Document LprRmjzRN257gLqrJ2GJ1bJkq

AR226-2832 HASKELLLABOR A TO R YFQ R TOXICOLOGY ANpINDUSTRIALMEDICINE Company Sanitized. Deee notcontain ISCACBI NINETY-DAY FEEDIN^ S T U D Y IN RATS AND DOGS W I T H | H | | ^ H Haskell Laboratory Report No. 68-73 I. Does not contain TSCA G B i NINETY-DAY FEEDING STUDY IN RATS AND DOGS WITh B H B H I Haskell Laboratory Report No. 68-73 Approved for Biochemistry: ,VvL4'' l^ohn R. Barnes Chief, Biochemistry Section Approved for Pathology: n/MZ / H U J /A7 . -./-/ U v , / / James G.- Aftohmis Chief, Pathology Section Approved by: //Y~ e t,? 7 *" Charles F. Reinhardt Assistant Director H S :dhg Date: February 23, 1973 Sanitized. Does not contain TSC/ Company NINETY-DAY FEEDING S T U D I E N RATS AND DOGS WITH H H H H Haskell Laboratory Report No. 68-73 SUMMARY been fed to rats and dogs for ninety days at dietary levels of 0, 500, 750, and 2,500 ppm. No nutritional or clinical signs of toxicity were observed in rats or dogs on any of the test levels. receiving the intermediate and highest dietary levels of H p i H I U H s h o w e d lower erythrocyte counts, hemoglobin concentrations and hematocrits than those of the controls; those receiving the highest dietary level also showed a slightly higher incidence of hematuria and____ proteinuria. Male rats receiving the two higher dietary levels of^^JHjj^H showed slightly larger liver and kidney weights and higher o r g a n / b ^ ^ ^ ^ ^ ^ ratios than did the controls or other test group; all female test rats had higher kidney weights and kidney/body weight ratios whereas the female rats on the highest dietary level also had greater liver weights and liver/body weight ratios. Of all the organs examined histologicallv^on^^he livers, and only those from the male animals receiving 2,500 p p m f l m ^ H | B | showed changes, and these were considered to be reversible. * Dogs receiving the'highest dietary level of [had lower erythrocyte counts, hemoglobin concentrations and hematocrit values than did the controls. Alkaline phosphatase activities were elevated in the dogs in all test groups, whereas cholesterol values were higher in dogs receiving 750 and 2,500 ppmfl^fljj^^Uft The livers of the dogs from all three test groups were neav^^than those from the control dogs. The lrver was the only organ of those examined that exhibited histologic changes and this was confined to those coming from the highest level group. , Thus, the results of this ninety-day feeding study and those of the previous one support only 100 ppm as a "no-effect" level for i Company Sanitized. Does not contain TSCA Ci C NINETY-DAY FEEDING,STUDY IN EATS AND DOGS WITH i A " * Haskell Laboratory Report No. 68-73 b This feeding study was performed by Mrs. Kathleen Carroll under the direction of Dr. Henry Sherman. The clinical laboratory and biochemistry tests were performed by Mrs. Louise L. Adams, Mr. Norman W. Henry, III, Mr. John R. Pennington, Jr., and Miss Adele M. Pochomis under the direction of Dr. John R. Barnes. Gross pathology and preparation of slides were carried out by Dr. Rudolf .i Culik, Dr. K. P. Lee, Mr. August H. Stenholm, Mr. William I. Swan, Mr. Francis Ulmer, Mr. Anthony T. DiLorenzo, Mrs. Jean A. Houck and Mrs. Joan A. Dimeler under the direction of Dr. James G. Aftosmis. Histopathologic evaluation of the tissues was conducted by Dr. Edwin F. Stula under Dr. Aftosmis' direction. i ' !!zed. Does not contain T S C A C NINETY-DAY FEEDING STUDY IN. RATS AND DOGS WITH ] Haskell Laboratory Report No. 68-73 INTRODUCTION |i|^^ E | ^ E B T h a s low acute oral toxicity for the male rat, its ^5p?oxim^e Lethal Dose (ALD) being greater than 11,000 mg/kg of body weight. The compound produced an increase in the liver weight of the animal dosed at 11,000 mg/kg and possibly in the animal dosed at 7,500 mg/kg. The compound shows high cumulative oral toxicity. When administered to each of six rats at a dose of 5,000 mg/kg/day, all died after having received three to five doses. When administered to rats at a dose of 2,250 mg/kg/dayj it produced heavy livers in animals sacrificed after the tenth dose with evidence of some recovery when the animals were sacrificed after a 14-day recovery period. The histologic changes observed in these large l ^ e r ^ v ^ ^ y ^ ^ t f ^ ^ e ^ ^ u s u a ^ ^ a s s o c ^ ^ e d with degenerative changes Repeated doses of 220 or 22 mg/kg/dayd^lnot ^ p r oduce anv evidence of liver enlargement or pathology A ninety-day feeding study in rats and dogs has already been c o n d u c t e d H H H j j f t Both species were fed, for 35 days, at dietary levels'of ^ ^ ^ ^ 0 ^ 5 0 0 , and 2,500 ppm; after this period of time, the 500 ppm and 2,500 ppm dietary levels were doubled to 1,000 ppm and 5,000 ppm for the duration of the study. Compound-related changes were observed primarily in the liver of both species of animals at the intermediate and highest level of feeding, i.e., 500-1,000 ppm and 2,500-5,000 ppm; these included gross changes and increased liver weights. Histologic evidence of hepatic changes was observed only at the highest dietary level, i.e., 2,500-5,000 ppm. Thus, 100 ppm was considered the "no-effect" level. In -order to help obtain broader FDA sanction to ' u s e ^ p p ^ m ( g o n paper products in contact with food, the present ninety-day feeding study in rats and dogs was undertaken in an attempt to determine whether a higher "no-effect" level than that obtained earlier existed. TEST MATERIAL The test material was supplied by the Organic Chemicals Company Sanitized. Does net contain TSCA CB! 2 A. RATS PROCEDURE i Fifty male and 50 female weanling albino rats (ChR-CD) were housed in pairs, sexes separate, in suspended stainless*steel wire cages and fed ground Purina Laboratory Chow (GPLC) with 1% corn oil (CO) added. During a pre-test period of five days, the animals were observed with respect to food consumption, eating habits, and weight gain. They were divided into four equal average-weight groups of 10 male and 10 female rats each at the end of this period on the basis of weight gain and freedom from gross respiratory disorders or other clinical signs of disease. The groups were then assigned at random v to receive the following diets: Group Computer Group s- Diet I (Control) II III IV (1 and 2) (3 and 4) (5 and 6) (7 and 8) GPLC + GPLC + GPLC + GPLC + 1%CO 17oCO + 500 ppm 17oCO + 750 ppm 1%CO + 2,500 ppm ^ The highest dietary level (2,500 ppm) had been suggested by the FDA to act as a bridge between the present stijdy and the one con ducted earlier under ingredient, An amount of w ^ e ^ ^ re^^u^ ^ ^ L ^ t h ^ ^ ^ ^r^^^^let equivalent to that con tributed b y t t H | | | M | | H H M | M t o the diet in Group IV. Diets were prepared f r e s l ^ a ^ ^ e e l ^ n ^ s t o r e d at refrigerator temperature until used. The animals were weighed once a week during the entire study. Food consumption data were obtained on a group and sex basis at the times the animals were weighed. During the test, the animals were examined routinely for any abnormal behavior and any clinical manifestations of toxicity. Hematological, urine and biochemical analyses were conducted on ten male and ten female rats from each group after they had been on their respective diets for one, two, and three months._ Hematological evaluations included an erythrocyte* count, a measure hemogidbin con centration, a measure of the hematocrit, and a total and differential white blood cell count. Urine analysis consisted of a measure of the 24-hour urine volume, concentration in milliosmoles and creatinine, a test for sugar, blood, protein, and urobilinogen, and an observation of the color, appearance, and pH. Specimens with a negative test for blood were combined to form two pools of urine for each group and the sediment rom these pools examined microscopically. All specimens with a positive test for blood were examined separately. In the biochemical tests, to measure liver function, alkaline phosphatase and glu^amic- pyruvic transaminase activities uitiLU^iu Cuuc.cuLialIuu measured in blood taken from the tails of 10 males and 10 females in each group. wOitittRv Sanitized. Does not contain TSC CBt 3 A. RATS (Continued) PROCEDURE (Continued) After 91-98 days of continuous feeding, all the animals in each group were sacrificed by CHCl^ administration. The following organs were weighed: brain, heart, lungs, liver, spleen, kidney, testis, stomach, adrenal, -and pituitary. Organs or tissues, preserved in formalin and stained with hematoxylin-eosin, included, in addition to those listed above, the following: eye, exorbital lacrimal gland, sciatic nerve, skin, mammary gland, bone marrow, lymph node, skeletal muscle, trachea, aorta, salivary gland, esophagus, colon, cecum, duo denum, urinary bladder, prostate and seminal vesicles, uterus, Fallopian tubes, ovary, thyroid, parathyroid, and thymus. The above tissues from the control and highest level group (2,500 ppm) were evaluated histopathologically; only the livers from the animals in the other two test groups were examined histopathologically. RESULTS 1. Weight Gain " Average body weight curves for control and test groups of animals are plotted in Figure 1; average body weights and average weight, gains are summarized in Tables I,;. II, V and VI. * The presence of 2,500 p p m ^ ^ ^ J J H | i n the diet of male and female rats did not adversely affect their rate of weight gain. 2. Food Consumption ' A summary of the average daily food consumption data, computed as grams ingested per rat for each group, is presented in Tables III, IV and V. There were no meaningful differences among the control and test groups with respect to the amount of food they consumed over the entire test. 3. Food Efficiently Food efficiency data, calculated as gram weight gain per gram of food consumed, are presented in Tables III-V. There were no meaningful differences among control and test groups with respect to food efficiency. f I Company Sanitized. Does not contain T S C A C B i A. RATS (Continued) RESULTS (Continued) 4. Dose The average daily ingestion of during each week was calculated in milligrams o f ^ P ^ m p p e r kilogram of body weight; these data are presented in Table VI. The decline in the average dose of test material received by animals observed in this study is normal and typical of that observed in most feeding studies where rapidly growing animals are used initially and the concen tration of test material is kept constant throughout the test. 5. Clinical Observations None of the test animals in any of the three test groups exhibited any clinical signs of toxicity during the entire test that could be attributed to the test material. 6. Mortality None of the animals in the control and test groups died during the entire feeding study. I 7. Hematology The results of the periodic hematologicaLexamm^ tions conducted on rats fed the various levels o f f and their controls are summarized in Table VII. The erythrocyte c o u n t c o n c e n t r a t i o n and hematocrit of the rats fed J ^ H P j ^ p w e r e lower than those of the controls at 750 ppm and above. A one-way analvsisnf variance showed that the treatment with ^ f l B H B P h a d a significant effect on these measurements. Th^vaiuies for these m e a s u r e m e n t s ^ o ^ ^ ^ d in the males but not the females fed 750 PPm PHj|jjij|^ were signifi cantly (p < 0.05) lower than those for the controls. Both sexes were affected by a level of 2,500 ppm in the diet. The calculated hematologic indices, mean corpuscular volume (MCV) and mean corpuscular hemoglobin that the erythrocytes of the rats fed 2,500 p p m j ^ ^ ^ H ^ S 5 were macrocytic and hypochromic. There were no effects on che number or distribution of leucocytes. Com pany Sanitized. Does not contain T SC A CBS 5 A. RATS (Continued) RESULTS (Continued) 8. Urinalysis The results of the periodic urine analyses conducted on rats fed various levels o f ^ H H ^ ^ ^ ^ a i r e summarized in Table VIII. A slightly higher incidence of hematuria and proteinuria occurred in the rats fed 2,500 ppm] than in the other groups. All other measurements and observations made on the urine of the treated rats did not differ from those of the controls. 9.- Biochemistry The results of the periodic biochemical measure ments conducted on the blood of rats fed various levels of [are summarized in Table IX. No effect on any of the biochemical measurements was found that could be related to the presence of the |Ln the diet. 10. Pathology A summary of the average weights of select organs taken from the control and test groups is presented in Table X; organ/body weight ratios are summarized in Table XI. Among the test groups, ma^^animals receiving theg intermediate and high levels o f ^ H j jj^^Pshowed higher average liver and kidney weight^^bothliver/body weight and kidney/body weight ratios were also slightly greater in these groups. Among the female test.,groups, jail showed higher average kidney weights and kidney/body weight ratios than did the controls; average liver weights and liver/body ^weight rat^^wer^slightly greater in the animals receiving 2,500 in the other test groups or the control. . -= A summary of the histopathologic findings is presented in Table XII, where 0 = no abnormalities detected, + = slight degree of lesion present, and X = organ not on slide. .i Test chemical-related histopathologic effects were found only in the livers of m a l ^ r ^ ^ ^ ^ e d the highest (2,500 ppm) dietary level o f S ^ E x a m i n a t i o n of the livers from rats that received the two lower levels of the test chemical did not reveal any compound-related effects. Com pany Sanitized. Does not contain T S C A CB* 6 A. RATS (Continued) RESULTS (Continued) 10. Pathology (Continued) The liver changes observed in the males in the highest dietary level group consisted of fatty change, cytomegaly, and cytoplasmic hyaline droplets. This type of liver change is considered to be reversible. B. DOGS PROCEDURE During a pre-test period of approximately one month, 16 male and 16 female beagle dogs, 9 to 14 months old, were given Wayne Dog Krumsi and water ad libitum between 3:00 p.m. and 7:00 a.m. Animals were examined daily for any abnormal behavior and any clinical manifesta tions. of toxicity. During this period, specimens of blood and urine were Collected from each dog twice for clinical laboratory examina tions. The tests included in these examinations are listed below: Hematology: Erythrocyte count, hemoglobin concentration, hematocrit, total and differential leucocyte count. Urinalysis: An observation of color, appearance and pH; a measure of the 24-hour urine volume, creatinine, and osmolality; a test for sugar, blood, protein, acetone, urobilinogen, and bilirubin; a microscopic examination of the sediment. Biochemistry: Glucose,urea-nitrqgen, creatinine, cholesterol, alkaline phosphatase, glutamic-pyruvic transaminase, bilirubin, total protein, albumin, and albumin/globulin ratio Four males and four females were allocated to each of the four groups on the basis of normal clinical and nutritional evaluations and assigned at random to receive the following diets: i' Group Diet I (Control) II III IV Krum^ + 750 ppm| Krums + 2,500 ppm Diets were prepared fresh each week. Diet was offered ad libitum to the dogs between 3:00 p.m. and 7:00 a.m.; -water was available at all times. Company Sanitized. Does not contain T S C CBI 7 B. DOGS (Continued) PROCEDURE (Continued) Diet consumption and body weight data were obtained each week and calculationswere made to determine the approximate daily dose each week o f ^ ^ ^ ^ ^ H u p e r kilogram of body weight. Animals were examined daily f o r a n ^ c ^ n i c a l signs of toxicity. The same clinical laboratory examinations made during the pre test period were conducted on each dog after one, two, and three months of feeding. After 98-105 days of continuous feeding, all dogs were sacri ficed by electrocution and submitted to gross and histopathologic evaluation. Organ weights were obtained for the following: brain, heart, lungs, liver, spleen, pancreas, kidney, testis, prostate, stomach, thymus, adrenals, pituitary, and thyroid. Organs and tissues were preserved in formalin and stained with hematoxylin- eosin; these included, in addition to those mentioned above, the following: epididymis, Fallopian tubes, aorta, esophagus, uterus, ovary, duodenum, cecum, mammary gland, urinary bladder, spinal cord, trachea, salivary gland, bone marrow, lymph node, colon, sciatic nerve, skeletal muscle, eye, and skin. All tissues taken from the dogs on the control diet and highest test level diet were evaluated histologically; only the livers from the animals receiving the low and intermediate levels o f | H H t t l H w e r e examined histologically. The livers from the contro^anc^^^rast level dogs were stained with Oil Red 0. ' RESULTS 1. Body Weight The weekly body weight data of the individual dogs are presented in Tables XIII and XIV. All dogs, control and test, showed normal body weight gains during the study. 2. Diet Consumption Values calculated for average daily diet consump tion for each dog are presented in Tables XV and XVI. The amount of diet consumed by each dog varied from week to week. However, there was no adverse Effect by the test material upon diet intake. 3. Dose Tins avsr*a0-s daily intalcs o i n mi 11i- grams per kilogram of body weight wa^ca^i^^ted for each dog; these results are presented in Tables XVII and XVIII. Company Sanitized, Does not contain TSC A CBS - 8- RESULTS (Continued) 3. Dose (Continued) Si^ice the body weights of most of the dogs : relatively constant throughout the test, the dose of eceived by each treated dog each week fluctuated diet intake. 4. Clinical Signs Regular examination disclosed no clinical changes in a n v o ^ t h ^ t e s t dogs that could be attributed to the feeding All dogs survived the 90-day feeding period without incident. 5. Hematology The results of the hematological measurements con ducted throughout the feeding study are summarized in Table XIX; the numbersrepresent average values obtained during pretreatment and treatment periods. Individual values- are presented in Appendix I. . The erythrocyte count, hemog^b^^^oncentration, and hematocrit of the dogs fed 2,500 P P n ^ m P j j H f ere generaH y lower than those of the other dogs, coirtro^ana test, during treatment. A one-wa^^analysis of variance indicated that the treatment witbMUBB||ftignificantly affected the hemoglobin concentration and hematocrit, whether expressed as grams of hemoglobin per 100 ml and percent of packed cells, or as the change from the pre-test observation for these measurements. 6. Urinalysis The results of the urine analysis measurements con ducted throughout the feeding study are summarized in Table XX; the numbers, represent average values obtained during the_pre treatment and treatment periods. Individual values are presented in Appendix I. No effect attributable to the addition of to the dogs1 diet was found in any of the measurements or observations made on the urine. 7. Biochemistry The results of the biochemical measurements conducted throughout the feeding study are summarized in Table XXI; the figures recorded represent average values obtained during the Company Sanitized. Does noi contain TSC A CBS 9 RESULTS (Continued) 7. Biochemistry (Continued) pretreatment and treatment periods. Individual values are presented in Appendix I. There was no effect on the glucose, urea-nit^pogen, 1 creatinine, transaminase, bilirubin, or plasma protein values. A one-way analysis of variance, however, showed that the total ch^estero^and alkaline phosphatase activity of the dogs f e d H ^ H H ^ n a r e r e significantly affected by the treat ment. T^^fflcauSe phosphatase activity of the dogs fed the lowest dose, 500 ppmfl^B^^HtfK was elevated significantly (p < 0.05). Both alS^^^^^^^phatase and c h o l e s t e ^ ^ ^ ^ ^ ^ ^ were elevated in the dogs fed 750 ppm and 2,500 This effect occurred after one month, was maximum at two. months, and then stabilized, or decreased slightly, at three months. The effect was significant for the relative change from the pre-exposure, expressed as a percent, as well as for the increase in Bessev units (alkaline phosphatase) or mg L (cholesterol) for the treated dogs. 8. Pathology The individual organ weigl^^^^Lthe dogs sacrificed after three months' feeding o f l | ^ ^ ^ ^ | ^ a r e presented in Tables XXII and XXIII. The small group sizes and ^.arge variation do not per mit a complete statistical analysis of the liver weights. However, a variance analysis of liver/body weight ratios indicates that; there wa| a difference between control and test groups with respect to this ratio, a heavier liver being the effect of treatment. This is summarized in Table XXIV, where mean values are recorded. It would appear that the livers of all threevroups^^L e., low, intermediate, and high d^etar^ IPlevels o f f i f H H H were affected by the presence oj .n the cfiet. A summary of the histopathologic findings is presented in Tables XXV-XXVIII. - The livers from the highest level of feeding were pale and had rounded edges with a tight capsule. Histo- , logically, the l i v e r f r o ^ t h e dogs in the highest; level of| feeding (2,500 p p m ^ B Q f l B f i was the only organ affected. The liver changes were difficult to detect. They consisted of a slight enlargement of hepatocytes (hypertrophy) together with an uneven distribution of cytoplasmic particles (degenera tion) . Examination of the livers from control and high level dogs stained with Oil Red 0 did not reveal an increase of lipid. Company Sanitized. Dees not contain TSC A c 10 RESULTS (Continued) 8. Pathology (Continued) No compound-related abnormalities were detected the livers of the dogs receiving 500 and 750 ppi DISCUSSION In the previously-reported ninety-day feeding study in rats and d o g s f i f l B l H B the lowest level fed, 100 ppm, was considered the "no- e f f e c ^ ^ ^ ^ ^ The other two levels, 500-1,000 ppm and 2,500-5,000 ppm, did produce changes in both rats and dogs. In rats, this consisted of hematological changes at 2,500-5,000 ppm, increased liver and kidney weights at 500-1,000 ppm and 2,500-5,000 ppm levels, and pale yellowish livers in some male rats at the mid and highest levels; histologic changes were observed in only the livers from the highest level, 2,500 5,000 ppm. In dogs, plasma cholesterc^aric^lkaline phosphatase were elevated in those fed 2,500-5,000 suggesting liver damage. Liver weights were increased a^th^mrodle and highest dietary levels; histologic changes were observed in only the livers of dogs receiving the highest dietary level.- The present study confirms the results obtained earlier. There does not appear to be a "no-effect" level in this present study, since increased liver and kidney weights were observed at the lowest dietary level in rats and since increased alkaline phosphatase activity and increased liver weights were observed in the lowest level in the dogs. Thus, the results of this ninety-day feeding study and y ^ ^ ^ ^ ^ t h e previous one support only 100 ppm as a "no-effect" level SUMMARY lhas been fed to rats and dogs for ninety days at dietary levers of 0, 500, 750, and 2,500 ppm. No nutritional or clinical signs of toxicity were observed in rats or dogs on any of the test levels. t ^jsreceiving the intermediate and highest dietary levels of J H H s h o w e d lower erythrocyte counts, hemoglobin concentrations "and hematocrits than those of the controls; those receiving the highest dietary level also showed a slightly higher incidence of hematuria and .proteinuria. Male rats receiving the two higher dietary levels of 'jjHHflHHftshowed slightly larger liver and kidney weights and higher ^S^^^^^^fyweight ratios than did the controls or other test group; all female test rats had higher kidney weights and kidney/body weight ratios whereas the fem^Le rats on the 'highest dietary level also had greater liver weights and liver/body weight ratios. Of all che organs examined histologically, on^^tl^^^wers, and only those from the male animals receiving 2, 500 ppmaH|^HU^||| showed changes, and these were considered to be reversible. Dogs receiving the highest dietary level o f ^ H ^ H H ^ a d lower erythrocyte counts, lower hemoglobin concentrations, and hanatocrit values Cbm psny Sanitized. Does not contain T S C A G G 11 SUMMARY (Continued) than did the controls. Alkaline phosphatase activities were elevated in the dogs in all test groups, wherea^cholesterol values were higher in dogs receiving 750 and 2,500 ppnfljjHj|m|Hp The livers of the dogs from all three test groups were heaviertnai^tnose from the control dogs. The liver was the only organ of those examined that exhibited histolfogic changes and this was confined to those coming from the highest level group. Thus, the results of this ninety-day feeding study and those of the revious one support only 100 ppm as a "no-effect" level for i ir- P-- _4i ft Pompany Sanitized. Does not contain T S C CBi AVERAGE BODY WEIGHT (GRAMS) U W U I vvI_I\J I I i ' v-' i 2 3 4 5 6 7 8 9 IO II 12 13 T I f\/i r M f j r r CrV3mpany Sanitized. Does not contain TSCA CBS TABLE I } I AVERAGE BODY WEIGHTS AND AVERAGE WEIGHT GAINS OF MALE RATS FED VARIOUS LEVELS OF Days on Test 0 7 14 21 28 35 42 49 56 63 70 77 84 91 Group I Control Group II 500 ppm Group III 750 ppm Average Body Weight (gm) 70 69 69 123 120 119 179 174 172 233 227 229 266 280 267 321 323 320 358 358 358 388 390 396 411 413 423 432 431 441 452 451 461 472 470 478 487 482 -- 492 500 497 510 Group IV 2,500 ppm 68 117 168 221 267 310 345 377 404 423 448 471 489 495 0- 7 7-14 14-21 21-28 28-35 35-42 42-49 49-56 56-63 63-70 70-77 77-84 84-91 Average Weight Gain (gm) 53 51 50 56 54 53 54 53 57 33 53 38 55 43 53 37 35 38 30 32 38 23 23 27 21 18 18 20 20 20 20 19 17 15 12 14 13 15 18 49 51 53 46 33 35 32 27 19 2.5 23 18 6 TABLE II AVERAGE BODY WEIGHTS AND AVERAGE WEIj [T GAINS FEMALE RATS FED VARIOUS LEVELS OF Days on Test 0 7 14 21 28 35 42 49 56 63 70 77 84 91 Group I Control Group II 500 ppm GroupEli i Group IV 750 ppm 2,500 ppm Average Body Weight (gm) 69 70 113 114 147 148 170 173 189 194 205 208 220 225 238 236 250 247 250 253 259 260 270 268 279 276 282 277 70 110 149 175 196 214 228 244 257 262 273 286 290 295 70 112 150 176 195 208 217 236 246 247 261 267 275 276 0- 7 7-14 14-21 21-28 28-35 35-42 42-49 49-56 56-63 63-70 70-77 77-84 84-91 Average Weight Gain (gm) 44 35 22 19 16 15 18 12 0 9 11 9 3 44 40 34 39 25 26 21 21 14 18 17 14 11 16 11 13 65 7 . 11 8 13 84 15 42 38 26 19 13 9 19 10 1 14 6 8 1 not contain TSCA CBi Sanitized. Does Company TABLE III AVERAGE DAILY FOOD CONSUMPTION AND FOOL :f f i c i e n ( ' . O F MALE RATS FED VARIOUS LEVELS OFi 1/ Days on Test 0- 7 7-14 14-21 21-28 28-35 35-42 42-49 49-56 56-63 63-70 70-77 77-84 84-91 Group 1 Control Group 11 500 ppm Group III " Group IV 750 ppm 2,500 ppm Average Daily Food Consumption (gm) 14.9 20.1 19.4 23.3 25.0 25.4 24.4 25.6 25.6 24.4 24.5 25.0 23.8 14.8 18.4 20.7 24.3 22.1 25.8 25.2 25.7 25.5 24.9 24.5 24.7 23.1 14.0 18.5 21.1 23.3 25.5 25.8 26.1 25.8 25.8 25.9 25.9 26.3 25.4 14.1 18.2 20.1 23.1 24.8 25.4 25.6 26.1 24.2 26.1 27.9 27.4 25.4 0- 7 7-14 14-21 21-28 28-35 35-42 42-49 49-56 56-63 63-70 70-77 77-84 84-91 Gram Weight Gain/Gram Food Consumed . 0.51 0.40 0.40 0.20 0.31 0.21 0.18 0.13 0.11 0.12 0.11' 0.09 0.08 0.50 0.42 0.37 0.31 0.28 0.19 0.18 0.13 0.10 0.11 0.11 0.07 0.09 0.51 0.41 0.38 0.23 0.30 0.21 0.20 0.15 0.10 0.11 0.09 0.08 0.10 0.49 0.40 0.38 0.28 0.25 0.19 0.18 0.15 0.11 0.14 0.12 0.09 0.03 not contain TSCA CB? C o m p any Sanilizod. Does TABLE IV AVERAGE DAILY FOOD CONSUMPTION AND FOOD^EFFICIEl OF FEMALE RATS FED VARIOUS LEVELS OF| Days on Test 0- 7 7-14 14-21 21-28 28-35 35-42 42-49 49-56 56-63 63-70 70-77 77-84 84-91 Group I Control Group II 500 ppm Group I I I 750 ppm Group IV 2,500 ppm Average Daily Food Consumption (gm) 13.5 16.1 12.9 17.0 17.6 17.8 18.2 18.8 21.2 16.2 18.2 18.5 17.0 14.0 16.9 16.8 18.2 16.9 19.1 17.7 20.4 20.6 17.6 19.2 19.0 16.9 13.0 16.0 15.6 17.3 17.2 18.1 18.8 19.1 18.3 17.3 20.2 18.6 17.9 13.1 15.9 14.7 17.4 16.9 17.3 18.1 18.9 18.2 17.3 18.9 18.3 16.5 0- 7 7-14 14-21 21-28 28-35 35-42 42-49 49-56 56-63 63-70 70-77 77-84 84-91 Gram Weight Gain/Gram Food Consumed 0.46 0.31 0.24 0.16 0.13 0.12 0.14 0.09 - 0.08 0.08 0.07 0.02 0.45 0.29 0.21 0.16 0.12 0.13 0.09 0.08 0.04 0.06 0.06 0.06 0.01 0.44 0.35 0.24 0.17 0.15 0.11 0.12 0.10 0.04 0.09 , 0.09 0.03 0.04 0.46 0.34 0.25 0.16 0.11 0.08 0.15 0.08 0.01 0.11 0.05 0.06 0.01 Company Sanitized. Does not contain T S C A CBI TABLE V AVERAGE WEIGHT GAIN, FOOD CONSUMPTION, AND FOOD EFFICIENCY DATA, CALCULATED AT APPROXIMATELY MONTHLY INTERVALS, OF MALE AND FEMALE RATS FED VARIOUS LEVELS OFl Group I (Control) Days on Test 0-28 28-56 56-91 Total Weight Gain (gm) 196 145 89 430 MALES Food Consumption (gm) 544 703 863 2110 Food Efficiency 0.36 0.21 0.10 0.20 II (500 >m 0-28 28-56 56-91 Total 211 133 84 428 548 692 859 2099 0.38 0.19 0.10 0.20 III (750 im 0-28 28-56 56-91 Total 198 156 87 441 539 723 905 2167 0.37 0.22 0.10 0.20 IV (2500 ppm 0 0-28 28-56 56-91 Total 199 137 91 427 529 713 917 2159 0.38 0.19 0.10 0.20 Weight Gain (gm) 120 61 32 213 FEMALES Food Consumption (gm) 417 507 637 1561 Food Efficiency 0.29 0.12 0.05 0.14 124 461 0.27 53 519 0.10 30 654 0.05 207 1634 0.13 126 434 0.29 61 512 0.12 38 647 0.06 225 1593 0.14 125 428 0.29 51 499 0.10 30 625 0.05 206 1552 0.13 Company Sanitized. Does not contain T S C A CBI TABLE VI AVERAGE DAILY INTAKE OF Average Dose in Milligrams/Kilogram/Day Days on Test Group I Control 0- 7 7-14 14-21 21-28 28-35 35-42 42-49 49-56 56-63 63-70 70-77 77-84 84-91 - - - - - - - - 0- 7 7-14 14-21 21-28 28-35 35-42 42-49 49-56 56-63 63-70 70-77 77-84 84-91 - _' - - - - - - Group II 500 ppm MALES 78 63 52 48 37 38 34 32 30 28 27 ... 26 24 Group III 750 ppm 112 95 79 70 65 57 52 47 . 45 43 41 41 38 . FEMALES 76 65 52 50 42 44 38 42 41 34 36 35 31 108 92 72 70 63 61 60 57 53 48 53 49 46 Group IV 2,500 ppr 382 319 259 237 215 194 177 167 144 149 152 142 129 361 303 226 234 209 204 200 196 185 170 1-79 169 150 m Company Sanitized. Does not contain TSCA CBiT TABLE VII SUMMARY OF HEMATOLOGIC MEASUREMENTS ON RATS FED '(DR THREE MONTHS -- Erythrocytes x 106/mm3 Hemoglobin gm/100 ml Hematocrit % Leucocytes x 103/mni3 O o 3 w Heutrophils % Lymphocytes % ppm in Diet 0 500 750 2,500 0 500 750 2,500 0 500 750 2, 500 0 . 500 750 2, 500 0 500 750 2,500 0 500 750 2,500 1 5.98 _ 5.64 5.58 14.9 - 13.9 13.3 43 - 39 38 13.4 - 12.9 13.7 22 - 26 25 75 - 72 73 MALES Months on Test 2 6.14 5.65 5.39 4.92 15.6 15.6 14.5 13.7 45 43 42 39 13.2 12.4 14.1 15.9 19 22 21 19 77 75 75 79 3 6.38 5. 70 6.12 5.27 16.7 15.2 15.6 15.5 49 43 45 44 12.6 10.7 12.3 14.5 18 23 22 21 79 75 75 76 1 5.87 - 5.86 5.94 15; 4 - 15.2 14; 9 44 - 41 41 10.8 - 10.2 14.4 20' - 21 19: 11 - 76 78 FEMALES Months on Test 2 4.65 5.38 4.33 4.18 13.5 15.1 12.1 11.8 37 43 33 32 10.5 9V6 10.6 14.2 22 17 21 ' 19 74 80 75 77 3 6.04 5.66 5.93 5.17 16.6 15.9 16.2 15.3 46 44 45 42 7.1 7.3 "&.9 10.1 20 20 19 2 76 77 78 75 Sanitized. Does not contain TSCACBI f ^ ^ J la n jzed. Doesnocontain TSCACB| SUMMARY OF HEMATOLOGIC MEASUREMENTS ON RATS FED, Monocytes % Eosinophils % Basophils % Atypical Cells % Nucleated RBC's per 100 WBC's ppm in Diet 0 500 750 2,500 '0 500 750 2,500 0 500 750 2,500 0 500 750 2,500 0 500 750 2,500 MALES Months on Test 12 3 1.0 J - 1.5 1.4 2.0 - 1.1 1.4 0.1 - 0.1 0 0.9 0.6 0.4 0.5 2.4 2.3 3.2 2.2 0 0.1 0.1 0 0.1 0.6 0.1 0.5 2.5 2.1 2.8 2.9 0 0.1 0 0.1 000 - 00 000 000 0 0.1 0 _ 00 0 0.1 0 000 FiOR THREE MONTHS FEMALES Months on Test 12 0.3 1.0 - 0.1 1.0 0.9 0.9 1.2 2.5 4.7 - 2.7 2.2 3.2 2.1 3.2 00 -0 0.1 0.1 0 0.1 0: 0 -0 00 O' 0 00 -0 00 0: 0 3 0.5 0.7 0.5 0.8 3.5 2.7 2.4 3.4 0.1 0 0 0.1 0 0 0 0 0 0 0 0 N 00 empany Sanilteed Ooes not soniain TSCACBi TABLE VIII SUMMARY OF URINALYSIS DATA ON RATS FED, Volume ml/24 hrs. Osmolality mO s/L Creatinine mg/24 hrs. Blood Number Eositive Sugar Number Abnormal Bilirubin Number Positive ppm in Diet 0 500 750 2,500 0 500 750 2,500 0 500 750 2,500 0 500 750 2,500 0 500 750 2,500 0 500 750 2,500 MALES Months on Test 12 _ 15 15 1349 - 1578 1760 9.2 - 9.5 9.3 0 0 1 0 - 0 0 0 _ 0 0 19 22 18 20 1696 1818 1845 1757 17.4 16.7 17.8 16.7 1 0 0 2 0 0. 0 0 0 '0 0 0 3 17 16 18 21 1857 2012 1860 1711 15.2 15. 9 17.0 16.7 0 0 0 1 0 0 0 0 0 0 0 0 1) Number -H- or greater by Clinitest IFOWR THREE MONTHS 1 13 - 12 13 1590 - 1849 1783 7.1 7.1 7.4 0 0 1 0 0 0 0 - 0 0 FEMALES Months on Test 2 19 15 15 18 1473 1763 1792 1644 11.8 10.0 10.8 10.3 1 1 0 3 0 0 0 0 0 0 0 0 3 14 12 15 13 1619 1926 1852 1842 9.0 8.2 9.5 8.6 0 0 0 1 0 0 0 0 0 0 0 0  TABLE VIII (Continued) SUMMARY OF URINALYSIS DATA ON RATS FE FOR THREE MONTHS ppm in Diet Protein Number Abnormal ' 0 500 750 2,500 Urobilinogen 0 500 750 Erythrocytes per hpf 2,500 0 500 750 2, 500 C? 0 x3z Leucocytes per hpf 1 OS D3> Epithelial Cells NS per hpf <p9. ao<(9 Casts per lpf 0 500 750 2,500 0 500 750 2,500 0 500 750 2,500 MALES Months on Test 12 00 _0 00 01 0.4 1.0 - 1.0 0.6 1.0 0.5 1.0 0 0a) -0 o , 0 ,, , 0a) 0a)b) 0-1 0-1 - 0-1 0-1 0-1 0-1 0-1 00 -0 00 0 0-1 00 -0 00 00 3 0 0 0 2 0.6 0.8 0.7 0.7 0 0 o oa) 0-1 0-1 0-1 0-1 0-1 0-1 0-1 0 0 0 0 0 2) Number ++ or greater by Albustix a) 1/10 hematuria b) 1/10 with positive test for blood examined separately c) 2/10 with positive test for blood examined separately 1 0 0 0 0.7 0.7 0.6 0 " 0-1 - 0-1 0-3 0 0 0 0 0 0 FEMALES Months on Test 2 0 0 0 0 1.0 1.0 1.0 1.0 0 0 ^N oa ) c ) 0-1 0-1 0-1 0-1 0-1 0-1 0 0 0 0 0 0 3 1 2 1 3 0.8 0.9 0.8 0.8 0 0 0^ la) 0-1 0-3 0-1 0-1 0-1 0-1 0-1 0-1 0 0 0 0 noi contata TSCAC8 o 91 <fn 3. W CCDU o o(0D5 OJ3 0 H Ci O ffi TABLE TX SUMMARY OF BIOCHEMICAL MEASUREMENTS ON RATS'FED, Alkaline Phosphatase Bessey Units Transaminase Reitman-Frankel Units Bilirubin mg % ppm in Diet 0 500 750 2,500 0 500 750 2, 500 0 500 750 2,500 ________ MALES Months on Test 12 3 44 38 32 47 38 33 41 33 24 18 12 8 18 18 1.1 10 12 1.7 9 18 0.6 0.6 1.5 0.3 0.5 1.4 0.4 pFOR THREE MONTHS FEMALES Months on Test 12 3 35 22 19 33 23 17 26 20 14 14 19 14 15 17 1.0 18 21 0.3 12 15 0.5 0.9 0.4 0.5 1.2 0.4 0.4 Group (Control) II (500 ppm TABLE X a v e r a g e ORGAN WEIGHTS IN GRAMS OF MALE AND FEMALE RATS FED VARIOUS LEVELS OfJ Final Body- Weight Brain Heart Lungs Liver Spleen Kidney Testis Stomach Adrenal MALES 500 2.19 1.44 2.39 17.08 0.77 3.46 3.36 2.04 0.058 505 2.24 1.50 2.40 17.95 0.71 3.78 3.27 1.90 0.053 Pituitary 0.012 0.014 III (750 ppm 524 3 3IV (2,500 ppm 495 ao 3 XI w 286 (Control) CO II (500 288 2.25 1.52 2.47 21.46 0.70 2.19 1.45 2.42 22.00 0.69 2.06 0.91 1.84 FEMALES 10.72 0.57 2.02 0.96 1.76 11.39 0.49 4. 30 4.45 2.13 2.66 3.22 2.06 3.31 2.03 - 1.43 - 1.53 0.058 0.059 0.074 0.076 0.016 0.014 0.017 0.019 anitized. Doss not contain TSCA III :750 ppm 306 2.08 0.97 1.86 11.76 0.53 2.87 - 1.65 0.078 0.016 IV (2,500 a !i0 276 2.06 0.97 1.85 13,24 0.53 3.04 1.46 0.089 0.019 TABLE XI .1 SUMMARY OF ORGAN/BODY WEIGHT RATIOS OF MALE AND FEMALE RATS FED VARIOUS LEVELS OFl Group (Control) II (500 ppm Brain 0.44 0.44 Heart 0.29 0.30 Lungs 0.48 0.48 Liver 3.41 3.55 % of Body W;ight Spleen Kidney Testis Stomach MALES 0.15 0.69 0. 67 0.41 0.14 0.75 0.65 0.38 Adrenal 0.012 0.010 Pituitary 0.002 0.002 III :750 pprfi IV (2,500 ppm 0.43 0.29 0.47 4.10 0.44 0.29 0.49 4.44 0.13 0.14 0.82 0.90 0.61 0.67 0.39 0.41 0.011 0.011 0.003 0.002 ao 'S33 FEMALES S3 I (Control) 0.72 0.32 0.64 3.75 0.20 0. 74 - 0.50 0.025 0.005 i93f8) w II [500 ppm 0.70 0.33 0.61 3.95 0.17 0.92 - 0.53 0.026 0.006 <0 a, a oSO III (750 ppm 0. 68 0.32 0.61 3.84 0.17 0.94 - 0.54 0.025 0.005 IV (2,500 ppm 0.75 0.35 0.67 4.80 0.19 1.10 . 0.53 0.032 0.006 r.ct contain TSCA CB6 % TABLE XII HASKELL ff COMPOUND AMIRAL l> CROUP K treatm ent days p o s t --t k n a t m e n t " d a y s DIPT/DOSE VALUE I N I T I A L WEIGHT LOW W E IG H T F I N A L V,EIGHT SIX U l E 0 / S ACRI FT CFU H l STGPATHQLUGY TO-OAT6 SUMMARY _ RAT PAGE - 1 JULY 10, 1972 . 7558 01 51 7561 1 91 62 454 H 63 527 M S 66 490 H S 67 472 H S 67 492 M 69 501 H 73 502 M 77 562 M 70 497 H 7555 07 91 7575 07 91 7501 07 91 7550 07 91 7544 07 91 2600 2500 2500 2500 2500 2500 82 67 62 66 6 6 66 67 505 M s 406 M 500 M S 304 M S 430 M S 510 H s 434 M E Y E NORMAL MORPHOLOGIC S T R U C T U R E S F X L i R U i T A L $ A C k IMAI. GLAND NORMAL MOR P H U L H i l C S T R U C T UR 1 5 i i . i A I M NORMAL M CP.P HIII .UGIC S T R U C T U R E S MEDULLA (V.LONGATA NORMAL MGRPHOLOGl i. 5* f J\ U C 1UH LS S C I A T I C NCRVC NORMAL MORPHOLOGIC ST RUC TUNES S K IN NORMAL H O R P I U 'U iC I C S T R U C T U R E S SK IN ORGAN; M IS S ! N G MAMMARY GL AND NORMAL K O R P r i O l D G i C ST RUCTJTES MA.".; 1A R Y GL AND 1! R GAN ' M1S S 1NG ---NE MARROW NORMAL MUA PI IOLUG I C STRU C T URL- S S P L E E N NORMAL MORPHGL L'G I G S T R U C T U R E LYMPH NODE n o r m a l MORPH!1 UC 1C S T R U C TONI'S LYMPH Is Hit ORGAN M I S S I N G S K E L E T A L M U SC L E NORMAL MUNPHUI TlG IC STRUCTURES T k APHE'A NON MAL Mi T PI m il GG I G S T R u C T U K cr LUNG NURMAL MORPHOLOGIC S T R U C T U R ES LUNG IN T i R STJ T A L PNEUMONIA H EA RT NORMAL M O P P H C LIV .IC S T R U C T U R E S AS. AT A NORMAL M UKPHHL.T GIC S T R U C T U R E S S A L I V A R Y L A N D NORMAL M ORPHOLO GIC S TRUCTURIiS L I V E R NORMAL MORPHOLOGIC S T R U C T U R E S L i V E K G-IAN'JLOMA L IV E R CLOUDY SWELLING L IV E R HYALIN E DROPLET DEGENERATION 0 00000 0000 00 a 0u 0 0000 0 0 0 00 0 000 00 J 0000 0u00 U 0 0 u 0 0 l) 0 0 0 0 0 0 0 0 0 0 0 0 . 0 t) 0 0 0 0 0 0 0 0 0 0 u 0 0 D G .0 00 0 0 0 0 0 0 0 0 G 0 D ') 0 X 0 00 XXXXX X XXX X X XX 0 0 0 00 0 0 00 0 U0 00 0 0 0 0 0 0 0 0 0 0 0 0 J 0 (1 J . 0 00000000u0 0 0 0 u0 X 0 0 0 0 0 0 0 0 0 (J 0 0 0 0 0 0 0 0 0 0 0 0 0 0 u 0 u u 0 0 00 000 0 0 U0 0 0 + 000 0 00 00 000 00 00 0 0 00 0 0 0000 00 0 00 00a 0 0 00000 0000 0 0 0 0 00 0 00000 00000 0 npany S a n i t i z e d , Does not contain TSGA Cffi LIVER CYTOPLASMIC LIPID DROPLET ALT ++ TABLE XII (Continued) A j1 ' " 1 * ; ANIMAL K L I V E R HY P E R T KOPMY PANCREAS llllK llC STRUCTURES ' NORMAL MORPH ESOPHAGUS NURMAL m o r p h o l o g i c s t r u c t USES STOMACH NORMAL MORPHOLOGIC S T R U C T U R CS DUODENUM NORMAL MORPHOLOGIC STRU CTU RES CCfO-CLOUMN NNOOPRMMAALL MMOORRPPHHOOLLOOGGIICC SSTTRRUUCCTTUURREESS K I D K'l Y NORMAL MORPMOIOGIC S T R U C T U R E S USTRRI NUCATR YUK GI SL ADDER NORMAL MORPHOLOGI C pro state aho s e m in a l v e s ic l e normal MOKPHULUG 1C STRU C TU R ES TE S T IS MIUMAI. MORPHOLOGIC STRUCTURE s E P I ' M D Y M I S NORMAL MORPHOLOGIC STP.UC TIJRES CUTTUL RI HPISS , NORMAL MORPHOLOGIC STRU ETPAUl Cl OT PUIRA.ENS TUBE NORMAL MORPHOLOGI C S OVARY NUi-AAL MORPHOLOGIC STRUCTURES P IT U IT A R Y GLANO NORMAL MORPMOIOGIC STRUC1URLG KADURC'T-MUAXLf S GLANO NORMAL M U R P I I O U H . I C ST TP.HUCY R1UORI DE S GLANO NORMAL MORPHOLOGI C ST THYROID GLANO ORGAN M ISSIN G CP A SRTARTUHCYTRIOJKI DI SGLAND NORMAL MORPMOLOGI PA RATHYROID r,l AND o r g a n m i s s i n g THYMUS No r m a l MORPHLU OGIC STRUCTURE s PAGE - 1-/1 JULY 10, 1972 7558 7561 7560 000 000 000 00 0 000 000 0 0 7574 7565 7539 000 000 000 000 000 000 0 00 7541 7536 7568 0 00 000 000 000 000 0 00 00 7578 0 0 0 0 0 0 0 7546 7555 7575 + +, 000 000 00 00 000 0 0 000 7501 7550 + 00 00 00 00 00 00 0 75 Vt 4- 0 0 0 o o 0 0 0000 000 000 0 0 0 0 0 u 0 0 0 0 0 0 0 0 0 0 Q0 0 0 0 0060600000 0 0 0 00 0 0 0 0 0 0 0 0 0 0 0 u 0 0 0 0 00000p0000 00 0 00 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 XXXXXX XX XXX 0 0 0 X X X X X X- X 0 0 X X X X X X 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 u 0 osnpany Sanitized, Does noi esntain TSGA CS| TABLE XII-A HASKELL COMPOUND v * AN MAL il GROUP .7 TREATMENT IJAYS POST-TREATMENT DAYS O IET/D U SE VALUE IN IT IA L HEIGHT LOW W EIGHT FlfJA L WEIGHT SEX QIEIJ/SACh IEIC ED HIS TOP ATHOLOGY TO-DATE SUMMARY RAT PAGE - 2 JULY 10. 1972 7 s a ;-, . 7 6 7.3 07 07 91 91 75e4 07 91 2500 25 Jr 2500 70 u 7A 7543 07 91 2500 79 633 M S 519 M S 523 M S 512 IT S 7617 02 92 49 243 F S 7623 02 92 62 268 F S 7618 02 92 63 270 F S 7621 C2 92 70 333 F S 7624 7507 02 02 92 92 72 72 273 275 FF SS 7595 02 92 72 291 F S 7633 7634 02 02 92 92 73 75 281 302 F rSs 7592 02 92 7619 08 92 759 0 9 2500 250' 80 60 6 322 242 25 pF s3 E Y E NORMAL- MORPH OLOGIC S T R U C T U R E S E X O P . B I T A L L A C R I M A L GLAND NORMAL MUR PHOLOGIC li'U C IU : ES Di'. A I N NU '.MAL MORPH OLOGIC S T R U C T U R E S m e d u l l a o b l o n g a t a n o r m a l mup. p h o l o g i C S T ,LUC TUNES S C I A T I C NERVE NORMAL MORPHOLOGIC ST RUC H I R E S S K I N NORMAL M UR PII ULO G1C S T R U C T U R E S SKIM ORGAN M IS S IN G MAMMARY GLANU NORMAL M ORPH OLOGIC ST RIJCTURES <'C3> 2 <5 m a m m a r y g l a n d o r g a n m i s s i ir, 15ONE MARROW NORMAL MORPM'.ILUG I C ST RU crup.es S P L E E N NORMAL M ORPH OLOGIC S T R U C T U R E .<3 S Sanitized. Does LYMPH NUDE NORMAL MUKPHULOGIC STRUC TORES LYMPH MODE ORGAN M I S S I N G S K E L E T A L MUSCLE NORMAL MORPHOLOGIC STRUCTURES TRACHEA NUKMAL MORPHOLOGIC STRUCTUR ES LUNG NORMAL M ORPH LUGIC S T R U C T U R E S 3 UJNG IN T E R S T IT IA L PNEUMONIA O HEART NORMAL MORPHOLOGIC S T R U C T U R ES O A O R T A NORMAL MORPHOLOGIC S T R U C T U R ES O --S A L I V A R Y GLAND NORMAL M ORPH OLOGIC S gTRUCTURES _ ^ I V E R NORMAL MORPHOLOGIC S T R U C T U R E S ( 1 V E R GRANULOMA SS>TII V E R CLOUDY S W E L L IN G 1L 1i V E R H Y A L I N E D R O P L E T D E G E N E R A T IO N 03 LIVER CYTOPLASMIC LPID DKOPLET ALT 00000000 0000 00 00 00 0000 0 000 0 0 U 000 0 0 0 0 0 0 0 0 0 u ") 00 00 000 00 0 0 0 0 u0 0 0 0 0 0 00 0 00 J 0 0 u 0000000000u 0000 00 000 0 0 0 0u XXX X ): 00000000000 0 0uu 0000 000 000 0 0 0 0 0 00 0 0000 0 u 0 000 XX 0000 000000 00 00u 00 0 0 00 0 00 0 0 0 0 0 0000000000 000 00 0000 000000 00 0 00 0000000000 000 00 0000000000 00000 0 000000 00 000 ++ T7 .TABLE XII-a (Continued) ANIMAL # LIVER h y p e r t r o p h y ' PANCREAS OL'JGIC STRUCTURES NORMAL MORPH ESOPHAGUS NORMAL MORPHOLOGIC ST RU CT ORES STOMACH NORMAL MORPHOLOGIC S T k U C T U R ES DUODENUM NORMAL M ORPH OLOGIC S T R U C T U RES COLON NORMAL MORPHOLOGIC S T R U C T U R E S CECUM NORMAL MORPHOLOGIC S T R U C T U R E S NIUNCY NUPMAL NORPOOLUGIC ST R U C T U R E UKIN A RY GLADDER NOKMAl M ORPHO LOG IC S TXUCTURES PR O S T A T E AND S E M IN A L V F S I CL E NORMAL MURPHCLOG IC S T R U C T U R L S T E S T I G NORMAL MORPHOLOGIC S T R U C T U R E S E P ID ID Y M IS norm al MORPHOLOGIC STRUC TORE S UcTt uEpR.Ui Ss ' NOKMAL MORPHOLOGIC STRU F T A r. LLiLCOT PIAN UP.r s TU3E NORMAL MORPHOLOGIC S OVARY NORMAL M U A P H O Lc G IC S T R U C T U R E S P I T U I T A R Y GLANU NORMAL MORPH'UOG C STRUCTURES ADP NAI. GLAND NORMAL M ORPH OLOGIC ST ROC T I W S T H Y R O ID GLAND NOKMAL MORPHOLOGIC ST RUC IURES t h y r o id gland organ m is s in g P A R A T H Y R O ID GLANU NORMAL MUHPH0LUG1 C STRUCTURES P A R A TH Y R O ID GLAND ORGAN M IS S IN G TRHYMUS NORMAL MOI.' PH CLOG I C S T R U C T U R E P A C E - 2 --A JU LY 10*..1972 7543 + 0 0 0 0 0 7573 7584 + 7548 t 00 00 000 000 000 0 U0 7617 0 0 0 0 0 0 7623 0 0 0 0 0 0 7618 7621 7624 7587 0000 00 U0 0000 0000 0 0' 0 0000 7595 7633 00 00 00 U0 00 00 7634 7592 00 00 00 U0 U0 0 7619 0 U 0 J 0 c 7 59 A O 0 O Oc o U 0 0 U 0, 0 0 0 U 0 0 0 0 U 0 0 0 0 0 0 0 0 0 0 J u 0 0 U 0 0 U0 0 0 0 0 0 U 0 0 0 U U a 1} U 00 0 00 U 0 0 0 U 0 0 0 G 0 0 0 0 O 0 0 0 0 0 0 0 U 0 0 0 0 . U O 00 00 0 0 0 00 0 0 U0 0 O 0 000 0 X X X X X X X X X X. X 00 XXXXX X. X X X X XXXX 0 0 U0 0 0 0 0 0 U0 U 0 0 0 O O en omparey Sanitized. Does not contain TSCA TABLE XII-B HASKELL COMPOUND" ANIMAL It GROUP 1! TREATMENT DAYS POST-TREATMENT DAYS DIET/DOSE VALUE IN IT IA L WEIGHT LOW vJFIGHT FIN A L WEIGHT SI: K D llO /S A C R IF IC E D H I S T U P A T H O L U G Y T O --O A T S SU MMA RY - R A T PAGE - 3 JU LY ID ,. 197 2 7 G'32 00 92 2500 67 303 F S 760203 92 2500 71 . 260 F S 76Q3 OC 92 2500 73 260 F S 7613 08 92 2500 74 268 F S 7590 08 92 2500 75 319 F S 7589 03 92 2 500 76 274 F S 7600 08 92 2500 77 291 F S 7614 08 92 2500 80 250 F S 7542 03 96 500 63 433 K S 7572 03 7547 03 96 7559 03 96 500 500 500 68 68 68 453 M S 477 M S 521 H S 7563 03 96 763 03 *36 756* 03 96 750 2 03 96 500 3U 500 5 0 0 70 73 70 UG 547 M S 50 7 M s 36b M s 522 M S E Y E NORMAL MCJRPHULUGIC S T R U C T U R E S E X O K t U T A L L A C K I HAL GLAND NORMAL MOR PHOLOGIC STRUCTURES S R .U N NORMAL MORPHOLOGIC S T R U C T U R E S MEDULLA fJCLONGATA NORMAL MORPHOLOGI C STRUCTURES S C I A T I C NERVE NORMAL M ORPH OLOGIC ST KUCTURLS S K I N NORMAL MORPHOLOGIC S T R U C T U R E S S K IN OKG AN .MISSING MAMMARY GLAND NORMAL MORPHULOG1C ST RUCTURCS MAMMARY GLAND ORGAN M I S S I N G GONE HARROW NORMAL M ORPH OLOGIC ST RU . C T O R ES S P L E E N NORMAL MOKPMULUG1 C S T R U C T U R E S LYMPH NODE NORMAL MORPHOLOGIC STRUC TUMES LYMPH MODE ORGAN M I S S I N G S K .- L E T A L MUSCLE NORMAL H U K P H O IO G IC STRUCTURES TR A C H EA NORMAL M OKPHULUGIC S T K U C IU R FS LUNG NORMAL MORPHOLOGIC S T R U C T U R E S LONG I N I E R S T I T I A L PNEUMONIA HEART NORMAL MORPHOLOGIC S T R U C T U R ES AORTA NORMAL MORPHOLOGIC S T R U C T U R E S S A L I V A R Y GLAND NORMAL MORPHOLOGIC S TRUCTURES L IV E R . NORMAL MORPHOLOGIC ST R U C T U R ES L I V E R GRANULOMA . .. L IV E R CLOUDY SWELLING L IV E R HYALINE DROPLET DEGENERATION 0 0 00 00 0 0 00000000 0 0 0 0 0 0 .0 0 0 0 00 00 0 0 00 0000 00 0 0, 0 0 0 0 0 0 0000000 X 0 0 00 00 00 0 0 0 0 .0 , 0 0 0 00 00 00 0 0 0 00 0 000 0 0 0 0 0 0 0 0 0 0 0 + c c 0 0 0 0 0 0 0 0 0 0 0 0 0 0 00 00 0 0 0 0 0 0 0 0 0 0 0 00 00 'Company Sanitized. Does not contain TSCA CB LIVER CYTOPLASMIC LIPID DROPLET ALT ( TABLE XII-B (Continued) HISTOPATHQLOGY TO-DATE SUMMARY - RAT PAGE - 3--A HASKELl. COMPOUND ANIM AL fl JULY 10, 19.72. 7 6 3 2 7 6 0 2 7 6 0 3 7 6 1 3 7 5 9 0 7 5 8 9 7 6 0 0 77 66 11 44 7 5 4 2 7 5 7 2 7 5 4 7 7 5 5 9 7 5 6 3 7 5 0 3 7 5 6 4 7 5 0 2 LIV ER HYPERTROPHY PANCREAS NURMAL MORPH O LU G IC . STRUCTURES ESOPHAGUS NORMAL M ORPH OLOGIC STRU.CT UNES S I C M A L H NORMAL M ORPH OLOGIC S T R U C T U R r. S DUODENUM NORMAL M0KPHU1.UGIC S T R U C T U P LS COLON NORMAL MORPHOLOGIC S T R U C T U R E S CLCUM NORMAL MORPH OLOGIC S T R U C T U R E S 0 0 0 0 0 00 0 0 0 0 0 00 0 0 0 0 0 00 0 0 0 0 0 00 0 0 0 0 0 00 0 0 0 0 0 0c 0 0 0 0 0 0 K ID N E Y NORMAL MORPHOLOGIC S T R U C T U R E $ O' l l M A K Y IM ADDER NORMAL MO" P HDL CIG I C gTII OC T UK 1. S g ' f l S T A T P AND S LM I NAL V E S I C L E NORMAL *<M0RPH0L 00 1C S TP UC T URE S OBEST I S NORMAL MORPHOLOGIC S T P U C T U K E S g P l D I O Y M l S MMP MAL MORPHOLOGIC S I MIC ftcn-.ts Q JTFi:U -S N O R M A L M O K PM U .O V il C > T K \J Tl/y. ( s C f A L U ' P l A N U JU i: NORMAL W K P M U lU G t C S S tructures 3U V AR Y Nu H. MAL MORPHOLOGIC S T KUCTUR c S 2>' 1 T U I T A E Y GL AND n l ih m a l MORPHOLOGIC O S TRUC I U'<! s A i ;P T M A L C IA N O NURMAL MORPHOLOG I C ST JP.UCTURPS 5 ' T I I Y P . U l i ) ULANI) NORMAL MOP PHOL U IJ I C ST -jl'.UL T URI. S CO T H Y R O I D CLAMO ORGAN M I S S I N G >0 O CD p al.A IHYP.O I 0 C S TRUCI U PS PARATHYROID GLAND GLAND NORMAL MOKPHOLfTGI URGAN M IS S IN G THYMUS NORMAL MORPHULUGIC S T RU CT U R E 0 0 0 0 0 00 0 0U00 0 00 0 0 0 0 0 0 0O 0 0 0 0 0 0 00 0 0 0 0 0 00 0 0 00 00 0 0 000000 00 X XXXX XX XXXXx X X 0000000 HASKELL li ^ COMPOUND ANIMAL If GROUP II TREATMENT DAYS POST-TREATMENT DAYS DIET/DO SE VALUE i n it i a l EIGHT LOW W EIG HT FIN A L WEIGHT SEX 0 I EU/SACKlEICED TABLE XII-C HISTQPATHOLpGY TO-DATE SUMMARY RAT PAGE - 6 JULY TO, 197 2 7 570 03 96 500 96 7 235 03 96 500 96 7631 06 97 75 86 7 5 9 9 06 06 97 97 7625 06 97 ,7627 06 97 7628 06 97 7606 06 97 7608 06 97 7626 06 97 7593 06 97 7577 05 97 500 500 500 500 500 500 500 500 500 500 7 5 0 5 6 61 63 68 7 0 7 2 7 5 7 7 7 8 82 6 3 7565 05 97 750 63 7551 05 97 750 63 7560 05 97 750 66 632 Mc4 562 M S 265 F S 262 F S 259 F S 268 F S 301 F S 29 3 F S 281 F S 333 F S 366 F S 309 F S 507 M S 569 M S 552 M S 525 M S EYE FORMAL fV .P H U lU G IC S T R U C T U R ES EX'IR.-i 1 l'AL l A C P I MAI. Gl.ANU NUk MAL MOR PUIILUGIC STRUCTURE5 IDO. IN NUIMAL M U R P I l i I U l G I C S T R U C T U R E S M EDULLA IVIL'TNGATA NORMAL MOKPHOt llG I C STRUCT', j;-. 1 S S C I A T I C TIVRVf NORMAL M O RPHO LO GIC ST PliCTl.)-' [ S -SKIN MI.NMAl MNRI'HUI.UGIC S T R U C T U R E S S K I 11 UTG AN MI ' . S I M ' ; MAMMARY MIAMI) NORMAl MO:< P i l l l L I 101C ST KUC I U.- i. :> MAMMARY Gl AND ORGAN M I S S I N G I U N ] , H A P I' W NI.II'. MAI . M U H P M I I I O G I C S T R U C T fir' I- S S!'liTl:I N NORMAL MIJRPHUl I N . I I . S T R U C T U R E L Y ^ H NiiMT NORMAL MUKPHUUTGI C STRUC TU ", I .y W | | : n i l ' 'MRGAN M I S S I N G S K S . i. I AL M if. r. L t. NORMAL MO.'U' MUL UG IC ST S i c 1 UR!' S T K .& .H IA NORMAL M im i' H O L U G IC STP.UCTUP ES LUISSJ NORMAL MORPHOLOGIC S T R U C T U R E S l U I NTT' i *>T I T I AL PNEUMONIA I l f W t l HP MAI. MORPHOLOGIC S T R U C T U R E S AOSo.J* A NORMAL MOR PHilL OG I C S T R U C T U R E S S A lg V A R Y GLAND NORMAL MORPHOLOGIC S Tr'.U S T U 'U -S L I - S r NORMAL MORPHOLOGIC S T R U C T U R E S l ;\c s r g r a n u lo m a L I Y tIR CLOUDY SW ILLIN G L II^ R HYALINE DROPLET DEGENERATION 5 LI\O R CYTUPLASMIC LIPID DROPLET ALT 00 00 0 00 000 000 00 0 TABLE XII-C (Continued) HASKELL !i COMPOUND ' H I S T O P A T H O L O G Y T O - D A T E SUMMARY - RAT PAGE - 4-A JU LY 10, 1972 ANIM4L H LIVi-'R HYPERTROPHY PANCREAS OLOCIC STRUCTURES NORMAL MORPH ESOPHAGUS NORMAL MRPHUL0G1C STRU CT U R t" S STOMACH NORMAL HORPHu L O G IC s t r u c t u r PS DUODENUM NIJKMAL MORPHOLOGIC ST RU CT U RFS COLON NORMAL MORPHOLOGIC S T R U C T U R ES CECUM NORMAL MORPHOLOGIC S T R U C T U R E S 7576 7538 7631 7586 7599 7625 7627 7628 7604 7608 7626 7593 7577 7545 7551 7540 K I D N E Y NORMAL MORPHOLOGIC S T R U C T U R E S ! U R IN A R Y BLA D D ER NORMAL MOKPHULUGIC STRUCTURES P R O S T A T E AM . S E M IN A L V E S I C L E NORMAL MORPHOLOGIC STRUC IURI-.S T E S T I S NORMAL MORPhULOGIC ST R U CTU R E S ET UP RI DF SI D Y M I S NORMAL MORPHOLUGIC S T kU C UTfRUS CTURES NORMAL M U KP H U LO G iC STRU r A H .PIA N T U B E NORMAL MORPHOLOGIC S TRUCTURES OVARY NORMAL MORPHOLOGIC S T R U C T U R E S P H U I T ARY GLAND NORMAL MORPHOLOGIC S I r.ucruRE S AD RENAL GLAND NO.(MAL MORPH OLOGIC ST NOCTURES T H Y R O ID GLAND NORMAL M ORPH OLOGIC ST l-.UCTURCS T H YR O ID GLAND ORGAN M IS S IN G P A R A T H Y R O ID GLAND NORMAL MORPHLOGI C STRUCTURES PA R A TH Y R O ID GLAND ORGAN M IS S IN G THYMUS NORMAL MORPHOLUGIC ST R U C T U R E S Company Sanitized. Does not contain TSCA TABLE XIX-D ANIMAL H GROUP ii TREATMENT DAYS POST-TREATMENT UAYS im -T/DOSE VALUE I N I T I A I WEIGHT LOW WEIGHT G INAL Wr. 1GHT SEX O ITU/SACRIEICLD PAGE - 5 JULY 10, 1972 7552 05 97 7oo 68 7579 05 97 750 68 7567 05 97 750 70 7554 05 97 750 73 7562 05 97 750 76 7556 05 97 75o 78 7583 06 98 750 60 7630 06 98 750 62 76L5 06 98 750 63 7620 06 98 750 63 7622 06 98 750 67 482 M S 488 M S 540 M s 492 M s 530 M S 575 M s 345 F s 304 303 323 FFF SSs 291 F S 7616 06 9e 750 71 271 F S 7601 06 98 750 74 316 F s 7635 06 93 750 77 272 F S 7607 06 98 750 It 313 F S 7597 06 98 750 82 31.2 l S Company Sanitized. L-Y NORMAL MURPHOI.OG t C S T R U C T U R E S I X U x i W T A L L A C R IM A L GLAND NORMAL MUR CHCLUGIC STRUCTURES B R A I N NORMAL MOIL PHOLOG I C S T R U C T U R E S MEDULLA OBLONGATA NORMAL MQRPHOLO'Gl C STRUCTURES S C I A T I C NERVI- NORMAL MORPHOLUGIC ST KUCTUHES S K I N NORMAL M'TKP HIJI. Ur>I C S T R U C T U R E S SKIN URBAN M ISSIN G MAM/. ARY GLANU NORMAL M ORPHOLOGIC ST RCCTUnt'S MAMMARY GLAND ORGAN M I S S I N G BONE MARROW NORMAL MORPH OLOGIC STRU C I UR E S S P L E E N NUP.MAL MORPHOLOGIC S I R U C T U R C S LYMPH NUUL NORMAL MORPHOLOGIC ST K U C TUKC-S LYMPH iv.IOU ORGAN M I S S I N G S K E L E T A L MUSCLE NORMAL MORPHOLOGIC STRUCTimi S TRACHEA NORMAL MORPHOLOGIC S T K U C T U R ES ' LUNG NURMAL MORPHOLOGIC ST R U C T U R ES o UJMC I N T E R S T I T I A L PNEUMONIA HEAP. T NORMAL ROK PH.ILG I C S T R U C T U R E S w AORTA NORMAL MORPHOLOGIC S T R U C T U R ES 3O S A L IV A R Y GLAND NORMAL MORPHOLOGIC S O TRUCTURES 5. L I V E R NORMAL MORPH OLOGIC S T R U C T U R E S L I V E R GRANULOMA ^ L IV E R CLOUDY SH ELLIN G JJJ L I V E R H Y A L I N E O R U P L E T D E G E N E R A T IO N o > O LIV ER 03 CYTOPLASMIC LIP ID DROPLET ALT 00 0 0 0 0 0 0 0 0 0 0 00 00 TABLE XII-D (Continued) Company Sanitized. Does not contain TSCA CBt H A SKELL it COMPOUND PAGE - 5--A JULY 10, 1972 ANIMAL " l iv e r hypertrophy PANCREAS ULCGIC STRUCTURES NORMAL MORPH ESOPHAGUS NORMAL MORPHOLOGIC STRU CT URLS STOMACH NORMAL MORPHOLOGIC STRU CTUR ES DUODENUM NORMAL M OR PH ULUGIC S T R U C T U RES COLON NORMAL M ORPHOLOGIC S T R U C T U R E S CECUM NURMAL MORPHOLOGIC S T R U C T U R ES K I N E Y NURMAL MUI'.PH'TL OGI C S T R U C T U R E S U R IN A R Y B LA D D ER NURMAL MOPPMULOi.l STKIJC TURF S P R O S T A T E AN ij S E M IN A L V E S I C L E NORMAL MOP. POOL OG I (. S T R U C T U R E S T E S T I S NORMAL MORPHOLOGIC S T R U C T U R E S E P I D I D Y M I S NURMAL MORPHOLOGIC STKUC TURPS UTERUSCTURES NORMAL MORPHOLOGIC STKU F A L L O P I A N 1'UI` f NORMAL M O R PH U LU G IC S TRUCTURFS OVARY NORMAL MORPHULUGIC S T R U C T U R ES P I T U I T A R Y GLANO NORMAL MORPHOLOGIC STRUCTURES ADRENAL GLANU NORMAL MORPHOLOGIC ST KUCTUR t:S T H Y R O ID GLAND NORMAL MORPHOLOGIC ST R U C TUR I S T H Y R O ID GLAND ORGAN M IS S IN G P A R A T H Y R O ID GLANO NORMAL MRPHLOGI C STRUCTURES PA RA TH Y R O ID GLAND ORGAN M IS S IN G THYMUS NORMAL MORPHOLOGIC S T R U C T U R E S 7552 7579 7567 7554 7562 7556 7533 7630 7615 7620 7622 7616 7601 7633 7607 7597 TABLE XIII BODY WEIGHTS OF INDIVIDUAL MALE DOGS FED Grpup I (Control) Dog ________ ____________ No. 0____ 1_____ 2 ,3 1150 1154 1158 1162 8.1 11.2 11.4 9.5 8.2 10.9 11.4 9.2 8.6 11.0 11.4 9.2 8.1 11.1 11.6 9.4 Weekly Weight in Kilograms _____5 6 7_____ 8 9 7.9 11.0 11.1 9.3 8.0 11.0 11.5 9.5 8.1 10.8 11.5 9.6 8.2 11.2 11.8 9.7 8.2 11.1 11.8 9.5 8.3 11.3 12.0 9.6 10 8.2 11.4 11.8 9.7 11 8.4 11.2 11.7 9.6 12 8.4 11.4 12.0 9.7 13 8.4 11.5 11.9 9.9 Avg. 8.2 11.2 11.6 9.5 II (500 1153 1155 1159 1165 10.8 9.3 14.0 9.0 11.3 9.2 14.9 9.5 11.1 9.4 14.0 9.5 11.4 9.5 14.3 9.8 11.1 9.4 14.2 9.5 11.2 9.4 14.2 9.6 11.3 9.5 14.3 9.4 11.5 9.6 14. 6 9.8 11.4 9.8 14.4 9.6 11.6 9.8 14.8 9.8 11.2 9.8 14.8 9.9 11.5 10.0 14. 9 9.8 11.7 10.0 14.7 9.7 11.6 9.8 15.0 9.9 11.3 9.6 14.5 9.6 I8 o 1152 1156 1160 1164 11.2 8. 3 9.1 7.8 11.0 8.2 9.1 7.5 11.1 8.3 9.0 7.6 11.3 8.4 9.1 8.0 11.4 8.1 9.1 7.8 11.2 8.1 9.3 7.9 11.3 8.2 9.3 7.8 11.7 8.3 9.5 8.2 11.3 8.4 9.4 8.1 11.4 8.2 9.4 8.0 11.7 8.6 9.4 8.0 11.5 8.3 9.4 8.0 11.6 8.4 9.4 7.9 11.8 8.3 9.4 8.0 11.4 8.3 9.3 7.9 1 IV (2,500 ppm 1151 1157 1161 1163 10.8 10.5 9.0 9.8 10. 6 10.8 8.7 10.0 10.6 10.7 8.8 10.1 10.7 11.0 8.8 10.2 10.4 10.8 8.8 10.0 10.8 10.6 8.8 10.2 10.8 10.8 8.5 10.3 10.8 10.9 8.9 10.5 10.8 10.8 8.8 10.5 11.0 11.2 8.9 10.4 11.0 11.1 8.8 10.6 10.9 11.0 8.8 10.8 10.7 11.1 9.0 10.5 10.5 10.9 8.9 10.5 10.7 10.9 8.8 10.3 Sanitize3-Does 3 C> oo 3 , 3 4 OJ o > o TABLE XIV . BODY WEIGHTS OF INDIVIDUAL FEMALE DOGS Group I (Control) Dog No. 1137 1141 1145 1147 1135 1140 1143 1149 1136 1139 1144 1148 1134 1138 1142 1146 -- ------------------- ------ ------ ------ -- 10.5 10.6 10.7 10.9 11. 8 12.0 12.0 12.0 8.0 7.8 8.0 8.3 8.9 8.7 8.6 8.8 4 10.7 12.2 8.2 8.6 Weekly Weight in Kilograms_____ _______ -5 6 7 9 10 11 10.8 12.0 8.2 9.0 10.9 11.7 8.4 9.1 11.0 11.6 8.5 9.2 11.0 11.5 8.5 9.2 10.8 11.5 8.4 9.4 10.9 11.4 8.8 9.3 11.0 11.5 9.0 9.4 8.2 8.1 8.1 8.3 8.4 8.5 8.7 8.5 8.5 8.5 8.2 8.3 1u 7.0 7.0 7.2 7.0 7.1 7.2 7.3 7.3 7.2 7.3 7.3 8.1 8.8 8.0 8.2 8.0 8.4 8.8 8.6 8.6 8.4 8.5 8.6 6.3 6.5 6.6 6.8 6.8 6.8 6.8 6.9 6.9 7.0 7.1 7.0 13.0 8.0 9.2 9.6 13.3 7.8 8.5 9.8 13.2 7.8 8.3 9.4 13.3 7.9 8.8 9.6 13.4 7.9 8. 6 9.4 13.7 8.0 8.8 9.8 13.8 8.0 8.7 9.8 14.1 8.4 8.9 10.0 14.4 8.5 8.8 10.0 14. 9 8.4 8.8 10.2 15.2 8.4 8.8 10.2 15.3 8.7 8.9 10.2 6.0 6.1 6. 1 6.2 6.1 6.2 6.4 6.4 6.5 6.6 6.6 6.7 8.8 8.4 8.9 9.4 9.3 9.5 10.0 9.8 10.2 9.8 9.8 9.5 9.6 9.2 9.0 9.2 8.8 8.4 8.4 8.4 8.5 8.6 8.5 8.4 7.0 7.0 7.1 7.0 6.9 6.8 6.9 6.8 7.2 7.0 6.8 6.6 12 11.0 11.4 9.2 9.5 8.2 7.3 8.8 6.9 15.3 8.7 9.0 10.7 6.7 9.4 8.4 6.4 13 Av p 7 10.9 11.5 9.1 9.4 10 8 11 7 84 9.1 8.4 8 4 7.2 7.2 8.6 8.4 7.0 6. 8 15.1 8.5 9.0 10.6 14.1 8.2 8.8 10.0 6.7 6.4 9.2 9.4 8.3 8.7 6.5 6.8 Company Sanitized. Does not contain 1 tan a os TABLE XV DIET CONSUMPTION OF INDIVIDUAL MALE DOGS FED Group I (Control) Dog -- AveraSe Dally Diet Consumption in Grams During Week No. 1 2 3 4 5 6 7 8" * 9 10 11 12 13 Avg. 1150 1154 1158 1162 290 328 410 318 350 365 400 316 289 340 390 361 295 363 370 336 313 310 432 345 326 381 335 358 415 336 350 393 351 317 325 384 258 291 374 417 318 360 324 375 321 346 391 394 339 331 364 4,67 382 321 348 402 332 1153 345 389 380 427 439 384 1155 326 403 339 392 417 387 1159 429 555 500 489 541 517 1165 330 352 339 346 338 320 415 381 408 373 419 383 385 394 414 404 348 421 394 416 345 385 567 502 525 544 569 461 526 517 386 327 381 361 375 352 418 356 1152 1156 1160 1164 320 241 314 308 441 279 356 333 435 269 375 306 429 254 381 350 329 267 349 286 382 263 326 259 414 270 327 315 429 301 359 328 390 256 293 262 381 322 322 293 333 343 389 281 412 356 352 221 408 262 369 263 392 283 347 293 is N 05 1151 320 416 396 421 438 370 1157 379 417 418 404 432 387 1161 260 306 370 347 305 314 1163 296 333 352 318 370 341 409 429 430 380 448 371 330 397 426 426 361 382 420 382 421 404 371 384 314 304 355 355 362 334 330 356 293 314 323 300 341 328 N<0 a oaitsi O3 containTSCACBli v_* TABLE XVI DIET CONSUMPTION OF INDIVIDUAL FEMALE DOGS FED Average Daily Diet Consumption in Grams During Week (Control) Avg. 1137 1141 1145 1147 382 428 263 200 396 373 287 198 406 372 303 280 364 453 284 321 338 287 281 334 378 386 286 280 331 290 262 327 391 395 259 321 312 356 226 359 316 401 311 295 338 470 312 318 358 452 285 306 374 500 274 330 364 389 285 296 II (5.00 1135 1140 1143 1149 251 265 258 349 323 267 297 362 329 283 375 369 332 277 380 362 320 254 392 412 329 319 273 329 386 343 359 369 35,6 355 301 232 338 362 366 373 305 231 326 337 244 200 247 367 202 261 349 381 331 285 338 409 307 266 338 370 III (750 aa> IV (2,500 fCacD'. a o 1136 1139 1144 1148 427 304 197 346 409 317 290 372 368 317 304 361 426 357 305 364 388 429 450 309 283 '319 298 . 313 291 382 353 335 492 331 271 318 538 293 259 360 449 257 296 244 455 332 308 272 403 310 317 402 420 240 307 380 435 305 289 345 1134 1138 1142 1146 279 300 275 244 270 448 277 267 280 422 309 261 284 414 277 252 320 350 183 284 293 311 399 377 261 344 256 255 261 370 328 282 253 398 342 217 251 352 256 198 276 233 352 276 260 254 421 104 280 319 429 248 278 357 320 242 not contain TS C A CBJ TABLE XVII average doses of individual male dogs fed Group I Control Dog No. 1150 1154 1158 1162 1 Average Dally Dose in Milligrams/Kilograms during Week 2 -- -----^____5_____ _____ 7 8 9 10 11 12 13 Avg. II 500 1153 1155 1159 1165 45 51 42 51 50 62 55 53 17 18 18 18 19 20 21 22 17 19 18 18 17 21 18 17 18 22 20 20 17 21 17 17 18 16 18 22 18 18 20 18 18 20 19 19 16 21 16 18 17 17 18 21 22 26 23 24 III 750 ppm 1152 1156 1160 1164 62 63 74 87 86 73 85 94 29 24 31 29 28 22 23 25 31 28 33 28 26 24 26 25 27 25 26 30 28 27 29 30 26 25 23 29 23 26 25 28 22 31 31 26 27 32 28 21 26 23 29 25 33 32 36 37 O IV 2,500 1151 1157 1161 1163 192 252 230 253 190 223 192 214 94 97 105 86 99 103 93 101 99 87 79 92 86 95 99 90 98 99 91 106 109 84 79 85 99 86 102 86 78 113 82 85 96 87 96 116 89 86 101 100 101 114 70 75 75 71 81 99 crnpany Sanitized. Does not contain TSCA CBf % TABLE XVIII AVERAGE DOSES BY INDIVIDUAL FEMALE DOGS FED Group I Control Dog No. 1137 1141 1145 1147 -- 7y---- Average Daily Dose In Milligrams/Kilogram during Week 10 11 12 13 Avg. II 500 1135 1140 1143 1149 44 54 44 78 57 54 50 78 20 20 23 28 20 20 24 27 19 18 24 30 19 19 22 26 19 23 20 27 21 21 20 26 21 18 16 16 21 19 27 24 15 14 14 26 12 18 20 27 20 20 19 29 24 24 25 35 III mm 1136 1139 1144 1148 69 82 50 76 66 87 74 83 21 30 26 28 24 34 26 29 21 23 29 26 26 27 30 . 27 24 29 25 25 26 30 23 24 28 22 26 23 22 25 27 18 22 29 26 20 20 27 26 29 21 21 26 27 30 36 31 34 O O T3033 3 IV 2,500 ppm 1134 1138 1142 300 224 188 284 334 195 115 115 85 114 110 77 129 93 53 116 108 78 121 95 102 102 92 98 I 1146 224 245 93 90 104 94 94 101 96 95 105 97 105 137 99 90 61 67 86 121 99 104 105 125 128 110 76 72 103 40 97 110 Sanitized. Does not contain TSCACBt Ccmpsny S an d ed . Does not contain TSCACBS TABLE XIX SUMMARY OF HEMATOLOGIC MEASUREMENTS ON DOGS FEDI ppm in Diet Erythrocytes x 106/mm3 0 500 750 2,500 Hemoglobin gm/100 ml 0 500 750 2,500 Hematocrit la 0 500 750 Leucocytes x 103/mm3 2,500 0 500 750 2,500 0 Segmented Neutrophils % 500 750 2,500 0 Non-segmented Neutrophils 500 % 750 2,500 0 Metamyelocytes 500 7,, 750 2,500 Pre-test 6.62 6.05 6.10 6.24 16.7 15.6 15.9 16.0 44 41 42 42 11.8 12.7 11.1 11.4 66 64 65 68 0.8 0 0.4 0.5 0 0 0 0 MALES Month: on Test 123 7.51 7.33 7.05 6.87 6.70 6.54 6.55 6.65 6.61 6.59 6.36 6.09 J.8.0 18.0 18.2 16.7 16.7 16.3 16.3 16.6 16.9 15.7 15.5 15. 3 49 48 48 46 46 43 44 44 46 42 41 41 11.6 14.3 11.4 13.0 12.9 13.7 12.5 11.1 13.1 13.2 13.5 15.4 67 63 63 62 68 67 70 66 69 64 68 71 0.3 0.3 0.5 0.8 0.5 0.5 0.3 0.5 0 1.5 0 1.5 000 000 000 000 FOR THREE MONTHS Pre-test 6.31 6.31 6.47 6.65 16.8 15.9 16.7 17.2 43 43 44 45 10.5 12.0 12.5 11.1 65 70 67 62 0.8 0.9 1.4 0.4 0 0 0 0 FEMALES Months on Test 12 3 6.61 6.93 6.66 5.86 6.77 6.31 6.46 7.20 6.95 5.85 5.93 6.09 16.3 17.8 17.5 15.7 16.9 16.0 17.3 17.9 17.5 14.9 44 15.0 47 15.5 46 43 44 42 47 47 46 40 38 41 12.2 12.3 9.5 10.6 12.9 10.3 13.2 14.8 11.3 11.1 12.5 9.4 72 72 64 66 68 67 69 66 61 62 70 63 0 0.5 0.5 0.5 0.5 1.0 0.8 1.3 1.0 0.8 1.0 0.8 000 000 00 0 000 Ser^any Sanitized, Dses noi contain TSCA CB TABLE XIX (Continued) SUMMARY OF HEMATOLOGIC MEASUREMENTS ON DOGS F E D Lymphocytes % Eosinophils 7= Monocytes lo Basophils % Atypical Cells % Nucleated R B C 's per 100 WBC's ppm in Diet 0 500 750 2,500 0 500 750 2,500 0 500 750 2,500 0 500 750 2,500 0 500 750 2,500 0 500 750 2,500 Pre-test 27 31 30 29 5 4 3 3 1.3 1.4 1.6 . 0.1 0 0 0 0 0 0 0 0 0 0 0 0 MALES Months on Test 123 25 28 26 32 26 27 27 26 24 26 26 21 7 9 11 456 276 85 6 1.5 1.0 0.8 1.8 0.8 0.5 1.3 0.5 0.8 0.3 1.5 0.5 000 000 000 000 000 000 000 000 000 000 000 00o FOR THREE MONTHS Pre-test 25 25 27 28 7 2 3 8 1.9 1.9 1.0 1.4 0 0 0 0 0 0 0 0 0 0 0 o FEMALES Months on Test 12 3 25 25 27 30 27 25 25 27 30 29 26 30 3 3 .8 34 7 65 7 73 6 0.3 0.5 0 0.8 0.3 0.5 0.5 0.5 0.8 1.5 0.8 1.3 000 000 000 00 0 000 000 000 000 000 00 0 000 000 TABLE XX SUMMARY OF URINALYSIS D A T A ,ON DOGS FEDI FOR THREE MONTHS Pre-Test 159 169 153 203 1648 1366 1635 1527 177 130 151 119 0 1 0 0 0 0 0 0 0 0 0 0 2 0 3 0 FEMALES Months on Test 12 3 126 134 150 140 149 150 150 97 140 209 1900 178 1794 151 1684 1639 1725 1861 1627 1456 175 1348 1594 184 1807 1859 170 113 154 160 112 126 194 106 125 170 000 00 0 020 000 000 000 00 0 00 0 000 000 000 000 34 3 12 4 34 4 022 Average of the measurement per dog for volume, osmolality, creatinine; number positive or abnormal in eight specimens for blood, sugar, acetone, bilirubin, protein. Company Sanitized. Doei Pempany Sanitized, TABLE XX (Continued) SUMMARY OF URINALYSIS DATA ON DOGS f e : Protein Number Abnormal Urobilinogen Erythrocytes per hpf Leucocytes per hpf Epithelial Cells per hpf Casts per lpf ppm in Diet 0 500 750 2,500 0 500 750 2,500 0 500 750 2,500 0 500 750 2, 500 0 500 750 2,500 0 500 750 2,500 Pre-Test* 0 0 0 0 0.9 0.4 0.8 0.7 0 0 0 0 0-4 0-2 0-6 0-6 0-1 0-1 0 0 0 0 0 0 MALES Months on Test 123 0 0 0 0 1.0 0.6 0.8 0.6 0 0 0 0 0-3 0-1 0-2 0-10 0-3 0-1 0-1 0-1 0 0 0 0 0 0.8 0.6 0.6 0.8 0 0 0 0 0-1 0-1 0-4 0-5 o 0 0 0 o 0 0 0 0 0.8 0.8 1.0 08 0 0 0 o 0-1 0-10 0-5 0-3 0-1 0-1 0-2 0-2 o 000 000 000 OR THREE MONTHS Pre-Test 0 0 0 0 0.9 0.3 0.8 0.4 0 0 0 0 0-2 0-2 0-2 0-2 0-2 0-2 0-1 0-3 0 0 0 0 FEMAT.ES Months on Test 12 3 000 000 000 000 1.0 0.8 1.0 1.0 1.0 0.8 1.0 0.6 1.0 0.6 1.0 1.0 000 000 O 0-6 0 000 0-8 0-1 0-2 0-2 0-1 0-1 0-1 0-2 0-5 0-1 0-1 0 0-3 0-1 0-5 0-5 0-1 0-4 0-1 0-2 0-4 0-1 0-2 0-1 000 000 00 0 000 Company Sanitized. Does TABLE XXI SUMMARY OF BIOCHEMICAL MEASUREMENTS ON DOGS FE! Glucose mg % Urea Nitrogen mg % Cholesterol mg % Alkaline Phosphatase Bessey Units Transaminase Reitman-Frankel Units Total Protein g/100 ml PPm in Diet 0 500 750 2,500 0 500 750 2,500 0 500 750 2,500 0 500 750 2,500 0 500 750 2,500 0 500 750 2,500 Pre-test 123 128 111 114 14 16 14 17 137 146 161 148 4.2 6.4 4.4 4.4 8 7 8 7 6.3 6.2 6.1 6.0 MALES Months on Test 12 3 137 125 116 140 126 112 111 116 109 101 117 107 14 15 14 22 19 20 21 17 16 23 21 19 133 150 138 140 174 163 184 206 189 181 206 188 3.2 6.9 6.6 10.7 17 14 15 11 3.3 8.1 6.9 11.3 15 15 18 15 3.0 8.2 5.3 11.7 13 11 17 13 6.1 6.2 6.4 6.0 6.2 6.2 5.9 6.1 6.2 6.2 6.3 6.3 3 Oc-- oo 3 a <n O O m 'OR THREE MONTHS Pre-test 112 119 107 107 14 17 18 16 158 156 157 159 4.3 6.4 4.4 5.2 6 6 5 5 6.2 6.3 6.1 6.3 FEMALES Months on Test 12 3 108 123 106 112 108 106 95 113 105 96 114 112 21 22 27 21 25 25 23 19 21 21 21 18 164 168 195 180 205 230 209 258 293 277 314 256 3.9 8.4 6.2 10.5 3.6 7.4 7.2 12.3 5.2 6.1 7.3 11.6 9 14 12 12 10 10 10 11 9 13 18 15 6.0 6.0 6.3 5.8 6.2 6.5 6.1 6.5 6.8 6.4 6. 6 6.5 TABLE XXI (Continued) SUMMARY OF BIOCHEMICAL MEASUREMENTS ON DOGS FED Albumin/Globulin Albumin g/100 ml Creatinine mg % Bilirubin mg % ppm in Diet 0 500 750 2,500 0 500 750 2,500 0 500 750 2,500 0 500 750 2,500 Pre-test 1.12 1.15 1.11 1.07 3.3 3.3 3.2 3.1 0.6 0.7 0.6 0.7 0.2 0.1 0.2 0.2 MALES Months on Test 12 1.27 .1.16 1.25 1.05 3.4 3.2 3.3 3.1 0.6 0.6 0.5 0.6 0.4 0.4 0.3 0.3 0.97 1.15 1.18 0.98 3.0 3.3 3.3 3.1 0.8 0.8 0.7 0.7 0.3 0.2 0.2 0.2 3 1.00 1.03 1.02 1.13 3.2 3.1 3.1 3.3 0.8 0.9 0.9 0.8 0.2 0.2 0.2 0.2 FOR THREE MONTHS Pre-test 1.11 1. 14 1.19 1.16 3.2 3.3 3.3 3.3 0.7 0.7 0.7 0.7 0.1 0.1 0.1 0.2 FEMALES Months on Test 12 1.29 1.05 1.16 0.98 3.3 3.0 3.2 3.2 0.7 0.6 0.7 0.6 0.2 0.3 0.4 0.3 1.09 0.97 1.07 0. 93 3.2 3.1 3.4 3.1 0.9 0.7 0.8 0.8 0.2 0.4 0.3 0.3 3 1.29 1.14 0.91 1.12 3.5 3.4 3.2 3.4 1.1 0.8 0.7 0.9 0.2 0.6 0.5 0.3 maany Sanitized. Does not c o3(# SB, 5" ~i tn o > o 131 ORGAN WEIGHTS IN GRAMS OF INDIVIDUAL MALE Brain Heart Lungs Liver Group I Control II 500 III 750 ppm O IV o 2,500 i 3 *f1 *3< cn Body W.t, Dog No. (kg) 1158 1154 1162 1150 12.0 11.5 11.0 8.0 90 106 127 397 73 102 175 332 74 111 132 301 77 76 95 270 1153 1165 1159 1155 11.5 73 108 174 408 9.9 '' 71 90 144 400 14.9 85 143 191 593 10.0 75 74 153 370 1164 1160 1152 1156 8.2 9.8 11.8 8.6 69 68 120 320 82 95 121 357 91 88 157 454 85 60 121 309 1163 1161 I 1151 1157 10.3 9.0 11.0 11.0 84 84 113 486 81 80 113 493 75 87 149 541 90 101 197 516 NO p. C3 o.'ft not contain TSCA o 03 TABLE XXII DOGS SACRIFICED FTER THREE MONTHS 1 FEEDING OF |Spleen |Kidney |Testis Prostatf (Bladder (stomach Thymus Adrenals Pituitar Thyroid W >*> <u u V 0 cd Pu 30 40 64 25 11.8 5.2 110 27 32 70 19 7.0 6.9 95 25 29 69 24 5.0 6.3 92 24 25 47 14 4.6 4.6 86 9.5 1.3 0.068 0.85 8.3 1.7 0.059 0.92 5.8 1.2 0.070 0.76 9.2 1 . 0 0.056 0.74 36 25 73 24 26 29 59 21 22 32 77 32 20 25 70 14 5.3 5.6 93 36.3 1.5 0.077 0.45 4.9 5.0 93 13.7 1.2 0.068 0.25 8.0 8.8 128 13. 7 1.8 0.084 1.02 8.9 4.9 86 4.3 1.1 0.066 0.91 15 32 57 17 6.4 6.2 74 8.8 0.8 0.059 0.25 24 29 55 17 5.9 4.8 80 13.2 1.4 0.044 0.65 29 26 59 17 11.2 4.9 108 9.6 1.6 0.057 0.96 23 18 46 17 7.3 3.2 78 8.7 0.9 0.064 0.57 24 25 68 25 20 18 62 19 22 22 60 20 6.2 5.6 99 11.0 1.5 0.084 1.21 3.4 4.4 87 9.8 1.1 0.064 0.65 3.3 5.6 108 16.6 1 . 0 0.050 1.10 TABLE XXIII .QRGAiyjElGHTS IN GRAMS OF INDIVIDUAL FEMALE POOS SACRIFICED AFTER THREE MONTHS' FEEDTWC Of J HeartJ LungsJ Liver Ispleen Pancreas Kidney Stomachj 'Adrenal Pituitary Thyroid jroup Control Body Wt. C H 03 to 3 __ g) PQ 1H 107 107 65 94 172 161 140 152 349 372 347 308 25 23 19 28 24 20 26 31 57 66 46 62 103 111 83 92 6.2 14.5 7.6 12.3 1.1 1.8 1.7 2.0 0.072 0.058 0.059 0.078 1.19 1.03 0.81 0.65 'oC3 2 *co eI-Sj- CS3S =?. <mO0L" a o qS/3 3O c o3 9), 5' w o > o *33 IV 2,500 ppm 6. 8 9. 2 8. 6 6. 8 72 82 76 73 55 90 342 11 21 39 80 139 520 17 24 54 79 134 430 21 24 53 67 30 351 12 22 42 76 6.3 1.2 0.061 0.57 80 11.7 1. 8 0.049 0.37 76 9.0 1.2 0.056 0.74 70 10.2 1.7 0.035 0 . 2 2 TABLE. XXIV a v e r a g e l i v e r w e i g h t s a n d l i v e r /b o d y w e i g h t r a t i o s o f d o g s f e d Group I II III IV ppm Zonyl RP 0 500 750 2,500 Final Body Weight (kg) 10.4 11.6 9.6 10.3 MALES Liver Weight Liver Weight ,, (g) Body Weight * iUU 325 3.15 442 3.82 360 3.75 509 4.95 Final Body Weight (kg) 10.5 8.0 11.0 7.8 FEMA]L.ES Liver Weight Liver Weight (g) X 100 344 3.29 346 4.28 413 3.83 411 5.22 Company Sanitized. Does not contain o33 ! TABLE XXV Histopathology - Group I (Control) - H-72J-7 RESPIRATORY AND CARDIOVASCULAR DIGESTIVE GENITOURINARY (V > .-P w *H B C H fHt O rH H --1 w P*-t <13 . -p 3 pi pC w <D o P to fHt <D m 0> S Company Sanitized. Does not contain Code: A = Focal pleural fibrosis. B = Focal germinal cell atrophy or hypoplasia C = Focal suppurativepneumonitis. - = No abnormalities detected. + = Slight degree of lesion. CO o > o CD -. TABLE XXV (Continued) Histopathology - Group I (Control) H-72^7 NERVOUS AND MDOCRDfli:_________ . .MUSCULOSKELETAL , SKIN AND APPENDAGES. HEMIC AND EMPHATIC Pituitary Thyroid Parathyroid Adrenal Skeletal Mus cle Sciatic Nerve Brain Spinal Cord Mammary Gland Skin Bone Marrow Spleen Lymph Node Company Sanitized. Does Dog No. Days On Test Sex 1150 10b d --0 - - - _ p&i W I _ 1154 98 d - - 0 - - - - - - 0 - - _ _ _ 1158 98 d - - 0 - - - - - - 0 - - - _ _ II62 98 d - - - - - - - - - 0 0 - - - - 1137 99 $ - - - - - - - - - 0 - - - - iia 99 ? - - 0 - - - - - - 0 - - - - - 1 1 A5 1 1 A7 99 --- - - - - - - - - - - - - 99 ? _ _ __ 3 ^ Code: - = No abnormalities detected, o3 ST 0 s No tissue available on slide. 5* 05 O > a TO TABLE XXVI Histopathology - Group IV - H-72**7 RESPIRATORY AND CARDIOVASCULAR DIGESTIVE (# GENITOURINARY Lung Upper Trachea Heart Aorta Stomach 1 Duodenum Cecum Colon Salivary Gland Liver Esophagus Fallopian Tube Uterus/ Prostate Dog No. Sex 02 C0) w U O S ra cd Ph ai |> HO 1157 1151 II6 1 II63 113*4- 111*2 11**6 II38 U C+ - - - - _ D+ B+ d" - - - - - - - - _ D+ _ B+ ---- - - _ _ D+ _ <S C+ - - - - - - - - - D+ _ 2 -- - - __ _ D+ ? C++ - - - - - - - _ D+ _ $ ----- -- - D+ _ ? ------ - - - D+ Code: C = Focal suppurative pneumonitis. B = Focal germinal cell atrophy or hypoplasia. D s Hepatocyte hypertrophy and degeneration. - = No ahnormalities detected. + = Slight degree of lesion. ++ = Moderate degree of lesion. Compare Sanitized. Does nocontain TSCA G?\ TABLE XXVI(Continued^ Histopathology - Group IV - H-721+7 ENDOCRINE NERVOUS AND MUSCULOSKELETAL Dog No. Sex u cd -P H -P OH rH H O r *CP UC Ph C dCD h C 1157 1151 l l 6l 116 3 Itse l l j k <3 llk 2 37Ns?. II4-6 1138 5 Ootfl ---3o Code: o o3 3 0 O > o U U cT cT $ ? S '$ E = Subcapsular eosinophil infiltration No abnormalities detected. 0 = No tissue available on slide. + - Slight degree of lesion. t oo rCH H CPOi SKIN AND APPENDAGES g "d TO r--1 sO 0 0 0 0 0 0 Skelet; Muscle Sciatii Nerve Brain Eye Skin Spleen HEMIC AND LYMPHATIC li cd g 'o(dL> W& O) o 1 & pq g S' E+ TABLE XXVII Histopathology - Group II - K-7 2k 7 Dog No. 1155 1159 1165 1153 11^9 llit-3 uko 1135 Sex Liver d" cf icf - ? ? $ 5. _ Code: - a No abnormalities detected. pfiifiy 'Sanitized. Does not contain TABLE XXVIII Histopathology - Group III - H-72V7 Dog No. Sex Liver 1156 c? - 1152 - ll60 n6k d1 d1 - 1136 UM U39 lli)-8 0- ?0?- Code: - a No abnormalities detected. @fnpany Sanitized. Does not contain T S C A CB< I APPENDIX I not contain TSCA CEB com pany Sanitized. Does UiiJLJNlUALi LABORATORY DATA ON INDIVIDUAL DOGS FED 'OR THREE MONTHS Dog No.: 1150 Sex: Male Group : Control____________________________________ Months on Test tiemacoiogy Control 12 Erythrocytes Hemoglobin Hematocrit Leucocytes DIFFERENTIAL Neutrophils Seg. Juv. Myel. Lymphocytes Eosinophils Monocytes Basophils Atypicals Nucleated RBC - 6.56 16.1 40 8.1 63 62 1 0 24 10 2 1 0 0 6.84 17.4 45 8.5 52 52 0 0 44 4 0 0 0 0 7.84 18.6 50 9.3 74 73 1 0 21 4 1 0 0 0 7.24 18.3 49 11.2 50 50 0 0 28 22 0 0 0 0 3 7.28 19.2 49 11.5 57 57 0 0 28 13 2 0 0 0 Biochemistry Glucose Urea Nitrogen Cholesterol Aik. Phos. GPT Total Protein A/G . Creatinine Bilirubin Albumin 118 22 110 2.9 6 6.4 1.10 0.6 0.3 3.7 119 17 130 3.0 5 6.3 1.28 0.8 0.4 3.5 120 17 120 2.6 16 6.2 1.44 - 0.5 0.4 3.7 132 19 130 2.5 16 6.2 1.02 0.8 0.5 3.1 134 13 120 2.3 14 6.1 0.97 0.7 0.2 3.0 Urinalysis Volume Appearance Osmolality .Occult Blood Sugar Acetone pH Urobilinogen Bilirubin Protein Creatinine MICROSCOPIC RBC 's WBC 's Epithelial Bacteria Cast Sperm 185 D,Y,C1,P 1950 N N N 6.8 1.0 P Tr 132 0 0-2 0 2+ 0 0 160 D,Y,C1,P 1373 N -N N 7.0 1.0 P Tr 108 0 1-4 0 2+ 0 1-5 155 A, Cl 2146 N Tr N 6.8 1.0 P 1+ 150 0 0-3 0 2+ 0 0 125 D, Y, Cl, P 2159 N N N 6.8 1.0 P 1+ 166 0 0-1 0 2+ 0 2-6 110 D,Y, Cl 2081 N N N 7.0 1.0 P 1+ 212 0 0 0-1 2+ 0 5-10 Com pany Sanitized. Does not contain T S C A C B I Dog No.: 1154 Sex: Male Group : Control Hematology Erythrocytes Hemoglobin Hematocrit Leucocytes DIFFERENTIAL Neutrophils Seg. Juv. Myel. Lymphocytes Eosinophils Monocytes Basophils Atypicals Nucleated RBC Biochemistry Glucose Urea Nitrogen Cholesterol Aik. Phos. GPT Total Protein A/G Creatinine Bilirubin Albumin Urinalysis Volume Appearance Osmolality Occult Blood Sugar Acetone pH Urobilinogen Bilirubin Protein Creatinine MICROSCOPIC RBC 's WBC 's Epithelial Bacteria Cast Sperm CLINICAL LABORATORY lTA o n i n d i v i d u a l dogs f e d - 'OR THREE MONTHS Control 7.28 18.7 51 15.0 74 71 3 0 22 4 0 0 0 0 7.24 18.1 48 11.5 70 70 0 0 24 6 0 0 0 0 150 13 145 4.7 12 6.4 1.04 0.5 0.1 3.3 124 10 175 4.9 5 6.6 1.07 0.8 0.1 3.4 245 Y.Cl.P 1357 N N N 7.0 1.0 P 1+ 130 0 0 0-1 2+ 0 0-1 275 Y, Cl, P 1194 N N N 7.2 0.1 P N 114 0 0 0 1+ 0 0 Months on Test 12 8.04 19.4 54 12.5 67 67 0 0 25 7 1 0 0 0 7.88 19.0 49 12.1 63 63 0 0 32 5 0 0 0 0 3 7.64 19.2 51 11.7 68 66 2 0 23 9 0 0 0 0 180 14 145 3.4 20 6.2 1.17 0.6 0.4 3.3 126 10 160 3.8 13 6.3 1.14 0.8 0.2 3.4 124 12 150 3.3 12 6.7 1.24 0.8 0.2 3.7 205 A, Cl 1740 N Tr N 7.0 1.0 P Tr 138 0 0 0-1 2+ 0 1-4 270 Yj Cl,P 1142 N *N N 7.2 0.1 N Tr 114 0 0 0 1+ 0 2-4 185 D,Y, Cl, P 1845 N N N 7.2 1.0 P Tr 180 0 0 0-1 1+ 0 0 Com pany Sanitized. Does not contain *? t Dog No.: 1158 Sex: Male Group :___ Control dematoiogy Erythrocytes Hemoglobin Hematocrit Leucocytes DIFFERENTIAL Neutrophils Seg. Juv. Myel. Lymphocytes Eosinophils Monocytes Basophils Atypicals Nucleated RBC Biochemistry Glucose Urea Nitrogen Cholesterol Alk. Phos. GPT Total Protein A/G Creatinine Bilirubin Albumin tinalysis Volume Appearance Osmolality Occult Blood Sugar Acetone pH Urobilinogen Bilirubin Protein Creatinine MICROSCOPIC RBC 's WBC's Epithelial Bacteria Cast Sperm CLINICAL LABORATORY JDATA ON INDIVIDUAL DOGS f e d I M M B f o r THREE MONTHS Control 6.56 16.1 42 9.9 67 67 0 0 27 4 2 0 0 0 6.32 16.4 44 9.8 72 72 0 0 24 3 0 1 0 0 116 14 135 3.6 8 5.7 1.19 0.4 0.1 3.1 95 A,C1,P 1929 N 1+ N 7.0 1.0 P 1+ 132 0 0-1 0 1+ 0 0 122 15 145 5.3 - 6.2 1.09 1.0 0.1 3.2 75 L,A, Cl 1520 N Tr N 6.4 1.0 P Tr 260 - 0 0 0 1+ 0 0 Months on Test 12 7.00 16.8 46 9.4 62 62 0 0 32 2 4 0 0 0 7.00 17.4 47 16.9 68 67 1 0 26 3 3 0 0 0 3 6.48 17.4 47 10.9 73 73 0 0 21 6 0 0 0 0 134 16 135 2.9 15 6.0 1.09 0.6 0.3 3.1 123 19 165 3.3 15 6.2 0.73 0.8 0.3 2.6 101 18 150 2.9 14 6.2 0.75 0.8 0.3 2.7 50 A,Cl 1919 N 1+ N 6.2 1.0 P 1+ 242 0 0-2 0 1+ 0 1-5 90 D, Y,Cl 1908 N Tr N 6.4 1.0 P 1+ 222 0 0 0 2+ 0 0 95 D, Y, Cl 1955 N N N 6.8 1.0 P Tr 262 0 0-1 0-1 2+ 0 4-5 ofipany Sanitized. Doss not contain T S C A CBJ Dog No.: 1162 Sex: Male GrouD: Control Hematology Erythrocytes Hemoglobin Hematocrit Leucocytes DIFFERENTIAL Neutrophils Seg. Juv. Myel. Lymphocytes Eosinophils Monocytes Basophils Atypicals Nucleated RBC Biochemistry Glucose Urea Nitrogen Cholesterol Aik. Phos. GPT Total Protein A/G . Creatinine Bilirubin Albumin Urinalysis Volume Appearance Osmolality .Occult Blood Sugar Acetone pH Urobilinogen Bilirubin Protein Creatinine MICROSCOPIC RBC 's WBC 's Epithelial Bacteria Cast Sperm CLINICAL LABORATORY IATA ON INDIVIDUAL DOGS Fpf fcoR THREE MONTHS Control 6.08 15.3 40 14.6 67 67 0 0 27 2 4 0 0 0 6.08 15.6 40 16.8 67 65 2 0 23 8 2 0 0 0 118 9 115 5.0 9 6.2 1.04 0.5 0.1 3.2 114 8 140 4.5 8 6.9 1.06 0.7 0.1 3.6 160 D, Y, C1,P 1556 N N N 8.0 1.0 P 1+ 128 0 0-1 0 1+ 0 0 225 L, A, Cl, P 1514 N N N 7.2 1.0 P Tr 132 0 0 0-1 2+ 0 0-1 Months on Test 12 7.16 17.1 47 15.0 65 65 0 0 22 13 0 0 0 0 7.20 17.2 46 16.9 70 70 0 0 25 4 1 0 0 0 3 6.80 17.1 46 11.3 54 54 0 0 30 15 1 0 0 0 112 10 130 3.9 15 6.1 1.38 0.5 0.3 3.5 118 11 145 3.6 14 6.0 1.00 0.60.2 3.0 106 14 130 3.6 11 6.4 1.03 0.7 0.2 3.2 130 A 5C 1 3P 1971 N N N 7.0 1.0 P 1+ 184 0 1-3 11-J'i 1+ 0 0-2 175 Y,C1SP 1892 N N N 7.2 1.0 P 1+ 190 0 0 0 2+ 0 1-3 210 L, A,C1SP 2133 N N N 7.0 0.1 P 1+ 172 0 0 0-1 1+ 0 3-6 COTti*"1, S a * * . Does nt contain TSCA CBS Dog No.: 1153 Sex: Male Group: 500 ppm Hematology Erythrocytes Hemoglobin Hematocrit Leucocytes DIFFERENTIAL Neutrophils Seg. Juv. Myel. Lymphocytes Eosinophils Monocytes Basophils Atypicals Nucleated RBC Biochemistry Glucose Urea Nitrogen Cholesterol Aik. Phos. GPT Total Protein A/G Creatinine Bilirubin A lbumin Urinalysis Vo lurne Appearance Osmolality Occult Blood Sugar ' Acetone pH Urobilinogen Bilirubin Protein Creatinine MICROSCOPIC RBC 's WBC 1s Epithelial Bacteria Cast Sperm CLINICAL LABORATORY .DATA ON INDIVIDUAL DOGS F E D H ^ H H H f OR THREE MONTHS . Control 7.00 17.3 48 13.3 66 66 0 0 30 4 0 0 0 0 7.68 18.8 50 11.1 60 60 0 0 34 2 4 0 0 0 116 13 140 6.4 9 6.2 1.39 0.4 0.1 3.6 158 14 160 6.1 7 6.5 0.89 0.8 0.1 3.1 245 Yj Cl, P 605 N N N 8.0 0.1 N N 66 0 0 0 3+ 0 0 185 Y,C1,P 1268 N N N 7.0 1.0 P Tr 118 0 0-1 o 2+ 0 0 Months on Test 12 3 7.84 18.7 52 11.0 62 61 1 0 33 2 3 0 0 0 8.08 19.7 53 15.4 62 61 1 0 30 8 0 0 0 0 7.60 18. 7 50 9.9 63 62 1 0 28 7 z 0 0 0 140 21 100 7.8 20 6.3 1.21 0.4 3.4 142 18 170 8.2 18 6.3 1.18 0.9 0.3 3.4 104 23 160 8.6 10 6.6 0.98 0.9 0.4 3.3 215 A, Cl, P 1147 N 'N N 7.0 0.1 P Tr 88 0 0-1 0 3+ 0 1-5 110 Y,C1,P 1567 N N N 7.6 0.1 P 1+ 130 0 0 o 2+ 0 2-5 210 D,Y,C1,P 1331 N N N 6.8 1.0 P Tr 138 0 0 0-1 2+ 0 3-10 Company Sanitized. Does no! contain T S C A CB1 Dog No,: 1155 Sex: Male Grout) : 500 ppm Hematology Erythrocytes Hemoglobin Hematocrit Leucocytes DIFFERENTIAL Neutrophils Seg. Juv. Myel. Lymphocytes Eosinophils Monocytes Basophils Atypicals Nucleated RBC Biochemistry Glucose Urea Nitrogen Cholesterol Aik. Phos. GPT Total Protein A/G Creatinine Bilirubin Albumin Urinalysis Vo lime Appearance Osmolality Occult Blood Sugar Acetone pH Urobilinogen Bilirutin Protein Creatinine MICROSCOPIC RBC 's WBC 's Epithelial Bacteria Cast Sperm CLINICAL LABO! to; )ATA ON INDIVIDUAL DOGS fed! IFOR THREE MONTHS Control ' 5.92 14.9 39 13.0 65 65 0 0 28 6 1 0 0 0 5.68 14.4 37 13.9 62 62 0 0 26 9 3 0 0 0 Months on Test 12 6.56 15.6 42 13.2 66 66 0 0 28 6 0 0 0 0 6.76 16.1 41 12.5 68 67 1 0 28 4 0 0 0 0 3 6.64 15.9 41 9.2 69 69 0 0 28 3 0 0 0 0 112 20 115 7.6 6 5.8 1.00 0.6 0.1 2.9 114 17 135 7.1 4 6.1 1.08 0.8 0.1 3.2 120 26 135 8.1 15 6.0 1.00 0.6 0.5 3.0 126 . 21 160 10.6 13 6.5 1.06 0.8 0.2 3.3 120 11 145 12.0 11 6.1 1.30 0.7 0.1 3.4 195 Y.Cl.P 1478 N N N 7.0 0.1 N Tr 104 0 0 ur\-11 2+ 0 0 245 Y,C1,P 1273 N N N 7.0 1.0 P N 92 0 0-2 0 2+ 0 0 125 A,C15P 1819 N Tr N 7.0 1.0 N 1+ 124 0 0 0 1+ 0 0-1 185 D,Y5C15P 1966 N N N 7.0 1.0 P 1+ 158 0 0 2+ 0 0 155 ]1-5A, Cl,P 2181 N N N 6.8 1.0 P 1+ 208 0 0 0 3+ 0 0-1 Company Sanitized, Does not contain T S C A C B i Dog No.: 1159 Sex: Male Groun: 50 0 nnm Hematology Erythrocytes Hemoglobin Hematocrit Leucocytes DIFFERENTIAL Neutrophils Seg. Juv. Myel. Lymphocytes Eosinophils Monocytes Basophils Atypicals Nucleated RBC Biochemistry Glucose Urea Nitrogen Cholesterol Aik. Phos. GPT Total Protein A/G Creatinine Bilirubin Albumin Urinalysis Volume Appearance Osmolality Occult Blood Sugar Acetone pH Urobilinogen Bilirubin Protein Creatinine MICROSCOPIC RBC 's WBC 's Epithelial Bacteria Cast Sperm CLINICAL LABORATOR^lATA ON INDIVIDUAL DOGS F E D & ^ H ^ H f o r t h r e e m o n t h s Control . ________________________________________________________ -- ----------------- Months on Test 122 5.64 15.2 40 11.0 72 71 1 0 27 1 0 0 0 0 5.60 15.3 39 12.3 60 60 0 0 32 5 3 0 0 0 5.84 14.9 39 12.6 60 58 2 0 31 5 4 0 0 0 5.48 14.3 36 13.7 75 75 0 0 17 6 2 0 0 0 5.60 14.3 36 12.9 71 70 1 0 23 6 0 0 0 0 116 18 165 6.6 7 6.2 0.92 0.5 0.1 3.0 124 17 175 6.4 6 6.3 1.48 0.9 0.1 3.8 118 ` 17 175 7.5 9 6.1 1.40 0.5 0.2 3.6 118 17 195 8.7 13 6.0 1.13 0.8 0.1 3.2 109 19 185 8.3 12 6.1 0.93 0.9 0.2 2.9 350 L,Y,C1,P 560 N N N 6.2 0.1 N N 52 0 0 Urv 2+ .0 0 165 Y,C1SP 1121 N N N 7.2 1.0 P Tr 130 0 0 0 3+ 0 0 185 D,A,C1,P 1945 N Tr N 7.4 1.0 P 1+ 168 0 0 0-1 2+ 0 0-1 235 A,C1,P 1929 N Tr N 7.0 0.1 P Tr 182 0 0 0 2+ 0 0-1 275 L, A, Cl, P 1656 N N N 7.0 1.0 P Tr 170 0 5-10 0-1 2+ 0 0-2 Company Sanitized. Does not contain T S C A CBf Dog No.: 1165 Sex: Male Group :___ 500 ppm Hematology Erythrocytes Hemoglobin Hematocrit Leucocytes DIFFERENTIAL Neutrophils Seg. Juv. Myel. Lymphocytes Eosinophils Monocytes Basophils Atypicals Nucleated RBC Biochemistry Glucose Urea Nitrogen Cholesterol Aik. Phos. GPT Total Protein A/G Creatinine Bilirubin Albumin Urinalysis Volume Appearance Osmolality Occult Blood Sugar Acetone pH Urobilinogen Bilirubin Protein Creatinine MICROSCOPIC RBC's WBC's Epithelial Bacteria Cast Sperm CLINICAL LABQRAIOB^JA.TA ON INDIVIDUAL DOGS FED 'FOR THREE MONTHS f BMT Control 4.96 13.2 33 10.4 60 60 0 0 38 1 0 1 0 0 5.92 15.7 41 16.2 64 64 0 0 35 1 0 0 0 0 134 12 126.5 6.2 1.11 0.2 3.3 150 16 135 4.8 7 6.2 1.35 0.9 0.1 3.6 200 L,A,Cl, P 1283 N N N 7.2 0.1 P Tr 114 0 0-1 0-1 3+ 0 0 240 Y,Cl, P 1136 N N' N 6.8 0.1 P Tr 92 0 0 0 1+ 0 0 Months on Test______ , 1 2__________ 3 7.24 17.6 49 15.0 62 62 0 0 35 3 0 0 0 0 6.48 16.6 44 13.2 69 69 0 0 30 0 1 0 0 0 6.32 16.3 44 12.2 66 66 0 0 27 7 0 0 0 0 182 23 150 4.2 13 5.5 1.03 0.6 0.3 2.8 116 18 170 4.8 14 "6.1 1.22 0.8 0.2 3.4 116 25 160 4.0 12 6.1 0.91 0.9 0.2 2.9 310 Y, Cl 1016 N N N 6.8 0.1 P N 76 0 0 0 3+ 0 0 190 A, Cl, P 1698 N N N 7.0 1.0 P 1+ 142 0 0-1 0 2+ 0 0-3 65 Y, Cl 1383 N N N 7.0 0.1 P Tr 116 0 0-3 0 2+ 0 0-1 Company Sanitized. Does not contain Y S C CSt Dog No.: 1152 Sex: Male Grout) : 750 ppm Hematology Erythrocytes Hemoglobin Hematocrit Leucocytes DIFFERENTIAL Neutrophils Seg. Juv. Myel. Lymphocytes Eosinophils Monocytes Basophils Atypicals Nucleated RBC Biochemistry Glucose Urea Nitrogen Cholesterol Aik. Phos. GPT Total Protein A/G Creatinine Bilirubin Albumin Urinalysis Volume Appearance Osmolality Occult Blood Sugar Acetone pH Urobilinogen Bilirubin Protein Creatinine MICROSCOPIC RBC 's WBC 's Epithelial y Bacteria Cast Sperm AX XXU11X/J-A CLINICAL Li [ORATORY DATA ON INDIVIDUAL DOGS FED ~If o r THREE MONTHS Control 6.56 16.9 45 8.9 69 68 1 0 26 2 3 0 0 0 6.80 17.0 45 8.2 66 65 1 0 31 0 3 0 0 0 112 16 150 4.2 7 6.4 1.23 0.5 0.1 3.5 120 24 165 4.4 6 6.4 1.05 1.1 0.1 3.3 105 A, Cl,P 1850 N N N 7.2 0.1 P 1+ 236 0 0 0 2+ 0 0 150 D,Y, Cl, P 1987 N N N 7.0 1.0 P Tr 180 0 0 0 2+ 0 0-1 Months on Test 12 7.04 17.1 48 10.9 78 78 0 0 22 0 0 0 0 0 7.36 17.9 49 13.6 74 74 0 0 25 1 0 0 0 0 3 7.08 1/. 8 49 11.8 68 68 0 0 28 1 3 0 0 0 116 28 185 8.1 15 6.1 1.55 0.6 0.2 3.7 120 23 215 8.3 21 6.3 1.28 0.9 0.3 3.5 in 19 195 6.5 19 6.2 0.80 1.0 0.2 2.8 110 A,Cl 2120 N Tr N 6.4 1.0 P Tr 180 0 0 0-1 2+ 0 0 135 D, Y, Cl, P 2420 N __ Tr N 6.8 1.0 P 1+ 182 0 0-4 0 2+ 0 0-1 180 D,Y,C1,P 1861 N Tr N 7.0 1.0 P 1+ 180 0 0-1 0-2 2+ 0 0 Company Sanitized. Does not contain T S C A CBI CLINICAL LABORATORY .DATA ON INDIVIDUAL DOGS f e d A ^ M A f o r THREE MONTHS Dog No.: 1156 Sex: Male Group :____ 750 ppm__________________________________ ________ _________ Months on Test Hematology Erythrocytes Hemoglobin Hematocrit Leucocytes DIFFERENTIAL Neutrophils Seg. Juv. Myel. Lymphocytes Eosinophils Monocytes Basophils Atypicals Nucleated RBC Control 5.68 14.1 36 11.1 66 65 1 0 32 2 0 0 0 0 6.12 15.9 42 10.5 64 64 0 0 34 1 1 0 0 0 1 6.56 16.1 43 12.9 61 61 0 0 32 3 4 0 0 0 2 6.52 15.7 42 11.4 57 57 0 0 29 12 2 0 0 0 3 6.40 16.0 43 11.7 65 65 0 0 26 9 0 0 0 0 Biochemistry Glucose Urea Nitrogen Cholesterol Aik. Phos. GPT Total Protein A/G Creatinine Bilirutin Albumin 106 17 160 3.7 7 5.9 0.93 0.6 0.1 2.8 104 15 180 3.5 4 6.0 0.79 0.9 0.1 2.6 110 24 180 3.7 16 5.6 1.09 0.5 0.5 2.9 104 19 190 4.2 17 5.7 1.09 0.7 0.1 3.0 109 15 180 3.3 18 5.9 1.27 0.9 0.1 3.3 Urinalysis Volume Appearance Osmolality Occult Blood Sugar Acetone pH Urobilinogen Bilirubin Protein Creatinine MICROSCOPIC RBC 1s WBC's Epithelial Bacteria Cast Sperm 140 Y,Cl,P 1924 N Tr N 6.8 1.0 N Tr 138 0 2-6 0 2+ 0 1-2 01 h--* 255 L,A) Cl, P 1777 N N N 7.6 0.1 P Tr 116 0 0 0 2+ 0 125 A, Cl, P 2045 .N Tr N 6.8 1.0 P 1+ 130 0 0-2 0-1 1+ 0 0 90 A,Cl,P 1971 N Tr N 7.0 1.0 P 1+ 196 0 0-2 0 2+ 0 4-8 90 D,Y, Cl 2207 N Tr N 6.8 1.0 P 1+ 224 0 2-5 0-1 2+ 0 1-6 Company Sanitized. Does not contain T S C A C B f Dog No.: 1160 Sex: Male Group: 750 ppm Hematology Erythrocytes Hemoglobin Hematocrit Leucocytes DIFFERENTIAL Neutrophils Seg. Juv. Myel. Lymphocytes Eosinophils Monocytes Basophils Atypicals Nucleated RBC Biochemistry Glucose Urea Nitrogen Cholesterol Aik. Phos. GPT Total Protein A/G Creatinine Bilirubin Albumin Urinalysis Volume Appearance Osmolality Occult Blood Sugar Acetone pH Urobilinogen Bilirubin Protein Creatinine MICROSCOPIC RBC 's WBC 's Epithelial Bacteria Cast Sperm j- ^j-j-j CLINICAL IABOEATOR^ATA ON INDIVIDUAL DOGS f e l m H H I H f o r THREE MONTHS Control _____ _______ ________________________ Months on Test 12 3 ' 5.92 15.5 39 8.1 69 69 0 0 29 1 1 0 0 0 5.68 15.7 42 8.5 67 67 0 0 30 2 1 0 0 0 6.20 16.3 43 9.6 74 73 1 0 25 0 1 0 0 0 6.44 16.9 42 8.9 70 70 0 0 29 1 0 0 0 0 6.40 17.2 46 14.4 80 80 0 0 18 A U 0 0 0 108 11 160 5.2 .. . 8 5.8 1.23 0.4 0.1 3.2 112 6 180 5.3 6 6.4 1.62 0.7 0.1 4.0 104 . 19 200 . 7.2 13 5.8 1.06 0.4 0.2 3.0 112 15 230 8.0 16 6.2 1.22 0.7 0.2 3.4 104 20 200 6.1 16 6. 3 0.88 0.8 0.2 2.9 205 Y, Cl, P 1215 N N N 7.0 0.1 P Tr 112 0 0 0 1+ 0 0-1 170 Y,Cl,P 1289 N N N 7.0 1.0 P Tr 130 0 0 0 2+ 0 0 115 A, Cl 1992 N Tr N 6.8 0.1 P Tr 140 0 0-1 0 2+ 0 0-2 70 D,Y,Cl, P 1877 N Tr N 7.2 0 .1 P Tr 196 0 0 0 2+ 0 0 130 L,A, Cl, P 2151 N N N 6.8 1.0 P 1+ 180 0 0 0-1 2+ 0 0-1 ^ n p j p i P ^ r?',OR pn^ n TSCA c m A X X XJX'IX/.XA X. \ XX- J CLINICAL LABORATORY DATA ON INDIVIDUAL DOGS f e d I M K o r three MONTHS Dog No.: 1164 Sex: Male Group: 750 ppm ____________________ _____________________ ______________ ________ Months on Test Hematology Erythrocytes Hemoglobin Hematocrit Leucocytes DIFFERENTIAL Neutrophils Seg. Juv. My el. Lymphocytes Eosinophils Monocytes Basophils Atypicals Nucleated RBC Control 6.24 16.8 44 17.1 59 59 0 0 32 5 4 0 0 0 5.76 15.1 40 16.3 62 62 0 0 29 9 0 0 0 0 ____ 1__________ 2_____ 6.40 15.7 42 18.3 67 67 0 0 27 6 0 0 0 0 6.28 16.0 42 16.2 66 64 2 0 20 14 0 0 0 0 6.56 16.7 44 14.6 64 64 0 0 25 11 0 0 0 0 Biochemistry Glucose Urea Nitrogen Cholesterol Aik. Phos. GPT Total Protein A/G Creatinine Bilirubin Albumin 114 11 140 4.2 8 5.8 1.04 0.4 0.1 3.0 114 9 155 4.4 15 6.3 1.00 0.8 0.1 3.2 114 12 170 7.4 14 6.1 1.29 0.5 0.2 3.4 127 12 190 7.2 17 6.1 1.12 0.6 0.1 3.2 110 11 180 5.3 15 6.2 1.13 0.7 0.1 3.3 Urinalysis Volume Appearance Osmolality Occult Blood Sugar Acetone pH Urobilinogen Bilirutin Protein Creatinine MICROSCOPIC R B C 's WBC's Epithelial Bacteria Cast Sperm 225 D,Y,C1,P 1373 N N N 7.0 0.1 P Tr 132 0 0 0 1+ 0 0 1 O 170 Yj Cl,P 1478 N N N 7.4 1.0 N N 118 0 0 0 2+ 0 185 Y, Cl,P 1341 N Tr N 7.0 1.0 P 1+ 96 0 0-1 Ur\ 3+ 0 160 D,Y, C1,P 1535 N N N 7.2 0.1 P Tr 142 65 Y, Cl 1404 N N N 6.8 1.0 P 1+ 182 00 0 0-1 0 0-1 2+ 4+ 00 copain T S A C m Dog No.: 1151 Sex: Male Group :___ 2500 ppm Hematology Erythrocytes Hemoglobin Hematocrit Leucocytes DIFFERENTIAL Neutrophils Seg. Juv. Myel. Lymphocytes Eosinophils Monocytes Basophils Atypicals Nucleated RBC Biochemistry Glucose Urea Nitrogen Cholesterol Aik. Phos. GPT Total Protein A/G Creatinine Bilirubin Albumin Urinalysis Vo lume Appearance Osmolality ^ Occult Blood Sugar Acetone pH Urobilinogen Bilirubin Protein Creatinine MICROSCOPIC RBC 's WBC's Epithelial Bacteria Cast Sperm AX UIVX'XA X ^i--J J CLINICAL LAB0RAT0RYJ1A'TA ON INDIVIDUAL DOGS FEdD H H H H fiOR THREE MONTHS U Ji Control 6.64 17.2 46 13.0 72 71 1 0 23 5 0 0 0 0 6.48 17.3 45 12.1 62 62 0 0 37 1 0 0 0 0 92 18 150 5.5 5 6.6 1.07 0.4 0.2 3.4 114 14 155 5.4 4 6.1 1.04 0.8 0.1 3.1 260 ,A,Cl, P 1651 N N N 7.0 0.1 P Tr 128 0 0 0 2+ 0 .0 130 D,Y, Cl, P 1745 N. N N 7.6 1.0 P Tr 154 0 0-1 0 2+ 0 0 Months on Test 12 7.00 16.5 45 16.7 6.68 16.4 43 16.5 67 67 64 67 30 00 26 27 75 01 00 00 00 3 6.20 16.3 43 21.9 80 77 3 0 14 5 1 0 0 0 94 21 185 10.8 10 5.9 1.05 0.6 0.2 3.0 108 24 230 13.6 12 6.3 0.78 0.7 02.. 81 102 10 200 12.2 9 6.2 1.21 0.7 0.1 3.4 105 Y, Cl 1730 N N N 7.0 1.0 P Tr 108 0 0-1 0 3+ 0 0-1 125 A,Cl,P 1908 N N N 7.0 1.0 P Tr 168 0 0 0 2+ 0 0-1 100 D,Y, Cl, P 1824 N N N 6.8 1.0 P Tr 220 0 0-3 o-i 4+ 0 0 o s s Fis? esnaTr! T S 'CACW XXXXJXXt CLINICAL LAORAT FEDI J- V.J.-TJ TA ON INDIVIDUAL DOGS 'OR THREE MONTHS Dog No.: 1157 Sex: Male Group: 2500 p p m ______________________________________ ________________ Months on Test Hematology Erythrocytes Hemoglobin Hematocrit Leucocytes DIFFERENTIAL Neutrophils Seg. Juv. Myel. Lymphocytes Eosinophils Monocytes Basophils Atypicals Nucleated RBC Control 6.68 16.9 44 12.8 78 78 0 0 22 0 0 0 0 0 6.48 16.5 44 10.8 73 72 1 0 25 2 0 0 0 0 1 6.92 16.8 45 11.8 67 67 0 0 26 7 0 0 0 0 2 6.56 16.0 42 13.1 73 73 0 0 2 4 3 0 0 0 3 5.76 15.1 40 13.8 68 68 0 0 23 9 0 0 0 0 Biochemistry Glucose Urea Nitrogen Cholesterol Aik. Phos. GPT Total Protein A/G Creatinine Bilirubin Albumin 116 25 145 4.1 10 5.8 1.03 0.6 0.1 2.9 114 18 145 4.2 4 5.9 1.02 0.9 0.1 3.0 94 27 .170 12.6 11 6.3 1.03 0.6 0.3 3.2 122 17 185 10.1 14 6.0 1.11 0.7 . 0.1 3.2 103 22 170 11.7 12 6.2 1.08 0.7 0.1 3.2 Urinalysis Volume Appearance Osmolality Occult Blood Sugar Acetone pH Urobilinogen Bilirubin .Protein Creatinine MICROSCOPIC RBC's WBC 's Epithelial Bacteria Cast Sperm 240 A, Cl, P 2138 N N N 7.2 0.1 P Tr 136 0 0 0 2+ 0 0 150 Y, Cl, P 1619 N N N 6.8 0.1 P N 122 0 0 0-1 1+ 0 0 t"1-l O 175 A, Cl, P 1961 N N N 7.0 0.1 N Tr 130 0 0-1 0 2+ 0 1-3 90 A,C1,P 1814 N N N 7.2 1.0 P Tr 226 0 0-1 0 3+ 0 180 D,Y,Cl,P 1567 N N N 7.2 1.0 P Tr 144 0 0-2 0-1 3+ 0 0-4 Com pany Sanize. Does noi conan T S C A OBI Dog No.: 1161 Sex: Male Group: 2500 ppm Hematology Erythrocytes Hemoglobin Hematocrit Leucocytes DIFFERENTIAL Neutrophils Seg. Juv. Myel. Lymphocytes Eosinophils Monocytes Basophils Atypicals Nucleated RBC CLINICAL L ABMORATORY|ATA ON INDIVIDUAL DOGS FE FOR THREE MONTHS Control 6.64 17.0 44 8.3 61 61 0 0 36 3 0 0 0 0 6.60 16.8 44 8.9 56 56 0 0 35 8 1 0 0 0 Months on Test 12 6.68 15.8 42 12.3 66 64 2 0 22 11 1 0 0 0 6.12 15.1 39 10.8 65 65 0 0 32 3 0 0 0 0 3 6.52 15.7 42 11.6 67 67 0 0 27 5 1 0 0 0 Biochemistry Glucose Urea Nitrogen Cholesterol Aik. Phos. GPT Total Protein A/G Creatinine Bilirubin Albumin 119 20 130 3.7 9 6.3 1.08 0.5 0.1 3.3 120 12 140 3.6 8 6.1 1.18 0.7 0.1 3.3 105 30 160 8.6 12 6.2 1.08 0.6 0.3 3.2 112 22 190 8.7 17 6.4 1.09 0.7 0.2 3.3 100 27 180 10.1 14 6.4 1.28 0.8 0.2 3.6 Urinalysis Volume Appearance Osmolality Occult Blood Sugar Acetone pH Urobilinogen Bilirubin Protein Creatinine MICROSCOPIC RBC 1s WBC 's Epithelial Bacteria Cast Sperm 140 D,Y,C1,P 1961 P N N 6.8 1.0 N Tr 156 0 0-1 0 2+ 0 0-1 120 L, A,Cl, P 2272 N N N 7.0 1.0 N Tr 174 0 2-6 0 1+ 0 0 180 A, Cl 1955 N -N N 6.8 1.0 P Tr 120 0 2-10 0-1 2+ 0 0-2 150 D, Y, Cl,P 2164 N N N 7.0 1.0 P 1+ 152 0 1-5 0 2+ 0 0 170 D,Y, Cl 2103 N N N 6.8 1.0 P Tr 150 0 0-3 0-2 2+ 0 5-10 ompany Sanitized. Does not contain T S C A CBI CLINICAL LABORATORI lATA ON INDIVIDUAL DOGS FEDI FOR THREE MONTHS Dog No.: 1163 Sex: Male Group : 2500 ppm Hematology Erythrocytes Hemoglobin Hematocrit Leucocytes DIFFERENTIAL Neutrophils Seg. Juv. ' Myel. Lymphocytes Eosinophils Monocytes Basophils Atypicals Nucleated RBC Biochemistry Glucose Urea Nitrogen Cholesterol Aik. Phos. GPT Total Protein A/G Creatinine Bilirubin Albumin Urinalysis Volume Appearance Osmolality Occult Blood Sugar Acetone pH Urobilinogen Bilirubin Protein Creatinine MICROSCOPIC RBC 1s WBC 's Epithelial Bacteria Cast Sperm Control 5.12 13.1 33 12.4 76 75 1 0 23 1 0 0 0 0 5.24 13.7 35 12.8 67 66 1 0 27 6 0 0 0 0 112 13 150 5.0 9 5.6 0.98 - 0.1 2.8 124 12 165 3.8 9 5.9 1.18 0.7 0.1 3.2 630 L 5Y,C1,P 248 N N N 7.0 0.1 N N 18 0 0-5 0 4+ 0 0 460 l ,Y.Cl.P 471 N 'N N 7.0 0.1 N N 38 0 0-2 0 3+ 0 0 Months on Test 12 5.76 13.8 36 11.8 63 62 1 0 31 6 0 0 0 0 6.08 14.6 38 13.4 68 68 0 0 24 6 2 0 0 0 3 5.88 14.2 37 14.1 73 70 3 0 21 6 0 0 0 0 110 14 210 10.8 116.2 1.02 ` 0.6 0.2 3.1 127 *' 20 220 12.7 16 6.4 0.94 0.8 0.5 3.1 122 18 200 12.8 15 6.3 0.95 0.8 0.5 3.1 190 L,Y,C1 352 N N N 7.6 0.1 N N 34 0 0-1 0-1 4+ 0 0 270 y 3c i 5p 718 N N N 7.2 0.1 N Tr 84 0 0-2 0 3+ 0 0 345 y ,c i 5p 570 N N N 7.0 0.1 P N 66 0 0 0-1 4+ 0 0 Company Sanitized. Does not contain T S C A CBS uniiMlUlG UJM INDIVIDUAL DUGS THREE MONTHS Dog No.: 1137 Sex: Female Group: Control Hematology Erythrocytes Hemoglobin Hematocrit Leucocytes DIFFERENTIAL Neutrophils Seg. Juv. Myel. Lymphocytes Eosinophils Monocytes Basophils Atypicals Nucleated RBC Biochemistry Glucose Urea Nitrogen Cholesterol Aik. Phos. GPT Total Protein A/G Creatinine Bilirubin Albumin Urinalysis Vo lume Appearance Osmolality Occult Blood Sugar Acetone pH Urobilinogen Bilirubin Protein Creatinine MICROSCOPIC RBC 's WBC 's Epithelial Bacteria Cast . Control 6.72 17.8 47 12.4 60 58 2 0 28 12 0 0 0 0 6.68 18.3 48 11.4 62 62 0 0 25 10 3 0 0 0 114 10 160 4.5 7 6.4 1.20 0.5 0.1 3.5 109 12 150 4.7 3 5.5 1.15 0.5 0.2 2.9 125 D,Y,C1,P 1359 N N N 8.5 1.0 P Tr 146 0 0 0-1 3+ 0 235 D,Y, Cl, P 1651 N N N 6.8 1.0 N Tr 114 0 0-1 0 1+ 0 Months on Test 12 7.40 18.5 51 13.1 76 76 0 0 20 4 0 0 0 0 7.28 18.9 51 10.6 71 71 0 0 24 5 0 0 0 0 3 7.76 19.7 54 9.1 66 66 0 0 25 9 0 0 0 0 111 16 160 4.1 8 6.3 1.50 0.5 0.2 3.8 110 19 185 3.9 9 6.4 1.21 0.7 0.2 3.5 101 26 165 10.1 15 6.7 1.06 0.8 0.2 3.4 165 A, Cl, P 2142 N Tr N 6.8 1.0 P Tr 164 - 0 2-8 0 2+ 0 190 A, Cl, P 2013 N N N 7.0 1.0 P Tr 168 0 0 0 2+ 0 140 A, Cl, P 1646 N N N 6.8 1.0 P 1+ 156 0 0 0 2+ 0 Company Sanitized. Poes not contain T S C A C B I CLINICAL ON INDIVIDUAL DOGS FEd S H I H f OR THREE MONTHS Dog No.: 1141 Sex: Female Group: Control Hematology Erythrocytes Hemoglobin Hematocrit Leucocytes DIFFERENTIAL Neutrophils Seg. Juv. Myel. Lymphocytes Eosinophils Monocytes Basophils Atypicals Nucleated RBC Control 6.52 17.5 45 10.4 72 72 0 0 21 6 1 0 0 0 6.60 17.6 46 11.1 74 71 3 0 15 8 3 0 0 0 Biochemistry Glucose Urea Nitrogen Cholesterol Aik. Phos. GPT Total Protein A/G Creatinine Bilirubin Albumin . 114 10 145 5.0 10 6.3 0.92 0.5 0.1 3.0 112 15 140 5.3 3 6.5 0.95 0.7 0.3 3.2 Urinalysis Volume Appearance Osmolality Occult Blood Sugar Acetone pH Urobilinogen Bilirubin Protein Creatinine MICROSCOPIC RBC 's WBC's Epithelial Bacteria Cast 250 D,Y,C1,P 1546 N N N 7.0 1.0 N Tr 144 0 0-1 0-2 2+ 0 190 L,A,C1,P 2018 N N* N 6. 6 1.0 N Tr 152 0 0 0-1 1+ 0 Months on Test 12 6.00 14.9 39 9.0 73 73 0 0 26 1 0 0 0 0 6.64 17.2 45 10.8 77 75 2 0 20 2 1 0 0 0 3 6.28 16.2 43 7.8 65 65 0 0 27 6 2 0 0 0 112 17 190 4.1 12 6.3 1.10 0.6 0.2 3.3 147 11 160 3.6 19 6.3 1.23 0.9 0.1 3.5 112 16 145 4.3 13 6.2 1.50 0.8 0.1 3.7 150 D,Y, Cl, P 1625 N Tr N 7.0 1.0 N Tr 142 0 0 0-3 3+ 0 85 D,Y, Cl, P 1924 N N N 8.0 1.0 P Tr 230 0 0-1 0-1 3+ 0 175 A,Cl, P 1856 N N N 7.0 1.0 N Tr 174 0 0-2 1-5 2+ 0 Gcompany Sanitised. Does not contain T S C A CB Dog No.: 1145 Sex: Female Group: Control Hematology Erythrocytes Hemoglobin Hematocrit Leucocytes DIFFERENTIAL Neutrophils Seg. Juv. Myel. Lymphocytes Eosinophils Monocytes Basophils Atypicals Nucleated RBC Biochemistry Glucose Urea Nitrogen Cholesterol Aik. Phos. GPT Total Protein A/G Creatinine Bilirutin Albumin Urinalysis Vo lume Appearance Osmolality Occult Blood Sugar Acetone pH Urobilinogen Bilirubin Protein Creatinine MICROSCOPIC RBC's WBC's Epithelial Bacteria Cast CLINICAL Li ORATORY AIA ON INDIVIDUAL DOGS fe d ] FOR THREE MONTHS Control 7.08 16.9 43 9.2 74 74 0 0 24 2 0 0 0 0 6.72 16.0 41 11.2 64 63 1 0 30 3 3 0 0 0 106 13 155 3.5 7 5.9 1.16 0.7 0.6 3.2 118 11 175 3.4 6 5.9 1.47 0.8 0.2 3.5 190 Y, Cl, P 1289 N N N 7.6 1.0 N N 128 0 0 0-1 2+ 0 80 A, Cl, P 2303 N N N 8.0 1.0 N Tr 236 0 0-1 0 1+ 0 Months on Test 12 6.88 16.2 43 9.5 78 78 0 0 20 2 0 0 0 0 7.20 17.8 47 12.6 74 74 0 0 26 0 0 0 0 0 3 6.76 16.8 42 8.3 66 65 1 0 33 1 0 0 0 0 110 14 155 3.5 10 5.4 1.58 0.6 0.2 3.3 118 . 15 160 3.2 17 5.7 0.99. 0.8 0.2 2.8 102 21 295 3.1 13 6.5 1.32 1.0 0.2 3.7 110 A,Cl,P 1824 N Tr N 7.2 1.0 P Tr 186 0 0 0-1 2+ 0 120 D,Y, C1,P 1871 N N 7.0 1.0 P 1+ 196 0 0 0-1 2+ 0 175 L,A, Cl,P 1735 N Tr N 7.0 1.0 P 1+ 180 0 0 0 1+ 0 ipany Sanitized. Does not contain T S C A CBS Dog No.: 1147 Sex: Female Control Hematology Erythrocytes Hemoglobin Hematocrit Leucocytes DIFFERENTIAL Neutrophils Seg. Juv. Myel. Lymphocytes Eosinophils Monocytes Basophils Atypicals Nucleated RBC Biochemistry Glucose Urea Nitrogen Cholesterol Aik. Phos. GPT Total Protein A/G Creatinine Bilirubin Albumin Urinalysis Vo lume Appearance Osmolality Occult Blood Sugar ' Acetone pH Urobilinogen Bilirubin Protein Creatinine MICROSCOPIC RBC 1s WBC 1s Epithelial Bacteria Cast CLINICAL Li [ORATORY DATA ON INDIVIDUAL DOGS FED If o r THREE MONTHS Control 6.32 16.1 43 11.8 61 61 0 0 27 11 1 0 0 0 6.52 17.1 44 11.5 60 60 0 0 30 6 4 0 0 0 Months on Test 12 6.16 15.5 43 17.2 60 60 0 0 34 5 1 0 0 0 6.60 17.1 45 15.2 66 66 0 0 28 5 1 0 0 0 3 5.84 17.4 46 12.8 60 59 1 0 24 16 0 0 0 0 114 20 170 4.4 8 6.8 1.04 0.8 0.1 3.5 110 22 170 3.9 5 6.4 1.02 1.1 0.1 3.2 100 36 150 4.0 6 5.8 0.98 1.0 0.2 2.9 116 41 165 3.6 10 5.7 0.94 1.2 0.1 2.8 109 46 175 3.1 8 5.8 1.27 1.8 0.1 3.2 150 Y,Cl,P 1073 N N N 6.8 0.1 P Tr 152 0 0-2 0-1 2+ 0 50 D,Y,C1 1945 N N N 7.0 1.0 N Tr 340 0 0 0-2 2+ 0 80 D,Yj Cl 2008 N ' Tr N 6.4 1.0 P Tr 208 140 D,Y, Cl 1367 N N N 7.2 0.1 P Tr 138 110 D,Y, Cl 1499 N N N 7.0 1.0 P Tr 170 0 00 o 00 0 00 1+ 2+ 2+ 0 00 Company Sanitized. Does not contain T S C A CBI Dog No.: 1135 Sex: Female Group ;___ 500 ppm Hematology Erythrocytes Hemoglobin Hematocrit Leucocytes DIFFERENTIAL Neutrophils Seg. Juv. Myel. Lymphocytes Eosinophils Monocytes Basophils Atypicals Nucleated RBC Biochemistry Glucose Urea Nitrogen Cholesterol Aik. Phos. GPT Total Protein A/G Creatinine Bilirubin Albumin Urinalysis Volume Appearance Osmolality Occult Blood Sugar Acetone pH _ Urobilinogen Bilirubin Protein Creatinine MICROSCOPIC RBC 's WBC 's Epithelial Bacteria Cast vjuj.ivj.oa.ij FE TA ON INDIVIDUAL DOGS OR THREE MONTHS Control 7-04 18.8 49 10.9 66 66 0 0 32 1 1 0 0 0 5.68 14.6 47 11.2 72 71 1 0 25 0 3 0 0 0 Months on Test 12 7.08 17.8 48 11.4 71 69 2 0 24 5 0 0 0 0 7.24 18.0 48 12.4 74 72 2 0 24 2 0 0 0 0 3 6.64 16.5 43 11.2 60 58 2 0 22 16 2 0 0 0 102 13 130 7.0 6 6.7 1.40 0.5 0.2 3.9 110 8. 150 6.6 2 5.8 1.09 0.5 0.2 3.0 118 11 205 8.0 9 5.8 1.05 0.6 0.3 3.0 ' 104 23 235 6.5 10 6.6 0.90 0.7 0.4 3.1 110 22 285 4.2 9 6.8 0.80 0.8 0.3 3.0 60 D,Y,C1,P 1871 N Tr .N 66 0.1 N Tr 202 0 0-1 0-2 1+ 0 165 Y,C1,P 1136 N N N 7.0 0.1 N Tr 88 0 0-2 0 1+ 0 160 A,Cl,P 1467 N 1+ N 8.0 1.0 P 1+ 118 0 0-1 0-5 2+ 0 160 D, Y, Cl, P 1488 N Tr ' N 7.0 1.0 N Tr 154 0 0 0-1 2+ 0 145 A,Cl 2098 N N N 6.8 1.0 P Tr 182 0 0 0 1+ 0 ponipany Sanitized. Does ni contain TSCACBP Dog No.: 1140 Sex: Female Group ; 500 ppm Hematology Erythrocytes Hemoglobin Hematocrit Leucocytes DIFFERENTIAL Neutrophils Seg. Juv. Myel. Lymphocytes Eosinophils Monocytes Basophils Atypicals Nucleated RBC Biochemistry Glucose Urea Nitrogen Cholesterol Aik. Phos. GPT Total Protein A/GCreatinine Bilirubin Albumin Urinalysis Vo lume Appearance Osmolality Occult Blood Sugar ' Acetone PH Urobilinogen Bilirubin Protein Creatinine MICROSCOPIC RBC 1s WBC's Epithelial Bacteria Cast CLINICAL FED lATA ON INDIVIDUAL DOGS OR THREE MONTHS Control 6.20 15.5 41 8.2 62 61 1 0 34 3 0 1 0 0 6.40 16.5 44 7.3 72 71 1 0 26 2 0 0 0 0 Months on Test 12 6.44 16.4 45 10.5 61 61 0 0 33 4 2 0 0 0 6.68 17.1 45 11.1 69 69 0 0 26 5 0 0 0 0 3 6.16 15.7 41 7.7 68 68 0 0 25 7 0 0 0 0 119 10 170 10.7 7 6.1 1.25 0.8 0.1 3.4 122 9 170 10.9 3 6.0 1.30 0.6 0.1 3.4 120 17 165 8.3 10 5.9 1.15 0.5 0.3 3.2 110 22 170 6.9 9 6.1 1.12 0.6 0.3 3.2 117 17 200 4.7 6 6.1 1.54 0.7 0.3 3.7 485 L,Y, Cl, P 209 N N N 7.2 0.1 N N 14 0 0-2 0-2 3+ 0 165 Y,Cl,P 951 N N N 8.0 0.1 N N 74 0 0 0-1 2+ 0 100 D, Y,Cl 1310 N ' Tr N 7.2 1.0 N Tr 102 0 0-2 0 1+ 0 90 A,Cl 2151 N Tr .N 7.2 1.0 P 1+ 204 0 0-1 0 1+ 0 85 D,Y, Cl, P 1814 N N N 7.2 1.0 P 1+ 196 0 0 0-1 2+ 0 Dog No.: 1143 Sex: Female Group : 500 ppm Hematology Erythrocytes Hemoglobin Hematocrit Leucocytes DIFFERENTIAL Neutrophils Seg. Juv. Myel. Lymphocytes Eosinophils Monocytes Basophils Atypicals Nucleated RBC Biochemistry Glucose Urea Nitrogen Cholesterol Aik. Phos. GPT Total Protein A/G Creatinine Bilirubin Albumin Urinalysis Volume Appearance Osmolality Occult Blood Sugar Acetone pH Urobilinogen Bilirubin Protein Creatinine MICROSCOPIC RBC 1s WBC's Epithelial Bacteria Cast FEDICLINICAL L 'ORATORY )ATA ON INDIVIDUAL DOGS [f o r THREE MONTHS Control 5.84 14.3 36 16.1 70 68 2 0 24 1 5 0 0 0 6.28 15.8 41 14.8 74 73 1 0 17 5 4 0 0 0 Months on Test .1 2 5.72 13.8 37 11.3 68 68 0 0 30 2 0 0 0 0 6.40 15.9 40 15.5 60 60 0 0 31 9 0 0 0 0 3 5.92 15.7 41 11.3 67 67 0 0 29 4 0 0 0 0 116 11 145 3.7 7 6.1 1.12 0.5 0.1 3.2 112 12 160 4.7 4 6.4 1.05 0.8 0.1 3.3 93 32 170 6.5 11 5.7 0.97 0.6 0.3 2.8 106 32 200 5.4 9 6.6 0.99 0.7 0.8 3.3 109 33 220 4.1 11 6.6 1.34 0.9 0.9 3.8 150 Y.Cl.P 1205 N N N 7.0 1.0 N Tr 144 0 0 0 2+ 0 * 140 Y, Cl, P 1079 N N N 6.8 1.0 N Tr 146 0 0 0-1 1+ 0 170 D,Y, Cl 1320 N N N 6.8 1.0 N Tr 102 0 0-1 0 3+ 0 150 Y SC1,P 1714 N N N 6.8 1.0 P Tr 146 0 0 nu 4+ 0 205 D,Y, C1,P 1252 N 'N N 7.0 0.1 P Tr 114 0 0 0 1+ 0 Com pany Sanitized. Does not contain T S C A CBl Dog No.: 1149 Sex: Female Group :___ 500 ppm Hematology Erythrocytes Hemoglobin Hematocrit Leucocytes DIFFERENTIAL Neutrophils Seg. Juv. Myel. Lymphocytes Eosinophils Monocytes Basophils Atypicals Nucleated RBC Biochemistry Glucose Urea Nitrogen Cholesterol Aik. Phos. GPT Total Protein A/G Creatinine ' Bilirubin Albumin Urinalysis Volume Appearance Osmolality Occult Blood Sugar Acetone pH Urobilinogen Bilirubin Protein Creatinine MICROSCOPIC RBC 's WBC's Epithelial Bacteria Cast . LililJNXUALi FED] ln TA ON INDIVIDUAL DOGS OR THREE MONTHS Control 6.64 15.9 43 11.5 78 78 0 0 22 0 0 0 0 0 6.40 15.6 41 16.0 75 74 1 0 23 0 2 0 0 0 Months on Test 12 4.20 14.8 40 9.2 65 65 0 0 33 1 1 0 0 0 6.76 16.6 43 12.6 70 70 0 0 28 1 1 0 0 0 3 6.52 16.0 43 11.0 77 75 2 0 22 1 0 0 0 0 104 39 170 2.0 9 6.7 0.76 - 0.1 2.9 170 37 150 5.2 9 6.2 1.11 1.1 0.1 3.3 116 23 180 10.7 19 5.6 1.03 0.6 0.2 2.8 . 112 23 215 10.7 11 5.6 0.87 0.7 0.2 2.6 89 26 215 11.2 13 6.4 0.89 0.8 0.7 3.0 10 L,A,C1 1950 N N N 6.4 0.1 N N 184 0 0 0-1 2+ 0 90 A, Cl 2525 P N N 6.4 0.1 N N 184 0 0 0-1 3+ 0 130 D,A, Cl, P 2460 N 1+ N 6.6 1.0 N Tr 130 0 0-1 0-1 1+ 0 195 D,Y,C1,P 1546 N N N 7.0 1.0 N Tr 112 0 0-1 0 2+ 0 165 L,A,C1,P 2281 N Tr N 6.4 1.0 P Tr 148 0 0-1 0-4 2+ 0 Company Sanitized. Does not contain T S C A CBI Dog No.: 1136 Sex: Female Group ;___ 750 ppm Hematology Erythrocytes Hemoglobin Hematocrit Leucocytes DIFFERENTIAL Neutrophils Seg. Juv. Myel. Lymphocytes Eosinophils Monocytes Basophils Atypicals Nucleated RBC Biochemistry Glucose Urea Nitrogen Cholesterol Aik. Phos. GPT Total Protein A/G . Creatinine Bilirubin Albumin Urinalysis Volume Appearance Osmolality Occult Blood Sugar Acetone PH Urobilinogen Bilirubin Protein Creatinine MICROSCOPIC RBC 's WBC's Epithelial Bacteria Cast DS **SmBUKArORYJ}ATA ON INDIVIDUAL DOGS FED FOR THREE MONTHS Control ' 6.40 17.4 46 17.3 68 68 0 0 27 5 0 0 0 0 6.64 17.7 46 14.0 64 61 3 0 24 10 2 0 0 0 Months on Test 12 7.04 17.4 48 16.9 71 68 3 0 21 8 0 0 0 0 6.48 16.2 42 14.8 63 62 1 0 26 11 0 0 0 0 3 6.88 17.3 46 10.5 49 49 0 0 33 18 0 0 0 0 102 12 120 4.8 5 6.4 1.24 0.5 0.1 3.5 90 12 140 4.7 2 5.9 1.50 0.7 0.2 3.5 94 21 210 6.3 8 6.3 0.78 0.6 0.4 2.8 106 -12 275 7.6 9 6. 6 0.98 0.6 0.1 3.3 109 12 235 7.9 7 6.9 0.63 0.6 0.2 2.7 275 ,Y,C1,P 1677 N N N 7.0 0.1 N Tr 130 0 0 0-1 1+ 0 185 D,Y,C1,P 1388 N N N 8.2 1.0 N Tr 110 0 0 0-1 2+ 0 195 A, Cl, P 1772 N Tr N. 7.0 1.0 N Tr 114 0 0-1 0-1 1+ 0 135 D,Y, Cl, P 1609 N Tr N 8.2 1.0 P Tr 132 0 0 0-2 1+ 0 150 D. Y, Cl, P 1735 N Tr N 7.2 1.0 P Tr 242 0 1-5 0-3 3+ 0 Company Sanitized. Does not contain T S C A CBi Dog No. Sex: Group : 1139 Female 750 ppm Hematology Erythrocytes Hmoglobin Hematocrit Leucocytes DIFFERENTIAL Neutrophils Seg. Juv. Myel. Lymphocytes Eosinophils Monocytes Basophils Atypicals Nucleated RBC Biochemistry Glucose Urea Nitrogen Cholesterol Aik. Phos. GPT Total Protein A/G Creatinine Bilirubin Albumin Urinalysis Volume Appearance Osmolality Occult Blood Sugar Acetone pH Urobilinogen Bilirubin Protein Creatinine MICROSCOPIC RBC's WBC's Epithelial Bacteria Cast CLINICAL L O O R A T O R Y J A T A ON INDIVIDUAL DOGS THREE MONTHS Control 6.08 16.7 43 12.7 66 66 0 0 29 2 3 0 0 0 6.24 17.4 4151 . 8 70 68 2 0 27 1 2 0 0 0 Months on Test 4.40 16.5 44 11.5 71 71 0 0 24 5 0 0 0 0 7.28 18.5 48 16.6 79 78 1 0 21 0 0 0 0 0 6.64 17.1 4150.8 62 62 0 0 30 5 3 0 0 0 104 19 170 6.1 7 6.2 0.97 0.5 0.1 3.1 160 Yj Cl, P 1399 N .N N 7.0 1.0 N Tr 112 0 0-2 0-1 2+ 0 110 13 170 5.9 3 6.2 1.00 0.6 0.1 3.1 98 21 190 129.6 5.8 1.35 0.6 0.3 3.3 120 14 250 10.1 13 6.4 1.16 0.7 0.1 3.4 99 15 340 9.3 9 6.8 0.92 0.6 0.1 3.3 80 Y,C1,P 1210 N N N 7.0 N Tr 128 0 0-2 0 2+ 0 90 Y, Cl 1535 N Tr N 7.0 p Tr 94 75 Y,C1,P 1493 P N N 7.0 U J. P Tr 172 90 Y, Cl 1562 N N N 7.2 l. r T-r 158 0 1-6 o 0-1 0-2 0-2 0-1 0-1 0 -k 1+ 2+ 2+ 0 00 Company sanitized. Does not contain TSCA CBi Dog No. Sex: Group ; 1144 Female 750 ppm Hematology Erythrocytes Hemoglobin Hematocrit Leucocytes DIFFERENTIAL Neutrophils Seg. Juv. Myel. Lymphocytes Eosinophils Monocytes Basophils Atypicals Nucleated RBC Biochemistry Glucose Urea Nitrogen Cholesterol Aik. Phos. GPT Total Protein A/G Creatinine Bilirubin Albumin Urinalysis Volume Appearance Osmolality Occult Blood SugarAcetone pH Urobilinogen Bilirubin Protein Creatinine MICROSCOPIC RBC's WBC's Epithelial Bacteria Cast CLINICAL LABOgATOPynA'lTA on ind i v i d u a l dogs FED OR THREE MONTHS Control 6.40 15.0 38 12.4 80 77 3 0 17 2 1 0 0 0 6. 80 17.3 45 11.0 72 71 1 0 25 3 0 0 0 0 112 18 160 4.0 7 5.9 1.31 0.6 0.2 3.3 115 19 185 3.5 4 6.1 1.32 0.8 0.2 3.5 Months on Test 7.32 18.2 50 11.4 79 79 0 0 20 1 0 0 0 0 80 25 200 4.4 8 6.1 1.25 0.7 0.6 3.4 7.76 19.2 50 13.4 77 75 2 0 22 1 0 o o 0 7.12 18.1 48 12.5 70 67 3 n <?4.Qy 1X ft ft ft 0 104 27 245 6.4 9 6.6 1.10 0.9 0.7 3.5 100 26 285 8.6 9 6.5 1.13 0.8 1.2 3.4 150 D,Y, C1,P 1898 N N N 7.0 1.0 P Tr 184 0 0 0 2+ 0 150 A, Cl, P 1877 N Tr N 7.0 1.0 N Tr 188 0 0 0 2+ 0 135 A, Cl 1766 N ' 1+ N 6. 6 1.0 p Tr 126 0 0 n_i 3+ 0 145 A, Cl 1898 N N N 6.6 1.0 p N 148 0 0 0 1+ 0 195 A, Cl, P 2194 N N N 6.8 1.0 *TD Tr 166 0 0-1 0-3 2+ 0 Does nof contain T$CA C B l Dog No.: 1148 Sex: Female Group ;___ 750 ppm Hematology Erythrocytes Hemoglobin Hematocrit Leucocytes DIFFERENTIAL Neutrophils Seg. Juv. Myel. Lymphocytes Eosinophils Monocytes Basophils Atypicals Nucleated RBC Biochemistry Glucose Urea Nitrogen Cholesterol Aik. Phos. GPT Total Protein A/G Creatinine Bilirubin Albumin Urinalysis Volume Appearance Osmolality Occult Blood Sugar Acetone pH Urobilinogen Bilirubin Protein Creatinine MICROSCOPIC RBC 1s WBC's Epithelial Bacteria Cast CLINICAL IABORATORiyiATA ON INDIVIDUAL DOGS F E D ^ ^ ^ ^ H | FOR THREE MONTHS . Control________ 6.28 15.8 42 9.2 59 58 1 0 41 0 0 0 0 0 6.88 16.5 43 11.8 71 70 1 0 29 0 0 0 0 0 _________ Months on Test______ i 23 7.08 17.0 47 12.9 56 56 0 0 34 8 2 0 0 0 7.28 17.7 46 14.4 51 50 1 0 38 9 2 0 0 0 7.16 17.3 46 11.2 67 66 1 0 28 5 0 0 0 0 106 26 150 3.1 8 5.8 1.11 0.2 3.1 116 25 160 2.9 5 5.9 1.10 0.8 0.4 3.1 . 108 26 235 4.4 12 6.2 1.24 0.7 0.3 3.4 120 22 260 4.6 13 6.3 1.03 0.8 0.3 3.2 112 31 310 3.4 11 7.0 0.94 0.8 0.4 3.4 115 Y, Cl, P 1777 N N N 6.8 1.0 P Tr 174 0 0-1 0-1 2+ 0 110 D,Y, Cl 1856 N N N 6.8 1.0 P N 184 0 0 0 1+ 0 180 D,Y, Cl 1436 N N N 7.0 1.0 P Tr 114 0 0 n\j 3+ 0 33 Y, Cl 392 P N N 7.0 0.1 P Tr 50 0 0-1 0-2 1+ 0 125 D,Y, Cl 2034 N Tr N 7.0 1.0 P Tr 208 0 0-1 0-2 2+ 0 Company Sanitized. Does not contain T S C CBS w m $U lillN JLU A L LABORATORY.D)AATA ON INDIVIDUAL DOGS FED OR THREE MONTHS Dog No. : 1134 Sex: Female Group :___ 2500 ppm Hematology Erythrocytes Hemoglobin Hematocrit Leucocytes DIFFERENTIAL Neutrophils Seg. Juv. Myel. Lymphocytes Eosinophils Monocytes Basophils Atypicals Nucleated RBC Biochemistry Glucose Urea Nitrogen Cholesterol Aik. Phos. . GPT Total Protein A/G Creatinine Bilirubin Albumin Jrinalysis Volume Appearance Osmolality Occult Blood Sugar . Acetone pH Urobilinogen Bilirubin Protein Creatinine MICROSCOPIC RBC 's WBC's Epithelial Bacteria Cast _______ Control 5.68 17.6 44 10.5 57 57 0 0 38 3 2 0 0 0 6. 18. 46 9. 65 65 0 0 30 3 2 0 0 0 112 11 140 4.4 8 6.3 1.37 0.5 0.1 3.6 100 9 155 6.8 3 6.2 1.1 0.6 0.2 3.3 120 D,Y,C1,P 2116 N Tr N 7.0 1.0 N Tr 156 0 0-1 0 2+ 0 130 D,Y,C1,P 1798 N Tr N 7.0 1.0 N Tr 124 0 0 0-1 1+ 0 Months on Test 12 3 6.40 16.8 45 9.0 67 66 1 0 27 3 3 0 0 0 6.60 17.1 44 13.2 75 73 2 0 19 4 2 0 0 0 6. 60 16.8 44 9.1 68 68 0 0 29 3 0 0 `0 0 91 13 235 15.4 14 6.5 0.94 0.6 0.3 3.1 110 16 290 17.7 24 6.7 0.97 0.8 0.2 3.3 124 17 285 15.2 17 6.8 1.05 0.8 0.5 3.5 165 A, Cl, P 1724 N Tr * N 6.8 1.0 N N 116 0 0-1 0 3+ 0 175 D,Y,C1,P 1436 N N N 7.0 1.0 P N 100 0 0 0 3+ 0 170 A, Cl, P 1903 N N N 7.2 1.0 P 1+ 144 0 0 0-1 3+ 0 Company Sanitized. Dog No.: 1138 Sex: Female Group :___ 2500 ppm Hematology Erythrocytes Hemoglobin Hematocrit Leucocytes DIFFERENTIAL Neutrophils Seg. Juv. Myel. Lymphocytes Eosinophils Monocytes Basophils Atypicals Nucleated RBC Biochemistry Glucose ' Urea Nitrogen Cholesterol Aik. Phos. GPT Total Protein A/G . Creatinine Bilirubin Albumin Urinalysis Volume Appearance Osmolality Occult Blood Sugar Acetone PH Urobilinogen Bilirubin Protein Creatinine MICROSCOPIC RBC 's WBC 's Epithelial Bacteria Cast )ATA ON INDIVIDUAL DOGS FE OR THREE MONTHS Control - 6.52 17.1 44 8.5 73 72 1 0 22 3 2 0 0 0 6. 68 17.4 45 7.7 69 69 0 0 20 8 3 0 0 0 Months on Test 12 5.84 14.9 39 10.7 70 70 0 0 28 2 0 0 0 0 5.16 12.8 31 9.7 77 75 2 0 23 0 0 0 0 0 3 6.16 15.3 41 7.4 63 60 3 0 34 1 2 0 0 0 104 14 175 8.0 6 6.5 1.09 0.4 0.1 3.4 102 11 180 7.6 3 6.3 0.97 0.6 0.2 3.1 90 21 348 12.8 11 6.6 0.88 - 0.6 0.3 3.1 120 ~~ 17 436 14.1 16 6.7 0.78 0.7 0.2 2.9 108 16 275 13.5 17 6.3 1.28 0.8 0.2 3.5 270 Y, Cl, P 1399 N N N 7.0 0.1 N Tr 104 0 0-1 0-1 2+ 0 380 Y, Cl,P 931 N N N 7.0 0.1 N N 72 0 0-1 0-1 4+ 0 415 Y, Cl 684 N N N. 6.8 0.1 N N 48 0 0 0-1 3+ 0 290 Y, Cl, P 862 N N N 7.0 1.0 P N 82 0 0-1 0 4+ 0 175 A,Cl,P 1420 N N N 7.2 1.0 N Tr 158 ' 0 0 0-1 2+ 0 Company Sanitized Does not eontain T S C C3 x<xixlSXOl.Xi AX& ON INDIVIDUAL DOGS "o r THREE MONTHS Dog No.: 1142 Sex: Female Group:___ 2500 ppm Hematology Erythrocytes Hemoglobin Hematocrit Leucocytes DIFFERENTIAL Neutrophils Seg. Juv. Myel. Ly hocytes Eosinophils Monocytes Basophils Atypicals Nucleated RBC Biochemistry Glucose Urea Nitrogen Cholesterol Aik. Phos. GPT Total Protein A/G Creatinine Bilirubin Albumin Urinalysis Volume Appearance Osmolality Occult Blood Sugar Acetone PH Urobilinogen Bilirubin Protein Creatinine MICROSCOPIC RBC 's WBC 's Epithelial Bacteria Cast ________Control ~' 5.76 16.1 41 16.1 41 40 1 0 23 36 0 0 0 0 6.00 16.3 41 12.3 65 65 0 0 28 7 0 0 0 0 110 29 140 3.5 8 6.4 1.11 0.6 0.6 3.4 120 2Q. 155 3.8 3 6.6 1.02 0.8 0.2 3.3 240 D,Y, Cl, P 1766 N .N N 6.8 0.1 N N 108 0 0 0 1+ 0 175 L,A, Cl, P 2272 N Tr N 6.8 1.0 N Tr 144 0 0 0-3 1+ 0 Months on Test 12 5.28 13.6 36 13.3 54 52 2 0 30 16 0 0 0 0 5.96 15.9 40 14.7 66 66 0 0 27 7 0 0 0 0 3 5.72 14.9 39 8.5 61 61 0 0 25 13 1 0 0 0 92 23 265 . 6.1 16 6.1 1.02 0.7 0.2 3.1 108 28 260 9.2 16 6.6 0.89 0.8 0.5 3.1 102 21 235 8.6 15 6.3 0.86 1.0 0.2 2.9 0 1 f--1 115 A, Cl, P 2168 N Tr N 7.0 1.0 N Tr 164 0 0-1 2+ 0 165 A, Cl, P 2355 N -N N 6. 6 1.0 N Tr 158 c "d *rl 40J) uQJ d 4J Gri d Sa PQ GU J- O -H u& 165 A, Cl, P 2168 N N N 7.0 1.0 N Tr 178 0 0 0 2+ 0 Company Sanitized. Does not contain TSOAGtai Dog No.: 1146 Sex: Female Group :___ 2500 ppm tiematoiogy Erythrocytes Hemoglobin Hematocrit Leucocytes DIFFERENTIAL Neutrophils Seg. Juv. Myel. Lymphocytes Eosinophils Monocytes Basophils Atypicals Nucleated RBC Biochemistry Glucose Urea Nitrogen Cholesterol Aik. Phos. GPT Total Protein A/G Creatinine Bilirubin Albumin Urinalysis Volume Appearance Osmolality Occult Blood Sugar Acetone pH Urobilinogen Bilirubin Protein Creatinine MICROSCOPIC RBC'S WBC's Epithelial Bacteria Cast wwj.j.ixunu jj. urvriuj\l iA UJN 1HJJ1VID UAL DUGS FED FOR THREE MONTHS Control 6.48 15.4 40 11.1 67 66 1 0 31 0 2 0 0 0 6.72 16.7 44 8.6 64 64 0 0 35 1 0 0 0 0 Months on Test 1 23 ---------------------------------- - 5.88 14.3 38 11.4 6.00 14.2 36 12.3 5.88 15.0 39 12.6 61 65 62 61 65 62 0 00 0 00 31 34 30 5 06 3 12 0 0o 0 0o 0 00 97 18 155 3.8 6 5.8 1.17 0.6 0.1 3.1 108 15 175 4.0 3 6.0 1.41 0.7 0.1 3.5 110 26 260 7.8 11 6.3 1.07 0.6 0.2 3.3 116 23 270 8.3 17 6.2 1.06 0.8 0.1 3.2 112 19 230 8.9 12 6.6 1.28 0. 9 0.1 3.7 200 Y,C1,P 1189 N N N 7.2 0.1 N Tr 108 0 0 0-1 2+ 0 110 Y,C1 743 N N N 7.0 0.1 N Tr 136 0 0-2 0-1 3+ 0 140 Y, Cl, P 1247 N .N N 7.0 0.1 N Tr 96 0 0-1 0-1 4+ 0 80 D, Y, Cl 1724 N N N 7.2 1.0 N Tr 158 0 0 0-2 2+ 0 95 D,A,C1,P 1942 N Tr N 8.0 1.0 P 1+ 198 0 0 0-1 2+ 0 Company Sanitized. Does not contain T S C A CBt