Document MaOqkGomqXOzpJ9emM9zp4ba

Toxicological Summary PFOS Dietary Acute Mallard Study Test Substance: Perfluorooctancsulfonate (PFOS) Structure; p1o-tOacstsanieusmuslaflot,niCcASa#cid2,7951-,3192.23.33.44.5.5,6,67,7,88$-heptadecafluoro-, Remarks: sTthoeretdesutndseurbstaamnbcieenits caonwdhiittieonpsoawndderpu(ri3tMy wloats#2o1r7i)g.inalTlyhedestaemrpmlienewdatso tbeechn9i0q.ue4s9.% bIny aLCsu/bMsSe,que'nHt-aHnMaRly,sis"oFf-tNhMeRtesatndsubcslteamnecen,talpuraitnyalywsaess determined to be 86.9%. The nominal and measured dietary PFOS concentrations in this summary are based on the 86.9% purity value. "The inlife phase report was originally completed in 2000, and reported v2a0l0u4es tboaserdefolncttheth9e0.s4u0b%sepquuretnyt esrteivmiastiioonn. inThseamrepploert pwuarsityr.eviseTdhiins Toxicological Summary reflects these from all of the associated reports of acnhaalnygseess oasf wieslslueass cionlclleucdtiendg druesruilntgs the in-life phase. METHODS MAeStThMod:StOanPdParTdSE8S5S07.-282700(1(9D9r7a)ft), FIFRA Subdivision E, Section 71-2, OECD 205 and Type: Dietary Acute ear: 1999 in life test, 2000 for report, 2004 for amended report Species: Mallard (Anas planyriynchos) Test Phases: AExcpcolsiurmeatpei-roi9ondd--ay5sdays Post-exposure recovery period -- 3 or 17 days (Experimental Day 8 or 22, respectively) Test Bird Age: 10 days old at est initiation Number replicates oinf cRaecphlitceasttessu:bsTthaenrcee were six treatment. reTphleirceatewseroef the five cmaolnltaorldstrpeeartmreenptli,caatned(ptewno) {froreaatmteontatl(owfhi3c0hmianlcllaurddesd icnigthhtedcoosnetrgorloutprse)a.tment and 10 mallards in each test substance. 1 GOOSEO "8,Thehaalnfitmhaelsrewpelriceataells efxrpoomsecdacfhortrfeiavetmdeanyts,graonudpt(h3enofhetlhdef6orraeprleiccaotveesryfrpoemriotdh.e ActontDraoyl gcornotuipnuaenddin1 trheeplsitcuadtye ufntriolmDcaaych22d.ose level) were sacrificed while the other half were tFeesetdingapnhdasWeasotefr:theFsotouddy.anTdhewamtaelrlawredrsedipdronvoitdreedceaidvelainbyitufmordmuroifnagntaicbicoltiimcattiroenatmaenndt during either the acclimation of est phase of the study. AmanlallayrtdiclailverMoannidtsoreirnugm:byThreevetresste-spuhbassteanHcPeLcConacnendtmraatsisonspwecatsrodmeettreyr.mined in feed and Sptoaptuilsattiicoanl Mfoerthaodgsi:venLCeSx0pos(Luertehaltimceo)nceanntdratLiToSnOth(aLtetrheaslulttsimien 5th0at% rmeosrutlaslitiyn oSf0a% mortality of a population for a given dose) values were calculated by probit analysis using were the Statistical compared by ADunnanleytsti'ssSytesstt eumsi(nSgAST/OSXTSATT:ATPRsOofCtwPaRrOeBI(DTu)n.netBt,od1y95w5e;igGhutlldeayt,a r1e9g9r0e)s.sioAnls woitthhercsotnacteinsttircaatlieovnasolufatPiFoOnSs wienretiscsouneds.ucted with Excel worksheets including Average Daily Intake (ADI) of PFOS for each treatment group was estimated on a pen basis and did no tke into account potential differences between maleand females. Food consumption and adult mallard body weight exposure period and the ADI was calculated adsatfaolwleorwse (aEvqeuraatgieodn o1v):er the duration of the ADI(pHgEa))==AF AvXeraEeAgerFeeCgdCosnO swuemipgiRhotngEhhDdiU)n)I d),O pyN oe,ipme, PROS) (aEquation) Test Diet Preparation: The test substance was mixed directly into the feed ration by `means ofa Hobart used in this study mixer. were 0 No carrier was (control), 87, used. 17.6, 35N.o1,mi7n0a3l, di1e4t1a,ry28c1o,nc5e6n2t,raatnidons11o2f5PpFaOrSt.s per million (ppm). RESULTS oMfeaPsFuOrSedinDitehte Cfoesntcednicttr.atiTohnes:meWialndlpiefreceInnttemraetcioovnearly Loifd.thdeetmeartmriinxe-sdpickoendcenftoreadtiwoanss 94.7%, while the test fecd samples had measured concentrations of PFOS that ranged <frLo0mQ,929.t4o1,11189.%7,o3f8.t6h,e71n.o5m,i1n6a7l,v2al7u9e,.516M,eaansudre1d14m8epapnm.concentrations of PFOS were: Mortalities and Clinical Observations: A summary of the percent morality for cach rcatmen group i given (Table 1). 2 CRON TPaFbOlSe v1i.aCtuhmeudlieatt.ive percent treatment-related mortality of juvenile mallard exposed to Nomimal . Days Cone. Measured ADI Exposure period Recovery Period (ppm) m_ (mgkgidsy) T23 5165 74 8 To8nw7ol _2L9O.41QT <2L07Q0.00 00 00 00 0000 00 00 00 315716 318867 527400 0 00 00 00 0 0f0 000 00 00 01431 7116S7 262034 00 00 00 00 0000 00 00 00 281 279 7420 0 0 0 00 10 20 20 15622s n51a6 249 900 00 00 200 102[08 390% %30 3%0 soAnvseurmapgteondavlaylemsakfeom(ADDaTy,sm05g. PROS boy Weighvdny) wa calmed wilh body weight and Teed XLiommiotrotfalqiutaienstoactciuornre(dLOinG)anfyorPaFiOmeSnitn vhe deicitntlwhaes e1.5xpteondme.d recovery peiod from Day $10 Day 22. In the control group, no mortalities occurred at any phaseofthe study and all mallards awneyreovneorrtmsailgnisn oafppteoaxricaintcyetahnrdoubgehhoauvtiotrh.e sTthuedryeatwceornecennotrtarteiaotnmsenlte-srseltahtaendomroerqtaulailtiteostohre d1o4s1esppofm2n8o1m,i5n6a2l odrose1.125HopwpemvePrF,OmSoritnaltihteydwita.s noted for mallards treated with nominal aInndthceo2n8t1inupepdmutnrteialtmtehent,afsiicgmnosoonf otofxiDcaityy 8w.ereThoebsseersviegdnspriinorcltuodeddeartuhffslteadrianpgpeaatrDaancye,4 Mreodruacleidtyroeafcmtiaolnlartodssotcicmuulrired(soonunDdayasnd7 maontdio8n,)a,ndlebtyharDgay,y a8nadculmouwleratilviembmowretaaklinteysso.f 20% was observed. Following Day 9, the remaining mallards in the 281 ppm treatment had retumed to a normal appearance and behavior for the durationof the study. Icnontthienu5e6d2upnplmtterremaitnmaetnitongrfoorupt,hossiegnbsirodfs tsoaxcirciiftiycewdeorne Dinaiytia8llaynodbsunetrivletdheonafDtaeymo3onanodf Day 8 for those birds sacrificed on Day 22. Signs of toxicity included a ruffled a`mpopretaarliatnicees,werreeduncoetdedreoanctDiaoynst5o, s6tiamnudli7, floerthaartogtya,l acunmdullaotwievre lmoirmtbalwietaykonfes3s0.%=bSyinDgaley b7.ehRaevmioarinfirnogm mDaalyla9rodfstahpepesatrudeyd. to have recovered and were normal in appearance and iInnctlhueded11r2u5ffplepdmatprpeeaatrmaenncte,grroeudpu,cesdignrseaocftitoonxitcoitsytiwmeulrie, flierstthaorbgsy,erdveepdreosnsiDona,yl2o,ssanodf Dcoaoyrdi7,naaticoun,muplraotsitvraetemoproasltiurtey, odfep9re0s%siowna,s coobnsveurlvseido.ns,FaonldlolwoiwngerDlaiymb7,wenaoknaedsdsi.tionBayl mortality was observed. The one mallard that survived to Day recovered, and its appearance and behavior was normal throughout the remainderof the recovery period. 3 GROSRT Both LCS0s and LTSOs were determined by probit analysis using the morality data (Table 2). The dietary 8day LCS0 for PFOS was 603 ppm with a 95% confidence iBPnoRtdeOrySvawlaeniodgfhp4tr3oa1bnitdtoafno9ao3ld8yscpeopsnmsw.uamspTtchioeonndsudlcaottpeaedwowefirttehhetthhceonnmcsoerenatdlriattiycodnta-mtraa.estpeTonahsveedrcaaugryevedLawiDlaSys0d3o.b6sa5s5eo.df oconnfaivdeernacgeeindtaeirlvyalmoafk1e17(tAoDI20)1wmags P15R0OmSg PboFdOySwAekigbhotddayy.weTghhetdslaoypewoifhth4e d9o5se%. tdaetsap,onwsaescu4r9v7e wdaayss.$47T9h.is TrheseulltasatgrLeTeSd0,witehsticmuamtueldatfirvoemmtohreal1i1t2y5 dpaptam wdhoesreeg2ro0u%p `mortality was observed at day 4 and 9%0 mortality was observed at day 7. Table 2. Estimates of lethal concentration (LCso) and lethal time (LTso)of a population of juvenile mallards in a dietary acute toxicity study with PFOS. * Ey Effect metric Da5sy LC500709(2ppm) 5 as ESTr -- r 251 por m 9Y2C3 1S2625pompom 4oi9s NA mE s moE r spa onder ats cl, 7s92o53s.i%3i0iC3zI9g SSsl2iop0se 451.958 S655 N-- A7 --1255 7a3s8-s55s5s ssiozs oBbosdeyrveWdeigthhtr:ouTgrheoauttmetnetrseuldatyed(Teaffbelcets3)o.n bSotdatyiswtieciaglhlyt saingdnifbiocdayntweriegahttmegntanclwaeircde orfedcuocnttiroonls)oin broedsyerweciognhctewnerarteioobnsseurvreidnignabirDdasyexSpaonsdedcotnotPinFuOiSngaotn28t1hromugg/hkgDa(y4215%. On Day 22, body weight was also significantly reduced in the 146 ppm and higher treatment levels. While there was a statistically significant reduction in body weight in `mallards from the 8.7 ppm treatment for Days and 8, these effects were not considered dtbroersatmguernotupraelwaetteiidg.hedTodhsisesscmoatnyhcilnuhsahivoeencbwoanetsnrobldassueadnd0onoththiheser friacctattltmheainttftfhgeeromnuacpleslaaradnnsdditnhneothGefto8f.e7PnFpeOpeSms efxrpoomsutrhee. 17I.n ad3d5.o1na,dth7er0e wperpemngordouopssortheatawdourleddubceticoonnssiisntebnotdwyitwheiaghdtosien-rmeaslploanrdsse eltonship ` Cras `Table 3. Average body weight (g) ofjuvenile mallards exposed to various dietary concentrationsofPFOS. Treament Body Weight Body Weight Body Weight Body Weight Body Weight (ppm) Dayo* Days Day8 Day 1S Day 22 Control 135224 279+42 41030 64056 823249 87 19:14 226*436 3170466 575+32 773438 176 14614 277426 377432 62542 811481 35.1 147216 275232 375437 620444 828573 703 14330 260%55 34390 579297 7824135 141 14319 242237 331448 56456 688% 86 281 129417 161*28 220*55 467%S1 701d] 562 144223 137027 175*434 304048) 613+ +91 125 147225 1124428 185% 373 634 "TTody weigh s reported as means and standard deviations on awet weight basis. *SSitnatciestnica1l,ly ddaitfafrceonutldrnoomtbcoenetvraolluagrtoeudpasttatips<ti0ca.l0ly5w(iDtuhnnDeutntn'esttte'ss). est. `Treatment-related effects on body weight gain were noted at lesser concentrations than that observed for mortality. By Day (the end of the exposure phase) treatment related effects on the change in body weight from Day 0 were noted in treatment groups equal to or greater than the 70.3 ppm dose. This trend continued into the observation period in that by Day 8, there was a statistically significant difference in the rate of body weight gain (p < 0.05) in mallards from the 70.3 12%), 141 (17%), 281 (60%) and 562 (87%) ppm treatment groups when compared to control mallards. However, while body `weight remained significantly reduced at treatment concentrations greater than or equal to 70.3 ppm, recovery in terms of weight gain was apparent in mallards that survived the exposure period. By study termination (Day 22), there were no significant or treatmentrelated effects noted on the total change of body weight (Day 8-15, Day 15-22 or Day 22) for any of the treatment groups. `To examine the impact of PFOS on growthof juvenile mallards, the growth rates for each treatment group were calculated for various time spans within the study (0-8 days, 8-22 days, and 0-22 days) (Table 4). (Data from the 562 and 1125 ppm treatments were not included due to greater than 25% cumulative mortality prior to day 8). Growth rate (day) was estimated with a general growth model (Equation 2) that was linearized and then fitted to the body weight data collected during the study. Wo = Woexp (Equation 2) Where: Wi is the body weight (g) ofa mallard at time (1)ofthe study `Wo is the body weight () ofa mallard at the onsetofthe study (t=0) 8 the overall growth rate (1/day) tit time (days) `Theregression resultsofthe growth rate data are in agreement with the body weight data. There was a marked reduction in the growth rate for the 281 ppm treatment as compared to the control group Day 0-8. The overall growth rate for mallards in the 17.6, 5 000531 3co5n.t1r,ol70g.r3o,upa.nd 141 ppm treatments did not differ from the growth rate of mallards in the "Table 4. Growth rates (1/day) for juvenile mallards exposed to PFOS via the diet for 5 ddayasayndosbseprveod fosrItT expTroeatsmenut greoupe(p_pm _PFO_S)_______ Days 0 87 16 31 03 a 281 D8082 0010295 001036 00002505 0000158 0010150 0010025 00006853 0G0r2o2w ais c0s0t8ma0teT o0y0n8e5r egrs0o0n7:6 _(L7 Bo0d0y7w7eigh (70g0ro%wth ie (007i23 mre)0m0,83 cFoenesdumCpotnisonumdputriiongn:theWehxepnosucroemppearrieodd infothecotnrteraoltsm,entthgerroeupwsaesquaal rteodauncdtigorneatienr tfhoacnd tthheese28c1onpcepnmtrtarteiaotnmsencton(tTianbuleed 5t)o.beFuorbtsheerrvmeodret,hrtohueghreDduacytio15n. inHofeweedvecro,nsbuympDtaiyon22at, nfoetedpecromnasnuemnpttiaonndrtahtaetstrheetmuamleldartdoscionnttrhoelrelmevaeilnsinigndtirceaattinmgentthagtrotuhpescfhfaedctreocfovPeFrOedS. was. Table 5. Average feed consumption (FC) of juvenile mallards exposed to various dieTtraeraytcmoennctentrations of PFFCOS * TC FC Coonptmr)ol 05days 0335 8 days 0329 8:22 days 0203 18776 00331216 0352 0349 0278 0275 357013 0032092 00323827 00224318 21411 00423621 0481 0279 0260 0211 1S16225 00226054 00134114 00226610 GsurrovsisvinNgecmraollpasryd:s Aolflhamlafltlhaerdpsentshaatt ddiaeyd 8d,uarnidngalthreemcaoiunrisneg omfaltlhaerdsstuadty,Deayll22ofwethree sfuibnjdeincgtsedin0claudgerdostshinneccornodpistyi.on,Folrossmaolflamrudssclfeoumnadssd,eaadltedruerdinsgplteheensctouldoyr,, tehmepntcyercorpospys atrnedatmeemnpttyrelgaatsedt.roiNnetecsrtoipnasly tracts results (Table 6). These finding were for most mallards that survived to considered to Day 8 and Day be 22. were unremarkable as compared to control resus. 6 GROG `diTcatbalrey6P. FNOuSmbexeprosoufregrdousrsinpgatthhoelosgtiucdaylpearnioomda.lies in mallards that died in response to 2`8N1ominal Treatmen6t2Dose (ppm)155 Finding Crop, empty. oy o fy 7 oes) 7 Emaciated 1 1 4 G.I. tract empty 1 1 1 GGiizzzzaarrdd,ceomntpetnyts bile stained 00 20 41 IKneteels,tipnraolmcionnetnetnts black and tarlke 00 00 21 KLiodsnseoyfs,mupsalcele mass 0 0 1 2 2 s SSppilceeenn,, sgmraalyl and pale 01 01 21 Spleen, pale 0 2 3 ThNoitnRemarkable 1 1 4 CfoorncmeanltlraardtsiotnhsatofdiPdFOdSuriinngTitshseuesst:udLyivaenrdcofnorcetnhtorsaetisoancrdiaftiaceadreatprsetsuednytetdersmeipnaartaitone.ly Scoelrluecmteddatfarowmermaellaalrsdos ctohlaltecdtieedd dfurroimngmathlelafridrsst astevsetnuddyaytseromfintahteiosnt.udyLwievreer asnaamlpylzeesd ftohre PsRtuOdSy,comnatlelnatr,danmodrttahleitdyatawaasreapsrseosceinatteedd iwnitThablPeFO7.S Dcuornicnengtrtahteifoinrsst sinevleinvedrsaytshaotf e7xicneethdeed1112155 ug/g. ppm, 5T6h2ispcpomncaenndtra2t8i1onppwamstartetaatimneendtsi,n the liver by respectively. Day 3, Since Day 5 and mallards Day. were cnootndsuacmtpeldetdoienvaalsuaytsetetmhaetiucptmaaknenaenrd, aelpihmianramtaicoonkiknineettiiccsaonfalPysFiOsSofinthtehedsaetamaclolaurldds,not be Table 7. Average concentrations of PFOS (ug/g) in the liver of select mallards with treatment related mortality during the acute dietary study. Day 3 Nominal P6F2OS Dose (ppm) 1725 7 5 NNoomomrotratlailtiyy 2N1o6mo(rNta=l1i)ty T1I60O ((NN-=2)) 67 N1o48mo(rNta-l1ity 118907 ((NN==11) 112768 ((NN=-21) lllyAmlsaleTlvdaerrdnsoPtfRrhOoeSmmcaaondrcoeesnsetortafhtaitiodndisedrtehopaontrwtteehrdessuipnbeujcnieifctisetdeofddayyt.ga'nNaloWyrseibtse.Wreiighpt arTehentGstirheepennutmbaenrosfvesaagmeploers 7 CRORES tShaamtpPleFsOSofwlaivserreatnadinsederiunmtchoelelxcptoedsefdrommalsluarrvdisvifnogllmoawlilnagrdtsheonfivDeaydsayeaxnpdos2u2reinpdeirciaotde (Table 8). Table 8. Measured concentrations of PFOS in the liver and serum collected from `malTlraerdastomnenDtays 8 and 2T2ovferthe Dsatyudy"Serum Tver Day Serum i ml `Co1n87t7r6ol 64<66L5724O21Q9500 7338<81L5O61Q8966 || 2100<34L40O04Q322 4143<9L25O00Q750 73013 21S734134988 4380152987159 || 93231645112375 6181234:179499 124811 53586844416019 6S53892%612898 || 91220744342816 113849475.5914 SaRmTpcloens0cweenrreaeosns hTaengh5oe9ms5imt4eao2fru3sa5stnioFanm.1e0dL7OGG+e-4vi60.t50o0n8|ogrmwe2t0rw2ue4img,7hLt8Ob1Gs- 0033g4l04v1e5)4 tTDhhaeeybal2v2o.eordaTgshecerrruaemtiwoteoorfethcanotoncisneintgthnrieaftilicivaoennrts(doSiffLfP)eFrfOeonSrceafslolrimnaaltllhledaorSs.dLes wgrraaostuipo1s.b7e(3ctowonenterDnolamynaol&teaianncndldu1df.ee6d4m)aolinen bmaeltlwaeredsn,trthaetrmeefnortedtohseeseanrdatsieosruwmearnedmloitverrecpoonrtceedntrsaetpairoantseloyf. PFTOhSe woevreeranltrelilnaetaironosvhiepr btheetweenetnirePFdOoSsetrreaantgmee,ntradtohesre athned rleivleartiaonnsdhispesruwmerPeFOloSgacrointchmeinct.ra|tiTohnse mreealsatuiroendshoipns Da8ayre given Liver PFOS (ug/g=) 14.49*Ln(Dose, mg/kg-) 32.51 R=0.7967 Serum PFOS (ug/ml) = 21.39*Ln(Dose, mg/kg) --45.49 Ri=0.7461 coAonsncDewaniyttrh2a2ttihwoeensrSeoLnnoDtraaatyniao8sl.,yzTtehhdeerserienlcwaeetrgieornoswnhtoihp sbaeenxtdwdsepeeepncuirPfaiFtciOoSdnidfmfoaesryeenahcnaedvsetiisnsisgunseiefcrioucnamcnetalnyntrdaalttlieiorvenedsr sttheatrieolnast.onships, and th information gained would not be applicable fo environmental TPhoists edxepcorseuarsee, PwFasOSmocosntcelnitrkaltyiodnuseintolivtewrodecfarcetaosrse,d gthrroowutghhoduitlutthieonreaconvdereyxcpreertiioodn.. lAesvselusm,i2ngsitmheplbeioolnoeg-iccaolmpmaercthmaennitsmmofdoreleliwmaisnactimopnlowyaesdtthoe cssaimmeatate ltohweerlobssodoyfbPuFrdOeSn cfvraolmualeivserthedureatetoofdleopsusroatfiPonFOfSrofmromthelivmera:llards(Equation 3). The following model 8 Ross C=Cyrep (Equaion 3) Where: CCoyjiis tthheeccoonncceennttrraattiioonnaatthteiomnes(1e)tduorfienlgimeilniamtiinoanti(ogn/pgh)ase. ktiisstitmheeo(vdearyasl)l elimination rate constant (day) "cTohneceonvterraatliloneliinminliavteironplroattteedcoansstaanftun(ckt")iownaosfetsitmiemadtuerdinbgy tdheeteerlmiimniniantgiotnhepshlaospee oofftthhee sattuedyco(nsDtaaynst8t-a2k2)e.s inHtoowaecvceoru,ntiloisssiemspofrrtoamnttwtoo rcoenmceumrbreenrttphraotcetshseeess,tilomsasteddueeltiomignraotwitohn dtoilutthieonealinmdineaxtcireotnioonf. PIFnOaSddiftiroonm, tmhaelsleartdwso bparsoecedssoens magaey, dsiefxferanind ismepaosront.anceForerlatthiivse asnuaclhystihsa, itthweaosvearaslsluemleidmitnhaattiobnotohrplrooscsecsosuelsdabcteerdepinreasnenitneddeapsenfdoelnltowsb:ut additive manner Keka+g rInectohridsecdqudautriionn,g thies stthuedyg,rowwhtihleratke'thwaatswaessteismtaitmeadteads ftrhoemovbeordayllweriagtehtomfedaescurreeamseentins mweaassuersetidmaltiveedr (PkFs)O.S cBoansceendtroantiotnhse.meOanscuerekd' acnondcgenwtrearteiocnaslcuolfatPeFd,OStheineltihmeinaltivieorn arnatde gartoewctohnsrtaatnetsseswteirmeateesdtifmraotmedbfoodrythweei8g.h7t,s measured 17.6, 35.1, between Days 70.3, 141 and to 281 p22p,mcltirmeiantamteinotns c(oTnacbelnetr9a).tioBnass)edwoans th0e.s0e39r7esuldtasy,'thewiatvheraageheallifmliinfaetioinn rtahtee ofliPveFrOSest(ibmaasteeddontolivbeer approximately 17.5 days. Texacbrleteio9.n Grartoewctohnsrtaatnes, floirvePrFaOnSd isnerjuumveneilliemmianlaltairodns,constants, and overal liver Treatment Whole Body Growth Rate Liver To Seam ke (mg PFOS/kg) (day Control 0.0586 (day) NA (dNaAy! (dNayA 18776 00.00564376 0001801717 000026a4 o00d7i7n6 3750.31 0000556950 O00I76L7 0000517476 00.11101675 218411 0000582238 0010488211 0000269583 0o.i0s76i5 waArtse cton)sasbaed on fata dered From essere Rds Garin Tecovry perod NA non spplcable 9 e0ans oCobnsceernvterdatfioornlsivoefr PPFFOOSS cionnctehnetrabtliooonds.seFrourm tdheecrpoesatseedxpionsuaresipmeirliaord,mDaanyner tahsrotuhgaht wDiatyh a22n,etshteimaavteerdaghealefliimfinoaft6io.n86ratdeaycso.nstWahnetn(kcoo)mfpoarrPeFdOtSo lfirvoermcsoenrcuenmtwraatsio0n.s10o1vedrathye osfamPeFeOxSperinimtehnetaslerpuermiowda,ssearpupmrocxoinmcaetnetlryatfioonlsddelcersesatsheadnatthaegersetaitmeartaetde.haTlfhelifhealifnltihfee liver. These results indicate that while PFOS is rapidly climinated from serum, the losses bfordomy bliuvredreanndafotetrheerxptoisssuureessarheavselocweearsead.ndIanreadmdoitrieonl,ikiteliystaoscsounmteridbutthea10thae sbiigonliofgiiccaanlt o`rmemcohraeniosfm elimination tfhoer can eiblneiitmeaixlntarbtaoipdoonylaitbseudtrhdteoesnlaormweeemraatibnoledodyweabrtubrddoaedyny2l2be,uverlidtse,ins lienvdelest.ermSiinnactee tfypitcheallryae20o%f Conclusions: `LTDheS0diebtaasrcyd 8o-ndaAyDLICwSa0sf1or50PmFOgSPFwOaSs/6k0g3bpodpymw(eCiIgh=t4d3a1y-(9C3I8=1p1p7m-)20w1himlge tPhFeO8S-kdga.y PboFdOyS/wkegigbhotddyayw)e.ighTthdeayn)obamsoertdalointy$ dcaonycsoenftreaxtpioosnurew.asBa14s1edpopnmthPeFrOedSuc(toiron6o1f.3bomdgy dweeitgehrtminaetdDtaoybe8,35t.h1e pNpom O(borser12v.a0blmegAPdFveOrSs/ekgEfbfoedcty Cwoenicgehnttirdaatyi)onwhi(lNeOtAhEeCL)owweasst bObosdeyrvwaebiglhetiAddavye)r.se EffTehcet CdonacyentNroatiOobnse(rLvOabAlEeC)Adwvaesrs7e0.E3ffpepctm L(eorve2l0.(4NmOgAEPLF)OSaknde wLeorweest15.O4bsaenrdva2b0l.e7Agdv/egrsPeFEOfSf,ectreLspeevcetliv(elLyO.AETLh)e v$adluaeys NbaOsAedEoLn alnivderLcOoAncEenLtrvaatliuoenss tbhaeseNdOonAEseLruamndconLcOenAtEraLtiovnasluweesreba3s0e.d5 aonndc4i8t.h1erugli/vmelr PanFdOSs,erreusmpecctoinvcenliyr.atHioownesvearre, cDoanyse8r,vatthirveee edsatyismaptoesst-oeftxhproessuhroeladndvalduoeess. nTothitsakies bienctoauascecotuhnetsepodtaetnatiwaelreclciomlilneacttieodnoonf `PgFeoOmSetrbiectwmeeeannodfayNsOSAEanLd 8. and LTOheAENLO)AaEreL,suLmmOaArEiLz,eda(nTdabtlhere1s0h).old doses (TD) (the Table 10. NOAEL, LOAEL and threshold doses (TD) of PFOS derived fnrodm aaT mallard a-- cute feedo ing studR y. * T--T-- ADiveetraargye(Dpapiml)y Dose (mgkgldsy) 3152.10 270043 419576 LSievreurm ((pugg/g/)ml) ATvaloesbased on he educios of 310554 odywerght mn resed 2480.71 mands 231843 Lisvnd escrr FOS concenrations messed from Day samples NSOeArEuLm aannddleLrOAfEeLctvavluueessfoarsAmDIesansdedidetalaroesbased on dietary concentrations. DATA QUALITY: Reliability: Klimish Ranking = | 10 600s 3a References: tDruenantemtetn,tsCwiWt.h a 1c9o5n5t.rol.AJ. Ammuelri.plSetatciso.mpAassroics.on5'0s:1p0r9o6c-e1d1u2r1e for comparing several LGaClSl0aghSetru,dSy.Pw.i,tChastchye, MCa.lSl.a,rdB.eavAemres,nd1.eBd., RaenpdorVta,nWHioldvleinf,e RI.nL.tem2a0t0i4,onaPlFOSL:tdA. DPireojteacrty NO. 454-102. Gulley, DID. 1990. TOXSTAT Release 3.2. The University of Wyoming. Hansen, K. 2001. (Sera and Liver). Analytical Revision 1.Re3poMrtEonfvDiartonamefnotraPlFLOaSboDriaettoarryy. LRCeSpo0rtStNuod.y: wFitAhCTMa-lTlOarXd.s 130, SAS Institute. NC.USA. 1999. SAS/STAT User's Guide, Release 8.02 Edition, SAS Institute, Cary PWuabglniecra,tioJnGs.Inc.1,97H9a.milFtuonnd,amIelnt4a6l1sp.of Clinical Pharmacokinetics. Drug Intelligence OTHER: Last Changed: 5/05/04 n CEOCBG