Document Kg29Xb4RR4vmwBy7YZjo2KK6

? t, Avb/S&T/ujwst' Review and Audit of IBT Mo. 563-03222, Chronic Vapor Inhalation Toxicity Study with vinyl Chloride '(Ethylene Derived) in Albino Rats, Albino Mica and"Golden Hamsters for the Manufacturing dTenusts Association The review and audit of this study was conducted at IBT's offices in Northbrook, H, April 14 to April 13, 1930. The review and audit on this study concentrated on the con duct and monitoring of the exposures. However, attention was also devoted to the source of the animals and body weight data as they affected fate and mortality. In addi tion, the review and audit included inspection of hand written observation books reflecting exposure concentrations, as well as gas chromatographic charts where they were avail able, hand written and computer body weight printouts, hand written mortality logs, hand written observations and cor respondence . CORRESPONDENCE, PROTOCOL AND TIMETABLE The timetable indicates the exposures were to be ini tiated on 9/10/73 for the T-I, T-IX and T-IIX groups and on lQ/1/73 for the T-IV groups. The termination of the exposures for the first three groups was to be at 9/3/74- and for the T-IV group at 9/27/74. The animals were then to be held for a year prior to terminal sacrifice. A letter dated June 15, CONFIDSUTIAL CHA 0 1 0 9 9 8 I3T No. 553-03222 -2- 1973 from Or. Torkelson of Dow railacta a sita visit was made by tha MCA Task Fares, viaiting1 tha Decatur research labs of I3T where tha soudy was conducted. Quastions were raiaad about tha adequacy of equipment, the exposuras main tained, the exposure concentrations, the question of contam ination and record keeping. A. major concern was raised about tha adequacy of the analyses and record keeping. Hand written notes alongside the margin of this latter indicate that certain corrective actions were taken at X3T. r was it"able to tall whether there were any other documented site visits. In addition, a progress report issued October 13, 1973 after about a month of exposure, reflects adjustments that were made to compensate for tha issues that were raised during the MCA site visit. &NXMAL5, HOUSING AND HUSBANDRY The animals for this study were supplied by the Charles River Breeding Laboratories. Unfortunately, we are not able to identify specific animal receipts with this project. However, we were able to identify from September invoices rsceipts of hamstsrs as raplacsments without charge for ani mals that were dead on arrival in the August shipment. We were able to identify an additional receipt of hamsters in early November, 1973, two months after the study started, which indicatsd 130 males and 130 females were received at that time. Y'-' COl'nrTnv'ffm* i c BOB WEST ASSOC I Ai r^. I3T MO. 633-03222 -3 Inspection of the body weights early in the study in dicates despite the report not reflecting the mortalities that there was a rather- high mortality in the hamsters early in the study. At four and five months there were extensive replacements of the hamster groups. The animals were ear punched and the toes removed so . the animals could be individually identified. This was to allow for identification for individual body weight determin ation and identification of when the animals died. because the animals were group housed in groups of 3-? in stainless steel cages. Inspection of some of the hand written notes shows the layout of the rats in the raclt and indicates that extra animals were to be spaced evenly throughout the rack. In addition, another hand written sheet shows the arrangement of the gang cages in the chambers. There were 13 gang cages, 3 rats each, to equal 144 rats. However, the protocol only calls for 10Q rats at each level.. This procedure allows for ample opportunities for replacement and substitution. Despite the fact that the animals were identified by ear punching- and toe removal, most of the time the animals were not weighed individually but rather by the cage, e.g., 3-5 animals. Numerous hand written notes showing the arrangement of some of the animals in the racks reflect the animals excaped or were cannibalized, along with mortality dates for the remain ing individual animals. .confidential FuEJect to Protective Order in Jtoss. v. J7onogo, Inc.. ITa. 90-4337 14th Judicial District Cou^t Calcasieu Parish. Loulsiufi* BOB \V EST ASSOC I AT ES. INC. CMA 011000 I3T No- 333-03222 -4 vtnel chloride , source, identity Vioyl chloride (ethylene baaed) appears is have been supplied as, a.?., containers in excess of 500 pounds, by PPG Industries, Industrial Chemical Division, Lake Charles, LA. A letter from w. 3. 3raybill of PPG to Dr. John Goode of Bio-Test in Decatur indicates that one cylinder would be required per month. Heel sample analyses from the Bio-Test, residues were also performed by PPG at the Lake Charles, LA operation and those that were available appeared to be in good agreement with the new sample analyses supplied to BioTest. Unfortunately, there was no inventory log at 3T for vinyl chloride. PPG personnel should be ashed for account ability of the vinyl chloride sent to I3T. In addition, they would also have records of the returns for heel analyses. GENERATION OP VINTL CHLORIDE VAPORS The vinyl chloride supply tanks were kept in an explos ion room. The material was piped to the test chambers through thin-walled stainless steel tubing. Before the thin-walled tubing delivered the vinyl chloride to the chambers, it was connected to a micrometering valve and a glass rotometer to control and monitor the supply of vapor to each chamber. Vapor-air mixture entered the top of the inhalation chamber, circulated through the chamber and was- exhausted at the bot tom* Unfortunately, we were unable to find a daily log for CONFIDENT tat. "oteotive Order in' - ^oc_z_Inc^0. 90-4837 "lal District Court u ParishnkLouisiana BOB WEST ASSOCIATES. INC. CHA 011001 X3T. NO. S33-03222 the weighing or measuring of the vinyl chloride that was dispensed during each daily run. CHAMBER MONITORING There- are records of standardizations being conducted in September of 1973, covering the range from 20 to 100 ppm standardization and from 50 to 400 ppm for the 200 ppm standardization and from 400-4000 ppm for the 2500 ppm level. Standardizations were performed at approximately weekly in tervals prior to that point, where the automatic- sampling, system was hooked up to the on-line gas chromatograph record er. The initial samples from the chambers for the first part of the study (six. weeks) were obtained by use of grab, samples which were than analyzed by the gas. chromatograph. w were unable to find any record of daily sample analyses being taken in the research books for the month of October, 1973, the second month of the study, other than ranges and mean, concentrations.. There also was no evidence of chamber temp erature or humidity being recorded. General Observations The following observations were obtained by inspection of research log books which, present means and ranges of exposure concentrations, time interval sampling for the gas chromatographic concentrations and charts, as well as the hand written observations in the research log books. CONFIDENTIAL SuUJeot to Protective Order in Boss v. Conoco, Inc., Ho. 90-4337 14th Judicial District Court Calcasieu Parish, Louisiana BOB WEST ASSOCIATES, INC. CMA 011002 I3T Mo. S33-Q3222 6 1. Most at the time the chambers were not on for sir hours. 2. MOst of the time the chambers were not even monitored for five hours, in some instances they were only moni tored for one to two hours. 3. In some instances there-was selected data for the daily exposure concentration, e. g., values on the low side may have been eliminated or values on the high side may have been eliminated. 4-. Periodically, there were mechanical problems associated with the rotometer, e.g., broken- or clogged or unclean lines. 5. Some days there was no exposure because of equipment failure or TV filming or only a one-hour exposure was conducted. 5. There was evidence that the chambers did not have an adequate clearance time, since measurements made 45 minutes after termination of the exposure still showed rather high levels of vinyl chloride present. T. There was evidence of contamination in the solvents room and the pilot plant which were in close proximity to the- exposure room. 3. Some days there was no analytical data recorded for the exposure concentrations* 9. In some cases, the research log hook showed means and ranges.,.exposure concentrations, with no raw supportive CONFIDENTIAL 'Subject to Protective Order n toss V. Conoco. Inc.._ No- 90-4o- 14th Judicial District Court Calcasieu Parish, Louisians BOB WEST ASSOCTAXES. INC CMA 011003 I3T Ho. 533-03222 -7 data shown for periodic sampling with the gas chromato graph. 10. In some instances, the SO ppm nominal concentration may have reached a concentration as high as 100 ppm and, in one instance, as high as 400 ppm which is not reflected in the means or ranges presented in the interim report.. 11. In some instances, the control chamber indicated levels of vinyl chloride in a range of 5-11. S ppm. 12. In September of 1974, near the end of the year of expos ure, there was some research book data reflecting exposure concentrations but no supportive gas chromato graphic data. 13. In some cases there is no data available for one or two levels out of the four exposure chambers being run. 14. There were periodic problems with the gas chromatograph itself, either with the Ignitor, Detector or the Recorder. 15. In some instances there were means and ranges presented in the-research book but no interval data ware available. 15. In some instances, the chamber may have been on for four or five hours yet gas chromatographic readings - were only taken for two hours. 17. There were a number of instances where the data presented in the research book do not agree with the data shown on the gas chromatographic chart. V-" CONFIDENTIAL Subject to Protective Order in Ross v. Conoco, Ine.. Ifo. 90-43J/ 14th Judicial District Court 1 Calcasieu Parish, Louisiana BOB WEST ASSOCIATES. INC. CMA 01100-4 I3T Mo. S33-03222 -8- Soecific Exposure Observations The following notations were associated with. the spe cific exposures and may also be used together with the inhalation chamber concentrations presented in Table I. The exposure concentrations presented in Table I are those presented by 23T and one can see with the use of these spe cific notations that the values presented in Table I are not an accurate reflection, in many instances, of the exposure concentrations or are based on minimal supportive analytical data. Exposure Muznber 5 20 s 35 33 Comment The values for the T-I mean and the range for the raw data in the report do not agree. In the daily intervals, there were problems with sampling and the curve does not appear to be of value. 200 ppm equaled 400 ppm. The gas chromatographic charts are no good and the research book, concentration is not shown or recorded The gas chromatographic chart agrees with the datain the research book. Thera are no gas chromatographic charts but there is data in the research book. BOB WEST ASSOCIATES. INC. CMA OU 005 I3T No._ 633-0 3222 -9- Exposure Number 35 36 108-110 134&135 141 144 145 Comment There are no T-I or T-n data on the chart or in the book. T'-IXX data is present in the research book but not on the gas chromatographic chart. The gas chromatographic chart and the data in the research book agree but the exposure concentra tions are on the low side. The gas. chromatographic charts do -not match the data in the log book. Observations were noted that some of the data was off the GC chart and there were some operator errors and missing data. Thera were no GC charts to go with"the data pre sented in the research books. No charts available for the exposures 110-136. A line was drawn through the first hour values and apparently discarded. There are gas chromatographic charts and research book data available but another notebook says that exposures were not run. In addition, the control line shows contamination. A. notation was made that some of the readings were off the chart -- wide excursions on both the low and high sides. In addition, the controls were contaminated. confide:7" i at, Subject to Frotactivo C?r?~tv ^ Eoss_v. Conoco, Inc., To. 90-4/s?7 14th Judicial District Court Calcasieu Parish. Louisiana BOB WEST ASSOCTATES. INC. CHA 011006 I3T. No. 633-03222 -10- Exposure Mumber Comments 146 Soma of the data from the charts has bean dis carded. 150 The T-II value at. the first sampling interval does not agree with the data presented in the research ' book- The negative control line also shows con- * tamination. The T-IXX value is on. the high side and. reaches 3420. 152 A broken rotameter on line T-I. The values for the 1 T--III line indicate they are not working and they have been deleted. The control background is high. Selected data has been used for the tabulated data presented. 153 Analytical samples are present for four hours. There are missing data when one compares the GC charts versus that present in the research book. 159 The chambers were only on for two hours. There are no exposure concentrations for T-IX. T-IXX and T-IV at the first sampling. There are- incomplete values for the second* sample at two hours. 155 The was operated for 4 1/2 hours. Zero time was taken ten minutes after the start of the exposure, at which time the gas chromatographic readings were taken. Environmental samples on the C0IIFIE3NTIAI, Subject to Protective Or^er in -gosa V. Conoco._lnc. . i:o. 90-4837 14th Judicial District Court Calcasieu Parish., Louisiana BOB WEST ASSOCIATES. INC. CMA 011007 I3T No. 633-03222 -11- ExpO 3 ure Number 165 (cont'd) 166 Comments roof appeared high. 3ood efficiency, where the syringes were cleaned was poor, vinyl chloride was still detectable in the T-rv chamber 45 min utes after the exposure was terminated. The solvent, room and the pilot plant both showed vinyl chloride contamination. The first reading on the gas chromatographic chart is 3:55 pm. The- readings continue until 4:40 pm at sharply lower concentration. The chamber was turned on at 10 o' clock in the mom- 169 Sampling was only conducted for 4 hours. The TT-in value was 3710 ppm. For the T-I and T-IX lines, there was no sample taken for the second sampling interval, or the fourth interval. 130 Basically no analytical data on exposure concen tration. 196 Exposures were sampled for five hours. Selected- data in the daily range and no noon samples. The control line was contaminated. 197 The chambers ran for five hours and GC data are available for three. Suhjoot to Prote v. Conoco, i- 14th Judicial r Caleasieu^Paxish,, Louisiana BOB \V EST ASSOC I AT ES. INC. CMA 011008 I3T No. 533-03222 Exposure Miuntaar CQtwman-t 201 A seven-hour exposure is indicated as having bean run. The sampling far GC analysis was run far somewhat less than five hours. Selactad data has bean taken at the initial reading and then spor adic data is missing throughout the run. 206 The chambers were not monitored and no data are available. 210 No sampling. Exposures were only on for one hour with a notation o TV filming. 213 There was one reading for each line for the first two hours, obtained manually. Half of the samp lings were done on automatic, but two, rather than three, samples were obtained. 224 Notation is made of CBS filming tomorrow; expos ures were run for 4 1/2 hours. The line control had a reading of 6. The GC chart shews no agree ment with the research book, which is sparse. However, it does show T-XII range from 3-320 ppm. The T-T SO ppm range equals 445 ppm. 225 No gas chromatographic charts to support the mini mal data in the research book. The chambers were COH^ir^NTIAL turned on at 9; 00 am and were off at 11:40 am~ GC readings were taken only at 10:00 and 11:00- am. 53 7 BOB WEST ASSOCIATES, INC. CM/S 011009 I3T Ho. 633-03222 -13- Exposure dumber 223 231 263&217 2334239 243 244 251 255 Comment For T-I and T--IX, there are missing data. For T-IXX, there are no values for the first and second samplings. One-third of the values which were on the low side, were deleted from the calculation of the averages, one of which, was off the graph. Se lected. data, i* shown. The control shows a peaic height of 7.5, indicating some contamination. The gas chromatograph was not working; no data. but have been reported for one value at one interval.. The research book shows a mean, and ranges but no * supportive data-were available. The line control shows a peak height of 3. One- third of the data from the GC charts were not used. in the- calculations or averages in the research book. Samples are only shown for three hours. The GC chart data is sparse. The line control is shown as 6.5. The 50 ppm equaled 400 ppm and a notation of major problems. Air recirculated was causing high concentrations in the control chamber. The chambers were turned CCNFI^riTIAX. Sub'Ject to Protective Crdar in Ross v. Conoco, Inc.. Ho. 90-4337 - 14th Judicial District Court V. .Calcasieu Parish,, Loulaian* R()R WEST ASSOCIATES, INC. CMA OHOIO I3T. Ho- 63 3-0 3222 -14- Sxposure Mumb^y 255 (coat1d) 257 253 Comment off. The control chamber had a level equal, to 3.4 opm of vinyl chloride. A notation of definite leakage into the roam waa recorded. Selected data only for two hours of the exposure was used. The T-T7 chambers were turned on two hours after the res-t of the chambers. The line control shows vinyl chloride- contamination. One of the 50 ppm values is equal to at least 100 ppm. In addition,, one-third of the values were unavailable and se lected data were used. The first exposure concentration readings were taken at 1:15 pm and also at 2:15/ despite the fact the chambers were turned on at 11:30 in the morning. Most of the data were incomplete. This appeared to be the last exposure where gas chroma tographic chart data were available. The research book has data -for exposures 253/ 230/ 231/ 262/ but there are no sup portive gas chromatographic data. CMrt 1011 - --- BOB WF.ST ASSOC LATHS, INC. I3T So. 533-03222 -15- BODY WEIGHTS Body weights were available, either as hand written calculations or as computer printouts. Unfortunately, many of the computer printout body weight tabulations reflected the animals being weighed on a gang-cage basis. Therefore, it becomes more difficult to determine, substitutions. . a tabulation beginning with month 5 and proceeding through month 13 is shown, below for the body weight data.. This com pares the data presented in the interim IBT reports versus that obtained when one inspects the computer printout itself. These data have only been examined for the T'-XXX group. MEAN BOUT WEIffiTS FROM PRINTOUTS VINYL. CHLORIDE RATS, T-IIX - 2,000 PPM- Month Male Pamela Month Male 5 Report 549 321 Printout 550.5 312 9 Report Printout 631 359 627 359 6 Report 567 329 Printout 567.3 339 10 Report Printout 663 356 629 356 7 Report 565 . Printout* 714 691 702 606 346 436 11 Report Printout 12 Report Printout 696 680 736 `7ia 374 374 -373. ` 378 3 Report 585 Printout**" 581 359 359 13 Report Printout 740 420 729 420 *A11 values shown on printout **Subtracted 329 grans from printout mean Inspection of these date indicates that during the seventh month there is a major difference between the body weights in the printouts and those presented in the final report, suggesting replacements. s BOB WEST ASSOCIATES. INC. CMA 011012 mprsmjty and pats Hand writ-tan shaats reflecting tabulations of mortali ties ara available. these sheets bear various fates at periodic intervals. Additional tabulation reflects animals that are missing or cannibalized, based upon the gang hous ing. However, there are numerous errors present, and inconsis tencies in these tabulations. A chart of missing rats, pre pared near the end of the third month of the study, shows numerous not accounted for in Or. Shah's tabulation. These differences are as follows: Untreated control male rats - 2 animals missing; none shown on Or. Shah's tabulation. Untreated control females - 3 missing; none shown on Or. Shah's tabulation. T-I male rats - 7 missing; 1 shown on Dr. Shah's tabulation. T--XI male rats - 5 missing; none shown on Dr. Shah's tab ulation. T-IXX male rats - 4 missing; none shown on Dr: Shah's tab ulation. T-i female rats - missing; none shown on Dr. Shah's. tab--. ulation. T-IX female rats - 2 missing; none shown on Dr. Shah's tab ulation. T-IXI female rats - 4-5 missing; none shown on Dr. Shah's tabulation. CONFIDE TIAL Calcasieu Parish, Louisiana CMA 011013 IBT No. 633-03222 -17- There ax numerous evidences of errors in the animal numbers or the animals being missexed,e.g., animal 751 female should be 633 male in the T-IXX group; animal 737 female should be male 691 in the T-IXX group; animal 733 female should be 692 male in the T-IXX group; animal 639, T-XXX male rah should be 636; 60?, T-XXX female rat, is a male. Autopsy sheets that reflect extra animals were avail able. A mortality tabulation prepared from the animals avail able in each group, as determined by the body weight print outs, beginning with month five and continuing through month 13 is shown in the accompanying Table. You will note a sub-- stantial difference in the numbers available as tabulated. between the printout and that presented in the report for the male T-XXX group, month 7, 12 and 13. Similarly substantial differences in the females in months 12 and 13, as well, also suggests substitution of the animals was occurring. Close attention was paid to determining the specific fate of the five animals examined in detail by .Or. Bussey in tfae.T-XXX male group, as reported to have brain tumors. inspection of the hand written post mortem tabulations by IBT staff, as opposed to the data presented on the autopsy sheets and the mortality data presented in the .final report indicates substantial discrepancies. BOB WEST ASSOCIATES. INC. CM/S 011014 1ST No. 533-03222 13 w !? i o', p " <P2 ^ C Q, Sn ^* n oaO <o0 ^ *+ S" ^n o o c 21 gCoc!D-+- \ <' H"- *H* <ID 2p_ fat* Animal #641, T-XXI mala, was found daad an 363 days, according to the report. Hand writ-ten ? ..'4. tabulation sheet shows the animal to be dead by 3/6/74, which is about 330 days into the study. The necropsy sheet shows the animal's date of death as 9/3/74 (3S9 days). These differences have not been explained. In the case of #649, the necropsy sheet and th report shows that the animal died at 402 days. The sheet presenting post mortem tabulations, dated 9/11/T4, however, shows the *nrml as being dead. This date would be approxi mately one year into the study. Animal #664 was reported as having died at 373 days. This " it is shown as being dead 7/17/74 on the necropsy sheet and as being dead on a hand written tabulation on 3/9/74 Therefore, the 373 day mortality presented in the report is incorrect and was probably 311 days. Animal #672, shown in the report as having died at 310 days, is also shown on a post mortem mortality tabulation dated 7/26/74 as being dead by that time. A necropsy date of 7/16/74 for this animal is noted. Therefore, the fate of ., this animal appears to be correct. In the case of arr-itnaJL $667, the report indicates- days orr test as 333 but the necropsy sheet is dated 6/27/74, re-- fleeting lass than a year on test. A post mortem log dated 6/26/74 also shows the animal as being dead. Therefore, the data presented as days on test in the report is in arrort BOB WEST ASSOCIATES. INC. CMA 011015 I3T No. 633-03222 . -19- CONCLCTSIONS An intensive audit of certain aspects of IBT No. 633-03222, chronic vapor inhalation toxicity study with, vinyl chloride, devoted to inspection of exposure concentrations and pertin ent body weight and mortality logs indicates the followingr (a) There are numerous evidences of replacement of animals in this study. Thera appears to be an exceptionally high replacement of the hamsters. Also, because of the- dif ferences in body weights and mortalities noted in the T-IXI rats, it is possible that there was major substi tution of the animals between the sixth and seventh months of the study and perhaps at 12 and 13 months. There are evidences of extra animals being available for the various species in the study. The accountability for the animals and the mortalities show substantial discrepancies. (b) The monitoring of the exposure concentrations and the conduct of the exposures shows poor technique and selec tion of data in many instances but does seem to indicate that a three-level study was conducted, even though the swings in exposure concentrations periodically may have been i 50-100%. The instances of actual overlap between exposure concentrations at the T--t level and' the T-IX level were relatively infrequent and there do not appear cony:y., Subject to Protective 0rd-)r 'n Sos s v ^Conoco, Inc. , No. 90 - a. 3 j 7 ' 14th Judicial District Court Calcasieu Parish, Louisiana BOB WEST ASSOC I AT ES. INC'. CMA 011016 13? Ho. 533-03222 20- to be any real instances of overlap between the T-II and T-IIX levels. (o) ?ha animals reported to have brain tumor3 indicate discrepancies as to their actual dates of death versus those reported in interim reports and in some cases on the necropsy sheets. In summary/ the study was conducted in an extremely sloppy fashion and one would have to look at any of the re sults. more from a qualitative standpoint than, a quantitative standpoint. CMA 0 1 1 0 1 7 aw/am BOB WEST ASSOC IATES. INC. -vo 90-430 rict Court Louisiana table j TEST MATERIALS Vinyl Chloride (Bthylene Derived) Chronic Vapt> ^Ohhlation Toxicity Study - Albino Rata, Albino Mice, and Golden Hamsters 'i Chamber Concentration Data Day ' Exposure TB-I Number Number _____Range_____ M?an Chamber Concentration IppwJ TE-II TE-II1 Ranee Mean Range Mean TE-IV** Range______ Mean 11 44 55 16 12 29 21 39 29 66 48 73 53 80 58 99 71 120 86 130 94 150 108 151 109 152 110 171 123 186 134 187 135 197 " 141 200 144 201 145 208 150 212 152 220 o 221 >3: 229 232 o 158 159 165 166 235 O 169 39 45 43 130 180 173 45 54 51 24 162 87 50 56 52 182 237 218 42 63 60 54 266 156 46 66 58 192 248 219 42 55 43 168 216 209 48 114 84 172 452 298 42 58 49 178 211 202 45 60 54 220 364 243 29 31 .-- 30 258 310 277 45 46 45 168 232 199 42 63 53 180 228 201 35 58 49 90 288 200 32 62 44 150 234 193 41.5 110 58.9 170 232 198 35 55 48 186 210 197 47 53 49.6 184 224 209 46.5 55.5 50.7 180 262 194 40 *55 45.1 178 216 196 38.5 56 i 49 178 310 223 37.5 55.5 48 174 212 197 48 54,5 51.4 170 242 200 36 64.5 48.1 154 282 194 36.5 52,5 45 138 250 192 44.5 55 49.2 130 138 134 46.5 46 49.4 190 216 200 47 71 52.3 184 228 201 44 51 47.6 196 215 207 1820 1190 1160 2400 1090 1900 2240 2380 2020 2740 1960 2680 1780 1260 2300 2320 2040 2180 2400 2340 2340 2260 2160 1440 2460 2420 2100 2440 2610 3600 3100 3100 2490 3010* 4000 2700 2760 3940 2960 3100 2920 2600 2600 3020 2680 3400 2700 3120 2740 3420 3200 2600 ' 2620 2800 2820 3710 2510 2540 2202 2653 1561 2455 2910 2565 2404 3185 2565 2883 2556 2349 2497 2708 2419 2550 2535 2561 2500 2627 2583 2240 2540 2583 2520 2932 o o tsS tnet L-J tel 3 n > tr* ('......... . C0?;rt'.n-,n _ TABLE X continued isas22ls;*`v; 0i''''r <n jb .eXcaS^.1^Mot c^rT? TB?T MATERIAL* Vinyl Chloride. (Ethylene herivedl ' ouisiana Chronic Vapor inhalation Toxicity Study - Albino Bata, Albino Mice and Golden Hamsters Chamber Concentration Data Day Number Chacnber Concentration (ppm) Exposure TB-I TB-I1 VB-lil HumberRangeMeanRange MeanRange Mean 24a 274 275 281 304 312 315 316 310 323 332 333 339 345 351 359 360 170 196 197 201 210 224 225 226 228 231 238 *2 39 243 247 251 257 240 47.5 44.5 30 40 45. 5 39 37 46 46.5 49.4 56 47.5 40 49.5 35 46 44.5 52.5 51 56 56 50.5 46 52.5 55 52.0 62 56 55 60 51 45.5 71 59.1 51.2 47.9 49.5 47.5 40.2 42.0 44.2 54 49.2 54,9 56 51.3 48.9 50 39.5 56 50.? 106 108 174 168 202 148 146 182 170 176 206 185 104 190 180 202 170 222 216 242 232 226 172 196 220 280 250 208 210 234 210 246 208 216 203 202 198 204 218 160 174 208 192 208 208 202 203 203 ?28 206 202$ 2420 2180 2160 2360 2140 1800 1800 2080 1960 2160 2260 2160 2380 2140 2640 2420 j 2t 360 2700 2580 2640 2740 2460 2040 2440 2500 2380 2840 2380 2600 2960 2620 2900 2560 2840 2573 2377 2386 2 515 2288 1920 2073 2473 2022 2475 2300 2556 2607 2525 2783 248? 2602 T-IV** Range Mean > * Previously reported by IBT as 1970 -2770, with a mean of 2474 **Gniy gross inspections lyere made of the TE-XV concentrations but the same problems existed with this concentration as with the others. CflA, II 1019 TABLE IX IBT No. 663-03222 VINYL CHLORIDE RATS - SURVIVAL T-IIX - 2,000 ppm Month 5 Report Printout 6 Report Printout 7 Report Printout* 3 Report Printout 9 Report Printout 10 Report Printout 11 Report Printout 12 Report Printout 13 Report Printout** Male 97 96 95 96 94 89 or 92 90 89 84 82 76 74 66 62 42 37 27 23 Female 96 95 96 94 95 92 92 90 85 81 72 68 48 46 34 15(7) 17 5(7) * Non* of #'s or weights agree with report ** All over the lot, 5 body weights shown CONFIDENTIAL !' SuSTect to Protective urn U on.. Conoco, Inc^lo. SO 14th Judicial District $ Calcasieu Parish. LnlaUM . - CM* 01102