Document MvreO8107BJRO5BvjEO2QbZz

AR226-0535 ACUTE TOXICITY TO AQUATIC INVERTEBRATES (E.G..DAPHNIA) TEST SUBSTANCE Identity: aPlesrfolbuoeroroecfteyrlrseudltfoonaasteF,ludoirdoeaclkyylldiammemtohnyliaummmodneriiuvmatisvalet.; may [1- D1,e1c,a2m2i,n3,i3u4m,,4,N5-,d5e,c6y,l6-,N7,,N7,-8d,i8me8t-hhyelp-t,adsealctafwlituhoro-1- octanesulfonic acid (1:1), CAS # 251099-16-8] LR-e1m4a3r9k4s:referTrehdet3o Mbyprtohdeutcetsitolnablootrantuomrbyears was Lot P3025. 1. The test sample The sample was is ilnabwealteedrF(-a1p1p6r1o5x,imLaottel1y. 3T0h-e40te%sttsesatmspulbestisanacem,ix6tu0r-e70of%twhaeteters,tasnudbs0t-a5n%ce of residual perfluorochemicals). The test sample appears to be a A2ll-pvhaalsueesdirseppeorrstieodnr(ecllaeteartloiqtuhiids mixture. with `opaque solid) were made to which adjust rapidly for the saectpuaarlatceosncaefntterraatgiiotnatoifont.heNtoesctaslcuublsattainoncse in the test sample. METHOD Method: OECD 202 Test type: Static acute GLP: Year CNomopleted: 1936 `SApneacliyetisc:alDamopnhintioarimnagg:naDO, pH, temperature and conductivity were monitored daly. Statistical methods: EL, values calculated using Trimmed Spearman- Karber method. NOEL value calculated using Steel's Many-One Rank test. Test daphnid source: Obtained from cultures maintained by AScl TCoersptordaatpihonn,idDualguteh,atMsNt.udy initiation: <24-hours Test conditions Dilution water: Dechlorinated City of Duluth, MN tap water. Water was aerated for 24-hours prior to use in the test. Dilutiohnawradtneerssc:hemis4t4rym:g/L as CaCOs PH: 8.04 04243 Lighting: Cool-white fluorescent bulbs. Photoperiod of 16-hours light, 8-hours dark. No transition period noted. Sfrtaoctcikonasn. dTetsetstsosloultuitoinosnwseprreepparreaptairoend:inWdiavtiedura-lalyccfoormemaocdhated cinon1ceLntofradtiiloutniobnywmataesrs. aTddhietisoonluotfivonisgowreoruselyvisghoarokuesnlytessttirsruedbsftoran2c3ehsoiuprhson(evdorftreoxm1/t2hetove1s/s3elsoaltutmiiond-ddeepptthh).afTtehreseatqtulienogufsorp1h-ahosuer.was 2E0x0pomsLuorfetevsetssseollsut:io2n5.0 TmhLebsoorloustiiloincsatweegrleasksepbteackoevresrecdondtuariinnigng the test. Number of replicates: Four Number of daphnids per replicate: Five Number of concentrations: five plus a negative control Water cDihsesmoilsvterdy oduxryignegntrhaensgteud(y0:- 48 hours): 86-91 mg/L (control exposure) 8.0-88mglL (50 mg/L exposure) pH range (0 - 48 hours) 8.04 -8.11 (control exposure) 7.92-800 (50 mglL exposure) Test temperature range (0 - 48 hours) 209-21.0C Conductivity range (0 - 48 hours): 142-155 pmhos/cm (control exposure) 120 -- 124 pmhosfem (50 mg/L exposure) Element basis: mortality and immobilization RESULTS Nominal loading concentrations: Bk control, 3.13, 6.25, 12.5, 25, 50 mg/L Element value: 24-hour EL = 27.0 (18.7-39.0) mg/L. 48-hour ELsy = 11.3 (9.6-13.2) mg/L. 48-hour NOEL = 6.25 mg/L All element values based on nominal concentrations. Statistical Evaluation: The ELso values and 95% confidence intervals were calculated by the Trimmed Spearman-Karber method. The NOEL was calculated using Steel's Many-One Rank test using the TOXSTAT statistical software Version 3.2, University of Wyoming. 04244 Biological observations: Daphnids in the negative control, and the 3.13 and 6.25 mg/L treatments appeared healthy `and normal throughout the: test with no mortality, immobility or overt clinical signs of toxicity. The effects noted in this study were mortality; no immobilization was noted at any test concentration. The number of surviving organisms were determined visually and recorded initially and after 24 and 48 hours. E E EPp E `Cumulative percent mortality: pe I Control response: satisfactory Remarks: Values reported are for the test sample. No calculations were sample. made to adjust for the concentration of the test substance in the test CONCLUSIONS The test substance 48-hour EL50 for Daphnia magna was determined to be 11.3 mg/L with a 95% confidence intervalof 9.6-13.2 mg/L. The 48hour no observed effect level (NOEL) was 6.25 mg/L. Submitter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY Reliability: Klimisch ranking 2. The study lacks analytical measurement of test substance concentrations in the test solutions and sample purity is not sufficiently characterized. Additionally, data is foar mixture and toxicity cannot be positively attributed to didecyldimethylammonium Perflucrooctylsulfonate salt alone. REFERENCES C04245 `This study was conducted at AScl Corporation, Environmental Testing Division, Duluth, MN, at the request of the 3M Company. OTHER Last changed: 5/24/00 04246 EAnSierloCnormpeonraalioTnx/AiSnegl-DiDvuilustohn Ase Stdy 104 010068 STUDY TITLE ACUTE TOXICITY OF #5 2s DEVELOPMENTAL MATERIAL TO DAPHNIA MAGNA DATA STANDARD `OECD GUIDELINE 202 STUDYCOMPLETED January 10, 1997 TESTING FACILITY AScl Corporation/AScl-Duluth Environmental Testing Division 4444 Airpark Boulevard Duluth, MN 55811 TFealx. NNoo.. ((221188)) 772222--24509420 STUDYIDENTIFICATION NUMBERS AScl Study IDF 5010-068 3M Company Lab Request# P3025 SSppoanmsmoerr:St3uMdyCIoDmpPa3n0y25 1 042A? EAnSveilrCooormpeonranlioTne/xAiSneglDDiuvliuothn "Ase Sty 101 5010-068 1.0 GENERAL INFORMATION | study Tite {| sponsor f | OECD Guideline 202 Acute Toxicity of P2035 Developmental Material to Daphnia magna. 3M Environmental Technology and Safety | Services, Building 2-3E-09, 935 Bush Avenue, St. Paul, MN 55133. | sponsor's Representative I| i Susan A. Beach, 3M Environmental T3eEc-0h9n,ol9o3g5y BaunsdhSaAfveetnyueSe,rvSitc.esP,aulB,uiMldNing5521-33; Tel No. (612) 778-7452. | Sponsor's Lab Request# P3025. | Testing Facility |l Principar l investigo ator pepooi mmnoor--| Testing Facility Director AScl Corporation/AScl-Duluth Environmental Testing Division, 4444 Airpark Boulevard, Duluth, MN 55811; Tel. No. (218) 722-4040. Donald Mount. | Definitive Test Dates Data Validation and Report Review December 11-13, 1996. | `Alan Mozol. ron Signare: | | Retention of Raw Data and Final Report l Location of Raw Data and Final Report i l "Two years from the date study is completed. AScl Corporation/AScl-Duluth Environmental `Testing Division, 4444 Airpark Boulevard, Duluth, MN 55811; Tel. No. (218) 722-4040. SSppoonnssoorr: Su3dMyCIoDmVpPa3n0y25 2 C24248 EAnSveitrCoonmrepnotraaltiToenstAinSgelD-iDviuslitann 5c Study 104 5010-068 2.0 OBJECTIVE(S) To determine the 48-h EL, (effective loading) and 48-h NOEL (no observed effect loading) of | the test substance, L-14394 Developmental Material, for Daphnia magna. I I `GTuhiidsesltiundey2w0a3s(cOonEdCuDcte1d993a)c.corTdhiengtetsot tshoeluAtSieonlsswteurdey pwlaatnerr-eatacicnoemdmboydattheedSpfroancstoiornsan(dWAOFE)CD prepared following Girling, Whale, & Adema 1994. 3.0 TEST METHODS | Test Substance | | | Test Organisms : | I| Dilution Water | Properties: #335 two-phase liquid before agitated, light colored, nearly odorless slurry after agitation, specific gravity ca. 1.2 g/cc, and insoluble in water. { Test loadings: 0 (dilution water control), 3.13 mg/L, 6.25 mg/L, 12.5 | imngd/iLv,idu2a5llmygf/oLr,eaacnhd c5o0ncmegn/trLa.tioTne.stTlhoeadisonlgutsioolnustiwoensreweprreepparreepdarbeydmass addition of vigorously shaken test substance in 1 L of dilution water. The solutions were vigorously stirred (vortex 1/3 to 1/2 solution depth) for 23 h._The aqueous phase was siphoned from the vessel at mid depth. | Source: Healthy, less than 24-h old Daphnia magna neonates obiained from AScI stock cultures maintained in water of test dilution water quality. Use in test: Gravid adults isolated in dilution water 24 h before the test iHnaintidalteiodn.witUhseads5mooortghanbiosrmse/speippelticwaittehaannd i4nsriedpelidciataemse/tteesrtocfon6cemnmt.ratDioind. not feed during the test. | Source: Dechlorinated Cityof Duluth, MN, tap water. The most recent | chemical analysis is in Raw Data Package. | CChaarCaOct)eraisntdicas:pHoAtftest8.0t4.imeA,erthaetewdatfeorr h2a4dhaphraiorrdnteossusoifng44inmtgh/eLtes(ta.s 1 SSppoonosro:r S3yM CIoDmYpaFn0y25 3 0429 ExCphoasmbuerres | l Observations || | { CEanldcpuolianttions | EAnSveilrACosonrempeoSnrutndiToyen/s1DAiSnegl0D1Di0uv.li0ui6toh8n Type: 250-ml borosilicate glass beakers. Use test, in test: 200 ml of dilution kept covered, except when water or test experimental sooblsuetrivoant/icohnasmbweerr.e During made. the Duration: 48 h. Dfaluiolryespcheonttopbeurlibos.d: 16 h light and 8 h dark, maintained using cool-white Temperature: 21C. Biimomloobgiilcailz:edDaanidlyd-eagdenttelsty oarggitaantiesdmsthe(iemxmpoobsiulriezecdhaomrbgaenrissamsndwethreen tchoousneted atghiattatdeidd).notDisdwinmotwirtehmionve15anseycdoanpdhsnaifdtserferxopmostuhreeexcphoasmubreerschwaemrbeergsenattly 24h. ||| dWaaitlye-r tcehmepmeirstartyu:re,(1p)H,AtDtOes,t iannidticatoinodnu-cttiovtailtyh.ardAnlelsdseatenrdmialnkaatliionnisty,we(r2e) | made according to APHA methods (1995). ELgy Trimmed Spearman-Karber method. NOEL: Steel's Many-One Rank Test (TOXSTAT statistical software, Version 3.2, University of Wyoming). | SSppoonnssoorr: St3uMdy CIoDmVpaP3n0y25 a 04250 EAnSveilroCnomepnotraalioTne/stAiSncgl-DDiuvliustosn "ASel Study 104 5010-068 3.0 RESULTS `Test Substance Toxicity (Pertinent data are in Table 1) | 24-h ELgy: 27.0 mg/L (18.7-39.0 mg/L). | 48-h ELgg: 11.3 mg/L (9.6-13.2 mg/L). Ranges of Water Chemistry Parameters 48-h NOEL: 6.25 mg/L. | Total hardness (as CaCO; mg/L): 41-44. I | _ _ Alkalinity (as CaCO; mg/L): 42-45. { Temperature (C): 20.9-21.0C. | DO (mg/L): 7.99.1. | PH: 1928.11. Specific conductivity (umhos/cm): 120-155. _ 4.0 TEST QA/QC |f oQra/OQtC crieteeria -- A ee-- 1| |{ Test organism dead, immobilized or trapped in control exposure must not be more than 10%. | 0% dead, immobilized or trapped. I `The lowest DO concentration at test termination mustbe at least 60%ofthe air saturation value at test temperature. `The lowest DO concentration measured was 89% of the air saturation value at 21.0C. Test duration must be 48 h. "The test duration was 48 h. Reference substance toxicity test (this test is | Current control chart for D. magna acute optional). testing is included in the raw data package. SSppoonnssoorr: S3uMdCIoDmApPa2n0y25 s 04251 EAnsveosCnlompeSoartiyToo0nnA0gSScD0u1o30nn08 5.0 REFERENCES American Public Health Association (APHA). 1995. Standard Methods for the Examination of | `Water and Wastewater. APHA, Washington, D.C. | {|| GDAiecrtcleiornmmgmi,onAid.naEgt.e,tdheGF.ArFaq.cutaWitohinacsl.Teo,CxhiaecnmidtoysDpo.hfMe.ProMeo.r2l9Ay(d1We2am)t:ae2.r6-4S15o9-l92u46b.4l9Ae.CGoumidpelleixneMiSxutpuprleesmeUnstinfgorWater- | I| HEasmtiimlattoinn,g MM.eAd.i,anR.LCe.thaRlusCsoon,ceanntdraRt.iVon.sTihnurTsotxoinc.ity19B7i7o.asTsraiysm.meEndviSrpoena.rmSacni.-KTaercbhenrolM.ethod for || 11(7):714-719; Correction 12(4):417 (1978). 1 Organization for Economic Cooperation and Development (OECD). 1993. OECD Guidelines for | `Testing of Chemicals. OECD Publication Information Center, Washington, D.C. | | University of Wyoming, Toxstat Version 3.2. Cheyenne, Wyoming 82001. | SSphoanvsoerr.u3yM C1o5m9p7a5n0y25 . 04252 rt Comrssonn RES TABLE 1. ACUTE TOXICITY OF Pes DEVELOPMENTAL MATERIAL TO DAPHNIA MAGNA | Tees emT g T ewm T e e Cs es T e e e ee) 5 ww wey] -- EFFECT AND NO-EFFECT LOADINGS WITH CORRESPONDING 95% CONFIDENCE INTERVALS C=eoeo, TT ow0eenen| Tewcicooememmnees] Co emm, 1 5 oo T Toaerw] s oSr,n4 cSoeryn 5 04253