Document KJXkOp4R4ZQVvZNkdN8QJEyyo

Oct 02 02 03:2Sp Ralph Cooper 919 541-5138 p.2 A ff M $6 _ H8 ft* S*> USE* UNITED STATES ENVIRONMENTAL PROTECTION AGENCY National Health and Environmental Effects Research Laboratory Reproductive Toxicology Division Research Triangle Park, NC 27711 October 2, 2002 OFFICE OF RliSUARCH AND DEVELOPMENT MEMORANDUM SUBJECT: Control v$. PFOA (30mg/kg) and vaginal cycling, ho FROM: Ralph L. Cooper, Endocrinology Branch (MD-72) RTD, NHEERL, ORD ^ on TO: Jennifer Seed, RAD, OPPT, OPPTS ~ * This is in response to you question concerning the vaginal cycles displayed by F1 females treated with APFO vs. control. In the F1 generation animals, the vaginal smear pattern present In the control and "high dose" APFO (30 mg/kg) dosed groups were evaluated for cyclicity during a 21 day observation period prior to mating. There were thirty animals per group. The cycles were evaluated as having either regular 4-5 day cycles (R), uneven cycling (IR) (defined as brief periods with irregular pattern) or periods of prolonged dlestrus (defined as 4-6 day diestrus periods) extended estrus (defined as 3 or 4 days of cornlfied smears), possibly pseudopregnant, (PSP, defined as 6-greater days of leukocytes) or persistent estrus (PE, defined as 5-or greater days of cornified smears). These data are summarized in table 1, The two groups were not different in any of the parameters measured. Table 1 summarized the data Ovarian Pattern R Ri *' 41 <i PSP PE Type Irregularity Total 3 day Estrus 4 day diestrus no pattern Control APFO (30mg/kg) 18 20 10 9 21 22 66 1 11 CONTAIN NO CP rcCzo=3> C<rD> CD CO o ! uct U ua u a : 2 3 p Ralph Cooper 913 541-513B P Finally, the number of cycles in which a two-day estrus pattern did appear to be greater in the dosed vs control animals. However, as this pattern can be observed in control animals and the occurrence of this smear pattern is not unusual. Thus, it is concluded that the vaginal cycles in the dosed rats was unaffected by APFO. C."' i'O CoD cn ro CD A