Document M4QmXQOV5Gj8xkg5Zm15BOQ0k

( ( ! FOR DUPOHT USE ONLY AR226-2926 HASKELL LABORATORY REPORT NO. 180-85 Approximate L ethal C o n c e n tra tio n by I n h a la tio n (ALC? o f E. I . du Pont de Nemours and C o ., In c . Haskell Laboratory fo r Toxicology and In d u stria l M edicine Elkton Road, P. 0 . Box 50 Newark, Delaware 19714 Company Sanitized. Does no! contain TSCACB1 Date Issu e d : A pril 18, 1985 ADorox1nate J L e t ^ ^ ^ ^ | ^ ^ | | ^ ^ 3 ^ t1on *ALC) of Summary occurre th is te st, ,)BR r a t s were exposed to d u s t atm ospheres o f :o r a s in g le , 4*hour p e rio d . No d e a th s exposure w up to 2100 mg/nT. Under th e c o n d itio n s o f co n sid e red t o have very low a c u te t o x i c i t y by in h a l a ti o n . Work by: ^ -- . A -fU T lA . ThomasA. Kegelman V /jg /s5 Supervised by: Laura A. Kiinnnnevy * Chemist Approved by: n-- H U J l . Nancy *C . Chromey, v h .D . Study D irector Section Supervisor Acute In v estig atio n s Acknowledgements: J ru c e A. Burgess a ls o p a r t i c i p a te d in t h i s stu d y . LAK:HLR 9.3 t contain TSCACB1 Uized.Doesno - 2- I Haskell Laboratory Report No. 180-85 Sponsor: Polymer Products Department E. I . du Pont de Nemours and C o ., In c . Wilmington, Delaware 'Polymer Products Department E. I . du Pont de Nemours and C o ., Inc. Wilmington, Delaware Test F acility : Haskell Laboratory fo r Toxicology and ------------------- I n d u s tr ia l M edicine E. I . du Pont de Nemours and C o ., In c . Elkton Road, P. 0 . Box 50 Newark, Delaware 19714 Study Initiated/C om pleted: 6/21/84 - 7/6/84 There are 6 pages in th is rep o rt. TSCA CBl S a,,W a . o n o . c o ''.a<n - 3- INTRODUCTION vas to determ ine a 4-hour I n h a la tio n ALC f o r _______ _______________ fin male r a t s . The ALC was d e fin e d as th e low est atm ospheric concentration te s te d th a t caused the death o f 1 o r more r a t s e i t h e r on th e day o f exposure o r w ith in 14 days po st ex p o su re . MATERIALS AND METHODS A. Animals Young a d u lt male Crl :CD*(SD)BR r a t s were receiv ed from C harles R iv er Breeding L a b o ra to rie s , Kingston. New York. Each r a t was assig n e d a unique 6 - d ig it id e n tif ic a tio n number which was recorded on a card a f f ix e d to th e c a g e. R ats were quarantined f o r one week p r io r to t e s t i n g , and were weighed and observed tw ice during th e quaran tin e p e rio d . During th e t e s t , r a t s were in housed p a irs in 8" x 14" x 8" suspended, steel-m esh cages in rooms m aintained a t 25-51% r e l a t i v e hum idity and 20-26C on a t im e r - c o n t r o ll e d '12 hour/12 hour lig h t/d a r k c y c le . The r a t a ssig n e d th e lower number in each cage was id e n tif ie d by a s la s h in th e r ig h t e a r . R a ts' t a i l s and cage cards were color-coded w ith w a te r-in so lu b le m arkers so th a t individual r a ts could be id e n tifie d a f t e r exposure. Except d u rin g exposure, Purina C e rtifie d Rodent Chow9 #5002 and w ater were a v a ila b le ad lib itu m . . B. Exposure Protocol Groups o f 6 r a t s , 8 weeks old and w eighing between 218 and 261 grams, were restra in e d in p erfo rated , s ta in le s s s te e l cy lin d ers w ith conical nose p ie c e s . Each group was exoQsed nose-o n ly f o r a s i n g l e , 4-hour period to a dust atmosphere o fQ H P > n a i r . Rats were weighed p rio r to exposure, and were observed fo r c lin ic a l sig n s during exposure. S u rv iv in g r a t s were weighed and observed d a lly f o r 14 days p o st e x p o su re, weekends and h o lid ay s excluded except when deemed n ecessary by th e r a t s ' condition. C. Test M aterial S tability: The t e s t m a te ria l was assumed to be s ta b l e th roughout th e exposure phase o f th e s tu d y . The amount o f c o n ta c t o f th e t e s t m a te ria l w ith room a i r was minimized throughout th e t e s t . D. Atmosphe c G eneration' Oust atmospheres o f i f l H l n a ir were generated with a 2-stage* v e r t i c a l g la s s generatO F7 A" round f la s k served as a d u st r e s e r v io r . A ey elone-shaped fla sk * in s e r te d above th e r e s e r v io r , served as an e lu tr ia to r . A motorized s tir rin g rod with p la s tic paddles a g ita te d dust in th e g en erato r. A ir introduced a t the re s e rv o ir blew dust p a rtic le s upward to th e e lu t r ia to r . A ir introduced a t th e e l u t r i a t o r swept d u st p a r t i c l e s in to th e exposure chamber. The atm ospheric c o n c e n tra tio n was c o n tr o lle d by varying jthe 2 a ir f lo w s . A nalytical The atm ospheric c o n c e n tra tio n o f ^ l H l w a s determ ined a t ap proxim ately 30-m inute in te r v a ls by drawing c a lib ra te d volumes of chamber atm osphere th ro u g h preweighed g l a b f i b e r f i l t e r s . F i l t e r s were weighed on a Cahn model 26 Autom atic E le c tro b a la n c e . The atm ospheric c o n c e n tra tio n o f p a r t i c u l a t e was determ ined from th e f i l t e r w eight d i f f e r e n t i a l b e fo re and a fte r sampling. ,. P a r tic le s iz e d is tr ib u tio n s (mass median diam eter and p ercen t re s p ira b le ) were determined w ith a S ie rra Cascade im pactor during each e x p o su re *1. Chamber tem p eratu re was m onitored w ith a m ercury therm om eter, r e l a t i v e hum idity was measured w ith a Bendix model 566 p sychrom eter, and chamber oxygen c o n te n t was m onitored w ith a BioMarine model 225 oxygen a n a ly z e r. F. Records R etention A ll raw d ata and th e fin a l rep o rt w ill be sto re d in th e arch iv es o f H askell Laboratory fo r Toxicology and In d u s tria l M edicine, Newark, D elaw are, o r 1n th e DuPont Hall o f R ecords, E. I . du Pont de Nemours and C o., W ilm ingto', Delaware. 1 C a lc u la tio n d e sc rib e d 1n S ie r r a In stru m e n ts, I n c ., B u lle tin 7-79-219IM, In s tru c tio n Manual: S eries 210 Ambient Cascade Impactors and Cyclone P re se p a ra to rs. ~ - 5i iipany Sanitized. Does not contain TSCA CB RESULTS A. Exposure C onditions ahd A ssociated M o rta lity Dust atm ospheres Were v i s i b l e In th e chamber d u rin g each e x p o s u re . Chamber tem p eratu re rjanged between 26-28C, r e l a t i v e hum idity ranged from 57-60*, and chamber oxygen c o n te n t was m aintained a t 21*. A tm ospheric c h a ra c te riz a tio n and associated m o rta lity data are presented below. Atmospheric C h a ra c te riz a tio n o f TBSA and Associated Rat M ortality O; C o n c e n tra tio n (mg/m ) Mean S .P . Range 820 160 610-110 R e sp*ira b le a 74 MMDb 5 .5 urn M ortality (# d eath s/# exposed) 0/6 2100 1100 1000-4000 53 9 .5 urn 0/6 a P ercent by weight of p a r tic le s w ith aerodynamic d iam eter le s s th an b 10 urn. D Mass median aerodynamic d ia m e te r. No a d d itio n a l exposures to h ig h e r atm ospheric c o n c e n tra tio n s were t e s t e d due to a lim ited quantity of te s t m aterial av ailab le fo r te s tin g . B. C lin ic a l O bservations During or immediately following exposure, ra ts ex h ib ited nasal and ocu lar d isch arg es, labored b reath in g , gasping, leth a rg y , and had t e s t m a te ria l on t h e i r facb s and head s. R ats were s t i l l l e th a r g ic 1 hour p o s t ex p o su re . Rats exposed t o 820 mg/nr had a dim inished s t a r t l e response when th e chamber was tap p ed . During the postexposure p erio d , ra ts lo s t approxim ately 1-8* of i n i t i a l body e ig h t 1 day a f t e r ex p o su re, follow ed by normal w eight g a in . No s ig n i f ic a n t adverse c li n ic a l 's ig n s were observed d u rin g th e 14-day postexposure period. CONCLUSION UUnder th e c o n d itio n s o f th is t e s t , th e In h a la tio n ALC fo r was g r e a te r than 2100 mg/m . This m ate ria l i s co n sid e red to have( v e r y "low a c u te t o x ic it y by in h a la tio n (ALC g re a te r than 2000 mg/m ) . - 6Company Sanitized. Does not contain TSCA CBI