Document LJDJQxZJYrJx2wQxeE5OXyoZz

3M General Offices U&S1 f f 3M May 4, 2000 3M Center St. Paul, MN 55144-1000 612 733 1110 FYI-00-001378 F Y J- OS- \blB /)12-004H FY I- 0 0 - 0 0 137B 85000000006 8500000000E ,CJ C -t TT ro cn "t - o et VIA FEDERAL EXPRESS *7 Dr. Charles Auer Director Chemical Control Division Office Of Pollution Prevention And Toxics United States Environmental Protection Agency 401 M Street, Southwest Room 403 East Tower (Mail Code 7405) Washington, D. C. 20460 Re: Information On Perfluorooctane Sulfonates Dear Charlie: Pursuant to our recent communications, 3M is enclosing additional information on perfluorooctane sulfonates. The enclosed information supplements information submitted to you previously under cover of our April 21, 2000 letter. Again, we are providing this information on a voluntary basis as part of our continuing discussions with EPA regarding fluorochemistry. The enclosed information covers perfluorooctane sulfonates, including CAS numbers 1763-23-1 (acid); 29081-56-9 (ammonium salt); 70225-14-8 (DEAsalt); 2795-39-3 (potassium-salt); 29457-72-5 (lithium salt). It consists of the following: => Copies of post-1975 studies and certain other information relating to the following environmental science areas: (i) physical and chemical properties; (ii) environmental fate and transport; (iii) environmental monitoring; and (iv) ecotoxicity. For each study, 3M has prepared a summary in the HPV "robust summary" format. An executive summary also has been included for each area. => Copies of post-1975 studies and certain other information relating to the following health effects areas: (i) acute toxicity; (ii) genotoxicity; (iii) repeated-dose toxicity; OOOOOl Charles Auer May 4, 2000 Page 2 _ -nr. (iv) pharmacokinetics, (v) teratology; and (vi) medical surveillance and epidemiology. 3M has included a detailed index of this information. => A list of all studies in progress and planned studies, along with study protocols or study plans, where available. With regard to the health effects area, this list supplements the list provided under cover of our April 21, 2000 letter to you. => A bibliography of pre-1976 studies in the environmental science and health effects areas on perfluorooctane sulfonates. => A bibliography of acute toxicity studies on perfluorooctane sulfonates, except that we are providing copies of key acute studies (with reference to the HPV guidance). => A bibliography of published studies on the perfluorooctane sulfonates in 3M's possession. => An index of submissions made by 3M to the TSCA Section 8(e) docket. This index has been subdivided by EPA docket number. Rather than attempt to segment the index for perfluorooctane sulfonates only, we have included other fluorochemical submissions on the index, as several of 3M's submissions have dealt with multiple fluorochemicals. 3M is continuing our file review and will supplement the enclosed information as appropriate. As you review this information, we ask that you bear several points in mind: ==> The enclosed information spans several boxes. W e have organized the information in each box with labeled file folders and indices to aid EPA's review. To ensure that you and your staff are able to access the most pertinent information, we also are attaching to this cover letter the executive summaries for the environmental science areas and the indices covering studies and other information. => In some cases, the enclosed information reflects recent developments that may supplement studies and other information previously provided to you. As just one example, 3M's previously submitted document entitled "Fluorochemical Use, Distribution And Release Overview" (5/26/99) contains a qualitative assessment based on the assumption that all other fluorochemicals could breakdown to perfluorooctane sulfonates. Another document submitted by 3M entitled "Sulfonated Perfluorochemicals in the Environment: Sources, Dispersion, Fate and Effects" also provided estimates of potential exposure and waste generation based on such an assumption. Recent information in the environmental fate and transport area suggests, however, that this assumption may reflect an unrealistic 000002 Charles Auer May 4, 2000 Page 3 "worst case" which significantly overstates exposure potential to perfluorooctane sulfonates. In particular, this information (which is enclosed) indicates that perfluorooctane sulfonates may not be a degradation product of many fluorochemicals and that high molecular weight fluorochemical polymers and fluorochemical phosphate esters are relatively stable in the environment. => The enclosed information includes some studies and other information on mixtures containing perfluorooctane sulfonates. As we have discussed, 3M will be providing you with further information on other fluorochemicals within the next several weeks. W e will include additional studies and other information on mixtures containing perfluorooctane sulfonates at that time. => 3M has not provided you with all analytical chemistry reports on perfluorooctane sulfonates. Rather, we have enclosed certain analytical chemistry reports which may prove useful to EPA in interpreting certain studies; understanding the details of analytical chemistry methods; or verifying human and biomonitoring data. => 3M is continuing its work to refine the analytical characterization of the perfluorooctane sulfonates test material being used for our current study program. We will keep you informed of any pertinent developments. => Finally, please note that some of this information qualifies as confidential business information (CBI); CBI information has been placed in a separate, labeled envelope. Also, incorrectly applied legends relating to legal privileges and proprietary protections have been removed from certain documents. 000003 Charles Auer May 4, 2000 Page 4 3M looks forward to discussing the enclosed information with you and other EPA staff. In the meantime, please do not hesitate to contact me with any questions. Very truly yours, Enclosures William A. Weppner, Ph.D Director of Environmental, Health, Safety And Regulatory Affairs Specialty Materials Markets 3M Bldg 236-1B-10 3M Center St. Paul, MN 55144 E-mail: waweppner(a).mmm.com 000004 Attachment to Letter to C. Auer Dated May 4, 2000: Environmental Studies on Perfluorooctanesulfonates (Post-1975) Physical/chemical Properties Title Determination of the Melting Point/Melting Range of PFOS; Boiling Point (Not Conducted) Laboratory or Author Wldlife International, Ltd. Determination of the Vapor Pressure of PFOS Using the Spinning Wildlife Rotor Guage Method International, Ltd. PFOS: Determination of the n-Octanol/Water Partition Coefficient Wildlife by the Shake Flask Method - A Non-GLP Feasibility Study in International, Ltd. Support of Wildlife International, Ltd. Project Number: 454C-108 Completion Date 2/24/99 Type Robust Summaries, Final Report, Protocol 5/5/99 Robust Summary, Final Report. Protocol 2/11/00 Robust Summary, Feasibility Study Testing Results: Air-Water Partition Coefficient (KAW) for PFOS 3M/Wldlife International, Ltd., U of Trent Determination of the Water Solubility of PFOS by the Shake Flask Wildlife Method International, Ltd. Technical Report. Solubility Measurements on FC-95 3M Env. Lab Solubility Estimate of FC-95 by use of Xertex TOC Analyzer Xertex, 3M Env. Lab 3/19/99 4/26/00 2/6/81 6/29/82 Robust Summary, Letter Report Robust Summary, Final Report, Protocol Brief Robust Summary, critique from Endwin Tucker (3/1/93), Final Report Brief robust summary, letter report Environmental Fate and Transport Title Adsorption of FC 95 and FC 143 on Soil (Note: the 3M Env. Lab summary is titled: Summary of the Soil Adsorption study of the Potassium Salt of Perfluorooctanesulfonic acid, 7/22/98) FC-95/Photolysis Study Using Simulated Sunlight. (Note: the 3M Env. Lab summary is titled: summary of Photolysis Study Using Simulated Sunlight on the Potassium Salt of Perfluorooctanesulfonic acid) Biodegradation Studies of Fluorocarbons (8/12/76) report and Biodegradation Studies of Fluorocarbons - III (7/19/78) report. (Note: both reports summarized with one robust summary) BOD/COD results for FC-94-X (Li salt of PFOS) BOD/COD results for FC-99 (DEA salt of PFOS) Transport between environmental compartments (fugacity modeling) included in letter from Don Mackay on the air/water partitioning coefficient calculations Analysis for fluorochemicals in Bluegill fish. Laboratory or Author 3M Env. Lab 3M Env. Lab 3M Env. Lab Pace Analytical 3M Env. Lab DMER 3M Env. Lab Completion Date 2/27/78 Type Robust Summary, 3M Env. Lab Summary, Comments from Stephen A. Boyd from MSU, Final Report 1/9/79 Robust Summary, 3M Env. Lab Summary, Final Report 8/12/1976, 7/19/78 Brief Robust Summary, 2 Final Reports 3/30/94 6/8/79 Computer-generated Summary of Testing Results Robust Summary and final Reports No date Robust Summary, letter report 5/17/79 Robust Summary, Technical Report 000005 Ecotoxicity Elements Title PFOS: A 96-Hour Static Acute Toxicity Test with the Fathead Minnow (P im eph ales p ro m e ta s ) Laboratory or Author Wildlife International, Ltd. Completion Date 4/26/00 Type Robust Summary, Final Report, Protocol PFOS: A 96-Hour Toxicity Test with the Freshwater Alga (Selenastrum capricomutum ) Wildlife International, Ltd. 4/26/00 Robust Summary, Final Report, Protocol PFOS: A 48-Hour Static Acute Toxicity Test with the Cladoceran Wildlife (Daphnia m agna) International, Ltd. 4/26/00 Robust Summary, Final Report, Protocol PFOS: A 96-Hour Shell Deposition Test with the Eastern Oyster (Crassostrea virginica) Wildlife International, Ltd. 4/26/00 Robust Summary, Final Report, Protocol PFOS: A 96-Hour Static Acute Toxicity Test with the Freshwater Wildlife Mussel (Unto com ptam atus) International, Ltd. 4/26/00 Robust Summary, Final Report, Protocol PFOS: An Activated Sludge, Respiration Inhibition Test Wildlife International, Ltd. 4/28/00 Robust Summary, Final Report, Protocol PFOS: A 96-Hour Static Acute Toxicity Test with the Saltwater Mysid (M ysidopsis b ah ia) Wildlife International, Ltd. 4/26/00 Robust Summary, Final Report, Protocol PFOS: An Early Life-Stage Toxicity Test with the Fathead Minnow Wildlife (iP im eph ales pro m etas) International, Ltd. 4/26/00 Robust Summary, Final Report, Protocol PFOS: A Semi-Static Life-Cycle Toxicity Test with the Cladoceran Wildlife ((Daphnia m agna) International, Ltd. 4/26/00 Robust Summary, Final Report, Protocol PFOS: A Flow-through Life-Cycle Toxicity Test with the Saltwater Wildlife Mysid (M ysidopsis b ah ia) International, Ltd. 4/26/00 Robust Summary, Final Report, Protocol PFOS: A Dietary LC50 Study with the Mallard Wildlife International, Ltd. 4/26/00 Robust Summary, Final Report, Protocol PFOS: A Dietary LC50 Study with the Northern Bobwhite Wildlife International, Ltd. 4/26/00 Robust Summary, Final Report, Protocol Multi-Phase Exposure/Recovery Algal Assay Test Method 3M Env. Lab The Effects of Continuous Aqueous Exposure to 14C-78.02 on Hatchability of Eggs and Growth and Survival of Fry of Fathead Minnow (Pim ephales p ro m etas) and Summary of histopathological Examinations of Fathead Minnow (Pimephales promelas) Exposed to 78.02 for 30 Days EG&G Bionomics 12/16/81 Brief Robust Summary, Final Report August, 1978 Brief Robust December, Summary, Final Reports 1978 Effect of Potassium Perfluorooctanesulfonate on Survival, etc. (Daphnid reproduction) Pimephales promelas 96-hour Toxicity Test Data Summary. Sample FC-94-X (Li salt of PFOS) 48-HR Acute Toxicity to Daphnia, D aphnia m ag n a. FC-94-X (Li salt of PFOS) Microbios Microtox Toxicity Test. Sample: FC-94-X (Li salt of PFOS) Evaluation of FC-94-X by OECD Activated Sludge Respiration Inhibition Test #209, Review of OECD 209 and BOD/COD Test Results for FC-94-X, test data sheets (Li salt of PFOS) 96-Hour Acute Toxicity Test on Bluegill Sunfish (FC-99, DEA salt of PFOS) Acute Toxicity to Activated Sludge (FC-99, DEA salt of PFOS) Microtox data for FM-3820 (28% PFOS) 3M Env. Lab 3M Env. Lab 3M Env. Lab 3M Env. Lab 3M Env. Lab Analytical Biochemistry Laboratories, Inc. 3M Env. Lab 3M Env. Lab 2/13/84 3/25/94 2/10/94 2/7/94 7/22/98 7/13/79 Brief Robust Summary, Final Report Robust Summary, Summary Page, copies of data Robust Summary, copies of data Robust Summary, summary of results, copies of data. Robust Summary, Summary Page, copies of data Robust Summary and Final Report 6/4/79 7/24/91 Robust Summary and copies of data Robust Summary and data summary Acute Toxicity to Daphnia magna for FM-3820 (28% PFOS) Toxicity to Algae (Selenastrum capricomutum) for FM-3820 (28% PFOS) EnviroSystems of Resource Analysts, Inc.) EnviroSystems of Resource Analysts, Inc.) 3/26/91 Robust Summary and Final Report June, 1991 Robust Summary and Final Report Summary Reports Title Final Comprehensive Report: FC 95 Laboratory or Author 3M Env. Lab Completion Type Date 3/15/79 Robust Summary, comments from Stephen A. Boyd from MSU and Final Report Environmental Monitoring P a rti: Multi-City Study Title Design and Structure of Multi-City Study Low Level Drinking Water Analytical Method Fluorochemical Characterization of Drinking Water Samples. Columbus, GA (W2336) Fluorochemical Characterization of Drinking Water Samples. Pensacola, FL(W2176) Fluorochemical Characterization of Drinking Water Samples. Port St. Lucie, FL (W2363) Fluorochemical Characterization of Drinking Water Samples. Decatur, Alabama (W1979) Fluorochemical Characterization of Drinking Water Samples. Mobile, Alabama (W2151) Fluorochemical Characterization of Drinking Water Samples. Cleveland, Tennessee (W1973) Draft Drinking Water Health Advisory (DWHA) - PFOS Battelle Field Sampling Procedures Review. Columbus Georgia City Survey regarding Empirical Human Exposure Assessment. Multi-City Study Multi-City Study. Field Report for Cleveland Tennessee and Decatur Alabama - Battelle Duxbury Activities Multi-City Study. Field Report for Columbus Georgia and Port St. Lucie Florida - Battelle Duxbury Activities Final Multi-City Study. Field Report for Mobile Alabama and Pensacola Florida - Battelle Duxbury Activities Quality assurance Project Plan for Empirical Human Exposure Assessment. Multi-City Study Sampling Task Amendment 1 to the Quality Assurance Project Plan and Associated SOP's Laboratory or Author Battelle Memorial Institute 3M Env. Lab Centre Analytical Laboratories, Inc. Centre Analytical Laboratories, Inc. Centre Analytical Laboratories, Inc. Centre Analytical Laboratories, Inc. Centre Analytical Laboratories, Inc. Centre Analytical Laboratories, Inc. 3M Corporate Toxicology 3M Env. Lab Battelle Memorial Institute Battelle Memorial Institute Battelle Memorial Institute Battelle Memorial Institute Battelle Memorial Institute Completion Type Date 5/1/00 Report 4/28/00 2/29/00 Method Final Report 2/28/00 Final Report 2/28/00 Final Report 2/28/00 Final Report 2/28/00 Final Report 2/28/00 Final Report 7/7/99 Report 8/3/99 Final Report 7/9/99 Final Report 10/26/99 Final Report 9/29/99 Final Report 5/14/99 QAP 6/16/99 Ammendment 000007 Part 2: Biosphere Studies Title Laboratory or Author LCMSMS Analysis of Extracts Reported in: "Preliminary Report Michigan State Analysis of Perfluorinated Compounds in Environmental Samples" University (P. Jones and K. Kannan) Completion Date 4/7/99 Typ Report Analysis of Fluorochemicals in Wild Bird Livers Screening of PROS levels in Eagle and Albatross 3M Env. Lab 3M Env. Lab 4/28/99 5/8/98 Final Report Report 000008 ATTACHMENT TO LETTER TO C. AUER DATED MAY 4,2000: PRE-1976 ENVIRONMENTAL STUDIES ON PERFLUOROOCTANESULFONATES Data from Fathead Minnow Study on FC-93 (25% NH4 salt of PFOS in IPA and water), 3M Environmental Laboratory, Aug. 2, 1974. (Robust summary, MSDS and copies of data being provided to EPA) Data from Fathead Minnow Study on FC-93 (25% NH4 salt of PFOS in IPA and water), 3M Environmental Laboratory, Oct. 19, 1974. (Robust summary, MSDS and copies of data being provided to EPA) BOD/COD results for FC-93 (25% NH4 salt of PFOS in IPA and water), 3M Environmental Laboratory. Completion date: July 18, 1974. (Robust summary and copy of results being provided to EPA) 000009 ATTACHMENT TO LETTER TO C. AUER DATED MAY 4,2000: ONGOING ENVIRONMENTAL STUDIES ON PERFLUOROOCTANESULFONATES Physical/Chemical Properties Potential Fluorochemical Combustion By-Products (involves review of results of literature search regarding potential for formation of florindated dioxins and furans), 3M Environmental Laboratory. Expected completion: Sept. 2000. Study paper in progress. Fluorochemical Decomposition Process: Quantification and Assessment (involves computational chemistry calculations of bond-breaking strengths of sulfonated perfluorochemicals), Battelle Memorial Institute. Expected completion: Aug. 2000. Study paper in progress. Environmental Fate and Transport Abiotic Degradation Studies (hydrolysis and indirect photolysis), 3M Environmental Laboratory. I Expected completion: June 2000 (hydrolysis); Aug. 2000 (indirect photolysis). (Summary study plan and screening results summary being provided to EPA) Biodegradation Studies (aerobic acclimated closed bottle biodegradation, aerobic soil/sediment I biodegradation, pure culture aerobic, and fluorochemical decomposition process, stability in ( .X water, photodegradation), Springbom Laboratories, Inc. Expected completion: Aug. 2000. (Summary study plan being provided to EPA) fj anv - - J M /Lj fa ry w fiJ Ecotoxicity Elements * / ^ PFOS: A 96-Hour Toxicity Test with the Freshwater Alga (Anabaenaflos-aquae), Wildlife International, Ltd. Expected completion: July 2000. (Protocol being provided to EPA) PFOS: A 96-Hour Toxicity Test with the Freshwater Diatom (Naviculapelliculosa), Wildlife International, Ltd. Expected completion: July 2000. (Protocol being provided to EPA) _ PFOS: A 96-Hour Toxicity Test with the Marine Diatom (Skeletonema costatum), Wildlife International, Ltd. Expected completion: July 2000. (Protocol being provided to EPA) PFOS: A 7-Day Toxicity Test with Duckweed (Lemna gibba), Wildlife International, Ltd. Expected completion: July 2000. (Protocol being provided to EPA) Phytotoxicity - Seedling Emergence, Wildlife International, Ltd. Expected completion: July 2000. Protocol in progress. Environmental Monitoring ft Global Environmental Sampling Plan, Michigan State University. Expected completion: Dec. 2000. (Summary being provided to EPA) O O O O IO Haft ^V"GS f * G-Sl# >*tl P A i/U l Af Attachments to Letter to C. Auer dated May 4, 2000 Perfluoroctane Sulfonate Studies Acute Toxicity 1) An Acute Inhalation Toxicity Study of T-2306 CoC in the Rat, Bio/dynamics, Inc., Project No. 78-7185, December 31, 1979. (FC-95, Perfluorooctane Sulfonate potassium salt) 2) Acute Oral Toxicity (LD50) Study in Rats with Fluorad Fluorochemical Surfactant FC-95, International Research and Development Corporation, Project No. 137-083, May 31, 1978. (Note: 3M files indicate samples taken during the study were not analyzed.) 3) Eye and Skin Irritation Studies Report on Sample T-1166 (FC-98, Potassium Perfluoroethylcyclohexyl Sulfonate, presumed 100 %), Warf Institute Inc., Project No. 5011023, January 28, 1975. a) Combined Eye and Skin Irritation Studies Report b) Eye Irritation Study (with washout procedure) Report OOOOll Attachments to Letter to C. Auer dated May 4, 2000 Perfluoroctane Sulfonate Studies Bibliography of Acute Toxicity Studies Not Submitted 1) Acute Oral Toxicity-Rats on Sample T-1389, Biosearch Inc., March 4, 1976 (FC95, Perfluorooctane Sulfonate potassium salt). 2) Acute Oral Toxicity-Rats on Sample T-1390, Biosearch Inc., March 4, 1976. [FC98] 3) Acute Oral Toxicity-Rats on Sample T-2297 CoC (Ammonium Perfluorooctane Sulfonate, FC-93 Solids), Biosearch, Inc. October 13, 1978. 4) Acute Oral Toxicity-Rats on Sample T-2275 CoC (FC-99 Old Formula, L-4299, NB 48490, which is 50 % of the Diethanolamine Salt of Perfluorooctane Sulfonate in water), Biosearch, Inc., September 25, 1978. 5) Primary Skin Irritation Test with T-2509CoC in Albino Rabbits, Safety Evaluation Laboratory, Riker Laboratories, Inc., Experiment No. 479EB0332, 3M Reference no. T-2509.4 (FC-99 New Formula, L-4509, 25 % Diethanolamine Salt of Perfluorooctane Sulfonate in water), June 28, 1979. 6) Acute Ocular Irritation Study with T-2509CoC in Albino Rabbits, Safety Evaluation Laboratory, Riker Laboratories, Inc., Experiment No. 479EB0333, 3M Reference no. T-2509.1 (FC-99 New Formula, L-4509, 25 % Diethanolamine Salt of Perfluorooctane Sulfonate in water), July 13, 1979. 7) Acute Inhalation Toxicity Study of T-2308 CoC in the Rat, Bio/dynamics, Inc., Project No. 78-7187, 3M Reference No. T-2308 (FC-98, Potassium Perfluoroethylcyclohexyl Sulfonate, presumed 100 %), April 12, 1979. 8) Acute Ocular Irritation Test with T-2960CoC in Albino Rabbits, Safety Evaluation Laboratory, Riker Laboratories, Inc., Experiment No. 0880EB0598, 3M Reference No. T-2960.2 (FC-90, L-4649, Diethanolamine Salt of Perfluoroethylcyclohexyl Sulfonate, 25 % in water), February 18, 1981. 9) Acute Dermal Toxicity Study with T-2960CoC in Albino Rabbits, Safety Evaluation Laboratory, Riker Laboratories, Inc., Experiment No. 0880AB0599, 3M Reference No. T-2960.1 (FC-90, L-4649, Diethanolamine Salt of Perfluoroethylcyclohexyl Sulfonate, 25 % in water), January 15, 1981. 10) Primary Skin Irritation Test with T-2960CoC in Albino Rabbits, Safety Evaluation Laboratory, Riker Laboratories, Inc., Experiment No. 0880EB0597, 3M Reference 000012 Attachments to Letter to C. Auer dated May 4, 2000 Perfluoroctane Sulfonate Studies No. T-2960.4 (FC-90, L-4649, Diethanolamine Salt of Perfluoroethylcyclohexyl Sulfonate, 25 % in water), January 15, 1981. 11) Acute Oral Toxicity Study with T-2960CoC in Albino Rabbits, Safety Evaluation Laboratory, Riker Laboratories, Inc., Experiment No. 0980AR0600, 3M Reference No. T-2960.3 (FC-90, L-4649, Diethanolamine Salt of Perfluoroethylcyclohexyl Sulfonate, 25 % in water), February 18, 1981. 12) Acute Oral Toxicity - Rats, Biosearch, Inc., 3M Reference No. T-2296 (FC-93, Ammonium Pefluorooctane Sulfonate, 25 % in 20 % Isopropyl Alcohol and 55 % Water), October 19, 1978. 000013 Attachments to Letter to C. Auer dated May 4, 2000 Perfluoroctane Sulfonate Studies Bibliography of Acute Toxicity Studies Not Submitted 1) Acute Oral Toxicity-Rats on Sample T-1389, Biosearch Inc., March 4, 1976 (FC95, Perfluorooctane Sulfonate potassium salt). 2) Acute Oral Toxicity-Rats on Sample T-1390, Biosearch Inc., March 4, 1976. [FC98] 3) Acute Oral Toxicity-Rats on Sample T-2297 CoC (Ammonium Perfluorooctane Sulfonate, FC-93 Solids), Biosearch Inc. October 13, 1978. 4) Acute Oral Toxicity-Rats on Sample T-2275 CoC (FC-99 Old Formula, L-4299, NB 48490, which is 50 % of the Diethanolamine Salt of Perfluorooctane Sulfonate in water), Biosearch, Inc., September 25, 1978. 5) Primary Skin Irritation Test with T-2509CoC in Albino Rabbits, Safety Evaluation Laboratory, Riker Laboratories, Inc., Experiment No. 479EB0332, 3M Reference no. T-2509.4 (FC-99 New Formula, L-4509, 25 % Diethanolamine Salt of Perfluorooctane Sulfonate in water), June 28, 1979. 6) Acute Ocular Irritation Study with T-2509CoC in Albino Rabbits, Safety Evaluation Laboratory, Riker Laboratories, Inc., Experiment No. 479EB0333, 3M Reference no. T-2509.1 (FC-99 New Formula, L-4509, 25 % Diethanolamine Salt of Perfluorooctane Sulfonate in water), July 13, 1979. 7) Acute Inhalation Toxicity Study of T-2308 CoC in the Rat, Bio/dynamics, Inc., Project No. 78-7187, 3M Reference No. T-2308 (FC-98, Potassium Perfluoroethylcyclohexyl Sulfonate, presumed 100 %), April 12, 1979. 8) Acute Ocular Irritation Test with T-2960CoC in Albino Rabbits, Safety Evaluation Laboratory, Riker Laboratories, Inc., Experiment No. 0880EB0598, 3M Reference No. T-2960.2 (FC-90, L-4649, Diethanolamine Salt of Perfluoroethylcyclohexyl Sulfonate, 25 % in water), February 18, 1981. 9) Acute Dermal Toxicity Study with T-2960CoC in Albino Rabbits, Safety Evaluation Laboratory, Riker Laboratories, Inc., Experiment No. 0880AB0599, 3M Reference No. T-2960.1 (FC-90, L-4649, Diethanolamine Salt of Perfluoroethylcyclohexyl Sulfonate, 25 % in water), January 15, 1981. 10) Primary Skin Irritation Test with T-2960CoC in Albino Rabbits, Safety Evaluation Laboratory, Riker Laboratories, Inc., Experiment No. 0880EB0597, 3M Reference 000014 Attachments to Letter to C. Auer dated May 4, 2000 Perfluoroctane Sulfonate Studies No. T-2960.4 (FC-90, L-4649, Diethanolamine Salt of Perfluoroethylcyclohexyl Sulfonate, 25 % in water), January 15, 1981. 11) Acute Oral Toxicity Study with T-2960CoC in Albino Rabbits, Safety Evaluation Laboratory, Riker Laboratories, Inc., Experiment No. 0980AR0600, 3M Reference No. T-2960.3 (FC-90, L-4649, Diethanolamine Salt of Perfluoroethylcyclohexyl Sulfonate, 25 % in water), February 18, 1981. 12) Acute Oral Toxicity - Rats, Biosearch, Inc., 3M Reference No. T-2296 (FC-93, Ammonium Pefluorooctane Sulfonate, 25 % in 20 % Isopropyl Alcohol and 55 % Water), October 19, 1978. 000015 Attachments to Letter to C. Auer dated May 4, 2000 Perfluoroctane Sulfonate Studies Repeated-Dose Toxicity 1) Ninety-Day Subacute Rhesus Monkey Toxicity Study, with Fluorad Fluorochemical Surfactant FC-95, International Research and Development Corporation, Project No. 137-092, December 18, 1978. a) Study Report b) Aborted Study: Ninety-Day Subacute Rhesus Monkey Toxicity Study, with Fluorad Fluorochemical Surfactant FC-95, International Research and Development Corporation, Project No. 137-087, January 2, 1979. 2) Ninety-Day Subacute Rat Toxicity Study, with Fluorad Fluorochemical Surfactant FC-95, International Research and Development Corporation, Project No. 137-085, November, 1978. 3) 104-week Dietary Chronic Study and Carcinogenicity Study with Perfluorooctane Sulfonic Acid Potassium Salt (PFOS: T-6295) in Rats, Covance Laboratories Inc., Study Number 6329-183. In progress. a) Summary Report - Week 53 undated b) "Liver Slide Review," Marvin Case to John Butenhoff and Andrew Seacat dated April 5, 2000 relaying the results of an independent histopathologic review of liver slides from the study. c) Second Draft Cell Proliferation Report, Pathology Associates International, August 24, 1999. [final interim report, to be incorporated in final report] d) Study Report of Determination of Cyanide Insensitive Palmitoyl-CoA oxidation in samples from 3M Environmental Laboratory - Covance Studies 6329-183 and 6329-212, Centre For Xenobiotic Research, University of Dundee, Biomedical Research Center, Study Number XR0108, February 18, 1999. 4) Range-finder: 4-Week Capsule Toxicity Study with Perfluorooctane Sulfonic Acid Potassium Salt (PFOS; T-6295) in Cynomolgus Monkeys, Covance Laboratories Inc., Study Number 6329-222 a) Unaudited Draft Final Report, 4-Week Capsule Toxicity Study with Perfluorooctane Sulfonic Acid Potassium Salt (PFOS; T-6295) in Cynomolgus Monkeys, Covance Laboratories Inc., Study Number 6329-222 (draft not complete). 000016 Attachments to Letter to C. Auer dated May 4, 2000 Perfluoroctane Sulfonate Studies b) Cell Proliferation Report, 4-Week Capsule Toxicity Study with Perfluorooctane Sulfonic Acid Potassium Salt (PFOS; T-6295) in Cynomolgus Monkeys, Covance Laboratories Inc., Study Number 6329-222 (draft to be incorporated in final report) c) Protocol - Analytical Study, Quantitative Analysis of Perfluorooctane Sulfonic Acid Potassium Salt (PFOS; T-6295) in Cynomolgus Monkeys Following Administration of a 4-Week Capsule Toxicity Study, 3M Environmental Laboratory, AMDT-041598.1 d) Memorandum from Marvin Case, regarding histopathology review of liver tissue in Covance Study 6329-222, July 27, 1998 5) 26-Week Capsule Toxicity Study with Perfluorooctane Sulfonic Acid Potassium Salt (PFOS: T-6295) in Cynomolgus Monkeys, Covance Laboratories Inc., Study Number 6329-223. In progress. a) Undated report covering the 26-week dosing phase and one year of recovery. b) John Butenhoff, "Dose-Setting Rationale for Six-Month Chronic Oral Study in Cynomolgus Monkeys," dated July 29, 1998 and followup Aug. 3, 1998. c) Fecal Urobilinogen Analysis, Mayo Clinic, Porphyrins and Nutritional Chemistry Group, Test Code: 8308. i) Summary Report from Dr. Joseph P. McConnell, dated March 16, 1999. ii) General information from Mayo Clinic, Porphyrins and Nutritional Chemistry Group on Urobilinogen Analysis, dated January 14, 1999. iii) Individual animal urobilinogen lab reports (raw data) from Mayo Clinic, Porphyrins and Nutritional Chemistry Group. d) Pathology Report (Ancillary Study), Electron Microscopic Evaluation of Liver in Cynomolgus Monkeys, Pathology Associates International, Study No. EM99.76, July 13, 1999. e) Pathology Review, Marv Case to Andrew Seacat, dated July 22, 1999 relaying the results of a histopathology review of slides. 000017 Attachments to Letter to C. Auer dated May 4, 2000 Perfluoroctane Sulfonate Studies f) Laboratory Report, Interim Report of Preliminary Data for 26 Week Capsule Toxicology Study with PFOS in Cynomolgus Monkeys, 3M Environmental Laboratory, Report No. FACT-TOX-030, dated March 29, 1999. 6) Two Week Oral Rangefinding Toxicity Study of T-2509CoC in Rats, Safety Evaluation Laboratory, Riker Laboratories, Inc., Experiment No. 179RR023, 3M Reference no. T-2509.3 (FC-99 New Formula, L-4509, 25 % diethanolamine salt of perfluorooctanesulfonate in water), February 25, 1980. 7) [Submitted under claim of Confidentiality] d. ^-M i 0v*- tfw. ^ / Jsr p/oS 000018 Attachments to Letter to C. Auer dated May 4, 2000 Perfluoroctane Sulfonate Studies Teratology Pilot Teratology Study in Rats, T-3551 Final Report, May 13, 1983, 3M Reference T3551.12 000019 Attachments to Letter to C. Auer dated May 4, 2000 Perfluoroctane Sulfonate Studies Pharmacokinetic Studies 1) Skin Absorption Studies on Surfactants (1983) a) Report from W. C. McCormick to D. R. Ricker, dated September 26, 1983 summarizing data b) 28 Day Percutaneous Absorption Study in Rabbits with FC-95, Safety Evaluation Laboratory, Riker Laboratories, Inc., Experiment No. 0979AB0632 (FC-95) c) 28 Day Percutaneous Absorption Study in Rabbits with FC-99, Safety Evaluation Laboratory, Riker Laboratories, Inc., Experiment No. 0979AB0633, 3M Reference No. T-3988.1 (FC-99, diethanolamine salt of perfluorooctanesulfonate, assumed to be 25 % in water) 2) Single-Dose Intravenous Pharmacokinetic Study of T-6053 in Rabbits, 3M Environmental Laboratory (FC-99, diethanolamine salt of perfluorooctanesulfonate in water Lot 130, Unit 177. 0.04 % FC solids in water), November 16, 1995. Final Report - Analytical Study, which includes copy of in vivo Study No. AMDT010495.1, Hazleton Wisconsin, Inc., Project No. HWI 6329-136, 3M Reference No. T-6053.1 3) Single-Dose Dermal Absorption / Toxicity Study of T-6053 in Rabbits, 3M Environmental Laboratory, Study No. AMDT-022195.1 (FC-99, diethanolamine salt of perfluorooctanesulfonate in water Lot 130, Unit 177. 0.04 % FC solids in water), November 22, 1995. Final Report - Analytical Study, includes in vivo Study Hazleton Wisconsin, Inc., Project No. HWI 6329-137, 3M Reference No. T6053.2 4) Fluorochemical (FC) Levels in Naive Rats, 3M Medical Department, Toxciology Services, Study No. T-6316.9, DT21, Draft Report for Objective 3, May 14, 1999. 5) Analytical Data submitted to Dr. Jennifer Seed, USEPA, by letter dated May 3, 2000, including serum measurements from two in-life studies: a) Analytical data from Advanced Bioanalytical Services Study No. FACT-TOX111, with respect to Oral (Gavage) Pharmacokinetic Recovery Study of Perfluorooctane Sulfonate in Rats, Argus Laboratories Protocol No. 418-015, 3M Reference T-6295.14. 000020 Attachments to Letter to C. Auer dated May 4, 2000 Perfluoroctane Sulfonate Studies b) Analytical data from Advanced Bioanalytical Services Study No. FACT-TOX110., with respect to Oral (Gavage) Pharmacokinetic Study of Perfluorooctane Sulfonate in Rats, Argus Laboratories Protocol No. 418-013, 3M Reference T6295.12. In Vitro Comparative Metabolism Study in Rat and Human Hepatocytes with Various Fluorochemicals, 3M Reference T-6295.1, study of T-6292 (N-ethyl FOSE), T-6293 (N-ethyl FOSE monophosphate ester), T-6294 (N-ethyl perfluorooctane sulfonamide), and T-6295 (Perfluorooctane Sulfonate) a) Range-finding Cytotocity Assay, SRI International Toxicology Laboratory, Study No. B010-95 - protocol and faxed results dated Oct. 26, 1995, Dec. 12, 1995, and Jan. 16, 1996 b) Metabolism of T-6292, T-6293 , T-6294, T-6295 by Rat and Human Hepatocytes, SRI International Toxicology Laboratory, Study No. B011-95 c) Advanced Bioanalytical Services, Inc., Analytical Report, Additional Characterization of Metabolites of T-6292, T-6293 and T-6294 from Rat and Human Hepatocytes by TurboIonSpray LC/MS and LC/MS/MS. SemiQuantitative Analysis of T-6295 in Rat and Human Hepatocytes Incubated with T-6292, T-6293 and T-6294 by LC/MS/MS, January 28, 1998, Report 98AGKP01.3M d) Working Interpretation of Results, chart entitled Perfluorosulfonamide Metabolism in Rat vs. Human Hepatocytes, updated Feb. 5, 1998 based on ABS Jan. 1998 report 000021 Attachments to Letter to C. Auer dated May 4, 2000 Perfluoroctane Sulfonate Studies Mechanistic 1) Reports from University of Minnesota Duluth Research (Kendall Wallace): a) Kendall B. Wallace, Biochemical and Molecular Mechanistic Studies of N-Alkyl Perfluorosulfonamides, Research Proposal, April 8, 1997, and Updated Proposal May 7, 1998 b) Kendall B. Wallace and Anatoli Starkov, The Effect of Perfluorinated Arylalkylsulfonamides on Bioenergetic s of Rat Liver Mitochondria, Feb. 4, 1998 c) Report on Covance Studies, assessment of mitochondrial bioenergetics, undated d) Summary of the Effects of PFC's [Perfluorinated Compounds] on Mitochondrial Bioenergetics In Vitro, undated e) Report, Effects of Selected Perfluoro-compounds on Mitochondrial Beta- Oxidation, Dec. 20, 1999 f) Report, Effect of Acute FC Administration on Catalase and acylCoA Oxidase Expression, January 27, 2000 2) Nabbefeld, et al., Displacement of a Fluorescently Labeled Fatty Acid Analogue fromFatty Acid Carrier Proteins by Wyeth - 14,643, Ammonium Perfluorooctanotate, Potassium Perfluorooctane Sulfonate and Other Known Peroxisome Proliferators, Abstract, Society of Toxicology, 1998 Annual Meeting 000022 Attachments to Letter to C. Auer dated May 4, 2000 Perfluoroctane Sulfonate Studies Human Sera/Medical Surveillance/Epidemiology Memorandum from D.E. Roach and S.D. Sorenson, 1983 Decatur Blood Fluoride Review, January 20, 1984 Antwerp Blood Testing Results from June 1995, by Jeffrey H. Mandel, M.D., M.P.H., and Jean Burris, R.N., O.H.N., November 6, 1995 Analysis of Serum Values in Decatur Workers, prepared by Michel Burlew for Larry Zobel, M.D. and Jeffrey Mandel, M.D., April 2, 1998 Laboratory Report, Analysis of FCs in Samples of Children's Sera, Laboratory Report No. FACT-GEN-011, 3M Environmental Laboratory, May 21, 1999 Laboratory Composite Report, Analytical Reports of Data for Fluorochemical Analysis in Human Sera, LIMS No. 1623, 3M Environmental Laboratory, April 28, 2000 000023 Attachments to Letter to C. Auer dated May 4, 2000 Perfluoroctane Sulfonate Studies Genotoxicity 1) Mutagenicity Evaluation of T-2014 CoC in the Ames Salmonella/Microsome Plate Test Final Report, Litton Bionetics Project No. 20838, Protocol No. DMT-100, February 20, 1978. 2) Memorandum Report from S. R. Rohfing to A. N. Welter, dated March 31 1977, on Results of the Ames Spot Test for Mutagenicity screening of various FCs, including Sample 12-583 which is FC-95, Notebook Reference 45867-24, 25. 3) Mutagenicity Test on T-6295 in an in vivo Mouse Micronucleus Assay, Final Report, Corning Hazleton , Inc. (CHV), CHI Study No. 17403-0-455, May 23, 1996, and protocol and amended protocol. 4) Final Report, Chromosomal Aberrations in Human Whole Blood Lymphocytes with PFOS, Covance Laboratories, Inc., Covance Study No. 20784-0-449, 3M Reference No. T-6295.18, October 25, 1999. 5) Final Report, Unscheduled DNA Synthesis in Rat Liver Primary Cell Cultures with PFOS, Covance Laboratories, Inc., Covance Study No. 20784-0-447, 3M Reference No. T-6295.19, November 9, 1999, and protocol. 6) Final Report, Salmonella-Escherichia co/z/Mammalian-Microsome Reverse Mutation Assay with PFOS, Covance Laboratories, Inc., Covance Study No. 20784-0-409, 3M Reference No. T-6295.17, November 5, 1999, and protocol. 7) Final Report, In Vitro Microbiological Mutagenicity Assays of 3M Company Compounds T-2247 CoC and T-2248 CoC, SRI International, SRI Project No. LSC-4442-016, 3M Refemece No. T-2247.1 (FC-99 Old Formula, L-4299 which is 50 % of the diethanolamine salt of perfluorooctanesulfonate in water), September 5, 1978. 8) Prof. Nicola Loprieno, "Evaluation of Mutagenicity Studies Developed on (PFOS) Perfluorooctane Sulfonate," prepared at the request of John L. Butenhoff, Ph.D., 3M Corporate Toxicology, January, 2000. 9) Final Report - Bacterial Reverse Mutation Assay of t-1 , Hita Research Laboratories, Chemical Biotesting Center, Study Code KOI-1802, 3M Reference No. T-6667.1 (FC-98, Potassium Perfluoroethylcyclohexyl Sulfonate), September, 1996. 000024 Attachments to Letter to C. Auer dated May 4, 2000 Perfluoroctane Sulfonate Studies Ongoing Research/Study Protocols 1) 104-week Dietary Chronic Study and Carcinogenicity Study with Perfluorooctane Sulfonic Acid Potassium Salt (PFOS: T-6295) in Rats, Covance Laboratories Inc., Study Number 6329-183. In progress. Interim data provided. 2) 26-Week Capsule Toxicity Study with Perfluorooctane Sulfonic Acid Potassium Salt (PFOS: T-6295) in Cynomolgus Monkeys, Covance Laboratories Inc., Study Number 6329-223. In progress. Interim data provided. 3) Protocol for Study: Low Level PFOS Dose versus Rat Serum and Liver PFOS, 3M Medical Department, Corporate Toxicology, Study No. T-6295.16 DT31, October 29, 1998. (Study in progress.) 4) Protocol for Study: Pharmacokinetic Study of POSF in Rats, 3M Medical Department, Corporate Toxicology, Protocol for Study No. T-7098.1, January 7, 1999. (Study in Progress). 5) Study Plan, ST-43: Standard Procedure for Liver Subellular Fractionation, undated, 3M Toxicology Laboratory 6) Plan for Study Nos. T-6295.23; ST-46, Exploratory In-Vitro Pervutaneous Absorption Study of Theophylline, Salicylic Acid, Perfluorooctylsulfonate, and Ammonium Perfluorooctanoate in SkinEthic Reconstituted Epidermis Model, May 4, 2000, 3M Toxicology Laboratory 7) Study Plan, Luebker, Perfluorooctane Sulfonic Acid Induced HMG-CoA Reductase Inhibition in Pregnant Rats and Rat Pups, January 21, 2000 00002S Attachments to Letter to C. Auer dated May 4, 2000 Perfluoroctane Sulfonate Studies Planned Studies 1) Preliminary Study Outline, One Generation Reproduction Study of PFOS in Rats, Pharmacokinetic Analysis, May 3, 2000 2) Preliminary Study Outline, One Generation Reproduction Study of PFOS in Rats, Mevalonic Acid/Cholesterol Challenge and NOEL Investigation in Rats, April 27, 2000 000026 ATTACHMENT TO LETTER TO C. AUER DATED MAY 4,2000: PLANNED ENVIRONMENTAL STUDIES ON PERFLUOROOCTANESULFONATES Environmental Fate and Transport Soil Adsorption/Desorption, 3M Environmental Laboratory. Start date: May 2000. Protocol in progress. Multi-Media Modeling of PFOS Distribution, Mackay, D. (Trent University) Start date: June 2000. Protocol in progress. Bioconcentration Factor, Wildlife International, Ltd. Start date: Sept. 2000. Protocol in progress. Ecotoxicity Elements Acute Toxicity to Bluegill Sunfish, Wildlife International, Ltd. Start date: July 2000. Protocol in progress. Acute Toxicity to Sheepshead Minnow, Wildlife International, Ltd., Start date: July 2000. Protocol in progress. Phytotoxicity - Vegative Vigor and Plan Uptake, Wildlife International, Ltd. Start date: June 2000. Protocol in progress. Acute Toxicity to Eiseiniafoetida (Earthworms), Wildlife International, Ltd. Start date: June 2000. Protocol in progress. FETAX (Frog Embryo Teratogenesis), Wildlife International, Ltd. Start date: June 2000. Protocol in progress. Mallard Duck Reproduction (Dietary), Wildlife International, Ltd. Start date: July 2000. Protocol in progress. Bobwhite Quail Reproduction (Dietary), Wildlife International, Ltd. Start date: July 2000. Protocol in progress. Environmental Monitoring (Environmental Sampling & Release Estimation) Estimation of PFOS in Life-Cycle Waste Streams, Battelle Memorial. Start date: May 2000. Protocol in progress. Estimation of Life-Cycle Releases, Battelle Memorial Institute. Start date: January 2001. Protocol in progress. 000027 Carpet Release Study, Battelle Memorial Institute. Start date: June 2000. Protocol in progress. Multi-City Study, Centre Analytical Laboratories, Inc.; 3M Environmental Laboratory. Start date: June 2000. (Study plan being provided to EPA) Multi-City Study - Analyses of Sediments. Start date: June 2000. Multi-City Study - Analyses of Water Columns. Start Date: Sept. 2000. Multi-City Study - Analyses of Surface Water Film. Start date: Sept. 2000. Multi-City Study - Analyses of POTW Effluents. Start date: Sept. 2000. Multi-City Study - Analyses of POTW Sludge. Start date: June 2000. Multi-City Study - Analyses of Landfill Leachates. Start date: Sept. 2000. Multi-City Study - Analyses of Fish. Start date: June 2000. Multi-City Study - Analyses of "Market Baskets." Start date: June 2000. 000028 ATTACHMENT TO LETTER TO C. AUER DATED MAY 4,2000: PRE-1976 TOXICOLOGY STUDIES ON PERFLUOROOCTANESULFONATES Eye and Skin Irritation Studies Report on Sample T-l 117, Warf Institute Inc., Project No. 4102871, November 7, 1974, and explanatory correspondence indicating material is FC-95 (Perfluorooctane Sulfonate potassium salt) Eye, Skin and Acute Dermal LD50 Study Report on Sample T-991 (FC-93, L-3356, Ammonium Salt of Perfluorooctane Sulfonate, 25% in 20% Isopropyl Alcohol and 55% Water), Warf Institute Inc., Project No. 4053862, June 25, 1974 000029 e Attachment to Letter to C. Auer Dated May 4, 2000: Environmental Studies on Perfluorooctanesulfonates (Post-1975) Physical/chemical Properties Title Determination of the Melting Point/Melting Range of PFOS; Boiling / Point (Not Conducted) Determination of the Vapor Pressure of PFOS Using the Spinning 2 - Rotor Guage Method PFOS: Determination of the n-Octanol/Water Partition Coefficient y by the Shake Flask Method - A Non-GLP Feasibility Study in Support of Wildlife International, Ltd. Project Number: 454C-1Q8 Laboratory or Author Wildlife International, Ltd. Wildlife International, Ltd. Wildlife International, Ltd. Completion Date 2/24/99 Type Robust Summaries, Final Report, Protocol 5/5/99 Robust Summary, Final Report, Protocol 2/11/00 Robust Summary, Feasibility Study Testing Results: Air-Water Partition Coefficient (KAW) for PFOS 1- 3M/Wildlife International, Ltd., U of Trent f- Determination of the Water Solubility of PFOS by the Shake Flask Wildlife Method International, Ltd. Technical Report. Solubility Measurements on FC-95 3M Env. Lab Solubility Estimate of FC-95 by use of Xertex TOC Analyzer 4" Xertex, 3M Env. Lab 3/19/99 4/26/00 2/6/81 6/29/82 Robust Summary, Letter Report Robust Summary, Final Report, Protocol Brief Robust Summary, critique from Endwin Tucker (3/1/93), Final Report Brief robust summary, fetter report Environmental Fate and Transport Title AdsortHjon of FC 95 and FC 143 on Soil (Note: the 3M Env. Lab summary'iSstitled: Summary of the Soil Adsorption study of the Potassium SalTetf^erfluorooctanesulfonic acid, 7/22/98) FC-95/PhotolysisStudy Using SimulatedSunlight. (Note: the3M Env. Lab summary is titled: summary of Photolysis Study Using Simulated Sunlight on the Potassium Salt of Perfluorooctanesulfonic acid) Biodegradation Studies of Fluorocarbons (8/12/76) report and Biodegradation Studies of Fluorocarbons - III (7/19/78) report. (Note: both reports summarized with one robust summary) BOD/COD results for FC-94-X (Li salt of PFOS) BOD/COD results for FC-99 (DEA salt of PFOS) Transport between environmental compartments (fugacity modeling) included in letter from Don Mackay on the air/water partitioning coefficient calculations Analysis for fiuorochemicals in Bluegill fish. Laboratory or Author 3M Env. Lab 3M Env. Lab Completion Date Type 2/27/78 Robust Summary, 3M Env. Lab Summary, Comments from Stephen A Boyd from MSU, Final Report 1/9/79 Robust Summary, 3M Env. Lab Summary, Final Report 3M Env. Lab 8/12/1976, 7/19/78 8rief Robuat Summary. 2 Final Reports Pace Analytical ^ '^ 3 /3 0 /9 4 3M Env. Lab 6/8/79 Computer-generated Summary of Testing Results .Robust Summary and final Reports DMER No date Robust Sbiqmary, letter report's. 3M Env. Lab 5/17/79 Robust Summary, Technical Report 000030 PHYSICAL - CHEMICAL PROPERTIES A Robust Summary, Final Report, and Protocol on the physical-chemical properties of perfluorooctanesulfonate are included for each of the following parameters: PARAMETER DATE OF REPORT RESULTS Melting Point/Melting Point Range Vapor Pressure n-Octanol/Water Partition Coefficient Air-Water Partition Coefficient Solubility in pure water Solubility Measurements on FC-9S 2/24/99 5/5/99 2/11/00 3/19/00 5/3/99 2/6/81 - 400C 3.31 x 10'4P@20C Not calculable; three phases 0(<2xl0'6) 570mg/l 1080mg/l The data presented in the study "Solubility Measurements on FC-95," was determined by indirect measurement, not by actual analysis. Therefore, the data is not reliable. Please note that the March 1,2000 submittal to EPA entitled "Sulfonated Perfluorochemicals in the Environment Sources, Dispersion, Fate and Effects" included solubility data on water other than pure (i.e., fresh water; filtered sea water; unfiltered sea water). These data were developed, however, in support of other studies and not produced using GLP Standards. For this reason, Robust Summaries, Final Reports, or protocols for this specific data are not being provided. 000031