Document Ne8q8ezMGnQLj5MJB8B5LLNow

mm f m . AR226-2973 TRADE 3ECHET Study Title STATIC, ACUTE, 48-HOUR EC50 OF H-19,517 TO Daphnia magna Data Requirement Organisation fo r Economic Co-Operation and Development (OECD) Guideline for Testing Chemicals: 202 Author Kevin N. Baer, Ph.D. study Completed On June 9, 1992 Performing Laboratory E. I. du Pont de Nemours and Company Haskell Laboratory for Toxicology and Industrial Medicine Elkton Road, Post Office Box 50 Newark, Delaware 19714 Laboratory Project ID Haskell Laboratory Report No. 423-92 Company Sanitized. Does not contain TSCA CBI Page 1 o f 17 Da Poni HLR 423-92 GOOD LABORATORY PRACTICE STATEMENT This study was conducted according to EPA FIFRA Good Laboratory Practice ( C TP M ^O E C O Prtndplas of Good L a b o r^ P ra rtR ... (C(81)30(FinaJ), Anne 2). and WAFF Jap G oodlatoor^iiyPf^fooitondap^^ 59 NohSan Mo. 3850). SolubBty and stability of ttwja s t sutetM ce J?j * * S waler and under storage conditions u not d e t e m n ^ C ^ ^ o r e ot tn e tM t substance in dilution water were not measured. Protocol am endm ents^w w or& ur H alations nnntmr in Appendix I. Areas of noncompliance (if any) are also documented in the study records. No deviations existed that affected the scientific validity of the study. Submitter: E. I. du Pont de Nemours and Company Sponsor: STUDY DIRECTOR: Du Pont Chemicals E. I. du Pont de Nemours and Company . Jvn N. Baer. Ph.D. miolAeXar Research Toxicologist Environmental Sciences Company Sanitized. Does not contain ISC CB1 3 Da Poni HLR 423-92 TABLE OF CONTEMTS Specific Country Requirements. Good Laboratory Practice Statement. General Information, Quality Assurance Documentation. I. Purpose...................... ............. II. Records.................................. Hi. Materials and M ethods........ A Test Substance.............. B Dilution W ater................. C Animal Culture................. D. Test M ethods......... ........ E Chemical Analysis......... F. Statistical Analysis............ IV. Results and Discussion...... V. Conclusion........ .................. VI. Literature Cited...................... VII. T-' 1' 1'5 .3 11 11 12 13 VIII. Appendix I. Protocol Amendments and SOP Deviations 17 17 Company Sanitized. Does not contam TSCA CBI 4 Da Pons HLR 423-92 GENERAL FORM ATION Sn-Life Study jpiMmMQmsMssL E. I. du Pont de Nemours and Company Wilmington, Delaware 19898 5-1S-S2 / 8-9-S2 5 -1 9 -9 2 /5 -2 1 -9 2 .Company Sanitized. Does tV3to3nta?a TSC&CBi Du Pont HLR 423-92 STATIC. ACUTE. 48-HOUB EC50 OF H-19.S17 TO Daphnia magna SUMMARY oHld-,)9in.51a7n wunaase"rated,static, 48-h ^ s te at ^om i b. f f Qn nomina| concentration,,s " - 1 0 , 0 1 5 m 9 /L Work Aquatic Technician THIS REPORT IS APPROVED F<^R ISSUE. J Kevin N.-Baer, PrTb. Mo/Day/Year Research Toxicologist Environmental Sciences m eaLU m s not contain TSCACB! 6 Du Pont HLR 423-92 q u a l it y a s s u r a n c e d o c u m e n t a t io n Rntffi n f lnsnectiQn: Conduct - 5/19/92 Records, Report(s) - 6/5-6.92 F i^ n g ft sported to: Study Director - 6/6/92 Management - 6/9/92 PitReported by: LlC o K (? - ^ Deboran A. Vifek Quality Assurance Auditor bhfe: Date Company Sanitized. Bees noteosilafe TSCACat 7 Du Pont HLR 423-92 I. PURPOSE Th goal of this study was to assess the acute toxicity of H-19,517 to Daphnia magna neonates less than 24 hours old. II. RECORDS All data and the final report will be maintained either at H ^te llL a b o rm o ry fo rT o x to io g y and Industrial Medicine (Newark. Delaware) or be archived at the Du Pont Records Management Center (Wilmington, Delaware). III. MATERIALS AND METHODS A. Test Substance Purltv anH composition of the test substance are described under General information. A solution of each test concentration was prepared by pipetting the appropnate amount of H-19,517 and directly adding it into dilution water for immediate use. The stability and solubility of H-19.517 in dilution water (see below) and under storage conditions were not conducted. B. Dilution W ater W ater (Table 1) originated from the Haskell Laboratory well and flowed through ^ u 'a ria conmining fa.head minnows, Pimephales promelas, prior to uso ,n thrs daphnia test. C . Anim al Culture Parent Daphnia magna were reared at Haskell Laboratory in 250 mL Pyrex Ita k e rs (1 per beaker) which contained 200 mL of filtered dilution water or a u to ^a v e d ^ 1^ dilution water held at approximately 20C. Dapt. dsw arefed two a l f l ^ ^ * j - Qf dilution water renewal process. N a n te s 5* S n ^ ^ n r o n ^ ^ S identified by labels on the beakers and test vessels. wero D . Test Methods [11 Six test concentrations and a water control were used. Nominal test concentrations were 3.0,6.25,12.5, 25.0, 50.0, and 100 mg/L. Pyrex beakers (250 mL; 7-cm diameter) containing 200 mL of test depth) were used. Each replicate (four per concentration) contained five daphrads CBICompany Sanitized. Does not contain TSCA 8 Du Pont H LR 423-92 transitionglight(appTO rriately ^L ux^p rw ^d'rvg^a ^^ (imm^ , Bity) w a s ^ e inability to swim at least two body lengths in any one direction wrthm 15 seconds after gentle prodding with a glass rod. Dissolved oxygen (YS! Model 54A, Yellow Springs ^ ^ L ^ M A V ^ d 98' H>' pH (Coming Model 610^ ^ 9 ^ c l ^ m e j ^ M e d f l d d ^ ) ^ s s s s -s w i-- Sitt&THa^Chem^cSw -^ftjS^g S p rn ^ n ^ ^ of ih y liS io n water were measured before daphnids were added at the beginning of the test. E . Chem ical Analysis Concentrations of H-19,517 in the test solutions were not conducted. =. S tatistical Analysis The 24 and 48-hour EC50 and their associated 95% fiducial limits were calculated by the probit method {2]. IV. RESULTS AND DIS'CUSSION Two ranoefinding studies were conducted. The first rangefinder was not covered to prevent possible volatilization of the co m ^n e n ts an was ^ concentration, rangefinding study was covered and c o n d u c t five unfed daphnids per replicate, exposed and 100%, respectively, test. All chemical and physical parameters (Tables 2 and 3) In the definitive test were within expected ranges. Company Sanitized. Does not contain TSCACB1 9 Du Pont HLR 423-92 SS52 10 to 15 mg/L (Table 5). V. C O N C LU S IO N 5%<* nfktence intervai * H-19.517 was moderately toxic to Daphnia magna neonates (less than 24 hours ole: in an unaerated, static, 48-hour te s t. VI. LITERATUR E CITED Organisation to r Economic Co-O peraton and Development (OECD). Guideline`or Testing Chemicals: 202. 4 Apnl 1984. 2 The Probi. Procedure. 1985. SAS User's Guide: S tatistics. Version 5 Edition SAS Institute Inc., Cary, NC. Company Sanitized. Does not contain TSCACfii 10 Du Pont HLR 423-92 STATIC. ACUTE, 48-HOUR EC50 OF H-19,517 TO Daphnia magna TABLE 1 Chemical Characteristics of Haskell Laboratory Well Water1 P ara m ete r Concentration Parameter Concentration BOD. mg/L COD, mg/L DOC, mg/L TOC2, mg/L Total Kjeldahl N, mg/L Ammonia N, mg/L Turbidity, NTU Phenolics, mg/L Color, Co/Pt S olids total suspended, mg/L Aluminum, mg/L Antimony, mg/L Arsenic, mg/L Beryllium, mg/L Cadmium, mg/L Calcium, mg/L C hloride3' mg/L Chromium, mg/L Cobalt, mg/L Copper, mg/L Cyanide. mg/L Iron3, mg/L Fluoride3 <2 <10 2.8 <0.5 <0.2 <0.05 1.6 <0.050 <5 <4 <0.100 <0.060 <0.010 <0.001 <0.002 24.5 6.2 <0.010 <0.020 <0.010 <0.025 0.025 <0.1 Lead, mg/L Magnesium, mg/L MBAS/LAS, mg/L Mercury, mg/L Nickel, mg/L Nitrite3, mg/L Nitrate3, mg/L Phosphate3, mg/L Potassium, mg/L Selenium, mg/L Silver, mg/L Sodium, mg/L Sulfate3, mg/L Sulfide, mg/L Thallium, mg/L Zinc4, mg/L Volatile priority pollutants Acid extractablei priority pollutants Base/neutral priority pollutants Pesticides/PCBs Organophosphate pesticides5, pg/L <0.075 3.8 <0.05 <0.00020 <0.020 <0.1 <02..11 1.8 <<00..001005 8.0 4.8 <0.05 <0.01 <0.020 NDe ND5 NDS ND6 <0.50 1Date of sample collection 30 December 1991 unless indicated otherwise, analyses performed at Environmental Testing and Certification Corporation, Edison, New Jersey; 2Date of sample collection 15 January 1992, performed at Du Pont Engineering Test Center, Newark. Delaware; 3Date of analyses 13 January 1992, analyses performed at Du Pont Chemicals, Jackson Laboratory, Deepwater, New Jersey; Below the Published Method Detection Limit (BMDL); sAnalyses performed at Hazleton Laboratories, Inc., Madison, Wl; 6None detected. Com pany Sanitized. Doe* . oes nnl nof .. contain TSCA C8! Du Pool HLR 423-92 STATIC, ACUTE, 48-HOUR EC50 OF H-19,517 TO Daphnia magna TABLE 2 Water Chemistry ot the Water Control at Test Start Total Alkalinity (mg/L as CaC3) 76 EDTA Hardness (mg 1 as CaCOz) 75 Conductivity (ixmhos/cm) 172 notCompany Sanitized Does contain TSCA CbI 12 N om inal Concentrations (mg/L) Aa Ba Ca Da Aa Ba Ca Da Dissolved Oxvaen. mg/L H20 Control 3*0 6 l> 5 12.5 25.0 50.0 100 8.9 8.8 8.8 8.9 8.8 8.8 8.9 8.8 8.8 8.9 8.8 8.8 8.9 8.9 8.8 8.9 8.8 8.8 8.8 8.8 8.8 8.8 8.8 8.8 8.9 8.9 8.8 8.9 m H20 Control 3.0 6.25 12.5 25.0 50.0 100 7.5 7.5 7.5 7.5 7.6 7.5 7.6 7.5 7.7 7.7 7.7 7.7 7.6 7.6 7.6 7.6 7.6 7.6 7.6 7.6 7.6 7.6 7.6 7.5 7.6 7.5 7.5 7.6 8.9 8.9 8.9 8.9 8.8 8.8 8.8 8.8 8.9 8.9 8.8 8.8 8.9 8.9 8.9 8.9 8.8 8.8 8.8 8.8 8.8 8.8 8.9 8.9 8.8 8.8 8.8 8.8 7.9 7.9 7.9 7.9 7.9 7.9 7.9 7.9 7.9 7.9 7.9 7.9 7.9 7.9 7.9 7.9 7.9 7.9 7.9 7.9 7.9 7.9 7.9 7.9 7.9 7.9 7.9 7.9 aA, B, C, and D represent replicate test vessels containing five daphnids each at test start. CompanySanltizod_^ ^ ^ ^^ 13 Du Pont HLR 423-92 STATIC. ACUTE. 48-HOUR EC50 OF . nanhnia maona TABLE 3 Continued Physical and Chemical Characteristics of H-19,517 r ^ Test Solutions N om inal Concentrations (mg/L) S ta rt Ba Ca Da 48 Hours Aa Ba Ca Da Is i fim tu is ^ C H2O Control 3.0 6.25 12.5 25.0 50.0 100 19-9 19.9 19.9 19.9 19.9 19.9 19-9 19.9 19.9 19.9 19.9 19.9 19.9 19.9 19.9 19.9 19.9 19.9 19.9 19.9 19-9 199 19.9 19.9 19.9 19.9 19.9 19.9 19.9 19.8 19.8 19.9 19.9 19.9 19.9 19.9 19.8 19.8 19.9 19.9 19.9 19.9 19.9 19.8 19.8 19.9 19.9 19.9 19.9 19.9 19.8 19.8 19.9 19.9 19.9 19.9 represent replicate test vessels containing live daphnids each at test aA, B, C, and D start. CompanySanitized. Does norcontain TSCACBt 14 Du Pout HLR 423-92 STATIC, ACUTE, 48-HOUR EC50 OF H-19,517 TO Daphnia magna TABLE 4 Observed Immobility of Daphnia maana Exposed to H-19,517 __ i ao in a Qta*r> IInaerated Test Observed Immobility in Percent Nom inal C o n c e n tra tio n s (mg/L) Aa W ater Control 3.0 6.25 12.5 25.0 50.0 100 0 0 0 0 40 60 80 Start Ba Ca Da 0 0 0 0 0 0 0 0 0 1000 20 0 0 80 100 0 0 60 80 60 48 Hours Aa Ba Ca Da 0 00 0 0 00 0 0 00 0 80 100 110000 20 16000 100 100 100 100 100 11160000000 aA, B, C and D represent replicate test vessels containing live daphnids each at test start. 15 i^paij/ Sanitized. Does not contain TSCACBI Du Pont HLR 423-92 Tim e STATIC, ACUTE, 48-HOUR EC50 OF H-19,517 TO Daphnia magna TABLE 5 Nominal H-19,517 Concentrations Causing Immobility to 50% (EC50) of Daphnia magna at Specific Time Intervals EC50 (m g/La) 95% Fiducial Limits (mg/La) Slope Y-intercept i ;{ : 2 4 U fiU fS 4 8 H ours 46 12 16 200 10 15 3.2 5.5 -5.3 ; -6.0 aBased upon nominal concentrations. Coes not contain tsca < 16 p -w V , Du Pont HLR 423*92 STATIC. ACUTE. 48-HOUR EC50 OF h-19,517 TO Daphnia magna APPENDIX I p r o t o c o l a m e n d m e n t s a n d s o p d e v ia t io n s There were a total of 6 Protocol deviations and 4 SOP deviations. ftrnndments 1. Daphnid cultures will be feo green algae; species and rate will be at the discretion o f the Study D rector. ^ e n n fn r r h a n o e : Agae is food of choice; SOP in progress. 2. Water samples for analys s will not be taken. Pftflann for change: Ssensor request. 3 All test concentrations we-e covered with glass plates to prevent potential volatilization of the compounds in the formulation. fo r change: Socnsor request J 4. Synonyms: change t fo r chance: E-o r in protocol. 5. AQ019-E and AQ027-P ere supporting SOP's. n ^ - f c r r i i a n o a : Testing SOP in error: revision in progress. 6. AQ023-P and AQ026-P ere not filed in QA at the time of this study. Rflasnp fo r change: De ay in issuing SOP s. SQP deviations !. AQ009-E has AQ011-E -stead of AQ-037-P reference. 2. Culture water bath temperature and hatched date were not recorded. 3 The batch number for beaners were not documented in the study notebook; thus 3- TM c K o n in g o l the vessels could not be venfied. 4 The date, time, and temperature of when the waterbath was filled was not recorded in the study records. 1 7 0`Wsijotco,,(aJJTscAoa,