Document K6rJ59VxodNVDBr0N3JoDV2mr

A P 3 -2 6 -O IH Study Title Static Acute Toxicity of FX 3820 to the Daphnid, Daphnia aacna Authors Timothy J. Vard Robert L. Boeri BEST COPY AVAii I. GOOD LABORATORY PRACTICE STATEHEST This study was conducted according to OECD Good Laboratory Practice Regulations. Neither the Study Director nor the sponsor are aware of any circumstances that would affect the integrity of this study. Tirtjthy J. Hard Author and Study Director Robert L. Boeri Coauthor 3-n i\ W AVAILABLE C j / lA L iu 7 l'1 o ^ u r 2 l - V Jeanne P. Hagafzuj Aquatic Toxicologist Aquatic Toxicologist EnviroSystems Study Number 90171-3 Page 2 of 17 Resource Afotysta. Inc. Subsidiary of KfeUPOQE II. QALITT A S S O R A K K STITDOPTT Submitted by: EnviroSystems Division Resource Analysts, Incorporated P.0. Box 2130 One Lafayette Road Hampton, Hew Hampshire 03842 Certification: Data presented in this report were derived by methods and with materials identified in the section of the report entitled "Methods and Materials." The test was performed in accordance with EnviroSystems Protocol 90171-3 and the Product Registration Aquatic Toxicology Laboratory Standard Operating Procedures Manual. The toxicity test was performed by Ellen Stanford, Jeanne Magazu, Peter Kowalski, Robert Boeri, and Timothy Hard. The original raw data and final report will be archived at Resource Analysts, Inc. for at least 10 years. All data transcribed from the raw data to the report were checked for accuracy, and all data were verified by Quality Assurance Auditors. Quality assurance audits were performed on BEST COPY AVAILABLE Audit Date 11/12/90 12/20/90 2/ 8/91 3/28/91 Reported to Study Director 11/14/90 12/20/90 2/ 8/91 3/28/91 Reported to Management 3/28/91 3/28/91 3/28/91 3/28/91 Quality Assurance Representative EnviroSystems Study Number 90171-3 Page 3 of 17 BEST COPY MAILABLE m. tabus or cornons SECTION: I. Good Laboratory Practice Statement II. Quality Assurance Statement III. Table of Contents IV. Index of Tables V. Summary VI. Introduction VII. Methods and Materials Vili. Results IX. References Appendix A. Water Quality Data fro Toxicity Test Page 2 3 4 5 6 7 7 11 14 15 Rescue Analyst* Inc. S u b sa a y c i MWJPORE 001610 IT. IKOEZ or TABLES Tahble 1. Tanble 2. Cbeaical characterization of a representative sanple of natural veil water used as dilution water for toxicity test Survival data froa toxicity test Tahble 3. Median effective concentrations (EC50s) froa toxicity test Tabble A.l. Conductivity, pH, teaperature, and dissolved oxygen concentration aeasured during toxicity test Page 8 12 13 16 1ST COPY AVAILABLE EauiriroSysteas Study Nuaber 90171-3 Pagge 5 of 17 Resource Analysts, me. SubsKftory d MRJLJPORE 001611 Y. SUMMARY The acute toxicity of FM 3820 to the daphnid, Daphnia aqua, is described in this final report. The test was conducted for 3m Company for 88 hours during December 18 to 20, 1990, at the EnviroSystems Division of Resource Analysts, Inc. in Hampton, New Hampshire. It was conducted by Ellen Stanford, Jeanne Magazu, Peter Kowalski, Robert Boeri, and Timothy Yard according to the protocol developed for EnviroSystems Study Number 90171-3. The test was performed under static conditions with five concentrations of test substance and a dilution water control at a temperature of 20 1C. The dilution water was filtered natural well water collected from wells at Hampton, New Hampshire. Aeration was not employed to maintain dissolved oxygen concentrations above an acceptable level. Nominal concentrations of test substance were: 0 mg/L (control), 25 ng/I,, 40 mg/L, 60 mg/L, 100 mg/L, and 150 mg/L. Nominal concentrations were used for all calculations. Daphnids used in the test were produced from an in-house culture and were less than 24 hours old at the start of the test. After 48 hours of exposure the control daphnids had an average wet weight (blotted dry) of 0.0001 g, resulting in a loading rate of 0.002 g/L. All daphnids were in good condition at the beginning of the study. Exposure of daphnids to the test substance resulted in a 48 hour median effective concentration (EC50) of 49 mg/L FM 3820, with a 95% confidence interval of 43 - 56 mg/L. WMWws.'. BEST COPY AVAILABLE EnviroSystems Study Number 90171-3 Page 6 of 17 Pesource Anatyifs. m e. Sub 3o y of MWJPOGG 001612 vi. introdoctccb This study was sponsored by 3H Company, St. Paul, Minnesota. The objective of the study was. to.determine the acute toxicity of FM 3820 to the daphnid, a freshwater invertebrate. The report contains sections that describe the sethods and naterials employed in the study, and the results of the investigation. The report also contains an appendix that presents the water quality data collected during the test. TEST SUBSTANCE: VII. METHODS U D MlTDtllLS FM 3820 (EnviroSystens Sasple Husher 2696E) was delivered to EnviroSystess on November 15, 1990. It was contained in a 250 sL plastic bottle that was labelled with the following information: "H 2854-8, FM 3820". The test substance was supplied by 3M Company, St. Paul, Minnesota., Prior to use it was stored in the dark at room temperature. A reserve sasple will be archived at EnviroSystess for a minimum of 10 years. DILUTION WATER: Water used for acclimation of test organisms and for all toxicity testing was collected from wells at EnviroSystess in Hampton, New Hampshire. Water was stored 500-gallon polyethylene tanks where it was aerated. Results of chemical analysis of a representative sample of water are presented in Table 1. TEST ORGANISM: Juvenile daphnids employed as test organisms were from a single source and were identified using an appropriate taxonomic key. Daphnids used in the test were produced from an in-house culture and were less than 24 hours old at the start of the test. Control daphnids were weighed at the conclusion of the toxicity test. Prior to testing, daphnids were maintained in 100% dilution water under static conditions in 4 liter glass jars. During the 14 day acclimation daphnid cultures were not treated for disease and they were free of apparent sickness, injuries, and abnormalities at the beginning of the test. During the acclimation period 24 hours prior to the test initiation the temperature was 19.0 - 19.8C. Daphnids were fed yeast/trout chow and/or the freshwater alga Selenastrum canricornutum daily before the test. EnviroSystess Study Number 90171-3 Page 7 of 17 Rescued Anotysfe In c. Sudsdcvy of MUJPOfiE 001G13 Table 1: Cbeaical characterization of a representative sanple of natural veil water used a dilation water for toxicity test Parameter Unit of Heasureaent Reporting Limit Neasured Value pH Conductivity Organochlorine pesticides Organophosphorus pesticides Polychlorinated biphenyls pH units uahos/ca ug/h ug/L ug/L -- -- 2 5.0 0.6 7.8 1200 NO NO ND Notes: 1. ND * Not detected above the reporting limit. 2. The pH and conductivity values were detenined in a sanple of dilution water collected fron a control test vessel at the beginning of the toxicity test. Additional chemical paraneters were neasured in dilution water during October, 1990 as part of routine biannual water quality analysis. BEST COPY AVAILABLE EnviroSysteas Study Nuaber 90171-3 Page 8 of 17 RucxjcAratysH In c. S u n d a y d M U JPO fii 001G14 BEST COPY AVAILABLE TOXICITY TESTING: X screening test with the test substance was conducted during December 5 to 7, 1990. Nominal concentrations of test substance were: 0.1, 1.0, 10, 100, and 1,000 ng/Ii. After 48 hours of exposure there was 0% survival at 1,000 eg/li, 60% survival at 100 mg/L, and 90% or greater survival at all other concentrations. The definitive toxicity test was performed during December 18 to 20, 1990, according to EnviroSystems Test Protocol 90171-3 (Static, Acute Toxicity Test Vith TH 3820 And The Daphnid, Daphnia maana). which was signed by the Study Director on November 16, 1990. It is based on procedures of the OECD (1984). The test was conducted at a target temperature of 20 1C with five concentrations of test substance and a dilution water control. A 1,000 ag/L stock solution was prepared by combining 1.0 g of test substance with dilution water and adjusting the final concentration to 1,000 ml without the use of a solvent. Nominal concentrations of the test material were: 0 mg/L (control), 25 mg/L, 40 mg/L, 60 mg/L, 100 mg/L. and 150 mg/L 171 3820. Twenty daphnids were randomly and equally distributed among four replicates of each treatment. The test was performed in 250 ml glass beakers that contained 200 ml of test solution (water depth was approximately 6 cm). Test vessels were randomly arranged in an incubator during the 48 hour test (a random numbers table was used to select the location of each vessel). A 16 hour light and 8 hour dark photoperiod was automatically maintained with cool-white fluorescent lights that provided a light intensity of 23 uEs^m'1. Aeration was not required to maintain dissolved oxygen concentrations above acceptable levels. Daphnids were not fed during the test. The number of surviving organisms and the occurrence of sublethal effects and immobilization (inability to swim within 15 seconds after gentle agitation) or other sublethal effects (loss of equilibrium, erratic swimming, loss of reflex, excitability, discoloration, or change in behavior) were determined visually and recorded initially and after 24 and 48 hours. Dead test organisms were removed when first observed. Dissolved oxygen (YSI Model 57 meter; instrument number PRL-3), pH (Beckman model pHI 12 meter; instrument number PRL-4), conductivity (Labcomp SCT meter, instrument number PRL-9), and temperature (ASTM mercury thermometer) were measured and recorded daily in each test chamber that contained live animals. EnviroSystems Study Number 90171-3 Page 9 of 17 Resonce Anat/sts. me. Sub s*/ d MUJPORE 001615 v STATISTICAL METHODS: Results of the toxicity test ere interpreted by standard statistical techniques (Stephan, 1983). The nominal concentration of test substance and the nunber of organisms killed or immobilized were used by the author to calculate the EC50 values. ST COPY AVAILABLE EnviroSystens Study Humber 90171-3 Page 10 of 17 Resource Analysts. Inc. SuOscSary of MUJPORE 001616 Till. RESULTS All test vessels appeared clear initially and remained free of insoluble material throughout the test. Biological and mater quality data generated by the acute toxicity test are presented in Table 2 and Appendix A, respectively, ninety five percent survival occurred in the control exposure. Control daphnids had an average met meight (blotted dry) of 0.0001 g at the end of the test. Loading rate during the toxicity test mas approximately 0.0025 g/L. Exposure of daphnids to the reference toxicant sodium dodecyl sulfate resulted in a 48 hour median lethal concentration (LC50) of 15.6 mg/L. The 24 and 48 hour EC50s for daphnids exposed to TK 3820 are presented in Table 3. Exposure of daphnids to the test substance resulted in a 48 hour EC50 of 49 mg/L FH 3820, mith a 95% confidence interval of 43 - 56 mg/L. Table 2. Survival data fro* toxicity test Noninal Concentration (*g/L) rep. Number Alive 0 hr 24 hr 4S hr Number Affected Ohr 24hr 48hr 0 (control) 1 2 3 4 5 54 555 555 555 000 000 000 000 25 1 555 0 00 2 555 000 3 555 002 4 555 000 40 1 5 5 3 0 0 0 2 555 000 3 553 001 4 544 000 60 1 5 5 2 0 0 2 2 554 000 3 554 004 4 5 55 003 100 1 5 5 0 0 0 _ 2 5 50 0 03 550 0 04 5 50 0 0- 150 1 5 3 0 0 0 2 5 50 0 13 540 004 540 00 ' Note: Affected daphnids vere immobilized (unable to swim vithin 15 seconds after gentle agitation) SnviroSystems Study Nu*ber 90171-3 Page 12 of 17 Resource Anotysh. In c .S u tM d o v 0* MUJPORE 001618 Table 3. Median effective concentrations (EC50s) fron toxicity test Exposure period EC50 95 percent confidence limits Calculation method 24 hours 48 hours >150 mg/L 49 mg/L 43 - 56 mg/L . Probit - W Y AVAILABLE EnviroSystens Study Number 90171-3 Page 13 of 17 Resource Analyst! m e, Subsidiary of MBJUPORE 001619 IX. OECD. 1981. Decision of the Council Concerning the Hutnal Acceptance of Data in the Assessment of Chemicals. Annex 2. OECD Principles of Good Laboratory Practice. C (81) 30 (Pinal). OECD. 1984. Guidelines for Testing of Chemicals. Section 2: Effects on Biotic Systems. Method 202, Daphnid sp.. Acute Immobilization Test and Keproduction Test. Adopted April 4, 1984. Stephan, C.E. 1983. Computer program for calculation of LC50 values. Personal communication. I i ' i I I i i 'i * k BEST COPY AVAILABLE EnviroSystems Study Number 90171-3 Page 14 of 17 t Resxnce Analysis. In c . Subadkxy of MUJPORE 001620 f Appendix A WATER QUALITY DATA FRO TOXICITY TEST BEST COPY AVAILABLE EnviroSyateas Study Nuaber 90171-3 Page 15 of 17 Resouce ^afysts. Inc. S u tn id a y of M U P O R E 001621 Table A.l. Continued. Honinal concentration (ng/L) Rep. Temperature (C) _______________________ 0 24 48 hr hr hr 0 (control) 25 40 60 100 150 1 2 3 4 1 2 3 4 1 2 3 4 1 2 3 4 1 2 3 4 1 2 3 4 20.8 20.8 20.9 20.9 20.8 20.8 20.7 20.7 20.9 20.8 20.9 20.9 20.7 20.7 20.9 20.8 20.8 20.7 20.8 20.8 20.9 20.7 20.8 20.8 20.9 21.0 20.9 20.9 20.8 20.9 20.8 20.9 20.9 20.8 20.7 20.8 20.8 20.8 20.9 20.8 20.9 20.8 20.8 20.9 20.8 20.9 20.9 20.9 21.0 21.0 20.8 20.9 20.9 21.0 21.0 21.0 20.8 20.6 20.6 20.9 20.9 20.9 20.9 20.9 21.0 20.9 20.9 21.0 20.9 20.9 20.9 20.9 Dir'alred Oxygen (ng/L) _______________________ 0 24 48 hr hr hr 8.3 8.3 8.3 8.3 8.2 8.3 8.3 8.3 8.2 8.2 8.3 8.3 8.3 8.2 8.2 8.3 8.2 3.3 8.2 8.2 8.3 8.2 8.2 8.2 8.2 8.2 8.3 8.2 8.2 8.2 8.1 8.1 8.2 8.2 8.3 8.2 8.1 8.1 8.2 8.3 8.1 8.1 8.3 8.2 8.1 8.1 8.2 8.2 8.5 8.5 8.5 8.5 8.5 8.5 8.5 8.5 8.4 8.3 8.5 8.5 8.0 8.3 8.3 8.4 8.5 8.4 8.3 8.4 8.5 8.5 8.4 8.5 EnviroSystens Study Nunber 90171-3 Page 17 of 17 1 S T COP Resouce Analysis. Inc. SJB & Sa y of MAU~3 fiE 001623