Document G5ax57xaRaKk7LMkJRNoxrLbn
AttX-03%3
ACUTE TOXICITY TO FISH TEST SUBSTANCE Identity: athPoceeinadrfse,luic1Poo,Fr1soD,ad2Sfe,l2u,c,oaF3rn,Co3e--,s,14u2a,4l0mf,o,5mno,5aor,tn36eiM,,u6am,7Sm,as7mma,8oitp,,n8leiC,u9AmJ,29S9,s1#0a05lt6,;-171m09.,a01(1y60---aD4l2se-oc7a)bneesreufleforrneicd
bRuetyml acreklslo:sToelvset s(aapmpprolexiims aatemlyix2tu5r%e otef sthtesutebssttasnucbes,ta3n7c.5e%in bwuatytel r and tcNLeeoosltltoc1ssa6uol4cbl.vuseltaatanioncndes3inw7.et5hr%ee tmwesaatdtesera)tm.opAaldlelj.uvasTtleufseotsrstrhaeempoaprclteetudwaalrescloafrntoecmeton3ttrMhaitsipomrnoidoxutfuctrhteieo.n
METHOD
YTGSSMyepuLepaePptehrcp::oicleidNoeSs:mro:tAaPpOtqiilcmEeutaCaeectDpdiuch:t2aeR10le9e3s9s2eparorcmheOlarsganisms, Hampton, New Hampshire AtTSLELPeoeentrxmaeanspatpttdgoilrsyefeitsinhttrsauiiagcchtartm:aauenal0lredepg.mmn0eewo9teor::neitnogNlhyiNidgotof.oio:hsdtrnTht9sni:ene6:o/sg-tPAAhLet:rvvodsopeeuubHorrrbaaist,tshggadteeaenirsnawlstechloyeenalsigvngciesthoh".djtnu=c=ovex2e0ny6n.gt1.irl5ea4ent"mig.oamnn.dwacsonndout cmtieviatysuarnedd.
Test coDnilduittiioonnsw: ater: Aerated, filtered natural well water
DilutioChpnHaowr:ndadntueecrstsciv:hietym: istry
(from671.46000a
mregp/LreassenCtaaCtiv0e3sample): pmhos/cm
Swteoreckparenpdarteedstbsyotlruatniosfnerprirnegpaapraptrioopnr:iaTtheewteeisgthctsonocfeFnCtr-a1t2io0ns
0 0 0 .1.20
wsSsdCbENNNouiaetxrouuulabseurpmnmmeesbctocitdtbiobbalsileynineeentuoniy,rtrrtcnrotiewaerooooatltoafflffvhiytnftictrteeiaoehescotslnrhtelphnesessedlceaasitecpeettdrlmpesaesacnot:sptrthhnetstlrrcaed2isase-nhm,0:atprgeine1mtbollwmoii8merntcpetaorasicalsrctoeme:ti.agnne.:f.Tleli:avpOAedstseeelsnolsrcntcpcltluaeeecleuqtsoanistuonrtavnactcheresoinrsan:noestcuNrcegaegoalostnhnittotiotvcrnanaueooistntnticoeitwtnohadnegneinorteri1snfot5tguplhLdreFeyoCp.tfea-ts1eret2sd0t
Water Dchisesmolivsetrdy odxuyrignegnthraensgteud(0y:- 96 hours):
RESULTS
TpHesrtatne7765gm....e0087p(e----0r8888a-....0562tu9mmr6((e1cggh:o5//oLLnmMutr(r(ogces1ol/a)5Lnentxmertpoxegoplm/seoLpuxseerupexrroa)epst)ouusrreeu)rset)ated as 23 + 2C
Nominal concentrations: Bk control, 2.5, 4.0, 6.0, 10, 15mg/L
Element value:
24-hour 749286---hhhooouuurrr
LLLLCCCC55550000
= ===
>15 mg/L 4>1.181(5(94m..70g--/L152.7.5)m) mg/gL/L
All element values based on nominal concentrations
Statistical Evaluation of Mortality: cPoronbfiidteanncaelylsimisitws.as used to calculate LC50 values and the corresponding
Cumulative percent mortality:
Nominal Test Cone., 24-hours
mg/L
Neg. Control 2.5 4.0 6.0 10 15
0 0 0 0 0 0
48-hours
0 0 0 0 0 20
72-hours
0 0 0 0 40 85
96-hours
0 10 25 85 85 100
0 0 0 .1.21
CONCLUSIONS 4T.h8emtegs/tLswaimthpale9956%-hcoounrfLidCe5n0cfoerifnatethrevaaldomf 4in.0no-w5w.7ams gd/eLt.ermined to be awTneadstetinrnog(t-2wd5iar%escctsloyunbtdojeutcchtteemdsauotbenjreitahclte).m"aTashte-esroiavllad.l"umesixrtuerpeorotef tdheapspulbyjteoctthmaat tmeriixatlurine SStu.bPmauitlt,eMr:inn3eMsoCtao,m5p5a1n3y3, Environmental Laboratory, P.O. Box 33331, DATA QUALITY atRenesatlliyastobicliualittliyod:nastKaalifnmodrisddceehtteerarrmmnkiinninaagttiioo2nn. ooTffhtthhiseesstteausmdtyps,luewbhpsiutlaernitwyc.eellcBocenocncaedunustcertaeatdiom,nliaxinctuktrshee wtinoaxtshicetiteymsotiexfdttu,hreceafclrueooumrloduchshtaebvmeeicetaanklhecanonmwcephdoennoerinnrtte.esrtprircetteindgbtihoelogreicsaulltus.ptaTkhee asnodlv/eonr t REFERENCES TInhcisorsptourdaytewda, sHacomnpdtuocnt,eNdebwy HEanmvirposShyirseteomnsbDehivaislfioonf tRhees3oMurCceomAnpaanlyys.ts, OTHER Last changed: 5/18/00
000.122
Study Title Static Acute Toxicity of FC-120 to the
Fathead Minnow, Pimephales promelas
Authors Timothy J. Ward Robert L. Boeri
Study Completed
March 1992
Performing! Laboratory EnviroSystems Division Resource Analysts, Incorporated
One Lafayette Road Hampton, New Hampshire 03842
Page 1 of 14
000123
I. SUMMARY
The acute toxicity of FC-120 to the fathead minnow, Pxmaphalaa
promelas, is described in this final report. The test was conducted
for 3M Company for 96 hours during February 27 to March 2, 1992, at the
EnviroSystems Division of Resource Analysts, Inc. in Hampton, New
Hampshire.
It was conducted by Jeanne Magazu, Peter Kowalski, Ellen
Stanford, Robert Boeri, and Timothy Hard.
The test was performed under static conditions with five concentrations of test substance and a dilution water control at a mean temperature of 23 2C. The dilution water was filtered natural well water collected at Hampton, New Hampshire. Aeration was not employed to maintain dissolved oxygen concentrations above an acceptable level. Nominal concentrations of FC-120 were: 0 mg/L (control), 2.5 mg/L, 4.0 mg/L, 6.0 mg/L, 10.0 rog/L, and 15.0 mg/L. Nominal concentrations were used for all calculations.
Fish used in the test were purchased from a commercial supplier
(Aquatic Research Organisms, Hampton, New Hampshire) and acclimated
under test conditions for more than 15 days.
After 96 hours of
exposure the control fish had an average total length of 26.5 mm and an
average wet weight (blotted dry) of 0.14 g, resulting in a loading rate
of approximately 0.09 g/L. All fish were in good condition at the
beginning of the study.
Exposure of fathead minnows to the test substance resulted in a
96 hour LC50 of 4.8 mg/L FC-120, with a 95% confidence interval of 4.0
to 5.7 mg/L. One of the eight surviving fish exposed to 15.0 mg/L at
48 hours and one of the three surviving fish exposed to 15.0 mg/L at
72 hours exhibited erratic swimming. The three surviving fish exposed
to 10.0 mg/L at 96 hours exhibited a loss of equilibrium and erratic
swimming.
No other sublethal effects were observed in any test vessel
during the 96 hour exposure.
Page 2 of 14
OOOI24
III. INDEX OF TABLES
Table 1.
Chemical characterization of a representative sample of natural well water used as dilution water for toxicity test
Table 2. Survival data from toxicity test
Table 3. Median lethal concentrations (LC50s) from toxicity test
Table A. 1. Conductivity, pH, temperature, and dissolved oxygen concentration measured during toxicity test
PAGE 6
9 10 13
44I
Page 4 of 14
000125
IV. INTRODUCTION
This study was sponsored by 3M Company, St. Paul, Minnesota. The objective of the study was to determine the acute toxicity of FC-120 to the fathead minnow, a freshwater fish. The report contains sections that describe the methods and materials employed in the study, and the results of the investigation. The report also contains an appendix that presents the water quality data collected during the test.
V. METHODS AND MATERIALS
TEST SUBSTANCE:
FC-120 (EnviroSystems Sample Number 5103E) was delivered to EnviroSystem9 on November 13, 1991. It was contained in a 250 ml plastic bottle that was labelled with the following information: "J2904-I, FC-120, Lot 164, 2-Butoxy Ethanol". FC-120 (a light brown liquid) was supplied by 3M Company, 935 Bush Avenue, St. Paul, Minnesota. Prior to use the test material was stored in the dark at room temperature. A reserve sample (approximately 1.3 grams) will be retained at EnviroSystems for a minimum of 10 years.
DILUTION WATER:
Water used for acclimation of test organisms and for all toxicity
testing was collectedfrom wells at EnviroSystems in Hampton, New
Hampshire. Water was stored in 500-gallon polyethylene tanks, where it
was aerated.
Results of chemical analysis of a representative sample of
water are presented in Table 1.
TEST ORGANISM:
Juvenile fathead minnows employed as test organisms were from a single source and were identified using an appropriate taxonomic key. They were purchased from a commercial supplier (the Aquatic Research Organisms Division of Resource Analysts, Inc., Hampton, New Hampshire) and acclimated at the EnviroSystems facility for more than 15 days. Control fish were weighed at the conclusion of the toxicity test. Prior to testing, fish were maintained in lt)0% dilution water under flow through conditions in an all glass aquarium. During acclimation fish were not treated for disease and they were free of apparent sickness, injuries, and abnormalities at the beginning of the test. During the acclimation period 14 days prior to the test initiation the temperature ranged from 21.1 to 22.5C, and the dissolved oxygen concentration was maintained above 8.0 mg/L. Fish were fed a commercial fish food (EnviroSystems lot number TM03) once or twice daily before the test.
Page 5 of 14
0 0 f> l2 6
Table 1.
Chemical characterization of a representative sample of natural well water used as dilution water for toxicity test
Parameter
pH
Conductivity
Hardness
Organochlorine pesticides
Organophosphoru s pesticides
Polychlorinated biphenyls
Unit of Measurement
Reporting Limit
pH units
--
umhos/cm
--
mg/L as CaCO^
--
ug/L
0.5
ug/L
0.5
ug/L
0.5
Measuri Value 7.0 640 160 ND
ND
ND
Notes: 1. ND not detected at or above the reporting limit. 2. pH, hardness, and conductivity were measured in dilution water collected from the control test vessel prior to
4introduction of organisms and pesticide and PCB data is
collected during routine biannual water quality testing.
Page 6 of 14
iz y
TOXICITY TESTING:
A screening test with the test substance was conducted during January 13 to 17, 1992. The test was conducted at 1, 10, 100, and 1.000 mg/L. After 96 hours there was 0% survival at 100 and 1,000 mg/L and 100% survival at all lower tested concentrations.
A definitive toxicity test was attempted during February 20 to 24, 1992. Nominal concentrations of the test material were: 0 mg/L, 15 mg/L, 25 mg/L, 40 mg/L 60 mg/L, and 100 mg/L. The test was repeated because less than 50% survival occurred at all 5 concentrations after 96 hours.
The definitive toxicity test was performed during February 27 to March 2, 1992 according to procedures of the OECD (1984). The test was conducted at a target temperature of 23 2C with five concentrations of test Bubstance and a dilution water control. No stock solution was prepared and test material was added directly to dilution water contained in the test vessels without the use of a solvent. Nominal concentrations of the test material were: 0 mg/L (control), 2.5 mg/L, 4.0 mg/L, 6.0 mg/L, 10.0 mg/L, and 15.0 mg/L.
Twenty fish were equally distributed among two replicates of
each treatment.
The test was performed in 20 liter glass aquaria
(approximately 20 cm in width, 40 cm in length, and 25 cm in height)
that contained 15 liters of test solution (water depth was
approximately 18 cm). Test vessels were randomly arranged in an
incubator during the 96 hour test (a random numbers table was used to
select the location of each vessel). A 16 hour light and 8 hour dark
photoperiod was automatically maintained with cool-white fluorescent
lights.
Aeration was not employed to maintain dissolved oxygen
concentrations above acceptable levels. Fish were not fed during the
test.
The number of surviving organisms and the occurrence of
sublethal effects (loss of equilibrium, erratic swimming, loss of
reflex, excitability, discoloration, or change in behavior) were
determined visually and recorded initially and after 24, 48, 72, and 96
hours.
Dead test organisms were removed when first observed.
Dissolved oxygen (YSI Model 57 meter), pH (Beckman model pHI 12 meter),
conductivity (Cole Parmer model number 1481-60), and temperature (ASTM
mercury thermometer) were measured and recorded daily in each test
chamber that contained live animals.
STATISTICAL METHODS:
Results of the toxicity test were interpreted by standard statistical techniques (Stephan, 1983). The 24, 48, 72, and 96 hour LC50 values were calculated by the binomial, moving average, or probit method, when warranted. All calculations were performed by the author using nominal concentrations of the test substance.
Page 7 of 14
0 0 o l2 S
V I . RESULTS
All teat vessels containing FC-120 were initially clear and
remained clear throughout the test. Biological and water quality data
generated by the acute toxicity test are presented in Table 2 and
Appendix A, respectively. One hundred percent survival occurred in the
control exposure. Control fish had an average total length of 26.5 mm,
and an average wet weight (blotted dry) of 0.14 g at the end of the
test.
Loading rate during the toxicity test was approximately
0.09 g/L.
The 24, 48, 72, and 96 hour LC50s for fish exposed to FC-120 are
presented in Table 3. The 96 hour LC50 value (and associated 95%
confidence limits) is 4.8 mg/L FC-120 (4.0 - 5.7 mg/L). One of the
eight surviving fish exposed to 15.0 mg/L at 48 hours and one of the
three surviving fish exposed to 15.0 mg/L at 72 hours exhibited erratic
swimming.
The three surviving fish exposed to 10.0 mg/L at 96 hours
exhibited a loss of equilibrium and erratic swimming.
No other
sublethal effects were observed in any test vessel during the 96 hour
exposure.
4
Page 8 of 14
000129
Tibie 2. Survival data from toxicity test
Nominal (mg/L)
rep.
Number Alive Ohr 24hr 48hr 72hr 96hr
Number Affected Ohr 24hr 48hr 72hr 96hi
0 (control) i 2
2.5 1 2
4.0 1 2
6.0 1 2
10.Q .......
1 2
15.0
1 2
10 10 10 10 10 10 10 10 10 10
10 10 10 10 10 10 10 10 10 8
10 10 10 10 5 10 10 10 10 10
10 10 10 10 10 10 10 10
3 0
10 10 10 6 2 10 10 10 6 1
10 10 8 2 0 10 10 8 1 0
00000 000 0 0
000 00 000 00
00000 000 00
0 00 0 0 000 00
000 0 2 000 0 1
000 100 10-
Note: All affected fish exhibited erratic swimming and affected fish at 96 hours also exhibited a loss of equilibrium.
1
Page 9 of 14
000130
Table 3. Median lethal concentrations {LC50s) from toxicity test
Exposure period
24 hours 48 hours 72 hours 96 hours
LC50
95 percent
LC50
confidence limit calculation method
>15.0 mg/L >15.0 mg/L
11.0 mg/L 4.8 mg/L
--
-- 9.7 - 12.5 mg/L 4.0 - 5.7 mg/L
--
-- Probit Probit
* 1
Page 10 of 14
000131
VII. REFERENCES
OECD.
1984. Guidelines for Testing of Chemicals. Section 2: Effects on
Biotic Systems. Method 203, Fish Acute Toxicity Test. Adopted
April 4, 1984.
Stephan, C.E. 1983. Computer Program for the Calculation of LC50 Values. U.S. EPA. Duluth, MN. Personal Communication.
i 1
Page 11 of 14
00O132
Appendix A. WATER QUALITY DATA FROM TOXICITY TEST
<I
Page 12 of 14
000133
Table A.l. Conductivity, pH, temperature, and dissolved oxygen concentration measured during toxicity test
Nominal
concentration
(mg/L)
Rep.
Conductivity (umho/cm) ----------------------0 24 48 72 96 hr hr hr hr hr
0 (control) 1 2
2.5 1 2
4.0 1 2
6.0 1 2
10.0
1 2
15.0
1 2
640 640 650 650 670 640 660 670 660 690
650 670 670 660 660 650 670 670 660 670
660 670 670 660 670 660 670 670 660 670
660 660 670 670 670 660 660 670 660 670
660 670 670 670 660 660 670 670 670 660
670 670 670 670 670 670 670 670 670 670
pH --------------
0 24 48 72 96 hr hr hr hr hr
7.0 7.8 7.3 8.0 8.1 7.0 7.8 7.3 8.0 8.2
7.0 7.9 7.3 8.0 8.2 7.0 7.9 7.3 8.0 8.2
7.0 7.9 7.3 7.9 8.2 7.0 7.9 7.3 7.9 8.1
7.0 7.9 7.3 7.9 8.1 7.0 7.9 7.3 7.9 8.1
7.0 7.9 7.3 7.9 8.0 7.0 7.9 7.3 7.9 8. 1
7.0 7.9 7.3 7.8 8.0 7.0 7.9 7.3 7.8 8.0
i. J
Page 13 of 14
000134
S' '*
n'`
Table A.l. Continued
Nominal
concentration
(mg/L)
Rep.
Temperature (C) ----------------------0 24 48 72 96 hr hr hr hr hr
0 (control) 1 2
2.5 1 2
4.0 1 2
6.0 1 2
10.0
1 2
15.0
1 2
21.5 23.0 21.5 21.5 22.5 21.6 21.0 21.8 21.4 22.9
21.5 22.0 21.4 21.4 21.5 21.5 22.9 21.5 21.3 22.2
21.4 22.5 21.6 21.5 22.5 21.5 23.0 21.5 21.3 22.6
21.5 22.3 21.7 21.2 21.8 21.5 22.3 21.4 21.5 22.3
21.5 22.4 21.5 21.2 22.3 21.5 22.3 21.7 21.4 22.1
21.5 22.7 21.6 21.2 22.2 21.5 22.9 21.8 21.3 22.1
Dissolved Oxygen (mg/L) ------------------------
0 24 48 72 96 hr hr hr hr hr
8.5 8.5 7.7 7.4 6.8 8.5 8.5 7.7 7.3 7.5
8.6 7.9 7.6 7.4 7.0 8.7 8.0 7.5 7.3 7.0
8.6 7.5 7.6 7.2 6.7 8.6 7.6 7.4 7.0 6.0
8.6 7.5 7.5 7.1 6.4 8.6 7.5 7.3 7.1 5.6
8.6 7.6 7.3 6.9 5.8 8.6 7.5 7.4 6.8 6.2
8.6 7.6 7.2 6.8 5.7 8.6 7.6 7.3 7.0 6.0
i
Page 14 of 14
000135
