97 ** 0.20 14
0.43 ** 0.15 14
35 5 14
6.72 1.47 14
7.7 1.5 14
53.7 ** 2.8 14
30.4 1.4 14
23.3 + 2.4 14
1.3 + 0.1 14
9.33 * 1.50 14
GROUP 6 PFOS
0.59 0.06 14
2.37 * 0.45 14
2 1 14
1.13 * 0.40 14
0.48 ** 0.17 14
32 3 14
6.38 1.24 14
7.6 1.2 14
54.1 * 2.0 14
29.3 1.2 14
24.8 + 2.1 14
1.2 + 0.1 14
7.26 1.48 14
*/**: Dunnett -test based on pooled variance s1g. at 5% or 1% level. +: Steel-test slg. at 5% level.
NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/
'PFHS/PFOS
"
BACK TO MAIN
CHE-SUM - 2 O9-N0V-98
CLINICAL BIOCHEMISTRY SUMMARY AFTER 4 WEEKS MALES
TREATMENT
GROUP 1 VEHICLE CONTROL
. GROUP 2 TELOMER ALCOHOL
INORG PHOSPH MEAN
mmol/1
ST.DEV.
N
SODIUM mmol/1
MEAN ST.DEV. N
POTASSIUM mmol/1
MEAN ST.DEV. N
CALCIUM mmol/1
MEAN ST.DEV.
N
CHLORIDE mmol/1
MEAN ST.DEV. N
2.83 0.16 14
142.6 1.3 14
4.85 0.31 14
2.53 0.08 14
100 2 14
2.75 0.13 14 '
142.9 0.9 14
4.60 0.27 14
2.47 0.06 14
100 2 14
GROUP 3 TELOMER ACRYLATE
GROUP 4
2.88 0.19 14
143.2 0.7 14
4.72 0.39 14
2.50 0.06 14
102 1 14
2.71 0.23 14
143.0 0.7 14
4.50 * 0.22 14
2.47 0.07 14
101 2 14
GROUP 5 PFHS
3.02 0.30 14
143.3 0.8 14
4.55 0.46 14
2.51 0.08 14
103 ** 2 14
GROUP PFOS
2.96 0.20 14
142.9 0.9 14
4.66 0.35 14
2.52 0.08 14
103 *' 3 14
*/**: Dunnett-test based on pooled variance s1g. at 5% or 1% level. +: Steel-test s1g. at 5% level.
90
NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/
'PFHS/PFOS
"
BACK TO MAIN
CHE-SUM 3 09-N0V-98
CLINICAL BIOCHEMISTRY SUMMARY AFTER 4 WEEKS FEMALES
TREATMENT
ALAT(GPT) ukat/1
A S A T (G O T ) ukat/1
BILI T. umol/1
CHOLEST.T. mmol/1
TRIGL. mmol/1
CREATININE umol/1
GLUCOSE mmol/1
UREA mmol/1
PROTEIN T.
gn
ALBUMIN g/1
GLOBULIN g/1
A/G RATIO
ALP ukat/1
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N.
MEAN ST.DEV. N
MEAN ST.OEV. N
MEAN ST.DEV. N
MEAN ST.OEV. N
MEAN ST.DEV. N
MEAN ST.OEV. N
GROUP 1 VEHICLE CONTROL
0.52 0.12 14
2.62 0.34 14
3 1 14
2.21 0.44 14
0.60 0.18 14
40 3 14
6.05 0.52 14
8.3 1.8 .14
65.9 3.3 14
34.0 1.4 14
31.9 2.2 14
1.1 0.1 14
4.46 0.62 14
GROUP 2 TELOMER ALCOHOL
0.47 0.09 14
2.37 0.53 14
2 1 14
2.26 0.40 14
0.57 0.18 14
38 4 14
5.90 0.58 14
8.4 1.5 14
64.8 2.8 14
33.8 1.4 14
31.0 2.4 14
1.1 0.1 14
4.04 0.95 14
GROUP 3 TELOMER
ACRYLATE
GROUP 4
0.55 0.14 14
2.24 0.36 14
3 0 14
2.09 0.40 14
0.58 0.14 14
39 4 14
6.14 0.70 14
8.8 1.7 14
65.4 2.5 14
34.1 1.6 14
31.3 1.9 14
1.1 0.1 14
4.52 1.18 14
0.52 0.12 14
2.30 0.35 14
3 1 14
2.32 0.33 14
0.56 0.13 14
41 4 14
6.00 0.50 14
9.0 1.7 14
66.2 2.9 14
33.8 2.2 14
32.4 2.0 14
1.0 0.1 14
4.30 0.87 14
GROUP 5 PFHS
GROUP 6 PFOS
0.57 0.09 14
2.45 0.69 14
2 1 14
1.81 0.28 14
0.51 0.16 14
40 3 14
6.02 0.72 14
8.5 0.9 14
65.4 4.1 14
33.7
1.5 14
31.7 3.1 14
1.1 0.1 14
4.06 1.10 14
0.63 0.32 14
2.32 0.38 14
3 1 14
2.04 0.35 . 14
0.54 0.16 14
41
4
14
5.98 1.02 14
10.2 1.9 14
66.8 3.1 14
34.9 1.7 14
31.8 2.4 14
1.1 0.1 14
4.30 1.14 14
*/**: Ounnett-test based on pooled variance sig. at 5% or 1% level. +: Steel*test s1g. at 5% level.
91
NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/
"PFHS/PFOS
`'
BACK TO MAIN
CHE-SUM - 4 09-N0V-98
CLINICAL BIOCHEMISTRY SUMMARY AFTER 4 WEEKS FEMALES
TREATMENT
GROUP 1 VEHICLE CONTROL
GROUP 2 TELOMER ALCOHOL
INORG PHOSPH MEAN
m m o 1/1
ST.DEV.
N
SODIUM nimol/1
MEAN ST.DEV. N
POTASSIUM mmol/1
MEAN ST.DEV.
N
CALCIUM mmol/1
MEAN ST.DEV. N
CHLORIDE mmol/1
MEAN ST.DEV.
N
'
2.58 0.23 14
142.2 1.6 14
4.13 0.39 14
2.56 0.09 14
97 3 14
2.53 0.16 14
142.6 0.8 14
4.14 0.34 14
2.60 0.06 14
99 2 14
GROUP 3 TELOMER ACRYLATE
GROUP 4
2.47 0.20 14
142.6 1.1 14
4.11 0.32 14
2.60 0.05 14
98 2 14
2.42 0.19 14
142.0 1.0 14
4.19 0.24 14
2.60 0.05 14
99 2 14
GROUP 5 PFHS
2.43 0.21 14
141.8 0.7 14
4.08 0.28 14
2.56 0.06 14
102 ** 2 14
GROUP 6 PFOS
2.59 0.23 14
142.5 1.1 14
4.06 0.30 14
2.60 0.07 14
101 2 4
/**: Dunnett-test based on pooled variance s1g. at 5% or 1% level. +: Steel-test s1g. at 5% level.
NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/
*PFHS/PFOS
"
BACK TO MAIN
CHE-SUM - 5 09-DEC-98
CLINICAL BIOCHEMISTRY SUMMARY AFTER 2 WEEKS RECOVERY MALES
TREATMENT
ALAT(GPT) ukat/1
ASAT(GOT) ukat/1
BILI T. umol/1
CHOLEST.T. mmol/I
TRIGL. mmol/I
CREATININE umol/1
GLUCOSE mmol/I
UREA mmol/1
PROTEIN T. B/1
ALBUMIN g/1
GLOBULIN g/i
A/G RATIO
ALP ukat/1
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
CAN
ST.DEV.
N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
GROUP 1 VEHICLE CONTROL
GROUP 2 . TELOMER ALCOHOL
GROUP 3 TELOMER
ACRYLATE
GROUP 4
GROUP 5 PFHS
GROUP 6 PFOS
0.86 0.19
3
2.80 0.55 3
3 0
3
1.95 0.50 3
0.53 0.09 3
35
8 3
5.65 0.39 3
6.8 1.3 3
55.9 1.5 3
.
29.0 1.0 3
26.9 2.1 3
1.1 0.1 3
6.59 2.07
3
0.62 0.08 3
2.70 0.40 3
3 1 3
1.67 0.69 3
0.61 0.20 3
34 2 3
6.23 1.66 3
7.3 0.5 3
51.5 * 1.0 3
28.1 0.5 3
23.4 0.8 3
1.2 0.0 3
6.93 1.02 3
0.65 0.12 3
2.91 0.03 3
3 1 3
1.57 0.04 3
0.89 0.35 3
33 2 3
6.85 0.93 3
6.9 0.7 3
57.6 0.9 3
29.5 0.3 3
28.1 0.8 3
1.0 0.0 3
5.82 1.22 3
0.85 0.18 3
2.86 0.59 3
3 1 3
1.93 0.43 3
0.70 0.10 3
39 2 3
8.33 * 0.11 3
6.8 0.5 3
53.6 3.2 3
27.8 1.3 3
25.8 2.1 3
1.1 0.1 3
5.94 1.02 3
0.81 0.13 3
2.43 0.42 3
2 0 3
1.38 0.22 3
0.65 0.09 3
39 4 3
7.14 0.16 3
6.7 0.8 3
54.7 0.7 3
28.9 1.0 3
25.8 0.9 3
1.1 0.1 3
6.72 0.80 3
0.64 0.17 3
1.92 0.48 3
3 1 3
1.27 0.19 3
0.50 0.10 3
39 9 3
6.22 1.02 3
7.4 0.7 3
56.5 0.7 3
2 9 .7
0.6 3
26.8 0.5 3
1.1 0.0 .3
5.61 1.21 3
*/**: Dunnett test based on pooled variance s1g. at 5% or 1% level . +: Steel test s1g. at 5% level
93
NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/
*/PFHS/PFOS
"
BACK TO MAIN
CHE-SUM - 6 09-DEC-98
CLINICAL BIOCHEMISTRY SUMMARY AFTER 2 WEEKS RECOVERY MALES ,
TREATMENT
GROUP 1 VEHICLE CONTROL
GROUP 2 TELOMER ALCOHOL
INORG PHOSPH MEAN
mmol/1
ST.DEV.
N
SODIUM mmol/1
MEAN ST.DEV. N
POTASSIUM mmol/1
MEAN ST.DEV. N
CALCIUM mmol/1
MEAN ST.DEV.
N
CHLORIDE mmol/1
MEAN ST.DEV. N
2.81 0.06 3
144.3 1.1 3
4.46 0.42 3
2.48 0.04 3
103 1 3
2.78 0.16 3'
143.4 2.1 3
4.51 0.23 3
2.44 0.06 3
102 3 3
GROUP 3 TELOMER ACRYLATE
GROUP 4
2.74 0.23 3
142.0 0.8 3
4.95 0.13 3
2.48 0.03 3
100 2 3
2.64 0.11 3
141.1 * 0.7 3
4.41 0.20 3
2.50 0.11 3
100 1 3
GROUP 5 PFHS
2.51 0.14 3
142.4 0.9 3
4.56 0.15 3
2.45 0.04 3
102 2 3
GROUP 6 PFOS
2.65 0.12 3
142.8 0.1 3
4.56 0.24 3
2.57 0.07 3
103 2 3
*/**: Dunnett-test based on pooled variance s1g. at 5% or 1% level. +: Steel-test slg. at 5% level.
94
BACK TO MAIN
NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/
'' PFHS/PFOS
CHE-SUM - 7 0 9 -DEC-98
CLINICAL BIOCHEMISTRY SUMMARY AFTER 2 WEEKS RECOVERY FEMALES
TREATMENT
ALAT(GPT) ukat/1
ASAT(GOT) ukat/1
BILI T.
UfROl/1
CHOLEST.T. mmol/l
TRIGL. mmol/l
CREATININE umol/1
GLUCOSE mmol/l
UREA mmol/l
PROTEIN T. 9/1
ALBUMIN
g/i
GLOBULIN gn
A/G RATIO
ALP ukat/1
MEAN ST.DEV. N
MEAN ST.OEV. N
MEAN ST.DEV. N
MEAN ST.OEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN
ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
GROUP 1 VEHICLE CONTROL
0.47 0.05 3
2.53 0.58 3
3 1 3
2.16 0.38 3
0.69 0.24 3
39. 2 3
7.34 1.68 3
6.8 0.7 3
63.6 2.8 3
32.2
2.7 3
31.5 1.2 3
1.0 0.1
3
3.30 0.12 3
GROUP 2 TELOMER ALCOHOL
0.63 0.34 3
2.61 0.69 3
2 1 3
1.92 0.43 3
0:43 0.03 3
40 2 3
6.52 0.66 3
7.3 1.4 3
61.7 3.8 3
3 2 .7
2.8 3
29.0 2.8 3
1.1 0.2 3
3.60 1.10 3
GROUP 3 TELOMER ACRYLATE
GROUP 4
0.49 0.11 3
2.10 0.36 3
2 0 3
1.74 0.40 3
0.61 0.24 3
41 5 3
7.31 0.54 3
8.2 1.1 3
61.1 1.6 3
31.6 1.9 3
29.5 2.3 3
1.1 0.1 3
4.36 1.08 3
0.57 0.08 3
2.53 0.34 3
3 1 3
2.41 0.28 3
0.73 0.26 3
45 7 3
7.41 1.04 3
9.8 * 1.1 3
65.2 3.1 3
33.8 1.7 3
31.4 1.8 .3
1.1 0.0 3
3.85 0.88 3
GROUP 5 PFHS
0.52 0.12 3
1.66 0.15 3
3 0 3
2.11 0.40 3
0.40 0.09 3
44 4 3
7.55 1.70 3
7.6 0.4 3
66.4 4.2 3
31.4 1.0 3
35.1 3.3 3
0.9 0.1 3
3.27 1.20 3
GROUP 6 PFOS
0.45 0.07 3
2.09 0.17 3
2 1 3
2.02 0.37 3
0.31 0.08 3
42 1 3
7.69 0.33 3
8.0 1.2 3
60.6 5.2 3
32.5 1.2 3
28.1 4.0 3
1.2 0.1 3
4.32 1.51 3
*/*: Dunnett -test based on pooled variance sig. at 5% or 1% level . +: Steel -test sig. at 5% level
95
NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/'
"" /PFHS/PFOS
"
BACK TO MAIN
CHE-SUM - 8 09-DEC-98
CLINICAL BIOCHEMISTRY SUMMARY AFTER 2 WEEKS RECOVERY FEMALES
TREATMENT
INORG PHOSPH MEAN
mmol/1
ST.DEV.
N
SODIUM m m o 1/1
MEAN ST.DEV. N
POTASSIUM mmol/I
MEAN ST.DEV. N
CALCIUM mmol/1
MEAN ST.DEV. N
CHLORIDE mmol/1
MEAN ST.DEV.
N
GROUP 1 VEHICLE CONTROL
2.47 0.09 3
142.4 1.0 3
4.08 0.09 3
2.63 0.14 3
99 1 3
GROUP 2 TELOMER ALCOHOL
2.57 0.36 3
142.8 1.3 3
3.96 0.20 3
2.59 0.06 3
99 1 3
GROUP 3 TELOMER ACRYLATE
GROUP 4
2.32 0.06 3
142.0 1.0 3
4.05 0.42 3
2.57 0.09 3
99 4 3
2.55 0.27 3
141.3 1.5 3
4.34 0.38 3
2.68 0.03 3
98 2 3
GROUP 5 PFHS
2.42 0.26 3
141.6 0.8 3
3.90 0.23 3
2.68 0.14 3
98 3 3
GROUP 6 PFOS
2.29 0.10 3
142.5 0.8 3
4.11 0.15 3
2.63 0.07 3
100 2 3
*/*: Dunnett-test based on pooled variance s1g. at 5% or 1% level. +: Steel-test s1g. at 5% level.
96
NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/
-PFHS/PFOS
BACK TO MAIN
CHE-SUM - 9 09-N0V-98
CLINICAL BIOCHEMISTRY SUMMARY AFTER 4 WEEKS RECOVERY MALES
TREATMENT
ALAT(GPT) MEAN
ukat/1
ST.DEV.
N
ASAT(GOT) ukat/1
MEAN
ST.DEV. N
Bill T. umo1/1
MEAN
ST.DEV. N
CHOLEST.T. mmol/1
MEAN
ST.DEV. N
TRIGL. mrnol/1
MEAN ST.DEV. N
CREATININE umol/1
MEAN ST.DEV. N
GLUCOSE nnnol/1
MEAN ST.DEV. N
UREA mmol/I
MEAN ST.DEV. N
PROTEIN T. 0/1
MEAN
ST.DEV. N
ALBUMIN g/1
MEAN ST.DEV.
N
GLOBULIN g/1
MEAN ST.DEV. N
A/G RATIO
MEAN
ST.DEV. N
ALP ukat/1
MEAN
ST.DEV. N
GROUP 1 VEHICLE CONTROL
GROUP 2 TELOMER ALCOHOL
0.59 0.17 3
2.65 0.25 3
3 1 3
1.94 0.18 3
0.76 0.39 3
36 1 3
8.01 0.40 3
6.3 0.3 3
62.2 0.1 3
28.6 0.6 3
33.5 0.6 3
0.9 0.0 3
4.33 0.65 3
0.68 0.07 3
2.44 0.25 3
2 1 3
1.25 0.13 3.
0.58 0.03 3
33 4 3
7.45 1.37 3
6.3 0.2 3
55.9 * 1.8 3
2 7.3
0.5 3
28.6 2.3 3
1.0 . 0.1
3
6.20 1.91 3
GROUP 3 TELOMER ACRYLATE
GROUP 4
GROUP 5 PFHS
0.60 0.03 3
2.06 0.24 3
3 1 3
1.53 0.39 3
0.53 0.11 3
34 4 3
7.06 1.05 3
6.6 0.9 3
55.4 3.5 3
2 7.0
1.4 3
28.4 2.3 3
1.0 0.0 3
5.28 1.46 3
0.58 0.17 3
2.39 0.50 3
3 1 3
1.66 0.35 3
0.56 0.02
3
38 1 3
6.12 0.55 3
7.5
0.7 3
57.0 * 2.3
3
2 7 .7
1.3 3
29.3 1.8
3
0.9 0.1
3
5.43 1.31 3
0.75 0.17 3
2.40 0.20 3
2 1 3
1.24 0.09 3
0.36 0.19 3
43 6 3
5.57 * 0.70 3
8.3 2.0 3
57.3 1.2 3
2 7.3
0.6 3
30.0 1.0 3
0.9 0.0 3
6.00 1.94 3
GROUP 6 PFOS
0.64 0.12 3
1.87 * 0.13 3
2* 0 3
1.34 0.37 3
0.45 0.12 3
32 4 3
6.70 0.62 3
6.0 0.5 3
54.5 ** 2.5 3
2 7 .5
0.7 3
26.9 2.0 3
1.0 0.1 3
4.73 0.19 3
*/**: Dunnett -test based on pooled variance s1g. at 5% or 1% level. +: Steel-test si g. at 5% level
97
NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/
-PFHS/PFOS
"
BACK TO MAIN
CHE-SUM -10 09-N0V-98
CLINICAL BIOCHEMISTRY SUMMARY AFTER 4 WEEKS RECOVERY MALES
TREATMENT
INORG PHOSPH MEAN
m m o 1/1
ST.DEV.
N
SODIUM mmol/1
MEAN ST.DEV.
N
POTASSIUM mmol/1
MEAN ST.DEV. N
CALCIUM mmol/1
MEAN ST.DEV. N
CHLORIDE mmol/1
MEAN ST.DEV. N
GROUP 1 VEHICLE CONTROL
3.02 0.13 3
141.6 0.9 3
5.33 0.21 3
2.58 0.02 3
102 2 3
GROUP 2 TELOMER ALCOHOL
2.64 0.28 3
142.9 1.3 3
4.59 * 0.14 3
2.48 * 0.06 3
103 3 3
GROUP 3 TELOMER ACRYLATE
GROUP 4
2.64 0.24 3
142.5 1.2 3
4.64 * 0.33 3
2.48 * 0.05 3
104 2 3
2.46 * 0.18 3
142.0 0.6 3
4.55 ** 0.12 3
2.46 * 0.03 3
104 1 3
GROUP 5 PFHS
2.65 0.18 3
143.8 1.0 3
4.57 * 0.42 3
2.49 0.02 3
103 1 3
GROUP PFOS
2.59 0.20 3
142.7 0.2 3
4.65 ' 0.11 3
2.48 ' 0.05 3
107 * 2 3
*/**: Dunnett-test based on pooled variance s1g. at 5% or 1% level. +: Steel-test s1g. at 5% level.
98
NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/
*~ I P FHS/PFOS
"
BACK TO MAIN
CHE-SUM -11 09-N0V-98
CLINICAL BIOCHEMISTRY SUMMARY AFTER 4 WEEKS RECOVERY FEMALES
TREATMENT
ALAT(GPT) ukat/1
ASAT(GOT) ukat/1
BILI T. umol/1
CHOLEST.T. mmol/I
TRIGL. mmol/I
CREATININE umol/1
GLUCOSE mmol/1
UREA mmol/1
PROTEIN T.
g/i
ALBUMIN g/1
nGLOBULIN
g
A/G RATIO
ALP ukat/1
MEAN ST.DEV. . N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
GROUP 1 VEHICLE CONTROL
GROUP 2 TELOMER ALCOHOL
GROUP 3 TELOMER ACRYLATE
GROUP 4
GROUP 5 PFHS
GROUP 6 PFOS
0.38 0.09 3
2.25 0.31 3
2 1 3
2.50 0.38 3
0.63 0.13 3
41 3 3
7.16 1.26 3
7.5 1.6 3
64.9 4.1 3
31.3 1.7 3
33.6 2.5 3
0.9 0.0 3
2.95 0.46 3
0.37 0.04 2'
2.31 0.33 3
2 1 3
2.41 0.36 3
0.74 0.27 3
42 5 3
6.43 0.47 3
7.5 1.3 3
64.3 2.5 3
31.6 1.2 3
32.6 1.9 3
1.0 0.1 3
3.03 1.24 3
0.46 . 0.12
3
1.89 0.45 3
3 1 3
2.30 0.11 3
0.47 0.04 3
42 3 3
6.90 0.37 3
8.0 1.0 3
67.7 2.2 3
33.4 3.4 3
34.4 1.5 3
1.0 0.1 3
3.11 0.92 3
0.63 0.31 3
2.45 0.26 3
2 0 3
2.51 0.49 3
0.51 0.18 3
.
44 4 3
6.34
1.56 3
8.3 1.5 3
65.3 3.4
3
30.6
1.3 3
34.7 2.1 3
0.9 0.0
3
2.93 0.85 3
0.47 0.16 3
2.52 0.49 3
2 0 3
2.06 0.35 3
0.40 0.06 3
39 1 3
6.86 0.41 3
7.0 0.3 3
69.4 0.9 3
34.2 0.7 3
35.2 1.4 3
1.0 0.1 3
2.70 0.85 3
0.50 0.02 3
1.92 0.05 3
2 1 3
2.13 0.22 3
0.50 0.21 3
39 1 3
7.65 1.09 3
10.7 3.7 3
65.3 5.4 3
32.8 2.0 3
32.5 3.3 3
1.0 0.0 3
3.38 0.19 3
*/**: Dunnett -test based on pooled variance s1g. at 5% or 1% level. +: Steel-test sig. at 5% level
99
NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/
*PFHS/PFOS
BACK TO MAIN
CHE-SUM -12 O9-N0V-98
CLINICAL BIOCHEMISTRY S U M M A R Y AFTER 4 WEEKS RECOVERY FEMALES
TREATMENT
IN0R6 PHOSPH MEAN
mmol/I
ST.DEV.
N
SODIUM mmol/I
MEAN ST.DEV.
N
POTASSIUM mmol/I
MEAN ST.DEV. N
CALCIUM mmol/I
MEAN ST.DEV. N
CHLORIDE. mmol/I
MEAN ST.DEV. N
GROUP 1 VEHICLE CONTROL
2.33 0.17 3
142.3 1.8 3
4.12 0.34 3
2.52 0.05 3
100 4 3
GROUP 2 TELOMER ALCOHOL
2'24 0.12 3
141.6 0.5 3
4.14 0.29 3
2.53 0.10 3
101 3 3
GROUP 3 TELOMER
ACRYLATE
GROUP 4
1.98 0.20 3
140.7 1.7 3
4.48 0.89 3
2.55 0.07 3
100 4 3
2.22 0.30 3
141.3 0.7 3
4.26 0.51 3
2.57 0.04 3
99 3 3
GROUP 5 PFHS
GROUP 6 PFOS
2.16 0.08 3
141.0 1.4 3
4.02 0.33 3
2.60 0.01 3
102 3 3
2.27 0.26 3
141.1 1.6 3
4.16 0.18 3
2.64 0.03 3
103 5 3
*/'*: Dunnett-test based on pooled variance s1g. at 5% or 1% level. +: Steel-test sig. at 5% level. 1 00
NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/
'' PFHS/PFOS
"
BACK TO MAIN
OW-SUM - l 08-DEC-98
ORGAN WEIGHTS (GRAM) SUMMARY AFTER 4 WEEKS MALES
TREATMENT
BODY W. BRAIN HEART LIVER KIDNEYS ADRENALS SPLEEN TESTES
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST. DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
GROUP 1 VEHICLE CONTROL
GROUP 2 TELOMER ALCOHOL
315 315 30 16 8 8'
2.01 0.07 8
1.96 0.07 8
1.133
1.134
0.108 0.144
88
10.05 1.24 8
10.35 0.68 8
2.30 0.36
8
2.34 0.18 8
0.045 0.006
8
0.053
0.008 8
0.580
0.119 8
0.566
0.069 8
2.87 0.23 8
3.05 0.22 8
GROUP 3 TELOMER ACRYLATE
GROUP 4
305 13 8
1.95 0.06 8
1.097 0.115 8
9.36 0.60 8
2.20 0.15 8
0.052 0.007 8
0.567 0.065 8
2.78 0.15 8
306 27 8
2.00 0.06 8
1.154 0.106 8
9.04 1.08 8
2.16 0.25 8
0.046 0.008 8
0.578 0.075 8
2.86 0.19 8
GROUP 5 PFHS
GROUP 6 PFOS
308 28 8
1.96 0.09 8
1.107 0.088 8
13.78 ** 1.83 8
2.34 0.22 8
0.050 0.009 8
0.570 0.076 8
2.80 0.80 8
318 25 8
2.00 0.05 8
-
1.131 0.103 8
11.79 * 1.00 8
2.19 0.18 8
0.046 0.013 8
0.558 0.096 8
2.85 0.18 8
*/**: Dunnett-test based on pooled variance sig. at 5% or 1% level.
NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/
'PFHS/PFOS
"
BACK TO MAIN
OW-SUM - 2 08-DEC-98
ORGAN/BODY WEIGHT RATIOS SUMMARY AFTER 4 WEEKS MALES
TREATMENT
BODY W. (GRAM)
BRAIN (%)
HEART (%)
LIVER (%)
KIDNEYS (%)
ADRENALS (%)
SPLEEN (%)
TESTES (%)
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST. DEV. N
MEAN ST.DEV. N
GROUP 1 VEHICLE CONTROL
315 30 8
0.64 0.05 8
0.362 0.037 a
3.19 0.21 8
0.73 0.09 8
0.014 0.002 8
0.183 0.027 8
0.91 0.05 8
GROUP 2 TELOMER ALCOHOL
315 16 8
0.62 0.03 8
0.361 0.046 8
3.30 0.29 8
0.74 0.06 8
0.017 0.003 8
0.180 0.017 8
0.97 0.09 8
GROUP 3 TELOMER ACRYLATE
GROUP 4
305 13 8
0.64 0.03 8
0.360 0.037 8
3.07 0.11 8
0.72 0.05 8
0.017 0.002 8
0.186 0.015 8
0.91 0.06 8
306 27 8.
0.66 0.05 8
0.378 0.026 8
2.95 0.14 8
0.70 0.03 8
0.015 0.003 8
0.189 0.018 8
0.94 0.12 8
GROUP 5 PFHS
308 28 8
0.64 0.05 8
0.36a 0.023 8
4.47 * 0.40 8
0.76 0.05 8
0.016 0.003 8
0.185 0.016 8
0.90 0.24 8
GROUP 6 PFOS
318 25 8
0.63 0.05 8
0.357 0.041 8
3.71 ** 0.30 8
0.69 0.07 8
0.014 0.003 8
0.175 0.026 8
0.90 0.05 8
*/**: Dunnett-test based on pooled variance s1g. at 5% or 1% level.
1 02
NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/
*. PFHS/PFOS
BACK TO MAIN
OW-SUM - 3 0 8 -DEC-98
ORGAN WEIGHTS (GRAM) SUMMARY AFTER 4 WEEKS FEMALES
TREATMENT
BODY W. BRAIN HEART LIVER KIDNEYS ADRENALS SPLEEN OVARIES
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
GROUP 1 - VEHICLE
CONTROL
214 22 8
1.88 0.08 8
0.852 0.054 8
6.62 0.50 8
1.50 0.15 8
0.060 0.009 8
0.423 0.051 8
0.124 0.023 8
GROUP 2 TELOMER ALCOHOL
211 16 8'
1.93 0.08 8
0.813 0.054 8
7.18 0.55 8
1.63 0.15 8
0.064 0.009 8
0.450 0.037 8
0.133 0.020 8
GROUP 3 TELOMER ACRYLATE
GROUP 4
200 16 8
1.85 0.05 7
0.790 0.071 7
6.37 0.47 7
1.51 0.11 7
0.060 0.009 7
0.417 0.086 7
0.135 0.015 7
208 15 8
1.90 0.08 8
0.805 0.073 8
6.53 0.72 8
1.53 0.17 8
0.070 0.037 8
0.608 0.373 8
0.124 0.030 8
GROUP 5 PFHS
201 13 8
1.88 0.09 8
0.806 0.083 8
6.71 0.70 8
1.51 0.12 8
0.058 0.009 8
0.407 0.033 8
0.132 0.018 8
GROUP 6 PFOS
187 * 16 8
1.88 0.05 8
0.748 * 0.073 8
7.80 ** 0.88 8
1.46 0.12 8
0.054 0.006 8
0.412 0.046 8
0.128 0.019 8
*/**: Dunnett-test based on pooled variance s1g. at 5% or 1% level.
NOTOX Project 242933
'-
TELOMER ALCOHOL/TELOMER ACRYLATE/ ... PFHS/PFOS
"
BACK TO MAIN
OW-SUM - 4 08-DEC-98
ORGAN/BODY WEIGHT RATIOS SUMMARY AFTER 4 WEEKS FEMALES
TREATMENT
BODY W. (GRAM)
BRAIN (%)
HEART (%)
LIVER (%)
KIDNEYS (%)
ADRENALS (%)
SPLEEN (%)
OVARIES (%)
MEAN ST.DEV. N
MEAN ST. DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N.
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
GROUP 1 VEHICLE CONTROL
214 22 8
0.89 0.08 8
0.401 0.035 8
3.11 0.19 8
0.70 0.03 8
0.028 0.006 8
0.198 0.024 8
0.058 0.010 8
GROUP 2 TELOMER ALCOHOL
GROUP 3 TELOMER ACRYLATE
GROUP 4
GROUP 5 PFHS
211 16 8
0.92 0.05 8
0.385 0.027 8
3.40 0.13 B
0.77 0.07 8
0.031 0.005 8
0.214 0.020 8
0.063 0.012 8
200 16 8
0.93 0.06 .7
0.395 0.023 7
3.19 0.13 7
0.76 0.04 7
0.030 0.002 7
0.208 0.037 7
0.068 0.008 7
208 15 8
0.91 0.04 8
0.388 0.030 8
3.14 0.28 8
0.74 0.08 8
0.033 0.016 8
0.298 0.201 8
0.060 0.015 8
201 13 8
0.94 0.05 .8
0.402 0.034 8
3.35 0.35 8
0.75 0.06 8
0.029 0.005 8
0.204 0.016 8
0.066 0.008 8
GROUP 6 PFOS
187 * 16 8
1.01 0.07 8
0.399 0.028 8
4.15 ` 0.18 8
0.78 * 0.03 8
0.029 0.003 8
0.220 0.013 8
0.069 0.013 8
/**: Dunnett-test based on pooled variance s1g. at 5% or 1% level.
NOTOX Project 242933
'"
TELOMER ALCOHOL/TELOMER ACRYLATE/ .,/PFHS/PFOS
BACK TO MAIN
OW-SUM - 5 08-DEC-98
ORGAN WEIGHTS (GRAM) SUMMARY AFTER 2 WEEKS RECOVERY MALES
TREATMENT
BODY W. BRAIN HEART LIVER KIDNEYS ADRENALS SPLEEN TESTES
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
GROUP 1 VEHICLE CONTROL
320 37 3
1.95 0.12 3
1.091 0.117 3
8.24 0.78 3
2.04 0.31 3
0.042 0.007 3
0.441 0.084 3
2.81 0.24 3
GROUP 2 TELOMER ALCOHOL
314 17 3
1.98 0.07 3
1.062 0.053 3
8.27 0.63 3
2.08 0.03 3
0.039 0.008 3
0.568 0.012 3
2.13 0.89 3
GROUP 3 TELOMER ACRYLATE
GROUP 4
344 31 3
1.95 0.13 3
1.172 0.097 3
9.89 1.91 3
2.41 0.31 3
0.043 0.006 3
0.543 0.037 3
3.09 0.16 3
366 44 3
2.02 0.11 3
1.153 0.027 3
10.30 1.19 3
2.39 0.25 3
0.055 0.012 3
0.623 0.102 3
3.35 0.17 3
GROUP 5 PFHS
324 69 3
1.93 0.18 3
1.149 0.145 3
9.55 2.63 3
2.18 0.51 3
0.041 0.005 3
0.541 0.080 3
2.61 1.09 3
GROUP 6 PFQS
380 12 3
2.03 0.03 3
1.299 0.054 3
14.07 0.63 3
2.65 0.06 3
0.048 0.007 3
0.635 0.121 3
3.03 0.26 3
/**: Dunnett-test based on pooled variance s1g. at 5% or 1% level.
NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/
'/PFHS/PFOS
`'
BACK TO MAIN
OW-SUM - 6 08-DEC-98
ORGAN/BODY WEIGHT RATIOS SUMMARY AFTER 2 WEEKS RECOVERY MALES
TREATMENT
BODY W. (GRAM)
BRAIN (%)
HEART (%)
LIVER (%)
KIDNEYS (%)
ADRENALS (%)
SPLEEN (%)
TESTES {%)
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
GROUP 1 VEHICLE CONTROL
320 37 3
0.61 0.04 3
0.342 0.007 3
2.58 0.11 3
0.64 0.06 3
0.013 0.002 3
0.138 0.016 3'
0.88 0.04 3
GROUP 2 TELOMER ALCOHOL
314 17 3'
0.63 0.04 3
0.339 0.029 3
2.63 0.07 3
0.66 0.05 3
0.013 0.003 3
0.181 0.011 3
0.68 0.30 3
GROUP 3 TELOMER
ACRYLATE
GROUP a
344 31 3
0.57 0.06 3
0.344 0.055 3
2.86 0.30 3
0.70 0.03 3
0.013 . 0.001 3
0.159 0.024 3
0.90 0.09 3
366 44 3
0.55 0.04 3
0.318 0.042 3
2.82 0.12 3
0.65 . 0.05 3
0.016 0.005 3
0.170 0.007 3
0.92 0.06 3
GROUP 5 PFHS
324 69 3
0.61 0.09 3
0.361 0.045 3
2.93 0.20 3
0.67 0.04 3
0.013 0.001 3
0.169 0.019 3
0.78 0.22 3
GROUP 6 PFOS
380 12
3
0.53 0.02 3
0.342
0.009 3
3.70 ** 0.22 3
0.70 0.02 3
0.013 0.002 3
0.167 0.028
3
0.80 0.04
3
*/**: Dunnett-test based on pooled variance s1g. at 5% or 1% level.
1 06
NOTOX Project 242933
*-
TELOMER ALCOHOL/TELOMER ACRYLATE/ ..... PFHS/PFOS
"
BACK TO MAIN
OW-SUM - 7 08-DEC-98
ORGAN WEIGHTS (GRAM) SUMMARY AFTER 2 WEEKS RECOVERY FEMALES
TREATMENT
BODY W. BRAIN HEART LIVER KIDNEYS ADRENALS SPLEEN OVARIES
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
GROUP 1 VEHICLE CONTROL
239 29 3
1.96 0.10 3
0.898 0.053 3
7.69 1.33 3
1.54 0.16 3
0.055 0.003 3
0.420 0.042 3
0.285 0.250 3
GROUP 2 TELOMER ALCOHOL
211 17 3
1.89 0.07 3
0.858 0.052 3
6.82 0.86 3
1.51 0.17 3
0.063 0.010 3
0.424 0.097 3
0.130 0.005 3
GROUP 3 TELOMER ACRYLATE
GROUP 4 . GROUP 5 PFHS
202 14 3
1.91 0.03 3
0.831 0.056 3
6.38 0.91 3
1.52 0.16 3
0.065 0.017 3
0.416 0.094 3
0.126 0.010 3
217 28 3
1.91 0.03 3
0.936 0.052 3
7.07 0.09 3
1.60 0.12 3
0.069 0.005 3
0.414 0.016 3
0.145 0.020 3
224 11 3
1.92 0.02 3
0.840 0.054 3
7.19 0.67 3
1.51 0.06 3
0.058 0.003 3
0.449 0.077 3
0.133 0.015 3
GROUP 6 PFOS
200 3 3
1.99 0.06 3
0.723 ** 0.018 3
7.55 0.56 3
1.38 0.11 3
0.056 0.001 3
0.392 0.025 3
0.132 0.006 3
/**: Dunnett-test based on pooled variance s1g. at 5% or 1% level.
BACK TO MAIN
NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE,
"PFHS/PFOS
"
OW-SUM - 8 08-DEC-98
ORGAN/BODY WEIGHT RATIOS SUMMARY AFTER 2 WEEKS RECOVERY FEMALES
TREATMENT
BODY W. (GRAM)
MEAN ST.DEV. N
BRAIN (%>
' MEAN ST.DEV. N
HEART (%)
MEAN ST.DEV. N
LIVER (%)
MEAN ST.DEV.
N
KIDNEYS (%)
MEAN ST.DEV.
N
ADRENALS (%)
MEAN ST.DEV. N
SPLEEN (*)
MEAN ST.DEV. N
OVARIES l*)
MEAN ST.DEV. N
GROUP 1 VEHICLE CONTROL
239 29 3
0.83 0.08 3
0.378 0.024 3
3.21 0.18 3
0.65 0.04 3
0.023 0.002 3
0.177 0.026 3
0.113 0.085 3
GROUP 2 TELOMER ALCOHOL
211 17 3'
0.90 0.04 3
0.408 0.042 3
3.22 0.23 3
0.72 0.08 3
0.030 0.006 3
0.199 0.028 3
0.062 0.005 3
GROUP 3 TELOMER
ACRYLATE
GROUP 4
202 14 3
0.95 0.07 3
0.412 0.013 3
3.15 0.23 3
0.75 0.03 3
0.032 0.006 3
0.205 0.035 3
0.063 0.007 3
. 217 28 3
0.89 0.10 3
0.435 0.042 3
3.30 0.45 3
0.74 0.04 3
0.032 0.005 3
0.192 0.018 3
0.067 0.003 3
GROUP 5 PFHS
224 11 3
0.86 0.04 3
0.375 0.008 3
3.21 0.23 3
0.68 0.06 3
0.026 0.001 3
0.202 0.041 3
0.059 0.004 3
GROUP 6 PFOS
200 3 3
1.00 0.03 3
0.362 0.007 3
3.78 0.32 3
0.69 0.06 3
0.028 0.001 3
0.197 0.015 3
0.066 0.004 3
*/**: Dunnett-test based on pooled variance s1g. at 5% or 1% level.
1 08
NOTOX Project 242933
'-
TELOMER ALCOHOL/TELOMER ACRYLATE/ .'PFHS/PFOS
"
BACK TO MAIN
OW-SUM - 9 08-DEC-98
ORGAN WEIGHTS (GRAM) SUMMARY AFTER 4 WEEKS RECOVERY MALES
TREATMENT
BODY W. BRAIN HEART LIVER KIDNEYS ADRENALS SPLEEN TESTES
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST. DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
GROUP 1 VEHICLE CONTROL
389 28 3
1.97 0.15 3
1.196 0.093 3
10.77 1.37 3
2.44 0.23 3
0.046 0.011 3
0.614 0.040 3
2.86 0.14 3
GROUP 2 TELOMER ALCOHOL
363 33 3'
2.05 0.04 3
1.207 0.018 3
9.78 0.44 3
2.42 0.06 3
0.050 0.009 3
0.540 0.082 3
3.19 0.43 3
GROUP 3 TELOMER ACRYLATE
GROUP 4
378 3 3
2.09 0.03 3
1.217 0.077 3
10.30 0.69 3
2.51 0.04 3
0.054 0.004 3
0.591 0.061 3
3.11 0.06 3
401 36 3
2.11 0.19 3
1.299 0.147 3
10.06 0.35 3
2.59 0.24 3
0.056 0.013 3
0.720 0.037 3
3.10 0.22 3
GROUP 5 PFHS
335 20 3
2.02 0.08 3
1.048 0.153 3
8.84 0.63 3
2.24 0.41 3
0.047 0.003 3
0.552 0.065 3
3.09 0.11 3
GROUP 6 PFOS
344 38 3
2.00 0.07 3
1.231 0.248 3
11.17 1.94 3
2.48 0.21 3
0.046 0.009 3
0.629 0.136 3
3.27 0.30 3
*/**: Ounnett-test based on pooled variance s1g. at 5% or 1% level.
NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/
*/PFHS/PFOS
"
BACK TO MAIN
OW-SUM - 10 08-DEC-98
ORGAN/BODY WEIGHT RATIOS SUMMARY AFTER 4 WEEKS RECOVERY MALES
TREATMENT
GROUP 1 VEHICLE CONTROL
BODY W. (GRAM)
BRAIN (%)
HEART (%)
LIVER (%)
KIDNEYS (%)
ADRENALS (%)
SPLEEN (%)
TESTES (%)
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
389 28 3
0.51 0.03 3
0.308 0.027 3
2.76 0.17 3
0.63 0.05 3
0.012 0.003 3
0.159 0.017 3
0.74 0.04 3
GROUP 2 TELOMER ALCOHOL
363 33 3
0.57 0.04 3
0.335 0.030 3
2.70 0.13 3
0.67 0.04 3
0.014 0.002 3
0.149 0.019 3
0.88 0.14 3
GROUP 3 TELOMER ACRYLATE
GROUP 4
378 3 3
0.55 0.01 3
0.322 0.018 3
2.72 0.18 3
0.66 0.01 3
0.014 0.001 3
0.156 0.016 3
0.82 0.02 3
401 36 3
0.53 0.03 3
0.324 0.016 3
2.52 0.21 3
0.65 0.08 3
0.014 0.002 3
0.181 0.026 3
0.78 0.05 3
GROUP 5 PFHS
335 20 3
0.60 * 0.01 3
0.313 0.042 3
2.64 0.03 3
0.67 0.14 3
0.014 0.000 3
0.164 0.010 3
0.92 * 0.02 3
GROUP 6 PFOS
344 38 3
0.59 0.05 3
0.356 0.035 3
3.23 * 0.22 3
0.72 0.02 3
0.014 0.004 3
0.182 0.022 3
0.95 * 0.08 3
*/**: Dunnett-test based on pooled variance s1g. at 5% or 1% level.
110
NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/
-/PFHS/PFOS
''
BACK TO MAIN
OW-SUM - 11 08-DEC-98
ORGAN WEIGHTS (GRAM) SUMMARY AFTER 4 WEEKS RECOVERY FEMALES
TREATMENT
BODY W. BRAIN HEART LIVER KIDNEYS ADRENALS SPLEEN OVARIES
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV-. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
GROUP 1 VEHICLE CONTROL
243 27 3
1.91 0.09 3
0.916 0.118 3
7.01 1.14 3
1.60 0.25 3
0.065 0.012 3
0.445 0.056 3
0.133 0.013 3
GROUP 2 TELOMER ALCOHOL
228 11 3'
1.97 0.06 3
0.851 0.109 3
6.87 0.57 3
1.63 0.09 3
0.064 0.013 3
0.443 0.140 3
0.146 0.029 3
GROUP 3 TELOMER ACRYLATE
GROUP 4
226 25 3
1.88 0.06 3
0.870 0.046 3
6.85 0.86 3
1.54 0.14 3
0.062 0.008 3
0.426 0.065 3
0.145 0.013 3
235 35 3
1.96 0.05 3
0.918 0.092 3
7.30 1.56 3
1.68 0.25 3
0.061 0.001 3
0.504 0.104 3
0.165 0.040 3
GROUP 5 PFHS
226 13 3
1.88 0.08 3
0.860 0.065 3
6.83 0.20 3
1.62 0.12 3
0.065 0.003 3
0.355 0.024 3
0.153 0.047 3
GROUP 6 PFOS
208 11 3
1.88 0.09 3
0.825 0.028 3
7.49 0.30 3
1.68 0.12 3
0.058 0.010 3
0.427 0.060 3
0.141 0.003 3
/**: Dunnett-test based on pooled variance s1g. at 5% or 1% level.
NOTOX Project 242933
*-
TELOMER ALCOHOL/TELOMER ACRYLATE/ .... PFHS/PFOS
BACK TO MAIN
OW-SUM - 12 0 8 -DEC-98
ORGAN/BODY WEIGHT RATIOS SUMMARY AFTER 4 WEEKS RECOVERY FEMALES
TREATMENT
BODY W. (GRAM)
BRAIN (%)
HEART (*)
LIVER (%)
KIDNEYS (%)
ADRENALS (%)
SPLEEN (%)
OVARIES (%)
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
MEAN ST.DEV. N
GROUP 1 VEHICLE CONTROL
243 27 3
0.79 0.08 3
0.379 0.039 3
2.88 0.20 3
0.66 0.04 3
0.027 0.005 3
0.184 0.023 3
0.055 0.009 3
GROUP 2 TELOMER ALCOHOL
228 11 3
0.86 0.03 3
0.372 0.029 3
3.02 0.21 3
0.72 0.07 3
0.028 0.006 3
0.193 0.052 3
0.064 0.010 3
GROUP 3 TELOMER ACRYLATE
GROUP 4
226 25 3
0.84 0.06 3
0.387 0.021 3
3.03 0.14 3
0.69 0.07 3
0.028 0.004 3
0.192 0.046 3
0.065 0.011 3
235 35 3
0.85 0.12 3
0.392 0.021 3
3.09 0.29 3
0.71 0.05 3
0.026 0.003 3
0.216 0.048 3
0.070 0.012 3
GROUP 5 PFHS
GROUP 6 PFOS
226 13 3
0.83 0.01 3
0.380 0.017 3
3.02 0.17 3
0.72 0.08 3
0.029 0.001 3
0.157 0.019 3
0.068 0.023 3
208 11 3
0.90 0.04 3
0.398 .0.032 .3
3.61 ** 0.20 3
0.81 * 0.03 3
0.028 0.006 3
0.206 0.031 3
0.068 0.002 3
/*: Dunnett-test based on pooled variance s1g. at 5% or 1% level.
NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/
HISTORICAL DATA HAEMATOLOGY 1 ANIMALS OF 10*12 WEEKS OF AGE MALES AND FEMALES
PFHS/PFOS
PARAMETER SEX
N
MEAN
ST. DEV. MIN.
RBC M T/l F
HB mmol/1
M F
HCT M 1/1 F
MCV fl
M F
MCH fmol
Mriir mmol/l
M F
M F
PLATELETS M G/l F
RDW M %F
WBC M G/l F
SEG l(rel)
M F
EO l(rel)
M F
BASO l(rel)
M F
LYMPH l(rel)
M F
MONO l(rel)
M F
PT M sec F
PTT M sec F
50 44
50 44
' 50 44
50 44
50 44
50 44
50 44
50 44
50 44
50 44
50 44
50 44
50 44
50 44
44 37
44 36
7.08 6.63
9.8 9.5
0.415 0.386
58.6 58.3
1.387 1.441
23.7 24.7
1137 1114
12.2 11.7
11.8 9.2
0.11 0.097
0.006 0.008
0 0
0.855 0.87
0.028 0.025
13.2 13.9
16.5 15.5
0.36 0.32
6.42 6
0.5 8.8 0.3 8.9
0.019 . 0.384
0.016
0.353
1.8 55 1.4 55.4
0.073 0.055
1.245 1.345
1.1 21.1 0.6 23.5
148 760 139 753
0.7 11.1 0.6 10.6
2.7 4.7 2.6 5.3
0.049 0.065
0.015 0.02
0.007 0.008
0 0
00 00
0.061 0.07
0.705 0.705
0.021 0.020
0 0
1 11.2 0.9 12
1.8 11.9 1.9 10.3
.
MAX.
7.95 7.5
10.8 10.4
0.463 0.437
63.6 60.7
1.52 1.584
25.5 26.3
1486 1513
14.4 13.1
17.5 18.7
0.235 0.27
0.02 0.03
0 0
0.985 0.965
0.085 0.075
15.5 15.7
19.7 19.4
BACK TO MAIN
HIS-SUM - 1 22-APR-99
1 Data are from Sprague Oawley control rats which received the vehicle (in general 0.9% NaCl) via continuous Intravenous Infusion. Due to the limited availability of data no confidence Intervals were calculated.
113
NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/
HISTORICAL DATA HAEMATOLOGY1 ANIMALS OF 13-18 WEEKS OF AGE MALES AND FEMALES
PFHS/PFOS
PARAMETER SEX
N
MEAN
ST. DEV. MIN.
RBC M T/l F
HB mmol/1
M F
HCT M 1/1 F
MCV M fl F
MCH fmol
M F
MCHC mmol/1
M F
PLATELETS M G/l F
RDW M %F
WBC M G/l F
SEG l(rel)
M F
EO l(rel)
M F
BASO l(rel)
LYMPH l(rel)
M F
M F
MONO l(rel)
M F
PT M sec F
PTT M sec F
42 30
42 30
42 30
42 30
42 30
42 30
42 30
42 30
42 30
42 30
42 30
42 30
42 30
42 30
38 29
38 29
7.34 6.81
9.3 8.8
0.399 0.376
54 55.2
1.251 1.3
23.1 23.6
1096 1130
14.6 12.9
14.2 8.4
0.157 0.148
0.009 0.008
0 0
0.803 0.823
0.031 0.020
14.2 13.8
16.4 16.3
0.73 0.47
1.3 0.6
0.047 0.027
2.9 2.1
0.087 0.054
0.8 0.5
170 145
1.8 0.8
5.6 2
0.093 0.102
0.009 0.008
0 0
0.108 0.105
0.025 0.016
1.3 1
2.1 1.9
5.59 5.59
5.6 7
0.264 0.313
45.3 51.5
0.961 1.19
21.2 22.4
669 855
12.7 11.6
6.1 4.7
0.04 0.04
0 0
0 0
0.45 0.54
0 0
11.9 11.9
11.6 12.3
MAX.
8.7 7.65
11 9.9
0.472 0.423
59.6 59.8
1.391 1.42
24.4 24.6
1455 1515
20.5 15.2
33.9 12
0.505 0.42
0.035 0.03
0 0
0.935 0.945
0.105 0.065
17.3 15.6
21.2 19.6
BACK TO MAIN
HIS-SUM - 2 22-APR-99
1 Data are from Sprague Dawley control rats which received the vehicle (1n general 0.9% NaCl) via continuous intravenous Infusion. Due to the limited availability of data no confidence Intervals were calculated.
114
NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE
PFHS/PFOS
HISTORICAL DATA CLINICAL BIOCHEMISTRY1 ANIMALS OF 10-12 WEEKS OF AGE MALES AND FEMALES
.
PARAMETER
SEX N
MEAN
ST. DEV. MIN.
ALAT/GPT jikat/1
ASAT/GOT pleat/1
BILI T. pmol/1
CHOLEST. T. mmol/1
TRIGL. mmol/1
CREATININE pmol/1
GLUCOSE mmol/1
UREA mraol/1
PROTEIN T. 0/1
ALBUMIN o/i
GLOBULIN 8/1
A/G RATIO 1
ALP pkat/1
SODIUM mmol/1
POTASSIUM mmol/1
CHLORIDE mmol/1
M F
M F
M F
M F
M F
M F
M F
M F
M F
M F
M F
M F
M F
M F
M F
M F
51 45
51 45
51 45
51 45
51 45
51 45
51 45
51 45
51 45
51 45
51 45
51 45
51 45
51
51 45
51 45
0.66 0.54
2.51 2.46
2 3
1.95 2.05
0.69 0.42
33 40
6.03 5.32
6.1 8.1
64.1 67.4
29.5 32.6
34.6 34.8
0.9 1
7.38 4.3
143.3 141.2
4.83 4.59
100 101
0.11 0.15
0.6 0.43
1 1
0.34 . 0.4
0.37 0.09
6 6
1.07 0.68
1.6 2.1
2.9 4.2
1.8 2
2.5 4.5
0.1 0.1
1.87 1.19
1.6 1.6
0.43 0.3
3 3
0.45 0.24
1.54 1.69
1 2
1.25 1.08
0.34 0.26
25 31
3.86 3.87
3 4.5
57.7 59.5
24.5 28.4
29.1 25.8
0.6 0.7
2.94 2.63
138.5 137.5
3.35 3.97
95 94
.
MAX.
0.98 1.06
3.8 3.48
4 5
2.98 3.31
2.11 0.68
54 56
8.45 7.4
8.9 12.8
70.9 75.5
32.8 36.6
40.9 43.7
1 1.3
12.3 7.86
146.3 144.8
5.97 5.32
106 110
BACK TO MAIN
HIS-SUM - 3 22-APR-99
1 Data are from Sprague Dawley control rats which received the vehicle (1n general 0.9% NaCl) via continuous intravenous Infusion. Due to the limited availability of data no confidence Intervals were calculated.
NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE
PFHS/PFOS
HISTORICAL DATA CLINICAL BIOCHEMISTRY1 ANIMALS OF 10-12 WEEKS OF AGE MALES AND FEMALES
PARAMETER
SEX N
CALCIUM mmol/I
M F
INORG. PHOSPH. M
mmol/1
F
51 45
51 45
MEAN
2.6 2.67
2.78 2.55
ST. DEV. MIN.
0.09 0.09
.21 0.25
2.41 2.46
2.29 2.09
.
MAX.
2.8 2.86 3.27 3.38
BACK TO MAIN
HIS-SUM - 4 2 2 -APR-99
1 Data are from Sprague Dawley control rats which received the vehicle (1n general 0.9% NaCl) via continuous Intravenous Infusion. Due to the limited availability of data no confidence Intervals were calculated.
116
NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/
/PFHS/PFOS
HISTORICAL DATA CLINICAL BIOCHEMISTRY1 ANIMALS OF 13-18 WEEKS OF AGE MALES AND FEMALES
PARAMETER
SEX N
ALAT/GPT pkat/1
M F
42 28
ASAT/GOT pkat/1
M F
42 28
BILI T. pmol/1
M 42 F 28
CHOLEST. T. mmol/1
M F
42 28
TRIGL. mmol/1
M 42 F 28
CREATININE Mmol/1
M 41 F 28
GLUCOSE mmol/1
M 42 F 28
UREA mmol/1
M 46 F 32
PROTEIN T. 9/1
ALBUMIN 9/1
GLOBULIN 9/1
A/G RATIO 1
M F
M F
M F
M F
42 28
42 28
42 28
42 28
ALP pkat/1
M 42 F 28
SODIUM mmol/1
M 42 F 28
POTASSIUM mmol/1
M F
42 . 28
CHLORIDE mmol/1
M F
42 28
MEAN
ST. DEV. MIN.
0.77 0.64
2.64 2.31
2 3
1.93 2.49
0.58 0.47
37 42
6.38 5.93
5.5 5.7
66,4 72.5
28.1 32
38.2 40.5
0.7 0.8
4.21 3.32
142 140
5 4.43
101 103
0.38 0.19
0.55 0.42
1 1
0.43 0.45
0.25 0.19
4 5
1.22 0.71
1.4 1.5
3.8 3.5
3 3.8
4.2 4.4
0.1 0.2
1.04 2.51
2.4 2.7
0.36 0.29
3 3
0.47 0.33
1.65 1.45
1 2
0.98 1.69
0.23 0.27
27 35
4.21 5.04
3 3.7
56 66.8
21 23.9
31 34.4
0.4 0.5
2.45 1.15
137.1 135.8
4.38 3.87
96 98
MAX.
2.98 1.15
4.05 3.09
4 5
2.8 3.27
1.13 1.24
46 52
10.65 7.43
8.5 9.6
74 81
32.8 38.2
49 50.8
1 1.1
7.55 14.54
148.3 147.1
5.81 5.19
107 110
BACK TO MAIN
HIS-SUM 5 22-APR-99
1 Data are from Sprague Dawley control rats which received the vehicle (In general 0.9% NaCl) via continuous intravenous Infusion. Due to the limited availability of data no confidence Intervals were calculated.
117
NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE
PFHS/PFOS
HISTORICAL DATA CLINICAL BIOCHEMISTRY1 ANIMALS OF 13-18 WEEKS OF AGE MALES AND FEMALES
PARAMETER
SEX N
CALCIUM mmol/1
M F
INORG. PHOSPH. M
mmol/1
F
42 28
42 28
MEAN
2.53 2.5
2.6 2.1
ST. DEV. MIN.
0.14 0.17
0.24 0.23
2.23 2.26
2.17 1.65
MAX.
2.78 2.8
3.34 2.56
BACK TO MAIN
HIS-SUM - 6 22-APR-99
1 Data are from Sprague Dawley control rats which received the vehicle (1n general 0.9% NaCl) via
continuous Intravenous Infusion. Due to the limited availability of data no confidence Intervals were
calculated.
.
118
TELOMER ALCOHOL/TELOMER ACRYLATE/ VPFHS/PFOS
BACK TO MAIN
NOTOX 242933
TABLES - INDIVIDUAL DATA
119
NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE
-PFHS/PFOS
'
MORTALITY DATA MALES GROUP 1 (VEHICLE CONTROL)
SCHEDULED ANIMAL NECROPSY
OTHER
TREATMENT
FROM
TO
1 03-N0V-98 2 03-N0V-98
3 03-N0V-98 4 03-NOV-98
5 04-N0V-98
6 04-N0V-98
7 04-N0V-98
8 04-N0V-98
9 18-N0V-98 10 18-N0V-98
11 18-N0V-98 13 02-DEC-98 14 02-DEC-98 15 02-DEC-98
06-0CT-98 06-0CT-98 06-0CT-98
06-0CT-98 06-0CT-98
06-OCT-98 O6-0CT-98 06-0CT-98 06-OCT-98 06-0CT-98 06-0CT-98
06-0CT-98 06-0CT-98 06-0CT-98
02-N0V-98 0 2 -NOV-98
02-NV-98 02-N0V-98 0 3 -NOV-98
03-N0V-98 03-N0V-98 03-N0V-98 03-N0V-98 03-N0V-98 03-N0V-98 03-NOV-98 03-N0V-98
03-N0V-98
MALES GROUP 2 (TELOMER ALCOHOL)
SCHEDULED ANIMAL NECROPSY
OTHER
TREATMENT
FROM
TO
17 03-N0V-98 18 03-NOV-98
19 03-N0V-98 20 03-N0V-98 21 04-N0V-98
22 04-N0V-98 23 04-N0V-98 24 04-N0V-98 25 18-N0V-98 26 18-N0V-98 27 18-N0V-98 29 02-DEC-98 30 02-DEC-98 31 02-DEC-98
06-0CT-98 06-0CT-98
06-0CT-98 06-OCT-98 06-0CT-98 06-0CT-98 06-OCT-98
06-0CT-98 06-0CT-98 06-OCT-98 06-0CT-98 06-0CT-98 06-0CT-98 06-0CT-98
02-N0V-9B 02-N0V-98 02-N0V-98 02-N0V-98 03-N0V-98 03-N0V-98 0 3 -NOV-98 03-N0V-98 0 3 -NOV-98 03-N0V-98
O3-N0V-98 03-N0V-98 03-N0V-98 03 -NOV-98
.
BACK TO MAIN
MORT-IND - 1 24-FEB-99
'
1 20
NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/
-PFHS/PFOS
"
MORTALITY DATA
MALES
.
GROUP 3 (TELOMER ACRYLATE)
SCHEDULED ANIMAL NECROPSY
OTHER
TREATMENT
FROM
TO
33 03-NOV-98 34 03-NOV-98 35 03-NOV-98 36 03-NOV-98 37 04-NOV-98 33 04-N0V-98 39 04-N0V-98 40 04-NOV-98 41 18-N0V-98 42 18-N0V-98 43 18-N0V-98 45 02-DEC-98
46 02-DEC-98 47 02-DEC-98
06-OCT-98 06-OCT-98 06-OCT-98 06-OCT-98 06-OCT-98 06-0CT-98 06-OCT-98 06-OCT-98 06-OCT-98 06-OCT-98 06-OCT-98 06-OCT-98 06-OCT-98 06-OCT-98
02-NOV-98 02-N0V-98 02-NOV-98 02-NOV-98 03-N0V-98 03-NOV-98 03-N0V-98 03-NOV-98 03-NOV-98 03-NOV-98 03-N0V-98 03-N0V-98 03-NOV-98 0 3 -NOV-98
.
MALES GROUP 4
SCHEDULED ANIMAL NECROPSY
49 03-N0V-98 50 03-NOV-98 51 03-N0V-98 52 03-NOV-98 53 04-N0V-98 54 04-N0V-98 55 04-N0V-98 56 04-N0V-98 57 18-N0V-98 58 18-NOV-98 59 18-N0V-98 61 02-DEC-98 62 02-DEC-98 63 02-DEC-98
OTHER
TREATMENT
FROM
TO
06-OCT-98 06-OCT-98 06-OCT-98 06-0CT-98
06-OCT-98 06-OCT-98 06-0CT-98 06-0CT-98 06-OCT-98 06-OCT-98 06-OCT-98 06-OCT-98 06-OCT-98 06-OCT-98
02-NOV-98 02-NOV-98 02-N0V-98 02-N0V-98 03-NOV-98 03-NOV-98 03-N0V-98 03-NOV-98 O3-N0V-98 03-NOV-98
0 3 -NOV-98 03-NOV-98 03-NOV-98 03-N0V-98
BACK TO MAIN
MORT-IND - 2 2 4 -FEB-99
.
.
121
NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/
-_ PFHS/PFOS
MORTALITY DATA MALES GROUP 5 (PFHS)
SCHEDULED ANIMAL NECROPSY
OTHER
65 03-NOV-98 66 03-NOV-98 67 03-N0V-98 68 03-NOV-98 69 04-NOV-98 70 04-NOV-98 71 04-N0V-98
72 04-NOV-98 73 18-NOV-98 74 18-N0V-98 75 18-NOV-98
77 02-DEC-98 78 02-DEC-98
79 02-DEC-98
TREATMENT
FROM
TO
06-0CT-98 06-0CT-98 06-0CT-98 06-OCT-98 06-OCT-98 06-OCT-98 06-0CT-98 06-0CT-98 06-0CT-98 06-OCT-98 06-0CT-98 06-OCT-98 06-OCT-98 06-OCT-98
02-NOV-98 02-NOV-98 02-NOV-98 02-NOV-98 03-NOV-98
03-NOV-98 . 03-N0V-98 03-NOV-98 03-NOV-98 03-NOV-98 03-N0V-98 03-NOV-98 03-NOV-98 03-NOV-98
MALES GROUP 6 (PFOS)
SCHEDULED ANIMAL NECROPSY
81 03-N0V-98 82 03-NOV-98 83 03-N0V-98 84 03-N0V-98 85 04-N0V-98 86 04-NOV-98 87 04-NOV-98 88 04-N0V-98 89 18-NOV-98. 90 18-NOV-98 91 18-N0V-98 93 02-DEC-98 94 02 -DEC-98 95 02-DEC-98
OTHER
TREATMENT
FROM
TO
06-0CT-98 06-OCT-98 06-OCT-98 06-0CT-98 06-OCT-98 06-OCT-98 06-0CT-98 06-0CT-98 06-OCT-98 06-0CT-98 06-0CT-98 06-0CT-98 06-OCT-98 06-OCT-98
02-NOV-98 02-NOV-98 02-N0V-98 02-N0V-98 03-NOV-98 03-NOV-98 03-N0V-98 03-N0V-98 03-NOV-98
03-NOV-98 03-NOV-98 03-NOV-98 03-N0V-98 03-NOV-98
BACK TO MAIN
MORT-IND 3 2 4 -FEB-99
.
'
1 22
NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/
PFHS/PFOS
MORTALITY DATA FEMALES GROUP 1 (VEHICLE CONTROL)
.
SCHEDULED ANIMAL NECROPSY
OTHER
TREATMENT
FROM
TO
97 05-NOV-98 98 05-NOV-98 99 05-NOV-98 100 05-N0V-98 101 06-NOV-98 102 06-NOV-98 103 06-NOV-98 104 06-NOV-98 105 20-NOV-98 106 20-NOV-98 107 20-NOV-9B
109 04-DEC-98 110 04-DEC-98 111 04-DEC-98
08-OCT-98 08-OCT-98 08-0CT-98 08-OCT-98 08-0CT-98 08-0CT-98
08-0CT-98 08-OCT-98 08-OCT-98 08-OCT-98 08-OCT-98 08-0CT-98 08-0CT-98 08-OCT-98
04-N0V-98 04-NOV-98 04-NOV-98 0 4 -NOV-98 05-N0V-98 05-NOV-98 05-NOV-98 05-NOV-98 05-NOV-98 0 5 -NOV-98 05-NOV-98 05-NOV-98 05-NOV-98 0 5 -NOV-98
FEMALES GROUP 2 (TELOMER ALCOHOL)
SCHEDULED ANIMAL NECROPSY
OTHER
TREATMENT
FROM
TO
113 05-NOV-98 114 05-NOV-98
115 05-N0V-98
116 05-NOV-98
117 06-NOV-98
118 06-NOV-98
119 06-NOV-98 120 06-NOV-98 121 20-NOV-98 122 20-N0V-98 123 20-N0V-98 125 04-DEC-98 126 04-DEC-98 127 04-DEC-98
08-OCT-98 08-0CT-98 08-0CT-98 08-0CT-98 08-OCT-98
08-0CT-98 08-OCT-98 08-OCT-98 08-OCT-98 08-0CT-98 08-OCT-98 08-0CT-98 08-0CT-98 08-OCT-98
04-NOV-98 0 4 -NOV-98
04-NOV-98 04-NOV-98
05-NOV-98 05-NOV-98 05-NOV-98 05-N0V-98 05-NOV-98 05-NOV-98 05-NOV-98 05-NOV-98 05-NOV-98 05-NOV-98
BACK TO MAIN
MORT-IND - 4 24-FEB-99
1 23
NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/
-,, /PFHS/PFOS
MORTALITY DATA FEMALES GROUP 3 (TELOMER ACRYLATE)
SCHEDULED ANIMAL NECROPSY
OTHER
TREATMENT
FROM
TO
129 130 05-NOV-98 131 05-NOV-98 132 05-NOV-98 133 06-NOV-98
134 06-NOV-98
135 06-NOV-98 136 06-NOV-98 137 20-NOV-98
138 20-N0V-98 139 20-N0V-98 141 04-DEC-98 142 04-DEC-98 143 04-DEC-98
05-N0V-98
08-0CT-98 08-OCT-98 08-OCT-98 08-OCT-98 08-0CT-98 08-OCT-98
08-0CT-98 08-0CT-98 08-OCT-98 08-0CT-98 08-0CT-98 08-OCT-98 08-OCT-98 08-OCT-98
04-N0V-98 04-NOV-98 0 4 -NOV-98 04-N0V-98 0 5 -NOV-98 05-NOV-98
0 5 -NOV-98 05-N0V-98 05-N0V-98 05-NOV-98 05-N0V-98 05-NOV-98 05-N0V-98 05 -NOV-98
FEMALES GROUP 4
SCHEDULED ANIMAL NECROPSY
OTHER
TREATMENT
FROM
TO
145 05-NOV-98
146 05-N0V-98 147 05-NOV-98
148 05-N0V-98
149 06-NOV-98 150 06-N0V-98
151 06-NOV-98 152 06-N0V-98 153 20-NOV-98 154 20-NOV-98 155 20-NOV-98 157 04-DEC-98 158 04-DEC-98 159 04-DEC-98
08-0CT-98 08-OCT-98 08-OCT-98 08-OCT-98 08-0CT-98 08-OCT-98 08-OCT-98 08-0CT-98 08-0CT-98 08-OCT-98 08-0CT-98 08-OCT-98 08-0CT-98 08-0CT-98
04-NOV-98 04-NOV-98 04-NOV-98 04-N0V-98 05-NOV-98 0 5 -NOV-98 05-N0V-98 0 5 -NOV-98 05-N0V-98 0 5 -NOV-98 05-NOV-98 05-NOV-98 0 5 -NOV-98 05-NOV-98
BACK TO MAIN
MORT-IND - 5 24-FEB-99
1 24
NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/
-PFHS/PFOS
'
MORTALITY DATA FEMALES GROUP 5 (PFHS)
SCHEDULED ANIMAL NECROPSY
OTHER
161 05-NOV-98 162 05-NOV-98 163 05-NOV-98 164 05-NOV-98 165 06-N0V-98
166 06-NOV-98 167 06-NOV-98 168 06-NOV-98 169 20-N0V-98 170 20-NOV-98 171 20-NOV-98 173 04-DEC-98 174 04-DEC-98
175 04-DEC-98
FEMALES GROUP 6 (PFOS)
SCHEDULED ANIMAL NECROPSY
OTHER
177 05-N0V-98 178 05-NOV-98
179 05-N0V-98 180 05-NOV-98
181 06-NOV-98
182 06-NOV-98 183 06-NOV-98 184 06-NOV-98 185 20-NOV-98 186 20-N0V-98 187 20-NOV-98 189 04-DEC-98 190 04-DEC-98
191 04-DEC-98
TREATMENT FROM
08-0CT-98 08-OCT-98 08-OCT-98 08-OCT-98 08-OCT-98 08-0CT-98 08-0CT-98 08-0CT-98
08-OCT-98 08-OCT-98 08-0CT-98 08-OCT-98 0 8 -OCT-98 08-0CT-98
04-N0V-98 04-NOV-98 04-NOV-98 04-N0V-98 05-NOV-98
05-N0V-98 05-NOV-98 05-N0V-98
05-NOV-98 05-NOV-98 05-NOV-98 0 5 -NOV-98 05-NOV-98
05-NOV-98
TREATMENT
FROM
TO
08-OCT-98 08-OCT-98
08-OCT-98 08-0CT-98 08-OCT-98 08-0CT-98 08-0CT-98 08-OCT-98 08-OCT-98 08-OCT-98 08-0CT-98 08-OCT-98 08-OCT-98 08-OCT-98
04-N0V-98 04-NOV-98 04-N0V-98 04-NOV-98 05-NOV-98 0 5 -NOV-98 05-NOV-98 05-NOV-98
05-NOV-98 05-NOV-98 05-NOV-98
05-NOV-98 0 5 -NOV-98
05-NOV-98
-
BACK TO MAIN
MORT-IND - 6 24-FEB-99
'
1 25
BACK TO MAIN
NOTOX Project 242933
SYM-IND - 1 25-FEB-99
CLINICAL SIGNS
MALES GROUP 1 (VEHICLE CONTROL)
..
SIGN (MAX.GRADE)
TREATMENT
'
WEEKS: 1 ............ 2 .............3 ............ 4 ............ 5.
DAYS: 1.2.3.4.5.6.7.1.2.3.4.5.6.7.1.2.3.4.5.6.7.1.2.3.4.5.6.7.1.
TIME: ABABABABABABABABABABABABABABABABABABABABABABABABABABABABAB
ANIMAL 2
SKIN / FOR / PLUMAGE ALOPECIA (3) (BACK) ALOPECIA (3) (NECK) ALOPECIA (3)
(FORELEG LEFT) ALOPECIA (3) (HINDLEG LEFT) ALOPECIA (3) (HINDLEG RIGHT)
G: .............. : ........................... 111111111... G: .......................... 1111111111111111111 G: ...1222222222221111111111111111111111111...... G: ..... 11111111111113311111111111111............ G: .... 1111111111111111111111111111111111......
ANIMAL 3
SKIN / FOR / PLUMAGE ALOPECIA (3) (CHEST) SCABS (3) (NECK)
G: .11. G: .
.111111111111111111111111111.
ANIMAL 4
.
SKIN / FUR / PLUMAGE ALOPECIA (3) (BACK)
ALOPECIA (3) (NECK)
G: G:
.111111 1111111
ANIMAL 9
SKIN / FUR / PLUMAGE ALOPECIA (3) (ABDOMEN) ALOPECIA (3) (CHEST)
.
G:
G:
111111111111111111111111111111111111..
ANIMAL 15
VARIOUS
BULGING EYE (1)
(EYE RIGHT)
DARK (3)
'
(EYE RIGHT)
G: G:
NO FURTHER CLINICAL SIGNS NOTED IN THIS GROUP
G: Highest grades Time,after treatment (H.Mi) A: 0.00
B: 4.00
1 26
NOTOX Project 242933
CLINICAL SIGNS, DAILY MALES GROUP 1 (VEHICLE CONTROL)
SIGN (MAX.GRADE)' (LOCATION)
RECOVERY WEEKS: 1.... 2.... 3.....4.... 5.
ANIMAL 9
SKIN / FDR / PLUMAGE
ALOPECIA (3) (CHEST)
. G ...... 12222222.
ANIMAL 15
.
VARIOUS
BULGING EVE (1) (EYE RIGHT) DARK (3) (EYE RIGHT)
G: 11....... G: 11.......
NO FURTHER CLINICAL SIGNS NOTED IN THIS GROUP
BACK TO MAIN
SYM-IND - 2.
25-MAR-99
G: Highest daily grades
1 27
BACK TO MAIN
NOTOX Project 242933
SYM-IND - 3 25-FEB-99
CLINICAL SIGNS
MALES GROUP 2 (TELOMER ALCOHOL)
*.
SIGN (MAX.GRADE)
.
TREATMENT i,,,.........2
.....
......
3............4....
......
,5.
DAYS:' l!2!3!4!5.'6!7!l!2!3!4!5.6!7.l!2.3.4.5.6.7.1.2.3.4.5.6.7.1.
TIME: AHABABABABABABABABABABABABABABABABABABABABABABABABABABABAB
ANIMAL 17
SK IN / FOR / PLMAGE ALOPECIA (3) (NECK)
ANIMAL 1 8
::::::::::::::::::::::::::: G : .............................: ........................................ ; ..............................1 1 1 1 1 ..............
: : : : : ..................... : : : : : : : : : : : : : : : :
SK IN / FOR / PLUMAGE SCABS (3)
(BACK)
ANIMAL 1 9
G : ......................................................................................................................1 ............ : : : : : : : : : : : : : : : : : ..................... : : : :
SK IN / FUR / PLUMAGE ALOPECIA (3) (SMOULDER LEFT)
ANIMAL 20
:::::::::::::::::::::::::: G : ...............................................................................................1 1 ................................
: : : : : : : : : : : : : : : : : : .......... : :
SK IN / FUR / PLUMAGE WOUND (3 ) (GENITAL REGION)
: : : : : : : : : : : : : : ...............................: : : : : G : ......................................................................................................................1 ............
ANIMAL 2 1
: : ....................... : : : : : : : : : : : : : : : : : : :
S K IN / FUR / PLUMAGE SCABS (3)
(NECK)
:::::::::::::::::::::::::: G : ....................................................................................................1 1 .............. 1 1 1 1 .
ANIMAL 23
: : : : : : : : : : ....................... : : : : : : : : : : :
SK IN / FUR / PLUMAGE ALOPECIA (3) (FORELEG LEFT)
:::::::::::::::::::::::::: G : ................................................................................................................ 1 1 1 1 1 1 1 .
ANIMAL 25
SKIN / FUR / PLUMAGE RED STAINING (3) (PERIORBITAL REGION LEFT)
ANIMAL 26
G:
SKIN / FUR / PLUMAGE SCABS (3) (SNOUT)
ANIMAL 27
SKIN / FUR / PLUMAGE RED STAINING (3) (PERIORBITAL REGION RIGHT)
G: G:
1
ANIMAL 29
:::::::::::::::::::::::::::::
BREATHING RALES (3)
SKIN / FUR / PLUMAGE ALOPECIA (3)
(ABDOMEN)
::::::::::::::::::::::::::::: G: ............................................... Ill
G: .......... 1111111111111111111111111111111111111111111111
G: Highest grades T i m e ,after treatment (H.Mi) A: 0.00
B: 4.00
1 28
BACK TO MAIN
NOTOX Project 242933
SYM-IND 25-FEB-99
CLINICAL SIGNS MALES GROUP 2 (TELQMER ALCOHOL)
.
SIGN (MAX.GRADE)
TREATMENT
WEEKS: 1 ............ 2 ............ 3 .............4 .............5. DAYS: 1.2.3.4.5.6.7.1.2.3.4.5.6 .7.1.2.3.4.5.6 .7.1.2.3.4.5.6 .7.1. TIME: ABABABABABABABABABABABABABABABABABABABABABABABABABABABABAB
ANIMAL 30
SKIN / FUR / PLUMAGE ' ALOPECIA (3)
(CHEEK RIGHT)
G:
NO FURTHER CLINICAL SIGNS NOTED IN THIS GROUP
111111111
G: Highest grades Tine after treatment (H.Mi) A: 0.00
B: 4.00
NOTOX Project 242933
CLINICAL SIGNS, DAILY .
MALES GROUP 2 (TELOMER ALCOHOL)
SIGN (MAX.GRADE) (LOCATION)
RECOVERY WEEKS: 1 ..... 2 ..... 3 ..... 4 ..... 5.
ANIMAL 25
SKIN / FUR / PLUMAGE RED STAINING (3) (PERIORBITAL REGION LEFT)
G: ...... Ill....
ANIMAL 26
SKIN / FUR / PLUMAGE SCABS (3) (SNOUT)
G: 11............
ANIMAL 27
SKIN / FUR / PLUMAGE
RED STAINING (3) (PERIORBITAL REGION RIGHT)
G: ... 1 .......
ANIMAL 29
BREATHING RALES (3)
SKIN / FUR / PLUMAGE ALOPECIA (3) (ABDOMEN)
G: 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 , G: 1 1 1 1 1 .......... .
NO FURTHER CLINICAL SIGNS NOTED IN THIS GROUP
BACK TO MAIN
SYM-IND - S 25-MAR-99
G: Highest daily grades
1 30
BACK TO MAIN
NOTOX Project 242933
SYM-IND - 6 2 5 -FEB-99
CLINICAL SIGNS
MALES GROUP 3 (TELOMER ACRYLATE)
SIGN (MAX.GRADE)
TREATMENT
WEEKS: 1 ............ 2 .............3 ............ 4 .............5. nAYS: 1.2.3.4.5.6 .7.1.2.3.4.5.6.7.1.2.3.4.5.6.7.1.2.3.4.5.6.7.1. TIME: ABABABABABABABABABABABABABABABABABABABABABABABABABABABABAB
ANIMAL 33
SKIN / FOR / PLUMAGE ALOPECIA (3) (NECK) ALOPECIA (3)
(CHEST) ALOPECIA (3) (FORELEG RIGHT)
G: iiiiii!.!.!
G: 111222222211111111111111111111111111 *
G: 11122222221111111111...............
ANIMAL 35
SKIN / FOR / PLUMAGE ALOPECIA (3) (FLANK LEFT)
ALOPECIA (3) (CHEST) ALOPECIA (3) (FORELEG RIGHT) SCABS (3) (TAIL)
G: 11112221111111111111 G: ................... G : ..111111111111111111 G: ...................
mm
inumimi..
ANIMAL 45
: : : : : : : : : : : : : : : : : : ..........
SKIN / FOR / PLUMAGE
SCABS (3) (BACK) RED STAINING (3) (NECK)
: : : : : : : : : : : : : : : : i I ::: i i i G: ................... .................. 11...1111
.......... .......................................
NO FURTHER CLINICAL SIGNS NOTED IN THIS GROUP
G: Highest grades Time'after treatment (H.Mi) A: 0.00
B: 4.00
NOTOX Project 242933
CLINICAL SIGNS, DAILY MALES GROUP 3 (TELOMER ACRYLATE)
SIGN (Max.GRADE) (LOCATION)
RECOVERY WEEKS: 1 ..... 2 ..... 3 ..... 4 ..... 5.
ANIMAL 45
SKIN / FUR / PLUMAGE RED STAINING (3) (NECK)
G: .................. 111111
NO FURTHER CLINICAL SIGNS NOTED IN THIS GROUP
BACK TO MAIN SYM-IND - 7
25-MAR-9 9
G: Highest daily grades
1 32
BACK TO MAIN
NOTOX Project 242933 .
S Y M - I N D - ff 2 5 - F E B - 99
CLINICAL SIGNS MALES GROUP 4
SIGN (MAX.GRADE)
.
TREATMENT
-
WEEKS: 1 ............ 2 ............ 3............ 4 ............ 5.
DAYS: 1.2.3.4.5.6 .7.1.2.3.4.5.6.7.1.2.3.4.5.6.7.1.2.3.4.5.6.7.1.
TIME: ABABABABABABABABABABABABABABABABABABABABABABABABABABABABA3
ANIMAL 51
SKIN / FOR / PLUMAGE ALOPECIA (3) (FORELEG RIGHT)
G:
ANIMAL 56
:::::::::::::
SKIN / FUR / PLUMAGE RED STAINING (3) (PERIORBITAL REGION LEFT)
: : : : ........... . : : G: .....................11111
ANIMAL 61
SKIN / FUR / PLUMAGE ALOPECIA (3) (EYE RIGHT)
VARIOUS EYE RIGHT, INJURED (1) DEHYDRATED (3) (EYE RIGHT) BULGING EYE (1) (EYE RIGHT) DARK (3) (EYE RIGHT)
G:
G: G: G: G:
ANIMAL 62
VARIOUS DARK (3)
(EYE RIGHT)
...............................................
ANIMAL 63
: : : : : : : : ........ : : : : : : : : :
SKIN / FUR / PLUMAGE SCABS (3) (NECK)
: : : : : : : : i : : : : i i i ': * : : : i G: ................................ 111111111111
NO FURTHER CLINICAL SIGNS NOTED IN THIS GROUP
11..
G: Highest grades Time .after treatment (K.Mi) A: 0.00
B: 4.00
1 33
NOTOX Project 242933
CLINICAL SIGNS, DAILY .
MALES
'
GROUP 4
SIGN {MAX.GRADE) (LOCATION)
RECOVERY WEEKS: 1 ..... 2 ..... 3 ..... 4 ..... 5.
ANIMAL 61
SKIN / FOR / PLUMAGE
ALOPECIA (3)
(EYE RIGHT) VARIOUS
- EYE RIGHT. INJURED (1) DEHYDRATED (3) (EYE RIGHT) BULGING EYE (1) (EYE RIGHT) DARK (3)
(EYE RIGHT)
G: ........... llllllllllllli.il.
g : liiiiiiiiiiiiiiiimumm.
G: .22222...................... G: 111111...................... G: 111111......................
ANIMAL 62
VARIOUS DARK (3) (EYE RIGHT)
G: 11
NO FURTHER CLINICAL SIGNS NOTED IN THIS GROUP
BACK TO MAIN
SYM-IND - 9 25-MAR-99
G: Highest daily grades
1 34
BACK TO MAIN
NOTOX Project 242933
SYM-IND -lo 25-FEB-99
CLINICAL SIGNS KALES GROUP 5 (PFHS)
STOW (MAX.GRADE)
treatm en t
WEEKS: 1 ............ 2 ............ 3 ...... ......4 .............5. DAYS: 1.2.3.4.5.6.7.1.2.3.4.5.6 .7.1.2.3.4.5.6 .7.1.2.3.4.5.6.7.1. TIME: ABABABABABABABABABABABABABABABABABABABABABABABABABABABABAB
ANIMAL 65
SKIN / FDR / PLUMAGE RED STAINING (3) (EAR LEFT)
G: 11111111111
ANIMAL 67
SKIN / FUR / PLUMAGE RED STAINING (3) (SNOOT)
G:
ANIMAL 72
SKIM / FDR / PLDMAGE
WOUND (3) (BACK)
G
ANIMAL 74
POSTURE HUNCHED POSTURE (1) .
G:
ANIMAL 79
SKIN / FUR / PLUMAGE RED STAINING (3) (NECK) RED STAINING (3) (CERVICAL REGION)
VARIOUS
DARK (3) (EYE RIGHT)
G: G:
G:
NO FURTHER CLINICAL SIGNS NOTED IN THIS GROUP
111
1111
G: Highest grades Time After treatment (H.Mi) A: 0.00
B: 4.00
1 35
NOTOX Proiact 242933
CLINICAL SIGNS, DAILY MALES GROUP 5 (PFHS)
S IG N (MAX.GRADE)
(LOCATION)
RECOVERY
WEEKS: 1 ..... 2 ..... 3 ..... 4 ..... 5.
ANIMAL 74
POSTURE HUNCHED POSTURE (1)
G: 11...
ANIMAL 79
SKIN / FUR / PLUMAGE RED STAINING (3) (NECK) RED STAINING (3) (CERVICAL REGION)
VARIOUS DARK (3)
(EYE RIGHT)
G: .... , . . 1 1 .................. G: .... ....1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 .
G: 11...
NO FURTHER CLINICAL SIGNS NOTED IN THIS GROUP
BACK TO MAIN
SYM -IN D - 1 1
25-MAR-99
G: Highest daily grades
1 36
BACK TO MAIN
NOTOX Project 242933
SYM-IND -12 25-FEB-99
CLINICAL SIGNS HALES ' GROUP 6 (PFOS)
SIGN (MAX.GRADE)
.
TREATMENT WEEKS: 1 ............ 2 ............ 3 ............ 4 .............5. DAYS: 1.2.3.4,5.6.7.1.2.3.4.5.6 .7.1.2.3.4.5.6.7.1.2.3.4.5.6.7.1. TIME; ABABABABABABABABABABABABABABABABABABABABABABABABABABABABAB
ANIMAL 81
SKIN / FUR / PLUMAGE
SWELLING (3)
(ABDOMEN)
.
G:
ANIMAL 87
SKIN / FUR / PLUMAGE ALOPECIA (3) (CHEEK LEFT)
:::::::::::::::::::: G: .......................... 11111111111111
ANIMAL 93
SKIN / FUR / PLUMAGE SCABS (3) (NECK)
!:i! G: ................................. 1111111
ANIMAL 95
::::::
SKIN / FUR / PLUMAGE RED STAINING (3) (HEAD)
:::::: G: ...........
NO FURTHER CLINICAL SIGNS NOTED IN THIS GROUP
mm..
G: Highest grades Time .after treatment (H.Mi) A: 0.00
B: 4.00
NOTOX Project 242933
CLINICAL SIGNS, DAILY MALES GROUP 6 (PFOS)
SIGN (MRX.GRADE) (LOCATION)
RECOVERY WEEKS: 1 ..... 2 ..... 3 ..... 4 ..... 5.
ANIMAL 95
SKIN / FOR / PLUMAGE
RED STAINING (3)
(HEAD)
G : .............................................. 1 1 1 1 1 1 1 1 1
NO FURTHER CLINICAL SIGNS NOTED IN THIS GROUP
BACK TO MAIN
SYM-IND -13 2 5 - M A R - 99
G: Highest daily grades
1 38
BACK TO MAIN
NOTOX Project 242933
SYM-IND - M25-FEB-99
CLINICAL SIGNS
FEMALES GROUP 1 (VEHICLE CONTROL)
.
S IG N (MAX.GRADE)
TREATMENT
wp'Rtf.Q 1 .# 2................. 3 .........................
4 . .
5
D A Y S :' l ! 2 ! 3 . ' 4 ! 5 .' 6.' 7 ! l ! 2 ! 3 ! 4 ! 5 ! 6 ! 7 ! l ! 2 . ' 3 ! 4 ! 5 ! 6 ! 7 ! l ! 2 ! 3 ! 4 . ' 5 !6 .' 7 ! l . '
TIM E: ABABABABABABABABABABABABABABABABABABABABABABABABABABABABAB
ANIMAL 97
VARIOUS MISSING (1) (TAIL APEX)
ANIMAL 98
VARIOUS MISSING (1) (TAIL APEX)
ANIMAL 104
SKIN / FUR / PLUMAGE ALOPECIA (3) (BACK)
ANIMAL 105
SKIN / FUR I PLUMAGE SCABS (3) (ABDOMEN) WOUND (3)
(ABDOMEN)
ANIMAL 106
SKIN / FUR / PLUMAGE ALOPECIA (3) (NECK)
ANIMAL 109
SKIN / FUR / PLUMAGE ALOPECIA (3) (FLANK RIGHT) ALOPECIA (3) (THIGH HIND LEFT) ALOPECIA (3) (THIGH HIND. RIGHT)
ANIMAL 110
SKIN / FUR / PLUMAGE ALOPECIA (3) (ABDOMEN)
SECRETION / EXCRETION SALIVATION (3)
ANIMAL 111
SKIN / FUR / PLUMAGE ALOPECIA (3) (GENERAL) ALOPECIA (3) (CHEEK LEFT) ALOPECIA (3) (BACK) ALOPECIA (3) (ABDOMEN) ALOPECIA (3) (FLANK LEFT) ALOPECIA (3) (FLANK RIGHT)
G: 1 U U 1 U 1 1 I U U 1 1 1 1 1 U I U 1 1 U U ..
G: u u i i m n u u i i i i i u u u i u u ..
:::::::::::::::::::::::: : : : .............. G: ...............................111111111......
: : : : : : : : : : : : : : ............ : : : : : : : : G: ................................................... 1111111
G: .................................................. 1 ......
.............. G:
:
********* G:
G: . . . 1 1 1 1 1 1 1 1 1 1 1 ................. .
G: 1 1 1 1 1 1 1 1 1 1 1 1 1 1 ................. .1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 .... Ill
G: ........... 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 G:
* G:
**************
G:
G:
G: 1 1 1 1 2 2 2 2 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 ......
G:
G:
* ** ******** 1111111111111111111
G: Highest grades Time^after treatment (H.Mi) A: 0.00
B: 4.00
1 39
BACK TO MAIN
NOTOX Project 242933
SYM -IN D - IS 25-F E B -9 9
CLINICAL SIGNS
FEMALES . GROUP 1 (VEHICLE CONTROL)
.
SIG N (MAX.GRADE)
ALOPECIA (3) (SHOULDER LEFT) ALOPECIA (3) (HINDLEG LEFT)
TREATMENT WEEKS: 1 ............................... 2 ................................3 ............................... 4 ........................... 5 DAYS: 1 . 2 . 3 . 4 . 5 . 6 . 7 . 1 . 2 . 3 . 4 . 5 . 6 . 7 . 1 . 2 . 3 . 4 . 5 . 6 . 7 . 1 . 2 . 3 . 4 . 5 . 6 . 7 . 1 . TIM E :' ABABABABABABABABABABABABABABABABABABABABABABABABABABABABAB
G: ........................................................................1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 .................
G : ......................................................................................... 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 .............. ..
NO FURTHER C L IN IC A L SIGN S NOTED IN T H IS GROUP
G: Highest grades Time after treatment (H.Mi) A: 0.00
B: 4.00
NOTOX Project 242933
CLINICAL SIGNS, DAILY
FEMALES
GROUP 1 (VEHICLE CONTROL)
SIGN (MAX.GRADE) (LOCATION)
RECOVERY WEEKS: 1 ..... 2 ..... 3 ..... 4 ..... 5.
ANIMAL 105
SKIN / FUR / PLUMAGE SCABS (3)
(ABDOMEN)
. G: ...1111111111..
ANIMAL 106
SKIN / FUR /. PLUMAGE ALOPECIA (3) (NECK)
G: ......... 1111.
ANIMAL 109
SKIN / FUR / PLUMAGE ALOPECIA (3) (FLANK RIGHT) ' ALOPECIA (3) (THIGH HIND LEFT) ALOPECIA (3) (THIGH HIND RIGHT)
G: ....... 1111... G: ...111111111111 G: 111111111111111
ANIMAL 111
SKIN / FUR / PLUMAGE
ALOPECIA (3) (GENERAL) ALOPECIA (3) (CHEEK LEFT) ALOPECIA (3) (BACK) ALOPECIA (3) (ABDOMEN) ALOPECIA (3) (FLANK LEFT) ALOPECIA (3) (FLANK RIGHT)
G: ................ 11111111111
G ............
11..........
G: ..... 11111111111..........
G ........11111111111..........
G: 11111111111111111
G: 11122222222222222
NO FURTHER CLINICAL SIC2JS NOTED IN THIS GROUP
BACK TO MAIN
SYM-IND -l6 25-MAR-99
G: Highest daily grades
BACK TO MAIN
NOTOX Project 242933
SYM-IND - 17 25-FEB-99
CLINICAL SIGNS
FEMALES
.
GROUP 2 (TELOMER ALCOHOL)
.,
SIGN (MAX.GRADE)
TREATMENT
WEEKS: 1 ............ 2 .............3 ............ 4 .......... ..5. DAYS: 1.2.3.4.5.6 .7.1.2.3.4.5.6.7.1.2.3.4.5.6.7.1.2.3.4.5.6.7.1. TIME: ABARABABABABABABABABABARABABABABABABABABABABABABABABABABAB
ANIMAD 113
SKIN / FUR / PLUMAGE ALOPECIA (3) (FORELEGS)
G:
ANIMAL 117
SKIN / FUR / PLUMAGE RED STAINING (3) (EAR RIGHT)
G:
.11.
ANIMAL 119
SKIN / FUR / PLUMAGE ALOPECIA (3) (BACK)
G:
ANIMAL 121
SKIN / FUR / PLUMAGE ALOPECIA (3) (HEAD) ALOPECIA (3) (CHEEK LEFT) ALOPECIA (3) (CHEEK RIGHT) ALOPECIA (3)
(NECK) SCABS (3) (FLANK LEFT)
G: G: G: G: G:
ANIMAL 122
SKIN / FUR / PLUMAGE ALOPECIA (3) (CHEEK LEFT) ALOPECIA (3)
(CHEEK RIGHT) SCALES (3) (CHEEK LEFT) SCALES (3) (CHEEK RIGHT)
G: G: G: G:
ANIMAL 123
SKIN / FUR / PLUMAGE ALOPECIA (3) (CHEEK LEFT)
G:
ANIMAL 126
SKIN / FUR / PLUMAGE ALOPECIA (3) (NECK)
G: 11111111111111111.
NO FURTH ER CLINICAL SIGNS NOTED IN THIS GROUP
.1111111112.. .11111 .11
. 11.
.1111111111.
G: Highest grades Time,after treatment (H.Mi) A: 0.00
B: 4.00
1 42
NOTOX Project 242933
~"
CLINICAL SIGNS, DAILY FEMALES GROUP 2 (TELOMER ALCOHOL)
SIGN (MAX.GRADE) (LOCATION)
RECOVERY WEEKS:
ANIMAL 121
.
SKIN / FUR / PLUMAGE
ALOPECIA (3) (HEAD)
ALOPECIA (3) (CHEEK LEFT) ALOPECIA' (3) (CHEEK RIGHT)
G: ...... 2222211 G .1112222222222. G ___ 1222222222.
ANIMAL 122
SKIN / FUR / PLUMAGE ALOPECIA (3) (CHEEK LEFT) ALOPECIA (3) (CHEEK RIGHT) SCALES (3) (CHEEK LEFT) SCALES (3) (CHEEK RIGHT)
G .... 112222222. G ___ 1222222222. G ...... 1 1 1 1 1 1 1 . G ...... 1 1 1 1 1 1 1 .
NO FURTHER CLINICAL SIGNS NOTED IN THIS GROUP
BACK TO MAIN
SYM-IND -IS 2 5 - M A R -9 9
G: Highest daily grades
1 43
BACK TO MAIN
NOTOX Project 242933
SYM-IND - 1g 25-FEB-99
CLINICAL SIGNS
FEMALES GROUP 3 (TELQMER ACRYLATE)
.
SIGN (MAX.GRADE)
treatment
WEEKS: 1 .......... ..2.............3 ............ 4 ............ 5. DAYS: 1.2.3.4.5.6.7.1.2.3.4.5.6.7.1.2.3.4.5.6.7.1.2.3.4.5.6.7.1. TIM E: ABABABABABABABABABABABABABABABABABABABABABABABABABABABABAB
ANIMAL 129
POSTURE HUNCHED POSTURE (1)
ANIMAL 131
SKIN / FUR / PLUMAGE ALOPECIA (3) (CHEST) RED STAINING (3) (HEAD) RED STAINING (3) (NECK)
ANIMAL 132
SKIN / FUR / PLUMAGE ALOPECIA (3) (ABDOMEN) ALOPECIA (3) (SHOULDER LEFT) RED STAINING (3) (HEAD) RED STAINING (3) (BACK) RED STAINING (3) (NECK)
ANIMAL 133
POSTURE
HUNCHED POSTURE (1)
ANIMAL 134
SKIN / FUR / PLUMAGE SCABS (3) (NECK)
ANIMAL 136 .
SKIN / FUR / PLUMAGE SCABS (3) (BACK) RED STAINING (3) (HEAD)
ANIMAL 137
SKIN / FUR / PLUMAGE ALOPECIA (3) (ABDOMEN)
ANIMAL 138
POSTURE . HUNCHED POSTURE (1)
SKIN / FUR / PLUMAGE PILOERECTION (1) ALOPECIA (3) (ABDOMEN)
:::::::::::::::::: G: ............ ;............. 111111111
...... G: ___ G: ___
::
G: ___ G: ___ G: .... G: ___ G: ....
* * 1 * * ..........
..1 1 1 1 1 1 1 ......... ....... m i ....
...
.......
........................................................ ....
G: ........... .ililililili.!. !
:::::::::::::::::::::::::::::
::::::::::::::::::::::::::::: G: .................................................... 111111
G: G:
::::::::::: G: 1111222211111.111....
.11 1
G:
G: ............................................. G: ...................................... 1111111
iiiiiiii
G: Highest grades Tine After treatment (H.Mi) A: 0.00
B: 4.00
1 44
BACK TO MAIN
NOTOX Project 242933
S Y M-IND - 20 25-FEB-99
CLINICAL SIGNS FEMALES GROUP 3 (TELOMER ACRYLATE)
..
SIGN (MAX.GRADE)
TREATMENT
WEEKS: 1 ............ 2 ............ 3 ............ 4 ............ 5. DAYS: 1.2.3.4.5.6 .7.1.2.3.4.5.6 . 7.1.2.3.4.5.6 .7.1.2.3.4.5.6 .7.1. TIME: ABABABABABABABABAHABABABABABABABABABAEABABABABABABABABABAB
ANIMAL 139
.
POSTURE HUNCHED POSTURE (1)
SKIN / FUR / PLUMAGE ALOPECIA (3) (ABDOMEN)
G: G:
............................................. 1 1111111111111122222122222222222222222211111111
ANIMAL 141
::::::::::::::::::
SKIN 1 FDR / PLUMAGE RED STAINING (3) (HEAD)
: .................. : : : : : : : : G: ...................................
ANIMAL 142
: : : : : : : : : ................
SKIN / FUR / PLUMAGE RED STAINING (3) (HEAD)
G: .................... .....111......
ANIMAL 143
: : : : : : .......... : : : : : :
SKIN / FUR / PLUMAGE ALOPECIA (3)
(ABDCMEN) FISSURES WITH BLEEDINGS (3) (PAW HIND RIGHT) RED STAINING (3) (PAW HIND RIGHT)
:::::::::::::::::::::::::::: : G: 1111222211111111.1111111.111111111111111111111111111111111 G: .......................................11................. G: .........................................111111111111111..
NO FURTHER CLINICAL SIGNS NOTED IN THIS GROUP
G: Highest grades Time.after treatment (H.Mi) A: 0.00
B: 4.00
1 45
NOTOX Project 242933
CLINICAL SIGNS, DAILY FEMALES GROUP 3 (TELOMER ACRYLATE)
SIGN (MAX.GRADE) (LOCATION)
RECOVERY WEEKS: 1...;..2..... 3 ..... 4 ..... 5.
ANIMAL 138
POSTURE
h u n c h e d POSTURE (1)
SKIN / FUR / PLUMAGE
PILOERECTIQN (1)
G: 11111111111111. G: .1111......
ANIMAL 139
POSTURE HUNCHED POSTURE (1)
SKIN / FUR / PLUMAGE ALOPECIA (3) (ABDOMEN)
G: 1111111....... G: 11111111111111.
ANIMAL 141
SKIN / FUR / PLUMAGE RED STAINING (3) (HEAD)
G: ................ .........
ANIMAL 142
SKIN / FUR / PLUMAGE RED STAINING (3) (HEAD)
G: ..........................
NO FURTHER CLINICAL SIGNS NOTED IN THIS GROUP
BACK TO MAIN
SYM-IND -21 2 5 - M A R - 99
G: Highest daily grades
1 46
BACK TO MAIN
NOTOX Project 242933
S Y M - I N D - 22. 25-FEB-99
CLINICAL SIGNS
FEMALES
GROUP 4 (
)
SIGN (MAX.GRADE)
TREATMENT
.
WEEKS: 1 ............ 2 .............3 ............ 4 .............5.
DAYS: 1.2.3.4.5.6 .7.1.2.3.4.5.6 .7.1.2.3.4.5.6 .7.1.2.3.4.5.6.7.1.
TIME: a b a b a b a b a b a b a b a b a b a b a b a b a b a b a b a b a b a h a b a b a b a b a b a b a b a b a b a b a b
ANIMAL 147
SKIN / FUR / PLUMAGE ALOPECIA (3) (BACK)
G:
111111111111. . .1111111111111____ 1111111
ANIMAL 148
SKIN / FUR / PLUMAGE SHELLING (3) (EAR RIGHT) RED STAINING (3) (EAR LEFT) RED STAINING (3) (EAR RIGHT)
G: G: G:
.1111111111111... .......... 111111111......... ......... .11111111111111111111111111111111111111
ANIMAL 151
SKIN / FUR / PLUMAGE ALOPECIA (3) (NECK)
G:
.11111111
ANIMAL 152
POSTURE HUNCHED POSTURE (1)
SKIN / FUR / PLUMAGE RED STAINING (3) (EAR RIGHT)
G; G:
. . .1111111111111111111111111111 111111111111111111111111111111111111
ANIMAL 155
.
SKIN / FUR / PLUMAGE RED STAINING (3) (EAR RIGHT)
G: ................................................ 1111111111
ANIMAL 157
SKIN / FUR / PLUMAGE
ALOPECIA (3)
(NECK)
ALOPECIA (3)
(THROAT REGION)
SCALES (3)
(THROAT REGION) '
G: G: G:
.222222222222222222222222222222221111 . 1111111111111.
ANIMAL 159
SKIN / FUR / PLUMAGE
ALOPECIA (3)
G:
(NECK)
SCABS (3)
G:
(NECK)
RED STAINING (3)
G:
(EYE RIGHT)
RED STAINING (3)
G:
(PERIORBITAL REGION RIGHT)
SECRETION / EXCRETION
WATERY DISCHARGE FROM EY (3) G:
(EYE RIGHT)
.111111111111111111111111111111111111
NO FURTHER CLINICAL SIGNS NOTED IN THIS GROUP
G: Highest grades
Time -after treatment (H.Mi) A: 0.00
B: 4.00
1 47
N O T O X Pro-iect 242933
CLINICAL SIGNS, DAILY
FEMALES GROUP 4 ( )
SIGN (MAX.GRADE) (LOCATION)
RECOVERY WEEKS: 1 ..... 2 ..... 3 ..... 4 ..... 5.
ANIMAL 159
SKIN / FUR / PLUMAGE
SCABS (3)
G: .......
(NECK)
RED STAINING (3)
G: ...11...
(EYE RIGHT)
RED STAINING (3)
G: .... 11.
(PERIORBITAL REGION RIGHT)
SECRETION / EXCRETION
WATERY DISCHARGE FROM EY (3) ............ .
(EYE RIGHT)
.11
NO FURTHER CLINICAL SIGNS NOTED IN THIS GROUP
BACK TO MAIN
S Y M - I N D - 2.3 2S-MAR-99
G: Highest daily grades
1 48
BACK TO MAIN
NOTOX Project 242933
SYM-IND - 54 25-FEB-99
CLINICAL SIGNS FEMALES GROUP 5 (PFHS)
SIGN (MAX.GRADE)
TREATMENT
WEEKS: 1 ............ 2 ............ 3 .............4 ............ 5. DAYS: 1.2.3.4.5.6 .7.1.2.3.4.5.6.7.1.2.3.4.5.6.7.1.2.3.4.5.6 .7.1. TIME: a b a b a b a b a b a b a b a b a b a b a b a b a b a b a b a b a b a b a b a b a b a b a b a b a b a b a b a b a b
ANIMAL 162
SKIN / FUR / PLUMAGE ALOPECIA (3) (NECK) SCABS (3) (NECK) WOUND (3) (NECK)
G:
G: 1111112222111111111111222222111111111111111111111111111...
G:
ANIMAL 163
POSTURE HUNCHED POSTURE (1)
G:
ANIMAL 166
SKIN / FUR / PLUMAGE
ALOPECIA (3) (NECK)
G: ..................... 1111111111.
ANIMAL 167
SKIN / FUR / PLUMAGE ALOPECIA (3) (NECK)
"
G:
.111111111111111.
ANIMAL 169
SKIN / FUR / PLUMAGE ALOPECIA (3) (BACK)
SECRETION / EXCRETION SALIVATION (3)
G: G:
ANIMAL 173
SKIN / FUR / PLUMAGE
ALOPECIA (3)
(NECK)
ALOPECIA (3) (ABDOMEN)
ALOPECIA (3)
'
(SHOULDER RIGHT)
G: G: G:
.11111111111111111111132.
...... i m m u n i . ..
ANIMAL 174
SKIN / FUR / PLUMAGE - ALOPECIA (3)
(BACK) ALOPECIA (3) (ABDOMEN)
G: G:
....... 11111112111111.
.1111111111222222222222222222222222111111111111
ANIMAL 175
SKIN / FUR / PLUMAGE ALOPECIA (3) (NECK) ALOPECIA (3) (HINDLEG RIGHT)
G: G:
.illuminili.
NO FURTHER CLINICAL SIGNS NOTED IN THIS GROUP
G: Highest grades Time'after treatment (H.Mi) A: 0.00
B: 4.00
1 49
NOTOX Project 242933
CLINICAL SIGNS, DAILY FEMALES GROUP 5 (PFHS)
S IG N (MAX.GRADE)
(LOCATION)
RECOVERY
WEEKS: 1 ..... 2 ..... 3 ..... 4 ..... 5 .
ANIMAL 173
SKIN / FUR / PLUMAGE ALOPECIA (3) (NECK)
G: .... 11111111111111111111111
ANIMAL 174
SKIN / FUR / PLUMAGE ALOPECIA (3)
(BACK)
ALOPECIA (3)
(ABDOMEN)
. G: ........................ u
G: 111----111111111111111111111
ANIMAL 175
SKIN / FUR / PLUMAGE
ALOPECIA (3) (NECK) ALOPECIA (3)
(HINDLEG RIGHT)
G: 111...... 111111111111111111
G: ..... 1111........
...........
NO FURTHER CLINICAL SIGNS NOTED IN THIS GROUP
BACK TO MAIN
SYM-IND - 25 2 5 - M A R - 99
G: Highest daily grades
BACK TO MAIN
NOTOX Project 242933
--
SYM-IND 2 5 -- F E B * 99
CLINICAL SIGNS FEMALES GROUP 6 (PFOS)
SIGN (MAX.GRADE)
.
TREATMENT WEEKS : 1 ............ 2 .............3 ............ 4 ............ 5. DAYS: 1 .2 . 3 . 4. 5 . 6.7 .1 . 2 . 3 .4 .5 . 6.7 . 1.2 .3 . 4. 5. 6. 7 . 1.2. 3 . 4 . 5. 6. 7 .1 . TIME! ABABABABABABABABABABABABABABABABABABABABABABABABABABABABAB
ANIMAL 178
POSTURE
HUNCHED POSTURE (1) SKIN / FUR / PLUMAGE
RED STAINING (3)
(SNOUT)
G G
111111.............
............... 1111...
ANIMAL 179
POSTURE HUNCHED POSTURE (1)
SKIN / FUR / PLUMAGE ALOPECIA (3) (FORELEG LEFT) ALOPECIA (3) (FORELEG RIGHT)
G G G
..... 11111111..............
111111112. . 222222222222222..
222222222. . 222222222222222. .
ANIMAL 183
'
SKIN / FUR / PLUMAGE SCABS (3) (NECK)
G:
1111111
ANIMAL 185
SKIN / FUR / PLUMAGE RED STAINING (3) (HEAD)
G:
ANIMAL 186
SKIN / FUR / PLUMAGE ALOPECIA (3) (ABDOMEN)
RED STAINING (3) (HEAD)
G: G:
liiiiiiiiiiiiiiiiiiiiiii.!. !
ANIMAL 187
SKIN / FUR / PLUMAGE RED STAINING (3) (HEAD) RED STAINING (3) (EAR LEFT)
RED STAINING (3) (EAR RIGHT) FED STAINING (3) (NECK)
ANIMAL 189
SKIN / FUR / PLUMAGE ALOPECIA (3) (ABDOMEN)
. . . . . . . . .............................. G: ............... 1111......................................
ANIMAL 190
........... .. : : ............. : ...........: : : : : :
SKIN / FUR / PLUMAGE ALOPECIA (3)
(ABDOMEN)
:::::::::: i : ! ! ! !i !i ::::::: i : : G: .........................111111..... 111111... 111111111111
NO FURTHER CLINICAL SIGNS NOTED IN THIS GROUP
G: Highest grades Time After treatment (H.Mi) A: 0.00
B: 4.00
NOTOX Project 242933
CLINICAL SIGNS, DAILY FEMALES GROUP 6 (PFOS)
SIGN (MAX.GRACE) (LOCATION)
RECOVERY
'
WEEKS: 1 ..... 2 ..... 3,.... 4 ..... 5.
ANIMAL 185
SKIN / FUR / PLUMAGE RED STAINING (3) (HEAD)
G: . . . 1 1 .........
ANIMAL 186
SKIN / FUR / PLUMAGE RED STAINING (3) (HEAD)
G: ...1 1 1 1 1 1 1 1 1 1 1 .
ANIMAL 187
SKIN / FUR / PLUMAGE RED STAINING (3) (HEAD) RED STAINING (3) (EAR LEFT) RED STAINING (3) (EAR RIGHT)
RED STAINING (3) (NECK)
G: 1113211.... 1 1 . G: ...1 1 1 1 1 1 1 1 1 ... G; ........ 1 1 1 ... G:
ANIMAL 190
SKIN / FUR / PLUMAGE ALOPECIA (3) (ABDOMEN)
G: 1 1 1 1 1 ..........
NO FURTHER CLINICAL SIGNS NOTED IN THIS GROUP
BACK TO MAIN
SYM-IND - 27 2 5 -MAR-99
G: Highest daily grades
1 52
BACK TO MAIN
NOTOX Project 242933
BODY WEIGHTS (GRAM) MALES
~~
BW-IND - 1
1 7 -M A R -9 9
TREATMENT
DAYS MEEKS
ANIMAL
1 1
5 1
GROUP 1 (VEHICLE CONTROL)
1 232 219 2 202 253 3 228 252 4 196 214 5 209 235 6 221 249 7 222 245 8 197 221
9 207 236 10 184 206
11 207 232 12 -- -- -- 13 208 238 14 190 217 15 211 233 16
GROUP 2 (TELCMER ALCOHOL)
17 224 245 18 230 261 19 219 241 20 209 235 21 189 217 22 228 254 23 216 241 24 228 248 25 205 230 26 190 209
27 197 223 28 ------ ...... 29 208 232 30 217 249 31 212 234 32
GROUP 3 (TELQMER ACRYLATE)
33 220 243 34 191 216 35 224 242 36 211 233 37 220 243 38 211 236 39 206 231 40 206 232 41 216 245 42 197 220
43 210 235 44 -- -- ---
45 201 227
46 213 242
47 206 229
48 --
--
GROUP 4
49 222 239
50 201 228 51 217 246 52 211 244 S3 209 236
8 12 15 2 23
235 257 274 297 274 ' "300 230 246 258 278 270 288 269 297 244 266 247 274 207 229
240 264
----
250 2S1 227 254 243 270
262 311 313 255 288 304 316 280 277 226
277 . --
285 262 275
265 286 299 280 307 329 259 277 288 251 270 285 238 270 287 277 304 321 262 288 302 264 284 300 237 265 265 217 237 245
235 256 260
-- -- ----
----
240 270 277 257 290 297 245 267 273
261 278 296 234 259 273 250 267 277 250 269 281 263 278 290 261 282 298 251 280 287 255 276 290 261 292 294 227 257 262
246 275 288 ----
235 269 271
249 278 283
234 267 270
-" --
--
248 268 278 244 268 280 264 294 310 267 294 308 252 278 288
1 53
19 22
25
3 44
281 289 301 332 341 354 341 351 360 271 282 288 301 308 322 319 334 350 341 349 368 297 304 317 303 310 322 245 251 260
295 310 316
---- --
311 323 345 286 299 309 309 319 328
319 329 354 359 311 322 300 307 .310 ' 322 343 348 322 328 307 321 289 292 269 277
283 288 -- '.
292 298 320 333 289 297
342 384 327 320 338 363 336 333 308 287
3_03
310 347 313
317 327 346 288 301 312 294 306 314 301 313 323 304 316 327 320 327 340 314 322 334 306 319 327 319 331 355 289 300 310 302 313 326 _ -- w ---- 304 310 326 304 311 325 298 313 324
------
294 303 308 301 303 316 336 345 . 360 335 338 347 315 324 346
28 4
309 365 377 298 330 356 381 322 336 276
328 -- 356 322 342
359 389 342 328 348 386 343 346 319 294
311 ---- 317 360 320
352 318 329 328 331 355 349 332 365 325
337 ---- 341 336 338 --
324 333 378 360 354
BACK TO MAIN
NOTOX Project 242933
BODY WEIGHTS (GRAM) MALES
BW-IND - 2 17-MAR-99
RECOVERY
DAYS
WEEKS ANIMAL
1
1 ..
5 1
8 12 14 19 22 25 28 2223444
GROUP 1 (VEHICLE CONTROL)
1 2 ---- 3 4 5 6
7 8 9 318 348 10 253 279
11 310 339 12 13 334 370 14 297 337 15 314 354 16 -- --
GROUP 2 (TELOMER ALCOHOL)
17 18 -- 19 -- 20 21 22 23 24 25 298 330 26 277 303
27 289 318
28
29 289 315
30 336 385
31 296 334
32 -- --
-- -----
GROUP 3 (TELOMER ACRYLATE)
33 -- --
.34
-- --
--
35 -- -- - --
36 -- ---- '
37 -- --
38 -- --
39 -- --
40
----
----
41 336 388
42 298 314
43 312 347 44 -- -- -- -- -- 45 314 352 46 312 351 47 310 351 48
SOUP 4 r
49
--.
--
50 -- --
51 -- --
52 -- --
53 -- - --
--
359 285 351 381 341 361
---
--
337 308
330
317 388 345
--
----
-- -- --
-- --
--
----
390 325
.
350
361 357 354
--
--- -- --
---
--
364 299 362 394 348 379
--
--
346 319 342 334 402 357
--
-- --- -- ---- -- -- --- --
404 341 359
--..
379 375 373
-- -- -- -- --
--
373 303 366 398 353 378
---
--
354 320 345 328 401 358
--
-- -- -- --- -- -- ----
408 347 359
...
. 378 379 372
... -- -- -- --
--
--
--
--
414 365 393
--
--
--
--
-- 342 413 371
--
--- -- ---- -- -- -- ... ... --
... ...
395 391 381
____ ... -- -- --
--
-- -- --
421 373 403
. --------
--
--
--- --
435 385 415
---
--
-- -- --
440 387 419
--------
------
--
--
--
349 416 381
--
. --------
--
--
--
353 430 389
--
. ---------
--
355 429 388
--
-- -- -- -- ---- --- ____ ... --
... ...
403 402 393
-- -- -- -- -- -- ... -- ... --
--...
412 411 398
----
-- -- -- --- -- -- ... ... --
-- ----
414 413 409
... --. -- -- ...
... ... -- -- --
-- ... -- -- ...
BACK TO MAIN
NOTOX Project 242933
BODY WEIGHTS (GRM) MLES
BW-IND - 3 17-MAR-99
TREATMENT
DAYS WEEKS ANIMAL
1 5 8 12 15 19 22 25 28 112233444
GROUP 4 1
54 55 56 57 58 59 60 ' 61 62 63
223 206 200 206 203 199
229 215 205
64 _____
GROUP 5 (PFHS)
65 234 66 199 67 211 68 214 69 204 70 226 71 208 72 211 73 222 74 . 189
75 197 76 .--77 206 78 184 79 200 80
GROUP 6 (PFOS)
81 198 82 203 83 200
84 213 85 222 86 213 87 227 88 211 89 222 90 212
91 204 92 -- --
93 187 94 220 95 211 96 --
252 270 296 232 246 263 223 232 249 240 252 ' 290 235 247 270 228 237 266
264 278 317 238 253 283 230 240 268
---- --
257 217 232 233 225 257 234 . 237 254 203
220 ---- 233 210 226
282 231 248 249 246 281 258 259 268 201
231 --- 240 211 238
310 254 270 273 269 305 282 280 297 224
264
--
267 233 255
300 268 252 298 281 276
327 288 274
---
284 267 281 283 273 318 296 291 307 219
266 -- 272 236 255
221 242 263 283
231 2 52 2 7 5 283
228
248 '
269
281
240 260 281 295
254 277 314 328
237 252 275 279
256 281 310 327
236 252 279 292
253 270 304 . 317
240 254 284 289
2-- -3-4
242 ----
276 ------
282
204 207 229 . 227 247 250 277 276 237 246 275 275
---- -- --
321 330 290 293 267 266 336 348 309 321 299 304
359 369 316 321 291 300
_____
___ __
351 364 282 285 300 304 301 310 296 295 343 355 315 317 310 314 338 343 241 245
296 307 --. 299 306 263 ' 271 276 285
300 310
298 302
300 307 311 314 360 371 294 295 350 361 312 320 343 352 321 335
318
______
333 .
242 247 299 305 295 301
----
340 352 306 313 278 285 374 398 334 348 314 325 380 397 345 359 316 329
___
381 392 298 299 315 327 322 324 308 . 315 375 379 330 337 329 334 365 380 255 256 321 333 ___ 318 327 287 290 291 301
322 331
312 327
316 325 333 338 388 394 311 322 380 381 336 345 374 386 349 364 351 362
-- 261 265 320 325 312 321
-- ---
1 55
BACK TO MAIN
NOTOX Project 242933
BODY WEIGHTS (GRAM) MALES
BW-IND - 4 1 7 - M A R - 99
RECOVERY
DAYS
WEEKS ANIMAL
1 1
GROUP 4 (
54 55 -- 56 -- 57 365 58 321 59 301 60 -- 61 362 62 326 63 302
64 --
GROUP 5 (PFHS)
65 66 -67 -- 68 -- 69 -- 70 -- 71 -- 72 73 352 74 234
75 307 76 ------ 77 305 78 267 79 279 80
GROUP 6 (PFOS)
81 82 83
84 85
86 87 88 89 90
----
-- -- -- -- ... 354 340
91 339 92 -- 93 245 94 306 95 293 96
5 8 12 14 19 22 25 28
12
22344 4
--
--
--
--
---- -- -- --
-- -- ... -- ------ ... . ...
414 426 449 456 --
363 367 383 383 --
338 344 357 356 --
-- -- -- -- ----
-----
... ... ... ... ... ... ---- -- ... ... ...
406 424 437 429 453 462 473 468 370 383 402 398 420 428 441 443 342 353 370 368 383 387 396 394
------ -------- --
-- --- -- --- --- -- . -- ---
---- ----
-- --
-*-- -- ----
-- --
-- --
-- --
-- -- -- -- -- -- -- ...
--
--- -- -
--
--
--
.-- --
-- -- -- -- -- -- -- --.
--- -- -
. -- - -- .. ... ___ ...
397 412 420 418 --
... ... __
264 274 277 275 --
--
--
--
342 350 365 361 ... ... ... ...
---
----
--- ------
--
... ... ...
345 356 357 360 369 373 392 386
301 314 321 324 335 339 360 350
313 ---
319 325 ---
331 --
337 --
341 --
353 --
350 --
--
-- --
--
--
--
--
--
----
--
--
-- . -- . .--
--
-- -- -- -- --- -- - -- --
-- -- -- -- -- .-- -- --
-- --- -- -- -- - -- . -- --
---- --- --
-- --
-- .--
---- ... __
-- .--
. -..-.-
408 412 428 429 __ _--
... --
380 384 397 404 -- -- -- ' --
377 390 405 408 .--
... -- . --
-- -- -- ------ -- -- ... ... ... ...
276 284 296 298 306 315 331 326
343 349 362 362 381 387 396 401
329 338 356 355 . 366 375 385 384
1 56
BACK TO MAIN
N O T O X Project: 242933
BODY WEIGHTS (GRAM) FEMALES
BW-IND - 5 1 7 -MAR--9 9
TREATMENT
DAYS MEEKS ANIMAL
1 1
'
5 1
GROUP 1 (VEHICLE CONTROL)
97 168 172 98 164 170 99 165 171 100 179 178 101 182 190 102 194 200 103 163 164 104 179 193 105 158 169 106 196 210
107 108 109 . 110 111 112
172
163 169 184
180
--
.162 180 192
GROUP 2 (TELCMER ALCOHOL)
113 170 176 114 182 191 115 182 184 116 180 188 117 157 167 118 168 174 119 163 168 120 166 182 121 163 170 122 163 171
123 175 183 124 ------ 125 162 165 126 164 168 127 166 182 128
GROUP 3 (TELCMER ACRYLATE)
129 170 179 130 154 161 131 171 171 132 175 180 133 153 164 134 172 181 135 181 192 136 166 170 137 157 164 138 167 177
139 158 157 140 -- -- 141 154 163 142 160 164 143 183 192 144
GROUP "4 ( )
145 167 176 146 181 193 147 181 187 148 152 154 149 184 185
8 12 . 15 19 22
2 2 33
4
176 189 200 210 217 179 193 196 209 207 180 195 199 213 212 193 208 197 218 220 208 221 234 246 248 204 212 231 238 243 172 180 177 189 200 201 213 222 235 233 171 189 189 200 202 218 237 241 255 257
185 202 200 213 206
~ - ----
------
...
166 179 181 188 195 185 204 200 219 215 197 211 216 230 235
184 206 195 194 173 176 178 191 168 169
187
------
172 166 184
191 220 203 207 184 190 193 198 178 184
200
---
184 181 205
205 213 230 249 208 222 218 229 191 204 200 212 202 ' 211 211 218 178 189 180 193
204 216
... ...
186 204 183 197 202 220
222 255 219 233 200 216 213 214 195 206
221
...
203 199 223
185 192 190 205 204 167 177 175 186 191 186 195 201 211 212 193 201 209 212 221 166 176 180 191 192 192 197 198 206 218 198 216 222 237 244 183 192 184 199 204 167 181 188 204 212 176 184 182 . 185 183
162 175 176 191 197
-- -- ------
169 186 1 S6 200 197 166 182 178 191 192 199 215 215 230 229
180 193 195 204 201 197 210 218 233 229 187 205 219 231 237 159 175 183 194 196 203 215 216 230 238
1 57
25 4
222 208 224 228 267 248 207 248 217 268
225
...
199 238 243
233 266 234 242 214 227 220 238 208 212
236
...
209 208 230
220 206 218 228 203 234 250 218 218 195 202
--
203 205 249
217 248 242 207 240
28 4
220 217 227 244 268 251 206 252 219 275
232
...
204 247 243
227 271 238 242 224 221 233 241 208 209
238
...
216 211 236
225 208 222 231 203 236 249 215 214 201
199
--
206 204 248
220 250 243 205 248
BACK TO MAIN
NOTOX Project 242933
BODY WEIGHTS (GRAM) FEMALES
,,
BW-IND - 6 1 7 - M A R - 99
RECOVERY
DAYS WEEKS ANIMAL
1 1
5 1
8 12 14 222
GROUP 1 (VEHICLE CONTROL)
97 -- --
98 99 100
101
-----
-- -- -- . --
102 103
---
-- --
104 --
105 200 223
106 249 277
107 206 225
108 -109 185 2X0 110 217 245 1 1 1 222 251 112 -- ---
GROUP 2 (TELCMER ALCOHOL)
113 114 115 116 117 118 119 120
121 ' 122
--------191 193
-- -- ---- --
*-- ----
211 218
123 214 241 124 -- ------ 125 198 225 126 194 226 127 211 237 128 -- -- GROUP 3 (TELCMER ACRYLATE)
129 -130 -- -- 131 -- -- 132 -- -- 133 -- -- 134 -- ' -135 -- -- 136 -- -- -- 137 201 232 138 177 198
139 182 215 140 -- ------ 141 190 211 142 184 211 143 221 249 144 -- --
GROUP 4 ( )
145 -146 -- -- 147 -- -- 148 -- -- 149 -- --
-- -- -- -- -- --- -- ---- 222 282
237 -- 214 257 254
-- -- -- -- -- -- -- ---- 227 288
243 ---- 219 264 262 ----
-- ---- -- -- -- -- .... 243 301
247 --- 220 267 261 ---
-- --- -- ------
--- --
--
-- --- --
------
-- --- -- ... .--
215 223 217 222 232 223
244 254 255
------ ----
--
222 232 236
227 235 232
246 --
2--52
2--58
--- -- - _ --- -- ---- ------ -- --- -- ------ -- ---- -- -- --- ... 235 247 244 199 212 211 215 229 224 ---- ------ -- 212 220 224 216 225 218 255 267 267 -- --- --
--- -- - -- -- ------ ------ -- --- --
1 58
19 22 25 28. 344 4
-- -- - --. --- -- --- -- ---- -- --
-- -- 222 272 267 --
-- -- --. ---- ----... --. --
_ ---
226 275 274 --
-- -- -- -- -- -- --
-- --
-- . ' --- . 230 281 278 --
-- -- -- -- -- -- -- ... -- --
... ...
237 290 278 --
-- -- -- -- -
------ --
--- --
-- ' ---
--- -- -- --
.-- -- -- --
--- -- --
... ...
__
___
-- ... -- --
------ --
... --
---
... __ --~_ ...
243 247 248 244
234 240 242 244
260 --
265 --
2-6--3
266 ...
-- ---- - -- ---- -- ------ ---- __ -... ___ ... ----
-- ...
. _-- _
232 239
228 229
276 277
----
----- -- -- : -- -- -- ... --
--
...
235 233 278
--
-- .--
--
... --
-- -- -- --
-- ... 232 234 278
-- -- --- -- ------ -- -- -- -- -- . -- -- -- --.
BACK TO MAIN
NOTOX Project 242933
BODY WEIGHTS (GRAM) FEMALES :
BW-IND - 7 1 7 - M A R -9 9
TREATMENT
DAYS
WEEKS ANIMAL
1 1
5 1
8 12 15 19 22 25 28 2 233 44 4
GROUP 4 t )
150 172 181 192 213 222 232 234 237 245
151 160 167 170 187 194 196 209 223 222
152 162 169 178 188 186 202 206 221 225
153 158 159 159 172 177 187 196 205 207
154 177 192 194 214 211 229 232 251 255
155 156
163 --
169
--
173
---
191
--
188
--
199 -- .
2--06-
2_17
219 ___
157 167 173 172 192 195 212 212 213 218
158 162 169 169 189 188 199 196 218 210
159 194 207 210 231 238 251 262 269 272
160
-- . --
--
--
--
--
--
--
--
GROUP 5 (PFHS)
161 166 171 179 191 192 205 208 210 216 162 169 177 185 203 200 211 210 222 227 163 167 172 179 187 182 197 204 214 214 164 171 172 181 191 194 207 206 218 223 165 157 155 167 180 191 202 210 214 220 166 173 172 187 195 203 216 226 237 234 167 166 162 164 180 189 200 202 209 2 11 168 185 190 197 213 224 233 240 250 250 169 177 189 191 204 203 223 221 231 236 170 167 174 177 187 186 200 203 217 215
171 168 183 187 204 199 214 222 232 237
172 -- -- ------
-- -- -- __
___
173 175 177 181 196 194 202 209 220 216 174 175 185 185 202 204 221 223 236 236 175 153 161 164 176 179 193 190 204 206 176 -- -- -- -- -- -- -- -- --
GROUP 6 (PFOS)
177 177 187 197 212 215 229 233 245 240 178 154 152 162 172 169 179 179 185 186 179 173 181 187 193 184 196 202 210 212 180 169 167 174 184 186 192 194 200 194 181 185 182 188 194 198 204 201 205 206 182 169 178 179 190 199 208 207 217 220 183 172 172 184 196 193 202 209 215 210 184 164 166 178 191 184 198 204 206 212 185 161 176 172 189 189 211 209 212 204 186 161 173 172 189 192 202 199 203 .206
187 188
157 --
159 --
168 ---
180 ------
178
187
194 --
201 --
203. ----
189 160 162 165 179 178 187 188 193 197
190 157 163 167 180 179 195 200 208 215
191 166 172 169 181 180 186 183 192 193
192 -- --
---- -- --
----
1 59
B A C K TO MAIN
NOTOX P r o j e c t 242933
BODY W EIGHTS (GRAM)
FEM A LES
:
BW-IND - 8 17-MAR-99
recovery
DAYS WEEKS ANIMAL
1 1
5 8 12 14. 19 22 25 28 12223444
GROUP 4
150 -151 -152 -153 189 154 230 155 201 156 -157 205 158 197 159 252
160 --
GROUP 5 (PFHS)
161 -162 -163 -164 -165 -166 -167 -168 -169 220 170 198
171 216 172 -173 200 174 216 175 188 176 --
GROUP 6 (PFOS)
177 --178 -179 -180 -181 -182 --183 -184 -185 188 186 187
187 184 188 -189 178 190 196 191 177 192 . --
----
----
---- ---- 221 228 257 263 228 234 ---- -- 225 233 220 214 267 268
----
...
--
--
-- ' --
--
--
--
236 220
243 -- 226 249 211 --
--
--
-- --
--
--
-- 247 227
245 ---- 225 251 217 --
-- -- -- --. '---
--
---- 216 212
207
197 216 194 "
--
-- -- -- *
--
-- 218 219
214
204 222 201 --
------ -- --~ -- -- ----. 238 236 -- . 273 271 -- 238 234 ---
----
237 235 232 222 237 240 280 277 286
------
... ___ ___
---- -- --
---
--
----
-- --
-- --
------
--- --
---
--
--
._
248 248 __ 236 232 --
258 257 ...
---- --
...
233 226 238
256 254 258
227 --
229 --
234 --
-- -- -- --- -- -
--
--- 226 221
223 ------ 209 227 209
-- --
--. --. -- -- -- 222 220
223 -- .. 215 228 213 ----
-- -- ------
--
__ __ --
... ... 219 239 220 ...
-- -- ... . . . ... -- ... ... '__
... ... ___
... ___ ... -- ----- --
... ... ...
240 250 241 245 293 . 301
243 240 303
----
--
... ... __
-- ---
----
--
... ---
--
-- . . . . ---
-- .-- --
---- --
... . . .
...
... ... ...
---- --
... ... ...
... ... ...
239 250 249 263 273 269
237 --
239 --
244 --
---- --
-- -- . .--
--.
--.
---
---- --
-- ... ...
---- --
... ... ...
... __
...
---- --
__ ... ... __ __ ___
218 219 221
235 238 244
218 220 222
... --
...
1 60
BACK TO MAIN
NOTOX Project 242933
BODY WEIGHT GAIN (%) NALES
BWG-IND - 1 17-MAR-99
TREATMENT
DAYS WEEKS
ANIMAL
1 1
5 1
GROUP 1 (VEHICLE CONTROL)
1 0 -6
2 0 25 3 0 11 4 09 5 0 12 6 0 13 7 0 10 8 0 12
9 0 14 10 0 12
11 12
------0 --12
13 0 14
14 0 14
15 16
--0 --10
GROUP 2 (TELCMER ALCOHOL)
17 0 9 18 0 13 19 0 10 20 0 12 21 0 15 22 0 11
23 0 12 24 0 9 25 0 12 26 0 10
27 0 13 28 --- ------ 29 0 12 30 0 15 31 0 10 32
GROUP 3 (TELCMER ACRYLATE)
33 0 10 34 0 13 35 0 8 36 0 10 37 0 10 38 0 12 39 0 12 40 0 13 41 0 13 42 0 12
43 0 12
44
--
...
45 0 13
46 O' 14
47 0 11
48 --- --
GROUP 4
49 . 0 50 0 51 ' 0 52 0 53 0
8 13 13 16 13
8 12 22
15 19 22 25 28
33
4
44
.
1 36 20 17 23 22 21 24 19 13
----16*
20 19
--15--
11 47 32 26 33 30 34 35 32 24
--28--
35 34
- --2--8
13 54 37 30 38 38 42 42 34 23
----34--
37 38
--30
21 25 64 69 50 54 38 44 44 47 44 51 54 57 51 54 46 50 33 36
. .. . .5.0
50 55 51 57
--46- --51
30 75 58 47 54 58 66 61 56 41
_. .53.
66 63 55
33 81 65 52 58 61 72 63 62 50
. .5.8
71 69
--62
18 28 33 42 47 53 60 22 33 43 54 56 67 69 18 26 32 42 47 49 56 20 29 36 44 47 53 57 26 43 52 64 70 79 84 21 33 41 50 53 59 69 21 33 40 49 52 56 ' 59 16 25 32 35 41 46 . 52 16 29 29 41 42 50 56 14 25 29 42 46 51 55
19 30 32 44 46 54 58 -- ------ ... ... ... ... ...
15 30 33 40 43 49 52 18 34 37 47 53 60 66 16 26 29 36 40 48 51
19 26 35 23 36 43 12 19 24 18 27 33 20 26 32 24 34 41 22 36 39 24 34 41 21 35 36 15 30 33
17 31 37 --- ------ ...
17 34 35 17 31 33 14 30 31 -- ---- . ...
12 21 21 33 22 35 27 39 21 33
1 61
25 39 43 46
38
44. 51 31 43 38 52 52 49 48 47
__44
51 43 45 --
49 58 37 48 44 55 56 55 , 53 52
49 ...
54 46 52 --.
57 63 40 53 49 61 62 59 64 57
55 ---
62 53 57 --
32 36 39 50 51 57 55 59 66 59 60 64 51 55 66
60 66 47 55 50 68 69 61 69 65
60 ...
70 58 64 ...
46 66 74 71 69
BACK TO MAIN
NOTOX Project 242933
B O D Y W E I G H T G A I N (%) MALES
BWG- IND - 2 17 - M A R - 99
*
RECOVERY
DAYS
1 5 8 12 14 19 22 25 28
WEEKS
1 1 2 2 2 3 4 4 4-
ANIMAL
GROUP 1 (VEHICLE CONTROL)
1 -- -- --
2 3 4
-- -- . -- --- -- -- ---- --
5 6
--- -- - -- ------
7 ------
8 -- .... --
. 9 54 68 73
10 38 52 55
11 50 64 70
12 ---
----
13 61 78 83
14 56 77 79
15 49 68 71
16
GROUP 2 (TELQMER ALCOHOL) -
17
18 19 20 21 22 23 24 25 26
-- ---- -- -- -- -- --
45 46
-- -- -
--- --- -- --
61 59
27 47 61 28 ... ---- 29 39 51 30 55 77 31 40 58 32
GROUP 3 (TELCMER ACRYLATE)
33 -- -- 34 ... -- 35 -- -- . 36 -- 37 -- ... 38 ... , -39 -- --- 40 -- --
41 56 80
42 51 59
-- ------ -- -- -- --*
64 62
68 ------
52 79 63
-- -- --. ... .-- .-- . -- -- ai
65
43 49 65 67 44 .-- -- .--
45 56 75 80 46 46 65 68 47 50 70 72
48
GROUP 4 (
49 50 51 52 53
-- -- -- -- --
--- -- ---- ---- ... -- ----
-- ---- -- -- -- -- --.
76 63
75 ----
89 83 80
-- -- -- -- ---- -- --
69 68
74 ----
61 85 68
-- ... ... -- -- -- -- ...
87 73
71 ...
89 76 81
-- -- .. -- -- --
------ ----
-- -- -- ... --
-- -- --- ... ...
------ -- --
-- ... -- -- --
-- -- -- -- ---
-- -- -- -- ---
-- - ... ... ...
80 -- 65 --
--- -- __ ------
77 -- -- ... ---- -- ... ... ...
91 99 102 109 112 86 92 96 103 104 79 86 91 97 99
----
-- ...
...
-- ---
----
-- -- ... -- ...
------ -- --
-- -- ,-- --- -- ------ -- --
------ -- --
... ... __ -- ...
73 .- ... -- 68 -- -- --
-- ' --
75 -- ----
--- --
--
-- ...
58 64 68 70 71
85 90 92 98 98 69 75 80 83 83
-- --- --- . ---- --- -- ---- -- .--
-- ... ... 89 ... 76 --
71 ... ... .--
88 97 78 84 81 . 85
--. --- ... --- -- -- -- __ ... --
... ... 100
89 91
-- --- ... --
-- . -- -- .-- ... --
-- ...
105 93 93
-- --. -- -- -- . ... ... .-- -- --
-- --
106 94 99
... ... __ ... -- ------ -- --
------ -- --
-- -- -- -- ... ------ -- --
1 62
BACK TO MAIN
NOTOX Project 242933
BODY WEIGHT GAIN (%)
MALES
;
BWG-IND - 3 17-MAR-99
TREATMENT
DAYS WEEKS
ANIMAL
1 5 8 12 15 19 22 25 28 112233444
GROUP 4 ( )
54 0 55 0 56 0 57 0 58 0 59 0 60 61 0 62 0 63 0
64 --
GROUP 5 (PFHS)
65 0 66 0 67 0 68 0 69 0 70 0 71 0 72 0 73 0 74 0
75 0 76 -- 77 0 78 0 79 0 80 --
GROUP 6 (PFOS)
81 0 82 0 83 0 84 0 85 0 86 0 87 0 88 0 89 0 90 0
91 0 92 93 0 94 0 95 0 96
13 . 13
12 17 16 15
-----
15 11 12
-----
21 19 16 22 22 19
------
21 18 . 17
. . --
10 21 9 16
10 18 9 ' 16
10 21 14 24 13 24 12 23 14 21
76
12 17
----- --
13 17 14 15 13 19 -- ~--
12 14 14 13 14 11 13 12 14 13
15
----
9 12 12
22
24
24 22 25 18 24 19 22 20
19
--
11 14 17
33 28 25 41 33 34
----
38 32 31
--
32 28 28 28 32 35 36 33 34 19
34
------
30 27 28
---
33 35 35 32 41 29 37 32 37 34
35
--
22 26 30
35 44 30 41 26 34 45 63 38 52 39 50
----
43 57 34 47 34 42
----
21 50 34 42 33 42 32 41 34 45 41 52 42 51 38 47 38 52 16 28
35 50
----- ----
32 45 28 43 28 38
-- .-----
43 52 39 4 7 41 50 38 46 48 62 31 38 44 54 38 48 43 55 36 51
38
------
56
--
21 29 25 36 30 40
48 52 58 42 49 52
33 39 43
69 82 93
58 65 71
53 58 63
------
------
------
61 66 73 49 60 67 46 54 60
------
56 63 68 43 50 50 44 49 55 45 50 51 45 51 54 57 66 68 52 59 62 49 56 58 55 64 71 30 35 35
56 63
-- -----
69
49 54 59 47 56 58
43 46 51
-- --- 1 ----
57 . 49 54 47 67 38 59 52 59 58
63
----
32 39 43
63 54 58 56 75 46 67 59 68 65
72
------
40 45 48
67 61 63 59 77 51 68 64 74 72
77
--~--
42 48 52
1 63
BACK TO MAIN
NOTOX Project 242933
BODY WEIGHT GAIN (%) MALES
BWG-IND - 4 1 7 - M A R - 99
RECOVERY
DAYS WEEKS
ANIMAL
1 1
5 1
8 12 14 19 22 25 28
22
2
34
44
GROUP 4 i )
54 55
56 57 58 59 60 61 62 63
------ --
---
---
--
--
----
--
77 101 107 118 121
----
58 79 81 89 89 -- -- --
111 111 coH*
51 70 73 79 --- -- -- -- --
79
58 77 85 91 87 98
107 104
52 72 78 87 85 95 99 105 106
47 67 72 80 80 87 89 93 92
64 . . .
GROUP 5 (PFHS)
65 -- -- -- --
66 -- -- -- -- 67 -- --- -- --
68 ' -- -- --
--
69 -- -- -- --
70 -- -- -- --
71 -- -- -- --
72 --
------ ------
73 59 79 86 89 88
74 24 40 45 47 46 -- -- -- --
75 . 56 74 78 85 83
76
---- ------ ----
----
77 48 67 73 73 75 79 81 90 87
78 45 64 71 74 76 82 84 96 90
79 40 57 60 63 66 69 71 77 75
80 -- - - -
---
GROUP S (PFOS)
81
82 83
-- --
--
--
84
85
86
87
88
89 . 59 84 86 93
90 . 60 79 81 87
93 91
91 66 85 91 99 100 92 93 31 48 52 58 59 94 39 56 59 65 65 95 39 56 60 69 68 96 -- - -- -- --
::: --
--
64 68 77 73 76 80 73 78 82
74 82 82
164
BACK TO MAIN
NOTOX Project 242933
BODY WEIGHT GAIN (%) FEMALES
BWG-IND - 5 1 7 - M AR-99
TREATMENT
nAYS WEEKS ANIMAL
1. 1
5 1
8 12 15 19 22 25 2 2334 4
GROUP 1 (VEHICLE CONTROL)
97 ' 98 99 100 101 102
103 104 105 106 .
0 0 0 0
0
0 0
0
0 0
2 4 4 -1 4
3 1
8 7 7
107 0 5
108 109 0 - 1 110 0 7 111 0 4
112
GROUP 2 (TELCMER ALCOHOL)
113 0 4 114 0 5 115 0 1 116 0 4 117 0 6 118 0 4 119 0 3 120 0 10 121 0 4 122 0 5
123 124
_0 _5
125 0 2
126 0 2
127 128
--0 --10
GROUP 3 (TELCMER ACRYLATE)
129 0 5 130 0 5 131 0 0 132 0 3 133 0 7 134 0 5 135 0 6 136 0 2 137 0 4 138 0 6
139 140
_0 ---1
141 0 6
142 0 3
143 144
0 --
--5
GROUP 4 (
145 0 5 146 0 7 147 0 3 148 0 1 149 0 1
' 5 9 9 8
14 5 6
12 8
11
8
2 9 7
8 13
7 8 10 5 9 15 3 4
--7
6 1
--1 1
9 8 9 10 8 12 9 10 6 5
--3
10 4 9
--
8 9 3 5 10
13 19 25 29 32 18 20 27 26 27 18 21 29 28 36 16 10 22 23 27 21 29 35 36 47
9 19 . 23 25 28 10 9 16 23 27 19 24 31 30 39 20 20 27 28 37 21 23 30 31 37 17 16 24 20 31 10 11 15 20 22 21 18 30 27 41 15 17 25 28 32
12 21 25 31 37
21 26 37 40 46
12 14 22 20 29
15 21 27 29 34
17 22 30 27 36
13 19 26 29 35
18 24 29 31 35
19 27 31 29 43
9
9
16
20
28
13 10 18 26 30
--14
17 ---
--23
-
26 --
35 ---
14
15
26
25 .
29
10 12 20 21 27
--23 --22 --33
34
--39--
13 12 21 20 29
15
14
21 .
24
34
14 18 23 24 27
15 19 21 26 30
15 18 25 25 33
15 15 20 27 36
19 23 31 35 38
16 11 . 20 23 31
15 20 30 35 39
10 9 11 10 17
11 --
--1 1
21 --
25 --
28 --
21 21 30 . 28 32
14 11 19 20 28
17 17 26 25 36
-- ------
16 17 22 20 30 16 20 29 27 37 13 . 21 28 31 34 15 20 28 29 36 17 17 25 29 30
1 65
28 4
31 32 38 36 47 29 26 41 39 40
35
25 46 32
34 49 31 34 43 32 43 45 28 28
36 --
33 29 42
32 35 30 32 33 37 38 30 36 20
26 --
34 28 36
32 38 34 35 35
BACK TO MAIN
NOTOX Project 242933
B O D Y W E I G H T G A I N {%) FEMALES
* .
BWG- IND - 6 17 -MAR-99
RECOVERY
CAYS
1 5 8 12 14 19 22 25
WEEKS
1 1 2 2234 4
ANIMAL
GROUP 1 (VEHICLE CONTROL)
97
98 99 100
101 102
-- --- -- -- -- -- -- -- --- -- -- -- -- --- . -- -- --------
103 104
-- -- - -- -- ---- -- -- --- -- --
105 27 41 41 44
106 27 41 44 47
107 20 31 38 41 108 ---- -- --- ------
109 13 29 31 34
110 28 45 52 56
1 1 1 21 36 38 42
112 ---
--
GROUP 2 (TELOMER ALCOHOL)
113 114 115
116 . 117 118 119
--
--
--
-- -- --
-- -- --
-- -- --
---- ---- -- --
---- ---- ----
120 -- -- --
----
121 17 29 32 37
122 18 34 36 42
123 124 125 126 127 128
22 38 39 45
----
-- -- ---
---
22 39 37 43
18 38 38 43
27 --
43 --
48 --
--52
GROUP 3 (TELOMER ACRYLATE)
129 130 131
---- -- --
--- -- -- --
132
--
--
--
--
133 134
---- ----
-- --
-- --
135 -- -- -- --
136 .- -- - -- ---
137 28 48 50 57
138 6 19 19 27
139 15 36 36 45 140 -- -- -- * --
141 23 37 38 43
142 15 32 35 41
143 21 36 39 46
144
"- --
--
---
GROUP 4 < .
145 146 147
-- -- --
-- --
--
----
148 149
-- --
-- . -- ----
-- --
-- --- -- ---- ---- -- ---- --- --
54 -- 54 --
44 . --
--- -- 35 36 58 61 42 45
--- --
---- -- --.. -... ----- '
... --
39 63 49 --
-- -- -- -- -- -- ... -- --
... ---
41 66 51 --
----
-- . --
---- ---- ---- -- -- .- ...
33 -- 37 --
46 -- ... ---
46 50 41 43 --55 --57
---- ---- ---- -- ------ -- - . -_-- -- _ ... ----
..- -- ... ...
52 53 46 48 --60 --5-8
-- -- --
-- -- --
-- -- ---
------
------
-- -- - ---
... ... ...
55 --
_-
26 . --
--
42 ... ... ... -- - _--
45 51 55
36 43 43
46 --
51 -- .
--51
--
-... -- --. ... -- --
-- ...
53 46 52 --
-- -- ----
----
----
--- --
-- --- ---
----
1 66
28 4
-- ... -- -- -- -- ... -- --
... ...
45 72 51 --
-- -- -- -- -- -- ... -- --
-- ...
51 49
. .6.0
-- ... -- ... -- -- --- --
-- ---
51 46 52 ...
...
... ...
--
BACK TO MAIN
NOTOX Project 242933
BODY WEIGHT GAIN {%) FEMALES
-
BWG-IND - 7 17-MAR-99
TREATMENT
DAYS . WEEKS
ANIMAL
1 1
5 1
8 12 15 19 22 25 28
22
33
44
4
GROUP 4 {
150 151 152 153 154 155 ' 156 157
158 159
0 0 0 0 0 0
0 0 0
5 12 24 29 35 36 38 42 4 6 17 21 23 31 39 39 4 10 16 15 25 27 36 39 1 1 9 12 18 24 30 31 8 10 21 19 29 31 42 44 4 6 17 15 22 26 33 34
4
3
15 . 17
27
27
31
31
4 4 17 16 23 21 35 30
7 8 19 23 29 35 39 40
160 -- -- --
--
--
--
______
----- --
--
GROUP 5 (PEHS)
161
03
8 15 16 .23 25 27 30
162
05
9 20 18 25 24 31 34
163
03
7 12
9 18 22 28 28
164
01
6 12 13 21 20 27 30
165
0 -1
6 15 22 29 34 36 40
166
0 -1
8 13 17 25 31 37 35
167
0 -2
-1
8 14 20 22 26 27
168
03
6 15 21 26 30 35 35
169 0 7 8 15 15 26 25 31 33
170
04
6 12 11 20 22 30 29
171 0 9 11 21 18 27 32 38 41
172
173
01
3 12 11 15 19 26 23
174 0 6 6 15 17 26 27 35 35
175 0 5 7 15 17 26 24 33 35
176 --
---
--
--
--- --
--
--
--
GROUP 6 (PFOS)
177 0 6 11 20 21 29 32 38 36
178
0 -1
5 12 10 16 16 20 21
179
05
8 12
6 13 17 21 23
180 0 - 1 3 9 10 14 15 18 15
181 0 -2 2 5 7 10 9 1 1 11
182
05
6 12 18 23 22 28 30
183 0 0 7 14 12 17 22 25 22
184
01
9 16 12 21 24 26 29
185
09
7 17 17 31 30 32 27
186
07
7 17 19 25 24 26 28
187 xioa oa
01
7 . 15 13 19 24 28 29
189
01
3 12 11 17 18 21 23
190
04
6 15 14 24 27 32 37
191 0 4 2 9 8 12 10 16 16
192
----
--
--- --
--
--
*-- --
1 67
BACK TO MAIN
NOTOX Project 242933
BODY WEIGHT GAIN (%) FEMALES
SWG-IND - 8 17-MAR-99
RECOVERY
DAYS WEEKS ANIMAL
1 1
5 1
8 2
12 2
14 2
19 34
22 4
25 4
28
GROUP 4 ( )
150
151 -- 152 -- 153 20 154 30 155 23 156 -- -- 157 23 158 22 159 30
160 --
GROUP 5 (PEHS)
161
162 --
163
----
164 --
165 -- -
166 --
167 --
168 -- --
169 24
170 19 .
171 29 172 -- -- 173 14 174 23 175 23 176 -- ~
GROUP 6 (PPOS)
177 178 -- 179 --
180 -- 181 -- 182 -- -- 183 -- -- . 184 -- 185 17 186 16
187 17 188 -- -- 189 11 190 25 191 7 192 --
-- ----
40 45 40
----
35 36 38
--
-- -- -- -- -- ---
33 32
45
---
29 42 38 ---
-- -- ---- -- -----
34 32
32
---
23 38 17
----
--.
---- *
44 49 44
--
40 32 38
--
----
---- ...
51 49 54 53 46 44
----
---
42 41 37 46 44 43
-- . .--
-- ---- ----. -- .. -- ...
39 48 47
--
--... ... ... -- ...
44 49 51
. ----
... ... ... ... -- --
50 51 55
--
----'
---- --
-- .
-- ------
40 36
46
--
29 43 42 --
--
-- --
--- --
-- ----
40 41
54
---
33 46 48 ---
--
-- -- -- -- --. ---
40 39
53
---
29 45 50
---
-- -- -- --- -- -- __ . ____ --
... ____
36 47 53
--
-- ... -- -- -- ... ... ... --
... ___
37 50 55
--
-- ... -- -- -- .-- ...
--
... ____
43 56 56
--
-- --
--
-- --
-- --
35 36
36
----
28 41 21
---
-- -- -- -- -- ... --
40 37
42
--
31 45 26
---
-- -- ---- --
38 37
42
...
34 45 28
----
-- -- --- -- ..
... ... ... --
.....
...
37 52 33
...-
.
.-- -- ----
_-- .
... --
... ...
36 50 31
...
-- --
. ----- .-- ... ... ... --
... .i-- -
37 52 33
--
... ... ... ... -- ...
46 48 56
--
-- ... -- ... -- ... ... ... --
... ____
42 54 59
...
---- -- -- ... ____ ... ... --
... ...
38 55 34
---
1 68
BACK TO MAIN
NOTOX Project 242933
~
FOOD CONSUMPTION (G/ANIMAL/DAY) MALES
FC-IND - 1 01 -MAR- 9 9
CAYS. WEEKS CAGE
TREATMENT
1-8 1/2 .
8-15 2/3
15-22 3/4
22-28 4
RECOVERY
1-8 1/2
8-14 2
14-19 2/3
19-22 3/4
GROUP 1 (VEHICLE CONTROL)
1 26 26 27 28 2 27 29 29 31 3 24 25 28 29 4 29 31 34 33
___ __ __ __
___ ___
29 29 _
32 31 30 31
GROUP 2 (TELCMER ALCOHOL)
5 28 29 30 31 6 28 30 29 31 7 24 25 27 28 8 26 29 30 30
__ __
__ __ __ ___
27 27 ___ ___
29 29 28 29
GROUP 3 (TELCMER ACRYLATE)
9 26 27 28 30
_
10 11
28 29 30 30 26 28 31 32
__
__
__
29 30 ___ ____
12 27 28 32 31
32 32 30 32
GROUP 4 ( ) ' 13 27 29 29 30 . 14 27 28 28 29 15 25 29 33 34 16 27 29 32 31
_
_ 33 32 __
_
31 30 30 31
GROUP 5 (PFHS)
17 26 28 28 29 18 29 30 31 32 19 24 26 30 30 20 24 25 29 28 GROUP 6 (PROS) 21 28 29 28 29 22 28 30 30 31 23 27 30 34 36 24 26 27 29 29
-- ----
30 29
_
30 28
----
26
___
27
_ __ __ __
_____
__
____
_____
33 33 --
--
28 27 27 28
1 69
NOTOX Project 242933
FOOD CONSUMPTION (G/ANIMAL/DAY) MALES
DAYS WEEKS CAGE
RECOVERY 22-28
'4
GROUP 1 (VEHXCLE CONTROL)
1 ' -2 3-- 4 31
GROUP 2 (TELOMER ALCOHOL)
5-- 6-- 7 --8 28
GROUP 3 (TELOMER ACRYLATE)
9 10 11 12 30
GROUP 4 (
13 14 15 16 29
GROUP 5 (PFHS)
17 18 19 --
20 27
GROUP 6 (PFOS)
21 22 -- 23 -- 24 27
BACK TO MAIN
FC-IND - 2 0 1 - M A R - 99
1 70
NOTOX Project 242933
FOOD CONSOMPTION (G/ANIMAL/DAY) FEMALES
DAYS WEEKS CAGE
TREATMENT
' 1-8 1/2
8-15 2/3
15-22 3/4
22-28 4 '
GROUP 1 (VEHICLE CONTROL)
25 18 20 21 22 26 20 21 22 23 27 20 21 23 25 28 19 21 24 25
GROUP 2 (TELOMER ALCOHOL)
29
19 .
21
22
24
30 19 21 21 23
31
17 . 20
22
22
32 18 20 23 23
GROUP 3 (TELOMER ACRYLATE)
33 18 19 20 22 34 21 22 22 24 35 18 20 22 21 36 19 21 23 23
GROUP 4
37 19 20 21 23
38 19 21 22 23
39
20 22 23
24
40 19 22 23 23
GROUP 5 (PEHS)
41 19 19 21 22 42 22 23 24 24 43 19 21 23 23 44 19 22 23 23
GROUP 6 (PPOS)
45 22 20 20 20 46 18 19 19 19 47 17 19 19 19 48 19 20 21 20
BACK TO MAIN
FC-IND - 3 0 1 - M A R - 99
RECOVERY
1-8 1/2
8-14 2
14-19 2/3
19-22 3/4
..... ... __ '...
25 24 25 24
... ... ___
22
... _
_. . . .
...
23 22 ...
24 23 22
_
... ... ' ... 23 22 __ 24 23 22
__ _ ... ...
25 24 ... 23 22 20
... ...
__ . . .
...
25 24 -_
24 23 20
_ _ __ _- _ __ 21 20 __
21 21 19
... ... ...
24
... ...
23
... ...
22
... ...
21
... ... ....
22
... -- ...
19
1 71
NOTOX Project 242933
FOOD CONSOMPTION (G/ANIMAL/DAY) FEMALES
RECOVERY
DAYS WEEKS CAGE
22-28 4
GROUP 1 (VEHICLE CONTROL)
25 26 27 28 24
GROUP 2 (TELCMER ALCOHOL)
29 -30 -31 -32 22
GROUP 3 (TELOMER ACRYLATE)
33 -34 -35 -36 22
GROUP 4 ( )
37 38 39 40 22
GROUP 5 (PFHS)
41 42 43 44 21
GROUP 6 (PFOS)
45 46 47 48
-
19
BACK TO MAIN
FC-IND - 4 0 1 - M A R - 99
1 72
BACK TO MAIN
NOTOX Project 242933
RELATIVE FOOD CONSUMPTION (G/KG BODY WEIGHT/DAY) MALES
GROUP 1 (VEHICLE CONTROL)
DAYS WEEKS CAGE
TREATMENT 1-8 1/2
1 110 2 115 3 107 4 126
8-15 2/3
94 102
99 116
15-22 3/4
89 93 99 114
22-28 4
86 90 96 101
DAYS WEEKS CAGE
RECOVERY 22-28 4
1 2 3 4
RFC-IND - 1 0 1 - M A R - 99
RECOVERY
1-8 1/2
8-14 2
14-19 . 19-22 2/3 3/4
._ -_ ___ ----
89
84 __
__
. 91 84 77 78
1 73
BACK TO MAIN
NOTOX Project 242933
RELATIVE FOOD CONSUMPTION (G/KG BODY WEIGHT/DAY) MALES GROUP 2 (TELOMER ALCOHOL)
RPC-IND - 2 0 1 - M A R - 99
DAYS WEEKS CAGE
TREATMENT
1-8 1/2
5 115 6 116 7 109 8 109
8-15 2/3
101 106
98 104
15-22 3/4
93 91 97 101
22-28 4
'
90 91 93 92
DAYS WEEKS CAGE
RECOVERY 22-28 4
5 6 7 8 72
RECOVERY
1-8 1/2
8-14 2
14-19 2/3
19-22 3/4
--
----
84 79 85 80
-- --
74
--- --
76
NOTOX Project 242933
RELATIVE FOOD CONSUMPTION (G/KG BODY WEIGHT/DAY) HALES GROUP 3 (TELOMER ACRYLATE)
DAYS WEEKS CAGE
TREATMENT
1-8 1/2
9 112 10 119 11 111 12 116
8-15 2/3
101 105 103 105
15-22 3/4
95 95 103 105
22-28 4
93 89 97 95
DAYS WEEKS CAGE
RECOVERY 22-28 4
9 --
10 11 12 74
BACK TO MAIN
RFC-IND - 3 0 1 - M A R - 99
RECOVERY
1-8 1/2
8-14 2
14-19 2/3
19-22 3/4
--
83 90
--
80 84
----- --
76
-- --
80
1 75
NOTOX Project 242933
RELATIVE FOOD CONSOMPTIQN (G/KG BODY WEIGHT/DAY) MALES GROUP 4 ( )
DAYS WEEKS CAGE
TREATMENT
1-8 1/2
13-> 113 14 112 15 107 16 1 1 1
8-15 2/3
103 102 105
99
15-22 3/4
91 93 104 98
22-28 4
90 90 100 89
DAYS WEEKS CASE
RECOVERY 22-28 4
13 --14 --15 -16 67
BACK TO MAIN
RFC-IND - 4 0 1 - M A R - 99
RECOVERY '
1-8 1/2
8-14 2
14-19 2/3
.19-22 3/4
... -- --- ---. -- 88 82 ---- --
82 73 71 72
1 76
NOTOX Project 242933
RELATIVE FOOD CONSDMPTION (G/KG BODY WEIGHT/DAY) MALES GROUP 5 (PFHS)
DAYS WEEKS CAGE
TREATMENT
1-8 1/2
8-15 2/3
17 110 101 18 122 105 19 106 99 20 108 100
15-22 3/4
91 97 102 102
22-28 4
88 95 96 94
DAYS WEEKS CAGE
RECOVERY 22-28 4
17 --18 -19 -20 73
BACK TO MAIN
RFC-IND - 5 0 1 - M AR-99
RECOVERY
1-8 1/2
8-14 ' 14-19 2 2/3
19-22 3/4
.--
--
--- --
---. _
91 83 '--
92 - 83 76 77
1 77
NOTOX Project 242933
_ ~
RELATIVE FOOD CONSUMPTION (G/KG BODY WEIGHT/DAY) MALES
GROUP 6 (PFOS)
DAYS WEEKS CAGE
x s e a s m o t t
1-8 1/2
21 120
22 114 23 1 1 1 24 113
8-15 2/3
106 102 105 103
15-22 3/4
22-28 4
94 ,
91 104 103
92 87 100
99
DAYS WEEKS CAGE
RECOVERY
22-28 4
21 22 23 --24 72
BACK TO MAIN
RFC-IND - 6 01-MAR-99
RECOVERY
1-8 1/2
8-14 2
14-19 2/3
19-22 3/4
-- --.
--
85 80 --
____
88 81 76 78
1 78
NOTOX Project 242933
RELATIVE FOOD CONSUMPTION (G/KG BODY WEIGHT/DAY) FEMALES GROUP 1 (VEHICLE CONTROL)
DAYS WEEKS CAGE
TREATMENT
1-8 1/2
25 106 26 105 27 107 28 107
8-15 2/3
104 102 103 107
15-22 3/4
98 97 104 112
22-28 4
101 94
104 109
DAYS WEEKS CAGE
RECOVERY 22-28 4
25 26 27 28 .
----
91
BACK TO MAIN
RFC-IND - 7 0 1 - M A R - 99
RECOVERY
1-8 1/2
8-14 2
14-19 2/3
19-22 3/4
.------
---
102
--
95
-- ---
-- --
107 95 86 92 .
1 79
NOTOX Project 242933
RELATIVE FOOD CONSUMPTION (G/KG BODY WEIGHT/DAY) FEMALES GROUP 2 (TELOMER ALCOHOL)
DAYS WEEKS CAGE
TREATMENT
1-8 1/2
29 104 30 110 31 96 32 105
8-15 2/3
102 108 105 106
15-22 3/4
98 100 110 111
22-28 4
97 102 101 108
DAYS WEEKS CAGE
RECOVERY
22-28 4
29 --30 --
31 --32 86
BACK TO MAIN
RFC-IND - 8 0 1 - M A R - 99
RECOVERY
1-8 1/2
8-14 2
14-19 2/3
19-22 3/4
__ ___
102 93 106 98
88
92
1 80
NOTOX Project 242933
-
RELATIVE FOOD CONSUMPTION (G/KG BODY WEIGHT/DAY) FEMALES
GROUP 3 (TELOMER ACRYLATE)
. TREATMENT
DAYS WEEKS CAGE
1-8 1/2
8-15 . 15-22 2/3 3/4
33 105 100 100 34 117 110 107 35 108 110 112 36 110 109 112
22-28 4
101 107 . 105 107
AYS WEEKS CAGE
RECOVERY 22-28 '4
'
33 --34 --35 --36 90
BACK TO MAIN
RFC-IND - 9 01-MAR-99
*
. RECOVERY
1-8 1/2
8-14 2
14-19 2/3
19-22 3/4
-- -- __
_
105 96
----
109 98 88 87
1 81
NOTOX Project 242933
RELATIVE FOOD CONSUMPTION (G/KG BODY WEIGHT/DAY) FEMALES GROUP 4 ( )
DAYS WEEKS CASE
TREMMENT
1-8 1/2
37 104 38 110 39 115 40 104
8-15 2/3
104 102 113 107
15-22 3/4
98 102 112 106
22-28 4
100 100 106 100
DAYS WEEKS CASE
RECOVERY 22-28 4
'
37 -38 -39 --40 81
'
BACK TO MAIN
R F C - I N D - 10 0 1 - M A R - 99
RECOVERY
1-8 1/2
8-14 2
14-19. 2/3
19-22 3/4
--------
106 98
-- ----- ---
97 --
--
91 81 83
1 82
NOTOX Project 242933
RELATIVE FOOD CONSUMPTION {G/KG BODY WEIGHT/DAY) FEMALES GROUP 5 (PFHS)
DAYS WEEKS CAGE
TBSKTMBSV
1-8 1/2
41 110 42 130 43 102 44 109
8-15 2/3
100 118 105 113
15-22 3/4
100 111 107 111
22-28 4
103 105 102 106
DAYS WEEKS CAGE
RECOVERY 22-28 4
41 --
42 -43 -44 84
BACK TO MAIN
RFC-IND - 11 0 1 - M A R - 99
RECOVERY
1-8 . 1/2
8-14 2
14-19 2/3
19-22 3/4
-- __ ___ ___ 107 97 105 98 84 88
1 83
NOTOX Project 242933
RELATIVE FOOD CONSUMPTION (G/KG BODY WEIGHT/DAY) FEMALES
GROUP 6 (PFOS)
DAYS WEEKS CAGE
TREATMENT
1-8 1/2
45 46 47 48
125 104 100
115
8-15 2/3
106 97
100 111
15-22 3/4
101 92 96
110
22-28 4
95 92 93 102
DAYS WEEKS CAGE
RECOVERY 22-28 4
45 -46 -47 -48 85
BACK TO MAIN
RFC-IND - 12 0 1 - M A R - 99
RECOVERY
1-8 1/2
8-14 2
14-19 2/3
19-22 3/4
. . . __ __
97 90 103 95 85
86
184
BACK TO MAIN
NOTOX Project 242933
`
TELOMER ALCOHOL/TELOMER ACRYLATE/
7PFHS/PF0S
KEY-IND - 1 22-MAR-99
KEY TO MISSING VALUES AND REMARKS HAEMATOLOGY / CLINICAL BIOCHEMISTRY
AFTER 4 WEEKS
Animal 1, 2, 3, 4, 56 81, 84, 97, 98, 99, 114, 129, 145, 164, 177, 179, 180,
Animal 1, 33, 55, 59, 65, 72, 77, 78. 79, 89, 132, 145, 162, 168,
Animal 62, 174 Animal 129
--- = EDTA sample clotted
-- = Citrate sample clotted
= PTT in plasma not reproducible = deviating curve noted for PTT 1n plasma
Animal 2, 17, 18, 19, 25, 36, 40, 49, 50, 66, 67, 71, 72, 74, 79, 87, 88, 89, 90
Animal 46 and 59
Serum sample showed haemolysis = ALAT in serum not reproducible
AFTER 2 WEEKS RECOVERY Animal 75, 169, 186 AFTER 4 WEEKS RECOVERY Animal 14, 29, 61, 78 Animal 127
. Citrate sample clotted
= Citrate sample (partly) clotted --- = ALAT in serum not reproducible
NOTOX Project 242933
HAEMATOLOGY AFTER 4 WEEKS MALES GROUP 1 (VEHICLE CONTROL)
BACK TO MAIN
HEM-IND - 1 30-MAR-9 9
ANIMAL NUMBER
HAEMATOLOGY PARAMETERS
RBC HB
HCT
T/l
Bmol/1
1/1
1 2
3
4 5 7.52 6 6.83 7 7.25 8 7.01 9 6.85 10 7.11 11 6.91 13 6.97 14 .7.29 15 6.80
9.7 9.2 9 .-6 9.4 8.9 9.0 9.0 8.6
9.2 8.7
0.423 0.406 0.415 0.411 0.403 0.413 0.406 0.390 0.418 0.389
mcv 1
56.3 59.4 57.2 58.6 58.8 58.1 58.8 56.0 57.3 57.2
MCH fmol
MCHC itmol/1
REW %
1.290 1.347 1.324 1.341 1.299 1.266 1.302 1.234 1.262 1.279
22.9 22.7 23.1 22.9 22.1
21.8 22.2 22.1
22.0 22.4
12.3 12.2
12.2 11.6
12.4 12.4 12.4 13.4 12.3 12.5
PLATELETS G/l
1329 1182 1340 1085 1237
988 1187 1074 1189 1204
ANIMAL NUMBER
1 2 3 4 5 6 7 8 9 10 11 13 14 15
HAEMATOLOGY PARAMETERS
WBC
SEG.
EO.
G/l 1
1
BASO. 1
--
--
10.9 17.6 14.1 16.2
8.8 6.6 9.9 10.9 9.3 9.0
--
-- --
0.105 0.140 0.075 0.090 0.190 0.145 0.110 0.145 0.120 0.120
--
-- --
0.010 0.010 0.000 0.005 0.015 0.005 0.010 0.000 0.010 0.005
--
-- ---
0.000 0.000 0.000 . 0.000 0.000 0.000 0.000 0.000 0.000 0.000
MONO. 1
--
-- ... 0.025 0.030 0.030 0.030 0.010 0.020 0.030 0.025 0.030 0.025
LYMPH. 1
--
--- 0.860 0.820 0.895 0.875 0.785 0.830 0.850 0.830 0.840 0.850
FT SEC
15.5 15.6 14.7 14.2 15.2 14.3 14.5 14.7 14.8 14.9 14.3 16.2 14.7
FTT SEC
15.5 16.2 17.2 16.7 16.3 ' 17.5 16.1 14.9 16.0 15.7 15.7 14.8 16.4
NOTOX Project 242933
HAEMATOLOGY
.
AFTER 4 WEEKS
MALES
GROUP 2 (TELOMER ALCOHOL)
BACK TO MAIN
HEM-IND - 2 3 0 - M A R - 99
ANIMAL NUMBER
17
ia
19 20 21 22 23 24 25 26 27 29 30 31
HAEMATOLOGY PARAMETERS
RBC HB
HCT
T/l
irmol/1
1/1
6.19
8.3
0.386
6.36 8.5 .0.371
6.21
8.4
0.368
6.49
8.2
0.377
6.70
9.0
0.403
6.76
8.9
0.391
7.09
9.2
0.405
6.93
9.2
0.407
6.83
8.9
0.399
6.93
9.1
0.411
7.02
8.9
0.404
7.21
9.5
0.425
6.51
8.7
0.389
6.80
8.6
0.391
mcv fl
62.4 58.3 59.3 58.1 60.1 57.8 57.1 58.7 58.4 59.3 57.5 58.9 59.8 57.5
MOT fmol
1.341 1.336 1.353 1.263 1.343 1.317 1.298 1.328 1.303 1.313 1.268 1.318 1.336 1.265
M2HC imol/1
21.5 22.9 22.8 21.8 22.3 22.8 22.7 22.6 22.3 22.1 22.0 22.4 22.4 22.0
ROM %
10.7 11.5 12.4 11.2 12.0 12.2 1 1 .1 12.4 12.5 12.7 11.7 12.7 13.0 11.7
PLATELETS G/l
842 928 929 913 1111 1216 992 1047 1119 897 984 1051 1140 1184
ANIMAL
NUMBER
17 18 19 20 21 22 23 24 25 26 27 29 . 30 31
HAEMATOLOGY PARAMETERS
NBC
SB3.
BO.
G/l 1
1
10 .1
7.2 6.4 7.3 10.4 12.3 8.6 10.9 10.9 11.7 10.5 8.6
12.5 11.9
0.085 0.085 0.190
0.155 0.105 0.115 0.120
0.080 - 0.130
0.105 0.100
0.225 0.130 0.130
0.000
0.025 0.005 0.015 0.000
0.015 0.005 0.010
0.015 0.040 0.000 0.010
0.015 0.005
BASO. 1
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
MONO.
1
0.015 0.015 0.015 0.040 0.015 0.040 0.020 0.010 0.025 0.005 0.035 0.040 0.015 0.015
LYMPH.
1
PT SEC
0.900 0.875 0.790 0.790 0.880 0.830 0.855 0.900 0.830 0.850 0.865 0.725
0.840 0.850
15.8 14.8 ' 15.6 14.5 14.4
14.5 15.0 15.4 13.9 15.3 14.8 15.7 15.3 14.5
PTT SEC
16.0 14.9 15.4 16.4 15.8 16.9 14.6 16.6 15.5 16.5 16.2 17.1 15.9 15.8
'
1 87
NOTOX Project 242933
HAEMATOLOGY AFTER 4 WEEKS MALES GROUP 3 (TELOMER ACRYLATE)
BACK TO MAIN
HSM-IND - 3 3 0 - M A R -9 9
ANIMAL NUMBER
33 34 35 36 37 38 39 40 41 42 43 45 46 47
HAEMATOLOGY PARAMETERS
RBC HB
HOT
T/l
ntnol/1
1/1
6.12
6.50 6.36 6.43 6.76 6.83 6.55 7.16 6.49 6.34 6.83 6.73 6.56
6.82
8.2
8.5 8.0
8.2 9.0 9.2 8.5 9.4 8.7
8.3 9.0 8.9 8.9 9.0
0.367 0.382 0.372 0.380 0.397
0.402 0.372 0.409 0.387
0.374 0.409 0.386 0.390 0.403
rocv fl
60.0 58.8 58.5 59.1 58.7 58.9 56.8 57.1 59.6 59.0 59.9 57.4 59.5 59.1
MCH fmol
1.340 1.308 1.258 1.275 1.331 1.347 1.298 1.313 1.341 1.309 1.318 1.322 1.357 1.320
MCHC tnnol/1
22.3 22.3 21.5 21.6 22.7 22.9 22.8 23.0 22.5 22.2 22.0 23.1 22.8 22.3
REW %
12.2 11.5 11.5 11.5 12.7 12.3 12.5 11.6 13.1 12.4 11.8 1 2 .1 1 2 .1 13.4
PLATELETS G/l
1244 957
1063 930
1388 1354 1191 1114 1099 1137
944 1268
904 1022
ANIMAL NMBER
33 34 35 36 37 38 39 40 41 42 43 45 46 47
HAEMATOLOGY PARAMETERS
WBC
SEG.
EO.
G/l 1
1
11.8
9.9 9.1 11.2 13.4 9.4
9.6 9.3 11.2
11.8 8.9
13.8 6.7 6.8
0.170 0.155 0.080 0.140 0.100
0.065 0.090 0.080 0.095 0.090 0.120 0.145
0.155 0.150
0.005 0.010 0.000
0.015 0.005 0.005 0.020 0.005 0.005 0.000
0.005 0.005 0.005 0.010
BASO. 1
tONO. 1
0.000 0.000
0.000
0.000 0.000
0.000
0.000 0.000 0.000
0.000 0.000 0.000
0.000 0.000
'
0.025 0.020
0.015 0.020 0.020
0.010 0.020 0.020
0.015 0.025 0.020 0.020
0.045 0.000
LYMPH. 1
0.800 0.815 0.905 0.825 0.875 0.920 0.870 0.895 0.885 0.885 0.855 0.830 0.795 0.840
PT SEC
15.6 15.0 15.3 13.9 14.3 14.7 15.6 15.1 13.9 15.5 15.5 14.4 14.8
PIT SEC
15.5 17.4 23.8 17.1 16.5 18.7 15.5 16.2 15.9 16.7 15.3 16.1 17.8
1 88
NOTOX Project 242933
HAEMATOLOGY AFTER 4 WEEKS MALES GROUP 4 ( )
.
BACK TO MAIN
HEM-IND - 4 3 0-MAR-99
.. : .
ANIMAL NUMBER
49 50 51 52 53 54 55
57 58 59 61 62 63
HAEMATOLOGY PARAMETERS
RBC HB
HOT
T/l
nntol/1
1/1
mcv fl
7.07 6.54 6.25 6.62 6.65 7.18 7.13
8.7 8.2
7.6 8.5 8.7 9.6 9.4
0.406 0.371
0.358 0.387 0.384 0.429 0.418
57.4 56.7 57.3 58.5 57.7 59.7 58.6
6.42
8.7
0.386 60.1
6.48
8.7
0.389
60.0
7.21
9.2
.0.418
58.0
6.82
8.9
0.402
58.9
6.94
9.4
0.418
60.2
6.90
8.8
0.395
57.2
MCH fmol
1.231 1.254 1.216 1.284 1.308 1.337 1.318
1.355 1.343 1.276 1.305 1.354 1.275
MCHC irmol/1
21.4 22.1 21.2 22.0 22.7 22.4 22.5
22.5 22.4 22.0 22.1 22.5 22.3
RDW
11.7 11.5 11.7 11.7 11.9 11.7 1 2 .1
12.2 11.9 12.5 12.4 1 2 .1 1 2 .1
PLATELETS G/l
1063 1149 1030 1072 1462
874 876
1084 1084
860 . 1049
932 1121
ANIMAL NUMBER
49 50 51 52 53 54 55 56 57 58 59 61 62 63
HAEMATOLOGY PARAMETERS
WBC
SEG.
ED.
G/l 1
1
BASO. 1
MEMO. 1
12.4 15.2 14.3 14.5 10.6
8.2 10.8 --
14.1 10.4 12.2
9.6 11.7
7.6
.
0.100
0.105 0.070 0.085 0.065 0.055
-0-.-110
0.095 0.100 0.075 0.055 0.140 0.070
0.010
0.005 0.000 0.010 0.010
0.005 0.010 ---
0.000 0.010 0.010 0.005 0.005 0.015
0.000 0.000 0.000 0.000 0.000 0.000
0.000 --
0.000 0.000 0.000 0.000 0.000 0.000
0.025 0.020 0.020 0.010 0.015 . 0.020 ' .040 --- . "
0.035 0.015 0.030 0.020 0.035 0.015
LYMPH. 1
0.865 0.870 0.910 0.895 0.910 0.920 0.840 --0.870 0.875 0.885 0.920 0.820 0.900
ET SEC
14.9 14.9 13.7 15.4 15.2 14.1 -- 13.8 13.8 13.8 --14.8 14.1 14.5
PIT SEC
15.7 15.2 16.0 19.0 17.4 19.5 -- 26.8 16.7 16.7 -- 19.5 -- . 18.1
1 89
NOTOX Project 242933
HAEMATOLOGY AFTER 4 WEEKS MALES GROUP 5 (PFHS)
BACK TO MAIN
HEM-IND - 5 30-MAR-9 9
ANIMAL NUMBER
HAEMATOLOGY PARAMETERS
RBC KB
HCT
T/l
mmol/1
1/1
65 66
67
68 69 70 71 72 73 74 75 77 78 79
6.14 5.71 5.55 6.50 7.04 7.06 7.47 6.40 6.64 6.72 6.65 '6.35 6.95 6.80
8.0
7.5 7.6 8.1
9.1 9.0 9.7
8.4 8.5 8.7 8.5 8.5 8.6 9.0
0.357 0.347 0.347 0.365 0.395 0.409 0.442 0.371 0.388
0.388 0.380 0.378 0.395 0.404
mcv fl
58.1 60.8 62.5 56.2 56.1 57.9 59.2 58.0 58.4 57.7 57.1 59.5 56.8 59.4
MCH fmol
1.303 1.313 1.369 1.246 1.293 1.275 1.299 1.312 1.280 1.295 1.278 1.339 1.237 1.324
M2HC itmol/1
22.4 21.6 21.9 22.2 23.0 22.0 21.9 22.6 21.9 22.4 22.4 22.5 21.8 22.3
ROT %
11.8 1 1 .1 13.1 11.0 1 1 .1 11.6 12.2 11.8 12.6 12.3 12.0 12.8 12.2 12.8
PLATELETS G/l
1017 929 946 969
1074 977
1011 873 997 943
1092 1031
221 105
ANIMAL NUMBER
65 66 67 68 69 70 71 72 73 74 75 77 78 79
HAEMATOLOGY PARAMETERS
WBC
SBG.
BO.
G/l 1
1
BASO. 1
H3NO. 1
7.3 8.1
11.1 9.2 7.1
13.6 14.2 1 2 .1
13.9 8.7 6.4
11.0
6,0 6.5
0.175 0.255 0.185 0.125 0.085 0.170 0.140
0.115 0.220 0.100
0.130 0.105 0.160 0.105
0.005
0.000
0.020
0.000
0.010
0.000
0.000
0.000
0.000
0.000
0.010 ' 0.000
0.000
0.000
0.010
0.000
0.005
0.000
0.020
0.000
0.000
0.000
0.015
0.000
0.005
0.000
0.015
0.000
0.030 0.020
0.015 0.035 0.020
0.025 0.040 0.025 0.050 0.025 0.025 0.040 0.005 0.005
LYMPH. 1
0.790 0.705 0.790 0.840 0.895 0.795 0.820 0.850 0.725 0.855 0.845 0.840 0.830 0.875
PT SEC
13.9 14.4 14.1 14.1 13.5
1--4-.-7
14.7 13.7
1--3-.7 ---
--
PIT SEC
15.9 15.8 15.8 17.1 17.4 18.1
--
17.3 17.4.
.1-6-.7
--
--
.
190
NOTOX Project 242933
HAEMATOLOGY AFTER 4 WEEKS MALES GROUP 6 (PFOS)
BACK TO MAIN
HEM-IND - 6 30-MAR-9 9
ANIMAL NUMBER
81 82 83 84 85 86 87 88 89 90 91 93 94 95
HAEMATOLOGY PARAMETERS
RBC HB
HOT
T/l
nmol/l
1/1
--
6.44 6.20 --
6.36 7.37 6.95 6.15 6.44 6.48 6.55 7.13 7.02 6.82
--
8.7 8.2 --
8.9 9.4 9.6 8.3 9.0 8.7 8.9 8.9 9.1 9.1
0.377 0.361
0.395 0.429 0.414 0.367 0.395 0.382 0.390 0.389 0.409 0.398
mcv fl
58.5 58.2
62.1 58.2 59.6 59.7 61.3 59.0 59.5 54.6 58.3 58.4
MCH fmol
M2HC `mnol/1
ROW %
PLATELETS G/l
1.351 1.323
1.399 1.275 1.381 1.350 1.398 1.343 1.359 1.248 1.296 1.334
23.1 22.7
22.5 21.9 23.2 22.6 22.8 22.8 22.8 22.9 22.2 22.9
11.3 11.5
11.2 12.7 11.3 13.7 11.5 12.3 12.2 12.7 11.8 11.5
1120 1150
1031 935
1109 1239 1019 1114 1111 1127 ' 1071 1082
ANIMAL NUMBER
81 82 83 84 85 86 87 88 89 90 91 93 94 95
HAEMATOLOGY PARAMETERS
WBC
SEE.
EO.
G/l 1
1
7.5
-1-0-.2
11.3 10.8 12.3
8.4 13.2 14.2 10.4
7.4 10.2 12.9
___
0.110 0.095 ---
0.075 0.165 0.075 0.190 0.080 0.145 0.075 0.095 0.115 0.145
___
0.000 0.010 --
0.015 0.005 0.005 0.005 0.005 0.005 0.000 0.005 0.000 0.000
BASO. 1
--
0.000 0.000 -- 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
MONO. 1
0.025 0.020
0.035 0.010 0.015 0.025 0.015 0.035 0.010 0.030 0.025 0.035
LYMPH. 1
0.865 0.875 -- * 0.875 0.820 0.905 0.780 0.900 0.815 0.915 0.870 0.860 0.820
FT SEC
14.1 14.8 15.6 15.4 14.7 14.4 14.5' 14.1 --15.6 14.7 14.6 14.5 13.8
PTT SEC
20.8 15.2 20.0 15.0 16.9 16.1 19.2 15.9 -- ' 17.1 16.4 18.0 17.7 15.7
1 91
N O T O X Project 242933.
HAEMATOLOGY AFTER 4 WEEKS FEMALES GROUP 1 (VEHICLE CONTROL)
BACK TO MAIN
HEM-IND - 7 3 0 - M A R - 99
ANIMAL NUMBER
97 98 99 100 101 102 103 104 105 106 107 109 110 111
HAEMATOLOGY PARAMETERS
RBC HB
HCT
T/l
nmol/1
1/1
--
--
--
6.50 6.66 6.80 6.82 7.02 6.78 6.79 6.68 6.54 6.63 6.73
--
--
--
8.9 9.5 9.0 9.2 9.6 8.9 9.2 8.7 8.7 9.0 9.1
0.376 0.292 0.369 0.379 0.389 0.363
0.389 0.369 0.356 0.392 0.380
rocv fl
57.8 58.9 54.3 55.6 55.4 53.5 57.3 55.2 54.4 59.1 56.5
MCH fmol
III
1.369 1.426 1.324 1.349 1.368 1.313 1.355 1.302 1.330 1.357 1.352
M2HC mrrol/l
23.7 24.2 24.4 24.3 24.7 24.5 23.7 23.6 24.4 23.0 23.9
ROW PLATELETS % G/l
"
10.9 11.2
11.5 11.1
11.6 11.5 10.9 11.9 11.4 11.6 10.5
1271 1114
1324 1050 1302 1335 1078 1214 1019
996 ' 1121
ANIMAL NUMBER
97 98 99 100 101 102 103 104 105 106 107 109 110 111
HAEMATOLOGY PARAMETERS
NBC
SEG.
EO.
G/l 1
1
...
--
--
7.0 11.1
7.0 9.8 9.5 9.0 6.3 4.9 5.2 5.9 5.5
--
~ --
0.080 0.060 0.070 0.045 0.070 0.060 0.090 0.130 0.075 0.095 0.125
___
--
---- --
0.015 0.000 0.010 0.005 0.015 0.010 0.005 0.025 0.010 0.010 0.000
BASO. 1
___
--
---- --
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
MONO. 1
___ -- ...
0.020 0.040 0.045 0.010 0.020 0.015 0.020 0.040 0.025 0.025 0.020
LYMPH. 1
-- ------
0.885 0.900 0.875 0.940 0.895 0.915 0.885 0.805 0.890 0.870 0.855
PT SEC
14.2 13.9 13.9 14.3 16.5 17.3 15.6 15.5 16.0 15.3 15.9 16.0 15.1 15.4
FIT SEC
14.2 17.7 16.1 18.2 17.0 14.1 17.5 16.3 17.0 16.1 14.1 15.5 14.6 14.7
;
NOTOX Project 242933
HAEMATOLOGY AFTER 4 WEEKS FEMALES GROUP 2 (TELOMER ALCOHOL)
BACK TO MAIN
HEM-IND - 8 3 0 - M A R - 99
ANIMAL NUMBER
113
115 116 117 118 119 120 121 122 123 125 126 127
HAEMATOLOGY PARAMETERS
RBC HB
HCT
T/l
mraol/l
1/1
.
mcv fl
5.44
5.59 6.52 6.28 6.76 6.51 6.21 6.00 6.61 6.29 6.50 6.55 6.29
7.2
7.7 8.7 8.9 9.2 9.0 8.6 8.3 8.6 8.2 8.7 8.8 8.7
0.312
0.331 0.376 0.362 0.372 0.365 0.353 0.340 0.356 0.348 0.363 0.369 0.367
57.4
59.2 57.7 57.6 55.0 56.1 56.8 56.7 53.9 55.3 55.8 56.3 . 58.3
MCH mol
1.324
1.377 1.334 1.417 1.361 1.382 1.385 1.383 1.301 1.304 1.338 1.344 1.383
MCHC . nrnol/1
23.1
23.3 23.1 24.6 24.7 24.7 24.4 24.4 24.2 23.6 24.0 23.8 23.7
REW %
10.8
10.8 11.2 11.2 11.2 10.7 11.2 11.0 11.8 11.6 1 2 .1 11.9 10.7
PLATELETS G/l
1041
1114 970
1134 1463 1275 1262 1283 1097 1016 ' 1165 1095
920
A N IM AL NUMBER
113 114 115 116 117 118 119 120 121 122 123 125 126 127
HAEMATOLOGY PARAMETERS
WBC
SEG.
EO.
G/l 1
1
8.9
0.135
0.000
------
6.9
0.110
0.020
8.8
0.040
0.000
5.6
0.175
0.005
8.5
0.060
0.005
6.8
0.105
0.005
8.3
0.160
0.005
9.8
0.075
0.005
4.2
0.115
0.000
8.6
0.140
0.000
7.8
0.065
0.000
5.4
0.085
0.005
8.2
0.070
0.000
BASO. 1
0.000 -- 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
MONO. 1
0.000 -- 0.035 0.010 0.025 0.040 0.010 0.025 0.025 0.015 0.020 0.025 0.025 0.010
LYMPH. 1
0.865 -- 0.835 0.950 0.795 0.895 0.880 0.810 0.895 0.870 0.840 0.910 0.885 0.920
PT SEC
13.8 14.6 14.4 14.1 15.3 15.6 15.8 15.3 15.1 15.2 14.7 15.8 15.3 15.6
PTT SEC
16.2 16.4 14.5 15.2 15.5 16.4 15.0 15.1 13.5 15.0 14.9 13.8 14.6 14.2
1 S3
NOTOX Project 242933
-
HAEMATOLOGY
.
AFTER 4 WEEKS
FEMALES
GROUP 3 (TELOMER ACRYLATE)
BACK TO MAIN
HEM-IND - 9 30-MAR -99
.
ANIMAL NUMBER
129 130 131 132 133 134 135 136 137 138 139 141 142 143
HAEMATOLOGY PARAMETERS
RBC HB
HOT
T/l
imol/l
1/1
7.23 5.98 6.30 6.69 6.36 6.82 6.48 6.54 6.70 6.90 6.83 6.86
6.22
9.4
7.5 8.1 8.9 8.9 9.6 8.7 9.0
8.8 8.8
8.9 9.0 8.6
0.408 0.327 0.360 0.372 0.370 0.388 0.359 0.379 0.362 0.373 0:366 0.378 0.363
mcv fl
56.4 54.7 57.1 55.6 58.2 56.9 55.4 58.0 54.0 54.1 53.6 55.1 58.4
MCH fimi . . ' \
MCHC imol/1
--
1.300 1.254 1.286 1.330 1.399 1.408 1.343 1.376 1.313 1.275 1.303 1.312 1.383
.
23.0 22.9 22.5 23.9 24.1 24.7 24.2 23.7 24.3 23.6
24.3 23.8 23.7
RDM %
11.3 12.0 11.0 11.5 10.9 10.7 10.8 10.5 12.2 12.6 11.5 11.6 11.1
PLATELETS G/l
1136 1004 1089 1082 1383 1304 1180 1062 1247 1068 1317 1040 1029
ANIMAL NUMBER
HAEMATOLOGY PARAMETERS
WBC
SEG.
EO.
G/l 1
1
129
130 5.3 131 6.4 132 6.8 133 . 5.7 134 8.2 135 6.6 136 5.4 137 8.2 138 6.4 139 4.2 141 13.2 142 7.9 143 4.7
0.130
0.195 0.070 0.085 0.110 0.110 0.075 0.095 0.090 0.095 0.120
0.070 0.175
--
0.005 0.005 0.000
0.000
0.000
0.015 0.005 0.030 0.020 0.015 0.005 0.015 0.005
BASO. 1
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0 .000
MONO. 1
0.025 0.015 0.020 0.015 0.005 0.015 0.010 0.030 0.025 0.000 0.030 0.015 0.015
LYMPH. 1
0.840 0.785 0.910 0.900 0.885 0.860 0.910 0.845 0.865 0.890 0.845 0.900 0.805
PT SEC
14.7 13.8 13.7
--
14.4 15.6 15.9 15.9 16.0 14.9 15.4 15.3 15.5 14.1
PTT SEC
16.0 16.3 -- . 17.1 15.5 16.4 16.0 16.3 14.7 14.7 14.7 14.6 15.6
1 S4
NOTOX Project 242933
HAEMATOLOGY AFTER 4 WEEKS FEMALES GROUP 4 ( )
---
'
BACK TO MAIN
HEM-IND - 10 3 0-MAR-99
*
ANIMAL NUMBER
145 146 147 148 149 150 151 152 153 154 155 157 158 159
HAEMATOLOGY PARAMETERS
RBC HB
HOT
T/l
mnol/1
1/1
6.23 6.64 6.45 6.66
6.07 6.86
6.64 6.79 6.99 6.22
7.33 6.86
6.33
8.9 9.0 8.7 9.6 8.4 9.4 9.2
8.7 9.4 8.6 9.4 8.9
9.0
0.380 0.388 0.363
0.392 0.347 0.385 0.383 0.378 0.382 0.363 0.402 0.375 0.368
mcv MCH fl fmol
61.0
58.4 56.3
58.9 57.2 56.1 57.7 55.7 54.6 58.4 54.8 54.7
58.1
.
1.429 1.355 1.349
1.441 1.384 1.370 1.386 1.281 1.345 1.383 1.282 1.297 1.422
MCHC nraol/l
RLW %
23.4 23.2 24.0
24.5 24.2 24.4 24.0 23.0 24.6 23.7 23.4 23.7
24.5
11.5 11.5 11.5 11.3
11.3 11.4 10.5 11.9
11.4 11.0
11.4 11.3 11.0
PLATELETS G/l
1194 1032 1102 1172 1276 1134 1136 1137 1177 1169 1176 1332 1165
ANIMAL NUMBER
145 146 147 148 149 150 151 152 153 154 155 157 158 159
HAEMATOLOGY PARAMETERS
WBC
SEG.
EO.
G/l 1
1
1 0 .1 7.1
9.1 8.3 4.9 9.8 6.5 5.8 7.8 4.7 8.9 9.0 6.9
0.075 0.080 0.075
0.090 0.065
0.075 0.080 0.145 0.105 0.125 0.070 0.070 0.080
0.005 0.005 0.005 0.010 0.020
0.005 0.010
0.015 0.015 0.015 0.020
0.005 0.015
BASO. 1
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
MONO. 1
0.020 0.015 0.030 0.010 0.025 0.035 0.025 0.025 0.030 0.040 0.025 0.020 0.015
LYMPH. 1
PT SEC
0.900 0.900 0.890
0.890 0.890 0.885 0.885 0.815 0.850 0.820
0.885 0.905 0.890
14.3 14.8
13.5 14.5 14.4 14.7 14.9 14.6 14.4 13.8 15.5 14.4 14.6
PIT SEC
16.8 16.6 16.4 17.5 17.5 18.2 17.6 16.2 15.5 15.7 16.6 17.4 18.7
1 95
NOTOX Project 242933
HAEMATOLOGY AFTER 4 WEEKS FEMALES GROUP 5 (PFHS)
-
.
BACK TO MAIN
HEM-IND - 11 30-MAR-99
.
ANIMAL CLUMBER
HAEMATOLOGY PARAMETERS
RBC HB
HOT
T/l
nmol/1
1/1
161 5.92 162 6.55 163 . 6.70 165 6.23 166 6.58 167 6.65 168 6.36 169 6.07 170 6.42 171 6.50 173 6.63 174 5.94 175 6.02
8.4 9.0 8.7
8.6 9.0 8.8 9.1 8.5 8.4 9.1 8.5 8.7 7.8
0.356 0.375 0.366
0.353 0.374 0.360 0.374 0.333 0.342 0.381 0.350 0.359 0.325
mcv fl
60.1 57.3 54.6
56.7 56.8 54.1 58.8 54.9 53.3 58.6 52.8 60.4 54.0
MCH fmol
' 1.419 1.374 1.299
1.380 1.368 1.323 1.431 1.400 1.308 1.400 1.282 1.343 1.296
MCHC ntnol/1
REW %
23.6 24.0 23.8
24.4 24.1 24.4 24.3 25.5 24.6 23.9 24.3 24.8 24.0
10.9 10.9
11.8
11.7 11.0 11.9 10.6 11.3 10.9 10.7 11.6 10.6 12.7
PLATELETS G/l
1112 314 975
1192 1178 1044 1120 1053 1066 1157 1019
938 1281
ANIMAL NUMBER
161 162 163 164 165 166 167 168 169 170 171 173 174 175
HAEMATOLOGY PARAMETERS
WBC
SEG.
BO.
G/l 1
1
6.3 4.8 5.2
--
7.2 10.8
5.8 9.1 4.6 6.7 5.5 4.5 4M 7.2
0.065 0.050
0-.-065
0.040 0.040 0.090 0.065 0.115 0.110
0.045 0.095 0.155 0.100
0.005 0.005 0.015
--
0.010 0.010 0.020 0.005 0.005 0.010 0.005 0.020 0.005 0.005
BASO. 1
0.000 0.000 0.000
--
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
MONO. 1
0.020 0.010 0.005
--
0.045 0.035 0.035 .015 0.035 0.015 0.020 0.025 0.035 0.020
LYMPH. 1
0.910 0.935 0.915
--
0.905 0.915 0.855 0.915 0.845 0.865 0.930 0.860 0.805 0.875
PT SEC
13.7
--
14.0 14.3 14.1 13.9 15.8
--
15.9 14.4 14.2 14.4 15.1 14.0
PTT SEC
15.8 * -----
15.7 15.1 16.4 17.9 2 2 .1
--
16.0 15.5 18.1
1-5-.9
16.8
. '
1 96
NOTOX Project 242933
HAEMATOLOGY AFTER 4 WEEKS FEMALES GROUP 6 (PFOS)
BACK TO MAIN
HEM-IND - 12 3 0 - M A R - 99
ANIMAL NUMBER
177 178 179 180 181 182 183 184 185 186 187 189 190 191
HAEMATOLOGY PARAMETERS
RBC HB
HOT
T/l
tnnol/1
1/1
mcv fl
6.69
6.54 6.30 7.23 6.51 6.38 5.98 6.66 6.07 5.95 6.21
8.6
8.9 8.6 9.6 9.0 8.5 8.0 8.9 8.1 8.6 8.2
0.360
53.8
0.369 0.356 0.399 0.369
0.354 0.334 0.371 0.338 0.3610.333
56.4 56.5 55.2 56.7 55.5 55.9 ' 55.7 55.7 60.7
53.6
MCH fnol
MCHC itmol/1
RDW %
1.286
1.361 1.365 1.328 1.382 1.332 1.338 1.336 1.334 1.445 1.320
23.9
24.1 24.2 24.1 24.4 24.0 24.0 24.0 24.0 23.8 24.6
11.7
10.7 10.9 11.2 10.4 12.0 12.0 11.3 11.1 10.7 11.3
PLATELETS G/l
988
1068 1220 1027 1208 1229 1187 1069 1423 1033 1024
ANIMAL NUMBER
177 178 179 180 181 182 183 184 185 186 187 189 190 191
HAEMATOLOGY PARAMETERS
WBC
SEG.
EO.
G/l 1
1
4.5
--
--
7.4 11.8 1 1 .1
8.3 7.2 6.6 6.7 7.9 8.3 1 2 .1
0.055
--
--
0.075 0.080 0.040 0.085 0.090 0.120 0.080 0.070 0.080 0.075
___
0.000
--
--
0.005 0.005 0.005 0.005 0.000 0.010 0.010 0.000 0.010 0.005
BASO. 1
0.000
--
--
0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
MONO. 1
LYMPH. 1
--
0.010
--
0.015 0.020 0.025 0.025 0.010 0.030 0.025 0.020 0.025 0.015
0.935
--
---- -
0.905 0.895 0.930 0.885 0.900 0.840 0.885 0.910 0.885 0.905
FT SEC
13.3 13.9 14.8 14.7 14.2 14.7 14.3 14.3 14.8 14.4 14.7 14.1 14.7 14.5
PTT SEC
13.6 12.9 16.7 14.6 16.8 17.7 20.0 20.5 15.0 16.2 15.8 16.4 16.0 18.1
1 97
NOTOX Project 242933
HAEMATOLOGY
.
AFTER 2 WEEKS RECOVERY
MALES GROUP 1 (VEHICLE CONTROL)
BACK TO MAIN
H E M - I N D - 13 30-MAR-99
ANIMAL NUMBER
9 10 11
HAEMATOLOGY PARAMETERS
RBC HB
HCT
T/l
ntnol/1
1/1
7.05 7.41 7.23
8.7 9.2 9.4
0.406 0.428 0.417
mcv fl
57.6 57.8 57.7
MCH fmol
1.234 1.242 1.300
MCHC mnol/1
21.4 21.5 22.5
RDW %
14.1 14.5 13.6
PLATELETS G/l
1293 1086 1152
ANIMAL NUMBER
9 10 11
HAEMATOLOGY PARAMETERS
WBC
SEG.
ED.
G/l 1
1
8.1 6.9 10.5
0.150 0.120 0.065
0.010 0.015 0.015
BASO. 1
0.000 0.000 0.000
MONO. 1
LYMPH. 1
0.030 0.025 0.025
0.810 0.840 . 0.895
PT SEC
14.6 14,3 15.0
PIT SEC
17.4 15.3 15.9
1 98
NOTOX Project 242933
HAEMATOLOGY
.
AFTER 2 WEEKS RECOVERY
MALES
GROUP 2 (TELOMER ALCOHOL)
BACK TO MAIN
H E M - I N D - 14 3 0 - M A R - 99
ANIMAL NUMBER
HAEMATOLOGY PARAMETERS
RBC HB
HOT
T/X
imol/1
1/1
25 . 6.79 26 7.06 27 7.28
8.8
8.9 8.9
0.395 0.407 0.409
mcv fl
58.2 57.6 56.2
M2H fmol
1.296 1.261 1.223
MCHC mmol/1
22.3 21.9 21.8
REW %
14.2 13.9 13.0
PLATELETS G/l
1198 1015 1030
ANIMAL NUMBER
25 26 27
HAEMATOLOGY PARAMETERS
WBC
SBS.
EO.
G/l 1
1
14.9 8.6
12.5
0.075 0.210
0.095
0.005 0.000
0.010
BASO. 1
0.000 0.000 0.000
MONO. 1
LYMPH. i
0.015
0.905
0.030
0.760
0.020 - 0.875
PT SEC
15.3 15.6 14.7
PTT SEC
15.0 16.1 14.8
1 99
NOTOX Project 242933
HAEMATOLOGY AFTER 2 WEEKS RECOVERY HALES GROUP 3 (TELQMER ACRYLATE)
BACK TO MAIN
H EM-IND - 15 3O-MAR-9 9
ANIMAL NUMBER
41 42 43
HAEMATOLOGY PARAMETERS
RBC HB
HCT
T/l
mmol/1
1/1
6.83 6.80 7.28
8.7 8.3 9.2
0.396 0.386 0.421
mcv fl
58.0 56.8 57.8
MCH fmol
1.274 1.221 1.264
M2HC mnol/1
22.0 21.5 21.9
RDW
13.8 13.8 12.5
. PLATELETS G/l
1239 1323
995
ANIMAL NUMBER
41 42 43
HAEMATOLOGY PARAMETERS
WBC
SEE.
BO.
G/l 1
1
1 2 .1 13.9 10.2
0.090 0.040 0.135
0.010 0.000
0.005
BASO. 1
0.000 0.000 0.000
MONO. 1
0.025 0.020 0.030
LYMPH. 1
0.875 0.940 0.830
PT SEC
15.3 14.1 15.2
PIT SEC
17.1 15.8 15.2
200
BACK TO MAIN
NOTOX Project 242933
-
HAEMATOLOGY AFTER 2 WEEKS RECOVERY MALES GROUP 4 ( )
H E M - I N D - 16 3 0 - M A R - 99
*
ANIMAL NUMBER
57 58 59
HAEMATOLOGY PARAMETERS
RBC HB
HOT
T/l
mrool/1
1/1
6.76 6.94 7.29
8.7 8.8
8.9
0.389 0.400 0.416
irvcv fl
57.5 57.6 57.1
MCH fmol
1.287 1.268 1.221
MCHC mmol/l
22.4 22.0 21.4
REW %
12.9 12.3 14.8
PLATELETS G/l
' 1099 1116 1024
ANIMAL NUMBER
57 58 59
HAEMATOLOGY PARAMETERS
WBC
SEG.
BO.
G/l 1
1
14.0 12.2 10.5
0.080 0.050 0.045
0.000 0.005 0.005
BASO. 1
0.000 0.000 0.000
MONO. 1
0.030 0.020 0.020
LYMPH. . FT 1 SEC
0.890 0.925 0.930
15.0 14.8 13.9
PTT SEC
16.2 15.3 13.6
201
BACK TO MAIN
NOTOX Project 242933
HAEMATOLOGY AFTER 2 WEEKS RECOVERY MALES GROUP 5 (PFHS)
HEM-IND - 17 3 0 - M A R - 99
ANIMAL
NUMBER
73 74 75
HAEMATOLOGY PARAMETERS
RBC
T /l
HB irm o l/1
HCT 1/1
6.86 6.84 7.21
8.5 8.5 8.7
0.388 0.395 0.394
m cv fl
56.6 57.7 54.6
MCH fm o l
1.239 1.243 1.207
MCHC
m m o l/l
21.9 21.5
2 2 .1
RDM
14.0 15.0 14.2
PLATELETS
G /l
1028 1071 1059
ANIMAL
NUMBER
73 74 75
HAEMATOLOGY PARAMETERS
NBC
SBG.
EO.
G/l 1
1
11.1 6.4 7.9
0.200
0.075 0.125
0.015 0.010 0.010
BASO. 1
0.000 0.000 0.000
MONO. 1
0.025 0.010 0.020
LYMPH. 1
0.760 0.905 0.845
PT SEC
15.5 14.9 --
FTT SEC
15.3 15.0 --
202
BACK TO MAIN
NOTOX Project 242933
HAEMATOLOGY AFTER 2 WEEKS RECOVERY MALES GROUP 6 (PFOS)
HEM-IND - 18 3 0 - M A R - 99
ANIMAL NUMBER
89 90 91 .
HAEMATOLOGY PARAMETERS
RBC HB
HOT
T/l
mnol/1
1/1
6.76 7.15 7.13
8.9 8.9 8.9
0.401 0.412 0.411
mcv fl
59.3 57.6 57.6
MCH fmol
1.317 1.245 1.248
MCHC imvol/1
22.2 21.6 21.7
ROT %
14.0 12.9 12.5
PLATELETS G/l
' 1068 1026 1140
ANIMAL NUMBER
89 90 91
HAEMATOLOGY PARAMETERS
WBC
SEG.
EO.
G/l 1
1
12.7 12.8
9.4
0.125 0.155 0.130
0.000
0.005 0.000
BASO. 1
0.000 0.000 0.000
MONO. 1
LYMPH. 1
0.020 0.020
0.015
' 0.855 0.820 0.855
FT SEC .
PIT SEC
15.6 ' 15.9 14.9
16.3 14.6 13.2
203
NOTOX Project 242933
HAEMATOLOGY AFTER 2 WEEKS RECOVERY FEMALES GROUP 1 (VEHICLE CONTROL)
BACK TO MAIN
HEM-IND - 19 3 O-MAR-99
ANIMAL NUMBER
105 106 107
HAEMATOLOGY PARAMETERS
RBC HB
HCT
T/l
itmol/1
1/1
7.02 7.11 6.93
8.8 9.2 8.9
0.399 0.414 0.398
mcv fl
56.8 58.2 57.4
MCH fmol
1.254 1.294 1.284
MCHC irmol/1
22.1 22.2 22.4
REM %
15.8 12.9 14.7
PLATELETS G/l 1410 1083 1204
ANIMAL
NUMBER
105 106 107
HAEMATOLOGY PARAMETERS
WBC
SEG.
EO.
G/l 1
1
9.2
0.055
0.000
7.1
0.115
0.010
5.7
0.055
0.005
BASO. 1
0.000 0.000 0.000
MONO. 1
LYMPH. .1
0.025 0.015 0.010
0.920 0.860 0.930
PT SEC
16.2 15.5 16.3
PTT SBC
17.7 14.7 14.0
204
NOTOX Project 242933
HAEMATOLOGY AFTER 2 WEEKS RECOVERY FEMALES GROUP 2 (TELOMER ALCOHOL)
BACK TO MAIN
HEM-IND - 20 3 0 - M A R - 99
ANIMAL NUMBER
121 122 123
HAEMATOLOGY PARAMETERS
RBC HB
HOT
T/l
irmol/1
1/1
6.26 6.63 6.52
8.6
8.4 8.3
0.378 0.382 0.373
mcv fl
60.4 57.6 57.2
MCH fmol
1.374 1.267 1.273
MCHC nmol/1
22.8 22.0 22.3
REW %
14.8 16.4 14.4
PLATELETS G/l 1283 1024 1045
ANIMAL NUMBER
121 122 123
HAEMATOLOGY PARAMETERS
WBC
SEG.
EO.
G/l 1 . 1
10.9 5.6 9.6
0.095 0.080 0.055
0.000 0.005 0.015
BASO. 1
0.000 0.000 0.000
MONO. 1
0.035 0.025 0.015
LYMPH. 1
0.870 0.890 0.915
PT SEC
14.6 14.9 15.3
FTT SEC
14.6 14.1 14.6
205
BACK TO MAIN
NOTOX Project 242933
-
HAEMATOLOGY AFTER 2 WEEKS RECOVERY
FEMALES GROUP 3 (TELOMER ACRYLATE)
H E M - I N D - 21 3 0 - M A R - 99
A N IM AL NUMBER
137 138 139
haematology parameters
RBC HB
HCT
T/l
mmol/I
1/1
6.49 6.88 6.49
8.9 8.7 8.4
0.402 0.387 0.379
m cv fl
61.9 56.3 58.4
MCH finol .
1.371 1.265 1.294
MCHC mnol/1
22.1 22.5 22.2
REW PLATELETS % G/l
15.9 13.9 16.3
1053 1205
939
A N IM AL NUMBER
HAEMATOLOGY PARAMETERS
WBC
SEG.
EO.
G/l 1
1
137 1 1 .0 138 6.0
139 4.8
0.060 0.050 0.085 .
0.005 0.005 0.000
baso :
1
0.000 0.000 0.000
MONO. 1
0.030 0.005 0.015
LYMPH. 1
PT SEC
PTT SEC
0.905 0.940 0.900
' 16.1 15.8 15.0
16.3 14.8 14.2
206
NOTOX Project 242933
HAEMATOLOGY AFTER 2 WEEKS RECOVERY FEMALES GROUP 4 ( )
BACK TO MAIN
H E M - I N D - 22 3 0 - M A R - 99
ANIMAL NUMBER
153 154 155
HAEMATOLOGY PARAMETERS
RBC HB
HOT
T/l
irmol/1
1/1
6.59 7.27 6.27
8.8 9.4 8.7 -
0.397 0.408 0.384
mcv fl
60.2 56.1 61.2
MCH frnol 1.335 1.293 1.388
MCHC nmol/l
22.2 23.0 22.7.
ROW 17.2 14.0 14.7
PLATELETS G/l
1060 1171 1102
ANIMAL NUMBER
153 154 155
HAEMATOLOGY PARAMETERS
WBC G/l
SEG. 1
EO. 1
7.9
0.075
0.005
6.9
0.050
0.015
5.3
0.115
0.010
BASO. 1
0.000 0.000 0.000
toro. 1
0.045 0.035 0.020
LYMPH. 1
0.875 0.900 0.855
PT PTT SEC SEC
14.8 ' 15.1 14.5
15.7 14.0 14.6
207
BACK TO MAIN
NOTOX Project 242933
HAEMATOLOGY AFTER 2 WEEKS RECOVERY FEMALES GROUP 5 (PFHS)
H E M - I N D - 23 30-MAR-9 9
ANIMAL NUMBER
169 170 171
haem atology param eters
RBC HB
HCT
T/l
irmol/1
1/1
6.22 6.70 6.91
8.2 8.4 9.2
0.357 0.376 0.416
mcv fl
57.4 56.1 60.2
MCH . MCHC fmol ' irmol/1
1.318 1.254 1.331
23.0 22.3 22.1
RDM %
14.0 15.8 13.9
PLATELETS G/l
1010 828 723
ANIMAL NUMBER
169 170 171
HAEMATOLOGY PARAMETERS
WBC
SEG.
EO.
G/l 1
1
5.9
0.085
0.005
7.7
0.035
0.020
6 .1
0.030
0.000
BASO. 1
0.000 0.000 0.000
MONO. 1
0.015 0.000 0.015
LYMPH. 1
0.895 0.945 0.955
PT SEC
PIT SEC
... 14.7 14.9
16.6 14.8
208
BACK TO MAIN
NOTOX Project 242933
HAEMATOLOGY
-
AFTER 2 WEEKS RECOVERY
6FEMALES
GROUP (PFOS)
H E M - I N D - 24 3 0-MAR-99
*
ANIMAL NUMBER
185 186 187
HAEMATOLOGY PARAMETERS
RBC HB
HOT
T/l
rrmol/1
1/1
6.48 6.23 6.88
8.5 0.382 8.2 . 0.374 8.9 0.405
mcv fl
59.0 60.0 58.9
MCH fevol 1.312 1.316 1.294
MCHC nmol/1
22.3 21.9 22.0
ROT %
15.8 15.8 15.6
platelets
G/l 1206
769 1127
ANIMAL NUMBER
185 186 187
HAEMATOLOGY PARAMETERS
WBC
SEG.
BO.
G/l 1
1
5.3
0.115
0.000
9.6
0.100
0.000
5.4
0.085
0.005
BASO. 1
0.000 0.000 0.000
MONO. 1
0.025 0.020 0.035
LYMPH. 1
0.860 0.880 0.875
PT PTT SEC SEC
15.4 ' 15.5
13.7 13.7
209
BACK TO MAIN
NOTOX Project 242933
_ -.....
HAEMATOLOGY AFTER 4 WEEKS RECOVERY MALES GROUP 1 (VEHICLE CONTROL)
H E M - I N D - 25 3O-MAR-9 9
*
ANIMAL NUMBER
13 14 15
HAEMATOLOGY PARAMETERS
RBC HB
HOT
T/l
ntnol/1
1/1
7.52 7.75 6.79
8.9 9.8 8.7
0.391 0.424 0.373
mcv fl
52.0 54.7 54.9
MCH fmol 1.184 1.265 1.281
MCHC rmvol/1
22.8 23.1 23.3
ROW %
14.4 14.1 14.9
PLATELETS G/l
1062 981
1204
ANIMAL NUMBER
13 14 15
HAEMATOLOGY PARAMETERS
WBC
SEG.
EO.
G/l 1
1
13.0 12.5 11.2
0.055 0.160 0.095
0.010 0.010 0.000
BASO. 1
0.000 0.000 0.000
MONO. 1
0.075 0.055 0.025
LYMPH. 1
0.860 0.775 0.880
PT SEC
12.7 -- 13.5
PIT SBC
16.1 -- 15.9
BACK TO MAIN
NOTOX Project 242933
_
HAEMATOLOGY AFTER 4 WEEKS RECOVERY HALES GROUP 2 (TELOMER ALCOHOL)
H E M - I N D - 26 3 0 - M A R - 99
*
ANIMAL NUMBER
29 30 31
HAEMATOLOGY PARAMETERS
RBC HB
HOT
T /l
mmol/1
1/1
7.16 6.88
6.98
9.1 9.2 8.9
0.396 0.390 0.386
mcv fl
55.3 56.7 55.3
MCH
fmol
1.271 1.337 1.275
MCHC
irmol/1
23.0 23.6 23.1
REW
%
12.7 12.6 13.5
PLATELETS
G/l
917 1097 1062
ANIMAL
NUMBER
29 30 31
HAEMATOLOGY PARAMETERS
WBC
SEG.
EO.
G/l 1
1
10.7 10.5 15.8
0.120 0.065 0.100
0.010 0.000 0.000
BASO. 1
0.000 0.000 0.000
MONO.
1
0.075 0.040 0.050
LYMPH.
1
0.795 0.895 0.850
PT SEC 13.3 12.7
PTT SEC
14.6 15.7
21 1
BACK TO MAIN
NOTOX Project 242933
-
HAEMATOLOGY AFTER 4 WEEKS RECOVERY MALES GROUP 3 (TELOMER ACRYLATE)
HEM-XND - 27 3 0 - M A R - 99
*
ANIMAL NUMBER
45 46 47
HAEMATOLOGY PARAMETERS
RBC HB
HOT
T/l
xnnol/1
1/1
6.84 6.70 7.01
8.9 8.9 9.4
0.372 0.386 0.395
mcv fl
54.4 57.6 56.3
MCH fool
1.301 1.328 1.341
MCHC ircnol/1
23.9 23.1 23.8
REW %
13.9 13.8 13.3
PLATELETS G/l
1244 960
1024
ANIMAL NUMBER
45 46 47
HAEMATOLOGY PARAMETERS
NBC
SEG.
EO.
G/l 1
1
15.6 8.6
8.2
0.085 0.125 0.060
0.005 0.015 0.010
BASO. 1
0.000 0.000 0.000
MONO. 1 0.025 0.085 0.030
LYMPH. 1 0.885 0.775 0.900
PT SEC 13.3 13.6 13.2
PTT SEC
16.7 16.6 17.0
21 2
BACK TO MAIN
NOTOX Project 242933
HAEMATOLOGY AFTER 4 WEEKS RECOVERY MALES GROUP 4 ( )
HEM-IND - 28 3 0 - M A R - 99
ANIMAL
NUMBER
61 62 63
HAEMATOLOGY PARAMETERS
RBC KB
HCT
T/l
nnol/1
1/1
6.84 7.18 7.01
9.2 9.5 9.0
0.379 0.408 0.388
xncv fi
55.4 56.8 55.3
MCH fznol 1.345 1.323 1.284
MCHC mnol/1 24.3 23.3 23.2
REW %
13.4 12.6 12.7
PLATELETS
G/l
1232 1206 1100
ANIMAL NUMBER
61 62 ' 63
HAEMATOLOGY PARAMETERS
WBC
SEG.
EO.
G/l 1
1
10.7 9.2 8.2
0.055 0.055 0.070
0.005 0.010
0.000
BASO. 1
0.000 0.000 0.000
MONO. 1
0.030 0.015 0.015
LYMPH. 1
0.910 0.920 0.915
PT SEC 13.0 14.6
PTT SEC 15.9 17.0
21 3
NOTOX Project 242933
HAEMATOLOGY AFTER 4 WEEKS RECOVERY MALES GROUP 5 (PFHS)
BACK TO MAIN
H EM-IND - 29 3 0 - M A R - 99
ANIM AL NUMBER
77 78 79
HAEMATOLOGY PARAMETERS
RBC HB
HOT
T/l
nmol/1
1/1
6.82 6.87 6.97
8.9 8.7 9.4
0.384 0.381 0.406
m cv 1
56.3 55.5 58.2
MCH ftnol 1.305 1.266 1.349
M0HC nmol/1
23.2 22.8 23.2
REM
t
13.2 13.8 13.8
PLATELETS G/l
1000 884
1013
ANIMAL
NUMBER
77 78 79
HAEMATOLOGY PARAMETERS
WBC
SEG.
EO.
G/l 1
1
9.5
0.060
0.005
7.1
0.065
0.010
6.5
0.065
0.005
BASO. 1
0.000 0.000 0.000
MONO. 1
0.020 0.035 0.020
LYMPH. 1
0.915 0.890 0.910
PT SEC
14.5 -- ' 13.8
PIT SEC
14.9 -- 15.5
214
NOTOX Project 242933
HAEMATOLOGY AFTER 4 WEEKS RECOVERY MALES GROUP 6 (PFOS)
BACK TO MAIN
H EM-IND - 30 3 0 - M A R -9 9
ANIMAL NUMBER
93 94 95
HAEMATOLOGY PARAMETERS
RBC HB
HOT
T/l
ninol/1
1/1
7.14 7.20 6.63
8.9 9.2 8.8
0.383 0.406 0.378
mcv fl
53.6 56.4 57.0
MCH fmol
1.246 1.278 1.327
MCHC imvol/1
23.2 22.7 23.3
REW %
14.7 13.2 13.2
PLATELETS G/l 1083 1047 1101
A N IM A L
NUMBER.
93 94 95
HAEMATOLOGY PARAMETERS
WBC
SEG.
EO.
G/l 1
1
7.5 12.7 13.5
0.040 0.065 0.130
0.005 0.010
0.005
BASO. 1
0.000 0.000 0.000
MONO. 1
0.030 0.005 0.045
LYMPH. 1 0.925 0.920 0.820
PT . SEC
PTT SEC
14.2 ' 13.9 13.8
13.2 16.9 13.7
21 5
NOTOX Project 242933
HAEMATOLOGY AFTER 4 WEEKS RECOVERY FEMALES GROUP 1 (VEHICLE CONTROL)
BACK TO MAIN
HEM-IND - 31 3 0 - M A R - 99
ANIMAL NUMBER
109 110 111
HAEMATOLOGY PARAMETERS
RBC HB
HOT
T/l
mmol/l
1/1
6.76 7.09 6.76
9.2 9.4 9.2
0.380 0.423 0.389
mcv fl
56.2 59.7 57.5
MCH fmol
1.361 1.326 1.361
MCHC rrmol/1
24.2 22.2 23.7
ROW %
12.2 13.7 12.5
PLATELETS G/l
911 992 1013
ANIMAL NUMBER
109 110 111
HAEMATOLOGY PARAMETERS
WBC
SEG.
BO.
G/l 1
1
5.9
0.020
0.000
6.7
0.080
0.005
5.5
0.060
0.020
BASO. 1
MONO. 1
0.000
0.000 0.000
. 0.020
0.015 0.010
LYMPH. 1
0.960 0.900 0.910
PT PTT SEC SBC
13.0 12.0
11.8
14.6 16.9 15.6
21 6
NOTOX Project 242933
HAEMATOLOGY AFTER 4 WEEKS RECOVERY FEMALES GROUP 2 (TELOMER ALCOHOL)
BACK TO MAIN
HEM-IND - 32 3 0 - M A R - 99
AN IM AL NUMBER
125 126 127
HAEMATOLOGY PARAMETERS
RBC
HB
HOT
T/l
nrnol/1
1/1
6.95 6.85 6.77
9.4 9.5 9.2
0.400 0.403 0.404
m cv
fl
57.6 58.8 59.7
MCH
fmol
1.353 1.387 1.359
MCHC
mtnol/1
23.5 23.6 22.8
ROW
%
13.1 14.0 13.4
platelets
G /l
1088 882 969
ANIMAL NUMBER
125 126 127
HAEMATOLOGY PARAMETERS
me
SBG.
EO.
G/l 1
1
8.7
0.055
0.005
9.9
0.075
0.005
7.8
0.080
0.000
BASO. 1
0.000 0.000 0.000
MONO. 1
0.035 0.025 0.020
LYMPH. 1
0.905 0.895 0.900
PT PTT SEC SEC
11.9 12.2 ' 12.5
14.1 14.7 15.0
21 7
NOTOX Project 242933
HAEMATOLOGY AFTER 4 WEEKS RECOVERY
I*1k M A T Rfi
GROUP 3 (TELOMER ACRYLATE)
BACK TO MAIN
H E M - I N D - 33 3 0 - M A R - 99
ANIMAL NUMBER
141 142 143
HAEMATOLOGY PARAMETERS
RBC HB
HOT
T/l
rrmol/1
1/1
6.87 7.01 6.48
8.9 9.2 8.8
0.381 0.398 0.388
racv fl
55.5 56.8 59.9
MCH fmol
1.295 1.312 1.358
tHC irmol/1
23.4 23.1 22.7
ROW
13.3 13.1 13.4
platelets
G/l
1093 841 961
ANIMAL NUMBER
141 142 143
HAEMATOLOGY PARAMETERS
WBC
SEG.
EO.
G/l 1
1
10.7 8.9 3.5
0.025 0.055 0.035
0.010 0.010 0.010
BASO. 1
0.000 0.000 0.000
MONO. 1
0.035 0.010 0.010
LYMPH. 1
0.930 0.925 0.945
PT SEC
12.2 11.9 11.5
PIT SEC
14.6 16.7 15.9
21 8
BACK TO MAIN
NOTOX Project 242933
HAEMATOLOGY
`
AFTER 4 WEEKS RECOVERY
FEMALES
GROUP 4 ( )
HEM-IND - 34 3 0 - M A R - 99
AN IM AL NUMBER
157 158 159
HAEMATOLOGY PARAMETERS
RBC HB
HOT
T/l
nmol/1
1/1
7.25 6.88 6.85
9.2 8.9 9.1
0.402 0.386 0.391
mcv fl
55.4 56.1 57.1
MCH fmol -
1.269 1.294 1.328
MCHC nrool/1
22.9 23.1 23.3
RDW
12.9 12.5 12.3
PLATELETS G/l
1028 1045
969
HAEMATOLOGY PARAMETERS
ANIMAL
WBC
NUMBER G/l
SEG. 1
DO. 1
157 6.3 158 6.5 159 5.8
0.025 0.095 0.110
0.020 0.020
0.020
BASO. 1
0.000 0.000 0.000
MONO. 1
0.030 0.030 0.030
LYMPH. 1
0.925 0.855 0.840
PT SEC
12.5 11.5 12.6
PTT SEC
14.6 14.6 15.7
21 9
NOTOX Project 242933
HAEMATOLOGY AFTER 4 WEEKS RECOVERY FEMALES ROUP 5 (PFHS)
BACK TO MAIN
HEM-IND - 35 3 0 - M A R - 99
ANIMAL NUMBER
173 174 175
HAEMATOLOGY' PARAMETERS
RBC HB
HCT
T/l
innol/1
1/1
7.08 6.73 6.59
9.0 9.2 8.6
0.388 0.411 0.368
ircv fl
54.8 61.1 55.8
MCH fmol
1.271 1.367 1.305
MCHC mmol/1
23.2 22.4 23.4
REW %
13.7 1 2 .1 14.1
PLATELETS G/l
939 874 1120
ANIMAL NUMBER
173 174 175
HAEMATOLOGY PARAMETERS
WBC
SEG.
EO.
G/l 1
1
6.9
0.095
0.010
4.5
0.065
0.005
9.1
0.065
0.005
BASO. 1
0.000 0.000 0.000
MONO. 1
0.040 0.050 0.015
LYMPH. 1
0.855 0.880 0.915
PT PIT SEC SEC
11.8 12.0 11.4
15.2 15.5 15.1
220
BACK TO MAIN
NOTOX Project 242933
HAEMATOLOGY AFTER 4 WEEKS RECOVERY FEMALES GROUP 6 (PFOS)
HEM-IND - 36 3 0 - M A R - 99
ANIMAL
NUMBER
189 190 191
haem atology param eters
RBC HB
HCT
T/l
irmol/1
1/1
6.49 6.73 6.45
8.7 9.2
8.1
0.370 0.413 0.355
m cv
fl
57.0 61.4 55.0
MCH frool
1.341 1.367 1.256
MCHC ninol/1
23.5 22.3 22.8
REM
%
11.9 13.3 13.5
PLATELETS G/l
1062 906
1013
ANIMAL NUMBER
189 190 191
HAEMATOLOGY PARAMETERS
WBC
SEG.
EO.
G/l 1
1
11.3 7.4 7.4
0.085 0.065 0.035
0.000 0.015 0.000
BASO.
1
0.000 0.000 0.000
MONO. .1
0.015 0.025 0.010
LYMPH. 1 0.900 0.895 0.955
PT SEC
12.0 12.7 12.0
PIT SEC
13.6 15.5 15.5
221
NOTOX Project 242933
CLINICAL BIOCHEMISTRY AFTER 4 WEEKS MALES GROUP 1 (VEHICLE CONTROL)
BACK TO MAIN
CHE-IND - 1 3 0 -M AR -99
ANIMAL NUMBER
1 2 3 4. 5 6 7 8 9 10 11 13 14 15
ALAT(GPT) ASAT(GOT) BILI T.
ukat/1
ukat/1
umol/1
CHQLEST.T. TRIGL.
mmol/1
ranol/1
0.88 0.69 0.79 0.60 0.66 0.51 0.67 0.50 0.58 0.70 0.56 0.54 0.53 0.80
3.21 2.55 2.34 3.28 3.30 2.77 2.76 2.16 2.42 2.33 3.13 2.92 2.86 3.10
3 1 2 1 3 3 3 2 3 3 2 2 2 3
1.58 1.94 2.63 2.36 1.47 2.14 2.07 1.70 2.47 2.53 1.53 2.04 1.85 1.64
,
0.75 0.80 0.63 1.27 0.54 0.83 1.12 0.59 0.53 0.88 0.61 1.05 0.52 1.07
CREATININE GLUCOSE UREA
umol/1
mnol/1
mnol/1
35
6.63
6.5
29
5.67
5.9
37
6.01
8.8
31
5.65
5.8
32
7.14
6.5
29
7.50
5.9
29
6.31
5.5
28
5.56
6.5
38
6.70
8.1
38
6.04
8.6
33
6.93
7.7
38
6.14
7.5
30
4.94
5.9
39
7.31
6.9
ANIMAL NUMBER
1 2 3 4 5 6 7 8 9 10 11 13 14 15
PROTEIN T. ALBUMIN GLOBULIN A/G RATIO ALP
g/1 g/1 g/1
ukat/1
54.4 56.6 57.5 57.8 59.9 59.1 62.5 57.5 55.2 50.8 53.5 55.9 57.6 56.6
29.0 29.6 30.4 30.4 30.4 28.9 30.2 28.5 28.5 27.7 30.0 29.2 28.6 29.2
25.4 27.0 27.1 27.4 29.5 30.2 32.3 29.0 26.7 23.1 23.5 26.7 29.0 27.4
1.1 1.1 1.1 1.1 1.0 1.0 0.9 1.0 1.1 1.2 1.3 1.1 1.0 1.1
12.54 7.29 6.03 7.45 8.56 6.65 6.99 8.54 6.11
10.02 7.26 7.58 7.29 6.26
INORG PHOS SODIUM
mnol/1
mnol/1
2.76 3.22 2.79 2.63 2.86 2.71 2.85 3.04 2.73 2.70 2.78 2.80 3.00 2.78
143.5 143.8 143.1 143.1 144.8 142.3 143.3 142.8 141.6 143.1 143.3 141.3 139.8 140.6
POTASSIUM mmol/1 4.70 5.32 4.50 4.72 4.50 4.72 4.70 4.69 5.10 4.70 5.07 4.67 5.56 4.96
ANIMAL NUMBER
CALCIUM mnol/1
CHLORIDE mnol/1
1 2.46 104
2 2.68 101
3 2.53 101
4 2.55 102
5 2.63
98
6 2.58
97
7 2.64
99
8 2.48 100
9 2.47 100
10 2.43 100
11 2.47
99
13 2.49 102
14 2.58
95
15 2.49
97
222
NOTOX Project 242933
-
CLINICAL BIOCHEMISTRY AFTER 4 WEEKS MALES GROUP 2 (TELOMER ALCOHOL)
BACK TO MAIN
CHE-IND - 2 3 0 - M A R - 99
*
ANIMAL NUMBER
17 18 19 20 21 22 23 24 25 26 27 29 30 31
ALAT(GFT) ASAT(GOT) BILI T.
ukat/1
ukat/1
umol/1
CHOLEST.T. TRIGL.
rrtnol/1
nmol/1
0.56 0.47 0.71
0.83 0.52 0.62 0.70 0.65 0.56 0.48 0.64
0.51 0.60 0.66
1.85 2.26 2.48 3.02 2.20
2.63 2.63 2.38 2.46 2.64 2.96 2.68 3.14 3.41
2 2
2 2 2
2 2
2
2 3 3 3 2
2
1.37 1.67 1.34 0.99 1.0 1
1.11 0.86
1.02
2.58 1.14 1.31 1.48 1.13 0.92
0.87
1.23 1.20
0.30 0.37
0.80 0.47 0.39 0.54 0.43 0.56 0.56 0.60
0.49
CREATININE GLUCOSE
umol/1
nmol/1
urea' nmol/1
29 31 31 34 26 29 31 30 32 30 34 29 29
24
6.37 6.47 7.16 5.68 8.71 7.19 6.58 6.85 6.17 4.50 6.68
6.33 5.58
6.49
6.2
6.5 7.4 8.1 6.7 6 .1 6 .1
8.3 6.7 8.3 8.3 5.5 5.9 7.2 .
ANIMAL NUMBER
17 18 19 20 21 22 23 24 25 26 27 29 30 31
PROTEIN T. ALBUMIN g/i g/i
GLOBULIN A/G RATIO ALP g/i ukat/1
53.0 53.8 54.3 54.9 54.2 53.0 52.6 52.6
53.1 53.1 50.5 58.0 51.9 49.1
28.0 29.3 31.5 30.8 27.3 28.8 27.6 29.5
27.8 28.2 27.7 29.3 29.2 27.4
25.0 24.5 22.8
24.1 26.9 24.2 25.0 23.1 25.3 24.9 22.8
28.7 22.7 21.7
1 . 1 6.46 1 .2 8.92 1.4 7.59 1.3 6.81 1.0 8.12 1 .2 7.04 1 . 1 6.03 1.3 6.84 1 .1 . 8.21 1 . 1 11.53 1 .2 7.94 1 .0 9.19 1.3 12.49 1.3 6.01
INORO PHDS SODIUM
mnol/1
mrol/1
2.60 3.04 2.93
2.69 2.64 2.90 2.69 2.76 2.65 2.71 2.72 2.80 2.61 2.76
143.9 143.3 143.1 143.3 143.4 142.6 142.1 141.4
142.3 143.5 141.8 144.8 142.5 141.9
POTASSIUM nmol/1
4.25 4.65 4.40 4.55 5.07 4.64 4.49 5.14 4.60 4.34 4.78 4.69 4.24 4.51
ANIMAL NUMBER
CALCIUM nmol/1
CHLORIDE mmol/1
17
18 19 20 21
22
23 24 25 26 27 `
29 30 31
2.58 2.48 2.55
2.50 2.49 2.47
2.43 2.44 2.42 2.42 2.37
2.59 2.43 2.44
101
100 102
103 94 99
101
99 101
102 101
100 101 100
223
NOTOX Project 242933
CLINICAL BIOCHEMISTRY AFTER 4 WEEKS MALES GROUP 3 (TELOMER ACRYLATE)
BACK TO MAIN
CHE-IND - 3 3 0 - M A R - 99
ANIMAL NUMBER
33 34 35 36 37 38 39 40 41 42 43 45 46 47
ALAT(GPT) ASAT(GOT) BILI T.
ukat/1
ukat/1
umol/1
CHOLEST.T. TRIGL.
nmol/1
nmol/1
0.58 0.85 0.64 0.62 0.52 0.45 0.81 0.44 0.48 0.50 0.73 0.72
--
0.57
3.74 2.81 2.72 2.46 2.00
2.31 2.85 1.87 2.46 2.27 2.62 2.98 2.48 2.52
2 2 1
2 2
2 3 5 2
2 .2
3 2
2
1.23 2.72 1.19 0.89 1.13 1.91 1.13 1.10 1.64 1.40 1.51 1.72 1.02
1.33
1.72 0.71
0.62 0.44 0.66
1.34 0.46 0.68
1.13 0.56 0.43 0.51 0.43 0.57
CREATININE GLUCOSE
umol/1
nmol/1
UREA nmol/1
31
6.30
5.7
29
5.63
6.0
34
6.80
7.2
33
5.77
7.2
28
6.56
5.7
29
6.66
7.5
30
6.95
6.2
32
7.48
8.5
31
7.21
5.7
31
6.26
8.1
30
5.24
6.2
34
5.48
7.7
31
5.86
6.5
27
5.22
4.9
AN IM AL NUMBER
PROTEIN T. ALBUMIN
. g/1
g /1
GLOBULIN A/G RATIO ALP g/i ukat/1
33 57.8 33.4 24.4 1.4
34 58.1 30.0 28.1 1 . 1
35 51.9 29.6 22.3 1.3
36 52.6 29.5 23.1 1.3
37 51.9 30.2 21.7 1.4
38 56.0 29.9 26.1 1 . 1
39 54.2 28.4 25.8 1 . 1
40 55.2 29.0 26.2 1 . 1
41 55.4 29.2 26.2 1 . 1
42 50.9 26.9 24.0 1 . 1
43 54.0 29.5 24.5 1 .2
45 52.7 29.2 23.5 1 .2
46
47.6
25.8
21.8
1.2
47 56.3 29.4 26.9 1 . 1
8.10
7.91 8.31 8.85 7.99 6.99 5.23 8.66
7.93 6.91
6.98 6.06 9.35 8.53
INORG PHDS SODIUM
nmol/1
nmol/1
2.88 2.95 2.80 3.10 3.04 2.69 2.90 3.22 2.92 2.79 2.55 2.90 3.01
2.61
143.7 143.5 143.2 144.5
143.1 144.0 142.6 143.7 143.6 143.0 142.4 141.7 143.0 142.6
POTASSIUM nmol/1
4.81 3.92 4.81 4.32 4.95 4.85 4.66 5.14 5.30 4.69 4.58 4.81 5.13 4.09
.
ANIMAL
NUMBER
33 34 35 36 37 38 39 40 41 42 43 45 46 47
CALCIUM nrool/1
CHLORIDE nmol/1
2.57
2.53 2.38 2.62 2.48 2.55 2.48 2.57
2.54 2.44 2.47
2.50 2.44 2.46
101
100 103 102 102
100 100 102
104 104 102 101
103 101
224
NOTOX Project 242933
CLINICAL BIOCHEMISTRY AFTER 4 WEEKS . MALES GROUP 4 ( )
BACK TO MAIN
CHE-IND - 4 30-MAR-99
ANIMAL NUMBER
49 50 51 52 53 54 55 56 57 58 59 61 62 63
ALAT(GPT) ASAT(GOT) BILI T.
ukat/1
iikat/1
umol/1
0.68 0.47 0.63 0.52 0.69 0.53 0.73 0.62 0.67 0.49
--
0.45 0.38 0.52
2.49 2.41 2.09
2.30 2.85 2.16 2.43
2.19 2.48 2.58 2.52 2.28 1.67 . . 1.85
2 2 2
3 2 2 2
3 3 2
3 2 2 2
CHOLEST.T. TRIGL.
imiol/1
mmol/l
1.62 1.73 1.75 1.76 1.65 1.39 1.33
2.56 2.37
2.23 1.54 1.15 1.98 1.51
0.56 0.58 1.22
0.85 0.51 0.50 0.73 0.84 0.66
0.75 0.60 0.45 0.50 0.40
CREATININE GLUCOSE
umol/1
mnsl/l
UREA mmol/l
35 34 29 31
29 37 34 32
42 32 35 38 40 . 31
7.70 6.58 8.07 7.05 5.86 7.41
7.04 7.39 7.38 5.75 6.29 6.57 4.62 4.58
7.8 6.7 .
6.4 6.8 5.7 5.6 7.1 6.9 8.6
6.8 6.2 6.6
9.0 6.2
ANIMAL NUMBER
49 50 51 52 53 54 55 56 57 58 59 61 62 63
PROTEIN T. ALBUMIN g/i g/x
GLOBULIN A/G RATIO ALP g/x ukat/1
57.3
51.9 57.0 56.7 54.2 52.8 53.5 53.2 51.0 56.4 53.2 53.3 51.8 50.7
.
31.0 30.2 31.0
32.5 28.8 28.9 30.4 28.6 29.3 31.1 28.6 30.5 28.3 ' 28.2
26.3 21.7 26.0
24.2 25.4
23.9 23.1 24.6 21.7 25.3 . 24.6 22.8
23.5 22.5
1.2
1.4 1.2
1.3 1.1
1.2 1.3 1.2 1.4 1.2 1.2
1.3 1.2 1.3
8.01
7.08 8.24 10.97 7.10 9.03 8.06 8.42 8.47
7.39 6.63 6.76 7.03 8.81
INORG PHDS SODIUM
mnol/1
mmol/l
2.56 2.84 2.67
2.59 2.83 2.72 2.54 2.72
3.19 2.40 2.58 2.76 3.15 2.45
143.8
143.1 141.3 142.6 143.2 143.4 142.4 143.0 142.5 142.7 143.3 143.0 143.8 144.3
POTASSIUM irmol/l
4.67 4.81 4.46 4.51 4.70 4.60 4.31 4.65 4.50 4.21 4.15 4.33 4.85 4.29
ANIMAL NUMBER
CALCIUM mmol/l
CHLORIDE irmol/1
49 2.45 101
50 2.43 104
51 2.52 100
52 2.55
98
53 2.48 102
54 2.41 102
55 2.45 102
56 2.36 101
57 2.54 103
58 2.57 101
59 2.37
99
61 2.47
95
62 2.56 104
63 2.45 102
225
NOTOX Project 242933
CLINICAL BIOCHEMISTRY AFTER 4 WEEKS MALES GROUP 5 (PFHS)
BACK TO MAIN
CHE-IND - 5 3 0 - M A R - 99
ANIMAL NUMBER
65 66 67 68 69 70 71 72 73 74 75 77 78 79
ALAT(GPT) ASAT(GOT) BILI T.
ukat/1
ukat/1
ianol/1
CHDLEST.T. TRIGL.
irmol/1
rnmol/1
0.56 0.68
0.59 0.63 0.50 0.57 0.60 0.66
0.61 0.65 0.59 0.56 1.53 0.64
1.95 2.21
2.08 2.30 2.69 2.44 2.73 2.57 2.42 2.43 2.01
2.27 2.74
2.60
2
1 2
1 2
2 2
2 2
3 2 2 2
2
1.22
1.20 1.12 0.91 0.87 0.83 0.83 1.15 0.78
1.19 1.23 . 0.92
0.70 0.68
0.83
0.46 0.59 0.37
0.29 0.30 0.42 0.51 0.33 0.51 0.40 0.36 0.23 0.37
CREATININE GLUCOSE
umol/1
mnol/1
UREA mmol/1
32
9.60
7.7
31
7.63
7.0
30
6.33
7.9
32
7.08
7.5
37
7.01
8.4
32
8.35
6.2
36
6.43
5.9
34
7.18
9.0
34
5.22
6.9
36
6.65
8.2
35
7.43
7.3
42
4.36
8.3
50
4.03
11.8
32
6.75
6.0
ANIMAL NUMBER
65 66 67 68 69 70 71 72 73 74 75 77 78 79
PROTEIN T. ALBUMIN g/1 g/1
GLOBULIN A/G RATTO ALP g/1 ukat/1
56.0 55.2 51.6 50.1
54.0 52.3 58.3 59.1
51.0 53.0 55.3 54.0 50.4
51.1
28.2 31.1 31.2 29.9
30.1 29.4 32.9 32.5 30.4 29.7 31.5 28.6 29.8 29.7
27.8
24.1 20.4 20.2
23.9 22.9 25.4 26.6 20.6 23.3 23.8 25.4 20.6 21.4
1.0
1.3 1.5 1.5 1.3 1.3 1.3 1.2
1.5 1.3 1.3 1.1 1.4 1.4
7.03 10.00
8.63 8.60 9.07 10.97 10.10
7.64 8.94 9.14 11.09 9.33 7.62
12.50
INORG PHQS SODIUM
imnol/ 1
irmol/1
3.16 2.75 3.05 2.65 3.00 3.05 3.74 2.85
3.15 3.23 2.88
2.49 3.11 3.12
143.1 143.1 143.9 143.1 144.3 142.9 144.6 142.2 144.5 143.0 143.4 142.4 142.1 143.7
POTASSIUM nttiol/ 1
4.44 4.04 4.56 4.23 4.48 4.62 5.64 4.35 4.17 5.41 4.39 4.24 4.30 4.78
ANIMAL NUMBER
65 66 67 68 69 70 71 72 73 74 75 77 78 79
CALCIUM CHLORIDE
irmol/1
mnol/1
2.66
2.45 2.56 2.40 2.54 2.51 2.64 2.53 2.54 2.56 2.44 2.40 2.49 2.48
100
101 103 101 105 102 105 104 104 102 98 103 103 106
226
NOTOX Project 242933
CLINICAL BIOCHEMISTRY AFTER 4 WEEKS MALES GROUP 6 (PFOS)
BACK TO MAIN
CHE-IND - 6 30-MAR-99
ANIMAL
NUMBER
81 82 83 84 85 86 87 88 89 . 90 91 93 94 95
ALAT(GPT) ASAT(GOT) BILI T.
ukat/1
ukat/1
umol/1
CHOLEST.T. TRIGL.
nmol/1
irmol/1
0.60 0.58 0.49 0.67
0.49 0.62 0.60 0.54 0.62 0.66
0.56 0.62 0.65 0.61
1.88
2.80 1.70
2.05 1.67 2.88
2.06 2.61 2.78 2.28 3.05 2.40 2.34 2.68
3* 1 2 2
3 3 2
3 3 3 3 2 2 2
1.99 1.09 0.81 1 .1 1 1.77 0.88
1.13 0.96 1.16 1.49 1.19 0.40 0.88 0.91
0.47 0.91 0.34
0.37 0.45 0.52 0.48 0.48 0.43 0.41 0.79 0.27 0.39 0.42
CREATININE GLUCOSE
umol/1
mraol/1
UREA nmol/1
30
5.84
6.5
28
7.19
6.5
38
8.85
8.8
30
5.70
7.1
29
4.92
7.0
30
8.51
6 .1
37
6.72
10.7
30
4.50
8.1
33
6.45
7.9
29
6.00
7.4
37
5.13
9.0
30
6.20
7.7
28
6.92
6.9
32
6.35
6.9
ANIMAL NUMBER
81 82 83 84 85 86 87 88 89 90 91 93 94 95
PROTEIN T. ALBUMIN g /1 g/i
GLOBULIN A/G RATIO ALP 3/1 ukat/1
53.0 54.9 54.9
55.9 53.1 54.7 57.5 51.6 53.0 53.7 56.4 49.7 53.6 55.0
31.2
30.3 31.4 29.5 30.1 29.2 29.4 28.2 28.5 28.4 27.7 28.0
28.1 29.7
21.8
1.4
24.6
1.2
23.5
1.3
26.4
1.1
23.0
1.3
25.5
1.1
28.1
1.0
23.4 ' 1 .2
24.5
1.2
25.3
1 .1
28.7
1.0
21.7
1.3
25.5
1 .1
25.3
1.2
5.41 8.58 9.87 4.56
5.55 8.46 7.00 7.24 7.98 6.12
8.68 8.14 7.23 6.78
INORG PHOS SODIUM
umol/1
nmol/1
POTASSIUM nmol/1
2.60 2.95 3.11 2.95 2.91 2.80 3.07 2.83 3.01 3.37
3.16 2.67 2.94 3.03
143.4
143.9 143.1 142.7 144.6 143.3
143.3 143.2 143.2 142.0 141.8 141.9 142.5 141.6
.
4.39 4.99 5.27 5.00 3.98 4.55 4.39 4.67 4.46 4.75 4.59 4.72 5.15 4.34
ANIMAL NUMBER
81 82 83 84 85 86 87 88 89 90 91 93 94 95
CALCIUM CHLORIDE nrool/1 nmol/1
2.59 2.47
2.58 2.52 2.58 2.66 2.57 2.44
2.50 2.57 2.52
2.35 2.53 2.45
101 100 102
106 105
101 106 102
104 106 100
103 106
99
227
NOTOX Project 242933
CLINICAL BIOCHEMISTRY AFTER 4 WEEKS FEMALES GROUP 1 (VEHICLE CONTROL)
BACK TO MAIN
CHE-IND - 7 3 0 - M A R - 99
ANIMAL NUMBER
97 98 99 100 101 102 103 104 105 106 107 109 110 111
ALAT(GPT) ASAT(GOT) BILI T.
ukat/1
ukat/1
unvol/1
CHDLEST.T. TRIGL.
mnol/1
mnol/1
0.69 0.39 0.44 0.69 0.61 0.53 0.47 0.52 0.65 0.40 0.46 0.33 0.64
0.46
2.74 1.94 2.47 3.09 2.94 2.97 2.75 2.70 2.39 2.35 2.86
2.26 2.93 2.22
4 3 2 2 4 3 3 2 2
3 2 3 2
3
2.74 1.68
2.44 1.81 1.97 1.87 1.75 2.44 1.68
2.86 1.92 2.45 2.47 2.81
0.81 0.33 0.71 0.40 0.80 0.51 0.44 0.62 0.48 0.88 0.37 0.68
0.69 0.74
CREATININE GLUCOSE
unol/1
mnol/1
UREA mnol/1
39 37 35 . 40 42 41 38 46 38 39 44 ' 42 40 40
5.58 5.92 5.98 6.07 5.96 5.69
5.65 6.20 5.66 7.58 6.41 5.67 6.42 5.95
1 0 .1 6 .1 6.2 6.4
11.0 11.6
7.1 8.6 9.4 8.8
8.3 7.9 6.7 8.6
ANIMAL
NUMBER
PROTEIN T. ALBUMIN g/1 g/1
GLOBULIN A/G RATIO ALP g/1 ukat/1
97 63.1 98 67.9
99 . 61.3 100 67.4 101 67.1 102 61.5 103 70.1 104 70.9 105 67.3 106 65.5 107 64.8 109 60.8 n o 69.8 1 1 1 64.7
32.0
35.1 32.2 34.1 34.4 33.4 36.4
34.9 34.0 34.6 32.6 32.1 36.1 34.2
31.1 32.8 29.1 33.3 32.7
28.1 33.7
36.0 33.3 30.9 32.2 28.7 33.7
30.5
1.0 1.1 1.1 1.0 1.1
1.2 1 .1
1.0 1.0 1.1 1.0 1 .1 1.1
1 .1
3.33 4.34 5.17 5.53 4.09 4.41 3.91 5.50
4.21 3.87
4.50 4.79 4.35 4.39
INORO PHOS SODIUM
mnol/1
mnol/1
POTASSIUM mnol/1
2.87 2.50 2.67. 2.61 2.68
2.93 2.27 2.52 2.87 2.68
2.50 2.12 2.37 2.49
142.4 144.1 146.1 139.8 143.7 142.1 141.6 141.7 141.0 141.4 142.0 141.4 142.7
141.1
4.85 3.41 3.96 4.59 4.03 4.78 4.08 4.18 4.13 4.09 3.77 3.91 3.87
4.21
ANIMAL NUMBER
97 98 99 100 101 102 103 104 105 106 107 109 110 111
CALCIUM mnol/1
CHLORIDE mnol/1
2.72 2.60 2.59 2.47 2.66
2.52 2.59
2.58 2.59 2.66
2.45 2.37 2.52 2.58
103 101
100
98 93 98 98 96 93 94 96 97 96 96
228
NOTOX Project 242933
CLINICAL BIOCHEMISTRY AFTER 4 WEEKS FEMALES GROUP 2 (TELOMER ALCOHOL)
BACK TO MAIN
CHE-IND - 8 30-MAR-9 9
ANIMAL NUMBER
113 114 115 116 117 118 119 120 121 122 123 125 126 127
ALAT(GPT) ASAT(GOT) BILI T.
ukat/1
ukat/1
umol/1
0.60 0.48 0.56 0.43 0.39 0.47 0.66
0.35 0.51 0.38 0.39 0.37 0.45 0.47
1.59 2.06
2.28 2.22 1.98 3.45 3.15 2.12
2.34 2.91 2.15 2.76 2.40 1.75
3 3 3 3 2
3 3 2
2
2 2 2
2 2
CHOLEST.T. TRIGL.
mmol/1
nriol/1
2.41 2.66 2.22
2.56 1.70 2.14 2.86
2.14 2.24 2.15 1.46 2.57 1.85 2.69
0.47 0.52 1.07
0.48 0.47 0.60
0.69 0.46
0.52 0.60 0.42 0.63 0.31 0.69
CREATININE GLUCOSE
umol/1
mtiol/1
UREA imtol/1
33
5.30
7.4
34
5.86
7.1
36
5.63
6.9
44
6.82
10 .1
35
6.08
7.2
42
6.00
11.7
40
5.66
9.1
36
5.90
8.6
41
7.40
9.5
39
5.79
7.4
36
5.70
6.3
37
5.76
8.5
39
5.38
8.6
44
5.29
8.7
ANIMAL NUMBER
113 114 115 116 117 118 119
120 121 122
123 125 126 127
PROTEIN T . ALBUMIN
g/i g/i
GLOBULIN
g/1
A/G RATIO ALP u k a t/ 1
6 6 .6
6 6 .1
6 8.2
65.6 61.3 67.0 67.9 63.6 59.6
6 8 .8
64.6 63.3 63.2 61.3
34.2 30.8 34.1 34.0
32.5 35.0
33.7 33.6 32.5 37.0 34.6 34.2 33.9 32.9
32.4 35.3 34.1 31.6 28.8 32.0 34.2 30.0 27.1 31.8 30.0 29.1
29.3 28.4
1 .1
0.9 1.0
1 .1 1 .1
1 .1
1.0
1 .1 1 .2
1 .2 1 .2 1 .2
1 .2
1 .2
2.57 4.21 3.54 3.67
4.13 3.19 5.23 4.08 5.43 5.30 3.19 2.78 4.25 5.05
INORG PHOS SODIUM
n tn o l/1
m m ol/1
2.57 2.69 2.43 2.63 2.28 2.70 2.69 2.71 2.60 2.39 2.48 2.57 2.41
2 .2 0
141.5 142.5
143.3 143.1 143.0 142.2 140.9 142.5 143.2 143.5 142.0 142.4 142.7 143.7
POTASSIUM B inol/1
4.20 4.43 4.08 3.91 3.79 4.79 4.55 4.14 4.44 4.19 3.79 4.04 4.10 3.49
ANIMAL NUMBER
113 114 115 116 117 118 119 120 121 122 123 125 126 127
CALCIUM mnol/1
CHLORIDE ramol/1
2.66
2.60 2.67 2.63 2.50 2.57
2.63 2.60 2.57 2.65 2.57 2.65 2.54
2.51
102
99 102
99 100 101
97 96 96 98 96 98 97
99
229
NOTOX Project 242933
_
CLINICAL BIOCHEMISTRY AFTER 4 WEEKS
FEMALES GROUP 3 (TELOMER ACRYLATE)
BACK TO MAIN
CHE-IND - 9 30-MAR-99
*
ANIMAL
NUMBER
129 130 131 132 133 134 135 136 137 138 139 141 142 143
ALAT(GPT) ASAT(GOT) BILI T.
ukat/1
ukat/1
unl/l
CHOLEST.T. TRIGL.
irmol/1
runol/1
0.30 0.57 0.47
0.49 0.84 0.76 0.66
0.60 0.43 0.44 0.47 0.55 0.58
0.51
1.66
2.18 2.08 1.97 2.32 2.15 2.60 2.45 1.99 2.82 1.73 2.47 2.12
2.82
2
3 2
2 3 3 3 3 2 2
3 3 3 3
1.74 1.83 1.76
2.50 2.88
2.53 2.17 1.68 1.41 2.03 1.99 2.14
2.18 2.39
0.51 0.49 0.67
0.56 0.74 0.81 0.80 0.62 0.41 0.60 0.53 0.38 0.40 0.59
CREATININE GLUCOSE
umol/1
umol/1
UREA
nnvol/1
32
5.27
5.5
37
5.23
8.4
34
6.67
7.0
37
5.78
9.4
37
6.32
10.4
38
5.83
9.7
44
5.62
9.5
38
6.25
8.5
45
5.83
11.4
40
6.89
8.4
40
6.20
7.3
41
6.08
11.3
38
5.98
7.0
43
7.96
9.6
ANIMAL NUMBER
129 130 131 132 ' 133 134 135 136 137 138 139 141 142 143
PROTEIN T. ALBUMIN g/1 g/1
GLOBULIN A/G RATIO ALP g/1 ukat/1
64.4 66.2 68.0
62.0 69.5 62.8 65.6 66.7 60.2 65.8
64.2 66.9 66.4 66.8
32.3 34.0 35.3
33.5 34.8 32.0 33.5
34.5 31.2 35.0 36.2 33.8 35.5 36.4
32.1 32.2 32.7
28.5 34.7 30.8 32.1
32.2 29.0
30.8 28.0 - 33.1 30.9 30.4
1.0
1 .1 1.1
1.2 1.0 1.0
. 1.0 1.1 1 .1
1 .1 1.3 1.0
1.1 1.2
3.01 4.48 3.02
5.49 3.20 5.05 5.59 3.32 5.91 5.72 3.94 6.27 3.43
4.90
INORG PHOS SODIUM
irmol/1
umol/1
2.43 2.58 2.40 2.73 2.41 2.72 2.69 2.38 2.29 2.45 2.14 2.64
2.55 2.10
143.8 143.2 143.1 142.7
142.8 140.9 140.9 143.4 142.6 144.3 143.0 142.0 143.1
140.8
POTASSIUM smol/1
3.81 4.28 3.64 3.97 3.89 4.31 4.41 4.04 4.44 3.63 4.01 4.59 4.02 4.56
ANIMAL NUMBER
129 130 131 132 133 134 135 136 137 138 139 141 142 143
CALCIUM mmol/1
CHLORIDE runol/1
2.60 2.60 2.54 2.57 2.72
2.55 2.64 2.61 2.52 2.60 2.65 2.63 2.64 2.54
99
99 100
99 98 96 96 96 100 98 96 102 95 100
230
NOTOX Project 242933
CLINICAL BIOCHEMISTRY AFTER 4 WEEKS FEMALES GROUP 4 ( )
BACK TO MAIN
C H E - I N D - 10 3 0 - M A R - 99
ANIMAL NUMBER
145 146 147 148 149 150 151 152 153 154 155 157 158 159
ALAT(GPT) ASAT(GOT) BILI T.
ukat/1
ukat/1
umol/1
CHOLEST.T. TRIGL.
nmol/1
nmol/1
0.41 0.26
0.49 0.57 0.44
0.61 0.56 0.55 0.49 0.47 0.54 0.49 0.68
0.74
2.21 2.15 2.27 1.80 1.74 2.67
2.34 2.21 2.67 2.18 2.64 2.99 1.97
2.31
2 3
3 2 3 5 4 4 2 2 2
3 3 3
1.73 2.25 2.61 2.38 2.20
2.70 2.80 2.08 ' 2.04 2.55 2.14
2.83 2.05 2.09
0.65 0.53 0.46 0.56 0.73 0.47
0.87 0.44 0.56 0.56 0.42 0.45 0.48 0.62
CREATININE GLUCOSE
umol/1
nmol/1
UREA nmol/1
37
5.98
6.7
42
6.02
8.2
41
5.37
8.5
34
5.79
7.1
36
6.92
9.8
42
5.40
10.6
43
6.00
9.9
45
5.98
10.6
43
5.78
8.9
37
6.69
5.5
43
6.21
10.9
44
5.68
9.2
40
5.39
8.4
46
6.74
11.3
ANIMAL NUMBER
PROTEIN T. ALBUMIN g/1 g/1
GLOBULIN A/G RATIO ALP g/1 ukat/1
145 67.8 146 67.5 147 . 64.8 148 61.7 149 67.7 150 67.2 151 65.3 152 70.3 153 70.7 154 60.9 155 65.0 157 67.6 158 67.0 159 63.3
35.0 33.0
30.1 32.0 34.5 34.1 34.7 37.6 38.3 32.9 32.4 32.7 33.6 32.8
32.8
34.5 34.7 29.7 33.2
33.1 30.6 32.7 32.4 28.0 32.6 34.9 33.4 30.5
1.1 1.0 0.9 1.1
1.0 1.0 1.1 1 .1 1.2
1.2 1.0 0.9 1.0 1 .1
3.53 5.95
4.82 4.31 3.45 3.68 3.07 3.66 3.72 5.74 4.54 4.17 5.22 4.36
INORG PHOS SODIUM
nmol/1
nmol/1
2.18 2.34 2.30 2.46 2.60 2.61 2.90 2.29 2.25 2.33 2.47 2.28 2.50 2.30
143.7 143.0
141.9 140.4 142.7
142.0 141.6 143.4
141.9 141.9 143.2 140.6 141.5 140.8
POTASSIUM nmol/1
3.82 4.06 4.45 3.91 4.47 4.31 4.28 3.82 4.30 4.24 4.46 4.37 3.93 4.30
ANIMAL NUMBER
145 146 147 148 149 150 151 152 153 154 155 157 158 159
CALCIUM nmol/1
CHLORIDE nmol/1
2.57 2.63 2.52 2.58 2.64 2.63 2.66 2.64
2.60 2.57 2.55 2.58 2.68
2.50
100
103 99 98 95 98
. 98 97
101 99
102 100 100
99
231
NOTOX Project 242933
CLINICAL BIOCHEMISTRY AFTER 4 WEEKS FEMALES GROUP 5 (PFHS)
BACK TO MAIN
CHE-IND - 11 3 0 - M A R - 99
ANIMAL NUMBER
161 162 163 164 165 166 167 168 169 170 171 173 174 175
ALAT(GPT) ASAT(GOT) .BILI T.
ukat/1
ukat/1
umol/1
CHOLEST.T. TRIGL.
itmol/1
umol/1
0.52 0.50 0.78 0.53 0.57
0.49 0.57 0.62 0.65 0.59 0.60 0.40 0.62 0.53
1.77 3.54 2.18 1.61 2.01 3.77 2.27 2.86 2.49 1.75 1.61 2.79 2.88
2.73
3 3 2
3 3 2
3 2 2
2 2
2 2
3
1.93 2.16 1.64 1.80 2.23 1.76 1.46 1.94 1.59 2.06 2.16 1.32 1.66
1.68
1.00
0.52 0.48 0.32 0.55 0.45 0.49 0.55 0.59 0.44 0.44 0.38 0.33 0.53
CREATININE GLUCOSE
umol/1
irmol/1
UREA itmol/1
38
6.34
7.7
40 . 5.01
7.2
38
4.93
9.6
35
6.21
7.9
35
5.64
7.8
37
5.33
7.6
39
6.02
9.6
45
5.96
9.1
44
5.77
8.5
40
5.92
8.2
42
7.44
7.8
44
6.18
8.6
44
6.23
9.2
42
7.30
9.9
ANIMAL
NUMBER
161 162 163 164 165 166 167 168 169 170 171 173 174 175
PROTEIN T. ALBUMIN g/l g/l
GLOBULIN A/G RATIO ALP g/l ukat/1
74.6 66.1
71.1
66.5 63.9 60.5 63.1 66.2
63.2 62.4 70.0 64.5 60.1 63.5
35.6 32.4 35.0 34.7 33.3 31.3 33.8 33.9 32.2 33.2
35.8 33.8 31.3 35.4
39.0 33.7 36.1 31.8
30.6 29.2 29.3 32.3 31.0 29.2 34.2 30.7
28.8 28.1
0.9 1.0
1.0
1 .1 1.1 1 .1 1.2
1.0
1.0 1.1
1.0 1 .1 1 .1 1.3
3.89 6.82 4.63 3.95 3.02 4.31 3.49
4.11 5.07 3.03 3.41 2.49
5.06 3.54
INORG PHOS SODIUM
irmol/1
itmol/1
2.16 2.28 2.42
2.32 2.81 2.79 2.64 2.56 2.39
2.21 2.35 2.47 2.36 2.22
141.3 141.8 141.5 142.2 141.9
143.1 141.9
142.3 140.8 142.7 142.8 141.0 141.1
141.0
POTASSIUM umol/1
3.90 3.86 3.90 4.06 4.19 4.49 3.89 4.34 4.14 3.70 3,91 4.53 4.47 3.79
ANIMAL NUMBER
CALCIUM umol/1
CHLORIDE irmol/1
161 2.70 104
162 2.57 101
163 2.62
98
164 2.53 102
165 2.61
99
166 2.54
99
167 2.53 103
168 2.55 101
169 2.55 104
170 2.52 105
171 2.64
98
173 2.52 104
174 2.50 102
175 2.51 103
232
NOTOX Project 242933
CLINICAL BIOCHEMISTRY AFTER. 4 WEEKS FEMALES GROUP 6 (PFOS)
BACK TO MAIN
C H E - I N D - 12 3 0-MAR-9 9
ANIMAL NUMBER
177 178 179 180 181 182 183 184 185 186 187 189 190 191
ALAT(GPT) ASAT(GOT) BILI T.
ukat/1
ukat/1
umol/1
CHOLEST.T. TRIGL.
nrool/1
nmol/1
0.56 0.42 0.72 0.42 0.38 0.63 0.78 0.48 0.51 0.67
0.45 0.57 0.61 1.67
2.04 2.09 2.36 2.48 2.45 1.85 2.32 1.52 2.59 2.95 2.59 2.39 2.77
2.01
2 2
3 4 3 3 4 3 3 3 2 2
2
3
2.56 1.46 2.29 2.14 1.71 2.48 2.19 1.97 1.96 2.00
1.46 . 2.47
2.06
1.83
0.60 0.30 0.38 0.52 0.58 0.82 0.63 0.63 0.58 0.38 0.33 0.65 0.77
0.39
CREATININE GLUCOSE
umol/1
irmol/1
UREA nrool/1
41
5.31
11.3
35
5.90
8.6
38
4.83
7.9
47
5.02 .
12.6
36
5.85
9.1
41
6.81
1 1 .1
43
4.80
9.8
49
5.38
11.6
44
5.12
9.3
39
5.93
9.8
41
6.39
8.2
42
7.96
10.3
37
7.71
8.2
45
6.64
14.4
ANIMAL NUMBER
177 178 179 180 181 182 183 184 185 186 187 189 190 191
PROTEIN T. ALBUMIN g/i g/i
GLOBULIN A/G RATIO ALP 3/1 ukat/1
65.0 66.6
71.5 65.3 67.5 67.5 70.6 68.8 68.8
63.9 63.1 61.2 64.5 70.3
35.1 35.7 33.3 32.6 35.9 34.7 37.6 35.8 35.3 34.0 33.8 32.2 35.0 38.1
29.9 30.9 38.2 32.7 31.6 32.8 33.0 33.0 33.5
29.9 29.3 29.0 29.5 32.2
1.2
1.2 0.9 1.0
1.1
1 .1 1 .1
1.1 1 .1
1.1 1.2
1 .1 1.2
1.2
2.84 3.47
3.85 3.07 3.12
4.80 5.14 4.60 4.12 3.71 7.14 4.32 5.50 4.50
INORG PHDS SODIUM
imol/1
nmol/1
2.55 2.15 2.47
2.69 2.92 2.62 2.64
3.01 2.77 2.74 2.37 2.57 2.36 2.37
143.7 143.4 143.3 142.2 142.9 142.2 142.0 143.4 141.8 144.3 141.9
142.5 141.7
140.0
POTASSIUM nmol/1
4.03 3.80 3.77 4.14 4.09 4.03 3.82 3.77 4.87 4.05 3.84 4.41 4.27 3.95
ANIMAL NUMBER
CALCIUM nmol/1
CHLORIDE imol/1
177 2.58 178 2.54 179 2.59 180 2.56 181 2.59 182 2.73 183 2.72 184 2.62 185 . 2.63 186 2.49 187 2.57 189 2.59 190 2.53 191 2.63
101 102
99 99 102 103 99 104
99 103 104 102 102
99
233
NOTOX Project 242933
CLINICAL BIOCHEMISTRY AFTER 2 WEEKS RECOVERY MALES GROUP 1 (VEHICLE CONTROL)
BACK TO MAIN
C M E - X N D - 13 3 0 - M A R - 99
ANIMAL NUMBER
9 10 11
ALAT(GPT) ASAT(GOT) BILI T.
ukat/1
ukat/1
umol/1
CHOLEST.T. TRIGL.
nnvol/l
xnonol/1
0.82 1.07 0.70
2.50 2.46 3.43
3 3 3
2.35 2 .11 1.39
0.49 0.46 0.63
CREATININE GLUCOSE
umol/1
mmol/1
UREA itmol/ 1
43
5.79
7.2
35
5.21
7.9
28
5.95
5.3
ANIMAL NUMBER
9 10 11
PROTEIN T. ALBUMIN g/i g/i
57.6 54.9 55.1
28.4 28.4 30.1
GLOBULIN A/G RATIO ALP g / i ukat/1
29.2 26.5 25.0
1.0
1.1 1.2
4.77 8.85 6.16
INORG FHOS SODIUM
mmol/1
mnol/1
POTASSIUM innol/1
2.87 2.76 2.80
144.9 144.9 143.0
4.53 4.01 4.85
ANIMAL NUMBER
9 10 11
CALCIUM nxnol/1
CHLORIDE innol/1
2.52 2.44 2.48
103 102 103
234
NOTOX Project 242933
CLINICAL BIOCHEMISTRY AFTER 2 WEEKS RECOVERY MALES GROUP 2 (TELOMER ALCOHOL)
BACK TO MAIN
CHE-IND - 14 3 0 - M AR-99
ANIMAL NUMBER
25 26 27
ALAT(GPT) ASAT(GOT) BILI T.
ukat/1 .ukat/1
urnol/1
0.71 0.58 0.56
2.42 3.16 2.52
3 3 2
CHOLEST.T. TRIGL.
ranol/1
rtmol/1
2.42 1.07 1.53
0.67 0.39 0.77
CREATININE GLUCOSE
umol/1
imol/1
UREA irmol/1
36
7.39
6.8
33
4.33
7.4
32
6.96
7.7
ANIMAL NUMBER
25 26 27
PROTEIN T. ALBUMIN g/i g/i
GLOBULIN A/G RATIO ALP g/i ukat/1
52.0 50.3 52.2
28.6 27.7 28.0
23.4 22.6
24.2
1.2 1.2
1.2
7.04 7.89 5.86
INORG PHDS SODIUM
ninol/1
irmol/1
2.94 2.76 2.63
142.1 145.9 142.3
POTASSIUM ltmol/1
4.59 4.26 4.69
ANIMAL NUMBER
25 26 27
CALCIUM m nol/1
CHLORIDE um ol/1
2.50 2.39 2.44
100 105 102
235
NOTOX Project 242933
-....
CLINICAL BIOCHEMISTRY AFTER 2 WEEKS RECOVERY MALES GROUP 3 (TELOMER ACRYLATE)
BACK TO MAIN
C H E - I N D - 15 3O-MAR-9 9
*
ANIMAL NUMBER
41 42 43
ALAT(GPT) ASAT(GOT) B IL I T .
ukat/1
ukat/1
umol/1
0.61 0.55 0.78
2.89 2.95 2.89
3
3 2
CHOLEST.T. TRIGL.
mnol/1
mnol/1
1.61 1.57 1.54
1.23 0.89 0.54
CREATININE GLUCOSE
umol/1
mmol/1
UREA
mnol/1
31
7.79
6.2
34
6.81
7.5
33
5.94
7.1
ANIMAL
NUMBER
PROTEAN T. ALBUMIN g/i g/ 1
GLOBULIN A/G RATIO ALP g/i ukat/1
41 57.5 42 56.8 43 . 58.6
29.7 29.2 29.6
27.8 27.6 29.0
1.1 1.1 1.0
7.13 4.72 5.60
INORO PHOS SODIUM
mnol/1
mmol/1
POTASSIUM mnol/1
2.98 2.73 2.52
142.6 142.2 141.1
4.97 5.07 4.82
ANIMAL NUMBER
CALCIUM mnol/1
CHLORIDE mmol/1
41 2.51 42 2.46 43 . 2.47
99 102
99
236
NOTOX Project 242933
CLINICAL BIOCHEMISTRY AFTER 2 WEEKS RECOVERY MALES GROUP 4 ( )
BACK TO MAIN
CHE-IND - 1 6 3 0 -MAR-99
ANIMAL NUMBER
57 58 59
ALAT(GPT) ASAT(GOT) BILI T.
ukat/1
ukat/1
umol/1
CHOLEST.T. TRIGL.
mnol/1
mnol/1
0.87 0.66
1.0 1
2.49 3.54 ' 2.56
3 3 2
2.30 2.03 1.45
0.82 0.62 0.67
CREATURINE GLUCOSE
umol/1
tnnol/1
UREA mnol/1
40
8.32
6.7
37
8.45
7.4
40
8.23
6.4
ANIMAI* NUMBER
57 58 59
PROTEIN T. ALBUKEN g/i g/i
52.3 57.3 51.3
27.9 29.1 26.5
GLGBULIN A/G RATIO ALP g /i ukat/1
24.4 28.2 24.8
1 .1
1.0 . 1 .1
6.34 6.70 4.78
INORG FHOS SODIUM
mnol/1
umol/1
2.73 2.68 2.52
140.3 141.2 141.7
POTASSIUM mnol/1 4.35 4.64 4.25
ANIMAL NUMBER
57 58 59
CALCIUM umol/1
CHLORIDE mnol/1
2.57 2.56 2.37
99 101 101
237
NOTOX Project 242933
CLINICAL BIOCHEMISTRY AFTETR 2 WEEKS RECOVERY MALES GROUP 5 (PFHS)
BACK TO MAIN
CHE-IND - 17 3 0 -MAR-99
ANIMAL NUMBER
73 74 75
ALAT(GPT) ASAT(GOT) BILI T.
ukat/1
ukat/1
umol/1
CHDLEST.T. TRIGL.
nmol/l
nraol/1
0.71 0.96 0.75
2.82 2.49 1.99
2
2 2
1.29 1.63 1 .2 1
0.58 0.62 0.75
CREATININE GLUCOSE
umol/l
nmol/l
UREA. nmol/l
39
7.25
6.6
42
6.96
7.6
35
7.21
6.0
ANIMAL NUMBER
73 74 75
PROTEIN T. ALBUMIN g/1 g/1
GLOBULIN A/G RATIO ALP g/i ukat/1
53.9 55.1 55.1
28.4 28.2 30.0
25.5 26.9 25.1
1.1 1.0 1.2
5.85 6.89 7.43
INORG PHOS SODIUM
nmol/l
nmol/l
2.67 2.43 2.42
142.1 143.4 141.7
POTASSIUM nmol/l 4.71 4.57 4.41
ANIMAL NUMBER
73 74 75
CALCIUM n m ol/l
CHLORIDE n m ol/l
2.44 2.49 2.42
101
104 100
238
NOTOX Project 242933
CLINICAL BIOCHEMISTRY AFTER 2 WEEKS RECOVERY MALES GROUP 6 (PFOS)
BACK TO MAIN
C H E - I N D - 18 30-MAR-9 9
ANIMAL NUMBER
ALAT(GPT) ASAT(GOT) BILI T.
ukat/1
ukat/1
umol/1
CHOLEST.T. TRIGL.
mtiol/1
nmol/1
89 0.83 90 0.61 91 ' 0.49
2.48 1.63 1.66
2
3 3
1.22
1.48 1.12
0.51 0.40 0.60
CREATININE GLUCOSE
umol/1
nmol/1
UREA nmol/1
34
7.21
7.3
34
6.29
6.7
49
5.17
8.1
ANIMAL tJMBER
89 90 91
PROTEIN T. ALBUMIN g/i g/l
GLOBULIN A/G RATIO ALP 3/1 ukat/1
56.3 55.9 57.3
30.0 29.0 30.1
26.3 26.9 27.2
1 .1 1 .1 1 .1
6.36 4.22 6.26
INORG PHOS SODIUM
nmol/1
nmol/1
POTASSIUM nmol/1
2.52 2.67 2.75
142.8 142.7 142.8 .
4.52 4.82 4.35
ANIMAL NUMBER
89 90 91
CALCIUM nmol/1
CHLORIDE mnol/1
2.53 2.65 2.53
101 102
3-05
239
NOTOX Project 242933
____
CLINICAL BIOCHEMISTRY AFTER 2 WEEKS RECOVERY
FEMALES GROUP 1 (VEHICLE CONTROL}
BACK TO MAIN
CHE-IND - 19 3 0 - M A R - 99
ANIMAL NUMBER
105 106 107
ALAT(GPT) ASAT(GOT) BILI T.
ukat/1
ukat/1
umol/1
CHOLEST.T. TRIGL.
mtnol/1
mnol/1
0.52 0.43 0.46
2.50 1.96 3.12
3'. 3 2
1.95 2.59 1.93
0.56 0.97 0.55
CREATININE GLUCOSE
umol/1
mnol/1
UREA mnol/1
37
6.72
6.7
38
9.25
6.2
41
6.06
7.6
ANIMAL NUMBER
105 106 107
PROTEIN T. ALBUMIN g/i g/i
65.2 65.3 60.4
32.4 34.7 29.4
GLOBULIN A/G RATIO ALP g/i ukat/1
32.8 30.6 31.0
1.0
1.1 0.9
3.43 3.21 3.26
INORG PHDS SODIUM
mnol/1
mnol/1
POTASSIUM mnol/1
2.45 2.39 2.56
143.3 142.5 141.4 .
3.98 4.14 4.12
ANIMAL NUMBER
105 106 107
CALCIUM mnol/1
CHLORIDE rnicl/1
2.60 2.79 2.51
98 99 99
240
NOTOX Project 242933
CLINICAL BIOCHEMISTRY . AFTER 2 WEEKS RECOVERY FEMALES GROUP 2 (TELOMER ALCOHOL)
BACK TO MAIN
CHE-IND - 20 3 0 - M A R - 99
ANIMAL NUMBER
ALAT(GPT) ASAT(GOT) BILI T.
ukat/1
ukat/1
umol/1
121 0.52 122 1.02 123 . 0.36
2.44 3.36 2.02
2 3 2
CHOLEST.T. TRIGL.
mnol/1
mnol/1
2.14 2.20 1.42
0.43 0.40 0.46
CREATININE GLUCOSE
umol/1
nrool/1
UREA mmol/1
40
7.26
6.3
41
6.31
8.9
38
5.98
6.7
ANIMAL NUMBER
121 122 123
PROTEIN T. ALBUMIN g/i g/i
GLOBULIN A/G RATIO ALP g/i ukat/1
58.1 61.3 65.6
29.6 34.9 33.6
28.5 26.4 32.0
1.0
1.3 1.1
4.38 4.07 2.34
INORG PHDS SODIUM
mnol/1
mnol/1
2.64 2.89 2.18
142.4' 144.2 141.7
POTASSIUM mnol/1 3.95 4.16 3.77
ANIMAL NUMBER
121 122 123
CALCIUM mnol/1
CHLORIDE ranol/1
2.54 2.65 2.59
98 100
99
241
NOTOX Project 242933
CLINICAL BIOCHEMISTRY . AFTER 2 WEEKS RECOVERY FEMALES GROUP 3 (TELOMER ACRYLATE)
BACK TO MAIN
C H E - X N D - 21 3 0 - M A R - 99
ANIMAL
NUMBER
137 138 139
ALAT(GPT) ASAT(GOT) BILI T.
ukat/1
ukat/1
umol/1
0.58 0.51 0.37
2.27 2.35 1.69
2 2 2
CHDLEST.T. TRIGL.
nrool/1
innol/1
1.27 1.97 1.97
0.39 0.87 0.57
CREATININE GLUCOSE
umol/1
mmol/l
UREA nmol/1
37
6.71
7.1
40
7.44
9.3
47
7.77
8 .1
ANIMAL
NUMBER
137 138 139
PROTEIN T. ALBUMIN ff/1 g / i
60.7 62.9 59.7
29.5 32.5 32.9
GLOBULIN A/G RATIO ALP g / l ukat/1
31.2 30.4 26.8
0.9 1.1
1.2
5.56 4.02 3.49
INORG PROS SODIUM
rnnol/1
sin o l/1
2.27 2.38 2.30
143.1 141.4 141.5
POTASSIUM mmol/1
3.76 4.54 3.86
ANIMAL
NUMBER
137 138 139
CALCIUM imcl/1
CHLORIDE umol/1
2.46 2.61 2.63
98 103
96
242
NOTOX Project 242933
CLINICAL BIOCHEMISTRY AFTER 2 WEEKS RECOVERY FEMALES GROUP 4 ( )
_ '
3
BACK TO MAIN
C H E - I N D - 22 0-MAR-99
ANIMAL NUMBER
153 154 155
ALAT(GPT) ASAT(GOT) BILI T. ' CHQLEST.T. TRIGL.
ukat/1
ukat/1
umol/1
irmol/1
nmol/1
0.48 0.63 0.60
2.35 2.32 2.93
3 2 3
2.17 2.72
2.34
1.03 0.53
0.63
CREATININE GLUCOSE
umol/1
nmol/1
UREA. nmol/1
43
7.47
9.5
39
8.41
8.9
53
6.34
11.0
ANIMAL NUMBER
153 154 155
PROTEIN T. ALBUMIN g/i 9/1
GLOBULIN A/G RATIO ALP g/i ukat/1
67.8 61.8 66.1
35.7 32.4 33.4
32.1 29.4 32.7
1 .1 1 .1 1.0
3.09 4.81 3.65
INORG PHDS SODIUM
nmol/1
nmol/1
POTASSIUM nmol/1
2.70 2.23 2.71
142.7 139.7 141.4
3.90 4.61 4.50
ANIMAL NUMBER
153 154 155
CALCIUM nmol/1
CHLORIDE
nmol/1
.
2.69 2.64 2.70
100
98 97
.'
243
NOTOX Project 242933
CLINICAL BIOCHEMISTRY AFTER 2 WEEKS RECOVERY FEMALES GROUP 5 (PFHS)
_ "
BACK TO MAIN
C H E - I N D - 23 30-MAR-99
*
ANIMAL NUMBER
169 170 171
ALAT(GPT) ABAT (GOT) BILI T.
ukat/1
ukat/1
umol/1
CHDLEST.T. TRIGL.
nmol/l
irmol/1
0.48 0.43 0.65
1.83 1.56 1.58
3 3 .3
1.66
2.42 2.24
0.50 0.35 0.34
CREATININE GLUCOSE
uxnol/l
nmol/l
UREA irmol/1
46
7.25
7.7
39
6.01
7.2
47
9.38
7.9
ANIMAL NUMBER
169 170 171
PROTEIN T. ALBUMIN 3/1 g / i
GLOBULIN A/G RATIO ALP g / i ukat/1
65.5 62.8 71.0
30.7 30.9 32.5
34.8 31.9 38.5
0.9 1.0 0.8
4.62 2.30 2.90
INORO PROS SODIUM
nmol/l
nmol/l
2.37 2.19 2.70
141.1 141.1 142.5
POTASSIUM nmol/l 3.98 3.65 4.08
ANIMAL NUMBER
169 170 171
CALCIUM nmol/l
CHLORIDE nmol/l
2.58 2.62 2.84
100 99 95
244
NOTOX Project 242933
CLINICAL BIOCHEMISTRY AFTER 2 WEEKS RECOVERY
6FEMALES
GROUP (PFOS)
BACK TO MAIN
CHE-IND - 24 3 0-MAR-99
ANIMAI, NUMBER
185 186 187
ALAT(GPT) ASAT(GOT) BILI T.
ukat/1
ukat/1
umol/1
0.37
0.51 0.48
2.22 2.15 1.89
2 3 2
CHQLEST.T. TRIGL.
nmol/1
mmol/1
2.04 2.37 1.64
0.40 0.25 0.29
CREATININE GLUCOSE
umol/1
nmol/l
UREA umol/1
-43
8.03
8.2
41
7.67
9.0
42
7.37
6.7
ANIMAL
NUMBER
185 186 187
PROTEIN T. ALBUMIN ff/1 g / i
66.6
57.4 57.9
33.9 31.6 32.0
GLOBULIN A/G RATIO ALP g n ukat/1
32.7 25.8 25.9
1 .0
1.2 1.2
3.66 3.25 6.04
INORG PHOS SODIUM
nmol/1
nmol/1
2.40 2.21
2.27
141.9 143.4 142.2
POTASSIUM nmol/1
3.97 4.27 4.08
ANIMAL
NUMBER
185 186 187
CALCIUM nmol/1
CHLORIDE nmol/1
2.65 2.56 2.69
99 103
99
245
NOTOX Project 242933
_
CLINICAL BIOCHEMISTRY AFTER 4 WEEKS RECOVERY
MALES GROUP 1 (VEHICLE CONTROL)
BACK TO MAIN
CHE-IND - 25 30-MAR-99
*
ANIMAL NUMBER
13 14 15
ALAT(GET) ASAT(GOT) BILI T.
ukat/1
ukat/1
umol/1
0.78 0.54 0.44
2.88
2.39 2.69
4 3 3
CHOLEST.T. TRIGL.
nrool/1
ltmol/1
2.07 2.01
1.73
1.17
0.39 0.71
CREATININE GLUCOSE
umol/1
nmol/1
UREA itmol/ 1
37
8.42
6 .1
35
7.63
6 .1
37
7.97
6.7
ANIMAL NUMBER
13 14 15
PROTEIN T. ALBUMIN g/i g/i
62.1
62.2 62.2
29.3
28.2 28.4
GLOBULIN A/G RATIO ALP g / i ukat/1
32.8 34.0
33.8
0.9 0.8 0.8
4.61 4.79
3.58
INORG PHOS SODIUM
nmol/1
nmol/1
2.91 3.17
2.99
140.8 142.5 141.6
POTASSIUM nmol/1
5.15 5.56 5.28
ANIMAL NUMBER
13 14 15
CALCIUM nmol/1
CHLORIDE nmol/1
2.56 2.60 . 2.58
104 101 102
246
NOTOX Project 242933
CLINICAL BIOCHEMISTRY . AFTER 4 WEEKS RECOVERY MALES GROUP 2 (TELOMER ALCOHOL)
--"
BACK TO MAIN
CHE-IND - 26 30-MAR-99
.
ANIMAL NUMBER
ALAT(GPT) ASAT(GOT) BILI T.
ukat/1
ukat/1
umol/1
CHQLEST.T. TRIGL.
ranol/1
mnol/1
29 0.70 2.66 2 30 0.61 2.49 2 31 0.74 2.17 3
1.39 1.22
1.14
0.57 0.55
0.61
CREATININE GLUCOSE
umol/1
mnol/1
UREA mnol/1
29
5.87
6.5
33
8.32
6.3
36
8.16
6.2
ANIMAL NUMBER
29 30 31
PROTEIN T. ALBUMIN g/1 g/1
GLOBULIN A/G RATIO ALP g/1 ukat/1
INORG PHOS SODIUM
mnol/1
nmol/1
57.8 54.2 55.6
26.7 27.5 27.7
31.1 26.7 27.9
0.9 1.0 1.0
6.15 8.13 4.31
2.44 2.51 2.96
143.4 141.4 143.9
POTASSIUM nnvol/1
4.53 4.50 4.75
ANIMAL NUMBER
29 30 31
CALCIUM mnol/1
CHLORIDE mmol/1
2.44 2.46 2.55
106
103 101
247
NOTOX Project 242933
CLINICAL BIOCHEMISTRY AFTER 4 WEEKS RECOVERY MALES GROUP 3 (TELOMER ACRYLATE)
BACK TO MAIN
CHE-IND - 27 3 0 - M A R - 99
ANIMAL NUMBER
45 46 47
ALAT(GPT) ASAT(GOT) BILI T.
ukat/1
ukat/1
umol/1
0.62 0.57 0.60
2.00
2.33 1.86
3 2 3
CHOLEST.T. TRIGL.
ittnol/1
mmol/l
1.93 1.16 1.51
0.42 0.53 0.64
CREATININE GLUCOSE umol/1 . mmol/l
UREA mmol/l
32
7.91
7.4
39
7.38
6.7
31
5.88
5.6
ANIMAL
NUMBER
45 46 47
PROTEIN T. ALBUMIN g/l g/i
58.4 51.6 56.2
27.7 25.4 27.9
GLOBULIN A/G RATIO ALP ' g/l ukat/1
30.7 26.2 28.3
0.9 1.0 1.0
3.83 6.75 5.27
INORG PHDS SODIUM
mmol/l
mmol/l
2.67 2.86
2.38
141.4 143.8 142.3
POTASSIUM rnnol/ 1
4.86 4.81 4.26
ANIMAL NUMBER
CALCIUM mmol/l
CHLORIDE ncnol/l
45 2.53 102 46 2.47 105 47 2.44 104
24 8
NOTOX Project 242933
CLINICAL BIOCHEMISTRY . AFTER 4 WEEKS RECOVERY MALES GROUP 4 ( )
BACK TO MAIN
C H E - I N D - 28 3 0 - M A R - 99
ANIMAL
NUMBER
61 62 63
ALAT(GET) ASAT(GOT) BILI T.
ukat/1
ukat/1
umol/1
0.67 0.38 0.69
2.96 2.16
2.05
3 2
3
CHQLEST.T. TRIGL.
nmol/1
nmol/1
1.37
2.05 1.56
0.55 0.59 0.55
CREATININE GLUCOSE
umol/1
nmol/1
UREA nmol/1
39
5.58
6.9
37
6.68
8.2
39
6 .11
7.3
ANIMAL NUMBER
61 62 ' 63
PROTEIN T. ALBUMIN g/i g/i
GLOBULIN A/G RATIO ALP g/1 ukat/1
59.5 54.9 56.6
29.0 27.7 26.5
30.5 27.2 30.1
1.0 1.0
0.9
4.68 4.66 6.94
INORG PHDS SODIUM
POTASSIUM
umol/1
umol/1 . nmol/1
2.46 2.64 2.28
. 141.5 141.8 142.7
4.61 4.62 4.41
ANIMAL
NUMBER _>
61 62 63
CALCIUM nmol/1
CHLORIDE nmol/1
2.49 2.45 2.44
103 . 104
105
249
NOTOX Project 242933
CLINICAL BIOCHEMISTRY . AFTER 4 WEEKS RECOVERY MALES GROUP 5 (PFHS)
BACK TO MAIN
CKE-IND - 29 30-MAR-99
ANIMAL NUMBER
77 78 79
ALAT(GPT) ASAT(GOT) BILI T.
ukat/1
ukat/1
umol/1
CHOLEST.T. TRIGL.
nmol/1
irtnol/1
0.60 0.94 0.71
2.61 2.21 2.38
3 2
2
1.22 1.34 1.16
0.57 0.26 0.24
CREATININE GLUCOSE
umol/1
nxnol/ 1
UREA nmol/1
47
5.95
8.1
47
4.77
10.4
36
6.00
6.5
ANIMAL NUMBER
77 78 79
PROTEIN T. ALBUMIN g/i g/i
GLOBULIN A/G RATIO ALP g / i ukat/1
57.9 58.1 56.0
27.0 28.0 27.0
30.9 30.1 29.0
0.9 0.9 0.9
5.79 4.17 8.04
INORO PHOS SODIUM
nmol/1
nmol/1
2.55 2.55 2.86
143.5 142.9 144.9
POTASSIUM nmol/1
4.40 4.26 5.04
ANIMAL NUMBER
77 78 79
CALCIUM nmol/1
CHLORIDE mnol/1
2.48 2.49 2.51
104 103 103
250
NOTOX Project 242933
CLINICAL BIOCHEMISTRY AFTER 4 WEEKS RECOVERY HALES GROUP 6 (PFOS)
BACK TO MAIN
C H E - I N D - 30 3 0 - M A R - 99
ANIMAL NUMBER
93 94 95
ALAT(GPT) ASAT(GOT) BILI T.
ufcat/1
ukat/l
urool/1
CHDLEST.T. TRIGL.
mmol/1
umol/1
0.56 0.77 0.58
1.99 1.73 1.89
2 2 2
0.92 1.47 1.62
0.35 0.58 0.43
CREATININE GLUCOSE
umol/1
nrool/1
UREA mmol/1
29
6.01
5.8
31
7.23
5.6
36
6.85
6.5
ANIMAL NUMBER
93 94 95
PROTEIN T. ALBUMIN 3/1 g / i
51.7 55.0 56.7
27.0 27.3 28.3
GLOBULIN A/G RATIO ALP g /1 ukat/l
24.7 27.7 28.4
1.1 1.0 1.0
4.89 4.78 4.52
INORG PHDS SODIUM
mnol/1
mmol/1
2.58 2.40 2.79
142.8 142.7 ' 142.5
POTASSIUM mcnol/ 1
4.60 4.78 4.58
ANIMAL NUMBER
CALCIUM ninol/1
CHLORIDE mmol/1 .
93 - 2.43 94 2.52 95 2.49
105 107 109
251
NOTOX Project 242933
CLINICAL BIOCHEMISTRY AFTER 4 WEEKS RECOVERY FEMALES GROUP 1 (VEHICLE CONTROL)
_
BACK TO MAIN
CHE-IND - 31 3 0 - M A R - 99
ANIMAL NUMBER
109 110 111
ALAT(GPT) ABAT(GOT) BILI T.
ukat/1
ukat/1
umol/1
0.41
0.45 0.28
2.28 2.54
1.92
3 2
2
CHOLEST.T. TRIGL.
mmol/1
mmol/1
2.25
2.31 2.94
0.60
0.78 0.52
CREATININE GLUCOSE umol/1 . mmol/1
UREA mmol/1
45
6.19
7.7
40
8.58
5.9
39
6.70
9.0
ANIMAL NUMBER
109 110 111
PROTEIN T. ALBUMIN g/i 0/1
61.4 69.4 63.8
30.1 33.2 30.5
GLOBULIN A/G RATIO ALP 0/1 ukat/1
31.3 36.2 33.3
1.0 0.9 0.9
3.41 2.49 2.96
INORG PHDS SODIUM mmol/1 . mmol/1
2.46 2.14 2.39
144.0 142.5 140.5
POTASSIUM mmol/1
3.84 4.01 4.50
ANIMAL
CALCIUM
NUMBER itmol/1
CHLORIDE mmol/1
109 2.47 103
110 2.55 101
1 1 1 2.55
96
.
252
NOTOX Project 242933
CLINICAL BIOCHEMISTRY . AFTER 4 WEEKS RECOVERY FEMALES GROUP 2 (TELOMER ALCOHOL)
--
BACK TO MAIN
CHE-IND - 32 30-MAR-99
.
ANIMAL NUMBER
125 126 127
AIiAT(GFT) ASAT(GOT) BILI T.
ukat/1
ukat/1
umol/1
CHOLEST.T. TRIGL.
mnol/1
mnol/1
0.39 0.34
--
2.66
2.26 2.01
2 2
3
2.68 2.01
2.55
1.00
0.47 0.76
CREATININE GLUCOSE
umol/1
mmol/1
UREA mnol/1
39
6.83
6.7
40
6.54
6.8
48
5.91
9.0
ANIMAL NUMBER
125 126 127
PROTEIN T. ALBUMIN g/i g/i
67.0 62.2 63.6
32.2 30.3 32.4
GLOBULIN A/G RATIO ALP g / i ukat/1
34.8 31.9 31.2
' 0.9 0.9 1.0
1.73 3.18 4.19
INORO PHDS SODIUM
mnol/1
mnol/1
2.26 2.35
2 .11
141.4
142.2 141.3
POTASSIUM mnol/1
4.25 4.36 3.82
ANIMAL NUMBER
125 126
CALCIUM mnol/1
CHLORIDE mnol/1
2.64 2.49
104 98
NOTOX Project 242933
CLINICAL BIOCHEMISTRY . AFTER 4 WEEKS RECOVERY FEMALES GROUP 3 (TELOMER ACRYLATE)
BACK TO MAIN
C H E - I N D - 33 3 0 - M A X - 99
ANIMAL NUMBER
141 142 143
ALAT(GPT) ASAT(GOT) BILI T.
ukat/1
ukat/1
umol/1
CHDLEST.T. TRIGL.
nmol/1
nmol/1
0.43 0.36 0.59
1.74 1.54 2.40
2 3 3
2.18 2.39 2.32
0.43 0.50 0.49
CREATININE GLUCOSE
umol/1
itmol/1
UREA nmol/1
40
6.64
8.3
41
6.73
6.9
45
7.32
8.8
ANIMAL NUMBER
141 142 143
PROTEIN T. ALBUMIN g /1 g/i
65.5 67.8 69.9
29.5 34.7 35.9
GLOBULIN A/G RATIO ALP g/i ukat/1
36.0 33.1 34.0
0.8 1.0 1 .1
3.86 2.08 3.40
INORG PHDS SODIUM
nmol/1
zrmol/1
2.19 1.95 1.79
138.7 141.6 141.7
POTASSIUM nmol/1 5.43 3.66 4.34
ANIMAL NUMBER
141 142 143
CALCIUM imol/l
CHLORIDE mnol/1
2.47 2.60
2.59
96 103 102
254
NOTOX Project 242933
CLINICAL BIOCHEMISTRY . AFTER 4 WEEKS RECOVERY FEMALES GROUP 4 ( )
~
BACK TO MAIN
CHE-IND - 34 30-MAR-99
.
ANIMAL NUMBER
157 158 159
ALAT(GPT) ASAT(GOT) BILI T.
ukat/1
ukat/1
umol/1
CHOLEST.T. TRIGL.
itmol/1
itmol/1
0.43 0.98 0.47
2.75 2.30 2.31
2
2 2
2.99 2.52 2.02
0.41 0.40 0.72
CREATININE GLUCOSE
umol/1
irmol/1
UREA mmol/1
43
5.89
8.2
40
8.08
6.9
48
5.05
9.9
ANIMAL NUMBER
157 158 159
PROTEIN T. ALBUMIN g/1 g/i
GLOBULIN A/G RATIO ALP g/i ukat/1
64.8 68.9 62.2
30.3
34.5
32.0
36.9
29.4 ' 32.8
0.9 0.9 0.9
2.48 3.91 2.40
INORO PHDS SODIUM
ranol/1
umol/1
2.20 1.93 2.52
141.0 142.1 140.9
POTASSIUM mmol/1 4.51 3.67 4.59
ANIMAL NUMBER
157 158 159
CALCIUM mmol/1
CHLORIDE mmol/1
2.60 2.57 2.53
100 96
101
2 55
NOTOX Project 242933
CLINICAL BIOCHEMISTRY . AFTER 4 WEEKS RECOVERY FEMALES GROUP 5 (PFHS)
BACK TO MAIN
CHE-IND - 35 3 0 - M A R - 99
ANIMAL NUMBER
173 174 175
ALAT(GPT) ASAT(GOT) BILI T.
ukat/1
ukat/1
umol/1
CHOLEST.T. raiGL.
nmol/l
nmol/l
0.33 0.64 0.45
2.27
3.09 2.21
2 2 2
1.68 2.12 2.37
0.40
0.34 0.46
CREATININE GLUCOSE
umol/1
nmol/l
UREA nmol/l
38
6.77
6.8
39
6.50
6.9
39
7.31
7.3
ANIMAL NUMBER
173 174 175
PROTEIN T. ALBUMIN g/1 g/1
68.5 69.4 70.2
34.9 33.5 34.2
GLOBULIN A/G RATIO ALP g/i ukat/1
33.6 35.9 36.0
1.0 0.9 1.0
1.78 3.46 2.85
INORG PHDS SODIUM
nmol/l
nmol/l
2.15 2.08 2.24
139.9 140.5 142.6
POTASSIUM irmol/1
4.17 4.25 3.65
ANIMAL NUMBER
173 174 175
CALCIUM nmol/l
CHLORIDE nmol/l
2.59 2.59 2.61
104 99
102
256
NOTOX Project 242933
CLINICAL BIOCHEMISTRY AFTER 4 WEEKS RECOVERY FEMALES GROUP 6 (PFOS)
BACK TO MAIN
CHE-IND - 3S 3 0-M A R -9 9
ANIMAL NUMBER
189 190 191
ALAT(GPT) ASAT(GOT) BILI T.
ukat/1
ukat/1
umol/1
CHOLEST.T. TRIGL.
mmol/1
nrool/1
0.52 0.49 0.50
1.96 1.94 1.86
2 2
3
2.38 1.99 2.01
0.39 0.74 0.37
CREATININE GLUCOSE
unol/l
nmol/1
UREA nmol/1
39
6.77
9.4
38
8.87
7.9
39
7.31
14.9
ANIMAL NUMBER
189 190 191
PROTEIN T. ALBUMIN 3/1 3/1
59.5 66.2 70.1
30.6 33.2 34.6
GLOBULIN A/G RATIO ALP 3/1 ukat/1
28.9 33.0 35.5
1 .1 1.0 1.0
3.56 3.19 3.40
INORG PHDS SODIUM
nmol/1
nmol/1
2.56 2.18 2.07
142.7 141.0 139.5
POTASSIUM nmol/1
4.13 4.36 4.00
ANIMAL NUMBER
189 190 191
CALCIUM nmol/1
CHLORIDE nmol/1
2.63 2.61 2.67
100 108 100
2 57
NOTOX Project 242933
TELOMER ALCOHOL/TELOMER ACRYLATE/
-/PFHS/PFOS
'
BACK TO MAIN
MAC-IND - 1 25-FEB-99
MACROSCOPIC FINDINGS MALES GROUP 1 (VEHICLE CONTROL)
ANIMAL 1
NO FINDINGS NOTED
ANIMAL 2
NO FINDINGS NOTED
ANIMAL 3
NO FINDINGS NOTED
ANIMAL 4
NO FINDINGS NOTED
ANIMAL 5
NO FINDINGS NOTED
ANIMAL 6
NO FINDINGS NOTED
ANIMAL 7
NO FINDINGS NOTED
ANIMAL 8
NO FINDINGS NOTED
ANIMAL 9
SKIN...............
CHEST REGION: ALOPECIA.
ANIMAL 10
NO FINDINGS NOTED
ANIMAL 11
NO FINDINGS NOTED
* (SCHEDULED NECROPSY, 03-N0V-98) (SCHEDULED NECROPSY, 03-N0V-98) (SCHEDULED NECROPSY, 03-NOV-98) (SCHEDULED NECROPSY, 03-NOV-98) (SCHEDULED NECROPSY, 04-NOV-98) (SCHEDULED NECROPSY, 04-N0V-93) (SCHEDULED NECROPSY, 04-NOV-98) (SCHEDULED NECROPSY. 04-NOV-98) (SCHEDULED NECROPSY, 18-N0V-98) (SCHEDULED NECROPSY. 18-N0V-98) (SCHEDULED NECROPSY, 1B-N0V-98)
25 8
NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/
'/PFHS/PFOS
"
'
BACK TO MAIN
MAC-IND - 2. 25-FEB-99
MACROSCOPIC FINDINGS MALES GROUP 1 (VEHICLE CONTROL) ANIMAL 13
NO FINDINGS NOTED
ANIMAL 14
NO FINOINGS NOTED
ANIMAL 15
NO FINDINGS NOTED
.
. (SCHEDULED NECROPSY, 02-DEC-98) (SCHEDULED NECROPSY, 02-DEC-98)
(SCHEDULED NECROPSY, 02-DEC-98)
259
NOTOX Project 242933
'-
TELOMER ALCOHOL/TELOMER ACRYLATE/ _ ,/PFHS/PFOS
"
BACK TO MAIN
MAC-IND - 3 25-FEB-99
MACROSCOPIC FINDINGS MALES GROUP 2 (TELOMER ALCOHOL)
.
ANIMAL 17
NO FINDINGS NOTED
ANIMAL 18
NO FINDINGS NOTED
ANIMAL 19
`
NO FINDINGS NOTED
ANIMAL 20
NO FINOINGS NOTED
ANIMAL 21
NO FINOINGS NOTED
ANIMAL 22
NO FINDINGS NOTED
ANIMAL 23
NO FINDINGS NOTED
ANIMAL 24 NO FINDINGS NOTED
ANIMAL 25
.
NO FINDINGS NOTED
ANIMAL 26
NO FINDINGS NOTED
ANIMAL 27
TESTES.............
REDUCED IN SIZE, FLACCID.
(SCHEDULED NECROPSY, 03-NOV-98) (SCHEDULED NECROPSY, 03-NOV-98) (SCHEDULED NECROPSY, 03-N0V-98) (SCHEDULED NECROPSY, 03-N0V-93) (SCHEDULED NECROPSY, 04-N0V-9B) (SCHEDULED NECROPSY, 04-N0V-98) (SCHEDULED NECROPSY, 04-N0V-98) (SCHEDULED NECROPSY, 04-N0V-98) (SCHEDULED NECROPSY, 18-N0V-98) (SCHEDULED NECROPSY, 18-N0V-98) (SCHEDULED NECROPSY, 18-N0V-98)
260
NOTOX Project 242933
*-
TELOMER ALCOHOL/TELOMER ACRYLATE/ _ /PFHS/PFOS
"
BACK TO MAIN
MAC-IND - 4 25-FEB-99
MACROSCOPIC FINDINGS MALES GROUP 2 (TELOMER ALCOHOL) ANIMAL 29
NO FINDINGS NOTED
ANIMAL 30
NO FINDINGS NOTED
ANIMAL 31
NO FINDINGS NOTED
(SCHEDULED NECROPSY, 02-DEC-98) (SCHEDULED NECROPSY, 02-DEC-98) ' (SCHEDULED NECROPSY, 02-DEC-98)
261
NOTOX P r o je c t 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/
-/PFHS/PFOS
BACK TO MAIN
MAC-IND - 5 25-FEB-99
MACROSCOPIC FINDINGS MALES GROUP 3 (TELOMER ACRYLATE) ANIMAL 33 NO FINDINGS NOTED ANIMAL 34 NO FINDINGS NOTED ANIMAL 35 NO FINDINGS NOTED ANIMAL 36 NO FINDINGS NOTED ANIMAL 37 NO FINDINGS NOTED ANIMAL 38 NO FINDINGS NOTED ANIMAL 39 NO FINDINGS NOTED ANIMAL 40 NO FINDINGS NOTED ANIMAL 41 NO FINDINGS NOTED ANIMAL 42 NO FINDINGS NOTED ANIMAL 43 NO FINDINGS NOTED
.
(SCHEDULED NECROPSY, 03-N0V-98) (SCHEDULED NECROPSY, 03-N0V-98) (SCHEDULED NECROPSY, 03-N0V-98) (SCHEDULED NECROPSY, 03-NOV-98) (SCHEDULED NECROPSY, 04-NOV-98) (SCHEDULED NECROPSY, 04-NOV-98) (SCHEDULED NECROPSY, 04-NOV-98) (SCHEDULED NECROPSY. 04-N0V-98) (SCHEDULED NECROPSY, 18-N0V-98) (SCHEDULED NECROPSY, 18-N0V-98) (SCHEDULED NECROPSY, 18-N0V-98)
262
NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/
-/PFHS/PFOS
`
BACK TO MAIN
MAC-IND - 6 2 5 - F E B -99
MACROSCOPIC FINDINGS MALES GROUP 3 (TELOMER ACRYLATE) ANIMAL 45
NO FINDINGS NOTED
ANIMAL 46
NO FINDINGS NOTED
ANIMAL 47
NO FINDINGS NOTED
{SCHEDULED NECROPSY, 02-DEC-98) (SCHEDULED NECROPSY, 02-DEC-93) (SCHEDULED NECROPSY, 02-0EC-93)
263
NOTOX Project 242933
--
TELOMER ALCOHOL/TELOMER ACRYLATE. _ JPFHS/PFOS
'
BACK TO MAIN
MAC-IND - 7 25-FEB-99
MACROSCOPIC FINDINGS
MALES
GROUP 4 (
)
ANIMAL 49
.
NO FINDINGS NOTED
ANIMAL SO
NO FINDINGS NOTED
ANIMAL 51
NO FINDINGS NOTED
ANIMAL 52
NO FINDINGS NOTED
ANIMAL 53
NO FINDINGS NOTED
ANIMAL 54
NO FINDINGS NOTED
ANIMAL 55
NO FINDINGS NOTED ANIMAL 56
NO FINDINGS NOTED
.
ANIMAL 57
NO FINDINGS NOTED
ANIMAL 58
NO FINDINGS NOTED
ANIMAL 59
,
NO FINOINGS NOTED
(SCHEDULED NECROPSY, 03-N0V-98)
(SCHEDULED NECROPSY, 03-N0V-98)
(SCHEDULED NECROPSY, 03-N0V-98)
(SCHEDULED NECROPSY, 03-N0V-98)
(SCHEDULED NECROPSY, 04-NOV-98)
(SCHEDULED NECROPSY, 04-N0V-98)
(SCHEDULED NECROPSY, 04-NOV-98) (SCHEDULED NECROPSY, 04-N0V-98)
(SCHEDULED NECROPSY, 18-N0V-98)
(SCHEDULED NECROPSY, 18-N0V-98)
(SCHEDULED NECROPSY, 18-N0V-98)
.
264
NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/
-/PFHS/PFOS
"
BACK TO MAIN
MAC-IND - 8 25-FEB-99
MACROSCOPIC FINDINGS
MALES
GROUP4 (
)
ANIMAL 61
EYES........
RIGHT SIDE, LENS: REDUCED IN SIZE.
ANIMAL 62
NO FINDINGS NOTED
ANIMAL 63
NO FINDINGS NOTED
(SCHEDULED NECROPSY, 02-DEC-98) (SCHEDULED NECROPSY, 02-DEC-98) (SCHEDULED NECROPSY, 02-DEC-98)
265
NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/
-/PFHS/PFOS
MACROSCOPIC FINDINGS MALES GROUP 5 (PFHS)
ANIMAL 65
NO FINDINGS NOTED
ANIMAL 66
LUNGS.............. STOMACH............ TESTES............. EPIDIDYMIDES.......
HEMORRHAGE. GLANDULAR MUCOSA: HEMORRHAGE. REDUCEO IN SIZE. REDUCED IN SIZE.
ANIMAL 67
NO FINDINGS NOTED
ANIMAL 68
NO FINDINGS NOTED
ANIMAL 69
NO FINDINGS NOTED
ANIMAL 70
NO FINDINGS NOTED
ANIMAL 71 NO FINDINGS NOTED
ANIMAL 72
LIVER..............
ENLARGED.
ANIMAL 73
NO FINDINGS NOTED
ANIMAL 74
TESTES.............
REDUCED IN SIZE.
BACK TO MAIN
MAC-IND 9 2 5 - F E B -99
(SCHEDULED NECROPSY, 03-N0V-98) (SCHEDULED NECROPSY. 03-N0V-98)
(SCHEDULED NECROPSY, 03-NOV-9B) (SCHEDULED NECROPSY, 03-NOV-98) (SCHEDULED NECROPSY. 04-NOV-98) (SCHEDULED NECROPSY, 04-NOV-98) (SCHEDULED NECROPSY, 04-NOV-98) (SCHEDULED NECROPSY, 04-N0V-98) (SCHEDULED NECROPSY, 18-N0V-98) (SCHEDULED NECROPSY, 18-N0V-98)
266
NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/
-_ /PFHS/PFOS
MACROSCOPIC FINDINGS MALES GROUP 5 (PFHS) ANIMAL 75
NO FINDINGS NOTED
ANIMAL 77
NO FINDINGS NOTED
ANIMAL 78
NO FINDINGS NOTED
ANIMAL 79
NO FINDINGS NOTED
.
BACK TO MAIN
MAC-IND -10 25-FEB-99
(SCHEDULED NECROPSY, 18-N0V-98)
(SCHEDULED NECROPSY, 02-DEC-98)
(SCHEDULED NECROPSY, 02-DEC-98)
(SCHEDULED NECROPSY, 02-DEC-98)
267
NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/
-/PFHS/PFOS
'
BACK TO MAIN
MAC-IND -11
25-FEB-99
MACROSCOPIC FINDINGS MALES GROUP 6 (PFOS)
ANIMAL 81
.
NO FINDINGS NOTED
ANIMAL 82
NO FINDINGS NOTED
ANIMAL 83
NO FINDINGS NOTED
ANIMAL 84 NO FINDINGS NOTED
ANIMAL 85
NO FINDINGS NOTED
ANIMAL 86
NO FINDINGS NOTED
ANIMAL 87
NO FINDINGS NOTED
ANIMAL 88 NO FINDINGS NOTED
ANIMAL 89
'
NO FINDINGS NOTED
ANIMAL 90
LIVER..............
ACCENTUATED LOBULAR PATTERN.
ANIMAL 91
NO FINDINGS NOTED
. (SCHEDULED NECROPSY, 03-N0V-98) (SCHEDULED NECROPSY, 03-N0V-98) (SCHEDULED NECROPSY, 03-NOV-98) (SCHEDULED NECROPSY, 03-NOV-98) (SCHEDULED NECROPSY, 04-N0V-98) (SCHEDULED NECROPSY, 04-NOV-9S) (SCHEDULED NECROPSY, 04-N0V-98) (SCHEDULED NECROPSY, 04-NOV-98) (SCHEDULED NECROPSY, 18-N0V-98) (SCHEDULED NECROPSY, 18-N0V-98) (SCHEDULED NECROPSY, 18-NOV-98)
268
NOTOX Project 242933
--
TELOMER ALCOHOL/TELOMER ACRYLATE/^ /PFHS/PFOS
'
BACK TO MAIN
MAC-IND -12 25-FEB-99
MACROSCOPIC FINDINGS MALES GROUP 6 (PFOS) ANIMAL 93
NO FINDINGS NOTED
ANIMAL 94
NO FINDINGS NOTED
ANIMAL 95
NO FINDINGS NOTED
(SCHEDULED NECROPSY, 02-DEC-98) (SCHEDULED NECROPSY, 02-DEC-98) (SCHEDULED NECROPSY, 02-DEC-98)
269
NOTOX Project 242933
--
TELOMER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PFOS
`
BACK TO MAIN
MAC-IND -13 25-FEB-99
MACROSCOPIC FINDINGS FEMALES GROUP 1 (VEHICLE CONTROL)
ANIMAL 97
.
NO FINOINGS NOTED
ANIMAL 98
OVARIES............
LEFT SIDE: WATERY CYST.
ANIMAL 99
NO FINDINGS NOTED
ANIMAL 100
NO FINDINGS NOTED
ANIMAL 101
NO FINDINGS NOTED
ANIMAL 102
NO FINDINGS NOTED
ANIMAL 103
NO FINOINGS NOTED
ANIMAL 104
NO FINDINGS NOTED
ANIMAL 105
SKIN...............
ABDOMINAL REGION: SCAB FORMATION.
ANIMAL 106
NO FINDINGS NOTED
ANIMAL 107
NO FINOINGS NOTED
(SCHEDULED NECROPSY, 05-N0V-98) (SCHEDULED NECROPSY, 05-N0V-98) (SCHEDULED NECROPSY, 05-NOV-98) (SCHEDULED NECROPSY, 05-N0V-9B) (SCHEDULED NECROPSY, 06-N0V-98) (SCHEDULED NECROPSY, 06-N0V-98) (SCHEDULED NECROPSY, 06-N0V-98) (SCHEDULED NECROPSY. 06-NOV-98) (SCHEDULED NECROPSY. 20-NOV-98) (SCHEDULED NECROPSY. 20-N0V-98) (SCHEDULED NECROPSY. 20-N0V-98)
270
NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/
-./PFHS/PFOS
'
BACK TO MAIN
MAC-IND -14 2 5 - F E B -99
MACROSCOPIC FINDINGS FEMALES . GROUP 1 (VEHICLE CONTROL) ANIMAL 109
NO FINDINGS NOTED
ANIMAL 110
NO FINDINGS NOTED
ANIMAL 111
NO FINDINGS NOTED
(SCHEDULED NECROPSY, 04-DEC-98) (SCHEDULED NECROPSY. 04-DEC-98) (SCHEDULED NECROPSY, 04-DEC-9 8 )
271
NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/
-VPFHS/PFOS
*
BACK TO MAIN
MAC-IND -15 25-FEB-99
MACROSCOPIC FINDINGS
FEMALES
.
GROUP 2 (TELOMER ALCOHOL)
ANIMAL 113
NO FINDINGS NOTED
ANIMAL 114
NO FINDINGS NOTED
ANIMAL 115
NO FINDINGS NOTED
ANIMAL 116
NO FINDINGS NOTED
ANIMAL 117
KIDNEYS...........................
RIGHT SIDE: PELVIC DILATION.
ANIMAL 118
NO FINDINGS NOTED
ANIMAL 119
NO FINDINGS NOTED
ANIMAL 120
NO FINDINGS NOTED
ANIMAL 121
NO FINDINGS NOTED
ANIMAL 122
SKIN.................................. ALOPECIA.
ANIMAL 123
NO FINDINGS NOTED
* (SCHEDULED NECROPSY, 05-N0V-98) . (SCHEDULED NECROPSY, 05-N0V-98) (SCHEDULED NECROPSY, 0 5 -NOV-9 8 ) (SCHEDULED NECROPSY, 0 5 -NOV-98) (SCHEDULED NECROPSY, 06-N0V-98) (SCHEDULED NECROPSY,' 06-NOV-98) (SCHEDULED NECROPSY, 0 6 -NOV-9 8 ) (SCHEDULED NECROPSY, 06-NOV-98) (SCHEDULED NECROPSY. 20-N0V-98) (SCHEDULED NECROPSY, 20-N0V-98) (SCHEDULED NECROPSY, 2 0 -NOV-98)
272
NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/
-!PFHS/PFOS
'
BACK TO MAIN
MAC-IND -16 25-FEB-99
MACROSCOPIC FINDINGS
FEMALES
.
GROUP 2 (TELOMER ALCOHOL)
ANIMAL 125
NO FINDINGS NOTED
ANIMAL 126
NO FINDINGS NOTED
ANIMAL 127
NO FINDINGS NOTED
(SCHEDULED NECROPSY, 04-DEC-98) (SCHEDULED NECROPSY, 04-DEC-98) . (SCHEDULED NECROPSY, 04-DEC-98)
273
NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/
-_ 7 PFHS/PFOS
MACROSCOPIC FINDINGS FEMALES GROUP 3 (TELOMER ACRYLATE) ANIMAL 129 NO FINDINGS NOTED ANIMAL 130 NO FINDINGS NOTED ANIMAL 131 NO FINDINGS NOTED ANIMAL 132 NO FINDINGS NOTED ANIMAL 133 NO FINDINGS NOTED ANIMAL 134 NO FINDINGS NOTED ANIMAL 135 NO FINDINGS NOTED ANIMAL 136 NO FINDINGS NOTED ANIMAL 137 NO FINDINGS NOTED ANIMAL 138 NO FINDINGS NOTED ANIMAL 139 NO FINDINGS NOTED
BACK TO MAIN
MAU-1INU -1/ 2 5 - F E B -99
(OTHER, 05-N0V-98) (SCHEDULED NECROPSY. 05-N0V-98) (SCHEDULED NECROPSY, 05-N0V-98) (SCHEDULED NECROPSY, 05-N0V-98) (SCHEDULED NECROPSY, 06-N0V-98) (SCHEDULED NECROPSY. 06-N0V-98) (SCHEDULED NECROPSY, 06-NOV-9 8 ) (SCHEDULED NECROPSY. 06-N0V-98) (SCHEDULED NECROPSY, 20-N0V-98) (SCHEDULED NECROPSY, 20-N0V-98) (SCHEDULED NECROPSY, 20-NOV-98)
274
NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/
/PFHS/PFOS
BACK TO MAIN
MAC-IND -18 25-FEB-99
MACROSCOPIC FINDINGS FEMALES GROUP 3 (TELOMER ACRYLATE) ANIMAL 141
NO FINDINGS NOTED
ANIMAL 142
NO FINDINGS NOTED
ANIMAL 143
NO FINDINGS NOTED
* (SCHEDULED NECROPSY, 04-DEC-98)
(SCHEDULED NECROPSY, 04-DEC-98)
(SCHEDULED NECROPSY, 04-DEC-98)
275
NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/
/PFHS/PFOS
MACROSCOPIC FINDINGS
FEMALES
GROUP 4 (
)
ANIMAL 145
NO FINDINGS NOTED
ANIMAL 146
NO FINDINGS NOTED
ANIMAL 147
NO FINDINGS NOTED
ANIMAL 148
NO FINDINGS NOTED
ANIMAL 149
NO FINDINGS NOTED
ANIMAL 150
NO FINDINGS NOTED
ANIMAL 151
UTERUS.............................
LEFT SIDE: CYST(S).
ANIMAL 152 NO FINOINGS NOTED
ANIMAL 153
NO FINDINGS NOTED
ANIMAL 154
NO FINDINGS NOTED
ANIMAL 155
NO FINDINGS NOTED
BACK TO MAIN
` MAC-IND -19 25-FEB-99
'* (SCHEDULED NECROPSY, 05-N0V-98 )
(SCHEDULED NECROPSY, 05-NOV-9 8 )
(SCHEDULED NECROPSY, 05-N0V-98)
(SCHEDULED NECROPSY, 05-NOV-98)
(SCHEDULED NECROPSY, 06-NOV-9 8 )
(SCHEDULED NECROPSY, 06-NOV-98)
(SCHEDULED NECROPSY, 0 6 -NOV-98)
(SCHEDULED NECROPSY, 06-NOV-98) (SCHEDULED NECROPSY, 20-NOV-98)
(SCHEDULED NECROPSY, 2 0 -NOV-98)
(SCHEDULED NECROPSY, 20-N0V-98)
276
NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/
-,, /PFHS/PFOS
BACK TO MAIN
MAC-IND -20 25-FEB-99
MACROSCOPIC FINDINGS
FEMALES
GROUP 4 (
)
ANIMAL 157
NO FINDINGS NOTED
ANIMAL 158
NO FINDINGS NOTED
ANIMAL 159
NO FINDINGS NOTED
(SCHEDULED NECROPSY, 04-DEC-98) (SCHEDULED NECROPSY, 04-DEC-98) (SCHEDULED NECROPSY, 04-DEC-98)
277
NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/
.^ /PFHS/PFOS
BACK TO MAIN
MAC-IND -21 25-FEB-99
MACROSCOPIC FINDINGS FEMALES GROUP 5 (PFHS) ANIMAL 161 NO FINDINGS NOTED ANIMAL 162 NO FINDINGS NOTED ANIMAL 163 NO FINDINGS NOTED ANIMAL 164 NO FINDINGS NOTED ANIMAL 165 NO FINDINGS NOTED ANIMAL 166 NO FINDINGS NOTED ANIMAL 167 NO FINDINGS NOTED ANIMAL 168 NO FINDINGS NOTED ANIMAL 169 NO FINDINGS NOTED ANIMAL 170 NO FINDINGS NOTED ANIMAL 171 NO FINDINGS NOTED
* (SCHEDULED NECROPSY, 05-N0V-98) (SCHEDULED NECROPSY, 05-N0V-98) (SCHEDULED NECROPSY, 05-N0V-98) (SCHEDULED NECROPSY, 0 5 -NOV-9 8 ) (SCHEDULED NECROPSY. 06-NOV-98) (SCHEDULED NECROPSY, 06-NOV-98) (SCHEDULED NECROPSY, 06-NOV-98) (SCHEDULED NECROPSY, 06-NOV-98) (SCHEDULED NECROPSY, 20-N0V-98) (SCHEDULED NECROPSY, 20-N0V-98) (SCHEDULED NECROPSY, 20-N0V-98)
278
NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/
-/PFHS/PFOS
'
BACK TO MAIN
MAC-IND-22 25-FEB-99
MACROSCOPIC FINDINGS FEMALES GROUP 5 (PFHS) ANIMAL 173
NO FINDINGS NOTED
ANIMAL 174
NO FINDINGS NOTED
ANIMAL 175
NO FINDINGS NOTED
(SCHEDULED NECROPSY, 04-DEC-98) (SCHEDULED NECROPSY, 04-DEC-98) (SCHEDULED NECROPSY, 04-DEC-98)
279
NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/
'' 'PFHS/PFOS
"
BACK TO MAIN
MAC-IND -23 25-FEB-99
MACROSCOPIC FINDINGS FEMALES GROUP 6 (PFOS)
ANIMAL 177
NO FINDINGS NOTED
ANIMAL 178
.
NO FINDINGS NOTED
ANIMAL 179
.
NO FINDINGS NOTED ANIMAL 180
NO FINDINGS NOTED
ANIMAL 181
NO FINDINGS NOTED
ANIMAL 182
NO FINDINGS NOTED
ANIMAL 183
.
NO FINDINGS NOTED
ANIMAL 184 NO FINDINGS NOTED
ANIMAL 185
NO FINDINGS NOTED
ANIMAL 186
NO FINDINGS NOTED
ANIMAL 187
NO FINDINGS NOTED
.
(SCHEDULED NECROPSY, 05-N0V-98) (SCHEDULED NECROPSY, 05-N0V-98) (SCHEDULED NECROPSY, 05-N0V-98) (SCHEDULED NECROPSY, 05-N0V-98) (SCHEDULED NECROPSY, 06-N0V-98) (SCHEDULED NECROPSY, 06-N0V-98) (SCHEDULED NECROPSY, 06-N0V-98) (SCHEDULED NECROPSY, 06-NOV-98) (SCHEDULED NECROPSY, 20-N0V-98) (SCHEDULED NECROPSY, 20-N0V-98) (SCHEDULED NECROPSY, 20-N0V-98)
* 280
NOTOX Project 242933 TELOMER ALCOHOL/TELOMER ACRYLATE/
-/PFHS/PFOS
`'
BACK TO MAIN
MAC-IND -24 25-FEB-99
MACROSCOPIC FINDINGS FEMALES GROUP 6 (PFOS) ANIMAL 189
NO FINDINGS NOTED
ANIMAL 190
NO FINDINGS NOTED
ANIMAL 191
NO FINDINGS NOTED
.
(SCHEDULED NECROPSY, 0 4 -DEC-98) (SCHEDULED NECROPSY, 04-DEC-98) (SCHEDULED NECROPSY. 0 4 -DEC-98)
281
BACK TO MAIN
NOTOX Project 242933
ORGAN WEIGHTS (GRAM) AFTER 4 WEEKS MALES
GROUP 1 (VEHICLE CONTROL)
ANIMAL NUMBER
BODY W.
BRAIN
HEART
1 284 2 332
3 348 4 270 5 303 6 327 7 351 8 301
1.95 2.04 2.03 2.00
1.92 2.01 2.14
1.99
1.081 1.390 1.108 1.070 1.141 1.088 1.123
1.059
LIVER
8.04 10.76 10.40
8.54 10.49 10.38 11.92
9.84
OW-IND - X 30-MAR-99
KIDNEYS ADRENALS SPLEEN
TESTES
1.84 2.59 2.41
1.75 2.54 2.06 2.63 2.59
0.038 0.050 0.037
0.040 0.053 0.044
0.051 0.047
0.434 0.565 0.563 0.482
0.488 0.733 0.766 0.605
2.71 2.79 3.32
2.53 2.91 2.82
3.03 2.82
GROUP 2 (TELOMER ALCOHOL)
ANIMAL NUMBER
BODY W.
BRAIN
HEART
17 322
18 295 19 312 20 299 21 315 22 348 23 313 24 312
1.99 2.01
2.00 1.96 1.84 2.05 1.87 1.95
1.149 1.300
0.935 1.033 1.129 1.309 0.972 1.241
LIVER
10.16 11.71
9.71 9.50 10.48 10.71 10.15 10.42
KIDNEYS ADRENALS SPLEEN
TESTES
2.35 2.57 2.35 2.24 2.40 2.57 2.12
2.09
0.049 0.050 0.053 0.055 0.050 0.049 0.072 0.049
0.465 0.551 0.541 0.540 0.565 0.712 0.560 0.590
3.05 3.27
3.19 3.21 2.63 3.18 2.87 2.96
GROUP 3 (TELOMER ACRYLATE)
ANIMAL NUMBER
BODY W.
BRAIN
HEART
33 313
1.97
34 288
1.93
35 293
1.95
36 293
1.94
37 303
2.02
38 324
1.98
39 319 - 1.99
40 304
1.82
1.127 0.943
1.131 0.972 1 .3 2 1 1.079 1.077 1.122
LIVER
9.89 8.88 8.71 8.90 9.66 10.40 9.48 8.95
KIDNEYS ADRENALS SPLEEN
TESTES
2.08 2.16 2.08 2.14 2.50
2.37 2.19 2.12
0.053 0.046 0.038 0.059 0.058 0.056 0.046 0.056
0.637
0.498 0.476 0.530 0.603 0.592 0.656 0.547
2.52 2.77 2.77 2.74 2.84 2.97 2.69 2.94
GROUP 4 ( )
ANIMAL BODY W. BRAIN NUMBER
49 290 50 296 51 342 52 324 53 320 54 330 55 284 56 263
1.96 2.07 2 .11 1.97 1.98
1.99 1.97
1.93
HEART
1.055 1.193 1.156 1.224 1.331 1.207 1.025 1.043
LIVER
8.23 9.16 10.83 9.52 9.39 9.53 8.50 7.18
KIDNEYS ADRENALS SPLEEN TESTES
1.99 2.08 2.54 2.26 2.36 2.25 2.07 1.73
0.046 0.033 0.046 0.053 0.042 0.041 0.045
0.058
0.488 0.627 0.719 0.559 0.629 0.547 0.537
0.519
3.18 3.00 2.77 2.96 2.58 2.70 2.95 2.75
282
BACK TO MAIN
NOTOX Project 242933
ORGAN WEIGHTS (GRAM) AFTER 4 WEEKS MALES
GROUP 5 (PFHS)
ANIMAL NUMBER
BODY W.
BRAIN
HEART
65 66 67 68
69 70 . 71
72
352
270 297 297 287 347
311 306
2.02
1.89 2.03 1.94 1.86 2.07 1.83 2.04
1.267
0.992 1.131 1.148 1.096 1.153 1.030 1.040
GROUP 6 (PFOS)
ANIMAL NUMBER
BODY W.
BRAIN
81 303 82 295 83 308 84 318 85 362 86 293 87 351 88 317
1.99 1.98 2.06 2.00
1.96 1.93 1.97 2.07
HEART
1.102 1.165 0.961 1.171 1.223 1.259 1.163 1.004
LIVER
16.85 11.52 13.86 13.10 12.96 14.49 11.84 15.62
LIVER
11.04 11.17 10.67 11.52 13.46 12.90 12.33 11.19
OW-IND - 2 30-MAR-9 9
KIDNEYS ADRENALS SPLEEN
TESTES
2.77 2.14
2.49 2.31 2.11 2.47 2.19
2.28
0.037 0.038 0.059 0.046 0.058 0.057 0.051 0.056
0.595 0.504 0.549 0.541
0.570 0.745 0.516 0.539
3.22 0.85 3.18 3.07
2.82 3.18 3.05 3.04
KIDNEYS ADRENALS SPLEEN
TESTES
2.15 2.21
1.91 2.42 2.34 2.28 2.26 1.96
0.039 0.055
0.039 0.032 0.060 0.039 0.067 0.040
0.501 0.433 0.560 0.510
0.692 0.664 0.634 0.469
2.80 2.86
2.82 2.79 2.88
2.58 3.22
2.84
283
BACK TO MAIN
NOTOX Project 242933
ORGAN/BODY WEIGHT RATIOS {%) AFTER 4 WEEKS HALES
GROUP 1 (VEHICLE CONTROL)
ANIMAL NUMBER
BODY W. (GRAM)
BRAIN
HEART
LIVER
KIDNEYS ADRENALS SPLEEN
1 284 2 332 3 348 4 270 5 303 6 327
"7 351 8 301
0.69 0.61 0.58 0.74
0.63 0.62
0.61 0.66
0.381 0.419 0.318 0.396 0.377 0.333 0.320
0.352
2.83 3.24 2.99
3.16 3.46 3.18 3.40 3.27
0.65 0.78 0.69 0.65
0.84 0.63
0.75 0.86
0.013
0.015 0.011 0.015 0.017 0.013 0.015 0.016
0.153 0.170 0.162 0.179
0.161 0.224 0.218 0.201
OW-IND - 3 3 0 - M A R - 99
TESTES
0.95 0.84 0.95 0.94 0.96 0.86 0.86 0.94
GROUP 2 (TELOMER ALCOHOL)
ANIMAL NUMBER
BODY W. (GRAM)
BRAIN
HEART
17 322 18 295 19 312 20 299 21 315 22 348 23 313 24 312
0.62 0.68
0.64
0.65 0.58 0.59 0.60 0.62
0.357
0.441 0.300 0.345 0.358 0.376 0.311
0.398
LIVER
3.15 3.97 3.11 3.18 3.33 3.08 3.24 3.34
KIDNEYS ADRENALS SPLEEN
TESTES
0.73 0.87 0.75 0.75 0.76 0.74 0.68
0.67
0.015 0.017 0.017
0.018 0.016 0.014 0.023 0.016
0.144 0.187 0.173
0.181 0.179 0.205 0.179
0.189
0.95 1 .1 1 1.02
1.07 0.83 0.91
0.92 0.95
.
.
GROUP 3 (TELOMER ACRYLATE)
ANIMAL NUMBER
BODY W. (GRAM)
BRAIN
HEART
33 313 34 288 35 293 36 293 37 303 38 324 39 319 40 304
0.63 0.67 0.66
0.66 0.67 0.61 0.62 0.60
0.360 0.327 0.386 0.332 0.436 0.333 0.338 0.369
LIVER
3.16 3.08 2.97 3.04 3 .1 9 3.21 2.97 2.94
KIDNEYS ADRENALS SPLEEN
TESTES
0.66
0.75 0.71 0.73 0.83 0.73 0.68 0.70
0.017 0.016 0.013 0.020 0.019
0.017 0.014 0.018
0.204 0.173 0.162 0.181 0.199 0.183 0.206 0.180
0.80 0.96 0.95 0.94 0.94 0.92 0.84 0.97
GROUP 4 ( )
ANIMAL BODY W. BRAIN NUMBER (GRAM)
49 290 50 296 51 342 52 324 53 320 54 330
55 284 56 263
0.67 0.70 0.62
0.61 0.62 0.60 0.69 0.73
HEART
0.364 0.403 0.338 0.378 0.416 0.366 0.361 0.397
LIVER
2.84 3.09 3.17 2.94 2.93 2.89 2.99 2.73
KIDNEYS ADRENALS- SPLEEN TESTES
0.69 0.70 0.74 0.70
0.74 0.68
0.73 0.66
0.016 0.011 0.013 0.016 0.013 0.012
0.016 0.022
0.168 0.212 0.210
0.173 0.197
0.166 0.189 0.197
1.10 1.0 1
0.81 0.91 0.81 0.82 1.04 1.05
284
BACK TO MAIN
NOTOX Project 242933
ORGAN/BODY WEIGHT RATIOS (%) AFTER 4 WEEKS MALES
GROUP 5 (PFHS)
ANIMAL NUMBER
BODY W. (GRAM)
BRAIN
HEART
LIVER
65 352 66 270 67 297 68 297 69 287 70 347 71 311 72 306
0.57 0.70 0.68
0.65 0.65
0.60 0.59 0.67
0.360 0.367 0.381 0.387 0.382 0.332 0.331 0.340
4.79 4.27 4.67
4.41 4.52 4.18 3.81 5.10
OW-IND - 4 3 0 - M A R - 99
KIDNEYS ADRENALS SPLEEN
TESTES
0.79 0.79 0.84 0.78 0.73 0.71
0.70 0.74
0.011
0.014 0.020 0.015
0.020 0.016 0.016 0.018
0.169 0.187 0.185 0.182 0.199 0.215 0.166 0.176
0.91 0.31 1.07 1.03
0.98 0.92 0.98 0.99
GROUP 6 (PFOS)
ANIMAL NUMBER
BODY W. (GRAM)
BRAIN
HEART
81 303 82 295 83 308 84 318
85 362 86 293 87 351 88 317
0.66
0.67 0.67
0.63 0.54 0.66
0.56 0.65
0.364
0.395 0.312 0.368 0.338
0.430 . 0.331
0.317
LIVER
3.64 3.79 3.46 3.62 3.72 4.40 3.51 3.53
KIDNEYS ADRENALS SPLEEN
TESTES
0.71
0.75 0.62 0.76
0.65 0.78 0.64
0.62
0.013 0.019 ' 0.013 0.010
0.017
0.013 0.019 0.013
0.165 0.147 0.182 0.160 0.191 0.227
0.181 0.148
0.92 0.97 0.91 0.88 0.80 0.88 0.92 0.90
BACK TO MAIN
NOTOX Project 242933
ORGAN WEIGHTS (GRAM) AFTER 4 WEEKS FEMALES
GROUP 1 (VEHICLE CONTROL)
ANIMAL NUMBER
BODY W.
BRAIN
HEART
97 201 98 194 99 204 100 217 101 241 102 229 103 183 104 242
1.93 1.92 1.89 1.96
1.91 1.89 1.70 1.85
0.861 0.799 0.750
0.869 0.917
0.901 0.855 0.867
LIVER
6.95 6.34 6.13 6.82 7.19 6.61 5.79 7.13
OW-IND - 5 3 0 - M A R - 99
KIDNEYS ADRENALS SPLEEN
OVARIES
1.44
1.39 1.35 1.56 1.71 1.49 1.36 1.71
0.060 0.075 0.052
0.057 0.046 0.065 0.062 0.061
0.424
0.345 0.398 0.483 0.458 0.366 0.426 0.481
0.089 0.142 0.128 0.119 0.130 0.163 0.100 0.122
GROUP 2 (TELOMER ALCOHOL)
ANIMAL NUMBER
BODY W.
BRAIN
HEART
113 204
114 246 115 215
116 218 117 194 118 200 119 201 120 213
1.91 2.03 1.92 2.08 1.82 1.89 1.93 1.88
0.890 0.883 0.785 0.827 0.749 0.829 0.751
0.786
LIVER
6.96 8.13 7.39 7.59 6.97 6.78 6.35 7.29
KIDNEYS ADRENALS SPLEEN
OVARIES
1.47
1.85
1.75 1.44 1.68 1.59 1.48 1.75
0.055 0.056
0.078 0.062 0.073 0.054 0.067
0.069
.470
0.479 0.501 0.378 0.433 0.435 0.446 0.457
0.129 0.130 0.123 0.121 0.172 0.144 0.104 0.142
GROUP 3 (TELOMER ACRYLATE)
ANIMAL NUMBER
BODY W.
BRAIN
HEART
129 204
___
130 182
1.84
131 196
1.86
132 205 ' 1.88
133 1S1
1.77
134 215
1.92
135 227
1.85
136 193
1.79
___
0.744 0.736 0.847 0.770 0.799 0.917 0.714
LIVER
--
5.70 6.48 6.23 6.19 6.84 7.07 6.09
KIDNEYS ADRENALS SPLEEN
OVARIES
...
1.42 1.61 1.59 1.36 1.57 1.62 1.41
- -
0.052 0.053 0.060 0.054 0.063 0.077 0.060
----
0.298 0.300 0.479 0.454 0.494 0.4870.409
----
0.127 0.162 0.138 0.125 0.127 0.146 0.118
GROUP 4 ( )
ANIMAL NUMBER
BODY W.
BRAIN
145 193 146 222
147 223 148 186 149 220 150 219 151 200 152 201
1.80
1.89 2.01 1.84 1.96 2.00
1.84 1.85
HEART
0.794 0.961 0.794 0.767 0.855 0.773 0.778 0.719
LIVER
6.44 6.76 6.05 5.72 8.10 6.71 6.22 6.28
KIDNEYS ADRENALS SPLEEN
OVARIES
1.53
1.46 1.64 1.50 1.89 1.48 1.41 1.33
0.066 0.160 0.057 0.043 0.055 0.068 0.049
0.061
1.527 0.529 0.511 0.506
0.420 0.478 0.463 0.429
0.131 0.055 0.138 0.116 0.156
0.130 0.136 0.129
286
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NOTOX Project 242933
ORGAN WEIGHTS (GRAM) AFTER 4 WEEKS FEMALES
GROUP 5 (PFHS)
ANIMAL NUMBER
BODY W.
BRAIN
HEART
iei 193 162 201 163 191 164 202 165 198
166 208 167 183 168 228
1.94 1.93 1.78 1.78 1.81 1.98 1.86
1.99
0.769 0.703 0.816 0.800
0.910 0.881 0.688 0.880
GROUP 6 (PFOS)
ANIMAL NUMBER
BODY W.
BRAIN
177 215 178 163 179 185 180 173 181 185 182 199 183 191 184 188
1.86
1.79 1.91 1.86 1.88
1.96 1.91 1.88
HEART
0.788 0.660 0.663 0.673 0.818 0.845 0.777 0.757
LIVER
7.96 6.70 5.97 6.57 7.08 6.67 5.68 7.08
LIVER
9.28 6.47 7.23 7.16 7.62 8.41 7.85 8.36
OW-IND - 6 30-MAR-99
KIDNEYS ADRENALS SPLEEN
OVARIES
1.61 1.42 1.32 1.68
1.46 1.48 1.47 1.62
0.068 0.056 0.060 0.047 0.071 0.062 0.049 0.052
0.436 0.419 0.397
0.443 0.361 0.431 0.357 0.415
0.150 0.102 0.121
0.138 0.138 0.152 0.115 0.141
KIDNEYS ADRENALS SPLEEN
OVARIES
1.69 1.29 1.48 1.41 1.39 1.47
1.43 1.52
0.053 0.044 0.047
0.061 0.055 0.059 0.059 0.057
0.474 0.327 0.386
0.394 0.404 0.420 0.442 0.450
0 .111
0.135 0.108 0.115 0.146 0.110
0.154 0.148
287
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NOTOX Project 242933
ORGAN/BODY WEIGHT RATIOS (%) AFTER 4 WEEKS FEMALES
GROUP 1 (VEHICLE CONTROL)
ANIMAL NUMBER
BODY W. (GRAM)
BRAIN
HEART
LIVER
97 201
98 194 99 204 100 217 101 241 102 229 103 183 104 242
0.96
0.99 0.93 0.90 0.79 0.83 0.93 0.76
0.428 0.412
0.368 0.400 0.380 0.393 0.467
0.358
3.46 3.27
3.01 3.14
2.98 2.89 3.16 2.95
OW-IND - 7 3 O-MAR-9 9
KIDNEYS ADRENALS SPLEEN
OVARIES
0.71 0.71
0.66 0.72 0.71 0.65 0.74 0.71
0.030 0.039
0.025 0.026 0.019 0.028 0.034 0.025
0.211
0.178 0.195 0.223 0.190 0.160 0.233 0.199
0.044 0.073 0.063 0.055 0.054 0.071
0.055 0.050
GROUP 2 (TELOMER ALCOHOL)
ANIMAL NUMBER
BODY W. (GRAM)
BRAIN
HEART
113 204 114 246 115 215 116 218 117 194 118 200 119 201 120 213
0.94
0.83 0.89 0.95 0.94 0.95 0.96 0.88
0.436
0.359 0.365 0.379 0.386 0.415 0.374 0.369
LIVER
3.41 3.31 3.44 3.48 3.59 3.39 3.16 3.42
KIDNEYS ADRENALS SPLEEN
OVARIES
0.72 0.75 0.81 0.66
0.87 0.80 0.73 0.82
0.027 0.023 0.036 0.028 . 0.038 0.027
0.033 0.032
0.230 0.195 0.233 0.173 0.223 0.218 0.222
0.215
0.063 0.053 0.057
0.056 0.089 0.072 0.052 0.067
GROUP 3 (TELOMER ACRYLATE)
ANIMAL NUMBER
BODY W. (GRAM)
BRAIN
HEART
129 204 130 182 131 196 132 205 133 181 134 215 135 227 136 193
1.0 1
0.95 0.92
0 .9 8
0.89 0.81 0.93
0.409 0.376 0.413
0 .425
0.372 0.404 0.370
LIVER
3.13 3.31 3.04
3 .4 2
3.18 3.11 3.16
KIDNEYS ADRENALS SPLEEN
OVARIES
0.78 0.82 0.78
0 .75
0.73 0.71
0.73
0.029 0.027
0 .0 2 9
0 .0 3 0
0.029 0.034 0.031
0.164 0.153
0 .2 3 4
0.251 0.230 0.215 0.212
0.070 0.083
0 .0 67
0.069 0.059 0.064 0.061
GROUP 4 ( )
ANIMAL NUMBER
BODY W. (GRAM)
BRAIN
145 193 146 222 147 223 148 186 149 220 150 219 151 200 152 201
0.93 0.85 0.90
0.99 0.89 0.91 0.92 0.92
HEART
0.411 0.433 0.356 0.412 0.389 0.353 0.389 0.358
LIVER
3.34 3.04 2.71 3.07 3.68 3.06 3.11 3.12
KIDNEYS ADRENALS SPLEEN
OVARIES
0.79 0.66
0.73 0.80 0.86 0.68
0.70 0.66
0.034 0.072 0.026 0.023 0.025 0.031
0.025 0.030
0.791 0.238 0.229 0.272 0.191 0.218
0.232 0.213
0.068 0.025 0.062 0.062 0.071 0.059 0.068 0.064
288
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NOTOX Project 242933
ORGAN/BODY WEIGHT RATIOS (%) AFTER 4 WEEKS FEMALES
GROUP 5 (PFHS)
an im al
NUMBER
BODY W. (GRAM)
BRAIN
HEART
LIVER
16 1 193 16 2 201 163 191 1 6 4 202 165 198 16 6 208 16 7 183 168 228
1.01 0 .96 0 .9 3 0.88 0 .92
0 .95 1.01 0 .87
0 .3 9 8 0 .350 0 .42 7 0 .396 0 .4 6 0 0 .42 4 0 .3 76 0 .386
4 .13 3 .33 3 .13 3 .2 5 3 .5 7 3 .2 1 3 .10 3 .10
GROUP 6 (PFOS)
ANIMAL NUMBER
BODY W. (GRAM)
BRAIN
177 178 179 180 . 181 182 183 184
215 163 185 173 185 199 191 188
0.86 1.10 1.0 3 1.0 7 1.02 0 .9 9 1.00 1.00
HEART
0 .3 67 0.40 5 0 .358 0 .389 0 .442 0 .42 5 0 .40 7 0 .4 0 3
LIVER
4.3 2 3 .9 7 3 .9 1 4 .14 4 .12 4 .2 3 4 .11 4 .4 5
O W -IN D - 8 3 0-M A R -9 9
KIDNEYS
ADRENALS SPLEEN
OVARIES
0 .8 3 0 .70 0 .69 0 .8 3 0 .74 0 .71 0 .8 0 0 .71
0 .0 3 5 0 .0 2 8 0 .0 31 0 .0 2 3 0 .0 3 6 0 .0 3 0 0.0 27 0 .0 2 3
0 .226 0 .2 0 8 0 .2 0 8 0 .219 0 .182 0.20 7 0 .19 5 0 .182
0 .0 7 8 0 .0 51 0 .0 63 0 .0 6 8 0.0 70 0.0 73 0 .0 6 3 0 .0 6 2
.
KIDNEYS
ADRENALS SPLEEN
OVARIES
0 .79 0 .79 0 .8 0 0 .8 2 0 .75 0 .74 0 .75 0 .8 1
0 .0 2 5 0 .0 2 7 0 .0 2 5 0 .0 3 5 0 .0 3 0 0 .0 3 0 0 .0 31 0 .0 3 0
0.220 0.201 0 .2 0 9 0 .228 0 .218 0.211 0 .2 3 1 0 .239
0 .0 52 0 .0 8 3 0.0 58 0 .0 66 0 .0 79
0 .0 55 0 .0 8 1 0 .0 79
28 9
BACK TO MAIN
NOTOX Project 242933
ORGAN WEIGHTS (GRAM) AFTER 2 WEEKS RECOVERY MALES
GROUP 1 (VEHICLE CONTROL)
ANIMAL NUMBER
BOOT W.
BRAIN
HEART
9 344 10 277 11 338
2.0 3 1.8 1 2.01
1.18 5 0 .960 1.12 8
LIVER
8 .98 7.4 2 8 .32
O W -IN D - 9 3 0 -M A R -99
KIDNEYS
ADRENALS SPLEEN
TESTES
2.02 1.7 4 2 .3 5
0 .0 3 9 0 .0 37 0 .0 5 0
0 .440 0 .358 0 .526
2.88 2 .5 5 3 .0 1
GROUP 2 (TELOMER ALCOHOL)
ANIMAL NUMBER
BOOT W.
BRAIN
HEART
25 328 26 295 27 320
1.9 3 1.9 6 2.0 5
1.0 0 7 1.0 68 1.112
LIVER
8 .62 7.5 4 8 .65
KIDNEYS
ADRENALS SPLEEN
2 .0 4 2.11 2 .0 9
0 .0 41 0 .046 0 .0 31
0 .578 0 .5 71 0 .555
2 .6 7 2 .6 2 1.10
GROUP 3 (TELOMER ACRYLATE)
ANIMAL NUMBER
BOOT W.
BRAIN
HEART
LIVER
41 379
42 319 43 334
2.01 2 .0 4 1.8 0
1 .1 1 3 1.2 8 4 1 .119
12.10 8.88 8 .7 1
GROUP 4 (
\
ANIMAL NUMBER
BOOT W.
BRAIN
57 4 15 ' 58 354 59 329
2 .13 2.02
1 .9 1
HEART
1.12 5 1.17 9 1.15 5
LIVER
11 .4 1 10 .4 5
9 .0 5
KIDNEYS
ADRENALS SPLEEN
TESTES
2 .75 2 .15 2 .3 3
0.0 47 0 .0 36 0.0 47
0 .5 0 4 0 .578 0 .547
3 .16 3 .19 2 .9 1
KIDNEYS
ADRENALS SPLEEN
TESTES
2 .56 2 .5 0
2.11
0 .0 41 0 .0 6 3
0 .0 6 2
0 .739 0 .582
0 .548
3 .53 3 .3 5
3 .18
GROUP 5 (PFHS)
ANIMAL NUMBER
BOOT W.
BRAIN
73 386 74 249 75 336
2.00 1.7 3 2 .0 6
HEART
1.3 0 9 1.0 2 7 1.112
LIVER
12 .15 6 .8 9 9 .6 2
KIDNEYS
ADRENALS s p l e e n
TESTES
2 .54 1.6 0 2 .4 1
0 .0 4 5 0 .0 3 5 0 .0 4 3
0 .6 3 0 0 .476 0 .5 17
3 .2 7 1.3 4 3 .2 1
290
BACK TO MAIN
NOTOX Project 242933
ORGAN WEIGHTS (GRAM) AFTER 2 WEEKS RECOVERY MALES
GROUP 6 (PFOS)
ANIMAL NUMBER
BODY W.
BRAIN
HEART
89 394 90 370 91 377
2 .03 2 .0 7 2.00
1.3 3 3 1.2 3 7 1.3 2 8
O W - I N D - 10 3 0 - M A R - 99
*
LIVER
KIDNEYS
ADRENALS SPLEEN
TESTES
14 .2 0
2.66
14 .6 2 2 .58
13 .3 9
2 .70
0 .0 41 0 .0 4 8 0 .0 54
0 .758 0 .6 3 1 0 .5 17
3 .3 1 2 .8 0 2 .9 7
291
BACK TO MAIN
NOTOX Project 242933
ORGAN/BODY WEIGHT RATIOS (%) AFTER 2 WEEKS RECOVERY MALES
GROUP 1 (VEHICLE CONTROL)
ANIMAL NUMBER
BODY W. (GRAM)
BRAIN
REART
LIVER
9 344 10 2 77
11 338
0 .59 0 .65 0 .6 0
0 .344 0 .347 0 .334
2 .6 1 2.68 2 .4 6 .
GROUP 2 (TELOMER ALCOHOL)
ANIMAL NUMBER
BODY W. (GRAM)
BRAIN
HEART
25 328 26 295 27 320
0 .59 0 .67
0 .64
0 .30 7 0 .362 0 .348
LIVER
2 .63 2 .5 6 2 .70
GROUP 3 (TELOMER ACRYLATE)
ANIMAL NUMBER
BODY W. (GRAM)
BRAIN
HEART
LIVER
41 379 42 319 43 334
0 .53 0 .64 0 .54
0 .294 0 .4 0 3 0 .335
3 .19 2 .78 2 .6 1
GROUP 4 ( )
ANIMAL NUMBER
BODY W. (GRAM)
BRAIN
57 415' 58 354
59 329
0 .5 1 0 .57
0 .58
HEART
0 .2 71 0 .333 0 .3 51
LIVER
2 .7 5 2 .9 5 2 .75
GROUP 5 (PFHS)
ANIMAL NUMBER
BODY H. (GRAM)
BRAIN
73 386 74 249 75 336
0 .52 0 .6 9 0 .6 1
HEART
0 .339 0 .412 0 .3 3 1
LIVER
3 .15 2 .7 7 2.86
O W - I N D - 11 3 O-MAR-99
*
KIDNEYS
ADRENALS SPLEEN
TESTES
0 .59 0 .63 0 .70
0.011 0.0 13 0 .0 15
0 .128 0 .129 0 .156
0 .8 4 0 .92 0 .8 9
KIDNEYS
ADRENALS SPLEEN
TESTES
0 .62 0 .72 0 .65
0 .0 13 0 .0 16 0.010
0 .176 0 .194 0 .173
0 .8 1 0 .89 0 .3 4
KIDNEYS
ADRENALS s p l e e n
TESTES
0 .73 0 .6 7 0 .70
0.012 0.011 0 .0 14
0 .133 0 .18 1 0 .164
0 .8 3 1.00 0 .8 7
>
KIDNEYS
ADRENALS SPLEEN
TESTES
0 .62 0 .7 1
0 .64
0.010 0 .0 18
0 .0 19
0 .178 0 .164
0 .16 7
0 .8 5 0 .95
0 .9 7
KIDNEYS
ADRENALS SPLEEN
TESTES
0.66 0 .64 0 .72
0.012 0 .0 14 0 .013
0 .16 3 . 0 .8 5
0 .19 1
0 .54
0 .154
0 .95
292
BACK TO MAIN
NOTOX Project 242933
ORGAN/BODY WEIGHT RATIOS (%) AFTER 2 WEEKS RECOVERY HALES
GROUP' 6 (PFOS)
ANIMAL NUMBER
BOOT W. (GRAM)
BRAIN
HEART
LXVER
89 394 90 370 91 377
0 .52 0 .5 6 0 .53
0 .338 0 .334 0 .352
3 .6 0 3 .9 5 3 .5 5
O W - I N D - 12 3 0 - M A R - 99
*
KIDNEYS
ADRENALS s p l e e n
TESTES
0.68 0 .70 0 .72
0.010 0 .0 13 0 .0 14
0 .192 0 .17 1 0 .13 7
0 .8 4 0 .76 0 .79
293
BACK TO MAIN
NOTOX Project 242933
ORGAN WEIGHTS (GRAM) AFTER 2 WEEKS RECOVERY FEMALES
GROUP 1 (VEHICLE CONTROL)
ANIMAL NUMBER
BODY W.
BRAIN
HEART
105 217 106 272 107 227
1.9 8 2 .0 5 1.8 5
0 .8 6 7 0 .960 0.868
LIVER
6 .95 9 .23 6 .9 0
O W -IN D - 13 3 O -M A R -9 9
KIDNEYS
ADRENALS SPLEEN
OVARIES
1.5 1 1 .7 1 1.3 9
0 .0 52 0.0 56 0 .0 57
0 .449 0 .439 0 .372
0 .150 0 .574 0 .132
GROUP 2 (TELOMER ALCOHOL)
ANIMAL NUMBER
BODY W.
BRAIN
HEART
1 2 1 200 122 203 123 231
1.8 5 1.8 5 1.9 8
0 .8 12 0 .9 15 0 .8 4 8
LIVER
6 .72 6.01 7.7 2
KIDNEYS
ADRENALS SPLEEN
OVARIES
1.6 0 1 .3 1 1.6 2
0.0 74 0.0 57 0 .0 57
0 .3 67 0 .3 7 0 0 .536
0 .12 5 0 .13 5 0 .13 1
GROUP 3 (TELOMER ACRYLATE)
ANIMAL NUMBER
BODY W.
BRAIN
HEART
LIVER
137 217 138 189 1 3 9 200
1.88 1.9 1 1.9 4
0 .873 0 .76 7 0 .853
7.4 0 5 .6 6 6 .0 6
KIDNEYS
ADRENALS SPLEEN
OVARIES
1.7 0 1.4 1 1.4 4
0 .0 8 0 0.0 47 0.0 67
0 .5 2 3 0 .382 0 .3 4 4
0.120 0.120 0 .138
GROUP 4 (
)
ANIMAL NUMBER
BODY W.
BRAIN
153 2 14 '
154 246 155 191
1.8 9 1.9 5 1.9 0
HEART
0 .9 8 0 0 .9 5 0 0 .878
LIVER
KIDNEYS
ADRENALS SPLEEN
OVARIES
7.0 5 7.0 0
7 .18
' 1.6 1 1.7 2
1.4 8
0 .0 75 0 .0 6 5
0 .0 6 8
0 .4 0 4 0 .4 3 2
0 .4 0 5
0 .14 9 0 .16 2
0 .12 3
GROUP 5 (PFHS)
ANIMAL NUMBER
BODY W.
BRAIN
169 228 170 211 1 7 1 232
1.9 0 1.9 2 L . 95
HEART
0 .844 0 .78 4 0 .8 9 2
LIVER
6 .77 6.8 4 7.9 6
KIDNEYS
ADRENALS SPLEEN
OVARIES
1.5 4 1.5 5 1.4 5
0 .0 61 0 .0 55
0 .059
0 .3 6 2 0 .5 0 8 0 .476
0 .13 5 0 .118 0 .14 7
294
BACK TO MAIN
NOTOX Project 242933
___
ORGAN WEIGHTS (GRAM) AFTER 2 WEEKS RECOVERY FEMALES
GROUP 6 (PFOS)
ANIMAL NUMBER
BODY W.
BRAIN
HEART
185 197 186 199 18 7 203
2.02 1.9 3 2.0 4
0.70 2 0 .73 5 0 .732
LIVER
8 .18 7 .10 7.3 7
OW-IND - 14 3O-MAR-9 9
*
KIDNEYS
ADRENALS SPLEEN
OVARIES
1.4 8 1.4 1 1.2 6
0 .0 57 0.0 56 0 .0 5 5
0 .397 0 .4 15 0 .365
0 .138 0 .132 0 .12 7
295
BACK TO MAIN
NOTOX Project 242933
_
ORGAN/BODY WEIGHT RATIOS (%) AFTER 2 WEEKS RECOVERY FEMALES
GROUP 1 (VEHICLE CONTROL)
ANIMAL NUMBER
BODY W.
BRAIN
(GRAM) '
HEART
LIVER
105 217 106 272 107 227
0 .9 1 0 .75 0 .8 1
0 .4 0 0 0 .353 0 .3 8 2
3 .2 0
3 .39 3 .0 4
O W - I N D - 15 30-MAR-99
-
KIDNEYS
ADRENALS SPLEEN
OVARIES
0 .69 0 .6 3 0 .6 1
0 .0 2 4 0.021 0 .0 2 5
0.20 7 0 .16 1 0 .164
0 .0 69 0.211 0.0 58
GROUP 2 (TELOMER ALCOHOL)
ANIMAL NUMBER
BODY W. (GRAM)
BRAIN
HEART
1 2 1 200 122 203 123 231
0 .9 3 0 .9 1 0.86
0 .406
0 .4 5 1 0 .367
LIVER
KIDNEYS
ADRENALS SPLEEN
OVARIES
3 .3 6
0 .8 0
2 .9 6
0 .65
3 .3 4 0 .70
0 .0 37 0 .0 2 8 0 .0 2 5
0 .18 4 0 .182 0 .2 3 2
0.0 63 0 .0 67 0 .0 57
GROUP 3 (TELOMER ACRYLATE)
ANIMAL NUMBER
BODY W. (GRAM)
BRAIN
HEART
LIVER
137 217 138 189 1 3 9 200
0 .87 1.01 0 .97
0 .4 0 2 0 .4 0 6 0 .42 7
3 .4 1 3.0 0 3.0 3
KIDNEYS
ADRENALS SPLEEN
OVARIES
0 .79 0 .75 0 .72
0 .0 3 7 0 .0 2 5 0 .0 3 4
0 .2 4 1 0.202 0 .172
0 .0 55 0 .0 63 0 .0 69
GROUP 4 ( )
ANIMAL NUMBER
BODY W. (GRAM)
BRAIN
153 214' 154 246 155 191
0.88 0 .79
0 .99
HEART
0 .458 0 .3 8 6 0 .4 6 0
LIVER
3 .2 9 2 .8 5 3 .76
KIDNEYS
ADRENALS SPLEEN
OVARIES
0 .75 0 .70
0 .77
0 .0 3 5 0 .0 2 6
0 .0 3 6
0 .18 9 0 .176
0.212
0.0 70 0 .0 66
0 .064
GROUP 5 (PFHS)
ANIMAL NUMBER
BODY W. (GRAM)
BRAIN
169 228 170 211 1 7 1 232
0 .8 3 0 .9 1 0 .8 4
HEART
0 .3 7 0 0 .372 0 .384
LIVER
2 .9 7 3 .2 4 3 .4 3
KIDNEYS
ADRENALS SPLEEN
OVARIES
0.68 0 .74 0 .62
0 .0 2 7 0 .0 2 6 0 .0 2 5
0 .159 0 .24 1 0.20 5
0 .0 59 0 .0 56 0 .0 63
296
BACK TO MAIN
NOTOX Project 242933
_^
ORGAN/BODY WEIGHT RATIOS (%} AFTER 2 WEEKS RECOVERY FEMALES
GROUP 6 (PFOS)
ANIMAL NUMBER
BODY W. (GRAM)
BRAIN
HEART
LIVER
185 197 186 199 18 7 203
1.02 0 .97 1.00
0 .3 56 0 .369 0 .3 6 1
4 .15 3 .5 7 3 .6 3
O W - I N D - 16 30-MAR-99
*
KIDNEYS
ADRENALS SPLEEN
OVARIES
0 .75 0 .71 0 .62
0 .0 2 9 0 .0 2 8 0.0 27
0.202 0.20 9 0 .18 0
0.0 70 0 .0 6 6 0 .0 63
257
BACK TO MAIN
NOTOX P r o j e c t 24 2 933
ORGAN WEIGHTS (GRAM) AFTER 4 WEEKS RECOVERY MALES
GROUP 1 (VEHICLE CONTROL)
ANIMAL NUMBER
BODY W.
BRAIN
HEART
13 415 14 360 15 392
1.9 7 1.8 3 2.12
1.2 8 4 1.2 0 6 1 .0 9 9 -
LIVER
1 2 .2 6 9 .5 7
10 .4 7
OW-IND - 17 3 0 - M A R - 99
KIDNEYS
ADRENALS SPLEEN
TESTES
2 .6 9 2 .38 2 .24
0 .0 3 8
0 .0 41 0 .058
0 .576
0 .6 11 0 .656
2.86
2 .73 3 .0 1
GROUP 2 (TELOMER ALCOHOL)
ANIMAL NUMBER
BODY W.
BRAIN
HEART
29 330 30 396 3 1 362 32
2.01 2 .0 8 2 .0 8
1.19 5 1.19 9 1.2 2 8
LIVER
9 .4 1 10 .2 7
9 .6 6
KIDNEYS
ADRENALS SPLEEN
TESTES
2 .3 5 2 .4 8 2 .4 4
0 .0 4 8 0 .0 59 0 .0 4 2
0 .456 0 .544 0 .6 2 0 ** "*
2 .9 7 2 .92 3 .6 8
----
GROUP 3 (TELOMER ACRYLATE)
ANIMAL NUMBER
BODY W.
BRAIN
HEART
LIVER
45 380 46 380 47 375
2.0 7 2.0 7 2.12
1.2 8 2 1.2 3 7 1.13 2
11.0 8 9 .77
10 .0 5
KIDNEYS
ADRENALS SPLEEN
TESTES
2 .5 5 2 .4 8 2 .50
0 .0 5 0 0 .0 5 6 0.0 57
0 .656 0 .534 0 .582
3 .0 6 3 .11 3 .17
GROUP 4 ( )
ANIMAL NUMBER
BODY W.
BRAIN
61 434 62 406 63 363
2 .3 3 1.9 9 2.00
HEART
1.3 78 1.3 8 9 1.12 9
LIVER
9 .9 1 10 .4 6
9 .8 1
KIDNEYS
ADRENALS SPLEEN
TESTES
2 .79
2 .32 2 .6 7
0 .0 7 0 0 .0 4 6 0 .0 51
0 .692 0 .70 5 0 .76 2
3 .3 6 2 .9 7 2 .9 8
GROUP 5 (PFHS)
ANIMAL NUMBER
BODY W.
BRAIN
77 358 78 321 79 326
2.11 1.9 5 1.9 9
HEART
1.10 5 0 .8 75 1.16 4
LIVER
9 .5 7 8 .43 8 .54
KIDNEYS
ADRENALS SPLEEN
TESTES
2 .13 1.8 9 2 .6 9
0 .0 5 0 0 .0 45 0.0 47
0 .625 0 .529 0 .5 0 2
3 .2 2 3.0 3 3 .0 1
298
BACK TO MAIN
NOTOX Project 242933
ORGAN WEIGHTS (GRAM) AFTER 4 WEEKS RECOVERY MALES
GROUP 6 (PFOS)
ANIMAL BODY W. BRAIN NUMBER
HEART
93 301 94 374 95 356
1.96 2.08 1.98
0.953 1.429 1.311
ZfjTVER
8.98 12.66 11.87
OW-IND - 18 30-MAR-9 9
KIDNEYS ADRENALS SPLEEN TESTES
2.26 2.66 2.53
0.055 0.037 0.047
0.505 0.774 0.607
3.10 3.62 3.09
299
BACK TO MAIN
NOTOX Project 242933
ORGAN/BODY WEIGHT RATIOS (%) AFTER 4 WEEKS RECOVERY HALES
GROUP 1 (VEHICLE CONTROL)
ANIMAL NUMBER
BODY W. (GRAM)
BRAIN
HEART
LIVER
13 415 14 360 15 392
0 .47
0 .5 1 0 .54
0 .309 0 .335 0 .2 8 0
2 .9 6 2.66 2 .6 7
GROUP 2 (TELOMER ALCOHOL)
ANIMAL NUMBER
BODY W. (GRAM)
BRAIN
HEART
29 330 30 396 31 362 32
0 .6 1 0 .52 0 .57
0 .3 6 2 0 .3 0 3 0 .339
LIVER
2 .8 5 2 .59 2 .6 7
OW -IN D - 1 9 3 0 -M A R -99
KIDNEYS
ADRENALS SPLEEN
TESTES
0 .65 0.66 0 .57
0 .0 0 9 0.011 0 .0 15
0 .139 0 .170 0 .16 7
0 .69 0 .76 0 .77
KIDNEYS
ADRENALS SPLEEN
TESTES
0 .71 0 .6 3 0 .67
0 .0 15 0 .0 15 0.012
0 .138 0 .13 7 0 .17 1
0 .90 0 .74 1.02
GROUP 3 (TELOMER ACRYLATE)
ANIMAL NUMBER
BODY W. (GRAM)
BRAIN
HEART
LIVER
45 380 46 380 47 375
0 .54 0 .55 0 .57
0 .337 0 .326 0 .3 0 2
2 .92 2 .5 7 2.68
'
KIDNEYS
ADRENALS SPLEEN
0 .67 0 .65 0 .67
0 .0 13 0 .0 15 0 .0 15
0 .173 0 .14 1 0 .155
TESTES
0 .8 0 0 .82 0 .8 5
GROUP 4 ( )
ANIMAL NUMBER
BODY W. (GRAM)
BRAIN
61 434 62 406 63 363
0 .54 0 .49 0 .55
HEART
0 .318 0 .3 4 2 0 .3 11
LIVER
2 .28 2 .58 2 .70
KIDNEYS
ADRENALS SPLEEN
TESTES
0 .64
0 .57 0 .74
0 .0 16 . 0.011
0 .0 14
0 .159 0 .174 0.210
0 .77
0 .73 0 .82
GROUP 5 (PFHS)
ANIMAL NUMBER
BODY W. (GRAM)
BRAIN
77 358 78 321 79 326
0 .59 0 .6 1 0 .6 1
HEART
0 .309 0 .273 0 .3 57
LIVER
2 .6 7 2 .6 2 2 .6 2
KIDNEYS
ADRENALS SPLEEN
TESTES
0 .5 9 0 .59 0 .8 3
'
0 .0 14 0 .0 14 0 .0 14
0 .175 0 .16 5 0 .154
0 .90 0 .94 0 .92
300
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NOTOX Project 242933
_
ORGAN/BODY WEIGHT RATIOS {%) AFTER 4 WEEKS RECOVERY MALES
GROUP 6 (PFOS)
ANIMAL NUMBER
BODVT W. (GRAMI
BRAIN
HEART
LIVER
93 301 94 374 95 356
0 .65 0 .56 0 .56
0 .3 17 0 .382 0 .3 6 8
2 .9 8 3 .39 3 .3 4
O M - I N D - 20 3 0 - M A R - 99
*
KIDNEYS
ADRENALS SPLEEN
TESTES
0 .75 0 .7 1 0 .7 1
0 .0 18 0.010 0 .0 13
0 .168 0.20 7 0 .17 1
1.0 3 0 .9 7 0 .8 7
301
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NOTOX Project 242933
ORGAN WEIGHTS (GRAM) AFTER 4 WEEKS RECOVERY FEMALES
GROUP 1 (VEHICLE CONTROL)
ANIMAL NUMBER
BODY W.
BRAIN
HEART
109 212 110 261 111 255
1.86 1.8 7 2.01
0 .8 4 7 1.0 52 0 .850
LIVER
5.8 3 8.12 7.0 9
O W -IN D - 2 1 3 0 -M A R -99
KIDNEYS
ADRENALS SPLEEN
OVARIES
1.3 4 1.8 4 1.6 0
0 .0 6 2
0 .426
0 .078
0 .508
0 .0 55 - 0.402
0 .13 7 0 .14 4 0 .118
GROUP 2 (TELOMER ALCOHOL)
ANIMAL NUMBER
BODY W.
BRAIN
HEART
125 223 1 2 6 220 127 241 123
2.00 1.8 9 2.00
0 .826 0 .75 7 0 .9 7 1
LIVER
7 .2 1 6.22 7.2 0
KIDNEYS
ADRENALS SPLEEN
OVARIES
1.6 2 1 .7 2 1.5 3
0 .0 7 9 0 .0 5 3 0 .0 61
0 .430 0 .3 0 9 0 .5 8 9
0.122 0 .13 8 0 .179
GROUP 3 (TELOMER ACRYLATE)
ANIMAL NUMBER
BODY W.
BRAIN
HEART
LIVER
1 4 1 212 142 211 143 254
1.8 3 1.86 1.9 4
0 .8 54 0 .834 0 .922
6 .6 9 6 .0 9 7 .7 9
KIDNEYS
ADRENALS SPLEEN
OVARIES
1.6 4 1.3 8 1.6 1
0 .0 6 8 . 0.053
0 .0 6 6
0 .429 0 .4 8 9 0 .360
0 .160 0 .138 0 .13 6
GROUP 4 ( )
ANIMAL NUMBER
BODY W.
BRAIN
157 219 158 211 159 275
2.02 1.9 4 1.9 4
HEART
0 .9 0 0 0 .836 1.0 18
LIVER
6 .0 4 6 .82 9 .0 4
KIDNEYS
ADRENALS SPLEEN
OVARIES
1.4 6
1.6 2 1.9 5
0 .0 61 0 .0 61 0 .0 6 2
0 .595
0 .3 9 1 0 .525
0 .12 5 0 .16 7 0 .20 4
GROUP 5 (PFHS)
ANIMAL NUMBER
BODY W.
BRAIN
173 219 174 241 175 219
1.8 5 1.9 7 1.8 1
HEART
0.866 0 .92 2 0 .793
LIVER
6 .99 6.88 6 .60
KIDNEYS
ADRENALS SPLEEN
OVARIES
1.7 5 1.5 8 1.5 2
'
0 .0 6 4 0 .0 6 8 0 .0 6 3
0 .3 77 0 .329 0 .358
0 .12 3 0 .128 0 .20 7
302
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NOTOX Project 242933
ORGAN WEIGHTS (GRAM) AFTER 4 WEEKS RECOVERY FEMALES
GROUP 6 (PFOS)
ANIMAL NUMBER
BODY W.
BRAIN
HEART
189 201 19 0 220 1 9 1 202
1.7 8 1.9 2 1.9 3
0 .854 0 .79 9 0 .8 2 3
LIVER
7.7 2 7 .6 1 7 .15
O W -IN D - 22 3 0 -M A R -99
KIDNEYS
ADRENALS SPLEEN
OVARIES
1.5 5 1.8 0 1.68
0 .0 6 9 0 .0 4 9 0 .0 5 5
0 .48 5 0 .4 3 1 0 .366
0 .13 9 0 .14 4 0 .14 1
303
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NOTOX Project 242933
ORGAN/BODY WEIGHT RATIOS (%) AFTER 4 WEEKS RECOVERY FEMALES
GROUP 1 (VEHICLE CONTROL)
ANIMAL NUMBER
BODY W. (GRAM)
BRAIN
HEART
LIVER
109 212 110 261 111 255
0.88 0 .7 1 0 .79
0 .4 0 0 0 .4 0 3 0 .333
2 .75 3 .11 2 .78
O W -IN D - 23 3 0 -M A R -9 9
KIDNEYS ' ADRENALS SPLEEN
OVARIES
0 .6 3 0 .70 0 .6 3
0 .0 2 9 0 .0 3 0 0.022
0.201 0 .19 5 0 .158
0 .0 65 0 .0 55 0 .0 46
GROUP 2 (TELOMER ALCOHOL)
ANIMAL NUMBER
BODY W. (GRAM)
BRAIN
HEART
125 223 1 2 6 220 127 241 128
0 .90 0.86 0 .8 3
0 .3 7 0 0 .3 4 4 0 .4 0 3
LIVER
3 .23 2 .8 3 2 .9 9
KIDNEYS
ADRENALS SPLEEN
OVARIES
0 .73 0 .78 0 .64
0 .0 3 5 0 .0 2 4 0.0 25
0 .19 3 0 .140 0 .244
0 .0 55 0 .0 63 0 .0 74
GROUP 3 (TELOMER ACRYLATE)
ANIMAL NUMBER
BODY W. (GRAM)
BRAIN
HEART
LIVER
1 4 1 212 142 211 143 254
0 .8 7 0.88 0 .76
0 .4 0 3 0 .395 0 .3 6 3
3 .15 2.8 9 3 .0 7
KIDNEYS
ADRENALS SPLEEN
OVARIES
0 .77 0.66 0 .6 3
0 .0 3 2 0.0 25 0 .026
0.202 0 .2 3 2 0 .142
0 .0 75 0.0 65 0 .0 54
GROUP 4 ( )
ANIMAL NUMBER
BODY W. (GRAM)
BRAIN
157 219 158 211
159 275
0 .92 0 .92 0 .70
GROUP 5 (PFHS)
ANIMAL NUMBER
BODY W. (GRAM)
BRAIN
173 174175
219 241 219
0 .8 5 0 .8 2 0 .83
HEART
0 .4 11 0 .396 0 .3 7 0
HEART
0 .3 9 5 0 .3 8 3 0 .3 6 2
LIVER
KIDNEYS
ADRENALS SPLEEN
OVARIES
2 .76 3 .2 3 3 .29
0 .6 7
0 .0 2 8
0 .77
0 .029
0 .7 1 0.023
'
0 .272 0 .18 5 0 .19 1
0 .0 57 0 .0 79 0 .0 74
LIVER
KIDNEYS
ADRENALS SPLEEN
OVARIES
3 .19 2.86
3 .0 2
0 .8 0 0 .65 0 .69
0 .029 0 .0 2 8 0 .029
0 .172 0 .13 7 0 .163
0 .0 56
0 .0 5 3 0.0 95
304
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NOTOX Project 242933
ORGAN/BODY WEIGHT RATIOS (%) AFTER 4 WEEKS RECOVERY FEMALES
GROUP 6 (PFOS)
ANIMAL BODY W. BRAIN NUMBER .(GRAM)
HEART
LIVER
189 201 190 220 191 202
0.88 0.87
0.96
0.425 0.363 0.407
3.84 3.46 3.54
O W - I N D - 24 3 0 - M A R - 99
*
KIDNEYS ADRENALS SPLEEN OVARIES
0.77
0.82 0.83
0.034 0.022 0.027
0.241
0.196 0.181
0.069 0.065 0.070
305
ProPath GmbH
APPENDIX 1
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NOTOX
Safety & Environmental Research B.V.
Pathology Report
Exploratory 28-Day Oral Toxicity Study with
Telomer Alcohol, Telomer Acrylate,
, PFHS and
PFOS (positive control)
by Daily Gavage in the Rat
followed by a 14/28-Day Recovery Period
NOTOX Project 242933
NOTOX Substances 83484, 83493, 83502, 84456 and 84141 3M Test no. T-7023, T-7024, T-7022, T-7032, T-6295
This report contains 146 pages
Dr. J.Th. Wilson Oberemattstr.52a Pratteln4133 Switzerland
tel: +41 61 823 0410 fax:+41 61 8230411
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PATHOLOGY REPORT
Page 2/146
Test Article: telomer alcohol/telomer acrylate/ /pfhs/pfos
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. :
242933
Propath no.:
' 99010
Date
: 21.Apr.1999
Table of Contents
Table of contents..................
2
Authentication..............................................
3
Summary.......................................................................... 4
Materials and methods.......................................................
4
R e s u l t s ...................
4
Conclusions .......................................................................4
Incidence table - macroscopicfindings .......................................... 4
Incidence table - selected microscopic findings with grades ................... 4
Incidence table - microscopicfindings .......................................... 4
Individual animal microscopic findings .......................................... 4
Animal data l i s t .................................................
4
Individual animaldata records ................................................... 4
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Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ VPFHS/PFOS
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. Propath no. Date
242933 99010
21.Apr.1999
Authentication
.
The undersigned hereby declares, that the histopathology data in this report were compiled by him, and that they reflect accurately the primary data records. This report, consisting of 146 pages, was created by the computer system of Propath GmbH.
Jeffrey Th. Wilson, Dr.med.vet, BVSc, MSc, MRCVS Toxicologic Pathologist
Propath GUbH CH-4133 Pratteln, Switzerland
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Test Article: telomer alcohol/telomer acrylate/ ./pfhs/pfos
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. Propath no. Date
242933 99010
21.A p r .1999
Summary
'.
-
P'athomorphologic examination was performed on.168 Sprague Dawley rats (84 males,
84 females) which had been subjected to an exploratory 28-day oral (gavage)
toxicity study with the test articles Telomer alcohol/ Telomer acrylate/
,/
PIUS/ PFOS.
The rats were assigned to' six dose groups each containing fourteen males and
fourteen females. Test articles were administered once daily by gavage at doses
of 30 (telomer alcohol, telomer acrylate and
), 10 (PFHS) and 3 mg/kg
(PFOS) (dose groups 2, 3, 4, 5 and 6 respectively) for 28 days. The rats of the
control group 1 received the vehicle, 1% aqueous carboxymethyl cellulose, alone.
At the end of the treatment period eight animals of each sex from all groups
were killed and subjected to complete necropsies (main study). Three males and
three females from each dose group were subjected to necropsy following 14-day or
28-day treatment-free periods (recovery allocation 1 and 2).
All rats were necropsied. Histopathologic examination was performed on adrenal
glands, kidneys, liver, lymph nodes - mandibular and mesenteric, ovaries,
pancreas, spleen, testes, thymus and all organs or tissues with macroscopic
findings.
.
All animals survived their allocated treatment periods.
Hepatocellular hypertrophy was present in group 2 (30 mg/kg/day telomer alcohol), group 5 (10 mg/kg/day PFHS) males and in both sexes of group 6 (3 mg/kg/day PFOS) at the end of the main study. In the pancreas a slight increase in the incidence of exocrine apoptosis/single cell necrosis was recorded in group 2 males. Following the recovery periods, the hepatocellular hypertrophy had undergone partial or complete resolution in group 2 and 5 males and in group 6 females. The incidence of pancreas apoptosis/single cell necrosis in group 2 males had also returned to the level of the vehicle control group.
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Test Article: telomer alcohol/telomer acrylate/, ../pfhs/pfos
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. Propath no. Date
242933 99010
21.Apr.1999
M a t e r ia ls a n d M eth od s
Study Design
D ose gro u p
i V eh icle con trol
D ose
mg/kg
N um ber o f r a t s
m ale s
. m ain
x e^c . l
fe m a le s
. m ain
r e^c . l
a 3/3
8 3/3
T elom er a lco h o l
30
8 3/3
8 3/3
Telom er a c ry la te
4
5 PFHS
6 PFOS
30
30 10 3
8 3/3
8 3/3 8 3/3 8 3/3
8 3/3
8 3/3 8 3/3 8 3/3
m ain 1 -8
A n im a l n u m b ers
m ale s r e c .l
ree. 2
Tnai n
fe m a le s r e c .l
rea. 2
9 -11
13 -15
97-104 10 5-10 7 1 0 9 -1 1 1
17-24
25-27
29-31
113-120 121-123 125-127
33-40 . 41-43
49-56 65-72 81-88
57-59 73-75 89-91
45-47
129-136 137-139 14 1-14 3
61-63 77-79 93-95
145-152 161-168 177-18 4
153-155 16 9 -17 1 18 5 -18 7
15 7 -15 9 1 7 3 --1 7 5 18 9 -19 1
Administration of the Test Article
The test article was administered daily by gavage for 28 days. The recovery group rats were allowed a 14-day (recovery 1) or 28-day (recovery 2) treatment free period. Rats of the control group 1 received the vehicle, IS aqueous carboxymethyl cellulose, alone.
Necropsy and Histopathology
At the end of the assigned study periods, the rats were killed by exsanguination following anesthesia by ether vapour. Complete necropsies were performed on all rats. Representative tissue samples of the following organs were preserved in 4% phosphate buffered neutral formaldehyde solution (10% formalin).
Adrenal glands, aorta, bone - sternum and femur including joint; bone marrow sternal, brain, clitoral glands, epididymides, esophagus, eyes with optic nerve and Harderian glands; heart, kidneys, lacrimal glands - exorbital, large intestine - cecum, colon and rectum; larynx, liver, lungs, lymph nodes mandibular and mesenteric; female mammary gland area, nasopharynx, ovaries, pancreas, pituitary gland, preputial glands, prostate gland, salivary glands mandibular and sublingual; sciatic nerve, seminal vesicles, skeletal muscle, skin, small intestine - duodenum, jejunum and ileum with Peyer's patches; spinal cord - cervical, midthoracic and lumbar; spleen, stomach, testes, thymus, thyroid glands with parathyroid glands, tongue, trachea, urinary bladder, uterus with uterine cervix, vagina and all organs or tissues with macroscopic abnormalities.
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Test Article: telomer alcohol/telomer acrylate/ /pfhs/peos
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. Propath no. Date
242933 99010
21.Apr.1999
Materials and Methods
.
Following fixation, the following organs from all animals, were trimmed, processed and embedded in paraffin wax. Sections were cut at a thickness of 2-4 micrometers and stained with hematoxylin and eosin. Numbers of sections made are given in parentheses.
Adrenal glands (2), kidneys (2), liver (2), lymph nodes - mandibular (2) and mesenteric (1), ovaries (2), pancreas (1), spleen (1), testes (2), thymus (1) and all organs or tissues with macroscopic findings.
Initially sections of the above organs from all main study rats were examined by light microscopy. Following this examination, sections of liver from groups 1 (vehicle control), 2 (telomer alcohol), 5 (PFHS) and 6 (PFOS) males and groups 1 and 6 females recovery allocations 1 and 2 were examined. Sections of pancreas from group 1 and 2 males of recovery allocations 1 and 2 were also examined.
The sections were in February/March, 1999.
Data Compilation
The animal data and macroscopic findings were electronically transferred from the necropsy raw data files of NOTOX into the computer system of Propath GmbH where the microscopic findings were recorded by the undersigned pathologist using on line input
Macroscopic findings are presented in summary in the table "Incidence of Macroscopic Findings" and in full descriptive terms in the "Individual Animal Data Records". Wherever possible, macroscopic findings were correlated with a microscopic finding.
Microscopic findings are listed for each animal along with severity grades in the table "Individual Microscopic Findings" and summarized in the tables "Incidence of All Microscopic Findings" and "Incidence Table - Selected Microscopic Findings with Grades". They are further given' in full descriptive terms in the "Individual Animal Data Records". Histologic changes were described according to their distribution, severity, and morphologic character.
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Test Article: telomer alcohol/telomer acrylate/ ,/pfhs/pfos NOTOX no.
Test System : Exploratory 28-Day Gavage Study in Rats Frbpath no.
Sponsor
: 3M Toxicology Services
DDaattee
242933 99010
21.Apr.1999
Results
Mortality
A female group 3 rat, no. 129, died at blood sampling on the day of scheduled
sacrifice. All other rats survived their assigned study periods.
.
Macroscopic Findings
The few macroscopic findings recorded at all sacrifices were unremarkable and did not distinguish treated rats from-the vehicle control group.
Microscopic Findings
Treatment related microscopic findings were noted in the liver and pancreas. At the end of the main study, midzonal/centrilobular hypertrophy was seen at a minimal or slight degree in six group 2 (telomer alcohol) males, minimal to moderate degrees in all group 5 (PFHS) males and all group 6 (PFOS) rats of both sexes. By the end of the 28-day recovery period (recovery 2)this finding had partially or completely resolved in group 2 and 5 males and in group 6 females, however remained in evidence in group 6 males.
In the pancreas a slight increase in the incidence of minimal or slight degrees of exocrine apoptosis/single cell necrosis was recorded in group 2 males at the end of the main study. The incidence of this finding returned to the level of the vehicle control group following the recovery periods.
The remainder of the microscopic findings recorded at both necropsies, were
within the range of background pathology encountered in Sprague Dawley rats of
this age and strain and occurred at similar incidences and severity in both
control and treated rats.
'
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Test Article: telomer alcohol/telomer acrylate/ /pfhs/pfos
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. Propath no. Date
242933 99010
21.Apr.1999
Conclusions
.
Morphologic alterations following the administration of Telomer alcohol/Telomer acrylate/ /PFHS/PFOS by once daily gavage to Sprague Dawley rats for twenty eight days, were present in the liver of telomer alcohol, PFHS and PFOS treated rats and in the pancreas of telomer alcohol treated rats.
Hepatocellular hypertrophy was present in group 2 (30 mg/kg/day telomer alcohol), group 5 (10 mg/kg/day PFHS) males and in both sexes of group 6 (3 mg/kg/day PFOS) at the end of the main study. In the pancreas a slight increase in the incidence of exocrine apoptosis/single cell necrosis was recorded in group 2 males. Following the recovery periods, the hepatocellular hypertrophy had undergone partial or complete resolution in group 2 and 5 males and in group 6 females. The incidence of pancreas apoptosis/single cell necrosis in group 2 males had also returned to the level of the vehicle control group.
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Test Article: telomer alcohol/telomer acrylate/' ,/pehs/pfos
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. :
242933
Pr&path n o .:
99010
Date
: 21.Apr.1999
Incidence Table -- Macroscopic Findings
M ain S tu d y SEX:
DOSE GROUP: num ber o f an im als Lungs H em orrhage Stom ach H em orrhage L ive r E n larg ed K id n evs P e lv ic d ila tio n T estes Reduced in s iz e E p id id ym id es Reduced in s iz e O v a rie s W atery c y s t U teru s C y st(s )
MALE
123 45 6
888888
00001 0
00001 0
00
001
0
000000
00001 0
00001 0
VEMAT.il! 123 4 5 6
888 8 8 8
000 0 0 0
000 0 0 0
000 0 0 0
01
00 0
0
1 000 0 0 000 1 0 0
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Test Article: telomer alcohol/telomer acrylate/ /pfhs/pfos
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. Prpath no. Date
242933 99010
21.Apr.1999
Incidence Table -- Macroscopic Findings .Recovery Allocation 1
SEX: DOSE GROUP:
num ber o f a n im als
L ive r
A ccen tu ated lo b u la r p attern
T estes
Reduced In s iz e
S k in
A lo p e c ia gen eral c h e s t re g io n
Scab fo rm a tio n ab d o m in al re g io n
M axs 1 2 3 45 6 3 3 3333
0 0 0 001
01
0 01
0
0 0 0 000 1 0 0000
0 0 0 000
JH2MU2 12345 6 33 3 3 3 3
00 000 0
01
000 0
000000
1 00000
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Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/
/PFHS/PFOS
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. Propath no. Date
242933 99010
21.Apr.1999
Incidence Table -- Macroscopic Findings JRecovexy Allocation 2
. SEX: DOSE GROUP:
num ber o f an im als
Eyes
Reduced in s iz e le n s
M A LS
1
23
4 S6
3 3 3 3 33
0 0 0 1 00
12 3 4 5 33 4 3 3
6 3
00000 0
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Test Article: telomer alcohol/telomer acrylate/ ,/pfhs/pfos
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. :
242933
Prtpath no.:
99010
Date
: 21.Apr.1999
Incidence Table - Selected Microscopic Findings with Grades
Main Study
SEX: DOSE GROUP:
num ber o f a n im als
L ive r
num ber exam ined
M id zo n al/ c e n trilo b u la r h yp ertrop h y, d iffu se (m in im al) (slig h t) (m od erate)
T o ta l
P an creas
num ber exam ined
A p o p to sis/ sin g le c e ll n e c r o s is , exo crin e (m in im al) (slig h t)
T o ta l
M US 12
3
45
6
8 8 8888
888888
123 45
6
88888 8
88888 8
05
00 0 0
0 1 002 2
0 000 66
0 60088
0000 0 1 000 0 0 4 0000 0 3
0000 0 8
88 8888
8888 8 8
1 4 2 02 1 1 201 01
2 62 1 2 2
20001
0
000 00 0
2 00 01
0
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Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ ,/PFHS/PFOS
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. Prbpath no. Date
242933 99010
21.A p r . 1999
Incidence Table - Selected Microscopic Findings with Grades
Recovery Allocation 2
SEX: DOSE GROUP:
num ber o f an im als
L ive r
MALS 1 2 '3
333
45 3. 3
6 3
12345 6 3 33 33 3
num ber exam ined
M id zo n al/ c e n trilo b u la r h ypertroph y, d iffu s e (m in im al) (slig h t) (m oderate)
T o ta l
33
01 00 00 01
33
3
10 11 02
23
0 0 0
0
3
1 0 0 1
Recovery Allocation 2
SEX: DOSE GROUP:
num ber o f a n im als
L ive r
num ber exam ined
M id zo n al/ c e n trilo b u la r h yp ertrop h y, d iffu s e (m in im al) (slig h t) (m od erate)
T o ta l
P a n creas
num ber exam ined
A p o p to sis/ sin g le c e ll n e c r o s is , exo crin e (m in im al)
MALS 1 23 45 6
33 3333
33
33
WVMAr.T? 12
3
45
6
3333 3 3
33
01 00 00 01
33
10
01 01 01
03
0 0 0
0
1 0 0
1
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Page 14/146
Test Article: telomer alcohol/elomR acrylate/, ./pfhs/pfos
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. Propath no. Date
242933 99010
21.Apr.1999
Incidence Table - Microscopic Findings
Main Study
SEX: DOSE GROUP:
number o f a n im als
Lungs
num ber exam ined
I n tr a - a lv e o la r b lo o d , necropsy a rte fa c t
V a scu la r m ine r a l i s a t io n , fo ca l
Stom ach
'
num ber exam ined
L ive r
num ber exam ined
In flam m ato ry c e l l fo c i
S in g le c e ll n e cro sis
M id zo n al/ c e n trilo b u la r h ypertroph y, d iffu s e
H e p a to ce llu la r v a cu o la tio n
H em ato p o ietic c e l l fo ci
C o a g u la tiv e n e c ro s is , fo ca l
P an creas
num ber exam ined
A p o p to sis/ sin g le c e ll n e c r o s is , exo crin e
E x o crin e atro p h y, fo ca l
to u s 123 45 6 888888
1 1 1
1
888888 888888 6663 66 0 60088
10003 4 3 00001 000010
8 88888 2 62 122 0 1 1 00 0
wn.'jwffl T.TS
12 3 4 5 6 888 8 8 8
8 8 8 .8 8 8 88888 8 7 65 3 5 4 000 0 0 8 10101 0 00010 0 00000 0
88888 8 2000 1 0 00100 0
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Test Article: telomer alcohol/telomer acrylate/ /pfhs/pfos
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. :
242933
Prbpath no.:
99010
Date
: 21.Apr.1999
Incidence Table - Microscopic Findings
SEX: DOSE GROUP:
num ber o f an im als
MALE 12
3
45
6
8 8 '8 8 8 8
P an creas
In fla m m a tio n , m ixed g ra n u lo c y tic / lym p h o cytic
000010
K id n eys
num ber exam ined
T u b u lar a tro p h y, c o rtic a l
In te rs titia l in fla m m atio n , lym p h o cytic
H yalin e c a s t(s )
T u b u lar m in e ra liza tio n
8 8888 8 3 55 34 6 3 12 13 2
1 10 20 2 0 1 00 0 0
P e lv ic in fla m m atio n , lym p h oid
0 0 1 00 0
P e lv ic d ila tio n
C y s tic tu b u le s
P e lv ic m in e ra liza tio n
T u b u lar e p it h e lia l h yp ertrop h y, fo c a l
000000 000011 010000 000000
T estes
num ber exam ined
S p e rm a tid ic g ia n t c e ll(s )
888888 1 000 01
S em in ifero u s tu b u la r atrop h y
100010
ZEM3XJ5
12 3 4 5 6 88888 8
0 000 0 0
8 888 8 8 4 12105 002103
0000 0 0 10000 0 000 0 0 0 01000 0 00011 0 1000 00 0000 1 0
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Test Article: telomer alcohol/ telomer acrylate/ . / pfh s/ pfos
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. Prpath no. Date
242933 99010
21.Apr.1999
Incidence Table - Microscopic Findings
SEX: DOSE GROUP:
MALS 12
3
num ber o f an im als
8 8 '8
E p id id ym id es
num ber exam ined
A trop h y
A sp erm ia '
O va ries
num ber exam ined
U teru s
num ber exam ined
C yst(s)
A d re n a l G lan d s
num ber exam ined
888
E x tr a c a p s u la r n od ule
000
V a cu o la tio n , m u ltifo c a l in z. fa s ic u la ta
000
A trop h y, c o r tic a l d iffu se
000
S p leen
.
num ber exam ined
888
H em o p o iesis, p rim a rily e ry th ro p o ie sis
888
H em osid erin pigm en t
67 5
Thvm us
num ber exam ined
888
Lym phoid a tr o p h y in v o lu tio n
.0 0 1
4S 88
1 1 1
88 11 00 00
88 88 74 8. 8 10
6 8
8 0 2 1 8 8 4 8 2
Eh42H*B 12
3
45
6
8888 8 8
88888 8
r l
88888 8 01 1 01 1 00000 0
000 00 0
88888 8 7 88 5 8 5
88888 8
888 113
88 11
8 1
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Test Article: telomer alcohol/ telomer acrylate/
/ pfhs/ pfos
Test System : Exploratory 28-Day Gavage Study in: Rats
Sponsor
: 3M Toxicology Services
NOTOX no. :
242933
Propath no.:
99010
Date
: 21.Apr.1999
In c id e n c e T a b le -- M ic r o s c o p ic F in d in g s
SEX: DOSE GROUP:
M&LB 1 23 45 6
nunfeer o f a n im a ls
B 8 '8 8 8 8
Thym us
L ym p h o cyto lysis, in cre a se d
001 1 00
M e s e n t e r ic Lymph Node
num ber exam ined
8888 88
C o n gestio n / e ry th ro p h a g o cy to sis
01
01
00
M a n d ib u la r Lvraoh Node
num ber exam ined
8888 88
P la sm a cy to sis
5 63242
Lym phoid h y p e r p la s ia
2001 00
C o n g estio n / e ry th ro p h a g o cy to sis
001 1 2 0
FEMALE i 2345 6 8888 8 8
01 1 1 1 0
888 8 8 8 00 0 0 0 0
8888 8 8 03 2 1 1 2 11 23 1 0 0000 0 0
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Test Article: telomer alcohol/T elomer acrylate/
. / pfhs/ pfos
NOTOX no. :
242933
Test System : Exploratory 28-Day Gavage Study in Rats ' Propath no.:
99010
Sponsor
: 3M Toxicology Services
Date
: 21.Apr.1999
Incidence Table - Microscopic Findings
Recovery Allocation 1
SEX: DOSE GROUP:
num ber o f a n im als
L ive r
num ber exam ined
In flam m ato ry c e l l fo c i
S in g le c e ll n e c ro sis
M id zo n al/ c e n trilo b u la r h yp ertrop h y, d iffu s e
H e p a to ce llu la r v a cu o la tio n
H em ato p o ietic c e l l fo c i
MALS 1 2 '3 4 5 6
- 3 3 3' 3 3 3
33 33
23 01
33 33
32 23
10 01
03 00
P a n creas
num ber exam ined
E x o crin e atro p h y, fo ca l
T estes
num ber exam ined Sem iniferous tu b u lar
atrop h y
S k in
num ber exam ined
In flam m atio n , e x u d a tiv e
33 01
1 1
1 1
T.TC
1 2345
6
3333 3 3
33 33
21 01
10 00
11 10
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Test Article: t e l o m e r a l c o h o l / t e l o m e r a c r y l a t e /', , / p f h s / p f o s
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. Prbpth no. Date
242933 99010
21.Apr.1999
Incidence Table - Microscopie Findings
Recovery Allocation 2
SEX: DOSE GROUP:
num ber o f a n im als
L ive r
num ber exam ined
In flam m ato ry c e l l fo ci
S in g le c e ll n e c ro sis
M id zo n al/ c e n trilo b u la r h yp ertrop h y, d iffu s e
H e p a to ce llu la r v a cu o la tio n
C o a g u la tiv e n e c r o s is , fo ca l
.
P a n creas
mis
1 23456 3 3 33 33
33 33
22 01
33 33
22 03
10 01
11 00
num ber exam ined
A p o p to sis/ sin g le c e ll n e c r o s is , ex o crin e
E yes
num ber exam ined
P e rio rb ita l in fla m m atio n due to b lo o d sam p lin g
33 10
1 1
FEMAU2
12345 6 33 3 3 3 3
33 33
12 01
00
0. 0
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Test Article: telomer alcohol/ telomer acrylate/
,/ pfhs/ pfos
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no.
242933
Prbpath no. '
99010
Date
21.Apr.1999
Individual Animal Microscopic Findings
Codes and symbols in table heading:
m Males f Females
Codes and symbols in animal lines:
p Planned sacrifice (main study) r Recovery 1 (plannedsacrifice) s Recovery 2 (plannedsacrifice) o Other
Grading system used in finding lines:
0 finding not present 1 minimal 2 slight 3 moderate 4 severe
5 very severe
x present Only organs/groups with findings are listed in the table
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Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PFOS
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. Ptpath no. Date
242933 ' 99010 21.A p r . 1999
Individual Animal Microscopia Findings
Hales, Dose group 1
12345 67 8 m .m m m m m m m
necropsy status Liver
PPPPP PPP
Inflammatory cell foci
22221122
Single cell necrosis Hepatocellular vacuolation
01111 12 0 00000 010
Hematopoietic cell foci
1 010 0 0 1 0
Pancreas
Apoptosis/single cell necrosis, 0 0 1 0 2 0 0 0 exocrine
Kidneys
Ttabular atrophy, cortical
0001010 1
Interstitial inflammation, lymphocytic
0000110 1
Hyaline cast(s)
000000 10
Testes
Spermatid!c giant cell(s)
0.1 0 0 0 0 0 0
Seminiferous tubular atrophy
0 1000000
Spleen
Hemopoiesis, primarily erythropoiesis
2 2211 12 1
Hemosiderin pigment
0 1112210
Mandibular Lymph Node
Plasmacytosis
2 2 22 03 0 0
Lymphoid hyperplasia
02200000
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Page 22/146
Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ . ./PFHS/PFOS
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. Prpath no. Date
242933 99010
21.A p r . 1999
Individual Animal Microscopic Findings
Hales, Bose group 1
necropsy status Liver
9 10 11 13 14 IS m .m m m m m
r r rsss
Inflammatory cell foci Single cell necrosis Hepatocellular vacuolation Pancreas
2 32222 0 2 1110 0 0 1100
Apoptosis/single cell necrosis, 0 0 0 0 0 1 exocrine
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Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/
/PFHS/PFOS
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. Pr'opath no. Date
242933 99010
21.Apr.1999
Individual Animal Microscopie Finding's
Males, Dosa group 2
necropsy status Liver
17 18 19 20 21 22 23 24 m .m m m m m m m
P P P P P P P .P
Inflammatory cell foci
Single cell necrosis
Midzonal/centrilobular hypertrophy, diffuse
1 2 122 2 2 2 1 00 11 1 1 1 0 11 112 0 1
Pancreas
Apoptosis/single cell necrosis, 2 1 0 1 0 2 1 1 exocrine
Exocrine atrophy, focal Kidneys
0 00 00 0 0 1
Tubular atrophy, cortical
Interstitial inflammation, lymphocytic
2 1011100 0 00 01 0 0 0
Hyaline cast(s) Tubular mineralization Pelvic mineralization
S p le e n
0 0 0 01 0 0 0 0 100000 0 01000000
Hemopoiesis, primarily erythropoiesis
1 11112 12
Hemosiderin pigment Mesenteric Lymph Node
0 1 121111
Congestion/erythrophagocytosis 0 0 1 0 0 0 0 0
Mandibular Lymph Node
Plasmacytosis
2 32 02022
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Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ ,/PFHS/PFOS
Test System : Exploratory 28-Day Gavage Study in Rats
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: 3M Toxicology Services
Page 24/146
NOTOX no. Propath no. Date
242933 99010
21.Apr.1999
Individual Animal Microscopic Findings
Hales, Dose group 2 necropsy status
25 26 27 29 30 31 m .m m m m m
r rr s s s
liver
Inflammatory cell foci
2 332 2 2
Single cell necrosis
1 110 1 1
Midzonal/centrilobular hypertrophy, diffuse
0 101 0 0
Hematopoietic cell foci
1 000 0 0
Coagulative necrosis, focal
0 000 1 0
Pancreas
Exocrine atrophy, focal Testes
0 0100 0
Seminiferous tubular atrophy
4
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Test Article: telomer alcohol/ telomer acrylate/
./ pfhs/ pfos
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. Propath no. Date
242933 99010
21.Apr.1999
Individual Animal Microscopic Findings
Males, Dose group 3
necropsy status Liver
33 34 35 36 37 38 39 40 m .m m m m m a m
P PPPPP PP
Inflammatory cell foci Single cell necrosis Pancreas
2 1123222 1 10 11 1 1 0
Apoptosis/single cell necrosis, 1 0 0 0 0 0 1 0 exocrine
Exocrine atrophy, focal Kidneys
0 000001 0
Tubular atrophy, cortical
Interstitial inflammation, lymphocytic
1. 0 0 1 1 0 1 1 0 101000 0
Pelvic inflammation, lymphoid
0 00000 1 0
Spleen
Hemopoiesis, primarily erythropoiesis
2 11122 2 1
Hemosiderin pigment Thymus
1 0001111
Lymphoid atrophy - involution Lymphocytolysis, increased Mandibular Lymph Node
0 0000100 0 0000002
Plasmacytosis
0 2 0 02 2 0 0
Congestion/erythrophagocytosis 0 0 0 0 0 0 0 3
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Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ ,/PFHS/PFOS
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. Propath no. Date
242933 99010
2 1 .Apr.1999
Individual Animal Microscopic Findings
Males, Bose group 4
necropsy status Liver
49 so 51 52 53 54 55 56 m . m m m m m mi a
PPPPPPPP
Inflammatory cell foci
2 111222 1
Single cell necrosis
1 10 0 0 X 0 0
Pancreas
Apoptosis/single cell necrosis, 0 0 0 0 0 0 2 0 exocrine
Kidneys
Tubular atrophy, cortical
Interstitial inflammation, lymphocytic
1000X001 0000X000
Hyaline cast(s)
00000101
Adrenal Glands
Extracapsular nodule Spleen
0 000000X
Hemopoiesis, primarily erythropoiesis
Hemosiderin pigment
Thymus
X 12 12 X2 X 011XXXXX
Lymphoid atrophy - involution Lymphocytolysis, increased Mesenteric Lymph Node
0000000X 0 0 0 0 0 0 0. 2
Congestion/erythrophagocytosis Mandibular Lymph Node
00010
000
Plasmacytosis
0 0 02 0 02 0
Lymphoid hyperplasia
0 0 02 0 00 0
Congestion/erythrophagocytosis 0 0 0 2 0 0 0 0
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Test Article: telomer alcohol/ telcmer acrylate/ . / pfh s/ pfo s
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. Propath no. Date
242933
99010 21 Apr.1999
Individual Animal Microscopic Findings
Males, Dose group 4 necropsy status
5 7 58 59 6 1 62 63
m .m m m m m
r rr s s s
Eyes
Periorbital inflammation due to blood sampling
3
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Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/' /PFHS/PFOS
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. Propath no. Date
242933 99010
21.A p r . 1999
Individual Animal Microscopic Findings
Males, Dosa group 5
necropsy status Lungs
Intra-alveolar blood, necropsy artefact
65 66 67 68 69 70 71 Ta m .m m m m m m m P P P PPP P P
X
Vascular mineralisation, focal
1
Liver Inflammatory cell foci Single cell necrosis Midzonal/centrilobular hypertrophy, diffuse Hepatocellular vacuolation Coagulative necrosis, focal
Pancreas
2 1 222222 10101 111 3 33332 2 3
2 0 1000 0 1 0 0 1000 0 0
Apoptosis/single cell necrosis, 0 1 0 1 0 0 0 0 exocrine
Inflammation, mixed granulocytic/lymphocytic
0 0 00010 0
Kidneys .
Tubular atrophy, cortical
Interstitial inflammation, lymphocytic
1 1 11 0 0 0 0 0 0 0 1110 0
Cystic tubules Testes
Seminiferous tubular atrophy Epididymides
0 0 00002 0
0 4000000 '
Atrophy Aspermia
3 X
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Page 29/146
Test Article: telomer alcohol/ telomr acrylate/
/ pfhs/ pfos
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. :
242933
Pfopath no.:
99010
Date
: 21.Apr.1999
Individual Animal Microscopio Findings
Males, Dose group 5
necropsy status Adrenal Glands
65 66 67 68 69 70 71 72 m .m m m m m m m
PPPPPPPP
Extracapsular nodule
0000X 000
Spleen
Hemopoiesis, primarily erythropoiesis
Hemosiderin pigment '
2 12 1 i2 1 2 0 102 0 0 12
Mandibular Lymph Node
Plasmacytosis
03200022
Congestion/erythrophagocytosis 0 1 0 0 0 0 0 2
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Page 30/146
Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PFOS
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. Propath no. Date
242933 99010
2 1 .Apr.1999
Individual Animal Microscopia Findings
Hales, Vose gxoup 5
necropsy status liver
73 74 75 77 78 79 m .m m m m m
rrrsss
Inflammatory cell foci
Single cell necrosis
Midzonal/centrilobular hypertrophy, diffuse
3 22222 2 11110 201000
Hepatocellular vacuolation Testes
0 0000 1
Seminiferous tubular atrophy
3
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Test Article: t e l o m e r a l c o h o l / t e l c m e r a c r y l a t e /
/ pfhs/ pfos
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. :
242933
Propath no.:
99010
Date
: 21 Apr.1999
Individual Animal Microscopic Findings
Hales, Lose group 6
necropsy status Liver
81 82 83 84 85 86 87 88 m mmmmmm m
p P P PPP P P
In fla m m a to ry c e l l f o c i
Single cell necrosis
Midzonal/centrilobular hypertrophy, diffuse
2 2 22 12 2 2 1 0 1101 1 1 2333323 3
Hepatocellular vacuolation Hematopoietic cell foci Pancreas
0110012 0 1 0 0000 0 0
Apoptosis/single cell necrosis, 0 2 0 0 0 0 0 1 . exocrine
Kidneys
Tubular atrophy, cortical
1101111 0
Interstitial inflammation, lymphocytic
0 021000 0
Hyaline cast(s) Cystic tubules
T estes
0 0 0001 1 0 0 020000 0
Spermatidic giant cell(s) Adrenal Glands
0 010000 0
Vacuolation, multifocal in z. fasiculata
0 0 00010 1
Atrophy, cortical diffuse
0 0 02000 0
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Page 32/146
Test Article: t e l o m e r a l c o h o l / t e l o m e r a c r y l a t e /
/ pfhs/ pfos
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. Prbpath no. Date
242933 99010
2 1 .Apr.1999
Individual Animal Microscopic Findings
MAZes, Dose group 6
necropsy status Spleen
.
81 82 83 84 85 86 87 m .m m m m m m
PPPPPPP
Hemopoiesis, primarily erythropoiesis
1 1 1 112 1
Hemosiderin pigment
1110000
Thymus
Lymphoid atrophy - involution
00000201
.Mandibular Lymph Node
.
Plasmacytosis
30020000
88 m P
1
1
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Page 33/146
Test Article: t e l o m e r a l c o h o l / e l o m r a c r y l a t e /
,'pfhs/ pfos
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. Propath no. Date
242933 99010
21.Apr.1999
I n d iv id u a l A n im a l M ic r o s c o p ic F in d in g s
Malas, Dosa group 6 necropsy status
89 90 91 93 94 95 m mmmm m
r r r `s 3 3
Liver
Inflammatory cell foci
Single cell necrosis
Midzonal/centrilobular hypertrophy, diffuse
2 22i2 2 1 10i0 1 2 33i32
Hepatocellular vacuolation
1 22010
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Page 34/146
Teat Article: t e l o m e r a l c o h o l / t e l o m e r a c r y l a t e /
/ pfhs/ pfos
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. :
242933
Propath no.:
99010
Date
: 21.Apr.1999
Individual Animal Microscopic Findings
Females, Dose group 1
necropsy status Liver
97 98 99 100 101 102 103 104 f .f f f f f f f
P P Pp p PP P
Inflammatory cell foci
2 121222 1
Single cell necrosis
1 0 111 11 1
Hepatocellular vacuolation
0 000000 1
Pancreas
Apoptosis/single ceil necrosis, 0 1 1 0 0 0 0 0 exocrine
Kidneys
Tubular atrophy, cortical Tubular mineralization Pelvic mineralization Spleen
10010101 0 0 00 100 0 0 100000 0
Hemopoiesis, primarily erythropoiesis
11110111
Hemosiderin pigment Thymus
Lymphoid atrophy - involution Mandibular Lymph Node
3 2223322 0 0000010
Lymphoid hyperplasia
0 0002000
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Page 35/146
Test Article: t e l o m e r a l c o h o l / t e l o m e r a c r y l a t e /
/pfhs/ pfos
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. Propath no. Date
242933 99010
21.Apr.1999
Individual Animal Microscopic Findings
Females, Dose group 1 necropsy status
105 106 107 109 110 111 f .f f f f f
r rrss s
Liver
Inflammatory cell foci
22222 1
Single cell necrosis Hepatocellular vacuolation
110100 020000
Skin Inflammation, exudative
2
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Test Article: t e l o m e r a l c o h o l / t e l o m e r a c r y l a t e /
,/pfhs/ pfos
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. Prbpath, no. Date
242933 99010
21.A p r . 1999
Individual Animal Microscopic Findings
Females/ Dosa group 2
necropsy status Liver
113 114 115 116 117 118 119 120 f . f f f f f f f
P PPPPP P P
Inflammatory cell foci Single cell necrosis
2 2111112 1 101011 1
Kidneys
Tubular atrophy, cortical
0 000001 0
Pelvic dilation
0 000X 0 0 0
Adrenal Glands
Extracapsular nodule
0 0 00X 0 0 0
Spleen
Hemopoiesis, primarily erythropoiesis
1111i11 1
Hemosiderin pigment
2222223 2
Thymus
Lymphoid atrophy - involution
0000010 0
Lymphocytolysis, increased
M a n d ib u la r Lymph N ode
0000001 0
Plasmacytosis
2 2 00030 0
Lymphoid hyperplasia
2 000000 0
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Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ ./PFHS/PFOS
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. Propath no. Date
242933 99010
21.A p r . 1999
Individual Animal Microscopic Findings
Females, Dose group 3
necropsy status Liver
129 130 131 132 133 134 135 136 f .f f f f f f f
oP P PPP P P
Inflammatory cell foci Single cell necrosis Hepatocellular vacuolation Pancreas Exocrine atrophy, focal Kidneys Tubular atrophy, cortical Interstitial inflammation,
lymphocytic Adrenal Glands
22 1121 12 1 1 102 0 0 1 0 1 000 0 0 0
0 0 0100 0 0
01000100 0000010 1
Extracapsular nodule Spleen
0 000000X
Hemopoiesis, primarily erythropoiesis
2 1 11211 i
Hemosiderin pigment Thymus
Lymphoid atrophy - involution Lymphocytolysis, increased Mandibular Lymph Node
1 2 2 12 22 3
0 100100 1 0 0 00010 0
Plasmacytosis Lymphoid hyperplasia
0 0000022 0 0000022
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Page 38/146
Test Article: telomer alcohol/ telomer acrylate/
/ pfhs/ pfos
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. Propth no. Date
242933 99010
21.Apr.1999
Individual Animal Microscopic Findings
Females, Dose group 4
necropsy status Liver
145 146 147 148 149 150 151 152 f . f f f f f f f
P PPPPPPP
Inflammatory cell foci Single cell necrosis Hematopoietic cell foci Kidneys
1 2 1 2 2 2 2 1 0 1100 0 0 1 0 0000 100
Tubular atrophy, cortical
Interstitial inflammation, lymphocytic
0 0000001 0 0010000
Cystic tubules Uterus
0 0200 0 0 0
Cyst(a) Spleen
X
Hemopoiesis, primarily erythropoiesis
1 10110 0 1
Hemosiderin pigment Thymus
2 22223 2 2
Lymphoid atrophy - involution Lymphocytolysis, increased Mandibular Lymph Node
0 0000001 0 0010000
Plasmacytosis Lymphoid hyperplasia
0 0000200 2 0200200
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Page 39/146
Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PFOS
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. Prpath no.' Date
242933 99010
21.Apr.1999
Individual Animal Microscopic Findings
Females, Dose group 5 '
necropsy status Liver
Inflammatory cell foci Single cell necrosis
161 162 163 164 165 166 167 168 f fffff f f p PPPP P P P
223211 1 1 011010 1 1
Hepatocellular vacuolation
000000 0 1
Pancreas
Apoptosis/single cell necrosis, 0 0 0 0 . 1 0 0 0 exocrine
Kidneys
Cystic tubules
0 00000 0 1
Tubular epithelial hypertrophy, focal
1
00000 0 0
Adrenal Glands
Extracapsular nodule Spleen
0 0000X 0 0
Hemopoiesis, primarily erythropoiesis
H em o sid erin p ig m en t
Thymus
112111 1 1 2 22222 2 3
Lymphoid atrophy - involution
0 00200 0 0
Lymphocytolysis, increased
0 0 1000 0 0
Mandibular Lymph Node
Plasmacytosis Lymphoid hyperplasia
0 020000 0 0000020 0
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Test Article: t e l o m e r a l c o h o l / t e l c m e r a c r y l a t e / / p f h s / p f o s
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. Propath no. Date
242933 ` 99010 21.A p r . 1999
Individual Animal Microscopic Findings
Females, Dose group
necropsy status Liver
177 178 179 180 181 182 183 184 f .f f f f f f f
P P P P P P 'P P
Inflammatory cell foci
Single cell necrosis
Midzonal/centrilobular hypertrophy, diffuse
2 1221111 10101010 22312 332
Kidneys
Tubular atrophy, cortical
1 1001 1 0 1
Interstitial inflammation, lymphocytic
01010010
Adrenal Glands
Extracapsular nodule
X 0000000
Spleen
Hemopoiesis, primarily erythropoiesis
i 0 011 1 0 1
Hemosiderin pigment
2 132322 2
Thymus
Lymphoid atrophy - involution
10000000
Mandibular Lymph Node
Plasmacytosis
0 0 00022 0
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Test Article: t e l o m e r a l c o h o l / t e l o m e r a c r y l a t e /
/ pfhs/ pfos
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. Propath no. Date
242933 99010'
21.Apr.1999
Individual Animal Microscopic Findings
females, Dose group 6 necropsy status
185 186 187 189 190 191 f .f f f f f
r rr3ss
Liver
Inflammatory cell foci
Single cell necrosis
Midzonal/centrilobular hypertrophy, diffuse
1 12211 0 0 1 1 1' 0 0 10001
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Test Article: t e l o m e r a l c o h o l / t e l o m e r a c r y l a t e /
/ pfhs/ pfos
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. :
242933
Propath no.:
99010
Date
: 21.Apr.1999
Animal Data List
Males
ANIMAL SEX FINAL TEST FIRST
LAST
NUMBER M/F STATE DATS DAT . DAT
DATE NECROPSY
DOSE GROUP 1
1 zn p 2m p 3m p 4m p . 5m p 6m p 7m p 8m p 9m r
10 m r
11 m r
13 m 3
14 za 3
15 a 3
DOSE GROUP 2
17 m P 18 m P 19 za P 20 za P 21 za P 22 za P 23 in P 24 m P 25 za r
26 za r
27 za r
29 m 3
30 za 3
31 za 3
DOSE GROUP 3 33 za P 34 za P 35 in P 36 za P 37 za P 38 zn P 39 za P 40 za P 41 za r
42 za r
43 zn r
45 za 3
46 zn 3
47 za 3
28 06/10/98 02/11/98 03/11/98 28 06/10/98 02/11/98 03/11/98 28 06/10/98 02/11/98 03/11/98 28 06/10/98 02/11/98 03/11/98 29 06/10/98 03/11/98 04/11/98 29 06/10/98 03/11/98 04/11/98 29 06/10/98 03/11/98 04/11/98 29 06/10/98 03/11/98 04/11/98 29 06/10/98 03/11/98 18/11/98 29 06/10/98 03/11/98 18/11/98 29 06/10/98 03/11/98 18/11/98 29 06/10/98 03/11/98 02/12/98 29 06/10/98 03/11/98 02/12/98 29 06/10/98 03/11/98 02/12/98
28 06/10/98 02/11/98 03/11/98 28 06/10/98 02/11/98 03/11/98 28 06/10/98 02/11/98 03/11/98 28 06/10/98 02/11/98 03/11/98 29 06/10/98 03/11/98 04/11/98 29 06/10/98 03/11/98 04/11/98 29 06/10/98 03/11/98 04/11/98 29 06/10/98 03/11/98 04/11/98 29 06/10/98 03/11/98 18/11/98 29 06/10/98 03/11/98 18/11/98 29 06/10/98 03/11/98 18/11/98 29 06/10/98 03/11/98 02/12/98 29 06/10/98 03/11/98 02/12/98 29 06/10/98 03/11/98 02/12/98
28 06/10/98 02/11/98 03/11/98 28 06/10/98 02/11/98 03/11/98 28 06/10/98 02/11/98 03/11/98 28 06/10/98 02/11/98 03/11/98 29 06/10/98 03/11/98 04/11/98 29 06/10/98 03/11/98 04/11/98 29 06/10/98 03/11/98 04/11/98 29 06/10/98 03/11/98 04/11/98 29 06/10/98 03/11/98 18/11/98 29 06/10/98 03/11/98 18/11/98 29 06/10/98 03/11/98 18/11/98 29 06/10/98 03/11/98 02/12/98 29 06/10/98 03/11/98 02/12/98 29 06/10/98 03/11/98 02/12/98
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Page 43/146
Test Article: telomer alcohol/ telomer acrylate/
./pfhs/ pfos
NOTOX no. :
242933
Test System : Exploratory 28-Day Gavage Study in Rats Propath no.:
99010
Sponsor
: 3M Toxicology Services
Date
: 21.Apr.1999
A n im a l D a ta L i s t
Males
:mal SEX FINAL TEST FIRST
LAST
EER M/F STATE DAYS DAY . DAY
DATE NECROPSY
DOSS GROUP 4
49 m P SO m P 51 m P 52 m P S3 m P 54 a P 55 m P 56 m P 57 m r
58 m r
59 m r
61 m s
62 m s
63 m s DOSS GROUP 5C
65 m P 66 za P 67 m P 68 m P 69 m P 70 zn P 71 xa P 72 m P 73 m r
74 m r
75 za r
77 za s
78 za s
79 za s DOSE GROUP CO6
81 za P 82 za P 83 za P 84 za P 85 za P 86 za P 87 za P 88 za P 89 za r
90 za r
91 za r
93 za s
94 za s
95 za s
28 06/10/98 02/11/98 03/11/98 28 06/10/98 02/11/98 03/11/98 28 06/10/98 02/11/98 03/11/98 28 06/10/98 02/11/98 03/11/98 29 06/10/98 03/11/98 04/11/98 29 06/10/98 03/11/98 04/11/98 29 06/10/98 03/11/98 04/11/98 29 06/10/98 03/11/98 04/11/98 29 06/10/98 03/11/98 18/11/98 29 06/10/98 03/11/98 18/11/98 29 06/10/98 03/11/98 18/11/98 29 06/10/98 03/11/98 02/12/98 29 06/10/98 03/11/98 02/12/98 29 06/10/98 03/11/98 02/12/98
28 06/10/98 02/11/98 03/11/98 28 06/10/98 02/11/98 03/11/98 28 06/10/98 02/11/98 03/11/98 28 06/10/98 02/11/98 03/11/98 29 06/10/98 03/11/98 04/11/98 29 06/10/98 03/11/98 04/11/98 29 06/10/98 03/11/98 04/11/98 29 06/10/98 03/11/98 04/11/98 29 06/10/98 03/11/98 18/11/98 29 06/10/98 03/11/98 18/11/98 29 06/10/98 03/11/98 18/11/98 29 06/10/98 03/11/98 02/12/98 29 06/10/98 03/11/98 02/12/98 29 06/10/98 03/11/98 02/12/98
28 06/10/98 02/11/98 03/11/98 28 06/10/98. 02/11/98 03/11/98 28 06/10/98 02/11/98 03/11/98 28 06/10/98 02/11/98 03/11/98 29 06/10/98 03/11/98 04/11/98 29 06/10/98 03/11/98 04/11/98 29 06/10/98 03/11/98 04/11/98 29 06/10/98 03/11/98 04/11/98 29 06/10/98 03/11/98 18/11/98 29 06/10/98 03/11/98 18/11/98 29 06/10/98 03/11/98 18/11/98 29 06/10/98 03/11/98 02/12/98 29 06/10/98 03/11/98 02/12/98 29 06/10/98 03/11/98 02/12/98
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Test Article: t e l o m e r a l c o h o l / t e l o m e r a c r y l a t e /
./p f h s / pf o s
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. :
Propath no.:
Date
:
242933 99010
21.Apr.1999
Animal Data List
F e m a le s
ANIMAL SEX FINAL TEST FIRST NUMBER M/F STATE DAYS DAY .
LAST DAY
DATE NECROPSY
DOSE GROUP 1 97 f 98 99 f
100 f 101 f 102 f 103 104 f 105 106 f 107 r 109 110 111 DOSE GROUP 2
113 114 115 116 117 118 119 120 121 122 123 125 126 127 DOSE GROUP 3 129 130 131 132 f 133 f 134 . 135 136 137 138 139 f 141 142 143
P P P P P P P P r E r s s s
P P P P P P P P r r r s s s
P P P P P P P r r r 3 s 3
28 08/10/98 04/11/98 05/11/98 28 08/10/98 04/11/98 05/11/98 28 08/10/98 04/11/98 05/11/98 28 08/10/98 04/11/98 05/11/98 29 08/10/98 05/11/98 06/11/98 29 08/10/98 05/11/98 06/11/98 29 08/10/98 05/11/98 06/11/98 29 08/10/98 05/11/98 06/11/98 29 08/10/98 05/11/98 20/11/98 29 08/10/98 05/11/98 20/11/98 29 08/10/98 05/11/98 20/11/98 29 08/10/98 05/11/98 04/12/98 29 08/10/98 05/11/98 04/12/98 29 08/10/98 05/11/98 04/12/98
28 08/10/98 04/11/98 05/11/98 28 08/10/98 04/11/98 05/11/98 28 08/10/98 04/11/98 05/11/98 28 08/10/98 04/11/98 05/11/98 29 08/10/98 05/11/98 06/11/98 29 08/10/98 05/11/98 06/11/98 29 08/10/98 05/11/98 06/11/98 29 08/10/98 05/11/98 06/11/98 29 08/10/98 05/11/98 20/11/98 29 08/10/98 05/11/98 20/11/98 29 08/10/98 05/11/98 20/11/98 29 08/10/98 05/11/98 04/12/98 29 08/10/98 05/11/98 04/12/98 29 08/10/98 05/11/98 04/12/98
28 08/10/98 04/11/98 05/11/98 28 08/10/98 04/11/98 05/11/98 28 08/10/98 04/11/98 05/11/98 28 08/10/98 04/11/98 05/11/98 29 08/10/98 05/11/98 06/11/98 29 08/10/98 05/11/98 06/11/98 29 08/10/98 05/11/98 06/11/98 29 08/10/98 05/11/98 06/11/98 29 08/10/98 05/11/98 20/11/98 29 08/10/98 05/11/98 20/11/98 29 08/10/98 05/11/98 20/11/98 29 08/10/98 05/11/98 04/12/98 29 08/10/98 05/11/98 04/12/98 29 08/10/98 05/11/98 04/12/98
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Page 45/146
Test Article: t e l o m e r a l c o h o l / t e l o m e r a c r y l a t e /
/ pfhs/ pfos
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. :
242933
Propath no.:
99010
Date
: 21.Apr.1999
Animal Data List
females ANIMAL SEX FINAL TEST FIRST NUMBER M/F STATE DAYS DAY
LAST DAY
DATE NECROPSY
DOSE GROUP 4
145 f P 146 P 147 f P 148 f P 149 f P 150 f P 151 f P 152 f P . 153 r
154 f r
155 f r
157 s
158 s
159 f s
DOSE GROUP 5
161 f P 162 P 163 f P 164 P 165 P 166 f P 167 f P 168 f P 169 f r
170 f r
171 r
173 3
' 174 s
175 s DOSE GROUP 6
177 P 178 f P 179 P 180 P 181 f P 182 P 183 P 184 f P 185 r
186 r
187 r
189 f 3
190 s
191 3
28 08/10/98 04/11/98 05/11/98 28 08/10/98 04/11/98 05/11/98 28 08/10/98 04/11/98 05/11/98 28 08/10/98 04/11/98 05/11/98 29 08/10/98 05/11/98 06/11/98 29 08/10/98 05/11/98 06/11/98 29 08/10/98 05/11/98 06/11/98 29 08/10/98 05/11/98 06/11/98 29 08/10/98 05/11/98 20/11/98 29 08/10/98 05/11/98 20/11/98 29 08/10/98 05/11/98 20/11/98 29 08/10/98 05/11/98 04/12/98 29 08/10/98 05/11/98 04/12/98 29 08/10/98 05/11/98 04/12/98
28 08/10/98 04/11/98 05/11/98 28 08/10/98 04/11/98 05/11/98 28 08/10/98 04/11/98 05/11/98 28 08/10/98 04/11/98 05/11/98 29 08/10/98 05/11/98 06/11/98 29 08/10/98 05/11/98 06/11/98 29 08/10/98 05/11/98 06/11/98 29 08/10/98 05/11/98 06/11/98 29 08/10/98 05/11/98 20/11/98 29 08/10/98 05/11/98 20/11/98 29 08/10/98 05/11/98 20/11/98 29 08/10/98 05/11/98 04/12/98 29 08/10/98 05/11/98 04/12/98 29 08/10/98 05/11/98 04/12/98
28 08/10/98 04/11/98 05/11/98 28 08/10/98 04/11/98 05/11/98 28 08/10/98 04/11/98 05/11/98 28 08/10/98 04/11/98 05/11/98 29 08/10/98 05/11/98 06/11/98 29 08/10/98 05/11/98 06/11/98 29 08/10/98 05/11/98 06/11/98 29 08/10/98 05/11/98 06/11/98 29 08/10/98 05/11/98 20/11/98 29 08/10/98 05/11/98 20/11/98 29 08/10/98 05/11/98 20/11/98 29 08/10/98 05/11/98. 04/12/98 29 08/10/98 05/11/98 04/12/98 29 08/10/98 05/11/98 04/12/98
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PATHOLOGY REPORT
Page 46/146
Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PFOS
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. Propath no. Date
242933 99010
21.Apr.1999
Individual Animal Data Records
Animal No: SEX: DOSE GROUP:
1 Male Vehicle Control
MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS Liver
Spleen Mandibular Lymph Node/
1 Mandibular Lymph Node/
2
Animal No:
2
M ACROSCOPIC FINDINGS
days of treatment :28
sacrifice group
:terminal sacrifice
necropsy status
:planned terminal
date of start of treatment: 06/10/98
date of end of treatment: 02/11/98
date of necropsy :03/11/98
No findings noted
Inflammatory cell foci (slight) Hematopoietic cell foci (minimal)
Hemopoiesis, primarily erythropoiesis (slight)
Plasmacytosis (slight)
Plasmacytosis (slight)
No abnormalities found din: Pancreas, Kidney/1, Kidney/2, Testes/l, Testes/2, Adrenal Gland/1, Adrenal Gland/2, Thymus, Mesenteric Lymph Node.
days of treatment : 28
sacrifice group
: terminal sacrifice
necropsy status
:planned terminal
date of start of treatment: 06/10/98
date of end of treatment: 02/11/98
date of necropsy : 03/11/98
No findings noted
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PATHOLOGY REPORT
Page 47/146
Test Article: t e lo m e r a l c o h o l / te l o m e r a c r y l a t e /
' pehs/ pfos
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. :
242933
Propath no.:
99010
Date
: 21.Apr.1999
Individual Animal Data Records
Animal No: SEX: DOSE GROUP:
2 Male Vehicle Control
MICROSCOPIC FINDINGS
Liver
Testes/1 Testes/2 Spleen
Mandibular Lymph Node/ 1
Animal No:
3
MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS Liver
Pancreas Spleen
Mandibular Lymph Node/ 1
Inflammatory cell foci (slight) Single cell necrosis (minimal)
Seminiferous tubular atrophy (minimal)
Spermatidic giant cell(s) (minimal)
Hemopoiesis, primarily erythropoiesis (slight) Hemosiderin pigment (minimal)
Plasmacytosis (slight) Lymphoid hyperplasia (slight)
No abnormalities found in: Pancreas, Kidney/1, Kidney/2, Adrenal Gland/1, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/2.
days of treatment :28
sacrifice group
:terminal sacrifice
necropsy status
:planned terminal
date of start of treatment: 06/10/98
date of end of treatment: 02/11/98
date of necropsy :03/11/98
No findings noted
Inflammatory cell foci (slight) Single cell necrosis (minimal) Hematopoietic cell foci (minimal)
Apoptosis/single cell necrosis, exocrine (minimal)
Hemopoiesis, primarily erythropoiesis (slight) Hemosiderin pigment (minimal)
Plasmacytosis (slight) Lymphoid hyperplasia (slight)
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PATHOLOGY REPORT
Page 48/146
Teat Article: telomer alcohol/telomer acrylate/ 'pfhs/pfos
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. Propath no. Date
242933 99010
21.A p r . 1999
Individual Animal Data Records
Animal No: SEX: DOSE GROUP:
3 Male Vehicle Control
MICROSCOPIC FINDINGS (continued)
Mandibular Lymph Node/ 2
Animal No:
4
MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS Liver
Kidney/1 Spleen
Mandibular Lymph Node/ 1
Plasmacytosis (slight)
No abnormalities found in: Kidney/1, Kidney/2, Testes/1, Testes/2, Adrenal Gland/1, Adrenal Gland/ 2, Thymus, Mesenteric Lymph Node.
days of treatment : 28
sacrifice group
: terminal sacrifice
necropsy status
:planned terminiti
date of start of treatment: 06/10/98
date of end of treatment: 02/11/98
date of necropsy : 03/11/98
No findings noted
Inflammatory cell foci (slight) Single cell necrosis (minimal)
Tubular atrophy, cortical (minimal)
Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (minimal)
Plasmacytosis (slight)
No abnormalities found in: Pancreas, Kidney/2, Testes/1, Testes/2, Adrenal Gland/1, Adrenal Gland/ 2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/2.
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PATHOLOGY REPORT
Page 49/146
Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ ./PFHS/PFOS
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. Frbpath no. Date
242933 99010
21.Apr.1999
Individual Animal Data Records
Animal No: SEX:
DOSE GROUP:
5
M ale V e h ic le
C o n tro l
MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS Liver
Pancreas Kidney/1 Spleen
Animal No:
6
MACROSCOPIC FINDINGS
days of treatment : 29 sacrifice group : terminal sacrifice necropsy status : planned terminal date of start of treatment: 06/10/98 date of end of treatment: 03/11/98 date of necropsy :04/11/98
No findings noted
Inflammatory cell foci (minimal) Single cell necrosis (minimal)
Apoptosis/single cell necrosis, exocrine (slight)
Interstitial inflammation, lymphocytic (minimal)
Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (slight)
No abnormalities found in: Kidney/2, Testes/l, Testes/2, Adrenal Gland/1, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2.
days of treatment :29
sacrifice group
:terminal sacrifice
necropsy status
:planned terminal
date of start of treatment: 06/10/98
date of end of treatment: 03/11/98
date of necropsy :04/11/98
No findings noted
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PATHOLOGY REPORT
Page 50/146
Test Article: telomer alcohol/telomer acrylate/ /pfhs/pfos
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. Propath no. Date
242933 ' 99010 21.Apr.1999
Individual Animal Data Records
Animal No: SEX: DOSE GROUP:
6 Male Vehicle Control
MICROSCOPIC FINDINGS
Liver
Kidney/1
Kidney/2 Spleen
Mandibular Lymph Node/ 1
Mandibular Lymph Node/ 2
Animal No:
7
MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS Liver
Kidney/1
Inflammatory cell foci (minimal) Single cell necrosis (minimal)
Tubular atrophy, cortical (minimal) Interstitial inflammation, lymphocytic (minimal)
Tubular atrophy, cortical (minimal)
Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (slight)
Plasmacytosis (moderate)
Plasmacytosis (moderate)
No abnormalities found in: Pancreas, Testes/l, Testes/2, Adrenal Gland/1, Adrenal Gland/2, Thymus, Mesenteric Lymph Node.
days of treatment :29
sacrifice group
:terminal sacrifice
necropsy status
:planned terminal
date of start of treatment: 06/10/98
date of end of treatment: 03/11/98
date of necropsy :04/11/98
No findings noted
Inflammatory cell foci (slight) Single cell necrosis (slight) Hepatocellular vacuolation (minimal) Hematopoietic cell foci (minimal)
Hyaline cast(s) (minimal)
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Page 51/146
Test Article: telomer alcohol/telomer acrylate/ 'pfhs/pfos
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. Propath no. Date
242933 9901
21.A p r . 1999
Individual Animal Data Records
Animal Mo: SEX: DOSE GROUP:
7 Male
V e h ic le C o n tro l
MICROSCOPIC FINDINGS (continued)
Spleen
Animal No:
8
MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS Liver KLdney/1 Kidney/2 Spleen
Hemopoiesis, primarily erythropoiesis (slight) Hemosiderin pigment (minimal)
No abnormalities found in: Pancreas, Kidney/2, Testes/l, Testes/2, Adrenal Gland/1, Adrenal Gland/ 2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2.
days of treatment :29
sacrifice group
:terminal sacrifice
necropsy status
:planned terminal
date of start of treatment: 06/10/98
date of end of treatment: 03/11/98
date of necropsy :04/11/98
No findings noted
Inflammatory cell foci (slight)
Tubular atrophy, cortical (minimal)
Interstitial inflammation, lymphocytic (minimal)
Hemopoiesis, primarily erythropoiesis (minimal)
No abnormalities found in: Pancreas, Testes/l, Testes/2, Adrenal Gland/1, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2.
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FATHOLOGX REPORT
Page 52/146
Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PFOS
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. :
242933
Propath no. :
99010
Date
: 21.Apr.1999
Individual Animal Data Records
Animal No: SEX: DOSE GROUP:
9 Male Vehicle Control
MACROSCOPIC FINDINGS
Skin
MICROSCOPIC FINDINGS Liver
Animal No:
10
MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS Liver
.
days of treatment :29
sacrifice group
:recovery group
necropsy status
:planned recovery
date of start of treatment: 06/10/98
date of end of treatment: 03/11/98
date of necropsy :18/11/98
chest region: Alopecia
No abnormalities were found in any of the other tissues examined
Inflammatory cell foci (slight) No abnormalities found in: Pancreas.
days of treatment :29
sacrifice group
:recovery group
necropsy status
:planned recovery
date of start of treatment: 06/10/98
date of end of treatment: 03/11/98
date of necropsy :18/11/98
No findings noted
Inflammatory cell foci (moderate) Single cell necrosis (slight)
No abnormalities found in: Pancreas.
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PATHOLOGE REPORT
Page 53/146
Test Article: telomer alcohol/t'elomer acrylate/ /pfhs/pfos
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. Prbpath no. Date
242933 99010
21.Apr.1999
Individual Animal Data Records
Animal No: SEX: DOSE GROUP:
11 Male Vehicle Control
MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS Liver
Animal No:
13
MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS Liver
.
days of treatment : 29
sacrifice group
: recovery group
necropsy status
: planned recovery
date of start of treatment: 06/10/98
date of end of treatment: 03/11/98
date of necropsy : 18/11/98
No findings noted
Inflammatory cell foci (slight) Single cell necrosis (minimal) Hepatocellular vacuolation (minimal)
No abnormalities found in: Pancreas.
days of treatment : 29
sacrifice group
: recovery group 2
necropsy status
:planned recovery 2
date of start of treatment: 06/10/98
date of end of treatment: 03/11/98
date of necropsy : 02/12/98
No findings noted
Inflammatory cell foci (slight) Single cell necrosis (minimal) Hepatocellular vacuolation (minimal)
No abnormalities found in: Pancreas.
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PATHOLOGY REPORT
Page 54/146
Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PFOS
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. Propath no. Date
242933
99010 21.A p r . 1999
Individual Animal Data Records
Animal No: SEX: DOSE GROUP:
14 Male Vehicle Control
MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS Liver
Animal No:
IS
MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS Liver Pancreas
days of treatment :29
sacrifice group
:recovery group 2
necropsy status
:planned recovery 2
date of start of treatment: 06/10/98
date of end of treatment: 03/11/98
date of necropsy :02/12/98
No findings noted
Inflammatory cell foci (slight) Single cell necrosis (minimal)
No abnormalities found in: Pancreas.
days of treatment : 29
sacrifice group
: recovery group 2
necropsy status
:planned recovery 2
date of start of treatment: 06/10/98
date of end of treatment: 03/11/98
date of necropsy : 02/12/98
No findings noted
Inflammatory cell foci (slight) Apoptosis/single cell necrosis, exocrine (minimal)
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PATHOLOGY REPORT
Page 55/146
Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ 7PFHS/PFOS
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. Propath no. Date
242933 99010
21.Apr.1999
Individual Animal Data Records
Animal No: SEX: DOSE GROUP:
97
F em ale V e h ic le C o n tro l
M ACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS Liver
Kidney/1 Spleen
.
Animal No:
98 .
MACROSCOPIC FINDINGS
Ovaries
days of treatment : 28
sacrifice group
: terminal sacrifice
necropsy status
:planned terminal
date of start of treatment: 08/10/98
date of end of treatment: 04/11/98
date of necropsy : 05/11/98
No findings noted
Inflammatory cell foci (slight) Single cell necrosis (minimal)
Tubular atrophy, cortical (minimal)
Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (moderate)
No abnormalities found in: Pancreas, Kidney/2, Ovary/l, Ovary/2, Adrenal Gland/1, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2.
,
days of treatment :28
sacrifice group
:terminal sacrifice
necropsy status
:planned terminal
date of start of treatment: 08/10/98
date of end of treatment: 04/11/98
date of necropsy :05/11/98
left side Watery cyst
No abnormalities were found in any of the other tissues examined
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Page 56/146
Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PFOS
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. :
242933
Propath no. :
99010
Date
: 21.Apr.1999
Individual Animal Data Records
Animal No: SEX: DOSE GROUP:
98 Female Vehicle Control
MICROSCOPIC FINDINGS
Liver
Pancreas
Kidney/1
Ovary/1
Spleen
Animal No:
99
MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS Liver
Pancreas Spleen
Inflammatory cell foci (minimal)
Apoptosis/single cell necrosis, exocrine (minimal)
Pelvic mineralization (minimal)
No micro correlate
Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (slight)
No abnormalities found in: Kidney/2, Ovary/2, Adrenal Gland/1, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2.
days of treatment : 28
sacrifice group
: terminal sacrifice
necropsy status ;planned terminal
date of start of treatment: 08/10/98
date of end of treatment: 04/11/98
date of necropsy : 05/11/98
No findings noted
Inflammatory cell foci (slight) Single cell necrosis (minimal)
Apoptosis/single cell necrosis, exocrine (minimal)
Hemopoiesis, primarily erythropoiesis (minimal)
Hemosiderin pigment (slight)
'
No abnormalities found in: Kidney/1, Kidney/2, Ovary/1, Ovary/2, Adrenal Gland/1, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2.
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Page 57/146
Test Article: telomer alcohol/telomer acrylate/ ,/pfhs/pfos
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. Propath no. Date
242933 99010
21.Apr.1999
Individual Animal Data Records
Animal No: SEX: DOSE GROUP:
100 Female Vehicle Control
MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS Liver
Kidney/1 Spleen
Animal No:
101
MACROSCOPIC FINDINGS
days of treatment : 28
sacrifice group
: terminal sacrifice
necropsy status
:planned terminal
date of start of treatment: 08/10/98
date of end of treatment: 04/11/98
date of necropsy : 05/11/98
No findings noted
Inflammatory cell foci (minimal) Single cell necrosis (minimal)
Tubular atrophy, cortical (minimal)
Hemopoiesis, primarily erythropoiesis (minimal) ' Hemosiderin pigment (slight)
No abnormalities found in: Pancreas, Kidney/2, Ovary/1, Ovary/2, Adrenal Gland/1, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2.
days of treatment : 29
sacrifice group
: terminal sacrifice
necropsy status
:planned terminal
date of start of treatment: 08/10/98
date of end of treatment: 05/11/98
date of necropsy : 06/11/98 .
No findings noted
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Page 58/146
Test Article: telomer alcohol/t'elomer acrylate/ /pfhs/pfos
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. :
242933
Propath no.:
99010
Date
: 21.Apr.1999
Individual Animal Data Records
Animal No: SEX: DOSE GROUP:
101 Female Vehicle Control
MICROSCOPIC FINDINGS
Liver
K id n ey /1
S p le e n
M a n d ib u la r Lym ph N ode/
1
Animal No:
102
MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS Liver
Kidney/1 Spleen
Inflammatory cell foci (slight) Single cell necrosis (minimal)
Tubular mineralization (minimal)
Hemosiderin pigment (moderate)
Lymphoid hyperplasia (slight)
No abnormalities found in: Pancreas, Kidney/2, Ovary/1, Ovary/2, Adrenal Gland/1, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/2.
days of treatment :29
sacrifice group
:terminal sacrifice
necropsy status
:planned terminal
date of start of treatment: 08/10/98
date of end of treatment: 05/11/98
date of necropsy :06/11/98
No findings noted
Inflammatory cell foci (slight) Single cell necrosis (minimal)
Tubular atrophy, cortical (minimal)
Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (moderate)
No abnormalities found in: Pancreas, Kidney/2, Ovary/1, Ovary/2, Adrenal Gland/1, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2.
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Page 59/146
Test Article: telomer alcohol/telcmer acrylate/ /pfhs/pfos
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. Prpath no. Date
242933 99010
21.Apr.1999
Individual Animal Data Records
Animal No: SEX: DOSE GROUP:
103
F em ale V e h ic le C o n tro l
MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS Liver Spleen Thymus
Animal No:
104
MACROSCOPIC FINDINGS
days of treatment : 29
sacrifice group
: terminal sacrifice
necropsy status
:planned terminal
date of start of treatment: 08/10/98
date of end of treatment: 05/11/98
date of necropsy : 06/11/98
No findings noted
Inflammatory cell foci (slight) Single cell necrosis (minimal)
Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (slight)
Lymphoid atrophy - involution (minimal)
No abnormalities found in: Pancreas, Kidney/1, Kidney/2, Ovary/1, Ovary/2, Adrenal Gland/1, Adrenal Gland/2, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2.
days of treatment : 29
sacrifice group
: terminal sacrifice
necropsy status
:planned terminal
date of start of treatment: 08/10/98
date of end of treatment: 05/11/98
date of necropsy : 06/11/98
No findings noted
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PATHOLOGY REPORT
Page 60/146
Test Article: telomer alcohol/telomer acrylate/ /pfhs/pfos
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. Propath no. Date
242933 99010
21.Apr.1999
Individual Animal Data Records
Animal Ho: SEX: DOSE GROUP:
104 Female Vehicle Control
MICROSCOPIC FINDINGS
Liver
Kidney/1 Spleen
.
Animal No: 105 MACROSCOPIC FINDINGS
Skin
MICROSCOPIC FINDINGS Liver Skin
.
Inflammatory cell foci (minimal) Single cell necrosis (minimal) Hepatocellular vacuolation (minimal) Tubular atrophy, cortical (minimal) Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (slight)
No abnormalities found in: Pancreas, Kidney/2, Ovary/1, Ovary/2, Adrenal Gland/1, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2.
days of treatment : 29 sacrifice group : recovery group necropsy status : planned recovery date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy : 20/11/98
abdominal region: Scab formation No abnormalities were found in any of the other
tissues examined
Inflammatory cell foci (slight) Single cell necrosis (minimal) Inflammation, exudative (slight) (correlates to
GROSS finding)
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Page 61/146
Test Article: telomer alcohol/telomer acrylate/ /pfhs/pfos
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. Propath no. Date
242933 99010
21.Apr.1999
Individual Animal Data Records
Animal Ho: SEX: DOSE GROUP:
106 Female Vehicle Control
MACROSCOPIC FINDINGS
.
MICROSCOPIC FINDINGS Liver
Animal No:
107
MACROSCOPIC FINDINGS
(
'
MICROSCOPIC FINDINGS
Liver
Animal No:
109
MACROSCOPIC FINDINGS
days of treatment : 29
sacrifice group
: recovery group
necropsy status : planned recovery
date of start of treatment: 08/10/98
date of end of treatment: 05/11/98
date of necropsy : 20/11/98
No findings noted
Inflammatory cell foci (slight) Single cell necrosis (minimal) Hepatocellular vacuolation (slight)
days of treatment : 29
sacrifice group
: recovery group
necropsy status : planned recovery
date of start of treatment: 08/10/98
date of end of treatment: 05/11/98
date of necropsy : 20/11/98
No findings noted
Inflammatory cell foci (slight)
days of treatment : 29
sacrifice group
: recovery group 2
necropsy status : planned recovery 2
date of start of treatment: 08/10/98
date of end of treatment: 05/11/98
date of necropsy : 04/12/98
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Page 62/146
Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ ,/PFHS/PFOS
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. Prbpath no. Date
242933 99010
21.Apr.1999
Individual Animal Data Records
Animal No: SEX: DOSE GROUP:
109 Female Vehicle Control
MACROSCOPIC FINDINGS {continued)
MICROSCOPIC FINDINGS Liver
Animal No: 110 MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS Liver
Animal No: 111 MACROSCOPIC FINDINGS
.
MICROSCOPIC FINDINGS Liver
No findings noted
.
Inflammatory cell foci (slight) Single cell necrosis (minimal)
days of treatment : 29 sacrifice group : recovery group 2 necropsy status : planned recovery 2 date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy : 04/12/98
No findings noted
Inflammatory cell foci (slight)
days of treatment : 29
sacrifice group
: recovery group 2
necropsy status : planned recovery 2
date of start of treatment: 08/10/98
date of end of treatment: 05/11/98
date of necropsy : 04/12/98
No findings noted
Inflammatory cell foci (minimal)
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Page 63/146
Test Article: telomer alcohol/telomer acrylate/ /pfhs/pfos
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. Propath no. Date
242933 99010
21.A p r . 1999
Individual Animal Data Records
Animal Ko: SEX: DOSE GROUP:
17 Male Telomer Alcohol
MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS Liver
Pancreas Kidney/1 KLdney/2 Spleen Mandibular Lymph Node/
1
.
Animal No:
18
MACROSCOPIC FINDINGS
days of treatment :28
sacrifice group
:terminal sacrifice
necropsy status
:planned terminal
date of start of treatment: 06/10/98
date of end of treatment: 02/11/98
date of necropsy :03/11/98
No findings noted
Inflammatory cell foci (minimal) Single cell necrosis (minimal)
Apoptosis/single cell necrosis, exocrine (slight)
Tubular atrophy, cortical (slight)
Tubular atrophy, cortical (minimal) Hemopoiesis, primarily erythropoiesis (minimal)
Plasmacytosis (slight)
No abnormalities found in: Testes/l, Testes/2, Adrenal Gland/1, Adrenal Gland/2, Thymus, Mesenteric lymph Node, Mandibular Lymph Node/2.
days of treatment :28
sacrifice group
:terminal sacrifice
necropsy status
:planned terminal
date of start of treatment: 06/10/98
date of end of treatment: 02/11/98 .
date of necropsy :03/11/98
No findings noted
.
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Page 64/146
Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ ,/PFHS/PFOS ' NOTOX no.
Test System : Exploratory 28-Day Gavage Study in Rats Prpath no.
Sponsor
: 3M Toxicology Services
Date
242933 99010
21.Apr.1999
Individual Animal Data Records
Animal No: SEX: DOSE GROUP:
18 Male Telomer Alcohol
MICROSCOPIC FINDINGS
liver
Pancreas Kidney/1
Kidney/2 Spleen
Mandibular Lymph Node/ 1 .
Animal No:
19
MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS Liver
Spleen
Inflammatory cell foci (slight) Midzonal/centrilobular hypertrophy, diffuse
(minimal)
Apoptosis/single cell necrosis, exocrine (minimal)
Tubular atrophy, cortical (minimal) Pelvic mineralization (minimal)
Tubular mineralization (minimal)
Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (minimal)
Plasmacytosis (moderate)
No abnormalities found in: Testes/l, Testes/2, Adrenal Gland/1, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/2.
days of treatment :28
sacrifice group
:terminal sacrifice
necropsy status
:planned terminal
date of start of treatment: 06/10/98
date of end of treatment: 02/11/98
date of necropsy :03/11/98
No findings noted
Inflammatory cell foci (minimal) Midzonal/centrilobular hypertrophy, diffuse
(minimal)
Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (minimal)
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Page 65/146
Test Article: telomer alcohol/telomer acrylate/ /pfhs/pfos
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. Propath no. Date
242933 99010
21.Apr.1999
Individual Animal Data Records
Animal No: SEX: DOSE GROUP:
19 Hale Telamer Alcohol
MICROSCOPIC FINDINGS (continued)
Mesenteric lymph Node
Mandibular Lymph Node/ 1
Animal No:
20
MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS Liver
Pancreas Kidney/1 Spleen
Congestion/erythrophagocytosis (minimal)
Plasmacytosis (slight)
No abnormalities found in: Pancreas, Kidney/1, Kidney/2, Testes/1, Testes/2, Adrenal Gland/1, Adrenal Gland/2, Thymus, Mandibular Lymph Node/2.
days of treatment : 28
sacrifice group
: terminal sacrifice
necropsy status
:planned terminal
date of start of treatment: 06/10/98
date of end of treatment: 02/11/98
date of necropsy : 03/11/98
No findings noted
Inflammatory cell foci (slight) Single cell necrosis (minimal) Midzonal/centrilobular hypertrophy, diffuse
(minimal)
Apoptosis/single cell necrosis, exocrine (minimal)
Tubular atrophy, cortical (minimal)
Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (slight)
No abnormalities found in: Kidney/2, Testes/l, Testes/2, Adrenal Gland/1, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2.
r
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Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PFOS
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. Propath no. Date
242933 99010
2 1 .Apr.1999
Individual Animal Data Records
Animal No: SEX: DOSE GROUP:
21 Hale Telomer Alcohol
MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS Liver
Kidney/1 Kidney/2 Spleen Mandibular Lymph Node/
1
Animal No:
22
MACROSCOPIC FINDINGS
days of treatment : 29
sacrifice group
: terminal sacrifice
necropsy status
:planned terminal
date of start of treatment: 06/10/98
date of end of treatment: 03/11/98
date of necropsy : 04/11/98
No findings noted
Inflammatory cell foci (slight) Single cell necrosis (minimal) Midzonal/centrilobular hypertrophy, diffuse
(minimal)
Interstitial inflammation, lymphocytic (minimal) Hyaline cast(s) (minimal)
Tubular atrophy, cortical (minimal) Interstitial inflammation, lymphocytic (minimal)
Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (minimal)
Plasmacytosis (slight)
' No abnormalities found in: Pancreas, Testes/l, Testes/2, Adrenal Gland/1, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/2.
.
days of treatment : 29
sacrifice group
: terminal sacrifice
necropsy status
: planned terminal
date of start of treatment: 06/10/98
date of end of treatment: 03/11/98
date of necropsy : 04/11/98
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PATHOLOGY REPORT
Test Article: telomer alcohol/telomer acrylate/ /pfhs/pfos
Sponsor
: 3M Toxicology Services
Page 67/146
NOTOX no. Propath no. Date
242933 99010
21.Apr.1999
Individual Animal Data Records
Animal No: SEX: DOSE GROUP:
22 Male Telomer Alcohol
MACROSCOPIC FINDINGS (continued)
MICROSCOPIC FINDINGS Liver
Pancreas Kidney/1 Kidney/2 Spleen
.
Animal No:
23
MACROSCOPIC FINDINGS
No findings ;
Inflammatory cell foci (slight) Single cell necrosis (minimal) Midzonal/centrilobular hypertrophy, diffuse (slight) Apoptosis/single cell necrosis, exocrine (slight) Tubular atrophy, cortical (minimal) Tubular atrophy, cortical (minimal) Hemopoiesis, primarily erythropoiesis (slight) Hemosiderin pigment (minimal) No abnormalities found in: Testes/1, Testes/2, Adrenal Gland/1, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2.
days of treatment : 29
sacrifice group
: terminal sacrifice
necropsy status
: planned terminal
date of start of treatment: 06/10/98
date of end of treatment: 03/11/98
date of necropsy : 04/11/98
No findings noted
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Page 68/146
Test Article: telomer alcohol/telomer acrylate/ ,/pfhs/pfos
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. Prpath no. Date
242933 99010
21.Apr.1999
Individual Animal Data Records
Animal No: SEX: COSE GROUP:
23 Hale Telomer Alcohol
MICROSCOPIC FINDINGS
liver
Pancreas Spleen
Mandibular Lymph Node/ 1
Animal No:
24
MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS liver
Pancreas Spleen
Inflammatory cell foci (slight) Single cell necrosis (minimal)
'
Apoptosis/single cell necrosis, exocrine (minimal)
Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (minimal)
Plasmacytosis (slight)
No abnormalities found in: Kidney/1, Kidney/2, Testes/l, Testes/2, Adrenal Gland/1, Adrenal Gland/ 2, Thymus, Mesenteric Lymph Node, Mandibular lymph Node/2.
days of treatment : 29
sacrifice group
: terminal sacrifice
necropsy status
:planned terminal
date of start of treatment: 06/10/98
date of end of treatment: 03/11/98
date of necropsy : 04/11/98
No findings noted
Inflammatory cell foci (slight)
Single cell necrosis (minimal)
Midzonal/centrilobular hypertrophy, diffuse
(minimal)
.
Apoptosis/single cell necrosis, exocrine (minimal) Exocrine atrophy, focal (minimal)
Hemopoiesis, primarily erythropoiesis (slight) Hemosiderin pigment (minimal)
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PATHOLOGY REPORT
Page 69/146
Test Article: telomer alcohol/telomer acrylate/ /pfhs/pfos
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. Propath no. Date
242933 ' 99010 21.Apr.1999
Individual Animal Data Records
Animal No: SEX: DOSE GROUP:
24 . Hale Telomer Alcohol
MICROSCOPIC FINDINGS (continued)
Mandibular Lymph Node/ 1
'
Animal No:
25
MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS Liver
Animal No:
26
MACROSCOPIC FINDINGS
.. '
Plasmacytosis (slight)
No abnormalities found in: Kldney/1, Kidney/2, Testes/l, Testes/2, Adrenal Gland/1, Adrenal Gland/ 2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/2.
.
days of treatment : 29
.
sacrifice group
:recovery group
necropsy status
:planned recovery
date of start of treatment: 06/10/98
date of end of treatment: 03/11/98
date of necropsy :18/11/98
No findings noted
Inflammatory cell foci (slight) Single cell necrosis (minimal) Hematopoietic cell foci (minimal)
No a b n o r m a litie s fo u n d in : P a n c re a s.
days of treatment :29
sacrifice group
:recovery group
necropsy status
:planned recovery
date of start of treatment: 06/10/98
date of end of treatment: 03/11/98
date of necropsy :18/1/98
No findings noted
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Page 70/146
Test Article: telomer alcohol/telomer acrylate/ /pfhs/pfos
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. :
242933
Propath n o .:
99010'
Date
: 21.Apr.1999
Individual Animal Data Records
Animal No: SEX: DOSE GROUP:
26 Hale Telomer Alcohol
MICROSCOPIC FINDINGS
Liver
Animal No: 27 MACROSCOPIC FINDINGS
.
Testes
MICROSCOPIC FINDINGS Liver Pancreas Testes/l Testes/2
.
Inflammatory cell foci (moderate) Single cell necrosis (minimal) Midzonal/centrilobular hypertrophy, diffuse
(minimal) No abnormalities found in: Pancreas.
days of treatment : 29
sacrifice group
: recovery group
necropsy status
: planned recovery
date of start of treatment: 06/10/98
date of end of treatment: 03/11/98
date of necropsy : 18/11/98
Reduced in size FLACCID
No abnormalities were found in any of the other tissues examined
Inflammatory cell foci (moderate) Single cell necrosis (minimal)
Exocrine atrophy, focal (minimal)
Seminiferous tubular atrophy (severe) (correlates to GROSS finding)
Seminiferous tubular atrophy (severe) (correlates to GROSS finding)
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Page 71/146
Test Article: telomer alcohol/telomer acrylate/ /pfhs/pfos
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. Prbpath. no. Date
242933 99010
21.A p r . 1999
Individual Animal Data Records
Animal No: SEX: DOSE GROUP:
29 Male Telomer Alcohol
MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS Liver
Animal No:
30
MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS Liver
'
.
days of treatment : 29
sacrifice group
: recovery group 2
necropsy status : planned recovery 2
date of start of treatment: 06/10/98
date of end of treatment: 03/11/98
date of necropsy : 02/12/98
No findings noted
Inflammatory cell foci (slight) Midzonal/centrilobular hypertrophy, diffuse
(minimal)
No abnormalities found in: Pancreas.
days of treatment : 29
sacrifice group
: recovery group 2
necropsy status
:planned recovery 2
date of start of treatment: 06/10/98
date of end of treatment: 03/11/98
date of necropsy : 02/12/98
No findings noted
Inflammatory cell foci (slight) Single cell necrosis (minimal) Coagulative necrosis, focal (minimal)
No abnormalities found in: Pancreas.
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Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ ~ f'PFHS/PFOS
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. Propath no. Date
242933 99010
21.Apr.1999
Individual Animal Data Records
Animal No: SEX: DOSE GROUP:
31 Male Telomer Alcohol
MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS Liver
Animal No: SEX:
113 Female
MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS Liver
Spleen
Mandibular Lymph Node/ 1
days of treatment : 29
sacrifice group
: recovery group 2
necropsy status
:planned recovery 2
date of start of treatment: 06/10/98
date of end of treatment: 03/11/98
date of necropsy : 02/12/98
No findings noted
Inflammatory cell foci (slight) Single cell necrosis (minimal)
No abnormalities found in: Pancreas.
days of treatment : 28
sacrifice group
: terminal sacrifice
necropsy status
:planned terminal
date of start of treatment: 08/10/98
date of end of treatment: 04/11/98
date of necropsy : 05/11/98
No findings noted
Inflammatory cell foci (slight) Single cell necrosis (minimal)
Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (slight)
Plasmacytosis (slight) Lymphoid hyperplasia (slight)
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Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PEOS
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. Propath no. Date
242933 99010
21.Apr.1999
Individual Animal Data Records
Animal No: SEX: DOSE GROUP:
113 Female Telamer Alcohol
MICROSCOPIC FINDINGS (continued)
Animal No: 114 MACROSCOPIC FINDINGS
'
MICROSCOPIC FINDINGS Liver
Spleen
Mandibular Lymph Node/ 1'
-
No abnormalities found in: Pancreas, Kidney/1, Kidney/2, Ovary/1, Ovary/2, Adrenal Gland/1, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/2.
days of treatment : 28
sacrifice group
: terminal sacrifice
necropsy status
: planned terminal
date of start of treatment: 08/10/98
date of end of treatment: 04/11/98
date of necropsy : 05/11/98
No findings noted
Inflammatory cell foci (slight) Single cell necrosis (minimal)
Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (slight)
Plasmacytosis (slight)
No abnormalities found in: Pancreas, Kidney/1, Kidney/2, Ovary/1, Ovary/2, Adrenal Gland/1, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/2.
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Test Article: telomer alcohol/telomer acrylate/ ./pfhs/pfos
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. Pr'opath no. Date
242933 99010
21.Apr.1999
Individual Animal Data Records
Animal No: SEX: DOSE GROUP:
115
Female Telomer Alcohol
MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS liver Spleen
Animal No:
116
MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS Liver
Spleen
days of treatment : 28
sacrifice group : terminal sacrifice
necropsy status
: planned terminal
date of start of treatment: 08/10/98
date of end of treatment: 04/11/98
date of necropsy : 05/11/98
No findings noted
Inflammatory cell foci (minimal)
Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (slight)
No abnormalities found in: Pancreas, KLdney/1, Kidney/2, Ovary/1, Ovary/2, Adrenal Gland/1, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2.
days of treatment :28 .
sacrifice group
:terminal sacrifice
necropsy status
:planned terminal
date of start of treatment: 08/10/98
date of end of treatment: 04/11/98
date of necropsy :05/11/98
No findings noted
Inflammatory cell foci (minimal) Single cell necrosis (minimal)
Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (slight)
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Page 75/146
Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PFOS
Test System ; Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. Propath no. Date
242933 99010
21.Apr.1999
Individual Animal Data Records
Animal No: SEX: DOSE GROUP:
116 Female Telomer Alcohol
MICROSCOPIC FINDINGS (continued)
Animal No: 117 MACROSCOPIC FINDINGS
Kidneys
MICROSCOPIC FINDINGS Liver Kidney/2
Adrenal Gland/1 Spleen
. .
-
No abnormalities found in: Pancreas, Kidney/1, Kidney/2, Ovary/1, Ovary/2, Adrenal Gland/1, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2.
.
days of treatment : 29
sacrifice group
: terminal sacrifice
necropsy status
:planned terminal
date of start of treatment: 08/10/98
date of end of treatment: 05/11/98
date of necropsy : 06/11/98
right side Pelvic dilation
No abnormalities vere found in any of the other tissues examined
Inflammatory cell foci (minimal)
Pelvic dilation present (correlatesto GROSS finding)
Extracapsular nodule present
Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (slight)
No abnormalities found in: Pancreas, Kidney/1, Ovary/1, Ovary/2, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/1,
' Mandibular Lymph Node/2.. .
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Page 76/146
Test Article: telcmer alcohol/telomer acrylate/ ,/pfhs/pfos
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. :
242933
Pr'opath no.:
99010
Date
: 21.Apr.1999
Individual Animal Data Records
Animal No: SEX: DOSE GROUP:
118 Female Telomer Alcohol
MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS Liver
Spleen
Thymus Mandibular Lymph Node/
1
Animal No:
119
MACROSCOPIC FINDINGS
days of treatment :29
sacrifice group
:terminal sacrifice
necropsy status
:planned terminal
date of start of treatment: 08/10/98
date of end of treatment: 05/11/98
date-of necropsy :06/11/98
No findings noted
Inflammatory cell foci (minimal) Single cell necrosis (minimal)
Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (slight)
Lymphoid atrophy - involution (minimal)
Plasmacytosis (moderate)
No abnormalities found in: Pancreas, Kidney/1, Kidney/2, Ovary/1, Ovary/2, Adrenal Gland/1, Adrenal Gland/2, Mesenteric Lymph Node, Mandibular Lymph Node/2.
days of treatment : 29
sacrifice group
: terminal sacrifice
necropsy status
:planned terminal
date of start of treatment: 08/10/98
date of end of treatment: 05/11/98
date of necropsy : 06/11/98
No findings noted
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Page 77/146
Test Article: telomer alcohol/telomer acrylate/.. /pfhs/pfos
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. Propath no. Date
242933 99010
21.Apr.1999
Individual Animal Data Records
Animal No: SEX: DOSE GROUP:
119 Female Telomer Alcohol
MICROSCOPIC FINDINGS
Liver
Kidney/2 Spleen
Thymus
Animal No:
120
MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS Liver
Spleen
Inflammatory cell foci (minimal) Single cell necrosis (minimal)
Tubular atrophy, cortical (minimal)
Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (moderate)
Lymphocytolysis, increased (minimal)
No abnormalities found in: Pancreas, Kidney/1, Ovary/1, Ovary/2, Adrenal Gland/1, Adrenal Gland/2 Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2.
days of treatment : 29
sacrifice group
: terminal sacrifice
necropsy status
: planned terminal
date of start of treatment: 08/10/98
date of end of treatment: 05/11/98
date of necropsy : 06/11/98
No findings noted
Inflammatory cell foci (slight) Single cell necrosis (minimal)
Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (slight)
No abnormalities found in: Pancreas, Kidney/1, Kidney/2, Ovary/1, Ovary/2, Adrenal Gland/1, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2.
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Test Article: telomer alcohol/telomer acrylate/ /pfhs/pfos
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. PYopath no. Date
242933 99010
21.Apr.1999
Individual Animal Data Records
Animal No: SEX: DOSE GROUP:
121 Female Telomer Alcohol
MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS
Animal No:
122
MACROSCOPIC FINDINGS
Skin
MICROSCOPIC FINDINGS
Skin
Animal No:
123
MACROSCOPIC FINDINGS
days of treatment : 29
sacrifice group
: recovery group
necropsy status
: planned recovery
date of start of treatment: 08/10/98
date of end of treatment: 05/11/98
date of necropsy : 20/11/98
No findings noted
No tissues examined
days of treatment :29
sacrifice group
:recovery group
necropsy status
:planned recovery
date of start of treatment: 08/10/98
date of end of treatment: 05/11/98
date of necropsy :20/11/98
Alopecia
No abnormalities were found in any of the other tissues examined
No micro correlate
days of treatment : 29
sacrifice group
: recovery group
necropsy status
:planned recovery
date of start of treatment: 08/10/98
date of end of treatment: 05/11/98
date of necropsy : 20/11/98
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Test Article: telomer alcohol/telomer acrylate/ ,/pfhs/pfos
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. Propath no. Date
242933 99010
21.Apr.1999
Individual Animal Data Records
Animal No: SEX: DOSE GROUP:
123 Female Telomer Alcohol
MACROSCOPIC FINDINGS (continued)
MICROSCOPIC FINDINGS
Animal No:
125
MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS
Animal No:
126
MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS
No findings noted No tissues examined
days of treatment : 29
sacrifice group
: recovery group 2
necropsy status
:planned recovery 2
date of start of treatment: 08/10/98
date of end of treatment: 05/11/98
date of necropsy : 04/12/98
No findings noted
No tissues examined
days of treatment : 29
sacrifice group
: recovery group 2
necropsy status
:planned recovery 2
date of start of treatment: 08/10/98
date of end of treatment: 05/11/98
date of necropsy : 04/12/98
No findings noted
No tissues examined
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T e s t A r t i c l e : telomer alcohol/Telomer acrylate/ /pfhs/pfos
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
Page 80/146
NOTOX no. Prbpath no. Date
242933 99010
21.A p r . 1999
Individual Animal Data Records
Animal No: SEX: DOSE GROUP:
127 Female Telamer Alcohol
MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS
Animal No:
SEX: DOSE GROUP:
33
Male Telomer Acrylat
MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS Liver
Pancreas Kidney/1 Kidney/2 Spleen
days of treatment : 29
sacrifice group
: recovery group 2
necropsy status
:planned recovery 2
date of start of treatment: 08/10/98
date of end of treatment: 05/11/98
date of necropsy
: 04/12/98
No findings noted
No tissues examined
days of treatment : 28
sacrifice group
: terminal sacrifice
necropsy status
:planned terminal
date of start of treatment: 06/10/98
date of end of treatment: 02/11/98
date of necropsy
: 03/11/98
No findings noted
Inflammatory cell foci (slight) Single cell necrosis (minimal)
Apoptosis/single cell necrosis, exocrine (minimal)
Tubular atrophy, cortical (minimal)
Tubular atrophy, cortical (minimal)
Hemopoiesis, primarily erythropoiesis (slight) Hemosiderin pigment (minimal)
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T e s t A r t i c l e : TELOMER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PFOS
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
Page 81/146
NOTOX no. :
Prbpath no. :
Date
:
242933 99010
21.A p r . 1999
Individual Animal Data Records
Animal No:
SEX: DOSE GROUP:
33
M ale T elo m er
A c ry la t
M ICROSCOPIC FINDINGS (co n tin u e d )
Animal No:
34
MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS
Liver
Kidney/1
Spleen
Mandibular Lymph Node/ 1
Mandibular Lymph Node/ 2
Number of Sections less than protocol for Mandibular Lymph Node/1 (0).
No abnormalities found in: Testes/1, Testes/2, Adrenal Gland/1, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/2.
days of treatment : 28
sacrifice group
: terminal sacrifice
necropsy status
:planned terminal
date of start of treatment: 06/10/98
date of end of treatment: 02/11/98
date of necropsy
: 03/11/98
No findings noted
Inflammatory cell foci (minimal) Single cell necrosis (minimal) Interstitial inflammation, lymphocytic (minimal)
Hemopoiesis, primarily erythropoiesis (minimal)
Plasmacytosis (slight)
Plasmacytosis (slight)
No abnormalities found in: Pancreas, Kidney/2, Testes/1, Testes/2, Adrenal Gland/1, Adrenal Gland/ 2, Thymus, Mesenteric Lymph Node.
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T e s t A r t i c l e : telomer alcohol/telomer acrylate/ /pfhs/pfos
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. Propath no. Date
242933 99010
21.A p r . 1999
Individual Animal Data Records
A n im al No: SEX: DOSE GROUP:
35
Hale Telomer Acrylat
M ACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS Liver Spleen
Animal No:
36
MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS Liver
K id n ey/1
Spleen
days of treatment : 28
sacrifice group
: terminal sacrifice
necropsy status
:planned terminal
date of start of treatment: 06/10/98
date of end of treatment: 02/11/98
date of necropsy : 03/11/98
No findings noted
Inflammatory cell foci (minimal)
Hemopoiesis, primarily erythropoiesis (minimal)
No abnormalities found in: Pancreas, ICidney/1, Kidney/2, Testes/l, Testes/2, Adrenal Gland/1, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2.
days of treatment : 28
sacrifice group
: terminal sacrifice
necropsy status
:planned terminal
date of start of treatment: 06/10/98
date of end of treatment: 02/11/98
date of necropsy
: 03/11/98
No findings noted
Inflammatory cell foci (slight) Single cell necrosis (minimal)
Tubular atrophy, cortical (minimal) Interstitial inflammation, lymphocytic (minimal)
Hemopoiesis, primarily erythropoiesis (minimal)
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Test Article: telomer alcohol/telomer acrylate/
./pfh s/ pfo s
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. Propath no. Date
242933 99010
2 1 .Apr.1999
Individual Animal Data Records
Animal No:
SEX: DOSE GROUP:
36
M ale T elo m er
A c ry la t
M ICRO SCO PIC FINDINGS
(continued) .
Animal No:
37
M ACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS Liver
Kidney/1 Spleen
Mandibular Lymph Node/ .1
Number of Sections less than protocol for Mandibular Lymph Node/1 (0).
No abnormalities found in: Pancreas, Kidney/2, Testes/1, Testes/2, Adrenal Gland/1, Adrenal Gland/ 2, Thymus, Mesenteric Lymph Node, Mandibular Lym p h Node/2.
days of treatment : 29
sacrifice group
: terminal sacrifice
necropsy status
:planned terminal
date of start of treatment: 06/10/98
date of end of treatment: 03/11/98
date of necropsy
: 04/11/98
No findings noted
Inflammatory cell foci (moderate) Single cell necrosis (minimal)
Tubular atrophy, cortical (minimal)
Hemopoiesis, primarily erythropoiesis (slight) Hemosiderin pigment (minimal)
Plasmacytosis (slight)
No abnormalities found in: Pancreas, Kidney/2, Testes/1, Testes/2, Adrenal Gland/1, Adrenal Gland/ 2, Thymus, Mesenteric Lymph Node, Mandibular Lym p h Node/2.
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PATHOLOGE REPORT
T e s t A r t i c l e : telomer alcohol/telomer acrylate/ /pfhs/pfos
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
Page 84/146
NOTOX no. Propath no. Date
242933 99010
21.Apr.1999
Individual Animal Data Records
Animal No: SEX: DOSE GROUP:
38 Hale Telomer Acrylat
MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS Liver
Spleen
Mandibular Lymph Node/ 1
Mandibular Lymph Node/ 2
Animal No:
39
MACROSCOPIC FINDINGS
days of treatment : 29
sacrifice group
: terminal sacrifice
necropsy status
: planned terminal
date of start of treatment: 06/10/98
date of end of treatment: 03/11/98
date of necropsy
: 04/11/98
No findings noted
Inflammatory cell foci (slight) Single cell necrosis (minimal)
Hemopoiesis, primarily erythropoiesis (slight) Hemosiderin pigment (minimal)
Plasmacytosis (slight)
Plasmacytosis (slight)
No abnormalities found in: Pancreas, Kidney/1, Kidney/2, Testes/l, Testes/2, Adrenal Gland/1, Adrenal Gland/2, Thyznus, Mesenteric Lymph Node.
days of treatment : 29
sacrifice group
: terminal sacrifice
necropsy status
:planned terminal
date of start of treatment: 06/10/98
date of end of treatment: 03/11/98
date of necropsy
: 04/11/98
No findings noted
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Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PFOS
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. Propath no. Date
242933 99010
21.A p r .1999
Individual Animal Data Records
Animal No:
SEX: DOSE GROUP:
39
Hale Telomer Acrylat
MICROSCOPIC FINDINGS
Liver
Pancreas
Kidney/1
Spleen
Animal No:
40
MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS Liver Kidney/1 Spleen
Thymus Mandibular Lymph Node/
1
Inflammatory cell foci (slight) Single cell necrosis (minimal)
Apoptosis/single cell necrosis, exocrine (minimal) Exocrine atrophy, focal (minimal)
Tubular atrophy, cortical (minimal) Pelvic inflammation, lymphoid (minimal)
Hemopoiesis, primarily erythropoiesis (slight) Hemosiderin pigment (minimal)
No abnormalities found in: Kidney/2, Testes/l, Testes/2, Adrenal Gland/1, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2.
days of treatment : 29
sacrifice group
: terminal sacrifice
necropsy status
:planned terminal
date of start of treatment: 06/10/98
date of end of treatment: 03/11/98
date of necropsy
: 04/11/98
No findings noted
Inflammatory cell foci (slight)
Tubular atrophy, cortical (minimal) Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (minimal) Lymphocytolysis, increased (slight) Congestion/erythrophagocytosis (moderate)
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Test Article: te l o m e r a l c o h o l / t e l o m e r a c r y l a t e /
/ pfhs/ pfos
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. Propth no. Date
242933 99010
21.Apr.1999
Individual Animal Data Records
Animal No: SEX: DOSE GROUP:
40 Male Telomer Aerylat
MICROSCOPIC FINDINGS (continued)
Animal No:
41
MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS
Animal No:
42
MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS
-
No abnormalities found in: Pancreas, Kidney/2, Testes/1, Testes/2, Adrenal Gland/1, Adrenal Gland/ 2, Mesenteric Lymph Node, Mandibular Lymph Node/2.
days of treatment :29
sacrifice group
:recovery group
necropsy status
:planned recovery
date of start of treatment: 06/10/98
date of end of treatment: 03/11/98
date of necropsy
:18/11/98
No findings noted
No tissues examined
days of treatment : 29
sacrifice group
: recovery group
necropsy status
:planned recovery
date of start of treatment: 06/10/98
date of end of treatment: 03/11/98
date of necropsy
:18/11/98
No findings noted
No tissues examined
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Page 87/146
Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ ,/PFHS/PFOS
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. Propath no. Date
242933 99010
21.A p r . 1999
Individual Animal Data Records
Animal No: SEX: DOSE GROUP:
43
M ale T elo m er A c r y la t
MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS
Animal No:
45
MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS
Animal No:
46
MACROSCOPIC FINDINGS
days of treatment : 29
sacrifice group
: recovery group
necropsy status
: planned recovery
date of start of treatment: 06/10/98
date of end of treatment: 03/11/98
date of necropsy
: 18/11/98
No findings noted
No tissues examined
days of treatment : 29
sacrifice group
: recovery group 2
necropsy status
:planned recovery 2
date of start of treatment: 06/10/98
date of end of treatment: 03/11/98
date of necropsy
: 02/12/98
No findings noted
No tissues examined
days of treatment : 29
sacrifice group
: recovery group 2
necropsy status
:planned recovery 2
date of start of treatment: 06/10/98
date of end of treatment: 03/11/98
date of necropsy
: 02/12/98
No findings noted
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Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PFOS
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. Propath no. Date
.242933 99010
21.Apr.1999
Individual Animal Data Records
Animal No: SEX: DOSE GROUP:
46 Male Telomer Acrylat
MICROSCOPIC FINDINGS
Animal No:
47
MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS
Animal No: SEX:
129 Female
MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS Liver
Spleen
No tissues examined
days of treatment :29
sacrifice group
:recovery group 2
necropsy status
:planned recovery
date of start of treatment: 06/10/98
date of end of treatment: 03/11/98
date of necropsy
:02/12/98
2
No findings noted
No tissues examined
days of treatment :28
sacrifice group
:terminal sacrifice
necropsy status
:other
date of start of treatment: 08/10/98
date of end of treatment: 04/11/98
date of necropsy
:05/11/98
No findings noted
'
Inflammatory cell foci (slight) Single cell necrosis (minimal)
Hemopoiesis, primarily erythropoiesis (slight) Hemosiderin pigment (minimal)
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Page 89/146
Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PFOS
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. Pfopath no. Date
242933 99010
21.Apr.1999
Individual Animal Data Records
Animal No: SEX: DOSE GROUP:
129 Female
T elo m er A c r y la t
MICROSCOPIC FINDINGS (continued)
Animal No:
130
MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS Liver
KLdney/1 Kidney/2 Spleen
Thymus
No abnormalities found in: Pancreas, Kidney/l, Kidney/2, Ovary/1, Ovary/2, Adrenal Gland/1, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Nod e / 2.
days of treatment : 28
sacrifice group
: terminal sacrifice
necropsy status
:planned terminal
date of start of treatment: 08/10/98
date of end of treatment: 04/11/98
date of necropsy
: 05/11/98
No findings noted
Inflammatory cell foci (slight) Single cell necrosis (minimal) Hepatocellular vacuolation (minimal)
Tubular atrophy, cortical (minimal)
Tubular atrophy, cortical (minimal)
Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (slight)
Lymphoid atrophy - involution (minimal)
No abnormalities found in: Pancreas, Ovary/1, Ovary/ 2, Adrenal Gland/I, Adrenal Gland/2, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2.
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Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ -/PFHS/PFOS
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. Prpath no. Date
242933 99010
21.Apr.1999
I n d iv id u a l A n im a l D a ta R e c o r d s
Animal No: SEX:
DOSE GROUP:
131 Female
T e lo m er A c r y la t
MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS Liver Spleen .
Animal No:
132
MACROSCOPIC FINDINGS
days of treatment :28
sacrifice group
:terminal sacrifice
necropsy status
:planned terminal
date of start of treatment: 08/10/98
date of end of treatment: 04/11/98
date of necropsy
:05/11/98
No findings noted
Inflammatory cell foci (minimal) Single cell necrosis (minimal)
Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (slight)
No abnormalities found in: Pancreas, Kidney/1, Kidney/2, Ovary/1, Ovary/2, Adrenal Gland/1, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lynph Node/1, Mandibular Lymph Node/2.
days of treatment : 28
sacrifice group
: terminal sacrifice
necropsy status
:planned terminal
date of start of treatment: 08/10/98
date of end of treatment: 04/11/98
date of necropsy : 05/11/98
No findings noted
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Page 91/146
Test Article: te l o m e r a l c o h o l / t e l o m e r a c r y l a t e /
,/pfhs/ pfos
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. Propath no. Date
242933 99010
21.Apr.1999
Individual Animal Data Records
Animal No:
SEX:
DOSE GROUP:
132
F em ale
Telomer Acrylat
MICROSCOPIC FINDINGS liver
Pancreas
S p le e n
Animal No:
133
MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS' Liver Spleen Thymus
'
.
Inflammatory cell foci (minimal)
.
Exocrine atrophy, focal (minimal)
H e m o p o ie sis, p r im a r ily e r y t h r o p o ie s is (m in im al)
Hemosiderin pigment (minimal)
No abnormalities found in: Kidney/1, Kidney/2, Ovary/1, Ovary/2, Adrenal Gland/1, Adrenal Gland/2,
Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2.
days of treatment :29
sacrifice group
:terminal sacrifice
necropsy status
:planned terminti
date of start of treatment: 08/10/98
date of end of treatment: 05/11/98
date of necropsy
:06/11/98
No findings noted
Inflammatory cell foci (slight)
S in g le c e ll n e c ro sis (s lig h t)
Hemopoiesis, primarily erythropoiesis (slight) Hemosiderin pigment (slight)
Lymphoid atrophy - involution (minimal)
No abnormalities found in: Pancreas, Kidney/1, Kidney/2, Ovary/1, Ovary/2, Adrenal Gland/1, Adrenal Gland/2, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2.
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Page 92/146
T e s t A r t i c l e : telomer alcohol/telomer acrylate/ /pfhs/pfos
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
N OTOX no. Propath no. Date
242933 99010
21.Apr.1999
Individual Animal Data Records
Animal No: SEX: DOSE GROUP:
134 Female Telomer Acrylat
MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS Liver KLdney/2
Spleen
Thymus
Animal No:
135
MACROSCOPIC FINDINGS
days of treatment : 29
sacrifice group
: terminal sacrifice
necropsy status
: planned terminal
date of start of treatment: 08/10/98
date of end of treatment: 05/11/98
date of necropsy
: 06/11/98
No findings noted
Inflammatory cell foci (minimal)
Tubular atrophy, cortical (minimal) Interstitial inflammation, lymphocytic (minimal)
Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (slight)
Lyraphocytolysis, increased (minimal)
No abnormalities found in: Pancreas, Kidney/1, Ovary/1, Ovary/2, Adrenal Gland/1, Adrenal Gland/2, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2.
days of treatment :29
sacrifice group
:terminal sacrifice
necropsy status
:planned terminal
date of start of treatment: 08/10/98
date of end of treatment: 05/11/98
date of necropsy
:06/11/98
No findings noted
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Page 93/146
Test Article: t e l o m e r a l c o h o l / t e l c m e r a c r y l a t e /
/ pfhs/ pfos
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. Prbpath no. Date
242933 99010
21.A p r . 1999
Individual Animal Data Records
Animal No:
SEX: DOSE GROUP:
135
female Telomer Acrylat
MICROSCOPIC FINDINGS
Liver
Spleen
Mandibular Lymph Node/
1
Animal No:
136
MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS
L iv e r
Kidney/2 Adrenal Gland/1 Spleen
Thymus Mandibular Lymph Node/
1
Inflammatory cell foci (minimal)
Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (slight)
Plasmacytosis (slight) Lymphoid hyperplasia (slight)
No abnormalities found in: Pancreas, Kidney/1, Kidney/2, Ovary/1, Ovary/2, Adrenal Gland/1, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/2.
days of treatment : 29
sacrifice group
: terminal sacrifice
necropsy status
: planned terminal
date of start of treatment: 08/10/98
date of end of treatment: 05/11/98
date of necropsy
: 06/11/98
No findings noted
In fla m m a to ry c e l l f o c i ( s lig h t )
Single cell necrosis (minimal)
Interstitial inflammation, lymphocytic (minimal)
Extracapsular nodule present
Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (moderate)
Lymphoid atrophy - involution (minimal)
Plasmacytosis (slight)
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Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PFOS
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. Prtpath no. Date
242933 99010
21.Apr.1999
Individual Animal Data Records
Animal No: SEX: DOSE GROUP:
136 Female Telamer Acrylat
MICROSCOPIC FINDINGS (continued)
Mandibular Lymph Node/ 2
Animal No:
137
MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS
Animal No:
138
MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS
Lymphoid hyperplasia (slight)
No abnormalities found in: Pancreas, Kidney/1, Ovary/1, Ovary/2, Adrenal Gland/2, Mesenteric Lymph Node.
days of treatment : 29
sacrifice group
: recovery group
necropsy status
: planned recovery
date of start of treatment: 08/10/98
date of end of treatment: 05/11/98
date of necropsy : 20/11/98
No findings noted
No tissues examined
days of treatment : 29
sacrifice group
: recovery group
necropsy status
: planned recovery
date of start of treatment: 08/10/98
date of end of treatment: 05/11/98
date of necropsy : 20/11/98
No findings noted
No tissues examined
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Page 95/146
Test Article: t e l o m e r a l c o h o l / t e l o m e r a c r y l a t e /
/ pfhs/ pfos
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. Propath no. Date
242933 99010
21.Apr.1999
Individual Animal Data Records
Animal No: SEX: DOSE GROUP:
139 Female Telomer Acrylat
MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS
Animal No:
141
MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS
Animal No:
142
MACROSCOPIC FINDINGS
days of treatment : 29
sacrifice group
: recovery group
necropsy status
: planned recovery
date of start of treatment: 08/10/98
date of end of treatment: 05/11/98
date of necropsy
: 20/11/98
No findings noted
No tissues examined
days of treatment : 29
sacrifice group
: recovery group 2
necropsy status
:planned recovery 2
date of start of treatment: 08/10/98
date of end of treatment: 05/11/98
date of necropsy
: 04/12/98
No findings noted
No tissues examined
days of treatment : 29
sacrifice group
: recovery group 2
necropsy status
:planned recovery 2
date of start of treatment: 08/10/98
date of end of treatment: 05/11/98
date of necropsy
: 04/12/98
No findings noted
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Page 96/146
T e s t A r t i c l e : TELOMER ALCOHOL/TELOMER ACRYLATE/ ,/PFHS/PFOS
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. Pfopath no. Date
242933 99010
21.A p r . 1999
Individual Animal Data Records
Animal No: SEX: DOSE GROUP:
142 Female Telomer Acrylat
MICROSCOPIC FINDINGS
Animal No:
143
MACROSCOPIC FINDINGS
M ICROSCOPIC FINDINGS
Animal No: SEX: DOSE GROUP:
49 Male
MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS Liver
Kidney/1 Spleen
No tissues examined
days of treatment : 29
sacrifice group
: recovery group 2
necropsy status
:planned recovery 2
date of start of treatment: 08/10/98
date of end of treatment: 05/11/98
date of necropsy
: 04/12/98
No findings noted
No tissues examined
days of treatment : 28
sacrifice group
: terminal sacrifice
necropsy status
:planned terminal
date of start of treatment: 06/10/98
date of end of treatment: 02/11/98
date of necropsy
: 03/11/98
No findings noted
Inflammatory cell foci (slight) Single cell necrosis (minimal)
Tubular atrophy, cortical (minimal)
Hemopoiesis, primarily erythropoiesis (minimal)
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Page 97/146
Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ .-i/PFHS/EFOS
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. Prpath no. Date
242933 99010
21.Apr.1999
Individual Animal Data Records
Animal No: SEX: DOSE G/ROUP:
49 Hale
.
MICROSCOPIC FINDINGS (continued)
Animal No:
50
MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS Liver
Spleen
Animal No:
51
MACROSCOPIC FINDINGS
.
No abnormalities found in: Pancreas, Kidney/2, Testes/l, Testes/2, Adrenal Gland/1, Adrenal Gland/
2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2.
days of treatment :28
sacrifice group
:terminal sacrifice
necropsy status
:planned terminal
date of start of treatment: 06/10/98
date of end of treatment: 02/11/98
date of necropsy
:03/11/98
No findings noted
Inflammatory cell foci (minimal) Single cell necrosis (minimal)
Hemopoiesis, primarily erythropoiesis (m in im al) Hemosiderin pigment (minimal)
N o abnormalities found in: Pancreas, KLdney/1,
K id n e y /2 , T e s t e s / l, T e ste s/ 2 , A d re n a l G la n d / 1,
Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2.
days of treatment : 28
sacrifice group
: terminali sacrifice
necropsy status
: planned terminal
date of staurt of treatment: 06/10/98 .
date of end of treatment: 02/11/98
date of necropsy
: 03/11/98
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Page 98/146
Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ ,/PFHS/PFOS
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. Propath no. Date
242933 99010
21.Apr.1999
Individual Animal Data Records
Animal No: SEX: DOSE GROUP:
51 Hale
MACROSCOPIC FINDINGS (continued)
MICROSCOPIC FINDINGS Liver Spleen
Animal No:
52
MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS Liver Spleen
Mesenteric Lymph Node Mandibular Lymph Node/
1
Mandibular Lymph Node/ 2
No findings noted
Inflammatory cell foci (minimal) -
Hemopoiesis, primarily erythropoiesis (slight) Hemosiderin pigment (minimal)
No abnormalities found in: Pancreas, Kidney/1, Kidney/2, Testes/l, Testes/2, Adrenal Gland/1, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2.
days of treatment : 28
sacrifice group
: terminal sacrifice
necropsy status
:planned terminal
date of start of treatment: 06/10/98
date of end of treatment: 02/11/98
date of necropsy
: 03/11/98
No findings noted
Inflammatory cell foci (minimal)
Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (minimal)
Congestion/erythrophagocytosis (minimal)
Plasmacytosis (slight) Lymphoid hyperplasia (slight) Congestion/erythrophagocytosis (slight)
Congestion/erythrophagocytosis (slight)
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Te s t Article: telomer alcohol/ telomer acrylate/
/ pfhs/ pfos
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
Page 99/146
NOTOX no. Propath no. Date
242933
99010 2 1 .Apr.1999
Individual Animal Data Records
Animal No: SEX: DOSE GROUP:
52 Male
MICROSCOPIC FINDINGS (continued)
Animal No:
53
MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS Liver Kidney/1
Spleen
Animal No:
54
MACROSCOPIC FINDINGS
.
No abnormalities found in: Pancreas, Kidney/1, Kidney/2, Testes/1, Testes/2; Adrenal Gland/1, Adrenal Gland/2, Thymus.
days of treatment : 29
sacrifice group
: terminal sacrifice
necropsy status
: planned terminal
date of start of treatment: 06/10/98
date of end of treatment: 03/11/98
date of necropsy : 04/11/98
No findings noted
Inflammatory cell foci (slight)
Tubular atrophy, cortical (minimal) Interstitial inflammation, lymphocytic (minimal)
Hemopoiesis, primarily erythropoiesis (slight) Hemosiderin pigment (minimal)
No abnormalities found in: Pancreas, Kidney/2, Testes/1, Testes/2, Adrenal Gland/1, Adrenal Gland/ 2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2.
days of treatment : 29
sacrifice group
: terminal sacrifice
necropsy status
:planned terminal
date of start of treatment: 06/10/98
date of end of treatment: 03/11/98
date of necropsy
: 04/11/98
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Page 100/146
T e s t A r t i c l e : telomer alcohol/telomer acrylate/ /ffhs/pfos
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
N OTOX no. Prpath no. Date
242933 99010
21.Apr.1999
Individual Animal Data Records
Animal No: SEX: DOSE GROUP:
54 Male
MACROSCOPIC FINDINGS (continued)
MICROSCOPIC FINDINGS Liver
KLdney/1 Spleen
Animal No:
55
MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS Liver Pancreas Spleen
Mandibular Lymph Node/ 1
.
No findings noted ' Inflammatory cell foci (slight) Single cell necrosis (minimal) Hyaline cast(s) (minimal) Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (minimal) No abnormalities found in: Pancreas, Kidney/2,
Testes/l, Testes/2, Adrenal Gland/1, Adrenal Gland/ 2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2.
days of treatment :29
sacrifice group
:terminal sacrifice
necropsy status
:planned terminal
date of start of treatment: 06/10/98
date of end of treatment: 03/11/98
date of necropsy
: 04/11/98
No findings noted
Inflammatory cell foci (slight)
Apoptosis/single cell necrosis, exocrine (slight)
Hemopoiesis, primarily erythropoiesis (slight) Hemosiderin pigment (minimal)
Plasmacytosis (slight)
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Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PFOS
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
Page 101/146
NOTOX no. Propath no. Date
242933 99010
21.Apr.1999
Individual Animal Data Records
Animal No: SEX:
DOSE GROUP:
55 Male
MICROSCOPIC FINDINGS (continued)
Mandibular Lymph Node/ 2
Animal No:
56
MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS Liver KLdney/1 Kidney/2
Adrenal Gland/1 Spleen
Thymus
.
Plasmacytosis (slight)
No abnormalities found in: KLdney/1, Kidney/2, Testes/1, Testes/2, Adrenal Gland/1, Adrenal Gland/ 2, Thymus, Mesenteric Lymph Node.
days of treatment : 29
sacrifice group
: terminal sacrifice
necropsy status
:planned terminal
date of start of treatment: 06/10/98
date of end of treatment; 03/11/98
date of necropsy
: 04/11/98
No findings noted
'
Inflammatory cell foci (minimal)
Tubular atrophy, cortical (minimal)
Tubular atrophy, cortical (minimal)
Hyaline cast(s) (minimal)
Extracapsular nodule present
Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (minimal)
Lymphoid atrophy - involution (minimal) Lynphocytolysis, increased (slight)
No abnormalities found in: Pancreas, Testes/l, Testes/2, Adrenal Gland/2, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2.
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Test Article: telomer alcohol/telomer acrylate/ /pfhs/pfos
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. s
242933
Propath no. :
99010
Date
: 21.Apr.1999
Individual Animal Data Records
Animal No: SEX: DOSE GROUP:
57 Male
MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS
Animal No:
58
MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS
Animal No:
59
MACROSCOPIC FINDINGS
.
days of treatment :29
sacrifice group
:recovery group
necropsy status
:planned recovery
date of start of treatment: 06/10/98
date of end of treatment: 03/11/98
date of necropsy
:18/11/98
No findings noted
No tissues examined
days of treatment :29
sacrifice group
:recovery group
necropsy status
:planned recovery
date of start of treatment: 06/10/98
date of end of treatment: 03/11/98
date of necropsy
:18/11/98
No findings noted
No tissues examined
days of treatment : 29
sacrifice group
: recovery group
necropsy status
: planned recovery
date of start of treatment: 06/10/98
date of end of treatment: 03/11/98
date of necropsy
: 18/11/98
No findings noted
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Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ ,'PFHS/PFOS
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
.
NOTOX no. Prbpath no. Date
242933 99010
21.Apr.1999
Individual Animal Data Records
Animal No:
SEX: DOSE GROUP:
59 Male
MICROSCOPIC FINDINGS
Animal No:
61
MACROSCOPIC FINDINGS
Eyes
MICROSCOPIC FINDINGS Eyes
Animal No:
62
MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS
. No tissues examined
days of treatment : 29
sacrifice group
: recovery group 2
necropsy status
: planned recovery 2
date of start of treatment: 06/10/98
date of end of treatment: 03/11/98
date of necropsy
: 02/12/98
lens: right side Reduced in size
No abnormalities were found in any of the other tissues examined
Periorbital inflammation due to blood sampling (moderate) (correlates to GROSS finding)
days of treatment sacrifice group
: 29 : recovery group 2
necropsy status
:planned recovery 2
date of start of treatment: 06/10/98
date of end of treatment: 03/11/98
date of necropsy
: 02/12/98
No findings noted
No tissues examined
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Page 104/146
Test Article: telomer alcohol/ telomer acrylate/
/ pfhs/ pfos
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. Propath no. Date
242933 99010
21.A p r . 1999
Individual Animal Data Records
Animal No: SEX: DOSE GROUP:
63 Male
MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS
Animal No: SEX:
145 Female
MACROSCOPIC FINDINGS
M ICRO SCO PIC FINDINGS
Liver Spleen
Mandibular Lymph Node/ 1
.
days of treatment : 29
sacrifice group
: recovery group 2
necropsy status
:planned recovery 2
date of start of treatment: 06/10/98
date of end of treatment: 03/11/98
date of necropsy
: 02/12/98
No findings noted
No tissues examined
days of treatment : 28
sacrifice group
: terminal sacrifice
necropsy status
: planned terminal
date of start of treatment: 08/10/98
date of end of treatment: 04/11/98
date of necropsy
: 05/11/98
No findings noted
Inflammatory cell foci (minimal)
Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (slight)
Lymphoid hyperplasia (slight)
No abnormalities found in: Pancreas, Kidney/1, Kidney/2, Ovary/1, Ovary/2, Adrenal Gland/1, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/2.
BACK TO MAIN
PATHOLOGY REPORT
Test Article: telomer alcohol/ telomer acrylate/
./pf h s/ pfo s
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
Page 105/146
NOTOX no. Propath no. Date
242933 99010
21.A p r . 1999
Individual Animal Data Records
Animal No: SEX:
DOSE GROUP:
146 Female
M ACROSCOPIC FIND IN GS
MICROSCOPIC FINDINGS Liver
Spleen
Animal No:
147
MACROSCOPIC FINDINGS
.
days of treatment :28
sacrifice group
:terminal sacrifice
necropsy status
:planned terminal
date of start of treatment: 08/10/98
date of end of treatment: 04/11/98
date of necropsy
: 05/11/98
.
No findings noted
'
Inflammatory cell foci (slight) Single cell necrosis (minimal)
Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (slight)
No abnormalities found in: Pancreas, Kidney/1, Kidney/2, Ovary/1, Ovary/2, Adrenal G l and/1, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2.
days of treatment : 28
sacrifice group
: terminati sacrifice
necropsy status
: planned terminal
date of start of treatment: 08/10/98
date of end of treatment: 04/11/98
date of necropsy
: 05/11/98
No findings noted
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T e s t A r t i c l e : TELOMER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PFOS
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. Propath no. Date
242933 9910
2 1 .Apr.1999
Individual Animal Data Records
Animal No:
SEX: DOSE GROUP:
147
F em a le
M ICRO SCO PIC FINDINGS
Liver
Kidney/1
Spleen
Mandibular Lymph Node/ 1
Animal No:
148
M ACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS Liver Kidney/1 Spleen
' Thymus
-
Inflammatory cell foci (minimal) Single cell necrosis (minimal) Cystic tubules (slight) Hemosiderin pigment (slight) Lymphoid hyperplasia (slight)
No abnormalities found in: Pancreas, Kidney/2, Ovary/1, Ovary/2, Adrenal Gland/1, Adrenal Gl and/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/2.
days of treatment : 28
sacrifice group
: terminal sacrifice
necropsy status
:planned terminal
date of start of treatment: 08/10/98
date of end of treatment: 04/11/98
date of necropsy
: 05/11/98
No findings noted
Inflammatory cell foci (slight)
Interstitial inflammation, lymphocytic (minimal)
Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (slight)
Lymphocytolysis, increased (minimal)
No abnormalities found in: Pancreas, Kidney/2, Ovary/1, Ovary/2, Adrenal Gland/1, Adrenal Gl a n d / 2, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2.
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Te s t Article: telomer alcohol/ telqmer acrylate/
/ pfhs/ pfos
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. Propath no. Date
242933 99010
2 1 .Apr.1999
Individual Animal Data Records
Animal No: SEX:
DOSE GROUP:
149 Female
MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS Liver Spleen
Animal No:
150
MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS Liver
Spleen Mandibular Lymph Node/
1
days of treatment :29
sacrifice group
:terminal sacrifice
necropsy status
:planned terminal
date of start of treatment: 08/10/98
date of end of treatment: 05/11/98
date of necropsy
:06/11/98
No findings noted
Inflammatory cell foci (slight)
Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (slight)
No abnormalities found in: Pancreas, Kidney/1, Kidney/2, Ovary/1, Ovary/2, Adrenal Gland/1, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2.
days of treatment : 29
sacrifice group
: terminal sacrifice
necropsy status
:planned terminal
date of start of treatment: 08/10/98
date of end of treatment: 05/11/98
date of necropsy
: 06/11/98
No findings noted
Inflammatory cell foci (slight) Hematopoietic cell foci (minimal)
Hemosiderin pigment (moderate)
Plasmacytosis (slight) Lymphoid hyperplasia (slight)
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Te s t Article: telomer alcohol/ telomer acr ylate/
/ pfhs/ pfos
T e s t S y s t e m : E x p l o r a t o r y 2 8 - D a y G a v a g e S t u d y i n Rats'
Sponsor
: 3M Toxicology Services
N O TOX no. Propath no. Date
242933 99010
21.A p r . 1999
Individual Animal Data Records
Animal No: SEX: DOSE GROUP:
150 Female
MICROSCOPIC FINDINGS (continued)
Animal No:
151
MACROSCOPIC FINDINGS
Uterus
MICROSCOPIC FINDINGS liver Uterus Spleen
.
No abnormalities found in: Pancreas, Kidney/1, KLdney/2, Ovary/1, Ovary/2, Adrenal Gland/1, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/2.
days of treatment : 29
sacrifice group
: terminal sacrifice
necropsy status
:planned terminal .
date of start of treatment: 08/10/98
date of end of treatment: 05/11/98
date of necropsy
: 06/11/98
left side Cyst(s)
No abnormalities were found in any of the other tissues examined
Inflammatory cell foci (slight)
Cyst(s) present simple serosal) (correlates to GROSS finding)
Hemosiderin pigment (slight)
No abnormalities found in: Pancreas, Kidney/1, Kidney/2, Ovary/1, Ovary/2, Adrenal Gland/1, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2.
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Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PFOS
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. Propath no. Date
242933 99010
21.Apr.1999
Individual Animal Data Records
Animal Ho: SEX: DOSE GROUP:
152 Female
MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS Liver
Kidney/1 Spleen
Thymus
Animal No:
153
M ACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS
.
days of treatment : 29
sacrifice group
: terminal sacrifice
necropsy status
:planned terminal
date of start of treatment: 08/10/98
date of end of treatment: 05/11/98
date of necropsy
: 06/11/98
No findings noted
Inflammatory cell foci (minimal) Single cell necrosis (minimal)
Tubular atrophy, cortical (minimal)
Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (slight)
Lymphoid atrophy - involution (minimal)
No abnormalities found in: Pancreas, Kidney/2, Ovary/1, Ovary/2, Adrenal Gland/1, Adrenal Gland/2, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2.
days of treatment :29
sacrifice group
:recovery group
necropsy status
:planned recovery
date of start of treatment: 08/10/98
date of end of treatment: 05/11/98
date of necropsy
:20/11/98
No findings noted
No tissues examined
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Page 110/146
Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ -/PFHS/PFOS
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. Propath no'. Date
242933 99010
21.Apr.1999
Individual Animal Data Reeoxds
Animal No: SEX: DOSE GROUP:
154 Female
MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS
Animal No:
155
MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS
Animal No:
157
MACROSCOPIC FINDINGS
days of treatment : 29
sacrifice group
: recovery group
necropsy status
: planned recovery
date of start of treatment: 08/10/98
date of end of treatment: 05/11/98
date of necropsy
: 20/11/98
No findings noted
No tissues examined
days of treatment :29
sacrifice group
:recovery group
necropsy status
:planned recovery
date of start of treatment: 08/10/98.
date of end of treatment: 05/11/98
date of necropsy
:20/11/98
No findings noted
No tissues examined
days of treatment : 29
sacrifice group
: recovery group 2
necropsy status
:planned recovery 2
date of start of treatment: 08/10/98
date of end of treatment: 05/11/98
date of necropsy
: 04/12/98
No findings noted
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Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PFOS
Test System : Exploratory'28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NQTOX no. Propath no. Date
242933 99010
21.Apr.1999
Individual Animal Data Records
Animal No: SEX:
DOSE GROUP:
157 Female
MICROSCOPIC FINDINGS
Animal No:
158
MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS
Animal No:
159
MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS
No tissues examined
days of treatment : 29
sacrifice group
: recovery group 2
necropsy status
:planned recovery 2
date of start of treatment: 08/10/98
date of end of treatment: 05/11/98
date of necropsy
: 04/12/98
No findings noted
No tissues examined
d ays o f treatm en t
: 29
s a c r if ic e group
: re co v e ry group 2
n ecropsy sta tu s
:p la n n e d r e c o v e r y
d a t e o f s t a r t o f t r e a t m e n t : 08/10/98
d a te o f end o f trea tm en t: 05/11/98
d ate o f n ecrop sy
: 04/12/98
2
No findings noted
No tissues examined
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Page 112/146
Teat Article: telomer alcohol/telomer acrylate/ /pfhs/pfos
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. Propath no. Date
242933 99010
21.A p r . 1999
Individual Animal Data Records
Animal No: SEX: DOSE GROUP:
65 Hale PFHS
MACROSCOPIC FINDINGS
M ICRO SCO PIC FINDINGS L iv e r
Kidney/1 Spleen
Animal No:
66
M ACROSCOPIC FIN D IN GS
Lungs Stomach
Testes
days of treatment : 28
sacrifice group
: terminal sacrifice
necropsy status
:planned terminal
date of start of treatment: 06/10/98
date of end of treatment: 02/11/98
date of necropsy : 03/11/98
No findings noted
In fla m m a to ry c e l l f o c i ( s lig h t ) S in g le c e l l n e c r o s is (m in im al)
Midzonal/centrilobular hypertrophy diffuse (moderate)
Hepatocellular vacuolation (slight)
Tubular atrophy, cortical (minimal)
Hemopoiesis, primarily erythropoiesis (slight)
No abnormalities found in: Pancreas, Kidney/2, Testes/l, Testes/2, Adrenal Gland/1, Adrenal Gland/ 2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2.
days of treatment : 28
sacrifice group
: terminal sacrifice
necropsy status
:planned terminal
date of start of treatment: 06/10/98
date of end of treatment: 02/11/98
date of necropsy : 03/11/98
Hemorrhage
glandular mucosa Hemorrhage
Reduced in size
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Test Article; telomer alcohol/telomer acrylate/ /pfhs/pfos
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
; 3M Toxicology Services
NQTOX no. Propath no. Date
242933 99010
21.Apr.1999
Individual Animal Data Records
Animal No: SEX: DOSE GROUP:
66
Male
PFHS
MACROSCOPIC FINDINGS (continued)
Epididymides
M ICROSCOPIC FINDINGS
Lungs
Stomach Liver
Pancreas Kidney/1 Testes/1
Testes/2
Epididymides
Spleen
Mandibular Lymph Node/ 1
Mandibular Lymph Node/ 2
Reduced in size
-
No abnormalities were found in any of the other tissues examined
Intra-alveolar blood, necropsy artefact present (correlates to GROSS finding)
Vascular mineralisation, focal (minimal)
No micro correlate
Inflammatory cell foci (minimal) Midzonal/centrilobular hypertrophy, diffuse
(moderate)
Apoptosis/single cell necrosis, exocrine (minimal)
Tubular atrophy, cortical (minimal)
Seminiferous tubular atrophy (severe) (correlates to GROSS finding)
Seminiferous tubular atrophy (severe) (correlates to
GROSS f in d in g )
Atrophy (moderate) (correlates to GROSS finding) Aspermia present
Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (minimal)
Plasmacytosis (moderate)
Congestion/erythrophagocytosis (minimal)
No abnormalities found in: Kidney/2, Adrenal Gland/ 1, Adrenal Gland/2, Thymus, Mesenteric Lymph Node.
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Page 114/146
Test Article: telomer alcohol/telomer acrylate/ /pfhs/pfos
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NQTOX no.. :
242933
Propath no. :
99010
Date
: 21.Apr.1999
Individual Animal Data Records
Animal No: SEX: COSE GROUP:
67 Hale PFHS
MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS liver
KLdney/1 Spleen Mandibular lymph Node/
1
Animal No:
68
MACROSCOPIC FINDINGS
.
days of treatment :28
sacrifice group
:terminal sacrifice
necropsy status
:planned terminal
date of start of treatment: 06/10/98
date of end of treatment: 02/11/98
date of necropsy :03/11/98
No findings noted
Inflammatory cell foci (slight)
Single cell necrosis (minimal)
Midzonal/centrilobular hypertrophy, diffuse
(moderate)
-
Hepatocellular vacuolation (minimal)
Coagulative necrosis, focal (minimal)
Tubular atrophy, cortical (minimal)
Hemopoiesis, primarily erythropoiesis (slight)
Plasmacytosis (slight)
No abnormalities found in: Pancreas, KLdney/2,
Testes/l, Testes/2, Adrenal Gland/1, Adrenal Gland/ 2, Thymus, Mesenteric nymph Node, Mandibular Lymph
Node/2.
days of treatment :28
sacrifice group
:terminal sacrifice
necropsy status
:planned terminal
date of start of treatment: 06/10/98
date of end of treatment: 02/11/98
date of necropsy :03/11/98
No findings noted
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Page 115/146
Test Article: telomer alcohol/telomer acrylate/ . ./pfhs/peos
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NO.TOX no. Propath no. Date
242933 99010
21.Apr.1999
Individual Animal Data Records
Animal No: SEX: DOSE GROUP:
68 Male PFHS
MICROSCOPIC FINDINGS
Liver
Pancreas Kidney/1
Spleen
Animal No:
69
MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS Liver
KLdney/1 Adrenal Gland/1 Spleen
Inflammatory cell foci (slight) Midzonal/centrilobular hypertrophy, diffuse
(moderate)
Apoptosis/slngle cell necrosis, exocrine (minimal)
Tubular atrophy, cortical (minimal) Interstitial inflammation, lymphocytic (minimal)
Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (slight)
No abnormalities found in: KLdney/2, Testes/l, Testes/2, Adrenal Gland/1, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2.
days of treatment :29
sacrifice group
:terminal sacrifice
necropsy status
:planned terminal
date of start of treatment: 06/10/98
date of end of treatment: 03/11/98
date of necropsy :04/11/98
No findings noted
Inflammatory cell foci (slight) Single cell necrosis (minimal) Midzonal/centrilobular hypertrophy, diffuse
(moderate)
Interstitial inflammation, lymphocytic (minimal)
Extracapsular nodule present
Hemopoiesis, primarily erythropoiesis (minimal)
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Test Article: telomer alcohol/telcmer acrylate/ ,'pfhs/pfos
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. Propath no. Date
242933
99010 21.A p r . 1999
Individual Animal Data Records
Animal No: SEX: DOSE GROUP:
69 Male PFHS
MICROSCOPIC FINDINGS (continued)
Animal No: 70 MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS Liver
Pancreas Kidney/1 Spleen
.
No abnormalities found in: Pancreas, Kidney/2, Testes/l, Testes/2, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2.
days of treatment : 29
sacrifice group
: terminal sacrifice
necropsy status
: planned terminal
date of start of treatment: 06/10/98
date of end of treatment: 03/11/98
date of necropsy : 04/11/98
No findings noted
Inflammatory cell foci (slight) Single cell necrosis (minimal) Midzonal/centrilobular hypertrophy, diffuse (slight)
Inflammation, mixed granulocytic/lymphocytic (minimal)
Interstitial inflammation, lymphocytic (minimal)
Hemopoiesis, primarily erythropoiesis (slight)
No abnormalities found in: Kidney/2, Testes/l, Testes/2, Adrenal Gland/1, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2.
BACK TO MAIN
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Test Article: telomer alcohol/telomer acrylate/ ,pfhs/pfos
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
Page 117/146
NOTOX no. Propath n o . Date
242933 99010
21.Apr.1999
Individual Animal Data Records
Animal No: SEX: DOSE GROUP:
71 Male PFHS
MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS Liver
KLdney/1 Spleen
Mandibular Lymph Node/ 1
Mandibular Lymph Node/ 2
Animal No:
72
MACROSCOPIC FINDINGS
Liver
.
days of treatment :29
sacrifice group
:terminal sacrifice
necropsy status
:planned terminal
date of start of treatment: 06/10/98
date of end of treatment: 03/11/98
date of necropsy :04/11/98
No findings noted
Inflammatory cell foci (slight) Single cell necrosis (minimal) Midzonal/centrilobular hypertrophy, diffuse (slight)
Cystic tubules (slight)
Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (minimal)
Plasmacytosis (slight)
Plasmacytosis (slight)
No abnormalities found in: Pancreas, Kidney/2, Testes/1, Testes/2, Adrenal Gland/1, Adrenal Gland/ 2, Thymus, Mesenteric Lymph Node.
days of treatment : 29
sacrifice group
: terminal sacrifice
necropsy status
:planned terminal
date of start of treatment: 06/10/98
date of end of treatment: 03/11/98
date of necropsy : 04/11/98
Enlarged
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Test Article: telomer alcohol/telomer acrylate/ V pehs/pfos
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. Propath no. Date
242933 99010
21.Apr.1999
Individual Animal Data Records
Animal No: SEX: DOSE GROUP:
72 Hale PFHS
MACROSCOPIC FINDINGS (continued)
MICROSCOPIC FINDINGS Liver
Spleen
Mandibular Lymph Node/ 1
Mandibular Lymph Node/ 2
Animal No:
73
MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS Liver
No abnormalities were found in any of the other tissues examined
Inflammatory cell foci (slight) Single cell necrosis (minimal) Midzonal/centrilobular hypertrophy, diffuse
(moderate) (correlates to GROSS finding) Hepatocellular vacuolation (minimal)
Hemopoiesis, primarily erythropoiesis (slight) Hemosiderin pigment (slight)
Plasmacytosis (slight) Congestion/erythrophagocytosis (slight)
Congestion/erythrophagocytosis (minimal)
No abnormalities found in: Pancreas, Kidney/1, Kidney/2, Testes/l, Testes/2, Adrenal Gland/1, Adrenal Gland/2, Thymus, Mesenteric Lymph Node.
days o f treatm en t
: 29
sacrifice group
:recovery group
necropsy status
:planned recovery
date of start of treatment: 06/10/98
date of end of treatment: 03/11/98
date of necropsy :18/11/98
No findings noted
Inflammatory cell foci (moderate)
Single cell necrosis (slight) Midzonal/centrilobular hypertrophy, diffuse (slight)
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Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PPOS
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NQTOX no. Propath no. Date
242933 '99010 21.Apr.1999
Individual Animal Data Records
Animal No: SEX: DOSE GROUP:
74 Male PFHS
MACROSCOPIC FINDINGS
Testes
MICROSCOPIC FINDINGS Liver
Testes/l
Testes/2
Animal No:
75
MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS Liver
days of treatment :29
sacrifice group
:recovery group
necropsy status
:planned recovery
date of start of treatment: 06/10/98
date of end of treatment: 03/11/98
date of necropsy :18/11/98
Reduced in size
No abnormalities were found in any of the other tissues examined
Inflammatory cell foci (slight) Single cell necrosis (minimal)
Seminiferous tubular atrophy (moderate) (correlates to GROSS finding)
Seminiferous tubular atrophy (moderate) (correlates to GROSS finding)
days of treatment : 29
sacrifice group
: recovery group
necropsy status
:planned recovery
date of start of treatment: 06/10/98
date of end of treatment: 03/11/98
date of necropsy : 18/11/98
No findings noted
Inflammatory cell foci (slight) Single cell necrosis (minimal) Midzonal/centrilobular hypertrophy, diffuse
(minimal)
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Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ ,/PFHS/PFOS
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NO.TOX no. :
242933
Propath no. :
99010
Date
: 21.Apr.1999
Individual Animal Data Records
Animal No: SEX:
DOSE GROUP:
77 Hale
PFHS
M ACROSCOPIC FIND IN GS
MICROSCOPIC FINDINGS Liver
Animal No:
78
MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS Id.ver
Animal No:
79
MACROSCOPIC FINDINGS
days of treatment : 29
sacrifice group
: recovery group 2
necropsy status
:planned recovery 2
date of start of treatment: 06/10/98
date of end of treatment: 03/11/98
date of necropsy : 02/12/98
No findings noted
Inflammatory cell foci (slight) Single cell necrosis (minimal)
days of treatment : 29
sacrifice group : recovery group 2
necropsy status
: piaimed recovery 2
date of start of treatment: 06/10/98
date of end of treatment: 03/11/98
' ' date of necropsy : 02/12/98
No findings noted
Inflammatory cell foci (slight) Single cell necrosis (minimal)
days of treatment : 29
sacrifice group
: recovery group 2
necropsy status
:planned recovery 2
date of start of treatment: 06/10/98
date of end of treatment: 03/11/98
date of necropsy : 02/12/98
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Test Article: telomer alcohol/telomer acrylate/ /pfhs/pfos
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX n o . P ro p ath n o . D ate
242933 99010
21.Apr.1999
Individual Animal Data Records
Animal No: SEX: DOSE GROUP:
79 Male PFHS
MACROSCOPIC FINDINGS (continued)
MICROSCOPIC FINDINGS liver
Animal No: SEX:
161 Female
MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS liver Kidney/1 Spleen
. .
No findings noted
Inflammatory cell foci (slight) Hepatocellular vacuolation (minimal)
days of treatment : 28
sacrifice group
: terminal sacrifice
necropsy status
:planned terminal
date of start of treatment: 08/10/98
date of end of treatment: 04/11/98
date of necropsy : 05/11/98
No findings noted
"
Inflammatory cell foci (slight)
Tubular epithelial hypertrophy, focal (minimal)
Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (slight)
No abnormalities found in: Pancreas, Kidney/2, Ovary/1, Ovary/2, Adrenal Gland/1, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2.
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Test Article: tblomer alcohol/telomer acrylate/ /pfhs/pfos
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. Propath no. Date
242933 99010
21.Apr.1999
Individual Animal Data Records
Animal Mo: SEX: DOSE GROUP:
162 Female PFHS
MACROSCOPIC FINDINGS
.
MICROSCOPIC FINDINGS Liver
Spleen
Animal No:
163
MACROSCOPIC FINDINGS
days of treatment : 28 sacrifice group : terminal sacrifice necropsy status : planned terminal date of start of treatment: 08/10/98 date of end of treatment: 04/11/98 date of necropsy : 05/11/98
No findings noted
Inflammatory cell foci (slight) Single cell necrosis (minimal)
Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (slight)
No abnormalities found in: Pancreas, Kidney/1, Kidney/2, Ovary/1, Ovary/2, Adrenal Gland/1, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2.
days of treatment : 28 ' sacrifice group : terminal sacrifice
necropsy status
: planned terminal
d a te o f s t a r t o f tre a tm e n t: 08/10/98
date of end of treatment: 04/11/98
date of necropsy : 05/11/98
No findings noted
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Page 123/14S
Test Article: telomer alcohol/telomer acrylate/ /pfhs/pfos
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOJOX no. Propath no. Date
242933 99010
21.Apr.1999
Individual Animal Data Records
Animal No: SEX: DOSE GROUP:
163 Female PFHS
MICROSCOPIC FINDINGS
Liver
Spleen
Thymus
Mandibular Lymph Node/ 1
Animal No:
164
MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS Liver Spleen
Thymus
Inflammatory cell foci (moderate) Single cell necrosis (minimal)
Hemopoiesis, primarily erythropoiesis (slight) Hemosiderin pigment (slight)
Lynphocytolysis, increased (minimal)
Plasmacytosis (slight)
No abnormalities found in: Pancreas, Kidney/1, Kidney/2, Ovary/1, Ovary/2, Adrenal Gland/1, Adrenal Gland/2, Mesenteric Lymph Node, Mandibular Lymph Node/2.
days of treatment :28
sacrifice group
:terminal sacrifice
necropsy status
:planned terminal
date of start of treatment: 08/10/98
date of end of treatment: 04/11/98
date of necropsy :05/11/98
No findings noted
Inflammatory cell foci (slight)
Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (slight)
Lymphoid atrophy - involution (slight)
No abnormalities found in: Pancreas, Kidney/1, Kidney/2, Ovary/1, Ovary/2, Adrenal Gland/1, Adrenal Gland/2, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2.
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Page 124/146
Test Article: telomer alcohol/telomer acrylate/ ,/pfhs/pfos
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NQTOX no. Propath no. Date
242933 99010
21.Apr.1999
Individual Animal Data Records
Animal No: SEX: DOSE GROUP:
165 Female PFHS
MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS Liver Pancreas Spleen
Animal No: 166 MACROSCOPIC FINDINGS
days of treatment :29
sacrifice group
:tennined, sacrifice
necropsy status
:planned terminal
date of start of treatment: 08/10/98
date of end of treatment: 05/11/98
date of necropsy :06/11/98
No findings noted
Inflammatory cell foci (minimal) Single cell necrosis (minimal)
Apoptosis/single cell necrosis, exocrine (minimal)
Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (slight)
No abnormalities found in: KLdney/1, Kidney/2, Ovary/1, Ovary/2, Adrenal Gland/1, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2.
days of treatment : 29
sacrifice group
: terminal sacrifice
necropsy status
:planned terminal
date of start of treatment: 08/10/98
date of end of treatment: 05/11/98
date of necropsy : 06/11/98
No findings noted
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Page 125/146
Test Article: telomer alcohol/telomer acrylate/ 'pfhs/pfos
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. Propath no. Date
242933 99010
21.Apr.1999
Individual Animal Data Records
Animal No: SEX: DOSE GROUP:
166 Female PFHS
MICROSCOPIC FINDINGS
Liver
Adrenal Gland/1
Spleen
MandibularLymph Node/ 1
Animal No:
167
MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS Liver
Spleen
Inflammatory cell foci (minimal) Extracapsular nodule present Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (slight) Lymphoid hyperplasia (slight)
No abnormalities found in: Pancreas, Kidney/1, Kidney/2, Ovary/1, Ovary/2, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/2.
days of treatment : 29
sacrifice group : terminal sacrifice
necropsy status
: planned terminal
date of start of treatment: 08/10/98
date of end of treatment: 05/11/98
date of necropsy : 06/11/98
No findings noted
Inflammatory cell foci (minimal) Single cell necrosis (minimal)
Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (slight)
No abnormalities found in: Pancreas, Kidney/1, Kidney/2, Ovary/1, Ovary/2, Adrenal Gland/1, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular-Lymph Node/2.
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Page 126/146
Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PFOS
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NQTOX no. :
242933
Propath no. :
99010
Date
: 21.Apr.1999
Individual Animal Data Records
Animal No: SEX: DOSE GROUP:
168 Female PFHS
MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS Liver
Kidney/1 Spleen
Animal No:
169
MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS
days of treatment : 29
sacrifice group
: terminalsacrifice
necropsy status
:planned terminal
date of start of treatment: 08/10/98
date of end of treatment: 05/11/98
date of necropsy : 06/11/98
.
No findings noted
Inflammatory cell foci (minimal) Single cell necrosis (minimal) Hepatocellular vacuolation (minimal)
Cystic tubules (minimal)
Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (moderate)
No abnormalities found in: Pancreas, Kidney/2, Ovary/1, Ovary/2, Adrenal Gland/1, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2.
'
days of treatment : 29 sacrifice group : recovery group necropsy status : planned recovery date of start of treatment: 08/10/98 date of end of treatment: 05/11/98 date of necropsy : 20/11/98
No findings noted
No tissues examined
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Page 127/146
Test Article: telomer alcohol/telomer acrylate/ /pfhs/pfos
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. Propath no. Date
242933 99010
21.Apr.1999
Individual Animal Data Records
Animal No: SEX: DOSE GROUP:
170 Female PFHS
MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS
Animal No:
171
MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS
Animal No:
173
MACROSCOPIC FINDINGS
days of treatment : 29
sacrifice group
: recovery group
necropsy status
: planned recovery
date of start of treatment: 08/10/98
date of end of treatment: 05/11/98
date of necropsy : 20/11/98
No findings noted
No tissues examined
days of treatment : 29
sacrifice group
: recovery group
necropsy status
: planned recovery
date of start of treatment: 08/10/98
date of end of treatment: 05/11/98
date of necropsy : 20/11/98
No findings noted
No tissues examined
days of treatment : 29
sacrifice group
: recovery group 2
necropsy status
:planned recovery 2
date of start of treatment: 08/10/98
date of end of treatment: 05/11/98
date of necropsy : 04/12/98
No findings noted
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Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ ,/PFHS/PFOS
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. Propath no. Date
242933 99010
21.Apr.1999
Individual Animal Data Records
Animal No: SEX:
DOSE GROUP:
173 Female
PFHS
MICROSCOPIC FINDINGS
Animal No: 174 MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS
Animal No: 175 MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS
No tissues examined
days of treatment : 29
sacrifice group
: recovery group 2
necropsy status
: planned recovery 2
date of start of treatment: 08/10/98
date of end of treatment: 05/11/98
date of necropsy : 04/12/98
No findings noted
No tissues examined
days of treatment : 29
sacrifice group
: recovery group 2
necropsy status
: planned recovery 2
date of start of treatment: 08/10/98
date of end of treatment: 05/11/98
date of necropsy : 04/12/98
No findings noted
No tissues examined
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Page 129/146
Teat Article: telomer alcohol/telomer acrylate/ /pfhs/pfos
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NQTOX no. Propath no. Date
242933 99010
21.A p r . 1999
Individual Animal Data Records
Animal No:
SEX:
DOSE GROUP:
81
H ale
PFOS.
MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS Liver
Kidney/1 Spleen
Mandibular Lymph Node/ 1
Animal No:
82
MACROSCOPIC FINDINGS
.
days of treatment :28
sacrifice group
:terminal sacrifice
necropsy status
:planned terminal
date of start of treatment: 06/10/98
date of end of treatment: 02/11/98
date of necropsy :03/11/98
No findings noted
.
Inflammatory cell foci (slight) Single cell necrosis (minimal) Midzonal/centrilobular hypertrophy, diffuse (slight) Hematopoietic cell foci (minimal)
Tubular atrophy, cortical (minimal)
Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (minimal)
Plasmacytosis (moderate)
No abnormalities found in: Pancreas, Kidney/2, Testes/l, Testes/2, Adrenal Gland/1, Adrenal Gland/
2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/2.
days of treatment : 28
sacrifice group
: terminal sacrifice
necropsy status
:planned terminal
date of start of treatment: 06/10/98
date of end of treatment: 02/11/98
date of necropsy : 03/11/98
No findings noted
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Page 130/146
Test Article: telomer alcohol/telomer acrylate/ /pfhs/pfos
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NQTOX no. Propath no. Date
242933 99010
21.Apr.1999
Individual Animal Data Records
Animal Ko: 82
SEX:
Male
DOSE GROUP : PFOS
MICROSCOPIC FINDINGS
Liver
Pancreas Kidney/1 Spleen
Animal No:
83
MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS Liver
Kidney/1 Kidney/2
Inflammatory cell foci (slight) Midzonal/centrilobular hypertrophy, diffuse
(moderate) Hepatocellular vacuolation (minimal)
Apoptosis/single cell necrosis, exocrine (slight)
Tubular atrophy, cortical (minimal)
Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (minimal)
No abnormalities found in: Kidney/2, Testes/1, Testes/2, Adrenal Gland/1, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2.
days of treatment : 28
sacrifice group
: terminal sacrifice
necropsy status
:planned terminal
date of start of treatment: 06/10/98
date of end of treatment: 02/11/98
date of necropsy : 03/11/98
No findings noted
Inflammatory cell foci (slight) Single cell necrosis (minimal) Midzonal/centrilobular hypertrophy, diffuse
(moderate) Hepatocellular vacuolation (minimal)
Interstitial inflammation, lymphocytic (slight) Cystic tubules (slight)
Interstitial inflammation, lymphocytic (minimal)
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Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ "VPFHS/PFOS
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. Propath no. Date
242933 99010
21.Apr.1999
Individual Animal Data Records
Animal No: SEX: DOSE GROUP:
83 Hale PFOS
MICROSCOPIC FINDINGS (continued)
Testes/l
Spleen
Animal No: 84 MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS Liver
Kidney/1
Adrenal Gland/1 Adrenal Gland/2 Spleen Mandibular Lymph Node/
1
Spermatidic giant cell(s) (minimal)
Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (minimal)
No abnormalities found in: Pancreas, Testes/2, Adrenal Gland/1, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2.
days of treatment :28
sacrifice group
:terminal sacrifice
necropsy status
:planned terminal
date of start of treatment: 06/10/98
date of end of treatment: 02/11/98
date of necropsy :03/11/98
No findings noted
Inflammatory cell foci (slight) Single cell necrosis (minimal) Midzonal/centrilobular hypertrophy, diffuse
(moderate)
Tubular atrophy, cortical (minimal) Interstitial inflammation, lymphocytic (minimal)
Atrophy, cortical diffuse (slight)
Atrophy, cortical diffuse (slight)
Hemopoiesis, primarily erythropoiesis (minimal)
Plasmacytosis (slight)
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Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PFOS
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
N o y o x no. Propath no. Date
242933 99010
21.A p r . 1999
Individual animal Data Records
animal No: SEX: DOSE GROUP:
84 Male PFOS
MICROSCOPIC FINDINGS (continued)
animal No: 85 MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS Liver
Kidney/1 Spleen
Animal No:
86
MACROSCOPIC FINDINGS
.
No abnormalities found in: Pancreas, Kidney/2, Testes/1, Testes/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/2.
days of treatment :29
sacrifice group
:terminal sacrifice
necropsy status
:planned terminal
date of start of treatment: 06/10/98
date of end of treatment: 03/11/98
date of necropsy :04/11/98
No findings noted
Inflammatory cell foci (minimal)
.
Midzonal/centrilobular hypertrophy, diffuse
(moderate)
Tubular atrophy, cortical (minimal)
Hemopoiesis, primarily erythropoiesis (minimal)
No abnormalities found in: Pancreas, Kidney/2, Testes/l, Testes/2, Adrenal Gland/1, Adrenal Gland/ 2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2.
days of treatment : 29
sacrifice group
: terminal sacrifice
necropsy status
: planned terminal
date of start of treatment: 06/10/98
date of end of treatment: 03/11/98
date of necropsy : 04/11/98
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Test Article: telomer alcohol/telomer acrylate/ /pfhs/pfos
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOXOX no. Propath no. Date
242933 99010
21.Apr.1999
Individual Animal Data Records
Animal No: SEX: DOSE GROUP:
86 Male PFOS
MACROSCOPIC FINDINGS (continued)
MICROSCOPIC FINDINGS Liver
Kidney/1
Kidney/2 Adrenal Gland/1 Adrenal Gland/2 Spleen Thymus
Animal No:- 87 MACROSCOPIC FINDINGS
.
No findings noted
'
Inflammatory cell foci (slight) Single cell necrosis (minimal) Midzonal/centrilobular hypertrophy, diffuse (slight) Hepatocellular vacuolation (minimal)
Tubular atrophy, cortical (minimal) Hyaline cast(s) (minimal)
Tubular atrophy, cortical (minimal)
Vacuolation, multifocal in z. fasiculata (minimal)
Vacuolation, multifocal in z. fasiculata (minimal)
Hemopoiesis, primarily erythropoiesis (slight)
Lymphoid atrophy - involution (slight)
No abnormalities found in: Pancreas, Testes/l, Testes/2, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2.
days of treatment : 29
sacrifice group
: terminal sacrifice
necropsy status
:planned terminal
date of start of treatment: 06/10/98
date of end of treatment: 03/11/98
date of necropsy : 04/11/98
No findings noted
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Teat Article: telomer alcohol/telomer acrylate/ /pfhs/pfos
Teat System : Exploratory 28-Day Gavage Study in Rata
Sponaor
: 3M Toxicology Services
NOTOX no. Propath no. Date
242933 99010
21.A p r . 1999
Individual Animal Data Records
Animal No: SEX:
DOSE GROUP:
87 Male
PFOS
MICROSCOPIC FINDINGS
Liver
Kdney/1 Spleen
Animal No:
88
MACROSCOPIC FINDINGS
M ICROSCOPIC FINDINGS L iv e r
Pancreas Adrenal Gland/1 Adrenal Gland/2
Inflammatory cell foci (alight)
S in g le c e l l n e c r o s is (m in im al) M id z o n a l/ c e n trilo b u la r h yp ertro p h y ,
(m od erate)
Hepatocellular vacuolation (slight)
d iffu s e
T u b u la r a tr o p h y , c o r t i c a l (m in im al) H y a lin e c a s t ( s ) (m in im al)
H e m o p o ie sis, p r im a r ily e r y t h r o p o ie s ia (m in im al)
No abnormalities found in: Pancreas, Kidney/2, Testea/1, Testes/2, Adrenal Gland/1, Adrenal Gland/ 2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2.
days of treatment : 29
sacrifice group
: terminal sacrifice
necropsy status
:planned terminal
date of start of treatment: 06/10/98
date of end of treatment: 03/11/98
date of necropsy : 04/11/98
No findings noted
Inflammatory cell foci (slight) Single cell necrosis (minimal) Midzonal/centrilobular hypertrophy, diffuse
(moderate)
Apoptosis/single cell necrosis, exocrine (minimal)
Vacuolation, multifocal in z. fasiculata (minimal)
Vacuolation, multifocal in z. fasiculata (minimal)
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Test Article: telomer alcohol/telomer acrylate/ /pfhs/pfos
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. Propath no. Date
242933 99010
21.Apr.1999
Individual Animal Data Records
Animal No: SEX: DOSE GROUP:
88 Kale PFOS
MICROSCOPIC FINDINGS (continued)
Spleen
Thymus
Animal No:
89
MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS Liver
Animal No:
90
MACROSCOPIC FINDINGS
liver
.-
Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (minimal)
Lymphoid atrophy - involution (minimal)
No abnormalities found in: Kidney/1, Kidney/2, Testes/1, Testes/2, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2.
.
days of treatment :29
sacrifice group
:recovery group
necropsy status
:planned recovery
date of start of treatment: 06/10/98
date of end of treatment: 03/11/98
date of necropsy :18/11/98
No findings noted
Inflammatory cell foci (slight)
Single cell necrosis (minimal) Midzonal/centrllobular hypertrophy, diffuse (slight)
Hepatocellular vacuolation (minimal)
days of treatment : 29
sacrifice group
: recovery group
necropsy status
: planned recovery
date of start of treatment: 06/10/98
date of end of treatment: 03/11/98
date of necropsy : 18/11/98
Accentuated lobular pattern
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Test Article: telomer alcohol/telomer acrylate/ /pfhs/pfos
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. Propath no. Date
242933 99010
21.Apr.1999
Individual Animal Data Records
Animal No: SEX: DOSE GROUP:
90 Male PFOS
M ACROSCOPIC FIND IN GS
(continued)
MICROSCOPIC FINDINGS Liver
Animal No:
91
MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS Liver
Animal No:
93
MACROSCOPIC FINDINGS
.
No abnormalities were found in any of the other tissues examined
Inflammatory cell foci (slight) Single cell necrosis (minimal) Midzonal/centrilobular hypertrophy, diffuse
(moderate) (correlates to GROSS finding) Hepatocellular vacuolation (slight) (correlates to
GROSS finding)
days of treatment :29
sacrifice group
:recovery group
necropsy status
:planned recovery
date of start of treatment: 06/10/98
date of end of treatment: 03/11/98
date of necropsy :18/11/98
No findings noted
Inflammatory cell foci (slight)
Midzonal/centrilobular hypertrophy, diffuse
(moderate)
'
Hepatocellular vacuolation (slight)
days of treatment : 29
sacrifice group
: recovery group 2
necropsy status
:planned recovery 2
date of start of treatment: 06/10/98
date of end of treatment: 03/11/98
date of necropsy : 02/12/98
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Test Article: telomer alcohol/telomer acrylate/ ,/pfhs/pfos
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NO.TOX no. Propath no. Date
242933 99010
21.Apr.1999
Individual Animal Data Records
Animal No: SEX: DOSE GROUP:
93 Male PFOS
MACROSCOPIC FINDINGS (continued)
MICROSCOPIC FINDINGS Liver
Animal No: 94 MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS Liver
Animal No: 95 MACROSCOPIC FINDINGS
.
No findings noted
Inflammatory cell foci (minimal) Single cell necrosis (minimal) Midzonal/centrilobular hypertrophy, diffuse
(minimal)
days of treatment : 29
sacrifice group
: recovery group 2
necropsy status
: planned recovery 2
date of start of treatment: 06/10/98
date of end of treatment: 03/11/98
date of necropsy : 02/12/98
No findings noted
Inflammatory cell foci (slight)
Midzonal/centrilobular hypertrophy, diffuse (moderate)
Hepatocellular vacuolation (minimal)
days of treatment : 29
sacrifice group
: recovery group 2
necropsy status
:planned recovery 2
date of start of treatment: 06/10/98
date of end of treatment: 03/11/98
date of necropsy : 02/12/98
No findings noted
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Page 138/146
Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ ,'PFHS/PFOS
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NQTOX no. :
242933
Propath no. :
99010
Date
: 21.Apr.1999
Individual Animal Data Records
Animal No:
SEX:
DOSE GROUP:
95
M ale
PFOS
M ICROSCOPIC FINDINGS
Liver ,
Animal No: SEX:
177 Female
M ACROSCOPIC FIND IN GS
'
MICROSCOPIC FINDINGS Liver
.
Kidney/2 Adrenal Gland/1 Spleen
Thym us
Inflammatory cell foci (alight) Single cell necroaia (minimal) Midzonal/centrilobular hypertrophy, diffuse (alight)
days of treatment : 28
sacrifice group
:terminal sacrifice
necropsy status
:planned terminal
date of start of treatment: 08/10/98
date of end of treatment: 04/11/98
date of necropsy :05/11/98
No findings noted
Inflammatory cell foci (slight) Single cell necrosis (minimal) Midzonal/centrilobular hypertrophy, diffuse (slight)
Tubular atrophy, cortical (minimal)
Extracapsular nodule present
Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (slight)
L ym ph oid a tr o p h y - i n v o lu t io n (m in im al)
No abnormalities found in: Pancreas, Kidney/1, Ovary/1, Ovary/2, Adrenal Gland/2, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2.
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Test Articles telomer alcohol/telomer acrylate/ ,/pfhs/pfos
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NQTOX no. Propath no. Date
242933 9901
21.Apr.1999
Individual Animal Data Records
Animal No: SEX: DOSE GROUP:
178 Female PFOS
MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS Liver
Kidney/2
Adrenal Gland/1 Spleen
Animal No:
179
MACROSCOPIC FINDINGS
days of treatment : 28
sacrifice group
: terminal sacrifice
necropsy status
: planned terminal
date of start of treatment: 08/10/98
date of end of treatment: 04/11/98
date of necropsy : 05/11/98
No findings noted
Inflammatory cell foci (minimal) Midzonal/centrilobular hypertrophy, diffuse (slight)
Tubular atrophy, cortical (minimal) Interstitial inflammation, lymphocytic (minimal)
Small glands, normal morphology.
Hemosiderin pigment (minimal)
No abnormalities found in: Pancreas, Kidney/1, Ovary/1, Ovary/2, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2.
days of treatment : 28
sacrifice group
: terminal sacrifice
necropsy status
:planned terminal
date of start of treatment: 08/10/98
date of end of treatment: 04/11/98
date of necropsy : 05/11/98
No findings noted
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Page 140/146
Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ VPFHS/E'FOS
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. Propath no. Date
242933 99010
21.Apr.1999
Individual Animal Data Records
Animal No: SEX: DOSE GROUP:
179 Female PFOS
MICROSCOPIC FINDINGS
Liver
Spleen
Animal No: 180 MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS Liver
Kidney/1 Spleen
-
Inflammatory cell foci (alight) Single cell necroaia (minimal) Midzonal/centrilobular hypertrophy, diffuae
(moderate)
Hemosiderin pigment (moderate)
No abnormalities found in: Pancreas, Kidney/1, Kidney/2, Ovary/1, Ovary/2, Adrenal Gland/1, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2.
days of treatment : 28
sacrifice group
: terminal sacrifice
necropsy status
: planned terminal
date of start of treatment: 08/10/98
date of end of treatment: 04/11/98
date of necropsy : 05/11/98
No findings noted
Inflammatory cell foci (slight) Midzonal/centrilobular hypertrophy, diffuse
(minimal)
Interstitial inflammation, lymphocytic (minimal)
Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (slight)
No abnormalities found in: Pancreas, Kidney/2, Ovary/1, Ovary/2, Adrenal Gland/1, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2.
BACK TO MAIN
PATHOLOGX REPORT
.
Page 141/146
Test Article: tslomer alcohol/telomer acrylate/ -/pfhs/pfos
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. Propath no. Date
242933 99010
21.Apr.1999
Individual Animal Data Records
Animal No: SEX: DOSE GROUP:
181 female PFOS
MACROSCOPIC FINDINGS
M ICRO SCO PIC FINDINGS
Liver
Kidney/1 Spleen
Animal No:
182
MACROSCOPIC FINDINGS
days of treatment : 29
sacrifice group : terminal sacrifice
necropsy status
: planned terminal
date of start of treatment: 08/10/98
date of end of treatment: 05/11/98
date of necropsy : 06/11/98
NO findings noted
Inflammatory cell foci (minimal) Single cell necrosis (minimal) Midzonal/centrilobular hypertrophy, diffuse (slight)
Tubular atrophy, cortical (minimal)
Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (moderate)
No abnormalities found in: Pancreas, Kidney/2, Ovary/1, Ovary/2, Adrenal Gland/1, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/1, Mandibular Lymph Node/2.
days of treatment : 29
sacrifice group
: terminal sacrifice
necropsy status
:planned terminal
date of start of treatment: 08/10/98
date of end of treatment: 05/11/98
date of necropsy : 06/11/98
No findings noted
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PATHOLOGY REPORT
Page 142/146
Test Article: telomer alcohol/telomer acrylate/ /pfhs/pfos
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NQTOX no. Propath no. Date
242933 99010
21.Apr.1999
Individual Animal Data Records
Animal Ho: SEX: DOSE GROUP:
182 Female PFOS
MICROSCOPIC FINDINGS
Liver
Kidney/1 Spleen
Mandibular Lymph Node/ 1
Animal No:
183
MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS Liver
Kidney/1 Spleen Mandibular Lymph Node/
1
Inflammatory cell foci (minimal) Midzonal/centrilobular hypertrophy, diffuse
(moderate)
Tubular atrophy, cortical (minimal)
Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (slight)
Plasmacytosis (slight)
No abnormalities found in: Pancreas, Kidney/2, Ovary/1, Ovary/2, Adrenal Gland/1, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/2.
days of treatment :29
sacrifice group
:terminal sacrifice
necropsy status
:planned terminal
date of start of treatment: 08/10/98
date of end of treatment: 05/11/98
date of necropsy :06/11/98
No findings noted
Inflammatory cell foci (minimal) Single cell necrosis (minimal) Midzonal/centrilobular hypertrophy, diffuse
(moderate)
Interstitial inflammation, lymphocytic (minimal)
Hemosiderin pigment (slight)
Plasmacytosis (slight)
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PATHOLOGY REPORT
Page 143/146
Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ j/PFHS/PFOS
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. :
242933
Propath no. :
99010
Date
: 21.Apr.1999
Individual Animal Data Records
Animal No: SEX: DOSE GROUP:
183 Female PFOS
MICROSCOPIC FINDINGS (continued)
Animal No:
184
MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS Liver
Kidney/1 Spleen
'
No abnormalities found in: Pancreas, Kidney/2, Ovary/1, Ovary/2, Adrenal Gland/1, Adrenal Gland/2, Thymus, Mesenteric Lymph Node, Mandibular Lymph Node/2.
days of treatment : 29
sacrifice group
: terminal sacrifice
necropsy status
:planned terminal
date of start of treatment: 08/10/98
date of end of treatment: 05/11/98
date of necropsy : 06/11/98
No findings noted
Inflammatory cell foci (minimal) Midzonal/centrilobular hypertrophy, diffuse (slight)
Tubular atrophy, cortical (minimal)
Hemopoiesis, primarily erythropoiesis (minimal) Hemosiderin pigment (slight)
No abnormalities found in: Pancreas,Kidney/2,
Ovary/1, Ovary/2, Adrenal Gland/1, Adrenal Gland/2,
Thymus, Mesenteric Lynph Node, Mandibular Lymph
Node/1, Mandibular Lymph Node/2.
.
BACK TO MAIN
PATHOLOGY REPORT
Page 144/146
Test Article: TELOMER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PFOS
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NOTOX no. Propath no. Date
242933
99010 21 Apr.1999
Individual Animal Data Records
Animal No: SEX: DOSE GROUP:
185 Female PFOS
MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS
Liver
Animal No:
186
MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS
Liver
.
Animal No:
187
MACROSCOPIC FINDINGS
-
days of treatment : 29
sacrifice group
: recovery group
necropsy status
: planned recovery
date of start of treatment: 08/10/98
date of end of treatment: 05/11/98
date of necropsy : 20/11/98
No findings noted
Inflammatory cell foci (minimal)
days of treatment : 29
sacrifice group
: recovery group
necropsy status
: planned recovery
date of start of treatment: 08/10/98
date of end of treatment: 05/11/98
date of necropsy
: 20/11/98
No findings noted
Inflammatory cell foci (minimal) Midzonal/centrilobular hypertrophy, diffuse
(minimal)
,
days of treatment :29
sacrifice group
:recovery group
necropsy status
:planned recovery
date of start of treatment: 08/10/98
date of end of treatment: 05/11/98
date of necropsy
:20/11/98
BACK TO MAIN
PATHOLOGY REPORT
Page 145/146
T e s t A r t i c l e : telomer alcohol/ telomer acrylate/
/ pfhs/ pfos
Test System : Exploratory 28-Day Gavage Study in Rats
Sponsor
: 3M Toxicology Services
NQTOX no. Propath no. Date
242933 99010
21.Apr.1999
Individual Animal Data Records
Animal No: SEX: DOSE GROUP:
187 Female PFOS
MACROSCOPIC FINDINGS (continued)
MICROSCOPIC FINDINGS
Liver .
Animal No:
189
MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS Liver
Animal No:
190
MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS Liver
No findings noted
Inflammatory cell foci (slight) Single cell necrosis (minimal)
days of treatment : 29
sacrifice group
: recovery group 2
necropsy status
:planned recovery 2
date of start of treatment: 08/10/98
date of end of treatment: 05/11/98
date of necropsy
: 04/12/98
No findings noted
Inflammatory cell foci (slight) Single cell necrosis (minimal)
days of treatment : 29
sacrifice group
: recovery group 2
necropsy status
:planned recovery 2
date of start of treatment: 08/10/98
date of end of treatment: 05/11/98
date of necropsy
: 04/12/98
No findings noted
.
Inflammatory cell foci (minimal) Single cell necrosis (minimal)
BACK TO MAIN
PATHOLOGY REPORT
T e s t A r t i c l e : telomer alcohol/telomer acrylate/ ./pfhs/pfos
T e s t S y s t e m : E x p l o r a t o r y 2 8 - D a y G a v a g e S t u d y iri R a t s
Sponsor
: 3M Toxicology Services
Page 146/146
N O TOX no. Eropath no. Date
242933 99010
2 1 .Apr.1999
Individual Animal Data Records
Animal No:
SEX: DOSE GROUP:
191
Female PFOS
MACROSCOPIC FINDINGS
MICROSCOPIC FINDINGS Liver
days of treatment : 29
sacrifice group
: recovery group 2
necropsy status
:planned recovery 2
date of start of treatment: 08/10/98
date of end of treatment: 05/11/98
date of necropsy
: 04/12/98
No findings noted
Inflammatory cell foci (minimal)
Midzonal/centrilobular hypertrophy, diffuse (minimal)
3M Environm ental Technology and Services
PO Box 33331 ~ St. Paul, MN 55133-3331 612 778 6442 *
BACK TO MAIN
3M
j
I i
Laboratory Report Report of Data for Exploratory 28-Day Oral Toxicity Study in Rats: Telomer Alcohol, Telomer Acrylate, , PFHS, PFOS
Laboratory Report No. FACT-TOX-120.3
Requester Project No. NOTOX #242933
Testing Laboratory
3M Environmental Technology & Safety Services 3M Environmental Laboratory
Fluorine Analytical Chemistry Team (FACT) 2-3E-09
935 Bush Avenue, S t Paul, M N 55106
,
Laboratory Contact
. . Kris Hansen, Ph.D. Bldg. 2-3E-09 P.O .B ox 3331
S t Paul, M N 55133-3331 Phone: (651)778-6018 FAX: (651) 778-6176
Requester M arv Case and Paul Lieder
3M Company Toxicology Sendees '
220-2E-02 S t Paul, M N
Report issued 6/ 11/99
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3M ENVIRONMENTAL LABORATORY REPORTNO. FACT-TOX-J20
This study was designed to preliminarily assess the toxicity o fvarious test articles to the female Sprague Dawley r a t Test animals were dosed by oral gavage at the M ow ing levels:
Group 2: Telomer Alcohol
Group 3: Telomer Acrylate
Group 4:
Group 5: PFHS
G roup6:PFOS
30m g/kg/day
30 mg/kg/day
30 mg/kg/day lOmg/kg/day 3 mg/kg/day
W ithin each group, 8 anim als were given test material each day for 28 days, and then sacrificed. Three
anim als were allowed to "recover" (no test material) for 14 days before sacrifice; three animals were allowed
to recover for 28 days before sacrifice.
.
Liver samples collected from the sacrificed animals were sent to the Environmental Lab and analyzed quantitatively for perfluorooctane sulfonate (PFOS) and other perfiuorinated, sulfonate-based or carboxylic ad d based metabolites. (A M list o f acronyms and formulas is attached in Attachment B).
Tissue samples were homogenized (liver) and extracted using an ion-pairing extraction procedure. The .extracts were quantitatively analyzed using high-pressure liquid chromatogiaphy/electrospray tandem mass spectrometry (HLPC/ESMSMS), and evaluated versus an extracted curve. Analytical details are available in the study binder maintained by FACT. The presence or absence ofknown fluorochemical contaminants and metabolites is determined by inspection.
Six perfiuorinated carboxylic adds were identified in the liver sample extracts derived from both group 2 and
grog) 3 animals (see Attachment D).
.
Forgroup2 and group 3, livers from male and female animals in both groups ofrecovery animals showed a
. decrease in the concentration ofPOAA The concentration o f two other carboxylic ad d based
.
fluorochemicals measured quantitatively (M513, M513) did not show as significant a decrease over the
recovery period. The concentrations ofthree other carboxylic add-based compounds could notbe
determined, as standard material is not available. Other perfiuorinated or partialfy-fluorinated metabolites
may be present in the rat liver samples analyzed, but these compounds cannot be confirmed without standard
m aterial
.
Relative to PFOS levels in the corresponding group 6 animals, (grog) 4) and PFHS (grog) 5) levels
determined in liver samples showed a dramatic decrease in the day 14 and day28 recovery groups. W ithin
each group, samples showed a relatively large standard deviation which may indicate variability in dosing or
in metabolism o f the test compound.
's
W ith the exception ofPFOS-dosed animals (group 6), there were dramatic differences in target analyte levels
in livers o f m ale and fem ale animate For example, livers from 14 day recovery group 5 females animals
were determined to contain two and a halforders o f magnitude less PFHS than the corresponding male
animals.
.
Although rigorous quality control measures and 3MEnvironmentaI Laboratory Standard Operating Procedures were M ow ed, die acquisition of this data was not necessarily collected according to applicable Good Laboratory Practices. This data is not suitable for distribution to ary governmentagency in defense of
product suitability or safety. Data presented here is the highest quality data available at this time.
This summary should not be considered to be a complete inventory of all metabolites associated with the test
m aterial Analyte measurements were made only on those compounds deemed by FACT as likely to be
. present Certain other compounds have been identified but cannot be quantitated as standard material is not
available.
.
BACK TO MAIN
3M ENVIRONMENTALLABORATORY REPORTNO. FACT-TOX-120
Liver samples arrived frozen at the Environmental Lab on 2/3/99. A chain-of-custody was not submitted with the samples, but was initiated upon receipt by die Environmental Lab.
1
]
A nalytical m ethods
1 FACT-M-l.Q, Extraction o fPotassium Per&uooctanesulfbnate or Other Anionic Fhiarochemical
Surfactants from Liver for Analysis Using HPLC/ESMS
.
FACT-M-2.0, Analysis ofFluorochemicals in LiverExtract Using HPLC-EIectrospray/Mass Spectrometry
Sam ple p rep aratio n -aq u eo u s sam ples, HPLC7ESMSMS: ion-pairing extraction .
.
*. Analyte is extracted from a sample matrix with ion pairing reagent [tetrabutyl ammonium hydrogen sulfate
(TBA)] in a pH-controlled environment The cationic reagent selectively targets anionic fluorochemicals.
Once the anion-TBA pair is formed, the analyte is transferred into a non-polar organic solvent (m ethyl-tert-
butyl ether), dried, and reconstituted in methanol for MS analysis.
HPLC/ESM S and HPLC/ESM SM S: fo r detailed qualitative woric . In HPLC, an aliquot of extract is injected and passed through a reverse phase liquid chromatographic
column. Based on the affinity o f the analyte for the stationary phase in the column relative to the liquid . mobile phase, die analyte is retained for a characteristic amount of time. For example, in a standard 1 solution PFOS may elute at 5.0 minutes. Retention limes between a standard PFOS solution and the analyte extracted ftom liver in this analysis were matched on the HPLC system to within 1%. ..
J3 Following HPLC separation, ESMSMS provides a rapid and accurate means for analyzing a wide range of organic compounds, including fluorochemicals. Electrospray, an ionization technique used primarily for detection o f molecular ions, is generally operated at relatively m ild temperatures. Molecules are ionized, and a primary ion, characteristic of the analyte, is selected. This ion is bombarded with high-energy gas; subsequent collisions create smaller secondary ionic fragments unique to the primary ion, which are
detected.
v
For example, for PFOS (CgFpSQO analysis, ion 499 is selected as the characteristic primary ion. This ion
] is fragmented into other ions such as 80 amu (corresponding to SO3"), and 230 atm (C3F6SCV). Each o f these secondary fragm ents is detected and can be used to differentiate PFOS from other compounds that
might have the same characteristic 499 amu primary ion, but different chemical compositions and
secondary ion fragmentation patterns.
I
5.1 Calibration
J A mid-level, extracted matrix calibration check standard is analyzed every 5-10 samples to monitor instrumental drift Calibration check standards are compliant if instrumental response is within +/- 30% o f the expected value.
i
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1 I 3MENVIRONMENTAL LABORATORY
REPORTNO. FACT-TOX-120
Quantitation of the target analytes is based on linear regression analysis (weighted 1/x, if appropriate) o f
two extracted matrix curves bracketing each group of samples. Quantitation of each analyte is based in the
response of one or more specific daughter ions using the multiple response-monitoring mode o f the
instrum ent
.
5.2 Blanks
Two extraction blanks, utilizing water as surrogate matrix, are extracted with each batch of sanqdes. Additionally, two samples ofblank matrix; used for the extracted calibration curves, are extracted along w ith each batch of samples and curves.
Blanks are compliant if no target analyte is detected above the limit ofdetection for a specific analyte. In
this study, all blanks were compliant .
`
5.3 Surrogates
.'
Tetra-hydro perfluorooctane sulfonate is used as a surrogate in this analysis. Surrogate response is monitored to confirm gross instrum ental failure, if necessary.
5.4 Matrix Spikes
Duplicate matrix spike analyses utilizing all target analytes were prepared and analyzed for one control animal. Except as noted in the results table, recoveries were within foe acceptable range o f 70-130%. Recoveries outside this range may be re-evaluated.
5.5 Laboratory Control Samples
Laboratory Control Samples are not a component o f this study.
! Six perfluorinated carboxylic adds were identified in the liver sample extracts derived from both Group 2 and
Group3 animals (see Attachment D).
'
J For group2 and group 3, livers from male and female animals in both groups ofrecovery animals showed a decrease in the concentration o fPOAA. The concentration o ftwo other carboxylic arid based fluorochemicals measured quantitatively (M513, MS13) did not show as significant a decrease over the
' recovery period. The concentrations of three other carboxylic arid-based compounds could not be
j determined, as standard material is not available. Other perfluorinated or partially-fluorinated metabolites may be present in the rat liver samples analyzed, but these compounds cannotbe confirmed without standard
m aterial.
x
Relative to PFOS levels in the corresponding group 6 animals,
(group 4) and RFHS (group 5) levels
] determ ined in liver samples showed a dramatic decrease in the day 14 and day 28 recovery groups. W ithin
each group, samples showed a relatively large standard deviation which may indicate variability in dosing or
in metabolism ofthe test compound.
.
W ith the exception ofPFOS-dosed animals (group 6), there were dramatic differences in target analyte levels
in livers o f m ale and female animals. For example, livers from 14 day recovery group 5 females animals
were determined to contain two and a halforders ofmagnitude less PFH5 than the corresponding male
anim als
.
.j
These results indicate that ingestion of each o fthe test materials leads to some accumulation o f metabolites in the liver. Relative rate ofclearance can be determined.
BACK TO MAIN
3MENVIRONMENTAL LABORATORY REPORTNO. FACT-TOX-120
Samples and extracts willbe retained for future evaluation, if necessary.
9.1 A ttachm ent A : Table of Results o f Analysis o f L iver Samples
9.2 A ttachm ent B : Table of FACT acronyms and form ulas
9.3 A ttachm ent C : Graphical Results by Group and Gender
9.4 A ttachm ent D: Chromatographic results ofPFC A investigation in Group2 and Group 3
9.5 A ttachm ent E : Chain o f Custody
.
9.6 A ttachm ent F : Analytical Data
.'
LisaClemen,AnalyticalChemist
Date
/ 135----- -------------- `________ 6//
Kris Hansen,P hD , Principal Analytical Investigator
D ate
.x
3 M Environm ental Laboratory Fluorine A nalytical Chemistry Team fFACT)
FACT-TOX-120, R at Livers 28-D ay O ra! Tox Study by D aily Gavage in Rats
1
i PFOS (C8F 17S03`)
1
(Female)
Day 1
Day 14
Day28
G l 0.086+/-0.046 0.102+/-0.020 0.329+/-0.060
1
G6
377+/-31
240+/-25
191+/-15
]
(Male) Gl
Day 1
Day 14
Day28
0.133+/-0.030 0.255 +/- 0.122 0.099+/-0.007
G6
4 1 9 + /-8 6
3 0 9 + /-3 4
237+/-25
1
I PFHS (C6F I3S03-)
(Female)
Day 1
Day 14
Day28
j
Gl
<MDL
<MDL
<MDL
G5 97.0 +/-14.7 0.26+/-0.12 0.03 +/- 0.01
I
(Male)
Day 1
Day 14
Day28
G l 0.055 +/- 0.020 0.129 +/- 0.088 0.089+/-0.019
] G5 327+/-52 61.9+/-11.7 36.3+/-7.4
J
3 ( /
(Female)
D ayl
Day 14
Day28
J Gl
<MDL
<MDL
<MDL
G4
0.37+/-0.38 0.02+/-0.00
<MDL
1
(Male)
Day 1
Day 14
Day28
! Gl
<MDL
< MDL
<MDL
G4 3.09+/-1.58 0.126+/-0.084 0.025 +/- 0.015
I
j 3M Confidential
BACK TO MAIN
3 M Environm ental Laboratory Fluorine A nalytical Chemistry Team fFACTl
FACT-TOX-120, R at Livers 28-D ay O rai Tox Study by D aily Gavage in Rats
POAA (C7F 1SC02)
(Female) Gl G2 G3
(Male) Gl G2 G3
Dayl <MDL 0.164+/-0.035 0.112+/-0.039
Day 14
<MDL
0.016 +/-0.010
< MDL
Day28
<MDL <MDL <MDL
Dayl <MDL 6 .5 9 + /-2 .1 6 8.64+/-1.48
Day 14 <MDL 3 .5 9 + /-0 .6 7 3.36+/-1.65
Day28 <MDL 1.76+/-0.23 1 .2 6 + /-0 .4 4
MS13 (C,F19C02)
(Female) Gl G2 G3
(Male) Gl G2 G3
Dayl <MDL 1.69+/-0.18 1.20 +/-0.30
Day 1 <MDL 1.91+/-0.88 1.78+/-0.31
Day 14 < MDL 1.36+/-0.02 1.20+/-0.09
Day28 <MDL . 2.24+/-0.22 0.812+/-0.188
Day 14 <MDL 1.73+/-0.46 1.08 +/- 0.26
Day28 <MDL 1.52+/-0.32 0.801+/-0.148
M613 (Cn F 23C02-)
(Female) Gl G2 G3 .
(Male) Gl G2 G3
. Day 1 <MDL
0.117+/-0.020 0.029 +/- 0.017
Day 14 <MDL 0.085 +/-0.019 0.035 +/-O015
Day28 <MDL 0.101+/-0.012 0.036+/-0.011
Day 1 <MDL 0.087+/-0.015
0.0622
Day 14 <MDL 0.107+/-0.041 <MDL
Day28 <MDL 0.106+/-0.017 <MDL
* * C9, C ll and C13 perfluorinated carboxylic acids also detected in G2 and G3 animals 3M Confidential
BACK TO MAIN* L.
28-Dsy O rai Tox Stady by DaUy Gavage In Rata
Stady: frodaci Nttrti ufTcn Sakatmcc): Manta:
DatcoTExtractiaVAnalysC Data o f Asalyab/Ajulytt: Otte * f Data Radaction/AMlyM
SampIcDaU
TOX-I21 Exflomory 29-Day Orai Teoddty Slady by Dafly Garage fa latti
T-7023, T-7024, T-7022, T-7032, T-62W
MMUva
Filrnnar
FACT-M-I.O k FACT-M-2.0
K-Sqund Vaine:
Sonp 020199, Madrine 04109, A ad Si 06349
Slopc
M taLynM
2/22/99 RWW/SAH
02/23/99, 03/01/99, 03/11/99, 03/25/99HOJ/MEE/DRB
02/25/99, 03/01/99, 03/12/99, 05/26/99 MEE/DRB/KJH/HQJ
SceAltacfaacais S e e A lU d n cate See Attachmcal* Sm Attachouatt
.
RATUVER
Cm f
S u *kl
Irtrtrtri W t
F rS
Telai Avana!
Dnac
1
Cale. Cane
afPPOS
rt rt
Mctbod Blk
H2 0 B&-I
NA
<MQL
<MDL
MtifcodBlk
H20 BIk-2
NA
<MDL
<MDL
Matrix Blk
TGSSTK b S T
HA
<MDL
<MDL .
Mania Blk Q C - 2 * *
Ratti! Uvcr Blk-2 I06P
HA 0.9240
<MDL 25
<MDL UH
I06P
0.9240
250
*4%
Q C - 250PI*
I 10P
0.9242
69
23%
tlOP
0.9242
99
33%
Cranpl
97F 03221 190 1190
0.0at|/k|/day Cannai
91F
0.4339
673
10672
99P 03106 76.1 10761
100P 039*4 64.9 10649
I01P
0.6370
9007
9.01
102P
0.4313
763 - 10765
103P 0.4961 653 10655
104P 0.1116 62.0 10620
Cranpl
105P
0.6936
119
1119
O.Oaw'kg/day
I06P
0.9240
toc
1106
Recovery 1- 14Daya
107P
0.4359 10.6 10606
G ranfi
109P
0.4UO
344
1344
lOmffkg/day
1IQP
0.9242
379
1379
Recavay 2-21 Daya
11IP
0.9429
263
1263
G ranfi
113P
0.3506
413
10412
30.0atfAg/day
IMF
0.6495 317 10337
T doacr Akohol
1ISP
0.6769 37.1 10371
u f f 0.7235 343 10366
1I7P 0.6534 11.6 1 M I6
h i p 0.4162 56.9 10569
1199 0.5791 310 10360
1209
0.4295
293
10295
G ranfi
121P 0.1372 35.6 10356
M Om fftg'dty
122P 06291 35.4 10354
Recovery 1 14Davi
123P
03660 <7.3 10473
Craapl
125P
0.6074
433
0.0452
30.0 B/kf/day Recovery 2 - 2 Daya
1269 I27P
0.96H 0.6066
515 510
10515 10520
G ranfi
129P . 014161
343
10342
30.0 mffkf/d*y Tdaner Aayiata
1309 I3IP
03012 0.7303
M .4 . 314
10364 10516
132P
0.5990
413
10415
1339
0.3111
494 . 10496
1349
0.6131
453 / 10452
1359
0.5169
317
10357
1369 03799 46.1 10461
G ranfi
1379 0.6064 17.9 10179
31 0nffka/My Recovery t - 14Daya
13BF 139P
0.6235 19.4 10194 0.5660 43.9 10439
G ranfi
I4IP
0.6693
414
10424
3 1 4 n ffk f/ d ay
I42P
0.7953
413
10411
Recovery 1 -21 Daya
143P
0.7363 403 10402
LA** 10a(/g,M513 -S O R 100nf/g, M613- 50a f ff
Metfand Detection Lfaak (MDL): F F O S -2 3 * f c F O A A - 5 a | fr MS13- 2S a a d S O ^ fe M 613-25afff
Avance rras art <MDL MDL 15% 26%
10063* 130 1102 0329
10443 10394 10519
10429 10271 10413
Data Entcrnd/Aariyat: Date Verified/Aaalyat:
02/26/99. 03/0C/99, 03/22/99. 05/2699 LAC
06M /99, 06/DW99 BAD
M
BSD SUL Dar.
<MDL <MDL
3% 36%
53.4* >10461*
263 3.15 193 10196 113 10397
36.6 10171
173 0.00664
12. 100667
15.0 100644
53.9 10146 2.70 100112
- POAA Caie. Cane.
Tata! Amoea! af POAA
Avance POAA
BSD SdDcv.
rt ...... <MDL
art <MDL
rt
<MDL
<MDL
<MDL
<MDL
<MDL
<MDL
<MDL
<MDL
<MDL
<MDL
219 73%
267 19% 1% 20%
222.90
75%
172.72 56% 66% 25%
0.00 <MDL 100 <MDL 0.00 <MDL
io o <MDL
100 100 100 0.00 0.00 0.00 0.00 100 0.00 100
<MDL <MDL <MDL <MDL <MDL <MDt <MDL <MDL <MDL <MDL
<MDL <MDL <MDL
<MDL <MDL <MDL
135 1135 115 111S 16) 1163
160 1160
115 1115 196 1196
223 0323
213
139
1139
1164
9.0954
24.3 10245
433 100453
653
17.4
10174
10153
10101
0 <MDL
0 <MDL
0
<MDL
<HDL
<MDL
663 10665
105 1105
95.0
10950
*
166 1166
124 1124
90.6 10906
170 1170
35.9
75.1
10751
1112
10390
100 <MDL
100 <MDL
0.00
<MDL
<MDL
<MDL
100 <MDL
100 <MDL
100
<MDL
<MDL
<MDL
* Val w u detonalaed ao collier and wac no! hcladcd a Icae cikalriiana.
N R -N o t Rqwrtcd
N /A-N ot Applkabl
PFOS-Ftfflaoraoctaaaalfi
POAA PcfflaoroocHaoHc
M513- C 9P19COO-
M613 CI IF23000-
Cale. Cane.-
rt <MDL <MDL <MDL <MDL
122 193 122 193 100 0.00 6VALUEI 100 100 0.00 . IVALUet VALUEI 6VALUEI VALUEI VALUEI VALUEI VALUEI VALUEI
VALUEI
VALUEI
VALUEI VALUEI*
VALUEI VALUEI
VALUEI VALUEI VALUEI
VALUEI
VALUEI VALUEI VALUEI VALUEI
(VALUEI VALUEI VALUEI VALUEI VALUEI VALUEI VALUEI VALUEI VALUEI
VALUEI VALUEI VALUEI VALUEI VALUEI
'
Tata! Amena! af
art <MDL <MDL <MDL <MDL 41% 65% 41% 65%
io PI*
<lpp HR
<ippk
I0 PP* <10 PI*
NR
HR HR HR HR HR HR HR
HR NR NR NR
NR NR NR NR NR
NR NR NR NR NR
NR NR NR NR NR NR NR NR NR NR NR NR NR NR
Avance rt <MDL <MDL 53% 53%
NR NR NR
NR NR NR
NR NR NR
RSD ftd.Dev.
<MDL <MDL
46% 49%
NR NR NR
NR NR NR
NR NR NR
PACT-M-2.0 7.0
TOX42 1livm.xh
4/1099 5:12 9*4
***** frodiciH i te (ra> M w a ) :
Macrc
AnalytkalEyytSyncroKwkB
DMcerExtnctWABilyst: DatecfAaalyw/Aeaiy*: DMcofDauRcdcctioa/AnfyaC
SuapleData
FACT-TOX-120
2 W J y O ra i Tox S tody by DUy G ir a te fai R i t i
TOX-120,Expfcntory2t-DayOraiToridcyStady9yDeflyQnra|tiaRata
T-7023,T-7024,T-7022,T-7032,T-6295
RrtJJwr
SeItalotifi*
FACT-M-1.0 k FACT-M-2.0
R-SquredVatoeScfAtlachmcatt
SMp 0201, lladdM 04I0W; Aactia 00491
M anLywl!
imm rww/sah
v a r a n e ov o i m , ov u m , o s a s m hoj/meb/d rb
a i m m , ovoc/*, o v ia m , t a n t m mee/drb/k jh h o j
BACK TO MAIN
RATUVER P ro
MdhoORik IM ifallk QC-250pp4 QC-250ppfc
0.0 M$fc$/dMy Cootrol
.om$rkttf Recovery i . 14 D m
Graap | <20a*/k*/d*y Recovery 2-21 D m SO.Oaf'ksMay Tdorocr Alcohol
30.0*k*/day Recovery 1 14 D m
GrmpZ SO.Oroftyday Recovery 2-2* D m 3R0 **d*y Tdoacr Aoytaia
J0.0*A**ay Recovery 1 - 14 D m
.O o iM iy Recovery 2-21 D m
UmpUI
H20 Blk-1 H20Wk-2 M M lL lnrB ST Rabhb Uver Blk-2
I06F 1I0P 110F 97F nr 99T loop* 10IP 102F 103F IMF I0SF 106F 107F 109F 110F I11F I13P 1UF 115F 116F 1I7F
h ip
UFF 120P 12IF 122F 123F I25F 126F 127F
IMF 1JIF IMF IMF IMF IMF IMF 137F 13IF 139F I41F 142P wr
1
NA NA NA NA 0.9240 09240 0.9242 0.9242 05221 0.4339 OJIO 0.59*4 0.6370 0.4313 0.49*1 0*11 0.6934 0.9240 0.4359 0.41*0 0.9242 0.9429
0.4495 0.4769 0.7235 0.6534 04162 09791 04295 0.1372 0.6291 09660 0*074 0.9*12 060*6
0.3012 07303 09990 0JI11 0.4131 0.536* 09799 0*0*4 06239 096*0 0.6693 07993 07319
CMc. Cea. 0%
i. ~,*MDl.; <MDL '<
....
. " ' ^ ' <MDL -, '' . I M O
'flOdl
W $ ii - ?i,V 0t00^*
v '.^hOOOt^- v*. l* 3 m * * M * ^ i^ f^ T O tfiSF & J S S S S
lillii
;JM o o % .n ?
* '1
aCPFM*
n /i *MDL <MDL <MDL MDL 21494 94194
OS OS
NR NR NR
NR NR NR NR NR
NR . NR NO NR NR NR
NR NR NR NR NR NR NR NR
NR NR NR NR NR NR
NR NR NR NR NR NR NR NR NR NR NR NR NR NR
.
FFH *fr <MDL <MDL 36694 OS
NR NR NR
NR NR NR
NR NR NR
DataEatered/Auly: ouitm,0300/99,mmm,05/24/99la DateVaifiaMntyat: OMM/99,069*99EAD
RSD StODev. <MDt <MDL
IOS *DIV/(H
NR NR NR
NR NR NR
NR NR NR
M51J Cale. Ceac
> rt <MDL <MDL MDL <MDL
274 212 179 162 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.0 0.00 0.00 0.00
1791 ' 1*16
1671 1556 1353 164! 171* 1931 1366 1361 2396 2323 1991 1015 1446 72 1444 7*5 1123 1646 1252 119* 1302 1113 1014 7*2 640
(H ill V i <MDL
<MDL <MDL MDL 92S 9494 . 6094 54% <MDL <MDL <MDL <MDL <MDL 04DL <MDL <MDL <MDL <MDL <MDL <MDL <MDL
:-:;1-W 7 'W
ts $ {
140 133 1.99
1.01 1.45 0.(72 1.44 0.7*5 1.12 1.05 I.2S
IJO L30 1.11 1.01 0.7*2
.
Averti* MS13 a <MDL <MDL 93% 97S
<UDL
<MDL
<MDL * o a* **
L69 * 00
U6
2J4
IJO
1J*
0.112
NR-NetReportad WA--NotApplfeaM* FFOS-Ferfkorooctroaal
FOAA-PcrIUorooctiooaU M51J - C9F19COO-
M413 - CI 1P23COO-
"fasi H tn
<MDL <MDL
2% 10%
MDL
<UDL
<MDt
I0L7 Olili 1.12 0.02 f.9 OJIO
213 OJOS 7.17 0.0941 23J OJIS
- 'W ii Clk.CMC.
Vi <MDL
MDt <MDL <MDL
267 314 272 241 0.00 0.00 0.00
0.00 M 0.00 &00 0.00 0.00 OiOO 000
aoo 0.00
Tefal Aroatei ' ef MfU
<MDL <MDL <MDL
19% 105% 91% 1% <MDL <MDL <MDL <MDL <MDL <MDL <MDL <MDL <MDL <MDL <MDL <MDL <MDt
142 121 14
112 120 109
106 *3. 101 <SJ >9.0
974 114 90.5
213 32J 27J 30.7 4.74 644 25.9 22.7
19J 36.4
49.7 43J
4U 23.1
0.142 0.121 * 0.14 0.112 0il20 aios Olio 0.0*36 0.103 0.06S5 00*50 0.0974 0.114
Q.Q2t9 0.0321 0.0272 0.0307 0.0047 ' 0.0644 0.0259
0.0(93 0.0364 .M97 0.0433 OlMIS 010231
Avcrai M6I3
aii7 01004 noi
0.0351 0.0361
uso StO. Dcv.
12%
I7J 22.3 11.1 &0119
51.2 43J 29.7 0.0107
FCT-M-2.0
PwrfVenJaiT#
TOX-120-tvw.xl*
*nom
5:12m
----------- FAC---------,<-110 , 28-Day O ral Tax Study by Dally G iv a te la R ati
M a d N a n b a tfc * SnWmce):
HcAod/RcvUa: Analytical Equipment SyUM N n A as
Date of Extraction/Aailyit: DMeof Aulym/Aaalyat: Dato of Data Rcdaction/Aaalyst:
Sam ple Data
TOX-120, Exploratory 24-Day Oral Toxidty Stady by Dafly Gavajg a Kata
T-7023. T-7024, T-7022, T-7032, T-4295
Rat liver
FACT-M-t.O k FACT-M-XO
R-Sqaand Vaine
Madetiat 041091, Amelia 0C49I, md Soap 020199
Slope
MaacLyax3.l
Y-btorccpC
1/22/99 rw w /sah
02/24/99, 03/1 \ m , a v i 4/99. 05/25/99 h oj/m e e /d rb
03/0099,01/12/99,' 03/17/99, 05/26/99 DRB/KJH/HOI
RAT LIVER Cmp Dam
Saaplef
Extracted WL S
Method Bit Method Blk Matrix BU Matrix. Blk QC-lM pp*
iJ-iM pi*
Cranp4
Cm p4 30.0 np'kg/day
Recovery 1 - 14 Day
Graap4
30.0 Bg/kf/day
Recovery 2 >21 Day*
C r ,l
"
10.0 B|/kf/tfqr
PPHS
Croups 10.0 Bf/kf/diy Recovery I - 14 Day*
GroopS 10.0aifAfUay Recovery 2 -21 Deyi
C rm p4 3.0 awtyUay
pros
C napf 3.0 np/kg/dsy
Recovery 1-14 Daya Groop 4
).OH0ka/day Recovery 2 21 Days
K20 Blk-1
H20 Btk-2 Rebbit Liver Blk-1 Rabbit LiwBlk-2
106P I06P HOP nor 14SF 144P I47P I4IP 149P HOP
HIP H2P 153P 1S4P 133P 137P
HIP 139P
161P l2P
I43P 14P TOP I66P 147P
IMP 169F 170P
171P 173F 174P 173F 177P
I7IP 179P HOP HIP H2P TOP IS4F IMP IMP H7F IMP 190P
191P
HA HA HA HA 0.9240 09240
09242 09242
0.3643 04113 04457 04499 04097 04414 0.5474
04341 0.1374 07221 01019
O lili 0.5430 0.9194
09199 06444 04107 04779 07719 0.4252 04732 04374 05394 07757 0.5471 0.4764 0.7937 04424
07152 04423 05462 01106 J im 05940 04)12 04207 1.0570 0.4345 07564 04749 04442 09140
PFOS
Cale. Cne.
n ft <MDL <MDL <MDL <MDL
251 250 49 99 144 114.7 90.2
130 1017
103.0 150
111.4 77.4 5 71.7 142 96.1 142
99.1 44.7 774
117.4 74.2 7X7 1207 903 65J <34 41.1 544 444 115.9 345911 3S307S 314730 343395 422I2S 404243
343202 325352 214147 247144 2353SI 112144 143230 201253
Tatal Aafenat af PPOS 0* <MDL <MDL
<MDL <MDL 16% 4454 2354 3354
0144 01147 00902 0130 01027 01030 0150 am i 00776 0135 00717 0142 00944 0162 0.0991 00447 0.0772 01174 00742 00727 0121 00903 0.0459 00435 0.0411 0.0545 00445 0116
364 343 347 343 . 422
/ 4M 343 325 2H 244 235 142 143 204
Avance PPOS a rt <MDL <MDL 15% 21%
OHSS 00944 0140
00094 0.0571 00734
377 240 191
RSD ~ Std-Dev.
<MDL <MDL 00304 0354
I7.S 0.0211 36.9 00350 201 00374
23.6 00212 m OOI33 500 00344
039 31.6 105 25.2 7.72 I4J
POAA Cale Cane,
a rt <MDL <MDL
<MDL <MDL
219 247 223 173 0.00 OOO OOO 000 0.00 OOO
OOO 0.00
OOO 000 000 0.00 000 000
000 0.00 000 000 000 000 000 000 OOO 000 0.00 000 0.00 000
000 000 000 000 000 000 000 000 000 000 000 000 000 000
Tetal Aanaat af POAA
> rt <MDL <MDL <MDL <MDL
73% 19% 75% 54%
<MDL <MDL <MDL <MDL <MDL <MDL <MDL <MDL <MDL <MOL <MDL <MDL <MDL <MDL
<MOL <MDL
<MDL <MDL MDL <MDL <MDL <MDL
<MDL <MDL <MDL <MDL <MDL <MDL <MDL <MDL <MDL
<MDL <MDL <MOL <MDL <MDL <MDL
<MDL <MDL <MDL <MDL <MDL
Average POAA V i <MDL <MDL tl% 44%
<MDL <MDL <MDL
<MDL <MDL <MDL
<UDL <MDL <MDL
Method Defection Limb (MDL): P F 0 S -X 5 * lX 5 ap feP O A A -tX S affe
J a r t . PPHS - 3 af/g. M513 - JOa r t . M61J - 3 a r t
N/A-N ot Applicable prosPOAA-
DafeVoillcd/Aaaly;
2/30. 03/109, 3/17/99,03/22/9. 05/209* LAC
000099. 000099 EAD
PPHS MSI3 C9F19COOM6I3-C11F23COO-
BACK TO MAIN
RSD StADcv.
<S4DL <MDL 0199
Cric. CMC V
WS-JUDL .<: K c<m d l .
,*<. IM . t o *
0254
s ? 14. ;y .
.`;IM ,;V a& cm it. S filK i
<MDL
w m m & i' . `"r '4 0 1 'V
<MDL
^ 1 4 .1 0 ,
, <MDL
s ii
OOO
<MDL <MDL
*`^ : oooiVh ^ o o o /i -V
<MDL
W S*m 0! ISS W iA e.V 'f
Sw | S # ,0 0 r - 'i < V . (t
<MDL <MDL <MDL
fW !. .o o M m & w itsn
. % ^ 000'k.iL ^ ' AO.ib.' fw y m w B Sftio.tyv'
rt <MDL <MDL <m d l <m d l 41% 45%
41% 45%
0140 00409 OH4
0321 1.19 <MDL 0291
0401 00163 0.0144 00115 000449
HR HR
HR HR HR HR HR NR NR HR HR
HR HR NR HR HR
NR HR HR HR NR HR HR HR HR HR HR NR HR HR
V i <MDL <MDL
53% 53%
RSD Bid. Dev.
<MDL MDL 0454 0454
0375 00145 000449
101 0377
11.9 000194
PDfWM MMV/M
HR NR HR HR HR NR
NR HR NR HR NR HR
PACT-M-2.0 Excd Vcrtiea 7.0
TOX-12(Mivcr.xJa
01099 3:12 PM
sudr
FWdaal W ih n c r n l t K - f
Manie
MattoMarMa*
Aaalyllcal gyipT n l tyn m Neifcer
D auofExnaenoa/Aaalyat Dalao tAaalyiia^Aaalyit
Dm TOn M M wM aalyil: SaaipU Data
RATUVER
C rw f Dm
MctborfWk Mcthodl Blk Matrix Blk . Matrix Nk q c - im h *
C retf 4
3aoafOfu*r
Crampi 30.0 mp\pfKf Recovuy 1* MDayg
Cm p4 JO O au ty riay Recovery 2 - 21 Dayi
C rrap f IM oftyH qr
PFHS
C noS lOOa^/kffeay Recovery 1-14 D a IO0 f/kf/riey Recovery 2-21 D a
j.o*tfkpftr
PPOS
G rafi 1.0 Mf/kf/day Recovery 1- 14 Da ).0fcU iy Recovery 2 - SS Dry*
Saapla ff
H20 Blk-1 H20B1H lUMrit Uvw Blk-1 RabMl Livo Btk-2
104F 106F H0P IlOP 14SP 14<P 147F I4P I49P 190F 151P I52F 1S3F
4P
I55P 157P
I3IP UPP
'
19P I70P I71P I73P 174P 175F
I
HA HA HA HA 0.9240 0.9240 09242 09242
02443 04113 04437 044 0.4097 0.641 0447 04341 0157* 07221 01019 O titi 09430 0.919
O ff 0M4 01107 04779 07719 04292 04732 04371 09394 07797 0.9471 0176 07937 0.442
I7IP I79P 1MP
1I1P
H1II2PP
IMF ItSP IMF ft7F
IMF IMF 1*1F
04423 09462 0.110 07104 09940 0.4312 01207 1.0370 01343 07S4I 04719 0.44X2 0.9ISO
Ifa tolD M u toU rtp iD t)!
UiMM i-- .i PAG______J-120 i______ _ _
2S-Day O rai Tox Slady by Daily Garage la Rata
TOX-IM,Ezpionbxy2t-DayOraiToxicityStadyY t DailyGavine ia Rau
T-7023, T-70M,T-70B , T-7032. T-UM
dal tirar
m an
S a tinto ri/bt
PACT-M-1J>k FACT-M-2.0
R-&parca VaM:Sae Anactacau
MadaliaaMlOM,AaKia0634M,aadSop020ir9 Stopc '
MaaaLynl.1
Y-Mampi;
a3w/w2uXw9/tt,,RmaWvniWiw//S9A>),.Hatvaimi/a,,aanmsiia
hovmee/d u Duaumm
PFHS C klcC w c.
afe . * <MDL
MDL ~ , <MDL ' - - <MDL;
m sm
M utM
*435 1041*4 94742 91913 114992 S9U1 114702 76152
393 169 22 374 274 31.
:n:.''oOMo0o0i''tlPVo*O00OJM
OOO u s ti
IIIIIili
Tauri A n u t
rrpH s
rt <MDL <MDL <MDL <MDL
OH
OH OH OH
NR HR NR NR HR HR HR NR HR NR NR NR NR NR
4. 10
94.7 91.9 117 19.1 119 76.2 0393 0149 042 00372 00272 0031
NR NR NR NR NR NR NR NR
NR NR
NR
NR NR NR
Avance PFHS fe MDL <MDL OH OH
NR NR NR
RSD
SU-Dot.
MDL MDL DfVti! 4D1V/W
NR NR NR
M513 Cale. Case.
mt/t <MDL MDL
<MDL
MDL 27 212 179 162
OOO OOO OOO 0.00 OOO
OOO
ooo ooo
0.00 0.00 0.00
ooo ooo
0.00
8c iSe88e8e888l
97.1 0263 00120
194 14.7
444 O li
19.7 0.00302
o0.o0o0
0.00
ooo ooo ooo
88888888
NR NR
ooo
0.00
NR NR
0.00 0.00
ooo
NR NR 0.00
N/A-NotApp&ibU
Tata! A m u t al MS13
rt <MDL
MDL <MDL MDL 92% 94% 60% 54%
<MDL <MDL <MDL
MDL <MDL
<MDL <MDL <MDL
<MDL <MDL MDL MDL <MDL <MDL
MDL
MDL MDL <MDL <MDL <MDL
MDL <MDL MDL
MDL <MDL <MDL
<MDL <MDL
MDL MDL MDL MDL <MDL MDL MDL MDL <MDL MDL <MDL
<MDL
MDL <MDL
BACK TO MAIN
Avene* MS afe MDL <MDL 3% 97%
MDL <MDL MDL
MDL <MDL
<MDL
MDL <MDL
RSD Std. Dar. MDL MDL 00205
0103
MDL MDL <MDL
<MDL <MDL MDL
MDL MDL
<MDL
M 6 Cale. CMc.
afe <MDL <MDL <MDL <MDL
267 314 272 241 OOO OOO OOO OOO OOO OOO
ooo ooo ooo ooo ooo ooo ooo ooo ooo ooo ooo ooo ooo ooo ooo ooo ooo ooo ooo ooo ooo
Tata! Awaaat TM
afe <MDL <MDL <MDL <MDL 19% 105% 91% 11%
<MDL <MDL <MDL <MDL <MDL . <MDL <MDL <MDL <UDL <MDL <MDL <MDL <MDL
<MDL <MDL
MDL *MDL
MDL MDL MDL MDL MDL MDL MDL MDL MDL
n
ooo ooo ooo oooooo oooooo ooo ooo ooo 0.00 ooo ooo ooo
MDL MDL MDL MDL MDL MDL MDL MDL MDL MDL MDL MDL MDL MDL
Avertei MU MDL MDL 97%
MDL MDL MDL
MDL MDL
MDL
MDL MDL
RSD
. Dot.
MDL MDL 0141 0123
MDL MDL MDL
MDL
MDL MDL
Date Eetaad/Aaalyat: Date Vcrfleri/Aaalt:
O W N , OVIM), 03/ 17/ , 03/22/9, OS/2 06/K /99, 06/09A9 EAD
li-
mo-foni
MS13 C9FI9000*
M6I3 CI fF23COO-
FACT-M-2.0 Excel Vcnioa 7.0
T0X*l20*ttvrjJi
tnwn 3;12fM
---- - FAU-w-*wC-l20 I
2S-Day O ral Toz Study by Dally G avagt la Rata
Stady: hoAHhata(Te< Satomca):
Mabod/Revtnaa: Analytical E q w p M S ^ X i a k r
Dm* orExtnctfaarAadyec DMoTAnalysU/Aaatyal: Date of DaU Redoctioa/Aaaly*:
Sample Data
TOX*UOlExplontory2S-OayOralToskfeyStadybyDailyOsvajab Rati
T-7023,T-7024,T-7022,T-7032,T-6293
RatLiver
FDesame:
FACT-M-I.OA FACT-M-1*
R-5qacdVaia:
Madefiac041091,Soap020199
Slope
MamLyuU
Y-bwccpC
04/19*9 RWW/3CWSAH
04/10/99,05/1499,05/25*9 H01/DRBT
o v u m , a s rts m , o s n tm , o s n t m kjh/m ee/d rb/h q i
See AlUdmeats
RAT LIVER Craap Dow
Method BUc 0
Matrix Blk 0
QC-2M R*
qc-iM n *
QC-250 ppb
Cnap 1 0.0 mg/kg/ity
Cottavi
C raapl 0.0 mp/kg/day Recovery 1 - 14 Deyr
G reapl OOa^/kg/day Recovery 2 -21 Dtyi
G reapl 3A0avAUay Tekmer Alcohol
Group! 30.0 mg/kg/dxy Recovery 1 -14 Day
Groopl 30.0 mg/kf/diy Recovery 2 - 21 Day
Graap3 30.0 mf/kfUay
Tdomcr AcryiM
Croap]
,
30.0 mg/kg/day
Recovay l - 14 Deyr
GraapS
30.0mg/kg/dxy
Recovery 2-2 1 Doys
Sample i
H20B&-1 H20 Blk-2 b S E D w lS T Rabbit liver Btt-2
3M-MS 3M-MSD 6M-MS 6M-MSD I3M-MS 13M-MSD
IM 2M 3M 4M 5M 6M 7M IM 9M I0M DM 13M MM ISM 17M tIM 19M 20M 21M 22M 23M 24M 25M 26M 27M 29M 30M 3IM 33M 34M 35M 30M J7M JIM 39M 40M 41M 42M 43M 4SM 46M 47M
Extracted Wf.
E
0.00 0.00 0.00 0.00
0.9162 0.9162 0.9090 0.9090 1.0701 1.0701 oasi* 0.6999 0.9162 0.6126 0.1920 0.9091 0.0965 0.0267 0.6344 0.6972 0.0504 1.0701 0.9044 0.0511
1.1*03 0.9991 0l5039 Ol533I 07957 1.0663 0.7240 0.5990
05611 07356 09176 09513 . 1.0055 0.9514
06409 06715 05000 09225 05915 0.5744 07240 06670 1.0153 0.7J4J 0.9065 1.0667 09633 097I
pros Calc. C ent
a rt <MDL <MDL <MDL <MDL
274 206 319 300 209 291
170 129 102 124 104 130 127 165 115 339 312 94J 107 95.7 543
402 39.7 36.6 34.6 2SJ 19.2 29.0 32.9 26.0 25.5 44.9 93.0 31.7
70.4 30J 65.2 50.0 76.2 40.7 006 92.5 41.4
64.S 49.9 59.7 53.1 . 56.6
TatalAai--at afPFOS
A <MDL <MDL <MDL <MDL 92H 96% 107% 103% 97% 97%
0170 0129 0102 0124 0104 0131 0127 0.165 0115 0339 0.3119 0094 0107 0096
00545 00402 0.0397 00366 00346 00259 00192 0.0290 0.0329 00260 00255 00449 00930 0.0317
00509 . 00652 /o o sto
00762 00417 00006 0.0925 0.0414
00645 00499 00597 00531 00566
Avene* pros a rt <MDL MDL 94% 105% 97%
0133 0255 0099
0.0950 00201 00560
00670 0.0SI9 00565
MethodDdectioaLink(MDL): nOS-UatfePOAA-Sapfe PFHS-05ag/g,M513-25ead50art-U613-25>rt
DM Ealend/Aaaly*: D M V otfcd/A aaty:
0JA/99,05/26/99 LAC O0PC/99,06/0*99 BAD
USD 8td.Dc*.
<MDt <MDL
4% 4% 0.0
20 003 47. 01220 097 0.0069
306 0.0107
14.0 0.00416
57.6 0.03272
22.3 001515
ns 0.0117 5.10 000327
* POAA Cak. Cmk.
a rt <MDL <MDL tDiVAM 9D1VAM . 254
260 299.93 310.30 344.99 312.71 OOO 0.00 0.00 OOO 0.00 0.00 OOO 0.00 0.00 000 0.00 OOO OOO 0.00
5077 3415 1366 7W0 4944 7045 5713 10045 4197 3712 2173 1519 1662 2026 6005
1032S 1091 1559 1463 10095 7591 10021 4711
1545 3764 1157 117 1731
Tarai Amaaat CPOAA
a rt <MDL <MDL D1V/M DIVAN 5% 7% 100% 104% ' 115% 109%
<MDL <MDL <MDL <MDL . <MDL <MDL <MDL <MDL <MDL <MDL <MDL <MDL <MOL <MDL
5.01 3.49 037 73* 4.94 7.04 5.71 10.0 4J0 3.71 0*7 \S9 1.66 2.03
6.01 I0J 009 056 046 ion 7.59 10.0 4.7* US 3.76 1.16 O tt7 1.74
WA-Not Applicable
.FF0S-PcrflnoraoctaacaBUbaM POAA" FeriiaorooctasoM FFHS PcrfliorohcxaaemlfcnM MS13 * C9F19COOM 6I3Cl1F23CDO*
Average POAA a rt <MDL DIVAN MH 102% 110%
DIVAN DIVAN fDtVAH
639 339 IM .
064 3M IM
BACK TO MAIN
USD Sid. P ot.
<MDL DIVAN
2% 3% 010
DIVAN
DIVAN
DIVAN
317 2.1 10 0.669 103 0234
rc.i 14 402 MS 34J 0435
CMc. Case.
............ r t ______ NR NR NR NR
VALUE! VALUEt VALUE! VALUE! VALUE! VALUE! VALUE! VALUE! VALUE! VALUE! VALUE! VALUE! VALUE!
VALUE! VALUE! VALUE!
VALUE! VALUE! VALUE! VALUE!
VALUE! VALUE! VALUE! VALUE! VALUE! VALUE! . VALUE!
VALUE! VALUE! VALUE! VALUE! VALUE! VALUE! VALUE! VALUE! VALUE! VALUE! VALUE! VALUE! VALUE! VALUE! ' VALUE! VALUE! VALUEI VALUE! VALUE! VALUE! VALUE!
Tarai Amaaat af
* NR NR NR NR NR NR NR NR NR NR
NR NR NR NR NR NR NR NR NR NR
NR NR NR NR
NR NR NR NR NR NR NR NR NR NR NR NR NR NR
NR NR NR NR NR NR NR NR
NR NR
NR NR NR NR
Average a rt NR NR NR NR NR
NR NR NR
NR NR NR
NR NR NR
USD Sid. Dev.
NR NR NR NR NR
NR NR NR
NR NR NR
NR NR NR
FACT-M-2 0 Excel Venfoa 7.0
TOX-I20-livcr.xle
tnom 5:12 PM
FAC-- -- --10 21-Day O ral Tox Study by Dally Gavage In R ati
BACK TO MAIN
Pndaet M-- twT Mhatatce);
McAod/RcvUea: Aaalytkl E oslpnat MytUm H m k m hU nw ti S o ftw afw V ertio DattofExtnctW Auly: DU* o f Aaalyrit/AtiJyat: Dale of Dati Kidoctio^A--lyiC
S ia p U Data
TOX-120, Exploratory 2S-Dey Oral Toxidty Stady by DailyGavaga la Rats
T-7023, T-7GM, 7-7022, T-7032. T-4295
K Liver
SeeHattorighi
PCT-M-1.0APACT44-2.0 k tad d H 04109, l o # 000199
Mnd.yUl.2
R-Sqend VibcSceAttachments
--SN--C
W IV 9 9 RWW/X7/SAH
04/2A99,03/1499,05/23/99 HOJ/DRBT
04/23/99,03/03/99,03/1199,05/2699 KJH/MEE/DRR/HOJ
RAT LIVER ClM f Daaa
Method B& 0
Matrix Bft 0
Q C -2 5 0 B *
QC-U On*
Q C -JM p1*
C napl AOng/kffiday
C iani
Croopl 0.9 Agfiay Rocovwy t 14 D m
Craof 1 AOo#kgMay Recovery 2 *21 Dtye
Cmp 2 SAOng/kg/day Telarne* Alcohol
Craap2 SAOngftfttay Rocovoy 1-14 D m
Craapl M-Ong/koUay Racoray 2 *21 Dayi
Craap J SAOng/kgMay T d n u r AoyiiM
Groof 3 3A0 m t/kjK f Recovery 1 - 14 D m
Gnop J SAOng/tytey Recom y22ID m
-- ploi
tilrtdaiW L
H20BIM H20 BJk-2 RiMfl Liver Blk-1 . Rahbit Uvtr Bft-2
3M-MS 3M-MSD 6M-MS 6M-MSD 13M44S 13M-MSD
IM 2M 3M 4M 5M CM 7M fM 9M 10M 11M 13M (4M 15M
17M ISM I9M 20M
2IM 22M 23M . 24M 23M 2CM 27M 29M 30M 3IM
33M 34M 33M 36M 37M 3IM 39M 4QM 41M 42M 43M 43M 46M 47M
0.00 0.00 0.00 0.00 0.9102 0.9102 0.9091 0.9091 1.0701 1.0701
0.451 S 0.4999 0.9142 a 6124 0.1920 0.909 0.1945 0.1247 0.4344 0.4972 0.1504 1.0701 0.9044 0.1511 .
0.9993 0.5139 0.5331 0.7957 1.0443 0.724 0.5990 0.541 0.7354 09174 A9S13 1.0435 0.9514
0.4419 0.47(5 0JII0 0.9225 0391) 0.5744 0i7240 0.4470 1.0153 0.7343 0.9065 1.0647 0.9633 0.9711
rn is Cale. Case,
aa'c /MDL <MDL MDL MDL
273 254 319 311 247 291
49.0 94.4 49.7 50.9
SU 54.7 40lS 41.9 444 221 122 IM 90.4 419
34.4 454 SM 112.1 214 214 324 29* 34.9 14.9 22. 344 44.1 21.1 444 53.1 ai . 454 / 46.7 564 50.7 104 22.7 334 31.1 294 30.4 274
Method Detection U n * (MOL* P K - 2 J a * j . P O A A - 3 n f f c m t t
TatalAnaaat rrn u
_ gfr <MDL <MDL MDL MDL 91% 9% 107% 104% 9% 97% 0.0690 0.0966 0.0497 0.0509 0i0313 0.0547 0.040 O M I, 0.0442 0.221 a 122 0.10* 0.0904 0.0619.
0.0364 0.0455 0105S4
Ol 12 0.0254 0.0214 0.0320 0.0299 0.0349 A0I49 0.0221 0.0345 0.0441 0.0211 0.0643 0.0531 0.0901 0.0453 0.0667 0.0563 0.0507 0.1031 0.0227 010334 0.0311 0.029 0.0306 0.027
IMeEMcmd/Aaalyit: Data Verified'AaafyvC
05/05*9. 05/26/99 LAC 06W 99. 049999 BAD
|i i
MDL <MDL U% 105% 93%
0.0544 0.129 0.0190
0.0440 0.0255 0.0332
ao<43 0.0291 0.0293
R5D StADev.
<MDL <MDL
7% 2% 0.0
374 0.0204 a . 0.0M4
21. 0.0194
424 0.0211 404 0.0103 34J 0.0114
JA9 0.0205
194 0.00559
5.42 0.00159
U iii CUc Cm c .
Mt <MDL MDL <MDL <MDL
225 227 239.95 29340 217.43 267.66
aoo
0.00 0.00 ooo 0.00 0.00 0.00 0.00 0.00 A00 A00 A00 AOO 0.00
. 102 415 1615 2252 3155 2007 202!
2720 1491 2244 1443 1144 1222 1475
2304 2001 1727 1775 ISS) 1234 1619 1621 71 1174 1270 692 969 741
Total Am ont TM5I3
tfl MDL <MDL MDL <MDL 75% 76% 0% 9t% 73% 90%
<MDL <MDL <MDL <MDL MDL <MDL <MDL <MDL <MDL <MDL <MDC
<MDL MDL <MDL
1.03 A41 1.4 245 3.14 LOI L02
L72 1.49 244 144 144 142 1.47 240 2.00 1.73 1.77 1.SS 144 1.49 1.63 A79 1.17 147 A692 A949 A741
Avenge M913 rt <MDL <MDL 70% 9% 1%
<MDL <MDL <MDL
1J1 1.73 142
1.71 l.ot A01
ftSS RAD*.
MDL MDL
1% 20% A21
. <MDL
MDL
<MDL
44.0 AI7f 24.4 0.441 214 0423
174 407 23.7 0455 114 Al4i
N/A - Not Apptieabla 9FOS90A A - Perfhorooctanoolo PFHS-9 M3U-C9P19COOM 6I3- CI1923000.
M4U CalcCooc.
tft MDL <MDL
<MDL <MDL
241
229 117.04 21L60
225J1 233.71
AOO
AOO A00 A00 A00
.
AOO AOO AOO AOO AOO AOO AOO AOO AOO
9A4
494 ' 3.4 74.7
994
7L4
. 910 112 4L4 119 142 112 14 11 AOO AOO 424 AOO
AOO
AOO AOO AOO
AOO AOO 0.00 AOO
AOO AOO
Tetti A tm at TM4U
A <MDL MDL <MDL <MDL 11% 77% 43% 71% 76% 7%
MDL MDL MDL <MDL <MDL MDL <MDL <MDL MDL <MDL <MDL MOL <MDL <MDL 0.0906 0.0693 A0S34 A0747 10995
A0724 A0960
A lt* A0614 Al 19 0.142 A113 A0464 A il
MDL MDL A0622 MDL MDL MDL MDL MDL MDL MDL MDL MDL MDL MDL
Average M41J A
MDL
MDL
79%
47%
77%
RD StADev.
MDL MDL
9% 12% 0.03
MDL MDL MDL
MDL MDL MDL
Aom A107 0.106
174 A0150
3J A04I4
144 A0172
A0632 MDL MDL
N/A MDL MDL
FCT-W-2.0 EiedVfw*7.0
T0X -1204W xk
ifl/99 *12PM
FAfcrr-rrra-120 1 28-Day O ral Tox Study by Dally Gavage In Rats
BACK TO MAIN
PwdeetNH*o(Tal Sebetnce): Muric Method/RcvUem Attlytfcal Eqwpaoi SystemNeater:
Dm*orentacdoaMaabsl: Date ef Aaalynci'Aaatyit: DateofDau fedrtioaMaifyC
Sample Data
70X420 Exploratory2f*DeyOnt TmxkkySlaty byDeflyQ m* faRett T-7OE3..T-7024, T-7Q22, T-7002T-629S Rat Liver FACT-M4.0ft FACT-M-2.0 Madeline04109S '
MamLyn3.2 04/19199 RWW/JCMAH 04/20*9,05/21/99. 05/25/99 HOI/DRB/KJH7
OSA2S/9R05/14/99, OS/2/99 MEE/KJH/HJ
RAT LIVER CrM f Dm
MctbodBtk Matrix Blk
QC-JW ffk
Q C -Jn* O C -!*
C i |4 y
Creep 4 0 -V ^T Recovery 1 14 Days
Creep 4
Recovery 2-21 Dayt C rapf
1Q.0b | / | % PPHS
Groo^S lOOmfgtfay Recovery 1-14 D m
GreopS lOOaif/tfMay Recovery 2 - 21 Days
Creeps
pros
Cnr l.SmkpMqr Recovery 1-1 4 Day*
Creap 4 Recovery2*21 Days
hqhl
K20B&1 K20 BUt-2 Rabbit Liver Blk-1 Rabbit U r n Blk*2
3MMS 3M-MSD SM-MS 6M-MSD 13M-MS 13M-MSD
49M 50M SIM S2M S3M S4M SSM SAI S7M SSM S9M 4IM <2M 63M
SSM SSM S7M SIM 69M TOM 71M 72M
73M 74M 7SM 77M 7IM 79M
SIM S2M S3M S4M ISM MM 7M SSM STM
90M 9IM 93M 94M fSM
pros Ceec.
tfl 0 0 0
1 374.4500 314.0600 400.5000 3904100 379.4300 3S1.7SOO
NR NR NR NR NR NR NR NR NR NR NR NR NR NR
21.7
73.0 NR NR NR NR NR NR
154 96.3
90.3 117 SS.4 S3J
246.9000 500.2700 3411500
429.1100 412.0600 419.7200
455.7600 403.1500 3219700 3304600
261.3200 209.1100 254.0100 251.4300
MethodDette* Urn*(MDI* FfOS-3ftJiffcFOAA-2$*fe,
Ml
1.
1
1 1
1 1 1 1
1 1
1 1 1
1 1 I 1 1
1 1 1 ! 1 !
1 1 t 1 1 t 1 1 1 1 1
1 1 ! 1000 tooo 1000
1000
1000 . 1000
1000
(000 1000 tooo 1000 1000
1000
1000
pros Cak. Ceec
tfl <MDL <MDL <MDL <MDL 27409% 21573% unni 30793%
21164% 29091%
fVAL17EI VALUE! VALUE! VALUE! VALUE! VALUE! VALUE! VALUEI VAUJE1 VALUE! VALUE! VALUE! VALUE! VALUE!
21.5 7JJ VALUE!
VALUE! VALUEI VALUE! VALUE! VALUE!
153 %! 90.6
117 S9.6
4.0
245S20 50271
. 352317 / ' 433347
47*539 4*549
456739 400571
J15IM.1 332S25 2700211
79017 253062.1
250517.1
Date EaUroVAealyat Date Vcrified/Aufyit:
02/26/99,03/10/99, 03/17/99,03/72m . 0*24/99,05/26*9 LAC w a rn , w w n bad
Avrai pros <MDL <MDL 94% 105% 97%
NR NR NR
0050 0.114 6.1051
419.4 309J 1312
USD M.DCV.
<MDL <MDL
4% 4% 00
0.0 NR ao NR 0.0 NR
S2 0.0317 303 0.0344 24.1 0.0261
20.44 15.73 1I.M 34.19 10.7 25.4
POAA Cale. Ceec.
rato <MDL <MDL <MDL <MDL
254 260 299.93 310.30 344.99 312.71
VALUEI VALUE! VALUE! VALUEI VALUE! VALUE! VALUE! VALUE! VALUE!
VALUE! VALUE! VALUE! VALUE! VALUE!
0.00 0.00
VALUE!
VALUE! VALUE! VALUE! VALUE! VALUE!
0.00 0.00
VALUE! 0.00 0.00 0.00
VALUE! VALUE! VALUEI VALUE! VALUEI VALUE! VALUE!
VALUE! VALUEI VALUEI VALUEI
VALUEI VALUE! VALUE!
Tetti A a u e P ef POAA
er <MDL <MDL <MDL <MDL 15% 17% 100% 104% 115% 105%
NR NR NR
NR NR NR NR NR NR NR NR NR NR NR
0.00 aoo
NR
NR NR NR NR NR aoo aoo NR aoo 0.M aoo
NR NR NR
NR NR NR NR
NR NR
NR NR NR NR NR
N/Aa NotApplicable FFOSPOAA-P
HPHSPeritai
Avente POAA rato <MDL <MDL 16% 102% 110%
NR NR NR
ao aoo aoo
NR NR NR
BSD SM. Dee.
MDL MDL 0.0232 0.0340 ' 6-09*2
aoo NR aoo NR aoo NR
HV/M aoo
MV/M aoo
SUV/M aoo
aoo NR aoo NR aoo NR
Crie. Ceec. rato
VALUE! VALUE! VALUE! VALUE! VALUEI VALUE! VALUE! VALUE! VALUE! VALUE!
*07 2433 2447 2703 *03 2034 4347 5162 16S
29.4 It! 25.0
405 103
VALUE! VALUE!
VALUEI VALUE! VALUE! VALUE! VALUE! VALUE!
VALUE! VALUE!
VALUE! VALUE! VALUE! VALUE!
VALUE! VALUEI VALUEI VALUEI VALUE!
VALUE! VALUE! VALUE! VALUE! VALUEI VALUE! VALUE! VALUE!
VALUE!
rt NR NR NR NR NR NR NR NR NR NR 4.0 2.41 145 170 AIO) 101 4.35 5.SS ai6* 00294 OMI 00250 00405 00103
NR NR NR NR NR NR NR NR
NR NR NR NR NR NR
NR NR NR NR NR NR NR NR NR
NR NR NR NR NR
Avente rato NR NR NR NR NR
RSD ltd . Dee.
NR NR NR NR NR
1.09 0126 00253
SU US
` 66.6 O0S4O
59.7 00151
0 NR NR
aoo NR NR
aoo NR NR
aoo NR NR
aoo NR NR
aoo NR NR
FPtArCrTl-VMe-n2t.o0*7.0
TOX*120-Uvtrjdi
mom 5:12PM
FACT-75X-120 '
' *"
28-Day O ral Tox Study by Daily C iv a ie la Rate
BACK TO MAIN
Stady: frodati H a M T o t Sabataace):
Mctbod/Rcvirioa:
Aaalytfcal Eqriproc* Syrien N n h c r
lanrarocai SoftwarriVcrrioa:
D M ofE xlnctio^A ailytt
.
Date of AiulysiriAaalyri: Data of Dota RcdactioriAaaly:
S ta p l* Data
T0X'I2A BxplontfOX-13A Explantoiy 2S-Day Otri Toxkfcy Stady ly Daily Gavage la RM
'T-7Q23, T-7024, T-7D-7023, T-7024, T-7022, T-7032. T-629S
.
RatUvcr
RatUvcr
Stallai le righi
FACT-M*1.0 A FtPACT-M-l.O A FACT-M-10 . K-Sqund ValaeSce AtuduneMs
Maddfac 04109 Maddfae 041090
ManLynS.2 M rod.yn3J
OVIW9 RWW0OM/1W9 AWW/7CMAH
04/3<m, 03/31/99, 0V299, 03/21/99,03/25/99 HOJ/DRB/KJH7
0V0V99,osa*m, osmn. osa*m, osaan mee/kjh/ho;
RATUVER Craap
MohodBlk
Matrix Dlk 4
Q C -M O fi*
Q C -U O jfk
QC- 250ppb
Creay 4 30.0atyday
Grwty 4 30.0 m f t y i a y Recovcnr 1-14 D m
Craap4 30.0W V d a y Recovery 2-2 Deys
C rw yl 10.0ng/kfUay
PPHS
GreapS I 0 .0 m $ /k f/itoy Recovery 1-14 D an
GraapS 10.0 r o n d a r R teo m y l-llb m
Craap6 3.0 rog/fcf/day
PPM
Craap< S.Oafftfttay Raconuf 1-14 Dey*
Greap6 S.OmtfktfAay Recovery 2-2 1 Dey
S ta p k l
H20 Blk-1 H20Blk-2 Rabbtl LJvcrBtk-t Rabbif Uver Blk-2
3M-MS 3M-MSD tM-MS tM-MSD 13M-MS 13M-MSD
49M 30M 31M . 92M 33M 54M 33M 56M 37M 3IM 39M 6IM 62M 63M 65M 66M 67M 6M <9M 70M 71M 72M 73M 74M 7SM 7714 7SM 79M IM 2M ISM 4M ISM 4M 7M IM 9M . 90M 91M 93M 94M 95M
PFOS C ru. mtft
0 0 0 191 374.4500 314.0600 40QJOOQ 390.4400 379.4300 3 1.7300
NR NR NR NR NR NR NR NR NR NR NR NR NR NR 21.7 73. NR NR NR NR NR NR 154 913 90.3
117 14 13.3
2419000 30012700 3411500 429.1S00 4110600 419.7200 435.7600 403.3500
3219700 330.4600 2613200 209.1100 254.0100 251.4300
PPHS
CafeCeac.
rt <MDL <MDL <MOL <MDL
271 254
319 311 267.42 290J1
rVALUEl VALUS VALUS VALUS VALUS VALUS VALUS VALUS VALUS VALUS VALUS VALUS
VALUS VALUS
xom 339107 213354
337636 34SB1 316441 223309 317000 69362 41369 67999
17903 ,, 42766
2(199
VALUS VALUS VALUS VALUS VALUS VALUS VALUS VALUS VALUS VALU VALUS VALUS VALUS VALUS
M e tr i D rotdaa m (M M PPM - S A 3 */$. fOAA - 25 g. f
TaCalAaroaat
afPFHS ata <MDL <MDL <MDL <MDL 91% 5% 107% 104% 9% 97%
NR NR NR NR NR NR
NR NR NR
NR NR NR NR NR
360 359
213 331 346 316 223 317 69.4 414 61.0 37J 411 212
NR NR NR NR NR NR NR NX NR HR NR NR NR NR
Average PPHS irt <MDL <MDL 11% 103% 93%
NR NR NR
327 61.9 36J
NR NR NR
D M bttnd/A riy DateVarifledfAaalyri:
0U H *, 0S/1A99,03/17/99, 03/22/99,09 06W 99,06/09/99 EAD
USD SU-Dev. <MDL <MDL 0.0723 0.0237 0.0030
NR NR NR
13. 310 19.0 11.7 20.4 7.42
NR NR NR
M313 Cale. Cmk.
a rt <MDL <MDL <MDL <MDL
225 227 273.16 334.12 217.13 267.66
VALUS VALUS VALUE! VALUS VALUE! VALUS
VALUS VALUS VALUE! VALUS VALUE! VALUS VALUS VALUE!
VALUS VALUS VALUS VALUS VALUS VALUS
VALUS VALUS VALUS VALUS VALUS
VALUS VALUS VALUE!
VALUE! VALUS VALUS VALUS VALUS VALUS VALUS VALUS VALUS VALUS VALUS VALUS VALUS VALUS
TaUl Aaraaal
ef MS11
Pi <MDL . <MDL <MDL <MDL 75% 7H 91% 112% 73% 90%
NR NR NR NR NR NR NR NR NR NR NR NR NR NR
NR NR NR NR NR NR NR NR NR NR NR NR NR NR *
NR NR NR NR NR NR NR NR NR NR NR NR NR NR
Average MS13
__ * <MDL <MDL 70% 102% 11%
NR NR NR
NR NR NR
NR NR NR
N/A-NotAppeibla
PFOS-
OAA - rcrffcMieoctaaorie
PFHS-
BSD S U Dev; <MDC <MDL
1% 20% 011
NR NR NR
NR NR NR
NR NR NR
M613 Cak. Caac.
Pi <MDL <MDL
<MDL <MDL
341 229 117 213 225.91 233.71
VALUS VALUE! VALUS VALUS VALUS VALUS VALUE! VALUS VALUS VALUS VALUS VALUS VALUS VALUE!
VALUS VALUS
VALUE! VALUS VALUS VALUS
VALUS VALUS VALUS VALUS VALUS VALUS VALUS VALUE!
VALUS VALUS VALUS VALUS VALUS VALUS VALUS VALUS W alu i VALUS VALUS VALUS
VALUS VALUS
Tetri A m aat
' efM ltS a rt <MDL <MDL <MDL <MDL 1% 77% 63% 71% 76% 71%
NR NR
NR ' NR
NR NR NR NR NR NR NR NR NR NR
' NR NR NR NR NR NR NR
NR NR NR NR NR NR NR
NR NR NR NR NR NR NR NR NR NR NR NR
NR NR
Arrrage M613 rii <MDL <MDL 79% 67% 77%
NR NR NR
NR NR NR
NR NR NR
RSD StLDcv. MOL <MDL
9% 13% 0.03
NR NR NR
NR NR NR
NR NR NR
FACT-M-2.0 Excel Vcnloa 7.0
TOX-120-tiverjs
tnvn 3:12 PM
3M Environmental Lab - Fluorine Analytical Chemistry Team
BACK TO MAIN
PFS's
PFHS
perfluorohexanesulfonate
C6F13S03-
PFOS
perfluorooctanesulfonate
C8F17S03-
PFOSA
C8F17S02NH2
PFOSAA
C8F17S02N ((CH2CH 3)(CH 2C00)>
M 556
. C 8F17S02N ((H )(C H 2C 00))-
M 570
C8F17S02N ((CH3)(CH 2C00))-
PFO SEA perfluorooctanesulfonylethyl amide C8F17S02N ((CH2CH 3)(H))
. Et-FO SE-O H
C 8F17 S 0 2 N ((C H 2C H 3)(C H 2C H 20H ))
PFDS
perfluorodecanesulfonate
C 10F 21S O 3-**
PFCA's
POAA perfiuorooctanoate
M 513
perfiuorodecanoate
M 613 M463
perfiuorododecanoate
M563
M363
C7F15COOC9F19COOC11F23COOC8F17COO- ** C10F21COO- ** C6F13COO- **
PFS = perfluorm ated sulfoante PFCA = perfluorm ated carboxylic acid
** no standard m aterial available fo r confirmation;
3M Confidential - A ttom ey/Client W ork Product Privileged - D o N ot D isclose
BACK TO MAIN
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1
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11/09 '98 16:24 FAX 31736418543
N0T0X B.V.
BACK TO MAIN
uvo/Ui7
1.
1.
].
'
.
.
j.
PROTOCOL
1
T.
- "
: EXPLORATORY 28-DAY ORAL TOXICITY STUDY
' WITH
j
TELOMER ALCOHOL. TELOMER ACRYLATE,
PFHS AND PFOS (positive control)
'.
BY DAILY GAVAGE
| IN THE RAT
' FOLLOWED BY A 14/28-DAY RECOVERY PERIOD
J
NOTOX Project 242933 NOTOX Substances 63484, 83493, 83902, 84456 and 84141
3M Test No. T-7023. T-7024, T-7022. T-7032, T-6295 - page 1 of 16 -
11/09 '98 16:24 FAX 31736418543
1
______________
NOTOX B.V.
BACK TO MAIN
0004/017
1
TELOMER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PFOS
NOTOX Project 242933
3M Test No. T-7023, T-7024
1 T-7022, T-7032, T-6295
PROTOCOL APPROVAL 1
]
STUDY DIRECTOR : 1
]
I-
Ir. S.C.M. de Hoog Date: 11 . S Q p b Q r r y b 1 Q Q < 9
SPONSOR:
Marvin T. Case, DVM, PhD Corporate Scientist 3M Toxicology Services
. ^r
J
j
]
'
D*t,:
i?re
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11/09 '93 13:23 FAX 31736413343
NOTOX B.V.
1
i TELOMER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PFOS
BACK TO MAIN
0003/017
NOTOX Project 242933
] PREFACE
Sponsor
]
Study Monitor ]
} Invoice Contact -
1.
Testing Facility .
1 Study Director Pathology
3M Toxicology Services 3M Center, Bldg 220-2E-02 St. Paul. MN 55144-1000 USA Marvin T. Case, DVM, PhD 3M Toxicology Services 3M Center, Bldg 220-2E-02 Telephone (651)- 733-5180 Fax (651)- 733-1773 Barbara Nelson 3M Procurement Operations 3M Center, Bldg 224-1N-04 St. Paul, MN 55144-1000 Telephone (651)- 733-0766 Fax (651)- 733-1799 NOTOX B.V. Hambakenweterlng 3 5231 DD 's-Hertogenboseh The Netherlands Xr. S.C.M. de Hoog Dr. J. Th. Wilson
4
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11/09 *93 16:25 FAX 31736413543
1------------------------
N0T0X B.V.
TELOMER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PFOS
BACK TO MAIN
008/017
NOTOX Project 242933
STUDY PLAN
] Start of Treatment
06 October 1998 (males)
] 08 October 1998 (females)
Start Recovery
04 November 1998 (males) 1 06 November 1998 (females)
I Blood sampling 03 November 1998 (Main groups, males) 04 November 1998 (Main and Recovery groups 1 and 2, males) 05 November 1996 (Main groups, females) 06 November 1998 (Main and Recovery groups 1 and 2, females) 18 November 1998 (Recovery groups 1, males) 20 November 1998 (Recovery groups 1, females) 02 December 1998 (Recovery groups 2, males) 04 December 1998 (Recovery groups 2, females)
Urine collection
predose
29-30 September 1998 (Recovery groups 1 and 2)
day 7
11-12 October 1998 (Recovery groups 1 and 2, males)
day 7
13-14 October 1998 (Recovery groups 1 and 2, females)
J day 14 day 14 end of treatment
18-19 October 1998 (Recovery groups 1 and 2, males) 20-21 October 1998 (Recovery groups 1 and 2, females)
03-04 November 1998 (Recovery groups 1 and 2, males)
end of treatment 05-06 November 1998 (Recovery groups 1 and 2, females)
end of recovery 17-18 November 1998 (Recovery groups l, males)
end of recovery 19-20 November 1998 (Recovery groups 1, females)
]
end of recovery end of recovery
01-02 December 1998 (Recovery groups 2, males) 03-04 December 1998 (Recovery grdups 2, females)
Necropsy
] 03 November 1998 (Main groups, males)
04 November 1998 (Main groups, males) 05 November 1998 (Main groups, females) 06 November 1998 (Main groups, females) 18 November 1998 (Recovery groups 1, males) 20 November 1998 (Recovery groups 1, females) 02 December 1998 (Recovery groups 2, males) 04 December 1998 (Recovery groups 2, females)
Draft Report
J 26 March 1999
Page 4 -
11/09 '98 16:25 FAX 31736418543
l
NOTOX B.V.
BACK TO MAIN
0007/017
TELOMER ALCOHOL/TELOtCR ACRYLATE/ /PFHS/PFOS ].
NOTOX Project 242933
TEST SUBSTANCES
The sponsor 1s responsible for the completeness of all test substance data. All available Information on characteristics of the test substance will be taken Into account before start of the study 1n order to determine 1f they conflict w1th/m1ght affect the proceeding of the study.
NOTOX SUBSTANCE 83484
(data will be added by protocol amendment)
Identification Description Batch Purity Test substance storage Stability under storage conditions Expiry date Stability 1n vehicle Safety precautions Disposal Category
TELOMER ALCOHOL (T-7023)
Stable
1% aqueous carboxymethyl cellulose: not Indicated
Corn oil: not indicated
Gloves, goggles and face mask to ensure personnel
health and safety
NOTOX SUBSTANCE 83493
(data will be added by protocol amendment)
1 Identification Description
TELOMER ACRYLATE (T-7024)
Batch
J Purity Test substance storage Stability under storage
conditions
Stable
Expiry date
] Stability 1n vehicle
1% aqueous carboxymethyl cellulose: not indicated Corn oil: not Indicated -x
Safety precautions
Gloves, goggles and face mask to ensure personnel
health and safety
] Disposal Category
i - Page 5 -
11/09 -98 18:28 Fa x 31738418543
L _ _________
NOTOX B.V.
1 , TELOMER ALCOHOL/TELOMER ACRYLATE/' /PFHS/PfOS
BACK TO MAIN
@008/017
'
NOTOX Project 242933
NOTOX SUBSTANCE 83502 (data will be added by protocol amendment)
1 Identification Description
(T-7022)
Batch
Purity
Test substance storage
] Stability under storage conditions
Stable
Expiry date
Stability 1n vehicle
1% aqueous carboxymethyl cellulose: not indicated
1
Safety precautions Disposal Category
Corn oil: not Indicated Gloves, goggles and face mask to ensure personnel health and safety
NOTOX SUBSTANCE 84456 (data will be added by protocol amendment)
Identification
PFHS (T-7032)
Description
Batch
Purity
Test substance storage
Stability under storage
conditions
Stable
Expiry date
Stability 1n vehicle
' 1% aqueous carboxymethyl cellulose: not Indicated
J Safety precautions Disposal Category
Corn oil: not Indicated
A
Gloves, goggles and face mask to ensure personnel
health and safety
NOTOX SUBSTANCE 84141 (data will be added by protocol amendment)
] Identification
PFOS (T-6295)
Description
'
Batch
-
] Purity Test substance storage
Stability under storage
conditions
Stable
Expiry date
Stability In vehicle
1% aqueous carboxymethyl cellulose: not Indicated
Corn oil: not Indicated
Safety precautions
Gloves, goggles and face mask to ensure personnel
health and safety
Disposal Category
] Vehicle
1% aqueous carboxymethyl cellulose or Corn oil, specific gravity 0.92
Rationale for vehicle
Based on trial formulations performed at NOTOX
Stability 1n vehicle
Stability over 4 hours will be determined by the *
sponsor
- Page 6 -
11/09 '93 16:26 FAX 31736413543
NOTOX B.V.
BACK TO MAIN
009/017
TELOMER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PFOS . NOTOX Project 242933
TEST SUBSTANCE FORMULATION
Method Storage conditions
Formulations (w/w) will be prepared dally within 4 hours prior to dosing. If necessary, adjustment will be made for specific gravity of vehicle. At ambient temperature
No chemical analysis of dose preparations will be performed.
PURPOSE AND RATIONALE
The nature and purpose of this exploratory study will be to determine and compare the relative oral toxicity in rats following oral (gastric gavage) doses administered daily over 28 days and Identify developmental compounds of lesser toxicity for commercial development. This study should provide part of a rational basis for toxicological risk assessment 1n man. The oral route was selected as it Is a possible route of human exposure during manufacture, handling or use of the test substance.
GUIDELINES
This protocol was reviewed and agreed by the Article 14-functionary and the
Ethical Committee of NOTOX as required by the Dutch Act on Animal
Experimentation.
The study procedures described 1n this protocol will be based on the following
guidelines:
.
1) EC Directive 96/54/EEC, Annex IV.D, replacing EEC Directive 67/54B/EEC, Part
B : Methods for the Determination of Toxicity and other'Health Effects; B.7:
'Repeated Dose (28 days) Toxicity (oral)*. Official Journal of the European
Communities No. L248, September 1996.
'
2) OECD Guideline-for Testing of Chemicals, Guideline 407: 'Repeated Dose 28>day Oral Toxicity Study 1n Rodents*, Paris Cedex, 27 July 1995
This protocol, study procedures and the report will not be Inspected by the NOTOX Quality Assurance Unit.
ARCHIVING
NOTOX B.V.. will archive the following data for at least 10 years: protocol, raw data, report and all specimens. No data will be withdrawn without the sponsor's written consent.
- Page 7 -
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NOTOX B.V.
1 TELOMER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PFOS
]
BACK TO MAIN
0010/017
NOTOX Project 242933
TEST SYSTEM
] Test System ]
Sprague Dawley rat CrV.CD BR, outbred, SPF quality. Recognised by International guidelines as the recommended test system (e.g. EPA, FDA, OECD, EEC). Females will be nulllparous and non-pregnant. Source : Charles R1yer, Sulzfeld, Germany
Age at Start of Treatment Approximately 6 weeks
1 Number of animals
Randomisation
84 males. 84 females At least 5 days before study start, by computer generated random algorithm according to body weight, with all animals within 20% of the sex mean. A health Inspection will be performed prior to commencement of treatment to ensure that the animals are 1n a good state of health.
Identification
Earmark and tattoo
ALLOCATION
Group Identification
Test Substance
Number of animals Animal numbers
males females
males females
1 Main group 1 Recovery group i 1 Recovery group 2 2 Main group 2 Recovery group 1 2 Recovery group 2 3 Main group 3 Recovery group 1 3 Recovery.group 2 4 Main group 4 Recovery group 1 4 Recovery group 2 5 Main group 5 Recovery group 1 5 Recovery group 2 6 Main group 6 Recovery group 1 6 Recovery group 2
Vehicle control
Vehicle control
Vehicle control Telomer Alcohol Telomer Alcohol Telomer Alcohol Telomer Acrylate Telomer Acrylate Telomer Acrylate
PFHS PFHS. PFHS PFOS (positive control) PFOS (positive control) PFOS (positive control)
8 3 3 8 3 3 8 3 3 8 3 3 8 3 3 8 3 3
8 3 3 8 3 3 .8 3 3 8 3 3 8 3 3 8 3 3
1- 8 ! 97-104 9-11 105-107 13-15 109-111 17-24 113-120 25-27 121-123 29-31 125-127 33-40 129-136 41-43 137-139 45-47 141-143 49-56 145-152 37-59 153-155 61-63 157-139 65-72 161-168 73-73 169-171 77-79 173-175 81-88 177-184 89-91 185-187 93-95 189-191
i Test substances Telomer Alcohol (substance 83484), Telomer Acrylate (substance
j
83493) and
(substance 83502) to be tested at a dose level of 30 mg/kg body
J we1ght/day.
I PFHS (substance 84456) to be tested at a dose level of 10 mg/kg body weight/day. PFOS (substance 84141) to be tested at a dose level of 3 mg/kg body weight/day.
I - Page 8 -
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11/09 '98 18:27 FAX 31738413543 1
NOTOX B.V.
TELOMER ALCOHOL/TELOffiR ACRYLATE/ /PFHS/PFOS
BACK TO MAIN
0011/017
NOTOX Project 242933
ANIMAL HUSBANDRY
Conditions Air-conditioned room with approximately 15 air changes per hour and controlled environment with optimal conditions being a temperature of 21 3*C and a relative humidity of 30-70%. Lighting will be a 12 hours artificial fluorescent light and t 12 hours dark per day.
Accommodation
Group housing of 3-4 animals per sex per cage 1n stainless steel suspended cages
with wire mesh floors. Acclimatisation period Is at least 5 days before start of
treatment under laboratory conditions.
.
Diet
.
Free access to standard pelleted laboratory animal diet (from Carfll Quality
BVBA,.Oud-Turnhout, Belgium). Each batch will be analysed for nutrients and
contaminants are analysed on a regular basis. Results are examined and archived.
Water
Free access to tap-water. Certificates of analysis (performed quarterly) are
examined and archived.
'
TREATMENT
!
| Method
I Frequency
| Dose volume
| Dose level 1I *
j RECOVERY
Oral gavage, using a stainless steel stomach tube.
Formulations will be placed on a magnetic stirrer ' '
during dosing.
3
Once dally for at least 28 days, approximately the same time each day, 7 days per week. Main group animals are dosed up to the day prior to necropsy.
. 5 ml/kg body weight. Actual dose volumes will be
calculated weekly according to the latest body
weight.
..
Group 1: 0 mg/kg b.w./day (Vehicle Control)
Group 2: 30 mg/kg b.w./day (Telomer Alcohol)
Group 3: 30 mg/kg b.w./day (Telomer Acrylate)
Group 4: 30 mg/kg b.w./day
'
Group 5: 10 mg/kg b.w./day (PFHS)
Group 6: 3 mg/kg b.w./day (PFOS, positive control)
Duration
Recovery group l: at least 14 days Recovery group 2: at least 28 days
t \
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11/09 '93 1:2S FAX 31738418543
l
NOTOX B.V.
1 . TEIOMER ALCOHOUTELOMER ACRYLATE/ /PFHS/PFOS
BACK TO MAIN
0012/017
NOTOX Project 242933
OBSERVATIONS
1 Mortality / Viability
1
Clinical signs
1
Body Weights
]
Food consumption Water consumption
Twice dally. Animals showing pain, distress or discomfort, which 1s considered not transient in nature or is likely to become more severe, will be sacrificed for humane reasons. The time of death will be recorded as precisely as possible.
Twice dally, detailed clinical observations will be
made 1n all animals: once Immediately after dosing
and once approximately 4 hours after dosing. The
time of onset, degree and duration will be
recorded.
.'
All symptoms will be recorded and graded according
to fixed scales (specified in the report).
Treatment period : on days 1, 5. 8, 12, 15, 19, 22, 25 and 28. Recovery period : on days l, 5, 8, 12, 14, 19, 22, 25 and 28.
Weekly.
.
Subjective appraisal will be maintained during the study and a quantitative assessment Introduced 1n the event of a suspected treatment related effect.
CLINICAL LABORATORY INVESTIGATIONS (Main and Recovery group animals) ?
J Blood samples will be collected under light ether anaesthesia, between 7.30 and 9.30 a.m. Immediately prior to post mortem examination for Main and Recovery group animals, and for Recovery group animals also at the end of the treatment
1 period. The animals will be fasted overnight (with a maximum of 20 hours) before blood sampling, but water will be provided. Blood samples will be drawn from the retro-orbital sinus of all Main and Recovery rats/sex/group and collected Into
J tubes prepared with EDTA for haematologlcal parameters (0.6 ml), with citrate for clotting tests (1.0 ml) and untreated tubes for clinical biochemistry parameters (>2.0 ml).
i
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11/09 '98 16:28 F A X 31736418543
NOTOX B.V.
BACK TO MAIN
0013/017
i _______________________________________________- __________________________________:
I ' TELOMER ALCOHOL/TELOMER ACRYLATE/ /PFHS/PFOS
MOTOX Project 242933
! HAEMATOLOGY
The following haematology parameters will be determined from blood prepared with EDTA as an ant1-coagulant, using the Instruments listed:
Parameter
Unit
Instrumentation
J
Erythrocyte count
T/l
] Haemoglobin Haematocrlt
mmol/1 1/1
. Mean corpuscular 1 volume
fl
iJ 1!1 .
Mean corpuscular haemoglobin . Mean corpuscular haemoglobin concen tration Platelet count
fmol mmol/1
G/1
11
JI
Red cell distribution
width
%
Total leucocyte count G/1
Differential leucocyte l(rel)
count
Sysmex K-1000 Sysmex K-looo 'Sysmex K-1000 Sysmex K-1000.
Sysmex K-1000
Sysmex K-1000
. .
Sysmex K-1000
Sysmex K-1000 Sysmex K-1000
Manual (Microscope)
^
1
The following haematology parameters will be determined.from blood prepared with citrate as an ant1-coagulant, using the Instruments Hstfed:
Parameter ]
Unit
Instrumentation
Prothrombin time
sec
J
Partial thromboplastin
time
sec
Sysmex CA-5000 Sysmex CA-5000
.
- Page 11 -
1 1 / 0 9 ' 9 8 1 6 : 2 9 FAX 3 1 7 3 8 4 1 8 3 4 3
NOTOXB.V.
BACK TO MAIN
_____________ _____
1
______________ _____________________
-- ___________________________________
telomer alcohol/t e l o m -R ACRYLATE/ /PFHS/PFOS 1
NTOX Project 242933
CLINICAL BIOCHEMISTRY
The following clinical biochemistry parameters will be determined from serum samples using the Instruments listed:
Parameter
Unit
Instrumentation
J
Alanine am1notrans>
jikat/1
ferase
1 Aspartate amlnotrans* jikat/1 1 ferase
, Bilirubin, total pmol/1
1
Cholesterol, total
mmol/I
Triglycerides
j Creatinine
mmol/1 |imo1/1
Glucose
mmol/1
Urea .
mmol/1
Protein, total .
Q/1
j . Protein, albumin
g/1
Protein, globulin
g/1
J Albumin Globulin ratio l
Alkaline phosphatase (ikat/1
j Sodium
mmol/1
Potassium
' mmol/1
J Chloride
mmol/1
Calcium
mmol/1
| Phosphorus
mmol/1
Epos selective analyser 5060 (Eppendorf)
Epos selective analyser 5060 (Eppendorf)
Epos selective analyser 5060 (Eppendorf)
Epos selective analyser 5060 (Eppendorf)
Epos selective analyser 5060 (Eppendorf)
Epos selective analyser 5060 (Eppendorf)
Epos selective analyser 5060 (Eppendorf)
Epos selective analyser 5060 (Eppendorf)
Epos selective analyser 5060 (Eppendorf)
Epos selective analyser 5060 (Eppendorf)
Calculation (Protein total - Albumin)
Calculation [Albumln/Qlobul1n]
Epos selective analyser 5060 (Eppendorf)
EFIX
5055
Flame Photometer
,\
(Eppendorf).
EFIX 5055 Flame Photometer (Eppendorf)
Jenway PCLM3
EFIX 5055 Flame Photometer (Eppendorf)
Epos selective analyser 5060 (Eppendorf)
t - Page 12 -
11/09 '93 16:29 F1 01736413543
NOTO! B.V.
BACK TO MAIN
1015/017
TELOMER AlCOHOl/TELOMER ACRYLATE/ /PFHS/PFOS
NOTOX Project 242933
URINALYSIS (Recovery group animals only)
Urine samples will be collected overnight (approximately 16 hrs) from all animals assigned to the Recovery groups according to the following schedule:
- predose - during the treatment period on days 7, 14 and at the end of treatment at the end of the recovery period, prior to scheduled necropsy.
During the sampling period animals will be deprived of food but water will be
provided. Urine will be collected into a specimen vial, using a metabolism cage.
After collection the samples will be frozen and stored at -20*C at NOTOX until
further analysis.
. .
PATHOLOGY
NECROPSY
.
All animals surviving to the end of the observation period and all moribund animals will be deeply anaesthetised using ether vapour and subsequently exsanguinated. All animals will be necropsled and descriptions of all macroscopic abnormalities will be recorded.
Prior to exsangulnation a blood sample (1*2 ml) for serum collection will be
taken from the aorta. Serum samples will be stored at -20*C at NOTOX until
further analysis.
'
Samples of the following tissues and organs will be collected from alif animals
at necropsy and fixed 1n neutral phosphate buffered 4% formaldehyde solution:
Identification marks
Adrenal glands
Aorta
Brain
'
Caecum
Colon
Duodenum '
Epididymides
Eyes with optic nerve and Harder1an gland
Female mammary gland area
Heart
Ileum
Jejunum
Kidneys
Liver
Lung, Infused with formalin
Lvmoh nodes mandibular, mesenteric
Oesophagus
Ovaries
Pancreas
. "
'
- Page 13 -
11/09 '98 18:30 FAX 31738418343
1-- - - - - - - - - - - - - - -
NOTOX B.V.
BACK TO MAIN
0018/017
TELOMER AlCOHOL/TELOMER ACRYLATE/ /PfHS/PFOS
NOTOX Project 242933
II
Peyer'* patches (jejunum, ileum) if detectable
Pituitary gland
] Prostate gland Rectum Salivary glands - mandibular
Sciatic nerve
''
] Seminal vesicles Spinal cord -cervical, mldthoradc, lumbar . Spleen
Sternum with bone marrow
Stomach
]
Testes Thvmus
Thyroid Including parathyroid
Trachea
Urinary bladder
'
Uterus.
All gross lesions
ORGAN WEIGHTS
The following organ weights (and terminal body weight) will be recorded from the surviving animals on the scheduled day of necropsy:
Adrenal glands
j Brain Heart Kidneys Liver
J Ovaries Spleen Testes
.,,
. _
*
. H
.
J Both absolute and relative (% body weight) organ weight data will be Included 1n
the study report.
.
LIVER SAMPLING
] From all animals surviving until the scheduled necropsy at least 1 gram of the left lateral liver lobe will be frozen in isopentane/N*. Liver samples will be i stored at -20*C at NOTOX until further analysis.
I
I
1
Jj
- Page 14 -
11/09 '9 16:30 FAI 31736416543
NOTOX B.V.
1- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - _ _
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------------------ :
I j TELONER ALC0H0L/TE10MER ACRYLATE/ /PFHS/PFOS
NOTOX Project 242933
1.
J HISTOTECHNOLOGY
A ll organ and tissue samples, as defined under Histopathology (following), w ill
] be processed, embedded and cut at a thickness of 2-4 micrometers and stained with haeraatoxylin and eosln. Special stains w ill be used at the discretion of the pathologist.
HISTOPATHOLOGY
Slides of a ll underlined organs and tissues (see under Necropsy) collected at the scheduled sacrifice from a ll animals, as well as-from a ll animals of a ll dose groups which died spontaneously or were terminated in extremis, and a ll gross lesions of a ll animals (a ll dose groups) w ill be examined by a pathologist. A ll abnormalities w ill be described and included in the report.
I STATISTICAL ANALYSIS i -------------------------------
The following sta tis tica l methods w ill be used to analyse the data:
I f the variables can be assumed to follow a normal distribution, the Dunnetttest (many-to-one t-te s t) based on a pooled variance estimate w ill be applied fo r the comparison of the treated groups and the control groups for each sex.
The S teel-test (many-to-one rank test) w ill be applied Instead of the Dunnett test 1f the data can not be assumed to follow a normal distribution.
A ll tests w ill be two-sided and 1n a ll cases p < 0.05 w ill be accepted as the lowest level of significance.
Additional methods of statistical analysis may be used at the discretion of the Study Director. The methods and the results w ill be described in the report.
References:
- C.W. Dunnett A Multiple Comparison Procedure for Comparing Several Treatments with a Control, J. Araer. Stat. Assoc. 50, 1096-1121 (1955).
*
j - R.G. M iller Simultaneous S tatistical Inference, Springer Verlag, New York (1981).
i
BACK TO MAIN
1I
1
i Page 15 -
1
3 ----- :- - - - - - - - - - - - - - - - - ' - - - - - - - - - - - r 1
TELOMER ALCOHOL/TELOHER ACRYLATE/ /PFHS/PFOS
1
DISTRIBUTION
This protocol w ill be distributed as follows:
J Original: 1 Copy:
J 1 Copy: 1 Copy: 1 Copy:
1 Copy: 1 Copy:
Study Director Coordinating Blotechnldan Clinical Pathology Necrop$y/H1stotechn1que Hlstopathology Management
Sponsor
NOTOXProject 242933
'f
V'
STF HIS 325/03
BACK TO MAIN
N M otox
Safety & Environm ental Research B.V.
SPECIMEN DISPATCH LIST
NOTOX PROJECT: _242933_
SPECIES:
RAT
TEST SUBSTANCE:
83484, 83494, 83502, 84456, 84141
SIGNATURE/DATE STUDY DIRECTOR
8 dQn-0^ 1999
TO:
Name and adress company: 3 M Environmental Technology and Safety Services
Attn. Kris Hansen
935 Bush Avenue
Building 2-3E-09
.
USA Ptci'
tei-flS |H8o C k t m o a e o J es
te l. 001 6 3 1 7 3 S 1 8 0 C n o ru n T . C o.se)og
cov CPa-uX. L e x t e r )
Group 1 Group 2 Group 3 Group 4 Group 5 Group 6
Animal numbers
males
1-li +
13-15
17-27 + 33-43 + 49-59 + 65-75 + 81-91 + 29-31 45-47 61-63 77-79 93-95
Animal numbers
females
97-107 + 113-123+ 129-139+ 145-155+ 161-171+ 177-187 +
109-111 125-127 141-143 157-159 173-175 189-191
Number of liver
samples
28
28 28
28
28 *
28
Also enclosed are :
[ ] Copy of Protocol/Amendments [ ] Copies of histology raw data [ ] Study results
[ ] Necropsy data transfer file
C]
.
DISPATCH: Signature:
Date:.
O
...
PLEASE SIGN AND RETURN THIS FORM AS SOON AS POSSIBLE AFTER RECEIPT:
COMPANY/SIGNATURE. C V i.A . .CwH^.. . . ?/*l. Date:... ^ o ? l f . M .
BACK TO MAIN
LisaA. Clemen
^ 02/06/99 04:20 PM
Environmental Technology &Safety
Services
'
(651)778-5568
3M Building 02-3E-09 PO Box 3331 S t Paul, MN 55133-3331 Fax: (651) 778-6176
To:
cc:
Subject
Kris J. Hansen/US-Corporate/3M/US@3M-Corporate Karl J. Rambo/US-Corporate/3M/US@3M-Corporate Grant A. PaulAJS-Corporale/3IWUS@3M-Corporate
`
NOTOX 242933 Sample Receipt
.
-
On 02/03/99 we received 168 rat liversfromNOTOX 242933 (TOX120, W1220). These sampleswere
receivedfrozenondry iceand have beenstored inFreezer 9 at -20 +V10C.
*
Lisa SAMPLES
4 \/BRlFteC> G f a f a f a
.
S'
3M Environmental Technology and Safety Services
attn. Kris Hansen
935 Bush Avenue
Building 2-3E-09
.
St. Paul, MN 55133-3331
USA
BACK TO MAIN
H^NOTOX
Hambakenwetering 3 RO. Box 3476 5203 DL 's-Hertogenbosch The Netherlands Tel.:(073)641 95 75 Fax: (073) 641 85 43
Date : 29-0AN-99 Ref. ; KiM Subject: NOTOX Test Substance No. 83484, 83494, 83502, 84456, 84141
Dear Kris,
On request of Mrs. S. de Hoog herewith I send the Uver samples (total 168) from NOTOX project 242933.
With kind regards, NOTOX B.V.
Mariette de Klnkelder Test Substance Officer
enclosure: a.m. Liver samples
Chamber o f Commerce VHertogenboseh nr. 16055281
BACK TO MAIN
NOTOX Project #242933
Feb. 4,1999
The liver sample numbers listed below did not include a collection date on their containers. After reading the protocol, I wrote the collection date on each container. See below for dates. If these collection dates are not correct, please call me at 1-165-778-5568.
Regards, Lisa A. Clemen
Ac
Samples 1 2 3 4 17 18 19 20 33 34 35 36 49 50 51 52 65 66 67 68 81 82 83 84
Group 1 1 1 1 2 2 2 2 3 3 3 3 4
'4 4 4 5 5 5 5 6 6 6 6
Collection Date Added
? 11/03/98
711/03/98
711/03/98
.
711/03/98
7 11/03/98.
711/03/98
711/03/98
? 11/03/98
7 11/03/98
? 11/03/98
711/03/98
? 11/03/98
? 11/03/98
? 11/03/98
711/03/98
? 11/03/98
? 11/03/98
711/03/98
711/03/98
7 11/03/98
711/03/98
711/03/98
7 11/03/98
711/03/98
Samples 109 110 111 125 126 127 141 142 143 157 158 159 173 174 175 189 190 191
Group 1 1 1 2 2 2 3 3 3
.4 4
4 5 5 5 6 6 6
Collection Date Added ? 12/04/98 ? 12/04/98 ? 12/04/98 7 12/04/98 ' 7 12/04/98 7 12/04/98 . ? 12/04/98 7 12/04/98 ? 12/04/98 7 12/04/98 7 12/04/98 ? 12/04/98 ? 12/04/98 ? 12/04/98 ? 12/04/98 ? 12/04/98 ? 12/04/98 ? 12/04/98
NOTOX #242933
TOX120 Liver Samples Received 02/03/99
BACK TO MAIN
' Samples Group Collection Date
*1 1 11/03/98
'2 1 11/03/98
'3 1 11/03/98
' 4 1 11/03/98
'5 1 11/04/98
*-6 1
11/04/98
'7 1 11/04/98
'8 1 11/04/98
*9 1
11/18/98
'10 1 11/18/98
'l l 1 11/18/98
S3 1
12/02/98
'1 4 1
12/02/98
'1 5 1 12/02/98
<7 2
11/03/98
-18 2
11/03/98
'1 9 2 11/03/98
'20 2
11/03/98
'2 1 2
<22 2
<23 2
11/04/98 11/04/98 11/04/98
'2 4 2
11/04/98
'2 5 . 2
11/18/98
'2 6 2
<11 2 <2.9 2
11/18/98 11/18/98 12/02/98
<30 2
12/02/98
'3 1 2 12/02/98
'3 3 .3
11/03/98
"34 3
11/03/98
<35 3
11/03/98
-36 3
31 3
11/03/98 11/04/98
'3 8 3
<39 3
11/04/98 11/04/98
'4 0 3
11/04/98
'4 1 3 11/18/98
'4 2 3 11/18/98
'4 3 3 11/18/98
\45 3
<16 3
12/02/98 12/02/98
v47 3 ' 12/02/98
v49 4
11/03/98
'5 0 4
11/03/98
'5 1 , 4
11/03/98
'5 2 4
11/03/98
<53 4
11/04/98
N54 4
11/04/98
'5 5 4
11/04/98
'5 6 4
11/04/98
'5 7 4
11/18/98
*58 4
11/18/98
<9 4
11/18/98
v61 4
12/02/98
<52 4
12/02/98
'6 3 4 12/02/98
Samples Group Collection Date
-65 5
11/03/98
,,66 5
11/03/98
vfi7 5
11/03/98
'6 8 5
11/03/98
'6 9 5 11/04/98
'7 0 5
11/04/98
'7 1 s 5
<72 5
11/04/98 11/04/98
'7 3 5 11/18/98
'7 4 5
11/18/98
*75 5 '7 7 5
11/18/98 12/02/98
*78 5 . 12/02/98
"79 5 ' 12/02/98
'81 6
11/03/98
^82 . 6
11/03/98
'8 3 6 11/03/98
-84 6
11/03/98
^ 5 6 11/04/98
-86 6
11/04/98
'i l 6 11/04/98
-88 6
11/04/98
*89 6
11/18/98
-90 6
11/18/98
^ 1 6 11/18/98
*-93 6 '9 4 6
12/02/98 12/02/98
'9 5 6 12/02/98
^97 1
11/05/98
'9 8 1 11/05/98
'9 9 1 11/05/98
<00 1
11/05/98
J 01 1
11/06/98
'102 1
11/06/98
'103 1 \104 1
11/06/98 11/06/98
'1 0 5
1
11/20/98
n J06
1
11/20/98
'1 0 7 1
11/20/98
<09 1
12/04/98
'110 1
12/04/98
<11 1
12/04/98
-113
2
11/05/98
<14 2
11/05/98
115 2
11/05/98
<16 2
11/05/98
'1 1 7 2
11/06/98
'1 1 8 2 1 11/06/98
<19 2
11/06/98
'120 2
11/06/98
'121 2
11/20/98
'12 2 2
11/20/98
'1 2 3 2
11/20/98
'125 2
12/04/98
M26 2
12/04/98
'1 2 7 2
12/04/98
Samples Group Collection Date
M 29
3
11/05/98
-130 3
11/05/98
'131 3
11/05/98
-132 3
11/05/98
-133 3
11/06/98
'1 3 4
3
11/06/98
'1 3 5 3
11/06/98
<36 3
11/06/98
<37 3
11/20/98
'1 3 8 3
11/20/98
<39 3
11/20/98
'141 3
12/04/98
< 4 2 3 12/04/98
'143 3
12/04/98
'1 4 5 4
11/05/98
'1 4 6 4
11/05/98
-147 4
11/05/98
*448
4
11/05/98
<49 4
11/06/98
<50 4
11/06/98
-151
4
11/06/98
-152
4
11/06/98
M53
4
11/20/98 .
<54 4
11/20/98
M 55
4
11/20/98
'1 5 7 4
12/04/98
<58 4
12/04/98
-159 4
12/04/98
<61 5
11/05/98
M 62
5
11/05/98
<63 5
11/05/98
-164 5
11/05/98
'165 5
11/06/98
'1 6 6 5
fl/06/98
'1 6 7 5
11/06/98
'1 6 8 5 ' 11/06/98
<69 5
11/20/98
-170 5
11/20/98
<71 5
11/20/98
* '< 7 3
5
12/04/98
<74 5
12/04/98
<75 5 ' 12/04/98
'177 6
11/05/98
<78 6
11/05/98
'1 7 9 6
11/05/98
-180
6
11/05/98
<81 6
11/06/98
<82 6
11/06/98
'183 6
11/06/98
n184
6
11/06/98
J85 6
11/20/98
. <86
6
11/20/98
<87 6
11/20/98
<89 6
12/04/98
<90 6
12/04/98
<91 6
12/04/98
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BACK TO MAIN
iiltU TIO N S SUMMARY SHEET
- n t p J s'W
Sobeptm* Numbers
=>9
Notes:
/.s' *
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BACK TO MAIN
DILUTIONSsummarysheet
SotopCTN Number: Matrix: \a k r \v cr
7* ^ * a - ?
N otes:
BACK TO MAIN
DILUTIONS SUMMARY SHEET
Study: T o * / t o
, , ,,, ,
Dilution Date/Analyst: sf/*/?'}/fi **
Box#: 9%3?}?,
. sample number . o r descrpition
too, lo tf- S f (C lf( .if(01b[ iffIbisF" to ? f-
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B A C K TO M AIN
DILUTIONS SUMMARY SHEET
Study: Dilution Date/Analyst: U Box#: p -O LY A il t
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Matrix/Tlmepoint: fle tte r
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BACK TO MAIN
DILUTIONS SUMMARY SHEET
Study: 70K-/2 O
Dilution Date/Analyst: S 1191,fA r
Box#: 99'O CYjhA
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Solvent/TN Number: M dt, fc 'A -3H T
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26/___ / 33/1 Al 3M 35/
3(.rf
37/
..3r^. 37/ VO/ i 37/ 2
37/
30^
3// ^ 42/ 63
43/ ^5/ 46/
____________________
J
J/
/
x `/% \/
*
Notes: A ( : 2 f ' H O ^ L /: i d -
Sc.~fk
M^OK
.* 9 0 Q / - 1 - / u r i !
. ..
'
BACK TO MAIN
DILUTIONS SUMMARY SHEET
J
Study: -f'K-tQ.O
_
Dilution Date/Analyst: ^ \ ! "t W r
Box#: Jf-OCtiAyQ
Solvent/TN Number: /M U, -taj- A - W t
Extraction Date/Analyst: fiAfir /V?. f/tf Jc/ rpp a. ,,
Matrix/Timepoint: P a t t e r
*
sample number
DILUTIONS
or deserpilion 1UD I: 1: I: 1: 1: 1: 1: . 1: Comments
' LI7M 6 3
\
*
*
*
,
!
/ *
. *.
Notes: # ,( d o s OO^L To-yl^
( ' 7 o/*1
4
*
' .*
*
*
.
#
Study:'tox- o o DOntion Date/Analyst: s-s>n-97 f
Box#; W - o io & A f b
sample number or desrrpition
V//*1 J
1: / 0 b io
1:
BACK TO MAIN
DILUTIONS SUMMARY SHEET
, Sofrent/TNNumber: nuoti(tm-*-pjtoc,
Extraction Date/Analyst: V '/f-
mw- j q - j */*- j ,,
Matrb/nniepoint! doj
DILUTIONS 1: 1: 1: 1: 1: 1: Comments
S-91-<?? S**-'
.
.
,,
/ *
0 *V
Notes: (M O to
1
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y
*
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.
*
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BACK TO MAIN
DILUTIONS SUMMARY SHEET
BACK TO MAIN
DILUTIONS SUMMARY SHEET
Study: TbY'/^O Dilution Date/Analyst:
Bx#; g oy 99-W A * &
//J
J
_________________
sam p le n u m b e r
. o r descrpition
/ o 1:
1: 1:
Mm
SOM
w 0 /6
6H 1
m &H
SM 6V
sm oh 9 iM M
SO I 6 4
(rH V L ___
Solvent/TN Number: M t fl / T*!~A ^ 3 2
/ Extraction Date/Analyst: tJI f f a l J yCAiiJfccP/Sf/SAtJ
_________ M atrix/Tim epoint: f r / fte r / f r U f Y ______________
DILUTION S 1: 1: 1: 1: 1: Comments
*
/
N o tes:
QI0-/OOmC SW pb t 9mL frM to o !
-
,*
'
.
-
*
BACK TO MAIN
Sample weigJbits/Volumes
Prep Date(s):
Analyses): fttfkJ- JCi9"~
Sample Matrix: / ; r
Method/Revision: / ' ''l * -f, O
Target Analyte(s):
/
Study Number
/ 2-0
Equipment Number _
Notebook# and Page:
Final Solvent/TN Number
Sample ID
Initial Wt/VoL g/mL/L
ftnlM Q Water Added
Density(g/ml) Comments
1MG1 2MG1 3MG1 4M Gl SMG1
] 6MG1
. '
6'flTT 3 3 /.9,/* f f ? 3 . 5 " ftr ff -2-- tCe>
A.62X-L 3 M A.92UO H . S ,,
7MG1 8MG1 9MG1
A). 3 9/L5 /i.J T U .7 0.G 3W
H fo
q. ! 3.X
'-
10MG1
# 9 7 2 3 .5
11MG1
V.3
^ ----
13MG1
'
Z . T / 5M
14M Gl 15MG1 17MG2
M /W 4 ftftr s //
A573
V.!T V -3 r.3
>
18MG2 19MG2 20MG2
'
/7.W 93 /?,SX 39
5:0
p .?
3 .7
21M G2
22MG2
J 23MG2 24MG2
/. 3 fo
0 7avr
o.STVo
'
H.O
*3 3C 3.0
'! *.
25MG2
'
26MG2
27M G2 oummary oimcmoa;
OfCrf
3>r
n.-73fC 3 .7
0.9/7 *
V.C
] fill
p r s t i ed
b y P *10
A lt -n * filer- Aexpo?, *
CJ. ^ FAsT-n-J'O
h /Z./
us*
f eluder
( M J * y fJ j r r t*?er j // 9 U ytU ) e -X'*yt t* )
Notes: J & Ad/ rtf
/ /. r
''
\
]. Extraction-1 Template Review Date________
Form Approved by/Date:. \
Weight Volume Sheetl20.xls
Page 1 of
Prep Date(s):
: Analyst(s): AtJhjep'J'AP
Sample Matrix: / f j
^ Method/Revision: FAfT--fl-ftQ
Target Analyte(s):
. Sample ID
29MG2
30MG2
31MG2
33MG3
34MG3
35M G3
36MG3
37MG3
38MG3
39MG3
40MG3
41M G3
42M G3
.
43M G3
45MG3
46MG3
47MG3
49MG4
50MG4
51MG4
52MG4
53MG4
54MG4
'
55MG4
56MG4 Summary of method:
]
.
Sample weightsolumes
Study Number: J~0)Cf p
Equipment Number Notebook# and Page: * Final Solvent/TN Number
BACK TO MAIN
Initial Wt/VoL g/mL/L
#mlMQ Water Added
Density(g/mi)
/.ffSS' s w
lu w r 327 3.H
t.E m > St^HS.O t.tu ia s */./*
3 .0 a .si-w
3 .
/. /tt
0.73C/S
/.>U7-
5 '. V 3.-9 /.S'
S, 3
w
HA
3. i
0.T-/73 3 .L rt-iV/S- 3 , 2
.SS77, 2 . . S
ZA .o
)
)
:
Comments
.Y S-/9 S ..3
I i
Notes:
D
ee
xze
Fonn Approved by/Date:.
Extraction-1 Template' Review Date____________ _
Weight Volume Sheetl20.xls
Page 1 of
BACK TO MAIN
; . Sample weights/Volumes
PrepDate(s):
j A n a l y s t ( s ) : / i ^ ^ - J r / 7-' jfi- P ' Sample Matrix: /?t l-l-t*'/
Method/Revision: f^A-rTz-/7~/fO
j Target Analyte(s):
. Sample ID
57M G4
58M G4 .
59M G4
61MG4
62MG4
63MG4
65MG5
66MG5
67MG5
68MG5
69MG3
70MG5
71MG5
72MG5
73MG5
74MG5
75MG5
77MG5
78MG5
79MG5
81MG6
82MG6
83MG6
84MG6
85MG6 Summary of method:
]
Study Number: *7"
&
' Equipment Number:
Notebook# and Page: *
.
FinalSolvent/TNNumber: -
Initial Wt/VoL g/mL/L
0 .7 3?
s.qnt 0.19S
/. rrub> .onTX 0.90.3 la m o.70( D/9T73
O&Y 0.9377 o-tfiod 0.93/C t.ourc /. 007 / P'i%C 0.9900 0.993 0.73d? f.o9o3
om cb 0.9703 o.llQ f t.o n z
#mlMQ Water Added
H .r H.c
? .4 "
ITV
3M 9.3 32. 9.0 (/.9 31 9.9
m. y 3.3 31 3.0 30 30
3 .*
3.3 97 .H.7 v.q
3 .9 *3
Density(g/ml) Comments
J Notes:
!
J Form Approved by/Date:.
Extraction*1 Template Review Date___________ _
Weight Volume Sheetl20jds
Page 1 of.
BACK TO MAIN
Sample weights/Volumes
Prep Date(s):
: Analyses):
-)
1 Sample Matrix: fhf-
Method/Revision: p fa 7-V /7 - / , p
] Target Anaiyte(s):
. Sample
ID
86MG6
87MG6
] 88MG6
89MG6
90MG6
] 91MG6
93MG6
1 94MG6 95MG6
'
1
Study Number: "y~OTf~fXO
Equipment Number: Notebook# and Page: . * . Final Solvent/TN Number
Initial
#mlMQ Water
Density(g/ml)
Wt/VoL
Added
(ghnL/L
v:* &.Z37Z V r Z
*,97Lt _ 4,4
l'ZCv S .Y
t - o t t i s~,3
t'0T33
9oJZ Y .s:
aAo
IrOt-H jr ,l
/,/ 0 J 7 S<S~
..
Comments
J
I
i
J J
Summary of method:
]
j Notes:
%
.
j"
I.
J Extraction-1 Template Review Date___________
!
Form Approved by/Date:.
. Weight Volume Sheetl20.xls
Page 1 of,
BACK TO MAIN
Extraction Worksheet ETS-8-4.0
Study * - r a * f i,c
Surrogate Std
FC-Mix
Box U
A-i g approx ppm approx. 0.5 ppm
Matrix
a c t u a l p p m actual ppm
1___ n ? 9 > //^ 2
9__ -a XlW l- ___ ___________ _ ' --/U4L
-
SflA --/v 2--
-2*1__________
-i-k i. -.'r lA L
f f l. ____ / a J
~Jaoapl-t ir
FC-Mix approx. 5 ppm actual ppm # ? ? *//-/-!
-
FC-Mix approx. 50 ppm actual ppm
-
-.
Date and Initials for
Std. or Comments
------ 1
'!
1_ _A--------- 1---- 77-------
M Cl____________
H M 6d___________ : m w m m am m SM C-1 LM ir !
V/
m &
9m 6-1
M 6-1
liM6-1 m 1 6! MMM
ISM # /
MM M mu
-Ac------------
- '
'IAJ. ID J fij-
-
-
-
-
jj .
VF --
DcP-sap-U JI i"
*
'
.
- . -
.-
.
-
- -
- . . .
- :.
I'
-
.
_-
.# 1
-
#- .
.-
...
-Study num ber where the original worksheet is located.
_Blank AU
Serum Extraction Method
Std * M o n - f - l r *
amount
itO
P_________ * t t f r - M - t , _______ :
___mL A j / 7 i E . g / ) ' . Dat & Initials-
Vortex 15 sec.
V /rt/9 9
______.
Pioette M atrix. Pipette 1 ml of 0.5 M TBA, pH 10.
Volume
/,
ml
Std.# 99o,f-^2_ .
-------------- ----------- v K l -- -
. sm izu jU m m .
Pipette 2 ml of 0.25 Na7COV0.25M NaHCOi buffer Std. # 99 0 / / - /S~A '
Dispense 5 ml o f methyl-t-butyl ether
TN-A- XT*
"
Shake 20 min. Centrifuge 20-25 min.
Centrifuge speed: 7T&&
(Rem ove a 4 mL aliquot of organic laver
Put on Nitrogen Evaporator to drvness Evaporator # :
Temperature:
Add methanol
Volume 1.O ------- ml--------------- TZT3& & -------
/
1 Vortex 30 sec.
1
IFilter using a 3cc B-D syringe with a 0.2um filter into a 1.5 mi autosamole vial
MS/MSD/ Cont. Checks: Spiked fo uL of a ppm std ( *7?>//-/.-! ) for a final concentration of
/f .lf f i. ppm. MS/MSD used sample flj)
. Cont. Checks used same matrix as for std curve.
Surrogate Standard: Spiked jo uL of a S~ ppm std f 99o/}-/>-! )to all samples, standards, and blanks
Q) f t J / h l U J r t 6 l }
A ttach m en t'A
ETS-8-4.0 Extraction o f PFOS from Serum
Page 10 o f 13
BACK TO MAIN
Extraction Worksheet ETS-8-4.0
Study #TG)f-130
SurrogateStd
FC-Mix
Box #9
approx. ppm approx.0.5ppm
Matrix Liter actualJOO ppm actual ppm
Woia-3^ #
1 1 M t o -_______ S"j i L
-
to -
____ 1_______
.-
m to -
FC-Mix
approx.5 ppm actual ppm #
-
FC-Mix approx.50 ppm actual ppm
#
-
-
Date and Initialsfor
Std.or Comments
1 -----1
a m to-
&2M to -
M M to-
atM to -
M M to -
a m to-
M M to HflAf t o
to
- .
-
aiM to VIM to
-
'-
$A\ to % M to
-
r
(J 3*M to M M 64
-
-
ViM 64 m 1 to 4tf/Vl t o
H i M 64 VSVfl to % M to ?M U
MM p/ U A to/
> L______:
-
-
' -
>.
_!^tudvnumberwheretheoriginalworksheel:islocated._____ ____________
amount= A C1
mL^inOife, M l
Serum ExtractionMethod
_____ '/r r L
Date& Initials 1
Vortex 15 sec. Pipette Matrix
Volume
ml
\ls
Pipette 1 ml o f 0.5 M TBA, pH 10.
Std.#
s w i w u y/jjS
Pipette 2 ml of 0.25 Na?CQV0.2SM NaHCOt buffer Std. # Y9/7/Z-rTF
Dispense 5 ml o f mcthyl-t-butyl ether
TN-A- 2X5T9
Shake 20 min. Centrifiiee 20-25 min.
Centrifuge speed: 3St)f) Sp/W
Remove a 4 mL aliquot of organic layer
Put on Nitrogen Evaporator to drvness Evaporator ft:
Add methanol
Volume LO
ml
______ Temperature: TO-A- g e a r fe>
Vortex 30 sec.
Filter using a 3cc B-D syringe with a 0.2um filter into a 1.5 ml autosamole vial
MS/MSD//f Cont.Checks:Spiked I jiLofa
ppm stdiW a IH L- I Uor afinalconcentrationof
' ppm. MS/MSD usedsample^!'/k e J'j r u J` Cont.Checksusedsame matrixasf'orstd`curve.
SurrogateStandard:Spiked iO uL ofa <T ppm stdf 9^6)IHb-l )toallsamples,standards,andblanks
4)MSi/MPi-spt'u
3 M 6 - 1 1m ) M m - i p u M M , / u t t m a f ' Z
Attachm ent A
ETS-8-4.0 Extraction o f PFOS from Serum
Page 10 o f 13
BACK TO MAIN
Extraction Worksheet ETS-8-4.0
study m r - 1 3 0
SurrogateStd
FC-Mix
FC-Mix
FC-Mix I Dateand
Box approx, pptn approx.0.5ppm approx.5 ppm .approx.50 ppm Initialsfor
Matrixl&f-Uver
actual/00 ppm actual ppm actual ppm actual ppm
Std.or
#990/
#
#
# Comments
1
S b M (t H SMI V-
__ S a i L ____
-
-
-.
rsP-sty-uicAs
f-
I
SVM Mi; C T M V-
wrPr-
1___ ---
&/H v1 StM
6-*f-
J MM M M M (r*f
feOVf C
lM & C 1 M M Mr
MM ^ M M T 1 WlvM 6S~ jm Mr
J JMI (r<T VfM AT JtM M r 17M! tf-C TfM MT MAIMT Y M 6-to t i M irh
> L______
-
-
-. - . -
** '-
-. -
-
-
-
-
'-
-' -
-
__
it
1Studynumberwherethe original worksheet is located.
J min*
unr
I Serum Extraction Method
std # V i o l e t t --------______:
amount = A U -- O______
II 1 III II 1 HI Date & Initials
Vortex 15 sec. Pipette Matrix
- Volume
>\ 0
ml
L'
1 I 1
5
3
Pipette 1 ml of 0.5 M TBA, pH 10.
_____ Std. # GQa/f
Pipette 2 ml of 0.25 Na?COV0.25M NaHCOi buffer S t d . # ^ / tS'O
1Dispense 5 ml of methyl-t-butyl ether
______________ TO-A- Z3-9
] Shake 20 min.
.
1 /
1Centrifuge 20-25 min.
Centrifuge speed:
Sp/Y\
-J
] Remove a 4mL aliauot of organic laver
LPut on Nitrogen Evaporator to drvness Evaporator #:
Temperature:
/
Add methanol
Volume l- 0
ml
TN-A- a W
~
I Vortex 30 sec. Filter using a 3cc B-D syringe with a 0.2um filter into a 1.5 ml autosamole vial
1 "37
J 0 . 3 H ) _ ppm. MS/MSD used sample ) InfJ/ru) . Cont. Checks used same matrix as for std curve.
Surrogate Standard: Spiked 0 uL o f a
ppm std i ff i b ih / l o - / )to all samples, standards, and blanks
<D M S i / m p j - S f i l O i Z M 6rlj A fisa/M S& t-5f)ceU M lf M 3 M S P 3 ' S f i k z B / f t ( r i
i Attachment A
ETS-8-4.0
P age 10 o f 13
Extraction o f PFOS from Serum
Extraction Worksheet ETS-8-4.0
BACK TO MAIN
Study #_7DV-7?
Box n w - ,fA iti Matrix E A f LlVPf
Surrogate Std
approx, ppm actual/00 ppm
#9
0 V fT W . 'B m 1 _____s : MM
ts-l* frU-
. FC-Mix approx. 0.5 ppm actual ppm
# -
FC-Mix
FC-Mix
approx. 5 ppm approx. 50 ppm
actual ppm
actual ppm
#
- '-
--
Date and Initials for
Std. or Comments
JW - s M J *
\
IM I i l -
M l e*U M &r-
m . . M -__________
UM VM m , -
3 9*/M i. KM rt*
3/4t-M _
,C /_ r-Tjr/>
1 --r i r M 6-!
L________
-
-
fo J-
- -
-
'
_
'
-
.
r--
/JM A-t - r r r { 4 MA-t - H rr>
K _
-
__ J T
--
- 9- "
( im s j m p tf & ^ &<* OVUsi 1.
A'TTtruJfLf I'AU Yv -
oU-ipJiJ t\i Mas /o C O t s i h w //!$& ai r t i / 7. SA ti_v/l V f ? -
u )h
w
7(0/1M 'P tQ fh
27
_-
_________ - -- -- * . v-v........
___
_______ -
'"All
^ IL --^
*
1Study number where the original worksheet is located.
J Blank JSflfcfcrf LYtf Serum Extraction Method
Std#
amount' / i) I A cT-M~/. D
mL
Date &Initiab
Vortex 15 sec.
Pipette Matrix Pipette 1 ml of 0.5 M TBA, pH 10.
Volume Std.#
T ml </~2-
~]ZL
Pipette 2 ml o f 0.25Na?CO^/Q.25M NaHCOt buffer Std. # ? g a / / --
] CD Dispense 5 ml ofmcthyl-t-butyl edier Shake 20 min. Centrifuge 20-25 min.
TN-A- I S T Centrifuge speed: 35VO/prt
Remove a 4 mL aliquot of organic laver
Put on Nitrogen Evaporator to drvness Evaporator#:
Add methanol
Volume A 0
ml
Temperature: TN-A- S E S i
Vortex 30 sec.
Filter using a 3cc B-D syringe with a0.2um filter into a 1.5 ml autosample vial
NL/ ~
0`MS/MSD/ 8 C ont Checks: Spiked )0 uL o f a S~ ppm std ( ^ 6 ) / - } U*7 ) for a final concentration o f (7 ) ppm. MS/MSD used sampled?) t,fjA IaJ . Cont. Checks used same matrix as for std curve.
Surrogate Standard: Spiked 10 uL of a A ppm std (
to all samples, standards, and blanks
& M S J . m o h s p > & b M MS3//WP3 - y h flM l
Attachment A
--,JETS-8-4.0 Extraction o f PFOS from Serum
Page 10 o f 13
.
Ion Pair Standard Curves-TOX-120 (Rat Liver)
BACK TO MAIN
~~
Prep Date(s): Analyses): Sample Matrix: Method/Revision:
Target Analyte(s):
4/19/99
SAH/RWW/JCP/SRP
Rat Liver
FACT-M-1.0
.
POAA, PFOS. , PFHS, M513 and M613
FC Mix Std Approx. 0.500 ppm: FC Mix Std Approx. 5.00 ppm: FC Mix Std Approx. 50.0 ppm: Surrogate Std Approx. 100 ppm:
99011-16-2 99011-16-1 99011-124 99012-3-24
! Actual Concentrations ofStandards in the FC Mix
POAA
PFOS
Std Cone. ug/mL
Std Cone. ug/mL
Std Cone. ug/mL
j 0.500 j 0.500
0.500
0.500 0.500 0.500
0.500 0.500 0.500
0.500
I 0.500 j 5.00
5.00
5.00 r . 50.0
0.500 0.500 5.00 5.00 5.00 50.0
0.500 0.500
5.00 5.00 5.00 50.0
PFHS Std Cone.
ug/mL
0.500 0.500 0.500 0.500 0.500 5.00 5.00 5.00 50.0
1
Calculated Concentrations ofStandards in the Sample Matrix.
POAA Final Cone.
PFOS Final Cone.
Final Cone.
PFHS Final Cone.
ng/g ng/g ng/g ng/g
5.56 5.56 5.56 5.56
1 11.1
11.1 11.1 11.1
27.8 27.8 27.8 27.8 i 55.6 . 55.6 55.6 55.6
I ll 111 111 111
! 278
278 i 278
278
556 i 833
556 556 556 833 833 833
1111
J
1111 1111 1111
Study Number: TOX-120 Equipment Numben Final Solvent & TN Number: MeOH/ TN-A-2438 Blank TIssue/IdentifieR Rabbit Liver / 99012-5-20
M513 Std Cone.
ug/mL
0.500 0.500 0.500 0.500 0.500 5.00 5.00 5.00 50.0
M613 Std Cone.
ug/mL
0.500 0.500 0.500 0.500 0.500 5.00 5.00 5.00 50.0
'
ah
Ain't Spiked mL
0.002 0.004 0.010 0.020 0.040 0.010 0.020 0.03 0.004
AU Density
g 0.180 0.180 0.180 0.180 0.180 0.180 0.180 0.180 0.180
M513 Final Cone.
ng/g 5.56 11.1 27.8 55.6
111 278 556 833 1111
M613 Final Cone.
ng/g
5.56 11.1 27.8 55.6
111 278 556 833 1111
Surrogate Std Cone.
ug/mL
100 Surrogate Final Cone.
ng/g 2778
AU Amt Spiked
mL
0.005 Surrogate . Final Cone.
ng/mL
500
1
]
I i
POAAJFOS, .PFHS.M513.M613.xl5
BACK TO MAIN
Ion Pair Extraction Worksheet
Study #
Surrogate Std
FC M ix Std
FC M ix Std
FC M ix Std
D ate and
t *4
' approx.
actual
m
ppm ppm
approx. 0.5 ppm
actual
ppm
# w 9?o/t-/o-x
approx. 5 ppm
actual ppm
#W tfo/Ui-l
approx. 50 ppm
actual
ppm
# w 99o?/*x*t
Initials for Std. or
Com m ents
H ,0 B lank -[4 1ilAi,4- L>*er B lan k -/?,
S~,J
" zu tt ti-
Qrf/ A l
1' /
--f,,A- _ --
-- 1-------------- t t r f--
---------------3 s ! 5 ? r
__________
V J , ...
[o j, 2oJ.
...
-*
-Li-In?'.A-
V*J
oJ
.
Soo'.A -
' StA-
] ___ J /
-L l-, ~2sb.A-Ofti, nr
___
ptJ
3e>J
j-----------
(OJ
Y
'
4/,)"aA* lo/.fa.---/i-l-2TWt. .M--S X ~}n.A Hfi)
J*JL________
-
f)MO*.1IDP~-(5`)~'2SJ, MS X -^ > ,.1 tlSD ---------1-----------
t o d ,_______ iZ j
--
t~ tfeiut-f f)\ oeS" /-*r
/ / / ra n >
1-------
. .1
/r M r
. ^ \cf) f F r 4----------- 1
_ -- -- __ .-- -- "
_ _ -- -- ^
^ ,-- "
----
------------
-- ---------------------
J ` -- :-- :-- Blank BAiitc,uer
_-- - -- ------------------------
Std# ?X0?9-/S- 3
amount - /.Oa.t
rt./c?*
i Extraction Method Number/Rcvision:
____________ ___
< Date &In itials^
Add Surrogate. Vortex 15 sec.
--------- --------------------- .----- ------------fnr4
WrJFSHSW
Pipette Sample
Volume
ft V m L --------------- ,----------- ------------ .
........ - r ...,
Pipette l mL of 0.5 M TBA. pH 10.
Std. # 7*09< f A
------------- -------------------------
] Pipette 2mLof 0.25 NapCCh/0.25M NaHCOi buffer Std. # <79OH-*? >>_
Pipette 5 mL ofrcthyf3eetatc
AmJ-A/xtJfi TN-A- -2A===-
......................................
-------------
--
Shake 20 min. Centrifuge 20-25 min.
_____
* -------------
________ r.entrifiipc speed:
-------------
------------
-------
... --
Remove a 4 mL aliquot of organic layer Put on Nitrogen Evaporator to drvness Evaporator#:
---------------------------- ----------- ------------------
Temperature:
A* **/.
. -
Vortex 30 sec.
________
-- --
Filter using a 3cc B-D svringe with a 0.2um SRI filter into a 1.5 mL autosample vial . . . _ .
Aro
Additional Comments:
-- ~r~
A----------- ^ ---------------
oy
[
_____________________________________ J
. Ion pir Standard Curves-TOX-120 (RatLiver)
BACK TO MAIN
Prep Date(s):
2/22/99
Analyses):
RWW/SAH
Sample Matrix: Rat Liver
Method/Revision: FACT-M-1.0
Target Analyte(s): POAA, PFOS,
PFHS.M513&M613
Study Number: TOX-120 Equipment Number: Final Solvent A TN Number MeOH/TN-A-2325 Blank Tlssue/Identiflen Rabbit L iver/98099-15-3
FC Mix Std Approx. 0.500 ppm: FC Mix Std Approx. 5.00 ppm: FC Mix Std Approx. 50.0 ppm: Surrogate Std Approx. 16.5 ppm:
99011-16-2 99011-16-1 99011-124 98094-12-24
.
Actual Concentrations o f Standards in the FC Mix
POAA
PFOS
PFHS
M513
M613
AU
All
Std Cone.
Std Cone.
Std Cone.
Std Cone.
Std Cone.
Std Cone.
Ain't Spiked
Density
1
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
ug/mL
mL
8
0.500
0.500
0.500 .
0.500 '
0.500
0.500
0.002
0.167
0.500
0.500
0.500
0.500
0.500
0.500
0.004
0.167
] 0.500 0.500
0.500 0.500
0.500 0.500
0.500 0.500
0.500 0.500
0.500 0.500
0.010 0.020
0.167 0.167
.
0.500
0.500
0.500
0.500
0.500
0.500
0.040
0.167
5.00
5.00
5.00 5.00 5.00 5.00
0.010
0.167
5.00 5.00 5.00 5.00 5.00 5.00 0.020 0.167
1
5.00
5.00
5.00 5.00 5.00 5.00
0.030
0.167
SO.O
50.0
50.0 50.0 50.0 50.0
0.004
0.167
1 '
Calculated Concentrations of Standards in the Sample Matrix
POAA
PFOS
PFHS
M5X3
M613
Surrogate
Final Cone.
Final.Cone
Final Cone.
Final Cone.
Final Cone.
Final Cone.
Std Cone
ng/g ng/g ng/g ng/g ng/g
m ug/mL
5.99
5.99 5.99 5.99 5.99 5.99
16.5
12.0 12.0 12.0 12.0 12.0 12.0 Surrogate
29.9 29.9 29.9 29.9 29.9 29.9 Final Cone.
59.9 59.9 59.9 59.9 59.9 59.9 ng/g
120 120 120 120 120 120 494
299 299 299 299 299 299
599 599 599 599 599 599
898 898 898 898 898 898
1198 1198 1198 1198 1198 1198
J
!
M S/M SD
106F-G1 110F-G1
POAA
Final Cone
____ ng/g
J 106F-G1 110F-G1 !
299 299
Initial W t feV
0.9240 0.9242
PFOS Final Cone .
. ng/g 299 299
Initial V o lu m e tmt.V
4.6 . 4.6
Final Cone ng/g 299 299
PFHS Final Cone
ng/g
299 299
MS13 Final Cone
299 299
M613x Final Cone
ng/g 299 299
Amt Spiked fmLV 0.010
0.010
A ll Amt Spiked
mL
0.005
POAA.PFOS, .PFHS.M513.M613
Sample Weights/ Volumes
PrepDate(s): 2 ///if 1Analyses): fau
SampleMatrix: fl.}Lu?/ Method/Revislon: /Vi<7'-/V7- I'V
TargetAnalytefs):
StudyNumber: EquipmentNumber: Notebook# and Page: FinalSolvent: /7
/XO
.
r<X
Sample ID
Initial
(@rvoL /SmIVL.
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Comments
gmlrtiL
O./bl .IL& n./is
O 'I 0 0 -----n.iOO .t(o4 f).(00 ./0S1______ 0: 6.11*3 '
0./.T
n.ibb QJJt - ______ 6.11*3
"O.OO (\U*7 6.1 or ojbr 6.100 C U 70 6,100 .M
.
Summary ofMethod:
]
BACK TO MAIN
j Notes:
J
Extraction-1 Template Review Date________
Form Approved by/Date:. Weight Volume Sheetxls
Page 1 of
Sample W eights/ Volumes
BACK TO MAIN '
Prep Date(s): *z/ 1% Analyst^): fcUJU] /SAM 'Sample Matrix:jtf- LiFtf Method/Revision: p/fjZ./V[ ~J,Q
Target nalyte(s):
Study Number: '()(--/ P
Equipment Number:
Notebooks and Page:
Final S o l r e n t : ^ / / / ; '. uJaUtr
Sample ID
Initial $pVoL
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/0/lk I M F - & 3 ClAIa IZrF-irX " D aJ/). Id (jp~ DAln. 13 F F - P I OaIa .13 9 P - 6-3 ' A)a .136 F-P i PNn. M F - 6-3
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3 /m l
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t u e , '____ o. Jio9 (MUT-
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J5H n.n.' 9
O.Jfdfr O.Mob
OMt 6.M fJUt
..
O.M M O ? ____ ,
J, -
J Summary of Method:
I Notes:
J
. Extraction-1 Template Review Date____________
I
^ F o r m Approved by/Date:_______ ___________________ --
Weight Volume Sheetxls
Pa8e * *
Sample W eights/ Volumes
BACK TO MAIN
Prep Date(s): /if/?*)
wdyrtsy.
r
pample Matrici /lif-Lt
Method/Revidon:
Target nalyte(s):
6
Study Number: / OA-/
Equipment Numben
Notebook# and Page:
Final Solvent: fi,//,
?/ 'S
Sample ID
Initial @fVoL /#mI7L
tw i M akr
d tf t '/j O r f/r tL
Comments
D r/n ir ft
-----_'_--_-_--_-_--_-_--_-_--
f i J f f , f 7 7 - p - -- S r n __________ 6 ' C 3 1 \
- ...Jn/ f^T/U) t.u tirr itr ik ? kLmmf........ - 1{n / (mT i L?J, f/ / y hr.-f
f ) / r $ T ~ ~ --- * - / *______
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f t u a , t C / X - / S-5"
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f t t a , //. X - A - X
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f ld b
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I Summary of Method:
'f .
Notes:
Extraction-1 Template Review Date.____________
Form Approved by/Date:.--------------------------------------- -
Weight Volume Sheetxis
Page lo i
Sample Weights/Volumes
Prep Date(s): i f i f fa ?
Analyses):
Atf
Sample Matrix: A *//< <' sr
Method/RevUlon: /Q f/ 7 '-/H7 - / ,
Target Xnalyte(s):
Study Numben *J~0/ - / LQ
Equipment Number:
Notebook# and Page:
.
Final Solvent: fit ll, # V*. 7*f
Sample
ID
Initial
\hf*4*r
k/VoL
OfxnUL .... ft^ l
$ m/ m C
pA/a. 7 9 ?
P'SVSJ- . 7 ~ n 7 lW
PNO. ( f t f - 4 Drifi. [ ZLf - 6 6
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trCTf? J ^ f
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6,9r id
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OJU,
Comments
BACK TO MAIN
J
Summary of Method:
]
rr
I!
Extraction-1 Template Review Date____________
Form Approved by/Date:___________________________
' i_
Weight Volume Sheetxls
Page 1 of
BACK TO MAIN
SOUP 020199: FACT Equipment Log 99014
Quattro II, #6414E
PAGE
DATE: 2/23/99
ANALYST: drb
STUDY NUMBER:Tox-120 MATRIX: Rat Liver DESCRIPTION: Liver Sam ples.
FACT Method: FACT-M-4.0 .
Injection volume: 10 uL
Cycle time: 13.5 min.
Electrode: Z-Sprav
MobDe Phase:
fA12.0 mM NH4CH3CQ2
OBIM cO H
TN-A- or stndrd number (A) 98012-1-4_________
fBl TN-A-2260
Prepared (date/by): Expires:
(A1 2/11/99 hoi________
(A) 8/99 ____________
(B1 2799. hoi
(B) 8/99
Store at room temp? @ or N
Other:___
G u ard colunm: None__________________
Part number: na_________
Lot number: na
A nalytical column: Keystone Betasil____________________________________
Size: 2 x 100 mm________ - : ' Type: C l 8____________ Sphere size: 5 um_______
Part num ber 105-701-2__________ Lot number: P8C09
Serial number: 10881106D
Analytical param eters:
Drying gas (L/hrl: 300
.
ESI nebulizing gas (L/hrl 15
LC Flow rate: 300 uT/tnin. Back pressure: 120 bar
Sheath gas (L/hrl 0
.S
'
HPLC solvent gradient: Long 2
Time (min.l 110-00_____________
2 ) j i5 Q __________
3111.0 _______
4112.0 51 13.5_____________
%B 40.0
mo.
90.0
40.0 Stop
-
Tuning Method Report
Method:
C :\MASSLYNX\FACT-1.PRO\ACQUDB\PFOS
Printed:
Tue Feb 23 15:59:26 1999 _
____________________________________________;___________ Jouj) c a o m
Ot
IAC o i l olii
BACK TO MAIN Page 1
, SOURCE ( ESP- ) 1] Capillary
Cone Extractor I RF Lens i Source Block Temp.
Desolvation Temp.
1
| Pressures
} Analyser Vacuum Gas Cell
J1 .
!
J
.i!
J
Set Rdbk
3.00
60
5
0.2 150 250
2.99 -60 -6
142 250
Rdbk
7 . 8e-6 FAIL
MSI
LM Resolution HM Resolution Ion Energy Ion Energy Ramp Lens 6 Multiplier
Set Rdbk
. 14.0 14.0 *' 2.0 0.0 55 650
MS2
LM Resolution HM Resolution Collision Ion Energy Ion Energy Ramp Lens 8 Lens 9 Multiplier
Set Rdbk
15.0 15.0 45 2.0 0.0 40
0 650
42
39 44
I
]
1
]
1 1
Printed!
Tu Feb 33 1 8 : O O U 3 199
Suj) 20M __
Solvent Dly ( mins ) i
0 .0 0
Function t 1
KRH of 3 HaS a f a i n ( U P - J
Inter Channel Delay ( Secs ) i
Span ( Daltons ) i Start T i m ( Mins ) t End T i m ( Mins ) t Repeats t
Channel Parent Daughter
0.03
0.00 0.00 13.50 1 Dwell (Secs) Coll Energy (eV)
Cone ( V )
1
399.00 80.00
0.25
2
399.00 99.00
0.25
3
399.00 130.00
0.25
45 45 45
50 50
60
(AC os/loll
Function t 2
M W of 3 Mass Fairs ( U P - >
Inter Channel Delay ( Secs ) t Span ( Daltons ) t Start T i m ( Kins ) t End T i m ( Mins ) i Repeats *
Channel. Parent Daughter
0.03 0.00 0.00 13.50
1 Dwell
.
(Secs) Coll Energy (eV)
Cone
1
499.00 80.00
0.25
2
499.00 99.00
0.25
3
499.00 130.00
0.25
45 45 45
60
60 60
Function i 3
XXM of 2 Maas Fairs ( ESP- )
Inter Channel Delay ( Secs ) i
Span ( Daltons } i Start T i m ( Mins ) s End T i m ( Mins ) ; Repeats $
Channel Parent Daughter
0.03 0.00 0.00 13.50
1.
Dwell (Secs) Coll Energy (eV)
Cone
1 299.00 80.00 ' 0.25 2 299.00 99.00 0.25
45 45
35 35
V)
Function i 4
MRX of 3 Mass Fairs ( ESP- }
1
Inter Channel Delay ( Secs ) i 0.03
I
Span ( Daltons ) t Start T i m ( Kins ) < *
0.00 0.00
End T i m ( Mins ) I Repeats t
13.50
1
Channel Parent Daughter Dwell (Secs) Coll Energy (eV) Cone ( V )
]1 2
413.00 119.00 413.00 169.00
0.25 0.25
3
413.00 2 19r00
0.25
30 20 30
15 15 15
Function i 5
KRK Of 3 Maas Palra ( n r - )
J Inter Channel Delay ( Seca ) t 0.03
Span ( Daltons }
t 0.00
Start T i m ( Mins ) t
0.00
End T i m ( Kins ) t
13.50
Repeats i
1
Channel Parent Daughter Dwell (Sk i I Coll Energy (eV) Cone
11
469.00 169.00
0.25
45
. 30
'2
469.00 219.00
0.25
45
' 30
3
469.00 119.00
0.25
45
30
]
1
1
:
i
rmetloB 1
m of 3 M u .
>
Int.r Channel Dl.y ( Secs ) t Span { Dalton. ) i
Stare Tin. ( Kin. ) i
Bnd Tia m ( Kin. ) i
Repeat. i
>
Channel Parent Daughter
0.03 0.00 0.00 13.50 1 Dwell (Seca) Coll Energy (eV)
Cone
1
569.00 169.00
0.25
3
569.00 219.00
0.25
3
. 569.00 119.00
0.25
45 45
45
35 35 35
Function i 7
m m of 1 M u a Fair ( w - )
Intar Channel Dalay ( Saoa ) i S p u ( Dalton. ) > Stare Tina ( Min. ) i *nd Tim. ( Mina )' i Sapaata t
Channel Parant Daughter
0.03 0.00 0 .Q0 13.50
1 Dwell
(Seca) Coll Energy
(eV)
Cota ( V
1
627.00 90.00
0.25
40
60
BACK TO MAIN
BACK TO MAIN
M asslynx - Sam ple List
Sample List: C:\MASSLYNX\FACT-1.PRO\SampleDB\022399c.SPL
Printed: Tue Feb 23 16:25:201999
~
in a
_____________________________________ S n u p 0 2 0 L M
,i
LAC, o s / b i n
Page 1
File Nam e File Text
.
MS R ie Inlet 1 Botti olum e Sam ple T] C o n c A C o n c B C o n c C
1 022399020 MeOH Blank
TOX12C
2 022399021 MeOH Blank
TOX12C
3 022399022 Tox 120, RBL02229-H2O Blk-1,2 /2 2 TOX12C
4 022399023 Tox 120, RBL02229-RBLiverBlk-1,2 , TOX12C
5 022399024 Tox 120, RBL02229-5ppb-1, 2/22/99 TOX12C
6 022399025 Tox 120, RBL02229-10ppb-1,2/22/9 TOX12C 7 022399026 Tox 120, RBL02229-25ppb-1,2/22/9 TO X2C
8 022399027 Tox 120, RBL02229-50ppb-1,2 /2 2 /9 TOX12C
9 022399028 Tox 120, RBLQ2229-100ppb-1, 2/22/ TOX12C
10 022399029 Tox 120, RBL02229-250ppb-1,2/22/ TOX12C
11 022399030 Tox 120, RBL02229-500ppb-1,2/22/ TOX12C
12 022399031 Tox 120, RBL02229-750ppb-1,2 /2 2 / TOX12C
13 022399032 Tox 120, RBL02229-1000ppb-1,2/22 TOX12C
14 022399033 MeOH Blank
TOX12C
15 022399034 Tox 120 DNO. 97F-G1, 2/22/99
TOX12C
16 022399035 Tox 120 DNO. 98F-G1, 2/22/99
TOX12C
17 022399036 Tox 120 DNO. 99F-G1, 2/22/99
TOX12C
18 022399037 Tox 120 DNO. 100F-G 1,2/22/99
TOX12C
19 022399Q38 Tox 120 DNO. 101F-G1,2 /2 2/9 9
TOX12C
20 022399039 Tox 120, RBL02229-250ppb-CC1,2 / TOX12C
21 022399040 MeOH Blank
TOX12C
22 022399041 Tox 120 DNO. 102F-G1, 2/22/99
TOX12C
23 022399042 Tox 120 DNO. 103F-G 1,2/22/99
TOX12C
24 022399043 Tox 120 DNO. 104F-G 1,2/22/99
TOX12C
25 022399044 Tox 120 DNO. 105F-G1, 2/22/99
TOX12C
26 022399045 Tox 120 DNO. 106F-G 1,2/22/99
TOX12C
27 022399046 Tox 120, RBL02229-250ppb-CC2, 21 TOX12C
28 022399047 MeOH Blank
TOX12C
29 022399048 Tox 120 DNO. 107F-G1, 2/22/99
TOX12C
30 022399049 Tox 120 DNO. 109F-G1, 2/22/99
TOX12C
31 022399050 Tox 120 DNO. 110F-G 1,2/22/99
TOX12C
32 022399051 Tox 120 DNO. 111F-G1,2 /2 2/9 9
TOX12C
33 022399052 Tox 120, RBL02229-250ppb-CC3, 21 TOX12C
34 022399053 MeOH Blank
TOX12C
35 022399054 Tox 120 DNO. 113 F -G 2,2/22/99
36 022399055 Tox 120 DNO. 114F-G2,2/22/99
TOX12C
TOX12C
37 022399056 Tox 120 DNO. 115F-G2, 2/22/99
TOX12C
38 022399057 Tox 120 DNO. 116F-G2, 2/22/99
TOX12C
39 022399058 Tox 120 DNO. 117F-G 2,2/22/99
TOX12C
40 022399059 Tox 120, RBL02229-250ppb-CC4, 21 TOX12C
41 022399060 MeOH Blank
TOX12C
42 022399061 Tox 120 DNO. 118F-G 2,2/22/99
TOX12C
43 022399062 Tox 120 DNO. 119F-G 2,2/22/99
TOX12C
4 4 . 022399063 Tox 120 DNO. 120F-G 2,2/22/99
TOX12C
45 022399064 Tox 120 DNO. 121F-G 2,2/22/99
TOX12C
46 022399065 Tox 120 DNO. 122F-G 2,2/22/99
TOX12C
47 022399066 Tox 120, RBL02229-250ppb-CC5, 21 TOX12C
4 8 022399067 MeOH Blank
TOX12C
49 022399068 Tox 120 DNO. 123F-G 2,2/22/99
TOX12C
50 022399069 Tox 120 DNO. 125F-G 2,2/22/99
TOX12C
51 022399070 Tox 120 DNO. 126F-G 2,2/22/99
TOX12C
52 022399071 Tox 120 DNO. 127F-G 2,2/22/99
TOX12C
53 022399072 Tox 120 DNO. 129F-G 3,2/22/99
TOX12C
54 022399073 Tox 120, RBL02229-250ppb-CC6, 21 TOX12C
55 022399074 MeOH Blank
TOX12C
56 022399075 Tox 120 DNO. 130F-G 3,2/22/99
TOX12C
57 022399076 Tox 120 DNO. 131F-G 3,2/22/99
TOX12C
58 022399077 Tox 120 DNO. 132F-G 3,2/22/99
TOX12C
59 022399078 Tox 120 DNO. 133F-G 3,2/22/99
TOX12C
long2 long2 long2 long2 !ong2 long2 long2 long2 long2 long2 long2 long2 long2 long2 long2 long2 long2 long2 long2 long2 long2 long2 long2 long2 long2 long2 long2 long2 long2 long2 long2 long2 long2 long2 long2 long2 long2 long2
long2 long2 long2 long2 long2 long2 long2 long2 long2 long2 1ong2 long2 long2 long2 long2 long2
long2 long2 long2 long2 long2
91 91 1 2 3 4 5 6 7 8` 9 10 11 92 12 13 14 15 16 17 92 18 19 20 21 22 23 92 24 25 27 28 29 93 30 31 32 33 34 35 93 36 37 38 39 40 41 93
42 43 44 45 46 47 94 48 49 50 51
10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 Standard 10 Standard 10 Standard 10 Standard 10 Standard 10 Standard 10 Standard 10 Standard 10 Standard 10 Analyte 10 Analyte . . 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 QC 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 QC 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 QC 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 QC 10" Analyte 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 QC 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 Analyte ' 10 QC 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 Analyte
.
1 '
C
M asslynx - Sam ple List
Sam ple List: C:\MASSLYNX\FACT-1.PRO\Sam pleDB\022399c.SPL
Printed: T u e Feb 23 16:25:20 1999
c
5ni^
lP.lMa _____ti tt*C Q- .S. A/IqPIa La
l
,
F ile Nam e File Text
M S File Inlet I Botti olum e Sam ple T
60 022399079 Tox 120 DNO. 134F-G 3,2/22/99
TOX12C long2 52
61 022399080 Tox 120, RBL02229-250ppb-CC7, 2 / TOX12C long2 53
62 022399081 MeOH Blank
TOX12C long2 94
63 022399082 Tox 120 DNO. 135F-G 3,2/22/99
TOX12C long2 54
64 022399083 Tox 120 DNO. 136F-G 3,2/22/99
TOX12C long2 55
65 022399084 T o x 120 DNO. 137F-G 3,2/22/99
TOX12C long2 56
66 022399085 Tox 120 DNO. 138F-G3, 2/22/99
TOX12C long2 57
67 022399086 Tox 120 DNO. 139F-G 3,2/22/99
TOX12C long2 58
68 022399087 Tox 120, RBL02229-250ppb-CC8, 2/ TOX12C long2 59
69 022399088 MeOH Blank '
TOX12C long2 95
7 0 022399089 Tox 120 DNO. 141F -G 3 ,2/22/99 . TOX12C long2 60
71 022399090 Tox 120 DNO. 1 42F -G 3,2/22/99
TOX12C long2 61
72 022399091 Tox 120 DNO. 1 43F -G 3,2/22/99
TOX12C long2 62
7 3 022399092 Tox 120, RBL02229-250ppb-CC9, 21 TOX12C long2 63
74 022399093 MeOH Blank
TOX12C long2 95
75 022399094 Tox 1 2 0 ,1 06F-G1-250ppb-MS, 2/22/ TOX12C long2 64
76 022399095 Tox 1 2 0 ,1 06F-G 1-250ppb-M SD, 2/2 TOX12C long2 65
7 7 022399096 Tox 120, RBL02229-250ppb-CC10, TOX12C long2 66
78 022399097 MeOH Blank
TOX12C long2 95
79 022399098 Tox 120, RBL02229-H2O B lk-2,2/22 TOX12C long2 67
80 022399099 Tox 120, RBL02229-RBUverBlk-1, a TOX12C long2 68
81 022399100 Tox 120, RBL02229-5ppb-2,2/22/99 TOX12C long2 69
82 022399101 Tox 120, RBL02229-1 Oppb-2, 2/22/9 TOX12C long2 70
83 022399102 Tox 120, RBL02229-25ppb-2,2/22/9 TOX12C long2 71
84 022399103 Tox 120, RBL02229-50ppb-2,2/22/9 TOX12C long2 72
85 022399104 Tox 120, RBL02229-100ppb-2,2 /22/ TOX12C long2 73
86 022399105 Tox 120, RBL02229-250ppb-2,2 /22/ TOX12C long2 74
87 022399106 Tox 120, RBL02229-500ppb-2, 21221 TOX12C long2 75
88 022399107 Tox 120, RBL02229-750ppb-2, 21221 TOX12C long2 76
89 022399108 Tox 120, RBL02229-1000ppb-2, 2/22 TOX12C long2 77
10 Analyte 10 QC 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 QC 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 QC 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 Standard 10 Standard 10 Standard 10 Standard 10 Standard 10 Standard 10 Standard 10 Standard 10 Standard
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Page 2
Cone C (
1 Method Report
Method filai U l C odi fiad i
Cl \HASSLYNX\FACT-1 .9RO\HethI\022199c .edb Bai Feb 29 13125114 1999
] C-pOUHdl Printed!
2t prat
Med Her 03 lSt41i29 1999
C a p o u n d Me-- t
VPMS
Internal Ref.i
(None)
Quantify Tracei
TIC
1 Cone, of Standards!
Cone. A
User RP Valuet User Peak Factori
11..000000000000. Dieabled
Integrate Window Faeton
2.000
I Retention Ti-- (nine) t . Relative Retention Ti-- 1
4.5C3. enabled 0.000. Disabled
Ti-- Window (nine) t
1.117
Peak Selection!
Nearest
1 Reaponaa Typet Response 1
External . Areas
]
]
I
Polynomial Type* Point of teigini Fit Weighting! Axie Tranafoc-- tleni
Units1
.
Peak-to-peak amplitudet
--00thing! Window else (aeons) 1
Kueber of s-- othsi t-- o thing -- thodi
Linear Exclude NoNona
1*5
Disabled 1 2
*
Join valley threshold]. Reduce peak tailing] Raise baseline -- x i e u n
30.00 70.00 4.00
Drew vertical threshold! Deteet shouldersi Shoulder threshold!
10.00 Disabled 10.00
Relative height! Absolute height! Relative or-- ! Absolute area]
'
50.00. b a b i e d 0.00, Disabled 3.00. b a b i e d 0.00, Disabled
Soup p a o i q ^
BACK TO MAIN
Pag 2
u c , 0 3 /0 3/ ^ ^
Mathod Report Hathod filai Last aodiflads
Conpound Fr intads
Ct \KASSLYNX\PACT-1.PR0\MatbDR\022399c.ndb Thu Fab 25 13:25tl4 1999
3t FORA
Wad War 03 1Ci4 1 29 1999
Internal Raf.t Quantify Trae*i Cone, of Standardst
Vaar w Valuai Usar P M k Faeton Integrata Window Faeton
MU IWon*] TIC
11..000000000000. Diaablad
2 .0 0 0
Fataneion Tin* (niaa) i Salativ* Retention Tinas Tin* Miniar (mina) t Peak Salactioni
Raspansa Typai Responses
7.450. b i a M a d 0.000. Diaablad 0.902
External
Araas
Folynoadal Typai
Folnt of Origini Fit Weighting* Axis Tranafomation t
^ Uaitai
F*ak-to-p*ak aaplitudai
Sanothingi
Window also (scana)t Nunbar of aeothsi soothing aathodi
D1iaablad
2
Join vall*y tbrasholds Raduca paak tailings Raisa haslina aarinns
30.00 70.00 4.00
Draw vortical thraholdi Datact shoulders s Sbouldar thraaholdt
10.00 Diaablad 10.00
Ralativ* haighti Abaolut* heights Ralativ* araas Abaolut* araas
50.00. Cnablsd 0.00. Diaablad 3.00, Xaablod 0.00. Diaablad
_____ Soup
BACK TO MAIN
-.
;;
___lAiL_ OilW i l
Faga 3
Method Report
Method C i U l U i t aedifiodi
Cospoundt
Printed*
Ct\MASSLYMX\rACT-l.PRO\MethDS\022399e.mdb Thu Fob 25 13*25*14 1599
4 1 M513
Med Mor 03 14*41*29 1999
Ceeyound Nano* Internal Ref.* Quantify Trace* Cone, of Standards*
User RP Value* Veer Peak Factori Integrate Window Factor*
M513
(Mono) TIC Cone. A
.
11..000000000000, Disabled
2.000
Retention Tino Inins) t Relative Retention Tine* Tine Window (nine) i
teak Selection*
S.44S. b a b i e d 0.000, Disabled 0.730
Hearest
Response Type* Response*
3 Polynomial Type* Point of Origin* Pit Weighting* Axis Transformationi
External Areas
units*
1
Peak-to-peak amplitude*
Smoothing *
1 Window site (scans)* Humber of snooths* Smoothing method*
2
Join valley threshold* Reduce peak tailing* Raise baseline maxim*
Draw vertical threshold* Detect shoulders* Shoulder threshold*
30.00 70.00' 4.0Q
10.00 Disabled 10.00
Relative height* Absolute height* i Relative area* Absolute area*
50.00, b a bied 0.00, Disabled 3.00, b a b i e d . 0.00, Disabled
BACK TO MAIN
S o u p 2 6 M ______ H e
Ol/ollw
9*00 4
'-**. >
Hathod Rapert
Method filai Last nodifiad
Coapoundt
Frintedi
Ct\MAS5LYMt\FACT-l.PR0\KetkD\O22399e.db Thu Fab 23 13r25i14 1999
Si M<13
Wad Mar 03 Ki41 i 2 9 1999
Intanai Mf.i Quantify Tracci Cone. of Standard*!
Usar RF Valuai Vaar Feak Faeton Integrata Wlndow Faeton
M13
tuonai
TIC 1.000000. Diaablad
1.000000
2 .0 0 0
Rataation Tina (adna) i Relativa Rataation Tinai Tina Wlndow (nina) t
Faak Salaetioat
9.391, Knabled 0.000# Diaablod 0.730
1 Raapooaa Typai
Extamal Ara**
tolyaonial Typai Foint of Origini Fit Walghtingi Axia Trans f o n a tieni
Linear Include
1
fask-to-peak anplitudat
Swothingi Wlndow alca (aeana)t
1 ttanbar of anoothai fnnothlng nathodi
Join valla/ threaholdi Reduce peak tallingt Raiaa baseline aaxinuai
Draw vartieal thrasholdt ! Detect ahouldarai I Shouldar thrasholdt
i -------------
Relativa haighti Absoluta haighti Relativa arasi Absoluta arasi
Ditabiad
1
2
30.00 70.00 * 4.00
10.00 Diaablad 10.00
30.00, Enabled 0.00, Diaablad 3.00, Inablad 0.00, Disebi ad
BACK TO MAIN
-
/
So u p .,.D3o.tiq___ :___ (AL___ :0 3 /0 3 /9 '?
Faga S
Hatiio d Rap o rr
Method filo*
- C>\NAMLYKX\PACT-1.9ft0\MethDR\O22399e.wtb
Last aedi fiad*
Ttu Pb 25 13*25*14 1999
Ccepouadt
4t U
.
Printad*
Wd Kar 03 14*41*29 1999
CompoundMmi
In tern a i M ..I Q uan tify Trac* con e, o f Standard*
Uaar RP Valu* Uar Paak Pactor* Intgrt Hindou Pactor*
Is
ne(No m ]
Cne. B
1.000000, Disabled 1.000000 2.000
Rtention Tina (aina)* . Relative Rotention Tina* Tina Hindou (aina) *
Paak Sletlent
7.410, Enabled 0.000, Disabled 0.94
Nearest
Rasponaa Type* Raapons*
External . Areas
Pelynaalal Type* Paint ef Origint Fit Meightiag*
Axis Transformation*
Linear Exclude
None No m
.
*
unita* Feak-to-peak asplitude*
its
-
Metbing* Hindou sire (scana)i Nuaber of maotha tanolrhing nathod*
..
Disabled
1 2
Joln va11y threahold* Rduc paak taillagi Rai baseline maximum*
`
30.00 70.00 4.00
Draw vertical threahold* Datact ahouldarai Shoulder thresbold*
10.00 Disabled 10.00
Ralativ baight* Abaelut baight* Ralativ arat Abaelut ar*a*
*
50.00, Inabled 0.00, Disabled 3.00, Enabled 0.00, Disabled
.
_______ Soup
.
.
'
BACK TO MAIN
hg c
j}
lAL Oli*/m ____
FACT: DATA REVIEW SUMMARY FORM
BACK TO MAIN
Date:
Analyst:
zie;
FACTStudyNumber / OY. /<0
Analyte 4:
/yi S /3
Acceptable LinearRange: 573 - 7 5~o
Matrix
Time Points
Dilutions?
3 Analyte 1:
1 AcceptableLinearRange: 5 - S & 0 IaT* h
LOQforthis dataset -S'
C C '5 r
PASS
?
Re-evaluatedfar different range?
Flht r\ties ft
cliiuW\ lAl
LOQforthis dataset:
CO\'
"
tsUtA k,rRe-evaluatedfordifferentrange?
DiluW
y p 3 ftfoaiJqy?
......
0/* S b U d i \ ^ .
AnalyteS:
/r? 6 / 3
AcceptableLinearRange: ~5?>Q
LOQforthis dataset:
CC '>; f r z s
^
Re-evaluatedfordifferentrange? _______
Analyte 2: AcceptableLinearRange: 5~~ 5 & /^oL
Analyte 6:
-=zs
677*- OSfVsVM/c- / Y ^ o H i - A ^ j O / Z & i
AcceptableLinearRange^
'
LOQforthis dataset a??5 / _____
CC !s r /yioi-r
''
LOQforIbis dataset: Re-evaluatedfordifferentrange?
J V t & f /fccZ*T7<)4C`--
l -- c ^ w e /vor
/ & 'ffYT'*
Analyte3:
PoAA
Analyte 7:
3 AcceptableLinearRange: /O --SV&jQfb
Acceptable LinearRange:
LOQforthis dataset:
CC%:
'
Re-evaluateafor different range?
LOQfards dataset: Re-evaluatedfordifferentrange?
fctole.* R e a n a l g i ^ -G>r PF|)$ Q f i A d J u i l i O n i I`.S'
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AM MA1 MM1 I4IMMI0IMMMMOMAM
lini l i m i i n n i miiij
'!
BACK TO MAIN
Quantify Confound Sunnary Raport Tox 120 DNO, Jlat Livar Extracts
Saaple H a t : C:\KASSLTOX\FACT-l.PBO\8a^pleDB\022399c Laat nodiflad: Thu Tab 23 13:24:27 1999
Method:
C:\HAaSLXHX\FACT-l.PRO\HethDB\022399c.
L u t nodi lad: Thu Fab 25 13:25:14 1999
job Coda:
Printad:
Thu Fab 25 13:43:49 1999
'
Confound 2: PFBfl
Kama 1 022399020 2 022399021
3 022399022 4 022399023 5 022399024
6 022399025 7 022399026 8 022399027 9 022399028 10 022399029
11 022399030 12 022399031 13 022399032
14 022399033 15 022399034 16 022399035 17 022399036 18 022399037 19 022399038 20 022399039 21 022399040 22 022399041 23 022399042 24 022399043 25 022399044 26 022399045 27 022399046 28 022399047 29 022399048 30 022399049 31 022399050 32 022399051 33 022399052 34 022399053 35 022399054 36 022399055
37 022399056 38 022399057 39 022399058
40 022399059 41 022399060 42 022399061 43 022399062 44 022399063 45 022399064 46 022399065 47 022399066 48 022399067
49 022399068 50 022399069 51 022399070 52 022399071 53 022399072 54 022399073
55 022399074 56 022399075 57 022399076 58 022399077
59 022399078 60 022399079 61 022399080 62 022399081 63 022399082 64 022399083 65 022399084 66 022399085 67 022399086 68 022399087 69 022399066 70 022399089 .71 022399090
72 022399091 73 022399092 74 022399093
75 022399094 76 022399095 77 022399096
78 022399097 79 022399098 80 022399099
81 022399100 82 022399101 83 022399102 84 022399103 85 022399104 86 022399105 87 022399106 88 022399107
89 022399108
Saaplalist Text
MeOH Blank
MaOH Blank
Tox 120, RBL02229-H2O Blk-1, 2/22/99
Tox 120. RBL02229-RBLivarBlk-l. 2/22/99
Tox 120, RBL02229-5ppb-l, 2/22/99
Tox 120, RBL02229-10ppb-l, 2/22/99
Tox 120. RBM2229-25ppb-l, 2/22/99
Tox 120, R8L02229-50ppb-l, 2/22/99
Tox 120, RBL02229-100ppb-l, 2/22/99
Tox 120, RBU>2229-250ppb-l, 2/22/99
Tox 120, RBL02229-500ppb-l, 2/22/99
Tox 120, RBL02229-750ppb-l, 2/22/99
Tox 120, RBL02229-1000ppb-l, 2/22/99
MeOH Blank
TOX 120 DNO. 97F-G1. 2/22/99
TOX 120 DNO. 98F-G1, 2/22/99
Tox 120 DUO. 99P-G1. 2/22/99
Tox 120 DUO. 10F-C1, 2/22/99
Tox 120 DMO. 10IF-Cl, 2/22/99
Tox 120, RBL02229-250ppb-CCl, 2/22/99
MeOH Blank
Tox 120 DNO. 102F-G1, 2/22/99
Tox 120 DMO. 103F-G1. 2/22/99
Tox 120 DNO. 104F-G1, 2/22/99
Tox 120 DNO. 105F-C1, 2/22/99
Tox 120 DNO. 106F-G1, 2/22/99
Tox 120, RBX,02229-250ppb-CC2, 2/22/99
MeOH Blank
Tox 120 DNO. 107F-G1, 2/22/99
Tox 120 DNO. 109F-G1,.2/22/99
Tox 120 DNO. 110F-G1, 2/22/99
Tox 120 DNO. 11F-G1, 2/22/99
Tox 120, RBL02229-250ppb-CC3, 2/22/99
MeOH Blank
'
T ox 120 DNO. 113F-G2, 2/22/99
Tox 120 DNO. 114F-G2, 2/22/99
Tox 120 DNO. 115F-G2, 2/22/99
Tox 120 ONO. 116F-G2. 2/22/99
TOX 120 DNO. 117F-G2. 2/22/99
Tox 120, RBL02229-250ppb-CC4. 2/22/99
MaOH Blank
Tox 120 DNO. 118F-C2. 2/22/99
Tox 120 DNO, 119P-G2, 2/22/99
Tox 120 DNO. 120F-G2, 2/22/99
Tox 120 DNO. 121F-G2. 2/22/99
TOX 120 DNO. 122F-Q2, 2/22/99
Tox 120, RBL02229-2S0ppb-CC5, 2/22/99
MaOH Blank
Tox 120 DNO. 123F-G2, 2/22/99
Tox 120 DNO. 125F-G2, 2/22/99
Tox 120 DNO. 126F-G2, 2/22/99
Tox 120 DNO. 127F-G2. 2/22/99
Tox 120 DNO. 129F-G3, 2/22/99 TOX 120, RBL02229-250ppb~CC6, 2/22/99
MaOH Blank
Tox 120 DUO. 130P-C3, 2/22/99
TOX 120 DNO. 131F-G3, 2/22/99
Tox 120 DNO. 132F-G3, 2/22/99
'Tox 120 DNO. 133F-G3, 2/22/99
Tox 120 DNO. 134F-G3, 2/22/99
Tox 120, RBL02229-250ppb-CC7, 2/22/99
MeOH Blank
.
Tox 120 DNO. 13SF-G3, 2/22/99
Tox 120 DtyO. 136P-C3, 2/22/99
'Tox 120 DNO. 137F-C3, 2/22/99
Tox 120 DNO. 138F-G3, 2/22/99
Tox 120 DNO. 139F-C3, 2/22/99
Tox 120, *BL02229-250ppb-CC8, 2/22/99
MeOH Blank
Tox 120 DNO. 141P-G3, 2/22/99
Tox 120 DNO. 142F-G3, 2/22/99
Tox 120 DNO. 143F-G3, 2/22/99
Tox 120, *BL02229-250ppb-CC9, 2/22/99
MaOH Blank
Tox 120, 106F-Gl-250ppb-KS, 2/22/99
Tox 120, 106F-Gl-250ppb-MSD, 2/22/99
Tox 120, RBL02229-250ppb-CC10, 2/22/99
MaOH Blank
Tox 120, RBL02229-H2O Blk-2, 2/22/99
Tox 120, RBL02229-RBLiverBlk-l, 2/22/99
Tox 120, RBL02229-5ppb-2, 2/22/99
Tox 120, RBl02229-10ppb-2, 2/22/99
Tox 120, RBL02229-25ppb-2, 2/22/99
Tox 120, XfiL02229-50ppb-2, 2/22/99
Tox 120, RBL02229-100ppb-2, 2/22/99
Tax 120, R8L0229-2SOppb>2, 2/22/
Tox 120, RBL02229-500ppb-2, 2/22/99
Tox 120, RBL02229-7S0ppb-2, 2/22/99
Tox 120, RBLO2229-000ppb-2, 2/22/99
Sag 2
Type Analyte Analyte Analyte Analyte Standard Standard Standard Standard Standard Standard
Standard Standard Standard Analyte
Analyte Analyte Analyte Analyte Analyte QC Analyte Analyte Analyte Analyte Analyte Analyte
QC Analyte Analyte Analyte Analyte Analyte
QC Analyte Analyte Analyte Analyte Analyte
Analyte
QC Analyte Analyte Analyte Analyte Analyte Analyte
QC Analyte Analyte Analyte Analyte
Analyte Analyte
QC Analyte
Analyte Analyte
Analyte Analyte
Analyte
QC Analyte
Analyte Analyte
Analyte Analyte
Analyte QC Analyte Analyte Analyte Analyte
QC Analyte Analyte Analyte
QC Analyte Analyte Analyte Standard
Standard Standard Standard Standard Standard Standard Standard
Standard
` '
. .
Std Cono
XT
5.00 10.00
25.00
50.00 100.00 250.00 500.00 750.00
1000.00
6.119 6.121 6.812 6.819 6.818 6.820 6.734 6.120 6-735 4.117
6.905
250.00
7.595 6.644
6.651
250.00
7.594 6.650
6.565
250.00 6.651 7.507
250.00 250.00
6.565
6.735 7.596
6.652
6.559
250.00 6.651
250.00
6.649 6.651
.6^564
7.419
250.00 250.00 250.00
6.564 6.565
7.597 7.505
6.564
.650 .455 6.455
5.00
10.00 25.00 50.00 100.00 250.00 500.00
750.00 1000.00
6.639
6.565 6.563 6.564 6.561 6.565 6.562 6.560 6.614
Area cone. %Dev Flags
240 67 301 545 1014 2165 5727 10773 15322 20746
254
3.60 24.13
6.46 17.91
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Quantify Calibration Report ton 120 OHO Hat Liver Sxtraeta
Calibration* C:\MM8LYia\rACT-l.FllO\CumDa\022399a
Last aodifiadx Thu F*b 23 13:43x28 1999
printed!
Thu Fab 25 13x43x55 1999
.
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1 .
Soup 020199xx qpantltated and printed by nee 2/25/99
Quantify Calibration Haport V o x 130 0N0# Sat Livar Xxtracta
Calibration! C:\XMSLntX\FACT-l.P1t0\CurTaO9\022399o
L u t nodiflad: Thu Tab 25 13:43:29 1999
Print ad:
Thu Fab 25 13:43:55 1999
1
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1
1
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1
Soup 020199it Quantitatad and printad by naa 2/25/99
'
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. pAffa 2
1 ___________________ _
I Quantily Calibrati xaport TO* 120 W O , Rat L l n r Kxtracta
Calibr a t i m i ci \M99una\ncr-l .mo\CarraBB\022399e
l u t nsdlflads Thu rb 25 13:43:2t 1995
rrlntad)
Thu Tmh 35 13:41:55 1999
]
1
]
1 1
j
J
3
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I
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I
Soup 020199: x qu*ntltfttd mn4 prlnttd b y m 2/25/99
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Quantify Calibrati* Haport Tox 120 OMO, Hat Librar Extracta
Cai ibrat ioni C:\NA5flLTn\PaCT-l.PlO\CamDB\022399o
U a t aodifiadi Thu Pab 25 13:43*20 1999
Prlntad:
Tfau Pab 25 13:43:55 1999
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Papa 4 ,
Soup 020199:: Quantitatad and prlntad by aaa 2/25/99
Quaatify Calibrati Itaport To 120 XMO# ftat Livar Xxtraeta
.
C a l i b r a t i m i C:\KiMLinoc\FACT-l.Fio\CarraDS\032399o
Last aodlfiadi Ttou Fato 25 13143:2* 1999
Frlatodi
ni Fb 29 13141153 1919
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Quantlfy Calibrati lapert m 120 EMO Hat Lirar Extracts
Calibratimi ci\maunoc\nc*>l.mo\ciirran\022399e
L u t aodifladi m fab 25 13t43i3* 1*
rrintadt
m u rab 25 13i<3iS3 1*
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oup 020X99H uaatltatad and prlafead by
2/25/99
Method Report
Method file* Last modified*
Compound
Printed
C \MASSLYHX\FACT-1. VftO\MethDS\022399c.mdb ttm Feb 33 133514 199* 1 pros
Wed Kar 03 16i4129 1999
Incarnai M t . i Quantify Tracai Cone, of Standard*i
Uaar *F Valuai Usar Peek Faeton Intagrato Window Faeton
[Nona] TIC
11..000000000000, Disabled
3.000
Hatanti on Tina (nina)i Kolativa Retention Tlnat Tin* Window (mina) i
Peak Selectioni
ft.03. enabled 0.000, Dieablad 1.030
Response Type
Polynomial Type
3 Point of Origin Fit Weighting:
Axis Transformationi
1 Peak-to-peak amplitude
Smoothingi Window site (scans) H u b e r of smooths Smoothing method
D1isabled a
Join valley threshold Reduce peak tailing Raise baseline maximum
Draw vertical threshold Detect shoulderst Shoulder threshold
30.00 70.00 4.00
10.00 '
Disabled 10.00
Relative height Absolute height Relative area Absolut* area
30.00, Enabled 0.00. Disabled 3.00, b a b i e d 0.00. Disabled
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i;
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Page 1
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SOUP 020199: FACT Equipment Log 99014
Quattro II,#6414E
PAGE
DATE: 2/24/99
..
ANALYST: drb
.
]
STUDY NUMBER:Tox-120 MATRIX: R at Liver DESCRIPTION: Liver Samples .
] FACTM ethod: FACT-M-4.0 .
.
Injection volume: 10 uL
Cycle tim e: 13.5 min.
Electrode: Z-Sprav_______
J M obile Phase:
(A12.0 mM NH4CH3CQ2
. (BVMeOH______________
TN-A- or stndxd number (A) 98012-1-4
ffi) TN-A-2260
1 Prepared (date/by):
/A) 2/11/99 hoi___
(E l 2/99. hoi
Expires:
(A) 8/99
(BV 8/99_______________ .
Stoip at room temp? |5] or N
O th er___________________ ;_____
1 G uard colum n: None________________ __
P art num ber na______ _
Lot number: n
a ____________
1 A nalytical colum n: Keystone Betasil__________________________________:____
I Size: 2 x 100 mm_______________ Type: C18____________ Sphere size: 5 um_________
Jt ,
'.
J P art number 105-701-2__________ Lot number: P8C09
Serial num ber 10881106D
A nalytical param eters:
.
Drying gas (L/hrl: 300
ESI nebulizing gas (L/hrl 15
Sheath gas fL/hrl 0
J LC Flow rate: 300 uL/min. Backpressure: 120 bar
'
H PLC solvent gradient: Long 2
Time fm in.l
JbB________
1)JLQQ___ ___
m Q _________
!
2) 8.50
90.0
!
3) 11.0
J(LQ__________
.
4) 12.0_________
40.0 ________
5) 13.5 . Stop__________
I
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Tuning Method Report
Method :
C :\MAS SLYNX \FACT -1. PRO\ACQUDB\PFOS
Printed:
Wed Feb 24 15:22:59 1999
Spuj> Q 1 0 W
IA<L 05/to f o
Page 1
1
3
1
I
!
{ MS
I
SOURCE ( ESP- )
j Capillary .
Cone
. Extractor
I RF Lens
J Source Block Temp.
Desolvation Temp.
]
j Pressures
.
J Analyser Vacuum Gas Cell
Set Rdbk
3.00 60
5 0.2 150 250
2.99 -60 -7
.150 250
Rdbk
1.2e-4 5.2e-3
MSI .
LM Resolution
HM Resolution
Ion Energy
Ion Energy Ramp
' Lens 6
Multiplier .
" .*v
MS2
LM' Resolution
HM Resolution
Collision.
Ion Energy
Ion Energy Ramp
Lens 8
.
Lens 9
Multiplier
Set Rdbk
14.0 14.0, 2.0 q . 0.0 53 650
Set Rdbk
15.0
15.0
45 42
2.0
0.0
40 38
.0
42
650
Solvent Delay ( nina ) I
0.00
Function t 1
MEM of 3 Mass Fairs ( W - )
-
Inter Channel Delay ( Secs ) *
Span { Daltons ) t ' Start Tie ( Mins ) t End Tine ( Mins ) i Repeats *
Channel Parent Daughter
0.03
0.00 0.00 13.50 1 Dwell (Secs) Coll Energy (eV)
Cone ( V.)
399.00 80.00 399.00 99.00 399.00 130.00
0.25 0.25
0.25
45 45 45
60 60 60
Function i 2
Iof 3 Mass pairs (M i - )
3 Inter Channel Delay ( Secs ) t Span ( Daltons ) > Start Tine { Mins ) Cnd Tine ( Mins ) t
0.03 0.00
0.00 13.50
*
*
Repeats i
1.
Channel Parent Daughter Dwell (Secs) Coll Knergy (eV) Cone ( V )
499.00 80.00
0.25
45
60
1 499.00 99.00 499.00 130.00
0.25 0.25
45 45
60 60
Function i 3
MRX of 2 Mass Pairs ( M l - )
1 Inter Channel Delay ( Secs ) i 0.03
Span ( Daltons ) i
0.00
Start Tine ( Kins ) *
0.00
.
End Tine ( Kins ) .i
13.50
Repeats i
1
Channel Parent Daughter Dwell (Secs) Coll Energy (eV) Cone ( V )
299.00 80.00 299.00 99.00
0 .2& 0.25
45 45
35 35
Function i 4
MEM of 3 Mass Pairs ( ESP- )
Inter Channel Delay ( Secs } i Span ( Daltons ) t
Start Tine ( Mins ) t * End Tine ( Kins ) s Repeats t
Channel Parent Daughter
0.03 0.0Q
0.00 13.50 1 Dwell (Secs) Coll Energy (eV)
. Cone ( V )
413.00 119.00 413.00 169.00 413.00 219.00
0.25 0.25
0.25
30 20 30
15 15
15
J Function i 5
MEM of 3 Mass Pairs ( ESP- )
Inter Channel Delay ( Sees ) s 0.03
Span ( Daltons ) i
1 0.00
Start Tina ( Mina ) t
* 0.00
End Tins ( Mina ) t
13.50
I Repasts i
1.
Channe l Parent Daughter Dwell (Secs) Coll Energy (eV) Cone ( V )
469.00 169.00 0.25
45
30
469.00 219.00 0.25
45
30
469.00 119.00 0.25
45
30
Function t 6
MSM of 3 Mass Fairs ( SSF- )
later Channel Delay ( Secs ) *
Span ( Daltons ) t
Start Tine ( Mins ) i '
End Tine ( Mina ) j
Repeats t
*
Channel Parent Daughter
569.00 169.00 569.00 219.00 569.00 119.00
0.03 0.00 0.00 13.50 1 Dwell (Seca) Coll Energy (eV)
0.25
0.25 0.25
45 45
45
Cone ( V )
35
35 35
Function t ?
MEM of 1 Mass Fair ( ESP- )
Zntsr Channel Delay ( Secs ) t 0.03
Span ( Daltons ) j
0.00
*
Start Tins ( Kins ) t End Tine ( Kins ) *
0.00 13.50
.
Repeats t
*1
Channel Parent Daughter Dwell (Secs) Coll Energy (eV) Cone ( V )
427.00 80.00
0.25
40
60 .
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M asslynx - Sam ple List
Sam ple List: C:\MASSLYNX\FACT-1 .PRO \Sam pleDB\022499a.SPL
Printed: W ed Feb 24 15:25:391999
,,
. /, I j .
_____________________________ W p m o m ___ lAC.QS/lolri,
0U >. co
'
Page 1
F ile Nam e File Text
M S File In le tl Botti olum e Sam ple T j C one A C o n e B C o n c C
1 022499001 MeOH Blank
TOX12C long2 91
10 Analyte
2 022499002 MeOH Blank
TOX12C long2 91
10 Analyte
3 022499003 Tox 120, RBL02229-H2O B lk -1 ,2/22 TOX12C long2 1
io Analyte
4 022499004 Tox 120, RBL02229-RBUverBlk-1, 2. TOX12C long2 2
10 Analyte
5 022499005 Tox 120, R BL02229-5ppb-1,2/22/99 TOX12C long2 3
10 Standard
'
6 022499006 Tox 120, RBL02229-1 Oppb-1,2 /2 2 /9 TOX12C long2 4
10 Standard
7 022499007 Tox 120, RBL02229-25ppb-1,2 /2 2 /9 TOX12C long2 5
. 10 Standard
8 022499008 Tox 120, RBL02229-50ppb-1,2/22/9 TOX12C long2 6
10 Standard
9 022499009 Tox 120, RBL02229-100ppb-1,2 /2 2 / TOX12C long2 7
10 Standard
10 022499010 Tox 120, RBL02229-250ppb-1,2 /2 2 / TOX12C long2 8 '
10 Standard
.
11 022499011 Tox 120, RBL02229-500ppb-1,2 /22 / TOX12C long2 9
10 Standard
12 022499012 Tox 120, RBL02229-750ppb-1.2 /22 / TOX12C long2 10
10 Standard
13 022499013 Tox 120, RBL02229-1000ppb-1,2 /2 2 TOX12C long2 11
10 Standard
14 022499014 MeOH Blank
.
TOX12C long2 92
10 Analyte
15 022499015 Tox 120 DNO. 145F-G 4,2/22/99 ` TOX12C long2 12
10 Analyte . -
16 022499016 Tox 120 DNO. 146F-G 4,2/22/99
TOX12C long2 13
10 Analyte
17 022499017 Tox 120 DNO. 147F-G 4,2/22/99
TOX12C long2 14
10 Analyte
18 022499018 Tox 120 DNO. 148F-G 4,2/22/99
TOX12C long2 15
' 10 Analyte
19 022499019 Tox 120 DNO. 4 9 F -G 4 ,2/22/99
TOX12C long2 16
10 Analyte
20 022499020 Tox 120, RBL02229-250ppb-CC11, TOX12C long2 17
10 QC
21 022499021 MeOH Blank
TOX12C long2 92
10 Analyte
22 022499022 Tox 120 DNO. 150F-G 4,2/22/99
TOX12C long2 18
10 Analyte
23 022499023 Tox 120 DNO. 151F-G 4,2/22/99
TOX12C long2 19
10 Analyte
24 022499024 Tox 120 DNO. 152F-G 4,2/22/99
TOX12C long2 20
10 Analyte
25 022499025 Tox 120 DNO. 1 53F -G 4,2/22/99
TOX12C long2 21
10 Analyte
26 022499026 Tox 120 DNO. 1 54F -G 4,2/22/99
TOX12C long2 22
10 Analyte
27 022499027 Tox 120, RBL02229-250ppb-CC12, i TOX.12C long2 23
10 QC
28 02249928 MeOH Blank
TOX12C long2 92
10 Analyte
29 022499029 Tox 120 DNO. 155F-G 4,2/22/99
TOX12C long2 24
10 Analyte
30 022499030 Tox 120 DNO. 157F-G 4,2/22/99
TOX12C long2 25
10 Analyte
31 022499031 Tox 120 DNO. 158F-G 4,2/22/99
TOX12C long2 27
10 Analyte
32 022499032 Tox 120 DNO. 159F -G 4,2/22/99
TOX12C long2 28
10 Analyte
.
33 022499033 Tox 120, RBL02229-250ppb-CC13, TOX12C long2 29
10 QC
.
34 022499034 MeOH Blank
TOX12C long2 93
10 Analyte
35 022499035 Tox 120 DNO. 161F-G 5,2/22/99 36 022499036 T o x 120 D N O . 162F-G5, 2/22/99
TOX12C long2 30 T O X 1 2 C long2 31
10 Analyte 10 Analyte
37 022499037 Tox 120 DNO. 163F-G 5,2/22/99
TOX12C long2 32
10 Analyte
38 022499038 Tox 120 DNO. 164F-G 5,2/22/99
TOX12C long2 33
10 Analyte
39 022499039 Tox 120 DNO. 165F-G 5,2/22/99
TOX12C long2 34
10 Analyte
40 022499040 Tox 120, RBL02229-250ppb*CC14, i TOX12C long2 35
10. QC
41 022499041 MeOH Blank
TOX12C iong2 93
10 Analyte
42 022499042 Tox 120 DN. 166F-G 5,2/22/99
TOX12C long2 36
10 Analyte '
43 022499043 T o x `120 DNO. 167F-G 5,2/22/99
TOX12C long2 37
10 Analyte
44 022499044 Tox 120 DNO. 168F-G 5,2/22/99
TOX12C long2 38
10 Analyte
45 022499045 Tox 120 DNO. 16 9F -G 5,2/22/99
TOX12C long2 39
10 Analyte
46 022499046 Tox 120 DNO. 170F-G5 2/22/99
TOX12C long2 40
10 Analyte
47 022499047 Tox 120, RBL02229-250ppb*CC15, i TOX12C long2 41
10 QC . `
`48 022499048 MeOH Blank
TOX12C long2 93
10 Analyte
49 022499049 Tox 120 DNO. 171F-G5 2/22/99
TOX12C long2 42
10 Analyte
50 022499050 Tox 120 DNO. 173F-G5 2/22/99
TOX12C long2 43
10 Analyte
51 022499051 Tox 120 DNO. 174F-G5 2/22/99
TOX12C long2 44
10 Analyte
52 022499052 Tox 120 DNO. 175F-G5 2/22/99
TOX12C long2 45
10 Analyte
53 022499053 Tox 120 DNO. 177F-G6 2/22/99
TOX12C long2 46
10 Analyte
54 022499054 TOX 120, RBL02229-250ppb-CC16, i TOX12C long2 47
10 QC
55 022499055 MeOH Blank
TOX12C long2 94
10 Analyte
,
56 022499056 Tox 120 DNO. 178F-G6 2/22/99
TOX12C long2 48
10 Analyte
'
57 022499057 Tox 120 DNO. 179F-G6 2/22/99
TOX12C long2 49
10 Analyte
58 022499058 Tox 120 DNO. 180F-G6 2/22/99
TOX12C long2 50
10 Analyte
59 022499059 Tox 120 DNO. 181F-G6 2/22/99
TOX12C long2 51
10 Analyte
BACK TO MAIN
M asslynx Sam ple List
Sam ple List: C:\MASSLYNX\FACT-1.PRO\SampleDB\022499a.SPL
Printed: W ed F eb 2 415:25:391999 r
Soup 0 1 0 m .
. . _ laA
lA^aSllojlf
/ .
Page 2
File Nam e R ie Text
M S File In let I Botti olum e Sam ple T j C one A C one B C one C
60 022499060 Tox 120 DNO. 182F-G6 2/22/99
TOX12C long2 52
61 022499061 Tox 120, RBL02229-250ppb-CC17, TOX12C long2 53
62 022499062 MeOH Blank
TOX12C long2 94
63 022499063 Tox 120 DNO. 183F-G6 2/22/99
TOX12C long2 54
64 022499064 Tox 120 DNO. 84F -G 6 2/22/99
TOX12C long2 55
65 022499065 Tox 120 DNO. 185F-G6 2/22/99
TOX12C long2 56
66 022499066 Tox 120 DNO. 186F-G6 2/22/99
TOX12C long2 57
67 022499067 Tox 120 DNO. 187F-G6 2/22/99
TOX12C long2 58
68 022499068 Tox 120, RBL02229-250ppb-CC18, ! TOX12C long2 5 9 .
69 022499069 MeOH Blank
' TOX12C long2 95
7 0 022499070 Tox t2 0 DNO. 189F-G6 2/22/99
TOX12C long2 60
71 022499071 Tox 120 DNO. 190F-G6 2/22/99
TOX12C long2 61
72 022499072 Tox 120 DNO. 191F-G6 2/22/99 . TOX12C long2 62
7 3 022499073 Tox 120, RBL02229-250ppb-CC1,2 / TOX12C long2 63
7 4 022499074 MeOH Blank
TOX12C long2 95
7 5 022499075 Tox 1 2 0 ,110F-G1-250ppb-MS, 2/22/ TOX12C long2 64
76 022499076 Tox 1 2 0 ,1 10F-G1-250ppb-MSD, 2/2 TOX12C long2 65
7 7 022499077 Tox 120, RBL02229-250ppb-CC2,2/ TOX12C long2 66
78 022499078 MeOH Blank
TOX12C long2 95
79 022499079 Tox 120, RBL02229-H2O B lk-2,2/22 TOX12C long2 67
80 022499080 Tox 120, RBL02229-RBLiverBlk-1,2. TOX12C long2 68
81 022499081 Tox 120, RBL02229-5ppb-2,2/22/99 TOX12C long2 69
82 022499082 Tox 120, RBL02229-10ppb-2,2/22/9 TOX12C long2 70
83 022499083 Tax 120, RBL02229-25ppb-2,2/22/9 TOX12C long2 71
84 022499084 Tox 120, RBL02229-50ppb-2,2/22/9 TOX12C long2 72
8 5 022499085 Tox 120, RBL02229-100ppb-2,2/22/ TOX12C long2 73
86 022499086 Tox 120, RBL02229-250ppb-2,2/22/ TOX12C fong2 74
8 7 02249987 Tox 120, RBL02229-500ppb-2,2/22/ TOX12C long2 75
88 022499088 Tox 120, RBL02229-750ppb-2,2/22/ TOX12C long2 TO
SS 022499089 Tox 120, RBL02229-1000ppb-2,2/22 TOX12C long2 77
10 Analyte 10 QC 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 QC 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 QC 10 Analyte . 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 Analyte 1 0. Analyte 10 Standard 10 Standard 10 Standard 10 Standard 10 Standard 10 Standard 10 Standard 10 Standard 10 Standard
FACT: DATA REVIEW SUMMARY FORM
BACK TO MAIN
Date: c j c n h
Analyst:
,
FACT StudyNumber ^ J L \2 Q
Matrix
Z vSrJA
Time Points
yfgMjxi
\o
Dilutions?
(vJ____
Analyte 1:
Pfcs
Acceptable TornearRange:
'/ / c s^p p t)
LOQ for Ibis data set
Re-evaluated for differentrange?
A)
Analyte 4:
n5n
Acceptable Linear Rang e : / ^ 0 ~~/ ,
LOQ for this data set / ^ o
Re-evaluated for.different range?
Analyte 5: Acceptable LinearRan g e:/O -- ' LOQ for this data set 1 Re-evaluated for differentrange?
Anafyte2:
Acceptable Linear Range: u.rv> oU ^ U y f a i&k,
r + ^ 'C c T T c o r r * X ^ 3 r \ - s ^
LOQ for tf set
t o A f-'OjL*~v
Re-evaluated far different range? ________
Analyte 3:
roAA
Acceptable Linear Range:
LOQ for this data s e t c P -tS ^ a p ^
Re-evaluated for different range?
fOo
1 - :i
L___
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BACK TO MAIN
Quantify Coneound Susmary Mport TCX-120 Rat Livar
larnpli List: C: \HASSLTncc\7ACT-l.?RO\aleDl\Oa2499a
Last modified: W ad Mar 10 09:21:55 1*99
Method:
Ct \MAS8L7MX\7ACT-l.fltO\HatbDS\022499a
Last nodlfladx Tua Mar 09 13:53:43 1999
Job Coda:
Priatadi
W ad Mar 10 09:43:5 1999
Compound 2: * n i
Mama 1 022499001 2 022499002
3 022499003 4 022499004
5 022499005 022499006 7 022499007 8 022499008
9 022499009
10 022499010
11 022499011 12 022499012
13 022499013 14 022499014
15 022499015 16 022499016 17 022499017
18 022499018 19 022499019 20 022499020
21 022499021 22 022499022
23 022499023 24 022499024
25 022499025 26 022499026 27 022499027
28 022499028
29 022499029
30 022499030 31 022499031
32 022499032 33 022499033 34 022499034
35 022499035 36 022499036
37 022499037
38 022499038
39 022499039 40 022499040
41 022499041
42 022499042 43 022499043 44 022499044
45 022499045 46 022499046 47 022499047
48 022499048 49 022499049 50 022499050 51 022499051 52 022499052 53 022499053 54 022499054 55 022499055 56 022499056 57 022499057 58 022499058
59 022499059 60 022499060 61 022499061
62 022499062 63 022499063 64 022499064 65 022499065 66 022499066 67 022499067
68 022499068
69 022499069
70 022499070 71 022499071
7^ 022499072 73 022499073 74 022499074 75 022499075 76 022499076 77 022499077
78 022499078 79 022499079 80 022499080 81 022499081
82 022499082 83 022499083 84 022499084 85 022499085 86 022499086 87 022499087 88 022499088
89 022499089
Sai^lallst Taxt MeOK Blank
*
MaOH Blank
Tox 120 , RBL02229-H2O Blk-1. 2/22/99 Tox 120 . RBL02229-RBUverBlk-l, 2/22/99
Tox 120 , RBL02229-5ppb-l, 2/22/99
Tox 120 , R5L02229-10ppb-l, 2/22/99
Tox 120 . RBL02229-25ppb-l, 2/22/99
Tox 120 , RBL02229-50ppb-l, 2/22/99 Tox 120 , RBLQ2229-100ppb-l, 2/22/99 Tox 120 , RBL02229-250ppb-l, 2/22/99 Tox 120 , RBL02229-500ppb-l, 2/22/99 . Tox 120 , RBLO2229-750ppb-l, 2/22/99
Tox 120 . RBL02229-1000ppb-l, 2/22/99
MaOH Blank
Tox 120 DUO. 145P-G4. 2/22/99
.
Tox 120 DUO. 1467-64, 2/22/99
Tox 120 DUO. 1477-64, 2/22/99
Tox 120 DMO. 1487-64, 2/22/99 Tox 120 DMO. 1497-64, 2/22/99
Tox 120,, RBL02229-25Oppb-CCll, 2/22/99
MaOH Blank Tox 120 DMO. 1507-64, 2/22/99 Tox 120 DMO. 1517-64, 2/22/99 Tox 120 DMO. 1527-64, 2/22/99 Tox 120 DMO. 1537-64; 2/22/99 Tax 120 DNO. 1547-64, 2/22/99 Tox 120,r RBL02229-250ppb-CC12, 2/22/99
MaOH Blank
Tox 120 DMO. 1557-64, 2/22/99
T ox 120 DMO. 1577-64, 2/22/99 Tox 120 DNO. 1587-64, 2/22/99
Tox 120 DNO. 1597-64, 2/22/99
Tox 120, ABL02229-250ppb-CC13, 2/22/99
MaOH Blank
Tox 120 DNO. 1617-G5, 2/22/99 Tox 120 DNO. 1627-G5, 2/22/99
Tox 120 DNO. 1637-C5, 2/22/99 Tox 120 DNO. 1647-G5, 2/22/99
Tox 120 DNO. 1657-G5. 2/22/99
T ox 120, RBL02229-250ppb-CC14, 2/22/99
MaOH Blank
Tox 120 DNO. 1667-G5, 2/22/99
Tox 120 DNO. 1677-G5, 2/22/99
Tox 120 DNO. 1687-G5, 2/22/99
Tox 120 DNO. 1697-G5, 2/22/99
Tox 120 DNO. 1707-G5 2/22/99
Tox 120, RBL02229-250ppb-CC15, 2/22/99
MaOH Blank Tox 120 DNO. 1717-C5 2/22/99
Tox 120 DNO. 1737-GS 2/22/99
Tox 120 DNO. 174P-G5 2/22/99
Tox 120 DNO. 1757-G5 2/22/99
Tox 120 DMO. 1777-G6 2/22/99
Tox 120. RBL02229-250ppb-CC16, 2/22/99 MaOH Blank
Tox 120 DNO. 1787-G6 2/22/99 T ox 120 DNO. 179P-G6 2/22/99
Tox 120 DNO. 1807-G6 2/22/99
Tox 120 DNO. 1B17-G6 2/22/99
Tox 120 DNO. 1827-G6 2/22/99
*
Tox 120, RBL02229-2S0ppb-CC17, 2/22/99 MaOH Blank
Tox 120 DNQ. 1837-06 2/22/99
Tox 120 DNO. 1847-06 2/22/99
Tox 120 DNO. 1857-G6 2/22/99
Tox 120. DNO. 1867-G6 2/22/99
Tox 120 DNO. 1877-G6 2/22/99
Tox 120, RBL02229-250ppb-CC18. 2/22/99
MaOH Blank
Ton 120 ONO. 189P-C 2/22/99
Tox ,120 DUO. 1907-C4 2/22/99
Tox 120 DUO. 1917-G6 2/22/99
Tox 130, RBL0222-2S0ppb-CCl. 2/32/99
MaOH Blank
.
Tox 120, 110F-Cl-250ppb-MS, 2/22/99 Tox 120, 110r-Cl-2SOppb-MSS, 2/22/99 Tox 120, UL02229-2S0ppb-CC2, 2/22/99 MaOH Blank
Tox 120, RBL02229-H20 Blk-2, 2/22/99 Tox 120, RBL02229-R8LivarBlk-l, 2/22/99
Tox 120, RBL02229-5ppb-2, 2/22/99
Tox 120, RBL02229-10ppb-2, 2/22/99
Tox 120, RBL02229-25ppb-2. 2/22/99
Tox 120, RBL02229-50ppb-2. 2/22/99
Tox 120, RBL02229-100ppb-2, 2/22/99
Tox 120, RBL02229-2S0ppb-2, 2/22/99
Tox 120, RBL02229-S00ppb-2, 2/22/99
Tox 120, RBL02229-750ppb-2, 2/22/99
Tox 120, RBL02229-1000ppb-2, 2/22/99
*ypa Analyta Analyta
Analyta Analyta Standard Standard Standard Standard
Standard Standard Standard Standard
Standard Analyta Analyta Analyta Analyta Analyta Analyta QC Analyta Analyta Analyta Analyta Analyta Analyta QC Analyta Analyta Analyta Analyta Analyta
QC Analyta Analyta Analyte Analyta Analyta
Analyta QC Analyta
Analyta Analyta Analyta Analyta
Analyta QC Analyta Analyta Analyta Analyta Analyta Analyta QC Analyte Analyta Analyta
Analyta Analyta Analyta
QC Analyta
Analyta Analyta Analyta Analyta Analyta
QC Analyta Analyta-
Analyta Analyta
QC Analyta Analyta Analyta Analyta Analyta Analyta Analyta Standard
Standard Standard
Standard Standard Standard
Standard
Standard Standard
Std Cone
5.99 12.00 29.90 59.90 120.00 299.00 599.00 898.00 1198.00
299.00
299.00
299.00
299.00
299.00
299.00
299.00
299.00
299.00
5.99 12.00 29.90 59.90 120.00 299.00 599.00 898.00 1198.00
RV
6.648 6.583 6.563 6.562 6.528 6.565 6.539 6.457 6.474 6.465
6.565 7.321 6.464 6.377 6.450 6.465
6.583 6.409 6.389
6.387
6.407
6.542 6.458 6.474 6.476 6.496 6.448 6.490 6.551 6.480 6.459 6.442 6.474 6.451 6.533 6.559 6.441 6.480 6.470 6.448 6.477
6.537 6.461 6.472 .450 6.448 6.466
6.548 6.469
6.472 *
6.411
6.565 6.481 6.376
6.502 6.456 6.512 6.433 6.464 6.481 6.479 6.477 6.477
Araa Dl
Cono. 71aas XDmr
157 463 250 314 552 981 2548 4498 7283 8654
428 * 68
36 138 147 1692
173 52
105
1 0.82 MX 1 46.18 MX 1 14.54 MX 1 24.11 bb 1 59.47 bb 1 123.26 MX 1 356.08 MM 1 645.67 MX 1 1059.54 MM 1 1263.23 MX
1 41.08 MX 1 0.00 bb 1 0.00 bb 1 0.00 bb 1 0.00 MM 1 228.87 bb
1 3.11 M M 1 0.00 MM 1. 0.00 M M .
671 21
-19 -1 3 19 8 18 5
-23
1132 1 145.57 MM .
-51
C O 1'*'
1354
224055 201712 162554 141519 161586
1657 156
201189 136264 112102
1454 803
1291 109
2497 179 212 222
1435 1317
1670 1445 1256 1196 1402 1548
2042 1376
1 178.69 MM
1 33265.77 MM 1 29946.12 MM 1.24128.42 MM 1 21003.21 MM 1 23984.62 MM 1 223.64 MM 1 0.58 MM 1 29868.45 MM 1 20222.42 MM 1 16632.67 MM 1 193.43 MM 1 96.74 MM 1 169.19 MM 1 0.00 KM 1 348.43 MM 1 4.02 bb 1 8.91 bb 1 10.37 bb 1 190.59 MM 1 173.13 MM
1 225.59 MM 1. 192.01 MM 1 164.01 MM 1 155.12 KM 1 185.75 MM V ^ 207.50 MM
1 280.82 KM
1 181.83 bb
-40 \ \
\
-25
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J -43
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-31
1603
1 215.57 KM
-28
1290
1936 1004 1297.
1 169.12 MM 1 265.13 MX 1 126.59 MM 1 170.13 MM
-43
61
82 183 485 887 2050 3785 5715 7311
1 0.00 MX 1 0.00 MX 1 4.70 MM 1 49.55 MM 1 109.18 MX 1 281.95 MX 1 539.86 MX 1 826.54 MX 1 1063.(6 MM
-10O -100
-84 -17
-9 -6 -10 -8 -11
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Soup 020199: Quantitatad on 03/09/99 and prlntad on 03/10/99 by DRB
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BACK TO MAIN
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BACK TO MAIN
Quantify Compound l u m x y tuport TOX-120 tat Livur
c l * Llati C:\M&SSUnoc\MCT-l.mo\fcl.DI\0229S.
Last aodifiad: N ad Max 10 0S)21>S5 1090
Mathod:
Cl\MUSLK\raCT-l.no\MathI\022499a
b u t aodifiad: Tua Mar 09 13:53:43. 1999
Job Coda)
rrintad:
' N a d Mar 10 09)43:3 1999
.
1
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53 .VJ
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1
COacound ) XI
MM
aaplallst Taxt
Typ
Std Cono
1 022499001 KeOH Blank 2 022499002 IteOH Blank
Analyta Analyta
3 022499003 Tox 120. RBL02229-H20 Blk-1, 2/22/99
Analyta
5 4 022499004 Tox 120. RBL02229-RBLivar81k-l. 2/22/99 Analyta
022499005 Tox 120. RBL02229-5ppb-l, 2/22/99
Standard
494.00
6 022499006 Tox 120. RBL02229-10ppb-l, 2/22/99
Standard
494.00
7 022499007 Tox 120. RBL02229-25ppb-l. 2/22/99
Standard
494.00
0 022499008 Tox 120. RBL02229-50ppb-l, 2/22/99
Standard
494.00
022499009 Tox 120. RBL02229-100ppb-l, 2/22/99 10 022499010 Tox 120. RBL02229-250ppb-l, 2/22/99
Standard Standard
494.00 494.00
11 022499011 T ox 120,BBL02229-500ppb-l, 2/22/99
Standard
494.00
13 022499012 Tox 120. RBLQ2229-750ppb-l, 2/22/99
Standard
494.00
13 022499013 Tox 120. RBL02229-100Oppb-1 2/22/99
Standard
494.00
1 022499014 IS 022499015
IteOH Blank
t o x 120 rara. I45r-c4. 2 /2 2 / 9 9
Analyta Analyta
1 022499016 T ox 120 OHO. 1467-C4, 2/22/99 ' 17 022499017 T ox 120 DUO, 147F-G4. 2/22/99
Analyta Analyta
18 022499018 T OX 120 DNO. 1487-04, 2/22/99
Analyta
19 022499019 T OX 120 DUO. 1497-64. 2/22/99
Analyta
20 022499020 Tox 120. RBL02229-250ppb-CCll. 2/22/99 QC
494.00
21 022499021 MaOH Blank
'
Analyta
22 022499022 Tox 120 DNO. 1507-64, 2/22/99
Analyta
23 022499023 TOX 120 DNO. 1517-64. 2/22/99
Analyta
24 022499024 To x 12Q DNO. 1527-64. 2/22/99
Analyta
35 022499025 To x 120 DNO. 1537-64, 2/22/99
Analyta
2 022499026 To x 120 DNO. 1547-64, 2/22/99
Analyta
27 022499027 Tox 120, RBL02229-250ppb-CC12, 2/22/99 QC
494.00
20 022499028 MaOH Blank
Analyta
29 022499029 To x 120 DNO. 1557^64. 2/22/99
Analyta
30 022499030 T q x 120 DNO. 1577-64, 2/22/99
Analyta
31 022499031 Tox 120 DNO. 1587-64, 2/22/99
Analyta
33 022499032 Tox 120 DNO. 1597-64. 2/22/99
Analyta
33 022499033 Tox 120. RBL02229-250ppb-CC13, 2/22/99 QC
494.00
34 022499034 MaOH Blank
'
Analyta
35 022499035 To x 120 DNO. 1617-65, 2/22/99
Analyta
36 022499036 To x 120 DNO. 1627-65. 2/22/99
Analyta
37 022499037 Tox 120 DNO. 1637-65, 2/22/99
Analyta
38 022499038 TO X 120 DNO. 1647-65, 2/22/99
Analyta
39 022499039 Tox 120 DNO. 1657-65, 2/22/99
Analyta
40 022499040 Tox 120, RBL02229-250ppb-CC14, 2/22/99 QC
494.00
*1 022499041 MaOH Blank 42 022499042 T ox 120 DNO. 1667-65, 2/22/99
Analyta Analyta
43 022499043 Tox 120 DNO. 1677-65, 2/22/99
Analyta
44 022499044 Tox 120 DNO. 1687-65. 2/22/99
Analyta .
45 022499045 T ox 120 DNO. 1697-65, 2/22/99
Analyta .
46 022499046 Tox 120 DNO. 1707-65 2/22/99
Analyta
022499047 Tox 120, RBl*02229-250ppb-CC15, 2/22/99 QC
*494.00
40 022499048 MaOH Blank
Analyta
49 022499049 Tox 120 DNO. 1717-65 2/22/99
Analyta
50 022499050 TOX 120 DNO. 1737-65 2/22/99
Analyta
si 022499051 Tox 120 DNO. 1747-65 2/22/99
. Analyta
S2 022499052 Tox 120 DNO. 1757-65 2/22/99
Analyta
S3 022499053 To x 120 DNO. 1777-66 2/22/99
Analyta
54 022499054 Tox 120. RB*02229-250ppb--CC16, 2/22/99 QC
55 022499055 MaOH Blank
Analyta
494.00
56 022499056- TOX 120 DNO. 1787-66 2/22/99
Analyta
57 022499057 Tox 120 DNO: 1797-66 2/22/99
Analyta
SS 022499058 TOX 120 DNO. 1807-66 2/22/99
Analyta
SS 022499059 Tox 120 ONO. 1817-66 2/22/99
Analyta
60 022499060 T ox 120 DNO. 1827-66 2/22/99
Analyta
61 022499061 Tox 120, BBL02229-250ppb-CC17, 2/22/99 QC
494.00
63 022499062 MaOH Blank
Analyta
63 022499063 Tox 120 DNO. 1837-66 2/22/99
Analyta
**s
022499064 022499065
Tox 120 DNO. 1847-66 2/22/99 Tox 120 DNO.* 1857-66 2/22/99
'
Analyta Analyta
6 022499066 Tox 120 DNO. 1867-66 2/22/99
Analyta
67 022499067 Tox 120 DNO. 1877-66 2/22/99
Analyta
68 022499068 Tox 120, RBI.02229-250ppb-CC18, 2/22/99 QC
494.00
69 022499069 MaOH Blank
Analyta
70 022499070 TOX 120 DNO. 1897-66 2/22/99
Analyta
022499071 T ox 120 ONO. 1907-66 2/22/99
Analyta
92 022499072 To* 120 DNO. 1917-66 2/22/99
Analyta
'S 022499073 Tox 120, RBM2229-250ppb-CCl, 2/22/99
QC
494.00
74 022499074 MaOH Blank
Analyta
75 022499075 Tox 120, 1107-61-250ppb-MS. 2/22/99
Analyta
76 022499076 Tox 120, 1107-61-25Oppb-MSD, 2/22/99
Analyta
77 022499077 Tox 120, RBL02229-250ppb-CC2, 2/22/99 Analyta
7 022499078 MaOH Blank
Analyta
79 022499079 Tox 120, HBL02229-K2O Blk-2, 2/22/99
Analyta
0 022499080 Tox 120, RBL02229-RBLivorBlk-l, 2/22/99 Analyta
u1 022499081 Tox 120, RBL02229-Sppb-2, 2/22/99 022499082 Tox 120, RBLO2229-10ppb-2, 2/22/99
Standard Standard
494.00 494.00
03 022499083 T ox 120, RBL02229-25ppb-2, 2/22/99
Standard
494.00
as54 022499084 Tox 120, RBL02229-50ppb-2, 2/22/99 022499085 Tox 120, KBta02229-100ppb-2, 2/22/99
Standard Standard
494.00 494.00
06 022499086 Tox 120, KBU)223?-250ppb-2, 2/22/99
Standard
494.00
87 022499087 Tox 120, RBL02229-S00ppb-2, 2/22/99
Standard
494.00
88 022499088 Tox 120. RBD02229-750ppb-2, 2/22/99
Standard
494.00
89 022499089 Tox 120, RBU)2229-1000ppb-2, 2/22/99
Standard
494.00
X
7.314 7.320 7.252 7.236 7.235 7.194 7.237 7.209 7.122 7.145 7.138
7.237 7.136 7.050 7.049 7.116 7.138
7.163 7.082 7.062 7.142 7.031 7.060
7.212 7.142 7.130 7.054 7.072
7.209 7.131 7.144 7.063 7.160 7.119
7.223 7.068 7.130 7.106 7.057 7.124
7.231 7.112 7.151 7.138 7.035 7.145
7.211 7.134 7.059 .7.037 7.035 7.141
7.131 7.142 7.137 7.135 7.143 7.145
7.137 7.068 7.080 6.996
7.152 7.066 7.050
7.208 7.197 7.169 7.122 7.093 7.105 7.136 7.068 7.066 7.064 7.064
oup 020199) Quantitatad on 03/09/99 and pclntad on 03/10/99 by D U
A T M Dl .. Cono. Flaga %Dav
348 278 665 847 768 713 583 663 673 673 746
389 * 632
421 472 617 672
329 481 599 580 402 555
316 473 464 362 654
383 676 588 604 515 558
349 497 586 441 35 3 542
340 534 513 493 231 515
236 216 226 235 204 571
199 225
281 208 308 575
285 351 306 546
322 366 531
308 318 670 704 75 748 691 732 642 638 537
1 248.61 MM 1 198.55 bb 1 474.76 MM 1 604.74 MM 1 548.31 bb 1 509.24 bb 1 416.56 bb 1 473.22 bb 1 482.12 MM 1 480.59 bb 1 532.80 bb
1 278.17 MM 1 451.67 bb 1 300.56 bb 1 336.95 bb 1 441.04 bb 1 480.18 bb
1 235.15 bb 1 343.87 bb 1 428.10 bb 1 414.05 MM 1* 287.31 bb 1 396.73 bb
1 225.86 MM 1 337.66 MM 1 331.42 MM 1 258.72 bb 1 466.87 bb
1 273.25 MM 1 482.50 bb 1 420.31 bb 1 431.38 bb 1 367.61 bb 1 398.50 bb
1 249.01 bb 1 355.24 bb 1 418.91 MM 1 314.98 bb 1 252.29 MM 1 387.24 MM
1 242.98 MH 1' 381.17 bb 1 366.66 MM 1 352.10 MM 1 164.71 MH 1 367.80 bb
1 168.62 bb 1 154.58 bb 1 161.28 MM 1 167.67 bb 1 146.02 bb
\ 407.90 bb
1 142.43 bb 1 160.54 bb 1 200.47 MM 1 148.91 bb 1 220.14 bb 1 410.85 bb
1 203.31 bb 1 250.92 MM 1 218.67 bb 1 389.67 bb
1 229.80 bb 1 261.34 bb 1 379.16 bb
1 219.86 MM 1 226.93 MM 1 478.83 MM 1 502.64 bb 1 540.16 bb 1 533.98 bb 1 493.58 bb 1 523.07 MM 1 458.22 bb 1 455.91 bb 1 383.25 HM
-50 -60
-4 22 11
3 -1
-4 -2 -3
8
-44 -9
-39 -32 -11
-3
-52 -30 .-13 -16 -42 -20
-54 -32 -33 -48
-5
-45 -2
-15 -13 -26 -19
-50 -28 -15 -36 -49 -22
-51 -23 -2 -29 -67 -2
-66 -69 -67 -66 -70 -17
-71
-
-59 -70 -55 -17
-59 -49 -56 -21
-53 -47 -23
-55 -54
-3 2 3 8 0 6
-7 -8 -22
9ago
Quantify Calibration taport
, MX-130 Mat blaar
l
! Calibration C:\MXaiUna\FACi-l.WUJ\Curruia\033499a
] Laat nodlfiad: Wad Mar 10 09:42:03 1*99
rrlntad!
Mad Mar 10 09:44:01 199
BACK TO MAIN
Quantify Calibration lteport TGX-120 Hat U w
Calibration! C:\KXJSUnz\7iCT~l.no\CurraOS\032499a
Laat nodlfiedi Wad Mar 10 09 z42: OS 1999
Prlntadf
W ad War 10 09:44:01 1999
Compound 2 name: PFHS
Correlation coefficient: r = 0.989537, rA2 = 0.979184 . . Calibration curve: 6.73075 x + 151.787'
Response type: External Std, Area Curve type: Linear, Origin: Exclude, Weighting: Null, Axis trans: None
8.65e3n
BACK TO MAIN
*9 2
Soup 020199: Quantitated on 03/09/99 and printed on 03/10/99 by D M
Quantiiy Calibratioa Jlaport
xax-120 lut Livar
Calibrai ioni
Lue aodifladi
yrlntadl
C>MOM
ad Mar
Mad Mar
U110M0SX9S\i:rM44Cii O0T1S-l1.1n91o991\Camn\0224S*m
BACK TO MAIN
oup 02019! Quantltatad on 03/09/99 and prlntad ea 01/10/99 fcytu
Quantify Calibration Itaport vex-130 Hat Livar
Calibration* C:\MMILYKX\rACT-l.ntO\Currain\022499a
Last nodi Si a d t W a d Mar 10 09*43:05 1999
Print ad:
Ma d Mar 10 09*44*01 1999
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Boup 020199* Quaatitatad on 03/09/99 and printed on 03/10/99 by SUB
g u u t l t y Calibration Rapoct TOX-12Q Hat U n r
Cal i b r a t i m i Ci\MUautn\rA-l .MO\Cu x v a \ 0 2 2 4 1 1 a
bast aodiiiadt ad Mar 10 01:12:00 1111
rrlacadi
Wad Mar 10 02:44:01 1212
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loop 0201221 Qoantitatad oa 03/02/22 aad printad on 03/10/21 b y M
Quantify Calibratioa Raport S0K-130 Rat Livar
'
C a lib ra tim i C:\XUILniX\riCT-l.rao\CuirTaDa\0224fla
U s e aedlfiadi a d Mar IO 09)43)05 1999
yrintadl
Msd Mar 10 09)44)01 199
`
' .
BACK TO MAIN
rag | '
' 1 '
.
oup 030199) Quantltatad on 03/09/99 and printad on 03/10/99 by m
Method Report
Method files Last modified:
Compounds
Printed:
Cl\KASSLTOX\FACT-l.FRO\H.thDB\022i99.ndb
Fri Tmb 2< 10i3 0 i39 1)9*
'
Is PFOS
Tue Mar 09 13s22s01 1999
.
Compound Kama: Internal Ref.> Quantify Tracei Cone, of Standardsi
User W Value t User Peak Faeton Integrate Window Faeton
PFOS INone] TIC Cone. A
1.000000, Disabled
1.000000
2.0 0 0
--
Soup 0201^
Retention Tine (sins) i Relative Retention Tines Tine W indow (mins) t
Peak Selections
1.030, 0.000, Disabled
1.030
Response Types Responses
Internal Areas *
Polynomial Types Point of Origins Fit Weightings Axis Transformations
Linear exclude Hone Hone
Unites
Peak-to-peak amplitudes
185
Smoothings
Window size (scans)s Number of ssnooths: Smoothing methods
Disabled
1
2
Join valley thresholds Reduce peak tailings Raise baseline maxiaums
30.00 70.00 4.00
Draw vertical thresholds
Deteet shoulderss
Shoulder thresholds
.
10.00 Disabled 10.00
Relative heights Absolute heights Relative areas. Absolute areas
50.00, 0.00, Disabled 3.00, Enabled .0.00, Disabled
*
LAc 0 3 /09/99
BACK TO MAIN
Page 1
Method Report
| Method files Last modified!
1 Compounds
Printed!
CsVMASSLYMX\FACT-l.PRO\MethDB\022499a.mdb . Fri Feb 2( 10s30s39 1999
2s PFHS
Tue M ar 09 13s22s01 1999
I Compound Hasses | Internal Raf.s
Quantify Tracas Cone, of Standards!
Usar W Valas Usar Paale Factors Integrate Window Factors
FFHS
{Hone]
TIC
Cone. A
.
1.000000, Diaablad
1.000000
2 .0 0 0
Retention Time (mins) s Relativa Ratention Tinas
1 Tina Window (nina) s Peak Selectiont
.563, Knablad 0.000, Diaablad 1.117
Haarest ' *
] Response Types Responses
Polynomial types
] Point of Origins Fit Weightings Axis Transformations
Rxternal Areas
Linear Kxclude Hone Hone
Unitss
1
'
Peak-to-peak amplitudes !
18S
1 Smoothings Window .is. Ii c i d i ) i Humber of smoothes Smoothing methods
j
. Disabled
1 2
1 Join valley thresholds *
Reduce peak tailings Raise baseline maximum!
Y
30.00 70.00 4.00
1 Draw vartical thresholdt
Detect shoulderss
Shoulder thresholds
.*
10.00
Disabled 10.00
] Relative heights Absolute heights Relative araat Absolute areas
1
50.00, Rnabled , 0.00, Disabled
.00, t u b l a d 0.00, Disabled
3
BACK TO MAIN
lag. 2
5nu0F A2DI44 _LAC Qiloilw
J J
I
i
BACK TO MAIN
Method Report
Method filet Last modified!
Compound!
Printed t
C:\KASSLYNX\FACT-l.FRO\MethDB\022499a.mdb . Fri Feb 26 10t30t39 1999
3f POAA
Tue Mar 09 13i22i01 1999
.
Compound Hemet Internal Ref.i Quantify Traai. Cone, of Standards
Usar KT Valuat
Usar Peak Faeton
.
Integrata Window Faeton
POAA [Hone] TZC Cone. A
. .
1.000000, Disabled
1.000000
2 .0 0 0
Retention Time (nine) i Relative Retention Timet Time Window (mins) t
Peak Selection!
7.458, Xnabled 0.000, Disabled 0.902
Nearest '
Response Typet Response!
Internal Areas * ,
*
Polynomial Type* Point of Origini Fit Weighting! Axis TransformationI
t.in e a r Exclude
3(one
Hone
Unitsi
Peek-to-peek amplitudet
185 . . '
Smoothing! Window sise (scans)t Number of smooths: Smoothing method!
Join valley threshold!' Reduce peak tailing! Raise baseline maximum:
. Disabled 1 2
30.00 70.00 4.00
Draw vertical threshold!
Detect shoulders!
Shoulder threshold!
.*
10.00
Disabled 10.00
'
Relative height: Absolute height!
Ralativ. u n i ' Absolute areat
' 50.00, Enabled , 0.00, Disabled
3.00, f e a b l d 0.00, Disabled
' '
Soup nloiM __ M C o:lionlw
r
'
t*v
ps . ]
* ,
Method Report
Method file: Last modified:
Compound:
Printed:
Cl \MASSLYMX\ FACT-1. PRO\M.tM)B\022499A.Mdb . Fri Fall 2( 10(30.39 1999
4l MS 13
Tue Mar 09 13:22:01 1999
.
Compound Ni h i Internal Ref.: Quantify Trac: Cone, of Standarda:
Var R7 Vaiti i Uar Peak rector: Integrate Window Factor:
MS 13
(Ko m )
TIC Cone* A
.
1.000000, Disabled
1.000000
2.000
Retention Tin (mina):
1.648, enabled
Relativo Retention Timex
0.000, Disabled
1 Time Window (mine) :
0.730
Peak Selection!
.
Nearest
.
Response type: Response:
Internal Areas
Polynomial type: 1 Point of Origin:
J Pit Weighting:
I Axis Transformation':
Linear Exclude
None Nne
Units:
1
Peak-to-peak amplitude:
185
Smoothing: Window aixa (scans):
Number of smooths: Smoothing method:
Disabled
'. 1
2
Join valley threshold: Reduce peak tailing: Raise baseline maximum:
30.00 70.00 4.00
Draw vertical threshold:
Detect shoulders:
Shoulder threshold:
.
10.00 Disabled 10.00
J Relative height: . Absolute height:
Relative area: Absolute area:
50.00, Enabled 0.00, Disabled 3.00, Xoabled 0.00, Disabled
~Souo A 2 o m
BACK TO MAIN
*
LAC Olttfa_______
*S. 4
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MuChad Report
Mltfcod filBi Laic aodifildi
Compoundi
Frincidi
.Cl \KASSLYHX\FACT-l.PKOVK*thDB\022199l'BKlb
Fri Fib 26 X0t30i]9 1999
5t K613 Till Mur 09 U i 2 2 > 0 1 1999
*Compound Maati
Internal Ref. Quantify Tracii Cone, of Standardst
-
User RF Valuet User Peak factor*
Integrate Window Factor*
.
MIS [None]
TIC
Cone. A
.
'1.000000, D i n b l i d
1.000000 2.000
*Retention Time (nine)
Relative Retention Time*
Tine Window (mine) t
Peak Selection*
.
9.S91, Xnablld 0.000 Disabled 0.710
Nearest
-
Response Type* Response*
Polynomial Type* Point of Origin* Pit Weighting* Axil Traaifomationi
*I x t i n u l
Areas * .
*
t.lear Uapcmclude
None
-
-
*
Sduo 020ltf 1
units* Paak-to~peak amplitude*
185
*
*'
*Smoothing*
Window sire (scans) Number of smooths* Smoothing method*
'Join valley threshold**
Reduce peak tailing* Raise baseline maxims**
D1isabled
2
30.00 70.00 4.00
Draw vertical threshold* Dstect shoulders: Shoulder threshold*
10.00
Disabled 10.00
*
.
Relative Absolute
hheeiigghhtt**
Relative area*.
Absolute area*
, 50.00, Enabled 0.00, Disabled 3.00, Enabled 0.00, Disabled
*
L A C 0 3 lefih $ _
i *.
1 Method Report
Method file: , C:\MASSLrMX\PACT-l.PRO\MethDB\022499a.mdb
Last modified:
Fri Feb 26 10:30:39 1999
Compound:
6: ZS
Printed:
Tue Mar 09 13:22:01 1999
Ceopound taui Internal Raf.t Q uantify Traai Cone, of Standardai
Q aer XT V alu ti Uaar Faak F aeton I n te g r a ta Window F a e to n
is [None]
TIC Cone, a
.
1.000000, Diaablad
1.000000
2 .0 0 0
Retention Tina (mina) i
7.410, b a b i e d
Relative Retention Tine:
0.000, Disabled
] Tine Window (nine) i Peak Selection:
0.980 Nearest
Response Type:
external Areas - #
Polynomial Type: Point of Origin:
! Pit Weighting: Axis Transformation:
Units:
Peak-to-peak amplitude:
I Smoothing: Window sire (scans}: Number of smooths: Smoothing method:
Linear exclude None None
185
Disabled
1
2
Join valley threshold: Reduce peak tailing:' Raise baseline maximum:
30.00 70.00
4.00
Drew vertical threshold: . Detect shoulders:
Shoulder threshold:
10.00 Disabled
10.00
J Relative height: Abaolu t a haighti Relative area: Absolute area:
50.00, Enabled 0.00, Diaablad 3.00, b a b i e d
0.00. Disabled
BACK TO MAIN
--
n io mS dud r
*-
U c lo^l
Fag. i
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:& b
EM*M ADELINE 041098: FA CT E quipm ent Log 98053
Q uattro II, #6443E
PAGE
DATE: 03/01/99
ANALYST: drb
'1
.
STUDY NUMBER T O X 120 MATRIX: rat liver DESCRIPTION: liver cxtr. dilutions
FACT M ethod:
Injection vn1nmff,lQ uL
Cycle tim e: 16 m in. Electrode: Z-Spray
M obile Phase:
(A)4.0 mM N H 4CH 3C02 .
(B) MeOH_____
TN-A- or stndrd num ber (A) 99011-1-9__________
(B) TN-A-2384
Prepared (date/by): E x p ires:
` (A) 2/12/99 m ee (A) 08/12/99___________
(B) 2/20/99 drb (B) 8/18/99
Store at room tem p? [Yj o r N
. O ther._______________________
G u ard colum n: None____________ .
.
Part num ber na _______
Lot num ber na_______________________
A nalytical colu m n ; keystone betasil__________ __ ______________________________
Size: 2 x 100mm__________.
Type: c l 8 __________ Sphere size: Sum
P art num be r 105-701-2______ L ot numbe r p8f!3
Serial number 118739n
A nalytical p aram eters:
'
D rying gas (L/hr): 300
ESI nebulizing gas CL/hr) 15
LC Flow rate: 300 uL/min. B ackpressure: 120 bar
Sheath gas (L/hr)_0 .
H PL C solvent g rad ien t: long2ex
Tim e (m in.) 1) 0.00 2) 8.S 3 1.1.0
& 11.5 5) 12.5
%B 40 90
90 100 100
.
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Tuning Method Report
'
Method:
C :\MASSLYNX\RATS. PRQ\ACQUDB\PFOS
0$ #
Printed:
M o n M a r 01 17:17:40 1999
MacUliru. CHloW
fa
LMsCx/lolil
Page 1
] ] 1
MS
SOURCE ( ESP- )
Capillary Cone Extractor
J RF Lens Source Block Temp. Desolvation Temp.
I
1 Pressures
*
Analyser Vacuum
I Gas Cell
I
Set Rdbk
3.00 45 5 0.2
215500
3.00 -46 -4
215513
MSI
LM Resolution
HM Resolution
' Ion Energy
Ion Energy Ramp
MLuelnstip6lier
Rdbk
6.4e-5 5.3e-3
.*X
MS2 .
LM Resolution HM Resolution Collision Ion Energy Ion Energy Ramp Lens 8 Lens 9 Multiplier
Set Rdbk
15.0 15.0 2.0 ? 0.0 ' 55
650
Set Rdbk
15.0 15.0 20 ' 20
2.0
0 .0
40 0 650
41 18
I I
Scanning Hathed Kaport
Hathod:
C:\HMSUftOC\FATS.FJO\ACQUD8\TOX120A
Last Modified: Hon Mar 01 17:16:31 1999
Printadl
Man Mar 01 17:17:51 199
Solvant Delay | mina ) I
0.00
runotion : 1
HIM of 3 M a x Pairs ( ESP- ) '
Inter Channal Daisy ( Sacs ) t
Span ( Daltons ) t Start Tins ( Mins ) : End Tima t Mins J : Repeats t
Chinns! Parent Daughter
0.03
0.00 0.00 13.50 1 Dwell
(Sacs) Coll Energy (aVJ
1
399.00 80.00
0.25
45
2
399.00 99.00
0.25
45
3 399.00 130.00 0.25 45 -
Cone
60 60 60
Function 2 2
M M of 3 M u s Pairs ISP- )
Inter Channel Delay ( Sacs ) : Span ( Daltona ) : Start Tima ( Mins ) i End Tima ( Mins ) i Repeats l
Channel Parent Daughtar
0.03 0.00 0.00
13.50
1 Dwell
(Sica)
Coll En.rgy
(aV)
1
499.00 0.00
0.25
2
499.00 99.00
0.25
3 499.00 130.00 0.25
45 45 45
Cone
60 60 60
Function ! 3
MFM of 2 Maaa Pairs t
.
Inter Channel Delay ( Sacs ) i 0.03
Span ( Daltona ) i ~
0.00
Start Tima ( Mins ) i
* 0.00
End Tima ( Mins ) :
13.50
Repeats : .
1
Channel Parant Daughtar Dwell lS.cs) Coll Energy (.V)
1 299.00 80.00 . 0.25
2
299.00 99.00
0.25
45 45
Cone
35 35
Function : 4
HIM of 3 Maas Pairs ( ESP- J
Inter Channel Delay ( Sacs ) i Span { Daltons ) t
Start Tima ( Mins ) s. .
End Tima ( Mins ) :
Repeats t
Channel Parent Daughter
0.03
0.00
0.00 13.50
1
Dwell
(Secs) Coll Energy
(eV)
Cone ( V )
413.00 119.00 413.00 169.00
413.00 219.00
0.25 0.25 0.25
30 20 30
15 15 15
Function t 5
MEM of 3 Mass Pairs ( ESP- )
Inter Channel Delay ( Sees ) 2 Span ( Daltons j :
Start Time ( Mins ) t
'
End Time ( Mina ) 2
Kep.ats i
Channal Parent Daughtar
0.03 0.00
0.00 13.50
1 Dw.ll
(Secs) Coil Energy
(eV)
Cone ( V )
1
469.00 169.00
0:25
2 469.00 219.00 0.25
3
469.00 119.00
0.25
45 * 45 45
30 30 30
Fonation i S
M M of 3 Maas Pairs ( ESP- )
Intar Channel Delay ( Sacs ) * Span ( Daltona ) t
Start Tima ( Mins ) s End Tima ( Mina ) t
Repeats t
* Channel Parent Daughter
0.03 0.00 0.00 13.50
i Dw.ll
(Secs) Coll Energy (eV)
Cone (( V )
1
569.00 169.00
0.25
2
569.00 219.00
0.25
3 569.00 119.00 0.25
45 45 45
35 35 35
function : ?
KMC of 1 Mass Pair ( ESP- )
Zntsr Channel Delay ( Secs ) t 0.03
Span ( Daltons ) t
0.00
Start Time ( Mins ) *
0.00
End Time ( Hina ) t
13.50
Repeats I
'
,,1
Channel Parent Daughter Dwell (Sacs)
Coll Energy
(aV)
Cone ( V )1
1
427.00 80.00
0.25
4Q
60
&
u c <*/iotn
BACK TO MAIN '
Pag* 1
M asslynx - Sam ple List
Sample List: C:\MASSLYNX\rats.PRO\SampleDB\030199c.SPL Printed: Mon Mar 0117:17:14 1999
BACK TO MAIN
Page 1
I File Name File Text
.
MS File
In le t File B ottle F acto r 1 Sample
1 030199001 MeOH Blank
TOX120A
long2
91
0 Analyte
2 030199002 MeOH Blank
TOX120A
long2
91
. 0 Analyte
3 030199003 Tox 120. RBL02229-H2O B lk -1 ,2/22/99 4 030199004 Tox 120, RBL02229-RBUverBlk-1,2/22/99
TOX120A TOX120A
long2 long2
.1
2
0 Analyte 0 Analyte
5 030199005 Tox 120, RBL02229-5ppb-1.2/22/99 6 030199006 Tox 120, RBL02229-10ppb-1,2/22/99
TOX120A TOX120A
long2 long2
3 4
0 Standan . 0 Standan
] 7 030199007 Tox 120, RBL02229-25ppb-1,2/22/99 8 030199008 Tox 120, RBL02229-50ppb-1,2/22/99
TOX120A TOX120A
long2 long2
5 6.
0 Standan 0 Standan
9 030199009 Tox 120, RBL02229-1OOppb-1,2 /2 2 /9 9 1
TOX120A
long2
7
0 Standan
I 1 0 030199010 Tox 120, RBL02229-250ppb-1,2/22/99 1 1 030199011 Tox 120, RBL02229*500ppb-1,2 /2 2 /9 9 1 2 030199012 Tox 120, RBL02229-750ppb-1,2/22/99
TOX120A TOX120A . TOX120A
long2 long2 long2
8
9
10
' 0 Standan 0 Standan 0 Standan
13 030199013 Tox 120, RBL02229-1000ppb-1,2 /2 2 /9 9
TOX120A
long2 . 1 1
0 Standan
14 030199014 MeOH Blank
. TOX120A
. long2
91
0 Analyte
15 030199015 ' Tox 120 DNO. 125F-G 2,2/22/99 D5
1 16 030199016 Tox 120 DNO. 126F-G2, 2/22/99 D5 17 030199017 Tox 120 DNO. 127F-G2, 2/22/99 D5
TOX120A TOX120A TOX120A
long2 long2 long2 .
12
13 14
0 Analyte 0 Analyte 0 Analyte
18 030199018 Tox 120 DNO. 129F-G 3,2/22/99 D2
TOX120A
long2 ' 15
0 Analyte
19 030199019 Tox 120 DNO. 130F-G3, 2/22/99 D2
I 2 0 030199020 Tox 120, RBL02229-250ppb-CC1,2 /2 2 /9 9 2 1 030199021 MeOH Blank
TOX120A TOX120A TOX120A
long2 ' long2 lo n g 2.
16 17 92
0 Analyte 0 Analyte 0 Analyte
2 2 030199022 Tox 120 DNO. 131F-G 3,2/22/99 D2
TOX120A
long2
18
0 Analyte
23 030199023 Tox 120 DNO. 132F-G 3,2/22/99 D2
TOX120A
long2
19
0 Analyte
] 24 030199024 Tox 120 DNO. 133F-G3, 2/22/99 D2 25 030199025 Tox 120 DNO. 134F-G 3,2/22/99 D2
TOX120A TOX120A
long2 long2
20 21
0 Analyte 0 Analyte
26 030199026 Tox 120 DNO. 135F-G3, 2/22/99 D2
TOX120A
long2
22
0 Analyte
27 030199027 Tox 120, RBL02229-250ppb-CC2,2/22/99
TOX120A
long2
23
0 Analyte
28 030199028 MeOH Blank
'
TOX120A
long2
92
- 0 Analyte
29 030199029 Tox 120 DNO. 136F-G 3,2/22/99 D2
TOX120A
long2
24
0 Analyte
30 030199030 Tox 120 DNO. 137F-G 3,2/22/99 D2
TOX120A
long2
25
0 Analyte
31 030199031 Tox 120 DNO. 1 38F -G 3,2/22/99 D2
TOX120A
long2
26
0 Analyte
32 030199032 Tox 120 DNO. 139F-G 3.2/22/99 D2
TOX120A
long2
27
0 Analyte
i 33 030199033 Tox 120 DNO. 141F-G 3,2/22/99 D2 34 030199034 Tox 120, RBL02229-250ppb-CC3,2/22/99
TOX120A TOX120A '
long2 long2
28 29
0 Analyte 0 QC
35 030199035 MeOH Blank
TOX120A
long2
93*
0 Analyte
J 38 030199036 Tox 120 DNO. 142F-G 3,2/22/99 D2 37 030199037 Tox 120 DNO. 143F-G 3,2/22/99 D2 38 030199038 Tox 120, RBL02229-250ppb-CC4, 2/22/99
39 030199039- MeOH Blank
TOX120A
TOX120A TOX120A TOX120A
long2
long2 k>ng2 long2
30 ; 31 32 93
0 Analyte
0 Analyte 0 QC O Analyte
40 030199040 Tox 1 2 0 ,106F-G1-250ppb-MS, 2/22/99
TOX120A
long2
33
0 Analyte
41 030199041 Tox 1 2 0 ,1 06F-G1-250ppb-MSD, 2/22/99
TOX120A
long2
34
0 Analyte
1 42 030199042 Tox 120, RBL02229-250ppb-CC5,2/22/99
TOX120A
long2
35
0 QC
43 030199043 MeOH Blank
TO X120A .-V
long2
93
. 0 Analyte
44 030199044 Tox 120, RBL02229-H2O B lk -2 ,2/22/99
J 45 030199045 .Tox 120, RBL02229-RBLiverBlk-1,2/22/99 46 030199046 Tox 120, RBL02229-5ppb-2,2/22/99' 47 030199047 Tox 120, RBL02229-1Oppb-2,2/22/99 48 030199048 Tox 120, RBL02229-25ppb-2,2/22/99
TOX120A TOX120A TOX120A TOX120A TOX120A
long2 long2 long2 long2 long2
. '
36 37 38 39 40
0 Analyte 0 Analyte 0 Standai 0 Standai 0 Standai
49 030199049 Tox 120, RBL02229-50ppb-2,2/22/99
J 50 030199050 Tox 120, RBL02229-100ppb-2,2/22/99 51 030199051 Tox 120, RBL02229-250ppb-2,2/22/99
TOX120A TOX120A TOX120A
long2 long2 long2
41 . 42 43
0 Standai 0 Standai 0 Standa
52 030199052 Tox 120, RBL02229-500ppb-2,2/22/99 53 030199053 Tox 120, RBL02229-750ppb-2,2/22/99
TOX120A TOX120A
long2 long2
44 45
0 Standa 0 Standa
I 54 030199054 Tox 120, RBL02229-1000ppb-2,2/22/99
TOX120A
long2
46
0 Standa
j V\ iVVaklW 03/*/
OV/oy
1
FACT: DATA REVIEW SUMMARY FORM
BACK TO MAIN
Date: 0 3 / o s M
Analyst:
FACT Study Number ~Tb'C 1 ^ 0
Matrix Time Points
Xixsf&' 13
Dilutions?
J Analyte 1:
V D S>1V
Acceptable lin ear Range:
Q fb
LOQ for this data s e t
Re-evaluated fa: different range?
A^o
Analyte 4:
Acceptable Linear Range: / '^ 1 QDO
LOQ for this data set: / Q
______
Re-evaluated for different range? f\ ) O
Anafyte5:
Acceptable linear Range: /
OQ3ppk>
LOQ fix this data set: 7 ^ 0
Re-evaluated fix different range? $>
Teio*w cLk'
V
Analyte 2:
B3S
Acceptable Linear Range: / Q ** )j
LOQ far this data se t
J
_______
Re-evaluated fix different range?
fJ o
J Analyte 3:
Acceptable Linear Range:
&c&ppb
3 LOQ fix this data set: Re-evaluated for different range?
t
Analyte 6: ~
S ie * /
Acceptable
LOQ for this data set
Re-evaluated for different range? a / d
A nalyte 7:
Acceptable Linear Range: LOQ for this data set _____ Re-evaluated for different range?
BACK TO MAIN
Quantify Ccapound Suanary Kaipert TOX 120 DHO Rat Liras Group. i t ]
Saapl. List: M:VRAI3.PBO\3uplMa\030199a
Last nodifiad: Mon Mas 08 08:49:2 1*99
Mathod:
M:\rats.HW\MathEB\010199a
Last nodifiad: Mon Mas 08 10:03:92 1999
Job Coda:
P rin ta d :
Mon Mas 08 10:35:49 1999
w >.
cjltttf
Paga 1
Cooperad 1 : FFOS
Kano 1 030199001
S o c ia lis t Taxt MaOK Blank
'
* l t d Cono
RI
A nalyt
7.929
Araia D H .T act. Cono fla g %Dv
12509 1
286.71 MK
2 030199002 HsOH Blank
A n aly t
7.913
1971 1
4.03 MK
3 030199003 Toa 120, RBL02229-H2O B lk-1, 2/22/9
A n aly t
7.959
1609 1
0.00 MK
4 030199004 Tox 120, BBL0222-RBUvarBlk-l, 2/22/99
A n aly t
7.964
22(5 1
13.96 MK
5 030199005 Tox 120, BBL02229-Sppb-1, 2/22/99
Standa
5.99 8.073
3267 1 '
41.14 bbX 587
6 030199006 Tox 120, ABL02229-10ppb-l, 2/22/99
Stands
12.00 7.899
2555 1
22.20 fab
85
7 030199007 Tox 120, RBL0 2229-25ppb-1 , 2 /22/99
Standa
29.90 7.927
2937 1
32.36 bb
8
8 030199008 Tox 120, RBL02229-50ppb-l, 2/22/9
Standa
59.90 8007
(107 1
. 63.44 Hf
6
9 030199009 Tox 120, BBL02229-100ppb-l, 2/22/9
Standa 120.00 8.023
6367 1
123.50 MK
3
10 030199010 Tox 120, BBL02229-250ppb-l, 2/22/99
S tanda 299.00 8.002
12968 1
299.44 MK
0
3 11 030199011 12 030199012 13 030199013
Tox 120, RBL02229-50Oppb-1, 2/22/99 Tox 120, RBL02229-75Oppb-1, 2/22/99 Tox 120, RBL02229-1000ppb-l, 2/22/9
Standa Standa Standa
599.00 898.00
1198.00
8.043 8.017 -8.000
2SS(( 1 37384 1 (5(78 1
633.05 bb 947.68 bb 1162.75 bb
6 6 -3
14 030199014 MaOH B lank
A n aly t
7.871
1341 1
0.00 bb
15 030199015 Tax 120 DHO. 125T-G2, 2/22/99 DS
A n aly t
7.858
1796 1.
2.05 bb
18 030199016 Tox 120 DHO. 126F-G2, 2/22/9 DS
. Bnalyt
7.875
1820 1
2.69 bb
17 030199017 Tox 120 DHO. 1277-C2, 2/22/9 DS
A n aly t
7.857
16(5 1
0.00 bb
18 030199018 Tox 120 DHO. 1297-G3, 2/22/99 D2
A nalyt
7.895
228 1
15.14 bb
19 030199019 Tex 220 DHO. 1307-4B, 2/22/99 D2
A nalyt
7 879
2367 1
17.21 MK
20 030199020 Tox 120, RBL02229-250ppb-CCl, 2/22/99
QC
299.00 8.051
11524 1
,260.53 bb
-13
21 030199021 MaOK Blank
A nalyt
7.896
1(S 1
0.00 bb
22 030199022 Tox 120 DHO. 1317-03, 2/22/99 D2
A n aly t
7.819
2(98 1
20.70 MK
T 23 030199023 24 030199024 25 030199025
Tox 120 DHO. 1327-03, 2/22/99 D2 Tox 120 DHO. 1337-63, 2/22/99 D2 Tox 120 DHO. 13*7-63, 2/22/9 D2
A n aly t
A n aly t A nalyt
7.827 7.901* 7.822
2(70 1 2356 1 2315 1
19.95 MK 16.92 MK 15.83 MK
26 030199026 .T o x 120 DHO. 1357-63 , 2/22/99 D2
A n aly t
7.833
2363 1
17.12 MK
27 030199027 Tox 120, HBL02229-250ppb-CC2, 2/22/99
oc
299.00 8.005
10(71 1
232.56 MK -22
28 030199026 MaOK B lank
A n aly t
7.919 ' 14(3 1
0.00 bb
29 030199029 Tox 120 DHO.. 1367-63, 2/22/99 D2
A n aly t
7.887
2196 1 .
12.67 MK
30 030199030 Tox 120 DHO. 1377-63, 2/22/99 D2
A nalyt
7.869
1888 1
4.49 MK
31 030199031 Tox 120 DHO. 1387-63, 2/22/99 D2
A nalyt
7.854
1834 1
3.07 KM
32 030199032 Tox 120 DHO. 1397-63 , 2/22/99 D2
A n aly t
8.020
2358 1
16.99 bb
33 030199033 Tox 120 DHO. 1417-63, 2/22/99 D2
A nalyt
7.869
2612 1
23.74 bb
34 030199034 Tox 120, RBL02229-250ppb-CC3, 2/22/99
QC
299.00 8.017
11(01 1
257.26 bb
-14
35 030199035 MaOH Blank
A nalyt
7.933
1302 1
0.00 bb
36 030199036 Tox 120 DHO. 1(27-63, 2/22/99 D2
A n aly t
7.854
2228 1
13.53 MK
37 030199037 Tox'120 DHO. 1(37-63, 2/22/99 D2
A nalyt
7.929
2526 1
21.44 bb
38 030199038 Tox 120, RBL02229-250ppb-CC(, 2/22/99
. QC
299.00 8.023
11556 1
261.39 bb -13
39 030199039 Mam B lank
A n aly t
7.932
1265 1
0.00 MK
40 030199040 Tox 120, 1067-Gl-250ppb-KS, 2/22/99
A n aly t
8.034
2(68 1
610.36 KM
1 41 030199041 42 030199042
Tox 120, 1067-61-250ppb-MSD, 2/22/9 Tox 120, BBL02229-2S0ppb-OCS, 2/22/9
43 030199043 MaOH B lank
A n aly t
QC A n aly t
299.00
8.015 8.043 7.864
25982 1 11767 1
1(0 1
644.70 MK 266.98 bb
0.00 KM
-11
44 030199044 Tox 120, BBL02229-H20 B lk-2, 2/22/9
'
A nalyt
7.885
168 1
0.00 bb
45 030199045 Tox 120, BBL02229r BBLivarBlk-l, 2/22/99
A n aly t
7.898
13(1 1
0.00 MK .
i 46 030199046 47 030199047 48 030199048
Tox 120, BBL02229-5ppb-2, 2/22/9 Tox 120, RBL02229-10ppb-2, 2/22/99 Tox 120, HBL02229--25ppb-2, 2/22/99
Standa Standa .Standa
5.99 7.915
12.00' 7.845 29.90 7.995
1884 1
1952 1 2830 1
1.39 bb
6.18 MK 29.51 MM
-27 -49
-1
49 030199049 Tox 120, RBL02229-50ppb-2, 2/22/9
Standa
59.90 8.011
3802 1
25.34 bb
-a
SO 030199056 51 030199051
Tax 120, BBL02229-100ppb-2, 2/22/9 Tox 120, BBL02229-2S0ppb-2, 2/22/9
Standa Standa
120.00 7.988 299.00 8.002
6276 1 1277 1
121.15 bb 293.88 bb
1 -2
52 030199052 Tox 120, 9BL02229-S00ppb-2, 2/22/99
Standa 599.00 7.997
23131 1
568.95 MM
-5
S3 030199053 Tox 120, 8BL02229--750ppb-2, 2/22/9
Standa 898.00 8.045
32672 1
822.46 bb
-8
54 030199054 Tox 120, BBL0222-1000ppb-2, 2/22/99
Standa 1198.00 8.031
..(x8811 1
1251.30 bb
4
J
*adollne041098:: quantitated and printad 03/08/99 by deb
I
nad*llna041098:: quantitated and prlntad 03/08/99 by deb
8 8 8 ? ? ? 8 8 ? f P 8 ^ifi S i ^ ^ g 88 g p f 8 8 8 f i P
^ 8 8 s f ^8 s s f Sfili!
SSSSSBSSSSS
i fl!S g 15i s ar i l i H | P P r t P P P i P P p p11| *i1P1V P1 vri 8ii; ; ; ; ; ; ; ; ; ' 8 a a !
;$s I ss1 m B S b S S S b s b s b S b b b b b S ^ s s s k SSSSSSST,
!S5 -- Si $ 58 2 S
5 S ' S S S S """"' ;
IFI
1 IVi lSi{
I il
a n a l J S 383 35
K h I o ? la
S Bg " 8 0 M/8'H?B w" "
1 *f*f
1nMe O'NW MH (CM H
iaoN(
K> M
ARAftAAAAAA
U^ tAUAUAKUI* AA AUUU<HAOA
AAA
touO(s*--enJ -iJn
H A O
liiillsHss 111 I
00 O U M
isSsSKoo SSo 5 . s s s s s s s s i s oui: s
3 5 3 3 3 3 3 3 ??* ? ? 3 ?
H
oii.K.iC.LSS
I
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I
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3 3?
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rm*
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AAAAAAAAAA A AAAAAAAAA
isissss
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,3S S S S 5 K S 8
sssssbssss
33333333g?
CD
> O 7
?
BACK TO MAIN
Quantify Compound S m e a r y Raport TOX 120 W O Rat U m Groups 2 ft 3
Saapla List: M:\RAM.PR0\SasplsBH\030199o
Last aodifisd: Mon Mar 08 09:49:28 1999
Mathod:
M:\rats.PRO\MathU8\030199a
Last aodifiad: Mon Mar 08 10:03:52 1999
Job Coda:
Printed:
Mon Mar 01 10:35:4 IS
Compound 3:
Mans
1 030199001 2 030199002 3 030199003 4 030199004 5 030199005 6 030199006 7 030199007 8 030199008 9 030199009 10 030199010
11 030199011 12 030199012 13 030199013 14 030199014 IS 030199015 18 030199016 17 030199017 18 030199018 19 030199019 20 030199020 21 030199021 22 030199022 23 030199023
24 030199024 25 030199025 26 030199026 27 030199027 28 030199028 29 030199029 30 030199030 31 030199031 32 030199032 33 030199033 34 030199034 35 030199035 36 030199036 37 030199037 38 030199038 39 030199039 40 030199040 41 030199041 42 030199042 43 030199043 44 030199044 45 030199045 46 030199046 47 030199047 48 030199048 49 030199049 50 030199050 51 030199051 52 030199052 53 030199053 54 030199054
aaplaliat last
,
MaOH Blank
MaOH Blank
Ion 120, BBL02229-H2O Blk-1, 2/22/99
Ton 120, KBL02229-KBX*ivarBlk-l, 2/22/99
Ton 120, *BL0222-5ppb-l, 2/22/99
Ton 120, RBL02229-10ppb-l, 2/22/99
Ton 120, KBL02229-25ppb-l, 2/22/99
Ton 120, RBL02229-50ppb-l, 2/22/99
Ton 120, SBL02229-100ppb-l, 2/22/99
Ton 120, RBLO2229-250ppb-l, 2/22/99
Ton 120, BBL02229-500ppb-l, 2/22/99
Ton 120, 8BL02229-750ppb-l, 2/22/99
.Ton 120, NBL02229-1000ppb-l, 2/22/99
MaOH Blank
'
Ton 120 DMO. 125F-432, 2/22/99 DS
Ton 120 DMO. 126F-62, 2/22/99 D5
Ton 120 DMO. 127F-G2, 2/22/99 D5
Ton 120 DMO. 129F-G3, 2/22/99 D2
Ton 120 DMO. 130F-G3, 2/22/99 D2
Ton 120, KBL02229-250ppb-CCl, 2/22/99
MaOH Blank
Ton 120 DMO. 131F-G3, 2/22/99 D2
Ton 120 DUO. 1321-03, 2/22/99 D2
Ton 120 DNO. 133F-G3, 2/22/99 D2
Ton 120 DMO. 134P-G3, 2/22/99 D2
.Ton 120 DNO. 13SF-03, 2/22/99 D2
Ton 120, MBL02229-250ppl>-CC2, 2/22/99
MaOH Blank
-
Ton 120 DNO. 136F-G3, 2/22/99 D2
Ton 120 DNO. 137F-G3, 2/22/99 D2
Ton 120 DNO. 138F-G3, 2/22/99 D2
Ton 120 DNO. 139F-G3, 2/22/99 D2
Ton 120 DNO. 141F-G3, 2/22/99 D2
Ton 120, RBL02229-250ppb-CC3, 2/22/99
MaOH Blank
Ton 120 DNO. 142F-G1, 2/22/99 D2
Ton 20 DNO. 143F-G3, 2/22/99 D2
.
Ton 120, BBL02229-2S0ppb-CC4, 2/22/99
MaOH Blank
Ton 120, 106F-Gl-250ppb-MS, 2/22/99
Ton 120, 106F-61-250ppb-MSD, 2/22/99
Ton 120, BBL02229-25Oppb-CCS, 2/22/99
MaOH Blank
Ton 120, BBL02229-H2O Blk-2, 2/22/99
Ton 120, RBL02229-NBUvarBlk-l, 2/22/99
Ton 120, BBL02229-5ppb-2, 2/22/99
Ton 120, RBL02229-10ppb-2, 2/22/99 Ton 120, KBL02229-25ppb-2, 2/22/9
Ton 120, BBU>2229-50ppb-2, 2/22/99
Ton 120, RBL02229-100ppb-2, 2/22/99
Ton 120, *BW2229-2S0ppb-2, 2/22/99
Tan 120, *BL02229-500ppb-2, 2/22/99
Ton 120, BBL02229-750ppb-2, 2/22/99
Ton 120, BBL02229-1000ppb-2, 2/22/99
'
aft
n lctifft
Papa 3
Std Cono
Analyt
Analyt
Analyt
Analyt
Standa
5.99
Stands
12.00
Stands
29.90
Stands
59.90
Stands 120.00
Stands 299.00
Stands' 599.00
Stands 898.00
Stands 1198.00
Analyt
Analyt
Analyt
Analyt
Analyt
Analyt
QC 299.00
Analyt
Analyt
Analyt
Analyt
Analyt
Analyt
QC 299.00
Analyt
Analyt
Analyt
Analyt
Analyt
Analyt
QC 299.00
Analyt
Analyt
Analyt
QC 299.00
Analyt
Analyt
Analyt
QC 299.00
Analyt
Analyt
Analyt
Stands
5.99
Stands Stand*
12.00 29.90
Stands
59.90
Stands 120.00
Stands 299.00
Stands 599.00
Stands 898.00
Stands 1198.00
KT
Ai m Dll.Fast.
Cano Flap. %DV
4.128 4.138 4.124 4.143 4.213 4.20
>7 1 212 1 399 1 659 1
1110 1 1381 1
48.06 bb 153.71 bb 323.86 bb 561.92 bb 974.76 bb 1222.81 bb
-20 28 8 -6 9 2
4.147
401 1
4.171
473 1
325.85 bb
9
392.1 bb
31
4.03
4.046 4.390 4.297 4.057
389 1
389 1 377 1 258 1 427 1
4.247 4.247 4.15 4.163 4.145 4.129
. 102 1 176 1 388 1 601 1
1036 1 3,313 1
314.75 bb
5
314.84 bb
303.93 bb 195.04 bb 350.10 bb
h
5 17
52.25 bb 120.05 bb 314.63 bb 508.68 bb 906.92 bb 1160.16 bb
-13 0 5
-15 1
--3
a*deline04108:: quantitated ad printed 03/08/9 by drb
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11.91 MH 26.17 MM 61.26 K M 106.51 M M 311.01 MK 591.1 bb 965.76 bb 1192.13 bb 77.90 bb 77.88 MK .287.96 bb 106.08 bb 99.25 bb 81.58 M K 72.71 bb 9.69 M M 250.93 MH
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6.805 6.883 7.310 6.981* 6.823 6.895 7.326 6.916 7.316
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041098:: quantitatad and printad 03/08/99 by drb
i f i
6 1 275 1 372 1 1 1 727 1 311 1 301 1 266 1 329 1 <18 1 173 1 281 1 15 1 260 1 352 1 500 1 163 1 257 1 218 1 222 1 226 1 196 1 190 1 119 1 209 1 393 1 535 1 703 1
McUlin*041098: : quantitated and printed 03/08/99 by drb
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BACK TO MAIN
Quantify Ccapound Samaxy Raport TOX 120 DUO Rat U v i t Group U S
Saapl* List: M:\RATS.PBj0\SMvlaDB\030199o
Last nodifiod: Mon Mar 08 09:49:28 1999
Mathod:
M:\rats. PRO\MathDB\030199o
Last nodifiod: Mon Mar 08 10:03:92 1999
Job Coda:
Printad:
Mon Iter 08 10:35:49 1999
Compound 8: ?alaar969
Sana
laaplaliat Tart
030199001 MaOK Blank
030199002 MaOH Blank
3 030199003 Tor 120, U U 2 2 2 9 - H 2 0 Blk-1, 2/22/99
030199004 Tox 120, BBL0222-8BLivarBlk-l, 2/22/99
S 030199005 Tor 120, KBM222-5ppb-l, 2/22/99
i 030199006 Tor 120, *BL02229-10ppb-l, 2/22/99
7 030199007 Tox 120, 2BL02229-25ppb-l, 2/22/99
0 030199000 Tox 120, RBL02229-50ppb-l, 2/22/99
9 030199009 Tox 120, *BL0222-100ppb-l, 2/2?/
10 030199010 Tox 120, RBL02229-250ppb-l, 2/22/99
11 030199011 Tox 120, RBL02229-500ppb-l, 2/22/99
12 030199012 Tox 120, BBL02229-750ppb-l, 2/22/99
13 030199013 Tox 120, *BL02229-1000ppb-i, -2/22/99
II 03019901 MaOH Blank
15 030199015 Tax 120 m O . 125F-G2, 2/22/99 05
16 030199016 Tox 120 OHO. 126F-62, 2/22/99 D5
17 030199017 Tox 120 DUO. 1277-G2 2/22/99 D5
18 030199018 Tox 120 m o . 129F-63, 2/22/99 02
19 030199019 Tox 120 rno. 1307-63, 2/22/99 02
20 030199020 Tox 120, RBL02229-250ppb-CCl, 2/22/99
21 030199021 MaOH Blank
22 030199022 Tox 120 OHO. 1317-63, 2/22/99 02
23 030199023 Tox 120 OHO. 1327-63, 2/22/99 D2
21 030199024 Tox 120 OHO. 1337-63, 2/22/99 D2
25 030199025 Tox 120 OKO. 1347-63, 2/22/99 D2
26 030199026 . Tox 120 m o . 135F-63, 2/22/99 D2
27 030199027 Tox 120, RBL02229-250ppb-CC2, 2/22/99
28 030199028 HOOK Blank
29 030199029 Tox 120 m o . 1367-63, 2/22/99 D2
30 030199030 Tox 120 m o . 1377-63, 2/22/99 02
31 030199031 Tox 120 OHO. 1307-63, 2/22/99 02
32 030199032 Tox 120 OHO. 1397-63, 2/22/99 D2
33 030199033 Tox 120 DNO. 1417-63, 2/22/99 02
34 030199034 Tox 120, RBL02229-250ppb-CC3, 2/22/99
35 030199035 MaOH Blank
36 030199036 Tax 120 m o . 1427-63, 2/22/99 02
37 030199037 . Tok 120 OHO. 1437-63, 2/22/99 02
38 030199038 Tox 120, HBLQ2229-250ppb-CC4, 2/22/99
39 030199039 MaOH Blank
40 030199040 Tox 120, 106F-61-250ppb-MS, 2/22/99
41 030199041 Tox 120, 106F-61-250ppb-HSD, 2/22/99
42 030199042 Tox 120, HB02229-250ppb-CCS, 2/22/99
43 030199043 MaOH Blank
44 030199044 Tox 120, RBL02229-H2O Blk-2, 2/22/99
45 030199045 Tox 120, BBX02229-BBLivarBlk-l, 2/22/99
46 030199046 Tox 120, BBL02229-5ppb-2, 2/22/99
47 030199047 48 030199048
Tox 120, RBL02229-10ppb-2, 2/22/99 Tox 120, RB1.02229-25ppb-2, 2/22/99
49 030199049 Tox 120, *BBL02229-50ppb-2, 2/22/99
SO 030199050 Tox 120, RBL02229-100ppb-2, 2/22/99
51 030199051 Tox 120, HBL02229-250ppb-2, 2/22/99
52 030199052 Tax 120, BBL02229-500ppb-2, 2/22/99
53 030199053 Tax 120, RBL02229-750ppb-2, 2/22/99
54 030199054 Tox 120, BBL02229-1000ppb-2, 2/22/99
.
Paga 8
Typa Analyt Analyt Analyt Analyt Stand Stand Stand Stand Stand Stand Stand Stand Stand Analyt Analyt Analyt Analyt Analyt Analyt QC Analyt Analyt Analyt Analyt Analyt Analyt QC Analyt Analyt Analyt Analyt Analyt Analyt QC Analyt Analyt Analyt QC Analyt Analyt Analyt QC Analyt Analyt Analyt Standa Standa Stand Stand Stand Standa Standa Standa Standa
ltd Cono
5.99 12.00 29.90 59.90 120.00 299.00 599.00 090.00 1190.00
9.414 9.429 9.414 9.460 9.424 9.401
299.00 9.469
9.419
299.00 9.422
299.00
9.428 9.444 9.427
9.420 299.00 9.429
299.00
9.443 9.427 9.450
5.99 12.00 29.90 59.90 120.00 299.00 599.00 898.00
1198.00
9.407 9.431
9.413 9.426
9.414 9.454 9.440
As m Dil.raot. Cano Tlags %Dv
117 1 238 1 528 1 896 1 1478 1 1532 1
56.03 bb 111.01 bb 230.62 bbX 399.67 bbX 654.20 bbX 677.92 bbX
-2 -7 -20 -33 -27 -43
354 1 105 1 507 1
. 162.7 bb
-46
53.86 bb
229.52 M M
-23
56 1
32.09 M M
4 1
29.38 MM
481 1 . 218.05 bb
-27
48 1 544 1
446 1 357 1 337 1
28.51 MM 245.83 bb
203.94 bb 163.92 M M 155.01 bb
-18 -48
`5. .
64 1 .132 1
242 1 676 1 ($6 1 1154 1 172 1
36.56 MM 66.50 bb
113.63 bb 393.37 bb 399/62 bbX
512.64 bbX 764.09 bbX
19 9
-5 1
-33 -43 -36
aadUna041098:: quantitatad and printad 03/08/99 by drb
BACK TO MAIN
Quantify C o ^ a u n d S u u a r y apart TOX 120 DUO Kat Livor Croups 2 fi 3
spia List: M:\RAXS.PBO\SssplsCB\030U9a
L u t asdiXiad: Man Iter 08 09:49:26 1999
Mathod:
M:\rats.9KO\MstbDB\030199a
L u t wxllfi.d: Man M u 08 10:03:52 1999
Jab Coda:
Printad:
Man M U 08 10:35:49 1999
Caspound 7: surr
Ki m 1 030199001 2 030199002 3 030199003 4 030199004 S 030199005 6 030199006 7 030199007 8 030199008 9 030199009 10 030199010
11 030199011 12 030199012 13 030199013 II 030199014 15 030199015 16 030199016 17 030199017 18 030199018 19 030199019 20 030199020 21 030199021 22 030199022 23 030199023 21 03019924 25 030199025 25 030199026 27 030199027 28 030199028 29 030199029 30 030199030 31 030199031 32 030199032 33 030199033 31 030199031 35 030199035 36 030199036 37 030199037 38 030199038 39 030199039 10 030199010 11 030199041 12 030199012 13 030199013 11 030199011 15 030199015 16 030199016 17 030199047 <0 030199048 19 030199049
50 030199050 51 030199051 52 030199052 53 030199053 51 030199051
Sanplolist Tort
MaOH Blank
MaOH Blank
Tox 120, KBL02229-H2O Blk-1, 2/22/99
Tox 120, BBL02229-BBLivarBlk-l, 2/22/99
Tox 120, RBL02229-5ppb-l, 2/22/99
Tox 120, RBL02229-10ppb-l, 2/22/99
Tox 120, 8BL02229-2$ppb-l, 2/22/99
Tox 120, BBL02229-50ppb-l, 2/22/99
Tox 120, BBL02229-100ppb-l, 2/22/99
Tox 120, BBL02229-250ppb-l, 2/22/99
Tox 120, RBL02229-500ppb-l, 2/22/99
Tox 120, BBL02229-750ppb-l, 2/22/99
Tox 120, BBL02229-1000ppb-l, 2/22/99 '
MaOH Blank
Toa 120 ONO. 125P-C2. 2/22/99 BS .
Tax 120 IMO. 126P-62, 2/22/99 DS
Tox 120 IMO. 1271-62, 2/22/99 SS
Tox 120 ONO. 1291-63, 2/22/99 D2 Tox 120 OHO. 1301-63, 2/22/99 02
Tox 120, RSL02229-2S0ppb-CCl, 2/22/99 MaOK Blank
Tox 120 ONO. 131P-63, 2/22/99 D2
Tox 120 ONO. 132P-63, 2/22/99 D2
Tox 120 ONO. 1332-63, 2/22/99 D2
Tox 120 ONO. 1342-63, 2/22/99 02.
.Tox 120 ONO. 1352-63, 2/22/99 02
Tox 120, K8L02Z29-250ppb-CC2, 2/22/99
KaOH Blank
'
Tox 120 ONO. .1362-63, 2/22/99 D2
Tox 120 ONO. 1372-63, 2/22/99 D2
Tox 120 OHO. 1382-63, 2/22/99 02
Tox 120 ONO. 1392-63, 2/22/99 02
Tox 120 ONO. 1412-63, 2/22/99 02
Tox 120, BBL02229-250ppb-CC3, 2/22/99 .
MaOH Blank
Tox 120 ONO. 1122-63, 2/22/99 02
Tox 120 ONO. 1132-63, 2/22/99 02
Tox 120, BBL02229-250ppb-CC4, 2/22/99
MaOH Blank
Tox 120, 1062-61-2SOppb-M5, 2/22/99
Tox 120, 1062-61-250ppb-MSD, 2/22/99
Tox 120, RBL02229-250ppb-CCS, 2/22/99
MaOH Blank
Tox 120. RBL02229-H2O Blk-2, 2/22/99
Tox 120, SBL02229-*BLivarBlk-l, 2/22/99
Tox 120, BBL02229-5ppb-2, 2/22/99
Tox 120, BBL02229-10ppb-2, 2/22/99 Tox 120, BBL02229-25ppb-2, 2/22/99 TOX 120, KBL02229-50ppb-2, 2/22/99
Tox 120. RBL02229-100])pl>-2, 2/22/99 Tox 120, BBL02229-2S0ppb-2, 2/22/99
Tox 120, BBL02229-500ppb-2, 2/22/99
Tox 120, RBL02229-?50ppb-2, 2/22/99
Tox 120, 8BL02229-1000ppb-2, 2/22/99
Pago 7
Typ
Analyt Analyt Analyt Analyt Stand Stand Stand Stand Stand Stand Stand Stand Stand Anailyt Analyt Analyt Analyt Analyt Analyt QC Analyt Analyt Analyt Analyt Analyt Analyt QC Analyt Analyt Analyt Analyt Analyt Analyt QC Analyt Analyt Analyt QC Analyt Analyt Analyt QC Analyt Analyt Analyt Stand Stand Stand Stand Stand Stand Stand Stand Stand
Std Cono
1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00
1.00
1.00
1.00
1.00
1.00
1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00
RT
7.334 7.344 7.275 7.277 7.302 7.303 7.315 7.299 7.249 7.308 3.297
7.258
7.293
7.215
7.275 7.284 7.302 7.286 7-300 7.281 7.295 7.287 7.252 7.234
At m DU.Saot.
Cane Flag %Dv
494 1 579 1 1017 1 1248 1 1111 1 1149 1 1240 1 1233 1 1192 1 1245 1 1117 1
0.11 M 0.18 MM 0.85 MM 1.04 MM 0.92 MM 0.95 MM 1.03 MM 1.02 m 0.99 M K 1.03 MM 0.93 M M
-59 -52 -15
4 -8 -5
3 2 -1 3 -7
908 1
0.75 M M
-25
1063 1 191 1
0.88 M M
-12
0.11 M M
-59
1010 1
1172 1 1377 1 1293 1 1141 1 1108 1
1311 1 1198 1
958 1
.1229 1 .'V
0.81 MM 0.97 Hk 1.11 HM 1.07 HK 0.95 MM 1.17 HM
1.11 MH 1.00 HM 0.80 HM 1.02 MM
.
-16 -3 11 7 -s' 17
11 0
-26 2
adal1na041098: : quantitatad and printad 03/06/99 by drb
BACK TO MAIN
1 _ _ _ _ _ _ _ _ _ :_ _ _ _ _ _ _ 1 _ _ _ _ _ _ _ _ _ _ _ n -*
Quantify Calibration Itapoit
5S* 1
, TOX 120 EMO Hat Livax Occupa 2 0 3
.
I Calibration: K:\rata.9M0\CumflB\030199a
I La*t nodlfiad: Men Mac 08 10:10:07 198
Printed:
Mon Mar 01 10:36:09 199
''
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aadellneO41098;: quantitat*d and printed 03/08/99 by deb
Quantify Calibration taport
IOX 120 DM0 tut tirar Croup 2 C 3
Calibration: M:\rate.M0\CurvaCB\030199o
l u t nodifiad: Mon Mar 08 11:06:52 1999
Printed:
Mon Mar OS 11:10:07 I M O
.
Com pound 2 name: PFH S
.
C orrelation co efficien t r = 0.999248, r* 2 = 0.998498
C alibration curve: 20.5223 * x + 973922
R esponse type: E xternal Std, A rea
C urve type: L inear, O rigin: E x d u d e, W eighting: N ull, A xis trans: N one
3
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I udallzw041098: : quantittad and prinfcad 03/08/99 by drb
BACK TO MAIN
Paga 2 '
'
1
Quantify Calibration ftaport TCX 120 DHO Rat Livar Group* 2 fi 3
1 Calibration: M:\rats .PRO\CurrCB\030199 Last nodifiad: Mo n Mar 08 10:10:07 1999
Printed:
Mon Mas 08 10:36:05 1999
1
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* .
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Quantify Calibration Report TQX 120 DHO Bat Liver Groups 2 3
Calibration: M: \rata .SRO\CurvaDB\030199o
1 Last modified; Mon Mar 08 10:10:07 1999
printed:
Mon Mar 08 10:38:05 1999
1 Com pound 4 name: POAA C orrelation co efficien t t = 0.998817, r* 2 = 0.997636
'
C alibration curve: 5*20938 * x + 18.1144
lesp o n se type: E xternal Six], A rea
C urve type: Linear, O rigin: Exclude, W eighting: N u ll, A xis tran s: N one
J
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I aadallna041098: : quantitated and printed 03/0*/ by dtb
>
BACK TO MAIN
Quantify Calibration fapect TOX 120 ta(0 Rat Hirer Groins ] ( )
Calibration: M:\rats.PR0\CttrvaDB\0J0199o
last Modified: Mon Mar 08 10:10:07 1098
Printed:
Mon M u 08 10:38:05 1989
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diline04i098;t quantitated and printed 03/08/99 by dtb
BACK TO MAIN
Paga 5 .
'
1
Quantify Callbration Baport TQX 120 DKO Kat l i m Sroups I I I
] Calibratimi M:\rats.M0\CusvaEB\O3O199a last aodifiad: Mon Mas 00 10:10:07 190
Psintad:
M m Mas 09 10:39:05 199
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9*9 * .
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Quantify Calibration JLaport TOX 120 DUO Rat Livar Group* 2 1 ]
Caliteation: M:\sats.8MO\CurraDB\010199a
l u t aodifiod: Mon Mar 08 10:10:07 1899
Pxintod:
Meo Mas 08 10:38:05 1999
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Method Report
Method file: Last nodi fled:
Compound:
Printed:
K:\rats.FItO\HethDB\030199c.sdb Mon Mar 08 10:03:52 19990 1: FFOS Mon Mar 08 10:40:02 1999
Compound Heme: Internal Ref.: Quantify Trace: Cone, of Standards:
User UP Valu:
.
User Peak Factor:
Integrate Window Factor:
Peak Location
FFOS [None] TIC Cone. A
1.000000, Disabled 1.000000 2.000
Retention Time (mine): Relative Retention Time: Time Window (mine): *
0.130, Inablad 0.000, Disabled 0.793
Fak Selection:
Response
Response Type: Response:
eareat .
.
External Areas
Calibration Curves
Polynomial Type: Point of Origin: Pit Weighting: Axis Transformation:
Linear Exclude None None
.
Concentration
Units: Noise
*
Feek-to-peak aaplitude: Smooth
' 150
Smoothing: Window size (scans): Number of smooths: ' Smoothing method:
Baselines
Disabled 3 2
Join vallay threshold: Reduce pak tailing: Raisa baseline aaximin::
Peak Separation
Draw vertical threshold: Detect shoulders:
Shoulder threshold:
*
20.00 100.00 0.00
10.00 Disabled 10.00
' `
Reasons Thrashold
Relative height: Absolute height: Relative eree: Absolute area:
.
1.50, Disabled 0.00, Disabled 2.00/ Disabled 0.00, Inablad
'
'
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M r i d L ___ o ^ m .. LAc m l n s k l 1
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Method Report
Method fil*: Last modified:
Compound:
Printed:
M:\rats.PRO\MethDB\030199e.mdb Mon Mar 08 10:03:52 19990
2: PPHS
Hon Mar 08 10:10:02 1999
Compound N o w : Internal Ref.: Quantify Trace: Cone, of Standards:
PFH3 {None] TIC Cone. A
'
User W Value: User Peak Factor: Integrate Window Factor:
1.000000, Disabled
1.000000
2.000
Peak Location
Retention Tine (Bins): Relative Retention Tine: Tine Window (Bins): *
5.732, Enabled 0.000, Disabled 1.189
Peak Selection:
Yearest
Response
Response Type: Response:
External Areas
Calibration Curves
Polynomial Type: Point of Origin: Fit Weighting: Axis Transformation:
Linear Exclude Kona Yon*
Concentration
Units:
Noia*
P*ak~to~p*ak amplitude:
290
Smooth
Smoothing: Window size (scans):. Number of smooths: Smoothing method:
Disabled
3
2
Baselines
Join valley threshold: Reduce peak tailing: Raise baseline Baxiaum: '
0 .0 0 100.00 0.00
Peak Separation
Drew vertical three] ..
10.00
Detact shoulders:
* Disabled
Shoulder threshold:
40.00
Response Threshold
Relative height: Absolute height: Reletive area: Absolute area:
1.50, Disabled
0.00, Disabled
2.00,
'Disabled
80.00, Enabled
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Page 2
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Mathod Raport
'
Mathod fila: Last Bodifiad:
M:\rats.FRO\HathDB\030199c.adb Mon ltar 08 10:03:52 1999Q
'
Copound:
3:
Printed:
Compound Kim :
Internal Ref.: Quantify Trace: Cone, of Standards:
[None] TIC Cone. A
' m J>L
User Kf Value: User Peak Factor: Integrate Window Factor:
1.000000, Disabled
1.000000
2.000
Peak Location
Ratantion Tima tains):
Ralmtiva Ratantion Tiaa:
Tiaa Window (nins):
1.183, Vnabled 0.000, Disabled
1.000
Faak Salaction:
Response
Response Type: Response:
Nearest
Xxtemal Areas
Calibration Curves
Polynomial Type: Point of Origin: Fit Weighting: Axis Transformation:
Linear Xsr.lurie None None
Concentration
Units:
*
Noise
Faak-to-paak amplitude: Smooth
169
Smoothing: Window size (scans): Number of smooths: * Smoothing method:
.
Disabled 3 2
Baselines
Join valley threshold: Reduce peak tailing:
*Raise baseline maximum:
2100.00.000
0.00
Faak Senaraticn
Draw vertical thresholds Detect shoulders: Shoulder threshold:
*RasDOnsa Threshold
10.00 Disabled
0.00
Relative height: Absolute height:
Relative area: Absolute area:
1.50, Disabled 0.00, Dlaablad 2.00, -Dlaablad 00.00, Rnablad
.
cmm
I
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H.thod R.port
M.thod ill.: Last nodifi.d:
Compound:
Prlntd:
M:\rats.PR0\H.thDB\030199o.adb Mbit H u 08 10:03152 1999D
1: M M
Mon M*r 08 10:80:02 1 9
Compound Kane: Internal Ref.: Quantify Trace: Cone, of Standards:
PQAA [None] . TIC Cone. A
Uaar HP Valua:
Uaar Paak factor:
Intagrata Window Factor:
1.000000, Sisabl 1.000000 2.000
Peak Location
Retention Time (mins): Relative Retention Time: Time Window (mins): *
6.628, Xnablad 0.000, Disabled 1.000
Psak Selection:
Nearest
Response '
Response Type: Response:
Kxtemal Areas
Calibration Curves
Polynomial Type: Point of Origin: Pit Weighting: Axis Transformation:
Linear Exclude None None
Concentration
Units:
Noise
Peak-to-peak amplitude:
187
Smooth
Smoothing: Window size (scans): . Number of smooths: Smoothing method:
Disabltd 3 2
Baselines
Join valley threshold: Reduce peak tailing:. Raisa basalina maximum:.*
20.00 100.00 0.00
| n k Separation
Draw vertical threshold: Detect shoulders: Shoulder threshold:
10.00 * Disabled
. 40.00
Response Threshold
Relative height: Absolute height: Relative area: Absolute area:
1.5*0, Disabled
'0.00, Disabled
2.00,
'Disabled
80.00, Disabled
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Pag. 8
HarkL. m P M
l A c m M n ' 2___________
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Method Report
Method file: Last aodifiad: Coepound:
Printed:
M:\rata.PRO\MethDB\030199e.adb Mon Mar 00 10:03:32 19990
3: TelnertM
Mon Mar 00 10:10:02 1999
Compound Nan*: Internal Rtf.: Quantify Traca: Cene, of Standards:
,
Talaart69 [Kona]
TIC Cene. A
.
Usar II Vala:
Usar Peak Factor:
Integrate Window Factor:
1.000000, Disabled
1.000000
2 .0 0 0
Peak location
Retention Tine (nina): Relative Retention Tine: Tina Window (Bins): '
0.370, Inabled
0.000, Disabled
1.000
Peak Selection:
Nearest
Response
Response Types Responses
` *.
Sxternal Areas
Calibration Curves
Polynomial Types Point of Origins Fit Weighting: Axis Transformation:
Linear Kxelude None None
Concentration
Units:
Noise
Peak-to-peak amplitude:
169
Smooth
Smoothing: Window size (scans):. Number of smooths: Smoothing method:
Disabled 3 2
Baselines
Join valley threshold: Reduce peak tailing: Raise baseline maximum:.*
20.00 100.00 0.00
Peek Seoeratien
Drew vertical thresholds Detect shoulders: Shoulder threshold:
10.00 * Disabled
_ 40.00
Response Threshold
Relative height: Absolute height: Relative area: Absolute area:
1.30, Disabled 0.00, Dieabled 2.00,' Disabled 10.00, Inabled
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M i n i. OHlVL lACs t o U t f
Page 3
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Method Report
Method file; Last Modified:
Compound:
Printed:
-
M:\rets.PRQ\M.thDB\03al99c.db Mon Mar 08 10:03:52 19990
6: TelaerS69
Hon Mar 08 10:10:02 1999
Compound Kama: Internal Ref.: Quantify Traea: Cone, of Standard:
Talaar569 [Hone] TIC Cone. A
User RF Value: User Peak factor: Integrate Window factor:
Peak Location
1.000000, Disabled 1.000000 2.000
Retention Tiae (sdns): Relative Retention Tine: Tine Window (sins):
9.(18, Knebled 0.000, Disebled 0.500
Peak Selection:
Xearest
Response
Response Typo: Response:
#
External Areas
Calibration Curves
Polynomial Typa: Point of Origin: Fit Weighting: Axis Transformation:
Linear Sxclude Hone Hone
Concantration
Units:
*
Hoisa Peak-to-peak amplitude:
, 169
Smooth
Smoothings Window size (scans): Humber of smooths: * Smoothing method:
Disabled 3 2
e
Basalinas .*
Join valley threshold: Reduce peak tailing: Raise baseline neslwin:
20.00 100.00 0.00
Peak Separation
Draw vertical thresholds Detect shoulders: Shoulder threshold:
10.00 t Disabled
40.00
Response Threshold
Relative height: Absolute height: Relative area: Absolute area:
1.50, Disabled 0.00, Disebled 2.00,.Disabled 00.00, Inabled
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Page 6
__CHUM IO Qllot fa l
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Method Report Mathod fila: Last modiflad:
Coapound:
Printed:
H:\rats.PRO\MethDB\030199c.ndb M n t t a r O I 10:03:52 19*90
7: surr
Man Mar 00 10:10:02 1999
Compound Mama: Internal Kaf.t Quantify Traca: Cone, of Standards:
surr plana] TIC Fiaod
Umar RF Valua: Usax Paalc Factor: ' Xntagrata Window Factors
1.000000, Disabl 1.000000 2.000
Paalc Location
Retention line (mins): Relative Retention Tina: Tine Window (nine):
Peak Selection:
7.300, Inablad 0.00, Disabled 1.000
Rearest
Rasoonsa
Rasponsa Typas Rasponsa:
Xxtarnal Arm
Calibration Curvas
Polynomial Type: Point of Origin: Pit Weighting: Axis Tran:formation:
T.tnsar Xxcluda
Mona Mona
Concantration
Units:
*
Kolas
Paak-to-paalc amplituda:
169
Smooth
Smoothing: Window lira (scans): Nunbar of smooths: ' Smoothing mathod:
Disabled
3 2
Basalinas
Join valley threshold: Reduce peak tailing: Raise baseline nasiana:
Paalc Saturation
Drew vertical threshold: Detect shoulders:
Shoulder threshold:
,
20.00 100.00 0.00
10. Disabled 10.00
Response Threshold '
Ralativa haight: Absoluta haight: Ralativa araa: Absoluta araa:
1.50, Disabled 0.00, Disabled 2.00,. Disabled 00.00, Inabled
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11
` M aA elu. OMHM IAL D l i b s m
Paga 7 *
AMELIA062498: FACT Equipment Log 98061
Q uattro H , #6448E
PAGE
DATE: 03/16/99
fas
ANALYST: hoi STUDY NUMBER tox!20.
f MATRIX: R at liver
DESCRIPTION:
ion pair dilutions
FACT M ethod: FACT-M-4.0
Injection vftlm ne.10 uL
Cycle tim e: 13.5 m in.
M obile Phase:
(A)2.0 mM NH4CH3CQ2
TN-A- or stndrd num ber (A ) 98094-15-20________
Prepared (date/by):
(A) 3/16/99 hoj_________
Expires:
, (A) 09/99______________
Store at room tem p? Q o r N
O ther:
Electrode: Z-Spray ffilM eO H (B) TN-A-2325 (B) 3/16/99 hoi
(B) 9/16/99
G u ard colum n: N one__________
P art num ber na_________
Lot number na
A nalytical colum n: keystone bctasil__________
Size: 2 x 100mm________________ Type: c!8
Sphere size: 5 um
Part num ber 105-701-2
L ot nnmher P8HQ8 Serial num ber 029495D
A nalytical param eters:
Drying gas (L/hr): 300
ESI nebulizing gas fL/hr) 15
LC Flow rate: 300 uL/mm. Bade pressure: 100 bar
Sheath gas (L/hr)_0_
H PLC solvent gradient: long!
Tim e (m in.)
1) 0.00
U ! _____ 3 11.0 5) 12:5 ' 6 ) 13.5
%B
40
90
90
40
40
BACK TO MAIN
1 ^ ?Ufa_______ ;______________
Tuning Method Report 1
Method:
C :\MASSLYNX\RATS2. PRO\ACQUDB\PFOS
1 Printed:
Tue Mar 16 09:59:43 1999
___ ___________________________________ __________________
.)
Afrdia O L M W
BACK TO MAIN
Page 1
SOURCE ( ESP- )
] Capillary
Cone
.
J Extractor RF Lens Source Block Temp.
Desolvation Temp.
1
] Pressures
*
Analyser Vacuum
1 Gas Cell
Set Rdbk
3.00 60 5 0.2 150 .250
2.99 -60
-6
150 258
Rdbk
1.0e-4 4.5e-3
MSI
LM Resolution HM Resolution Ion Energy Ion Energy Ramp
Lens 6
Multiplier
MS2
LM Resolution HM Resolution Collision Ion Energy Ion Energy Ramp Lens 8 Lens 9 Multiplier
Set Rdbk
15.0 15.0 2.0 1 . 0.0 56 650
Set Rdbk
15.0 15.0 45 2.0 0.0 40 0 650
46
38 46
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ik k _ _ _ _ _ _ _ _ 1 I _ _ _ _ _ _ =_ _
Scanning Nathed Raporfe
Mathedi
C:\KXSSmOC\BXIM. PRO\ACOUDB\PFOS-PrHS-IS
Last Modified: Mon Mar IS 13:23:33 1*9
Printad:
Tua Mas IS 10:53:91 1999
Amila
M b os
Solvant Balay ( aina ) :
0.00
punetlon : 1
M M of 4 Stesa Paisa iI ESP- ) '
Intar Channal Daisy ( Sacs ) i
Span ( Daltons ) : Start Tlaa ( Kina ) :
End T i m ( Kina ) i
Rapaata :
Channal Parant Daughtar
0.03 0.00 0.00 13.SO 1 Duali (Saca) Cell Enargy (eV)
Cone C v )
1
499.00 80.00
0.40
45
60
2
499.00 99.00
0.40
4S
60
3 499.00 130.00 0.40
45
60
4 499.00 180.00 0.40 45 60
Function : 2
M M of 4 Masa Paisa iC ESP- )
Intar Channal Daisy 1 Saca ) t Span ( Daltons ) : Start Tina I Mins 1 t End Tina ( Hina ) : Rapaata :
. Channal Parant Daughtar
0.03 0.00 0.00 13.50
1 Duali
.
(Saca) Coll Enargy (eV)
* Cena ( V )
1
399.00 *80.00
0.40
2
399.00 99.00
0.40
3 399.00 130.00 0.40
4 399.00 180.00 0.40
45 45 45 45
60 60 60 60
:_ _ i _ Paga 1
function s 9
M M of 1 N u i Pais 1 ESP- )
.
Intar Channel Delay ( Seca ) i
Span ( Daltons ) :
Start Tina ( Mina ) : End Tine ( Hina ) :
Rapaata :
.
Channel Parent Daughtar
0.03 0.00 0.00 13.50
1 Dwell
(Saca) Coll Enargy (aV)
Cone ( V )
1
427.00 80.00
0.40
40
60
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3 It fa r____________ .________________________ __________________ ,_____________ _1
Masslynx - Sample List
Page 1
Sample List C:\MASSLYNX\rats2.PRO\SampleDB\031699b.SPL , Printed:TueM ar 1614:40:111S99______________________ I m L
D I.M J U it
/,
File Name
File Text
MS R ie
Inlet File
B o ttle
1 031699001
MeOH Blank
PFOS-PFHS-IS long2
91
2 031699002
MeOH Blank
PFOS-PFHS-IS long2
91
3 031699003
Tox120, RBL02229-H2OBIk-2,2/22/99
PFOS-PFHS-IS k>ng2
1
4 031699004
Tox120, RBl02229-UvBlk-2,2/22/99
PFOS-PFHS-IS long2
2
5 031699005
Tox120, RBL02229-5ppb-2,2/22/99
PFOS-PFHS-IS long2
3
6 031699006
Tox120, RBL02229-10ppb-2,2/22/99
PFOS-PFHS-IS long2
4
7 031699007
Tox120, RBL02229-25ppb-2,2/22/99
PFOS-PFHS-IS long2
5
8 031699008
Tox120, RBL02229-50ppb-2,2/22/99
PFOS-PFHS-IS long2
6
9 031699009
Tox120, RBL02229-100ppb-2,2/22/99
PFOS-PFHS-IS bng2
7
10 031699010
TOX120, RBL02229-250ppb-2,2/22/99
PFOS-PFHS-IS long2
8
11 031699011
Tox120. RBL02229-500ppb-2,2/22/99
PFOS-PFHS-IS long2
9
12 031699012
Tox120, RBL02229-750ppb-2,2/22/99
PFOS-PFHS-IS tong2
10
13 031699013
Tox120, RBL02229-1000ppb-2,2/22/99
PFOS-PFHS-IS long2
11
14 031699014
MeOH Blank
PFOS-PFHS-IS long2
91
15 031699015
Tox120,161F-G 5,2/22/99, d250
PFOS-PFHS-IS long2
12
16 031699016
Tox120,162F-G5,2/22/99, d250
PFOS-PFHS-IS long2
13
17 031699017
Tox120,163F-G5,2/22/99, d250
PFOS-PFHS-IS long2
14
18 031699018
Tox120,164F-G5,2/22/99, d250
PFOS-PFHS-IS long2
15
19 031699019
TOX120,165F-G5,2/22/99, d250
PFOS-PFHS-IS long2
16
20 031699020
Tox120, RBL02229-250ppb-CC2,2/22/99 PFOS-PFHS-IS long2
82
21 031699021
MeOH Blank
PFOS-PFHS-IS long2
91
22 0316*99022
Tox120,166F-G5,2/22/99, d250
PFOS-PFHS-IS long2
17
23 031699023
Tox120,167F-G5,2/22/99, d250
PFOS-PFHS-IS long2
18
24 031699024
Tox120,168F-G5,2/22/99, d250
PFOS-PFHS-IS long2
19
25 031699025
Tox120, RBL02229-250ppb-CC3,2/22/99 PFOS-PFHS-IS long2
83
26 031699026
MeOH Blank
PFOS-PFHS-IS long2
92
27 031699027
Tox120,177F-G6,2/22/99, d1000 .
PFOS-PFHS-IS long2
20
28 031699028
Tox120,1787F-G6,2/22/99, d1000
PFOS-PFHS-IS long2
21
29 031699029
Tox120,179F-G6,2/22/99, d1000
PFOS-PFHS-IS long2
22
30 031699030
Tox120,180F-G6,2/22/99, d1000
PFOS-PFHS-IS long2
23
31 031699031
TOX120,181F-G6,2/22/99, d1000
PFOS-PFHS-IS long2
24
32 031699032
TOX120, RBL02229-250ppb-CC4,2/22/99 PFOS-PFHS-IS long2
84
33 031699033
MeOH Blank
PFOS-PFHS-IS long2
92
34 031699034
Tox120,182F-G6,2/22/99, d1000
PFOS-PFHS-IS long2 .
25
35 031699035
TOX120,183F-G6,2/22/99, d1000
PFOS-PFHS-IS long2
26?
36 031699036
Tox120,184F-G6,2/22/99, d1000
PFOS-PFHS-IS long2
27
37 031699037
Tox120,185F-G6,2/22/99, d1000
PFOS-PFHS-IS long2
28
38 031699038
TOX120,186F-G6,2/22/99, d1000
PFOS-PFHS-IS long2
29
39 031699039
Tox120, RBL02229-250ppb-CC5,2/22/99 PFOS-PFHS-IS long2
85
40 031699040
MeOH Blank
PFOS-PFHS-IS long2
92
41 031699041
Tox120,187F-G6,2/22/99, d1000
PFOS-PFHS-IS long2
30
42 031699042
Tox120,189F-G6,2/22/99, d1000
PFOS-PFHS-IS. long2
31
43 031699043
TOX120,190F-G6,2/22/99, d1000
PFOS-PFHS-IS long2
32
44 031699044
Toxl20,191F-G 6,2/22/99, d1000
PFOS-PFHS-IS long2
33
45 031699045 Tox120, RBL02229-250ppb-CC6,2/22/99 PFOS-PFHS-IS long2
86
46 031699046
MeOH Blank
PFOS-PFHS-IS long2
93
47 031699047
Tox120.169F-G5,2/22/99 .
PFOS-PFHS-IS long2
34
48 031699048
T o x1 20 ,1 70 F -G 5 ,2/22/99
PFOS-PFHS-IS long2
35
49 031699049
T o x1 20 .1 71 F -G 5 ,2/22/99
PFOS-PFHS-IS long2
36
50 031699050
Tox120, RBL02229-250ppb-CC5, 2/22/99 PFOS-PFHS-IS fong2
85
51 031699051
MeOH Blank
PFOS-PFHS-IS long2
94
52 031699052
T o x1 20 .1 73 F -G 5 ,2/22/99
PFOS-PFHS-IS long2
37
53 031699053
T o x1 20 .1 74 F -G 5 ,2/22/99
PFOS-PFHS-IS fong2
38
54 031699054
T o x1 20 .1 75 F -G 5 ,2/22/99
PFOS-PFHS-IS bng2
39
55 031699055
Tox120, RBL02229-250ppb-CC6,2/22/99 PFOS-PFHS-IS bng2
86
56 031699056
MeOH Blank
PFOS-PFHS-IS bng2
94
57 031699057
Tox120, RBL02229-H2OBIk-2,2/22/99
PFOS-PFHS-IS bng2
1
58 031699058
Tox120, RBL02229-LivBlk-2,2/22/99
PFOS-PFHS-IS bng2
2
59 031699059
*Tox120, RBL02229-5ppb-2,2/22/99
PFOS-PFHS-IS long2
3
60 031699060
Tox120, RBL02229-1Oppb-2,2/22/99
PFOS-PFHS-IS long2
4
Volume Sam
10 Anal 10 Anal 10 Anal 10 Anal 10 Stan 10 Stan 10 Stan 10 Stan 10 Stan 10 Stan 10 Stan 10 Stan . 10 Stan 10 Anal 10 Anal 10 Anal 10 Anal 10 Anal 10 Anal 10 QC 10 . Anal 10 Anal 10 Anal 10 Anal 10 QC 10 Ana; . 10 Ana 10 Ana 10 Ana 10 Ana 10 Ana 10 QC 10 Ana . ' 10 Ana 10 Ana 10 Ana 10 Ana 10 Ana
10 QC 10 Ana 10 Ana 10 Ana 10 Ana 10 Ana 10 QC 10 Ana 10 Ana 10 Are 10 Ana 10 QC 10 Are 10 Are 10 Are 10 Are 10 QC 10 Are
10 Are 10 An; 10 Sta 10 Sta
BACK TO MAIN
ta\ 3ilefa___;_____ ;______________ __________;_________
Masslynx - Sample List
' Page 2
Sample U s t C:\MASSLYNX\ratsZPRO\SampleDB\Q31699b.SPL
Printed: Tue Mar 1614:40:11 1999
\hwliR 0I*2W
IAC-0s/lolll
File Name
61 031699061 62 031699062 63 031699063 64 031699064 65 031699065 66 031699066 67 031699067
File Text
.
TOX120, RBL02229-25ppb-Z2/22/99 Tox120, RBL02229-50ppb-2,2/22/99
Tox120, RBL02229-100ppb-2,2/22/99 Tox120, RBL02229-250ppb-2,2/22/99 Tox120, RBL02229-500ppb-2,202/99
Tox120, RBL02229-750ppb-Z 2/22/99 Tox120, RBL02229-1000ppb-2,2/22/99
MS File
PFOS-PFHS-IS PFOS-PFHS-IS PFOS-PFHS-IS PFOS-PFHS-IS PFOS-PFHS-IS PFOS-PFHS-IS PFOS-PFHS-IS
Inlet File
long2 k>ng2 k>ng2 long2 k>ng2 long2 k>ng2
Bottle
5 6 7 8 9 10 11
Volume Sam
10 Stan 10 Stan 10 Stan 10 Stan 10 Stan 10 Stan 10 Stan
I
FACT: DATA REVIEW SUMMARY FORM
BACK TO MAIN
Date: 3/17 Iw
1
J
I
BACK TO MAIN
Quantify Compound Sunnary Raport T0X120 Sat Livor Group 3 and
Sanpla Llat: G:\rata2.FBO\8aqplaDB\Q31699b La at nodifind: Wod klar 17 13:54:10 1999
Mathod:
G:\rata2.PRO\MathDB\03l699b
Last modiflad: Wad Mar 17 06:56:20 1999
Job Coda:
.
Printad:
Wad Mar 17 14:33:58 1999
Compound 1: PTOS
Mama 1 031699001 2 031699002 3 031699003 4 031699004 5 031699005 6 031699006 7 031699007 031699008 9 031699009 10 031699010 11 031699011 12 031699012 13 031699013 14 031699014 15 031699015 16 031699016 17 031699017 ia 031699018 19 031699019 20 031699020 21 031699021 22 031699022 23 031699023 24 031699024 25 031699025 26 031699026 27 031699027 2S 031699028 29 031699029 30 031699030 31 031699031 32 031699032 33 031699033 34 031699034 35 031699035 36 031699036 37 031699037 38 031699036 39 031699039 40 031699040 41 031699041 42 031699042 43 031699043 44 031699044 45 031699045 46 031699046 47 031699047 48 031699048 49 031699049 SO 031699050 51 031699051 52 031699052 53 031699053 54 031699054 55 031699055 56 031699056 ' 57 031699057 58 031699058 59 031699059 60 031699060 61 031699061 62 031699062 63 031699063 64 031699064 65 031699065 66 031699066 67 031699067
luvlaliit Txt
K.OH Blank
MaOH Blank
Toxl20, WL0222S-H20BU-2, 2/22/99
Toxl20, KBL02229-LiYBlk-2, 2/22/99
Texl20, BBL02229-Sppb-2, 2/22/99
TOX120, ML02229-10ppb-2, 2/22/99
TOX120, RBL02229-25ppb-2, 2/22/99
Toxl20, ML02229-50ppb-2, 2/22/99
Toxl20, RBZ.02229-100ppb-2, 2/22/99
Toxl20, RBL02229-250ppb-2, 2/22/99
Toxl20,RBL02229-500ppb-2, 2/22/99
TOX120, ABL02229-750ppb-2, 2/22/99
TOX120, ABL02229-1000ppb-2, 2/22/99
MaOK Blank
.
Toxl20, Kir-GS, 2/22/99, d2S0
TOX120, 1S2F-GS, 2/22/99, -d250 '
TOX120, 1S3F-G5, 2/22/99, d2S0
Toxl20, 16<r-GS, 2/22/99, d2S0
Toxl20, 16SF-GS, 2/22/99, d250
TOX120, BBLO2229-2S0ppb-CC2, 2/22/99
MaOH Blank
TOX120, 1C6F-G5, 2/22/99, <1250
Toxl20, m r - C S , 2/22/99, d250
Toxl20, l(ar-6S, 2/22/99, d250
Toxl20,*RBL02229-2S0ppb-CC3, 2/22/99
MaOH Blank 0
Toxl20, 177r-GS, 2/22/99, dlOOO
TOX120, 170XMS, 2/22/99, dlOOO
Toxl20, 179r-0S, 2/22/99, dlOOO
Toxi20, aor-eo, 2/22/99, diooo
Toxl20, 1I1F-G6, 2/22/99, dlOOO
TOX120, BBL02229-250ppb-CC4, 2/22/99
MaOH Blank .
TOX120, 192r-G5, 2/22/99, dlOOO
TOX120, 183F-GS, 2/22/99, dlOOO
Toxl20, 1S4F-G6, 2/22/99, dlOOO
TOX120, 195F-GS, 2/22/99, dlOOO
TOX120, 196F-G6, 2/22/99, dlOOO
Toxl20, BBL02229-250ppb-CCS, 2/22/99
MaOH Blank
TOX120, 197F-C6, 2/22/99, dlOOO
TOX120, 199F-66, 2/22/99, dlOOO
Toxl20, 190F-GC, 2/22/99, dlOOO
TOX120, 191F-66, 2/22/99, dlOOO
TOX120, BBL02229-250ppb-CC6, 2/22/99
MaOH Blank
TOX120. 1S9F-G3, 2/22/99
TOX120. 170F-G5, 2/22/99
Toxl20..171F-GS, 2/22/99
Toxl20,' RBL02229-250ppb-CC5, 2/22/99
MaOH Blank
TOX120. 173F-6S, 2/22/99
Toxl20. 174F-GS, 2/22/99 IwliO. 175F-GS, 2/22/99
TOX120, RBlO2229-2S0ppb-CC, 2/22/99
MaOH Blank .
.
Toxl20, BBL02229-H20Blk-2, 2/22/99
TOX120, m 0 2 2 2 9 - U v B l k - 2 , 2/22/99
TOX120, RBIi02229--5ppb-2, 2/22/99
TOX120, BBL02229-10ppb-2, 2/22/9
TOX120, BBL02229-25ppl>-2, 2/22/99
Toxl20, BBL02229-50ppb-2, 2/22/99
TOX120, RBL02229-100ppb-2, 2/22/99
Toxl20, m02229-250ppb-2, 2/22/99
TOX120, RB02229-500ppb-2, 2/22/99
TOX120, HBL02229-750ppb-2, 2/22/99
TOX120, ABl*02229-1000ppb-2, 2/22/99
.
.
.
Pillila .
Fypa Analy Analy Analy Analy Stand Stand Stand Stand Stand Stand Stand Stand Stand
. Analy Analy Analy Analy Analy Analy QC
Analy Analy Analy Analy QC Analy Analy Analy Analy Analy Analy QC Analy Analy . Analy Analy Analy Analy QC' Analy Analy Analy Analy Analy QC Analy Analy Analy Analy QC Analy Analy Analy Analy QC Analy Analy Analy Stand Stand Stand Stand Stand Stand Stand Stand Stand
8td Cone
5.99 12.00 29.90 59.90 120.00 299.00 599.00 898.00 1198.00
299.00
299.00
299.00
299.00
299.00
ST 8.474 9.067 9.026 9.096 9.084 9.033 9.020 8.969 9.029 9.033 9.036 9.034 8.977 8.973 9.103 *
9.060 9.114 9.101 9.058 . 9.070 9.066 9.070 9.068 9.063 8.805 9.066 9.057 9.065 9.060 9.057 9.061 9.056 9.064 9.056 9.051 9.056 9.005 9.062 9.001 9.075 9.032 9.085 9.040 9.03S
At m 8749
114 318 378 777 1043 1973 3368 6297 15755 29424 39984 62763 128 253
65 96 128 9673 243 165 262 45 12476 28 18135 18935 19227 18644 20438 11041 45 19755 16765 16092 10923 13246 11816 155 11727 9219 9388 10457 10389
9.031 8.907 8.914 299.00 8.907 8.976
8.975 8.920 .918 299.00 8.908 9.045 1.977 8.971 5.99 8.968 12.00 8.898 29.90 8.967 59.90 8.907 120.00 8.969 299.00 8.910 599.00 8.900 898.00 8.918 1198.00 8.971
3257 3363 2407 13894
121 3473 2677 5396 12660
49 304 329 768 1172 X 1988 3354 6672 15526 29963 43816 62400
Saaponaa 8749.32 114.33 317.81 378.28 777.34 1043.01 1973.20 3368.08 6297.05
15754.60 29423.97 39983.57 62762.84
128.18 253.39
64.63 95.67 128.38 ' 9673.44 242.96 . 164.97 262.30 44.62 12476.30 28.42 18134.95 18935.20 19226.52 18644.16 20437.57 11040.82 45.17 19754.86 16764.61 16091.88 10923.35 13245.65 11815.78 155.06 11727.19 9219.46 9388.04 10457.05 10389.20
3257.20 3362.65 2407.33 13893.83
120.93 3473.23 2677.29 5396.21 12660.21
49.26 303.77 328.82 767.69 1172.49 1988.14 3353.64 6672.19 15526.06 29962.79 43815.88 62399.96
Cono. Plaaa tfiav
172.09 bb
0.00 bb
0.00 bb
0.00 bb
5.39 MM
-10
10.94 bb 30.39 bb
-9 2
59.56 bb
-1
120.81 bb
1
318.57 bb 604.41 MM
7 1
825.22 bb
-8
1301.55 bbX
9
0.00 HM
0.00 bb
0.00 bb 0.00 bb 0.00 bb 191.41 MM 0.00 bb 0.00 bb 0.00 bb 0.00 bb 250.02 bb 0.00 bb 368.35 MM 385.08 bb 391.17 bb 379.00 MM 416.50 MM 220.00 KM 0.00 bb 402.22 MM 339.69 Mi 325.63 bb 217.55 MM 266.11 bb 236.21 bb 0.00 bb 234.36 bb 181.92 MM 185.44 KM 207.80 MM 206.38 MM
. -36 -16 \ -26
-21
-31
57.24 bb 59.45 bb ,, 39.47 bb 279.66 bb * 0.00 bb 61.76 bb 45.12 bb 101.97 bb 253.87 bb
0.00 bb ' 0.00 bb
0.00 bb 5.19 Ml 13.65 bb 30.71 bb 59.26 bb 128.65 bb
313.79 bb 615.68 bb 905.35 bb 1293.96 bbX
-6
-15
-13 14 3 -1 7 5 3 1 8
Amalia 052498 quanti tatad and printad on 03/17/99 by LAC
BACK TO MAIN
1
!
]
I
]
1
1
J
1 ] 1
Quantify Ceo^sund Suaaacy M p o r t 10X120 Rat 1*1var Group S and <
Sanpl. List! O:\xats2.VRO\Iug>lDBV)31i9b
Last andlflad: ad Mac IT 13;S4:10 1*9#
Nathod:
G:\cata2.PRO\MathDB\031CMb
Last aodiflad! Wad Mac IT 0:S:20 1
Job Codas
Printad:
ad Mac IT 14i33i5( 1999
Compound 2i tucc
t Mana 1 031699001 2 031699002 3 031699003 4 031699004 S 031699005 6 031699006 7 031699007 031699008 9 031699009 10 031699010 11 031699011 12 031699012 13 031699013 14 031699014 IS 031699015 16 031699016 17 031699017 18 031699018 19 031699019 20 031699020 21 031699021 22 031699022 23 031699023 24 031699024 25 031699025 26 031699026 27 031699027 28 031699028
29 031699029 30 031699030
31 031699031 32 031699032 33 031699033
34 031699034 35 031699035 36 031699036 37 031699037
38 031699038 39 031699039 40 03169904Q 41 031699041 42 031699042 43 031699043 44 03169904-4 45 031699045 46 031699046 47 031699047 48 031699048 49 031699049 50 031699050 51 031699051 52 031699052 53 031699053 54 031699054 55 031699055 56 031699056 ` 57 031699057 58 031699058 59 031699059 60 031699060 61 031699061 62 031699062 63 031699063 64 031699064 65 031699065 66 031699066 67 031699067
Saaplallat Taxt
HsOR Blank
MaOK Blank
Toxl20, BBL02229-H20Blk-2, 2/22/99
Toxl20, RBL02229-UvBlk-2, 2/22/99
Toxl20, ABL02229-Sppb-2, 2/22/99
TOX120, RBL02229-10ppb-2. 2/22/99
Toxl20, RBL02229-2Sppb-2, 2/22/99
TOX120, RBL02229-50ppb-2, 2/22/99
TOX120, RBL02229-100ppb-2, 2/22/99
TOX120, BBLO2229-2S0ppb-2, 2/22/99
TOX120/RBL02229-500ppb-2, 2/22/99.
Toxl20, RBL02229-T50ppb-2, 2/22/99
TOX120, KBL02229-1000ppb-2, 2/22/99
HaCH Blank
TOX120, 161F-G5, 2/22/99, d250 .
Toxl20, K2F-GS, 2/22/99, -d2S0
TOX120, 1S3F-GS, 2/22/99, d250
Toxl20, 164F-G5, 2/22/99, d250
TOX120, 165F-GS, 2/22/99, 0250
TOX120, R8L02229-2Sppb-CC2, 2/22/99
MaOH Blank
.
Toxl20, 1C9F-CS, 2/22/99, d250
TOX120, 1CTF-G5, 2/22/99, d2S0
Toxl20, 1SSF-G5, 2/22/99, (1250
TOX120, RBL02229-2S0ppb-CC3, 2/22/99
MaOH Blank p
TOX120, 1TTF-G, 2/22/99, dlOOO
Toxl20, 17B/F-G, 2/22/99, dlOOO
TOX120, 1T9F-G6, 2/22/99, dlOOO
TOX120, 180F-G6, 2/22/99, dlOOO
Toxl20, liir-GC, 2/22/99, dlOOO
'
TOX120, RBL02229-250ppb-CC4, 2/22/99
MaOH Blank
TOX120, 152r-GC, 2/22/99, dlOOO
TOX120, 183F-G6, 2/22/99, dlOOO
TOX120, 184F-G6, 2/22/99, dlOOO
TOX120, 18SF-G8, 2/22/99, dlOOO
TOX120, 186r-G6, 2/22/99, dlOOO
TOX120, RBL02229-250ppb-CCS, 2/22/99
MaOH Blank
TOX120, 18TF-G, 2/22/99, dlOOO
TOX120, 189F-G6, 2/22/99, dlOOO
TOX120, 190F-G8, 2/22/99, dlOOO
Toxl20, 191F-G6, 2/22/99, dlOOO
TOX120, BBL02229-250ppb-CC8, 2/22/99
MaOH Blank
Toxl20. 169F-GS, 2/22/99
*
Toxl20. 170F-G5, 2/22/99
TOX120.-1T1F-GS, 2/22/99
TOX120,' RBL02229-2S0ppb-CCS, 2/22/99
MaOH Blank
TOX120. 1T3F-G5, 2/22/99
TOX120. 1T4F-G5, 2/22/99 TOX120. 1T5P-G5, 2/22/99
Toxl20, BBL02229-250ppb-CC6, 2/22/99
MaOH Blank
TOX120, RBL02229-H20Blk-2, 2/22/99
TOX120, RBL02229-LivBlk-2, 2/22/99
Toxl20, RBL02229-5ppb-2, 2/22/99
TOX120, RBL02229-10ppb-2, 2/22/99'
Toxl20, RBL02229-25ppb-2, 2/22/99
Toxl20, BBL02229-50ppb-2, 2/22/99
TOX120, BBL02229-100ppb-2, 2/22/99
TOX120, BBL02229-250ppb-2, 2/22/99
Toxl20, RBL02229-500ppb-2, 2/22/99
Toxl20, BBL02229-T50ppb*2, 2/22/99
TOX120, BBL02229-1000ppb-2, 2/22/99
( p ^ ) l A C 0^1 R H * !
' '
TVpa Analy Analy Analy Analy Stand Stand Stand Stand Stand Stand Stand Stand Stand Analy Analy ` Analy Analy Analy Analy . QC Analy Analy Analy Analy QC Analy Analy Analy Analy Analy Analy QC Analy Analy Analy Analy Analy Analy QC Analy Analy Analy Analy Analy QC Analy Analy Analy Analy QC Analy Analy Analy - Analy QC Analy Analy Analy Stand Stand Stand Stassd Stand . Stand Stand
Stand Stand
Std Cono
Kt
1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00
1.412
8.404 8.420 8.404 8.354 8.413 8.354 8.358 8.354 8.361 8.356
1.00
8.290 8.378
8.453
1.00
8.37*9 8.384 8.38^
8.123
1.00 8.380
7.984
1.00 8.446
1.00
1.00
1.00
1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00
8.355
8.418 B.228 8.235 8.294
8.361 8.303 8.301 8.230
8.359 8.359 8.290 8.285 8.289 8.289 8.357 8.295 8.286 8.304 8.292
' .
tag* 2 _
Aru
684
2274 2907 2500 2189 1931 . 2004 1623 1549 1775
48
8 pona*
684.42
2274.17 2906.68 2499.85 2189.27 1931.02 2004.23 1622.84 1548.68 1774.97
47.58
12 . 737
7.
12.14 736.68
7.06
1174 9
10 14
982
9
1173.91 8.97 9.59
14.36
. 981.80
9.15
955 955.41
Cono. llaga %Dv
0.41 bb
1.37 bb 1.75 bb 1.51 bb 1.32 bb 1.16 bb 1.21 bb 0198 bb 0.93 bb 1.07 bb 0.03 bb
-59
37 75 51 32 16 21 -2 -7
7 -97
0.01 bb 0.44 bb 0.00 bb
-99 -56 -100
0.71 bb 0.01 bb 0.01 bb 0.01 bb
0.59 bb
0.01 bb
-29 -99 -99 -99
-41
-99
0.58 bb -42
741
1213 601 617 809
1236 706 658 623
1229 1222 1139 , 1567 .X 1317 1190 1278 1211 1102 1179 1136
740.87
1213.32 600.53 616.93 809.04
1235.83 706.35 657.72 623.03
1229.39 1221.94 1138.70 1567.05 1317.43 1189.57 1277.63 1211.23 1102.30 1178.81 1135.66
0.45 bb
0,73 bb 0.36 bb * 0.37 bb # 0.49 bb
0.74 bb 0.43 bb 0.40 bb ' 0.38 bb
0.74 bb 0.74 bb 0.69 bb 0.94 bb 0.79 bb 0.72 bb 0.77 bb 0.73 bb 0.66 bb 0.71 bb 0.68 bb
-55
-27 * -64
-63 -51
-26 -57 -60 -62
-26 -26 -31
-6 -21 -28 -23 -27 -34 -29 -32
Anclla 062498 quantitated and printad on 03/17/99 by ZAC
1
Quantify Calibration Raport 90X120 Rat Livax Group 8 and 8
Calibration: G:\rata2.PRO\CurvaDB\031S99b
Last aodiflad: Wad Mar 17 14:37:10 1999
Printad:
Wad Mar 17 14:34:29 1999
.
* .
BACK TO MAIN
Pa 1 #
Quantify Calibration Raport TCX120 Rat Livar Group S and (
Calibration: O:\rata2. 9R0\CurvaDB\031C99b
Laat aodifiad: Wad Mar 17 14i37<10 199
Printad:
Wad Mar 17 14:34>29 1999
'
' '
' '
BACK TO MAIN
hga 3
Amalia 042499 quantittad and printad an 03/17/99 by LAC
1
Method Report
1 Method file: Last modified: Compound:
0: \rats2. PRO\MethDB\031699b.mdb Wed Mar 17 06:54:20 1999
1: PPOS
Printed:
Wed Mar 17 13:11:29 1999
1 Compound Name:
Internal Ref.:
Quantify Traca
Cone. o Standards
I Usar W Valas Usar Peak Faetor:
.
Integrate Window Faetor:
PPOS [None]
TIC Cone. A
1.000000f Disabled
1.000000
2 .0 0 0
.
A/nelio.
Retention Tina (mine) : Relative Retention Tina: Tine Window (nine) i
Peak Selections
] Response Types Response:
8.843, Enabled 0.000, Disabled 0.717
Nearest
External . Areas
1 Polynomial Type: Point of Origin: Fit Weighting: Axis Transformation:
7 Units:
Peek-to-peak amplitude:
Linear Exclude
1/X
None
293
Smoothings Window sise (scans) s Number of smooths: Smoothing method:
* Disabled
3
2
Join valley thresholds Reduce peak tailing: Raise baseline maxiana:
] Draw vertical threshold: Detect shoulders: Shoulder threshold:
J Relative heights Absolute heights Relative areas * Absolute areas
30.00 70.00 4.00
10.00 Disabled 10.00
50.00, Enabled 0.00. Disabled 3.00, Enabled 0.00, Disabled
BACK TO MAIN
* page :
_____ I L l l S ______________
Method Report
Method file: Last modified:
Compound:
print.di
a :\r.t,2.PXO\ltethDB\031699b.Mdb N.d M w 17 06:36*20 1003
2: Surr
Had Mar 17 11:11:29 1999
Compound Nam* t Internal Ref.: Quantify Traai Cone, of Standards{
Dear RF Values User Peak Factor: Integrate Window Factor:
Surr (Non] TIC Fixed
*
1 .0 0 0 0 0 0 , D isa b le d
1.000000
2 .0 0 0
Retention Time (mine):
1 Relative Retention Time: Time Window (mins) :.
Peak Selection:
3 Response Type: Response:
8.231, Enabled 0.000, Disabled
0.S88
Nearest
External . Areas '
Polynomial Type: Point of Origin: Fit Weighting: Axis Transformation:
T units:
Peak-to-peak asplitude:
\
i i
i Smoothing:
Window size (scans): Number of smooths:
i Smoothing method:
T.lnoar Exclude None None
n
Disabled
3
2
Join valley threshold: Reduce peak tailing:
t Raise baseline maximum:
30.00 70.00 4.00
]
DraW vertical threshold:
10.00
Detect shoulders:
. Disabled
Shoulder threshold:
J Relative height:
* * 10.00 50.00, Enabled
Absolute height:
0.00, Disabled
Relative area:*
3 .0 0 , BM bled
Absolute area:
0.00, Disabled
:.
A fo tL o t z w
BACK TO MAIN
Page :
___Ac o iln -jw
BACK TO MAIN
1
Quantify Cenpound Suaaary Kapert TOX120 Rat Llvar Qroup 5 and f
Vaga 1
1
Baapl. Liat: a:\rata2.Ht0\8anpla0B\031dRtb
Laat .edified: Wad Mar IT 13:54)10 19
Matbed:
O:\rata2.moVMathDB\031tttb2
laat aodlflad: Wad Mar IT 0(:5:0 1
Job Coda:
Printed: ' Wad Mar IT 15:01:13 1
Coapeund 1: PIHf
] ] 1 T
iI
J 1 ]
Ms m s
Saaplali.t Text
1 031699001 HeOH Blank
2 031699002 MaOH Blank
3 031699003 Toxl20, BBl02229-H2OBlk-2, 2/22/9
4 031699004 Toxl20, RBL02229-LlvBlk-2, 2/22/9
5 031699005 TOX120, RBU)2229-5ppb-2, 2/22/99
6 031699006 TOX120, R8L02229-10ppb-2, 2/22/99
7 031699007 TOX120, RBL02229-25ppb-2, 2/22/9
031699008 TOX120, RBL02229--50ppb-2, 2/22/99
9 031699009 Toxl20, RBL02229-- 100ppb-2, 2/22/99
10 031699010 TOX120, RBL02229-2S0ppb-2, 2/22/99
11 031699011 TOX120, -RBL02229-500ppb-2, 2/22/9
12 031699012 TOX120, RBL0222-TS0ppb-2, 2/22/99
13 031699013 TOX120, RBL02229-1000ppb-2, 2/22/99
14 031699014 MaOH Blank
15 031699015 TOX120, liir-GS, 2/22/9, d2S0
16 031699016 Toxl20, 192P-65, 2/22/9,.d250 '
17 031699017 Toxl20, lC3r-G5, 2/22/99, d2S0
It 031699018 TOX120, 14F-G5, 2/22/99, d2S0
19 031699019 Toxl20, KSF-GS, 2/22/99, d250
20 031699020 TOX120, RBL02229-250ppb-CC2, 2/22/99
21 031699021 MaOH Blank
22 031699022 TOX120, 166T-G5, 2/22/99, d250
23 031699023 TOX120, 16T1--G5, 2/22/99, d2S0
24 031699024 Toxl20, ltr-GS, 2/22/9, d2S0
25 031699025 TOX120, RBI.0222-250ppb-CC3, 2/22/99
26 931699026 MaOH Blank In
27 031699027 Toxl20, 1TTF-KSC, 2/22/99, dlOOO
26 031699028 Toxl20, 1T9VT-G6, 2/22/9, dlOOO
29 031699029 TOX120, 1T9F-G6, 2/22/99, dlOOO
30 031699030 Toxl20, 1S0F-GC, 2/22/9, dlOOO
31 031699031 Toxl20, 1S1F-GC, 2/22/9, dlOOO
32 031699032 TOX120, RBL02229-250ppb-CC4, 2/22/99
33 031699033 MaOH Blank
.
34 031699034 TOX120, 1S2F-66, 2/22/99, dlOOO
35 031699035 TOX120, 193F-S4, 2/22/99, dlOOO
36 031699036 Toxl20, 194F-GS, 2/22/9, dlOOO
37 031699037 TOX120, 19SF-GC, 2/22/9, dlOOO
38 031699038 Ioxl20, 1IGF-GC, 2/22/9, dlOOO
39 031699039 Toxl20, RBL02229-250ppb-CC5, 2/22/9
40 031699040 MaOH Blank
41 031699041 TOX120, 1ITF-G6, 2/22/99, dlOOO
42 031699042 -TOX120, 199F-GS, 2/22/99, dlOOO
43 031699043 TOX120, 190F-GC, 2/22/99, dlOOO
44 031699044 TOX120, 191F-G6, 2/22/9, dlOOO
45 031699045 Toxl20, BB1.02229-2S0ppb-CCS, 2/22/99
46 031699046 MaOH Blank
47 031699047 TOX120. 1C9F-G5, 2/22/9
48 031699048 TOX120. 1T0F-G3, 2/22/9
49 031699049 TOX120. .niF-GS, 2/22/99
50 031699050 Toxl20,-RBL02229-250ppb-CC5, 2/22/99
51 031699051 MaOH Blank
52 031699052
53 031699053 54 031699054 55 031699055
56 031699056 *
57 031699057
TOX120. 1T3F-GS, 2/22/9 TOX120. 174F-GS, 2/22/9 TOX120. 17SF-GS, 2/22/9 TOX120, RBL02229-250ppb-CCC, 2/22/99
MaOH Blank TOX120, RBL02229-H2QBlk~2, 2/22/99
58 031699058 TOX120, RBL02229-LivBlk-2, 2/22/9
59 031699059 TOX120, RB102229-5ppb-2, 2/22/99
60 031699060 Toxl20, BBL02229-10ppb-2, 2/22/9'
61 031699061 62 031699062
TOX120, RBL02229-2Sppb-2, 2/22/9 TOX120, RBL02229-S0ppb-2, 2/22/99
63 031699063 TOX120, RBL0222-100ppb-2, 2/22/99
64 031699064 Toxl20, RBL02229-250ppb-2, 2/22/99
65 031699065 Toxl20, RBS02229-S00ppb-2, 2/22/99
66 031699066 67 031699067
TOX120, RBL02229-T30ppb-2, 2/22/99 TOX120, RBL02229-1000ppb-2, 2/22/99
G > i A t oijiahi
'
'
tP4 . Analy Rnaly Analy Analy . Stand ' Stand Stand Stand Stand Stand Stand Stand Stand Analy Analy Analy Analy Analy Analy QC Analy Analy Analy Analy QC Analy Analy Analy Analy Analy Analy QC Analy Analy Analy Analy Analy Analy QC' Analy Analy Analy Analy Analy QC Analy Analy Analy Analy QC Analy Analy Analy Analy QC Analy Analy Analy Stand Stand Stand Stand Stand 'Stand Stand Stand Stand
Std Cono
5.99 12.00 29.90 59.90 120.00 299.00 599.00 898.00 1198.00
299.00
299.00 *
299.00
299.00
299.00
299.00
299.00
5.99 12.00 29.90 59.90 120.00 299.00 599.00 898.00 1198.00
Kt
7.878 7.829 7.812 7.828 7.820 7.826 7.830 7.826 7.832
7.830 *7.893
7.849 7.901 7.889 7.850
7.857 7.793 7.857 7.850
*
7.849
7.851
7.827
7.889 7.766 7.770 7.702
7.831 7.775 7.711 7.765
7.761 7.757 7.762 7.763 7.766 7.770 7.759 7.775 7.766
At m
52 93 216 325 751 1966 ' 3677 5126 7909
2007 2569 2200 2150 2715 2299
2120 2652 1786 2773
I960
2185
' 2065
2303 999
1350 1319
234 175 203 1298
46 99 178 328 730 1710 3403 5157 7163
Response
Cone. nag. IDav
51.87 92.97 216.31 325.31 750.60 1965.87 3676.73 5125.94 7909.42
2007.43 2569.44 2199.84 . 2149.83 2714.90 2299.14
2120.11 2651.97 1785.69 `2772.88
5.86 bb 12.91 MM 34.09 MM 52.81 fab 125.83 bb 334.50 bb 628.26 bb 877.10 bb 1355.04 bbX
341.64 MM 438.13 MM 374.67 MM 366.09 Mi 463.11 MM 391.72 bb
360.98 MM 4S2.31 MM 303.56 MM 473.07 MM
,
.*
-2 8
14 -12
5 12
5 -2 13
.. *V
*t
,
i j
31 4Ehki . \||r
/ 58
1979.57
336.85 bb
13
2184.76
372.08 bb-
24
2064.91
2303.09 998.60
1350.47 1319.16
233.79 175.09 202.65 1298.25
351.50 MM
392.40 bb 168.41 bb
jl 228.83 MM
223.45 bb
37.09 bb 27.01 MM 31.74 bb 219.86 bb
18
\
jtja
-25
( ni
>/J ]y
-26
46.35 98.73 177.97 . 328.19 730.44 1709.98 3402.77 5157.15 7163.23
4.91 MM 13.90 bb 27.51 Ml 53.30 MM 122.37 MM 290.56 MM 581.22 MM 882.46 MM 1226.91 hfaX
-18 16 -8
-11 2
-3 -3 -2
2
2 \r* aillI
Cal.
cjkjf *
3 Ivtrt ouHierj, w U c
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Ameli 062496t quantitated and printed on 03/17/99 fay ZAC
chetanti.
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Amalla 00491; quantitatad and prlatad on 03/17/99 fey LAC
Quantify Calibration ftaport 10X120 Hat Llvar Group 5 and 0
Calibration: a:\rata2.mo\CurwaDB\031C99fa2
Last Modified: ad Mar IT 13:00:30 1890
Printed:
Wad Mar 17 15:01:43 1999
] ] 1 T ]
J I ] !
!
Aaalia 062498: quantittad and printed on 03/17/89 fay U C
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Paga 1
1
' Quantify Calibration Raport 70X120 Rat Livar Group 8 and t
( Calibration i Os \rata2. VR0\CurvaDR\031C99b2
Laat aodiflod: Wad Mar 17 15:04:50 1999
1 Printad:
Wad Mar 17 15:01:48 1999
i l
J
I
J 1 J
]
]
:i
J
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1 Method Report
1
Method file: Last modified:
Conpound:
Printed:
G:\rats2.PRO\HethDB\031699b2 .mdb Wed Mar X7 06:39:09 1999
1: PPHS
Wed Mar 17 13:11:35 1999
Coapoond Nu m i Internal Ref.: Quantify Trace: Cone, of Standards:
User RP Value: User Peak Factor: Integrate Window Factor:
PPHS (Mone] 399.00 > 80.00 Cone. A
.
1.000000, Diaablad 1.000000
2.000
1
Retention Time (nine) : Relative Retention Time: Tine Window (mins) :
Peak Selectlonr
.
J Response Type: iHpaiHi
7.343 enabled 0.000, Diaablad 0.620
Nearest
Rxtemal Araaa . '
1
Polynomial Type: Point of Origin: Fit weighting: Axis Transformation:
T Units:
Peak-to-peak anplifcude:
Hau Exclude l/X None
293
Smoothing: Window size (scans): Number of smooths: smoothing method:
. Disabled 3 2
Join valley threshold: * Iteduc. peak tailing i Raise baseline maximum:
30.00 70.00 4.00
Draw vartical threahold:
10.00
Detect shoulders:
Diaablad
.
Shoulder threshold:
10.00
J Relative height: Absolute height:
50.00. Enabled 0.00 Disabled
Relative area: .
3.00, ftiablad
Absolute area:
0.00. Disabled
Afful/. ou 2 m
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>ag.
l A C O Ii/ m
As
Method Report
Method fil.i Last modified:
Compound:
Printed:
Q !\rat2.PRO\MthDB\031699b2 .db Mad M*r 17 06: S 09 1511
2: Surr
Mod Mir 17 13:11:35 1 5
Compound Mamet Internal Ref.: Quantify Trace: Cone, of Standards:
User RF Value: User Peak Factor: Integrate Window Factor:
Surr [Kona]
TIC
rixtd
'
1.000000, Disabled
1.000000
2 .0 0 0
Retention T i m (mins): Relative Retention Time: T i m Window (mins) :
Peak Selection:
1 Response Type: . Response:
Polynomial Type: Point of Origin: Fit Weighting: Axis Transformation:
8.231, Enabled 0.000, Disabled 0.588 Nearest
M aternal Areas
Exclude None None
| Units:
Feak-to-peak anplitude:
|
[ Smoothing: ` Window else (scans): Number of smooths: Smoothing method:
(i i Join valley threshold: *
Reduce peak tailing: Raise baseline maxixauu
77
Disabled 3 2
30.00 70.00 4.00
| Draw vertical threshold:
10.00
Detect shoulders:
Disabled
Shoulder threshold:
,* 10.00
| Relative height: 1 Absolute height:
Relative area: .
Absolute area:
50.00,, babied . 0.00. Disabled
3.00, babied
0.00, Disabled
* .
A iJ i& o u m t
___ l A t -
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Pag.
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FACT: DATAREVIEWSUMMARYFORM
Date: s / s h
Analyst: FACTStudy Number ~ 7 ^ k - / g > o
Matrix
1 TimePoints Dilutions?
3 Analyte 1:
\" j
/\J& Pofi
rb P f t J S ( S g ~
&>**
Acceptable LinearRange: 5 ?
LQfor this dataset:
^/>L
T Re-evaluatedfix differentrange?
Analyte 4:
Acceptable LinearRange: LOQfor this dataset: Re-evaluatedfor different range?
Anaiyte5: AcceptableLinearRange: LOQfor this data set Ro-evaluatedfbr differentrange?
Analyte 2:
Acceptable LinearRange: / / - ST r
LOQfor ds data set J /
Re-evaluatedfor differentrange? _______
J Analyte 3:.
'
Acceptable LinearRange: 5~ -
] LOQfarthis dataset Re-evaluated fbr different range?
Analyte 6: AcceptableLinearRange:
Z
LOQfor this dataset:
Re-evaluatedfor different,
Analyte 7:
7
Acceptable LindarRange:
LOQfix this dataset
Re-evaluatedfor different range?
J'
!
BACK TO MAIN
Quantify Compound Suaaary Rapert
Tox-120 Rat Liv.r
'
Saltila Listi H:\rats2.PR0\Saapla0B\04209So
Last modified: Thu Apr 22 09:02:11 1999
Mathed:
M:\rata2.PRO\MathDB\042099e
Last modifiad: Tua May 04 08:S2:4S 1999
Job Coda:
Printad:
Rad May OS 11:10:51 1999
Compound li POAA
# Hu m 1 042099002 2 042099003 3 042099004 4 042099005 5 042099006 6 042099007
7 042099008 8 042099009 9 042099010 10 042099011 11 042099012 12 042099013 13 042099014 14 042099015 15 04209901 1 042099017 17 04209901* 19 042099019 19 042099020 20 042099021 21 042099022 22 042099023 23 042099024 24 042099025 25 042099026 26 042099027 27 042099028 28 042099029 29 042099030 30 042099031 31 042099032 32 042099033 33 042099034 34 042099035 35 042099036 36 042099037 37 042099038 38 042099039 39 042099040 40 042099041 41 042099042 42 042099043 43 042099044 44 042099045 45 042099046 46 042099047 47 042099048 48 042099049 49 042099050 50 042099051 51 042099052 52 042099053 53 042099054 54 042099055 55 042099056
56 042099057 57 042099058 59 042099059 59 042099060 60 042099061 61 042099062 62 042099063 63 042099064 64 042099065 65 042099066 6 042099067 67 042099068 68 042099069 69 042099070 70 042099071 ,71 042099072 72 042099073 73 042099074
74 042099075 75 042099076 76 042099077 77 042099078 78 042099079 79 042099090 80 042099081 81 042099082
82 042099083 83 042099084 84 042099085
85 042099086 86 042099087 87 042099088 88 042099089
Samplallat Taxt MaOH Blank
TOX120, RBL0419-H2OBlk-l, 4/15/99
TOX120, RBL04199-LlvBlk-l, 4/19/99 TOX120, RBL04199-Sppb-1. 4/19/99
TOX120, RBL04199-10ppb~l, 4/19/99
TOX120, RBL04199-2Sppb-l, 4/19/99
Toxl20, RBL04199-50ppb-l, 4/19/99
TOX120, RBL04199-100ppb-l, 4/19/99
Toxl20, RBL04199-250ppb-l, 4/19/99
TOX120, RBL04199~500ppb~l, 4/19/99
TOX120, RBL04199-7S0ppb-l, 4/19/99
Toxl20, RBL04199-1000ppb-l, 4/19/99
MaOH Blank
TOX120, RTL, 1M, Cl, 4/19/99
TOX120, RTL, 2M, Cl, 4/19/99
TOX120, RTL, 3M, Cl, 4/19/99
TOX120, RTL, 4M, Cl, 4/19/99
TOX120, RTL, 5M, Cl, 4/19/99
TOX120, RBL04199-250ppb-CCl, 4/19/99
MaOH Blank
Toxl20, RTL, CM, Cl, 4/19/99
TOX120, RTL, 7M, Cl, 4/19/99
TOX120, RTL, 8M. Cl, 4/19/99 TOX120, RTL, 9M, Gl, 4/19/99
TOX120, RTL, 10M, Cl, 4/19/99
Toxl20, RBL04199-250ppb-CC2, 4/19/99
MaOH Blank
TOX120, RTL, 11M, Cl, 4/19/99
TOX120, RTL, U M , Cl, 4/19/99
. Toxl20, RTL, 14M, Cl, 4/19/99
Toxl20, RTL, ISM, Cl, 4/19/99
Toxl20, RBL04199-250ppb-CC3, 4/19/99
MaOH Blank
TOX120, RTL, 17M, G2, 4/19/99
TOX120, RTL, 18M, G2, 4/19/99
TOX120, RTL, 19M, G2, 4/19/99
TOX120, RTL, 20M, G2, 4/19/99
Toxl20, RTL, 21M, G2, 4/19/99
TOX120, RBL04199-250ppb-CC4, 4/19/99
MaOH Blank
Toxl20, RTL, 22M, G2, 4/19/99
TOX120, RTL, 23M, G2, 4/19/99
TOX120, RTL, 24M, G2, 4/19/99
Toxl20, RTL, 25M, G2, 4/19/99
Toxl20, RTL, 26M, G2, 4/19/99
Toxl20, RBL04199-250ppb-CC5, 4/19/99
MaOH Blank
TOX120, RTL, 27M, G2, 4/19/99
Toxl20, RTL, 29M, G2, 4/19/99
TX120, RTL, 30M, G2,
4/19/99'
TOX120, RTL, 31M, G2, 4/19/99
Toxl20, RBL04199-250ppb-CC6, 4/19/99
MaOH Blank Toxl20, RTL, 33M, G3, 4/19/99
TOX120/ RTL, 34M, G3, 4/19/99
- Toxl20, RTL, 3SM, Gl, 4/19/99 TOX120, RTL, 36M, G3, 4/19/99
TOX120, RTL, 37M, G3, 4/19/99
TOX120, RBL04199-250ppb-CC7, 4/19/99
MaOH Blank -
.
' TOX120, RTL, 38M, G3, 4/19/99
Toxl20, RTL, 39M, G3, 4/19/99
TOX120, RTL, 40M, G3, 4/19/99
' Toxl20, RTL, 41M, G3, 4/19/99
Toxl20', RTL, 42M, G3, 4/19/99
TOX120, RBL04199-250ppb-CC8, 4/19/99
MaOH Blank
Toxl20, RTL, 43M, G3, 4/19/99
Toxl20, RTL, 4SM, G3. 4/19/99
Toxl20, RTL, 46M, G3, 4/19/99
, Toxl20, RTL, 47M, G3, 4/19/99
TOX120, RBL04199-2S0ppb-CC9, 4/19/99 MaOH Blank
TOX120, RTL, 5M, G5, 4/19/99
TOX120, RTL, 66M, CS, 4/19/99
Toxl20, RTL, 73M, G5, 4/19/99 '
TOX120, RTL, 74M, C5, 4/19/99
TOX120, RTL. 75M. C5, 4/19/99
Toxl20, RBL04199-250ppb-CC4, 4/19/99
MaOH Blank
TOX120, RTL, 77M, C5, 4/19/99
Toxl20, RTL, 78M, GS, 4/19/99
Toxl20, RTL, 79M, CS, 4/19/99 ToxllO, RBL04199-250ppb-CCS, 4/19/99
MaOH Blank
Toxl20, RTC, 3M, Gl-2S0ppb-MS, 4/19/99
TOX120, RTL, 3M, Gl-2S0ppb-M3D, 4/19/99
TOX120, RTL, 6M, Gl-2S0ppb-MS, 4/19/99
Std Conn
Standard Standard Standard . Standard Standard Standard Standard Standard . Standard
5.56 11.10 27.80 55.60 111.00 278.00 556.00 833.00 1111.00
QC 278.00
QC 278.00 QC 278.00
QC ' 278.00
QC 278.00 QC 278.00 QC 278.00
QC 271.00 QC 278.00
QC 278.00 QC 278.00
RT
4.483
4.480 4.477 4.479 4.479 4.486 4.484 4.481 4.484
4.483
4.480
4.484
4.480 4.482 4.480 4.471 4.485 4.476
4.479 4.479 4.487 4.485 4.484 4.479
4.484 4.483 4.479 4.479 4.483 4.481 4.479 4.479 4.495 4.453 4.495
4.472 .4.463
4.475 4.488 4.481 4.480
4.483 4.479 4.480 4.486 4.484
4.454
4.459
4.477 4.472 4.485
9aga 1
Am
74
57 77 121 165 374 740 922 1075
Ratpon* Cono. Tlaga %Dv
73.837
57.350 76.531 121.098 165.361 374.157 739.533 921.621 1074.879
32.87 MM
20.70 MM 34.85 MM 67.75 MM 100.42 bb 254.53 bb 524.21 MM 658.60 bbX 771.72 bbX
86 25 22 -10 -8 -6 -21 -31
355 354.908 240.32 bb
447 447.316 308.53 bb
341 341.490 230.42 MM
3899 5143 5371 5320 5764
413
3898.674 5142.572 5371.353 5319.878 5764.340
413.054
2855.93 bb 3774.03 bb 3942.89 bb 3904.90 bb 4232.95 bb
283.24 bb
4423 3922 5424 2795 3068
343
4422.820 3922.056 5424.250 2794.636 3067.762
342.693
3242.79 bb 2873.19 bb 3981.94 bb 2041.05 bb 2242.64 bb
231.30 MM
2119 1448 1545 1644
419
2119.342 1448.030 1544.621 1644.361 418.663
1542.63 bb 1047.14 bb 1118.43 bb 1192.05 bb
287.38 bb
4232 5668 4583 4657 5081
411
4232.225 5668.U9 4582.970
4657.182 5061.245
411.447
3102.12 bb 4161.93 bb 3361.00 bb
3415.77 bb 3728.77 bb
202.05 MM
5631
5631.017 4134.55 m
4587
4517.391 3364.27 Mf
5604 . 5603.629 4114.33 bb
3410
3409.806 2495.10 bb
1319
1318.606 951.61 bb
443 442.641 305.07 bb
2595
1076 ISO
1384 398
2594.520 1075.927
850.322 1383.690
398.107
1893.35 bb 772.50 bb 605.98 bb 999.65 bb 272.20 MM
413 413.360 283.48 MM
364 363.646 246.77 MM 372 372.063 252.98 bb 380 380.105 258.92 MM 385 384.795 262.38 bb
.madalina 041099: :quantltatad and printad by maa 5/05/99
i
/
aadalina 04X099: squantitatad and printad by b m 5/05/99
L -- _i
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BACK TO MAIN
Quantify Compound S u n u r y Roport Tox-120 Rat Livor
Sampl. Lit: N:\rata2.PRO\SaplaOB\042099a
Laflt nodifiad: Thu Ape 23 09:02:11 1999
Mathod:
M:\zata2.HU3\MathOB\042099c
La.t nodifiad: Tua May 04 09:32:49 1999
Job Coda:
Prlntad:
Wad May 03 11:10:31 1999
Compound 2: PF0S
Mam*
Sanplelist Tuet
1 042099002
MeOH Blank
.
2 042099003
Toxl20, RBL04199-H20Blk-l, 4/19/99
3 042099004
Toxl20, RBL04199-LivBlk-l, 4/19/99
4 042099005
Toxl20, RBL04199-5ppb-l, 4/19/99
5 042099006
TOX120, RBL04199-10ppb-l, 4/19/99
6 042099007
TOX120, RBL04199-25ppb-l# 4/19/99
7 042099006
TOXI20, RBL04199-50ppb-l, 4/19/99
8 042099009
Toxl20, RSL04199-1OOppb-1# 4/19/99
9 042099010
TOX120, RBL04199-250ppb-l, 4/19/99
10 042099011
TOX120, RBL04199-5OOppb-1, 4/19/99
11 042099012
Toxl20, RBL04199-750ppb-l, 4/19/99
12 042099013
TOX120, RBLQ4199-1000ppb-l, 4/19/99
13 042099014
MeOH Blank
14 042099015
TOX120, RTL,'1H, Cl, 4/19/99
15 042099016
Toxl20, RTL, 2K, Gl, 4/19/99
16 042099017
Toxl20, RTL, 3M, Gl, 4/19/99
17 042099018
TOX120, RTL, 4M, Gl, 4/19/99
18 042099019
TOX120, RTL, SM, Gl, 4/19/99
19 042099020
TOX120, RBL04199-250ppb-CCl, 4/19/99
20 042099021
M*OH Blank
21 042099022
Toxl20, RTL, 6M, Gl, 4/19/99
22 042099023
Toxl20, RTL, 7M, Gl, 4/19/99
23 042099024
TOX120, RTL, 8M, Gl, 4/19/99
24 042099025
Toxl20, RTL, 9H, Gl, 4/19/99
25 042099026
Toxl20, .RTL, ICH, Gl, 4/19/99
26 042099027
Toxl20, RBL04199-250ppb-CC2, 4/19/99
27 042099028
MeOH Blank
28 042099029
Toxl20, RTL, 11M, Gl, 4/19/99
29 042099030
TOX120, RTL, 13M, Gl, 4/19/99
30 042099031 . Toxl20, RTL, 14M, Gl, 4/19/99
31 042099032
Toxl20, RTL, 15M, Gl, 4/19/99
32 042099033
TOX120, RBL04199-250ppb--CC3, 4/19/99
33 042099034
MeOH Blank
34 042099035
Toxl20, RTL, 17M, G2, 4/19/99
35 042099036
Toxi20, RTL, IBM, G2, 4/19/99
36 042099037
Toxl20, RTL, 19M, G2, 4/19/99
37 042099038
Toxl20, RTL, 20M, G2, 4/19/99
38 042099039
Toxl20, RTL, 21M, G2, 4/19/99
39 042099040
Toxl20, RBL04199-250ppb-CC4, 4/19/99
40 042099041
MeOH Blank
41 042099042
Toxl20, RTL. 22M, G2, 4/19/99
42 042099043
. TOX120, RTL, 23M, G2, 4/19/99
43 042099044
Toxi20, RTL. 24M, G2, 4/19/99
44 042099045
Toxl20, RTL. 25M, G2, 4/19/99
45 042099046
TOX120, RTL, 26M, G2, 4/19/99
46 042099047
Toxl20, RBL04199-250ppb-CC5, 4/19/99
47 042099048
MeOH Blank
48 042099049
Toxl20, RTL, 27M, G2, 4/19/99 *
49 042099050
TOX120, RTL, 29M, G2, 4/19/99
50 042099051
Toxl20, RTL, 30M, G2, 4/19/99
51 042099052 . Toxl20, RTL, 3IM, G2, 4/19/99
52 042099053
Toxl20, RBL04199-250ppb-CC6, 4/19/99
53 042099054
MeOH Blank
54 042099055 55 042099056
Toxl20, RTL, 33M, G3, 4/19/99 Toxl20,' RTL, 34M, S3, 4/19/99
56 042099057
Toxl20, RTL, 35M, G3, 4/19/99
57 042099058
Toxl20, RTL, 36M, G3, 4/19/99
58 042099059
Toxl20, RTL, 37M, G3, 4/19/99
59 042099060
Toxl20, RBL04199-2S0ppb-CC7, 4/19/99
60 042099061
MeOH Blank -
61 042099062
TOX120, RTL, 38M, G3, 4/19/99
62 042099063
Toxl20, RTL, 39M, G3, 4/19/99
63 042099064
TOX120, RTL, 4QM.G3, 4/19/99
.64 042099065
TOX120, RTL, 41M, G3, 4/19/99
65 042099066
Toxl20; RTL, 42M, G3, 4/19/99
66 042099067
TOX120, RBL04199-250ppb-CC8, 4/19/99
67 042099068
MeOH Blank
68 042099069
Toxl20, RTL, 43M, G3, 4/19/99
69 042099070
Toxl20, RTL, 45M, G3, 4/19/99
70 042099071 71 042099072
Toxl20, RTL, 46M, G3, 4/19/99 T0XI20, RTL, 47M, 63, 4/19/99
72 042099073
' Toxl20, RBL04199-250ppb-CC9, 4/19/99
73 042099074
MeOH Blank
74 042099075
TOX120, RTL, 65M, GS, 4/19/99
75 042099076
Toxl20, RTL, 66M, GS, 4/19/99
76 042099077
TOX120, RTL, 73M, GS, 4/19/99
77 042099078
TOX120, RTL, 74M, G5, 4/19/99
78 042099079
Toxl20, RTL. 75M, G3, 4/19/99
79 042099080
Toxl20, RBL04199-250ppb-CC4, 4/19/99
80 042099081
MeOH Blank
.
81 042099082
Toxi20, RTL, 77H, G5, 4/19/99
82 042099083
Toxl20, RTL, 78M, 65, 4/19/99
83 042099084
OX120, RTL, 79M, G5, 4/19/99
84 042099085
TOX120, RBL04199-250ppb-CC5, 4/19/99
85 042099086 * MeOH Blank
86 042099087
Toxi20, RTL, 3M, Gi-250ppb-MS, 4/19/99
87 042099088
Toxi20, RTL, 3M, Gl-250ppb-KS0, 4/19/99
88 042099089
Toxl20, RTL, 6M, Gl-250ppb-MS, 4/19/99
typa
Std Cono
Standard Standard Standard Standard Standard Standard Standard Standard Standard
5.56 11.10 27.80 55.60 111.00 278.00 556.00 833.00 1111.00
QC 278.00
OC 278.00 QC 278.00
QC 278.00
QC 278.00 QC 278.00 QC 278.00
QC 278.00 QC 278.00
QC 278.00 QC 278.00
RT
4.822 4.823 4.819 4.816 4.819 4.818 4.825 4.823 4.820 4.779
4.822 4.818 4.824 4.817 4.780 4.822
4.824 4.82t) 4.823 4.823 4.821 4.820
4.823 4.824 4.824 4.821 4.824
4.819 4.821 4.819 4.810 4.779 4.813
4.819 4.819 4.827
4.824 4.778 4.818
4.823 4.822 4.818 4.819 4.823
4.820 4,819 4,818 4.787
4.789 4.788
4,811 4.803 4.813 4.782 4.820 4,819
4.823 4.818 4.820 4.825 4.823
4.824 4.829 4,780 4.790 4.804 4,791
.
4.810 4.816 4.773 4.795 4.792 4.816 4.811 4.781
Area
Raapon. Cono. Flage tDav
114 142 182 310 510 835 1972 3926 5290 * 6382
113.879 142.359 181.B4S 310.291 510.134 834.975 1972.331 3925.785 5290.446 6342.225
0.00 bb 3.38 bb 8.76 bb 26.24 bb 53.43 bb 97.63 bb 252.40 bb 518.22 MM . 703.92 MMX 852.48 bbX
-39
-2-16
-4
-12 -9
-7 -IS
-23
1409 1067
863 1032
874 2082
1409.125 1086.545 ' 863.333 1032.293
674.073 2081.914
175.7 bb 129.14 bb 101.49 bb 124.48 bb 102.95 MM 267.31 bb
1007
1047 1342
956 2596 2257
1007.439 1046.567 1341.526 956.335 2595.882 2256.951
121.10 bb 126.43 bb 166.56 bb 114.15 bb 337.25 bb 291.13 bb
`2384 804 907 813
1926
2363.945 604.193 906.706 813.172
1926.462
308.41 bb 93.44 bb
107.39 bb 94.67 bb
246.16 bb
514 413 411 383 371
2309
514.195 412.578 411.485 343.146 370.594 2309.480
53.98 bb 40.16 bb 40.01 bb 36.15 bb 34.44 bb 298.28 bb
309 259 330 360 307 2006
309.189 259.486 330.015
360.292 307.436 2006.424
26.09 bb 19.32 bb 28.92 bb 33.04 bb 25.85 bb 257.04 bb
304 444 810 349 2312
304.184 444.249 810.409 346.586 2312.079
25.41 bb 44.46 bb 94.29 bb 31.45 bb ' . 298.63 bb
642 . 642.037 . 71.38 bb 4B1 484.537 50.22 bb
587 596.903 63.88 bb
545 544.943 58.17 bb 669 669.401 75.10 bb
2265
2264.934 292.22 bb
472 4
472.107
48.26 bb
713* x 713.120 61.05 bb
60S 604.755 93.52 bb
421 421.200 *41.33 bb 588 587.601 63.97 bb
2329
2328.665 300.89 bb
487
559 509 530 2293
466.624 558.781 509.226 529.702 2293.406
50.23 bb 60.05 bb 53.31 bb 56.09 bb 296.09 bb
.
328 660 1251 825 781 2331
326.225 660.033 1251.132 125.343 780.944 2330.889
28.68 MM 73.83 bb 154.26 bb 96.32 bb 90.28 bb 301.19 bb
974 767 730 2166
50 2869 2955 3061
973.805 767.281 729.592 2166.195
50.002 2669.270 2954.571 3060.699
116.52 bb 88.42 bb
83.29 bb 278.78 bb 0.00 bb 374.45 bb 386.06 bb 400.50 bb
Pag* 3
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BACK TO MAIN
1 ________ :______ -
Quantify Conpound Suanary Raport Tox-120 Rat Llvar
-
I Saopla Lit: R:\rata2.PR0\SaaplaOB\04209Ra . Lait Bsdlfiad! Thu Apr 22 09:02:11 1999
Mathod:
M:\rata2.PR0\MathOB\042099o
Laat aodlflad: Tua Hay 04 08:S2:4B 1999
Job Coda:
'
] Brintad:
ad May OS 11:10:51 1999
;
] ]
Conpound 3: FTHS
i Rana 89 042099090 90 042099091 91 042099092 92 042099093 93 042099094 94 042099095 95 042099096 9$ 042099097 97 042099098 98 042099099 99 042099100 100 042099101 101 042099102 102 042099103 103 042099104 104 042099105
Sanplali.t Taat
*
TOX120, RTL, H, 61-2S0ppb-M3D, 4/19/99
8td Cono
TOX120, RTL. 13M, Gl-2S0ppb-MS, 4/19/99
TOX120, RTL, 13M, Gl-250ppb-MSD, 4/19/99
TOX120, RBL04199-250ppb-CC6, 4/19/99
QC
278.00
MaOH Blank
TOX120, RBL04199-H2OBlk-l. 4/19/99
TOX120, RBZ.04199-LivBlk-l, 4/19/99
.
Toxl20, RBL04199-Sppb-1, 4/19/99
Standard
5.56
Toxl20, RBL04199-10ppb-l, 4/19/99 - Standard
12.10
Toxl20, RSL04199-2Sppb-l, 4/19/99
Standard
27.80
Toxl20, RBL04199-50ppb-l, .4/19/99
Standard , 55.60
TOX120, RBL04199-100ppb-l, 4/19/99
Standard
111.00
TOX120, RBL04199-2S0ppb-l, 4/19/99
Standard
278.00
TOX120, RSL04199-S00ppb-1> 4/19/99
Standard
556.00
TOX120, RBL04199-7S0ppb-l, 4/19/99
Standard
833.00
TOX120, RBL04199-1000ppb-i;- 4/19/99
Standard 1111.00.
. RT 4.115 4.117 4.117 4.109 4.117 4.161 4.162 4.112 4.117
4.116 4.116 4.119 4.103 4.117 4.116 4.119
BACK TO MAIN '
, ___
Paga ( -
..
. '
At m 6240 7204 7649 5035
53 49 43 126 237 597 1283 2466 5941 11407 16687
18582
Raapons 6240.264 7203.939 7648.546 5035.472
52.570 49.377 42.961 126.050 . 237.146 596.977
1282.536 2465.863 5941.001 11406.754 16686.936 18581.658
Cono. Flags %Dv
.
321.94 bb 371.71 bb 394.66 bb
\ ) j C a hrul
259.74 MM
-7
2.45 MM
2.29 MM
1.96 MM
6.25 MM
12
11.98 bb
8
30.56 bb
10
65.96 bb
19
127.06 MM
14
306.50 bb
10
588.71 bb
6
861.35 bb
3
959.18 bbX -14 *
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I mad*lina 041099: :quanti tt*d arid print* by mmm 5/05/99
BACK TO MAIN
1
--
-
i --------------------- - _____________ ___________________________________ r:---------------------------------------------
Quantify Compound Sumnary fUport Tox-120 Rat Livor
~ * Pag* ?
Sampla Lift: H:\nts2.PftO\StmplsOB\042099c
Last modified: Thu Apr 22 09:02:11 1999
Mothod:
N:\rats2.PRO\MathDB\042099a
Last modiflad: Tua May 04 08:52:48 1999
Job Coda:
Printad:
Wad May 05 11:10:51 1999
1 J 1
I
j
J
3
!
Compound 4: ZS
Mama 1 042099002 2 042099003 3 042099004 4 042099005 5 042099006 042099007 7 042099008 8 042099009 9 042099010 10 042099011 11 042099012 12 042099013 13 042099014 14 042099015 15 042099016 16 042099017 17 042099018 18 042099019 19 042099020 20 042099021
21 042099022 22 042099023 23 042099024 24 042099025 25 042099026 26 042099027 27 042099028 28 042099029
29 042099030 30 042099031 31 042099032 32 042099033 33 042099034 34 042099035 35 042099036 36 042099037 37 042099038 38 042099039 39 042099040 40 042099041 41 042099042 42 042099043 43 042099044 44 042099045 45 042099046 46 042099047 47 042099048 48 042099049 49 042099050 50 042099051 51 042099052 52 042099053 53 042099054 54 042099055 55 0 4 2 0 9 9 0 5 6 56 042099057. 57 04209905B 58 042099059
59 042099060 60 042099061 61 042099062 62 042099063 63 042099064 64 042099065 65 042099066 66 042099067 67 042099068 68 042099069 69 042099070 70 042099071 71 042099072
72 042099073 73 042099074 74 042099075 75 042099076 76 042099077 77 042099078 78 042099079
79 042099080 80 042099081 81 042099082
82 042099083 83 042099084 84 042099085
85 042099086 86 042099087 87 042099088
88 042099089
Sainplaliat Text
M.0H Blank
TOX120, RBL04199-H20BU-1, 4/19/99
IOX120, RBL04199-LivBlk-l, 4/19/99
Toxl20. R8L04199>5ppb-l, 4/19/99
Toxl20, RBL04199-10ppb-l, 4/19/99
TOX120, BBL04199-2Sppb-l. 4/19/99
Toxl20. RBL04199-S0ppb-1, 4/19/99
TOX120. RBL04199-100ppb-l, 4/19/99
TOX120, RBLO4199-2S0ppb-l, 4/19/99
TOX120, RBL04199-500ppb-l, 4/19/99
Toxl20. RBL04199-7S0ppb-l, 4/19/99
TOX120, RBL04199-1000ppb-l, 4/19/99
HaOH Blank
TOX120, RTL, IN, Gl, 4/19/99
TOX120, RTL, 2M, Gl, 4/19/99
TOX120, RTL, 3M, Gl, 4/19/99
TOX120, RTL. 4M, Gl, 4/19/99
TOX120, RTL, SN, Gl, 4/19/99
.
TOX120, RBL04199-250ppb-CCl, 4/19/99
HaOH Blank
TOX120, RTL, CM, Gl. 4/19/99
TOX120. RTL, 7H, Gl, 4/19/99
Toxl20, RTL, 8M, Gl, 4/19/99
TOX120, RTL. 9M, Gl, 4/19/99
TOX120, RTL, 10H, Gl, 4/19/99
TOX120, RBLO4199-2S0ppb-CC2, 4/19/99
HaOH Blank
TOX120, RTL, 11M, Gl, 4/19/99
TOX120, RTL, 13M, Gl, 4/19/99
TOX120, RTL, 14H, Gl, 4/19/99
.
* TOX120, RTL, ISM, Gl, 4/19/99
TOX120, RBL04199-250ppb-CC3, 4/19/99
HaOH Blank
TOX120, RTL, 17N, G2, 4/19/99
TOX120, RTL, ISM, G2, 4/19/99
TOX120, RTL, 19M, G2, 4/19/99
TOX120, RTL, 20H, G2, 4/19/99
TOX120, XTL, 21M, G2, 4/19/99
TOX120, RBL04199-250ppb-CC4, 4/19/99
MeOH Blank
Toxl20, RTL, 22M, G2, 4/19/B9
Toxl20, RTL, 23M, G2, 4/19/99
' TOX120, RTL. 24M, G2, 4/19/99
TOX120, RTL, 25M, G2, 4/19/99
.
Toxl20, RTL, 26M, G2, 4/19/99
TOX120, RBL04199-2S0ppb-CCS, 4/19/99
HaOH Blank
Toxl20, RTL, 27M, G2, 4/19/99'
TOX120, RTL, 29M, G2, 4/19/99
TOX120, RTL, 30M, G2, 4/19/99
TOX120, RTL, 3IN, G2, 4/19/99
TOX120, RBL04199-2S0ppb-CCC, 4/19/99
HaOH Blank
TOX120, RTL, 33H..G3, 4/19/B9 TOX120, RTL. 34M, G3, 4/19/99
Toxl20, RTL. 35M, G3, 4/19/99
TOX120, RTL, 3M, G3, 4/19/99
TOX120, RTL, STM, G3, 4/19/99
TOXI20, RBL04199-250ppb-CC7, 4/19/99
HaOH Blank .
.
TOX120, RTL, 38H, G3, 4/19/99
TOX120, RTL, 39M, G3, 4/19/99
. TOX120, RTL, 4OH, G3, 4/19/99
TOX120, RTL, 4IN, G3, 4/19/99
Toxl20,.RTL, 42M, 63, 4/19/99 '
TOX120, RBL04199~230ppb-CCI, 4/19/99
HaOH Blank
TOX120, RTL, 43H, G3, 4/19/99
TOX120, RTL, 4SM, G3, 4/19/99
TOX120, RTL, 4CM, G3, 4/19/99
TOX120, RTL. 4TM, G3, 4/19/99
TOX120, RBL04199-250ppb-CC9, 4/19/99
HaOH Blank
Toxl20, RTL,. C5M, GS, 4/19/99
Toxl20, RTL, 6CM, GS, 4/19/99
TOX120, RTL, 73M, GS, 4/19/99
TOX120, RTL, 74M. G5, 4/19/99
TOX120, RTL, 75M, GS, 4/19/99
TOX120, RBL04199'2SOppb-CC4, 4/19/99
HaOH Blank
TOX120, RTL, 77M, GS, 4/19/99
TOX120, RTL, 78M, G5, 4/19/99
TOX120, RTL, 79M, G5, 4/19/99
TOX120, RBL04199'2S0ppb-CCS, 4/19/99
HaOH Blank
TOX120, RTJ|, 3M, Gl-2S0ppb-MS, 4/19/99
TOX120, RTL, 3H, Gl-25Oppb-MS0, 4/19/99
TOX120, RTL, 6M. Gl-250ppb-MS, 4/19/99
*n
Std Cono
Standard Standard Standard Standard Standard Standard Standard Standard , Standard
1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00
OC '
1.00
OC 1.00 .
oc 1.00
oc - 1.00 oc 1.00 oc 1.00 oc 1.00 oc 1.00 oc 1.00
oc 1.00
.
oc 1.00
M
ATM
Mssponsm Cono, Flsgs %0v
4.479 4.477 4.477 4.475 4.472 4.474 4.473 4.481 4.478 4.475 4.479
11168 14262 17108 14220 14163 15047 14165 12687 13127 12184 14980
11168.010 14262.124 17107.816 14220.119 14162.521 15047.055 14164.800 12686.997 13127.305 12183.721 14960.457
0.72 bb 0.92 bb 1.10 bb 0.92 bb 0.91 bb 0.97 bb 0.91 bb 0.82 bb 0.85 bb 0.79 bb 0.97 MK
-28 -8 10 -8 -9 -3 -9
-18 -15 -21
-3
4.477 4.473 4.479 4.473 4.480 4.477
13354 15480 16743 16108 16428 12965
13353.618 15479.964 16743.496 16107.631 16427.918 12964.758
0.86 bb 1.00 bb
1.08 bb 1.04 bb 1.06 bb 0.84 bb
-14 0 8 4 6
-16
4.479 4.476 4.478 4.478 4.476 4.475
12592 12908 14767
13113 . 16767
15329
12591.617 12908.252 14767.411 13113.133 16766.613 15328.990
0.81 bb 0.83 bb 0.95 bb . 0.85 bb
1.08 bb 0.99 bb
-19 -17
-5 -15
8 -1
4.476 4.479 4.479 4.476 4.479
.14482 11815 13252 12072 12820
14482.320 11815.149 13252.295 12071.706 12819.677
0.93 bb 0.76 bb 0.86 bb 0.78 bb 0.83 bb
-7
-24 -14 -22 -17
4.474 4.476 4.475 4.465 4.434 4.471
7188 6839 6975 7305 7576 15008
7188.418 6839.444
6975.442 7304.985 7576.205 15007.997
0.46 bb 0.44 bb 0.45 bb 0.47 bb 0.49 bb 0.97 bb
-54 -56 -55
-53 -51
-3
4.473
4.474 4.438 4.434 4.434 4.473
7420 8272 8265 9236 9268 14716
7419.602 8272.232 8264.774 9235.608 9268.021 14716.280
0.48 bb 0.53 bb 0.53 bb 0.60 MM 0.60 bb 0.95 bb
-52 -47 -47 -40 -40
-5
4.478 4.434 4.473 4.429 4.478
8715 12268 10776 11197 14400
8714.930 12268.174 10776.486 11196.844 14399.545
0.56 bb 0.79 bb 0.70 bb. 0.72 bb . 0.93 bb
-44 -21 -30 -28
-7
4.475 4.474 4.430
4.445 4.448
4.445
16537 8276 7717
9574 7747 15240
16537.041 274.272 7716.823
9574.318 7747.233
15240.495
1.07 bb 0.53 bb 0.50 bb
0.62 bb 0.50 bb
0.98 bb
7 -47 -50
-38 -50
-2
4.467
4.456 4.426 4.437 4.475 4.475
7589
7588.534
9947* 9947.499
7538
7537.861
8481 ' 8480.613
12183 12182.729
,14952 14951.948
0.49 bb 0.64 bb 0.49 bb * 0.55 bb 0.79 bb 0.97 bb
-51 -36 -51 -45 -21
-3
4.434 4.474 4.475 4.435 4.478
8922 9854 13112 9645 15910
8921.938 9854.359 13111.587 . 9645.204 15909.816
0.58 MM 0.64 bb 0.85 bb 0.62 bb 1.03 bb
-42 -36 -15 -38
3
4.478 ^' 10254
4.475
11430
4.434
14854
4.442
14617
4.461
14512
4.449
15752
10253.570 11430.374 14853.702 14617.353 14511.814 15752.205
0.66 bb 0.74 bb 0.96 MM 0.94 bb 0.94 bb 1.02 bb
-34 -2
-4 -6 . -6
2
4.465 4.473 4.428 4.453
26825 16234 16282 15674
26824.668 16234.444 16281.634 15674.282
1.73 bb 1.05 MM
1.05 MM 1.01 bb
73 5 5 1
4.472 4.466 4.480
15718 17154 1S585
15717.900 17154.322 15585.048
1.01 bb 1.11 bb 1.01 bb
1 11
1
madalina 041099::quantitatad and printad by maa 5/05/99
Quantify Compound Summary Raport Tox-120 Rat Livar
Sampla Liat: H:\rata2.PRO\3mqplmOB\042099a Last modifiedr Thu Apr 22 09:02:11 1999
Mathod:
K:\rata2.PRO\MathOB\042099e
Last modiflad: Tua Hay 04 0:S2:48 1999
Job Coda:
Printad:
Wad Hay OS 11:10:S1 1999
Compound 4: XS
Mama 89 042099090 90 042099091 91 042099092 92 042099093 93 042099094 94 04209909S 95 042099096 96 042099097 97 042099098 98 042099099 99 042099100 100 042099101 101 042099102 102 042099103 103 042099104 104 042099105
Saxplaliat fast
Typ
Std-Cono
TOX120, RTL, CM, Gl-250ppb-H3D, 4/19/99
Toxl20, RTL, 13M, Gl-250ppb-MS, 4/19/99
TOX120, RTL, 13M, Gl-2S0ppb-MSD, 4/19/99
10x120, RBL04199-250ppb-CC4, 4/19/99
oc
1.00
MeOH Blink
TOX120, RBL04199-H2OBlk>l, 4/19/99
TOX120, RBL04199-Llv81k-1, 4/19/99 TOX120, RBL04199-Sppb-1, 4/19/99 TOX120, RBL04199-10ppb-l, 4/19/99 . Toxl20, RBL04199-2Sppb-l, 4/19/99 TOX120, RBL04199-S0ppb-1. 4/19/99 Toxl20, RBL04199-100ppb-l, 4/19/99 TOX120, RBL04199-2S0ppb-l, 4/19/99 TOX120, RBL04X99-S00ppb>l, 4/19/99 TOX120, RBL04199-7S0ppb~l, 4/19/99 TOX120, RBL04199-I000ppl>-1,- 4/19/99
Standard Standard Standard Standard , Standard Standard
Standard Standard Standard
1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00
RT 4.477 4.478 4.435 4.470
4.478 4.478 4.473 4.435 4.433 4.434 4.433 4.465 4.434 4.434 4.435
Paga B
Araa 15039 14583 15562 16368
13605 17110 20728 17142 16514 17079 17088 15845 15402 15262 16108
Rasponsa 15038.934 14582.552 15562.266 16367.936
13604.745 17109.813 20728.434 17141.998 16513.951 17078.783 17087.787 15844.741 15402.126 15261.598 16108.356
Cone, flag %Dav
0.97 bb
-3
0.94 bb
-6
1.00 bb
0
1.06 bb
6
0.88 bb 1.10 bb 1.34 bb 1.11 bb 1.07 bb 1.10 bb 1.10 bb 1.02 bb 0.99 bb 0.99 MM 1 *04 bb
-12 10 34 11 7 10 10
2
-1 4
: .
madalina 041099: :quanti tatad and printad by maa S/OS/99
Quantify Calibration flaport Tox-120 Rat Livar
Calibration: N:\rata2.FRO\CurvaOB\042099o
Last nodifiad: Rad May 05 09:00:19 1999
Frintad:
Wad May 05 11:11:10 1999
'Compound 1 name: POAA : Coefficient of Determination: 0.989036
BACK TO MAIN
Paga 1
mdalin* 04 1099m quantit tad and prlntad by m-- 5/05/99
1 ________ Quantify Calibration Raport To*-120 Rat Livar
I Calibration: M:\rats2.PRO\CurvaDB\042099o
Last Modifiad: Had Hay OS 09:00:19 1999
Printad:
Wad Hay 05 11:11:10 1999
j
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J 1
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BACK TO MAIN
. Quantify Calibration Raport Tor-120 ]Ut U n c
"*
.
` p9 3
Calibration: N:\rati2.FWO\CurvaDB\042099c
Laat aodifiad: Wad May 03 09:00:19 1999
Frintad:
Wad May 03 11:11:10 1999
Quantify Calibration lUport Tox-120 Sat 1*1var
Calibration: H:\rati2.mo\CurvaD8\042099a
baat nodifiad: Wad Ky 05 09:00:19 1999
Printad:
Wad May 05 11:11:10 1999
-
* '
BACK TO MAIN
W9a 4 -
adallna 041099 :quantittad and. printad by aaa 5/05/99
Method Report
Method file: Last modified:
Compound: : *
Printed:
N:\rats2. FRO\MthOB\042099c.adb Tut Hay 04 08:02:41 1999C
1: P O M
Mad May S 11:10:26 1999
Compound Name:
Internal R.f.i Quantify Trace: Cone, of Standards:
User RF Value: User Peak Factor: Integrate Window Factor:
Peak Location
Retention Time (mins): Relative Retention Tine: Time Window (mins):
Peak Selection:
Response
Response Type: Response:
Calibration Curves
Polynomial Type: Point of Origin: Fit Weighting: Axis Transformation:
Concentration
Units:
Noise
Peak-to-peak ampintude:
Smooth
Smoothing: Window size (scans): Number of smooths: Smoothing method:
Baselines
Join valley threshold: Reduce peak tailing: Raise baseline maximum:
Peak Separation
Draw vertical thraahold:
Detect shoulders:
Shoulder threshold:
: %
Response Threshold
Relative height: Absolute height: Relative area:
Abaolutt araa:
POAA {None] TIC Cone. A 1.000000, Disabled
1.000000
2 .0 0 0
4.400, Enabled 0.000, Disabled 0.B34 Nearest
External Areas
Linear Exclude
1/X
None
434
Disabled '3
2
20.00 50.00 5.00
10.00 Disabled 40.00
1.50, Disabled 0.00, Disabled 2.00, Disabled 0.00, Dlsabltd
.
' .
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Pag. l
H ouU k u omirtE f
IAC, O Id I9
*
:
H.thod Report
Method file: Last modified:
Compound:
Printed:
N:\rats2.PRO\HethDB\042099c.mdb Tue Hay 04 04:52:4 19990
2: PFOS
Ned Hay 05 11:10:26 1999
Compound Name: Internal Ref.: Quantify Trac: Cone, of Standards:
pros
[None] TIC
Cone. A
.
*
Usar Rr Valu: Usar Peak Tactor: Intagrat Window Tactor:
1.000000, Disabled
1.000000
2.000
Paak Location
] Retention Tina Inina): Relative Retention Tine: Tina Window Inins):
Peak Selection:
4.780, Enabled 0.000, Disabled 0.834
Nearest
Response
] Response Type: Response: Calibration Curves
External Areas .
1
* Polynomial Type: Point of Origin: Tit Weighting: Axis Transformation:
Linear Exclude i/X None
1
Concentration Units: Noise Peak-to-peak amplitude: Smooth
434
Smoothing: Window site (scans): Number of smooths: Smoothing method:
Disabled 3 2
Baselines
Join valley threshold:
20.00
Reduce peak tailing:
50.00
Raiaa baaalina maximum: - , 3.00
Peak Separation
Draw vertical thraahold:
Detect ahould.rx;
Shoulder thraahold:
t
10.00 Disabled 40.00
J Response Threshold Relative height: Absolute height: Relative area:
Absolute area:
1.50, Disabled
0.00, Disabled * 2.00, Disabled
0.00, Disabled
]
I
i
J
!
-
" BACK TO MAIN
- .. P9. 2
M a W CSLcM
I C nL
Mathod Report
Method file: Last modified:
Compound:
Printad:
N:\rats2!.PRO\MethDB\042099c.ndb Tue May 04 08:52:48 19990
3: PFKS
Wed May 05 11:10:2 1999
'
Compound Name: Internal Ref.! Quantify Trace: Cone', of Standards:
User RF Value: User Peak Factor: Integrate Window Factor:
Peak Location
Rcttntion Time (mins): Relative Retention Time: Time Window (mins):
Peak Selection: Response Response Type: Response:
.
Calibration Curves
Polynomial Typo: Point of Origin: Fit Weighting:
Axis Transformation:
Concentration Units:
Noise
Peak-to-peak amplitude:
Smooth
Smoothing: Window size (scans):
Number of smooths: Smoothing method:
.
Baselines
Join valley threshold: Reduce peak tailing: Raise baseline maximum:
Peak Separation
Draw vertical threshold:.
Detect shoulders:
Shoulder threshold:
t
Response Threshold
Relative height: Absolute height: Relative area: Absolute areal
PFH5 (NoneJ
TIC Cone. A
'
1.000000, Disabled 1.000000
2.000
.
4.120 Enabled 0.000 Disabled 0.834
Nearest
.
External Areas
Linear
Exclude 1/X None
434
Disabled 3 2
20.00 50.00 5.00
10.00 Disabled 40.00
1.50, Disabled 0.00, Disabled 2.00, Disabled 0.00, Disabled
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Pege 2
M a c U i m , HlDtf LAC/ r /w f/tt
Method Report
Method file: Last modified:
Compound:
Frinttd:
N:\rata2.PRO\MathDB\042099c.Bdb Tu M a y 04 04:52:48 19990 4: IS Wed M ay 05 11:10:2 1999
Compound Nam*:
Internal Ref.: Quantify Trace: Cond. of Standard:
, IS
(None] TIC Fixed
User RF Value: User Peak Factor: Integrate Window Factor:
Peak Location
1.000000, Disabled
1.000000
2 .0 0 0
Retention Time (mins): Relative Retention Time: Time Window (mins):
Peak Selection:
Response
4.490, Enabled 0.000, Disabled 0.034
Nearest
Response Type: Response:
Calibration Curves
.
External Areas .
Polynomial Type: Point of origin: Fit Weighting: Axis Transformation:
Concentration
Linear Exclude 1/X None
Units:
Noise
Peak-to-peak amplitude: Smooth
434
Smoothing: Window size (scans):
Number of smooths: Smoothing method:
Disabled 3 2
Baselines
Join valley threshold: Reduce peak tailing: Raise baseline maximum;
Peak Separation
20.00
50.00
5.00
Draw vertical threshold:
Detect shoulders:
Shoulder threshold:
,
Response Threshold
10.00
Disabled 40.00
Relative height: Absolute height: Relative area: Absolute area:
1.50, Disabled 0.00, Disabled J 2.00, Disabled 0.00, Diaabled
.
BACK TO MAIN
. Fag 4
(WIDM ___HcuUiki
I
.. 1 AC. QSltX1m
ii
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AMELIA062498: FACT Equipment Log 99016 QuattroII,#6448E
PAGE
DATE: 04/20/99
.
ANALYST: mee
STUDY NUMBER Tox-120 MATTOX: Rat Liver DESCRIPTION: ip
FACT Method: ETS-8-5.0
Injectionvolume,10uL
Cycletime: 9.0min. Electrode:Z-Spray
MobilePhase:
(A )2.0m M NH4CH3CQ2
(B)MeOH
TN-A- orstndrdnumber (A) 99012-5-22
IB)TN-A-2414
Prepared(date/by): (A)4/16/99/ok
(B)4/9/99_hoj_
Expires:
(A) 10/99
(B) 10/99
Storeatroomtemp? [Y] or N
Other__
Guard column: None ___________
Partnumber na
Lot number na
Analyticalcolumn:keystonebetasil________________________
Size:2x 50mm____________ _ Type:_cl8________ Spheresize:5 um
Partnumber 055-701-2
Lotnumber P7L04 Serialnumber.0481172z
Analyticalparameters:
i
Dryinggas(L/hr):300
. ESInebulizinggas(L/hr)15
LC Flowrate:300uL/min. Backpressure:59bar
Sheathgas(L/hr)_0 ^
HPLC solventgradient:long2
Time (min.) 1) 0.00______
2) 4.5_______
3 6.5______ 5) 7.0______
%B 40
95
95 40_
:
'
-
'
Tuning Method Report
"
.. *
"
Method:
C :\MASSLYNX\RATS2. PRO\ACQUDB\PFOS
Printed:
Tue Apr 20 17:06:38 1999
1
" 11
7 / ^ - BACK TO MAIN
/^tT
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'.
PagQ 1
SOURCE ( ESP- )
Capillary Cone Extractor _ RF Lens Source Block Temp. Desolvation Temp.
Set Rdbk
3.00 15 5 0.2 150 250
2.99 -14 -6
152 258
Pressures
.
Analyser Vacuum tas Cell
Rdbk
1.6e-4 4.7e-3
MSI
LM Resolution
HM Resolution
Ion Energy
Ion Energy Ramp
Lens 6
.
Multiplier
Set Rdbk
- 15.0 15.0 2.0$ 0.0 57 650
MS2
LM Resolution
HM Resolution
Collision
.
Ion Energy
Ion Energy Ramp
Lens 8
Lens 9
Multiplier
Set Rd
15.0 15.0 30 2.0 0.0 40 0 650
32
39 30
FACT:DATAREVIEWSUMMARYFORM
BACK TO MAIN
Date:
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Quantify C alibration Aaport
C a lib ra tio n : C:\KMSUnoc\rata2.mo\CurvaDB\04209M L aat m o dlflad: Thu Apr 22 07:30:53 1 9 * 9
F rin ta d :
Thu Apr 22 07:31:SS 1*99
BACK TO MAIN
Amalia 092499: Raviawad and quantittad by kjh 4/22/99
Masslynx - Sample List
Sample List: C:\M ASSLYNX\rats2.PRO\Sam pleDB\042099c.SPL Printed: Tue Apr 20 16:51:50 1999
-
i/ B A C K T O M A IN
/r~ctr
, Pagel '.
File Nam e
File Text
1 042099002
MeOH Blank
2 042099003
Tox120, R B L04199-H 20B lk-1,4/19/99
3 042099004
Tox120, R B L04199-LivB lk-1,4 /19/99
4 042099005
Tox120, RBL04199-5ppb-1,4 /1 9 /9 9
5 042099006
Tox120, R B L04199-10ppb-1.4 /19/99
6 042099007
Tox120, RBL04199-25ppb-1,4 /1 9 /9 9
7 042099008
Tox120, RBL04199-50ppb-1,4 /1 9 /9 9
8 042099009
Tox120, RBL04199-100ppb-1, 4/19/99
9 042099010
Tox120, RBL04199-250ppb-1,4 /1 9 /9 9
10 042099011
Tox120, RBL04199-500ppb-1,4 /1 9 /9 9
11 042099012
Tox120, RBL04199-750ppb-1,4 /1 9 /9 9
12 042099013
Tox120, RBL04199-1000ppb-1,4 /1 9 /9 9
13 042099014
MeOH Blank
14 042099015
Tox120, RTL, 1M, G 1 .4/19/99
15 042099016
TOX120, RTL, 2M , G 1.4 /1 9 /9 9
16 042099017
Tox120, RTL, 3M, G 1 ,4/19/99
17 042099018
Tox120, RTL, 4M . G 1 ,4/19/99
18 042099019
Tox120, RTL, 5M, G 1 ,4/19/99
19 042099020
Tox120, RBL04199-250ppb-CC1,4 /1 9 /9 9
20 042099021
MeOH Blank
21 042099022
Tox120, RTL, 6M , G 1 ,4/19/99
22 042099023
Tox120, RTL, 7M, G 1 ,4/19/99
23 042099024
Tox120, RTL, 8M, G 1 ,4/19/99
24 042099025
Tox120, RTL, 9M, G 1 .4/19/99
25 042099026
Tox120, RTL, 10M , G 1 ,4/19/99
26 042099027
Tox120, R B L04199-250ppb-C C 2,4/19/99
27 042099028
MeOH Blank
.
28 042099029
Tox120, RTL, 11M, G 1 ,4/19/99
29 042099030
Tox120, RTL, 13M, G 1 ,4/19/99
30 042099031
Tox120, RTL, 14M, G 1 .4/19/99
.
31 042099032
Tox120, RTL, 15M , G 1 ,4/19/99
32 042099033
Tox120, R B L04199-250ppb-C C 3,4/19/99
33 042099034
MeOH Blank
34 042099035
Tox120, RTL, 17M , G 2 .4/19/99
35 042099036
TOX120, RTL, 18M, G 2 ,4/19/99
36 042099037
Tox120, RTL, 19M , G 2 ,4/19/99
37 042099038
Tox120, RTL, 20M , G 2 .4/19/99
38 042099039
Tox120, RTL, 21M , G 2 ,4/19/99
39 042099040
Tox120. RBL04199-250ppb-CC4, 4/19/99
40 042099041
MeOH Blank
41 042099042
Tox120, RTL, 22M , G 2 ,4/19/99
42 042099043
T 0X I2O, RTL, 23M . G 2 ,4/19/99
43 042099044
T 0X I2O, RTL, 24M , G 2 ,4/19/99
44 042099045
T0X I2O, RTL, 25M , G 2 ,4/19/99
45 042099046 T 0X I2O, RTL, 26M , G 2 .4/19/99
46 042099047
TOX120, RBL04199-250ppb-C C 5,4/19/99
47 042099048
MeOH Blank
48 042099049
T 0X I2O, RTL, 27M , G 2 .4/19/99
49 042099050
T 0X I2O, RTL, 29M , G 2 ,4/19/99
50 042099051
T 0X I2O, RTL, 30M , G 2 ,4/19/99
51 042099052
Tox120, RTL, 31 M , G 2 ,4 /19/99
52 042099053
T 0X I2O, RBL04199-250ppb-C C 6,4/19/99
53 042099054
MeOH Blank
54 042099055
T 0X I2O, RTL, 33M , G 3 ,4/19/99
55 042099056
T0X I2O, RTL, 34M , G 3 ,4/19/99
56 042099057
T 0X I2O, RTL, 35M , G 3 .4/19/99
57 042099058
TOXI20, RTL, 36M , G 3 ,4/19/99
58 042099059
T 0X I2O, RTL, 37M , G 3 .4/19/99
59 042099060
T 0X I2O, RBL04199-250ppb-C C 7,4/19/99
60 042099061
MeOH Blank
MS File
In let File
B o ttle
9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min ' 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9mln
9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min
. 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9mln
. 9MIN-COMBI7IS-POAA 9mln 9MIN-COMB17IS-POAA 9min 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9mln
9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min
9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min 9MIN-COMB17IS-POAA 9min
9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9mln 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA - 9mln 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min 9M IN-CO M BI7IS-PO AA. 9mln 9MIN-COMBI7IS-POAA . 9min 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9mln 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9mln 9MIN-COMBI7IS-POAA 9mln 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min
9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9mln
9MIN-COMBI7IS-POAA 9min
91 1 2 3 4 5 6 7 8 9 10 11. 91 12 13 14 15 16 81 92 17 18 19 20 21 82 92 22 . 23 24 25 83 92 26 27 28 29 . 30 84 92 31 32 33 34 35 85 92 36 37 38 39 86 93 40 41 42 43
44 87 93
Masslynx Sample List
Sample List: C:\MASSLYNX\rats2.PRO\Sam pleDB\042099c.SPL Printed: Tue Apr 20 16:51:50 1999
Page 2
File Nam e
61 042099062 62 042099063 63 042099064 64 042099065 65 042099066 6 6 042099067 67 042099068 6 8 042099069 69 042099070 70 042099071 71 042099072 72 042099073 73 042099074 74 042099075 75 042099076 76 042099077 77 042099078 78 042099079 79 042099080 80 042099081 81 042Q99082 82 042099083 83 042099084 84 042099085 85 042099086 8 6 042099087 87 042099088 8 8 042099089 89 042099090 90 042099091 91 042099092 92 042099093 93 042099094 94 042099095 95 042099096 96 042099097 97 042099098 98 042099099 99 042099100 1 0 0 042099101 1 0 1 042099102
1 0 2 042099103 103 042099104 104 042099105
F ile Text
Tox120, RTL, 38M , G 3 ,4/19/99 Tox120, RTL, 39M , G 3 .4/19/99 Tox120, RTL, 40M , G 3 ,4/19/99 Tox120, RTL, 4 1 M . G 3 .4/19/99 Tox120, RTL, 42M , G 3 ,4/19/99 Tox120, RBL04199-250ppb-CC8,4/19/99 M eOH Blank Tox120, RTL, 43M , G 3 ,4/19/99 Tox120, RTL, 45M , G 3 ,4/19/99 Tox120, RTL, 46M , G 3 ,4/19/99 Tox120, RTL, 47M , G 3 ,4/19/99 Tox120, RBL04199-250ppb-CC9,4/19/99 M eOH Blank Tox120, RTL, 65M , G 5 ,4/19/99 Tox120, RTL, 66M , G 5 ,4/19/99 Tox120, RTL, 73M , G 5 ,4/19/99 Tox120, RTL, 74M , G 5 ,4/19/99 Tox120, RTL, 75M , G 5 ,4/19/99 Tox120, RBL04199-250ppb-CC4,4/19/99 . MeOH Blank Tox120, RTL, 77M , G 5 .4/19/99 Tox120, RTL, 78M , G 5 ,4/19/99 Tox120, RTL, 79M , G 5 ,4/19/99 Tox120, RBL04199-250ppt>-CC5,4/19/99
MeOH Blank Tox120, RTL, 3M , G1-250ppt>-MS, 4/19/99 Tox120, RTL, 3M . G1-250ppb-M SD, 4/19/99 Tox120, RTL, 6M , G1-250ppb-MS, 4/19/99 Tox120, RTL, 6M , G1-250ppt>-MSD, 4/19/99 Tox120, RTL, 13M , G1-250ppb-MS, 4/19/99 Tox120, RTL, 13M , G1-250ppb-M SD, 4/19/99 Tox120, RBL04199-250ppb-CC6,4/19/99 M eOH Blank Tox120, R B L04199-H20Blk-1,4 /1 9 /9 9 Tox120, RBL04199-UvBlk-1,4 /1 9 /9 9 Tox120, RBL04199-5ppb-1,4 /1 9 /9 9 Tox120, RBL04199-10ppb-1,4/19/99 Tox120, RBL04199-25ppb-1,4 /1 9 /9 9 TOX120, RBL04199-50ppt>-1,4/19/99 TOX120, RBL04199-100ppb-1,4 /1 9 /9 9 Tox120, RBL04199-250ppb-1,4 /1 9 /9 9 Tox120, RBL04199-500ppb-1,4/19/99 Tox120, RBL04199-750ppt>-1,4 /1 9 /9 9 Tox120, RBL04199*1000ppb-1,4 /1 9 /9 9
MS File
In let File
9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min 9M IN-COMBI7IS-POAA Omln 9MIN-COMBI7IS-POAA 9min ' 9M IN-COMBI7IS-POAA 9min 9M IN-COMBI7IS-POAA 9mln 9M IN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9mln 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min 9M INtCOMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9mln 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9m!n 9MIN-COMBI7IS-POAA 9mjn 9M IN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min . 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA : 9min 9MIN-COMBI7IS-POAA 9mln 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min 9M IN-COM BI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9mln 9MIN-COMBI7IS-POAA 9min 9M1N-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9min 9MIN-COMBI7IS-POAA 9mln
B ottle
45 46 47 48 49
88
93 50 51 52 53 89 93
68
69 70 71 72 84 94 73 74 75 85 95 76 77 78 79 80 90
86
94 1.
2
3 4 5
6
7
8
9
10
11
Masslynx -Sample List
/
Sample List:C:\MASSLYNX\rats2.PRO\SampleDB\042099d.SPL Printed:Tue Apr2016:51:40 1999
.?i
i
FileName
FileText
1 042099106
MeOH Blank
I
2 042099107
Tox120, RBL04199-H2OBIk-1,4/19/99
i
3 042099108 4 042099109
Tox120, RBL04199-UvBlk-1,4/19/99 Tox120,RBL04199-5ppb-1,4/19/99
5 042099110
Tox120, RBL04199-10ppb-1,4/19/99
6 042099111
Tox120, RBL04199-25ppb-1,4/19/99
l 7 042099112 8 042099113
Tox120, RBL04199-50ppb-1,4/19/99 TOX120, RBL04199-100ppb-1,4/19/99
9 042099114
Tox120, RBL04199-250ppb-1,4/19/99
10 042099115
Tox120, RBL04199-500ppb-1,4/19/99
1 11 042099116 12 042099117
Tox120, RBL04199-750ppb-1,4/19/99 Tox120, RBL04199-1000ppb-1,4/19/99
13 042099118
MeOH Blank
14 042099119
TOX120, RTL, 49M, G4,4/19/99
1 15 042099120 16 042099121
TOX120, RTL, 50M, G4,4/19/99 Tox120, RTL, 51M, G4,4/19/99
17 042099122
TOX120, RTL, 52M, G4.4/19/99
T 18 042099123 19 042099124 20 042099125
Tox120, RTL, 53M, G4,4/19/99 TOX120, RBL04199-250ppb-CC1,4/19/99 MeOH Blank
21 042099126
Tox120, RTL, 54M, G4.4/19/99.
22 042099127
Tox120, RTL, 55M, G4.4/19/99
23 042099128
Tox120, RTL, 56M, G4,4/19/99
24 042099129
Tox120, RTL, 57M, G4,4/19/99
25 042099130
TOX120, RTL, 58M, G4,4/19/99
26 042099131
Tox120, RBL04199-250ppb-CC2,4/19/99
27 042099132
MeOH Blank
.
28 042099133
Tox120, RTL, 59M, G4,4/19/99
29 042099134
Tox120, RTL, 61M, G4,4/19/99
30 042099135
Tox120, RTL, 62M, G4,4/19/99
31 042099136
Tox120, RTL, 63M, G4,4/19/99
32 042099137
Tox120, RBL04199-250ppb-CC3,4/19/99
33 042099138
MeOH Blank
34 042099139 .Tox120, RBL04199-H2OBIk-1,4/19/99
35 042099140
Tox120, RBL04199-UvBlk-1,4/19/99
J 36 042099141 37 042099142 38 042099143
Tox120, RBL04199-5ppb-1,4/19/99 Tox120, RBL04199-10ppb-1,4/19/99 Tox120, RBL04199-25ppb-1,4/19/99
39 042099144
Tox120, RBL04199-50ppb-1,4/19/99
40 042099145
Tox120, RBL04199-100ppb-1,4/19/99 '
J 41 042099146 42 042099147
TOX120, RBL04199-250ppb-1,4/19/99 TOX120, RBL04199-500ppb-1,4/19/99
43 042099148
Tox120, RBL04199-750ppb-1,4/19/99
44 042099149
TOX120, RBL04199-1000ppb-1,4/19/99
- . MSFIle
y / ^ V B A C K TO MAIN
> Page 1
'
.
InletFile Bottle
longB
91
long6
1
long6
2
long6
3
longB
4
longB
5
longB
6
longB
7
longB
8
long6
9
!ong6
10
long6
11
longB
93
longB
54
long6
55
longB
56
long6
57
long6 . 58
long6
81
long6
94
longB
59
long6
60
long6
61
long6
62
long6
63
long6
82
long6
94
long6
64
long6
65
long6
66
longB
67
longB
83
long6
94
long6
.1
* longB
2
' long6
3
long6 '
4
long6
5
long6
6
longB
7
longB
8
long6
9
longB
10
long6
11
Masslynx - Sample List
i
Sample List:C:\MASSLYNX\rats2.PRO\SampleDB\042099d.SPL Printed:Tue Apr 2016:51:401999
. !|
I
File Nam e
FileText
1 042099106
MeOH Blank
l
2 042099107
Tox120, RBL04199-H20Blk-1,4/19/99
i
3 042099108
Tox120, RBL04199-UvBlk-1,4/19/99
4 042099109
Tox120, RBL04199-5ppb-1,4/19/99
5 042099110
Tox120, RBL04199-10ppb-1,4/19/99
6 042099111
TOX120, RBL04199-25ppb-1,4/19/99
l 7 ' 042099112 8 042099113
Tox120, RBL04199-50ppb-1,4/19/99 Tox120, RBL04199-100ppb-1,4/19/99
9 042099114
Tox120, RBL04199-250ppb-1,4/19/99
10 042099115
Tox120, RBL04199-500ppb-1,4/19/99
1 11 042099116 12 042099117
Tox120, RBL04199-750ppb-1,4/19/99 Tox120, RBL04199-1000ppb-1,4/19/99
13 042099118
MeOH Blank
14 042099119
TOX120, RTL, 49M, G4,4/19/99
l 15 042099120 16 042099121
TOX120, RTL, 50M, 64,4/19/99 Tox120, RTL, 51M, G4.4/19/99
17 042099122
Tox120, RTL, 52M, G4.4/19/99
18 042099123
Tox120, RTL, 53M, G4.4/19/99
19 042099124
TQX120, RBL04199-250ppb-CC1,4/19/99
I 20 042099125 21 042099126
MeOH Blank Tox120, RTL, 54M, G4,4/19/99.
22 042099127
Tox120, RTL, 55M, G4,4/19/99
23 042099128
Tox120, RTL, 56M, G4,4/19/99
24 042099129
Toxi20, RTL, 57M, G4,4/19/99
25 042099130
Tox120, RTL, 58M, G4,4/19/99
26 042099131
TOX120, RBL04199-250ppb-CC2,4/19/99
27 042099132
MeOH Blank
.
28 042099133
Tox120, RTL, 59M.G4,4/19/99
29 042099134
Tox120, RTL, 61M, G4,4/19/99
30 042099135
TOX120, RTL, 62M, G4,4/19/99
31 042099136
TOX120, RTL, 63M, G4,4/19/99
32 042099137
TOX120, RBL04199-250ppb-CC3,4/19/99
33 042099138
MeOH Blank
34 042099139 .TOX120, RBL04199-H20Blk-1,4/19/99
35 042099140
Tox120, RBL04199-UvBlk-1,4/19/99
J 36 042099141 37 042099142 38 042099143
TOX120, RBL04199-5ppb-1,4/19/99 Tox120, RBL04199-1Oppb-1,4/19/99 Tox120, RBL04199-25ppb-1,4/19/99
39 042099144
Tox120, RBL04199-50ppb-1,4/19/99
40 042099145
TOX120, RBL04199-100ppb-1,4/19/99 ~
1 41 042099146 42 042099147
Tox120, RBL04199-250ppb-1,4/19/99 TOX120, RBL04199-500ppb-1,4/19/99
43 042099148
Tox120, RBL04199-750ppb-1,4/19/99
44 042099149
TOX120, RBL04199-1000ppb-1,4/19/99
]
I
l
!I
y / ^ BACK TO MAIN
- , Pagel
'
'.
/ MS File PFBS-4 PFBS-4 PFBS-4 PFBS-4 PFBS-4 PFBS-4 PFBS-4 PFBS-4
I PFBS-4 I PFBS-4
PFBS-4 ' PFBS-4
PFBS-4 PFBS-4 PFBS-4 PFBS-4 PFBS-4 ' . PFBS-4 PFBS-4 , .PFBS-4 / PFBS-4 PFBS-4 PFBS-4 PFBS-4 PFBS-4 PFBS-4 PFBS-4 PFBS-4 PFBS-4 PFBS-4 . PFBS-4 PFBS-4 PFBS-4 PFBS-4 PFBS-4 , PFBS-4 \ PFBS-4 \ PFBS-4 \ PFBS-4 1 PFBS-4 I PFBS-4 \ PFBS-4 \ PFBS-4 \ PFBS-4
\ '
InletFile
Bottle
long6
91
long6
1
longe
2
long6
3
long6
4
longe
5
long6
6
long
7
longe
8
longe
9
long6
10
long6
11
longe
93
longe
54
longe
55
longe
56
longe
57
long6 . 58
longe
81
longe
94
long6
59
long6
60
long6
61
long6
62
long6
63
longe
82
long6
94
long6
64
long6
65
longe
66
longe
67
long6
83
long6
94
longe
.1
*longe
2
s long6
3
long6 '
4
long6
5
long6
6
long6
7
longe
8
long6
9
longe
10
long6
11
] 1
J ]
J
Scanning Method Saport
M ethod!
e:\MMSUMX\SAIS2.PltO\hCgDDS\9MZH-CCMBXTZS-MMh
laat-Modified: Tue Apr 20 1:33:12 1999
'
Printed!
Tue Apr 20 16i33:19 1999
Solvent Delay ( Mina ) !
0.00
Function ! 1
MRM of 1 Maas Pair ( ISP- )
Inter Channel Delay ( Sue* ) t
Span ( Deltona ) i
Start Tie* ( Hina ) i
Cnd Tina ( Mina ) >
Repeata 1
.
Channel Parent D.ught.r
0.03 0.00 0.00 9.00
1 Dwell
(Sk i ) Coll Inorgy
(V)
COM
`1
413.00 169.00 0.30
30
1$
Function : 2
MRM of 1 M u i Pair ( ISP- |
Inter Chennai Daley ( Sacs ) t Span ( Deltona ) * Start Tina { Mins ) * End Tina ( Mins ) t Repeats t
Chennai Parent Daughter
0.03 0.00 0.00 9.00
1 Dwell
(Sac*)
.
Coll Snargy
(V)
Con.
1 469.00 119.00 0.30
30
13
Function i 3
MSN of 1 Maaa Pair ( ISP- )
Inter Chennai Daley ( S.c. ) Span ( Deltona ) Start Tina { Mina ) : End Tina { Mina ) * Rapaats ;
Chennai Parent Daughter
0.03 0.00 0.00 9.00
1 Dwell
(Seca) Coll In.rgy (V)
Con*
1 569.00 119.00 0.30
30
IS
Function : 4
MRM of 1 Maes Pair ( ESP- )
Intar Chennai Delay ( S.C* ) ! Span ( Daltons ) : Start Tina ( Mina ) s End Tima ( Kina ) : Repeats t
Chennai Parent D.ught.r
0.03 0.00 0.00 9.00 1 Dwell
(S.c*) Coll tnargy
(V)
Con.
1 419.00 119.00 0.30
30
15
Function r 5
MRM of 1 Mess Pair ( ZIP- )
Inter Chennai Delay ( Secs ) t Span ( Daltons ) t
Start Tima { Mins ) : End Tima { Hina ) j Rapaats i
Channel Parent Daughter
0.03 0.00 0.00 9.00 1 Dwell
(S.C.)
Coll Energy
(V)
Co m
1 S19.00 119.00 0.30
30
Funestion z C ' MRM of 1 Mass Pair ( U P - )
IS -
Inter Channel Delay ( Secs ) t
Span ( Daltons ) :
Start Time ( Mina ) i End Time ( Mina ) 1 Repeats t
Channel Parent Daughter
0.03 0.00 0.00 9.00 1 Dwell
(Sua)
Coll In.rgy
(V)
Co m
1
499.00 99.00*
0.30
43
*0
Function t ?
MRM of 1 Mass Pair ( ISP- )
Inter Channel Delay ( 3ca ) ! Span ( Daltons ) s Start Time < Mins ') s End Tine ( Mina ) i Repeats i
Channel Parent Daughter
0.03 0.00 0.40 9.00 1 Dwell
(Sial Coll Enargy <VJ
Con.
1
399.00 0.00
0.30
43
60
Function i 1
MRM of 1 Maas Pair { u p - )
Inter channel Delay ( Sacs ) t Span ( Daltons ) t Start Tims ( Mina } : End Tims ( Mina ) t
Repeats s Channel Parent Daughter
0.03 0.00 0.00 9.00 1 Dwell
(S.C*) Coll Enrgy (V)
Con.
1
427.00 60.00
0.30
40
60
7 / * BACK TO MAIN
Page 1
Scanning Method Report
Mathod:
C :\MASSLYXX\RATS2. PRO\ACQUCB\9KIN-CCMBI7IS-POAA
Laat"Modifiad: Tua Apr 20 16:33:12 1999
Printad:
Tua Apr 20 16:33:19 1999
Solvant Delay ( mina ) i
0.00
Function 1 1
M M of 1 M u a Pair ( ISP- )
Intar Channal Dalay ( Sacs ) t Span ( Daltons } : Start Tima ( Hina ) : End Tima ( Hina ) : Repasta i
Channal Parent Daughter
0.03 0.00 0.00 9.00
1 Dwell
(Sacs) Coll Energy (V)
Cona ( V )
*1
413.00 169.00 0.30
30
15
function : 2
MKM of 1 Haas Pair I ISP- )
Inter Channal Dalay ( Secs ) t Span ( Daltons ) t Start Time ( Mina ) : End Tima ( Mina ) : Repeats :
Channal Parent Daughter
0.03
0.00 0.00 9.00
1 Dwell
(Sacs)
.
Coll Energy
(V)
Cona ( V )
1 469.00 119.00 0.30
30
15
function : 3
M M of 1 Mass Pair ( ESP- |
. -
Inter Chann.1 Dtlay ( Secs ) s Spin ( Dalton* ) I Start Tin* ( Hina ) I End Tin* l Mina ) i Repeats !
Channal Parent Daughter
0.03 0.00 0.00 9.00
1 Dwell
(Sacs) Coll Energy
leV)
Cona ( V )
1 569.00 119.00 0.30
30
15
function : 4
M M of 1 Maas Pair ( ESP- )
Intar Channal Dalay ( Sacs ) t Span ( Daltons ) : start Tima ( Hina ) : End Tima ( Mins ) t Repeats :
Channel Parent Daughter
0.03 0.00 0.00 9.00 1 Dwell
(Secs)
Coll Energy
(V)
Cona ( V J
1 419.00 119.00 0.30
30
15
function : 5
MRM of 1 M u a Pair ( ESP- |
Inter Channel Delay ( Sac* ) i Span ( Daltons ) : Start Time ( Kins ) : End Time ( Mins ) : Repeats :
Channel Parent Daughter
0.03 0.00 0.00 9.00 1 Dwell
(Secs) Coll Energy
(*V)
Cona ( V )
1 519.00 119.00 0.30
30
j
Function : C ' MRM of X M u e Fair ( ESP- )
15
Inter Channel Delay ( Sacs ) s Span { Daltons ) : Start Tima ( Mins ) : End Tima ( Mins ) : Repeats :
Channal Parent Daughter
0.03 0.00 0.00 9.00 1 Dwell
(Sees)
Coll Energy
(*V)
Cona ( V )
1
499.00 99.00
0.30
45
60
Function i 7
M M of 1 Maaa Pair ( ESP- J
Inter Channal Dalay ( Sacs ) t Span ( Daltons ) : Start Tia* ( Hina ') t End Tlaa ( Kins ) > Rapaats t -
Channal Parent Daughter
0.03 0.00 0.00 9.00 1 Dwell
(Secs) Coll Energy (*V)
Cona ( V )
1 399.00 80.00
0.30
45
60
Function i 8
M M of 1 Mnas Pair ( ISP- )
Inter Channal Delay ( Sacs ) { Span ( Dalton* ) : Start Tin* ( Mins ) : End Tina ( Min* ) I Repeats t
Channal Parant Daughter
0.03 0.00 0.00 9.00
1 Dwell
(Secs) Coll Energy
(V)
Cona ( V )
1
427.00 80.00
0.30
40
60
.. /
" BACK TO MAIN
* Pag* 1 ' '
A
'1
canning Mathod R eport
Matho d :
C :\M M SH IK \R A T a2.FR O \A C Q O O S\
L aaJ Modif i ad : Non Mar IS 14:22:03 1999
l>rintad:
Tua Apr 20 17:03:29 1K 9
-4
-
lolvint Dalay ( Kina ) 1
0.00
funetien : 1
MRM of 4 Maaa valra ( H P - )
Intar Channal Dairy ( Sacs ) : Span ( Daltons ) : Start Tina (Mina ) *1 End Tina ( Mina ) 2 Rapeata :
Chahnal Parent Daughtar
0.03 0.00 0.00
13.SO
1 Duali (Sacs) Coll Energy (aV)
Con. (V )
*1 2 3
299.00 *80.00 299.00 99.00
299.00 130.00
0.30 0.30 0.30
45 45 45
60 60 60
y BACK TO MAIN
* Paga I
AMELIA062498: FACT Equipment Log 99016
Q uattro II, #6448E
BACK TO MAIN
/yu?&
PAGE
DATE: 04/22/99
.
ANALYST: mee
'
STUDY NUM BER Tox-120 M ATRIX: R at Liver DESCRIPTION: ip
FACT M ethod: ETS-8-S.0________
Injection volum e, lO uL
Cycle tim e: 13.5 m in.
M obile Phase:
(A)2.0 m M NH4CH3CQ2
Electrode: Z-Spray (B)M cO H
TN-A- or stndrd num ber (A) 99012-5-22_________
(B) TN-A-2414
Prepared (date/by):
(A) 4/16/99 / ok_________
(B j 4/9/99 hoi
Expires:
(A) 10/99_______________
(B) 10/99________
Store at room tem p? [YJ or N
O ther
G u ard colum n: N one __________________
Part number: na
Lot num ber na
A nalytical colum n: keystone betasil____________________________________________ Size: 2 x 50mm_________________ Type: c !8____________ Sphere size: 5 um Part num ber 055-701-2 ' L ot n um ber P7L04 Serial num ber 0481172z
A nalytical p aram eters:
t
Drying gas (L/hr): 300
_
ESI nebulizing gas (L/hr) 15
LC Flow rate: 300 uL/m in. Back pressure: 59 bar
.
H PLC solvent g rad ien t: long2
Tim e (m in.) 1) 0.00____________
2) 8.5_____________
.3 11.0____________
5) 12.0____________
6) 13.5_________
%B 40 90 90 40
stop
Sheath gas (X/hr) 0
BACK TO MAIN
<//**/??
_
AMELIA062498: FACT Equipment Log 99016
Q uattro H , #6448E
PAGE______
DATE: 04/22/99
ANALYST: mee
'
.
STUDY NUMBER Tox-120 MATRIX: R at Liver (pfbs) DESCRIPTION: ip
FACT M ethod: ETS-8-S.0________
Injection volum e,10 uL
Cycle time: 15 min. Electrode: Z-Spray
M obile P hase:
(A)2.0 mM NH4CH3CQ2
(B)M eO H
TN-A- or stndrd num ber (A) 99012-5-22_________
(B) TN-A-2414
Prepared (date/by):
(A) 4/16/99 / ok_________
' (B) 4/9/99 hoi
Expires:
(A) 10/99_______________
(B) 10/99
Store at room tem p?' [Y] or N
O th er:__
G u ard colum n: None '_________________
Part number: na
Lot number: na
A nalytical colum n: keystone betasil___________________________ .________________
Size: 2 x 50mm_________________ Type: c !8____________ Sphere size: 5 um
Part number: 055-701-2
Lot number: P7L04 Serial number: 0481172z
A nalytical param eters:
Drying gas (L/hr): 300
ESI nebulizing gas (L/hr) 15
LC Flow rate: 300 uL/min. Back pressure: 59 bar
Sheath gas (L/hr)_0
H PLC solvent g rad ien t: long2
Time (m in.)
%B
1) 0.00_______________ 25
2) 5.0_____________
95
3 12.0
95
5) 13.5
25
6) 15__________
stop
BACK TO MAIN
/ > 0 t?
Tuning Method Report
.
Method:
C :\MASSLYNX\RATS2. PRO\ACQUDB\PFOS
Printed:
Thu Apr 22 16:25:24 1999
AiwJfc OLMI
Page 1
lA t o ilh ill
SOURCE ( ESP- )
Capillary Cone Extractor RF Lens Source Block Temp. Desolvation Temp.
Set Rdbk
3.00 15 5 .0.2 150 250
2.96 -14 -5 -
152 253
MSI
LM Resolution HM Resolution - Ion Energy Ion Energy Ramp Lens 6 Multiplier
Pressures
Analyser Vacuum Gas Cell
Rdbk
. 2.0e-4 5.5e-3
.
.s
MS2
LM Resolution HM Resolution Collision Ion Energy Ion Energy Ramp Lens 8 Lens 9 Multiplier
Set
' 15.0 15.0 2 -0 $ 0.0 . 5 650
Set Rdbk
15.0 15.0 . 30 32 2.0 0.0 40 39 0 31 650
BACK TO MAIN
Masslynx Sample List Sample List C:\MASSLYNX\rats2.PRO\SampleDB\042299a.SPL Printed:Tilt.Apr22 17:50:31 1999
,
'
Idiilit, ftf,
Page 1
File N a m e
FileText
.
1 042299001
MeOH Blank
2 042299002
Tox120, RBL04199-H20Blk-1,4/19/99
3 042299003
Tox120, RBL04199-LivBlk-1,4/19/99
4 042299004
Tox120, RBL04199-5ppb-1,4/19/99
5 042299005
Tox120, RBL04199-10ppb-1,4/19/99
6 042299006
Tox120, RBL04199-25ppb-1,4/19/99
7 042299007
Tox120, RBL04199-50ppb-1,4/19/99
8 042299008
Tox120, RBL04199-100ppb-1,4/19/99
i
9 042299009
Tox120, RBL04199-250ppb-1,4/19/99
10 042299010
Tox120, RBL04199-500ppb-1,4/19/99
11 042299011
Tox120, RBL04199-750ppb-1,4/19/99
12 042299012
Tox120, RBL04199-1000ppb-1,4/19/99
13 042299013
MeOH Blank
14 042299014
Tox120, RTL, 49M, G4,4/19/99, D10
15 042299015
Tox120, RTL, 50M, G4,4/19/99, D10
16 042299016
Tox120, RTL, 51M, G4,4/19/99, D10
17 042299017
Tox120, RTL, 52M, G4,4/19/99, D10
18 042299018
Tox120, RTL, 53M, G4,4/19/99, D10
19 0422990-19
Tox120, RBL04199-250ppb-CC1,4/19/99 .
20 042299020
MeOH Blank
21 042299021
Tox120, RTL, 54M, G4.4/19/99, D10
22 042299022
Tox120, RTL, 55M, G4,4/19/99, D10
23 042299023
Tox120, RTL, 56M, G4,4/19/99, D10
24 042299024
Tox120, RTL, 57M, G4,4/19/99,
-
25 042299025
Tox120, RTL, 58M, G4,4/19/99,
26 042299026
Tox120, RBL04199-250ppb-CC2,4/19/99
27 042299027
MeOH Blank
-
28 042299028
Tox120, RTL, 59M, G4,4/19/99,
29 042299029
Tox120, RTL, 61M.G4,4/19/99,
30 042299030
Tox120, RTL, 62M, G4,4/19/99, .
31 042299031
Tox120, RTL, 63M, G4,4/19/99,
32 042299032
Tox120, RBL04199-250ppb-CC1,4/19/99
33 042299033
MeOH Blank -
34 042299034
Tox120, RBL04199-H2OBIk-1,4/19/99
35 042299035
Tox120, RBL04199-UvBlk-1,4/19/99
36 042299036
Tox120, RBL04199-5ppb-1,4/19/99
37 042299037
Tox120, RBL04199-10ppb-1,4/19/99
38 042299038 . Tox120, RBL04199-25ppb-1,4/19/99
39 042299039
Tox120, RBL04199-50ppb-1,4/19/99
40 042299040
Tox120, RBL04199-100ppb-1,4/19/99
41 042299041
Tox120, RBL04199-250ppb-1,4/19/99
42 042299042
Tox120, RBL04199-500ppb-1,4/19/99
43 042299043
Tox120, RBL04199-750ppb-1,4/19/99
44 042299044
Tox120, RBL04199-1000ppb-1,4/19/99
M S File
15MIN 15MIN 15MIN 15MIN 15MIN 15MIN 15MIN 15MIN 15MIN 15MIN 15MIN 15MIN 15MIN 15MIN 15MIN 15MIN 15MIN 15M1N 15MIN 15MIN 15MIN 15MIN 15MIN 15MIN 15MIN 15MIN 15MIN 15MIN 15MIN 15MIN 15MIN 15MIN 15MIN 15MIN 15MIN 15MIN 15MIN 15MIN
15M1N
15MIN 15MIN 15MIN 15M1N 15MIN
InletFile Bol
long15 long15 long15 long15 long15 long15 long15 long15 long15 long15 long15 long15 long15 long15 long15 long15 long15 long15 long15 long15 long15 long15 long15 long15 long15 long15 long15 long15 long15 long15 long15 long15 long15 long15 a long15 long15 long15 long15 long15 long15 long15 long15 long15 long15
91 1 2 3 4 5 6 7 8 9 10 11 91 12 13 14 15 16 17 91 18 19 20 21 22 23 91 24 25 26 27 17 92 1, 2 3 4 5 6 -7
8
9 10 11
y/z i
BACK TO MAIN
Masslynx Sample List
Sample List:C:\MASSLYNX\rats2.PRO\SampleDB\042299b.SPL
Printed:Thu Apr2217:46:29 1999
4^
. Page 1
M C b i / b / i l ___________________ ____
File N a m e
FileText
MS File
InletFile
1 042299045
MeOH Blank
13MIN-COMBI7IS-POAA long2
2 042299046
Tox120, RBL04199-H2OBIk-1,4/19/99
13MIN-COMBI7IS-POAA long2
3 042299047
Tox120, RBL04199-UvBlk-1,4/19/99
13MIN-COMBI7IS-POAA long2
4 042299048
Tox120, RBL04199-5ppb-1,4/19/99
13MIN-COMBI7IS-POAA long2
5 042299049 . Tox120, RBL04199-10ppb-1,4/19/99
13MIN-COMBI7IS-POAA long2
6 042299050
Tox120, RBL04199-25ppb-1,4/19/99
13MIN-COMBI7IS-POAA long2
7 042299051
Tox120, RBL04199-50ppb-1,4/19/99
13MIN-COMBI7IS-POAA long2
8 042299052
Tox120, RBL04199-100ppb-1,4/19/99
13MIN-COMBI7IS-POAA long2
9 042299053
Tox120, RBL04199-250ppb-1,4/19/99 .
13MIN-COMBI7IS-POAA long2
10 042299054
Tox120, RBL04199-500ppb-1,4/19/99
13MIN-COMBI7IS-POAA long2
11 042299055
Tox120, RBL04199-750ppb-1,4/19/99
13MIN-COMBI7IS-POAA long2
12 042299056
Tox120, RBL04199-1000ppb-1,4/19/99
13MIN-COMBI7IS-POAA long2
13 042299057
MeOH Blank
13MIN-COMBI7IS-POAA long2
14 042299058
Tox120, RTL, 17M, G2,4/19/99
13MIN-COMBI7IS-POAA long2
15 042299059
Tox120, RTL, 18M, G2,4/19/99
13MIN-COMBI7IS-POAA long2
16 042299060
Tox120, RTL, 19M, G2,4/19/99
13MIN-COMBI7IS-POAA long2
17 042299061
Tox120, RTL, 20M, G2,4/19/99
13MIN-COMBI7IS-POAA long2
18 042299062
Tox120, RTL, 21M, G2,4/19/99
13M IN-COMBI7IS-POAA long2
19 042299063
Tox120, RBL04199-250ppb-CC4,4/19/99 .
13MIN-COMBI7IS-POAA long2
20 042299064
MeOH Blank
13MIN-COMBI7IS-POAA long2
21 042299065
Tox120, RTL, 22M, G2,4/19/99
13MIN-COMBI7IS-POAA long2
22 042299066
Tox120, RTL, 23M, G2,4/19/99
13MIN-COMBI7IS-POAA long2
23 042299067
Tox120, RTL, 24M.G2,4/19/99
.
13MIN-COMBI7IS-POAA long2
24 042299068
Tox120, RTL, 25M, G2,4/19/99
13MIN-COMBI7IS-POAA long2
25 042299069 26 042299070
Tox120, RTL, 26M.G2,4/19/99 Tox120, RBL04199-250ppb-CC5,4/19/99
13MIN-COMBI7IS-POAA long2 13MIN-COMBI7IS-POAA long2
27 042299071
MeOH Blank
.
13MIN-COMBI7IS-POAA long2
28 042299072
Tox120, RTL, 27M, G2,4/19/99
13MIN-COMBI7IS-POAA long2
29 042299073
Tox120, RTL, 29M, G2, 4/19/99
13MIN-COMBI7IS-POAA long2
30 042299074
Tox120, RTL, 30M, G2.4/19/99
13M IN-COMBI7IS-POAA long2
31 042299075
Tox120, RTL. 31M, G2,4/19/99
13MIN-COMBI7IS-POAA long2
32 042299076
Tox120, RBL04199-250ppb-CC6,4/19/99
13M IN-COMBI7IS-POAA long2
33 042299077
MeOH Blank
13MIN-COMBI7IS-POAA long2
34 042299078
Tox120, RTL, 33M, G3,4/19/99
13MIN-COMBI7IS-POAA long2
35 042299079
Tox120, RTL, 34M, G3,4/19/99
13MIN-COMBI7IS-POAA long2
36 042299080
Tox120, RTL, 35M, G3,4/19/99
13MIN-COMBI7IS-POAA long2
37 042299081
Tox120, RTL, 36M, G3,4/19/99
13MIN-COMBI7IS-POM long2
38 042299082
Tox120, RTL, 37M.G3,4/19/99
13MIN-COMBI7IS-POAA long2
39 042299083
Tox120, RBL04199-250ppb-CC7, 4/19/99
13M IN -COM BI7IS-POAA long2
40 042299084
MeOH Blank
. 13MIN-COMBI7IS-POAA long2
41 042299085
Tox120, RTL, 38M, G3,4/19/99
13MIN-COMBI7IS-POAA long2
42 042299086
Tox120, RTL, 39M, G3,4/19/99
13MIN-COMBI7IS-POAA long2
43 042299087
Tox120, RTL, 40M, G3,4/19/99
13MIN-COMBI7IS-POAA long2
44 042299088
Tox120, RTL, 41M, G3,4/19/99
13MIN-COMBI7IS-POAA long2
45 042299089 Tox120, RTL, 42M, G3,4/19/99
13MIN-COMBI7IS-POAA long2
46 042299090
Tox120, RBL04199-250ppb-CC8,4/19/99
13MIN-COMBI7IS-POAA long2
47 042299091
MeOH Blank
13MIN-COMBI7IS-POAA long2
48 042299092
Tox120, RTL, 43M, G3.4/19/99
13MIN-COMBI7IS-POAA long2
49 042299093
Tox120, RTL, 45M, G3,4/19/99
13MIN-COMBI7IS-POAA long2
50 042299094
T0XI2O, RTL, 46M, G3,4/19/99
13MIN-COMBI7IS-POAA long2
51 042299095
Tox120, RTL, 47M, G3,4/19/99
13MIN-COMBI7IS-POAA long2
52 042299096
Tox120, RBL04199-250ppb-CC9,4/19/99
13MIN-COMBI7IS-POAA long2
53 042299097
MeOH Blank
13MIN-COMBI7IS-POAA long2
54 042299098
Tox120, RBL04199-H20Blk-1,4/19/99
13MIN-COMBI7IS-POAA long2
55 042299099
Tox120, RBL04199-LivBIk-1,4/19/99
13MIN-COMBI7IS-POAA long2
56 042299100
Tox120, RBL04199-5ppb-1,4/19/99
* 13MIN-COMBI7IS-POAA long2
57 042299101
Tox120, RBL04199-10ppb-1,4/19/99
13MIN-COMBI7IS-POAA long2
58 042299102
Tox120, RBL04199-25ppb-1,4/19/99
13MIN-COMBI7IS-POAA long2
59 042299103
Tox120, RBL04199-50ppb-1,4/19/99
13MIN-COMBI7IS-POM long2
60 042299104
Tox120, RBL04199-100ppb-1,4/19/99
13MIN-COMBI7IS-POAA long2
Bottle
91 1 2 3 4 5 6 7 8 9 10 11 91 31 32 33 34 35 84 92 36 37 38 39 40 85 92 41 42 43 44 86 93 45 46 47 48 49 87 93 50 51 52 53 54 88 93 55 56 57 58 89 93 1 2 3 4 5 6 7
Masslynx Sample Ust
Sample List:C:\MASSLYNX\rats2PRO\SampleDB\042299b.SPL
Printed:Thu Apr2217:46:29 1999
A Amliz
Q L 2M
BACK TO MAIN
z?
Page 2
Me or/itjw
File Name
61 042299105 62 042299106 63 042299107 64 042299108
66 67
68
69 70 71 72 73 74
FileText
Tox120, RBL04199-250ppb-1,4/19/99 Tox120, RBL04199-500ppb-1,4/19/99 Tox120, RBL04199-750ppb-1,4/19/99 Tox120, RBL04199-1000ppb-1,4/19/99
M S File
InletFile
13MIN-COMBI7IS-POAA long2 13MIN-COMBI7IS-POAA long2 13MIN-COMBI7IS-POAA long2 13MIN-COMBI7IS-POAA long2
Bottle 8 9 10 11
BACK TO MAIN
Scanning Method Report
Method:
C:\MASSLTHX\RAT82.PRO\ACQUDB\13MZM-CCMBZ7ZS-POAA
Last Modified! Thu Apr 22 18:50:10 1999
.
Printed:
Thu Apr 22 18:50:18 1999
eJn
Solvant Daisy ( aina ) :
0.00
function : 1
MRH of 1 Mass Pair ( ISP- )
Intar Channel Delay ( Sacs ) i Span ( Daltons ) Start Tint I Mins ) t End Time ( Mins ) :
Repeats t Channel Parent Daughter
0.03 0.00 0.00
13.50
1
Dwell
(Secs)
Coll Energy
(aV)
Cone ( V )
413.00 169.00 0.30
function : 2
MRM of 1 Maes Pair ( ESP- )
Inter Channel Delay ( Secs ) : Span ( Daltons ) : Start Time ( Mins ) : End Time ( Mins ) : Repeats :
Channel Parent Daughter
0.03 0.00 0.00
13.50
1
Dwell
.
(Secs) Coll Energy (eV)
*
Cone ( V )
1 469.00 119.00 0.30
45 ' 35
function : 3
MRM of 1 Maas Pair ( ESP- )
Inter Channel Delay ( Secs ) t Span ( Daltons } :
Start Time ( Mins ) : End Time ( Mins ) * Repeats i
Channel Parent Daughter
0.03 0.00 0.00
13.50
1
Dwell (Secs) Coll Energy (eV)
Cone ( V )
1 569.00 119.00 0.30
45
35
function s 6
M m of 1 Mass Pair t EBP- )
Inter Channel Delay ( Sacs ) i Span ( Daltons ) : Start Time ( Mins } ; End Time ( Mins ) : Repeats t
Channel Parent Daughtsr
0.03 0.00 0.00 13.50
Dwell (Secs) Coll Energy
(eV)
Cone ( V
1 419.00 119.00 0.30
45
35
function : 5
MRM of 1 Mass Pair ( E8P- )
Intsr Channel Delay ( Secs ) :
Span { Daltons ) :
Start Tiae ( Mins ) :
End Time ( Mins ) : Repeats :
*
Channel Parent Daughter
0.03
0 .0 0 0.00
13.SO
1
Dwell
(5ecs)
*
Coll Energy
(eV)
i 519.0 119.00 0.30
45
Cone ( V ) 35
function : 6 * MRM of 1 Mass Pair ( ESP- )
Inter Channel Delay ( Seca ) : Span ( Daltons ) : Start Time ( Mins ) t End Time ( Mins ) : Repeats i
Channel Parent Daughter
0.03 0.00 0.00 13.50
1 Dwell (Secs) Coll Energy tv>
Cone
1
499.00 99.00*
0.30
45
80
Function > 7
M M of 1 M... Pair ( ESP- )
Inter Channel Delay ( Secs ) t Span ( Daltons ) st Start Time ( Mins ) t End Time ( Mins ) t
Repeats : Channel Parent Daughter
0.03 0.00 0.00 13.50 1 Dwell
(Secs) Coll Energy
(V)
Cone
1
399.00 60.00
0.30
45
80
Function i
M M of 1 H U I Pair ( ESP- )
Inter Channel Delay ( Secs ) : Span ( Daltorn ) : Start Time ( Mina ) j End Time ( Mins ) : Repeats :
Channel Parent Daughter
0.03 0.00 0.00 13.50 1 (Veil
(Sec*) Coll Energy
(VI
Cone
1
427.00 80.00
0.30
40
60
Q i t W L ______
or.ifJit
3?
Pag* 1
Scanning Mathod Report
_-
Method:
C :\HAS8LYNX\RAT82. FSO\ACQUDB\15MXM
Last Modified: Thu Apr 22 15:32:30 1999
Printed:
Thu Apr 22 15:52:34 1999
-4
nvlA tLViV
Solvent Delay ( nine ) :
0.00
Function t 1
MRM of 4 Mass Pairs C ESP- ) *
Intar Channal Oalay ( Secs ) : Span ( Daltons ) i Start Time { Mins ) : End Time ( Mins ) : Repeats :
Channel Parent Daughter
0.03 0.00 0.00 15.00 1 Dwell
(Secs) Coll Energy (eV)
Cone ( V )
1 2 3 .4
299.00 299.00 299.00 299.00
80.00 99.00 130.00 180.00
0.30 0.30 0.30 0.30
45 45 45 45
60 60 60 60
TM BACK TO MAIN
rf/^/yy
L sh M __________
Pag. 1
]
Scanning Method Report
Method!
Cl\MASSI.ln0C\RATS2.PRO\AC0UDB\l
l u t M odified: Thu Apr 22 19>52:S0 199
P rin te d :
Thu Apr 22 15i52:54 1999
Amelia nLM/tt
! Solvent Daisy ( Bins ) I
0.00
Punatlea i 1
M M of 4 Maaa P a i n ( U P - ) '
Intar Channal Dalay ( Sacs ) i Span ( Daltona ) Start T i n ( Mina ) i End Tlaa ( Mina ) t Aapaata :
Channal Parant Daughter
0.03
'
0.00
o.oo
15.00
1
Dwell (Steal coll Energy (aV)
Cone ( V )
1
299.00 80.00
0.30
2
299.00 99.00
0.30
3 299.00 130.00 0.30
4 299.00 180.00 0.30
45 45 45 45
80 0 0 60
" " BACK TO MAIN
rf/^/yy
Pug* 1
LAC, O x / m / t f
' ' BACK TO MAIN
I FACT: DATA REVIEW SUMMARY FORM
1 4/3/$9
.
1
I
]
3
1
T
J
J
1
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D oJfiU
j ( a . c A u / m / a - j loo f x j U - A ^ y i i / d h i ^ r e c t ^
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f / 2 .5 / 3 3
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1
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1
1
1
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]
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J
I
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FACT: DATA REVIEW SUMMARY FORM
4/2-3/99
BACK TO MAIN '
*2* O x m a m , , t o hx. pl&Jrt n h y r tc fa A .
V W s
fcjv- 4 / 2 - 5 / 3 3
/
\
Amila 062498: Raviavad and quantitatad by kjh 4/23/99
f
i
03
> O *
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>
Quantify Calibration Raport
Calibration: C:\MMSLTOC\rata2.PRO\CurvaD8\04229ta
Last nodifiad: Frl Apr 23 08:51:34 1999
Prlntad:
frl Apr 23 08:52:40 1999
'
"
BACK TO MAIN
, Vaga 1 . '
Amalia 062498: Ravlawad and quantittad by k)h 4/23/99
BACK TO MAIN
] !
M atta# R epert
M attala ( H a i Laet n a tifla ti
eaaVe w i ^ -
frltta # i
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Cm m . ( RM4rt*i
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t a t t i n t * h m m f a e te ri
1.000000a 1 .0 0 0 0 0 0 , M a t t i a #
1.00
t" >*> .
IttN I tlM TSM IS lM ll M U U n R e ta tila T IM I t e MlrtMw l a i M l i
-
teak M m U m i
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9ttmaav\m
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tuUaaoUfi
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R e la tiv a R alfO ti M ie la te M io tti R e lativ a a rm i M ia lu ta a rm i
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t
A ivJ/A Ms ZH W /a c . or//ft/ l _______ M
H* l
SOUP 020199: FACT Equipment Log 99014
Q uattro II, #6414E
PAGE
DATE: 05/14/99
..
ANALYST: drb
'
STUDY NUMBER: Tox-120 MATRIX: R at L iver DESCRIPTION: dilutions FACT M ethod: ETS-8-5.0 .
Injection volume: 10 uL
Cycle time: 9 min. Electrode: Z-Sprav______
M obile Phase:
(A12.Q mM NH4CH3CQ2
TN-A- or stndrd number (A) 99011-6-9__________
Prepared (date/by):
(A)04/28/99 drb________
Expires:
(A) 10/99 ________ _
OBI MeOH
(B) TN-A-2506
(Bl 04/26/99 drh fBl 10/99
Store at room temp? 0 or N
Other:________ ;__________._____
G u ard colum n: None__________________
P art number: na_________
Lot number: na________________________
A nalytical colum n: Keystone B etasil____________________________________
Size: 2 x 50 mm
___________ Type: C18____________ Sphere size: 5 um______
Part number: 055-701-2__________ Lot num ber P7LQ4
Serial number:0481175Z
A nalytical param eters:
Drying gas (L/hf): 350
ESI nebulizing gas (L /hr) 15
LC Flow rate: 3 0 0 11T/m in. Back pressure: 55 bar
Sheath gas (L/hr)_0
H PLC solvent gradient: 9m in-2
Time /m in.l
D 0.00__________ 2 1 1.00______________
3 ) A5Q___________
4) JL5Q__________
%B___________
4 0 Q ____________ j JOO________
J 1 0 _______
2 1 0 _______
.
BACK TO MAIN
BACK TO MAIN
Page 1
MS
SOURCE ( ESP- )
Capillary Cone Extractor RF Lens Source Block Temp.
Desolvation Ten.
Set Rdbk
3.00 60 5 0.2 150
250
2.98 -29
-7
153
248
Pressures
.
Analyser Vacuum Gas Cell
Rdbk
3.3e-5 4 .3e-3
MSI
LM Resolution HM Resolution Ion Energy Ion Energy Ramp Lens 6
Multiplier
Set Rdbk
14.0 14.0 , 2.0 * .
0.0
56
650
MS2
LM Resolution HM Resolution Collision Ion Energy Ion Energy Ramp Lens 8 Lens 9 Multiplier
'
Set Rdbk
15.0 15.0 45 2.0 0.0 40 0 650
42
40 44
BACK TO MAIN
Masslynx - Sample List
Sample List:C:\MASSLYNX\FACT-1.PROVSampleDB\05t499c.SPL Printed: FriMay 1416:17:21 1999
wm
Page 1
FileNami FileText .
1 05149906 MeOH Blank 2 05149906 MeOH Blank 3 05149906 Tox-120, RBL04199-H20Blk-2,4/19/99 4 05149906 Tox-120, RBL04199-LBIk-2,4/19/99 5 05149906 Tox-120, RBL04199-5ppb-2,4/19/99 6 05149906 Tox-120, RBL04199-1Oppb-2,4/19/99 7 05149906 Tox-120, RBL04199-25ppb-2, 4/19/99 8 05149906 Tox-120, RBL04199-50ppb-2, 4/19/99 9 05149906 Tox-120, RBL04199-100ppb-2,4/19/99 10 05149907 Tox-120, RBL04199-250ppb-2,4/19/99 11 05149907 Tox-120, RBL04199-500ppb-2, 4/19/99 12 05149907 Tox-120, RBL04199-750ppb-2,4/19/99 13 05149907 Tox-120, RBL04199-1000ppb-2,4/19/99 14 05149907 MeOH Blank 15 05149907 Tox-120 23M G2/D1:25 16 05149907 Tox-120 24M G2/D1:25 17 05149907 Tox-120 25MG2/D1-.25 18 05149907 Tox-120 26M G2/D1:25 19 05149907 Tox-120, RBL04199-250ppb-CC1,4/19/99 20 05149906 MeOH Blank 21 05149908 Tox-120 33M G3/D1:25 22 05149908 Tox-120 34M G3/01:25 23 05149908 Tox-120 35M G3/D1:25 24 05149908 Tox-120 36M G3/D1:25 25 05149908 Tox-120 37MG3/D1:25 26 05149908 Tox-120 38M G3 /0125 27 05149908 Tox-120 39M G3 /D1:25 28 05149908 Tox-120 40M G3/D125 29 05149908 Tox-120, RBL04199-250ppb-CC3,4/19/99 30 05149909 MeOH Blank 31 05149909 Tox-120 27MG2/D1:10 32 05149909 Tox-120 29MG2/01:10 33 05149909 Tox-120 30MG2/D1:10 34 05149909 Tox-120 31M G2/D1:10 35 05149909 Tox-120, RBL04199-250ppb-CC4,4/19/99 36 05149909 MeOH Blank
37 05149909 Tox-120 42M G3/D1:10
38 05149909 Tox-120 43M G3/D1:10 39 05149909 Tox-120 45M G3/D1:10 40 05149910 Tox-120 46M G3/D1:10 ' 41 05149910 Tox-120 47MG3/D1:10 42 05149910 Tox-120, RBL04199-250ppb-CC5,4/19/99 43 05149910 MeOH Blank 44 05149910 Tox-120, RBL04199-H20Blk-2,4/19/99 45 05149910 Tox-120, RBL04199-LBIk-2,4/19/99 46 05149910 Tox-120, RBL04199-5ppb-2,4/19/99 47 05149910 Tox-120, RBL04199-1Oppb-2,4/19/99 48 05149910 Tox-120, RBL04199-25ppb-2, 4/19/99 49 05149910 Tox-120, RBL04199-50ppb-2, 4/19/99 50 05149911 Tox-120, RBL04199-100ppb-2,4/19/99 51 05149911 Tox-120, RBL04199-250ppb-2,4/19/99 52 05149911 Tox-120, RBL04199-500ppb-2,4/19/99 53 05149911 Tox-120, RBL04199-750ppb-2,4/19/99 54 05149911 Tox-120, RBL04199-1000ppb-2,4/19/99
M S File inletI Botti ilume Sample T Cone A
TELMER 9min- 96
10 Analyte
TELMER 9min- 96
10 Analyte
TELMER 9min- 41
10 Analyte
TELMER 9min- 42
10 Analyte
TELMER 9min- 43
10 Standard 4.93
TELMER 9min- 44
10 Standard 9.76
' TELMER 9min- 45
10 Standard 24.8
TELMER 9min- 46
10 Standard 49.3
TELMER 9min- 47
10 Standard 97.6
TELMER 9min- 48
10 Standard 248
TELMER 9min- 49
10 Standard 493
TELMER 9min- 50
10 Standard 735
TELMER 9min- 51
10 Standard 976
TELMER 9min- 96
10 Analyte
TELMER 9min- 52
10 Analyte
TELMER 9min- 53
10 Analyte
TELMER 9min- 54
10 Analyte
TELMER 9min- 55
10 Analyte
TELMER 9min- 56
10 Analyte
TELMER 9min- 96
10 Analyte
TELMER 9min- 57
10 Analyte
TELMER 9min- 58
10 Analyte
TELMER 9min- 59
10 Analyte
TELMER 9min- 60
10 Analyte
TELMER 9min- 61
10 Analyte
TELMER 9min- 62
10 Analyte
TELMER 9min- 63
10 Analyte
TELMER 9min- 64
10 Analyte
TELMER 9min- 65
10 Analyte
TELMER 9min- 97
10 Analyte
TELMER 9mn- 66
10 Analyte
TELMER 9min- 67
10 Analyte
TELMER 9min- 68
10 Analyte
TELMER 9min- 69
10 Analyte
TELMER 9min- 70
10 Analyte
TELMER 9min- 97 TE L M E R 9min- 71
10 Analyte 10 Analyte
TELMER 9min- 72 10 Analyte
TELMER 9min- 73
10 Analyte
TELMER 9min- 74
10 Analyte
TELMER 9min- 7 5 " 10 Analyte
TELMER 9min- 76
10 Analyte
TELMER 9min- 98
10 Analyte
TELMER 9min- 41
10 Analyte
TELMER 9min- 42
10 Analyte
TELMER 9min- 43
10 Standard 4.93
TELMER 9min- 44
10 Standard 9.76
TELMER 9min- 45
10 Standard 24.8
TELMER 9min- 46
10 Standard 49.3
TELMER 9min- 47
10 Standard 97.6
TELMER 9min- 48
10 Standard 248
TELMER 9min- 49
10 Standard 493
TELMER 9min- 50
10 Standard 735
TELMER 9min- 51
10 Standard 976
Factor 1 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.0O 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000 0.000
Function i 1
MRU of 3 H u s sirs ( S O - )
Intar Channel Delay ( Secs ) i
Span ( Daltons ) t
Start Tine { Kins ) *
End Time ( Hina ) t
Repeats s
i
Channel Parent Daughter
0.03 0.00 0.00 9.00
Dwell
(Secs) Coll Energy (eV)
Cone ( V )
413.00 119.00 413.00 169.00 413.00 219.00
0.25 0.25 0.25
30 20 30
15 15 15
Function i 2
MX* of 1' Mass Fair ( ESP- J
Inter Channel Delay ( Secs ) t Span < Daltons ) i Start Tine ( Mins ) * End Time ( Kins ) * Repeats x
Channel Parent Daughter
0.03
0.00
0.00
'
9.00 ' . '
1
Dwell (Secs) Coll Energy (eV)
Cone ( V )
463.00 219.00 0.40
20
35
Function s 3
BUOI of 1 Mass Fair ( ESP- )
Inter Channel Delay ( Secs ) s 0.03
Span ( Daltons ) i
0.00
Start Tine ( Mins ) i
0.00
End Tine { Mins ) x Repeats t
9.00 1
Channel Parent Daughter Dwell
(Secs) Coll Energy
' (eV) Cone ( V
)
1 513.00 219.00 0.40
20
35
Function < 4
MEM of 1 Mass Fair ( ESP* )
Inter Channel Delay ( Secs ) i
Span ( Daltons ) t
Start Tine ( Mins ) *
End Tine ( Mins ) i
Repeats i
Channel Parent Daughter
0.03 0.00 0.00
9.00 1 Dwell
(Secs) Coll Energy (eV)
' Cone ( V )*
563.00 219.00 0.40
25
30
Function s 5
MIX of 1 Mass Pair ( ESP* )
Inter Channel Delay ( Secs ) i
Span ( Daltons ) t Start Tine ( Kins ) x End Tine ( Mins ) i Repeats t
*
Channel Parent Daughter
0.03 0.00 0.00 9.00 1
Dwell
(Secs) Coll Energy (eV)
Cone ( V }
13.00 219.00 0.40
20
40
Function s
m u m of 1 Mass Pair ( ESP- )
Inter Channel Delay ( Seca )
Span ( Daltons ) t
Start Tina ( Mins ) t
End T-i-n-e -(--M-i-n-a. ). t
Repeats t
*
Channel Parent Daughter
1 427.00 80.00
0.03 0.00
0.00
9.00 l
Dwell (Secs) Coll Energy (eV)
0.40
40
Cone ( V ) 60
" BACK TO MAIN
ft
FACT: DATA REVIEW SUMMARY FORM
BACK TO MAIN
Oste: s / i a / y i
Analyst:
V-t
FACTStudy Number ~ T S V - 1
Matrix;
Time Points
Dilutions?
Analyte 1:
Pq AA.
LOQfir this dataset / g o Re-evaluated brdifferentrange?
Analyte 4:
M SU2>
Acceptable LinearRange: ~~A)Q S T A jQ. ~--
LOQfor this dataset
"
Re-evaluatedfordifferent range? --
Analyte,5:
A
Acceptable LinearRange:
J2>
LOQfir this dataset
Re-evahatedfordifferentrange? ^ _____
Analyte 2:
j mn m
AcceptableLinearRange: -- fOO S^T'
LOQfir this dataset _________ --
Re-evaluatedfir differentrange?
Analyte 3:
-MSS.
Acceptable LinearRange: /
LOQfir this data set
/ ? > y 2 >
Re-evaluatedfor different range? --r
^ for /W V63 -/Ae LO 1% A^ybyvvwtelL /<Ta.yol. -fouw l (W, 4kc & 3 t i n t s ' * P L'io
c c f t s A T ^ O*^-iC^Y*0U-r-K..
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^ 6 x ^ /iy /ff
BACK TO MAIN
Quantity Cocapound m a r y sport TOX-120 Rat Livar Dilutions
Sasple List: B :\IACT-1.FRO\SaaplaDB\051499s Last modified: Mon May 17 14:14:20 1999
Mathod:
S:\rACT-l.PRO\MathDB\051499o
Last modified: Mon May 17 14:23:38 1999
Job Coda:
Printed:
Tua May 18 08:22:40 1999
Compound 1: P O M
Rana 1 051499061 2 051499062 3 051499063 4 051499064 5 051499065 4 051499066 7 051499067 8 051499068 9 051499069 10 051499070 11 051499071 12 051499072 13 051499073 14 051499074 15 051499075 16 051499076 17 051499077 18 051499078 19 051499079 20 051499080 21 051499081 22 051499082 23 051499083 24 051499064 25 051499065 26 051499086 27 051499087 28 051499088
29 051499089 30 051499090 31 051499091 32 051499092 33 051499093 34 051499094 35 051499095 36 051499096 37 051499097 38 051499098 39 051499099 40 051499100 41 051499101 42 051499102 43 051499103 44 051499104 45 051499105 46 051499106 47 051499107 48 051499108 49 051499109 50 051499110 51 051499111 52 051499112 53 051499113 54 051499114
a^lallat Taxt
MeOH Blank
MaOK Blank
Tox-120, RBL04199-H20Blk-2, 4/19/99
Tox-120, RBl04199-LBlk-2, 4/19/99
Tox-120, 9BL04199-5ppb-2, 4/19/99
Tox-120, 9BL04199-10ppb--2, 4/19/99
Tox-120, 9BL04199-25ppb-2, 4/19/99
Tox-120, BBL04199-50ppb-2, 4/19/99
Tox-120, BBL04199-100ppb-2, 4/19/99
Tox-120, RBL04199-250ppb-2, 4/19/99
Tox-120, BBL04199-500ppb-2, 4/19/99
Tox-120, SBL04199-750ppb-2, 4/19/99
Tox-120, B104199--1000ppb-2, 4/19/99
MeOH Blank
Tox-120 23M G2 / D1:2S
.
Tox-120 24M G2 / Dl:25 '
Tox-120 2SM G2 / 01:25
Tox-120 2K 62 / 01:25
Tox-120, RBL04199--2S0ppb-CCl, 4/19/99
MeOH Blank
.
Tox-120 33K 63 / 01:25
Tox-120 34M G3 / 01:25
Tox-120 3SM G3 / 01:25
Tox-120 36M G3 / Dl:25
-
Tox-120 37M G3 / 01:25
Tox-120 38M <53 / 01:25
Tox-120 39M G3 / 01:25
Tox-120 4QM 03 / 01:25
Tox-120, RBL04199-250ppb-CC3, 4/19/99
MeOH Blank
Tox-120 27M 62 / 01:10
Tox-120 29M G2 / Dl:10
Tox-120 3OM G2 / 01:10
Tox-120 31M G2 / 01:10
Tox-120, RBL04199--250ppb-CC4, 4/19/99
MeOH Blank
Tox-120 42M G3 / 01:10
Tox-120 43K G3 / 01:10
Tox-120 45M G3 / 01:10
Tox-120 4 M G3 / 01:10
Tox-120 4TM G3 / 01:10
Tox-120, RBlt04199-250ppb-CC5, 4/19/99
MeOH Blank
Tox-120, RBL04199--H20BU-2, 4/19/99
Tox-120, XBL04199-LBlk-2, 4/19/99
Tox-120, RBL04199-Sppb-2, 4/19/99
Tox-120, RBI04199-10ppb-2, 4/19/99
Tox-120, m04199-25ppb-2, 4/19/99
Tox-120,' RBL04199--50ppb-2, 4/19/99
Tox-120, RBL04199--100ppb-2, 4/19/99
Tox-120, 9Bl*04199-250ppb-2, 4/19/99
Tox-120, RBLD4199-500ppb-2, 4/19/99
Tox-120, HBL04199-750ppb-2, 4/19/99
Tox-120, RBL04199-1000ppb-2, 4/19/99
typ Analyta Analyte Analyta Analyta Standard Standard Standard Standard Standard Standard Standard Standard Standard Analyta Analyta Analyta Analyta Analyta C Analyta Analyta Analyta Analyta Analyta Analyta Analyta Analyta Analyta QC Analyta Analyta Analyta Analyta Analyta
0 Analyta Analyta Analyta Analyta Analyta Analyte . QC Analyta Analyta Analyta Standard Standard Standard Standard Standard Standard Standard Standard Standard
8td Cono
5.56 11.10 27.80 55.60 111.00 278.00 ' 556.00 833.00 1111.00
278.00
*
278.00
278.00
278.00
5.56 11.10 27.80 55.60 111.00 278.00 556.00 833.00 1111.00
R
5.050
5.047 5.050 5.049 5.049 5.036 5.048 5.048
5.050 . 5.048 5.049 5.049 5.049
5.048 5.050 5.050 5.050 5.056 5.049 5.048 5.049 5.049
5.050 5.048 5.049 5.050 5.050
5.048 5.049 5.048 5.049 5.048 5.049
5.052 5.048 5.049 5.051 5.050 5.049 5.050 5.050 5.049 5.048 5.051
Ara
72
132 113 175 406 801 1123 1283
368 592 283 255 464
383 601 481 . 515 503 591 .465 598 485
440 268 281 326 508
263 563 214 177 288 471
81 58 113 39 139 105 249 429 837 1175 1380
Paga 1
Response Cono. H a g s %Dev
71.686
9.88 MM
78
132.374 113.329 175.242 406.110 800.930 1122.779 1282.724
55.49 bb 41.18 bb 87.71 bb 261.22 bb 557.95 bb 799.83 bb 920.04 bbX
10 -26 -21
-6 0
-4
-17 ,
368.371 592.299 282.603 254.927 463.685
232.86 bb 401.15 bb 168.40 bb 147.60 bb 304.49 bb
10 \
382.606 601.330 480.556 515.341 502.581 590.622 464.784 597.567 485.149
243.55 bb 407.94 bb 317.17 bb 343.31 bb 333.72 bb 399.89 bb 305.32 bb 405.11 bb 320.62 bb
1 /\ 1 15 f
439.886 268.097 280.775 325.677 508.13S
286.60 bb 157.50 bb 167.02 bb 200.77 bb 337.90 bb
22 *
262.546 562.997 213.511 177.013 287.691 471.073
153.32 bb 379.13 bb 116.47 bb
89.04 bb 172.22 MM 310.04 bb
/ 12'
81.083 57.712 112.987
38.519 138.857 104.892 248.672 429.161 837.128 1175.497 1379.791
16.95 MM
0.00 MM
40.92 bbX 636
0.00 MM -100
60.37 bb
117
34.84 bb * -37
142.90 bb : . 29
278.54 bb
0
585.15 bb
5
839.45 bb
1
992.99 MMX -11
.
0M^V
.
Soup 020199f Raviawad and quantitatad by drb 5/17/99
BACK TO MAIN
Quantify Compound Sunaaxy Raport WX-120 Rat Livar Oilutiona
Saapla L U t : 8 :\r*CT-l .PRO\Sa^xlaDB\05U9o
Laat aodifiad! Mon May 17 14:14:20 1090
Mathod:
S:\nCT-l.PRO\MatliDa\OS1499a
Laat modiflod: Non May 17 14:23:3 1999
Job Coda:
Prlntad:
Tua May 1 08:22:40 1999
Coofxnmd 2: M483
Kama 1 051499061 2 051499062 3 051499063 4 051499064 5 051499065 6 051499066
S7 051499067 051499068 9 051499069 10 051499070 11 051499071 12 051499072 13 051499073 14 051499074 15 051499075 16 051499076
xa17 051499077 051499078 19 051499079 20 051499080 21 051499081 22 051499082 23 051499083
24 051499084 25 051499085 26 051499086 27 051499087 28 051499088 29 051499089 30 051499090 31 051499091 32 051499092 33 051499093 34 051499094 35 051499095 36 0S1499096 37 051499097 38 051499098 39 051499099 40 051499100 41 051499101
42 051499102 43 051499103 44 051499104 45 051499105 46 051499106 47 051499107 46 051499108 49 051499109 50 051499110 51 051499111 52 051499112 S3 051499113 54 051499114
a^lallat Taxt
MaOH Blank
MaOH Blank
Tox-120, RBL04199-H2QBlk-2, 4/19/99
TOX-120, RBL04199-LBlk-2, 4/19/99
TOX-120, RBL04199-5ppb-2, 4/19/99
TOX-120, 9BL04199--10ppb-2, 4/19/99
Tox-120, RBL04199-25ppb-2, 4/19/99
Tox-120, RBL04199-50ppb-2, 4/19/99
TOX-120, RBL04199--100ppb-2, 4/19/99
Tox-120, RBL04199--250ppb--2, 4/19/99
Tox-120, RBL04199-500ppb-2, 4/19/99
Tox-120, RBL04199-750ppb-2, 4/19/99
Tox-120, RBL04199-1000ppb-2, 4/19/99
MaOH Blank
Tox-120 23M 02 / DI:25
Tox-120 24M 02 / DI:25
Tox-120 25M G2 / Dl:25
Tox-120 26M 02 / DI:25
Tox-120, RBL04199-250ppb-CCl, 4/19/99
MaOH Blank
/Tox-120 33M 63 /Tox-120 34M 63 /Tox-120 35M G3 /Tox-120 3SM 63 /Tox-120 37M G3 /Tox-120 38M 63 /Tox-120 39H 63 /Tox-120 4OH 63
DI:25 Dl:25 DI:25 DI:25 DI:25 DI:25 DI:25 Dl:25
,
Tox-120. RBL04199-250ppb-CC3, 4/19/99
MaOH Blank
/Tox-120 27M 62 /Tox-120 29M 62 /Tox-120 SOM 62 /Tox-120 31M 62
. DI: 10 DI:10
Dl:10 DI: 10
Tox-120, RBL04199--2S0ppb-CC4, 4/19/99
MaOH Blank
Tox-120 42M G3 / DI: 10
Tox-120 43M 63 / Dl:10
Tox-120 4SM G3 / Di: 10
Tox-120 46M 63 / DI: 10
Tox-120 47M 63 / DI: 10
Tox-120, RBL04199-250ppb-CC5, 4/19/99
MaOH Blank
Tox-120, RBL04199-H20Blk-2, 4/19/99
Tox-120, RBL04199-LBlk-2, 4/19/99
Tox-120, RBL04199-5ppb-2, 4/19/99 -
Tox-120, RBL04199-l^pb-2, 4/19/99
Tox-120, RBL04199-25ppb-2, 4/19/99
TOX-120, RBL04199-50ppb-2, 4/19/99
Tox-120, RBL04199--100ppb-2, 4/19/99
Tox-120, RBL04199-250ppb-2, 4/19/99
Tox-120, RBL04199-500ppb-2, 4/19/99
Tox-120, RBL04199-750ppb-2, 4/19/99
TOX-120, RBL04199--1000ppb-2, 4/19/99
fypm Analyta Analyta Analyta Analyta Standard Standard Standard Standard Standard Standard Standard Standard Standard Analyta Analyta
Analyta Analyta
ocAnalyta
Analyta Analyta Analyta Analyta Analyta Analyta Analyta Analyta Analyta OC Analyta Analyta Analyta Analyta
ocAnalyta
Analyta Analyta Analyta Analyta Analyta Analyta OC Analyta Analyta Analyta Standard Standard Standard Standard Standard Standard Standard Standard Standard
Std Cono
5.56 11.10 27.80 55.60 111.00 278.00 ` 556.00 833.00 1111.00
278.00
5.307 5.306 ` 5.307 5.307
278.00
5.306 5.308 5.308 5.308 5.315 . 5.307 5.306 5.307
278.00
5.308 5.306 5.307 5.307
278.00
5.306 5.307 5.306 5.307 5.306
5.56 11.10 27.80 55.60 111.00 278.00 556.00 833.00 1111.00
Araa
613 637 509 473
48 49 47 44 44 35 . 44 43
1080 1367 1207 1023
66 83 50 63 55
Aaaponaa Cono. Plaga %Dav
612.731 636.927 509.037 472.806
bbZ
tabi bbZ bbl
48.048* *48.581 46.922 43.598 43.676 34.651 44.313 42.794
MMX MMX MMX MMX MMX MMX MMX MMX
1079.684 1367.145 1206.705 1022.573
bbX bbX bbX bbX
66.125 83.086 50.209 63.197 55.155
MMX bbl MMX MMX MMX
fi .
9aga 2
Coup 020199, Raviawad and quantitatad by dzb 5/17/99
BACK TO MAIN
Quantify Compound guuaary Raport TOX-120 Rat Livar Dilutions
ampia U s t i g :\rXCT-l. PRO\SaafilaDB\05149a
Last aodifiad! Mon May IT 14:14:20 1099
Mathod:
9:\mCT>l.FRO\MathD8\OS1499a
Last aodifiad: Mon May 1? 14:23:3 1999
Job Coda:
Frlntad:
Tua May 1 09:22:40 1999 '
Faya 3
Coopound 3 : MS13
Kama
aaplaliat Tact
Atd Cono
8T
Area
Reepcmee Cono, n a g e %Denr
1 051499061 MeQH Blank
Analyte
2 051499062 MaOH Blank
Analyte
3 051499063 Tox-120, RBL04199-H20Blk-2, 4/19/99
Analyte
4 051499064 Tox-120, RBL04199-LBlk-2, 4/19/99
Analyte
5 051499065 Tox-120, RBL04199--Sppb-2, 4/19/99
Standard
5.56
6 051499066 Tox-120, RBL04199-10ppb-2, 4/19/99
Standard
11.10 5.466
13
13.419 10.23 MM
-8
7 051499067 Tox-120, RBL04199-25ppb-2, 4/19/99
Standard
27.80 5.463
34
34.422 33.18 MK
19
8 051499068 TOX-120, RBL04199-50ppb--2, 4/19/99
Standard
55.60 5.464
49
48.961 49.07 MM
-12
9 051499069 Tox-120, RBL04199-100ppb-2, 4/19/99
Standard
111.00 5.464
128 127.672 135.10 bb 22
10 051499070 Tox-120, RBL04199-250ppb-2, 4/19/99
Standard
278.00 5.465
253 253.440 272.54 bb -2
11 051499071 Tox-120; RBL04199-500ppb-2, 4/19/9.9
Standard
* 556.00 5.500
467 466.900 505.83 bb -9
12 051499072 Tox-120, RBL04199-750ppb-2, 4/19/99
Standard
833.00 5.464
763 763.341 829.80 bb
0
13 051499073 Tox-120, RBL04199-1000ppb-2, 4/19/99
Standard
1111.00 5.514
764 764.046 830.57 bbX -25
14 051499074 MaOH Blank
] 15 051499075 Tox-120 23M G2 / Dl:25 16 051499076 Tox-120 24M G2 / Dl:25 '
.
17 051499077 Tox-120 2 62 / Dl:25
18 051499078 Tox-120 26M S2 / D1:2S
Analyte Analyte Analyte Analyte Analyte
5.515
79 78.528 81.39 MM
5.513 ' 103 103.445 108.62 bb
5.514
59 58.790 59.82 MM
5.514
86 86.441 90.03 bb
V>
19 051499079 Tox-120, RBL04199-250ppb-CCl, 4/19/99 QC
278.00 5.515
245 244.847 263.15 bb -5
20 051499080
21 051499081
22 051499082
23 051499083 24 051499084 25 051499085 26 051499086 27 051499087
28 051499088
MaOH Blank
Tox-120 33M G3 / D1:2S Tox-120 34M G3 / Dl:25 Tox-120 35M G3 / Dl:25 Tox-120 3EM 63 / Dl:25 Tox-120 3TM 63 / Dl:25 Tox-120 3M 63 / Dl:25 Tox-120 39M 63 / 01:25 Tox-120 40M 63 / Dl:25
Analyte Analyte Analyte Analyte Analyte Analyte Analyte Analyte Analyte
5.514 5.515 5.516 5.516 5.523 5.515 5.514 5.515
90 76 66
. 69 71 49
. 66 64
89.556 93.44 bb 76.379 79.Q4 MM 65.982 67.68 MM 69.198 . 71.19 MM 70.905 73.06 MM 48.849 48.95 MM . 66.234 67.95 MH 64.294 65.83 MM
29 051499089 Tox-120, RBL04199-250ppb-CC3, 4/19/99 QC
278.00 5.514
241 241.294 259.27 bb -7
30 051499090 MaOH Blank
Analyte
31 051499091 Tox-120 27M 62 / Dl:10
Analyte
5.514
136 135.719 143.89 bb
32 051499092 Tox-120 29M 62 / 01:10
Analyte
5.514
173 173.108 184.75 bb
33 051499093 Tox-120 30M 62 / 01:10
Analyte
5.514
116 116.489 122.87 bb
34 051499094 Tox-120 31M 62 / 01:10
Analyte
5.515
138 137.808 146.17 bb
35 051499095 Tox-120, RBI,04199-250ppb-CC4, 4/19/99 QC
278.00 5.515
275 274.745 295.83 bb
6
36 051499096 MaOH Blank
Analyte
37 051499097 Tox-120 42M 63 / 01:10
Analyte
5.514
111 110.694 116.54 bb
38 051499098 Tox-120 43M 63 / 01:10
Analyte
5.515
121 121.136 127.95 bb
39 051499099 Tox-120 4SM 63 / 01:10
Analyte
5.512
68 67.748 69.61 MM
40 051499100 Tox-120 46M 63 / 01:10
Analyte
5.514
93 93.064 97.27 bb
41 051499101 Tox-120 47M 63 / 01:10
Analyte
5.514
71 71.230 73.41 MM
42 051499102 Tox-120, RBL04199-250ppb-CC5, 4/19/99 QC
278.00 5.515
244 244.465 262.74 bb -5
43 051499103 MaOH Blank
Analyte
44 051499104 Tox-120, RBL04199-H20Blk-2, 4/19/99
Analyte
45 051499105 Tox-120, RBL04199-LBlk-2, 4/19/99
Analyte
46 051499106 Tox-120, RBL04199-5ppb-2, 4/19/99
Standard
5.56
47 051499107 Tox-120, RBL04199-10ppb-2, 4/19/99
Standard
11.10 5.516
15 14.743 11.68 MM 5
48 051499108 Tox-120, RBL04199--25ppb-2, 4/19/99
Standard
27.80 5.515
23
23.309 21.04 MM
-24
49 051499109 Tox-120,- RBI*04199-50ppb-2, 4/19/99
Standard
55.60 5.513
50 50.399 50.65 MM * -9
50 051499110 Tox-120, RBL04199-100ppb-2, 4/19/99
Standard
111.00 5.514
116 116.114 122.46 b b 3 . 10
51 051499111 Tox-120, RBL04199-250ppb-2, 4/19/99
Standard
278.00 5.516
262 262.095 282.00 bb
1
52 051499112 Tox-120, RBL04199-500ppb-2, 4/19/99
Standard
556.00 5.515
547 546.872 593.23 bb
7
S3 051499113 TOX-120, RBI04199-750ppb-2, 4/19/99
Standard
833.00 5.513
762 761.863 828.19 bb -1
54 051499114 Tox-120, RBL04199-1000ppb-2, 4/19/99
Standard
1111.00 5.516
773 772.968 840.32 MMX -24
Soup 020199, Roviwad and quantitatad by drb 3/17/99
BACK TO MAIN
Quantify Compound Sinnary Xaport TOX-120 Rat Liver Dilutions
lampi. H a t ; 8; \rACT-l. PRO\SaaplaOB\0514Sa
Last modifiad; Mon May 17 14:14:20 I M S
Mathed:
S:\IACT-l.PR0\MatliDB\051499o
Last modifiad: Mon May 17 14:23:38 19
Job Coda:
Prlntad:
Tua May IB 08:22:40 199
Compound 4: MS63
Han 1 051499061 2 051499062 3 051499063 4 051499064 5 051499065 6 051499066 7 051499067 e 051499068 9 051499069 10 051499070 11 051499071 12 051499072 13 051499073
14 051499074 15 051499075 16 051499076 17 051499077 18 051499078 19 051499079 20 0514990B0 21 051499081 22 051499082 23 051499083 24 051499084 25 051499085 25 051499086 27 051499087 20 051499088 29 051499089 30 051499090 31 051499091 32 051499092 33 051499093 34 051499094 35 051499095 36 051499096 37 051499097 38 051499098 39 051499099 40 051499100 41 051499101 42 051499102 43 051499103 44 051499104 45 051499105 46 051499106 47 051499107 48 051499108 49 051499109 50 051499110 51 051499111 52 051499112 53 051499113
54 051499114
SanplaXist tact
MaOH Blank MaOH Blank
Tox-120, RBL04199-H20Blk-2, 4/19/9
Tox-120, *BL0419-LBlk-2, 4/19/99
Tox-120, BBL04199-5ppb-2, 4/19/9
Tox-120, RBL04199-10ppb-2, 4/19/99
Tox-120, BBL04199-25ppb-2, 4/19/99
TOX-120, RBL04199-50ppb-2, 4/19/99
TOX-120, HBL04199--100ppb-2, 4/19/9 TOX-120, R8L04199-250ppb-2, 4/19/99
TOX-120, RBL04199-500ppb-2, 4/19/99
TOX-120, RBL04199-750ppb-2, 4/19/99 Tox-120, RBL04199-1000ppb-2, 4/19/99
MaOH Blank Tox-120 23M G2 / Dl:25
.
Tox-120 24M 62 / Dl:25 -
Tox-120 2SM 62 / Dl:25
Tox-120 204 G2 / 01:25
Tox-120, RBL04199-250ppb-CCl, 4/19/9
MaOH Blank
.
Tox-120 33M G3 / D1:2S
Tox-120 34M 63 / Dl:25
Tox-120 3SM G3 / D1:2S
Tox-120 304 6 3 / 0 1 : 2 5
Tox-120 37M 63 / Dl:25 Tox-120 38M 63 / D1:2S
Tox-120 39M 63 / Dl:25
Tox-120 40M 63 / Dl:25
Tox-120, RBL04199-250ppb-CC3, 4/19/9
MaOH Blank
Tox-120 27M 62 / 01:10'
Tox-120 29M 62 / Dl:10
Tox-120 30M 62 / 01:10
Tox-120 31M 62 / 01:10
Tox-120, RBL04199-250ppb-CC4, 4/19/99
MaOH Blank
Tox-120 42M 63 / 01:10
Tox-120 43M 63 / 01:10
Tox-120 4SM 63 / 01:10 TOX-120 404 63 / 01:10
Tox-120 47M 63 / 01:10
Tox-120, RBL04199-250ppb-CC5, 4/19/99
MaOH Blank
Tox-120, RBL04199-B20Blk-2, 4/19/99
Tox-120, RBIt04199-LBlk-2, 4/19/99
Tox-120, ABL04199-5ppb-2, 4/19/99
Tox-120, RBL04199-10ppb-2, 4/19/99
Tox-120, RBL04199-25ppb-2, 4/19/99
Tox-120, RBL04199-50ppb-2, 4/19/99
Tox-120', RBL04199-100ppb-2, 4/19/99
TOX-120, RBL04199--2S0ppb-2, 4/19/99
Tox-120, RBL04199-500ppb-2, 4/19/99
Tox-120, RBL04199-750ppb-2, 4/19/99
Tox-120, BBL04199-1000ppb-2, 4/19/99
Typa Analyta Analyta Analyta Analyta Standard Standard Standard Standard Standard Standard Standard Standard Standard Analyta Analyta Analyta Analyta Analyta QC Analyta Analyta Analyta Analyta Analyta Analyta Analyta Analyte Analyte
QC Analyta Analyta Analyta Analyta Analyte QC Analyta Analyta Analyte Analyte Analyta Analyta
QC Analyta Analyte Analyta Standard Standard Standard Standard Standard Standard Standard Standard Standard
8td Cono
XT
5.56 11.10 27.80 55.60 111.00 278.00 ` 556.00 833.00 1111.00
278.00
5.671 ` 5.671 5.671
278.00 278.00
5.672 5.672 5.671 5.672
278.00
5.56 11.10 27.80 55.60 111.00 278.00 556.00 833.00 1111.00
Ama
44 15 21
.
45 70 45 47
JtACpon Cono, flags
44.162 14.826 20.809
Ma Ma Ma
45.418 70.127 44.525 47.121
Ma Ma Ma Ma
Taya 4
Soup 020199, Raviavad and quantitatad by drb 5/17/99
?
!
ro
>
o
7
o
>
Soup 020X99, Raviowad and quand tatad by drb 5/17/99
BACK TO MAIN
Quantify Compound Sunnary Report TOX-120 Rat Liver Dilution
Sample List: 8:\FACT-1.PRO\SasplaDB\051499q
Last modified: Mon May 17 14:14:20 1999
Mathod:
S:\FACT-l.PRO\MethDB\051499o
Last modified: Mon May 17 14:23:38 1999
Job Coda:
Printad:
Tua May 18 08:22:40 1999
Compound 6: ZS
# Mama 1 051499061 2 051499062 3 051499063 4 051499064 5 051499065 6 051499066 7 051499067 8 051499060 9 051499069 10 051499070 11 051499071 12 051499072 13 051499073 14 051499074 15 051499075 16 051499076 17 051499077 10 051499078
19 051499079 20 051499000 21 051499081 22 051499082
23 051499083 24 051499084 25 051499085 26 051499086 27 051499087 28 051499088 29 051499089 30 051499090 31 051499091 32 051499092 33 051499093 34 051499094 35 051499095 36 051499096 37 051499097 30 051499098 39 051499099 40 051499100 41 051499101 42 051499102 43 051499103 44 051499104 45 051499105 46 051499106 47 051499107 48 051499108 49 051499109 50 051499110 51 051499111 52 051499112 53 051499113 54 051499114
8 mapilist Tuet MeOH Blank MaOH Blank Tox-120, HBL04199--H20Blk-2, 4/19/99 Tox-120, RBL04199-lBlk-2, 4/19/99 Tox-120, RBL04199-5ppb-2, 4/19/99 Tox-120, RBIi04199-10ppb-2, 4/19/99 Tox-120, RBt,04199-2Sppb-2, 4/19/99 Tox-120, BBL04199-50ppb-2, 4/19/99 Tox-120, BBL04199-100ppb-2, 4/19/99 Tox-120, RBL04199-250ppb-2, 4/19/99 Tox-120, RBL04199-500ppb-2, 4/19/99 Tox-120, BBL04199-750ppb-2, 4/19/99 Tox-120, RBL04199-1000ppb-2, 4/19/99 MeOH Blank Tox-120 23K 62 / 01:25 Tox-120 24M 62 / 01:25 . ' Tox-120 25M 62 / 01:25 Tox-120 26M 62 / 01:25 Tox-120, RBX04199-250ppb-CCl, 4/19/99
MeOH Blank Tox-120 33M 63 / 01:25 Tox-120 34M 63 / 01:25 Tox-120 3SM 63 / Dl:25 Tox-120 34M 63 / 01:25 Tox-120 37M 63 / 01:25 Tox-120 38M 63 / 01:25 Tox-120 39M 63 / 01:25 Tox-120 40M 63 / 01:25 Tox-120, RBL04199-250ppb-CC3, 4/19/99 MeOH Blank Tox-120 27M 62 / Dl:10 Tox-120 29M 62 / 01:10 Tox-120 30M 62 / 01:10 Tox-120 31M 62 / Dl:10 Tox-120, BBL04199-250ppb-CC4, 4/19/99 MeOH Blank Tox-120 42M 63 / 01:10 Tox-120 43M 63 / 01:10 TOX-120 45M 63 / 01:10 Tox-120 46M 63 / Dl:10 Tox-120 47M 63 / 01:10 Tox-120, RBL04199-250ppb-CC5, 4/19/99 MaOH Blank Tox-120, HBL04199-H2QBlk-2, 4/19/99 Tox-120, RBL04199-LBlk-2, 4/19/99 Tox-120, RBL04199-5ppb-2, 4/19/99 Tox-120, RBL04199-10ppb-2, 4/19/99 Tox-120, RBL04199--25ppb-2, 4/19/99 Tox-120, RBL04199--50ppb-2, 4/19/99 Tox-120, RBL04199--100ppb-2, 4/19/99 Tox-120, R B U M l O ^ S O p p b ^ , 4/19/99 Tox-120, RBL04199-500ppb-2, 4/19/99 Tox-120, RBL04199-750ppb-2, 4/19/99 Tox-120, RBL04199-- 1000ppb-2, 4/19/99
Typa Analyte Analyte Analyta Analyta Standard Standard Standard Standard Standard Standard Standard Standard Standard Analyta Analyta Analyta Analyta Analyta QC Analyta Analyta Analyta Analyta Analyta Analyta Analyta Analyta Analyta QC Analyta Analyta Analyta Analyta Analyta QC Analyta Analyta Analyta Analyta Analyte Analyta QC Analyte Analyta Analyta Standard Standard Standard Standard Standard Standard Standard Standard Standard
Std Cono
RT
5.036 5.035 1.00 5.036 1.00 5.036 1.00 5.033 1.00 5.036 1.00 5.034 1.00 5.035 . 1.00 5.023 1.00 5.034 1.00 5.034
1.00
5.036 5.034 * 5.035 5.035 5.035
1.00
5.034 5.036 5.036 5.036 5.042 5.035 5.034 5.035 5.035
1.00
5.037 5.034 5.035 5.036 5.036
1.00
5.034 5.035 5.034 5.035 5.034 5.035
1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00
5.038 5.034 5.035 5.037 5.036 5.035 5.036 5.036 5.035 5.034 5.037
Area
Response Cono. Flags %Dav
2407 2496 2699 2722 2446 2280 2566 2352 . 2408 2309 2302
2406.723 2496.454 2699.373 2722.382 2446.267 2280.384 2565.513 2351.564 2408.171 2308.686 2301.694
81 109
74 70 2336
81.237 109.041
74.247 69.750 2336.227
170 81 71 95 84 83
. 91 73
2253
169.517 80.550 71.387 94.601 04.019 82.882 96.582 72.744
2253.416
163 198 184 179 2464
163.472 198.362 183.888 178.796 2464.372
214 175 164 170 161 2414
213.740 174.571 163.908 169.648 160.737 2413.697
2416 2396 2542 2751 2500 2337 2586 2464 2333 2355 2457
2415.683 2395.952 2541.736 2751.209 2500.397 2336.569 2585.568 2463.607 2333.406 2355.073 2457.479
0.98 bb 1.01 bb 1.09 bb 1.10 bb 0.99 bb 0.92 bb 1.04 bb 0.95 bb 0.98 bb 0.94 bb 0.93 bb
-2
1 9 10
-1 -8
4 -5 -2 -6 -7
0.03 bb 0.04 bb 0.03 Ml 0.03 MM 0.95 bb
-97 -96 -97 -97
-5
0.07 bb 0.03 bb 0.03 MM 0.04 bb 0.03 bb 0.03 bb 0.04 bb 0.03 MM 0.91 bb
-93 -97 -97 -96 -97 -97 -96 -97
-9
0.07 bb 0.08 bb 0.07 bb 0.07 bb 1.00 bb
-93 -92 -93 -93
0
0.09 bb 0.07 bb 0.07 bb 0.07 bb 0.07 bb 0.98 bb
-91 -93 -93 -93 -93
-2
0.98 bb
-2
0.97 bb
-3
1.03 bb
3
1.12 bb
12
1.01 bb
1
0.95 bb * -5
1.05 bb ?.
5
1.00 bb * 0
0.95 bb
-5
0.95 bb
-5
1.00 MM
0
Paga 6
Soup 020199, Reviewed and quantitatad by drb 5/17/99
Quantlfy Calibrati Raport TOX-120 Hat U v w D ilu tlo n a
C a lib ra t i e n i a :\n tC T -l. FM>\CarvaSB\051488e
L aat a o d if la d : Tea May 18 08:21:03 1
P z ln tad :
Tua Miy 18 08:22:33 1888
'
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* l
p>* 1
Boup 020188, Ravlavad and quantitatad by dxb 5/17/88
Quantify Calibration Raport TOX-120 Rat Livar Dllutlona
Calibratlons R:\mcT-l.FR0\CurvaDR\051499a laat nodiriad: Ina May IR 0R:21:03 1999
Printad:
Tua May IR 0Ri22:S3 1999
'
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' PS 2
.' '
Roup 020199, Ravlawad and quantltatad by drb 5/17/99
Quantify Calibration Itaport TOX-120 Hat Llvar Dilution
Calibration: S:\TACT-l.PR0\CurvaDB\0S1499a
Last nodifiad: Tua Hay It 08:21:03 1999
Printad:
Tua May 18 08:22:53 1999
BACK TO MAIN
Soup 02019, Baviawad and quantitatad by drb 5/17/99
Ouantify Calibratimi tepect TCK-120 Rat Livar Dilutions
Calibratimi: a:\nkCT-1.9RO\CurvuD8\051499a
Laat Bodifiad: Tua May 11 08:21:03 1998
Printad:
tua May 19 08:22:83 1999
BACK TO MAIN
Paga 4
Soup 020199, Rawiaaad and quantitatad by drb 5/17/99
1
Quantify Calibration Raport TOX-120 Rat Llvar Dilutlona
Calibrition: I:\r*cr-l.rao\CurvDB\0314990
Lait aodiflad: Tua May la 08:21:03 1900
Frlntad:
Tua May la 08:22:53 1999
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* Paga 5
8oup 020199, Ravlawad and quantitatad by dzb 5/17/99
Quantify Calibrati! Raport TOX-120 Rat U v a r Dilati
Calibrati!! I:\riCT-i.PJO\CurvaDB\051499o
Laat aodifiad: Tua May la 08:21:03 1 0 M
Frintad:
Tua May lt 08:22:53 1099
BACK TO MAIN
' *
<
Method Report
Method file: Lest modified:
Compound:
Printed:
S:\rACT-l.PRO\MethDB\05H99c.mdb Mon May 17 14:23:38 19990
1: P C M
Tue May 18 08:41:45 1999
Compound Heme: Internal Ref.: Quantify Trace: Cone, of Standards:
PCM [Hone] TIC Cone. A
User RT Value:
User Peak Factor: Integrate Window Factor:
1.000000, Disabled 1.000000
2 .0 0 0
Peak Location
Retention Time (mina) : Relative Retention' Time: Time Window (mine):
4.998, enabled
0.000, Disabled 0.400
Peak Selection:
Nearest
Response
Response Type: j Response:
Esternai Areas
Calibration Curves
I Polynomial Type:
' Llneer
I Point of Origin:
Exclude
1 Fit weighting:
1/X
I Axis Transformation:
None
Concentration
Units:
Noise
Peak-to-peak amplitude:
140
Smooth
.
Smoothing: Window size (scans): Number of smooths: Smoothing method:
Disabled 3 2
Baselines
Join valley threshold: Reduce peak tailing: Raise baseline maximum:
20.00 100.00 0.00
Peak Separation
Draw vertical threshold:
Detect shoulders: Shoulder threshold:
10.00
Disabled 40.00
Response Threshold
Relative height: Absolute height: Relative area:
Absolute area:
1.50, Disabled O.OO, Disabled 2.00, Disabled
0.00, Enabled
Spty O l D l W
.`x
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Page 1
Method Report
Method file: Lest modified:
Compound:
Printed:
S:\FXCT-1 .PRO\MethDB\051499c.mdb Mon Mey 17 14:23:38 19990
2: H463
Tue Mey 18 08:41:45 1999
Compound Heme: Internal Ref.:
Quantify Trees: Cone, of Standards:
M463 [Nona] TIC Cone. A
User RF Value: User Peek rector: Integrate Window Factor:
1.000000, Disabled
0.000000 2.000
Peek Location
Retention Time (mins): Relative Retention Time: Time Window (mins):
5.408, Enabled 0.000, Disabled 0.551
Puk S.laction:
Neareat
Response
Kaapona. Typ: Kaapona.:
'
Extarnal
.
Araas . -
Calibration C o m a
Polynomial Typa:
Point of Origin: Fit Knighting: Axis Transformation:
Linear
Exclude 1/X
None
Concentration
Units:
Noise
Psak-to-paak aapiituda:
140
Smooth
Smoothing: Window size (scans): Number of smooths: Smoothing method:
- Disabled *3
2
Baselines
Join valley threshold: Reduce peek tailing: Raiae baseline maximum:
20.00
100.00 0.00
Peak Separation
Draw vertical threshold:
Detect shoulders:
,
Shoulder threshold:
10.00
Disabled 40.00
*
Aasoonsa Threshold
Relative height: absolute height* Relative area:
Absolute area:
1.50, Disabled : O.OO, Disabled
2.00, Disabled
80.00, Enabled
(AtSn/xp n T lW Offaojlj
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Pag* 2
H.thod Report
Method file! Lest aodifltd: Compound:
Printed:
S:\raCT-l.MU>\MethD8\051499c.ndb Mon May 17 14:23:3 19990
3: MS13
Tue Hey IS 0S:41:4S 1999
Compound Mame: Internal Rei.: Quantify Trace: Cone, of Standards:
M513 [None] TIC Cone. A
User XT Valu! User Peak Factor! Integrato Window factor!
1.000000, Disabled 1.000000
2 .0 0 0
Peak Location
Retention Tina (nina): Relative Retention Times Time Window (mine) :
5.538, Enabled 0.000, Disabled 0.325
Peak Selection:
Nearest
Reaponae
Reaponae Type: Reaponae:
External Areas
Calibration Curves
Polynomial Type: Point of Origin: Tit Weighting: Axis Transformation:
Linear Exclude 1/X None
Concentration
Units:
*
Noise
Peak-to-peak amplitude:
140
Smooth
.
Smoothing: Window size (scans):
Number of smooths: Smoothing method:
Disabled 3 *2
Baselines
Join valley threshold: Reduce peak tailing: Raise baseline maximum:
20.00 100.00 0.00
Peak Separation
Draw vertical threshold: Detect shoulders: Shoulder threshold: x*
10.00 Disabled
40.00
Response Threshold
Relative height: Absolute height: Relative area:
Absolute treat
1.50, Disabled 0.00, D is a b le d 2.00, Disabled
60.00, Enabled
U c v/loljf
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Page 3
Method Report
Method file: Last modified:
Compound:
Printed:
S:\FACT-1. PRO\MethD8\051499c.i Mon May 17 14:23:38 1999Q
4: MS63
Tue Kay 18 08:41:46 1999
Coapound Name: Internal Ref.:
Quantify Trace: Cone, of Standards:
User AT Value: User Peak Factor: Integrate Window rector:
Peak Location
Retention Tijne (nine): Relative Retention Tina: Tine Window (mins):
P u k S.l.ction:
'
Raisons.
Response Type: Response:
Calibration Cnrni
Polynomial Type: Point of Origin:
Fit Weighting: Axis Transformation:
Concentration
Onitii
Wbise
ioak-to-paak aaplltuda:
Smooth
Smoothing: Window size (scans): Number of smooths: Smoothing method:
Baselines
Join valley threshold:
Reduce peak tailing: Raise baseline maximum:
Peak Separation
Draw vertical threshold: Detact shoulders: Shoulder threshold: -
Response Threshold
Relative height: Absolute height:. Relative area: Absolute area:
a
m a
oeooe oooooo ooo HHN
M563 (Nona) TIC Cone. A
5.349, Enabled 0.000, Disabled 0.511 Raarast
External Areas
Linear Exclude 1/X Hone
140
Disabled 3 2
20.00 100.00 0.00
10.00 Disabled 40.00
1.50, Disabled > 0.00, Disabled
2.00, Disabled 0.00, Enabled
I
asliefa iAt OX,' M f f
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Paga 4
Method Report
Method file: haet modified:
S:\nCT-l. PRO\MethDB\OS1499c.mdb Mon May 17 14:23:3 19990
Coapound:
S: M613
Printed:
Tua May 1 08:41:46 1999
Compound Ki m : Internal Ref.: Quantify Trace: Cone, of Standardss
H613 [Nona] TIC Cone. A
SbUj) AlftH9 tAL
Usar RT Valus* Usar Pask factor: Intagrata Window Factor:
1.000000, Disabled 1.000000
2 .0 0 0
Peak Location
Ratantion Tima (mins) * Relative Retention Tima:
Tima Window (mins) *
5.853, Enabled 0.000, Disabled
0.275
Peak Selection*
Nearest
Response
Response Type* Response*
External Areas
Calibration Curses
Polynomial Type* Point of Origin* Fit Weighting: Axis Transformation:
Linear Exclude
l/X .
None
Concentration
Units:
Noise
Peak-to-peak amplitude:
140
Smooth
Smoothing: Window sire (scans): Number of smooths: Smoothing method:
Disabled 3 2
Baselines
Join valley threshold: Reduce peak tailing: Raise baseline maximum:
20.00 100.00
0.00
Peak Separation
Draw vertical threshold* Detect shoulders: Shoulder threshold: .'
10.00 Disabled 40.00
Response Threshold
Relative height: Absolute height: Relative area:
Absoluta area:
1.50, Disabled 0.00, Disabled
2.00, Disabled
80.00, Enabled
BACK TO MAIN
Pag 5
Method Report
Method file: Last Modified:
Compound:
Printed:
S:\IACT-1.PRO\MathDB\051499c.i Hon May 17 14:23:38 19990
6: ZS
Tue May 18 08:41:46 1999
Compound Hama: Internal Ref.: Quantify Trace: Cone, of Standards:
ZS [None] TIC Fixed
User RF Value:
1.000000, Disabled
User Peak Factor:
1.000000
l Integrate Window Factor: 2 .0 0 0
Peak Location
Retention Time [asina):
Relative Retention Time: Time Window (mins) :
5.034, Enabled
0.000, Disabled 0.200
Peak Selection:
Nearest
KMDOtkl.
] Response Typ.l lupanni
External Areas .
Calibration Curves
Polynonial Type: Point of Origini Fit Weighting: Axis Transformation:
Linear Exclude
1/X
Mona
Concentration
Units:
Noise
Peak-to-peak amplitude:
140 '
Smooth
I Smoothing: Window size (scans): Number of smooths: Smoothing method:
Disabled
3 2
`
Baselines
Join valley threshold: Reduce peak tailing: Raisa baseline maximum:
20.00 100.00 0.00
1 Peak Separation Draw vertical threshold: Detect shoulders: Shoulder threshold: 0
10.00 Disabled 40.00
Response Threshold
! Relative height: Absolute height: Relative area: Absolute area:
!
1.50, Disabled , 0.00, Disabled
2.00, Disabled '80.00, Enabled
I
blolM M e of/zbfa
l
BACK TO MAIN
Page 6
I
1
J ]
1
]
]
' 1
I
I
SOUP 020199: FA CT E quipm ent Log 99014
Quattron, #6414E
DATE: 05/21/99
.
ANALYST: drb
FACT Method: ETS-8-S.0 .
DESCRIPTION: Dilutions/Telmer
Injectionvolume: IO11T. Cycletime:9 min. Electrode:Z-Sprav
Mobile Phase:
fA12.0m M NH4CH3C02
/BlMeOH
TN-A-orstndrdnumber /Al 99011-6-9
B1TN-A-2540
Prepared(date/by): /Al04/28/99drb
fBl05/21/99kih
Expires:
A110/99
Storeatroomtemp? Jy| or N
Other:
fBl 10/99 .
'
Guard column: None
Partnumber: na
' Lot number:na
Analyticalcnhimn: KevstoneBetasil
Size:2 x50m m
Tvne:C18
Partnumber: 055-701-2
Lotnumber:P7L04
Sphere size:5 um Serialnumber:0481175Z
Analyticalparameters: DrvineeasfL/hrl:350 ESInebulizinggas(L/hr)l?,,, SheathgasfL/hrl0 LC Flowrate:300nT/min. Backpressure:55bar
HPLC solventgradient:9min-2
Tintefmin.l n 0.QQ_______
2)_LQQ________
314.50_______ 416.50_______
51 7.00_______
%B 400
MSL
95.0 95.0 40.0_
BACK TO MAIN
Tuning Method Report
Method:
C s\MASSLYNX\FACT-1. PR0\ACQUDB\PF0S2
Printed:
Fri May 21 16:28:16 1999
BACK TO MAIN
Page 1
MtMrn
MS MS2
SOURCE ( ESP- )
Capillary
Cone
.
Extractor
RF Lens
.
Source Block Temp.
Desolvation Temp.
Set Rdbk
3.01 40 5 0.2 150
250
3.01
-46
-8
153
254
Mit, .-.1^5
MSI
LM Resolution HM Resolution Ion Energy Ion Energy Ramp Lens 6
Multiplier
Pressures
'
Analyser Vacuum Gas Cell
Rdbk
2.4e-4 4.8e-3
.V
MS2
LM Resolution HM Resolution Collision Ion Energy Ion Energy Ran Lens 8 Lens 9 Multiplier
Set Rdbk
14.0 14.0 2-0 0.0 ' ' 53
650
Set Rdbk
15.0 15.0 45 2.0 0.0 40 0 650
43
39 43
Masslynx Sample List
Sample List: C:\MASSLYNX\FACT-1 .PRO\Sam pleDB\052199b.SPL Printed: Fri May 21 16:28:031999
BACK TO MAIN
Page 1
H ie Name
File Text
1 052199068
MeOH Blank
2 052199069
MeOH Blank
3 052199070
Tox-120, RBL04199-H20Blk-1,4 /1 9 /9 9
4 052199071
Tox-120, RBL04199-LBIk-1,4/19/99
5 052199072
Tox-120, RBL04199-5ppb-1,4 /1 9 /9 9
6 052199073
Tox-120, RBL04199-1 Oppb-1,4 /1 9 /9 9
7 052199074 ' Tox-120, RBL04199-25ppb-1,4/19/99
8 052199075
Tox-120, RBL04199-50ppb-1,4 /1 9 /9 9
9 052199076
Tox-120, RBL04199-100ppb-1,4 /1 9 /9 9
10 052199077
Tox-120, RBLQ4199-250ppb-1,4 /1 9 /9 9
11 052199078
Tox-120, RBL04199-500ppb-1,4 /1 9 /9 9
12 052199079
Tox-120, RBL04199-750ppb-1,4 /1 9 /9 9
13 052199080
Tox-120, RBLQ4199-1000ppb-1,4 /1 9 /9 9
14 052199081
MeOH Blank
15 052199082
Tox-120 4 9 M G 4 /D 1 :1 0
16 052199083
Tox-120 50M G 4 /D 1 :1 0
17 052199084
Tox-120 51M G4 / D1:10
18 052199085
Tox-120 5 2 M G 4 /D 1 :1 0
19 052199086
Tox-120, RBL04199-250ppb-CC1,4 /1 9 /9 9
20 05219987
MeOH Blank
21 052199088
Tox-120 5 3 M G 4 /D 1 :1 0
22 052199089
Tox-120 5 4 M G 4 /D 1 :1 0
23 052199090
Tox-120 5 5 M G 4 /D 1 :1 0
24 052199091
Tox-120 56M G 4 /D 1 :1 0
25 052199092
Tox-120, RBL04199-250ppb-CC3,4/19/99
26 052199093
MeOH Blank
'
27 052199094
Tox-120 6 5 M G 5 /D 1 :5 0 0
28 052199095
Tox-120 6 6 M G 5 /D 1 :5 0 0
29 052199096
Tox-120 6 7 M G 5 /D 1 :5 0 0
30 052199097
Tox-120 68M G 5 /D 1 :5 0 0
31 052199098
Tox-120, RBL04199-250ppb-CC11 ,4 /1 9 /9 9
32 052199099
MeOH Blank
33 052199100 Tox-120 69M G 5 /D 1 :5 0 0
34 052199101
Tox-120 7 0 M G 5 /D 1 :5 0 0
35 052199102 36 052199103
Tox-120 71M G5 / D1:500 Tox-120 72M G5 / D1:500
37 052199104
TOX-120, RBL04199-250ppb-CC1, 4/19/99
38 052199105
MeOH Blank
39 052199106
Tox-120 7 3 M G 5 /D 1 :2 5 0
40 052199107
Tox-120 7 4 M G 5 /D 1 2 5 0
41 052199108
Tox-120 7 5 M G 5 /D 1 :2 5 0
42 052199109
Tox-120, RBL04199-250ppb-CC11 ,4 /1 9 /9 9
43 052199110
MeOH Blank
44 052199111
Tox-120 7 7 M G 5 /D 1 5 5 0
45 05219912
Tox-120 78M G 5/D 1 50
46 052199113
Tox-120 7 9 M G 5 /D 1 :2 5 0
47 052199114
Tox-120, RBL04199-250ppb-CC1,4 /1 9 /9 9
48 052199115
MeOH Blank
49 052199116
Tox-120, RBL04199-H2OBIk-1,4/19/99
50 052199117
Tox-120, RBL04199-LBlk-1,4/19/99
51 052199118
Tox-120, RBL04199-5ppb-1,4 /1 9 /9 9
52 052199119
Tox-120, RBL04199-10ppb-1,4/19/99
53 052199120
Tox-120, RBL04199-25ppb-1,4/19/99
54 052199121
Tox-120, RBL04199-50ppb-1,4 /1 9 /9 9
55 052199122
Tox-120, RBL04199-100ppb-1,4/19/99
56 052199123
Tox-120, RBL04199-250ppb-1, 4/19/99
57 052199124
Tox-120, RBL04199-500ppb-1, 4/19/99
58 052199125
Tok-120, RBL04199-750ppb-1,4 /1 9 /9 9
59 052199126
Tox-120, RBL04199-1000ppb-1,4/19/99
. .
M S H ie
T E -P F 2 T E U -P F 2 TELI-PF2 T E L I-P F 2 T E U -P F 2 TELI-PF2 T E U -P F 2 T E U -P F 2 T E U -P F 2 . TEU-PF2 T E U -P F 2 T E U -P F 2 T E -P F 2 T E U -P F 2 T E -P F 2 T E U -P F 2 T E U -P F 2 TELI-PF2 T E U -P F 2 T E U -P F 2 T E U -P F 2 T E U -P F 2 T E U -P F 2 T E U -P F 2 T E U -P F 2 T E U -P F 2 T E U -P F 2 T E U -P F 2 T E U -P F 2 TELI-PF2 TELI-PF2 TELI-PF2 T E U -P F 2 T E U -P F 2 TELI-PF2 T E U -P F 2 T E U -P F 2 T E U -P F 2 T E -P F 2 T E U -P F 2 T E U -P F 2 T E U -P F 2 T E U -P F 2 T E U -P F 2 T E U -P F 2 T E U -P F 2 T E U -P F 2 T E -P F 2 T E U -P F 2 T E U -P F 2 T E U -P F 2 T E U -P F 2 T E U -P F 2 T E U -P F 2 T E U -P F 2 T E U -P F 2 . TEU-PF2 TELI-PF2 T E U -P F 2
Inlet H ie Bottle lume Sam ple T
9min-2 9m in-2 9m in-2 9min-2 9min-2 9min-2 9min-2 9min-2 9min-2 9min-2 9min-2 9mln-2 9mIn-2 9min-2 9min-2 9min-2 9min-2 9min-2 9min-2 9min-2 9min-2 9mln-2 9min-2 9min-2 9min-2 9min-2 9m in-2 9min-2 9m in-2 9m in-2 9m in-2 9m in-2 9m in-2 9m in-2 9m In-2 9m ln-2 9m ln-2 9m in-2 9m in-2 9min-2 9m in-2 9m in-2 9mln-2 9mln-2 9min-2 9min-2 9mln-2 9min-2 9mln-2 9min-2 9mln-2 9m!n-2 9min-2 9m ln-2 9min-2 9mIn-2 9min-2 9min-2 9min-2
91 91 79 80 81 82 83 84 85 86 87 88 89 92 57 58 59 60 90 96 61 62 63 64 99 92 65 66 67 68 100 93 - 69 70 71 72 90 94 73 74 75 100 95 76 77 78 90 96 79 80 81 82 83 84 85 86 87 88 89
.
10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 Standard 10 Standard 10 Standard 10 Standard 10 Standard 10 Standard 10 Standard 10 Standard 10 Standard 10 Analyte
10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 QC
10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 Analyte
10 Analyte 10 Analyte
10 Analyte
10 QC
10 Analyte
10 Analyte
to Analyte
10 Analyte 10 Analyte
10 QC 10 Analyte 10 Analyte
10 Analyte 10 Analyte 10 Analyte
10 Analyte 10 Analyte
10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 Analyte 10 Standar 10 Standar 10 Standai 10 Standai 10 Stanta'
10 Stanta 10 Stanta 10 Stanta 10 Stanta
Searmisa <*thod sport
Msthodl
ct\ O M B L r X X \ T C T - l .WOUCQOB\TKtI-Pfa
U i t Modifiedz Fri May 21 15:13:53 199$
Printedt
Fri May 21 16:28*25 1999
l o ln at Stliy ( ina ) s
0.00
Fuseti,oa < 1 MRM of a Masi Faira ( ICT- 1
Zntar Osannai Dalay ( Sacs ) t Spasi ( Daltona ) t
Start Tla* ( Miai ) s
End Tina ( Mina ) t . Rapaata s
Osannai Parant Daughter
0.03 0.00 0.00 9.00 1
(S.ca) Coll Energy (V)
Cosse ( V )
1
399.00 99.00
0.25
2 399.00 130.00 0.25
45 45
60 60
HH? HH
mastics i 2 m et 2 K u i valsa ( Uf- )
Zntar Osannai Dalay ( Seca ) i 0.03
Span ( Daltona ) i
0.00
Start Tino ( Mina J s
0.00
End Tina ( Mina ) s
9.00
Rapaata s
1
Osannai Parant Daughter
(S.cc) Coll Energy (V)
. Cono ( V )
1 399.00 99.00 0.25 2 299.00 130.0^1 0.25
45 45
60 60
Funetioa i 3 MRM of 1 Masa Fair ( ESP- )
Zntar Osannai Dalay ( Sacs ) t 0.03
Span ( Daltona J i
0.00
Start Tina ( Mina ) :
0.00
End Tina < Mina ) s
9.00
Rapaata *
1
Osannai Parant Daughter Duali (Sacs) Coll Energy {.V)
1
427.00 80.00
0.25
40
60
U8O
(V )
BACK TO MAIN '
FACT: DATA REVIEW SUMMARY FORM
BACK TO MAIN
Dte:
5 ~ M I S S ______ _
n c jHu.173u.no i<toto' m
ii/tt/n Pi x
sssssssssssssaatcessssasssSsssiiBSSSsaBSgscsKGsccss
illillllli
aas sasa saaaa gassa
*SS U S VN S N N N S S
333 233 2223 22 m i m mil
i- iIPI*PIp fl ; ; ( i t-
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s Sgi ,
! if!! f
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lllllit t b m ****** ***** *****lllflftff****
kCSwSt^h uwihMI hMw tmj 3m
si ibe m m ebhiooooooopooonQaOnu
oe
oo
` UUM*JK
; mm
sSSSSses
ssSs SasS s l i s t S53HS 2 . 2
lim 3SS
mm
gSmSSS
MfItO_ HI AiIHHUHMM
m m m m i' i
m s s m sm s' sssss s s
m0 m* >tS 3 .
gssssm
l*- HN
? c
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7 H O
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kf
I l t i l i tm *4 i mnnmtn*fmin4 n*mn on>vthl Il I I I I I i
fi aaaaaaaaaaa aaaaa aaaaa
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a aaaaaaaaaaa
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iuitaninnin<fniA
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riHHHHHHOOOH
4 H4H*4HHrlHHIO
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fi
5n 8m
1!
a4 s8 a19sn
3835
ss
5S6 RKf#lMRn
sm
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fi* *>
fsftm i AMA tfAiAsAsOs IIsINn fi A A W V A A f i A H H N SfififiH H H H S
V fifififiK S N S N fi **>***** H N '.A A A H H H H S
H**.HN.S-0SN <f
0s0a0s0s&i se seseseJ1
Sofia? asas? fissa? asas? sas? sas - - - *
g o Sof i oi nemo
HHHHC1 HHHN HHHN 3 JA H N lA H N A I'H
5*i i*i* **SiS5** > V.X vfi
4*, *9s s * k s \ ~ *-S-Nf-i -N-> *
t 33333 33333 88383 38883 3383 8883 33000000000
^-Bxl.l.l..i.l a l_l l i g xcsga sal ^dsnsnsnsnl ^dswsgwsl ^gsisr-arsr*l ^qsrs~srl-x qrsrapg*l ^gl s i l l l l i g l a
<i M l l<O ONONONNO (OiONNq ONNOnHBOiiO NOrOONnH NONOnOn(O iOrHOHNOrOiONOriHnOiOlNO MBNOnHnBO(#iOnnO iH i0B0n0nn0 n* n4 N0n0N0t0i0nN0 n0 <0 i0n0N0n
H H H H H H H H H H H h H H H H H rH H H H H H H H H H H H H H H H H H riH H H H H H H H H H H H W H
lUUUUUnUUUUUMUUIUUiUUI
S S N n *<n*<r,->(rt>*# *>0H<1',* in,*l'l*A e H N n * in,*r*M A e H N n * ,n'r'"*>eH,1n* n<tr'"akO,4NnviA* ---- a o o o o o a a a o o o o o a a a o a v o AAAAAAAAAAAAAAAAAAAAA A A A A A A A A A A A A A A A A A A A A f i . . ... . ...................................................H HH H HH H rlH H riH H HH H HH H HH rlHH H #'................................................................................................................................
iN N N N N r iN iin N n r in c ir iN N N n n n N N n N N N N N N n iin n N r ir if in n f t r in n n n N r ir ir ir iN N t ir in n r in foifoifiafiofifoifoiifolfiefienonfoifoinonofiAe eAeinofiefionno Mo onofiionefiioneifloinofieinoino foiinoieAofionfeime eineinafoinoifolfiainoine ionoinoinofiomafoiMomo ein.
i> i M h A O H N n fiiii < ih fiA e H n n tn h fiA O H N n in i*rfiA 4 iin n n N riN n n n n fii>in n n n <<f < n n fifiM in fifi
en oaoifli ouaacic*e*4 by ral printfid by kjh 05/34/#
Machad Report
Method file* Laat nodifled* Compoun d *
Printed*
'
Ci\MA36LYNX\PACT-l.PRO\MethDS\052199b.ndb ' Moa Kay 24 07*99*4 1999
1 f n t a
Mon Kay 24 00*14*21 1999
_____________ :____ S k u o
Incarnai Ref.t Quantify Traca* Cooa. of Standards*
Osar RP Valuat Usar Peek Pactar* Zntagrae# Window Pactari
{Mona] 399.00 > 99.00 Cene. A
1.000000, Disabled 1.000000 2.000
Rotantioa Tino (nina) t Relativa Rat antion Tina* ' Tina Window (nina) t
Paak Selection*
4.140, Enabled 0.000, Oiaablad 0.595
Maaraat
| Response Typo* Response
Xxtarnal Areas
1 Polynomial Type* j Point of Origin* ) Pit Weighting* J Axis Trena fonati* t
U nita*
Linear Exclude 1/X Nona
Peak-to-poak amplitude*
tooothing*
rJ Window size (acane)* Nunbar of aaKwtha* 1 Snoothing aathod*
11
Disabled 3 2
i Join valley thraohold*
1
Reduce peak tailing* Raise baseline a u d n a u
Draw vertical threshold* Detect shoulders* ! Shoulder threshold*
! 1
Relative height* Absolute height*
`
Relative area* ] Absolute area*
20.00 100.00 0.00 '
10.00 Disebled 40.00
1.50, Disabled 0.00, Disabled 2.00, Disabled 60.00, enabled
JI
3
j
!
i
!.*
j
j
BACK TO MAIN
. . l A C o s J x i h l ________
Mathod Aeport
Itethod filai u t and! fledi Ce^ouadi
Priatadi
'
Ci \KASSLYNX\FACT-1 .PR0\MothDS\0521**b.adb Hon lUy 24 07iS9i40 1 2t ZS
Man K*y 24 Olitili Iti*
Co^euad Nu m i Internai M f . t Quantify Tracci Cane, et Standardi
Usar XP Valuat Usar Peak Faeton Integrata M l o d w Faeton
ZS [Nona) TIC Fixcd
1.000000, Diaablad 1.000000 2.000
Hatantino Time (mina )i Relativa Ratantico Tiaar Tina Niadow (aina) i
PaaJc Salactient
5.120, Aiablad 0.000, Diaablad
0.420
Raaponaa Typai Ramponaci
eternai Araaa
Polynaaial Typai Poiat of Origini Fit Heightingi A*i Tranafoatieni
Unitai
Paak-to-paak aaplitudei
Linear bcluda X/Z Mona
H4
flnoothingi Hiadoa alza (aeansji Munbar of a-- oothai Sttoothing wathodi
Diaablad
3 2
Join volley thraaboldi Raduea paak tailingt Raiaa baaaliaa atxiaai
20.00 100.00
0.00 *
Draa vartical tbraaholdt Dataci shouldarat Shouldar thraaboldi
10.00 Diaablad 40.00
Relativa heightt Abaoluta hoighti Relativa arcai Abaoluta arcai
1.50, Diaablad 0.00, Diaablad 2.00, Diaablad
0.00. Kiablad .
5oLy? J t e Q L I
I C O s /m / H
BACK TO MAIN
ffl* 2
fluatify Calibratimi Mapatt TOX120-O9 dilutieoa
calibratimi! Ci\iaJLYjce\riCT-l.Mo\cur*ba\0S2i m
Laat modidadi Mia May 24 MiMidl IBM
Priacadi
Naa May 24 OPtiaiSB UBB
BACK TO MAIN
a 1
Bau 0201111 guaatitatad by ami primaad by kjb 09/24/Bf
Quutlfy O O U n t i M Mport TOOU20-OJ dilvtlotta
'
Calibratimi Ct\nsiUr>ac\ncr-l.Mo\Curraos\0S31ttt
lAt aodifidi Mas Itay 34 0Si04t4S ll
Printadi
M m Hqr 34 MtXOiSl 1 M
, *
BACK TO MAIN
* 3 *
.
Soup 0301 i Quutltatod by aad priotad ty kb 03/24/J
BACK TO MAIN
AM ELIA062498: FACT E quipm ent Log 99016 Quattro II,#6448E
PAGE
DATE: 05/25/99
ANALYST: hoi,sah
STUDY NUMBER Tox-120,Tox-026 MATRIX: ratliver,monkey sera DESCRIPTION: dils.
FACT Method: cts-M-S.O_____
Injectionvolumc,10uL
Cycletime: 9min. Electrode:Z-Spray_____
MobUe Phase:
(A)2.0mM NH4CH3CQ2
(B)McOH__________ .
TN-A- orstndrdnumber (A) 99012-10-7______
(B)TN-A-2540
Prepared(date/by): Expires:
(A)5/21/99ias (A)11/99
.(B)5/18/99 dtb (B) 11/99
Storeatroomtemp? Q or N
Other
Guard column: None
Partnumber na
Lot number:na
Analyticalcolumn:keystonebetasil_________ ___________ .________
Size:2x 50mm____________ Type:c!8_________ Spheresize:5um
Partnumber 055-701-2
Lotnumber P7J20 Serialnumber 038126D
Analyticalparameters: Dryinggas(L/hr):300 ESInebulizinggas(L/hr)15 LC Flowrate:300uL/min. Backpressure:70bar
Sheathgas(L/hr)0
HPLC solventgradient:9min-2
Time (min.) 1) 0.00 2) 1.0 3 4.5 5) 6.5
LZ____ _
%B 40 40
95 95 40
& - S y f e r h - i ____________ __________________________
Tuning Method Report
Method:
C :\MASSLYNX\RATS2.PRO\ACQUDB\PFOS
Printed:
Tue May 25 15:22:53 1999
BACK TO MAIN
' Page 1
MS
SOURCE ( ESP- )
Capillary
Cone
Extractor
.
RF Lens
Source Block Temp.
Desolvation Temp.
Set Rdbk
3.00 15 5 0.2
150 250
2.98 -15 -5
150 251
Pressures
Analyser Vacuum ,
Gas Cell
Rdbk
1.6e-4 4.5e-3
MSI
LM Resolution HM Resolution Ion Energy . Ion Energy.Ramp Lens 6 Multiplier
Set Rdbk
15.0 15.0 2.0 * o.o 57 650
MS2
LM Resolution
HM Resolution
Collision
Ion Energy
Ion Energy Ramp
Lens 8
Lens 9
.
Multiplier
Set Rdbk
15.0 15.0 30 2.0 0.0 40 0 650
46
40 48
t U ____________________________________ -
M asslynx Sam ple L is t
Sample List: C:\M ASSLYNX\rats2.PRO\Sam pleDB\052599a.SPL Printed: T ue May 25 15:16:14 1999
..
_
BACK TO MAIN
_____________ __________________
Page 1
F ile Name
1 052599001 2 052599002 3 052599003 4 052599004 5 052599005 6 052599006 7 052599007 8 052599008 9 052599009 1 0 052599010 1 1 052599011 1 2 052599012 13 052599013 14 052599014 15 052599015 16 052599016 17 052599017 18 052599018 19 052599019 2 0 052599020 2 1 052599021 2 2 052599022 23 052599023 24 052599024 25 052599025 26 052599026 27 052599027 28 052599028 29 052599029 30 052599030 31 052599031 32 052599032 33 052599033 34 052599034 35 052599035 36 052599036 37 052599037 38 052599038 39 052599039 40 052599040 41 052599041 42 052599042 43 052599043 44 052599044 45 052599045 46 052599046 ` 47 052599047 48 052599048 49 052599049 50 052599050 51 052599051 52 052599052 53 052599053 54 052599054 55 052599055 56 052599056 57 052599057 58 052599058 59 052599059 60 052599060
File Text
methanol blank
methanol blank
tox120, rbl04199-wblk-1
tox120, rbl04199-lblk-1
tox120, rbl04199-5ppb-1
tox120, rbl04199-10ppb-1
tox120, rbl04199-25ppb-1
tox120, rbl04199-50ppb-1
tox120, rbl04199-100ppb-1
tox120, rbl04199-250ppb-1
tox120, rbl04199-500ppb-1
tox120, rbl04199-750ppb-1
tox120, rbl04199-1000ppb-1
methanol blank
tox120, rtl, 3M -g1-250 ppb-ms, 4/19/99
tox120, rtl, 3M -g1-250 ppb-msd, 4/19/99
tox120, rtl, 110F-g1-250 ppb-msd. 2/22/99 tox120, rtl, 110F-g1-250 ppb-msd, 2/22/99
tox120, rtl, 106F-g1-250 ppb-ms, 2/22/99
tox120, rtl, 106F-g1-250 ppb-msd, 2/22/99
tox120, rbl04199-250ppb-cc-4
methanol blank
tox120, rtl, 8 1M -g 6 ,4/19/99, d1000
tox120, rtl, 8 2 M -g 6 ,4/19/99, d1000
tox120, rtl, 8 3M -g 6 ,4/19/99, d1000
tox120, rtl, 8 4 M -g 6 ,4/19/99, d1000
tox120, rtl, 8 5M -g 6 ,4/19/99, d1000
tox120, rbl04199-250ppb-cc-1 '
methanol blank
tox120, rtl, 8 6M -g 6 ,4/19/99, d1000
tox120, rtl, 8 7 M -g 6 ,4/19/99, d1000
tox120. rU, 8 8M -g 6 ,4/19/99, d1000
tox120, rtl, 8 9M -g 6 ,4/19/99, d1000
tox120, rtl, 9 0M -g 6 ,4/19/99, d1000
tox120, rbl04199-250ppb-cc-6
methanol blank
tox120, rtl, 9 1M -g 6 ,4/19/99, d1000
tox120, rtl, 9 3M -g 6 ,4/19/99, d1000 tox120, rtl, 9 4 M -g 6 ,4/19/99, d1000
tox120, rtl, 9 5M -g 6 ,4/19/99, d1000
tox120, rbl04199-250ppb-cc-3
methanol blank
tox120, rtl, 17M -g 2,4/19/99, d25
tox120, rtl, 18M -g 2,4/19/99, d25
tox120, rtl, 1 9M -g 2,4/19/99, d25 tox120, rtl, 2 0 M -g 2 ,4/19/99, d25
tox120, rtl, 2 1 M -g 2 ,4/19/99, d25
tox120, rtl, 2 2M -g 2,4/19/99, d25
tox120, rbl04199-250ppb-cc-11
methanol blank
tox120, rtl, 1 7M -g 2,4/19/99
tox120, rtl, 18M -g 2,4/19/99
tox120, rtl, 19M -g 2,4/19/99
tox120, rtl, 2 0 M -g 2 ,4/19/99
tox120, rtl, 2 1M -g 2 ,4/19/99
tox120, rtl, 2 2M -g 2 ,4/19/99
tox120, rbl04199-250ppb-cc-2
methanol blank
tox120, rtl, 4 1 M -g 3 ,4/19/99
tox120, rbl04199-wblk-1
MS File
Inlet File
9M IN-PFO S-PO AA-513-613 9mln-2 9M IN-PFO S-PO AA-513-613 9min-2
9M IN -PFO S-PO A A-513-613 9min-2
9M IN-PFO S-PO AA-513-613 9mln-2 9M IN-PFO S-PO AA-513-613 9min-2 9M IN-PFO S-PO AA-513-613 9min-2 9M IN -PFO S-PO A A-513-613 9mln-2 9M IN-PFO S-PO AA-513-613 9mln-2 9M IN-PFO S-PO AA-513-613 9min-2 9M IN-PFO S-PO AA-513-613 9min-2
9M1N-PFOS-POAA-513-613 9min-2
. 9M IN-PFO S-PO AA-513-613 9min-2
9M IN-PFO S-PO AA-513-613 9min-2
9M1N-PFOS-POAA-513-613 9mln-2
9M IN-PFO S-PO AA-513-613 9min-2
9M IN-PFO S-PO AA-513-613 9min-2
9M IN-PFO S-PO AA-513-613 9min-2
. 9M IN-PFO S-PO AA-513-613 9min-2
9M IN-PFO S-PO AA-513-613 9mln-2 9M IN-PFO S-PO AA-513-613 9min-2 ' 9M IN-PFO S-PO AA-513-613 9min-2 9M IN-PFO S-PO AA-513-613 9mln-2
9M IN-PFO S-PO AA-513-613 9min-2
9M IN-PFO S-PO AA-513-613 9mln-2
9M IN-PFO S-PO AA-513-613 9mln-2
9M IN-PFO S-PO AA-513-613 9min-2
9M IN-PFOS-POAA-513-613 9min-2 9M IN-PFO S-PO AA-513-613 9min-2
9M IN-PFO S-PO AA-513-613 9mIn-2
9M IN-PFO S-PO AA-513-613 9min-2
9M IN-PFO S-PO AA-513-613 9min-2
9M IN-PFO S-PO AA-513-613 9min-2
9M IN-PFOS-POAA-513-613 9min-2
9M IN-PFO S-PO AA-513-613 9mfn-2
9M IN -PFO S-PO A A-513-613 9min-2
9M IN-PFO S-PO AA-513-613 9min-2
9M IN -P F O S -P O M -513-613 9min-2
9M IN-PFO S-PO AA-513-613 9min-2 9M IN-PFO S-PO AA-513-613 9m in-2
9M IN-PFOS-POAA-513-613 9min-2
9M IN-PFOS-POAA-513-613 9min-2
9M IN-PFO S-PO AA-513-613 9mln-2
9M IN-PFO S-PO AA-513-613 9mln-2
` 9M IN-PFO S-PO AA-513-613 9min-2
9M IN-PFO S-PO AA-513-613 9mln-2
9M IN-PFO S-PO AA-513-613 9mln-2
9M IN-PFOS-POAA-513-613 9min-2
9M IN-PFOS-POAA-513-613 9min-2
9M IN-PFOS-POAA-513-613 9mIn-2 9M IN -PFO S-PO A A-5 3-613 9mln-2
9M IN-PFOS-POAA-513-613 9min-2
9M IN-PFOS-POAA-513-613 9M IN-PFOS-POAA-513-613 9M IN-PFOS-POAA-513-613 9M IN-PFOS-POAA-513-613
9m in-2 9m in-2 9m in-2 9m in-2
9M IN-PFOS-POAA-513-613 9mln-2
9M IN-PFOS-POAA-513-613 9mln-2
9M IN-PFOS-POAA-513-613 9min-2
9M IN-PFOS-POAA-513-613 9mln-2
9M IN-PFOS-POAA-513-613 9min-2
BACK TO MAIN
b K Sfzs fai__________ ________________ ___________ :--- =-----:----- --------------------------
Masslynx -Sample List
'
.
Page 2
Sample ListC:\MASSLYNX\rats2.PRO\SampleDB\052599a.SPL Printed:Tue May 2515:16:141999
-
File Name 61 052599061 62 052599062 63 052599063 64 052599064 65 052599065 66 052599066 67 052599067 68 052599068 69 052599069 70 052599070
FileText lox120,rb!04199-lblk-1 tox120,rbl04199-5ppb-1 tox120, rbl04199-10ppb-1 tox120, rbl04199-25ppb-1 taxi20, ibl04199-50ppb-1 tox120, rbl04199-100ppb-1 tox120, rbl04199-250ppb-1 10x120, rbl04199-500ppb-1 tox120, rbl04199-750ppb-1 tox120, rb!04199-1000ppb-1
MS File 9MIN-PFOS-POAA-513-613 9MIN-PFOS-POAA-513-613 9MIN-PFOS-POAA-513-613 9MIN-PFOS-POAA-513-613 9MIN-PFOS-POAA-513-613 9MIN-PFOS-POAA-513-613 9MIN-PFOS-POAA-513-613 9MIN-PFOS-POAA-513-613 9MIN-PFOS-POAA-513-613 9MIN-PFOS-POAA-513-613
InletFile 9mirv2 9min-2 9mn-2 9min-2 9min-2 9min-2 9min-2 9min-2 9min-2 9min-2
f e k l S ( z s ( < t e ______________________________________
canning Method Hapert
Method:
C:\MRSSUrMC\MIS2.PSO\RCODDB\3MIM-PIOS-PQM-S13-S13
Laat Modified: Tue May 23 10:33:43 1333
'
Printed:
Tue May 23 13:13:2 1333
- -
loivont Delay { mini ) :
0.00
function : 1 m m of 1 Maaa Pair ( UP - )
Intar Channel Delay { Seca ) t 0.03
Span- ( Daltona ) :
0.00
Start Tine ( Mina ) :
0.00
End Tine ( Mina ) :
9.00
Repaata :
1
Channel Parent Daughter Dwell Sees) Coll Energy (eV)
Cone ( V )
1 413.00 169.00 0.30
30
15
function : 2 MRM of 1 Maas Pair ( ESP- 1
Inter Channel Delay ( Secs ) : 0.03
Span ( Daltona ) :
0.00
Start Tina ( Mina 4 :
0.00
.
End Tine ('Mina ) :
9.00
Repeats :
1
Channel Parent Daughter Dwell (Secs) Coll Energy (.V)
Cone C V )
1 313.00 139.00 0.20
30
15
function : 3 MRM of 1 Maaa Pair ( ESP- )
Inter Channel Delay ( Secs ) : 0.03
Span { Daltona ) t
0.00
Start Tine ( Hina ) *
0.00
End Tine ( Hina ) 3
9.00
Repeata :
1
Channel Parent Daughter Dwell (Sacs) Coll Energy (eV)
Cone ( V )
1 613.00 133.00 0.20
30
15
function : 4 MRM of 1 Maaa Pair ( UP- )
Inter Channel Delay { Seca ) t 0.03
Span ( Daltona ) :
0.00
Start Tine ( Mina ) z
0.00
End Tine ( Hina ) :
9.00
Repeat! z
1
Channel Parent Daughter Dwell (Seca) Coll Energy (eV)
Cone (*V )
1 499.00 99.00 0.3Q
45
60
function : 3 MRM of 1 Maaa Pair ( UP- )
Inter Channel Delay ( Seca ) : 0.03
Span ( Deltona ) :
0.00
Start Tina ( Mina ) :
0.00
End Tlan ( Mina I :
3.00
Repeata :
>1
Channel Parent Daughter Dwell (Seca) Coll Energy (eV)
Cone ( V )
1 427.00 0.00 0.30
40
30
BACK TO MAIN
'_____________________________ ' -- .
> Paga i -.
. .
FACT: DATA REVIEW SUMMARY FORM
BACK TO MAIN
Date:
Analyst:
FACT Study Number
M atrix Time Points Dilutions?
,-
<*5
Analvte 1:
TW i f P t m M - S 7 S -
LOQ for dus data s e t
S ~ lS /y
Re-evaluated fcr different range?
4*/'
*
Analyte 4:
Acceptable Linear Range:
LOQ for this data set:
-O V /S '/
Re-evaluated for different range?
.
Analyte 2: Acceptable Linear Range:
-S ~~
LOQ for Ulis data s e t
^9--
Re-evaluated for different range?
Analyte 3:
Sh df?
Acceptable Linear Range: S~* f & ~~
LOQ for this data s e t
Re-evaluated for different range?
BACK TO MAIN
hb-{
__________ ______________________________________
Quantify Ceopeund luaaary tepori toxl20, rtl
_
Saapla Li.tl C:\MMSUNC\rata2.mo\Caaplara\0S2S99a
Laat oodlfiad: Had May 2 07:17:2 199
Mathod:
C:\MMKn0C\rats2.FIIO\MathDB\052599a
Laat aadlflad: ad May 2C 07:19:17 199
Job Coda:
Prlntad:
Wad May 26 09:20:32 199
'
_________________ ;___________________ -,.
Paga 1
.'
Compound 1: POH
f Maaa 1 052599001 2 052599002 3 052599003 4 052599004 5 052599005 6 052599006 7 052599007 8 052599008
9 052599009 10 052599010
11 052599011 12 052599012 13 052599013 14 052599014 15 052599015 16 052599016 17 052599017
18 052599018 19 052599019 20 052599020 21 052599021 22 052599022 23 052599023 24 052599024
25 052599025 26 052599026 27 052599027 28 052599028 29 052599029
30 052599030 31 052599031 32 052599032
33 052S99033 34 052599034 35 052599035
36 052599036 37 052599037
38 052599038 39 052599039 40 052599040
41 052599041 42 052599042 43 052599043
44 052599044 45 052599045 46 052S99046 47 052599047 48 052599048 49 052599049 50 052599050
51 052599051 52 052599052 53 052599053 '54 052599054 55 052S99055 56 052599056
57 052599057.
58 052599058 59 052599059 60 052599060
61 052599061 62 052599062 63 052599063 64 052599064 65 052599065 66 052599066 67 052599067 68 052599068 69 052599069 70 052599070
Sa^laliat Tut
*ypa
aathanol blank
Analyta
Rathanoi blank
Analyta
toxl20, rbl04199-wblk-l
Analyta
toxi20 rbl04199-lblk-l
Analyta
toxl20, rbl04199-5ppb-l
Standard
toxl20, rbl04199-10ppb-l
Standard
toxl20, rb!04199-25ppb-l
Standard
toxl20, rbl04199-50ppb-l
Standard
toxl20, rbl04199-100ppb-l
Standard
toxl20, rbl04199-250ppb-l
Standard
toxl20, rbl04199-500ppb-l
Standard
toxi20, rbl04199-750ppb-l
Standard
tox!20, rb!04199-1000ppb-l
Standard
Rathanoi blank
8 H Analyta
toxl20, rtl, 3M-01-25O ppb-as, 4/19/99 . ^
Analyta
toxi20, rtl, 3M-gl-250 ppb-mad, 4/19/99 toxl20, rtl, 110r-al-2S0 ppb-oajf, 2/22/9
sl*\*
Analyta Analyta
tox!20, rtl, UOr-gl-250 ppb-mad, 2/22/99
- Analyta
texl20, rtl, 10CF-gl-2S0 ppb-na, 2/22/99
Analyta
texl20, rtl, lOCF-gl-250 ppb-ud, 2/22/99
Analyta
toxl20, rb!04199-2S0ppb-ce-4
- QC
aatbanol blank
Analyta
toxl20, rtl, tlM-gC, 4/19/99, dlOOO
Analyta
toxl20, rtl, 92H-g6, 4/19/99, dlOOO
Analyta
toxl20, rtl, 93M-gC, 4/19/99, dlOOO
Analyta
toxl20, rtl, 94M-g6, 4/19/99, dlOOO
Analyta
toxl20, rtl, ISM-gC, 4/19/99, dlOOO
Analyta
toxl20, rbl0419-2S0ppb-ce-l
QC
aathaxwl blank
Analyta
toxl20, rtl, lOf-gC, 4/19/99, dlOOO
Analyta
toxl20, rtl, 7M-gC, 4/19/99, dlOOO
Analyta
* toxl20, rtl, 98H-gC, 4/19/99, dlOOO
Analyta
toxl20, rtl, 99M-g6, 4/19/99, dlOOO
Analyta
toxl20, rtl, W-gC, 4/19/99, dlOOO
Analyta
toxl20, rbl04199-250ppb-cc-6
QC
aatbanol blank
.
Analyta
toxl20, rtl, 91K-g, 4/19/99, dlOOO
Analyta
toxl20, rtl, 3M-g, 4/19/99, dlOOO
Analyta
toxl20, rtl, 94M-g6, 4/19/9, dlOOO
Analyta
toxl20, rtl, SM-gC, 4/19/99, dlOOO
Analyta
toxl20, rbl04199-250ppb-cc-3
QC
Methanol blank
Analyta
toxl20, rtlv 17M-g2, 4/19/99, d2S
Analyta
toxl20, rtl, 18M-g2, 4/19/99, d25
Analyta
toxl20, rtl, 19K-g2, 4/19/99, d25
Analyta
toxl20, rtl, 20M-g2, 4/19/99, d25
Analyta
toxl20, rtl, 21M-g2, 4/19/99, d25
Analyta
toxl20, rtl, 22M-g2, 4/19/99, d25
Analyta
toxl20, rb!04199-250ppb-cc-ll
QC
aatbanol blank
Analyta
toxl20, rtl, 17H-g2, 4/19/99
Analyta
toxl20, rtl, 19K-g2, 4/19/99
Analyta
toxl20, rtl, 19M-g2, 4/19/99
Analyta
toxl20, rtl, 20M-g2, 4/19/99
Analyta
toxl20, rtl, 21M-g2, 4/19/99
Analyta
toxl20, rtl. 22H-g2. 4/19/99
Analyta
toxl20, rbl04199-290ppb-cc-2
QC
aathanol blank
Analyta
t0x120, rtl, 41M-g3, 4/19/99
Analyta
toxl20, rbl04199-wblk-l
Analyta
toxl20, rbl04199-lblk-l
.
Analyta
toxl20, rbl04199-Sppb-l
Standard
toxl20, rbl04199-10ppb-l
Standard
toxl20, rbl04199-2Sppb-l
Standard
toxl20, rbl04199-50ppb-l
Standard
toxl20, rbl04199-100ppb-l
Standard
toxl20, rbl04199-250ppb-l
Standard
t0X120, rbl0419-500ppb-l
Standard
toxl20, rbl04199-750ppb-l
Standard
toxl20, rbl04199-1000ppb-l
'
Standard
Std Cono
5.56 11.10 27. B0 55.60
111.00
278.00 556.00 833.00
1111.00
.
278.00
. 278.00
278.00
278.00
278.00
278.00
5.56 11.10 27.80 55.60
111.00
278.00 556.00 833.00
1111.00
RT
4.950 5.052 5.300 4.985 4.979 4.901 4.924 5.023 5.011 5.028 5.022
4.996 5.033 4.997 5.005 5.000 5.004 5.028
Araa Cono. Flag. Dar
23 121.15 MM 14 70.17 bb
4 8.47 hb
4 6.69 bb 8 30.61 bb 11 48.67 MM 16 82.50 bb
55 314.43 bb 102 599.82 bb 133 786.88 bb 150 889.48 MMX
52 -40
10 -12 -26
13 8
-6 -20
40 228.81 MM
44 252.28 MM
40 223.92 bbx
31 39
173.51 219.73
MM HM
l
7
e>iW<
47 268.17 MM /
45 253.71 MM
-9 ,
5.004
45 256.64 bb
-8
5.236 5.042
4 9.54 bb
47 266.91 bb 's
-4
5.055
5.042 5.060 5.046 5.023 5.024 5.025 5.026
5.026 5.030 5.024 5.028 5.019 S.024 4.994
5.024 5.030
s
5.044 4.786 5.045 5.027 5.020 5.024 4.972
55 314.02 bb
13
36 201.24 bb ^
26 139.32 bb ,
58 336.91 bb i
55 315.14 bb 1
35 196.68 MM )
50 283.47 bb j 9
44 247.94 MM -ll
438 2615.70 bb 570 3410.25 bb 523 3130.06 bb 576 3446.03 bb 539 3224.10 bb 442 2640.17 bb
39 217.34 bb
't
-22
317 1888.62 MM -K)m A
16 79.50 bb
9 36.60 MMX
20 103.61 bbx
19 97.55 bbX
46 264.89 MMX
79 462.30 MMX
85 497.64 bbx
115 676.08 MMX
32 87 -12 -5 -17
-40
-39
IAC
Uc
l -V0
sbd
Analia 062498: quantlatad and prlntad by hoj 5/26/99
BACK TO MAIN
(& J J / S i f o _______________
-- ____________ J ____ :_____:____________________________ ^
Quantify Compound Suanary Kapert toxi20, rtl
- Paga a *
Sample Lit: C:\MABSLYNX\rafcs2.FRO\8asplaDB\OS2599a
Last modified: Wad May 2C 07:17:29 1999
Method:
C:\MA3SL3fNX\rats2.PR0\MethDB\052599
Last Modiflad: Wad May 26 07:18:17 1999
Job Codt
*
..
Printed:
Wad May 26 08:20:32 1999
Compound 2: MS 13
f Wane 1 052S99001 2 052599002 3 052599003 4 052599004 S 052599005 6 052599006 7 052599007
052599008
9 052599009 10 052599010 11 052599011
12 052599012 13 052599013 14 052599014 IS 052599015 16 052599016 17 052599017 IS 052599018 19 052599019 20 052599020 21 052599021 22 052599022 23 052599023
24 052599024 25 052599025 26 052599026 27 052599027
28 052599028 29 052599029 30 052599030 31 052599031 32 052599032 33 0S2599033 34 052599034
35 052599035 36 052599036 37 052599037 38 05259903B 39 052599039
40 052599040 41 052599041 42 052599042 43 052599043 44 052599044 45 052599045 46 05259946 47 052599047
48 052599048 49 052599049 50 052599050 51 052599051 52 052599052 53 052599053
54 052599054 55 052599055 56 0S2599056
57 052599057.
58 052599058 59 052599059 60 052599060 61 052599061 62 052599062 63 052599063 64 052599064 65 052599065 66 052599066 67 052599067
68 052599068 69 0525990(9 70 052599070
Saaplaliat Text
methanol blank
methanol blank
tox!20, rbl04199-wblk-l
tox!20, rbl04199-lblk-l
toxl20, rblO4199-5ppb**1
toxl20, rb!04199-10ppb-l
toxl20, rbl04199-25ppb-l
toxl20, rbl04199-50ppb-l
toxl20, rbl04199-100ppb-l
toxl2Q, rbl04199-250ppb-l
toxl20, rbl04199-500ppb-l
toxl20, rbl04199-750ppb-l
toxl20, rbl04199-1000ppb-l
methanol blank
toxl20, rtl, 3M-gl-250 ppb-ma,r 4/19/99
Vo*
tox!20, rtl, 3H-gl-2S0 ppb-mad, .4/19/99 oil*''4
toxl20, rtl, 110r-gl-250 ppb-as/f, 2/22/99
toxl20, rtl, 110r-gl-250 ppb-ud, 2/22/99
texl20, rtl, lOCF-gl-250 ppb-aa, 2/22/99
toxl20, rtl, lOSF-al-250 ppb-asd, 2/22/99
toxl20, rbl04199-250ppb-cc-4
methanol blank
toxl20, rtl, 81M-g6, 4/19/99, dlOOO
toxl20, rtl, 82M-g6, 4/19/99, dlOOO
toxl20, rtl, 83M-g6, 4/19/99, dlOOO
toxl20, rtl, 84M-g6, 4/19/99, dlOOO
tox!20, rtl, 85M-g6, 4/19/99, dlOOO
toxl20, rb!04199-250ppb-cc-l
methanol blank
toxl20, rtl, 86M-g6, 4/19/99, dlOOO
toxl20, rtl, 87M-g6, 4/19/99, dlOOO
' toxl20, rtl, 88M-g6, 4/19/99, dlOOO
toxl20, rtl, 89M-g6, 4/19/99, dlOOO
toxl20, rtl, 90M-g6, 4/19/99, dlOOO
toxl20, rbl04199-250ppb-cc-6
methanol blank
toxl20, rtl, 91M-g6, 4/19/99, dlOOO
toxl20, rtl, 93M-g6, 4/19/99, dlOOO
toxl20, rtl, 94M-g6, 4/19/99, dlOOO
toxl20, rtl, 95M-g6, 4/19/99, dlOOO
toxl20, rbl04199-250ppb-cc-3
methanol blank
tOX120, rtl, 17M-g2, 4/19/99, d2S
toxl20, rtl, 18M-g2, 4/19/99, d25
tOX120, rtl, 19M-g2, 4/19/99, d25
toxl20, rtl, 20M-g2, 4/19/99, d25
toxl20, rtl, 21M-g2, 4/19/99, d2S
tOX120, rtl, 22H-g2, 4/19/99, d25
toxl20, rbl04199-250ppb-cc-ll
methanol blank
tOX120, rtl, 17M-g2, 4/19/99
toxl20, rtl, 18M-g2, 4/19/99
toxl20, rtl, 19M-g2, 4/19/99
toxl20, rtl, 20M-g2, 4/19/99
toxl20, rtl, 21M-g2, 4/19/99
toxl20 rtl 22M-g2, 4/19/99
tox!20, rbl04199-250ppb-cc-2
methanol blank
toxl20, rtl, 41M-g3, 4/19/99
t0x120, rb!04199-vblk-l
toxl20, rbl04199-lblk-l
toxl20, rbl04199-5ppb-l
toxl20, rbl04199-10ppb-l
toxl20, rbl04199-25ppb-l
toxl20, rbl04199-50ppb-l
toxl20, rbl04199-100ppb-l
toxl20, rbl04l99-250ppb-l
toxl20, rbl04199-500ppb-l
toxl20, rbl04199-750ppb-l
tox!20, rb!04199-1000ppb-l
t Analyte Analyte Analyte Analyte Standard
Standard Standard Standard Standard Standard Standard Standard
Standard Analyte
Analyte Analyta
Analyta Analyta Analyta
Analyta
QC Analyta Analyta Analyta Analyta Analyta Analyta
OC Analyta
Analyta Analyte Analyte
Analyta Analyta
OC Analyta Analyta
Analyta Analyte Analyta
QC Analyta Analyta Analyta Analyta Analyta Analyta Analyta
QC Analyta Analyta Analyta Analyte
Analyte Analyta Analyta QC Analyta Analyte
Analyte Analyta Standard Standard Standard Standard Standard
Standard Standard Standard Standard
8td Cono
5.56 11.10 27.80 55.60 111.00 278.00 556.00 833.00 1111.00
.
278.00
278.00
278.00
278.00
278.00
278.00
5.56 11.10 27.80 55.60 111.00 278.00 556.00 833.00 1111.00
ta
5.471 5.488 5.464 5.488 S.455 5.469 5.465 5.750 5.462 5.475 5.463 5.476 5.468 5.494 5.446
5.470
5.470
'
5.486
5.476
5.460 5.502 5.513 5.489 5.513 5.494 5.515
5.492 5.471 5.490 5.471 S.486
5 .4 8 8
5.485
5.468
5.465 * 5.510 5.501 5.488 5.470 5.464 5.465 5.487
Ara Cone. Flags %Dev
5 12.91 bb
-54
13 72.45 MM
30
21 130.40 bb
17
46 316.68 MM
14
77 540.85 bb
-3
110 788.13 MM
-5
129 922.50 MMX -17
4 0.98 bb
34 226.51 bb*2
39 262.87 bbj
28 180.25 bb"j
26 162.57 bb \
41 277.30 MM .)
42 283.04 bb>
36 240.93 MM -13
-
5 11.89 bb
37 245.31 MM
-12
lAc p s M v
40 267.80 bb
-4
34 228.46 bb
-18
9 40.68 bb \ 6 16.57 MM ) _ I l 13 67.85 bb y K W d 16 88.95 bb
21 14
125.53 80.77
bb bb
///
32 208.72 MM
-25
155 120
138
. 157 161 104 33
1113.48 KM 861.12 bb 987.88 MM
1131.41 bb
1161.29 bb 741.94 bb 216.29 MM
-22
91 643.40 bb
lA C
2 0.00 bbX -100
9 37.86 MMX
36
11 52.34 bbX
-6
22 139.09 bbX
25
34 223.39 bbX -20
61 427.20 bbX -23
71 496.09 bbX -40
74 520.73 bbX -53
Anali 062498: quantitad and printed by hoj 5/26/99
BACK TO MAIN
Quantify Compound t a w y Kapert TQX-120 Kat Liver Dilutions
Sample Lit: S:\IACT-l.PRO\aaapleDB\OSM99a
Last modified: Mon May 17 14:14:20 1999
Method:
8 :\KACT-1.PKO\MethOB\OS1499a
Last modified: Mon Nay 17 14:23:38 1999
Job Code:
Printed:
Tue May It 08:22:40 1999
Compound 2: M4S3
t Ham* 1 051499061 2 051499062
3 051499063
4 051499064
5 051499065 6 051499066 ? 051499067
8 051499068
9 051499069 10 051499070
11 051499071 12 051499072 13 051499073 14 051499074 15 051499075 16 051499076 17 051499077 18 051499078 19 051499079 20 051499080 21 051499081 22 051499082 23 051499083 24 051499084 25 051499085 26 051499086 27 051499087 28 051499088 29 051499089 30 051499090
31 051499091 32 051499092 33 051499093 34 051499094 35 051499095 36 051499096 37 051499097 38 051499098 39 051499099 40 051499100 41 051499101
42 051499102 43 051499103 44 051499104 45 051499105 46 051499106 47 051499107 48 051499108 49 051499109 50 051499110 51 051499111 52 051499112 53 051499113 54 051499114
Saaqjleliat Test
M*OB Blank
MaOH Blank .
Tox-120, RBL04199-H2OBlk-2, 4/19/99
Tox-120, RBL04199-LBlk-2, 4/19/99
Tox-120, RBL04199-5ppb-2, 4/19/99
Tox-120, RBL04199--10ppb-2, 4/19/99
Tox-120, RBL04199-25ppb-2, 4/19/99
Tox-120, RBL04199-50ppb-2, 4/19/99
Tox-120, HBL04199-100ppb-2, 4/19/99
Tox-120, RBL04199-250ppb-2, 4/19/99
Tox-120, RBL04199-500ppb-2, 4/19/99
Tox-120, KBL04199-750ppb-2, 4/19/99
Tox-120, RBL04199-1000ppb-2, 4/19/99
MaOH Blank
Tox-120 23M 62-/ 111:25
.
Tox-120 24M 62 / Dl:25 -
Tox-120 25M 62 / D1:2S
Tox-120 2 G2 / Dl:25
Tox-120, RBL04199-250ppb-CCl, 4/19/99
MaOH Blank
Tox-120 33M 63 / Dl:25
Tox-120 34M 63 / 01:25
Tox-120 35M 63 / 01:25
TOX-120 36M 63 / 01:25
Tox-120 37M 63 / 01:25
Tox-120 3SM 63 / Dl:25
Tox-120 39M 63 / 01:25
Tox-120 40K 63 / 01:25
Tox-120, KBL04199-250ppb-CC3, 4/19/99
MaOH Blank
.
Tox-120 27M 62 / 01:10
Tox-120 29M 62 / Dl:10
Tox-120 3 62 / 01:10
Tox-120 31M 62 / 01:10
Tox-120, XBL04199--250ppb--CC4, 4/19/99
MaOH Blank
Tox-120 42M 63 / 01:10
Tox-120 43M 63 / Dl:10
Tox-120 45M 63 / 01:10
Tox-120 46M 63 / Dl:10
Tox-120 47M 63 / 01:10
Tox-120, RBL04199-250ppb-CC5, 4/19/99
MaOH Blank
Tox-120, RBL04199-H2QBlk-2, 4/19/99
Tox-120, RBL04199-LBlk-2, 4/19/99
Tox-120, RBL04199-Sppb-2, 4/19/99
Tox-120, RBL04199-10ppb-2, 4/19/99
Tox-120, RBL04199-25ppb~2, 4/19/99
Tox-120, RBL04199-50ppb-2, 4/19/99
Tox-120', KBL04199-100ppb-2, 4/19/99
TOX-120, RBL04199-250ppb--2, 4/19/99
Tox-120, KBL04199-S00ppb-2, 4/19/99
Tox-120, HBL04199-750ppb-2, 4/19/99
Tox-120, RBL04139--1000ppb-2, 4/19/99
Type Analyte Analyte
Analyte
Analyte Standard Standard
Standard Standard Standard Standard Standard Standard Standard Analyte Analyte Analyte Analyta Analyte QC
Analyta Analyte Analyte Analyte Analyte
Analyte Analyte Analyte Analyta
QC Analyte Analyta Analyte
Analyte Analyte
QC Analyte Analyte Analyte Analyte Analyte
Analyte QC Analyte Analyta Analyta Standard Standard Standard Standard Standard Standard
Standard Standard Standard
8td Cono
R
5.56 11.10 27.80
55.60 111.00 278.00
556.00 833.00
1111.00
278.00
5.307 5.306 * 5.307 5.307
5.306 5.308 5.308 5.308
5.315 . 5.307
5.306 5.307
278.00
278.00
5.308 5.306 5.307 5.307
278.00
5.306 5.307 5.306 5.307 5.306
5.56 11.10 27.80 S5.60
111.00
278.00 556.00 833.00
1111.00
Arti
613 637 509 473
48 49 47 44 44 35 44 43
1080 1367 1207 1023
66 83 50 63 55
ItaaponM Cone. Flaya
612.731
636.927 509.037 472.806
bbz bbZ bbx bbZ
48.048" '48.561 46.922 43.598 43.676 34.651 44.313
42.794
MMI MHZ MMX
MMX MMX
MMX MMX MMX
1079.684
1367.145
1206.705 1022.573
bbl bbl
bbl bbl
66.125 83.086 SO.209 63.197
55.155
MMX bbl MMX MMX MMX
*
Page 2
Soup 020199, Reviewed and quantitated by drb 5/17/99
BACK TO MAIN
f J s i \ S l ~ i c C ^ _____________________________________________ '
____________________________________--
Quantify Conpound Sunury taper ter120, rtl
_ ,, Page a
tanple Urti C:\MMaunK\rata2.PR0\taapleDB\05259ta
Lait modified: ed May 2 07:17:29 1999
Method:
C:\MMatXMX\rata2.PRO\MathDB\OS2599a
last modified: Wad May 29 07:11:17 1999
Job Coda:
'
. .
Printed:
ad May 2C 0t:20:32 1999
Ceapound 3: MC13
Hama
Samplaliat Text
Type
8td Cone
R
Area Cono. nega %Dv
1 0S2599001 methanol blank
Analyte
2 052599002 methanol blank
Analyte
3 052599003 toxl20, rbl04199~wblk-l
Analyte
4 052599004 toxl20, rb!04199-lblk-l
Analyte
5 052599005 toxl20, rbl04199-5ppb-l
Standard
5.56 5.795
3 1.54 MM -72
6 052599006 toxl20, rbl04199-10ppb-l
Standard
11.10 5.797
5 8.66 MM -22
7 052599007 toxl20, rbl04199-25ppb-l
Standard
27.00 5.765
14 32.23 bb
16
8 052599008 toxl20, rbl04199-50ppb-l
Standard
55.60 5.808
33 82.54 bb
48
9 052599009 toxl20, rbl04199-100ppb-l
Standard 111.00 5.784
S3 137.72 bb
24
10 052599010 toxl20, rbl04199-250ppb-l
Standard 278.00 5.782
116 304.34 bb
9
11 052599011 toxl20, rbl04199-S00ppb-l
Standard 556.00 5.776
221 587.57 bb
6
12 052599012 toxl20, rbl04199-750ppb-l
Standard 833.00 5.767
318 848.84 bb
2
13 052599013 toxl20, rbl04199-1000ppb-l
Standard 1111.00 5.788
369 985.61 bb -11
14 052599014 methanol blank
X J Analyte
3 IS 052599015 16 052599016 17 052599017
toxl20g rtl, 3M-gl-250 ppb-ma/, 4/19/99 ,vt. toxl20, rtl, 3M-gl-2S0 ppb-asd, 4/19/99
toxl20, rtl, llOP-gl-250 ppb-W, 2/22/99
Analyte Analyte Analyte
5.782 5.778 \ - 5.784
105 274.93 bb
118 310.32 MM 104 273.67 MM\
18 052599018 19 052599019
toxl20, rtl, 110P-gl-2S0 ppb-med, 2/22/99 texl20, rtl, 106C-gl-2SO ppb-ne, 2/22/99
Analyte Analyte
5.772 5.765
92 102
241.88 MM 1 r*.1.__t 268.45 bb l ehtertCft
lAt O J Jf a i n
20 052599020 tox!20, rtl, lOCF-gl-250 ppb-med, 2/22/99
Analyta
5.767
120 315.53 MH J
21 052599021 toxl20, rbl04199-250ppb-cc-4
QC
278.00 5.767
101 266.49 MM
-4
22 052599022 nethanol blank
Analyta
23 052599023 toxl20, rtl, lM-gS, 4/19/99, diooo
Analyte
24 052599024 toxl20, rtl, 82M-J6, 4/19/99, dlOOO
Analyta
25 052599025 taxl20, rtl, 3M-g6, 4/19/99, dl000
Analyte
26 052599026 texl20, rtl, 84M-gt, 4/19/99, dlOOO
Analyte
27 052599027 toxl20, rtl, SK-ge, 4/19/99, dlOOO
Analyta
28 052599028 toxl20, rbl04199-2S0ppb-cc-l
QC
278.00 5.792
112 295.72 MM
6
29 052599029 nethanol blank
Analyta
30 052599030 toxl20, rtl, 86K-gf, 4/19/99, dlOOO
Analyta
31 052599031 toxl20, rtl, 7M-g, 4/19/99, dlOOO
Analyta
32 052599032 * toxl20, rtl, am-gf, 4/19/99, dlOOO
Analyte
33 052599033 toxl20, rtl, 89M-g, 4/19/99, dlOOO
Analyte
34 052599034 toxl20, rtl, 90M-gC, 4/19/99, dlOOO
,
Analyta
35 052599035 tox!20, rbl04i99-250ppb-cc-6
QC
278.00 5.782
112 295.27 Kf
6
36 052599036 nethanol blank
Analyta
37 052599037 toxl20, rtl, 91M-gC, 4/19/99, dlOOO
Analyte
38 052599038 toxl20, rtl, 93M-gt, 4/19/99, dlOOO
Analyta
39 052599039 toxl20, rtl, 94M-g6, 4/19/99, diooo
Analyte
40 052599040 texl20, rtl, 5M-g, 4/19/99, diooo
Analyta
41 052599041 toxl20, rbl04199-250ppb-cc-3
QC
278.00 5.795
100 262.77 MM
-5
42 052599042 nethanol blank
Analyta
43 052599043 . toxl20, rtl, 17K-g2, 4/19/99, d25
Analyta
5.781
s 8.69 bb
44 052599044
toxl20, rtl, 18M-g2, 4/19/99, d25
Analyta
45 052599045 toxl20, rtl, 19M-g2, 4/19/99, d25
Analyta
46 052S99046 toxl20, rtl, 20K-g2, 4/19/99, d25
Analyta
47 052599047 toxl20, rtl, 21M-g2, 4/19/99, d25
Analyta
48 052599048
toxl20, rtl, 22M-g2, 4/19/99, d?5
Analyta
49 052599049 toxl20, rbl04199-2S0ppb-ce-ll
QC
278.00 5.013
76 199.37 MM -28
50 052599050 methanol blank
Analyte
51 052599051
toxl20, rtl, 17M-g2, 4/19/99
J 52 052599052 S3 052599053 54 052599054 5S56 0055225S9999005S56
toxl20, rtl, 16M-g2, 4/19/99
toxl20, rtl, 19M-g2, 4/19/99
toxl20, rtl, 20M-g2, 4/19/99 toxl20, rtl, 21M-g2, 4/19/99 toxl20, rtl, 22M-g2, 4/19/99
Analyta Analyta
Analyta Analyta Analyte analyta
5.812 5.791
5.780 5.8X5 5.783 5.785
f36 89.73 MK
28 69.25 bb a }
33 84.01 bb S1 t h k n
30 73.71 bb (
39 29
98.92 72.06
bb bb
>j1
IAC. 0
57 052599057 toxl20, rbl04199-250ppb-cc-2
QC
271.00 5.782
81 210.54 MM -24
58 052599058 nethanol blank
Analyta
59 052599059 toxl20, rtl, 41M*g3, 4/19/99
Analyta
5.789
27 66.29 bb
60 052599060 toxl20, rbl04199'Wblk-l
Analyta
61 052599061 toxl20, rbl04199-lblk-l
Analyte
62 052599062 toxl20, rbl04199-Sppb-l
Standard
5.56 5.741 v
9 17.56 bbX 216
63 052599063 toxl20, rbl04199-10ppb-l
Standard
11.10 5.807 X
7 11.87 bbX
7
64 052599064 toxl20, rbl04199-2Sppb-l
standard
27.80 5.806
. 13 29.34 bbX
6
65 052599065 toxl20, rbl04199-S0ppb-l
Standard
55.60 5.775
11 23.51 bbX -58
66 052599066 toxl20, rbl04199~100ppb~l
Standard 111.00 5.786
41 103.06 bbX -7
67 052599067
toxl20, rbl04199-2S0ppb-l
Standard 278.00 5.790
87 227.94 bbX -18
68 052599068
toxl20, rbl04199-S00ppb-l
Standard 556.00 5.705
150 398.05 bbX -28
69 052599069 70 052599070
toxl20, rbl04199-7S0ppb-l tox!20, rb!04199-1000ppb-l
Standard Standard
1181313..0000
5.787 5.786
204 542.63 bbX -35 252 669.29 bbX -40
f A M l i a 062498: quentieted and printed by hoj 3/26/99
}
i
i
BACK TO MAIN
(4>SS(^k<j ____________ ;__________________________ :____ :______:________________________________ --
Quantify Compound iuua r y Rapprt OSX20 , rtl
- . Pag* 4
S u p l o List! C:\MMSUniX\roto2.FllO\SuploDB\OS2599B Loot aodiflod: od Kay 2 07:17:29 1999
Method:
C:\MfcSSUmx\rata2.9RO\HathDB\052599a
Loot aodiflod: od May 2 07:10:17 1999
Job Cod:
-
Printed:
Wed Hy 26 08:20:32 1999
Coopound 4: FFOS
052599001 052599002 052599003 052599004 052599005 052599006 052599007 052599008 052599009 10 052599010 11 052599011 12 052599012 13 052599013 14 052599014 15 052599015 16 052599016 17 052599017 18 052599018 19 052599019 20 052599020 21 052599021 22 052599022 23 052599023 24 052599024 25 052599025 26 052599026 27 052599027 28 052S99028 29 052599029 30 052599030 31 052599031 32 052599032 33 052599033 34 052599034 35 052599035 36 052599036 37 052599037 38 052599038 39 052599039 40 052599040 41 052599041 42 052599042
43 052599043
44 052599044
45 052599045
46 052599046
47 052599047
48 052599048
49 052599049
50 052599050
51 052599051
52 052599052
53 052599053
54 052599054
55 052599055 56 052599056
57 052599057.
S8 052599058
59 052599059
60 052599060
61 052599061
62 052599062
63 052599063
64 052599064
65 052599065
66 052599066
67 052599067
68 052599068
69 052599069
70 052599070
Sasplelist Text ethanol blank ethanol blank
toxl20, rbl04199-wblk-l toxl20, rbl04199-lblk-l
tox!20, rbl04199-5ppb-l toxl20, rbl04199-10ppb-l toxl20, rbl04199-25ppb-l toxl20, rbl04199-50ppb-l tox!20, rbl04199-100ppb-l toxl20, rbl04199-250ppb-l toxl20, rb!04199-500ppb-l toxl20, rbl04199-750ppb-l toxl20, rbl04199-1000ppb-l methanol blank toxl20, rtl, 3M-gl-250 ppb-as, 4/19/99 toxl20, rtl, 3M-gl-250 ppb-asd, 4/19/99 toxl20, rtl, 110F-gl-250 ppb-ns^ 2/22/99 toxl20, rtl, 110F-gl-2S0 ppb-mad, 2/22/99 toxl20, rtl, 106F-gl-250 ppb~ns, 2/22/99 toxl20, rtl, 106F-gl-250 ppb-mad, 2/22/99 toxl20, rbl04199-250ppb-cc-4 ethanol blank tOX120, rtl, 81M-g6, 4/19/99, dlOOO toxl20, rtl, 82M-g6, 4/19/99, dlOOO toxl20, rtl, 83M-g6, 4/19/99, dlOOO toxl20, rtl> 84M-g6, 4/19/99, dlOOO toxl20, rtl. 5M-g6, 4/19/99, dlOOO toxl20, rbl04199-250ppb-cc-l ethanol blank tox!20, rtl, 86H-g6( 4/19/99, dlOOO t0x 120, rtl, 87M-g6( 4/19/99, dlOOO toxl20, rtl, 88K-gS, 4/19/99, dlOOO toxl20, rtl, B9M-g6( 4/19/99, dlOOO tox!20, rtl. 90M-g6, 4/19/99, dlOOO toxl20, rbl04199-250ppb-cc-6 ethanol blank toxl20, rtl, 91M-g6, 4/19/99, dlOOO toxl20, rtl, 93H-g6, 4/19/99, dlOOO toxl20, rtl, 94H-g6, 4/19/99, dlOOO toxl20, rtl, 95M-g6, 4/19/99, dlOOO toxl20, rbl04199-250ppb-cc-3 ethanol blank . t0x120, rtl, 17M-g2, 4/19/99, d25 toxl20, rtl, 18M-g2, 4/19/99, d25 t0x120, rtl, 19M-g2, 4/19/99, d25 tOX120, rtl, 2QK-g2, 4/19/99, d25 t0x120, rtl, 21M-g2, 4/19/99, d25 toxl20, rtl, 22M-g2, 4/19/99, d25 toxl20, rbl04199-250ppb-cc-ll ethanol blank
toxl20, rtl, 17H-g2, 4/19/99 toxl20, rtl, 18M-g2, 4/19/99 toxl20, rtl, 19M-g2, 4/19/99 t0x120, rtl, 20M-g2, 4/19/99 toxl20, rtl, 21M-g2, 4/19/99 tOXl20, rtl, 22M-g2, 4/19/99 t0x120, rbl04199-250ppb-cc-2 methanol blank toxl20, rtl, 41M-g3, 4/19/99
toxl20, rbl04199-wblk-l t0X120, rbl04199-lblk-l toxl20, rbl04199-5ppb-l toxl20, rbl04199-1Oppb-l toxl20, rbl04199-2Sppb-l tox!20, rbl04199-50ppb-l toxl20, rbl04199-10Oppb-l
t0X120, rbl04199-250ppb-l toxl20, rb!04199-500ppb-l toxl20, rbl04199-7S0ppb-l toxl20, rb!04199-1000ppb-l
Typ
Std Cono
Analyte
Analyte
Analyte
Analyte
Standard
5.56
Standard
11.10
Standard
27.80
Standard Standard
55.60
111.00
Standard
278.00
Standard
556.00
Standard Standard
833.00
1111.00
Analyte
Analyte
Analyte
Analyte
Analyte
Analyte
Analyte
QC 278.00
Analyte
Analyte
Analyte
Analyte
Analyte
Analyte
QC 278.00
Analyte
Analyte
Analyte
Analyte
Analyte
Analyte
QC . 278.00
Analyte
Analyte
Analyte
Analyte
Analyte
QC 278.00
RT
5.329 5.283 5.264 5.268 5.259 5.278 5.277 5.255 5.269 5.284 5.278
5.275 5.266 5.278 5.266 5.255 5.261 5.262 5.245 5.288 5.280 5.283 5.282 5.285 5.284
5.285 5.260 5.274 5.295 5.278 5.273 5.232 5.284 5.263 5.279 5.294 5.288
Analyte Analyte Analyte Analyte Analyte Analyte QC Analyte Analyte Analyte Analyte Analyte Analyte Analyte
QC Analyte Analyte Analyte Analyte Standard Standard Standard Standard Standard
Standard Standard Standard Standard
278.00
278.00
5.56 11.10 27.80 55.60 111.00 278.00 556.00 833.00 1111.00
5.300 5.294 5.280
5.282
5.282 5.284 5.281 5.287 5.300 5.303 5.275
5.284
5.302 5.283 5.279 5.277 5.262 5.254 5.282 5.279 5.2S6 5.277
'
Cono. Flage %Dv
13 34 42 43 92 129 195 445 861 1119 1327
496 504 627 666 516 506 440
5 361 693 494 600 669 405
679 634 566 466 470 404
9 389 311 370 367 365
6 7 16
0.00 bb 0.00 bb 3.40 MM 4.66 bb 41.91 bb 69.73 bb 120.06 bb 310.85 bb 628.49 bb 825.42 bb 984.54 MM
-39
-58
51
25 8
12 13
-1
-11
350.27 bb
356.00 bb
450.18 bb
480.05 bb
365.37 bb 357.63 bb
gnUruI
307.74 bb
u
UtC M w
0.00 bb
246.90 bb
500.27 bb
348.85 bb
429.18 bb 482.06 bb 280.89 bb
' & \ u e A LAC O f 2 1 * 1 `j j
489.72 bb 455.76 bb 403.35 bb 326.97 bb 330.46 bb 279.77 bb
0.00 MM 268.32 bb 209.11 bb 254.08 bb 251.43 bb 250.18 bb
-10
0.00 bb 0.00 bb 0.00 bb
279
81 64 66 54 59 52 339
61
21 29 40 57 87 165 325 590 800 983
184.35 MM
33.46 bb 20.62 bb 22.28 bb 12.57 bb 16.68 bb 11.05 MM 229.96 bb
-34 ft
-17
18.46 bb
0.00 bb 0.00 MMX 1.77 MMX 14.92 bbX 37.99 bbX 97.67 MMX 219.58 bbX 421.63 bbX 582.34 bbX 721.54 bbX
-100 -84 -46
-32 -12 -21 -24 -30 -35
Amelia 062498: quantiatad and printed by hoj 5/26/99
[d s A J f y f o y ___________________
Quantify Csapound Svaaaary Kaport toxl20 , rtl
Saada U s t : C : \ M M S U m x \ n t a 2 . PRO\Suvlal\0S3S99a
Laat sodifiad: Wad May 2f 07:17:2 199
Mathed:
C:\MM8L7HX\rata2.PI\Matid\0S2Sa
Laat aedlflad: Wad May 2C 07:19:17 1999
Job Coda:
Prlntad:
Wad May 2 09:20:32 199
Ccnyonnd S: 19
# NRM 1 052599001 2 052599002 3 052599003
4 052599004 5 052599005 4 052599006 7 052599007 8 052599008 9 052599009 10 052599010 11 052599011 12 052599012 13 052599013 14 0S2599Q14 15 052599015 16 052599016 17 052599017 18 052599018 19 052599019 20 052599020 21 052599021 22 052599022 23 052599023 24 052599024 25 052599025 26 052599026 27 052599027 28 052599028 29 052599029 30 052599030 31 052599031 32 052599032 33 052599033 34 052599034 35 052599035 36 052599036 37 052599037 38 052599038 39 052599039 40 052599040 41 052599041 42 052599042 43 052599043 44 052599044 45 052599045 46 052599046 47 052599047 48 052599048 49 052599049 50 052599050 51 052599051 52 052599052 53 052599053 54 052599054 55 052599055 56 0S2S990S6 57 052599057
58 052599058 59 052599059 60 052599060 61 052599061 62 052599062 63 052599063 64 052599064 65 052599065 66 052599066 67 052599067 68 052599068 69 052599069 70 052599070
faaplaliet Text ethanol blank
typ Analyte
ethanol blank
Analyte
toxl20# rbl04199-wblk-l
Analyte
taxi20, rbl04199-lblk-l
Analyte
toxl20, rb!04199-5ppb-l
Standard
toxl20, rbl04199-1Oppb-1
Standard
toxl20, rb!04199-25ppb-l
Standard
tox!20, rbl04199-50ppb-l
Standard.
toxl20, rb!04199-100ppb-l
Standard
toxl20, rbl04199-250ppb-l
Standard
toxl20, rbl04199-500ppb-l
Standard
toxl20, rbl04199-750ppb-l
Standard
tox!20, rbl04199-1000ppb-l ethanol blank
(V\SC
) X
Standard Analyte
tox!20, rtl, 3M-gl-250 ppb-u,, 4/19/99 lk,-
Analyte
toxl20, rtl, 3H-gl-250 ppb-aad, 4/19/9
Analyte
toxl20, rtl, 110F-gl-250 ppb-oad, 2/22/99 ilw.lV Analyte
toxl20, rtl, 110r-gl-250 ppb-nad, 2/22/99
Analyte
toxl20, rtl, 106r-gl-250 ppb-u, 2/22/99
Analyte
toxl20, rtl, 106I-gl-250 ppb-ud, 2/22/99
Analyte
toxl20, rbl04199-25Oppb-cc-4
QC
ethanol blank
Analyte
toxl20, rtl, 81M-g6, 4/19/99, dlOOO
Analyte
toxl20, rtl, 82M-g6, 4/19/99, dlOOO
Analyte
toxl20, rtl, 03H-g6, 4/19/99, dlOOO
Analyte
toxl20, rtl, 84H-g6, 4/19/99, dlOOO
Analyte
toxl20, rtl, 85M-g6, 4/19/99, dlOOO
Analyte
toxl20, rbl04199-250ppb-cc-l
QC
methanol blank
Analyte
toxl20, rtl, 86M-g6, 4/19/99, dlOOO
Analyte
toxl20, rtl, 87K-g6, 4/19/99, dlOOO
Analyte
* tox!20, rtl, 88H-g6, 4/19/99, dlOOO
Analyte
toxl20, rtl, 89M-g6, 4/19/99, dlOOO
Analyte
toxl20, rtl, 90H-g6, 4/19/99, dlOOO
Analyte
toxl20, rbl04199-250ppb-cc-6
QC
methanol blank
Analyte
toxl20, rtl, 9lM-g6, 4/19/99, dlOOO
Analyte
toxl20, rtl, 93M-g6, 4/19/9$, dlOOO
Analyte
toxl20, rtl, 94K-g6, 4/19/99, dlOOO
Analyte
toxl20, rtl, 95M-g6, 4/19/99, dlOOO
Analyte
tOX120, rbl04199-250ppb-ce-3
QC
methanol blank
Analyte
toxl20, rtl, 17M-g2, 4/19/99, d25
Analyte
* toxl20, rtl, 18H-g2, 4/19/99, <125
Analyte
tOX120, rtl, 19K-g2, 4/19/99, d25
Analyte
tOX120, rtl, 20M-g2, 4/19/99, d25
Analyte
toxl20, rtl, 21M-g2, 4/19/99, d25
Analyte
toxl20, rtl, 22M-g2, 4/19/99, d?5
Analyte
toxl20, rbl04199-250ppb-cc-ll
QC
methanol blank
Analyte
toxl20, rtl, 17H-g2, 4/19/99
Analyte
toxl20, rtl, 18H-g2, 4/19/99
Analyte
toxl20, rtl, 19X-g2, 4/19/99
Analyte
toxl20, rtl, 20M-g2, 4/19/99
Analyte
toxl20, rtl, 21M-g2, 4/19/99 toxl20, rtl, 22H-g2, 4/19/99
Analyte Analyte
toxl20, rbl04199-250ppb-cc-2
QC
methanol blank
Analyte
toxl20, rtl, 41M-g3, 4/19/99
Analyte
toxl20, rbl04199-wblk-l
Analyte
toxl20, rb!04199-- lblk-1
Analyte
toxl20, rb!04199-5ppb-l
Standard
toxl20, rbl04199-1Oppb-1
Standard
toX120, rbl04199-25ppb-l
Standard
toxl20, rbl04199-50ppb-l
Standard
toxl20, rbl04199-100ppb-l
Standard
toxl20, rbl04199-25Oppb-1
Standard
tox!20, rbl04199-500ppb-l
Standard
toxl20, rbl04199-750ppb-l
Standard
tox!20, rbl04199-1000ppb-l
Standard
8td Cono
1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00
*
1.00
1.00
1.00
1.00
1.00
1.00
1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00
KT
5.042 5.022 4.999 5.004 5.022 4.993 4.992 4.993 4.981 4.999 4.993
4.991 5.003 4.992 4.999 5.018 4.999 4.998
5.022 *
5.012
5.025
5.010 5.029 5.041 5.018 5.019 5.046 5.020
5.020 5.024 5.018 4.998 5.013 4.994 5.012
4.995 5.001 5.017 5.022 5.016 4.989 5.000 4.992 4.997 4.991 4.992 4.991
Area
826 ' 1437
1653 1452 1463 1461 1233 1312 1146 1022 1071
1104 1260
197 . 200
219 235 1566
1182
1232
1129
43 47 44 43 29 52 952
488 529 446 570 446 512 1067
518 800 1085 v 1431 * 1233 .1209 1262 1219 1055 1099 983 1054
Cono. Flage %DV
0.66 bb 1.16 bb 1.33 bb 1.17 bb 1.18 bb 1.18 bb 0.99 bb 1.06 bb 0.92 bb 0.82 bb 0.86 bb
0.89 bb 1.01 bb 0.16 bb 0.16 bb . 0.18 bb 0.19 bb 1.26 bb
-34 16 33 17 18 18 -1 6 -8
-18 -14
-11 1
-04 -84 -82 -81
26
0.95 bb
-5
0.99 bb
-1
0.91 bb '
0.03 bb 0.04 bb 0.04 bb 0.03 bb 0.02 bb 0.04 bb 0.77 bb
0.39 bb 0.43 bb 0.36 bb 0.46 bb 0.36 bb 0.41 bb 0.86 bb
0.42 bb 0.64 bb 0.87 bb 1.15 bb 0.99 bb 0.97 bb 1.02 bb 0.98 bb 0.85 bb 0.88 bb 0.79 bb 0.85 bb
-9
-97 -96 -96 -97 -98 -96 -23
r61 -57 -64 -54 -64 -59 .-14
-58 -36 -13
15 -1 -3
2 -2 -15 -12 -21 -15
9mgm S
Aawlia 0C2499: quantlatad and printad by koj 5/26/99
Quantify Calibratio n Itaport toxl20 , rtl
Calibrati! e : \ M M 8 U K X \ r a t a 2 . m o \ C u mM \ 0 S 2 S 9 9 a
Laat andifiad: ad May 26 07:56:39 1999
Printad:
ad May 26 06:21:03 1999
BACK TO MAIN
*> X
Quanttfy Calibration Kaport taxi20, r U
C*libration i C:\MU9UrMX\rata2.9IIO\CuniaBB\052S99a
Laat aodifiad: Wad May 2 07:36:39 199
Frintad:
ad May 2S 09:21:03 1999
-
-
BACK TO MAIN
. *Ba 2 ' *
Quantify Calibration Xaport toxl20 , rtl
'
Calibration: C:\MM9UnoC\rata2.mo\CurvaOB\OS2599a Laat nodifiad: Wad Kay 2 07:56:39 1999
Printad:
Wad May 26 09:21:05 1999
Compound 3 name: M613
:
^
Coefficient o f Determination: 0.983551
Calibration curve: 0.372617 * x + 2.17690
Response type: External Std, Area
Curve type: Linear, Origin: Exclude, Weighting: 1/x, Axis trans: None
]
J
1 TI
5}i
I
I iI
i 1i A a a U a 0624981 quantlatad and printad b y hoj 5/26/99
BACK TO MAIN
Quantify Calibration pert toa!20 , rtl
.
Calibration! C:\MMlUMX\rata2.PIIO\CurvuD8\032599a
taafc aodifiad: W ad May 2 07:54:39 1999
Prlntad:
Had May 2 09:21:03 1999
BACK TO MAIN
Aaalla 062498: quaatiatad and prlntad b y hoj S/24/99
Quantity Calibration Raport toxl20, xtl
Calibration C:\MAS8UNX\xata2.PII0\ClirvaD5\0S2599a
Laat aodlflad: ad May 28 07:58:39 1999
Printad:
od May 28 08:21:05 1999
'
' -
.
BACK TO MAIN
Paga 8
.
Amalia 082498: q u an tiatad and p rin ta d by be] 5/28/99
Method Report
Method filit Last modified:
Compound:
C:\MASSLnoC\rats2.PR0\MethDB\O52599a.mdb Nad May 2 07:18:17 1999(3
1: p o m
Printed:
Had May 28 08:21:19 1999
Compound Nano: Internal Ref.: Quantify Trace: Cone, of Stendardo:
Veer RF Value: dear Peak Factor: Integrate Window Factor:
Peak location
POM [Mona] TIC Cone. A
1.000000, Dioabled 1.000000 2 .0 0 0
Alhtlia
Retention Time (nina): Relativa Retention T i m : T i m Window (nine) :
Peak Selection:
Response
S.010, Enabled 0.000, Disabled 0.304
Nearest
Response Type: Response:
Calibration Curves
. Polynomial Type: Point of Origin: rit Weighting: Axis Transformation:
Concentration
External Areas
Linear Exclude 1/X None
Units: Noise
Peak-to-peak amplitude: Smooth
so
Smoothing: Window size (scans): Number of smooths: Smoothing method:
Baselines
4 Disabled
3
2
Join valley threshold: Reduce peak tailing: ' Raise baseline maximum:
Peak Separation
30.00 70.00 4.00
Draw vertical threshold:
Detect shoulders:
Shoulder threshold:
1
Response Threshold
'
Relative height:
Absolute height: Relative area: Absolute area;
1 0 .0 0 Disabled 10.00
50.00, Enabled 0.00, Disabled 3.00, Enabled 0.00, Disabled
A.c o s / jc./ m
BACK TO MAIN
Faga 1
Method Report
Hathod flit! Last modiiied:
C:\KftSSLyNX\rats2.PR0\HathDB\O52599a.mdb Had Hay 26 07:19:1? 19990
'
-
Compound: Printed:
2: M513
Had Hay 26 09:21:19 1999
/) /
__________________________________________ A l t o t M / i
DLUHWni'iilOV____ Ut or/adu
Compound Hama: Internal Ref.:
Quantity Trace: Cone, of Standard:
MS 13
[None] TIC Cone. A
Osar RF Value: Osar Peak Factor: Integrate Hindov Factor:
1.000000, Disabled 1.000000
2.000
Peak Location
Retention Time (mins): Relativa Retention Time: Time Hindow (mins):
5.551, Enabled 0 .000, Disabled
0.233
Peak Selection:
Hearest
Response
*
Response Type: Response:
External Areas
Calibration Curves
Polynomial Type: Point of Origin: rit Weighting: Axis Transformation:
Linear Exclude l/X
Hone
Concentration
Units:
Noise
Paak-to-peak amplitude:
64
Smooth
Smoothing: Window size (scans): Number of smooths: Smoothing method:
* Disabled 3 2
Baselines
Join valley threshold: Reduce peak tailing: *
Raisa baseline maximum:
30.00 70.00 4.00
Peak Separation
Draw vertical threshold: Detect shoulders: Shoulder threshold!
10.00 Disabled 10.00
Response Threshold
*
Relative height:
absolute height: Relative area: Absolute area;
50.00, Enabled
0.00, Disabled 3.00, Enabled ' 0.00, Disabled
*
BACK TO MAIN
BACK TO MAIN
Method Report
Method file: teat aodlfled:
C:\KA3SLyNX\rets2.PRO\MethDB\052599e.adb Ned May 2 07:18:17 10990
Compound:
3: Mil3
Printed:
Med May 28 08:21:19 1999
Compound Nanai Internal Ref.i Quantify Trace: Cone, of Standardl
M613 (Nona) TIC Cone. A
Ar*ie^.QL2*-
User RF Value: User Peak Factor: Integrate Window factor:
1.000000, Diaabled
1.000000
2.000
Peak Location
Retention Tine (mins): Relative Retention Time: Time Window- (mins) :
S.861, Enabled 0.000, Disabled 0.219
Peak Selection:
Nearest
Response
Response Type: Response:
External Areas
Calibration Curves
Polynonial Type: Point of Origin: Fit Weighting: Axis Transformation:
Concentration
linear Exclude
1/X
None
Units:
Noise
.
Peak-to-peak amplitude:
79
Smooth
Smoothing: Window size (scans): Number of smooths: Smoothing method:
Disabled
3 2
Baselines
Join velley threshold: Reduce peek telling: Relee beseline nexlnun:
30.00 70.00 4.00
Peek Seperetlon
Drew verticsl threshold:
Detect shoulders: Shoulder threshold:
,
Response Threshold
*
10.00 Dlssbled 10.00
'
Relstive height:
Absolute height: Relstive eree: Absolute area:
50.00, Enabled
0.00, Disabled 3.00, Enabled 0.00, Disabled
LAC o s h u k `
. '
'
Peg. 3
Chad Report
Method file: Last modified:
Compound:
C 1\MASSX,TMx\rt*2.PRO\MathDB\OS2599a.db Rad May 26 07:18:17 19990
6: pros
Printed:
Rod May 26 08:21:19 1999
Compound Mama: Internal Ref.: Ouantify Trace: Cone, of Standards:
pros (None] TIC
Cone. A
/t/Jte t e w
dear RF Value: User Peak Factor:
Integrate Mindow rector:
1.000000, Disabled 1.000000 2.000
Peak Location
Retention Tina (alna): Relative Retention Tina: Time Ifindo# (mins):
5.281, Enabled 0.000, Disabled 0.293
Peak Selection:
Nearest
Response
-
Reeponae Type: Response:
External Areas
Calibration Curves
Polynomial Type: Point of Origin: [ Fit Melghting: Axis Transformation:
Linear Exclude l/X None
1 Concentration
units:
-
[ Noise
| Paak-to-paak amplitude:
72
Smooth
! Smoothing: ! Window size (scans): i Number of smooths: 1 Smoothing method:
. Disabled 3 2
.
Baselines
j Join valley threshold: , Reduce peak tailing: Raise baseline maximum:
30.00 70.00 4.00
Peek Separation
] Drew vertical threshold: | Detect shoulders: j Shoulder threshold:
10.00
Disabled 10.00
Response Threshold
%'
| Relative height:
1 Absolute height: 1 Relative area: " Absolute area 2
50.00, Enabled
0.00, Disabled 3.00, Enabled a 0.00, Disabled
i
V
1
BACK TO MAIN
(Jtt osJa l h i_______
Paga A '
Method Report
Method file: Last Modified:
Compound:
Printed:
'
C:\MASSLm\rat2.PRO\M*thDB\052S9..mdb Wed Hey 26 07:11:17 19990
S: IS
Wed May 26 08:21:19 1999
Compound Name: Internal Ref.: Quantify Trace: Cone, of Standards:
User RT Value: User Peak Factor: Integrate Window Factor:
Peak Location
'
Attention Time (mins) : Relative Retention Tins: Tin* Window (mins) :*
Peak Selection:
Response
-
Response Type: Response:
Cslibrstion Curves
Polynomial Type: Point of Origin: Fit Weighting: Axis Transformation:
Concentration
units:
Noise
Peak-to-peak amplitude:
Smooth
*
Smoothing: Window size (scans): Number of smooths: Smoothing method:
Baselines
loin valley threshold: Reduce peak tailing: Raise baseline maximum:
Peak Separation
Draw vertical threshold: Detect shoulders: Shoulder threshold: -
Response Threshold
Relative height: Absolute height:
Relative area: Absolute are*
ZS (None) TIC Fixed
l.OOOOOOp Disabled 1.000000 2.000
5.029, Enabled 0.000, Disabled 0.304
Nearest
External Areas
`'
Linear Exclude 1/X None
83
.Disabled 3 2
30.00 70.00 4.00
10.00 Disabled 10.00
50.00, Enabled
0.00, Disabled 3.00, Enabled 0.00, Disabled
.
A iJ z l . o u m i
BACK TO MAIN
Page 5
r
k L te i_____________________ ,
M assly n x- Sam ple List
Sample List C:\MASSLYNX\rats2.PRO\SampleDB\060199c.SPL Printed: Tue Jun 0116:19:18 1999
'
_____
'
BACK TO MAIN
-
* Pagel
F ile Name
1 060199020
2 060199021
3 060199022 4 060199023
5 060199024 6 060199025
7 060199026 8 060199027 9 060199028 10 060199029 11 060199030 12 060199031 13 060199032 14 060199033 15 060199034 16 060199035 17 060199036 18 060199037 19 060199038 20 060199039 21 060199040 22 060199041
23 060199042 24 060199043 25 060199044 26 060199045 27 060199046 28 060199047 29 060199048 30 060199049 31 060199050 32 060199051 33 060199052 34 060199053 35 . 060199054 36 060199055 37 060199056
3389 006600119999005578
40 060199059 41 060199060 42 060199061 43 060199062 44 060199063 45 060199064 46 060199065 47 060199066 48 060199067 49 060199068 50 060199066 51 060199070 52 060199071 53 060199072 54 060199073 55 060199074 56 060199075 57 060199076
58 060199077 59 060199078 60 060199079
'
File Text
methanol blank
methanol blank
tox120, rbl04199-wblk-2
tox120, rbl04199-lblk-2
tox120, rbl04199-5 ppb-2
tox120, rbl04199-10 ppb-2
tox120, rbl04199-25 ppb-2
tox120, rbl04199-50 ppb-2
tox120, rbl041.99-100 ppb-2
tox120, rbl04199-250 ppb-2
tox120, rt>l04199-500 ppb-2
tox120, rbl04199-750 ppb-2
tox120, rbl04199-1000 ppb-2
methanol blank
tox120,1M -g1,4/19/99
tox120,2M -g1,4/19/99
tox120,3M -g1,4/19/99
tox120,4M -g1,4/19/99
tox120,5M -g1,4/19/99
tox120, rbl04199-250 ppb-co-1
methanol blank
tox120,6M -g1,4/19/99
tox120,7M -g1,4/19/99
tox120,8M -g1,4/19/99
tox120,9M -g1,4/19/99
tox120,10M -g1,4/19/99
tox120, rbl04199-250 ppb-cc-2,5/28/99
methanol blank
'
tox120,11M -g1,4/19/99
tox120,13M -g1,4/19/99
tox120,14M -g1,4/19/99
tox120,15M -g1,4/19/99
tox120, rbl04199-250 ppb-cc-3
methanol blank
tox120,6M -g1, ms, 4/19/99
tox120,6M -g1, msd, 4/19/99
tox120,13M -g1, ms, 4/19/99
tox120,13M -g1, msd, 4/19/99
tox120, rbl04199-250 ppb-CC-4
methanol blank
tox120,3M -g1, ms, 4/19/99
tox120,3M -g1, msd, 4/19/99
tox120,41M -g3,4/19/99, d10
tox120,23M -g2,4/19/99
tox120,24M -g2,4/19/99
tox120, rbl04199-250 ppb-cc-5
methanol blank
tox120,25M -g2,4/19/99
tox120,26M -g2,4/19/99
tox120,27M -g2,4/19/99
tox120,29M -g2,4/19/99
tox120,30M -g2,4/19/99
tox120, rbl04199-250 ppb-co-6
methanol blank
tox120,31M -g2,4/19/99
tox120,33M -fl3,4/19/99
tox120,34M -g3,4/19/99
tox120,35M -g3,4/19/99
tox120,36M -g3,4/19/99
tox120, rb!04199-250 ppb-cc-1
.
MS File
9M IN-POAA-513-613 9M IN -P O A A -51 3 -6 1 3 9M IN -P O A A -51 3 -6 1 3 9M IN-POAA-513-613 9M IN -P O A A -51 3 -6 1 3 9M IN -P O A A -51 3 -6 1 3 9M IN -P O A A -51 3 -6 1 3 9M IN -P O A A -51 3 -6 1 3 9M IN -P O A A -51 3 -6 1 3 9M IN -P O A A -51 3 -6 1 3 9 M IN -P O A A -513 -6 1 3 9M IN-POAA-513-613 9M IN -P O A A -51 3 -6 1 3 9M IN -P O A A -51 3 -6 1 3 9M IN-POAA-513-613 9M IN-POAA-513-613 9M IN-POAA-513-613 9M IN -P O A A -51 3 -6 1 3 9M IN -P O A A -51 3 -6 1 3 9M IN-POAA-513-613 9M IN-POAA-513-613 9M IN-POAA-513-613 9M IN -P O A A -51 3 -6 1 3 9M IN-POAA-513-613 9M IN-POAA-513-613 9M IN-POAA-513-613 9M IN-POAA-513-613 9M IN-POAA-513-613 9M IN-POAA-513-613 9M IN-POAA-513-613 9M IN-POAA-513-613 9 M IN -P O A A -513 -6 1 3 9M IN -P O A A -51 3-61 3 9M IN -P O A A -51 3-61 3 9M IN-POAA-513-613 9M IN-POAA-513-613
99MMIINN--PPOOAAAA--551133--661133
9M IN -P O A A -51 3-61 3 9M IN -P O A A -51 3-61 3 9M IN -P O A A -51 3-61 3 . . 9M IN-POAA-513-613 9M IN-POAA-513-613 9M IN -P O A A -51 3-61 3 9 M IN -P O A A -513-613 9M IN-POAA-513-613 9M IN-POAA-513-613 9M 1N -P O A A -513-613 9M IN-POAA-513-613 9M IN-POAA-513-613 9M IN -P O A A -51 3 -6 1 3 9M IN -P O A A -51 3 -6 1 3 9M IN -P O A A -51 3-61 3 9M IN-POAA-513-613 9M IN -P O A A -51 3 -6 1 3 9M IN-POAA-513-613
9M IN -P O A A -51 3 -6 1 3 9M IN-POAA-513-613 9 M IN -P O A A -513 -6 1 3 9M IN -P O A A -51 3 -6 1 3
In let File
9m in-2 9m ln-2 9m in-2 9m in-2 9m in-2 9m in-2 9m ln-2 9m in-2 9m in-2 9m ln-2 9m ln-2 9m ln-2 9m in-2 , 9min-2 9m in-2 9m in-2 9m in-2 9m in-2 9m in-2 9m ln-2 9m in-2 9m in-2 9m in-2 9m ln-2 9m in-2 9m in-2 9m in-2 9m in-2 9m in-2 9m in-2 9m in-2 9m ln-2 9m ln-2 9m in-2 9m in-2 9m in-2 9m ln-2 9m in-2 . 9min-2 9m ln-2 9m in-2 9m in-2 9m in-2 9m ln-2 9m in-2 9m in-2 9m in-2 9m ln-2 9min-2 9m in-2 9m in-2 9min-2 9m in-2 9m in-2 9m in-2 9m in-2 9m in-2 9m in-2 9m in-2 9m in-2
BACK TO MAIN
f U J U s a _______________ Masslynx-Sample List
____________________ _____ :______ :_______________ :____________________ ' ` Page 2
Sam ple L is t C:\M ASSLYNX\rats2PRO\Sam pleDB\060199C.SPL Printed: Tue Jun 01 16:19:181999
'
F ile Nam e
61 060199080 62 060199081 63 060199082 64 060199083 65 060199084 66 060199085 67 060199086 68 060199087 69 060199088 70 060199089 71 060199090 72 060199091 73 060199092 74 060199093 75 060199094 76 060199095 77 060199096 78 060199097 79 060199098 80 060199099 81 060199100 82 060199101 83 060199102 84 060199103 85 060199104 86 060199105
File Text
methanol blank
10x120,3 7M -g 3 ,4/19/99
10X120,3 8M -g 3 ,4/19/99
to x 1 20,39M -g 3,4/19/99
1ox120,40M -g3,4/19/99
to x 1 20,41M -g 3,4/19/99
tox120, *104199-250 ppb-cc-2
methanol blank
to x 1 20,42M -g 3,4/19/99
to x120,43M -g 3,4/19/99
to x120,45M -g 3,4/19/99
to x120,46M -g 3,4/19/99
to x 1 20,47M -g 3,4/19/99
tox120, *104199-250 ppb-cc-3
methanol blank '
tox120, *l04199-w b lk-2 .
.
tox120, *1 04 1 99-lblk-2
tox120, *104199-5 ppb-2
tox120, *104199-10 ppb-2
tox120, *104199-25 ppb-2
tox120, *104199-50 ppb-2
tox120, *104199-100 ppb-2
to x l2 0, *104199-250 ppb-2
. tox120, *104199-500 ppb-2
tox120, *104199-750 ppb-2
tox120, *104199-1000 ppb-2
MS File
9M IN-POAA-513-613 . 9M IN-POAA-513-613 9M IN -P O A A -51 3-61 3 9M IN -P O A A -51 3 -6 13 9 M IN -P O A A -513 -6 1 3 9 M IN -P O A A -513 -6 13 . 9MIN-POAA-513-613 9 M IN -P O A A -51 3 -6 1 3 9M IN-POAA-513-613 9M IN -P O A A -51 3 -6 13 9M IN-POAA-513-613 - 9M IN-POAA-513-613 9 M IN -P O A A -51 3 -6 1 3 9 M IN -P O A A -51 3 -6 1 3 9M IN-POAA-513-613 9M 1N -P O A A -513-613 9M IN -P O A A -51 3 -6 13 9 M IN -P A A -513 -6 1 3 .. 9MIN-POAA-513-613 9MIN-POAA-513-613 9M IN-POAA-513-613 9 M IN -P O A A -513 -6 1 3 9 M IN -P O A A -513 -6 1 3 9 M IN -P O A A -51 3 -6 1 3 9 M IN -P O A A -513 -6 1 3 9M IN -P O A A -51 3-61 3
In let File
9m ln-2 9m in-2 9m ln-2 9m in-2 9m in-2 9m ln-2 9m in-2 9m ln-2 9m in-2 9m in-2 9m ln-2 9m in-2 9m ln-2. 9m ln-2 9m!n-2 9m ln-2 9m ln-2 9m ln-2 9m in-2 9m ln-2 9m in-2 9m in-2 9m in-2 9m ln-2 9m in-2 9m ln-2
BACK TO MAIN
6 I f t f l _________________ _________________________________________________________________________________
Scanning Method Report
Vaga 1
M athodi
C:\MtSSUrNX\RhTS2.PR0VRCgaDB\9MIM-PaMt-513-S13
Laat Modified: To# JUn 01 3.4:34:06 1999
' '
Printed:
Tua JUn 01 16:19:2 19
'
Solvent Daisy ( nine ) >
0.00
function : 1
M M of 1 Kaaa air ( H P - ) .
Inter Channel Delay ( Secs ) : 0.03
Span ( Daltons ) >
0.00
Start Tina { Kins ) i
.0.00
End Tina { Mins ) :
9.00
Repeats :
1
Channel Parent Daughter Dwell (Sees) Coll Energy teV)
Cone ( V )
1 413.00 169.00 0.30
20
15
function : 2
M9M of 1 Masa Pair t ESP- I
Intar Channel Delay ( Sacs ) > Span ( Daltons ) : Start Tlaa ( Mins I : ' End Tlaa ( Mins ) : Repeats I
Channel Parent Daughter
0.03 0.00 0.00 9.00 1 Dwell
(Secs) Coll Energy (aV)
Cone ( V )
1
513.00 169.00 0.30
' 20
15
Function : 3
MOM of 1 Mesa Pair ( ESP- )
.:
Inter Channel Delay ( Secs ) I Span ( Daltons ) : Start Tlaa ( Mins ) t End Tlae ( Mins ) t Repeats :
Channel Parent Daughter
0.03
o.oo
' '
0.00
9.00
1
Dwell (Secs) Coll Energy (eV)
> Cono ( V )
1 613.00 169.00 0.30
20
15
Function : 4
M M of 1 Maas Pair ( ESP- )
Inter Channel Delay ( Secs ) : Span ( Daltons ) : Start Tlaa ( Mins ) : End Tlaa ( Mins ) : Repeats :
Channel Parent Daughter
0.03
0.00 0.00
9.00
1
Dwell (Secs) Coll Energy (eV)
Cone ( V )
BACK TO MAIN
(a ( t f a c t _______________ ;____________________________________ -__________________ ____________________ - -
Tuning Method Report
Method:
C :\MASSLYNX\RATS2. P R O \ A C Q O D B \ P F O S
Page 1
Printed:
Tue Jun 01 16:20:59 1999
MS
SOURCE ( ESP- )
Capillary
Cone
Extractor
,
RF Lens
Source Block Temp.
Desolvation Temp.
Set Rdbk
3.00 15
5 0.2
150 250
2.97 '-14
0
152 253
Pressures
Analyser Vacuum
Gas Cell
'
Rdbk
1.6e-4 5.1e-3
MSI
LM Resolution HM Resolution Ion Energy , Ion Energy Ramp Lens 6 Multiplier
.\
MS2
LM Resolution HM Resolution Collision Ion Energy Ion Energy Ramp Lens 8 Lens 9 Multiplier
Set Rdbk
15.0 15.0^ 2.0 ? . 0.0 56 650
Set Rdbk
15.0 15.0 20 2.0 0.0 40
0 650
21
38 20
-- -- BACKT0 MAIN FACT: DATA REVIEW SUMMARY FORM
Date: f j l h c
BACK TO MAIN
C (~2. l r\ *1
Quantify Conpound S u m u r y Hapert toxlZO, gl-), r U
S a n p U Lilt: C :\MA3gLma\rt2.PKO\JuplDBM!f0199<l
tt odtfind: Tu* Jun 01 1(:1:01 19*
M*thod:
C:\MUSLymC\nts2.m0\M*th0t\0019o
la.t nodlflad: Wad Jun 02 00:49:03 19 Job Coda:
print**:
Had Jun 02 07:20:47 1999
Conpound 1: OUA
Sanploli.t Taxe
l 060199020
athanol blank
2 060199021
ethanol blank
3 060199022
taxl20, rbl019-ublk-2
4 060199023
taxl20, tbl04l-lblk-2
.
S 060199024
toxl20, rbl0419*-S ppb-Z
6 060199025
COX120, rbl0419-10 ppb-2
? 060199026
toxl20, rbl04199-25 ppb-2 .
8 06019902?
toxl20, rbl04199-50 ppb-2
9 060199028
COX120, rbl04199-100 ppb-2
10 060199029
toxl20, rb104199-250 ppb-2
11 060199030
toxlZO, rbl04199-500 ppb-2
12 060199031
toxlZO, rbl04199-750 ppb-2
13 060199032
toxl20. rbl04199-1000 ppb-2
14 060199033
Mthanel blank
15 060199034
toxl20, lK-gl. 4/19/99
16 060199035
toxl20, 2H-*1, 4/19/99
1? 06019903
toxl20, 3M-gl. 4/19/99
.
19 060199037
toxl20, H-gl, 4/19/99
19 060199038
toxl20, SM-gl, 4/19/99
20 060199039
tOX120, rb!04199-250 ppb-ee-1
21 060199040
athanol blank
22 060199041
tox!20, SM-gl. 4/19/99
23 060199042
toxl20, 7M-gl, 4/19/99
24 060199043
toxI20, 9H-gl, 4/19/99
25 060199044
toxl20, 9M-gl, 4/19/99
26 060199Q4S
toxl20, 10H-gl, 4/19/99
27 060199046
toxl20, rbl04199-250 ppb-cc-2, 5/29/99
28 060199047
athanol blank
29 060199049
toxl20, HM-gl, 4/19/99
30 060199049
toxl20, 13M-gl, 4/19/99
31 060199050
toxl20, 14N-gl, 4/19/99
32 060199051 - toxl20, ISM-gl. 4/19/99
33 060199052
toxl20, rbl04199-250 ppb-ec-3
34 0601990S3
ethanol blank
35 060199054
toxl20, SM-gl, ma, 4/19/99
36 060199055
toxl20, (M-gl, mad, 4/19/99
37 060199056
toxl20, 13H-gl, ma, 4/19/99
38 060199057
toxl20, 13M-gl. mad, 4/19/99
39 060199058
toxl20, rbl04199-250 ppb-cc-4
40 060199059 41 060199060
athanol blank toxl20, 3M-gl, ma, 4/19/99
42 060199061
toxl20, 3M-gl, u d , 4/19/99
43 060199062
toxl20, 41N-g), 4/19/99, dlO
44 060199063
toxl20, 23M-g2, 4/19/99
45 060199064
toxlZO, 24M-g2, 4/19/99
46 060199065
toxl20, rbl04199-250 ppb-cc-S
47 060199066
athanol blank
48 060199067
toxl20, 25M-g2. 4/19/99
49 060199068
toxl20, 2<M-gZ. 4/19/99
'
50 060199069
toxl20, 21M-2, 4/19/99
51 060199070
toxl20, 29H-g2, 4/19/99
52 060199071
toxl20; 30M-g2, 4/19/99
53 060199072
toxl20, rbl04199-250 ppb-cc-(
54 060199073
athanol blank
55 060199074
toxl20, 31M-2, 4/19/99
56 060199075
COX120. 33K--g3, 4/39/99
57 060199076
toxl20, 34H-g3, 4/19/99
58 060199077 ' toxl20, 35M-g3, 4/19/99
59 060199078
toxl20, 36M-g3, 4/19/99
60 060199079
toxl20, rbl04199-250 ppb-ee-1
61 060199080
athanol blank
.
62 060199081
toxlZO, 37M-g3, 4/19/99
63 060199082
toxl20, 3H-g3, 4/19/99
64 060199083
tox!20, 39M-g3, 4/19/99
5 060199084
toxl20. 40M-g3, 4/19/99
66 060199085 . toxl20. 41tl-g3, 4/19/99
67 060199086
toxl20, rbl04199-250 ppb-cc-2
68 060199087
athanol blank
<9 060199088
toxl20, 42M-3, 4/19/99
70 060199089
toxl20, 43M-3, 4/19/99
?l 060199090
toxl20, 45M-g3, 4/19/99
72 060199091
toxl20, 4(X-3, 4/19/99
73 060199092
toxl20, 41H-g3, 4/19/99
74 060199093
toxl20, rbl04l99-250 ppb-ec-3
?5 060199094
ethanol blank
76 060199095
toxl20, rb!04199-ubl k-2
77 060199096
toxHO, rbl04199-lblk-2
78 060199097
toxlZO, rbl04199-5 ppb-2
79 060199098
toxlZO, rbl04199-10 ppb-2
80 060199099
t0x120, rbl04l99-2S ppb-2
81 060199100
tox!20, rbl04199-50 ppb-2
2 060199101
0OX120, rbl04199-100 ppb-2
83 060199102
toxl20, rb!04199-2S0 ppb-2
84 060199103
toxl20, rbl04199-500 ppb-2
85 060199104
toxl20, rbl04199-750 ppb-2
86 060199105
toxl20, rb!04199-1000 ppb-2
.
Typo Analyt*
Analyte Analyte Analyte Standard Standard Standard Standard Standard Standard Standard .Standard Standard Analyte Analyte Analyte Analyte Analyte Analyt* QC Analyte Analyte Analyte Analyte Analyte Analyte QC Analyte Analyte Analyte Analyte Analyte QC Analyte Analyte Analyte Analyte Analyte QC Analyte Analyte Analyte Analyte ' Analyte Analyte QC Analyte Analyt* Analyte Analyte Analyt* Analyt* QC
Analyt* Analyt* Analyt* Analyt* Analyt* Analyt* QC Analyt* Analyt* Analyt* Analyt* Analyt* Analyt* QC Analyt* Analyt* Analyt* Analyt* Analyt* Analyt* QC Analyt* Analyt* Analyt* Standard Standard Standard Standard Standard
Standard Standard Standard Standard
ltd Cone
5.56 11.10 27.80 55.60 111.00 278.00 556.00 33.00 1111.00
* 278.00
, 278.00
278*00
278.00
278.00
270.00
278.00
278.00
271.00
5.56 11.10 27.80 55.60 111.00 278.00 556.00 833.00 1111*00
W 5.082
5.445 4.974 5.052 4.925 5.046 5.038 5.027 5.033 5.020 5.033 5.031 4.978 5.051
4.919 5.034
5.030
5.205 4.730 5.008 5.054 4.989 5.026 4.645 5.435 5.288
4.586 5.033
5.048 5.038 5.050 5.036 5.052 5.287 5.044 5.031 5.049 5.029 5.031 5.039
5.044 5.029 5.046 5.001 5.019 5.021 5.040 5.047 5.032 5.046 5.017 5.025 5.046 5.068 S.0S3 5.049 5.047 5.046 5.033 5.042 5.039 5.049 5.033 5.046 5.031 5.040 5.043
5.077 5.040 5.067 5.063 5.042 5.021 5.025 5.045 5.022 5.025 5.027
ATM 4
Cona. riftai 0.00 bb
3 12 19
9 34 27
65 114 203 280 351
3 5
0.00 bb 24.56 bb 43.28 M4X 12.72 MM 98.26 MMX 69.51 bb 193.86 bb 329.66 bb 599.36 bb 831.57 bb 1046.72 bb
0.0O bb 2.11 bb
678 15
217 25 66 19 8 0 -6
3 0.00 bb 9 14.05 MC
105 303.16 bb
9
4 0.00 bb 6 6.49 bb 9 15.27 MM 6 6.15 MM 5 2.55 HM 110 317.79 bb 2 0.00 bb 9 12.89 bb 7 8.74 bb
14
2 0.00 bb 102 294.80 bb
6
84 84 111 103 112
3 98 90 163 1396 1984 103
239.96 bb 240.30 bb 320.54 bb 299.29 KM 324.29 bb
0.00 bb 281.95 MM 281.92 bb 477.77 bb 4201.20 MM 5974.35 bb 298.39 MM
17 7
995 1026 1442
3S8
424 97 5
512 1365 1788 1658
1508 109
*9 v 1827
1927 1514 1952 1018
109 11
395 907
325 259 423 112
2989.84 MM 3084.63 bb 4338.59 MM 1068.17 bb
1266.11 bb 281.06 bb 0.73 bb
1532.20 bb 4105.96 bb 5384.18 bb 4989.13 bb 4537.13 bb
314.53 bb 13.57 bb
5500.87 bb 5802.57 bb
4556.28 to 5879.27 to 3050.38 bb
314.64 bb 18.85 to
1180.46 bb 2723,34 bb
967.37 bb
768.44 bb 1263.23 bb
325.47 bb
'
r* l
13 13 17
10 18.52 bb 15 33.16 bb 20 47.92 bbX
2 0.00 bb 33 8.03 bbX 27 7.89 MM
48 132.00 MM 98 281.90 HM 196 578.50 bb 256 758.81 bb 344 1025,06 MM
762 -100
217 22
19 1 4
-9 -
dalia 0(2490: quantiatad and printad by ko) */02/f*
i sis n s " -
h SS
G 2*nS m ai "SSSSS
22 5 S 5 2 3 *
iiiiii
2SS5 1 5 S * 333 S 22222SR22S5 22SS2S S5PZSK 2828CK SSS?P2
1 iiiii HHii iiiiii iiiiii miii iiiiii
2 2 2 2 2 2 2 2 2 2 2 2 S 2 S 2 2 S USSKSS 888X82 2 2 2 2 2 2
RIl; ? 3IrtS*
3
SS S S gS P
=iUii
- "223SS
S 2522? 5SSSSS 552322 2S2522 585253 2 5 5 5 2 S
w in io *o * m m o m m*m n n i i n *o n m **o *o immninuv
S 355522
sS2
5ii
S s s SSSSSSooo
S 5 5 "=35333
Aneli 062490: quantiiUd end printed by he) C/02/99
ri
ll lll!ll ll!l l!iilll!illl!illllllllillill!llllili !lill!l ill ll illllllli lllll l i ''-'S222SSS2S2aSH2SSS222SSH2S3SKS2S233533255i8KaaSSSSSSSS32S3S25S2PPP?P2P?22S2SSSS
BACK TO MAIN
Quantify Compound Summary lUport toxl20, gl-3, rtl
Sampl U t : C:\MASL7NX\rt*2.W0\S**loD\060199c
U a t nodlflad: Tu Jun 01 U : 18:01 1999
Mathod:
C:\M*SSLTNX\rCj2.WO\MthBB\06019#a
Laat nodifiad: W*d Jun 02 09:48:03 1999
Job Cod:
mnttd:
*d Jun 02 07:20:47 1999
*
Compound 3: M613
f Nam
laaplaUit T u t
1 060199020
Chino1 blank
2 060199021
Mthanol blank
3 060199022
toxl20, rbl04199-wblk>2
4 060199023
Coxl20, rbl0419S-lblk-2
5 060199024
toxl20, rbl04199-5 ppb-2
6 060199025
t0x120 rbl04199-10 ppb-2
7 060199026
COX120, rb!04199-25 ppb-2
8 060199027
COX120, rbl04199-50 ppb-2
9 060199028
COX120, rbl04199-100 ppb-2
10 060199029
oxl20, rbl04199-250 ppb-2
''
U 060199030
toxl20, rbl04199-500 ppb-2
12 060199031
toxl20, rbl04199-750 ppb-2
13 060199032
toxl20, rbl04199-1000 ppb-2 '
14 060199033
Mthanol blank
15 060199034
COX120, IM-gl, 4/19/99
16 060199035
toxl20, 2M-gl, 4/19/99
17 060199036
toxl20. 3H-gl, 4/19/99.
'
18 060199037
0X120* 4H-1, 4/19/99
19 060199038
COX120* SK-gl, 4/19/99
20 060199039
toxl20. rbl04199-250 ppb-cc-1
21 060199040
Mthanol blank
22 060199041
0x120, St-gl, 4/19/99
23 060199042
oxl20, 7M-gl, 4/19/99
24 060199043
toxl20, 6H-gl, 4/19/99
25 060199044
0X120, 9K~gl, 4/19/99
26 060199045
toxl20, 10M-gl, 4/19/99
27 060199046
oxl20, rbl04199-2S0 ppb-ec-2. 5/21/99
28 060199047
aChanol blank
29 060199048
toxl20, llM-gl, 4/19/99
30 060199049
toxl20, 13M-gl, 4/19/99
31 060199050
0X120, 14N-gl, 4/19/99
32 060199051 COX120, ISH-gl, 4/19/99
33 060199052
toxl20, rbl04199-250 ppb-cc-3
34 060199053
Mthanol blank
35 060199054
toxl20, CM-gl, m m . 4/19/99
36 060199055
toxl20, H-gl, u d , 4/19/99
37 060199056
toxl20, 15M-gl, M , 4/19/99
38 060199057
toxl20, 15M-gl, u d , 4/19/99
39 060199058
COX120, rbl04199-250 ppb-cc-4
40 060199059
Mthanol blank
41 060199060
COX120, 3H-gl, M , 4/19/99
42 060199061
toxl20, 3M-gl, u d , 4/19/99
43 060199062
toxl20, 41N-g3, 4/19/99, dlO
44 060199063
toxl20, 23K-g2, 4/19/99
45 060199064
toxl20, 24M-g2, 4/19/99
46 060199065
Coxl20, rbl04199-250 ppb-cc-5 .
47 060199066
ebano1 blank
48 060199067
0X120, 25M-g2, 4/19/99
49 060199068
tox!20, 2H-g2, 4/19/99
50 060199069
toxl20, 27H-g2, 4/19/99
51 060199070 52 060199071
toxl20, 29H-g2, 4/19/99 0X120, 30H-g2, 4/19/99
'
53 060199072 54 060199073
0X120, rbl04199-250 ppb-cc-6 Mthanol blank
55 060199074
toxl20, 31M-g2, 4/19/99
56 060199075
CoxlZO. 33K-g3, 4/19/99
57 060199076 . toxl20, 34M-g3, 4/19/99
58 060199077
toxl20, 3SN-g3, 4/19/99
59 060199078
COX120, 3<M-g3, 4/19/99
60 060199079
COX120, rbl04199-250 ppb-cc-1
61 060199080
Mthanol blank -
62 060199081
toxl20, 37N-03, 4/19/99
63 060199082
0X120, 3IN-g3, 4/19/99
64 060199083
COX120, 39M-g3, 4/19/99
65 060199084
0X120, 40M-g3, 4/19/99
66 060199085
toxl20, 41M-g3, 4/19/99
67 060199086
oxl20, rbl04199-250 ppb-ce-2
68 060199087
Mthanol blank
69 060199088
0X120, 42H-g3, 4/19/99
70 060199089
toxl20, 43M-gl, 4/19/99
71 060199090
toxl20, 45H-g3, 4/19/99
72 060199091
toxl20, 4<H-g3, 4/19/99
73 060199092
COX12, 47M-g3, 4/19/99
74 060199093
toxl20, rbl04199-2S0 ppb-cc-3
75 060199094
Mthanol blank
76 060199095
0x120. rbl04199-wblk-2
77 060199096
0X120, rbl04199-lblk-2
78 060199097 . toxl20. rb!04199-5 ppb-2
79 060199098
toxl20, rbl04199-10 ppb-2
80 060199099
oxl20, rbl04199-25 ppb-2
81 060199100
COX120, rbl04199-50 ppb-2
82 060199101
COX120, rbl04199-100 ppb-2
83 060199102
COX120, rb!04199-250 ppb-2
84 060199103
tox!20, rbl04199-500 ppb-2
.
85 060199104
COX120, rb!04199-750 ppb-2
86 060199105
COX120, rbl04199-1000 ppb-2
'
*yp*
Std Cono
' Analyta
Analyta
Analyta
Analyto
Standard
5.56
Standard
11.10
Standard
27.80
Standard
55.60
Standard
111.00
Standard
278.00
Standard
556.00
' *Standard 33.00
Standard 1111.00
Analyta
Analyta
Analyta
Analyta
Analyta
' Analyta
QC 270.00
Analyta
Analyta
Analyta
Analyta
Analyta
Analyta
QC 270.00
Analyta
Analyta
Analyta
Analyto
Analyta
QC 278.00
Analyto
Analyta
/ Analyta
Analyta
Analyta
QC 278.00
Analyta
Analyta
Analyte .
Analyta
Analyta
Analyta
QC 278.00
Analyta
Analyta
Analyta
Analyta
Analyta
Analyta
QC 278.00
Analyta Analyta
Analyta
Analyta
Analyta
Analyta
QC 278.00
Analyta
Analyta
Analyta
Analyta
Analyto
Analyta
QC 270.00
Analyta
Analyta
Analyta
Analyta
Analyta
Analyta
QC 278.00
Analyta
Analyta
Analyta
Standard
5.56
Standard
11.10
Standard
27.80
Standard Standard
55.60
111.00
Standard
278.00
Standard
556.00
Standard
833.00
Standard 1111.00
*T
5.743 5.791 5.820 5.798 5.769 5.793 5.700 5.771 5.770
5.773
5,793 *
5.779 5.790 5.783 5.791 5.804 5.798 5.790 5.799 5.797 5.777 5.786 5.010 3.797 5.792 5.760 5.784 5.784 5.813
5.785 5.770
5.806
5.790 5.584
3.796 5.812 5.000 5,790 3.790 5.767
Arm
Cono. T l m g m tOv
3 36.00 bb 14 154.82 bbX
9 102.31 bbX
4 40.84 MM 10 112.24 bb 21 238,05 bb 53 588.77 MM 81 889.42 MC 91 1009.33 MCX
2684 822
-27 1
-14 6 7
-9
21 236.61 bb
-15
20 226.25 bb
It
10 204.21 MM
-27
15 163.64 bb 17 185.98 bb 20 223.84 bb 21 231.57 bb 22 238.60 bb
-14
22 240.26 bb 21 228.26 bb
9 96.59 MM
10 112.16 bb .
20 223.25 HK
-20
3 61.02 MM 11 117.93 MM 13 141.67 bb 10 112.36 MM
8 86.01 MM 25 272.03 MM
11 11729 bb ,
-2
5 60.98 bb . 25 277.42 MM
0
23 252.51 MM
-9
20 219.11 bb 9 103.83 bb
-21
5 51.85 MM 13 171.76 MM 22 243.17 MM 48 533.24 MM 72 797.88 MM 01 092.89 MMX
-7 55 -13 -4 -4 -20
Pag 3
A m i l a 082498: quanelataci and printed fay Ko) 4/02/99
BACK TO MAIN
Quantify Confound Summary Raport toxl20, gl-3, rtl
Sampla U t ! C:\MMSLTHX\rata2.PRO\SMgilaOa\OS019flo
Laat modifiad: Tu* Jun 01 16:18:01 1999
Mathod:
C:\HASSLYHX\rata2.PTO\MathOS\0C0199a
Laat modifiad: Nad Jun 02 04:49:03 1999
Job Coda:
Printad:
Nad Jun 02 07:20:4? 1999
_
Coanpound 4: 18
Mama *" 1 060199020
2 060199021 3 060199022 4 060199023 3 060199024 6 060199025 7 060199026 8 060199027 9 060199020 10 060199029 11 060199030 12 060199031 13 060199032 14 060199033 15 060199034 16 060199035 17 060199036 10 060199037 19 060199038 20 060199039 21 060199040 22 060199041 23 060199042 24 060199043 25 060199044 . 26 060199045 2? 060199046 28 060199047 29 060199048 30 060199049 31 060199050 32 060199051 33 060199052 34 060199053 35 060199054 36 060199055 3? 060199056 38 060199057 39 060199058 40 060199059 41 060199060 42 060199061 43 060199062 44 060199063 45 060199064 46 060199065 47 060199066 48 06019906? 49 060199068 50 060199069 51 060199070 52 060199071 53 060199072 54 060199073 55 060199074 56 0 6 0 1 9 9 0 7 5 57 060199076 58 060199077 59 060199078 60 060199079 61 060199080 62 060199081 63 060199082 64 060199083 65 060199084 66 060199085 67 0601990B6 68 060199087 69 060199088 70 060199089 71 060199090 72 060199091 73 060199092 74 060199093 75 060199094 76 060199095 77 060199096 78 060199097 79 060199098 80 060199099 01 060199100 82 060199101 83 060199102
84 060199103 05 060199104 86 060199105
Saaplaliat Taxt
mathanol blank
mat h a n d blank
toxl20, rbl04l99-wblk-2
toxl20, rb!04199-lblk-2
toxl20, rbl04199-5 ppb-2
toxl20f rbi04199-10 ppb-2
toxl20, rbl04199-2S ppb-2
toxl20, rb!04199-50 ppb-2
toxl20, rbl04199-100 ppb-2
toxl20, rbl04199-2S0 ppb-2
*
toxl20, rbl04199-500 ppb-2
'
toxl20, rbl04199-750 ppb-2
toxl20, rbl04199-1000 ppb-2 '
mathanol blank
toxl20, IK-gl, 4/19/99
toxl20, 2M-gl, 4/19/99
toxl20, 3M-gl, 4/19/99 . *
tOX120, 4H-gl, 4/19/99
tOX120, SM-gl, 4/19/99
COX120, rbl04199-250 ppb-ee-1
mathanol blank
tox!20, 6M-gl, 4/19/99
toxl20, 7K-gl, 4/19/99
toxl20, 8M-gl, 4/19/99
tox!20, 9M-gl, 4/19/99
toxl20, 10M-gl, 4/19/99
toxl20, rbl04199-250 ppb-cc-2, 5/28/99
mathanol blank
toxl20, llM-gl, 4/19/99
toxl20, 13H-gl, 4/19/99
tox!20, 14M-gl, 4/19/99
* toxl20, 15M-gl, 4/19/99
tox!20, rbl04199-250 ppb-cc-3
mathanol blank
toxl20, 6M-gl, ma, 4/19/99
toxl20, 6H-gl, mad, 4/19/99
tox!20, 13M-gl, ma, 4/19/99
toxl20, 13H-gl, mad, 4/19/99
toxl20, rbl04199-250 ppb-cc-4
mathanol blank
taxl20, 3M-gl, ms, 4/19/99
toxl20, 3M-gl, mad, 4/19/99
tox!20. 41M-g3, 4/19/99, dlO
toxl20, 23M-g2, 4/19/99
toxl20, 24H-g2, 4/19/99
toxl20, rbl04199-250 ppb-cc-5
mathanol blank
tox!20, 25H-g2, 4/19/99
toxl20, 26M-g2, 4/19/99
toxl20, 27M-g2, 4/19/99
toxl20* 29M-g2, 4/19/99
toxl20, 30K-g2, 4/19/99
toxl20, rbl04199-250 ppb-cc-6
mathanol blank
toxl20, 31H-g2, 4/19/99 COX120, 33H -g3, 4/19/99
toxl20, 34M-g3, 4/19/99
toxl20, 35H-g3# 4/19/99
toxl20, 36M-g3, 4/19/99
toxl20, rbl04199-250 ppb-cc-1
mathanol blank
*
toxl20, 37H-g3, 4/19/99
toxl20, 38M-g3, 4/19/99
toxl20, 39H-g3, 4/19/99
toxl20, 40H-g3, 4/19/99
toxl20, 41M-g3, 4/19/99
toxl20, rbl04199-2S0 ppb-cc-2
mathanol blank
tox!20, 42H-g3, 4/19/99
toxl20, 43M-g3, 4/19/99
tOXl20, 45M-g3, 4/19/99
tOx!20, 46M-g3, 4/19/99
toxl20, 47M-g3, 4/19/99 toxl20f rbl04199-250 ppb-cc-3
mathanol blank
tox!20, rbl04199-wblk-2
toxl20, rbl04199-lblk-2
toxl20, rbl04199-5 ppb-2
toxl20* rbl04199-10 ppb-2
toxl20, rbl04199-25 ppb-2
toxl20, rbl04199-50 ppb-2
Cox120, rbl04199-100 ppb-2
COX120, rbl04199-250 ppb-2
toxl20# rbl04199-500 ppb-2
toxl20, rb!04199-750 ppb-2
toxl20, rb!04199-1000 ppb-2
Saga 4
' *
typ Analyt. Analyta Analyta Analyta Standard Standard Standard Standard Standard Standard Standard `Standard Standard Analyta Analyta Analyta Analyta Analyta Analyta QC
Analyta Analyta Analyta Analyta Analyta Analyta QC
Analyta Analyta Analyta Analyta Analyta QC
Analyta Analyta Analyta Analyta Analyta QC Analyta Analyta Analyta Analyta Analyta Analyta QC Analyta Analyta Analyta Analyta Analyta Analyta QC Analyta Analyta Analyta Analyta Analyta Analyta QC
Analyta Analyta Analyta Analyta Analyta Analyta
ac
Analyta Analyta Analyta Analyta Analyta Analyta QC Analyta Analyta Analyta Standard Standard Standard Standard Standard Standard Standard Standard Standard
Std Cono
1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 .
1.00
' 1.00
1.00
1.00
1.00
1.00
1 a 00
1.00
1.00
1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 l.OO
**
5.061 5.05? 5.024 5.031 5.018 5.032 5.021 5.027 5.014 5.026 5.003
5.024 5.016 5.046 5.028 5.010 5.024
5.023 5.014 5.024 5.004 5.005 5.021
5.028 5.014 5.023 5.023 5.027
$.021 5.033 5.023 5.030 5.024
5.016 5.025 5.022 5.023 5.025 5.033
5.016 5.024 5.018 4.996 5.013 5.015
5.042 5.004 5.019 5.012 5.019 5.018
5.025 5.021 5.042 5.019 5.005 5.014
5.022 5.049 5.018 5.026 5.033 5.015
5.050 5.034 5.039 5.036 5.037 5.023 4.997 5.018 5.016 5.020 4.999
Arm* Cone. rlmg %Dv
862 1055 1051 1057 1119
909 1047
906 907 898 972
779 873 72 909 764 70
691 730 809 700 870 953
702 684 783 667 963
768 763 696 810 948
036 877
93 466 555 886
541 523 1219 779 536 895
591 90S 509 . 457 546
,.Nv ! 517 483 630 466 471 940
659 566 535 615 534 136
913 923 923 974 1061 08 978 843 750 764 050
0.92 bb 1.13 bb 1.12 bb 1.13 bb 1.20 bb 0.97 bb
1.12 bb 0.97 bb 0.97 bb 0.96 bb 1.04 bb
-8 13 12 13 20
-3 12 -3 -3 -4
4
0.83 bb 0.93 bb 0.93 bb 0.97 bb 0.82 bb 0.93 bb
-17 -7 -7 -3
-18 . -7
0.74 bb 0.7* bb 0.87 bb 0.75 bb 0.93 bb 1.02 bb
-26 -22 -13 -25
-7
2
0.75 bb 0.73 bb 0.84 bb 0.71 bb 1.03 bb
-25 -27 -16 -29
3
0.82 bb 0.82 bb 0.74 bb 0.87 bb 1.01 bb
-18 -1B -26 -13
1
0.89 bb 0.94 bb 0.10 bb 0.50 bb 0.59 bb 0.95 bb
-11 -6
-90 -50 * -41
-5
0.58 bb 0.56 bb 1.30 MH 0.83 bb 0.57 bb 0.96 bb
0.63 bb 1.05 bb 0.54 bb 0.49 bb 0.50 bb 0.87 bb
-42 -44
30* -17 -43
-4
-37 5
-46 -51 -42 -13
0.55 bb
0.52 bb 0.67 bb 0.50 bb 0.50 bb 1.01 bb
-45 -48 -33 -50 -50
1
0.71 bb 0.61 bb 0.57 bb 0.66 bb 0.57 bb
0.89 bb
-29 -39. -43 -34 -43 -11
0.90 bb 0.99 bb 0.99 bb 1.04 bb 1.14 bb 0.06 bb 1.05 bb 0.90 bb 0.81 MM 0.82 bb 0.91 MM
-2 -1 -1
4 14 -14
5 -10 -19 -18
-9
Amalia 042498: quantiatad and printad by hoj 4/02/99
BACK TO MAIN
<o f e
A ________________________________________'_____________ _ _ _ _________________________ _
Quantify Calibration Itaport toxl20, 1-3, rtl
Vmq* 1
Calibration: C:\HM2LYNX\rata2.PM\Ciirvo09\090199a Loot nodlflod: Usd JUn 02 07:10:00 1000
Prlntod:
Wod Jun 02 07:20:01 1999
.
*
Inolia 002490: quantlatad and prlntod b y hoJ 0/02/90
Quanti fy Callbration Xaport toxl20, gl-3, ttl
callbration : C :\MM0lYMC\rata2.M0\CurvoDB\0019e
Laat aodiflad: ad Jun 02 07)10:0 loto
rrintad:
ad Jun 02 07:20:51 1900
BACK TO MAIN
:
Quantiiy Calibratlon m o r e toxlJO, gl-2. rtl
Calibratlon: C:\HhoaLTMX\raea2.mo\airvaDa\040199a
taat aodlfiad: ad Jun 02 07:10:04 1290
Prlntad:
Wad Jun 02 07:20:31 1992
'
BACK TO MAIN
. p9 4
* a U a 042490: quantlatad and prlntad by ho) 4/02/99
SOUP 020199: FA CT E quipm ent Log 99014
Q uattro II, #6414E
PAGE
DATE: 06/08/99
ANALYST: drb
STUDY NUMBER:TOX-120 M ATRIXrRat Liver DESCRIPTION: POAA analog check FACT M ethod: ETS-8-5.0 .
Injection volume: 10 uL
Cycle time: 9 min. Electrode: Z-Sprav______
MobUe Phase:
(A)2.0 mM NH4CH3C02
(B) MeOH ___________
T N -A -orstndrd num ber (A) 99012-10-9________
ffiYTN-A-2540_________
Prepared (date/by): E x p ires:
/A) 06/04/99 drb
(A) 10/99______________
ffi) 06/03/99 drb (B) 10/99______________
Store at room tem p? ffj or N
O th er_______ ;__________________
G uard colum n: None__________________
Part number: na_________
Lot num ber na________________________
A nalytical colum n: Keystone B etasil_________________________ '___________
Size: 2 x 50 mm________________ Type: C l 8____________ Sphere size: 5 urn _______
P art number: 055-701-2__________ Lot number P7LQ4
Serial number:0481175Z
A nalytical p aram eters:
Drying gas (L /h r ) :J 5 Q _ ESI nebulizing gas OJbr) 15
LC Flow rate: 300 nTVmin Back pressure: 55 b ar
Sheath gas (L/br)_0____ .
H PLC solvent g rad ien t: 9m in-2
Tim e fmin.1
DJOLQQ__________ 2 ) 1.00 3) 4.50
4) JL5Q______ 517.00________
%B
4 0 .0 4 0 .0 9 5 .0
210. 40.0_
i
M asslynx - Sam ple List
Sample List: C:\MASSLYNX\soup-rats.pro\SampleDB\060899a.SPL Printed: Tue Jun 0815:56:341999
BACK TO MAIN
Page 1
File Name
1 060899001 2 060899002 3 060899003 4 060899004 5 060899005 6 060899006 7 060899007 8 060899008 9 060899009
R ie Text
M S F ile In le t R le B ottle
MeOH Blank
.
Tox-120, RBL04199-UverBlk-1,4 /1 9 /!
Tox-120, RBL04199-10 ppb -1 ,4 /1 9/S
Tox-120, RBL04199-100 ppb -1 ,4 /1 9 ,
Tox-120, RBL04199-1000 ppb -1 ,4 /1 !
MeOH Blank
TO X -120,19M G2
MeOH Blank
T O X -120,35M G 3
POAA-51: POAA-51! POAA-51: POAA-51: POAA-51: POAA-51: POAA-51: POAA-51: POAA-51:
9m in-2 9m in-2 9m in-2 9m in-2 9m In-2 9m ln-2 9m in-2 9m in-2 9m in-2
91 1 2 3 4 91 5 92 6
4 V olum e S a m p le T C one A
10 Analyte 10 Analyte 10 Analyte 10 10 Standard 100 10 Standard 1000 10 Analyte 10 Analyte ' 10 Analyte 10 Analyte
i
Acumina Method Report -
uthedi
ci\KMSLnxNAODr>UTa.movacgin\peAA-5l3-(i3
M a t Modifiedi Tua Jun 0* 13:55>54 U M
Printed!
Sua Jun 0( 15i00>4 1*3
folvant Delay ( mina ) t
0.00
'
Fonction t 1
KAM of 1 Kaaa Fair ( W - )
Inter channel Dalay ( Seca ) i Span ( Daltons ) i Start Tina ( Mins ) Snd Tina ( Mina ) i Repasta
Channel Parent Daughter
0.03
0.00 0.00
9.00
1
Dwell
(Seca) Coll Energy (eV)
Cone ( V )
1 413.00 103.00 '0.25
20
IS
Function i 2
MAM of 1 Maas air ( RAF- )
Inter Channel Delay ( Sec* ) > Span { Dalton* ) t Start Time ( Kins ) < *
End Tima ( Kin* ) i Repeats t
Channel Parent Daughter
0.03
0a00
0.00 9.00
*
1
Dwell (Secs) Coll Energy (eV)
Cono ( V )
1 513.00 169.00 0.25
20 '
Function t 1
MRM of 1 ** Fair { MSP- )
Intar Channel Dalay ( Secs ) > Span ( Daltons ) Start Tine ( Mins ) i Snd Tine ( Mins ) > Repeats >
Channel Parent Daughter
0.03 0.00 0.00 9.00 1 Dwell
(Secs) Coll Energy (eV)
Cone ( V )
1 513.00 159.00 0.25
20
15
Function t 4
M m of 1 M a s Fair ( BSP- )
Inter Channel Delay ( Saca ) i Spaa ( Daltons ) i Start Tine ( Mina ) t Snd Tine ( Mina ) i Repaata t
Channel Parent Daughter
0.03 0.00 0.00 9.00 1 Dwell
(Secs) Coll Energy
(eV)
Cone ( V. )
1 353.00 119.00 0.20
20
15
Function t S
M m of 1 Naan Fair ( BSP- )
Inter Channel Dalay ( Sec* ) i Span ( Daltons ) i Start. Tine ( Mina ) i Bnd Tine ( Mina ) i Repeats t
Channel Parent Daughter
0.03 0.00
0.00 9.00 1 Dwell
(Secs)
1
453.00 169.00
0.20
20
M
(eV) Cone ( V )
IS
Function 1 4
M m if 1 Maas Fair ( BSP- )
Inter channel Delay { Secs ) i span { Daltons ) i Start Tine ( Mins ) > Snd Tine ( Mina ) i Repeats i
Channel parent Daughter
0.03 0.00 0.00 9.00 1
H
2
(Secs) Coll Energy (eV)
Cone ( V )
1 553.00 169.00 . 0.20
20
15
notion 7
MAM et 1 Masa Fair l rat- )
Inter Channel Delay ( Saca ) Span ( Daltons ) i start Tine ( Mina > tnd Tina ( Mins ) > Repeats i
0.03
0.00 o.OO j.OO 1
Channel Parent Daughter Dwell (Seca) Coll energy (eV)
1
553.00 159.00 0.20 '
20
Cone { V ) 15
BACK TO MAIN
.0
1
Tuning Method Report
-
Method:
C :\MASSLYNX\SOUP-RATS. PR O\ACQUDB\PFOS
Printed:
Tue Jun 08 16:43:16 1999
BACK TO MAIN
O b /D & f
Page 1
SOURCE ( ESP- )
1 Capillary j Cone
Extractor . RF Lens
Source Block Temp.
1 Desolvation Temp.
1
1
| Pressures
.
1
Analyser Vacuum
1. ] Gas Cell
Set Rdbk
3.00
60 5 0.2 150 250
3.01 -60 -7
153 252
Rdbk
2.2e-4 4.9e-3
'
MSI
LM Resolution HM Resolution ion Energy Ion Energy Ramp Lens 6
Multiplier
,\
MS2
LM Resolution HM Resolution Collision Ion Energy Ion Energy Ran Lens 8 Lens 9 Multiplier
Set Rdbk
14.0 14.0 2.0 0.0
52
650
Set Rdbk
15.0 15.0 45 2.0 0.0 40 0 650
43
38 43
AMELIA062498: FACT Equipment Log 99016
Q uattro H , #6448E
PAGE
DATE: 06/09/99
ANALYST: sah/mee
STUDY NUMBER Tox-120, MATRIX: rat liver DESCRIPTION: IP
FACT Method: cts-M -5.0________
Injection volume. lOuL
Cycle tim e: 13.5 min.
Electrode: Z-Spray
M obile Phase:
(A)2.0 mM NH4CH3CQ2
(B) MeOH
TN-A- or stndid num ber (A ) 99012-10-6________
(B) TN-A-2647
Prepared (date/by):
(A) 5/21/99 ias__________
(B) 6/7/99 sah
Expires:
(A) 11/99_______________
(B) 12/99________
Store at room temp? [7] or N
O ther:
G uard colum n: None___________________
Part num ber n a ________
Lot number na
A nalytical colum n: keystone betasil____________ _
Size: 2 .
x
100mm>'
"
Type: cl8
Sphere size:5 um .
Part num ber 105-705-2______ Lot num ber p7h!2 Serial num ber 068640b
A nalytical param eters: Drying gas (L/hr): 300
ESI nebulizing gas (L/hr) 15
LC Flow rate: 300 uL/min. Back pressure: 80 bar
Sheath gas (L/hr)J)
H FLC solvent g radient: p fb sl
Tim e (m in.) )1 0 . 0 0 )2 1 . 0 3 8.5
5) 1 1 . 0 )6 1 2 . 0
.
%B 5 5 90 90 5
BACK TO MAIN
/ngg
--
A ffo Hfum JM IMJs i
M asslynx - Sam ple U s t
-
Sample Ust: C:\MASSLYNX\ameOa'rats.PRO\SampleOB\06999a.SPL Printed: W ed Jun 0 915:01:191999
File Nam e
File Text
1 060999001
methanol blank
'
2 060999002
methanol blank
3 060999003
tox120, rt02229-H2Oblk-1
4 060999004
tox120, rbl02229-Livertolk-1
5 060999005
tox120, rbi02229-5ppb-1
6 060999006
tox120, rbl02229-10ppb-1
7 060999007
tox120, rbl02229-25ppb-1
8 060999008
tox120, rbl02229-50ppb-1
9 060999009
tox120, rbl02229-100ppb-1
10 060999010
tox120, rt>l02229-250ppb-1
11 060999011
tox120. rbl02229-500ppb-1
12 060999012
tox120, rbl02229-750ppb-1
13 060999013
tox120, rbl02229-1000ppb-1
14 060999014
methanol blank
15 060999015
to x 1 20 ,106F-G 1-250ppb-ms, 2/22/99
16 060999016
to x 1 20 ,106F-G1-250ppb-msd, 2/22/99
17 060999017
tox120,3M -G1-250ppb-m s, 4/19/99
18 060999018
tox120,3M -G1'250ppb-m sd, 4/19/99
19 060999019
tox120, rbl02229-250ppb-cc1
20 060999020
methanol blank
21 060999021
tox120,153F-G 4,2/22/99
22 060999022
tox120,154F-G 4,2/22/99
23 060999023
tox120,155F-G 4,2/22/99
24 060999024
tox120, rbl02229-250ppb-cc2
25 060999025
methanol blank
-
26 060999026
to x120,157F-G 4,2/22/99
27 060999027
tox120,158F-G 4,2/22/99
28 060999028
tox120,159F-G 4,2/22/99
-
29 060999029
tox120, rbl02229-250ppb-cc3
30 060999030
methanol blank
31 060999031
tox120,57M -G 4,4/19/99
32 060999032
to x 1 2 0 ,5 8 M -6 4 ,4/19/99
33 060999033
tox120,59M -G 4,4/19/99
34 060999034
tox120, rbl02229-250ppb-cc4
35 060999035
methanol blank
36 060999036
tox120,61M -G 4,4/19/99
37 060999037
tox120,62M -G 4,4/19/99
38 060999038
tox120,63M -G 4,4/19/99
39 060999039
tox120, rbl02229-250ppb-cc5
40 060999040
methanol blank
41 060999041
tox120,169F-G 5,2/22/99
42 060999042
to x120.170F-G 5,2/22/99
43 060999043
tox120,171F-G 5,2/22/99
44 060999044
tox120, rbl02229-250ppb-cc6
45 060999045
methanol blank
46 060999046 . tox120,173F-G 5,2/22/99
47 060999047
to x120.174F-G 5,2/22/99
48 060999048
to x120,175F-G 5,2/22/99
49 060999049
tox120, rbl02229-250ppb-cc7
50 060999050
methanol blank
51 060999051
tox120,73M -G 5, d 25 0,4/19/99
52 060999052
tox120,74M -G 5, d 25 0,4/19/99
53 060999053
tox120,75M -G 5, d 25 0,4/19/99
54 060999054
tox120, rbl02229-250ppb-cc8
55 060999055
methanol blank
56 060999056
tox120, rbl02229-H2Oblk-2
57 060999057
tox120, rbl02229-Uverblk-2
58 060999058
tox120, rbl02229-5ppb-2
59 060999059
tox120, rbl02229-10ppb-2
60 060999060
tox120, rbl02229-25ppb-2
BACK TO MAIN
<7
Page 1
MS File
PFHS PFHS PFHS PFHS PFHS PFHS PFHS PFHS PFHS PFHS PFHS PFHS PFHS PFHS PFHS PFHS PFHS PFHS PFHS PFHS PFHS PFHS PFHS PFHS PFHS PFHS PFHS PFHS PFHS PFHS -PFHS PFHS -PFHS -PFHS PFHS -PFHS -PFHS -PFHS -PFHS -PFHS ` -PFHS -P F H S -PFHS -PFHS -PFHS -PFHS -P F H S -P F H S -P F H S -P F H S -P F H S -P F H S -P F H S -P F H S -P F H S -P F H S -P F H S -P F H S -P F H S -P F H S
In le t File
p fb s l p fb s l p fb s l p fb s l p fb s l p fb s l pfbsl p fb s l p fb s l p fb s l p fb s l p fb s l p fb s l pfbsl p fb s l p fb s l pfbsl p fb s l p fb s l p fb s l p fb s l p fb s l pfbsl pfbsl pfbsl p fb s l p fb s l pfbsl pfbsl p fb s l p fb s l pfbsl pfbsl pfbsl pfbsl r pfbsl p fb s l p fb s l p fb s l p fb s l p fb s l p fb s l pfbsl p fb s l pfbsl pfbsl pfbsl pfbsl pfbsl pfbsl p fb s l pfbsl pfbsl pfbsl pfbsl p fb s l pfbsl p fb s l pfbsl pfbsl
B ottle
91 91 1 2 3 4 5 6 7 8 9 10 11 92 12 13 14 15 81 92 16 17 18 82 93 19 20 21 83 93 22
23 24 84 94 25 26 27 85 94 28 29 30 86 95 31 32 33 87 95 34 35 36 88 96 37 38 39 40 41
> W ml IcM Z fM - u ( / M
Masslynx - Sampie U st
'
Sample Ust: C:\MASSLYNX\am elia-rat3.PRO\Sam pleDB\060999a.SPL Printed:W edJun 0 9 1 5 :0 1 :1 9 1 9 9 9
File Nam e
61 060999061 62 060999062 63 060999063 64 060999064 65 060999065 6 6 060999066
F lIe T ex t
tox120, rb!02229-50ppb-2 tox120, rbt02229-100ppb-2 ta x i20, rbl02229-250ppb-2 tox120, rb!02229-500ppb-2 1 0 x120, rbl02229*750ppb-2 tox120, rb!02229-1000ppb-2
v / y BACK TO MAIN
/n&z _ .
Page 2
MS File
PFHS PFHS PFHS PFHS PFHS PFHS
In le t File
pfbsl pfbsl pfbsl pfbsl pfbsl pfbsl
B o ttie
42 43 44 45 46 47
tmkhMM. Tuning Method Report
sM
.
-
Method:
C :\MASSLYNX\AMELIA-RATS. PRO\ACQUDB\PFOS
Printed:
Wed Jun 09 15:01:35 1999
BACK TO MAIN
Page 1
MS
SOURCE ( ESP- )
Capillary
Cone
Extractor
RF Lens
'
Source Block Temp.
Desolvation Temp.
Set Rdbk
3.00 15 5 0.2 150 250
2.98 -60 -5
150 251
Pressures
Analyser Vacuum
Gas Cell
'
Rdbk
1.4e-4 4.9e-3
MSI
LM Resolution HM Resolution Ion Energy Ion Energy Ramp Lens 6 Multiplier
, .*X
MS2
LM Resolution HM Resolution Collision Ion Energy Ion Energy Ramp Lens 8 Lens 9 Multiplier
Set Rdbk
15.0 15.0 2.0 0.0 5 650
i?
6
Set Rdbk
15.0 15.0 30 2.0 0.0 40 0 650
47
37 44
h m U (k b b W ii S A tj ( e / M j t f
Scanning Method Report
Method:
C:\MRSSLTNX\AMZI.ZA-R1XS.pnoVCOUDB\ -ma
last Modifind: wad Jun 0* 14:17:17 IMS
Frintad:
Wad Jun 0* 15:01:0 1599
advent Dalay { mina ) :
0.00
yenetion : 1 M M of 2 Maaa Paira (lap- )
Intar Channel Dalay ( Sacs ) * 0.03
Span-( Daltons ) :
0.00
Start TIsm (Mina ) >
0.00
End Tlaw (Mina ) :
13.50
Repeats :
1
Channel Parent Daughter Duali (Sacs) Coll Enargy (VI Co&a ( V 1
1 299.00 80.00 0.40 2 299.00 99.00 0.40
45 45
60 60
nwotiosa t 2 M M o f 2 Mua Paira ( ESP- )
Inter Channel Dalay ( Secs ) : 0.03
Span { Daltons ) i .
0.00
Start Tina (Mins ) :
0.00
*
End Tiae (Mins ) :
13.50
Rapaste :
1
Channel Parent Daughter Duali (Sacs) Coll Energy (V)
Orna ( V )
1 399.00 00.00 0.40 * 45 .
2 399.00 99.00 0.40
45
60 <0
(s/ BACK TO MAIN
Paga 1
r / \ u ; UAIAW.V1JLW auiviiVIAKY FORM
BACK TO MAIN
Oste:
(,/l/3
ICO
Am U 062499: quwtiatad ind prlnfead by boj 9/10/99
A A A A A A a M WU> m U A U l U U l U I A A A * * A * A A A U U U U U U U U U W M N M M M N M M M M H H H M H H H H H H A S -U M H O U B 'J A A A U M W O U A > )A A * U K lH O W > lA U * U N H O a > J A W * U M H O W tt^ A W * U N H O U > J llU I * U M M | OOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOS
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Quantify Ceavound m m m m r y Eaport
toxl20,
and p(ha
' '
Saagila Uati C:\IASnnec\aMlla-iata.MO\daaplan\OCOtMa
Last aodlflad: Thu Jun 10 07:03:0 19*9
Hathod:
C :\HassLTKX\anaUa-rata.T0\MathOS\0*Ot9la
Laat odlfiod: Thu JUn 10 04:47:27 1 M
Job Coda:
Frlntad:
Thu Jun 10 07:13:33 1**
Ccapeund 2: I M
1 Mann
anplallat Tact
.
1 060999001
athanol blank
2 060999002
athnnol blank
3 060999003
toxl20, rbl02229>B2Cblk-l
4 060999004
toxl20, rbl02229-Llvarblk-l .
5 060999005
toxl20, rbl02229*5ppb"l
6 060999006
t0x120, rb!02229>10ppb-l
7 060999007
toxl20, rbl02229>25ppb-l
$ 060999008
toxl20, rbl02229*50ppb-l
9 060999009
toxl20, rbl02229~100ppb~l
10 060999010
texl20, rbl02229-250ppb>l
11 060999011
toxl20, rbl02229>SOOppb-l
13 060999012
toxl20, rbl02229-75Oppb-l
.
13 060999013
COX120, rbl02229-1000ppb-l
14 060999014 . nathanol blank
15 060999015
toxl20, 10tr-Cl-250ppb-m, 2/22/9
I 10 060999016 17 060999017 11 060999019 19 060999019
toxl20, 106F-d-250ppb-ad, 2/22/99 texl20, 3K-l-250ppb-aa, 4/19/99 toxl20, 3K-<31-2S0ppb-uad, 4/19/99 toxl20. rbl02229-25Oppb-ccl
20 060999020
nathanol blank
,
21 060999021
tOX120, 153r-64. 2/22/99 "
22 060999022
toxl20, 154r-C4, 2/22/99
I 23 060999023 24 060999024 25 060999025 26 060999026
eoxi20, issr-c4, 2/22/99 tOX120, rbl02229-25Qppb-cc2
nathanol blank toxl20, lS7r-G4, 2/22/99
27 060999027
texl20, 1SSIM4, 2/22/99
28 060999028
toxl20, 159C-G4, 2/22/99 '
29 060999029
toxl20, rbl02229-250ppb-cc3
30 060999030
nathanol blank
1 31 060999031 * toxl20, 57M-G4, 4/19/99
32 060999032
toxlZO, 58M-G4, 4/19/99
33 060999033
tOX120, 59M-G4, 4/19/99
34 060999034 * toxl20, rbl02229-250ppb-cc4
35 060999035
nathanol blank
36 060999036
toxl20, (1M-G4. 4/19/99
37 060999037
toxl20, 62M-G4, 4/19/99
38 060999038
toxlZO, (3M-G4, 4/19/99
39 060999039
toxl20, rbl02229-250ppb-ee5
40 060999040
nathanol blank
41 060999041
tox!20, 149F-GS, 2/22/99
1
42 060999042
toxl20, 170r-Ci, 2/22/99
I1I
43 060999043 44 060999044
tox!20, 17lr-GS, 2/22/99 toxl20, rbl02229-250ppb-ce(
1 45 060999045 . nathanol blank '
46 060999046
toxl20, 173F-G5, 2/22/99
47 060999047
toxl20, 174F-6S, 2/22/99
48 060999048
toxl20, 17Sr-fiS, 2/22/99
'
49 060999049
toxl20, rbl02229-2S0ppb-cc7
1 50 060999050 51 060999051 52 060999052
nathanol blank
.
toxl20, 73M-GS, d250, 4/19/99
toxl20, 74M-GS, d250, 4/19/99
53 060999053
toxl20, 7SM-GS, d2S0, 4/19/99
54 060999054
toxl20, rbl02229-250ppb-ccl
I 55 060999055 56 060999056 57 060999057 58 060999058
nathanol blank
toX12Q, rb!02229-B20blk-2 toxl20, rbl02229-U*orblk-2 toxlZO, rbl02229-5ppb-2
.
59 060999059
toxl20, rbl02229-10ppb-2
(0 0(099(0(0
toxl20, rbl02229-29ppb-2
61 060999061
toxl20, rbi02229-50ppb-2
62 060999062
toxl20, rbl02229-100ppb-2
1 3 060999063 4 060999064
toxl20, rbl02225-250ppb-2 toxl20, rbl02229-S00ppb-2
65 060999065
toxl20, rbl02229-750ppb*2
66 060999066
tox!20, rbl02229-1000ppb-2
I
BACK TO MAIN
hgi 2
*yp Analyta
ltd Cono
Analyta
Analyta
Analyta
Standard
5.99
Standard
12.00
Standard
29.90
Standard
59.90
Standard
120.00
Standard
299.00
Standard
599.00
Standard
898.00
Standard 1198.00
Analyta
Analyta
Analyta
Analyta
.Analyta
QC . 299.b0
Analyta .
Analyta
Analyta
Analyta
QC 299.00
Analyta
Analyta
Analyta
Analyta
QC . 299.00
Analyta
Analyta
Analyta
Analyta
QC 299.00
Analyta
Analyta
Analyta
Analyta
QC 299.00
Analyta
Analyta
Analyta
Analyta
QC 299.00
Analyta
Analyta
Analyta
Analyta
QC 299.00
Analyta
Analyta
Analyta
Analyta
QC 299.00
Analyta
Analyta Analyta
Standard
5.99
Standard Standard
12.00 29.90
Standard
59.90
Standard
120.00
Standard
299.00
Standard
599.00
Standard Standard
198.00 1198.00
XT
9.010 9.038 9.165 9.151 9.092 9.047 9.045 9.024 9.038 9.043
9.055 9.026 9.038 9.043 9.049
9.051
9.021
9.057 9.032 9.031 9.041
9.058 9.031 9.052 9.068
9.074 9.050 9.068 9.061
9.042 9.065 9.060 9.069
9.055 9.072 9.053 9.084
9.099 9.060 9.101 9.082 9.069 9.064 9.096
A n a Cane. flag. IDov
10 52
23 32 (1 122 313 57( 915 11(9
10.99 bb
55.63 bbX
24.34 bbX 34.(3 bb (5.12 bb 130.54 bb 33t.5( bb (11.51 Ml 9(2.(1 bb 1277.01 bb
245 2(3.40 bb 271 291.19 fab 329 353.29 bb 3(4 391.19 bb 299 320.77 bb
". 'A
829 103
15 9 9
13 3 9 7
7
2(4 203.24 bb ` -5
340 3(4.13 bb
22
*2 '7.11 bb 11( 124.24 bb 1(2 173.(7 Ml 2(1 301.(2 bb
1
37 40.10 bb (2 (4.04 MM (7 71.93 bb 2(9 299.43 bb
-3
215 305.44 MM 230 244.44 bb 251 2(9.10 bb 250 2(8.60 bb.
10
41 26 49 24(
1034 002 (4 223
43.75 MM 28.12 bb 51.37 bb
2(3.(( bb ` 1109.17 bb (1.06 bb . 949.1 bb s 239.59 bb
-12
v* -20
. \.
.
30 S( 112 < 241 X 479
. 741 1079
32.40 bb 59.14 bb 120.41 bb 210.16 bb 514.12 bb 795.99 bb 1157.( bb
8
0 0 -6 -14 -11 -3
- Inaila 0(2494: quantlatad and printed by bai (/10/t
I
fluaatliy Calibratiod Waport tarilo, and pflia
'
Calibratioo: C:\Mmmnt\MiaHa-aata.7llolVcaim i\00>Ha
tHt BOdiriadl ItnJ don 10 07sllll7 1M(
Trlntad!
Thu Jan 10 07iU>23 19*1
BACK TO MAIN
Aaalla 0<349li uantlatad and prlatad by fcoj f/10/lt
