Document ExXy5rR10vdj9z2ynjJZKMVRb

PUBLISHED REFERENCES TO THE BIOLOGICAL EFFECTS OF VINYL CHLORIDE INCLUDING CHEMICAL ABSTRACTS 1962-JANUARY 14, 1974 AND BIOLOGICAL ABSTRACTS 1962-1973 Assembled In chronological order by: THE DOW CHEMICAL COMPANY January, 1974 Although, this compilation contains most of the references to the biological effects of vinyl chloride as related to mammalian toxicity, the compilation should not be considered as exhaustive. AP00008631 THE TOXICITY OF VINYL CHLORIDE VAPOR A Brief Summary of the Published Literature February 7, 1974 The following articles and abstracts, compiled by The Dow Chemical Company, are believed to include most of the significant scientific information on the toxicology of vinyl chloride in man and animals. The articles and abstracts are arranged in chronological order. Articles published in the last half of 1973 may not be included if they have not been abstracted by Chemical Abstracts or Biological Abstracts. Important review articles which summarize much of the old literature have been used to cover the literature prior to 1961-1962. Therefore, copies of certain articles and abstracts described in these early reviews are not included in this compilation. Since vinyl chloride (chloroethene, CHj^CHCl) is a gas at room temperature and pressure, the common route o toxic exposure is by inhalation. As with many liquified gases, contact of the skin or eyes with escaping compressed vinyl chloride can produce freezing and frostbite. (Torkelson et al, 1961). Vinyl chloride has long been considered to be very low in toxicity by acute inhalation. Lehmann and Flury (1938) summarized the literature and reported work by schauman who considered vinyl chloride to be a candidate surgical anesthetic. Schauman reported little pathological changes even after repeated exposure to anesthetic concentrations. AP00008632 2 W. F. von Oettingen (1955) of the U. S. Public Health Service summarized the available toxicity data on many halogenated hydrocarbons in an excellent monograph. According to von Oettingen, several authors had reported vinyl chloride to be rapidly absorbed and excreted through the lungs and to cause anesthesia. The following is von Oettingen*s summary of the toxicity of vinyl chloride, in animals and man: "As to the toxicity of vinyl chloride, Patty, Yant, and Waite (1930) studied this in guinea pigs. They found that exposure to 20 to 40 vol. percent kills the animals in a very short time, that concentrations of 10 vol. percent are dangerous to life with exposures for 30 to 60 minutes, and that 0.5 vol. percent is the maximum allowable concen tration for several hours* exposure without causing acute disturbances of severe nature. Animals exposed in this way showed some edema of the lungs and hyperemia of liver and kidneys. They considered vinyl chloride less harmful than chloroform or carbon tetrachloride and of a similar order of toxicity as ethyl chloride. Schaumann (1938) found that mice and rats tolerate repeated light narcosis for 4 hours daily on 5 to 8 consecutive days and for 1 hour daily for 4 weeks without showing kidney or liver injuries. Dogs which had been narcotized for 3 hours with 10 vol, percent on 7 occasions in the course of several weeks showed no consider able changes in kidney and liver. Higher concentrations (20 vol. percent) caused in dogs marked salivation, respiratory arrest, and vomiting after narcosis. Peoples and Leake (1933) determined the lethal range for mice with 10 minutes* exposure as 10 to 12 raM per liter, and Schaumann (1934) determined the vinyl chloride level in the blood at the time of cardiac arrest as <40 mg percent and at the time of respiratory arrest as 27 to 30 mg percent, the same value for chloroform being 60 to 70 mg percent. AP00008633 3 Regarding the toxicity of vinyl chloride fov man, Patty, Yant, and Waite (1930) expressed the opinion that the gas does not possess adequate warning properties because of its odor or irritant action, but that it gives warning by producing symptoms of dizziness and disorientation in advance of harm, except when present in exceedingly high concentrations which would cause almost immediately help lessness and unconsciousness. Inhalation of 2.5 percent will soon cause dizziness, disorientation, and a burning . sensation in the soles of the feet, which symptoms disappear immediately after discontinuation of the exposure except for a slight headache which may last 30 minutes. These authors suggested the use of vinyl chloride for narcosis in man.M As a result of two deaths in Canada, the acute inhalation toxicity of vinyl chloride was studied by Mastromatteo et al (1960) who reported that exposure of mice, rats, and guinea pigs to 10, 20, and 30 volume percent vinyl chloride caused the following mortality: NUMBER OF DEATHS IN DIFFERENT GROUPS OF FIVE MICE, RATS AND GUINEA PIGS EXPOSED FOR THIRTY MINUTES TO VARYING CONCENTRA TIONS OF VINYL CHLORIDE IN AIR Vinyl chloride concentration (percent by volume in air) 10 20 30 40 Mice Laboratory animal Rats Guinea pigs 0/5 1/5 5/5 0/5 0/5 5/5 0/5 0/5 1/5* 2/5* Total 0/15 1/15 11/15 2/5 Total 6/15 5/15 3/20 14/50 * A delayed death occurred within 24 hours following exposure. AP6b608634 4 Some pulmanary hyperemia and engorgement was observed by these investigators, but liver and kidney injury were remarkably low. Deaths were due to narcosis. The first report of studies to determine the effect of long-term repeated exposure (6 months) were summarized by Torkelson, Oyen and Rowe (1961) as follows: "Repeated exposures of laboratory animals to several concen trations of vinyl chloride in air were conducted to determine the chronic toxicity of this material towards animals in order to assess the hazard to humans. Vinyl chloride was found to have a slight capacity to cause liver and kidney injury on repeated exposures. Male and female rats showed micropathological changes after repeated daily 7-hour exposures at 500 ppm for 4.5 months. Repeated 7-hour exposures at 200 ppm for six months resulted in micropathological changes in the livers of rabbits and statistically significant increases in the average weight of the livers of male and female rats but no detectable changes in dogs and guinea pigs. Repeated 7-hour exposures at 100 ppm resulted in slight increases in the average weight of rat livers, the other species were not affected. All species studied tolerated repeated daily 7-hour exposures to 50 ppm for six months with no detectable injury. Repeated daily 1-hour exposures at 200 and 100 ppm of vinyl chloride were without effect, longer exposures caused a slight increase in liver weight. AP00008635 5 The standard for evaluating regular daily 7 to 8-hour exposures may be defined as the concentration below which practically all analytical results must fall. The value of 100 ppm is suggested as this standard for vinyl chloride, with a timeweighted average for all exposures not to exceed 50 ppm." Lester, Greenberg, and Adams (1963) took strong exception, to the conclusion of Torkelson et al (1961) that 50 ppm should be a maximum time weighted average exposure for workers. On the basis of 3 months exposure of rats to 2 volume percent and 19 days to 5 volume percent, they concluded that 500 ppm was acceptable as a TLV despite minor changes which they observed in rat livers and which they considered "were within the normal range and were not pathologic in nature." Because of these conflicting reports, the TLV Committee of the ACGIH in 1963 chose to change their TLV from 500 ppm as a maximum time-weighted average for industrial exposure to 500 ppm as a ceiling value. (American Conference Governmental industrial Hygiene, 19631 * Since 1962, numerous European articles on vinyl chloride have appeared, particularly in. the Russian literature. For several reasons, these articles are difficult in interpret in terms of chronic toxicity since the reported exposures were to mixtures or were otherwise ill-defined* the exposures are often acute* or the criteria of effect was a minimal change in response or behavior, such as a subtle change in a conditioned response. AP00008636 6 in 1967, reports appeared in the literature describing a condition known as acroosteolysis in workmen engaged in polymerization of vinyl chloride to polyvinyl chloride. Harris and Adams (1967) reported on two cases in Europe. Wilson et al (1967), reported on 37 cases in the B. F. Good rich Company. Basalaev (1970) described the use of large frame photofluorography for diagnosis of acroosteoLysis in Russia. Basalaev et al (1972) described studies in rats and rabbits and claimed to have produced the disease in these species, Fethiere and Kerzner (1972) discussed the disease as did Juhe et al of the University of Bonn (1973). Jtihe et al (1973) described a syndrome consisting of (arranged in decreasing order of occurrence) thrombopenia, splenomegaly, liver damage, obstruction of ventillation, circulatory obstruction, and skin and bone alteration. As a result of this problem, the University of Michigan in 1967 was retained by the Manufacturing Chemists Association to investigate this disease in sponsoring American companies. The results of a large scale epidemiological study of workers then currently employed in vinyl chloride and polyvinyl chloride production were reported in three publications by this group (Dinman et al, (1971) Cook et al, (1971) and Dodson et al,(1971)). Dinman et al summarized the study as follows: "An epidemiological study was performed covering 5,011 employees with 21,510 man-years experience in various phases of vinyl chloride (VC) and polyvinyl chloride (PVC) manu; facturing in 32 plants throughout the United States and Canada. The total number of definitive cases of acroosteolysis AP0b608637 7 (AOL) was 25? 16 other individuals were under suspicion. This condition is clearly associated with the hand cleaning of polymerizers. Workers engaged in other phases of VC or PVC manufacturing do not appear to be at risk of developing AOL. The importance of Raynaud's phenomenon as a con comitant of AOL is emphasized. Several statistical approaches for rapid medical survey are suggested. Acroosteolysis appears to be a systemic rather than local disease. Presentlyr neither the etiological agent nor its portal of entry is known." Cook et al describes the polyvinyl chloride production process in considerable detail. They concluded that although no etiological agent could be identified, "There appeared to be a correlation between, the extent of degassing prior to entry into the reactor" and the incidence of acroosteolysis. Mutchler and Kramer presented a paper at the 1968 Gordon Research Conference which was subsequently published (1972), which reported on "The Correlation of Clinical and Environ mental Measurements for Workers Exposed to Vinyl Chloride " The authors drew the following conclusion* "Our findings suggest that repeated exposure to vinyl chloride at twa levels of 300 ppm or above for a working lifetime together with a very low level of vinylidene chloride may result in slight changes in certain physiologic and clinical laboratory parameters. The possibility of some impairment in liver function tests must be considered, even though no overt clinical disease was evident in any of the individuals studied* We shall continue our study, but suggest that similar studies to help clarify the effects of this material be performed for other worker populations exposed to vinyl chloride alone." AP00008638 3 The MCA sponsored studies by Dinman et al and Mutchler and Kramer appear to be the only two significant eqidemiological studies on vinyl chloride reported in the literature. P. L. Viola, in an attempt to produce acroosteolysis in animals/ exposed rats 4 hours per day, 5 days per week to 30,000 ppm (3%) vinyl chloride vapor. (Viola, March, 1970). In his first report on the results of 12 months exposure, he described metaplastic changes in the bones which he con sidered similar to the human disease acroosteolysis. He made no mention of having observed cancer in these animals until the Tenth International Cancer Congress in May, 1970. In the abstracts of this meeting, (Viola, 1970), and sub sequently in May, 1971, Viola, Bigotti and Caputo (1971) reported tumors of the skin, lungs and bones occurring first after 10 months of exposure. The authors summarized this work as follows: "Rats (Ar/IRE Wistar strain) exposed for 12 months to vapors of vinyl chloride developed tumors of the skin, lungs, and bones. The cutaneous tumors, which always appeared in the area in which submaxillary and parotid glands are located, have been histologically recognized as epidermoid carcinomas, papillomas, and mucoepidermoid carcinomas. The morphological characteristics of lung tumors, which occurred in a lower percentage, were mainly of the adenocarcinoma type, with the exception of a single epidermoid tumor originating from the epithelial covering cells. In a minor number of rats, a large proliferation of cartilaginous tissue diagnosed as osteochondroma developed in the metacarpal and metatarsal regions of the four limbs. AP00008639 9 This report by Viola et al is apparently the earliest and only publication in which carcinogenic activity has been ascribed to vinyl chloride. Although there are possible deficiencies in Viola's study, such as his very impure sample, the presence of food and bedding in the exposure chamber, excessive exposure concentration as well as in the statistical evaluation and interpretation of the lesions, the report is of serious concern and has resulted in additional animal and epidemiological studies which are currently underway (MCA Press Releases), i AP00008640 Lehman, K. B., and F. Flury. Toxicology and hygiene of industrial solvents. 1938. As translated by Eleanor King and Henry F. Smyth, Jr. 1941 Von Oettingen, W. F., M.D. The halogenated aliphatic, olefinic, cyclic, aromatic, and aliphatic-aromatic hydrocarbons including the halogenated insecticides, their toxicity and potential dangers. Public Health Service Publication No. 414, U. S. Department of Health, Education, and Welfare, Washington, D. C. 1955. Mastromatteo, E., M.D., A. M. Fisher, H. Christie, and H. Danziger. Acute inhalation toxicity of vinyl chloride to laboratory animals. American Industrial Hygiene. Association Journal, Volume 21, No. 5, October, 1960. Torkelson, T. R., F. Oyen, and V. K. Rowe. The toxicity of vinyl chloride as determined by repeated exposure of . laboratory animals. American Industrial Hygiene Association Journal,: Volume 22, No. 5, pp.. 354-361. 1961. Gabor, S.., Lecca-Radu, and I. (Manta; Certain biochemical indexes of the blood in workers exposed to toxic substances (benzene, chlorobenzene, vinyl chloride). From.`/Toksikol. i Klinika Prof. Zabolevanii Khim. Etiol. Sb. 221-223. 1962. Irish, D. d. Vinyl chloride, CH^=CHC1 (Monochloroethene). Industrial. Hygiene, and Toxicoldigy, Volume 2, pp. 1303-1305. F. A. Patty, Editor. 1962. Lester, D., L. A. Greenberg, and W. R. Adams. Effects of single and repeated exposures of humans and rats to vinyl chloride. American Industrial Hygiene Association Journal, pp. 265-275, May-June. 1963. Gabor*: S., M. Radu, N. Preda, S. Abrudean, L. Ivanof, Z. Anea, and C. Valaezkay. Inst. Hyg. Cluj., Romania. Bucharest 13(5), 409-418. 1964. Hardie, D. W. F. Chlorocarbons and chlorohydrocarbons: vinyl chloride. Kirk-Othmer Encycl. Chem. Technol., 2nd Ed. 5, 171-178. 1964. Kuebler, H. The physiological properties of aerosol propellants. Aerosol Age 9(4), 44, 47-48, 50, 90-91. 1964. AP00008641 Mamedov, M. Vinyl chloride. Baku: Azerb. Gos. Izd. pp. 127. 1964. Grigorescu, I. and G. Toba. Vinyl chloride; industrial toxicologic aspects. Rev. Chim. 17 (8):499-501. 1966. Harris, D. K. and W. G. F. Adams. Acroosteolysis occurring in men engaged in' the polymerization of vinyl chloride. Brit, Med. Journal, 5567, pp. 712-714. Ulus. 1967. Wilson, R. H., W. E. McCormick, C. F. Tatum, and J. L. Creech. Occupational Acroosteolysis, report of 31 cases. The Journal of the American Medical Association, Volume 201, No. 8, pp. 577-581. 1967. Vazin, A. N., and E. I. Plokhova. Pathogenic effect of chronic exposure to vinyl chloride on rabbits. Farmakol Toksikol, 31(3):369-372. 1968. Vazin, A. N., and E. I. Plokhova. Pathogenesis of a disease, developing during chronic action of vinyl chloride on the organism. Farmakol Toksikol 31 (3):369-372. 1968. Vazin, A. N., and E. I. Plokhova. Creation of an experi mental model of "toxic angioneurosis" developing from the chronic action of vinyl chloride vapors on an organism. GIG TR Prof Zabol 12(7):47-49. 1968. Antonyuzhenko, V. A. Occupational poisoning by vinyl chloride. GIG TR Prof Zabol 12(3):50-52. 1969. Baretta, E. D., R. D. Stewart, and J. E. Mutchler. Monitoring Exposures to vinyl chloride vapor: breath analysis and continuous air sampling. American Industrial Hygiene Association Journal, Volume 30, pp. 537-544. 1969. Vazin, A. N., and E. I. PLokhova. Dynamic changes in epinephrine-like substances in rabbit blood following chronic exposure to vinyl chloride fumes. GIG TR Prof Zabol 13(6):46-47. 1969. Clapp, J. J., C. M. Kaye, and L. Young. Metabolism of allyl compounds in the rat. Biochem. Journal 114(1), pp. 6-7. 1969. Vazin, A. N., E. I. Plokhova. Changes in adrenaline-like substances in rabbit blood following chronic exposure to vinyl chloride fumes. Gig. Tr. Prof Zabol. 13(6), pp. 46-47. 1969. AP00008642 Vasin, A. N., E. I. Plokhova. Changes in the cardiac activity of rats chronically exposed to vinyl chloride vapors. Farmakol Toksikol, 32 (2):220-222. 1969. Schottek, Wolfgang. Toxicology of vinyl chloride. Chem. Tech., 21(11), pp. 708-711. 1969. Smirnova, N. A., and 13. P. Granik. Long-term side effects of acute occupational poisoning by certain hydrocarbons and their derivatives, GIG TR Prof Zabol 14(5)*50-51. 1970. Basalaev, A. V. Experience with the use of large-frame photofluorography in examining skeletal bones of persons occupationally dealing with unsaturated hydrocarbons of the ethylene series (olefins) and their chlorine deriva tives {vinyl chloride, trichloroethylene). GIG TR Prof Zabol 14(11):34-37. 1970. Kudryavtseva, 0. F. Characteristics of electrocardio graphic changes in patients with vinyl chloride poisoning. GIG TR Prof Zabol 14(8)=54-56. 1970. Viola, P. L. Pathology of vinyl chloride. Medicina del Lavoro, Volume 61, No. 3. March, 1970. Translated from the Italian. 1970. Viola, P. L. Cancerogenic effect of vinyl chloride. Presented at the Tenth Int. Cancer Cong., Houston, Texas. Regina Elena Inst, for Cancer Research, May 22--29. 1970. Dinman, B. D., W. A. Cook, W. M. Whitehouse, H. J. Magnuson, and T. Ditcheck. Occupational Acroosteolysis: 1. An Epidemiological Study. Archives of Environmental Health, Volume 22, pp. 61-73, January. 1971. Cook, W. A., P. M. Giever, B. D. Dinman, and H. J. Magnuson. Occupational Acroosteolysis: II. An Industrial Hygiene Study. Archives of Environmental Health, Volume 22, pp. 74-82. January. 1971. Dodson, V. N., B. D. Dinman, W. M. Whitehouse,A. N. M. Nasr, and H. J. Magnuson. Occupational Acroosteolysis: IIT. A clinical study. Archives of Environmental Health, Volume 22, pp. 83-91, January. .1971. Viola, P. L., A. Bigotti, and A. Caputo. Oncogenic response of rat skin, lungs, and bones to vinyl chloride. Cancer Research, Volume 31, pp. 516-522, May. 1971. AP00008643 Vinyl chloride. Documentation of the Threshold Limit Value American Conference of Governmental Industrial Hygienists/Third Edition, 1971. Kramer, C. G., and J. E. Mutchler. The correlation of clinical and environmental measurements for workers exposed to vinyl chloride. American Industrial Hygiene Association Journal, Volume 33(l):19-30. 1971. Meyerson, L. B. and G, C. Meier. Cutaneous lesions in acroosteolysis. Arch Dermatol 106 (2):224-227. 1972. Jiihe, S., and C. E. Lange. Long-term exposure to vinyl chloride. Dt. roed. Wschr. 97*1972. Basalaev, A. V., A. N. Vazin and A. G. Kochetkov. Pathogenesis of changes developing due to long-term exposure to the effect of vinyl chloride. GIG TR Prof Zabol 16 (2):24-27 1972. Markowitz, S. S., C. J. McDonald, W. Fethiere and M. S. Kerzner. Occupational acroosteolysis. Arch Dermatol 106(2):219-223. 1972. Jiihe, S., C. E. Lange, G. Stein, and G. Veltman. Uber die sogenannte Vinylchlorid-Kranklieit. Dtsch. roed. wschr. 98, pp. 2034-2037. (German). 1073. Jiihe, S., C. E. Lange, G. Stein, and G. Veltman. On the so-called vinyl chloride disease, Deutsche Medizinische Wochenschrift, 98_(43), pp. 2034-2337. English translation. 1973 Levina, M. M., V. S. Filatova, E. G. Belaya, S. A. Ashirova, Y. P. Tikhomirov, A. L. Levin, R. Z. Mostinskaya, and B. E. Kuznetskii. Hygienic certification of the building projects development, and reconstruction of plants pro ducing organochlorine products. Gig. Sanit (4):86-87, 1973. Filatova, V. S., V. K. Vinogradova, and L. S. Bashkirova. Hygienic evaluation of working conditions in some chemical plants operating in the open air. Gig. Tr. Prof Zabol (8):8-11. 1973. AP00008644 7039 Chemical Abstracts 7040 VL 65, I960 33--ALIPHATIC COMPOUNDS LAVUKCX I. nttMOH AMD HAJt&T It. YALB Chlorocarboas and chlorohydrocarbons: chlorinated pinflu, Chloroearboat and cblorohydrocarbons: allyl chloride, b. h D< W. F. Hardic. Kirk-Otkmer Encytl. Chen. Tecknol., 2nd Ed. _ Pilors. Kirk-Oihmer Encycl. Chem. Tecknol.. 2nd Ed. 5, 20.Vlr 3, 231-40(1964XEng). A review on chlorinated derive, of (1964XEng). A review on the phys. ana chem. properties natural or synthetic higher paraffins, without regard to whether (typical addns. to the double bond, simple replacements of Cl the parent material it normally solid or liquid, including proper formation of atlyi esters and of N coinpda., and synthesis of ties, manuf., economic aspects, handling, storage, and uses. 39 complex mols.), manuf. (mechanism, reaction variables, corn references. VNJZ practice, feed prepn., reaction system, and product recovery i Automatic preparative gas chromatography apparatus. Ya. -- safety and handling (toxicity, precautionary measures, ami M. Kucbcrov and A. P. Lizogub. Ntfieptrerobotka i Ntflt- remedial treatment), and uses. 25 references. VNJZ kkim., Akad, Ucvk Vkr. SSR, Rtsp. Metmdomsto. 5b. 1965, Chlorocarbons and chlorohydrocarbont: athyl chloride 135~40(Ruh). A 1:1:0.5 isooctane-octane-nonane mixt. was D. W. F. Hardie. Kirk-Otkmer Encycl. Chem. Tecfutol., 2nd FA. sepd. in a column of 6 m. length and 16 mm. diam., N carrier gas 5, 140-7(1964XEng). A review on the phys. and chem. proper* ejodty 0.6 l./min., Wheatstone bridge current 170 ma., re- ties, manuf., economic aspects, standards, handling, toxicitv" corder sensitivity 50 mv. Samples (3.5 cc.) were injected at & and uses. 39 references. VVNNJtyZ 112*, andthe components were collected and frozen in glass tubes Chlorocarbons and chlorohydrocaxbons: diehioroethylenas. at 0*. The yield of the components was 66-60%, although by D. W. F. Hardie. Kirk-Otkmer Encycl. Chem. Tecknol., 2nd Ed condensation of the vapors, the yield could be increased to 80%; 3, 178-83(1964XBng). A review on the phys. and chem. prop the purity of the collected compels, was 99.9%. BNJR erties, manuf., and uses of 1,1- and 1,2-dichIoroethylene. m yaaettnne of active nitrogen with organic substrates. IV. _ references. VNJZ Isoprane. TeruUyo Hanafusa and Norman N. Lichtin (Boston Tri- and perehloroethylene. S- A. Miller. Ckem. Pretest Umr., Boston, Mass.). Can. J. Ckem. 44(10), 1230-2(1966) Eng. 47(6), 288-75(1966XEng). A review of proecsses for the (Beg); cf. CA $5, 17622c; 64, 110704. The reaction of isoprene with active N at 80*/2 mm. was investigated. Products trapped at -196* were HCN, MeCHXHj, CHrCHi, CiH,, manuf. of CtHCU and CiCU. The relative feasibilities depend on the availability of raw materials and the utilisation of by. products. 18 references. Paul A. Haas HCiCH, propyne, EtMeC:CH,, (CH,:CH)t, and propane, o Chlorocarbons and chlorohydracarbons: chloroacetylenes. Monomeric products trapped at --78* but volatile at 60*/0.01 D. W. F. Hardie. Kirk-Otkmer Encycl. Ckem. Tecknol., 2nd Ed. ma. were 0-methylpyrrole, pyrrole, NH, 1-cyanoisoprene, and 3, 203-6(1964XEng). A review on the properties of mono- anil at least 12 other unidentified compds. The polymeric product dichloroacetylene. 6 references. VNJZ waa a complex mixt. which probably contained CN and amino Chlorocarbons and chlorohydxocvbons: chloroprene. p. s, groups. C. M. Buess Bauchwitz(B. I. du Pont de Nemours & Co., lac., Wilmington, StudyofheteroienecBZoxldationofo-buteneito 14-butadiene --Del.). Kirk-Otkmer Encycl. Chem. Tecknol., 2nd Ed. 5, 215-31 in the presence ofstaam. I. 1. Yukel'son and B. A. Boguslav- (1664XBng). A review on the phys. and chem. properties (in- akil. Tr. Lab, Kkim. Vysokomolehul. Seedirt., Voronethsk. Univ. eluding reactions with O, S, SOt, NiOi, halogens, H, halides, and 1964(3), 63-6(Ru). The effect of steam eonen. on oxidn. of n- hypohalous adds and esters, Dicb-Alder addns, and dimeriza- butenes over newly developed catalysts (oxides of group VIII tions, free-radical reactions, and polymerization), prepn. (from modified by elements of group IV) was studied. The raw mate- . C*Hj via monovinylacetyiene, butadiene, butanes and butenes, rial was an industrial fraction of butenes with the compn. Ht * and 1,3-diehloro-2-butene), industrial manuf., health and safety 0.1, CsH< 0.3, CiH 0.6, C4H111.6, l-C4H1S2.fi, 2-QH| 68.4, and factors, and isomers and homologs. 73 references. VNJZ C*H 1.6mole-%. Oxidn. was effected in a continuous flow app. Chlorocarbons and chlorohydrocarbons: trichloroethylene. at 660* and a l: 2.5 dUn. of the butenes with air over 1 ml. cata- D. W. F. Hardie. Kirk-Otkmer Encycl. Ckem. Tecknol., 2nd Ed. lyst (particle size 2-3 mm.) in a quartz tube (diam. 8 mm.). 5,183-9G(1994XBag). A review on phys. and chem. properties, With an increase in butene-air-H0 mole ratio from 1:1.6 to -- manuf., economic aspects, standards, handling, toxicity,and 1; 15.8, the 14-butadiene (I) content 0/ the contact gas increased uses. 53 refertacts. VNJZ from 13.8 to 20.8 mole-% (basis dry gas). Curves were obtained Chlorocarbons and chlorohydrocarbons: methyl*n* chloride. showing the dependence of total butene conversion and 1 yield D. W. F. Hardie. Kirk-Otkmer Encycl. Chem. Tecknol., 2nd Ed. per amt. of passed and decorapd. butene on the steam concn. in 3, lll-19(1964XEng). Areviewon thephys.andchem.proper- the initial mixt. From Rtf. 2k., Khim. 1966(4), Pt. II, Abstr. ties, manuf., economic aspects, standards and analysis, handling, No. 4N7. MVRK * toxicity, and uses. 40 references. VNJZ Chlorination reaction in the manufacture of hexachloroben- Chlorocarbons and chlorohydrocarbons: chloroform. D. W. xene. Jerzy Jaworski and Tadeusz Mazonski (Katedra Tech- F. Hardie. Kirk-Otkmer Encycl. Chem. Tecknol., 2nd Ed. 5, nol. Chem. Org., Polltech. Siaska, Poland). Prttmysl Ckem. 43 119-27(1964XBng). A review on phys. and chem. properties, (2), 02-H1964XPol). Continuous chlorination of o-dichloro- manuf., economic aspects, standards, analysis, handling, toxic- bensene in toe gas phase (at 306-400*) and in the presence of a __ ityt, and uses. 25 references. VNJZ fixed bed catalyst was investigated. The catalyst used was in Chlorocarbons and chlorohydrocarbons: tetraehloroethylene. expensive activated C, Carbopol 2, in the form of irregular D. W. F. Hardie. Kirk-Otkmer Encycl, Ckem. Tecknol., 2nd granules, about 5 mm. in diam.. with a density of 230 g,/l. and a Ed. 5, 195-2Q3d864XBng). A review on the phys. and ehcra. sp. surface arcs of 157.7 m.`/g. A uniform temp, gradient was properties, manuf., economic aspects, standards, handling, Obtained when the upper part of the vertical pipe reactor was toxicity, and uses. 45 references. VNJZ filled with less active catalyst from a previous charge. The / Chlorocarbons and chlorohydrocarbons: other chloraethones. use of a partly deactivated catalyst resulted in a total absence of D. W. F. Hardie. Kirk-Otkmer Encycl, Ckem. Tecknol., 2nd Ed. catalyst baking, even when its load was increased by 40%. The 5, 148-70(1964XBng). A review on the phys. and chem. prop reaction product contained mere than 65% of hexachlorobenzene, erties, manuf., economic aspects, standards, handling, toxicity,' besides some peatachiorobenzene. A schematic diagram of the and uses of 1,1- and 1,2-dlchloroethane, 1,1,1- and 1,1,2-tri- equipment used is shown. Edward A. Ackermans chloroethaoe, 1,1,1,2- and 1,1,2,S-tetrachloroethanc, pema- Chlorination of isobutylene to methallyl chloride. E. M. ~ chloroethane, and hexachloroethane. 101 references. VNJZ MokrU, Kh. Z. Kotovicb, and D. K. Tolopko. Khim. Prom.. Chlorocarbons and ehlorobydreearbona: carbon tetrachloride. Inform. Ncuh.-Ttkhn. Zb. 1965(4). 7-10(Ukrain). A 99.5% D. W. F. Hardie. Kirk-Otkmer Encycl. Ckem. Tecknol., 2nd Ed. pure isobutylene (I) was prepd. by dehydration of tof-BuOH 5,123-39(1964)(Eng). A review on the phya. and chem. proper on AhOj at 350* with the 2 l./hr. rate of I formation. The ties, manuf., economic aspects, standards and analysis, toxi catalyst was regenerated at 600-20 for 3 hrs. in a stream of hot _ city, and uses. 73 references. VNJZ air. Mixing 14 moles 1 with 1 mole Cl at 90-100* gave a mixt. 9 Synthesis of trlalkyiamine-type extraction agents by the Hof of products, which on rectification at 70-2* gave methallyl mann reaction. B. N. L&skorin, D. I. Skorovarov, and V. A. chloride (II). The fraction collected at 72-5* consisted of a 95:4 Semenov. Zk. Prikl. Kkim. 39(5), 1049-55(1906y kus). ratio of u and dixncthylvinyl chloride which were difficult to Lab.- and bench-scale (--100 kg.) methods were described f>r sep. A lower I-CI molar ratio caused addnl. chlorination of the prepn. of RN, where R Ct-Ci*. RBr was prepd. by products and a higher ratio decreased the yield of II. At 90*, --heating a mixt. of ROH, HBr or NaBr, and H*SO. The nr. a 62-72.6% yield of 11 was obtained. BMJW phase obtained, contg. 76-80% RBr and unreactcd ROH, wa> Chlorocarbons and chlorohydrocarbons: vinyl chloride. treated with 8-34% NH4OH at 100-1808/several atm. Tlu* D. W. F. Hardie. Kirk-Otkmer Encycl. Chem. Teehnol., 2nd Ed. 3, 171--8(1964 X Eng). A review on the phys. and chem, proper ties, manuf., economic aspects, standards, handling, toxicity, yield of R*N increased with decreasing NHj-RBr ratio to 2-3:1 and with increasing temp. The yield was not affected by NHOH concn. or size or R. The yield of resin began to increase ut and uses. 38 references. VNJZ h &180*; the yield of solid product, probably RNBr, tncrcareil Chlorocarbons and chlorohydrocarbons: methyl chloride. with decreasing temp. The storting conditions of nminmioly-'U D. W. F. Hardic. Kirk^)ihmer Encycl. Chem. Terhnol., 2nd Ed, became milder as the batch sin.- increased, because the reaction 1- S, 100-ll(19C4XBnR). A review on the phys. utiii chem. prop exothermic aud a large batch provided a largvr pri>tK>rtioii of tin- erties, manuf., economic aspects, standards, analysis, handling, required heat tliuri a simdl baU'ii under similar condition-,. A toxicity, and uses. 43 references. VNJZ typical final product of a Ct-C prepn. contained RjN U5-7i>, AP00008645 Vul. <12, 15)05 4509 Off -Toxicology and ,1 ir /'dilution 4510 llieiimglubiit 7H1'*; satil. with CO) in 10 ritigx r-Mpirittg O under Alend. Vl,, nriilixhivu). Etidornnato&y 76(1). 151-5(1906) hyiKTlKirie ciiimHUous. With 7'.; CO* present in the supplied O, Ming). Ally! isollii'veyaiiiU,-. snliiini>ured in single doses of the CO wuh eliniiuuUil ucurly twice us fast us when pure O was <5 to 4 mg. <lipm.so.-d significantly the uptake by rut respired. Of 22 patients with coul gas poisoning, the respiration thyroids. The miiiunisinukm of ullyl isolliiocyunutc to rats for if O at 2 uttn. pressure led to rectiverytno fatalitiesJ. All pa- 5(1 days, in doM-s corresponding approx. to thy total content of tients recovered consciousness 30*90 min. after inhalation of 0 mustard nils in cabbage, caused changes in the thyroid similar to under pressure began. Of 70 other severely coul-gassed patients, those following cabbage feeding. RCFN only 3 did not recover. The results demonstrated that hyper- The physiological properties of aerosol propellants. Hans barre O should be administered as soon as possible ill CO poison- Kuebler (Sprulitechuik G.m.b.H., Reinfehlen, Cer.). Aerosol ing. Data are given on 1 patient with barbiturate poisoning who Age 9(4), 44, 47-8, 5(1, U-l(lU(i4KEiig). A discussion of the recovered from respiratory and cardiovascular depression under__physiol, limits for many aerosol propellants was presented. hyperbaric O when other methods failed. Other reports indicate Rats, mice, and guhieu pigs were cxixmed for 2 hrs. daily for 100 that there arc no advantages in giving O at more than 2 atnu. days to 0.5. 1.5, and 5,0'.'] vol. of the propellants, vinyl chloride pressure. At higher pressures, toxic effects of O appear, per- (1), and mists, of triclilorofluoroinethanc (ll) and dichlorodi- haps resulting from the presence of CO in the blood. fluoromethane (III) (50:50 and 10:90). The animals showed no _, . ............................................. W. C. Tobie reactions to the mixts, at any concn. or with pure I to 1.5%, Diagnoiit of salt p<dsung in animals. > M, Boliosiewicx b With 5% 1 thy animals first showed increased mobility which u Obyzsza Szkola Rolmcta, Wroclaw, Roland). Zessyiy Maui. then gradually reduced. Upon autopsy no histol. damage was II yeseej Stholy Rciniczej Wrodaonu, Weterynaria 11(42), 3-43 seen. Mice under a plastic cover were sprayed with a shellac* (1962)(Pol). Administration of high NaCl doses (3-4 g./kg.) based hairspray with I, II, and 1U propellants for 30 see. daily caused acute Intoxication in mice and pigs. NaCl poisoning was for 5 weeks at a distance of 20-25 cm. There were no histol. noted when Ka* values in the brain and liver exceeded 150 mg. changes in the lungs. The thermal decompn. wasdetd.at 1000*. % and those of Cl~ 180 mg. % in brain, 70 mg. % in muscles.-One g. I yielded 39.5 mg. CO, 11 mg. COClt( and 102 mg. HClj and 250 mg. % in liver. The toss of water in brain, muscles, 1 g. 11(1.5,7.0, and94); Ig. 111(1.5,2.4,79); 1 g.sym.diehloroand liver amounted in mice to 3.8, 7.5, and 9.2%, resp. In tetrafiuoroethane (0.8, 12,0, 59); and 1 g. methylene chloride pigs this decrease in brain, muscles, and in liver amounted to (16.2,18.0,81). BKJN 4.5, 9.2, and 8.7%. K. Belsecka Concentration on molecular sieves as prslireiniry determine- Toxicity of the oleander leaves. Symptoms, pathological tios of trace components of mine air. Jerzy Muzyczuk (Giowny anatomical damages, and methods of glucoside identification. c Inst. Gornictwa, Katowice, Poland). Chem. Anal. (Warsaw) C. E. Eliakis, E. C. Eliakis, and A. S, Coutselinis. Ann. 9(4), 671-82(1964). Trace components of mine air, such as Med. Legale Criminal., Police Sci. Toxicol. 4i(July-Aug.), CtHi, C*H, and a-CHi, were coned, on a mol. sieve of 5 A. 367-88(1961 XFr). Decoctions (10:150) of oleander leaves, type at 0 and 30 and retention times were measured. Prod- contg. 3 X 10"* g. of ncriin and 4.5 X 10-< g. of oleandrin, were ucts of desorption at 300* were analysed in a gas chromatograph used. R/ values of 0.65-0.70 for neriin and 0.66 for deendrin -- with a conductometric detector, in some cases addnl. concn. were found by means of paper chromatography (Whatman No. 1, was used. The method makes it possible to increase the sensi- ascending, undimensionaf, 3:1:3 EtOAc-HOAc-HsO). With tivity of the detn. of CjH, C1H4, and CjHi 10-100 times. The the same method, very similar values were found for digitoxin, results of the detn. of traces of gases in a sample of mine air are lanatosidc, and other glueosides. No abortive effect could be given. The effect of concn. can be estd. from the retention vols. detected on pregnant rats. Twenty ml. of decoction (coated. Z. Kurtyka to Vs) resulted in the appearance of toxic symptoms in rats after d Permeability of newborn rat skin to a volatile hydrocarbon, 20-00 min. The oleander components seem to affect the central "C-laheled p-cymens. Guillaume Valette, Yves Cohen, and nervous system, esp. parts related to the telencephalon and Jacques Wepierre (Fac. Pharra.. Paris). Compl. Rend. Sec. erebellum, the symptoms resembling those of deeerebration Biol. 15fi(5), 950-4(1964XFr). The compd. readily diffused paralysis, according to Sherrington, also displaying tendency, to through the hairless skin and was detected in various organs. A total or partial disintegration of cerebellum. In rats, which special app. was used to prevent any inhalation of the vapor which received 3 ml, of decoction intraperitoneally 9 times every other might invalidate the findings. Since the hair follicles had not day, congestion of the parenchymatosie organs took place. yet been formed, they are not Indispeasible for penetration of a Damage to kidneys, liver, Heart, and peribronchial inflammatory volatile hydrocarbon. L. E. Gilson congestion and atelectasis of the lungs was detd. microscopically. Flue gas from plants manufacturing synthetic fatty adds and From CZ 1963(24), 10175. MWCR. alcohols. F. 1. Dubrovskaya (F. F. Erlsman Sci.-Res. Inst. Uso of the chicken embryo in the assay of aflatoxiQ toxicity, e Hyg., Moscow). Gigiena i Sanit. 25(1), 7-1CX1961 XRuss). M. Jacqueline Verrett, Jean Pierre Marliac, and Joseph Me- Results are reported on the investigation of 1252 air samples Laughlin Jr. (Food & Drug Admin., Washington, D.C.). (580 in March/April and 672 in June/July) collected 250-5000 J. Assoc. Offie. Agr. Cfumirtr 47(6), 1003-6(1964). Preliminary m. from factories manufg. fatty acids and fatty ales. In all results of an investigation of the general systemic toxicity of samples in the spring, hydrocarbons (max. 56 mg./m.) were afiatoxin (1) in chicken embryos are reported. The studies are detected; fatty acids, in 54% of the samples (tame max.); and not complete enough to verify if any l produces any specific unsatd. hydrocarbons, In half the samples (max. 13 mg./m.*). pathal. lesion in the embryo. Injections of test solns., in pro- In the summer, 70% of the samples contained hydrocarbons pylene glycol vehicle, were made, before incubation, in fertile (max. 63-90 mg./m.*), 52% contained fatty acids, and 56% White Leghorn eggs either by way of the yolk or air cell. De- contained unsatd. hydrocarbons. Acetone and formaldehyde velopmeat of the embryo was observed for the full 21day In* were found In only a few samples. Prom CZ 1962(47), 17163. cubation. Injection of solos, of pure X Bi and Gi, and of exts. / MRCR of {-producing mold cultures indicated that the chicken embryo ` Maximum permissible concentration of chloroprene In the was sensitive to these compds. The relation of toxicity of the atmosphere. A. V. Mnatsakanyan (N. B. Akopyan Inst, samples to mortality at time of hatching, exhibited a dose re* Epidemiol, and Hyg., Erevan). Gigiena i Sanit. 29(9), 13-18 sponse. Exts. of I-free peanut products were nontoxie to the (1964KRus$). A concn. of 0.22 mg. chloroprene/m.* was the embryo. Adda, of I Bi to such uncantaminsted exts. produced threshold concn. for decreasing the sulfhydryl concn. in brain the expected toxicity. Injection of exts. from contaminated -- homogenates and increasing adcaosinetriphosphatase activity peanut product resulted in a toxic response that correlated well in the livers of rats exposed constantly for 60 days; 0.08 mg. /m*. with that obtained by injection of pure I Bi solns. at same dose is considered a proper max. John Howe Scott levels, and in most instances the cnem. analysis was confirmed. Effect of diemorobutene on the olfactory organ* and ocular The presence of I Gi, B, and Gi had no apparent effect on the snalysors. G. V. Dcroyan (Med. Inst., Erevan). In. Akad. toxicity due to 1 Bi at the levels at which they occurred in the Wank Arm. SSR, Biol. Nauki 17(7), 101-4(1964XRuss). The particular samples tested. Sepn. of 1 Bi from contaminated 0 threshold concn. for a dlchiorobutane (I) effect on the olfactory ext*, by thin-layer chromatography, and its subsequent elution analysors was investigated with 15 patients and was equal to from the plates and injection into the eggs, confirmed that the 0.34 mg./m.* Effect of I on the visual center was examd. on toxieity of these exts. was due primarily to their I B, content. 3 patients. The results indicated that in some cases 0.34 mg./ H. A. Lepper m.* of I produced harmful effects on dark adaptation. A concn. Electrophoretic and chemical studies on serums of swine follow* _ of 0.34 mg./m.* of 1 in air was considered harmful. g the feeding of toxic peanut meal. E. Annau, A. H. Corner, M. Charmandarian j. E. Magwood, and K. Jericho (Animal Diseases Res. Inst., Effects of inorganic fluorides on animals. J. W. Suttic Hull). Can. J. Comp. Med. Vet. Sci. 28(11), 264-8d964XEng), (Univ. of Wisconsin. Madison). J. Air Pollution Control Assoc. The consumption of toxic poamitmcal by swine leads to marked 14(11), 461-4, 480(1904XEng). F" toxicity in cattle can re- alterations in their electrophoretic serum patterns. Expti. car* suit from the ingestion of forage contaminated by industrial rit-d out by ebem. assays, moving boundary electrophoresis, h processes. Cattle arc affected when F" levels in the compact and starch-gel electrophoresis showed a relative decrease in the bone exceed 5500 ppm. Marginal toxic effects arc noted in albumin, or. os*, and 0-ciobulins, while vglobulin appeared to animals with F" concns. in these bones of 4500-5500 ppm. be considerably increased. Rudolph Scidcn The concn, of F- on forage tliut can produce these concns. in Goitrogenic activity of allyl isothiocyanata. A widespread the skeleton depends on a no. of factors. Air quality standards natural mustard oil. Pavel Langer and Viktor Stoic (Ceskoilor based on atm. F~ levels are not feasible for protecting livestock AP00008646 16211 Chemical Abstracts 16212 Vol. 03, 1905 wt. remain* unchanged, the new substance is believed to be the (OHKPOt)i* After activation to CtNKPOt) life of catalyst ci isomer of I. A, Baulins during described dehydrogenation process is compared with Alkali metal .V.N-dialkyldlthiocarbamatas. Stlg Aiwrstrom Ctb(l>0)j/C**(0HXI>04) (A/B) when this value is >2, buta and Anders Uhlin (Uuiv, L'ppxulu, Swetl.). Arkiv Kami 24(64), diene produecd/kg. catalyst is >500. For example, the tabu 503-tU966XEng); cf. CA 61, 7924c. The following data are lated results were obtained. The catalyst is stcamwi to total reported (metal ui BujNCSeM, m.p., no. of associated molecules a in CHCli soln., di-td. cbullinscopicidiy): K, 134.3-6,5, (J; Itb. 148-50', 5; Cs, 158-9*. 4. The distribution coclhchnt (/) of RiKCSjN'a (1) between CHCli-IIjO is siuninurucd its follows (R in I, f): Me, U.V X Id-*; lit. 1K X 10"; l'r, 270 X W; aud Bu. 790 X 10-*. Harry L. Yule _ Mamedov, M.: Vinilkhlorid (Vinyl Chloride). Baku: Azerb. cstaiyst l s 3 -4 5 8 Ca 30.34 38.80 29.80 30.84 38.87 30.03 Ni 5.17 8.14 5.37 5.30 5.30 5.SO rotrt'%rOi c 55.89 1.65 2.59 54.33 50.6* 00.45 l.Wl 1.75 l .50 2.83 3.31 2.40 55.00 1.82 3.24 66.52 1.70 3.31 A_. sh 0.03 0.43 0.40 A/B 3. 18 2. 12 1. 87 1. 87 1. 66 1. 49 Butadiene wiilyii kf./ltg. 844.85 838.62 164.88 176.32 264.83 208.08 Go*. Izri. 1964. 127 pp. activation in closed reactors at 300-400*. After burning the Smith, Petar A. S.: Tito Organic Chemistry of Open-Chain lubricant with 0, and steaming to 565-580*, several routes are Nitrogen Compounds, Vol. I. New York: Benjamin. 1005. described to bring the temp, to operating conditions. For ex- 418 pp. b ample, temp, was kept at 566* for 8 hrs.. increased by 2.8*/8 hrs. to 593*, 2.8*/12 hrs. to 610*, and 2.6*/24 hrs. to complete Purified hydrocarbons. Esso Research and Engineering Co. Noth. Appl. 290,921 (Cl. C 07c), June 25. 1965 Appl. March 29, catalyit 1963; 17 pp. To obtain starting material for isomerization and 1 paraffin alkylation, a mixt. of hydrocarbons should be purified^ 2 from alkenes, aromatic hydrocarbons, and S-contg. compds. Therefore, at first this mist, is heated to 93,3-176.7* and the vapor is brought to an absorption zone contg. a Linde I3X or 10X 3 4 6 6 B.buMsai vol.^vol./ 158.0 156.8 100.9 157.3 179.2 158.1 steam/ temp. n-butcaes feed 18.1 18.8 18.0 18.1 18.0 18.0 083 058* 882* 852* 80S 857* regeneration eteaa air vol./*'0)./hr. 783 145.7 780 132.6 829 148.0 823 147.4 875 149.3 816 146.2 oa- seloe* version Uvitv too1e% 26.7 38.3 36.7 38.5 38.6 36.4 93.4 92.4 92.8 94.0 93.0 92.9 molecular sieve. So only most of the alkenes remained, which production. The process consists in alternative dehydrogenation are removed by condensing the vapor and bnnging the liquid to of steam-diluted n-butenes and steam-air regeneration of catalyst, another Lmde 10X or 13X molecular sieve at 21 .l-6o.6?. c Typical conditions are given in the 2nd taWe? N. Russo Isohezanes by the isomerization of n-hexane. I. A. Balashov 'acetylene* from a mixture of hydrorartwns, mainly and E. A. Timofeeva. U.S.SJL 172,310 (Cl. C 07c), June 29, i101*6,11?;. J5ue1I? A.-G. (by Manfred Reich). 1965. Appl. May 18, 1964. Isobexanes are prepd. by the isora- er. M 96.642 (Cl. C 07r).July ]Lfi. .1965. Appl. July 10, 1963; eriration of *-C,H,, on AlCl, and to increase the yield, 0.5- tjSSi^****- ^.Ger' (SeeFr, 1370.443, CA 62, 1^% ListearateorMVP oil or TsIATIM-201 lubricant is added --144e7f>; According to G. 1,190,456 acetylenes can be re- to the A1CU. From Byvl. Iscbret. i Tovarnykk Znakov 1965(13), from a mixt. with didefins by selective catalytic faydro- MHCL genation in the gas phase._ The catalyst was composed of 5-50% 4-Methylpent-l-ene. Toyo Rayon Co., Ltd. (by Ryoji vl s ^ main group I and II Nakanisht and Aldhisa Miyake). Japan. 20,373('6S), Sept. 10, f **"6" .U^!t in.forTMredu"ble**** UP Appl. March 12. 1962; 2 pp. Dimeriration of propylene in all- d ' 11o!one or more metals phatic hydrocarbon in the presence of a reaction product of K * ^ Zn w or m5^ea4 with ether was described. Thus, a mixt. of 40 cc. heptane, 4 g. Gf ;annbeterCt!?1wt*i The acetyJene* wftre bydro- K, 2.16 g. anisoie, and 0.33 mole propylene is heated^in 100 cc. "**f *2"* 5Sf^ autoclave at 150* for 10 hrs. to give 0.12 mole hexene composed H without reduction of the diolefins. Thus, CuCO,.Cu- of 4-methylpent-l-eue 78, 4-inethylpent-2-ene 16, 1-hexene 5, (?)* NiCO,, and CaCO, we brought onto pumice, fixed by end others 1% Hiroshi Kataoka --water-glass, and reduced at 200 by H. The catalyst thus pro- Isomerizatioa of the C olefinic hydrocarbons. P. E.Tulupov "f and N. G. Polyanskii. U.S.S.R. 172,308 (Cl. C 07c). June 29, as^aLjW!!?-assfSMar s sSnSiussKSSSaSitaEa S^f'.hi'cK i'MSS5SfSf*rSKe SSiSiSi225L23Sl=H!S* "?SL1S?K swelling and the process is carried out at 150. From Byut. of^8`talyst and the rates of the gas phase comp^nentsiu-e given Itobret i Tovarnykk Znakov 1905(13) 17. MHCL Marianne Paulmg Hydrogenation of 1-butene on an irradiated molecular sieve. chlonde. Organization of the State Committee for the James M. Caffrey, Jr. (to Texaco Inc.). U.S. 3.210.437 (Cl. Chemical ludus^under the State Plannmg Committee of the 260-683.9), Oct. 5, 1965, Appl. Oct. 25,1961; 3 pp. A molecu----- Go^cilf .blusters of the U.S.S.R. (by E. G. Bondarenko. lar sieve of the Ca aluminosilicate type, such as Linde 10A, is ex- M. Ktwnwkaya I. L. Boratyrev and S. I. Cherepanov), posed to H for 15 min, to 2 hrs. at 400-550* at subatmospheric 12.2/289 19V C 29' l9?i,Al?P^jSPr?1 *? pressure, then y-irradiated with 0.1 X 10* rs. Contacting H and 1963. BuCt is prepd. from thereactionof BuOH with HCl m the 0.5 to 50 cc. gaseous butene per g. of this adsorbent at 0-100* P*nee of ZnCU at elevated temp. To increase the yield and results in hydrogenation of the butene. L. R. Caswell , ensure a continuous prows, the process is earned <mt m an aq. Olefin dimers Monsanto Co. (by James M. Schuck and / "!?. ZnC1*- From B'/ul- * raniy*A ZnakmlQ6S Robert G. Schults). Fr. 1,403,273 (Cl. C 07e), June 18, 1965; ,. MHCL U^. Appl. July 12, 1903 : 53 pp. Active C is treated with NH, _ Chlorination of alkynols. Chemische Werfce Huels A.-G. and impregnated with a Co salt of an alkanoicacid or an O-contg. ^r- 1,402,396 (Cl. C 07c), June 11, 1965; Ger. Appl. Aug. 3, mineral acid, the products are activated at 200-300* under an in- 1963; 10_pp. Dichloroallcenola are prepd. by chlorination of ert gas to give Co/C. C*_ ^>lt*lins are polymerized in the pres-__alkynols in the presence of HCl at <--35*. Thus, 224 parts ence of the prepared Co/C to give liquid dimers which can be used CKiCCHiOH, ^b. i1n14--'1*5', sa*t4d. at --45* and 9-00 mm. with 22U in the prepn. of biodegradable detergents; catalysts activated at 450-550 can be used to dimerize C>_i alkenes contg. an internal double bond. The olefins arc passed over the Co/C at 0.1-2 g./g. catalyst/hr. at 10-50* and 3.5-21 kg./cni.* Thus, 300 g. parts HCl and treated at the same temp, and pressure with 284 parts Cl yielded 245 parts ClHC:CClCHOH, b* 80-7, and 132 parts CHChCChCHjOH, b, 76-95*. Similarly, CHjOH- CCl:CClCH.OH, b, 136-18, and CH,OHCCUCCI,CH,OH, b, BPL activated C is treated with 300 ml. coned. Ml,, dried 2 hrs, 0 175-85 , are prepd. Basil Farab at 120*, treated with a ln. of 2IM) g. Co(NOj), in 250 ml. 3,3-Dichloro-l,l,2,2-tetr&fluotopr9paiL. Imperial Chemical HjO, dried 3 hrs., heated in vacuo 18 hrs. at IJd*. und activated Industries Ltd. (by Brain G. Hutton and James Raventosi. in vacuo at S274*. A reactor is charged with nig. propylene, Brit. 1,004,606 (CL C 07c). Sept. 15, 1965, Appl. May 15, 1962; 2-4 g. catalyst, and 10 ml. heptane and the ' ixt. is heated to 3 pp. Process for the prepn. of the title compd. (I). Ciat3.9 85* in 1/2-1 hr., heated 5 hrs. at .*<5, aud coo' 1 to room temp. _ l./hr. is bubbled through 225 g. HFjCCT-CHjCl in a flask under to give 13.04 g. product/g. catalyst ;i& cvnifuirv*. with S.02 for the a packed glass column surmounted by a trap cooled with solid Cl i control. BDPF Tlie mixt. was kept at the reflux temp, by irradiating with a Hu Catalytic dehydrogenation of i/-butenes to butadiene on a vapor lump for 7 hrs. 'The product of 4 runs weighed 280 g. und nickel calcium phosphate catalyst. Prediction of catalyst life. was sepd. by gas phase chromatography lu she 103 jf. I. b. 77*. Gondrk'h-C'Ulf Chemical*, hie. (hy William I.. Kvlil und Douuhl of UU.T'.,' purity. A rabbit inhaling 3*7 I in o- for lu nd.i. tlnn S. Msndver). Fr. t.405.509 (Cl. Il n|/, C (i7i, July, 11. i'/t' i fur l hr. WS18 um-sflnlizitl with vry little fall in lili/*l-pres- t'.S. Ajipl. del. 2:>. r.WkJ: 2'J !'|>. A i-.t.!> -1 is prepd. rtuitg. sure and t> carii:i0 irregularities. In (Kitbogeifirev i-m-e. Un 31 wt. `.I C.i, *.! , I'O,, small tjuuiitiliis ralio L.C.^ ACj, fur I is 2.1 that i 7 win-rc A.L',> is 1 a of Cr,(>) :s a pninndor and lubricant. By x-ray analysis of miu- minimum rmu-it. by vol. to fully uucsthclisc 'r>` i of the miiv atm aeliv.iU-il mI.iIv^i with tin- K,* fr.>|iH ttcv nf Cit. the crystalline I..C.w,is the itihiiiuum coitcil. by vul. lo kill ht\\l tf the mice witlur, fuiius *4 Ca salts arc hUuHIu,) as iimi.u l> C.i^ I't),)^ and C.i,- 3D min.>. Cxinp.ii.ihlc i.tiiusarc; ether 1(.M:3.5I, Cl I Cl 2.ifi AP00008647 11231 Chemical Abstracts 11292 VoLi Prom. Tokstkol. i Kiinika Prof. ZaMtmnw Xltm. Eliol. (Mot* and lability of bonds formed by incorporation of methionine am) cow: Got. Isd. Med. Lit.) si- IMS, 163-5. Neotone D (N- cysteine by decreasing cysteine. The mechanism of the studied phenyl-2-aaptittariamine) (I), rmphthol (II), and aniline (HI) had compds. is discussed. Prom Ref. Zk., Kkim. 1964, Abstr. X,, L .D. values nf 8.280, ami 356 mg./kg., reap., for an acute single 01288. MVKK ' intraperitoneal administration in animal expts. Acute poisoning Certain biochemical indexes of the blood in worker* exposed to with 1 and HI resulted In a predominance of kwn of the central tozk substances (benzene, chl&robenienv, vinyl cnloridc. nervous system (side posltinti. continuous spasms, absence of Silvia Gabor, Mina L*eca-Radu,mid I. Manta. Prom. corneal reflexes). The degree of disorder of conditioned reflex i Kiinika Prof. Zabolevattii Kkim. Eiicl. (Moscow: (, {(. activity was mote pronounced in the cue of II. Under the same Med. Lit.) Sb. 1962, 221-3. Oxidative processes and liver func- c..o...n..d..i.t.i.o...n..s..,...I did not have a marked effect ou the central nervous tion were studied in 82 workers with DDT, hexuchlorocyd'*. system. In the case of subacute and esp. chronic poisoning with hexane, and poiy(vinyl chloride) resins who were chronically e\- I, II, and PI a decrease in the hemoglobin content and no. of posed to vapor* of C*H, ehlorobcnxcne (I), and vinyl chloride erythrocytes in the blood, which was moat pronounced in the (II). A decrease in catalase activity and increase in peroxidase ease of U and III, were observed. Phagocytosis decreased and and indophenoloxidase activities, and an increase in the glutu. liver function was disordered. Prom ntf. Zk., Kkim. 1964, thione level In the blood were observed. Catalase and peroxi- Abstr. No. 5X321. MVRK darn were most strongly affected. These changes appeared dur- Tba eeote nazeetic effect of methyl alcohol. T. Miyaoia Jog the first year of Work (for II after 1 year). Random changes (Univ^ToIcyo). Xirskiu ltaku 49(2), 124-7(1962). MeOH(10 in the no. of erythrocytes in workers with CH, a decrease in al oe 20%) was administered orally to unanesthetized and curanted rabbits under artificial respiration, and the else, activity of the central nervous system was recorded from the amygdaloid nu bumins and increase in or and yglobulins, increase in aldolase activity, and decrease in sorbitol dehydrogenase activity were also observed. A study of oxidative processes can give valuable cleus, hippocampus, and frontal cortex. The 1st change in the data for diagnosis of early changes. From Ref.Zk., kkim. 1964, electroencephalogram was slow wave activity In the frontal cor- -- Abstr. No. 61313. MVRK tex. Similar changes appeared in the amygdaloid nucleus and Biogenic amines, biogenic aldehydes, and alcohol. Aaron hippocampus when >8 g./kf. was given orally. These changes Feldstein, Hudson Hoagland, Keith Wong, and Harry Freeman reiemhisrt those seen alter BtOH ingestion; there wss no qual. (Worcester Found, for Exptl. Biol., Shrewsbury, Mass.). differences la narcotic effect between MeOH and EtOH. From Quart. J. Stadia Ale. 25. 218-23(1964). EtOH blocked the EmotM* Med. Sod. II 16(3), Abstr. No. 1629(1993). Pamolodcal-morphological changes of the heart dueTCtoDcNom'. e metabolism of sarotofun-^Cto 5-hydroxyindoleacetic*"Caad (I). Recovery of I, based on urinary MC, was 76%. Two hrs. after biaed administration of Saivarsan and ulfxmine. SusutnuKuno ingestion of 30 cc. of EtOH I recovery was 64%; after 60 cc., (Nippon Uoiv,, Tokyo). Niekidtti Igoku Zasski 18, 1236-46 37%; after 90 cc., 30%. Scrotonin-"C administered simul (I960). An examn. was made of rabbit hearts following com taneously with 60 cc. of EtOH gave 32% of I; 1 hr. after EtOH, b--i-n--e--d---u-s--e---o--f--S-a--i-v--a--r-s-a- -n---a--n--d---s-u--l-f-a--rra-in--e---(-s--u- lfanilajYm'iiVdet:). .T-he_co*t.v* 80%; 2 hrs, alter, 34%; 4 hra. after, 49%; and 24 hrs. after, cotnhant use of both drugs gave rise to myocardial fibrosis in 20% _ 78%. EtOH or AcH blodted monoamine oxidase or aldehyde of the animals. The heart muscle underwent regressive changes dehydrogenase. EtOH intoxication, in part, may be due to such ax fatty or vacuolar degeneration, with no direct connections altered levels of brain biogenic aldehydes, biogenic ales., or bio- to the formation of fibrotie changes. Myocardial fibrosis also genic amines. H. W. Gordon occurred. Rukuson Yoh Biochemical and histological studiet on the liver of rata in early Determination of th* degree of absorption of certain aromatic stages of dimethylaminoaxobenmo feeding. Yoshito Xakajima compounds by workers. Tadeuss Dutkkwicz and J. Piotrow. d (Med. Coll. Sapporo, Japan). Sapporo Irak* Zasski 18(1), ski. Prom. Toktikol. i Kiinika Prof. Zabolewiii. Kkim. Eltoi, 43-64( 1960). Rats were fed a km-protetn diet contg. 0.06% di- (Moscow; Goa. led. Med. Lit.).S&.1963,49-63. Dependencies methylamineaxobenxene (I), and ia early stages of the feeding the wsxe found between the amt. of aniline (I) absorbed by the or- livers were analysed biochem. and histol. There was a decrease anistn and the amt. ofaminophenol, excreted with the urine, and in ribonucleic acid (RNA) and the degree of P* ineorporation'into jetween the amt. of nitrobenzene (H) absorbed and the amt- of__RNA was reduced. There were no changes in the mt. of de- nltrophenol (IQ) excreted with the urine, and between the amt. oxyribonucleic acid (DXA) or in the degree of WP incorparation. of CH absorbed and the amt. of phenol excreted with the urine. There was an increase in anaerobic and aerobic glycolysis and The rate of absorption of l applied to the skin wss 0.2-0.7 mg./ only a slight decrease in endogenous respiration. From Biol. *q. cm.-hr. (increasing with increase in the temp, end moisture Abitr. 30(19), Abstr. Vo. 64464(1961). TCCQ content of the skin): for IX end C,H, this figure was 0.25-3.2 and Cutaneous toxicity evaluation of Air Force development ms- 0.4, reap, (decreasing with increase in skin temp.). In industrial terUH. IV. Morns V. Shelansld and Karl L. Gabriel (tnd. work, 2/3 of the absorbed I penetrates into the organism through Biol. Res. & Testing Labs., Inc., Philadelphia. Pa.). U.S. the skin and 1/3 through the respiratory tract. Special units do- Dept. Com., Office Teeh. Serv., AD 265,486, 8 pp. UOfll). fining the degree of exposure (daily absorbed dose) should be Twelve materials were studied via the prophetic patch test rue- established and the rate of excretion of products of metabolism of thod on lab. animals and human subjects to det. the primary r- toxic compds. should be detd. From Rtf. Zk., Kkim. 196*, ritation effect, fatiguing effect, and sensitization index. Alkyl- Abstr. No. 61318. MVRKsubstituted deoslin was too severe a primary irritant to test on Certain problems In csrbon disulfide toxicology. E. Noferand humans. Diethylcydohexane was a severe primary irritant. T. Wronska-Sspakowa. Prom. Toktikol. i Kiinika Prof. Zs. All other materials were safe to use in contact with the human Meoanii Kkim. Eliot. (Moscow: Go*. Izd. Med. Lit.) Sb. 1962, sklrt. From US.Govt. Res. Rept. 37(1), 76(1962). TCVD 114-19. Exptl. animals which had been chronically exposed to The therapeutic adaptability of experimental Ever cirrhosis is CSt received a injection of "ZnCU intravenously. Th* MZn con- , the rxt. XI. Thloacetamide cirrhosis in the rat and the influence tent in the liver, kidneys, bona, lungs, brain, hesn, muscle*, and of glucocorticoids, androgens, and tolbutamide. P. Knottier, erythrocytes was considerably higher than in the control. The p. Nolte, H. A. Kuehn, and vV. CreutsfeMt (Med. Urtiv. Kiln.. Zn content in the spleen was the same as in the control. Exere- Freiburg, Ger.). Z. Cot. Exptl. Med. 146(5), 486-99(i963)iRc- tion ol Zn with the urioe and fecee wai also subnormal, esp. dur- print]. White rats were given thioacetamide (1) orally (175 ing the first days after poisoning. The activity of alk. phospha- mg./kg.) to cause acute liver damage and 40 mg ./kg. to produce tase in the blood of rats exposed to CSi was 30% lower than in the--chronic liver damage. Glucocorticoids and anabolic steroid* control. Rats exposed chronically to CS, vapor* for 5 months were administered and the effects observed. Prednisolone tul- excreted 18% (av.) more products of nicotinic acid metabolites ministered to the acutely X-trcated animals reduced mortulity than control atiimitis; excretion of JV'-mcthylnicotmamkle in markedly. In chronic! polsoningthcMicleurchimgeainthclivcr creased at the expense of a decrease in excretion of other metabo cells were reversible. In chronic I poisoning higher level* >4 lites. In the liver nicotinic acid and its compds. were reduced glucocorticoids increased mortality. 23 references. Ill, Car- by 26%. The mechanism of the action of CS* is discussed. It is 9 Bon tetrachloride cirrhoeis in tbe rat and the therapeutic influence hypothesized that CS* affect# lipid metabolism. From Rrf.Zh., of glucocorticoids with and without simultaneous admimatra Kkim. 1964, Abstr. No. (112(15. MVRK tion of antibiotic* sad androgens. K. Disrhcr. H. Lmiff. \l\ Meehanism of the toxic action of certain chlorinated bydrocar- Crcutxfeldt. and H. A. Kudin, ibid. 500 10. Livers of white boas us*d la industry. |>. Gurkavi. Prom. Tnksikol. i rat* were cirrhotic after 24 injections of CCI* (0.75 ml./kg.) over KUnika Prof. Zabolevanii Kkim. Eliol. (Moscow: Cos. Izd. Med.__a 12-wcvk period. Subcutaneous injection f prcdniwiUmc or Lit.) Sb. 1962, 119-1)2. In isotope expts. on animals poisoned cortisone prevented cellular regeneration mid connective tissue nth chlurlnutcd hydrocarbons (tctrnehloropropane (I), tetra- formation. Oral administration had no effect. Administration ilaroncntunc (II). tetrachlorolicptatic (III), and other limnologs of outilmitlcH to animals treated with CCI, and ghc<*cortii`oiU of CCM diwirilers in intvrp,fut]tm of labeled amino add* into reduced mortality by reducing infection*. HO reference*. tiKKiie proteins (decrease in inon*trillion nf cysti'ine.*# iutu liver Carol Stull protein and ;ontin globulin*. im-rcasc in inc*riM>ratiois of glycine- It Differentiated determination of silica in the lungs of animals ,46V alight disnrtler in iiiennuir.ilioii of iiH'iliinninf) wore fntuid. N. T. Vurym-Agai'Vti. /(X/uo/'h. VoUr. (ligi.-Hv i Hpidrin The vwritm* chhriiuited eminxl*. had difTmm dhruptive HItvts. Ill incrfu^l inc>riM>nition of cystoim'^A; nil iliv sioiHod nrilino acids were iiieorpornltd into mtuiii iithuaiiii* ul n fastiv rate than thmrtsk. flusrina (-Kiev: Go*. Iwl. Mcl. Lit. I'kr. Will) V. 1963. IK) I. !'`rrii'lK. the pathogcued* id silicons, tin* iviut< nt Ire*' and iMmtid SiOj should lx- detd. scp. A series nf six'll in the eontrols. IU decreased the ratio U-twtrn the xtubility crpts. were cuculuctetl with the lungs of auiinnU dusted with el.tv AP00008648 Q683 Cknttirnl Abstracts 9684 Vol. 152, [ (ifia of quartz have been demonstrated. Modifications of tlic matrix theory muy explain silicosis us it patlml. process resulting from the mol. and structural makeup of silicic ucitl. Over 154 ref* & 1'rcfla. S. Almidcuii. Liana tvimof, Zoe Alien. uml Clara ValaczIray < Inst. 11 y(t-Clitj. Romania). fgtertn ( Mneharest) I3f Aj, IfH-tH( IH(I4)(Romi). The blond of 7H workers of n |*>ly(vitiyl chlo erences. W. C. Tobie ride) plant wax investigated. Fmimt were decrease of albumin Early silicosis. G. Worth, I,. Gnsthaus, K. Muysers, and F. and increase o{ jj- and -globulin (ructions in mtuiii proteins, di- oieholT (Krankcilliaiis (mfsclmrt, Moors, tier.). ISritr, Sili- a crease of the fi t<> ratio of serum li|><|)roteiiis, and dccri-asi- of kose-Forsek. No. 83, l-3( 19tf4). Physiol., patliol., and x*ruy the serum clnrflncstcrusc and psrudnctinlincuterusc activities, data were field, on Him coal millers aged Ut) HP years in comparison Blood catalase and serum pyruvic aeid were iiornmt. liT|0 with 23 healthy workers in the same age group not exposed to .Pathomorphology of brala elements of white rats with chlorodust. Early silicosis, beginning within i(> years, was detected prene poisoning. T. fl. Mnvscsyan, A. V*. Nlaulsiiknuyun, uml in 285 of the coal miners. Vital capacity of the lungs and forced __ C). Kh. didstyan. lev. A bad. Sunk . Inn. SSK. /Uni. .Xtinki 17 respiratory vol. were significantly lower in the miners, regardless of dust exinsure or the development of silicosis. Over 60% of the miners with early silicosis required a greater respiratory vol. (p). A1 -Kf 11H14X Russ). Results arc reported of hi.toi. invc-iigallons of the cerebral cortex of white rats <'\priMil (<> cliluropreiic (I) during tl months. The chronic effect of I in ilr>ses from ii.'si for a given uptake of 0 than did the 23 controls. The arterial mg./m.1 to 3.<Hi mg./m.J caused intense dystrophic changes in pOt in miners with pneumoconiosis was lower than the av., while the cerebrum. In general, all dystrophic changes in the cortex the olvcolo-artcrial gradient of 0 was higher, both at rest and b led to atrophy and wrinkling of sonic cells, swelling, tigrolysi-, during effort. These effects were related to time of exposure to and hypoclironmlnsis of other ceils and timdlv to the acute swell- coal dust, although not in correlation with the x-ray findings. Analysis of expiratory argon pressure curves showed that ven- tilatory troubles occurred in 5.6% of relatively young miners with ing and vacuolization and destruction of cytoplasm. The luxt changes are more sharply expressed in the elements beneath the cortex and medulla oblongata, fn the circulatory system, hypo- early silicosis from 10-13 years of dust exposure. Abnomudi- tonia of vessel wails and proliferation of the endothelium take ties of gaseous diffusion also occurred. However, there were no place. M. Charmendarian significant differences in the acid-base metabolism in early sili cosis as compared with that in the controls not exposed to dust. 16 references. `W.C. Tobie Mercury vapor in dental offices. W. Ketteri and K. Lam- Srecht (Univ. Munich, Ger.). Deui.Zahnaentl.Z. 19(12), 1104(1964). Hg contamination of dental offices may stem from open storage or from improper technique in prepn- of amalgams. Fingers contaminated with Hg are not completely free of Hg 20 rain, after thorough soap and water washing. Measures sug gested for minimizing the contamination of dental offices sure dis- Examinatioa of urine for porphyrin in some occupational poisonings. A. Petrova and >. Bakalov. Kkigtena (Snlia)4(4), 37-41(19G3)(Russ). Urinary rxatnn. for porphyrins was carried out in subjects with occupational diseases and in healthy individ uals. The results obtained are compared with those of liverfunction tests and urinary excretion of urobilin and urobilinogen. Increased urinary coproporphyrin excretion is s very early and sensitive indicator of liver damage. The investigation covers 19 forms of poisoning. 3 being combined. Coproporphyrinuri* bencountered in 13 forms of poisoning, viz., ligroine, benzene, Pb, CO, cussed. Henry M. Koehler -- Hg, Cu, S gases. TNT, MeCl. aniline, MejCO, KCN, and NaOH Hemocoagulatire modifications is chronic experimental poison- 4- liquid soap. A more thorough investigation was carried out log by tetryl. E. Daniele (Univ. Naples). Folia Med. (Naples) by performing urinalysis in subjects poisoned or subjected to 47(8), 767-76( I964)(ltal). The possibility was studied of detg. the noxious effect of gasoline. Coproporphyrinuria is a sensi- early or latent changes in coagulation and an increased tendency tive and more frequently encountered indicator in occupational to hemorrhage, esp. of the thrombophilic type, even when tests poisonings, even in the presence of extremely mild liver damage, prove neg. For subjects exposed to tetryl. The studies were d Urinary coproporphyrin excretion is very frequent in gasoline carried out on 15 rabbits; 8 were kept as controls and the others poisoning; it may even appear in apparently healthy individuals, were given 0.125 g. tetryl /kg. body wt. orally for 0 months, During the entire period the following tests were made: thromboelastograms, prothrombin time (Quick), prothrombinemia, who are in continuous contact with gasoline fumes. FmmAbstr. Bulear. Sci. Lit., Med. Pkys. Cull. 0(4). 34(1963). TCFR The maximum allowable cesceotration of dibutyl phtbalete. accelerinemia, eonvertinemia, and a direct blood platelet count_Mincho Spasovski. Khigiena (Sofia) 7(1), 38-44(I964KBulg}. (Palumbo and Dini). Blood was collected in a slliconated tube. The phys. and chem. qualities of di-Bu phthalate (1) are pointed After 6 months the following changes (in %) had occurred: out, the toxic parameters are established, and its acute and prothrombin time, from 96.3 to 68.1; prothrombinemia, from chronic effects on 299 different kinds of test animals are studied. 66.6 to 75.2: accelerinemia. from 95.9 to 96.7; eonvertinemia. The author det. 0.001 mg./l. of air as the max. allowable conen. from 95.8 to 72.3; plateleti/cu- mm., from 507,530 to 498,640. of tech. I, heated to over 140*. on the basis of examn. of condlBven though some of the factors did not change significantly, the a tioned reflexes, latent period, weight coeff. of liver and kidneys, general blood coagulation picture was definitely altered by chronic tetryl poisoning. The possibility of intravit8l hemolysis is considered. The most definite entity was the decrease in total body wt. motor activity, and fatigue. The toxicity of 1 depends on its routes of penetration into the organism. The vapors of I, heated to over 140*. are most toxic, causing like- coagulability. Felix Saunders BataWishing hygienic norms for some caprolactam interned!- ntei in the atmosphere. A. A. Dobrinskii (Central Inst. Ad- vanced Med. Training, Moscow). Gigttna i Santt. 29(12). 8-13 wise pulmonary edema. I is less toxic in peroral administration; it has no skin resorptive effect. It possesses a very strfeng cumulative effect when inhaled or applied by mouth. The cumulation COefT. is <t, explained by the development of high I sen- (IMHMKuss). The following concns. of cyclohcxanol (I) and sitivity. From A fair, Bulger. Sci. Lit., Med. Pkys. Cult. 7(2), cyclohexanone (H) in mg./cu. m.. resp., are reported: threshold Abstr. No. 469(1964). TCFR fordetection by odor, 0.24,0.21; by influence on dark adaptation Carbonic anhydrase in occupational lead poisoning. A. of eye, --, 0.11; detected by effect on elcctrocortical conditioned / Batolska. Sauchni Tr. Naucknoitsled. Inst. Okrana Trudo responses, 0.14, 0.11; recommended single-exposure max., 0.06, ' Prof. Zaboljavcnija 10, 55-9(!963HRuss). Examns. and lab. 0.04. Rats were exposed to aims, contg. 0.056 mg. I and 0.042 investigations were carried out on a series of 81 workers from a mg. II, resp./cu. m. for 81 days with no observed dianges in mus- Pb works. Besides the characteristic Pb-poisoning indexes, cular chronaxy, cholinesterase Ictivity, sulfhydryt blood concns., the changes in carbonic anhydrase activity were also detd. Simul- or ascorbic acid eonens. in brain and kidney, land II were detd. taneously with deterioration of the general phys. condition, as spectroscopically using the absorptions at 510 and 550 mx, resp. -- far as Pb intoxication is concerned, carbonic anhydrase wus John Howe Scott also depressed. Pb poisoning brings about a depression (already Hygienic evaluation of the synthetic flocculant polyamide, proved in vitro) of the enzytne two. From Abstr. Bulrar. Sn. Mark PAA. N. A. Rukhmauina (1. M. Sechenov 1st Med. Lit.. Mrd. Pkys. Cult. 0(4). 32(1963). TCFR Inst., Moscow). Gigitna i N'uxit Z9<12), 20-3< 1904)(Russ). Carbon dioxide transport in the blood of patients with silicosis The L.I>. values of polyacrylamide (I) for mice and rabbits are and silieotubereuloris. Khr. Tsolov. Nanchni Tr. \rau, ii- 12,950 and 11,250 mg./kg.. resp. These may be due to the 0 nmsttrd. Inst. Okrana Tritla Fra/. Zabrdjavaniju 10, 77-85( itUKIl monomer present. Doses of 1.6 mg./kg. caused eliaugps in the (Russ). The transport of COj in the blood is studitsl in 27n jm- eonditioned reflexes of rats in 9 months.The recommended max. tients afTectcd with silicosis (with varying degree of mnnife<.tu- conotl. of I in drinking water is2 mg./I.J<ilin Howe Scott tions of pathol.i and silicntulKTcnlosis. The pnrtiul CO. u>nsi>n Coaiosis baurda of a series of refractory materials. H. A. in alveolar air is relatively low, but it is higher than in urun.tl Katsnel soii, M. V. Lemystm-v. 1.. C*. Habnsiikinu, and L. N.__blrsid. The COi phys. dissolved in the blood is also <Utre;i'id. El'nichnykh l Med. Inst., Sverdlovsk), iltttrna i Santt. 29112). The total und phys. dissolved unit, of CO in the venous blond i- 30-8(llM>4)( Rush). ItiMs of various refrmiories were intro* within normal physiol, limits. The venoiis-siru-ri.il ililU-rrim-i- ducetl intratruvlu-idiy into ruts and Uh- eiTeets studied (hiring U insignifiemit. The progressive deterioration of the silicate inmonths histpi. and by detu. of hydroxyproUnv atid li;iid cuuriis, ducetl pnwcsR results in a comparative inervusr of the purtinl t'tv; The following groups were progressively less hurmful; quunzite tension as well a* <*f its phys. dissolved part in the arterial and and dinas; elianmite. day 112' . free Sdi.l, and foisierile; mag- ^ ,ai1l more in the venous blond, with corresimudlng divn-ase of Hie nosite; mid eliromoniagm-site. The reeomnjr-nded max. for these venous-arterial (lilTi'niire. A stale of h>p'K*upiiia is also oh groups are 1,2, 5, and Id mg./ni. m. air. resji. served in patients with silicnttihrreulnsis. The sepn. of C" lidni IImwi1 Si-ott from the hUs^i of pul u nis with eyiotosie iiv|H.seiuiii seeing 1........ Biochemical changes in workers occupied in vinyl chloride tin- most dillirnlt. wlierv-as in those with itncmie livpn\cini.i He syotbests and polymerization. Silvia C'.aiior. Mma Radu, N. least. The add-h.Lso luilancc in die him><i of patii-nts with n||i AP00008649 1231 Chemical Abstract* Vol. 66, 1967 Pas* US activity. G. V. Martinyan. Biol. Zk. Armenii 10(8), 36-41 (1066){Armcnian). In previous studies it was established that the dceotnpn. t>( proteins is accelerated by poisoning with chloro* prene. litis process can be interrupted and the protein Con tained in cells rapidly restored with hyposulfite soln. by an un known mechanism. Studies made to elucidate the influence of ehloroprcnc and hyposulfite on eathepnn enzymes were incon clusive, and it is suggested that the most important factor in this modification is related to the transaminases. Bxpts. were con ducted using normal rats (control) and rats poisoned with chloro* prene (8 mg./l., 2 hrs. daily, 2-3 months). The activities of glutsmic-oxalacetic transaminase and glutamic-pyruvic trans aminase were measured by usiog the ReUntaii and Frankel method (CA SI, 18080A) and are expressed in Vroblovski units. The activities of the enzymes decreased 10-16% in blood, liver, and kidneys and 41--51% in the spleen of poisoned animals. In In vitro expts. the decrease in entyme activity in normal tissues was 4.7-8,4% and in poisoned animals2.3-7,1 %; inthe spleen the decrease was 0.4-0.5%. Hyposulfite also lowers the enzymic ac tivity; however, the combination, hyposulfite-chloroprene re stores it to the initial level and in some cases even a higher level is reached. Chloroprene does not affect the activity of pyridoxal Ehosphate. These results lead to the assumption that th* inibitory action of chloroprene can be explained by its reaction with the protein part of the enzyme. L. B. Bedighian 1231c Changes In the estetined fatty adds and cholesterol in serum and Uver of rats after application of orotic acid and hepato- toxic substances. F. Musil and T, Sueva (Skoda-Werke-Krank- enhauses, Plzen. Czech). FetU Seijett Anslrithm. 68(9), 748-60 (1968)(Ger). Changes in the serum and hepatic levels of choles terol and esterified fatty acids following oral administration of CCU (0.5 ml./kg.) for theintraperitoncaladministration of ethio- nine (250 mg./kg.) to rats were in some cases normalized follow ing oral administration of orotic acid (100-250 mg./kg.) given as either s preventive or as a therapeutic agent. CMJG 1232k Acut* (mouse and rat) and thort-tsrra (rat) toxicity studies mi dibutyKdlethylane glycol blsphthalate). O. B. Hall, Patricia Austin, and F. A. Fairwcather (British Ind. Biol. Res. Assoc.. Carshalton, Engl.). Food Cosrrnt. Toxicol. 4(4), 883-8(1966)(Bng). Acute and short-term feeding studies have been carried out on a sample contg. 80% dibutyl (diethylene gly col bisphthalate) (DDGB). The intraperitoneal L.D.h In both mtee and rats was approx. 11.2 g./kg. This dose when given orally was tolerated by both species without ill effect. The toxicity by either route in mice was enhanced when DDGB was administered In arachis oil (50% vol./vol.). In a 90-day study in rats given dietary levels of 0.0 (control), 0.25, 1.0, and 2.5% DDGB, significant growth retardation occurred in both sexes at all levels apart from male* on 0.26%. This effect was attribut able, at least in part, to reduced food intake, although this effect was less marked in females. There were no hematological changes or effects on kidney function. At the 1.0 and 2.5% diet ary levels, the relative liver and heart wts. in males and the rela tive brain wts. in both sexes were signifieantiy increased. No pathol. changes were found that could be attributed to DDGB. In view of the effect on growth at the lowest dietary level of 0.25%, a no-effect level in the diet of rats for 60 days could not be established for the particular batch of DDGB tested. RCTT I233t Reaction of bydroxoeobalamia with thiols. Norman Adler, Thomas Medwick, and T. J. Poznanskl (Merck Chem. Dhr., Merck 8c Co., Inc.,-Rahway, N.J.). /. Am. Cham. Soc. 88(21), 5018-20(1966)(Eng). Hydroxoeobalarain reacts with thiol compds., as exemplified by glutathione, to form relatively weak 1:1 inner coordination complexes. Previously reported in consistencies in the generality of this reaction are explained in terms of the simultaneous role of thiol compels, as complexing and reducing agents. RCJC 1234a Heerotoxie effects induced by alkylating agents. M- G. Donelll, R. Rosso, and S. Garattini. J. Pborm. Pharma col. 18(H), 7GO-2(10C6KEnfi). In decreasing order of effective ness, di^tryptophan mustard, o-sarcoiysinc, glycine mustard, l- sarcolysinc, pa-sarcolysine, and alanine mustard produced a typical pattern of neurotoxicity when administered intraeore* brally to rats. The typical pattern of neurotoxicity included a iateney time of about StV-tUI mm., followed by signs of central stimulation, incoordinate movements, aixl stereotype behavior, otid ending with clonic convulsions ami itcciisinuul tonic exten sions. Mannitol mustard. tetruuiiue, cyclophosphamide, and chluranibucil were not nvnrotoxie when udin'mislcrvd in doses of 100 y/rut. lntrapcritonral administration of Mu phruytniti (KXJ mg./kg.) was ineffective in preventing tin- lu-urntoxie effects in duced by the alkylating agents, w herons Na pheuohurbitouc showed a dear protective effect. Cysteine and thiourea (1 g./ kg., intrapcrittmcally) were also ineffective in preventing tile neurotoxic effects. CMJN' 12ISh Effect of chronic nortriptyline pretreatment on the acute toxicity of various medicinal agents in rats. 1). lb Meyers, D. O. Kanyuck, and k. C. Anderson (Idi Lilly Hr Co., Greenfield, Indiana). J. Pkarvi. Nti. 55(11), m7-lK(llifiKliit)0. A method using rats that can be of value In predicting the effects of chronic treatment with psychotherapeutic* on the acute toxic, ity of other medicinal agents is described. Results obtained by this method showed that nortriptyline, like amitriptyline, en hanced the toxicity of most cerebral depressants in an additive manner. Both of the aatideprettive compds. markedly potenti ated the toxicity or physostigmine and to a lesser degree pilocar pine. Ephedriuc toxicity was significantly antagonised by thymolcptic pretreatment. The test failed to verify reports that the tricyclic antidepressives dangerously potentiate the toxicity of ale. or monoamine oxidase inhibitors. Results indicated that a variety of medicinal agents can be used in the nortriptyline treated patient without any problem of siguifieant drug inter action. RCYG 1236r Chlorinated anilines and their sanitary-toxicological characteristics. G. D. Khamucv. Khim. Faklory Vucshn. Sreiy i ikk Gipen. Znachenie, Sb., kfotcew 1665, 108-10(Russ). Monochloreaniline is used in the production of insecticides and dyes. _ m-Monoehloroaniline (I) and p-monoehloroaniiine (H) dis solve in HiO (6 g./l.), in which they cause an unpleasant taste and odor. The odor and taste perception thresholds were 2.6 and 7.2 for I and 1.5 and 3-5 mg./l. for II, resp. In a concn. of 150 mg./l., I and II lowered the B.O.D. of water. Threshold concns. are 1.5 mg./l, for I and 0.75 mg./l. for II. The L.D.* values (stomach administration) for I and II, resp., were 1104 and 401 for white mice, 1104 and 368 for white rats, and 750 and 350 mg./kg. for guinea pig*. A 3-nxmth sub-acute expt., conducted on white rats with stomach administration of 0.1 L.D.is levels, caused the formation of mcthemoglobin and re duced the hemoglobin level and the erythrocyte count. From Ref. Zk., Khim. 1666(8), Pt. II, Abstr. No. 81414. MVRK I237y Toxicity of thiotcetamide. Z. Hniban, W. Grad man, A. Slescrs, and M. Lubran (Univ. of Chicago). Lab. [mttt. 15 (11), 1746-50(1966)(Eng). Thioseetamide (I), fed to rats in a diet contg.' 50 mg./kg. body wt./day for 5 days, produced high serum lactic dehydrogenase levels end centroiubular necrosis of the liven within 24 hrs.; the entyme levels decreased and the necrotic areas were resorbed within 3 days of feeding the diet. Severe degenerative changes in the kidneys, hematuria, and in creased serum urea N and serum creatinine levels occurred within 48 hrs. of treatment. The lethal effects of the above level of I were correlated with the renal damage. Excess dietary casein hydrolysate, methionine, or guanosine reduced the mortality and renal toxldty of I, although these compds. did not prevent the nuclear and nucleolar enlargement of hepatic cells and of the cells of the proximal convoluted tubules of the kidney occurring in treated rats. 67 references. BFJN 1238f Poisoning by grass. C. H. Gallagher, J. H. Koch, and H. Hoffmann (Univ. Sydney). New Scvntiit 31(510), 412- 14(1666)(Bng). Acute and chronic neuromuscular disorders occur in sheep feeding on the pasture grass Pkalarit tuberosa. This grass contained 0.3% (dry wt.) tryptamitte alkaloids. Tissues from sheep undergoing acute poisoning showed no histol. alterations, whereas the brain and kidneys of ebronieally poisoned animals showed deposits of green pigment. The pigment was noted in the mitochondria cells of the mid-brain, brain stem, and spinal cord. It is suggested that these changes are related to the oxidative deamination of Af,Af-dinxethyltryptamine and 5-mc- thoxy-N.N-diraethyltryptamine. The protective action o! Co against the chronic form of the disease, reported by others, may be related to the prevention of degenerative changes in nerve tissue or to the acceleration of alkaloid metabolism. W. L. Downs !236p Viayi chloride; industrial toxicologic aspects. I. Grigorescu and Gh. TJna. Rev. Chim. (Bucharest) 17(8), 499- 501 (1966 )(Rom). A hypothesis was put forward, daimiDf that CH,:CHCI (I) could react with HiO at the level of the hepatic cell, yielding ethylene monochlorohydrin, which could be oxi dized to chloral and ohloroacotic acid (II). U couid be elimi nated as such in the urine or be metabolized further through amination to glycocol. An anal, method was developed for detec tion of II la urine, assuming that the only other source of II could be massive ingestion of wine yeast or inhalation of trichloro ethylene. An alkiuot (20(1 cc.) of urine, acitlifu.il with 50* r HiSOt (2 cc.) is extd. with Et*0 (200 cc.), shaking vigorously, and settling for 24 hrs. The ext. is dried (unhyd. N'a,S0ii. vvapd. to dryness under reduced pressure, and tin.- residue is di->- solved in 50'", uq, ulc. soln. (0.5 cc.). One drop <( tlie (25-30 al-) is placed on Whnlnnui No. 1 paper stri|w (25 N 3 eui,), dried, uiwl chrmtmlouruplu.il by ascending itn-tlu,,!. using as clutnnt the mixt. NH<OII-KtOH-II,0 (1:20:4) (nr a period of 6 hrs. The strips, dried in air, urv kept 24 hrs. in ;i coned. N'Hj mm., heated for 5 min. at 80*. sprayed with 0.11 i ninhydriti in McOH, and dried. The II is iudicuttil by reddish- violet spots. For distinct development, the strips are- spr.ivid finally with 10% Cu(N()a)i (carmine red spots). The Hi ut 21 ' wus established u-ith u soln. of 20-30 pg. 11/cc., as O.Xd, 'I lf limit of sensitivity is 0.1 ait* II, ami is improved by the me >f C'n (NGj)s. The extn. of II from urine was demonstrated t,y trail ing a soln. of 26 pg. II/cc. in urine us above, cutting tin- nine- AP00008650 fad U 14--Toxfeelogy Vol. 66, 1967 1249 ponding spots, eluting the II with EtOH, and dct*. eolorimetri- cslly. The cxtn, yield was '"80%. Preliminary tests showed no U in elin- healthy personnel, while among tile staff of I-proe- casing plants, 80% of the sampled persons gave a pot. reaction to the method. Extensive tests showed tliat most of the pos. reactions were obtained with staff employed Id-5 yean in the plants, in these eases the protcinograms were modified, the aglobulbt fraction being higher and the v-globulin fraction being lower than in the persons with ncg. reactions to the pres* enct of II in the urine. It was concluded that the capacity of the organism to metabolize XI decreased after 2 years. M. Ben Elicscr 1240g Effect of hex*chloro-p-xylane ingestion on the oxidative phosphorylation of rat liver mitochondria. Hsia-Ying Chang and Chen-To Yuan (Sino-Sovict Friendship Uosp., Peking, China). Stent IIV Hua Hsuek Yu Stunt Wu Wu Li Hsuek Poe 6(3), 273-5(1906)(Ch). Oral administration of hcxaehloro-pxylene (1,0.6 g./kg./day) to rats uncoupled the oxidative phos phorylation of the liver mitochondria, in extent proportional to the duration of I treatment. The decrease in the production of ATP with insufficient energy supply of tissue may be the basis of the toxic reactions of I such as generalized weakness, fatigue, ditsiaess, headache. BHJJ 1241a Adrenaline-inactivation capacity of the normal tad of the CClt-poisonsd liver perfused Is vitro. Emma Costiner, L. Vaislcr, and Viorica Chfvu (Acad. Rep. Soc., Romania, Bu charest). Sludii CenetariEndocrinol. 17(4), 355-7(1966){Rora). Normal rat livers, and liven originating from CCU-trcated rats were perfused with adrenaUue in vitro. The latter showed a lower rate of adrenaline degradation as compared with the former. BTJQ 1242x Pyruvie add contest is acute barbiturate poisoning. B. F. Murashov (S. M. Kirov Mil. Med. Acad., Leningrad). Ter. Arkh. 38(10), N)3-4(l066>(Russ). In deep coma due to barbiturate intoxication, 1.2 rag. % pyruvic acid (I) was found in the blood. Its eonen. decreased with recovery to 0.77 mg. %. When the I eonen, reached 1.8-2 rag. %, complications occurred sad the respiration was disturbed. The X eonen. was directly proportional to the severity of intoxication, as observed in a total of 32 poisoned humans. Miloslav Kalab 1243# Inhibition of biosynthesis of protoin and nudeie acids by phenolic compounds in vivo. G. V. Kukushkina, L. B, Gor bacheva, and N. M. Emanuel (Inst. Chem. Phys., Moscow). Vofir. Med. Kkim. iJ(5). 462-a<1966)(Russ). Mice with the Ehrlich ascites carcinoma or solid hepatoma }UCIi were injected with a mixt. of uniformly uC-)abelcd L-amino acids (1) (10 *ic./ mouse), Na formate-14C (XI) (5 ^c.) or adenine-6-MC (III) (17 je.). After 30 min., one group was injected intraperitonealiy with Pr gallatc (IV) is 0.9% N'aCl and another, with 4-methyl- 2,6-di-tert-butylphcnoi (V) tn 3% aq. Tween-80. Controls were injected with the reap, solvents. Ascitic fluid samples were taken from mice with Ehrlich tumors at time intervals from 0 to 180 nun. and the ceils washed with a 5:4:1 raixt. of 0.9% NaCI, 0.3M KaiHPO,, and 0M KH|PO. Mice with solid tumor*wre sacrificed after 120 min. and the tumors, liver, kidneys, and spleen were removed and homogenized. When 1 was used, the cells were treated with cold 5% CUCCOOH and the pptd. pro teins isolated and examd. When 11 and m were used, 5% Cl*- CCOOH washings were extd. with Et*0, the aq. layer evapd., and the residue (add-sol. fraction) examd. for radioactivity. The add-insol. fraction was washed with cold 98% EtOH and heated for 10 min. at 60* with EtOH-EtgO (2:1) to remove lipids. A sample was examd. for radioactivity and the rest extd. with 10% KaCl (pH 7.4--7.6) for 30 min. at 96*. Nucleic acids were pptd. by adding 3 vols. of cold 96% EtOH and washed with 70% EtOH. In mice with ascites tumors, injection of 75 ing./ kg. of V decreased I incorporation into protein by 30% as com pared with the controls; 2U0 ing./kg. of V inhibited I incorpora tion almost completely. Injection of 100 mg. of V/kg. de creased XH incorporation into nuckde adds by 20%; 200 mg. of V/kg. inhibited III incorporation almost completely. Injec tion of 80 mg. of IV/kg. or of 120 ing. of V/kg. alter 160 min. de creased II incorporation into acid-insol. fraction by 43-53%, into nucleic add by 54-01%, Into odd-sol. fraction by 50-77%, und into proteins, by UO--14%. In mice with solid hepatoma, in jection of 150 mg. of IV/kg. after 160 min. decreased I incorpora tion into tumor proteins by 20'.,; 200 mg, of IV/kg. decreased I incor|K>rati(Hi by 113%. injection of 150- 200 mg./kg. of IV had no effect on proteiu biosynthesis hi kidneys ami the liver of normal mice mid hi kklneys of tumor-henring mice which showed some activation ol I incorporation in the liver- Injection of 200 mg. of IV/kg. decreased 1 incorporation into spleen proteins of normal and tumor-bearing mice by 30%; 150 mg./kg. had no effect on protein biosynthesis in the spleen. Felix Burck 1244a Experimental Intoxication by ammonium salts. Pro tection by some amino acids and their mechanism of action. F. Viiuinn utul K. Snlvuton- (Univ. Naples). Hioelimi. ,1 f>fil. 13 57- K5(11MKI)(Uul). The bitiehein. coiivitmou of NTlj into nr a is discuwiul relative to tin- protection afforded in acute ami chronic Nils intoxication by snuie cihu|m|s. uSM>cd. with the con version process. Exptl., acute NH, poisoning was produced in rats by administering L.D.u N'HOAc, preceded by varying doses of protective amino acids and their mixts. Based on mortality and convulsions, arginine (I) provide* the most effec tive protection at as low a dose at 0.5 reillimole/kf, A mixt. (A) of L-omithine and L-aspartic acid at a dose of 1.0 millimole/ kg. protects as effectively as I. A comparison of blood NH* and urea levels using I and A shows X to be more effective a pro tector than A. In rats intoxicated with a L.D.u of NH0Ac and Eirotccted by A, a-mcthylaspartic add injected intraperitonealiy a s dose of 3.4 millimolcs/kg. produces approx. 54% mortality and 90% convulsions. Utilizing this same procedure but sub stituting I for A, the mortality and convulsions percentages de crease. Further, a doss of 0.5 millimole 1/kg. produces 8-8% mortality and 20% convulsions, while a dose of 1.0 millimole I/kg. produces 2L% mortality and 38% convulsions. 67 refer ences. DomenlcA. Vavala 1245v Erythrocytic ALA dehydratasi sad lead, D. Bonsig- nore, P. Calissano, and C. Cartasegna (Univ. Genoa). Fan- Mtuenw Med. (Engl. Ed.) 8(7-8), 282-4(1966)(Eng). Persons having Pb poisoning showed an increased urinary excretion of aminolevulinic acid (ALA) (I), which was assoed. with a sig nificantly decreased activity of I dehydratase in the erythrocytes. The assay of I dehydratase may be of significance in assessing the gravity of pb poisoning, since in exptl. animals the level of I dehydratase was related to the extent of the poisoning. B. S. S. R. Rao 1246c Relations between the porphyrin metabolism and the nicotinic acid metabollam in saturnine poisoning. L. Pecora, A. Silvestroni, and A. Brancacclo (Univ. Naples). Panminerva Med. (Engl. Ed.) 6(7-8), 284-8(1966)(Bng). Poisoning of rab bits with Pb(OAc)* decreased nicotinic acid (I) by 75% in the blood and urine and markedly increased erythrocyte-free proto porphyrin (II) (13-247 y %) and urinary coproporphyria (III) (14-341 -y/24 hrs.), aminolevulinic acid (IV) (0,2-21 mg./l.), porphobilinogen (V) (trace-6.6 mg./l.), and xanthurenic acid (VI) (1.0-18.3 mg./24 hrs.). I administration either concurrently with or 15 days after Pb poisoning increased nicotiaemia and nicotlnuria and markedly inhibited (by 50-80%) the effects of Pb poisoning on Q, III, IV, V, and VI. The effects of Pb poison ing are probably mediated through inhibition of pyridine coen- syme synthesis directly or indirectly through vitamin B* inhibi tion. HFJN 1247k Porphyrin* in tetraethyl lead poisoning. M. Crepet and P. Chiesura (Univ. Padua, Italy). Panminerva Med. (Engl. Ed.) 8(7-8), 295-301(1966)(Eng). The levels of aminolevulinic acid, Pb, and porphyrins were detd. in blood and urine samples of persons having Pb poisoning due to inorg. Pb salts or Pb tetraethyl and also in expel, animals with exptl. Pb poisoning. Pb poisoning due to Pb tetraethyl caused'a significant increase in the levels of free protoporphyria in erythrocytes, while Pb poi soning due to inorg. Pb salts caused an increased urinary excre tion of aminolevulinic acid and coproporphyria. B. S. S. R. Rao . I248t Aflatoxin B( injury In rat and monkey liver. Donald J. Svoboda, Harold Grady, and John Higginson (Univ. of Kan sas, Med. Center, Kansas City). Amer. J. Pathol. 49(6). 102351(1966)(Bng). Oral administration of pure aflatoxin Bt (0.45- 3.78 mg./kg.) or intraperitoncal injection of a 0.167-mg./kg. dote into rats produced periportal parenchymal cell necrosis and slight bile duct proliferation, together with sepn. of the granular and fibrillar components of the nucleolus with the formation of nucleolar caps. The alterations in fine structure were also found ia monkeys after oral administration of 0.46 mg. purified afiatoxin Bi/kg, or intraperitoneal injection of a 2.5-mg./kg. dose. The livers of rats receiving an oral 0.45-mg./kg. dose 24-72 hrs. before sacrifice showed decreased levels of RNA and protein in both the nucleus and cytoplasm. The RNA-to-DNA ratio in a total liver homogenate decreased by 40%, and that in a nuclear fraction by 8%; the protcin-to-DNA ratio in the total homoge nate decreased by 24%. P:0 ratios showed decreasing values with increasing functional damage to the hepatic mitochondria after administration of aflatoxin Bi. Both O consumption and phosphorylation were Inhibited, although the essentially normal O consumption 72 hrs. after administration of the toxin iuay have indicated recovery of respiration without the concomitant return to normal phospiiorylation levels. The acute hepatic lesions shown by light microscopy of liver from treated numkoys re sembled the changes in humau liver during acute viral hcfiatitU. Administration of pure aflatoxin Bi (i ppm. of a synthetic diet fed ad libitum to rats for 33 weeks) did uut prtxlucc the nuclear, nucleolar, and cytoplasmic alterations found after the acute administration, although wed-differentiated hepatocellular carci nomas appeured in rats whose livers otherwise showed little ab normality. 55 references. Bl'JN 1249a Distribution and excretion of cyeUmat*-uC sodium in saim&ts. Jonathan 1*. Miller, L. B. Michael Crawford, Robert C. Sender*, and Kurt V. Cardinal (l)vpt. of I'luirnmcoL, Abbott l.ulis.. North CUirugo). liiuflwui. lUoftkys. Res. Comm. 25(2), 153-7(ilXKi)(Kug). Nu cyclutuati.-l,C distribution, excretion, AP00008651 3U0U '3O0$ toxicology lai. w] nm streptomycin an^orhlnatr. tHhydro*'r^rfomvf In panthoteMte, peeo- mretft. Ilorlmyrln and kanamyeln are presented. Complex contpoimd* 'thydreelreptoinyeln with atcorMc, ^Mhotantc or p-arnlrasalycllle i re ten* tnxle than streptomycin sulfate or dinydroetreptocnycin. .AJ1 above dlhydrortreptomyeln derlrotive* may be used In mei with side rffeetn ol streptomycin aulfate. Panomycln ta preferable atnee it combine* 3 mala antituberculous preparations (dihydrostreptomycin and PAS). Fierimye In and kansmycln arc more toxic . than itreptomydn satiate or dlhydreslroptomycln tad their derivative*. These antibiotics should be limited by patients with progressive procesaea and streptomycin resistance. Ftorimyeln and hxaamyelx are ototesde and should be need only la stationary conditions with constant examtnstlon cf hearing.*-Authors* 1 30951. TATLOR, T. K. M. L. MORRIS, and JEAN HRKLEY. (Agr. Res. Coune. Poultry Res. Cent., Edinburgh, fieotn UK.) Effect of dietsrv excesses of vitamins A and D on some constituents ot the blood of chicks. WIT 3 ffllTft 71PT3I. ` IWI.-flrW of chick* were gtven diets containing 4 levels of vitamins A and D, l, to, too and tOOQ times the basal level, in all 1$ COmbinatteas, with the object at Investigating a possible antagonism between the 3 vitamins. Only dtsts containing 1000 time* the basal level (appreau 1700 ttmee the dietary requirements) of 1 or both vitamins depreeaed growth and induced ehangs* in the blood. The packed cell volume was substantially reduced trom 4 wk of age In the chicks gtvan tht highest level of vltimis A. This was probably due to an effect at the vitamin on the fragility of Uie red cells and thus on their life span. Chicks gives the toxic level cl vitamin D ahowad an Increase In plasma Ca aad a decrease la plasma inorganic p. The highest level of vitamin A depreeaed plasma Ca without Influencing plasma tnorgaalc P. Increasing amounts of vitamin A given In combination with the highest level of vitamin D caused a progressive increase in the plasma Inorganic p, the highest level ol dietary vitamin A significantly increased the activity tn the plasma of 3 lysosomal enaymea; add phosphates*, d'gktturoddase and arylsulghataee. Excess vitamin D given ta conjunction with the basal level of vitamin A slgaUicartly dipresstd the plasma setd phosphatase and the activity of this exiyme increased with Uteressing amounts of vuamtn A. Excess vitamin D had no influence on the ether hydrolases studied. A marked antagonism * between the effects of exceailve amount* of the 2 vitamin* occurred only tn rtspeet of their sctloae on the plasms levels of Ca, tnorgaalc P and acid phosphatase, all of which are involved m bone metabolism. --Authors, 30952. TUMARWN, R. I. (N. F. Gnmaleya Inat. Epidemiol. Micro biol., Acad. Med. Get. USSR, Moscow, USSR.) k voprosu o mckhaalsme teksieheskogo delstvlya lntlWotlkor tctratsikUnovol gruppy na peehen'. fMechssiam of Uie toxic effect of the tetracvcllne crouo of antibiotic* m~fke llvlt.l ANnWRET'13{7T: tVTtST. 1968. lEagi. worn.I --Clilortetraeyellne in Mpi doece In rats increased histidine deaminase and urokaniatse activity. Oxytstrncyelin* had a alight effect on histldlnedetmUUtae activity and suppressed urokaxlaase activity. Tetra cycline effect was similar to that of ojcytetracycUne. Chlortctraoycllns has a higher toxicity .--Author. 30953. TYVL'MANKOV, V. N,, and B. K. LUOOVSKH. Sosudictyc oelochenenlya pri leeheail psikhieheskl bol'nykh amlnaslnom, rvaseular cemnlleatlons durtnx amlnaslne Fchlorbromaxinel treatment mental mUenis. I ZHf NEUROPATOL WUtHDlT'nrS rgQRBAKflVTl Tll-llS."" i98l. [Engl, sum.)--The main syndrome* were eerebral hemorrhages and thrombotic vascular affections of vitally important organs. Amtnaxln* probably increases coagulability. A thorough control (Including laboratorlal and tests) of old patients aad ibUcms with hypertensive disease must be made In the preset* of amtaaxlse treatment.--From auth. sum. T 30954. WEISS. HARVEY 3. (Ml. Sinai Hosp.. New York, N. Y., USA.), LOUIS M. ALEDORT. and 9MAUL KOCKWA. The effect of ealtevlates on the hemostatlo orooerUeo of platelets in man. J N INVEST 41(3); rU*. iSW.-WesWdn ol 1.5 g of aspirin, but not of sodium salicylate, produced a significant promo tion of the bleeding time In 8 normal male aubjeet* when compared with the effects of a placebo. Similar dUfereacea In the effect of the 2 drugs on platelets was also observed. Aspirin ingestion resulted in Impaired platelet aggregation by connective tiaeue and was associated with a decreased release of platelet ADR; sodium salicylate had no effect on these values. In vitro, incubation of platlet-rteh plasma wnh an optimum aspirin concentration of 0.045 mg/ml inhibited both ihe adhesion f platelets to connective ttesue and the release of ADP as well as the 2ndsry wave of platelet aggregation produced with ADP or epinephrine. Sodium salicylate had no effect on theoe reactions, which were also normal In patients with von Wlllebrand's dlaesae, ? inhibitory effect produced by ingesting a single 1.4 g deae of ptrln was detectable for 4-7 days at whleh time salicylate was no ..oncer detectable la the blood, which suggested an irreversible effect " on the platelet. Aspirin also inhibited the release df platelet ATP, but had no effect on the platelet surface charge, available platelet ATP or ADP, or Uw dsatruetioo of ADP by plasma ADPast. Thtto studies support Ihe hypothesis that Ingestion of aspirin, lfl contrast to sodium salicylate, prolongs the bleeding time by inhibiting the releaae of platelet ADP, perhape reflecting the findings In other cell yterns that aspirin alters membrane permeability.--Author*. 30953, ANONYMOUS. The kidney and oral calcium therapy. ANN INTERN MED S(S): 1821-IM!. IBIT-Though Ca65Jta valuable aa an nstaeid, severe and possibly irreversible Injury te the kidney may occur from Its prolonged use In conjunction with a high intlklnUke. Evidence of renal Iunction disturbance may be shown by a rtne in serum creatinine and renal N. During trentmeat with CaCOS a patient's serum Ca level should be watched for early eigne of Cs neuropathy within the 1st 7 days of treatment. INDUSTRIAL TOXICOLOGY (See also "Pathology" undtr Respiratory System: "Environmental Health - 'Occupational Health, * and 'Radialloa Health' ~ under Public Health) 30958, GANDEYIA, BRYAN. (Dlv. Thorac. Med.. Untv. N. g. W.. Sydney, N. 8. W.. Aust.) Pulmonarv function In asbestos workers: A three-year follow-up etuorT aMek *v Rtip pis 96{3): T8P 427. Ulus. [Span, and Fr. sum.]--Ventilatory capacity aid ventilatory response te a standard exercise test war* measured ta a series df 41 mats workers In a factory making asbestos products, aad was repented about 3 1/3 yr later. Twelve mes left the Industry during the intermix. They had a etgnUleantly tower vital capacity after adjustment for age, and a significantly higher ventilatory requirement for exercise than those who remained in the industry. This pattern of lonetloaxl abnormality contrasts with that found la subjects admitting to psrtlstent eough and iputvim, tn whom a significant reduction was demonstrated in age-adjusted forced expiratory volume at l see. In a control series, observed and predicted changes In pulmonary function over Uw observation period showed reasonable agreement. The asbestos workers showed a greater decrease In forced expiratory volume at 1 sec. than predictt< aad possibly greater changes Is vital capacity end ventilatory require ment tor osorels*. The trend toward tuaetlcnal abnormality Is con sistent with, although not Indicative of, the development of asbeetaela, Serial pulmonary (unetton teste tee likely te be more Informative than serial roentgenograms.--From tuth. sum. - 309B7. HARRIS, D. KENWJN, xml W. G. F. ADAMS. Acro-osteofrHs occurring In men engagedln the polymerization of vinyl chloride. ' BfUt'MED 3 5987, 712-714. LiUl. HSt.--TWO CSSS* Cfl aefooateolysis occurring tn men engaged In the polymerisation of vinyl chloride arc described. The bones affected were the terminal, phalange* of the fingers and the eaeroUtae foUrts, but in 1 ese the potelU and In Uie other the phalange* of the feet were Involved. The condition wax-accompanied by Raynaud's phenomena and akin tesloaa. TMa eondttlon ta probably a aalf-limiting dtecae*.--Authors. 30958. SAMEDOY, L O. O nespetslftchaoldkh proyavleniyakh delatvtya uglevodorodov v malykh kootsentratslyakh. (Nonspecific manifestations of low concentration* of hydrocarbon*.1 AZ.ERB MBP'ZH d. 5M1. 1887. From; TgragWDWP FARMAKDL KHMIOTER 8REDSTVA TOKSDCOL, 1988, No. 4.84.915. (Translattcnj -Workers war* teated at a pctroleum'proccaalng plant where the concentration of hydrocarbon vapors did not exeeed the maximum ailowabie (0.07-9.48 mg/1. Rabbits and guinea pigs were treated under various static regimes with the vapors of gasoline B-70 st 0.05-1.t ml/1, 4 hr/day, 6 timee/week for 4 mo. The worker* end the animals showed a rise In the concentration of btood icatylehollM and eplnepbrlnn-llke substances. Animal* treated constantly and evenly with Increasing hydrocarbon concentration* shewed these tendencies, but they were leas marked. Cholinesterase activity in the workers did not differ from that of controls, but it was reduced tn treated animals, especially when the treatment was with fluctuating concentrations of hydrocarbons. PseudochoUneaternae activity did not change to etther persons or animals. Dally urinary excretion of 17-ketosterotds by treated rabbit*, was of a phasic character: the elevated activity of the adrenoeorucal function was soon replaced by reduced activity. Only under the setioa of sharply fluctuating hydrocarbon concentrations was there a prolonged rise in 17-ketosterold excretion. Maximum depression in die concentration of vitamins C and Bj In the blood, urine, and organs was noted tn animals treated with steadily and evenly increasing and also sharply fluctuating hydrocarbon concentration*. Changes la the electrolyte metabolism with low concentration* was insignificant. The principal changes with low hydrocarbon concentration* were in the functional condition of th* autonomic and endocrine system* (chaitfes in the metabolism of mediating substances and vitamins and tn adreno cortical function). Theee change* are probably nonspecific and of a compensatory-adaptation*! nature. Over a certain period they may lead to persistent and even organic change*. 30954, BPAK, IYAR. (Dtp. Surg., LanaUaarettat, Husletooln, Bw*d.) Fluff Injury caused Nr nalnt way aun ACTA CHIR AP00008652 -43B40-1J9M TCBDCObOOT (Vol. 31) 4311 Dias. t968|r#ccL 1969J. (hi jap. with Eng), sura.)--For the pollutant* produced Up exhaust gas from motor vehicles ana blaming gea from ' imintto, carbon mondtlds, nitrogen oatdeo and drat were detarnitaod Ida the NUionzal* Tunnel under eenairwctloa lor the Tokye-Nagoya oxpreeswty. The pollutants obtained near the bleating pint* allowed 'high concentrnllona of carbon monoxide, nitrogen caMee and tha diet, with levwls of 350-1,200 ppm, 10-3* ppm, and 5.3-60.5 ntg/m*, respectively. Thaos reaulta wars die to defective rsidUatton, 43940. MQtIONA, K. 8,, and V. U BRMOLJNSKXL O stuchayakh otmvlentj* rtut'yw srtdl medUBinlklkh rnbotnHcav. {Caere nf mercury polsonlnc among medical workers.1 SB NAUCK TR KUIBYSHEV HAUCH-ISSUED fkST EPIDEMl6i GtO 5. 83-33. 1938. Translated from HEP ZH OTD VYP PARMAKOL XHIMIOTER SREDSTVA TOKSIKOL, 1981, No. 5.34.728. --Observations were made of 4 eases of Hg poisoning to medical personnel as a result of tofraction of 0\s rules tor working with apparatus containing Hg. Tht studies, whldt were carried out la several medical Institutions, revealed the prwaaKi of Kg In wsshlags from equipment, wills, Doors and la tha air of the location. In a number ol cases tha concentration of Kg slplficauUy exceeded the maxlnmm allowable camsidmttca. Working with apparels* centstolng Hg can be a considerable danger when the rulee tor safety tsehnlqvad are not limned. 43341. IVANOVA. L 8., and M. M. MEL'NIKOVA. (Coat InSt. Postgrad. Mad., Moscow, USSR.) K vcprosu o sestoflnU sdoror'ya rabochlkh r prolsvodatva attrobekl. fProblems regarding the health milHtm* nf worker* to nttraesn-ekeeDhoros-ootaettem ierttlteer hciorles.l OM Tft Pho^ tAbOC 12(11):'49-ao. hm.-Observa tions conteieted oa 181 workers, disclosed the presemes of different degress of bronchitis, which ware observed to marked and ftutctlooal disorders of the nervous system to the term of vegetative dysfunction. The effect ol s number of substances, having a stimulating ofleet (such hs chlorine and eulNrou* compounds) had a significant roU to the development of bronchitis. Changes occurring to the asm system ire eosasetsd with lbs sfteet of a complex of thtslal subataaoee. --l o. a 43942. BUSHUEVA, T. L, E. A. ZABOZLAEVA, and A. Y. YAKtfBOV. K voproeu s datsbrll yndokhtmlkato? na akUesoftt' eyvoretochnykh fermsntor I belkoruyu karttou krovL fThc action of chemical notions an the serum enzyme activity and the blood protein picture. I TR \A6ZfTM> twV " w. 139-141. 1^06. Translated Iron HEP 7X n> VYP PARMAKOL XHIMIOTER SREDSTVA TOKSIKOL, lMt, No. 8.54.939.--When cottea was being prayed with organophaepbaraa bwectietdst, tha concentration of them to the air ol the working sons was 0.00002-0.002 mg/I. In tha air of warehouse localtons D was 0.0003-0.002 mg/1. When eottm was dieted with DDT from tractors, me eoncondition of U in the nlr ol Ui warning son* na O.OC*-0.04 mg/1. Ninety persons were examined before toe beginning of the field work season when the chemical poisons were used and 33 were examined diriig the sansan (S of Ihnm were working with chemlal poisons tnr toe 1st time and the remainder had rposure histories from 1 to more thts 3 yr). Before tbs beginning of work organophoepfevus tosoctleldes were sot found In the blood of those examined. They were found in the blood ol 90% of them dtrtog the working season. A decrease warn noted In the cerum cholinesterase and alanine aminotransferase. Tbsrs was also a decrease to the ssrum albumins and as Increase to-the a* and gaum* globulin* and to tha albumin-to-flatwlia ratio, to soma of those axamtned tha thymol test was poaXtve. These results are evaluated as to* result of a disturbance of liver fanetIon. --a. r. 43943. PORROVSKD, V. A., A. & PAUBTOV, and 8. V. IVANOV. O pereametre i utochenU predel'no dopuatimylto kantaentrntsll benxola, toluol*. etUbouola t dletllbstaela v vosdikhe prolsvodatvnnykh pomashchenli. {Review and refinement ol the nauttmuw allowable concentration* ol benzene,toluene, etavlbesiens. and dletliTlbsnaene In the air of IntemtrLiTlocailDnl.l TR VORONEZH MED INST 75. 13-13. IBM. translated iron KBT ZH OTD VYP FARMAZOL miMIOTER SREDSTVA TOKSKOU 1949, No. 4.54.883.--Prom the Itteratur# and experimental data mi tha tmdcabyglanie estimation of aronatic hydrocarbons, rediction of the maximum allowable coocen tralIon In the air of todiatrtal locations la recommended as follows: bsnzene, lrom 30 to 5 mg/m^ and toluene trom 50 to 10. The maximum allowable concentration for ethylbenzene aa 6 is(/inl and for diethyl- benzene 10 mg/m* remains toe aaae. When eitlmates are made of the tmcle action of tha substances ol a stogie homologous series, methods should bo used which are adequate for tote group of oubslancv* and they should be seed to all tha laboratcrlee developing tha amxlnim allowable concentrations. While their value Is being decided, there should be 'Ulgatory conslderstlcn of toe clinical material. 43944. PARAMONCBDC. V. M., and V. L PLATONOVA. O tenktsloal1- nom eostoyanll pechanl I thsludm u Iks, pedver^y*fltcbUdlsya vosdeistvtyu khlerorpuUchtaklkh yadohhimilmtsv. {Functional stats at toe liver and stomach to Deceit, affected bv ooiawg.'r^iaHiwgrsaSsC W: ebesaieal ifaiaap dies* eovarad toactlwml steis sf lbs Hvar and to TO persons handitng organoehloroua compounds (DDT, hoxschleraas, ether sulfonnts). Persons with n relatively abort ear? tee record (up t to yr) had Increased addle and pepsinogenous gastric functions and lightly pronounced disorders of the liver function, inhibition ofthe acidic and pepsinogenous gaslrle Unction*, along with s marked derangement of the proteldtoo, arbohydrale, plgmenlsl, antklcaic sto aocrotery haotton of too liver occurred In peeaons with a leg service record (tn excess of 10 yr) exposed to effects of organoehlorais compounds.--C. M. M. 43949. V\zm, A. H., end E. L PLOKHOVA. K aosdhnlyu ekspenwsatai'sot modell "tokslcheskogo anglcncvroz*-, rosnBcajushcheKOMI khronlchenkom voxdelntvll ns orgxatzm parov vtotlkhlcrhto. {Creme of sn experimental model of "toxic swtlcneurosis" dcveloolnc irom iC^ chronic sctl<n ol vinyl chloridevapora^n tn cnmninm.1 OToTi^ ~~ PROF ZABOL 12M: 4f-49. 1968.--In rabblls the chronic action of vinyl chloride vftpora at concentrations ol 9-10 mg/HUr slier* tee rat* of csrdiBB contracttoes, disturbs the electrics] end mectoudcs) activity of the heart, chasgoa the level of imxlmam arterial blood pressure and eauaes a reduction to tha rata of linear blood flow. The changes described to ths tonetlcns of the cardiovascular system la tee xperimsntol rabbits indloite that the modal of the pathology which develops Iron the ehrcmle settoo of vinyl chloride on an organism <aa b* ssetel ter Studying questions of the pathogenesis and exportmsaui therapy of Iotic angioneuroeto. 43948. GRATSIANSKAYA. L. N.r and E. N. SOLOV'EVA. Prlehtor dlliel'noi iavsUdaoatl prl khronicheakikb profeMtonsl'nykh Udokslksisi. yakh. {fleasone for prolonged Invalidism tearing chronic occupattoml Intentcation, j GIG TRPROFZABOL iTOF Wn371IHlL Enl. ** um.j-'Case histories cf 187 persons ineapaeltatsd by chronic p<ii>tiVn wRh Mn, ptaollno, benson* substances Sod pb war* analyzed (slartlag teem the beginning of tovaJldtem). nw follow-ap period averaged (.5 yr. Investigations ahowed paratstonee of clinical msnUesuttOM d poisoning despite discontinuation of any exposure to to* effects of tmle substances. Causes responsible tor to* continued taeapacitation of IhdMduals trained and untrained for new occupations were analyzed. A medical appreach to frosting these 2 groups of patients was suggested --G.M.1L 43947. APEL' mu A. Matsrlaly k tokslkologll nekotarykh promeztole. chnykh produktow prl prolsvodstve tooprenovogo Imuchuka. linfm-TMtu. on the toxicology of soms lnterraedlste prodicts in the pratoetlon of * toopren* rubber.I 8B NAUCH TR KUIBYSHEV MAuCH3&LgffpiW EPIDEMIOL G10 5. 8T-88. 1998. Prom: HEP ZH OTD VYP FARKAtCOL KHIMIOTER SREDSTVA TOK5KOL, 1969, No. 1.54.885. 43943. TILLER, J. R., R. B. P. SCKILLDfO. and J. V. MORRIS. (London Sob. Hyg. aad Trcp. Mod., London, EngL> DX.) toclc motor In mortality Worn cotoisut heart disease. 4(5623]: 407-411, Ulus, 196B.--Between 1933 and 1S82 42% of 233 deaths of nmle workers exposed to cxrbcn dtsuUlito la 3 vucoso raycn factcrle* to England and Wales were eoKUtsd to coronary heart disease'-compared with 24% of tha dmths to tha ether workers of U11 same age, 11% of the deaths to other local man, sad 14% to the Registrar Ccosrat's Table*. Of men wtUi more than 10 yr to the rayoo Ind:stry employed to 1 ol the fsetvlea, those exposed to mrbon dteulflda had death rates from coronary heart disease between 1990 ui 1964 2 1/2 tin** that ol the other workers. This evlttenco of an occupational risk of coronary heart dlsmss from long-term exposure to low concentrations of CSg wan strongest ip ths 1940s sad slight in 1968-82; it may relate to wartime plant conditions. Currant end page ttr* biochemical and morbidity surreys of exposed workers are now needed. These may also throw light on general issues of uhcroaeltre* ato and corccmry heart disease. --J. W. S. 43949. ZABVA, O. N., L P. ULANOVA (tost DML Hyg. Oeeup. Dl*.. Acad Med. SeL USSR. Moscow, USSR.), and L. A. DUEVA. MaurlUy k peresmotru predeTno dopaatlmol kontesntrateii lormal'de glda v vosdikhe prolzvodstvenaykh pomsshdiealL FMalerlals for a reexanliatlon of the wtexlmal permtealble concentratlpn of fornaidehyd* in uw air of industrial establishments.! GIG TR PROP ZABOL Uji): n^o.' liou. (Engl. sum.|--in a concentration of 9 mg/cuhle m. foratoldehyds produces general Icede and Irritative effect! aad sosie maatfeetatioAB of ssuBUtoattoci. Clinical and hygienic observations mad* ton number of woodworking and tsxtUs industries demonstrated that oa tha lore! of 3 mg/eublc m formaldehyde provoked trrttatloa ol the upper respiratory tract and Increased the incidence of dtoaae among worker*. The maximal permissible concentration at fcznwldchT* hcald be rediced to 9.5 mg/eufiie na--c. M. M, 43950. LOPEZ-AREAL DEL AMa LUB (SanaL Antltsbere. toSuL. Bilbao, Spate.), and ISAAC PERKANDBZ MARTIN CBtAHTZa Kcune* ccnlosln asudotamersl por tolea y esotte. IPseudofamored naeiaocealbr sis bv talc and haolin.l ENPBRMTQtAX lf(Wk JH-4W. Bios. Tnnfmni lkftl)J~ ri ratnnsorimlnsis to a 94 yr old nbtM werfeer vu discovered star ha bad worked to the rvbbsr totoiftry for 10 yr. Radlelogfcal nsttofsstoftoaa Intodid plsurdl itkhelsr to* toaffersg* AP00008653 TOXICOLOGY 3B12J .atisa ,0 (| tinmen, inwM, only ett|M tendency cf tad <dapaelly euapeaalon, hydrephoMe. precipitating silicic acid 0 cmmi large dun concentrations in the tn or open handling. jgg require* certain protective n(UWM.J. W. 8. BAGNOVA, M. D., and *. V. TB PLYAKOVA. (F. r. naaaa Moscow Rea. ImI. Kn., Kokov, USSR.) Prom**tike pro. rmMoMil'nyMk caboltvanil koehl v saloviyakh proiavodatvs norykh kitalfbeeklkh vesteheetv. I Prevention o< occutettcnal diseases of ,uiMa in the KedMtlw or new enetnleal niBMiKti.| kun med ISd): I14.T1B. INI. [Engl. $.)-- chemical nmlMcta, Mrllre end amlnee, are derivatives cf fatty adds, the phyelee- cktadcsl properties of which are atilt not adequately studied. Accord* (i0 to the literature antnes exert a marked effect on the skin, irritate ike mneous membranee, la particular the eyes and tipper respiratory tioct, aad are endoeod with a treat tcaclclty. Worker* [111] were (UsOMd at a plant where faWsubetttutes were synthesised. In 16.8% <ca*eo occupational diseases of the skin were found, hi industrial caedMioas the beat treatment was achieved with paate an a eoap basis. jblyetlpleM film ta the bast material for the manufacture of pro tective gloves.--C. K. M. Hit). ANTCNYU2RENK0, V. A. Oprofeaslonal'aol latakaUeatsll vtntWtlorldom. (Occupational poisoning by vinyl chloride.! <30 HI PROP ZABOb-1X91?" BFSI.' XMk--xprrimema were conducted m fSken who were severely poisoned by vinyl chloride. Only the mukfHtittoa of the stage I of poisoning, was completely reversible. BcftnAhW with stage n, the ma]ority of poisoning manifestations yielded poorly to treatment. In a number of cases, the poisoning wsa i progreestvo proeeae, notwithstanding the cessation of the contact oflkths lode substance nod intensive treatment.--Auth. aura. traneL )I1U, COMBA, G. (Inst. Xae. Mod. fiogur. Trab., Madrid, Spain.) Aportsctows at estudio do la incapacidad laboral en loa nsumocenldUees. KHvdv cf work disability in meumocoidoels.1 MED 5ECUR THAB llUlNUJ IKSI/UJ: 93-fK Uluf.1 'Warded. 1868). |Ei*l., Fr. tM Cer. *uo.]--In the absence of substantial changes la breathing espetty, ergoaplromstry la as indtapenslbls technique tor determinlpg welt dlsaMllty. The eorrststtos between working ability aad chargee latte static and dynamic valuta cf maximum breathing capacity was itaAei by determination cf the various cardiopulmonary parameters * -4u the stable etate of the overload. The results are presented. --J, w. a. lim. GABOR, 8., T. FRITS, and Z. ANCA. (InatU. Hyg., Chi), `in.) Contrlbetton a 1'etude du metabotieme lipldlque dee poumcxi* jM a elUcoee experimented, ehes le rat. (The study of the bptd tMshnHeiu in rat ltmm dnrinr nxoerlmentalsiueoaie.i akuh uAL H1BP Mill) TMVTEtTuirEOC , if778fc~llg,-47a. HKis. 1868--Rat* vMeh were saade MUcotle by intratracheal lasttUattoa of 4<hag of emrts showed changes la the ensymallc activity during silica Instillation. Acte ttpsee after sr telHal decrease shows an Increased lipolytic activity ten* the stage of eetabUthed alUeoels. la the tame way, tbs Upopeutifcbpase shew* increased activity during established silicosis | lavim presented oscillations in tas early stages of the experiments! Asue). As lor the pulmonary aoonltaao, its activity decreases. Tte rets of free fatty acids in the pulmonary tissue stays appreciably mtbsI; on tte other hand there |e u noticeable deerease of these Kite la tte blood sernm throughout tte evolution of thi sIllcosls-H. C. TETERINART TOXICOLOGY 21128. CLARKE, E. G. C. (Roy. VM. CM., Lendex, Engl., UK.) tawrttta la tte nit Tvevtewl. BMT VET J 145(d)v U^tB. T 21116. m8, G. F., G. 8. MARROW, and C. H. GORDON. lAMn. Hash. Rea. Dlv., Beitsviue, Md., USA.) Comparative excream sed retention of DDT analogs by dairy cower* J DAIRY BUI Jjfllt iwo-IBGS. Ulus, 186S.--Three iBwupTof 8 cows each were tell wg of p,p'-DDT, p,p'.DDD, or p,p'-DOE per day for SO daya. CwMBtrations of tte compounds in milk fat approached, but did not rtseh, equilibrium during the fcvdlt^ period. From 40 to 00 days, 228% cf the p,p'.DDE, 7.6% of ths p.p'.DCD, and 8.1% of tte p,p'.EM2T MPiP'.EfJT (2.1%)and p,p'.DDD (3.0%) were excreted in the milk. tettte teedlnf of the coapounda ended at 80 daya, the beeline la Ik M cooeetgrattons of all compounds could be described as the tedl let-order terms. The initial partial emeentrations of tte ta ba were 0.41, Ml, and 0.41 with rales of decline of ST, 41, and 211 per day for p,p'-DOT, p,p'-DDD, andp.p'-DDE, reapeettvely. 'MMil pmial aeueentrtttees of tte 2nd term wore D.U, O.M, to, with rates of decline of 1.8, 2.6, and 1.8% per day, respectively. *117. WORD, J. St., U C. MARTIN, D. L. WILLIAMS, E. I. 1LUAM8. R. J. RANCHRA, T. E. NELSON, and A. O. 1TLLMAN. tep. Artm. Set.. Gkto. Univ.. atutwaier. Chia,, USA.) Uraa tnxlelty taftH P tte bovine. JANTMICI 38(8): 788-781. niue. lMB^- Weny was produced in pregaam cows when dosed by drench wllh urea at a level of 0.44 gmAg body weight. 8ymptome of urea tooctclly appeared within 10 mm. TMs ireaiment proved fatal to ctFwe which had been prevtouily tested and then admlntetered a ?T v/v aohittofl of acelle acid at the rale cf 2 molm of arrtir arid per note of urea. A method was developed for handling piurnant cow* to obtain high Mood NHj-X teveie lor s short period of time but to keep the cows alive. Cow* were fed a poor-quality grass hay up to the time of urea administration. Four hr prior to urea ldmlrtatra. tlon, they were fed grain sorghum at a level of 5 gmAg body Might for a standard weight of 111 kg with adjustment for greater weights being made on the bail# ot W '8kg. Whan acetic acid was given at the rats of 2 molea/mole of urea at IS min and at tte rate ot 1 mote/ mole of urea ISO min after the urea was administered the antmalo survived. Using tins procedure, 20 pregnant cows were treated and only 2 death occurred. Treated cows and their paired controls were kept under regular management conditions tor another U mo- and treatment tad no effect on umber of calves bora, birth weight, weaning weight of tte ealvee, or weight changee and rebreeding performance of the cowe--J. W. 8. t 28121. CARR, 8. B, (Vh. Mytaeh. Inst., Blacksburg, VS., USA.}, and DCSf R. JACOBSOK. Bovina physiological responses to tattc fescue and related conditions iot application m a momssv. ~ J bAIRY skin); nn.llVt.'"lUoe.-- myakoiogicai responses were ob served In S8 male Roiateln ealvee In mostly paired compartaone for the ultimata development of a suitable Woasaay technique tor fescue tenacity. TM eflecta of environmental temperature, level of tsod Intake, orgotamino tartrate, aad extracts of both toxic fescue and non* tnlc orchardfrass oa skin temperature cf the distal portion of the tail were recorded. Tte 80% sthanol extract from l<mlc fescue caused a significant deerease Id tall temperature, whereas nontoxic orchard, graae did art. Some differences in tear! and respiration rates occurred. Multiple correlation and regression analysis indicated tiat room tea. perature and level of feed intake explained 57% of tte variation in dally mans akin temperature of the tails of tte animals in n room wttk continuous air movement. Complete removal of food from tte animals for 2 meals before obtaining measurements also caused reduced tail temperature, heart rata, and respiration rata. The moat consider* response differences between presumably lode and naaime extracts were obtained the day of treatment far intraparttoneal administration and the day following treatment for oral admtniatnlioa. Iba assay procedure described employing skin temperature appears to be tte moat discriminating tor detecting reduced blood flow resulting from tte Bdmimetratlan cf extracts of tic toscuo. t 28128. MOUNT, DONALD 1., sad CHARLES F.8TEFRAN. (Nat. Water Contr. Lab., Duluth, Minn.. USA.) Chronic toxicity of copper to the teitead minnow (Mmephafee promote*I la soft water, j rum nEb 6twuu> GAN SBlsji 2448.24SI lSM'.-.Tta mkjtinnim acceptable toxicant ctmeewtraUoa of copper for tte fathead mlsnow (Ptmestales pconulas, Rafineeque) la wager having an BDTA hardness ot 30 mg/Uiterias 1'at.c^} was found to be between 0.12 aad 0.22 of tbs 28-hr TL_ [noedlaa tolerance Motts] value, using survival, growth, and reproduction to evaltmte effect in an earlier study tte application teeter for copper la water with an KDTA hardness of 200 mg/lfter (as CaCOj) was found tabs between 0.08 and 0.08 for the asm* epeetee. Suggaadonx an made ttat should Iwersaee tte uecuracyaad precision e< future determinations of application factors. 26130. KRATZER, F. H., D. BANDY, M. WILEY, and A. N. BOOTS. (Agr. Rea. Sera., Albany. Cam.. USA.) Aflatodn eflecta la poultry. FROCSOC EXP BIOL MED 121(4k 13B1T13IM. lMfl--A ayatonbie study wia msda of Urn aftect of graded levels of dietary aflatrain cw ths pcrtormuwa ot Wotiara under atmulaled practieal eendttlons. No advene ellacta were detected when a ration containing 400 ppb aflatwin was fed to Arbof-Aeree brollsr ehleks from 1 day to S wk of ago. At higher levels (800 and 1600 ppb) advene liver eftecte were detected, baud on biochemical and hietologlcal atudlee. By Oiemleal analysts, no evidence of aflatnin was found la ths moat, liver of blood of broilers tod 1600 ppb tor 60 days prior to slaughte'r, nor to tte eggs, meat, liver, or blood of While Leghorn hem fed a ration containing 2700 ppb aflatoxla for a parted cf 48 days. t 36131. STUDIER, EUGENE H., aad A. A. FRESQUEZ. (Biol. Dep., N. Mex. Highlands Unlv., Las Vegan. N. Max.. USA.) Carbon discide. A mechanism of ammsila totcrancB tn mammals. ECOLOGY S0(5h iiius. ivov^.wren guano peu. Tadxrlda brasHlensis. inkals amiMniatealr mixtures, CO] la padalvely'retalned En suffleiem amounU to nemnhM alkali excess resulting from Increased blood ammonia levels. There la ao etenge tn blood COj levels la bouse mice, Iftm muatulua. tittle brows bate, Mvotls hietfurua. are tetermedlate lakiua respect, laaeive COe reUnlto* ta eleurly related to ansmta tolerance in these antaals. t 31132. VAN RAMPEN, RENT R., and LYNN F. JAMES. (Agr. Rea. 8erv., US. Dap. Agr., Logan, Utah, USA.) Acute RalogetM pofsontng ofateem Pathogamaia of leotons. AMER'J VET MU 3(MiUk 1T). ivU. iliua. IUs.--Twelve mahlre aheap were given a letteldme AP00008654 nsiu-ustu TOXICOLOGY, OZNERAL (VoL 4ft] I1J40 lion of tho rate of Iod9 ot lindane trem both leal and gldd* nriKM u Influenced br temperature and hienldlty.--Aulhera. 122362. STRELETS. N. H. K voprom o (intM anftrtt prt xlkogol' nom or'yanrall. [The problem ol (hr cause of dreih in alcoholic dnmkenwM.l TR TjMHUHAIMNST'ISOVKBSH VflACII <9. 154-155.-- TraTTrsw. REF EH OTJ> WP TAKMAKOL KHIMIOTER SRED6TVA TOJOINOl* I66Y, No. (2.94.1098. (Translation)--A description In given of the angletropte edema at th* ftolt oolite and vnilt occurring repeat edly to subject* observed nr nervous atlnulailoit and alcoholic drunk' an***- It la euggeeted (hat the causa of death In alcoholic Intoxication la some persona can be mechanical asphyxia. 123993. TEREKHOV, Yu. A., and E. 8. LEVASHOV. Ekranlrovannsya satrteeehnaya kamera dlya lafc iketoKlchesklkh l flilolegltheakikh laal*4> ovanlya. [A screened chamber lor etpoeure to toxin aubataneea for toxico logical and DhTeiolosicai studies, i tr u r in NAUl!U-lasu^u msf mu PROF ZABOLJ. SH-33S. U^mi]. From: HZFZHOTDVTP FARMAKOL KHIMIOTER 8REDSTVA TOKB1KOU 1198, No. 2.94.728. 129384. TC8TANOV8KAYA, A. A., V. 8. LAPCHENKO, V. E. ROOiemco, v. i. 5VATKOV, and o. ?. EGOROVA. Nakotory* da&nya o kombtnlrevannora dsletvll entesl DOT I khlorclcsa t oetrom ekaperirnente. [Some dais cm th* combined action o( a mixture of DDT and chloroohoa Install* exncrtmerte.i iu Glciana oJtanira. rm* fcyjtten* ot nuffP" (Ion.I Kiev. lSl-IST' UM. From: REF EK OTD TY9 FABMAKOX. KHIMtOTER SRBDBTVA TOXSDCOU 1IN, No. 2.54,994. (TYsnalattoa).THe DDT and cMorophoa mere (teen once, aeparatsly or mixed, Ultra' fastrtcally, lo rata at 1/2 ol the Loso. The rata xere killed 23 min. later when marked symptoms of poleonlnt had developed. There was a ria* in the blood sugar of all the snimaie, especially those (teen chlorophoa, in wMeh it reached 124.4 mg%; there was a reduction ta the Uver glycogen concentration which vu (reateat In rata (Iren chtorophos (1.84%) and least In rats given DDT (2.89%); there tu a reduction In the concentration of lipids, chiefly la the sona fasciculate when the mixture was given; the mixture also decreased th* cholineat- erase activity of the erythroeytse bat lee* thin DDT alone, while ehloro- pboe alone increased lu activity. la an Ute rata histological atvdlss showed generally marked hemodynamic dliordara. The differences la the action of chtoeophoe and DOT (lean separately from die action of the mixture are emphasised. 122309. TCSTANOVSKAYA, A. A.. V. S. LAPCHENK0, V. I. SVATKOV, O. P. EGOROVA, and V. E. GORDIENKO. (Kiev Bea. last Nutr. Hyg., Xiev, USSR.) O kombtrrirovanaom detatvU DOT l khloro* losa. ttofeea t khlorofbsa. TCombtasd action of DDT with ehforophoa and of thtoohos with chloroobos.l VOP RTAN 77(31; 81-73. 1999. [Engl. Atun'l--TAIta were Conducted la albino rata by lnstltiding acute, eubaeote and ehronfe exparlments with peroral Introduetlon of th* poison*. IXfcowxa adopted as an initial dose ta the mixture* takes at a ratio I: 1. With a Mast* Introduction e< the aUetur* eomprlsiag DDT and chlorophos there te an antagonism with prevalent action of ehlero- phos, wheraas la multiple adaUalairatlon, DDT appears predominant. The mixture of tMophoe with chlorophos is more potsnt than each one of these substances taken Individually.--Authors. 122398. VASIN, A. N.. ant E. 1. PLORHOVA. (Iiwt. Ind. Hyr. Oceup. Die., Oor'kt, USSR.) X petogenesu sabotevanlya, Tocnlkayushehego pn khronlcheekom voadilstvll na organism khlcrtstogo rinila. fPalhoTen- eats of a disease, dereleelnc durlnc chronic action of vinyl chlarlde on tfrwgantsm:!" FARIWKOL TOKaEOL 91(2): 382-272. USie.------iBSfl. (Engl. sum.)--T**t were oonAieted is rabbits te study the btoejectrle acttvity of the brain cortex, anterior and posterior groups of the hypothalamus nuclei, along wtth die poise, arterial pressure and circulation rale during chronic paleonlnga ol anlmxtu wtth vinyl chlor ide (In a concentration of 8-10 mg/Uter. Evidence was produced shew tng that, parallel with changes in the bioelectric activity (appearance of d-rhythmswttha frequency el about to per/aee, rising mean bioelectric acttvtty of th* anterior sad posterior groups of the hypothalamus nuclst) there appeared also changes Is the circulation functions under study. It it Inferred that tn th* Bechantems underlying the development of pathology secondary to a chronic effect oi vinyl chloride a definite rote l* played by tn* fuaeuoaaJ condition of the hypothalamus.--AmhorA 122387. VINOKUROVA, M. K., and 8. A. STEPANOV. Toksikologleheeksya kharakiaiistlka gerutalda fchloraalna. [A toatcologlcal charecterIzatten of the herbtcMe chlorssln f2-chloro-4,V-tna-dietnTiaBiino-a ^ irmtncFl In: g'Leiena i tokilkoiodyt pesutaiaov t muilKl otraviemya. [The hygiene and toxicology of pesttetdes and clinical aspaeta of pelserv ing.] Zdorcr-ya.- Kiev. 4. 17S-1TB. 1960. From: REFER OTD VTP FARMAKOL KHHflOTER SREDBTYA TOKSIKOL, 1961, No. 2.54.117. 122380. WEINSTEIN, I. (Vanderbtlt tfmv. Med. Scfa., Nashville, TStm., USA.), L. WILLHTE, H. A. KLAUSNER, and M. HEIMBERG. There- quiremeRt of free fatty acids for th* fatty Hver ot CCU IMoxlcailoe. ~~ R10C 8CKJ EJIP ilTWJ: mue. iwu.--uvera Isolated surgically from aermal aidmale sod from rats IMamsated with ecu vtn perfaaed in vttrg with a medium Into which pednltie aoM was infused canttmmnaly. Uvwre froaa normal rata worn also trsafad with CCl^ B vitro by direct edBHou of Ih* cbbrlnted byttrocartxm Be tM medium. Under these condition*, poisoning with CO4 resulted in la. hlbltton ot nel release of triglyceride by (he Uver Into the perfusate and stmullBiieoas accumulmlton of irtglyeerld# in the liver. Thee* observations support th* hypothesis that (he fifty liver of CCI4 Intoa. lemon results primarily (rom interference with the biochemical md>. anlamo Involved In formation and rslsane of tho triglyceride ta th very low density lipoprotein of tM acrum.*.Authors. 122919. 2YAQRAROVA, 6. A., M. N. KUKUNA, and T.. V. TEPlyak. OVA. (Leningrad Sanll. Hyg. Med. Inst., Leningrad, USSR.) Gt*t. teheakayt I toksikoloRlchesItsya khsraMerlMUcs preparata khlorld ehetvertichitocoammonltvoco 0motarty*. [Hygienic yd tmncoloric festores of an awmontum tetrachloride base compcMnIY~"mC SAtffr 33(S)f If*18, TIM. 1V99. |Zri. sum.j-'ejqwnmenui inveati(Uiia of a poaetble effeet prodoeed by the compound 34 (ammonium tetradilcr. ids bass) on organoleptic propsraes of watsr, the sanitary regime of water bodies and the state of experimental animals la acuta and ehreatt unitary toxteologlo teats showed that concentrations of this composed, occurring In Die wafer as a determent, should be Halted according to u* fleet on the sanitary rectos*, tta rscommended maximum psmtaelbte coaoentfatlon in wafer faodles la act at a level of 0.09 mg/Uier,*.Author FOOD REMOVES, ADETTIVEa AND preservatives (See also Food Technology; "Veterinary Toxicology' under Toxicology, General; "DUlsleetioa and Veetor Control (Includes Festletdeir under Public Health; "feiseetletdes: Chemical and niyalesl Con trol, Apparatus" wader Economic Entomology (include* ArichMdwH 112390. BAYZER, H. (Biol. FbreehungOeterrslchia. Stteksloff, At, Una, Austria.) Hechwete and quantitative Btstlmmung von Chlorcke). Inehlorid In Motegiechea Material. (Detection and quantitative deUrniiu. tlon of chlorocholin chloride in biological material.I HgnAYUM CHFIT M(&);1 isni. iEi\gL"eum.J~A method for Isolation and tuo. tltatlvc deteraalnatloa of mlcrograa amount* of chlorocholin* chloride (CCC) extracted from green leaves, traits, grains, straw, flour, etc. le described. Quaternary aamontum compounds are separated by lea exchanga column chromatography. Isolation of CCC by preparstSte thin laybr chromatography on celhitoee layers Is followed either by a guaatltattv* colorimetric method using iodine reagent or by a aeai- guaatltattve chromaUgraphle aatfaod on cellulose thla Iayers.-.Asttata t 1223BI. BUGG, JOHN C. Jr., JAMES E. HIGGINS, and EMC A. ROBERTSON, Jr. (Humble Oil and Refining Co., New Orleans, La., USA.) cW"rtn*t*d ltveu m the eastern evater fCrassostrm virglniea) from selected areas cf the South Atlantic sno GuH-ot-Mealw. PEiTIC M0H1T J l<yj7 *-i. igi.Oysters were couectee irom eatoaTine areas in Sooth Carolina, Georgia, Florida, Mississippi, Louisiana., awl Texas and analysed for pesticide restates. peeUcldt levels were determined by the electron capture pi chromatography method and were eenllraed by thin layer chromatography and dualcolumn electron capture gac ehromategtaphy. In general, chlorinated pesticides were either not detected or were found at relatively low level* In sample* eoRaeted from (he Atlantic and Gulf Coast area*. Ot a total of 133 samples, 94.7% contained I or more pesticide*; IB.5% contained 2 or more; 81.2% contained 2 or more; 83.9% con tained 4 or more; and 31.9% contained 9 or more. The level cf e*a*Hi> rity ter pesticide residue* was 0.01 ppm. Some correlation was tad between spraying eg*rattens and pesticide levels in Ute oysters. --Aotbm*. t 122392. CASPER, VICTOR L. (Gulf Coast Mar. Health Set. Lafi., Dauphin Island, Ain., USA.) Galveston Bay pesticide study: water end evster samul* analyzed lor oesuew* residues lauowtnr moseolto control pronram.' PtsttC HPmT J 1(2): IMS, Map. purpose cf flue study was to datsnelM tho effeet cf increased pestteldi applications In the Houston area on shellfish and shellfish-growing waters of Gtlvettoe Bay. TM study was conducted during the fall of 1994 following a large-scale mosquito control program In the Houston area. Water and osyter samples were collected la Sept, and Oct. 1984, during and after (he moequlto control operations. Oyster sample* collected in this etody were compared to sample* collected from April to July 1984, prior to the moequlto operation*. Analytes Included determination of levels of BHC-lladnnc, DDE, DDT, dleldria, endrin, hepttehlor, aldrln, ehlordane, heptaehlor epoxide, methmqrehlor, tcuapbene, and TrltMon, Pesticide levels were determined by the use of electron capture gas-llquld chromatog raphy, with thin layer chromatography for confirmation. Pestiddf levels tn both water end oystere were low et all time*. 1b* data indicate little or no inemse In levels doe to the cootref program la Houston.--Author. t 122393. DUGGAN. R. E. (Food nd Drug Admin., Washington, D. C., USA.) Chlorinated eeatlelde residue* in field milk and other Airy prMhKfTffl^gl^^I7JFT=BTTOT^r The findings^on ia,B3B oojecuve eaetpiee of milk and <Mlry product! examined by the u. 8. Food and Drag Administration from domestic and imported left daring th* period July 1, IBM, through June 30, 1989, nr* reported. A majority cf ih* aaasploo cefilMd psettetd* vftoMuftft. XoaMuao of DDT, DDE, IDE, dtaldrtt, heptaehlor opadd*. AP00008655 SOS91 Chamfeaf Abstracts Vet. 69, 1968 NCC 4722 wick. N.J.). C**. / Physiol. Phamaal. 1966, 46(4). 609-18 Issled. Inst. Onkot., Leningrad, USSR). Formakol. Toktifco]. A *eri* of **pta. boa shown that the mechanisms by 1968, 31(3), 364-5 (Russ). Polyethylene polyaraine (750 mg,/ which the metabolism of EtOH occurs ar strongly influenced by kg.) administered s.c. to rats and mice increased the hepatic tiie partial pressures of the O present in the aerating medium. monoamine oxidase and histaminmse activities, decreasing the Fox the most frequently used gas m'uct., 95% O, + 5% CO,, tyramine and histamine levels in the blood and organs of the the EtOH consumption was abnormally large, and proportional poisoned animals. The pathol. disturbances observed in mice to the concn. of EtOH In the perfusate. However, when the and rats with acute polyethylene polyamine poisoning may be system was aerated with 18% Oi + 5% CO, + 77% Ni, the due to the increased oxidative deamination of endogenous amines consumption of BtOH was similar to that found in tne intact with the consequent formation of aldehydes, NH, and HiOi. rat. The perfusate concn. of M*iCO, HOAc, pyruvic acid, and BJJR lactie acid was measured in all opts. When EtOH was con SOSPSw Effect of niseis on phagocytosis In chronic trinitro sumed at these two different rates, increases in the tactic:- toluene poisoning. Fidemskii, B. L.; Chukkhev, B. M.; pyruvic ratio and aeetate levels of the perfusate were noted Mul'menko, A. M. (Perm. Univ., Perm, USSR). Fantmkol. m both types of opts. However, the utilization of glucose Toksiiol. l68, 31(3), 365-6 (Ross). Trinitrotoluene (TNT) by isolated perfused liver was inhibited, and acetone levels in administered orally at 30 rag./kg. daily for 6 days to rats pro creased markedly, when BtOH wasconsumed at abnormally rapid gressively decreased phagocytosis. Niacin administered ehnul- rates, although no effect was noted in the perfusate levels of these taaeously at 1 mg./kg. s.c. prevented this decrease and even in metabolites when BtOH was consumed at normal rates. 27 creased the phagocytic activity ef the leukocytes to 1.5-times the references. RCYD level in control rats. BJJR 50391P Action of betytUam Ions on primary cultures of swine 50S99X Pathogenic effect of ehronie exposure to vinyl chloride cells. Vcgni Tallurf, Maris; Guiggani. Viviana (Univ. Siena, on rabbits. Vasin, A. N.; Plokbova, B. I. (Inst. Gig. Tr. Prof- Siena. Italy). Coryolot* 1967, 20(4), 355-67 (Eng). The xabol., Gorki, USSR). Farmakd. Teksikol. 1963, 31(3), 369- effect of Be"^ poisoning on mitosis and morphology of swine 72 (Russ). In rabbits exposed to vinyl chloride (9-10 mg./l. kidney cell and lymphocyte cultures was studied. Treatment air) for 4 hrs. daily for 5.5 months altered -waves (frequency with BO** had a harmful effect on all the mitotic phases and >80 Hz.) appeared on the electroencephalogram from the an abnormality of chromosomes was frequent. At high concn*. terior hypothalamic nuclei, and the potentials oi the anterior the action of Be** was less pronounced on kidney cells than on and posterior nuclei increased by 18-30 and 70-85%, resp., over lymphocytes. Be** exerts its toxic action by competition control values. Concomitant changes in the cardiovascular sys with Mr** either in the activation of DNA polymerase or in tem (bradycardia, arrhythmia, decreased voltage of the in the binding of DNA to histone*. L. Mirone dividual electrocardiogram peaks or whole complexes, decreased 90S42q Protective effect of ethyl alcohol towards allyi alco duration of systole, increased arterial pressure, and retarded hol-induced hepatic lasioas. Sehwarzmann, V.; Infante, R.; blood flew) also occurred. The functional change* in the an Roisaunier, A.; Caroli, J. (CHU Saint-Antoine, Paris, Fr.). terior and posterior hypothalamic nuclei may have a role In the C. R. Sec. Biel. 1967, 161(12), 2425-9 (Fr). A 90-min. per pathogenesis of toxic anfioneurosis due to vinyl chloride. fusion of isolated rat liver with blood contg. altyl ale. (0.013 ml./ 180 ml.) caused release of laetic dehydrogenase, malic dehy _ BJJR S0600r Brows FK, HI. Administration of high doaet to drogenase, ami glutamic-pyruvic transaminase into the perfusate rattandmice. Grosso, P.; Gaunt, I. F.; Hall, D. E.j Golberg, and a simultaneous depletion of these enzymes from the liver; L.; Batatone, BUzabeth (Brit. Ind. Biel. Res. Assoc., Carshal- ale. dehydrogenase and glutamic dehydrogenase activities ton, Bngl.). Feed Comet. Toxicol. 1968, 6(1), 1-11 (Eng). were not detected in the perfusate end were not decreased in the Acute oral toxicity studies with Brown 7K gave LD* values >2 liver. Biliary secretion was Irreversibly arrested after 18-20 g./kg. and 8 g./kg. in mice and rats, reap. The values for i.p. min. of perfusion, and hepatic lesions occurred. A 20-min. per injection were 1.5-2 g./kg. tit mice and 0.75-1.15 g./kg. in rats. fusion with blood contg. EtOH prior to the treatment with aDyl When repeated daily oral doses ranging from 0.1 to 2 g./kg. were ale. prevented the eniya* riiease, the hepatic lesl:na and the given to rats, a precipitous wt. leu and a vacuolar myopathy effect on biliary secretion, and did not cause the release of elc. of the heart and skeletal muscles accompanied by Upofuscin dehydrogenase or glutamic dehydrogenase activities. BPJF formation occurred with doses of 0JS g./kg. and over. At the 50593r Effect ef ethanol on hepatic metabolism and enzyme highest dose employed (2 g./kg.), there was also hepatic centri- activities. Nelson, Patricia Ann (Indiana Univ., Blooming lobular necrosis and renal tubular degeneration ftetreatrnent ton, Indiana). 1967,104 pp. (Eng). Avail. Univ. Microfilms, with antibiotics reduced the incidence of muscle lesions. Re Aim Arbor, Midi., Order No. 68-7228. Prom Dies. Abitr. B peated i.p. injection did not have any effect on the heart or skeletal 1968, 28(11). 4688. , SNDC, muscle. Only 2 of the 6 components of Brown FK, 2,4-diatnino- 90994s energy-linked calcium transport In liver mitochondria 5-(p-sulfophenylazo)toluene (I) and l,3-diamino-4-(p-sulfo- daring carbon tetrachloride iattxicatxm. C&rafoli, Ernesto; phnyla*o)benzene (II), produced muscle damage after repeated Tioxzo, Roberta (Univ. Modena, Modena, Italy). Exp. Mol. oral doses of 0-5 g./kg. Comparable doses of mixts. contg* the Paikol. 1968,9(1), 131-40 (Eng). In rats intoxicated by the gas other components as well as I or n were considerably less toxie. tric intubation of 0.5 ml. CCh/200 g,, Ca1* concn. in liver mito After daily oral doses of 1 g./kg., muscle lesions were found in chondria Increased ---10-fold within 18-20 hr*. However, i.p. rats, rabbits, and guinea-pigs, but none were seen in mice or injected "Ca was not takes up by hepatic mitochondria in in hamsters. The results suggest that the intestinal micoflora is toxicated rats as rapidly as in normal animals. la vitro, hepatic responsible for the breakdown of the components of Brown FK mitochondria from CO-intoxicated rats did not show increased to toxie products. Variation* between species and between ani Ca1* uptake, but Ca** release induced by oxidative phoaphoryla- mals of the same specie# may be due to qual. and Quant, dif tioc uncouplers was greatly decreased. This suggested that in CCl* intoxication, the balance between ADP phosphorylation and Ca** uptake is shifted in favor of the latter, thus contribut ing to the accumulation of Ca** in mitochondria. DDJN 90593t Protective effects of alpha-tocopherol on the hepato- toxicity of carbon tetrachloride: an electron microscope study. ferences in the intestinal microbial population. RCNP 50601a Brown FK. IV. Cytopathie effects of Brown FK on cardiac and skeletal muscle in the rxt. Grasso, P.; Muir, A.; Golberg. L.: Batstone, Elisabeth (Brit. Ind. Biol. Res. Assoc.. Carsbalton, Engl.). Food Cosinet. Toxicol. 1968. 6(1). 13.-24 (Eng). Administration of 2 or 3 massive (1 g./kg.) oral doses Meldolesi, Jacopo (Inst. Pharmacol., Univ. Milan, Milan, Italy). of Brown FK to rats induced a myopathy in cardiac and skeletal Exp. hfol. Poikol. 1968, 9(1), 141-7 (Eng). The oral administra muscles characterized by multiple vacuoles ~l-2 m in diam. tion of 2.5 ral./kg. CCh dlld. 1:2 with liq. paraffin to rats produced Ultrastmcturally, these consisted of areas of fibriUolyxis, af severe hepatic lesions within 24 hrs. However, liver damage was fecting initially the A-band. Histochem., the myopathy was markedly reduced in rats i.p. injected with the lipid antioxidant, accompanied by a moderate increase in acid phosphatase activity a-tocopherol succinate (125 mg./kg.), before CCU administra and by a loss of phosphorylase activity. Subsequently, complete tion. This suggested that lipid peroxidn. is the primary mecha lysis of the affected fibers ensued. In the heart, lysis was fol nism of CCU hepatotoxicity. 27 references. DDJN lowed by macrophage invasion and fibroblastic proliferation, 30596u Distribution tad toxicity of aliphatic hydrocarbons In and in skeletal muscle, by regeneration. The occurrence of body tissues. Shugncv, B. B. (Yaroslav. Med. Inst., Yaroslavl, Upofuscin in muscle fibers and in macrophages was scanty and USSR). Formakol. Teksikol. 1968, 31(3), 360-3 (Rue). Gas erratic. When Brown FK was given in the diet at a level of 2re, ehromatog. was used to study the distribution of 6 volatile hy fibrillolysis and an increase in the no. and electron d. of lyso- drocarbons in the mouse and rat body tissues. The volatile somes was observed ultrastmcturally (hiring week 2-3 of the hydrocarbon content of the brain and parenchymatous organs test. These changes were accompanied by a marked elevation was similar, but the level In the fatty tissue was significantly of histochem. demonstrable acid phosphatase. Progressive higher than in the brain, liver, kidneys, or spleen. Then was a deposition of lipofuscin was the principal pathological feature parallel between the toxieity and effective cerebral concn. of iso- during week 3-12. initial damage to the A- and I-bands in both prene, divinyl, and isobutylene, but not of butane, 2-mcthylpent- cardiac and skeletal muscle distinguishes the Drown FK myop 1-eue, or 2-methylpcnt-2-cne. The divinyi concn. was higher in athy from that known to be produced by high doses of corti the medulla oblongata than in the cerebellum or cerebral cortex. costeroids, thyroxine, clilornqumo. or plusmocid, from ischemic DJJK domugc cuul from muxli* clntuunv resulting from deprivation of SOSOTv Somv biochemical changes in animals following acute K, Ca, or Mg. Tiic lysosomal change# and accumulation of polyethylene polyamine poisoning. Soloiitiskoya, E. A. (Nauch.- lipofuscin are suggestive of primary lysosomal damage which AP0bb08656 41109 Chemical Abstracts Vol. 71, 1979 Nn 2i* 14--TOXICOLOGY T. ft. TOXtttOOH 41109* Toxicology t Ttajrl chloride. Schottek, Wolfgang (Abt. Arbeitstoxikol., Holzweissig, Ccr.). Chetn. Toch. (Ltip- rig) 1969, 21(11), 709-11 (Oer). A review with 24 rets. The tonicity of vinyl chloride ia animals end humans, and the relation of the chern. structure of the compd. to its toxicity were dis cussed. CCJG 41U0r Microscopically eoatraated heavy metal intoxication. Silver sulfide process, a method for localised hlatochtmical de tect!an of heavy metals in tissues. Haider. Gerhard (Ger.). Mikrokosmos 1949.58(9), 272-8 (Ger). A review of work is pre sented which illustrates the value of the combination of light mi croscopy with hUlochem. techniques far the detection, localiza tion, and identification of Hg, Pb, BJ, Fe, Zn, and Cu in cells and tissues. Highlights of the silver sulfide histochera. procedure are presented along with representative photomicrographs. 6 refs. .... _ _ Nellie G. Dehnboatsl 41111a Development of the hemodynamic, biochemical, and morphologic changes In experimental endotoxin shock. Sten- gert. Krzysztof (Z Zakt, Anestecjoiegii, AM, Lodz, Poland). Postepy Hu. Dosw. 1949, 23(5), 001-59 (Pol). A review ia given of methods for detg. hemodynamic, biochera., and mor- phol. changes, the course of changes in dogs, clin. evaluation and patterns, and damage to tissuesand organisms during exptl. endo toxin shock. 146 refs. Y. Pomerans 41112t Saponins. Birk, Yehudith (Hebrew Univ., Re- hovoth, Israel). Toxic Const. Plant Focdst. i960, 169-210 (Rng). Edited by Liener, Irvin B. Acad. Press: New York, N.Y. Dietary source, metabolism, and effects of various taponins are given with methods for qual. and quant, analyses. 167 reft. E. A. Hodgdon 41113u Chromatographic identification of psychotropic drugs. Phillips, Geoffrey F.; Gardiner, Jane (Lab. Govt. Chem., Lon don, Engl.). J. Pham. Pharmacol. 1969, 21(12), 793-807 (Beg). The thin-layer chromatog. of 3 classes or psychotropic drugs, phenethyianuaes, tryptamines, and ergmnes, has been in vestigated. Published methods are reviewed and R/ data, nor malised by a graphical technique, are reported for extensions and modifications of some of these systems. Optimum forensic sort ing procedures are recommended. RCHQ 41114v Estimation of lead in urine by atonic absorption analysis. Taira, Yoshiko. Nippon Eiieikenta Gishikai Zasski 1949, 18(5), 389-91 (Japan). The Pb1+ was extd. into iso-Bu- COMc soln. from 39% NHOH, The recovery rate was 99.9%. The reproducibility of at. absorption measurement was 1.4%. No interference from other ions was noticed. T. L. Chang 4!!lSw Dstsrminatioa of toxic tubstacces th*ir me tabolites ia biological fluids by gas chromatography. I. Trl- ehloroethinol la urine. Sedivec, Vaclav; Flelt, Jan (Uatav Hyg. Brace, Prague, Czech.). Prac. Ltk. 1999,21(7), 301-8 (Czech). See CA 70:113446p. VNJZ 4U16x AflatoxinBi in the excretion of aflatoxin-poisoned rats. Chou, Ming-Wu; Tong, Ta-Cheng (Coll. Med., Nat. Taiwan Uuir., Taipei, Taiwan). Tai-Wan J Hstteh Hut Tta Cktk 1949,68(8), 389-91 (Eng). Aflatoxin B( was detected in the rat urine and teces after i.p. inlection of the toxin. Most of the abtaxin Bi was excreted in the first 24 hr. The percentage re covery was about 1.5%. A thin-layer chromatographic method was a simple way for detg. the aflatoxin eonen. of the excretions, and could provide useful information for detecting it in humans. N. M. Wright 4U17y Paper chromatography of Taxus baccate toxin. Bubieo, Zenon (Wyzsza Ssk. Kola., Wroclaw, Poland). Zest. Nauk. ll'yttt. Set. Rain. Wroclaw*. Wot. 1948, No. 33, 215-31 (Pol). The presence of toxin in T. hactata (English yew) needles, in fodder, and in the alimentary tract content was detected by paper chromatog. The alkaloid was extd. from tha biol. ma terial by EttO, purified with active C, and chromatographed is the form of aq. HC1 soln. The chromatograms were developed at 18* by the ascending technique, using as solvent either BuOH- 80% AcOH-aahyd. EtOH-H,0 (80:7:2:15), or 75% aq. <NH)r SO,. The R/ values were 0.96 and 0.75, resp. The toxin spots were stained by the Dragyendarf reagent. Irena Klocslco 41118s Errors of converting a urine alcohol value into a blood alcohol level. Kaye. Sidney; Cardona, Eduardo (Sch. Med., Univ. Puerto Rico, Rio Piedras, P.R.). A mcr. J, Clin. Pathol. 1949, 22(5), 5, <-84 (Eng). Evidence is presented for the In advisability of caleg. blood EtOH levels on the basis of urine BtOH content. EtOH content was detd. in the blood and urine of 148 patients end the ratio of urine/blood was catcd. The range of ratios was 0.21-2.66 with a mean ratio of 1.28. Blood levels were ealed. front urine lewis using a conversion factor of 1.28 and compared with measured values. Caicd. values ex ceeded actual values by at least 0.1)2 g/ml in 21.5% of the cases and were lower by the *amc aim. in 34.6% of the cases. J. R. Macnab 41119a Ethylene glycol toxicity in the monkey. Roberts, James A.; Seibold, H. R. (Tulane Univ., Covington, La.). Tetieol, Appl. Pharmacol. 1969, 16(3), 624-31 (Bag). The toxic effects of ethylene glycol in several macaque species arc re ported. The compd. was administered in the drinking water at concns. of 0.25-10%, and histol. studies were made after acute and chronic administration. With the exception of a few animals (which had Ca oxalate crystals in the brain), significant pathol. alterations were limited to the kidneys. Animals receiving 15 ml/kg or more of ethylene glycol had Ca oxaiate crystals within proximal renal tubules end assoed, tubular degeneration. De- spite the absence of crystals in animals receiving <15ral/kg, mild glomerular damage was found, and azotemia occurred in some animals, suggesting a toxic effect of ethylene glycol apart from its conversion to oxalic acid. RCZB 41l20u A cllnlcopathologic study of tha effects of riot control agents on monkeys. IV. o-Chlorobonxylideae malonoaitriU SIS) grenade. Striker. G. E.; Strtett, C. S.; Ford, D. F.; erman, L. H.; Helland, D. R. (Edgewood Arsenal, Md.). U~S. Clearinghouse Pod. Sci. Took. Inform., AD 1947, AD-808- 732. 39 pp. (Eng), Avail. CFSTI. From V.S. Gnt. Rat. Dt- velop. Rtp. 1949, 69(19). 123. A study was made of the order, severity, and resolution of pathol. changes in monkeys exposed to CS. Monkeys were exposed to CS (2700, 8500, 28,500, or 80,000 mg mm/m1). The most prominent lesions seen after the 2 lower doses were mild pulmonary congestion, bronchorrhea, emphysema, and atelectasis. These lesions cleared by 72 hr but recurred at 1 week and 30 days. Oral and nasal discharges and dyspnea appeared early after a level of 28,500. They were most severe between 12 and 24 hr and were resolved by 72 br. Pneu monia, emphysema, and atelectasis were present 1 week and 30 days after exposure. Significant lesions were seen radiographi cally only in those monkeys exposed to a level of 80,000. These lesions paralleled those seen on necropsy. At this level edema appeared by 12 hr, peaked between 24 and 48 hr, and cleared by 1 week. Emphysema and bronchiolitis were present 1 week ana 30 days after exposure. TCVL 41121v Experimental study on gat embolism with particular reference to the differentiation between embolic gas and gas from putrefaction. Pierucd, Giovanni: Gherson, Gemma (Inst. Med. Legale, Univ. Pavia, Pavia, Italy). Zacehia 1968, (3] 4(3), 347-73 (Ital). Embolism was induced by injecting air or He i.v. into living (followed by instant death), and i.v. or intnwardi- afly into sacrificed, rabbits, and gas was aspirated from the heart at intervals. Putrefaction gas was aspirated from the heart and abdominal cavity of rabbits and human cadavers 1-13 days, and 15-236 days, after death, resp. In samples of air embolic gas, O* decreased and 1 hr after death urns insignificant: CO, was present immediately; N* levels were relatively stable, similar to air, for 2 days, then decreased sharply. In He embolic gas, Os, Ns, and CO, were present immediately. Results were essen tially the same in rabbits injected before and alter death. Av. values for putrefaction gas contents in rabbit and cadaver heart were, resp.: Os 1.32 and 2.05%, Ns (usually <50%) 12.13 and 22.06%, CO, 31.08 and 50.62%; CH appeared early and almost constantly ia the rabbit. F. Famam 41122w Barfy effects of carbon tetrachloride on the synthesis of phospholipids in tha rat liver and their possible pathogenetic role in fatty liver induction. Haibreich, A.; Mager, J. (Ha- dassah Med. Sch., Hebrew Univ,, Jerusalem, Israel). Biockim. Biaphyt. Acta 1949, 187(4), 584-7 (Eng). In rats, i.p. injections of CCL in doses as low as 0.5 ul/100 g reduced the ability of liver microtomes to incorporate choline-,4C into total phospholipids and labeled L-leutioe-2-uC into protein, discernible as early as 10-15 min postinjection and lasting for 220 hr. Incorporation patterns of choline were identical whether the radioactive choline was labeled in the Me groups or In the 1,2-carbon atoms. The magnitude of choline inhibition was not altered by varying the dose of labeled choline over a 1000-fold range, attesting to the independence of this phenomenon of the attendant variations in the internal pool size of free choline. Ethionine (l mg/g) and dimetbylnitrosamiue (10 mg/100 g) did not affect the incorpora tion of choline into liver phospholipids, although they inhibited protein synthesis. CC1, enhanced the incorporation of ethanol- amine-7-" C into liver microsomal phospholipids. The early on- set.and the long persistence, as well as the specific nature, of the de rangement of phospholipid synthesis by CC1, suggest a possible role of this phenomenon in the pathogenesis in (atty liver. BKJN 4112ix In vitro Inhibition of succinate and pyruvate oxidation by ethasolie extraeta of graaaea, legumes, and dystrophegeme forages. Checkc, Peter K.; Oldfield, James E. (Oregon State Univ., Corvallis, Oreg.). Can. J. Anim. Sri. 1969, 40(3), 40:1--1 (Eng). EtOH exts. of title plants inhibited in vitro oxuln. of bath succinate and pyruvate by rat liver humogunuicK. Exts- <>f 2 dystrophogcnic forages did not inhibit succinate uxidn. more than those of two nondystrophogenic samples. The succinic AP00008657 ^g* 17* 14---Toxicology Vol. 7a 1969 1X3464 cnccs in the fatty acid compn. of (he brain between HCN- trcaUid ami untreated rati. N. Constantsa*. 1 U452n Curative tntagonization of liver damage caused by phalloidia with stlymarin as a mode! of antihepatotoxra therapy. Vogel, Guenther; Tcmmc, Inge (11k>1. Inst. Matlaus, Cologne, Ger-). Arznrim.-Forsch. 1509, 19(4), 013-15 (Ger). Silymarln, the antihepatotoxic agent in the seeds of Silybum tnarianum, prevented the toxic effects of phalloidia in mice. Stlymarin was also effective in reversing the toxic effects of phalloidin in mice intoxicated first with this compd. and then treated with sily- tnarin. The antiphalloidin effect of silymarin depended on the time interval between intoxication ana treatment, and on the degree of liver damage. BQTG ll3453p Evidence for the bioactivation of slaframine. Aust, Steven D. (Michigan State Univ., Bast Lansing, Mich.). Bich chem. Pharmacol. 1969, 18(4), 929-32 (Eng). Max. salivary activity in pentobarbital-anesthetized cats occurred ~1 hr. after i.v. administration of slaframine (0.3 mg./kg.); the dura tion of salivation was extremely long, usually lasting for 0 hrs. after a angle dose. In addn. to salivation, most of the exocrine glands were specifically stimulated by slaframine. Upon ad ministration of slaframine to cows, sheep, and goats with pan creatic cannulas there was also a substantial delay before as in crease in pancreatic flow was observed. In mice, a longer delay occurred after i.v. Injection of 2 mg./kg. than with j.p. adminis tration at the same dose; this delay wagedecreased by injection directly into the portal vein. Delay of the effect of the drug at 4 mg./kg. was significantly extended by briefly isolating (with clamps) the liver of rats; the effect was completely eliminated by the permanent isolation of tbe liver. Common inducers of liver drug-metabolising enzymes consistently reduced the delay in the induction of salivation in mice, while inhibitors of the en zymes consistently lengthened tbe delay. Thus, slaframine seems to require activation by the liver before stimulating exo crine glands. The enzymes involved in this activation are probably those responsible far the metabolism of most xatto- biotict. BYJN U3454(i Metabolism of phospholipids in the brain and liver of rata during intoxication with organophosphorus compounds. Dvorkin. V. Ye.; Tofilo, A. P. (Pavlov Inst. Physiol., Lenin grad, USSR). ByuU. Eksp. Bid. Med. 1900,67(3). 50-2 (Russ). Organophosphorus compds. LG-63 (5 mg./kg.) and GA-81 (0.4 mg./kg.) administered i.m. to rats did sot affect the level of phospholipids in the brain and liver or the rate of restoration of phospholipids in the brain. The metabolism of phospholipids in the liver was increased 24.8% by organophosphorus compd. LG-63 and 32.4% by GA-81. The increased intensity of phos pholipid metabolism may be connected in some way to increased activity of the hepatic cells during detoxication of the Cholin esterase inhibitors. BJJR llMSSr Toxicity studies ia mica treated with 1-p-p-mtabltto- fomsBeylcytoalse (ara-C). Leach, William B.; Laster, W. Russell, Jr.; Mayo, Joseph G.; Griswold, Daniel P., Jr.; Schabel, Prank M., Jr. (Med. Center, Univ. of Alabama, Birmingham, Ala.). Cancer Ret. 1960, 26(3), 529-35 (Eng). Optimum therapeutic dosage schedules of l-jS--aribinofurano- sylcytosine (ara-C) administered i.p. in mice (15 mg./kg., every 3 hrs. for 24 hrs.) result in karyorrhectic damage to the crypt epithelial cells of the intestinal mucosa. This damage is most severe at 4 hrs. (earliest observations made) after the end of the 24-hr. course, after which there is a rapid recovery to completion in 72 hrs. The damage is thus transitory and resembles that of hydroxyurea. This cycle of transitory intestinal damage fol lowed by recovery occurs after each therapeutic dosage schedule of ara-C as long as the schedule is not repeated more often than every 4th day. Progressive damage to the intestinal mucosa accompanied by changes in the hematopoietic tissues occurs only if this optimum therapeutic dose (16 mg./kg., every 9 hrs.) is continued uninterrupted to the LDm dose (300 mg./kg. in ~i hrs.) and beyond. Irreversible changes are seen hi the in testinal mucosa, and a progressive depopulation of the bone marrow with ultimate aplasia ensues as the LDiu dose is reached and surpassed. RCTT 119456a Effect of butylated hydroxytolusno (BHT) on rat liver cytochrome oxidase activity. Pascal. Gerard; Terroine, Thcirese (Centre Rech. Nutr., C.N.R.S., Bellevue, Fr.). C. R. Afed.Sci., Paris, Str. D 1969,268(11), 1526-31 (FT). The oral administration of BHT (0.05% in the food, a coucn. 50-fold greater than that authorized for human food) for 8 weeks caused a 13.4-22.4% decrease in hepatic cytochrome oxidase activity in rati of both sexes. The addn. of BHT to rat liver homoge nates at the level of 10 mg./g. Uver caused a 12% decrease in cytochrome oxidase activity. Thus, BHT appears to exert an antioxidant effect an in vivo metabolism. DFJF U34371 Activity of guanine deaminase in rat kidney, liver, sod blood during experimental uranyl nitrate intoxicadoi). l*aidet Janine; Bastide, Pierre (Fae. Mixte Med. Pharm., Llcrmont-Ferrand, Pr.). C. R, Sot. Biol, 1968, 102(5-0), U09-71 (Fr). Guanine deaminase activity (I) was studied in the liver, kidney, cad blood of rats during exptl. intoxication with uranyl nitrate (1 tnl. i.p. of a soln. corn*. 2 g./t. per 100 tt. hotly wt. >, As early as L'-l Iim. later a priixrcw-ivc elevation Of I was noted in all organs, but then- was a definite lowering of I in tiic kidney (or the first 12 hrs. after tltc wart of intoxication. Dorothy J. Euchanun-Davidsou 1134S3U Excretion ot tetraethylgerreanlum is the rat. Oustrin, J.; Clavel, M. J.; Pilot, G. (Centre Kcch. Toxictes, C.N.R.S.. Toulouse, Fr.). C. R. Bar. Biol, 1968, 162(5-6), 1220-9 (Fr); c(. G. Pitet (1907). Kt<Gc ll), a very resistant eompd. in vitro, is di-xradcd very easily in the rat. Although I has a very high b.p., it is eliminated in large amts, during respira tion. The metabolites of 1 found in rat urine are GeOs or the germanates. The metabolism of 1 is different from that of tetra ethyllead (II) or tetracthyltin (HI). No evidence was found for the formation of tricthylgemanium, which explains the weak toxicity of I compared to ll and 1H. Dorothy J. Bnehanan-Davtdaou 113459V o-Hydroxybetyrate dehydrogenase ictivity of rat kidney homogenate fractions after experimental uranyl nitrate intoxication. Bastide, Janine; Bastide, Pierre (Fac. Mixte Med. Pharm., Clermont-Ferrand, Fr.). C, R. Sot. Biol. 1968, 182(8-6), 1492-3 (Fr). Most of the o-hydroxybutyrate de hydrogenase activity of the rat kidney is found in tne super natant after centrifugation at 24,000 g for 2 hrs. This activity decreased with time duriog exptl. uranyl nitrate poisoning, whereas the contents of protein, DNA, and RNA were relatively low and underwent but little variation. C. W. Ackeraon H9460p Effeet of ethyl bromide on the liver. Karimutlina, N. K.; Gizatullina, A. A. (Ufim. Kauch.-Issled. Inst. Gig. Profzabol., Ufa, USSR). Pormakd. Toksikol. (Moscow) 1969, 32(2), 165-7 (Russ). EtBf fumes (2.4 mg./l.) inhaled by rata and rabbits for 4 hrs. daily for 6 months disrupted the func tional ability of the liver, decreased the hepatic glycogen and fat levels, and prolonged hexenal sleep. Granular dystrophy in the hepatic cells and a decreased RNA level in the cytoplasm were detected. BJJR 113461q Hemopoiesis ia ehronie heliotrine poisoning of Wistar rata. Levin, G. S.; Novachenko, Z. I. (Uzb. Naucb.- Issled. Inst. Gematol. Perellv. Krovi, USSR). Parmahd. Toksikol. (Moscow) 1969, 82(2). 170-1 (Ruts). Heliotrine administered sx. at 3-10 mg./lOO g. once a week for 1-8 months to Wistar rata caused hepatic lesions, hypochromic anemia, severe reticulocytosis, normoblastosit, and leukopenia. Erythro blastic sprout hyperplasia, an increased no. of mitoses, and on increased no. of reticular and esp. plasmatic cells were observed. The observed changes in the blood and bone marrow suggest development of on autoimmune form of hemolytic anemia In response to chronic heliotrine poUiooing. BJJR il3462r Benzene metabolism In the Uver of experimental animals and man. Tiunov. L. A.; Sokolova, T-1.; Bandman, A. L. (USSR). Parmakel. Toksikol. (Moscow) 1069, 32(3), 186-8 (Ruse). Tbe rate of CH metabolism during 24 hrs. of incubation was similar in liver homogenates from humans and rats, more rapid in homogenates from guinea pigs, and slower in rabbit Uver homogenates. Species-sped6c differences seemed to be involved in CiH conversion, since the activities of ary1-4- hydnylase and glucuronyltransferase Increased while the sulfonating system activity did not change in CHrtreated rata ls CHrtreated rabbits the sulfate adenyltnnaferase and aryl salfotransferasa activities decreased during disruption of the sulfonating system. The high rate of C*Hs conversion In guinea pigs may be connected with the relatively high liver catalase activity. BJJR 1134d3s Effect of the tedium salt of adtnoeine triphosphate on experimental cyanide poisoning of rati (mechanism of the antidotal teflon). Mesketi, K. V.; Ganxhara, P. S. (Odeos. Med. Inst-, Odessa, USSR). Pormokol. Toksikol. (Moscow) 1969, 32(2), 214-15 (Run). Ne ATP (0.5 ml. of a 1% soln.) given c. or l.m. to rats 1 hr. prior to administration of the min. lethal dose of NaCN prolonged survival. When given *.c. im mediately after lethal cyanide poisoning it substantially extended the life span of 50% and prevented death in tbe other 50% of the rats. The antidotic effect of ATP may result from its ability to restore respiration by bypassing cyanide-sensitive enzymes. BKJR &113464t Changes in the cardiac activity of rata chronically exposed to vinyl chloride vapors. Vazin, A. N.; Plokhova, E. 1. (Gor'k. Nauch.-Issled. Inst. Gig. Tr- Prof. Zabol., Gonki, USSR), Formakol. Toksikol. (Moscow) 1969, 32(2), 220-2 (Russ). Chronic (5 months) exposure of rats to vinyl chloride vapors at 0.03-0.04 mg./l. disrupted cardiac work rhythm, in duced bradycardia and arrhythmia, and reduced the relative duration of I-1I and T-U sound intervals. The relative dura tion of the Q-T complex did not change significantly. Within 15 days after termination of poisoning, the rat cardiac activity rhythm returned to normal, but the duration of the 1-11 ana T-II sound intervals remained below the initial level for another 15 days. The maximal permissible eonen. of vinyl chloride apparently ia significantly less than the 0.03 mg./l. level pre viously eitd. BJJR AP00008658 r*g* tM 24--Toxicology Vol. 71, 1969 89828 ]>b poisoning aiul inonicHlcirw*ivc iwycliosis arc discussed and die striking MnuWitk's between them iioImI. It is sUK Rented ilmi 1*1* may Ijc ;i mxlc doteriiihiaiit in this psychosis. The Davis test (Davis mill Aiuldmati, 1 t*tlT) was used foe tile iktn. of urinary 6-aminolevullnic acid (1) levels in patients and controls. Result* showed an increasingly significant me in I levels between controls and untreated patients and patients treated with Li*CO. Li may interfere with Hb synthesis in a similar way to Pfa. 41 references. S. M. Maxwell 80616s Effects of DTPA (diethyUnetrUmiaepeataecedc acid) aerosol on lung contamination by lanthanum. Pasquier, Christian; Voisin, Dominique; Thieblemont, Pierre; Perrault, Gerard; Bayard, Jean P. (Dir. Prot. Surete Radiol.. C.E.N., Fontenay-aux-Roaes, Fr.). Comntis. Sngri, At. (Fr,l Rapp. 1969, CEA-R-3733, 19 pp. (Fr). Avail. CEA. By use of DTPA aerosol in the treatment of pulmonary contamination by La, its effectiveness was detd. as a function oktime. Provided that it is administered at an early stage, in the 2 hr*, after expo sure, >50% can be eliminated using lower therapeutic doses than those prescribed for other methods of administration. Its assocn. with some enzymes did not enhance the therapeutic effect of the chelator. The const, for the passage of the DTPA- La complex from the lung to the blood is '"~O.016/min., corre sponding to a period of 44 min. A significant noncheiatable fraction, persisting after prolonged action of the DTPA, of the order of 40-45% Is observed, DWJF 69617t Feme eyanofarrate(H): an effective antidote In thallium polasuing. Heydlauf, Horst (Inst^Strahlenbiol., Kemfonchuagszentrum. Karlsruhe, Ger.). EvKJ, Pharmacol. 1969, 6(3), 340-1 (Eng). The influence of orally administered ferric cyanofemte(II), (Prussian Blue, PB) on the distribution snd excretion of **T1 was studied in the rat. PB lowered the retention of **T1 by inhibition of its absorption and reabsorption from the intestinal tract. The toxieity of TljSO was decreased by PB. PB is suggested as an antidote in Tl poisoning. V. N. Gupta 89618a Cyeloherimide-Induced ultraitractnral changes in the adrenal cortex of the rat. Dzsinich.Cs.; Sxabo, D.; Okroe, I. (Inst. Exp. Med., Hung. Acad. Sei., Budapest, Hung.). Experientia 1969, 35(8), 836-6 (Eng). Wistar rats of both sexes weighing about 180 g. were used. Conspicuous changes in the ultra*tmcuire of the zona fasciculate cell* were observed follow ing decapitation at SO min. even after the lowest (5 mg.) dose. At low-power magnification, compact masses of irregular shape, medium d. and blurred outlines were seen to have accumulated between organelles. In expts. involving both cycloheximlde and ACTH treatment, the changes were similar, although the cells appeared swollen. Similar observations were made in some cells of tbe tone reticularis, but not in the xona gtomeruloaa. The findings suggested a correlation between the substance seen to accumulate among organelles end the increased cholesterol content of the adrenal cortex. The ultraatruetural changes ware the consequence of an Impairment of the easyme system. P. J. Periaek 89619V Effect of acetone on the level of ketone bodies in the blood and urine of experimental animals of different species, Linyucheva. L. A.; Tiunov, L. A.; Kolosova, T. S. (USSR). Formahol, Toksikol. (Afwrote) 1969, 82(4), 465-7 (Russ), Rats and mice exposed to 30 mg. MctCO/1. and rabbits and guinea pigs exposed to 72 mg.A. for 2 hr*, showed increased levels of MeiCO, acetoacetlc acid, and fi-hydroxybutyric acid in their blood and urine immediately after inhalation and 24 hrs. later, in all 4 species the 0-hydroxybutyric acid level increased to a greater extent than did the other ketone bodies. The largest in creases in the MeiCO and acetoacetlc acid levels in tbe blood both immediately and 24 his. after acute inhalation occurred in rats and the smallest changes appeared in rabbits. BJJR 89620p Nature of the aeute motion exerted on the organiaa by manoethanolethylenedlainine. Tikhonova, G. P.; Sidorov, K. K. (Inst. Med.-Biol. Probi., Moscow, USSR). Farmaiol. Toksikol. (Moscow) 1969, 32(4), 473-5 (Russ). Monoethanoi- ethylenediamine (I) injected into the stomach of rats ia a max. tolerable dose (2.0 g./kg. in 30% aq. soln.) disrupted tbe add- base balance in the blood after 3-6 hrs., increased the pH from 7.18-7.33 (control) to 7.4-7.49, and increased the level of free bases. One day after I injection the Ca level in the serum in creased, the time of blood coagulation decreased, the blood pH dropped to 7.11-7.24, and the amt. of free bases was below nor mal. The level of globulins decreased and tbe content of albu mins increased in the blood; the level of chlorides in the urine decreased. I caused necrosis in the mucous membranes of diges tive system organs, probably through itsalk. properties. BJJR 89621a Changes in adrenaline-like substances in rabbit blood following chronic exposure to vinyl chloridefumes. Vasin, . A. N.; Plokbova, E. I. (Inst. Gig. Tr. Frofsabol., Gorki, USSR). Gig. Tr. Prof. Zabol. 1969, 13(6), 4G-7 (Rust). A group of 8 chinchilla rabbits was exposed for 4 hrs. daily, during 6 months, to the vapor* of vinyl chloride (1) in air at 0.02- 0.U3 mg./l. Samples of blood were taken from the ear veins and analyzed for adrenaline (11) and adrenaline-like (11a) substances. Before tire exposure to I anil also in the control group of rabbits the II and lie level* varied little end averaged 3.5 pg.%. Aker 20 day* exposure iHc level <>( II and He ruse to 0.15 MX-'ii. after 40 days it reached 0.(1 pk.' i'i nrul it remained const, thereafter. The biopotential of tltc posterior hypothalamus also changed. These effect* of I are the direct cause of liyiarumiott. CPJR S9622r ATP and lipid contents in the liver of mica aftar in halation of chlorinated hydrocarbons. Ogata, Masane; Tomo- kunl, Kaisuinaro; Watanabc. Slnnsaku (Med. Sell., Okayama Univ., Okayama, Japan).,' fnd. Health {Kavjataki, Jaba.) 1968. 6(3), 116-9 (Eng). The hepatic ATP level decrease and the onset of fatty liver as indicated by the increase in the amt. of the total lipids and triglycerides in the mice exposed to 3 kinds of chlorinated hydrocarbon* are in the following order: CC1, tetrachloroethylene, and trichloroethylene. The total lipid and triglyceride contents increased proportionately to the fall in the ATr level before and after the exposure to 3 kinds of chlorinated hydrocarbons. It was suggested that there is some dose rela tionship between the decreased hepatic ATP levels and the de velopment of fatty liver in chlorinated hydrocarbon poisonings. B. V. Shetty 89428s Influence of subacute intoxication with manganous chloride and carbon tetrachloride on the lethal dote of digitoxln, lanatoalde C, and etrophanthoside in guinea pigs. Lenee, P.; Valentincic-Budihna, Metka (Med. Fee., Ljubljana, Yugo slavia). Jugoslav. Physiol. Pharmacol. Aela 1968, 4(2), 157-64 (Bug). In guinea pigs the toxieity of the title cardiotonic gluco- sides is modified in subacute intoxications with MnCli and CGU (when evident morphological changes are restricted to the liver). The following doses were used: 0.01 ml./kg. CCU In 2% oil sola, and 0.003 g./kg. MnCli in 3% aq. soln., i.m., twice a week, or 10 times successively; glucosides were administered 30-39 days after the last dose of CCU or MnCI,. in intoxicated guinea pigs the lethal dose of digitoztn did not differ significantly from that of controls. The lethal dose of lauatoside C was significantly smaller only in CCU treated animals and the lethal dose of stro- phanthoside was significantly smaller in both intoxicated groups. G. Muaeevic 89624C Antidotes for phosgene-induced pulmonary edema. Boyd, Eldon M. (Queen's Univ., Kingston, Ont.). /. Pkorm. Pharmacol. 1969, 21(8), 557 (Bng). Death rates from phosgene were reduced in rabbits, cats, and rats if the animals were allowed to inhale the phosgene through the nose rather than directly into the trachea. David B. Sabine 89625u Demethylphalloin. Puchiogtr, Herwig; Witlasd, Theodor (Univ. Frankfurt, Frankfurt, Ger.). Justus Litbitt Ahh. Clum. 1969, 725, 238-40 (Gar). Demethylphalloin-*^ (I) was obtained by redo, of the CO group of ketophaDoidln with *H-labeled NaBH,- I bed almost the same toxicity as phaUoidin. During storage I gave a degradation product whose uv spectrum indicated a sulfoxide structeze. BTJG 89626v HUtechexnlcal study of cytochrome oxidase and succinic dehydrogenase activityin encephalon of rabbits poisoned by alcohol. Lo Menzo, G.; Cniara, A. (Univ. Cstania, Catania, Italy). Med. leg. AssUurasieid 1968, 16(3-4), 163-72 (Bag). An increase in both cytochrome oxidase end succinic dehydro genase was observed in the cells of the cerebellar cortex of rabbits which had undergone either chronic or acute ale. poisoning, as compared to tbe nonpoiioned control animals. No change waa evident in other eneepheUe regions. The increase of these en zymes at cerebellar level agrees with the symptomatologies! data indicative of functional damage of the area during ale. poisoning. Murrie W. Burgan 89627w Nicotine degradation in hamsters and rata and its modification by foreign substances. 11. Effect of carbon mon oxide treatment ef the animats on their nicotine metabolism. Harke, Hans P.; Frahm, B.; Schultz, Ch.; DontenwiU/W. (Inst. Wiss. Forscbungstelle Verband, Cigarcttcnind., Ham burg, Ger.). NatUTvisstHsekofUn 1969, 56(8), 418 (Ger). Deg radation of nicotine to eetinlne ia rat and hamster liver homog enates was not affected by prior exposure to CO. GKJG 89628x Toxie effects of calcium, titanium, and niobium borides in rats. Kasparov, A. A.; Zhilova, N. A. (USSR). Nov. Dannye Toksikol. Rtik. Metal. Ikh Soedin. 1967, 162-60 (Russ). From Rtf. 2k., Parmakol., Khimioier. Sredstva, Teisi* kol. 1968, Abstr. No. 7.54.779. LD* of CaB (I), during i.p. administration to rats, was 1.045 mg./kg. Intoxication with I is expressed by depression and disorder in movement coordina tion. Chronic oral administration of I to rabbits (100 mg./kg./- day for 4 months) caused leukocytosis, lymphocytosis, increase in conen. of a-yglobulins and activity of aldolase, and decrease in albumin in blood. Prothrombin in blood at beginning de creased and then increased to the end of expt. In lungs then waa polyetnia of tissues and thickening of interalveolar septuas. In intestines there was sclerosis of all vessels in submucosal layer, decreased glycogen in liver, end accumulation of add mucopolysaccharides in the region of hepatic triad. Moderate intensification of lung pattern was observed by x-ray studies in animals 6-9 months alter single intratraclieal administration to rail of Ti boride (II), Nb boride (III), and esp. of I (60 mg.). AP6b008659 Ptg* 1S1 II--Mrtrn/n.iKnn S/oefiemfslry Vo!. 71, 1969 110S73 nnimals. The major difference lies in the larger maintenance may contain factors, not mre->:irily histones, which affect gene requirement* of llic mouse due to its hh'ltcr im-tnlmlie rule. activity in a iiimincr tluit is tun mhi-Iin. %.|*vit\r. USJN Hull]) Jiii ohson 110500r Changes In the activity <.f jsnh?e,| vascular smooth 110S63J Levels of immunoglobulins in normal muscle in response to reduced oentnlaiit.v. Nm wm, (>lc,i (Univ. ildren. Talxilu. Kilmchiro (Kyoto Univ,, Kyoto, Japan). Goieborg, Gnu-bore. JSwwI.i. 1969,77(1), noniktt fCiy 1005. l'l'.tij, 379-WV fling). Li-vt I- of ;orunj 191-200 CKiijl;'. The ilTi-t* -f by: i.ii.iuriiy on the spyji- immunoglobulin), were deed, by the uinilxxly agar p/alc method m toncuus activity of the smooth muscle m tiio isolated portal vein maternal and cord Wood at the time of delivery and in croups of of the rst have born studied in solus, with varied ionic coinpii. A normal infants and children up to aec 15. y.\ wu absent from comparison between the stimulatory effects of lowered osmolarity eord blood, ->M promt only in traces, whereas the levels of >G and increased |K*|* (external K' eonen.) indicated that at in cord blood were equal to or above tW.c in maternal blood. Minimum levels of yG were found at 3 and ft months, then rose slowly, not attaining the adult range until uge 11. Levels of -yM normal | K l-in the control state the excitation caused by hypo- tnnicity is not mediated only via changes in the JK*)i'|K '| ratio. The rcsixnise to Uypoosmofarity at a higher [K.+| is, however, reached half the ranee found in adults by 3 months, but did not more readily explained by the change in |K*)i/[K*U which reach adult levels until 3 yrx. Levels of -rA were less than half occurs in zssocu. with the swelling of tiie cell*. Thi* might be the adult range up to age 5, then rose rapidly. Jacob Sacks attributed to the fact that the membrane potential of smooth 110564k Polymorphism of transferrins of bovine, porcine, and muscle is more dependent on the traustnembrane concn. gradient canine sen, as determined by means of teryfinude-gel disc for K*athiRherlveUof lK+]. The stimulatory effects of hypo electrophoresis. Domer, Joseph L. (Univ. of Illinois, Urbana, osmolarity are markedly reduced at a low [NV]i but are not III.). 1968,66 pp. (Eng). Avail. Univ. Microfilms, Ann Arbor, considerably affected by comparable redns. in |K+] or (C1"J*. Mich., Order No. 60-10,687. From Diss, Abstr. B 1969, 29(12), This indicates that the transmembrane Na+ gradient is involved 4727. SNDC 110565m HeiaogUbla-Lepore Baltimore, a third type of a Id crossover (9*, 0"). Ostertag, Wolfram; Smith, Ernest Wendell (Sch. of Med., Johns Hopkins Univ., Baltimore, Md.). Eur. J. Biothem. 1969.10(2), 371-6 (Eng). Anew Lepore-type Hb \n a person of Negro descent has been identified. In this person a minor Hb (Hb-Lepare.m^,) was detected. An abnormal 10-chain was isolated and fingerprinted. This hybrid-type pro tein presumably arose as a consequence of unequal crossing over between the I and 0 genes, as was concluded for Hb-Lepores.M and Hb-LeporentittSu> In the new Lepore-type Hb described here the crossover could be placed between residues JH and 0M. This type of crossover should occur with the highest frequency if crossing over is equally frequent between any nucleotides and if selection favors none of the crossover types. RCBY 110566s Genetic and developmental regulation of hepatic J-amioolevuUnst* dobjrdrtUte is mice. Doyle, Darrell j.; Schimke, Robert T. (Sch. of Med., Stanford Uiuv,, Stanford, Calif,). J. Biol. Cktm. 1969, 244(20), 5449-59 (Eng). The mechanism by which the levulinate locus (Lv) regulates the tissue tivity of l-aminolcvullnate dehydratase in micehas been examd. .e assayable hepatic eneyme activity in bomesygous LsP mouse wcrains, represented by C57BI/3, is between one-third and onehalf that of homozygous Lv* strain*. This difference in enzyme activity is due to a difference in amt. of enzyme protein ai demon strated by immunochem. techniques with an antibody specific for l-aminolevulutate dehydratase. Combined immunochem. and isotopic techniques show that the levulinate locus regulate* the concn. of hepatic S-emiaolcvulinate dehydratase by acting at the level of eutyme synthesis. The rate of degradation of hepatic enzyme is the tame in both low and high activity strains; when expressed as a half-life, this is equal to 5-6 days. The pattern of l-aminoievulinate dehydratase development with age is similar in livers of both low and high activity strains. The specific activity of the enzyme is high in fatal liver, decreases during the several days prior to birth, and increases to the adult level during the first 3 weeks of postnatal life. The activity of l-amino- levulinate dehydratase in fetal liver is also regulated by the levulinate locus. The enzyme in fetal liver is approx, twice as active catalytically as the enzyme in adult liver relative to its function as an antigen. The fetal enzyme also appears to be different in stability to beat and proteolytic inactivation. How- ever, fetal and adult enzymes axe similar by other physiochcm. criteria, including electrophoretic mobility, sedimentation coeff., and Jm for the substrate. RCJN 110567p Identification and genetic control of two new lew- density lipoprotein allotypes: phenogrovps at the Lpq locus- Albers, John J.; Dray, Sheldon (Med. Center, Univ. o! Illinois, Chicago,.Ifl.). J. Immunol. 1969, 103(2), 155-62 (Eng). See CA 69: 104403*. VNJZ in the mechanisms by which hypoosmolarity excites the spon taneous activity of vascular smooth muscle. One possible interpretation is that the swelling of the muscle cells in hypotonic wins, causes an increase fn the permeability of the cell membrane to the relatively larger Na+. RCVV 110570J Secretion of rat pancreas perfused with plasma from rats fed soybean trypsin inhibitor. Khayarabashi, H.; Lyman, R. L. (Univ. of California, Berkeley. Calif.). Amer. J. Physiol. 1969,217(3), 646-51 (Eng). When plasma from rats fed soybean trypsin inhibitor was perfused through an isolated, fasted rat pancreas, amylase secretion was 2-3-fold greater than that of a pancreas perfused with plasma from rats fed the same diet without trypsin inhibitor. Adan. of atropine to the perfusate did not inhibit the secretory response. The plasma factor was relatively unstable upon storage at 4* (50% loss of activity in 4 days) and appeared to be assoed. mainly with those plasma proteins of 10,000 mol. wt. or less. Thus, active soybean trypsin inhibitor enhanced the formation and (or) release of a humoral pancreosy- miniike substance that markedly stimulated external enzyme secretion of the rat pancreas. RCDH 110571k Effects of diet and catechol amines n bile add metabolism in dogs. Gant, Joseph H.; Cater, Marilyn R. (Coll, of Med., Univ. of Vermont, Burlington, Vt.). Amer. J. Physiol. 1969, 217(4), 1018-24 (Bng). Five adult male dogs were surgically prepd. with polyvinyl tubing placed in the upper duodenum. Each dog was given cholic-caw*y/-uC acid and samples of gallbladder bDe were withdrawn over a 10-day period. The tauroeholie acid pool was 1.09 g./IO kg. body wt. and halflife wu 3.3 day* when dogs were given a dry dog food diet. Epinephrine in oil, 0.8-1 mg./kg./day, increased plasma cho lesterol concn*., produced a 23.9% redn. in the tauroeholie acid pool size, and increased the rate of decline of specific radioactivity of both tauroeholie and taurodeoxycholic acids. The half-life of taurocholic-^C acid during epinephrine administration was 1.6 days. Norepinephrine m oil, 0.8-1.0 mg. free bass/kg./day, produced a modest decrease in the half-life of tauroeholie-1'^ acid without significant elevations in plasma cholesterol concns. A dietary supplement of */Ib. beef plus 2-3 g. of cholesterol per day resulted In increased plasma cholesterol concns., increases in tbs tauroeholie acid pool size, and a decrease in the half-life of taurocho!lc-l4C acid. Epinephrine administration further in creased plasma cholesterol concns. in cholesterol-fcd dogs, but did not significantly alter bile acid metabolism. RCDH 110572m Effect of osmolarity on canine renal vascular resistance. Gazitua, Sergio; Scott. Jerry Benjamin; Chou, Ching-Chung; Heddy, Francis J. (Michigan State Univ., Bast Lansing, Mich.). Amer. J. Physiol. 1969, 217(4), 1216-23 (Eng). The effects of changes in renal blood osmolarity, pro duced by close intraarterial infusion of solns. of dextrose, NaCl, and urea, on renal resistance were systematically examd. in the dog. , Infusion of a hyperosmotic soin. of dextrose for 3 min. 110568q Modification by credo histones of gene activity for produced a fall in renal vascular resistance which was sustained lactate dehydrogenase. Latner, A. L.; Longstaff, E. (Roy. for the entire infusion period. On termination of the infusion, Victoria Infirmary, Newcastle upon Tyne, Engl.). Nature resistance returned to the control level, infusion of a soln, of (London) 1969, 224(5214), 71-3 (Eng). Addn. of crude calf hyperosmotic NaCl or urea, on the other hand, produced a fall in thymus histone to the mouse kidney organ culture increased the resistance which waned with time. Furthermore, on stopping proportion of the lactate dehydrogenase (LDH) isozymes which the infusion, resistance transiently rose well alxive the control moved more rapidly toward the anode during electrophoresis. level, particularly in the case of urea. Intraarterial infusion of " a crude rat liver histone fraction was added to the culture, the an Isosinotic snln. of urea and of liypnsmotic solns. of all three -ease was in the direction of the slower moving LDH Isozyme*. substances produced large increases in resistance. Studies con .. the cultures exposed to the crude calf thymus histone, the cerning lymphatic vessel pressure and cell-tree perfusion, as welt --percent of LDH-I and the H subunit contribution to the total ax the findings of others, are consistent with the hypotlicsis that LDH activity significantly increased, whereas the opposite was the changes in resistance result, to a large extent, from active found with the rat liver histone prepn.; LDH-5 and the M sub vasomoticn, possibly due to osmotic changes in the water content unit significantly increased. Enrichment of the medium with of the smooth muscle cells. KCDH amino acids present in the calf thymus histone prepn, did not 110573n Metabolism of ally! compounds in th* rat. Clapp, alter the isozyme pattern, indicating that the changes in LDH J. J.; Kaye, C. M.; Young, Leslie tdl. Thoinas*K llosp. Med. pattern were not caused by hydrolysis of the histone. Gene Sch., London, Jitigl.). BtVvW J. 1966,114(1), l-7P (Eng). masking by histones apparently is nonspecific, and the crude ext. A mcrcapturic acid was isolated from the urine of rats treated s.c. AP00008660 110574 Chemical Abstracts Vol. 71, 1969 Page 152 with CH:CMOAc or ClEi:CIIOJl and identified as 3-hydroxy- studied. A dose of 2000 f.U. u-asparaginasc/kg. evoked hyper. * propylrncrcapturic acid (/V-at:etyl-.'i-(3-hy<)roxypropyl)-t.*cys* Ktyccinia and glycosuria in most of the animals; this is equal to a teine, 1). There was no evidence (or the presence of allyltnvr- dose which induced hyperglycemia when Riven to patient*. Ah capturie acid (iV-acetyl^-aHyl-wysteine, II) in the urine of rabbits receiving 10,000 f.U. L-asparaginose/lcg. vr more showed these animals. II, however, was detected in the urine of rats injected s.e. with CHitCUCl. .S'-altylKlutathioiie, and .V-allyl-Lcysteine. Rats treated with CHj:CHC1 also excreted I. Mcr- diabetogenic responses, but these effects were temporary (2-13 days) and were not assoed. with any histological changes in the pancreatic islets, except in 1 rabbit which showed islet hyaliniza- capturic acids apparently arise by the reaction of ally! compels, or tion. DDJN' their active dcrivs. with glutathione, BQJN JlOSSlp Effect of Bidrin on the metabolism of dietary com H0S74p Metabolite* of octoclothepine eliminated in human ponents by tite bovine. Lowrey, R. S.; Bowman, Malcolm C.; and rat urine. Qucisnt-rova, Milena; Svatck, E.; Mctysova, Knox, F, E. (Coastal Plain Exp. Sta., Agr. Res. Scrv., Tifton, Jirtna (Res. Inst. Pliartu. Binchein., I'niRUc, Czech.). Biochem. Ga.). J. Dairy Set. 1969, SJ(9), 1400-3 (Eng). Six steers J. 1949, 114(2), 330--12 (Eng). Four metabolites and unchanged averaging 400 kp. in wt. were used in a switchback trial to study octoclothepine were extd. with diclUoroctlianc from the urine of the effect of spiking corn silage with 15.7 ppm. (0.24 mg./kg. humans given octoclothepine. These substances were isolated body wt.) of the pesticide Bidrin (3-(dimethoxyphusphinyloxy>- and purified by column and thin-layer chromalog. By chro //,N-dimethyl-rf*-crotonamide| on blood cholinesterase, digest matographic, spcctrophotometric, and poiarographic anal., ibility of dietary components, utilization of X, and rumen fermen unchanged octoclothepine and 3 of the metabolites were identified tation. Bidrin residues were not found either in the urine or in (noroctoclothepine, noractoclothepine .''-oxide, and octoclothe the feces of animals ingesting Bidrin. Azodrin. a metabolite of pine i'-oxide). The presence of gluuuronldes in human urine Bidrin, was not present in the feces of treated animals but aver wasshown. The same metabolites and unchanged octoclothepine aged 0.430 ppm. in the urine. Based on urine vol. this repre were also found in rat urine by chromatog. RCh'G sented 1.5% of the ingested Bidrin. Blood cholinesterase was . 110575a Mechanism by which lends* and arginine stimulate depressed to 30% of pretreatraent values by the end of the 42-day Insulin release in vitro. Milner, R. O. G. (Univ. West Indies, expti. period, whereas activity of steers fed untreated silage was Kingston, Jamaica). Bioebim. Biopkys. Ada 1949, 192(1), unaffected. In the metabolism trial conducted during the last 154h9 (Eng). Basal Insulin release in young rabbit pancreas 14 days of the 42-day period Bidrin had no effect on the digest slice* in vitro was higher in the presence of leucine (5mA/) than ibility of dietary components (dry matter, crude protein, ether of arginine {SmAf) (in the absence of glucose). Glucagon (5 ext., crude fiber, N-free ext., and gross energy), tile utilization pg./tnl.), theophylline (1mA/), dibutyryl cyclic AMP (1mA/), or of N, or rumen fermentation. RCLD u-arginine (5m.1/) stimulated insulin release in the presence of I10582q Meetof a second doe* of cadmium salts on vascular leucine but not in the presence of arginine. Arginine-stimulated permeability in the rat testis. Clegg. E. J.: Can, Ian; lViemi. basal insulin release perhaps works by releasing glucagon from M. (Univ. Sheffield, Sheffield, Engl.]. J. Endocrinol. 1969, 45 the -cclls. The mechanism of the leucine-induced stimulation (2), 265-8 (Eng), Two doses of CdClj were given to rats at is different, probably via the adenyl cyclase system. BSJN intervals varying from 76 to 194 days. After the second dose the U0576r Cytocbemlctl study of the distribution and content of blood vessels In the testis and initial segment of the epididymis naeleoproteins sad of tome functional groups of proteins in iso showed increased permeability, as demonstrated by i.v. markers. lated formations of the central nervous system following foreign In the testis, leaking vessels were usually found m areas where protein injection. Tumanyan, E. L. (Erevan. Med. Inst., interstitial cells were abundant. RCKV Erevan, USSR). Biol. Zb. Arm. 1949, 22(55, 97-8 (Russ). U0583r Add-base and electrolyte changes induced by acute Cytoplasm of various parts of rabbit central nervous system Isotonic saline infusion In the aephrectomizad dog. Rosenbaum. (including intervertebral ganglia, nuclei of the XII cranial nerve, Barry J.; Makoff, Dwight L.; Maxwell, Morton H.; Hogh- and skin motor area of brain cortex) displayed higher ribonucloo- oughi, Michael (Cedars-Sinai Mtd. Res. Inst., Los Angeles. protein content in animals treated with repented i.v. injection of Calif.). J. Lab. Clin. Mad. 1969, 74(3). 427-35 (Eng). The normal horse serum. The peek effect was observed on the 4th mechanism and extent of "dilution acidosis" by acute infusions day alter the last injection. N > changes were seen in content of isotonic saline was studied in nephreetonmed dogs. Infusions and distribution of protein SH groups. N. Jasinczuk of 50| 75, and 100 ml./kg. of body wt. (BW) (Groups l, 2, and 3) 110577s DNA synthesis in mouse embryonic fibroblast-like over a 30-min. period resulted in extracellular fluid expansions of cells in vitro, stimulated by various factor*. VasU'ev, Yu. M.; 24.4 ab 1.7%, 28.8 * 2.2%, and 33.9 * 5.6%, resp. pCO,was Gel'fand, I- M.; Gershtein, V. I.; Fetisova, E. K. (Mosk. Gos. kept const. Body spaces were measured isotopically, and intra Univ., Moscow, USSR). Dokl. Akai. Nauk SSSR 1969, 187(4), cellular H+ activity by S.S-dimethyl-S^-oxazolidmedione^C. 913-15(Cytol| (Russ). Autoradiography was used to follow the The extracellular bicarbonate concn. decreased only --2.4 ds 0.7 DNA synthesis in single-layer cultures of mouse embryo fibro tneq./l. and --2.5 0.4 meq./l. in Groups f and 2 and --34) = - blast-like cells in lactalbumin hydrolysate and ox serum media in 1.0 in Group 3. The total amt. of extracellular bicarbonate in the presence of thymidine-*// added at various periods of culture creased 4-0.6 sfc 0.2 meq./kg. of BW, 4-0-8 * 0.2 meq,/kg. of age. The index of *K inclusion ws increased by addn. of tes B\V,and +15 0.4 meq./kg. of BW in each group, resp. Con ticular or bacterial hyaluronidases, RNase, and digitonin. The comitantly, the total amt. of extracellular fluid K increased +0.3 rise of JH intake was the result of stimulation of the entry of the 0.0 meq./kg. of BW, +0.5 0-1 meq./kg. of BW, and +0.4 cells into the S-phase of development. G. M. Kosolapoff d= 0-1 meq./kg. of BW, reap., while there was no decrease in 110S78t Effect of pregnancy and oral contraceptive* on glu extracellular K concn. It was suggested chat the extracellular bi cose and insulin metabolism. Tiskinen, Marja R. Duodecim carbonate and K eonens. were defended by shifts of H+ into cells 1969, 85(11), 07(3-83 (Finnish). A review. The effect of both (or bie&rbonete out of cells), in conjunction with a shift of K pregnancy and oral contraceptives is diabetogenous, and they out of cells. Despite the presumed H* (or bicarbonate) flux, may cause deterioration of glucose tolerance in healthy women. there were no net changes of H+ activity. The conceptual The organism tend* to compensate this by enhanced insulin theory of extracellular "dilution acidosis" is valid but of limited secretion, which leads to hyperinsulinism. 43 references. magnitude and littledin. significance when expansion is relatively I. G. Zewi slow. Cellular buffering minimises the extracellular acidosis, H0579u Effect of pyridofal 5'-phoaphate on the strength of and the shift of K out of cells prevents hypokalemia. RCKY skin in vitro. Herleness, Robert D. (Univ. Coll. London, Lon 110584a Effeet of erythropoietin on bone marrow a-amino- don, Engl.). Exptritnlxa I960, 25(10), 1048-9 (Eng). Treat tevutinic acid synthetase and hem* synthetase. Bottomley, ment with 10mA/ pyridoxal 5'-phosphate (I) in vitro reduced the Sylvia S.; Smithee, G. Ann (Veterans Admin. Kosp., Oklahoma strength of rat tail skin progressively with time of incubation. City, Okla.), J. Lob. Clin. Mad. 1969. 74(3), 446-52 (Eng) The tedn. was reversible, with strength returning when the skin An in vitro culture method has been adapted for rabbit bone rings were placed in buffered saline. Pyridnxamine or pyri marrow and has permitted measurement of the effect of erythro doxin* at 10mA/ had no comparable effect. The effect of I was poietin on A-aminoievulintc acid synthetase and heme synthetase reduced in the presence of lysine. Prctrcatrnciit of the skin with activities in'marrow cells. In this system, erythropoietin en N*BH<, followed by washing in saline buffer, largely inhibited hanced A-atninolevulinic acid synthetase activity. This effect the effect of I. Pre-treatment with N'tT;OH ito inhibited the was detectable as early as 6 hra. after contact with erythropoietin. effect of 1. Treatment with alter 1 prevented the re The max. enzyme activity observed was 200' i treater in stim covery of strength cm subsequent transfer tr the saline buffer; ulated than in control cultures. Studies with inhibitors of pro HiNNH* had a similar effect. The effect nf l >>t skin strength is tein synthesis suggested that erythropoietin increased the syn apparently brought about by rupture of a.: .nethine linkages thesis of the enzyme. N'o stimulatory effect by crythropoicti:- formed between an aldehyde and an amino gr. tp. B3JN on heme synthetase activity was observed. RCKY 110580a Diabetogenic effect of i.-asps -aginase. Khan, 1105851 Action of adenosine triphosphate on the depre^se/. Amanulluh; Ariaehi. Mitsun; lliU, Joseph M u-Gta->hau (Wad- spontaneous electrical activity ol the dog cerebral cortex. Brnr Icy Inst, of Mol. Med-, Dallas, Tex.). J. Cliu. F.niiatrinol. Gian; Arrigom, Elena; l-'erruru, A.l hi.iiehcipu, l'. * f Mtlob. I960, 1*9(101. 1373-0 (ting). The effects of Ksrkrrirhia I'harmacol., Univ. Pavia, Pavia, Italy). J. (`Hrm. (. coli i^asparaginaM.- t-*500--f>0,0<K) l.lr. kr.. i.v.), which has been 58(9), l Hil --3 (Eng). The repeated t.uf>prcs*io <-f ilu- vcati1.- uved t<> induce remission of human sictiie tyi'H'hulic and mycUnc- tion and the circulation of the hraiit in uu- (!<>. m<hi. tr> a liiv'ii i cnous Iculccmius.via depletion of an atiiifio acid essential for some pffxtion or a silence of dec. activity of the cortical and *ut neoplastic cells, on rabbit blood and urinary glucose levels were cortical centers. The recovery of respiratory atid circulator.. AP00008661 U39M-1I3MO rOOOCOLOGT [Voi. si | imt 113*65. PAUON, V. N. (Med. Inst., Novosibirsk, USSR.) Q spctskfirhesklkh pnevmoetyakh prl Itlikoa*. [Nonspecific piiwunonla accompanying silicosis.1 OlO TR PROP ZADOL 13(2): 58-60. lie*, --tjoscrvaiions oi it patient* uttering from silicosis and non* *ctflc pneumonia revealed Mut the courae and outeemc of oonspeeUlc nunonla la ouch circa tc highly dependent on the condition ol the nnmrhial tree. In earn of chronic deforming broachilia, the pnea- monia la protracted and reaults In the formation of large loci of fibrous tn the silicotic lung. Chronic deforming bronohltla was chiefly ob served In patient* who had long eeaaod to have contact with mineral durt.--A. M. H. 113*5*. KKADZHIEVA. E. D. (tnat. Did. Hyg. Occup. Dtl., Aead. Med. Set. USSR. Moscow, USSR.) VUyante kapreWclama na pvelevo- dit*r nuyu funktalyu belykh krye. [Effect of caprolactam on the reproductive function fif white rata. | SAJtOT 34(7); 25-21. 19W, [Engl. *um.)-Ti>e eitectot caprolactam waa atudled on the reproductive functions ol albino rule. A dynamic Inhalation pot*onlng of 107 albino female talc after their Impregnation with caprolactam at different concentration* provwd thin substance to affect the conception, the multiplication, the fertility, the course of pregnancy, and the intra uterine development of the fetus In experimental animate. --L. L. 113*70. rZRAEL'SON. Z. I., (SAtor. | Tokalkologly* novykh khtmlchssklkh veshehestv, vnedryasmykh v rexteomyu 1 ahtnauyii promythlennost'. [The toxlcolorv of new chemical* being introduced into the rubber and tire Industrie!. I 235o. ilium. Hedtulna: Moscow. 1968. Pr, 81 kopecks, Trim.: SEE ZH OTD VYP fARMAXOLKB3MX>TER SREDSTVA TOKSIKOL, 1969, NO. 1.54.855. 113971, KUL1NSKAYA, I. L. (Dep. ted. Hyg., Kharkov Inst. Postgrad Med., Kharkov, USSR.) Nekotorye Itmemalya medlatomogo abmena prl ostrem t khronlchsskom otravlentl aerougteradem. [Some change* of acetylenelint metabolism in acute and chronic poisoningwlUTcarbon disulfide, l ~Cio fiAWt" alfflTHka-aj. mm. fengi. siunTj-^n chronic Inlwiealion with C$2 at a eeneentratlan erf 10 mg/m* certain periodical change* were noted tn the cholinergic system of the blood and the acetyleholiae content norepinephrine. After chronic Lntoxlcation for a period of 111/3 mo. a fall of acetylcholine concen tration was noted in the cerebrum, the liver, the kidneys and the spleen. \ certain tendency to decrease the acetylcholinesterase activity in a* spleen was also noted. The norepinephrine content in the cerebrum ad the heart and the epinephrine oontent in the adrenal glands --esented do signincant chuign. Norepinephrine was discovered ike adrenal glands in seme of um rabbu*. After acme polsoabtg ..ch CSJ (12,500 mg/m3) for 2 hr. the acetylcholinesterase activity decreased in the blood and increased In the cerebnan. The concen tration of acetylcholine in the spleen diminished. Acute CSg poisoning brought about a pronounced fall of catecholamine content In the cere brum, the heart and the adrenal gland*. More pronounced shifts of the cholinergic system developed is chronic teitaxicaitoo and la the eympathlco-sdrcnal system la acute poisoning. The acetylcholine syetea Is a sensitive test for detecting functional changes tat the setltm of noxious industrial factors of amall intensity. --C. M. M. 113972. BARSEGYAW, G. B. (Dep. ind. Hyg., Yerevan Med. lost., Yerevan, USSR.) Predel' no dopustlmaya kontsentraUlya 1,3-dlkhlorbutena-2 v vozdukhe prolsvodatveonykh pomeshehenll. [Maxinueii perm isslble concentrattane of 1, l-dtchlorobotene-3 in the air of In dustrial premises,1 ZH~EK8fr KUN MfeD 8(6); 96-72. 1*5*.-- Studies eocducied oa white mice, rets, rabhtt* and hmntM showed that the concentration of this compound is 0.01 mg/1 In & chronic e^erimeat. The recommended penoteeible concentretina for mrkb| estab lishments te 0.001 mg/L--6. T. 113973. DOSXZN, V. A. (Dep. Ryg. Child. Adoteseents, I. M, sechenov 1st Moscow Med. Inst., Moscow, USSR.) O Ghurstrltel nestt organism* podrostkov t yuneshei k pramyshlennym yadam. (SensUtrlt^ of adoiMCrniH and youths to Industrial polwonn. 1 QIG SANIT H(tK W-IM. iD66,~-The evatUbhi llli'ralura 7jh lh'< effect of Industrial tuatc nubfitorrre on growing sdoiiwemts und youths Is reviewed.--J. 5. 113974. ALEKPEROV, I. I., V. 0. KKABENGOF, sodM. !, VtNOKUROVA. (E. M. Efcndi.Zad teat. ted. Hyg., Occup. DU., Baku, USSR.) Puaktslonar now sostoyuite serdechno-sesudlstot slstemy n rabotayushchlkh s uglcvodorodaml nsftl. IFunctlaiial Mate of the carditnraacularsvsUm of persons who work with Detroleiim. hydrocar- boy I Glfl TR^PHOF `ZaDGL 49-50. fterr^gaciil sSdies of 50 petroleum industry workers (20 to 40 yr of ago), who worked under condition* of direet contact with petroleum products, were contrasted with data on 20 l/utHuilonal workers of the asm# age distributed to detect functional ebiSe ia the cardiovascular system under the tefhieaea of rjqneure to such products as styrene, dlvUiyl, ethylbeoeene, etc. "CC changes were basically expressed in email dystrophia alteratloas the myocardium resulting te a slightly Impaired myocardial oca- 'tactile funcUon. OeciQdgnphie study at ths vascular tonus rsnalod a prevalence of cases with e reduced mean arterial pressure, a cna- dltlna typical for perscas frequently sepneei to bydroearbaas. The oaetuogrepate Indsn, which reftaeta Um atfecta of autoaoml* rogulattea. a asymmetric. CaplUaroscopy revsaled apeeUe-atonic functional reactIona with Impaired resistance,--A. M. K. 113975. PENKOVICH, A. A. tlnxt, Ind. llyg. Ocm. Din., Cor'kl, USSR.) K otsenke Ismenentl v matom krugo krovoobrashchentya tt bol* nykh bvlrvyinl zabolcvuilvunt lerikh. JAnsessment of rhwtrna In pulmonary circulation In pnsamcccnloels patirnie. | old tITNiOP " ftABibi, ulaj; su-43. iveb.--Tne character, frequency and ex pressivity of ehlfta caused by chuifes in pulmonary circulation In patients with OCCupttlonal bronchitis and pneumoconiosis were evaluated on the basts el ECO, polycardlography and pulmonary rheography data. Morphological deviations and functional disturbance* tn the apparatus of oxtcrnal respiration are elgnlflcajit In the development of chances In pulmonary circulation In pneumoeenlaels patients. The us* of theaa method* aometimc* rereala even at early stage* of pulmonary la- ufflcleaey, a number of functional shifts in pulmonary etreulatieo, which can be regarded sa the result of aa increase of pulmonary arterial resistance. --J. S. 113975. YAZEN, A. N., and E. L PLOKHOVA. (test. ted. Hyg., Occup. Die., Gorki, USSR.) Dlnsmlka aoderthaniya adrenaUno- podobaykh T*hche*tr v krovl kroifltov prl Uirerdehcikon vaidelstvll parer vinllkhlartda. [Dynamic changes In eolnephrtne-Uke ubstances te rabbit blood following chrome emosure to vtnVl chloride fames,' GI5 TR SHOP ZArot--13(6): -4,TTW8.--Chr5;3c~~ actiai at vinyl chloride hunts units profound fundidial change* in the anterior and, mod particularly, la the posterior nuclei of the hypothalamus. This loads to hyperadrenallsm, resulting la a patho logical tcadc angtoneurodn.--R. b. c. 113877. VOl/FOVflKAYA, R. H,, and I. D. MAXULOVA. (test, ted, Hyg. Occup. Dim, Acad. lied. Scl., USSR, Moscow, USSR.) K vtproau o techenlt angtodlatonleheslUkh eoatoyanil artcrlal'ncl glpsricniet tcfcalcheakat etlologii. [Course cf aaelodyrtcnlc conditions with arterial hypertension of toxic etiology.! oiu TR prof ZABSir lS(U)7 iz-lb. unis. l6B. [Engl. sum.j--5os*rvnllais ware made for aa long as 6 -10 yr or more an 6* motor car drtvere up to the age cf 40 (65 males and 4 females) with a long record of chronic CO poisoning (35 perscas) sad chronic pdaodng with ethylated gasoline and CO (31 persons). Hot* was taken of the intensity cf the sngtodystenle syndrome, each as encephslla, vertigo, dteaetphalle crlaea and BHftlfMtattees of oorontryspasm*. As well as increasing orguie eymptom* whltei were apparent te 15 eaeew, 25 persons develc^ed hypwrtensivw dieesae within the observation period (wioetly at the age of 40-45). Hypertensive disease ordinarily proceeded against the backpraund cf rdsicbtl manilesUttm** cr remote ccnsaquences cf tbe eustahied pctecnlng, betef dlsttepalshed by a specific evolution. Pro nounced vegetative symptoms, diencephalic erlses, syncopes, short lapses of consciousness endocrine disturbances in association with an crganle braia Icstca or affection cf the peripheral nervous system were noted. Hie tewle anslodystcnic syndrome a* well as arterial hypertensHm furthered the development at hypertensive disease. -L. U. L. 113075. SEDOV, A. V., and A. K. MAZIN. (test. Bicphys., Min. Realtk USSR, Moscow, USSR.) K voprosu o ncctnlrovnnil vrednjdck prlmesei to vdykhaemykh gaxovykk smesyakh. [Problem << ttanOardl. sallow of nt^cas dituree la Inhaled gaseous mixtures,| GlGSAHIT 93(2): 9Z-94, 1865.--Dun on gaseous louc substances lODj, CO, NHj) te exhaled air compiled from their permlsslbls cm- centntlcni te tee air cf industrial establlahments show that their --rtim. permissible concentrations do not depend on their ecnesetra- lions ia air srhaled by Inunana,--L, K, R. 113979. VEHOBRSKAYA, Kh. Y*., and N. M. DEhQDBNKO. (Tashkeid Med. teat., Tseh*../.!. US8RJ Meted cpredeleniya pentakkterfmola v vozdukhe prl sel'ekckhosyaltvemykb rabotakh. [Method tor 6*l*rmining actitafhloroohcnol In the air during agTlcuuurarwwlt.i ma BANIT 33(2): 57-08. 1W5.--This mrthod conslsfod oi upiinn liquid mcills (a 0.8'fi mihitim of sodium cArbonste, 0.01 end 0.001 N sohiilms of sodium hyrirratlde, acetano and ethyl alcohol, as well as siltra gell and certain filters. The reaction waa verified with various ageats. Tbs best effect was attained wttb the reaction with plraatdc* (dlmethylamlnoantlpyrtne). Tbe censittvlty cf the method ia 4.5 |if> --la K. 9 113080. MATYSYAX, V. G. O vllyanU paror benzlna na menstniai'nuyu 1 deterodnuyu funktali 1 glndeologlcbeakiiyu sabolevaemaet' rsbetnlts reelnoval promysMennostl. [The effect of gasoline taper* on the menstrual end childbearing functlcKi and the gynecological morbtJilr ol temaie worcers si a avntnettc ruoner lactnrv.i tr azerb nnuuriIBBLEB BlgT Gbi TK MliV IZAUUL 1 g."'M-T)l 1955. Translated from SBP SR OTD YYP TARKAKOL KROCtOTBR SREDSTVA TOKAKDL, 1969, No. 11.54.1199.--ExamlitaUais were mad* cf 3017 female worker* (19-40 yroldj In the synthetic rubber Industry, whose length cf service te tbe industry wma 6-16 yr. Tbure w*r* disturbances of tee menstrual eyet* and menapauetl disorders. A decrease In the evengv number <rf pregnancies, and faunas* in tbs freqracy spadaaecM abeetlais and prematura births. In the amber of Infant* bora dead; as* AP00008662 75521-75930 TOXICOLOGY JV.rf. 52(131] 7554 ^s, *552' KUDRYAVTSEVA. O. F. (Diwrahinek Inst. tnd. Hyg. Occop. %>s.. mcrchtnsk. USSH.l K khenkterisiiko Hrktrckardlnersfieliesklkh amenmtt u bnlriykh khrnniehrskot inlnkslkatstei khlorletym vlnllom. ----(Characteristics of etcetrocardlpgrsnhte chances in pallenii with vinyl riiinrttle prtwng.i GiC Th t'hoFSA'BoL lTnr^4-Tl1'lfllifl.-ega studies of 50 mala and 4) female workers suffering Chronic vinyl chloride oisoning revealed cardiovascular disturbsnces manifested as changes t rhythm, conductance and repolarlxation processes and an Increase - In systolic index. ECG data should be used to determine the eendltlmi of the heart when evaluating the degree ot vinyl chloride poisoning in a patient.--C. ?. 79322. PENKNOVTCH. A. A.. I. S. FACRMAN. and E. V. OLADKOVA. (Inst. Jnd. Hyg. Oeeup. Dis.. Gorki. USSR.) K voprosu ob olsenke i soitoynnlt funktnii vneshnego dykhanlya pri pylevyteh ubolevantyakh bronkholueoehncieo apparaia. IThc evaluation and stale of the external respira tion function in dust-induced disorders of the broncho-pulmonary system.1 GiGTR prof zabol littlt: 7.IQ, 197ft. [ErwL sum.}--The nature and extent of changes In a number of vantUatton figures were dependent on tlie degree of respiratory Incompetence and the form and stage of the coulottc process In the lungs. The results of 1459 examinations were subjected to electronic dala processing. "Hie most specific tndtees Char acterizing the disturbed external respiration function were the maximum pulmonary ventilation, maximum expiration rate and the nrtsrlal Mood O2 saturation. No conclusive differences of ventilation figures in pa tterns with dissimilar forms aad stages of pneumoconiosis could be dis closed. The moat serious disorders of the ventilation function were noted in patients with pneumoconiosis accompanied by clinical manifestations of chronic bronchitis.--C. M. M. 75523. IL'INA, V. A., and T. A. KOCHETKOVA. Gnat. led. Hyg. Occvp. Dis.. Acad. Med. Set. USSR. Moscow. USSR.) Ekeperimental'nyt pnennosklerox pri vdykhanU aerosols! icagnlevalitterykh aplavov. fExDerimental nneumoseleroeia from Inhalation of maeneatum-llthinm alloy acroaoTaTGKrTft PftoP BAlflC HHB: H-jPilW. IBnatJ aum.t-- when inhaled. Mg-Lt alloys hare an irritating action on the muccsa of the upper respiratory tract sod pulmonary tissue and cause a number of changes in the respiratory organs. These include moderate catarrhal tracheitis, bronchitis, interstitial pneumonia, diffuse sclerosis and em physema of the lunge. The extent of ibe pathological process becomes greater as the Li proportion in the alloy increases.-*C. M. M. 7SS34. ZHDAJZESVA. G. 8- O rentgenolecicheskikti Umenenljrakh v legkUcB pri intohsSutntt kaibonit-nlkelein v ostrom t otdatennom penod- 1970, No. 7.54.769.--la 5 men and 2 women <o( whom S were 21-31 yr old aad 1. 92) who had undergone acute poisoning from inhaling nickel carbonyl, observations were mads of the organs of the thorax for 7-12 yr. On the 2nd or 3rd day after the poisoning, a high position of the diaphragm wag noted. The respiratory moverasDts of the diaphragm were not determined or were limited. There was reduced air content of the pulmonary field. Two patients showed intensification and vagueaneas of the lung pieture and of the roots of the lungs and the remainder also showed bilateral feet, Retrograde development of the X-ray changes began on the 7th-12th days. The outcome of the poisoning was favorable in all tbe young people. Dynamic obserraitoaa for 10-12 yr showed nut they were completely recovered. Regression of ths X-ray changes in the older patient was noted until the 30th day. The position and respira tory movements of the diaphragm w* restored in 3 mo. The picture of the hiega and roots became as they originally were in 2 yr after the poisoning. After 7 yr die patient developed pneumosclerosis, cardio sclerosis and stage l respiratory tuidneleney. Nickel earbonyl poison ing is probably not the determining factor in Ute growth of the sclerotic process. Its principal toxic effect Is a disturbance of tbe lung capil laries and arterioles. Acute poisoning in healthy persons does not leave after effects.--S. T. 7J525. OSETROVA, V. I. Soiicyinle sdorov'yt nbochikh tsekha floto* fabrikl Berssnikovakogo kalifnego kombinata po dannym perfodtehaskUch medttstnsktfch osmotrtw. [The health of workers at ttie flotation compound plant of the Beregovskii Potassium Combine, according to data from perlotflc'ohrsical examinations. I TR PERM MED INST 62: 98-K. 1970. Translated from REF ZK OTD YYP FAHMAXOL KHIMJOTER SREDSTVA TOKSDCOL, 1*70, No. 10.94.797.--Of 197 workers examined 70% were women, 50 had worked less than 10 yr and 77 more in the flota tion compound plant of the potassium combine. Hie potentially occupa tionally harmful products there consisted of higher aliphatic amines of fatty acids at concentrations tens of times the maximum allowable with a relative humidity of up to 96% and temperature ts tbe plant of up to 30'C. The paiisnts complained mostly of headache. Of thoee examined. 90T allowed moderate tachycardia, dulling of heart tones, labile blood piwatur* and functional CNS disorders. Tenderness in the right sub til area wta noted tn 19 patients and urobills ni found in the urine oat of these examined. Equipment operators exposed most to amines ai(Owed aa increase In the number of rettcufoeyten and thnmboeytoponla. --8. T. 79926. MOZAN. Cv A. IiAGQUANITT. xnd l. PETTTNATT. fist. Meg, Lav.. Turin. Italy.) Evaluation du risque indult par ic* (oxiqurx indue* triels. [Evaluation of the harmrde -r-ated by industrial toxins.I ARCH MAL PROF MED TRAV SEC S6C StfSi Ul-US. ITO.-Ttu* mean* of evaluating the atmospheric heard created by Industrial toxins that are currently practiced appear fo be unsatisfactory aad open tn criticism. Results oMstned with thorn art Inadmisaiblc. The problem of nnrmtUztng systems of sampling and quantitative determination of toxins ts pres sing and essential. Well-defined norms should b given regarding the speed of air sampling and the point or points where sampling is to be don*. In evaluating ths risk Involved, the phenomenon of toxin condone*, tton upon different surfaces of work areas which boc'ini- urcrmdary sources of pollution must not be forgotten. The only system appearing to ahow this part of (he hexard is that of quantitative determinations in series, even tt times when tho specific risk Is theoretically null, so that charaetertaUe profiles tor each work eyclo can bo obtained. Even In this ease, a standard method should be used to obtain comparable and reprodueibla evaluations. This is the only way to obtain valid param eters of ths hazard created by atmospheric toxins. These parameters art essential for a program of organized and systematic prevention. -3. L. R. 75927. nun, TAMOTSU. (Osaka Unlv. Med. Sch.. Osaka. Jap.) fPneamoconlosls caused be "Sendo" dust amone rush-mat worker*.! KlPonii ACTA ftAMOL TWBrilgW. Ill[us71#70. [In Jap. with Engl, sum.]-- Fancy mat workers are exposed to dense dust of "sendo" (a sort of elay used lo dye rash) in the process of rush-mat manufacturing. In order to confirm ths possibility of tho development of pneumoconiosis due to "sondo", mass survey of workers in domestic rusb-mat workshops was performed by direct chest roentgenography. Pneumoconiottc nodules found in the roentgenograms wars sot uniform tn size. These shadows were mainly composed of punctUorm opacities from 0.9-1.0 mm in di ameter and were distributed evenly throughout the lung fields. Because at tho smallness of individual dust focus, spars# opacities is tbe early stage of pseumoconlouie were difficult to demonstrate tn routine X-ray film. More {meomoconiotic nodules wets usually evidenced la the direct 2-fold mafntfieaflOB radiograms than in conventional films. These find ings were similar to those of some sorts of atypical silicosis and ether nonsilicotic pneumoconioses, especially pneumoconiosis caused by elay Gist tnnautlon. Fibrous thickening of ths pleura due lo pneumoconiosis was not remarkable. KUar shadow* showed no enlargement. Pulmonary emphysema was not so conspicuous. As to the complications in' the lung, casts with active pulmonary tuberculosis were not sen. Of 47 workers with distinct pneumocontoeia, it bad slightly impaired lung function and S had moderate Impairment- FneumencoMotle workers should be removed from areas of high dust exposers to protect Diem from the de velopment of the dlueae*. "Akashl Sendee" which are used In Okayama Prefecture, are compoeed eg about 20% quarts and many kinds of elay minerals. The pneumoconiosis due to "sendo" ts a sort of atypical silico sis caused by the dust which contain* free elites in low percentage and. at the same time, may be Included In clay pneumoconiosis.--C. M. M. 75529. IL'INA. V. A. (Inst. led. Hyg. Oeeup. DU., Acad. Med. Scl. USSR, Moscow, USSR.) Yoproey gigieny truda pri pokiehenil 1 obratxxke msgnievolttlavykh aplavov. [Occupational hygiene in obtaining and traatInc of mteneatom-Uthlum alloys.1 QIP SAWTT 35(101: 24-27. 19T0. [Engl. sum.I--working conditions at the open-pit mining of Mg-Lt alloys are characterised by the almost continuous formation of aerosol and Us discharge Into the air of the working aone. The tsrosol dispersed phase consists mainly of Uthlum oxide, lithium carbonate, lithium chlo ride, lithium fluoride and magnerium oxide. The main sign of the inhaled effect of Mg-Li alloy aerosols 1* the development of an inflammatory pro cess in the respiratory tract and the lung tissues: tracheitis, bronchitis and Interstitial pneumonia resulting la diffused pneumosclerosis and lung emphysema.--C M. M. 73929. BATOLSKA, A., and H. MAJUNOVA. (Inst. Mai. Prof. et. Prat. Trv,, Sofia, Bulg.) Modifications du gluttthioa ehtt Its trsvatilsurs <Tune entreprise metalturgique mlniere. [Glutathione modifications in workers of a mining metallurgical flrm.T arA MAL PROF MED TRAV SECSOC 31(3); 117-122. Ulus. 1970.--A moderate clinical pielure In dicating preliminary occupational poisoning was noted In workers having contact with Pb. As, Cd and Sb. Almost 1/3 eg the workers had lowered Hb. Glutathione [GSH] amounts decreased, GSH Inhibition was parallel to tk* length of time when contact with the substances occurred. The ef fect of glulatlol was favorable with regard to both the clinical picture and laboratory tests. Following administration of glutattol, GSH concen tration. the reduced GSR/total GSH ratio and the amount of serum lietle dehydrogenase Increased.--S. L. R. 75930. ASHBEL', 8.1., R. G. KHIL', I. L. YAKUB, and I. D. VOLKOVA. Cb effdcUvnostl techenlya bol'nykh elUkosom aerotolyemt l elekiroaeroaolyamt ahchetochel. [Hte effeettveneee eg treating silicosis patients with aerosols and riectroaerosols of alkalies. I SB TR NAUCH-1SSLED INST GIG TR PHUFZA^OL GRUZ SSR 12: 149-154. 1970. Translated from REF ZK OTD VYP FAHMAXOL KH1KEOTER SREDSTVA TOKSKOL. 1979, No. 9.94.984.--Of 38 sltteosii patients studied, 19, of whom 12 had ehrealc bronchitis, were treated with a 10% eotutioe eg eodtum bicarbon ate (9 ml by Inhalation at pH 9 for a costs* og 2 Inhalations a dgy for 10 AP00608663 icouxjy [VoU 92(211) 12104 I9i.--in iho devnlnpmrnt of pulmonary eWroAl* In rats studies on change* in the total, estortfled, free cholesterol am) phospholipid* were mode in lunp*. liver and Blood plasma over a period of 200 daya. Total cholesterol, Ita fraction* and phoaphollpM control increased wtth u~- in the luia:s of silicotic animal*. IMcwtwrrUfsllf sudanophllte iala observed In the eytopUam of pulmonary marrsphsitei. ..pea similar to those of hinge were not observed In (leer and blood '-".ami: the increased lipid content of turds during espsrlmrntal pul monary silicosis may be due to the localised degenerative action of silira on macrophages, ; 122299. MUELLER, W., and C.HOLZAPFCL. (Inst. ArteKshyg., Univ., Jena. E. Or.} Ucber die Bc-stehun* zwiachen Delta-Aminolaevwilnsaeurc-Ausacheldttng 1m Ham and Blutbtelaplefel bel ArteHern tntt urKerechItHlictier Sieisxposttfoft, (The relationship between A lA In urine and lead In blood la workers winTtlurei-em load-exposure.I fKT ArCN AftBKffgMED -fljr5Sl-m I(1W ia7r)E.*E^J,)--ta 9 groups of differently exposed lesd-workers the relationship between AlA [gamma-aminolevulinic acid) in urine and Pt> in blood was in vestigated. This relationship W not constart and depends oa the kind and intensity of exposure. It la dlfftsuK to establish an acceptable Umtt valued (he A LA-output taurine. Woman leetn more sensitive to Pbthan men. 122289. LTV5HTT3, O. D, O vocmosJuwstt fxpol' mvanlya mestnykh pcktlnsoderzhanhehlMi plihchcwyVh produMov v prolilalaike saturnisms. (The possibility of using local pectin-containing food products for the prophylaxis of lead poisoning.! TRP?ftM Mku IHST et 134-199. 1^70. Translated from REF ZH OTD VYP FARMAKOL KHtMlOTER SREDSTVA TOKSIXOL, 1929. Mo. 10.54.599.--For * me. lead sestets al 50 mg/kg was ted Intrapaatrlcally to 2-mo.-old male rata. Those of group I were maintained on the usual ration and those of group 3 were given the usual ration * 50% raw carrota, fly the end of the exposure to Pb. the rata of group l had developed the symptoms of chronic Pb poisoning: torpor, disturbances of natrium, lass of hair and a re* ductton In food consumption. During the entire period of observation ail the rats of group 3 remained swJJ, gained weight and had a good appetite, in rata oi group 2 the dally diuresis was 9*11 ml. Oa the 20th-25th day of exposure those of group 1 showed hypouria (3-1 ml). The average weight at the end of the experiment was lM.1 g tor group 1 and 192 g for grenp 2. The kidneys and gastrointestinal bract of the rata of group t excreted 9T.S mg Pb (85% of that taken (ate (he body) * us of group 1, 60 mg (40%), The prophylactic action of local i-containing food products should be studied for use is tbs thera- S^Mtte-propfaytoettc out of workers exposed to Pb.--6. T. 122290. LOSKUTOV, N. F., and N, F. SHUSTVAL'. Umcnenle eiektrokaMiograramy pod vUyanlem tarotononttrllh 1 UokrataaoxittrlM. fChinees in the electrocardiogram under the action of crotonanltrlle and iiocrotononltrlle.l TK laUit'auv "AJsu'lBlr ex. lili-uS), 1199. Translated iron REF ZH OTD VYP FARMAKOL KHIMIOTER SREDSTVA TOKSECOL, 1970, No, 8.54.935.-Hit rats and guinea pigs given a single median lethal dose of erotooonltrile (I) or lsocrotononitrlle (H; used In the production of synthetic rubber), the ECO showed a reduction fit the votfage of the QFS complex, an increase la the num ber of heartbeats to 900/mln, an upward shift from the baseline of segment ST, and the appearance of a high, pointed T wave (tor H days). In subacute espertmsiS* I was given tatragastrlcally to rata at lOOand 50 mf/kg*nd n at 17aad 34 m*/k* for 1.5 me. Guinea pl#t were given an aqueous aohitton Of 2T or 59 mg/kg of I to driftk for 2,9 me. Ths rats showed an Increase in the heart rate, upward displacemet* of the ST segment from the baseline, an Increase in the Tj-j wave to 7-8 mm, a reduction (a ths QRS voltage, aa increase in the pq tderval, and, fa some of the animats, sinoasricular blockade or hwba. at the left branch of the bundle oi HU. Changes of this type were also found In guinea pigs, impairment of the principal (unctions oi the myocardium oceurs: the conductivity, excitability, automatism, and eofCraetlUty, tola la explained by Impairment of (he metabolic processes inthe myocardium caused by the poisoning. There is a bib liography wtth 10 references. From the Ukrainian Institute for the Postgraduate Education oi Doctors, Khar'kov, USSR.--8. T. ^2 22291 aASALAEV, A. V, (Gorki Inst. tod. Hyg. Occup.Dts., OorU, JSSR.i opyt prlmencolya krupnokadrovol ilyuorogralU pri osslsdo4anil akeleta u rabotayushehUdi v kontalde s neprdol*nyml ugtevodorami etilenogo ryada (oHiiAaml) l Ikh khlorprouvodnymt (khkarvinll. trlkhlarstllenl. (Experience with the use of large-irame ohotoltuuroeraphT In examining skeletal bones oi persons occupationally dealing w)lp unaaturatc3 hydrocarbon's cl the ethylene series lotennsi ana thsir chirlnv~drhmiTvea <vinyl chloride. lrlchtorcthylene),| Gld TH PROF w \L'14{U): M-37. mo. |cngu eura.)--Tne#e examinations re.d a definite reconstruction In the hones; osteosclerosis, oeteow sis, osteolysis and cystic changes. Traumatizationof ths limbs -ad prevalence to the clinical poisoning picture of these symptoms 12135 )Nnv. 1.1911) TOXICC mnmeslrd lesions of higher vegetative center*. particular|y of the hy pothalamic rri'bin. These chance* mav be oi a wurodvelrnphic nature. LArfie-lrame rAoto*taurofrayhy may he recommended fnr rnerAuenolngical examinations pi the skeleton in occupsiioitst croups Wth potential none imtlwlftRy.-C. M. M. 122292. AMIROV, R. O., 1.1. ALEKPEROV, and V. D. FTVOVAROV. K sanilarso-ciRlcsIchesbol tearahterlstlko uatovli truda rahotayunhchlkh prl pnhichcnil kntaplna (IKIKhP-2). fA saeitsry-bvgicmtc charsetcrltatint. f iuiMrt.1-- ...Jttu-. -f-------------- 1 I 1 ' - 1 ,,J'- ' ' FARMAKOL KHIMIOTER SREDSTVA TOKS1KOL,'l970, Ko. LS4.I319. 122293. BRAKMINOVA, I. T.f and G. V. SAMSOKOV. SravnlteToaya otsenka vllyanlya na orcanlam nltrldov percldiodnyidi metailov 1 neme- IComoaratlve study oi the eifeet o/transiHanal mwi mctamcnUrwcs on the bektv.^ gigtr PR^f fJIRrtl. IlHi). JB.an I970[rccd. 1971).-In experiments with white rats nitrides of nonmetals Including BN, SI3N4, and BNC had weakly pronounced (taro* sente activity and caused pnesmocoaiotlc chanacs. Mrtal-llk* nitrides including TIN, ZrN and NbN In addition eaqsed dystrophy of the liver and spttheltum of the renal cccvoioled tubulsa. The rccommeikled maximum permissible dose for nitrides oi non-metals and NbN is 10 mg/m tor AIN U 4 mg/m3 tar zrN u 5 mg/m3. Ths toxic f. reels oi nitridss corresponded to Utelr electron structure. Their "jolfkrtcat activity decreased with the increased pwsfbUUy of forming SZp^-wlectron conflgurallons durtog Interaction with N of noa-tnetals and semi-ntetaia and alto dS-eonllguratlons with Uw formation of nbrldea of (ransittonai metals.--N. L. G. 122294. KARIMOV, A. M. FrotesslonaVnye zatoofsvaniya kotoi u IthtojiiMrobuv vysvannye yadotetmUcataml, 1 meroprlyatiya po lMt ---V- uermamis ana eczema occurred In 30 persons out of 32 In direct contact With DDT and hexa- chlorane. The disturbance* were of a seasonal natws. Of 933 cotton growetw, the akin was sensitive to the pboapheorganlc insecticide* methylmercaptophoe, octamethyl and butyphoa u> 42%. The time of development of Sensitization to different chemicals varied aiM depended on body response, concentration of the chemical, work conditions and the nature of contact.--N. L. O. AHi98^* A`8." V- A* D'VACHENKO, V.I. IVANOV, and A. P. TAUN. Opyt medUamentonaol protitaldUel porsahenU nervnai sietesny pel tatokelksUil ognsstoiklm masions -Iwk>r-3". fExoerlence In medical oreyewtioo of tahtfr to the nervoos aystemln Soisonliuf with I9"0Trecd. 1871),--person* woeidrg without gu masks in the remain* ot a breakdown at an electric power station were intoxicated by the tomes of "lvtol-3*, a compound based on irlxyleny[phosphate, vitamin Bp vttamlA VKi phytia w nwvoui flytttme'-fcf. L. Q* ia preVtnfclnf damiM to tho 122299. ELBUTSKAVA, R. V. Sostoyanie plodovltestl u ehivotaykh prl knrcnlcheskoTu vozdslstvll parov mstadisopropUbenzols. FCoitdltlOB of fecundity to_anlmals subjected to chronic effect* of metadlisooro- ' wiosazene tomes.! fcli tR pRflF ?.*guV. Ul\i\. ii-IA" 1971J.--'White rats (45) wtth strictly periodic oestrsl cycles were ex posed to the tomes of aetadUsoprepylbenasM In eonesiftrattons of 0.2 reg/i and 1 mg/1 5 hr dally 5 times a wk for S mo. The course of the Sexual cycle was distorted with characteristic prolonged How. The capacity (or concession wo* substantially decreased. The average mmter of offspring decreased as wall as thsir weight. Geaadotroplc effects of the compound arc indicated. Women's work In (he presence of this compound should te regulated.--N. L. G. 123297, SUKHAREVSKAYA, T. M., L, S. SAVEL'EVA, and A. I. VOROTNIKOVA. (Novosib. Sanlt. Inst,, Novosib., USSR.) Opyt prlmenonly* t -amlnokapeonovol Klsloty prl Miranlcheskoi totokslkMsll bensolom 1 ego gottiotogaml. [Experience >- <-anninocapronlc acid -wlthKa hornolpgues.l 6lCTR~y5gy ZABOL 14(12j? If-Ai. lS75frei. ia71|.- -Amtooeaprontc acid, vuamuis B j and Big, glncose and F* preparations wees used to treat 12 patients with chronic poisoning by benzene and Us bomologuea with symptoms of hemorrhagic diathesis. A hemostatic effect was obtained n 12- days In patients with mild symptoms of hemophilia and In 2 -3 day days to those with pronounced manifestation* oi dlsthesls. in addition to the hemoatalie effect, capillary permeability tad the fibrinolytic acftlvtty of the blood decreased and blood coagulation time speeded up. The number of mature platelet* Increased after treatment and the num ber of eld thrombocyte forms increased.--N. l. G. 132299. MAN*HQ, N. N. (IBM. Hyg. Toxical. FeMle. Polym. Plait. U?5.`l Dte*w*v*t* (tedsl'ao dopustlmol koMsentratsll boch 0r* Ihtton of ate maximum permissible ccnceatrMloa of Foxatou ia the sir of a TOhisifB,nS!a mTR tXBUL 14(H), ^MDus. lB7^z's5!liwi".!ih;' AP00008664 470S (Apr. 19. 1*71] TOXICOLOGY 48319-4632S cleaniref, hydraulic wash-out) and hygienic (axil-dual respirators, protective pasira) meinares were recommended. Introduction of such measures at a number of electric power a Iat tons helped to drastically reduce the concentration of fuel aid cinder dust In the atmosphere of vnrk premia** and to do awav wtlh acute and chronic nrcupailnnal dU" ea.--C. M. M. . i9. PAVIOVA.L. P. (M. M. ErcMI-*adr Inal. Ind. Hvg. Oceup. Dia,, Sumgait, USSR.) Material? k oboanovanlvu prodeTno dopuxtimol kontaentmthli dtikoprTrilbenisftlnv v vocdtdche prnlzvodMvrumkh pomenhehmll. IMaterlala for pstahlUhlag ihc maximum prmiteiHbic cone miration of tlllscprnpyl benzenes in the air of Industrial premlara.! GIG TR I*h6f ZAKl"Util: <T-4Ii. lilua. |!Wo. |KnpL Mmt.J--The comparative toaleltv of m- and pDirn |dUcpi*apvtoenieno*| waa atudlcd at lethal, toxic and threshold concentration* and dotages. D1PB are virtually devoid of cumiilatlvi-capabittty. Chronic action of thetr fumes at a concentration of 1 nig i produced CN8 Inhibition, disturbance! of Ihc anti* toxic-synthetic and prolein-lorralnp function of th liver and sharply pronounced primary leukocytosis with subsequent tendency toward* Irukrpmla. Concentration of 0.2 mg l wss the threshold value In a chronic test. Dim (0.0Sm>; |) wan the recommended maximum pcrtuisstolr concentration for Ihc atmosphere around work places. <8320. SIMPSON, G. ft. (Div. Oceup. Health, Lideombe,' N. & W., Aurt.) Exposure to dual lathe crAton-garneUltuc Industry. MED 3 At'ST 51-lllJ): 1101*1162. 1970.--Six cotton-garnettlng plants were examined and eotton4hwt concentration* of 8 to 21 ms'es m were recorded in the Inqilam atmospheric breathing-zone. Tesla of lung function of the operators showed an over Hill average fall of forced expiratory volume (FEVi) of 0.12 1. In more duity areaa, an average fall In FEV of 0.28 l waa recorded. At dost cowentra* Hone in the vicinity of 8 mg- cu m, little change in FEV^ occurred. As the air-home duet was noi entirely raw cotton, a figure of s mg cu m of cotton dual In air is suggested an a tentative thresh old value for this type of work. The use of exhaust ventilation u reectnmended to reduce the concentration of cotton dual in air. --C. M. M. 48321. MEL'NIKOVA, M. M., L 8. IVANOVA, and B. A. YAMPOL'* 5KAYA. (Cent. fnat. Postgrad. M*L, Moscow, USSR.) Kklinlke khroniclteskol intoksikatsii okia*yB ugleroda. (CUnlcal picture in chronic carbon monoxide poisoning.I GIG TR PROF ZABOL 14(9): 23-26. 1970. (EngL sunl.|--fleaulls of u examination of 188 wo- > engaged In servicing taxi-fleet garages of tiie city of Mj . whose occupational activities involved their being exposed to ihe effect of elevated concentrations of CO exhaust gas are pre vented. A high incidence of functional disorders implicating the CMS, appearing In conjunction with vegetative-vascular dysfuaetioit, hyper tensive reactions and ECG signs of focal and diffuse myocardial changes, was noted; A high carboxy Kb Itvsl in these persons con firm *d the etiological role played by CO in the development of these changes.-^. M. M. 46322. BLAGODAT1N, V. M., I. A. GOLOVA, N. K. BLAGODATKINA, E. p. RUMYANTSEVA, L. A. GORYACHEVA, N. K. ALIEVA, and 8. A. GRONSBERG. (tnst. lod. Hyg. Ocean. Dis.. Gorki, USSR.) Voprosy glgteny trudt I prcfpatologii v proisvodetve organlcheakoga stefcla, [Issues of Industrial hyglenr and occupational pathology In rhe manufacture oi organic glass,] (ilG *rH phOP aBql 14(B): 11-14. 1970. (EngLauni.j--Fourfactories manufacturing organic glass (pglymethyiacryiace) destined for different purposes were renewed. A feature common lo all of them was air pollution in work promisee with methylmethacrylate (MMA) ftunts. Its eoneentra* uau varied within a wide range. MMA produced an adverse elfeet on the hcakh of tht workers, manifesting Itself In the development >4 chronic occupational poisoning.-^. M. M. 4G323, Gf.iKSllTKlN, M. D. Sar-tu-wo imotwnwifitt arK-riai'nngo tiinuuj u raix-UtuHlieliikh a ma|n*iornvmvml noftvaml. I Li;n.^li.T,.r" H-rvio.- ri |,it --rt iranirox 'if Ihr artorvnl tnUH In pormswi wnrWJnW*' iIf, In'*1 --nuIIur t>i~li'tili-urnT] Tft A^Kflll NAUOfl-fS&l.ED INDTuIG Tn'NtO;7AlioL--Ur?!CT4. 1969. From; REF OTD VYP FARMAKOL KRIMIOTEfl SREDCTVA TOKSTXOL, 1970. No. 1.54.1915. 48324. KHLEBNIKOVA, M. I., E. V. GLADKOVA. L. T. KURENKO, A. V. PSHENITSYN, and B. M. SHAUN. (last. Ind. Hyg.Gccup. Di., Gorki, USSR.) Voprosy glgteny truda I sostoyaroe sdorov'ya rabochlkh v proixvodstve o-toluldina. 1 Problems of industrial hvsiene and health siaiua of workers encaged in the oroCuctlctl of iHoluiaineT MG TR PHOFZAroE'TyiTi'T-fa. 197BV lEngll' aum.|--Wfurk candlllnns and health statua of workers engaged lit the production of o-toludin* were studied. The air of work premises increased concentration* of o4ohildin* and o-n4oluea*. Som kere demonstrated dyanric manifcetatlana, Initial patho* l'4pe. .iterations eg Ihe urinsry bladder mueoea and, In Individual tnstaneaa, bladder pepiUceaa*. These changes and dieordera were c< an occupational nature, speetal experimental investigations are needed lo determine ihc cxfcnl of the hazard caused bv each toluldlne Isomer In rrrmrd in Ihe developtnenl of urlnsrv bladder tumors. -c.m. m. 46325. AGARKOV, P. T.. and S.V. AGARKOVA. 'A. M. ftnrkll Med. Inst., Donetsk, USSR.) O temperature wdvkhacmouo vozdnkha i konditslcmirovushchct funfosil dykhairtVy:* apparala Tdorovvkh l bol'iwkh parvmnknntnanm shakbterov. (Temperature ni exhaled air and conditioning function nf the respiratory apparatus in healthy and Bncumeceeitoaln-affectm mincrsT) 010 Tit PROF 7.MtOE---11(21; Jl-34. 1970. (t'rtcl. *um'.|--Thc expired >ir temperature was registered In 864 practically healthy and pneumoconinnls^kf(feted miners by an elcetrothermomHer provided with a highly sensttiv* and tov^nntla thermistor aenaor. This van paralleled by the nmsuremeni of vital capacity of the lungs. The data obtained (armed a baais for detarmlidng reserve conditioning potentialities of the respiratory system. The expired air temperaturo in practically healthy miners is approximately the nn) as In normal subjects not engaged in mining, averaging 24.37*0.03*0. In pneumoconiosis and Its progressiva development, Ihe temperature of expired air gooe down. This is accompanied by a distinct decrease of reserve conditioning possibilities of the respiratory system. The intensity with which the conditioning function declines augments as the pro* ctu of pneumoconiosis progresses la its development.-^. M. M. 46328. MARIN, A, M., and A. O, NAVAKATIKYAN. (Inst. Ind. Hyg. Ocetq>. Dla., Donetsk, USSR.) KUniko-fisiologieheskeye kharakteriatlka pylevogo bronkhl*a a polzeranykh raboehtkh ugol'nykh shaJcbt. fCUnlcal-physlologics; characteristics of dust-Induced bronchitis In underground ccal miners.! QIO TR PROF ZhboL 14(2): 2s-Ji. ievo. [EngL sum.|--Patients [70j with chronic duetinduced bronchitis of long standing were reported. Transformation Into (17.1$) and suspected pneumoconiosis <8.67 of eases) were noted after a lapse of 2-8 yr. In patients with bronchttis odtlbltlng no signs of pulmonary flbrosts, non<11 seated misers and practically healthy Individuals with no service record in an atraoapherle environ* meat of high dustiness the vital capacity of the lungs (VCL), resid ual air `(RA), total king capacity (TLC), maximum volumetric expiration rate (MVER) and maxlram volumetric inspiration race (MVTR) were determined. When measured, these indices ware contrasted againet Ihe due values. A distinct rise of RA and an appre ciably fall of the VLC, MVER and MVIR vers recorded In patient* suffering Irent chronic bronchitis. Increased RA figures were registered also In non-diseaaed miners with long service-record, proving early development of manireetatlons typical ot emphyaama and deranged pulmonary ventilation.-^. M. M. 46327. KHENOKH, E. A. Gigfeaieheskaya ctsenka uslovii truda rabotmyuahchUdi prt mokrykh pretseasakh mekhanlcheskof obrabotk! isdelll tz atdels I khrysUlya. (Hygienic evaluation of the worictuK eoadltlons In wet machining processes of glass aid crystal ware.) CHS TR PROP'ZXBOL "nU)~45^E."197ff. --Wet procasaes of machining glass, crystal and quarts ware (grind!og, pollahlng and (seating) are accompanied by consider able release of quartXMSontalning dust Into the air of the work rooms. Grinding and polishing ore done mostly by hand and require appre ciable physical exertion an the part of the workers. The consider able relesee of dust makes It neeeaaary to equip the grinding and polishing equipment with ettiaut fens. The Kcupallons of grinders and polishers should be included among these that are deleterious. These workers must undergo mandatory preliminary and periodic medical examinations.--J. S. 46328 SMIRNOVA, N. A., and N. P. CRANDC (Gorki Inst. Ind. Hyg, Oceup. Die., Gorki, USSR.) Ob otdalennykh posledstviyakh profsealonaTnykh ostrykh ocravlenu nekotoryml uglevo* dorodami 1 Ikh proixvodnymL (Loog-lenn side effects of scute occupational poisoning by certain hydrocarbons and their arrivalI venj GIO TR pKSfzADOL I4(SI; 5O-51. 1870T^Ta xludy id Ihr nldr rffc'CiH of occupations! polnnnlng in 35 person* (olrflrtN, 0; benzyl, 5; luHhytcnc ehlwrlrir. 3; vinyl rlilorldr, 2; IrlchWiriw'lhyli-ne, H; rtldilornWImnn, 8; lexrachlorot-lhane, 1; chlorobenzene, 3; poiyehlorobutane, 1) is rnpurlnl. In alt cates rosldoal effeeta In the form of functional disturbance* uf the CNS, symptoms of sympathetic-sensory polyneuritis or organic dis order of the brain were observed. The frequency and degree of axpresalce of these side effects depend upon the severity of Intoxi cation suffered, previous CNS diseases and the Um* of return to work after nvurot roplc poisoning.--C.P. 48329. ENTERLINE, PHILIP B. (Grad. Seh. Pub. Health, Univ., Pltteburgh, Pa., USA.) Validating thresholds using Industrial records, J OCCUP MEff 12(2): ~ 34-37. Illus.' 1970.--Industry ha* orne fairly aeeesslble records which might bs useful In establishing Threshold limit Values for esrtals substances. These art empteym sit and exposure records on retired populations and records on mortality In these populations. Data available from these records nest all the requirements for good epidemiological invrsil pattens. Thar* might even be sons advantage in poolit* retirement AP00008665 OLOttY (Vot. 55(0)) 3994 uivn in vivo selectively Inhibits (or 10*60 min * nuctsoplaemtc type of "NA polymerase la rat liver but th synthesis In vivo of all species of tvar RHA romalna blockad for **veml hours.--J. T. L. 3 0ROOK6. ROBERT B. Jr. and JAMES A. VICK. (Dap. Rata* aeok. -altar Red Army Inst. Re*.. Walter Read Army Med. Cent.. Washington. D. C. 20013. USA.) Toxicological studies ot bee venom In mice, doge and monKCTi. AM 3 llili): 250-351. 1913.--Venom ot Apia meiHicra we Injected. either l.v. or a.c., into experimental animals. lA', injections eavaed a precipitous deereaae la blood presaura within 3$ aee In bath doc* and monkey#. It atao decreased heart rate and depressed rvsptrallox. Dosages o( 5 rax/kg In dog*, or 2.S mg/kg in monkeys, i.v., caused death from cardiovascular (allure. Similar response* were noted aftr a.c. injections, but median lethal donors ware higher: 300 mg/kg i data and 50 mtg/kg In monkeya. The former ia equivaterd to about 3000 baa stings. Smaller dosages were fatal to mice. Other physiological reactions are noted. --J. d. Hitchcock, 34171,. ULANOVA. I. P.. A. t. KHALEPOandA. 1. EITIN0CN. (Inst, Ind. Hyg. Oecup. Die.. Acad. Med. Set. USSR, Moscow. USSR.) lasledovante kumulysUvnykh svolstvichlnilrhesklkh eoedlnenti ne porogovom urovne kak osnova proenoairovasiya khroalcheakikk InlokaikatSIl. (Study into cumulative pronemes ot chemical campounds at the thresh old level as a basis lor woenosttcattan at chronic potsonlngs.i cao TR PROF zABOL 1613); 3409. JSlZ.jEngl. summ.J--Cumulative properties of chemical compounds at the threshold of their acute action were analysed. Cumulation coefficients war* deduced from the integral teat changes and with reference to tbe summary-threshold value fea turing the (imcUona) state of the nervous aystem. These coefficients proved to bt much inferior to eoaifieieata of cumulation determined with reference to ttta animal lethality llgsrts. The cumulation coef ficient* at the threshold level supported a well-marked cumulative ca pacity of bensene. carbon tetrachloride, wi-smlnobeftioLrinuorWe and morpholine. This was supported by the data derived from chrome ex periments, considering QClq and ft#mens, also by the availaUa clinical and hygienic observations. The estimated coefficient of correlation between the area* of chronic actum and coefficient# of cumulation for the substances under study at the threshold level waa 0.99. This makes it possible to forecast possible development! of ehronlo poisoning. The investigation time was cut down to a maximum of 1 mo.-*J. P. K. t 773. KOSUUYA. N. S- L. E. GEDYWN, P. R. vAKHOTEKtl and O. 9. ARKHIPOVA. (Cant. Xtea. Inst. Tobere., Mteklealtb USSR. Moscow, USSR.) Imchenl* pronltaaemostt gietogamansheiktUi bar'erov prl nxHchnykh rahtmakh gtpsrbaxicheAol perlmentally, 70 gidaea pigs were aub)aeted to 100% Og in tharapaattn (1-3.3 up to 3 ala) and lode (4-S ate) regimes of byperbarte oxygenatioa for 1-4 hr. Histology sad radioactive Indication (with atrsptomyel#4" In 30,000 U/kg and with 14.3 pCi activity ia 1 ml) war* used to assess various permeability terete In the histo-bsmattc barriers of the luage, liver, kidneye, apises, myocardium and brain. Toxic regimes were accompanied by a wivereal Increase of vascular wails permeabil ity, conditioned by atmetcnl lesions of the Msto-hsmatlc barriers. With therapeutic regimes, vascular permeability ruse only la the spleen. Uvr and kidneys, la., la.tea er^aa with as increasing functional ac tivity u&dtr hyperbaric oxygenation, and was not accompanied by hlatoteoctuaal cknogec of the mate etementa of tee hlata-hamatic barrier. --J. u s. 34714. CCNBER, D. W., p. C. OLOFF8 and Y. S. 5ZSTO. (PaatoU Gent., Dcp. Bid. Sci., Simon Eraser Oahv Burnaby 3,8. C,, Can.] GLC sesaration te heottchlnr ewiiu nwipuordane. a- end y-chlordsne. SOLE EWlftCW CO/utafaflflSI; TOta-ll. llW.'w*CLCIg " 1 liquid ehrcamlogragfiy] eohuuas ware used to identify and quantitate orginochlortde piattcida residues. Such aa 3% OV 1, 3% SB 30 plue 6% OF 1, or 3% OT 1 plus 4% 09 310. They did net satisfactorily sep arate the oompmmda ofa-Chlordaiw. y-chtordaos aaychlordafte and hspttchioreiatlde. Gas chromatograph Micro Tdc MT 330 with Nl*gJ electron capture detector method was employed lo this experiment. --J. P. K. 34175. WALTSCHEWA, W. (Foraehungelnet. ArbeUaechuta, BsrufAr., Blvd. Pvtko Napster 54, Sofia, Bui*.), M. StATEWA aad l, WCHAiLOW. Hodenvertsederungen bet wetsaen Rattea dureb chroalacha Verabnlehimg vcw NiefcelsuUat. (Testicular changes due to lotmterm administration of MAel sulfate le ralt.i 'ffxt1 patHOL UBMAT' 6(3/4): 1 is-igo. uiua. 1B7Z. (Engl, eumm.j--Following tang-term 'sulfate lotmdcatiDn fet rata, aavere tesuos in the germ calls V Aarly in speralogeoeRS could be demoaatrated. Very few ebamt- - aA , observed la the livers and kidneys. Nickel aultete bad a eetec^ jealous effect on testieuiar pareachraa.--A. B. M. 34TIS. BASALAEV, A. V., A. U. VAZW sad A. a. KOCHETKOV. V. H. Xinw cent. Med. fast.. Gertl, USSR.) K yntegaMaa Ismseerut, voatekaynahteiflth prl dUtel'aom vaadelacvtt rtnikhtorl*. tPaUioweneaia 35511Mar. IS. iB1J| TOXICOS/ of chsnnra rtrvelonlnn due to long-term --rv^wrr in me effect of1 vinnyyl-- ehloridr.l fgr. TR t'ROf ZADOL~ I6lfl: Ulus. 1*72'. (T.ni;l. iurmuTp-nabblia and rata which inhaled vinylehlortur fumes tn1 a ` dk Imal permissible concentration {0,034.04 mq/l), und-rwcni a 8- long nil-round examlnallnn by physiolA<>eal. bkoeh-mical. histo>mm nrphologleal and raentgenodansttornctrlc methods. Ctuaips in the. 1h._u_v^ electric activity of the hypothalamus, hyperadremimenua and function al troubles nf the cardiovascular system were Uinck.sed. De\-clopfnent of resorpUsa manifestations in the bond (issue war brought out mor phologically and osteoporosis--roantgtnoleqlcaUy. An interenee 11 draw* as to a key Importance of hypothalamic dysfunction in thi patho genesis of the disease, this dysfunction giving rise to iMurobumoral and aeurovaieuter troubles leading to the development at dystrophic pro cesses In the bone tissue,--p. L. W. 34717. BAZIN, SUZANNE and ALBERT DELAUNAY. tCSRS. Inst. Pasteur, Sent. Pathol. Exp., 93-Gnrehea, Fr.) Action emreee [nr do* aubstences lathyrogenaa aur la collagens de granulomes. (AcUtmexerted by lathvrogenlc aubataneaa on the coUaccn ot granulomarTl C R KEBD SEANCES ACAD SCT SERDSCI NAT (PARTSf ZU(4|: 6i8-f]20. 1973,--Polyvinyl apongee were Implanted a.c, in Wlaur rata. Ten days later, penicillamine waa given orally in a dose of 30 mg/100 g of weight. In another group, g-mercaptoethyUratne waa given tn a dors of 0.5 mg/100 g, l.p. Granulomatous tissue surrounding the sponges and nor mal Rein were extracted to give neutral and sold soluble collagen, col lagen labile at M* and insoluble oollagen. Both drugs gave comparable results. They did not modify the total collagen of normal Ain, but decreaaed the collagen of granulomatous tissue slightly. Neutral soluble collagen was Increased and Insoluble collagen decreased In both types Of tissue. The labile collagen increased in the granuloma and dimin ished la the Ain. The acid soluble eoBagan increased 300% is lbs graaulonta and 30% in the Ain. InUraoteevter bonds la collagen which are sensitive to lathyrogens are comparable m gyaauloau and tn nor mal Atn.--M. D. S. 34714. KUDZ1NA, C. D. Ahd D. I. COLOVAN'. (A. N. Maraeev* Kiev Rea. IntL Gen. Public Hyg.r Kiev, USSR.) 8ra*nitel'uya oteenka gvr- Mtsidov gruppy khlorbenzolnol klsloty uoUamo*tnksikologicbeALa mstedom i matodom kul'tur klatok. (Comparative sanitary-toxicolog ical and eell-cuiture evaluation of the chtorbentolc acid herSiclde group.! VBACK faELO 1. 135-138. luue. 1973. [Engl, summ.l--Pate oTenroale Mnitery-toxlcotoglcal axpsrtmeM on rabbits and a study of the effect of ehlorbentotc herbicide group indicate that the results by these 3 methods concerning tbs toxicity of the herbicides coincided. The use ot ciU-wdtins method tor rapid hmeaie evaluation of the tox icity ed some herbicides is premising aod (utter iaresUgsUons eg this method are (uatUied.--P, L. W, 34719. COOPER, PH1UP and STEPHEN B. TOURS. (Dep. Surg.T Bronx Veterans Admin. Hasp., Bronx, N, Y, 10463, USA.) Relationship between smoking history and ewaplications immediately following sur gery for duodenal ulcer. "Vr gWAf J mEd 39(3): 381-39371972.-- No evidence is given of aa saaoctetlBn between smoking and complica tions, nor betwaon smAing and operative mortality, nor between smAIng and days to discharge. The most obvious trend is probably predict able, namely, patients over SQ have more compUcaUoas and a higher mortality cate than those under 50. ta spite ot the vetatively Urge number of patients included in this report the smoking habits, the age distribution, sod disease rate In the population studied are quite likely to differ from tee general population. Some lnconsistenets* wen found betweea smoking histories taken oa the same yatisot at different times. This may have resulted from the quality and accuracy of the informattm given to the examiner by the patient. Few, if say, generalities should be drawn from this research. Results apply only to immediate postoperative findings Ad do sot apply to tong-nags effects of statedng upon the patioot after surgery for duodenal ulcer disease.--E. M. D. 34730. USTEA, F. M. and L. A. MOTROTA. (Ftc. Postgrad. Med.. Rigs Med, !nst,, Riga, USSR.) O klinlcheskikhosobonaostyakh alkogol'- nykk polinsvritov. [Clinical traits of alcoholic polvrieimlts.l ZH NEV- ROPATOL PSKHIATR1M 5 S KOnSAKWA 1973. {Engl, summ.) --His traits of eleoiwUe polyneurttie in 27 patients were studied. Pour main clinical variants ot the disease were dlstiagteehed. Tbe most characteristic and frequent form of alcoholic polyneuritis waa the syndrome of sluggish paralysis in tbe parts of the lower extrtmi* ties. A mors common and mixed form of polyneurttie. seneoriel asd aiactic forme, were rarely observed.--J. P. K. 34731. 8TBOER, ROBERT B. (Coop. Ext. Sepr., N. M. State Uuiv., Las Cruces, N. M., USA.) Nstlvs plants Ptesonous tc humans. 3 RANGE MANAGE 35(1): 71-1271B72.--A sumiBary U ivea"<rf the tewle prlscipies, the plants, toxic parts, symptoms and some general comments for several commonly tadc plants in thw U8A.--D. T. S. 34732. PARIS, RENE-RAYMOND and ANNE-MARIE TESS1ER. (FaC. FDarm., Lab. Matter* Med., 4-ave. Observetotrv, 7S-Parls 5, Fr.) Presence ds stestaoess da groups dee Cucatbltecises chsa diverse* Eupboruacees tcottqusa afrltttets, aotemmest ches Maprounn stem* branssea Paa et K. Rota. fPrvsence of stewtaness of the group of cucwWaelMi in various Airicaa tonic hswrtuctet eaaeeuily ln 15-* AP00008666 1167 (Jan. IS. 1073| TOXICOLOGY 11446-11456 ENVIRONMENTAL AND INDUSTRIAL TOXICOLOGY also: Pabllr Health Ocnjpilkxul Health, R.idlallna Health f 11445. JONES, R,D. (Fhartn. Dlv,, I.C. i.Ltd., AUerleyPaik, Marelrsfteld, Cheshire, EngL, UK.), B. T. COMKMS and A. A. CFRNOC. Blood land rtnd carboxThaomoeloWn Levels la London taxi drivers,, LANCET (NORTH AM ED) 2(7T72):362-J03.1972.--Amonf StTLondoa taxi <lrtvra the mean blood-lead 'was 26,7 jig/100 ml. There was no difference sf statistical significance in the blood-levels of smoker* and tion-smokers, nor between the blood-lead* of night-shift and day-shift drivers. Cirboxyhemoglobin levels erf smokers were significantly higher than those of neo^mokere, and the levels were etgniftcantly higher in day driver* then In night driver*. f 11446. MC NTIRE, MATILDA 6. and CAROL R.AN0L2. (Dep. Pedtttr., Untv.Nebr. Coll. Med,, OmalU, Nebr. 6(106, USA.) Air lead: Petition to lead in blood of Mack echeol eMldren deficient la ghtcoaV-q-phoaphate dehydrogenase. SCIENCE (WASH) 177(4046): 520-523. Ulus. 1972.--Black children (44) at 2 slcmentary school* w)thkn-0.7 ml of a battery plant had significantly higher (P <0,01) concentration* of Pb in their bloods (34,1 * 9.7, pg/IOOmm, mean * 3D) than 122 students (26J * 7,1) at 7 schools 1-3 ml distant; 9 noT later then was a comparable difference between red cell Pb value* (54.1 * 18.S vs. 37,4 * 12.9). Among the blacks, those deficient le glueoee-6-phoiph*t*'3efcfdrf*njB* had a higher (P< .009) CDucentralien of Pb in the blood after correction for enemla {32,9 *9.7) than the nondeficient (25,7 *8.8), and a higher concentration & the red cells (47.3 -14.7 as compared to 154 * 154 P< 401); the ensyme effect waa independent erf geographic location. t 11447. MARKOWITZ, SAUL $,, CHARLES J, MC DONAU3 (Roger Williams Gen. Ho*p,, 625 Chalkstone Ave., Providence, R. 1.02909, USAJ. WILLIAM TETHERE and MARVIN S. KERZNER. Occupational acroosteolvsis. ARCH DERMATOL 106(2): 219-222. nlu.lBIl-- Oecupattenal acreoeteolyel* la an wniwai disease in which attested persons have acierodermoid-Uke leslooa of the skin, conditions similar to Raynaud's phenomenon, and asymptomatic, bony defeet* of the extremities. Evidently, the disease Is confined solely to workers la the plastic* industry, specifically (hose engaged in the profeetioa of polyvinyl chloride from vinyl chloride. The incidence of the disease is miaows bat Is thought to occur in 3% or less of workers. 11446. WILDISH, D, J, (Pish.Res. Board, Can. Biol. Sta., St. to aquatic fans than polyoxyethylene estate, Polyoxyethylene (4) lauryl ether u 9 times more toxic than polyoxyethylene (14) mo* lanrate at the ILL [incipient lethal levels] totoLiio laiar and 14,009 times mere toxic m the M-hr LC50 [lethal concernralca median] to Gammaraa Oceanian Sereeetri-le. t 11449. HOFMANN, A. (Inst. Toxtkol., E.Merck Darmstadt, D-6100 Darmstadt 2, West OerJ aad M. NEUFELDBR. TtoraxperlraenteUa UotiieuehuBgea car gewerbetoxiteloglaclten Beurteltung ven 2-chtoraethyllsocyanst. (An investigation of 2-chloroethvl isocyanate la laboratory animals witn regard to me imolicatitas ae a ooteottal onHAasATh55ai iSf!M TUiiMflc^iSi irr-Ti.-gifmr1197*. [Engl, aumm.j--The acute oral toxielty of l-chtoroeftyl Isocyan ate (CAfC) is 396 rag/kg for nul* rats and 630 mg/kg (or female mlcw. The extremely small quantity cl 5 ul CAIC euaeed severe irrltattm in the eyes cf rabbits, particularly of the ccnfuncttva. The (Ida wss also affected considerably. Inhalation experiments, involving a new ver*too erf a proven method for generating air-vapor mixtures, pro duced so evtdeoce d systemic toxicity, but let CAfC bo classified as an Irritant vapor to the respiratory tract. The USjgfx rate 1* 64 ppm after exposure for 6 hr, white rats in 2 experiments involving INay exposures to vapor eeaeeatntiCM <f 3,1 and 34 ppm, respec tively, survived. After a fortaighfa withdrawal following each expertmeat, histological examinations rtwwed trachsobrcnehltt* and bronchopneuocnia but oo pathological change tn any other organ examined* A threshold limit vahi* (TLV) erf 042 ppm was suggssted. t 11450. JOSE, FRANCISCO R. (Dep, Thyslot.Ryg.Mute., UBlv. PhilippManila, Philipp.) A study of lead hawi in imh lead procam toe induetetee, ACTA MED PHILIPP 9(2): 48-94, 1972.--TWO bat tery plants, 2 paint eetebUehmenta and I company manufacturing lead oxide were atodted to erataate the extent and intensity of pb toward. Method* ol erabutton used mrt levala erf Pb In air, and urinary Fb, 1 tippled red cell count and clinical manifestations suggestive f Pb intoxication among employees in the eetabllahmeAtt. Levels erf Pb a air, Pb concentration tn the urine and stippled red cell count mie .igher te the lend oxide end battery plants than in those engaged in pale* marafaetoriag. Cfinical manifestations suggestive ed Pb poisoning wen also mon fnqosat. Good correlation was ebsertsrf betwuqa tie laboratory fladteg* ud the chafe*! oralfestetions ladlenttv* erf mild Pb potecuing. 1 11451. GOODWIN, JOHN W. (Day, Zlmmcrmjuw, Irc,, Lone Star Dlv,, Teaarkaaa, Tex. 76901, USA.) Twenty years httadllnr TNT in a shell loading plant. AM IND HYG ASSOC J Sill). tl-4t.tl)u*. 1973,--In the handling cf TNT within (he munitions industry, human exposure In thin substance cannot be completely avoided. The goal of a Preventive Medical Program is to prevent such exposures Irnm causing any permanent damage to workers. Exhaust renli Litton with wet scrubbers, use of protective clothing, mandatory praetkre of good personal hygiene, and careful medical supervision have combined to accomplish this goal. The vse of the Thymol Turbidity Test in such a program was a valuable Indicator of Uvcr ms ifunction. t 11452. JCRAMER, C. O. (Dow Chem. Co., Midland, Mich. 46640, U3AJ ana J, E.MUTCHLER. The correlation of clinical and envtronmental measurement* for workers oxposod to vinyl chloride. AM IND HYO ASSOC J "53(1): 10-30. Ului. 1971.--A mcthnd is described for the statistical consolidation and correlation of environmental measurements and clinical findings using as an example a group of healthy main workers exposed routinely to vinyl chloride for periods tip to 35 yr. Retrospective la nature the stady reveals several statis tically significant effects from chronic exposures to vinyl chloride and traces of vtnylidene chloride. t 11453. BL-DAKHAKHNY, ABDEL-ARlZ and YASSIN M. EL-SADDC. (Dep, Occup. Health, High last. Public Health, Alexandria tlftiv,, Alexandria, UAR.) Lead tn hair among exposed workers. AM IND HYO ASSOC J 33(1): 31-34. Ulus, in!/--In a sfcidy performed on 67 Pb exposed workers, a correlation was found b*twain level* of Pb In hair and the blocheaUai and clinical (indinge. A Pb level In hair erf more than 30 ug/g could be considered as a sign of excessive Pb level. t 11454. LAUWERY8, R,, R, DBLBROECK and M, D. VENS. (Unite Toxicol. Pathol. Travail, Catholic Unit. Louvain, Ave. Chapelle-lux Champs 4,1200 Brussels, Belg.) Automated analysis of delte-amteo- laevullalc acid In urine. CLIN OffiTiKTX-BRJJfT^^TfTnSST 1973,--Tha nilttleo of an Internal standard of 5 -amteotovullnle acid to the vine allows the colorimetric determination of this metabolite without the need of * preliminary chromatographic separation. The apaed sad accuracy make the procedure Nitebte for the periodic control of workers exposed to Pb. ' 11499. MKSIC, J. and 8. HUKDVIC. (test. FarmakoL. Tlksi)uL,, Med. Fak,, Sarajevo, TbgoslJ Vzroct nastajanfa kxcnlcaog opetnikttv- nogbronhltlsa u radfikn one metetorgijo. (Causes of chronic obstnietiv* hrnnckltie in fevmna mntnllni*** worker*.I ARH Ifir. RADA futCUr SlU)t 4-J2. Skua, iftffyread. 1475). fengi. eumra.)--An analysis was performed on 400 people wrung and tiring under differ ent conditions ot exposure to tor pollution. Respiratory symptoms were questioned and the ventilation function of the lungs, O* and COj content of arterial blood, pretence ot allergic phenomena, cate on smoking habits and the oensequenees in pulmonary aad cardiovascular systems were toadied. The 6 metal tons that constituted the dost were Investigated, Experiments were also performed on frog* (Rang rtdl- *-- "-J.--J. F.L. ------------- 11466. CONCHARVK, E. I,, A. A. POLYANSKII, I, L JHVAKO and A. A. CEDCDf. (Dep. Public Hyg,, Kiev Med. teat., Kiev, USSR.) Fascvy* lsnenentyn funktall ahcUtovtdnol timtesy toys pri Mkronloheskom voadeteMl dlbromantina. (Phase aWernttcna of fiirroto function la rats exposed to the chronic activity ol dibramanUiinj GIQ SANIT 36(10): 1Q3-I0ii HJu" lJ7i[r*c<f. IJH).--rt'e"*uncttaial condition of ttja thyroid vu studied in rate with 1-131 after drinldng HjO with dlbromanthin eonceotratiens close to tiioee found in swimming pools disinfected with this chemical, te tea 1st mo,, there was some excitation of thyroid function, fa a 4 mo. experiment, this Utter etfeet was reptocad at later periods by pronounced Inhibition. Harmful effects on humans are not certafa^-K. L. G. 11467. FROLOVA, A. D. fOp the methods of studying btotranefomaties ot Poisons in organisree.] PRAC LEK 3113/31: 91-99. 1972. I Ctech. and Engl, surers^--To* mechanism of the toxic ellect ol udaatrial poiaoos was discussed. The Uck of methods tor identifica tion of chemical eubeteaeee and products cl their transformation tn biologic material, made flieae studlea rather (ttfflcult. temple methodic approach afterdtnig indirect assessment of the fate of toratm aubsteacaa tn fa* organism had not yet tost Its Importance, The effectiveness erf proposed simple procedures for studies erf the fate erf poisons was demonstrated with 3 representatives from the series of tluorated compounds.--i, A. L. 11496, FSEROVA-BERGEROYA, VERA (Reprints: Ven PiseromBergeron Thomas, 146 9.S. 2Slh Road, Miami, Fla, 33129, USA.) aad J, LUDVK CETTL. Blehlrtcky model pro vutnbavam meteboUsmus n TykKCvanl hraaeaa u ctovekn. (Electric model for the abeorottnn meWboUsn andI ycraticn irf bsatene a maaJ PRAC LEK 24(1/3): tWO, nku ,1972. (mss. and Engl. nan,plhi vakiee tor the reeltteace and eapnclttea in the electrical analogue were derived by analogy to ffcystoLogteal factors for e 70 kUe man (blood flow in tieeea. AP00008667 INTEOfVEJSTAHY dYJTEM J'4 :T.rl* trcEniau* for me*unne skin rnnrfudKuv. MFD BIOL ENo 10 4': 3fl-5*J. Illu*. 1972,--A simple jwrtaMn apparatus l* described for monitoring the conductivity of fti skin. After the physiological rationale of the technique Is appreciated, a moans of assessing nonspecific responses to various stimuli will he provided. --J. j. B. 8433. CRAMER. H. J. (HautklJn. Med. Aksd., Klemnt-G<4twaMSt. 34. DDR-SOl Erfurt, East Ger.1 Unteraachunsen rum Narhwels gebundener brw. masklerter Lipid* in der Oermate-Hlstologle. IStudies on the detection of bound or masked ilplda In derwatohfoi.oftgrn dehmatol monatssChR 157<T3T:"SSFfigSV TTTua. 191l| reed. 1972). fEngl. summ.)--Four methods were used to de tect masked lipids Including: loiter staining time. Meter temper ature. Rerenbaems'a C-mrthod, and the Sedan-Burnt Bercnbeum technique*. These methods were poor In 75 blopetta of various dermatosis. The Importance and problems of paraffin sections, Sudan black as an indicator tad control extmotion* were pretested. --A. V. 9. 8432. SCUTT, R. W. B. (R. Navy Hoep., HasUr, Gosport, Rants., Enfcl., UK.) The ehemlcal removal of tattoos. BR J PLASTSURG 2512'b 1394m. nius. 1973.--a modification of a welMrted cbstakal technique for removing tattoos It presented as a safe, simple and affective ott-pntleat procedure requiring virtually no aftsr-car*. A sdutlow of tannic add, glycerine and distilled R0 <2 :l jl) la swabbed over the ares and tattooed Into Uw skin. A alive/nttnte stick Is rubbed over the ana.--J, L, 9, PATHOLOGY Bee also: Allergy Immunology {ImmuzochereIstry), especially ImmonopaUMlagy {Tissue Immunology! Medical and Clinical Microbiology {includes Veterinary) Neoplasms and NeapIssue Agents Parasitology (Includes Seto- ud Endeparasites) Pathology, General and Mlscellaneoua - biHimnatteu SJtd Inflammstory Diseases Public Health Epidemiology Toxicology 7 9434. THCffNIEH, H. (Unlv. Raidkiln., D 74 Tuebingen, West, er.) Experimental!* Eatzeendung dsr {taut bel Tier end Maseeh. Ensrimental Inflsmmattoa of the akin In animals and raarul BIT inSHi PHARRAkGL ThER lUHAOL B(I)g lW^BTnfis. 1871 freed. 1971). [Engl. summ.1--Severn! models of Inflammation are described without detailed oats on experimettal performance. --J. J. 8. t 9439. SZALAY, OLENN C. {South. Calif. Perm. Mad. Group, 1050 West Facuis Coeat Highw., Harbor CHy, Calif. 94719, USA.) and E. DAVID WEINSTEIN. Questionable Bloom avndrona In a Negro Klrt. AM J DB CHILD "124(3); 54S-54a, fUus. IMS.--The Bloom synBrome, a genodannatosls, 1# described hero for the flret time in s Negro; the patient dM not manifest telanglectatte erythema, one of the cardinal feateree of the syndrom*. A new subgroup of the Intrauterine growth retardation syndroms was not postulated. Her conditio* satisfied the other 2 major criteria of the Bloom syn drome uid multiple minor criteria, including perental consanguinity and chromosomal changes. t 8435. METERSCN, LAWRENCE B. {Ii-vii* Med, Prof. Village, not htocArthur Bled., Irving, Tex. 75081, USA.) and GERHARD C. MEIER. Cutaneous lesions InacmoatoolTSla. ARCH DERMATOL 108(2)- 334-321. am*. 187J.- -A pet hint with Idiopathic sc roostsOlysis had unique papular skin teutons. Evidently, almlltr lesions wore previously metuioned only In conneetton with aersasteolyais sees la vinyl chloride processing, and e detailed description of these was not noted in the literature. t 1437. GIROUX. JEAN-MARIO 'Hotel-Dleu Montreal, 3349 ' Saint Urbala, Montreal 131, Qua., Con.) and ANDRE BARBEAU. EtythTokemiodcmla with ataxia. ARCH DERMATOL 108(2)? . 183-lift. Dlna. l97Z.--An apparently new naaroeutaneoua syndrome wen oboemed In 3S mamba re from over 5 generedons in a family of French Canadian ethnic background. The syndrome te otereekerlsed by the appearance soon after birth, of papulosquamous erythematsws plequea which have mild variations la Intensity and whtoh teed to sob* aide during the cummer meiUhs. In meet eat**, the eutaneoe* maaifrntatlons almost disappear near the age of 35, sometime* to reaptaar alter the age of 40. At that time, a alowly progressive man- -leal eyndrome, with decreased tendon reflexoa, nystagmus, urtluli, ttd sever* gait ataxia, become* the predomlnem feature. - rhe syndrome Is inherited In an autosetna! domlnaid fajhtoa, t 8438. DITTO, JOUHt, SEFPO LDfDT, REDCXITURTO and LB DANIEL*EN. (Dap, Dermatol., Untv, Capeak,, Blmdamsve) 9, UN Copenhagen. I>n,l Lactate dehvrtmcenaa* in panudnxenfh'oti* elasticum: Totnl articItT'anj iaivnxvme 7intHH<itinh 'in th akin. .rBTrarrcimttiwraefiviy and lanrnavme distribution of lactate dehvdrngrnase (LDII) vrerr an. aavrd In the akin of lallrniit wilh nsruitoxanthnma riantleum 't'XF.l. Tim values obtained from lesions nf 9 pallenls with typical PXE were compared to those obtained frnm 8 ago-matehed control*. In the akin, all LDH Isoenzymes, With the exception of LDII,, could he detected, I.DHj being the predominant one. The Isornrvrne pattern In PXE was characterised by decreased LDII acrivky In iaoenxvme fraction LDHg and Increased In friction LDII?, as crimpsred (a the eontrola. In agreement with these ctonqrs, the relative proportion of polypeptide M, the sub-unit predominant in cathndto isoenzymes, was decreased In PXE. Total LDH activity was not chanced In this disorder. f 8439. MAPGOLB, MARVIN H. (Cep. Dermatol., Unlv, Wla. Med. Cent., 1300 University A*., Madison, Wla., 53788, USA.), GARRETT A. COOPER and 9TURE A. M. JOHNSON. Keratosis Itohennide* chronica. ARCH DERMATOL 1*5(1)? 7d'4-T43. fllua. 1972.--A pailcrt ftad a unique 15 yr history of a progr*s8lv*. recalcitrant, papulosquamous eruption and persistent hoarsen**!. Histologically, lho erupt toe showed parakeratosis, orthohyporkeratosti, atrophy of the makplglilan layer and a llehanold histiocytic and lymphocytic dermal infiltrate. This cutaneous eruption, previously unreported, should be designated "keratosis lichenoides chronica." t 9444. FBHER, LOUB >3. (Dhr. Dsrmatol., Serlppe Clin., Rea. round., 478 Prospect St., La Jolla, Calif. 93037, USA.) and HOWARD L MABACH. The effect of occlusive and temlpermotble dressing* cn the mitotic activity of normal and wounded human epidermis."Tig J DEPMATOL 86(9); 5W-B(>.1972.--Occlusion Increased the rate of closure of superficial wcunda and inhibited cell division both of psoriatic lesions and stripped skin. Four dressings: an occlusive, adhesive tape; the adhecive backing from this tape: > Its* occlusive adhesive tape: and an exclusive, non-adhesive film, ware used to cover regions of normal and stripped skin for periods from 2-9 days. A slight Inert*!* to mitotic rat* and epidermal thickness of normal skin resulted. A decreased mitotic ret* and increased thleknese of stripped skin, under all dressings was foend. The less occlusive films may be useful in Moderating the healing of wounds without adding the risk of Infection ssen under compute occlusion. t 9441. STEKBTROM, STBN (Dep. Plastic Surf., Unlv. Hosp., Umia, gwed.l, FRANK BBtGMAN and SVEN BERGMAN, wound _ heello* with ordinary ndbeaive imoei A clinical and eaaerlmemai sjjjv? StfSBPJ PLA8T RECWsTB gTlUfl' iff): ^Ollua.' I5WT--1The good clinical experience of treating human wounds with ordinary adteshrotspe Is presented. These experiences are compared with the results of experiments m animals concerned with the healing time of wounds and histological and bacteriological conditions. T 9442. LEWTN. KLAUS (Dep. Pathol. Stanford Unlv. Sch. Med., Stardord, Calif. 94305, UBA.), SHARON DB WIT and RICHARD A. FERRINGTON. Pathology of the fingernail In psoriasis! A cllnlco. csthologlcai study. BR J DERMATOL SMO): 555-583. fllus. 1972. -.-Seven cases wre etudlnd. Psoriasis of the finger nail produces a raetaplasue change of the nail matrix and mil bed to ekln-llke epi thelium, following which the hlatologteal changes are essentially similar to those seen to the skin. The resulting lesion* of the finger nail are of 2 types? nail plate abnormalities, the aatur* of which de pend* upon the sft* and the duration at disease to the matrix: and nail bed abnormalities, consisting of onycholysis, subungual keratosis and splinter hemorrhages. 8443. GWtEZDZWSKL SENON and BOHDAN 6ZTSZYMAR. (Stud. Med. Seiw. buprov., Bydgostee, Pol.) Dwa preypedkl mastocytoeto cetta eollfaria fnaevitorml*)u dstecl. (Two eases of maataertosin cutis solltaria (naevlformla) In Children.) PED1ATR POL 4B(lh mum. mua. Ulllmed. 187S}.-Tk(> 3 Sported eases of thla disease are charsct#rl*cd by a rarely recorded localization. Derler's aymptom Is Implicated tnthe clinical diagnosis of some skin rashes of the birthmark type. The rashes were found on the face and neck. A positive Darter's symptom is more indieatlvo than histological examination. Microscopic analyses may prove mis leading owing to the trauma.during sectioning and possible Inac curacies during th* making of elides resulting indlslntegratleeof the oietschrom# gmnetotlcnu to meat cells. --(Courtesy NSF PL 13-480). PHYSIOLOGY AND BtOCHEMSTRY t 3444. OHKAWARA, AKIRA, KENNETH M. HALPRDi a201 Northwest 9th., Miami, FU^ USA.) end VICTOR LEVINE. Th* enavm** of streccen metaboHstn in the varloua cell fractions from the normal mirnaa mMtrmto. J &VHt bfcmiATOL wire 187- liV. niua. oormal human epidermis, glycogen syntte- tass to sedira*nt*d wXh glyeogea as to tte giyeegen ynttetase D phoepMaeo Which oowvsrt* the D to th* 1 form. Glycogen eyntfaetise AP0000I 3602. LQNO-TEHM SCP08UHB TO YTJTfL CgLOBISB jtlha, u. lugi, C.-E, (1972) Sklsrodermieartige D*u.tv*rlndar- ungen, Raynaud-Syndrom uni Akrooateolyeen bei Arbeitem der PVC- hsrstellenden Inductcie. Dt. used. Vachr. 21> "'922. Exposure to about 300 pp vinyl chloride say have an adverse effect on liver function (BXS3A Bull. 1972, 11, 492), and under certain cirovunstanoes industrial exposure to vinyl chloride aay be followed by softening of -the bonss of the extremities, by neurological end oirdil atory disturbances and by liver enlargement. The paper cited above describes seven patients who reported symptoms of illness after working for 1-3*3 y* on polymerization, processes for PVC production. Their symptoms included hypersensitivity to cold, psraesthssia, deafness, blanching of the fingers, pressure-pain in the finger-tips and lose of grip. Later, swelling of the terminal finger joints and changes la the nails developed. In single cases, failure of vision, abdominal pain or giddiness was reported. Five patients developed Baynaud's syndrome, five a drumstick-type thiekening of the finger-tips and prominent hour-glesa defamation of the nails, and five had swollen fingers with nodular infiltration of the finger wabs, the back of the and the region distal to the elbow. Sanded osteolysis of the terminal phalanges of the fingers appeared in five of the seven. Clinioal teats showed indications of liver dysfunction in two of the patients and obstructive respiratory disease In one. Three patients showed skin changes characteristic of the early inflammatory stags of s progressive solsxodesma. So far, the pathogenesis of this array of effeota remains obscure, AP00008669 8*1*1 Chgwfcvrf Aottrmc* Vol. 79,1*79 Bof-t '.0A in intracellular media in all persona under study.' lie Ci level in getaa. GtisravaaM, K. A. G. (Aktlebolag Baaoe Vvs'/i^u^, alaed plasma decreased with ifi> The K level m intracellular Sw.d. 352,817 (Cl. B Old), 15 Jan 1978, AppL W73/7; m May media increased with ace, that in interce iular liqs. wa* practi 1971: A pp- Thr app. cootistr of *rtxberptiroirs'.Mnt" i. cally const. These findings agree with the literature data u( the limestone suspcittibii at its bottom,* tube reaching nr:.. _.!> effect of occupational stresses as in this case high atm. air temp., above the absorbent surface and through which fur .*<*., par. noise, vibrations and industrial dust on electrolyte metabolism. from the bottom carrying drops of absorbent t#... top th* F.. Stromberg -23162( Importance of poloaitunr210 determination In the ttriae of workers ia uranium mines. Panov, D.; I'airork. D.; Novak, L.; Djordjevic, V. (Inst. Labor Med. Radiol. Prot., Belgrade, Yugoslavia). .furrier. Mtd, 1072, 2301), 0-1.0 (Bulg). A study of 299 workm in the uranium mines of Yugo* cnli imn, a circulation line from the top of the cjiuim to its L- .v -j--. Ijt returning sepd. absorbent to tfcebotito. and a mill locate! n. the circulation lute for grinding the rwdmulaicd absorbent. . I. G. 2ewi 23191k Determination of micreamouats ef some substances in air-. Geeiev. G A.: SotoVov, E. E.; Filippova,*S. A. slavia who were exposed to Rn as well as rats living under the U.S.3.R. 386.409- Appl. 29 Sep 1970. same conditions showed that it is not possible to del. the con From Otkrytiya, Isobret., Prom. QSinoti), I*rowa*aZnabi. 107.*, tamination by Rn end its degradation products by testing the 50(7), 84. The analyzed sample'of air was panned chrucgfe c-< urine tor MPo. No correlation exists between the cones, of ,wPo entrainment separator filled n ib a fine-grained absorbent (durir: - in the urine and the conen. of Rn in the work environment. wilingj to cooc. the iute'ar.. bein'/ <uul>'zed io'k'atd- J. J. PoM healing and blowing tne emv1. ubsc*?. i' wi;:, i ku cirri"..- 23183g Hand tremor induced by industrial exposure to la* the gas analyzer ferfurthsrde'r.. indmordinc. Toino, t>... * organic mercury. Wood, Ronald w.; Weiss,-Ann B.; Weiss, accuracy of anal., air was partly absorb! together eith acul- Bernard (Sch. Med. Dent., Univ. Rochester, Rochester, N. Y.). substances. During subsequent beating, air be:. - A as x -um -i A reh. Environ. Health 1076,28(5), 249-52 {Eng}. Hand tremor for supplying the substation into the gas analyzer. in 2 subjects with industrial Hg exposure decreased after cessation 23192j Teat tube for carbon dioxide measurement. Auc-- of exposure and the subjects became asymptomatic after 3.5 gesellKhaft Gmi.b.H. Ger. Often. 2,151,693 (Cl- G OH., 19 Apr months. The extent of hand tremor was evaluated by requiring 1973, Appl. P 21 81 693.8,13 Oct 1971*, 8 pp. The trees-uria* the subjects to maintain a force of 10-40 g with the forefinger on a range ofCO* test tubes, useful for automotive egham>t ea >es, wu finger trough attached to a strain gauge. Performance improved extended by an AJtO*-supported KOH prelayer. Tire layer v:; after the subjects were removed from Hg exposure. made by drying in vacuo at 100 a mixt. contg. Al-.cv, Raymond Zehnpfennig mm). 100kand KOH f*8v KOH in72 mlHjO) ]Tm:a..d ' itien- 23l84h Ssuitsry-hygienic appraisal of working conditions ing with IitO 1 mi. A iO mm Sung bed of this ms j.. )(.. during the welding of metal covered with protective coat*. Prot- vol. % COiat ten gas vol*. of 0.11. senko.G.A.; Danilov, V. I.; Timchenko, A. N.; Nenartovich, 23193k Oxidation catalyzer for exhaust gases '-VLtj-iab;- A. V.; Trubilko, v. I.; Savdhenkov, V. A. (Khark'. Xauchno- Seito; Uchida, Sutnio; Kori, Yotfaizo; Kav.st-^r.;. Issled. Inst. Gig. Tr. Froftabol., Kharkov, USSR). Automata Okamoto, Osamu (Hitachi Makuseru'Co., Ltd.- tCoiu. Svarka 1973, 26(2), 65-8 (Russ). Air samples were taken using 73 27,988 (Cl. 13(9)011), 13 Apr 1973. Appl. 71 fc: 4, 12 Av, a specially equipped welder's helmet and analyzed for dust, 1971; 3 pp. A perovskiie-tvpe AaBi-aMOt >A, elk. eartp. phenol, CO, HP, acrolein, phtbalic anhydride, NO,, Mn, and metals; B. rare cai ih metals; Me. Fe group metals; x,0.(il-0.9l SiO|. Tests were run with and without general ventilation. oxidn; catalyzer for exhaust gases from a car or chem. .i :* Weldings were both head-operated and automatic on a 10*mm prepd. by heating the acetate mixt. at 900* for 6 hr, p^wdcrii,;., coated steel p!at,e. Gas-cutting was also examd. The coatings mixing with NH4HCO1. pressing in a pipe-form, and heating at were a giypthallc (I) a' phenol-formaldehyde (II) and a poly 1200* fot'1 hr to obtain a stater of 86% poroeif. .x work> we'.* ethylene (ul), in 35 sun thickness. The results are compered even at 1000* for long periods and is aotpoisonki by PL>,,3 and \ with the max. permissible conens. 1 is the least and HI the most compds. .:Icdami. objectionable Q, with ZnO, is toxic. Olaf Thomsen 23194m Adsorbent of poUonoua gases. Ins... j'.wst- 23185J Hygienic certification of the building projects de- ,, aipmahn.. VKonkUa.ii 7733 7277,9O3BO9 /(ra-i.. 1la3</c9)vGt1i1i^^), I1S3 Apr ? TI.. velopment, ana reconstruction of plants producing orgsaocbloriae 4 0,973, 13 Aug 1971; 4 pp- N and S oxides in extuust saj> products.' Levina, M. M.i Filatova, V. S.; Belaya, E. G.; absorbed on a spintl-type oxide contg. one or tr. re rr.-, Ashirova, S. A.; Tikhomirov, Yu. P.; Levin/A. L.: Mostin- metals, e.(. Cn, Ti, V, Cr, Mn, and Fa groups an . u;ii (kayt, R. Z.; Ruanetsldi, B. B. (Inst. Gic. Tr. Profsabol., alk. earth metals on active carbon and dtsorbe Gorki. USSR). Cig. Sanit. 1973, (4), 88-7 (Russ). Certifica soln. * - t .-'i' tion of. building plans, jdevelopment, and reconstructions of 23195n Device for recycling exhaust gas 1,: a *; Slants raanufg. PVC along with a favorable solution of sanitary- - Cuason, Charles (Pan American Chemical Co. Inc. S. Afzima ygienic problems appear to depart from hygienic requisites. 72 01,665 (Q. C 103), 25 Oct 1972, Fr. Appl 71 12.130, 09 At- Poor ventilation in plants manufg. vinyl chloride, polychloro* 1971; 6pp. The exhaust gasas of a hut engine jt* rtc> ded prene, end trichloroethylene it poor and does not conform to . 'reduce the amt. of NOa in the final effluent. The exhaust bz.-*' ttds. Architectural modifications should be employed to effect are recycled beiow the discharge for return u> the intake; lit hygienic stds. John Howe Scott gases are passed Into a catalytic purifin. unit where the vo . re 23185k Studies e! combustion with a sludge burner. Wits- cycle of exhaust gases is varied with supply of Uis\ to the vs^ae. enberger, J. (Cer.). Watter, Luft Betr. 1973,17(1), 24-0 (Cer). Henry H. Gir-.^rj A 40 to 1600 kg/hr sludge burner burns combustible industrial 23166p Smoke abatement ia the oesulfurixauoa of p.j iron. waste sludges with a rain, of soot or CO. The sludge is atomized Braetsch, Peter; Domalski, Willi: Behrens, Manfred '.:oahl- in the nozzle by a stream of compressed air or steam and elec, werke Peine^alzgitter A.-G.) Ger. Offea. 2,150,90S (Cl. C fired. The burner fires into a suitably insulated muffle furnace 21c), 26 Apr 1973, Appl. P 21 50 965.9-24, 13 Oct IS" . ( pp- chamber from which combustion products may be removed for The development of smoke iu the desulfurixatio- .7 -rtm wv anal. Using a 100 kg/hr model burner, old crankcase oil and lac reduced by.adding aoda-cemcnt-fluorite mixta c-'-L..g. quer solvent wastes were burned with a min. of soot or CO. Less flammable materials can be totally burned if other flammable Bases ai* used in place of compresed air or steam. . J.li.Leflia Na metasiticate for fixation of the volatile Na.v1 ; raw' bv riecompn. o( the soda. A mini, contg. <oda 03, portUnd cement -> and fluorite 11% was suitable for thr desulfurizatiou >vf Fe at 1350-409*. * 23l97q Removal of sulfur oxides from chimney gas. Ta 23187m Aircraft Emissions: Impact on Air Quality and naka, lakushi; llattori, Hiroshi; Matsumoto, Sei.ii (Sakai Feasibility of Control (Office of Air Programs APTD-0757). Chemical industry Co., Ltd.) Japan. Kokai 73 26.089 .CL United States Environmental Protection Agency, Office of Air 13(9)F2), 07 Apr 1973, Appl. 71 52,.27,13 Jul IWTl. ^ ,.p. SO, Programs (USEPA, Off. Air Programs: Research Triangle Park, X.C.). 1972,60pp. 23188a Air Pollution Aspects of Emission Sources: Pulp and Paper Industry. A Bibliography with Abstracts (United States in chimney gas are 94% removed by passing, at 1U mi (min fix hr,' through ivlumn of 800 g of 4-6 snn diem- prlTv-'v eo>-.;e SrCOs, which are prepd. by mixing SrCCb with br-r xite >diatomaotoua tank and pitch, molding, and ignii.;,,' Environmental Protection Agsncy Publicsties No. AP-121). The paUau are rsteneratsd by trutiag with 20% Ns.<. < -< United States Environmental Protection Agency, Air Pollution Tedinical Information Center (UfiKI'A, Air 1`oHut- Tech, la- form. Cent.; Researt-h Triangle Park. N.C.), 1873, 108 pp. 23l89p Air Pollution Treariatiana; A llbkemahy WUk Abstracts. Vol. 3 Waited 8tiui Xavirearaeatu Protection fee 3 hr. CnCOa and BeCOi are unsuitable. K. 1% . 83188c Potaseluin auUate roeevery In the purity* Mliuc dioxldo-cemalnlni waste tases. Vun * SehibUla. Eduardifitrewe, Wolfgang ir'-lr. 1 ,. Ger. Ofleo. 2.14T.4IT iCI. It mrf-. , y. T \ Agency Publication No. AF-130). United States Environmental 427.1, S3 Sep 1971; " fl* 7nv wu u-e<* J'rotri tion Agency, Information Barshe* tkvision (USBPA, Air Pollul. Tech. Infer. Cant: kmeetch 'Triangle Park, N.C.). 1973,250pp. contg. N, CO, and CO, with . SO* by washing with a Roll-KCl *>!n, ublai. . . 1. -a,u..w 1 from a diaphragm elecirolyi: plain. The Kjfit.1, iiinnnl i-. _ ^_ __ eeemtetvurrent waslung pro-v^. is oxidized by ou w O-enr , air at 60-100*/3-2-i atm and kCl is added to the oxidised win. 23i90g Absorber for purifying sulfur dioxide-containing flue lower tbe soly. of K*SO. The K*SO is filtered off and the fii- a- AP00008670 Pag* 149 59--Air Pollution, Ind, Hyn. VoL 79, 1973 130497 ' 130484k Product* of tha thermal breakdown of expanded occur, possibly dnring tlie next century, nnd will melt polar ice polystyrene. lnvanyuk.K.C.; Kobzar, A. I. (USSR). LilttHtHt caps, decreasing the tdbedo of the Earth's surface, slowing the ProizvrJ. 1972, (10), 41-5 (Kus*). I-lxpaiKlcri polystyrene of deep circulation nf tlte Adrnus nnd the cycling of nutrient*, in the different types was pyrolyzctl at 1500* and tlie product* were oceans, increasing sea level, flooding coastal plains, and increas analysed by a very sensitive chromuiog. ihoOuhI. 'The gaseous ing weathering proce**** nnd changing both animal nnd plant products contain lit, CO, CIU Cjll, Ciiti, Cll. Ct1!, CIl. ecosystems to the detriment of human ixipuliitioas. J. Pick 139491k Atmospheric reloases from Tarapur. Abraham, I*.; 139485m Nitrogen oxldei, nuclear weapon testing, Concorde, Pattnaik, !>.; I';tnirncsv:ir;m. 8. (Hhnhlui At. Res. Cent., and stratospheric oxone. Goldsmith, IV, Tuck. A. K.; Foot, Bombay, India). Indian J. Environ. IIfaith 1971, 13(3), 171-81 J. S.; Simmons, II. L.; Ncwson, H. I,. (Mctcorol. Off.', Brack (ling). The safeguard features engineered into tlte Tarapur At. nell, Kngl.). Katun {Union) 1973, 244(5-118), 5-15-51 (Eng). Rower Station, India, to minimize, control, and monitor the The prodn. of NO, in tlie shock wave of explosions ttxsocri. witlt the testing of nuclear weapons ill the atm. is tailed, and compared with that expected from tlie engine exliniu>L products of fleets atm. pollutant* from the station are described. The limits prescribed (or the effluents from tlte station are discussed. The y spectra of- tlte effluents are presented and the radioactive of Concordes, each flying in the stratosphere for >10 hr every isotopes are identified. The majur constituents of the gaseous dny of tlie year. NO, injections into the stratosphere from past waste ore isotopes of Xe and Kr. Data on actual relenses are nuclear explosions ore cquiv, to large ns. of fully operational given. Tlte max. value of activity released it very much las Concordes. Anal, of tlie Oj records reveal no detectable changes than tlie max. permissible release. in the total atm. 0> during and ufler |>criods of nuclear weapon 139492m Concentrations of uranium in the air of a uranium testing implying that massive NO, injections into the strato refinery and in urine of the worker*.- Okabaya.->hi, II.; Jflki, sphere do not upset the Oj layer. S. Braverman M.; Watunabe, S.; Ilongo, S.; Suzuki M. (Natl. Inst. Radiol. 18948M1 Spectreshotometrie determination of small amouati Sci., Chiba, Japan). Uoken Butsuri 1972, 7(3), 151-5 (Japan)., of dimethyl sulfate in air. Toraezyk, Ihmuta; Bajerska, J ad- U conens. in the refinery sir were 0.6 X ID"0 to 3.7 X in-* g/l. wig* (Osrodek Badaw.-Rozwojnwy Przcni. Barwnilcow, Zgicrz, and in the urine of the workers, 3.9 X 10"* to 3.fl X 10"* g/l. Iol.). C/icnt. ,lnef. (n'arsav) 1973. 18(3), 543-9 (Pol). MetSO The concn. in the air wns higher than background by an order was absorbed in pyridine, A'-mcthylpyridinium sulfate heated of 2-4 and that in the urine was higher than that in the general with 0.2iV NaOH. and the resulting glutaconnldehyde (I) was public. These high levels in the urine are due to initiation of reacted with PhNlhat pH 6.5 to form I dianil whose absorbance higher concns. of air borne U In the working environment. was measured at 484 nm (molar absorptivity 3.4 X 101). Tha 139493a Work hygiene and effectiveness of health treasures method permits the detn. of 0.15 'v(Cfli)*S0/1. of air with an la the manufacture of chlorinated texpenes. Ashirova. S. A. error <5C-(. A. Janowski (USSR). Nanek. Tr. Leningrad, Cos. Inst. Vsaversh. Vrachei 139487p Emission spcctrographic determination of non- 1971, No. 9R, 2R-3Q (Russ). Chlorinated terpenes are widely homogeneity of laboratory samples of industrial dusts. Kokosz, used as insecticides. For agricultural purposes aquutic emulsions Andrzej; Grajpel, Andrzej (Univ. Jncielhmski, Cracow, Pol.). of polyclilornpinone (1) and polychlorocamphene (II) nre used. CJwm. Anal. (It'erMw) 1973, 18(3), 593-8 (Pol). Dust samples Titcy are toxic to humans, penetrating tltrough respiration and were fused with KStO? and Co>0 (internal std.) ami excited in alimentary canal* and skin. The max. permivdbie concn. nf nn n.c. arc; anal, lines were: Pe I 297.3, Zn l 334.5, I'b 1280.2, I-and II in working areas is 0.2 mg/ni*. Campltetie, a-pinene, I. Si I 288.2, A1 j 308.2, Ca ( 364.4, Mg it 280.3, Mn I 279.8, II, and Cl were detd. in 3180 samples of factory air. For 90% Cr it 284.fl, Cu I 327.4, and Co I 308.7 nm. NonhomogeneKy of the samples tlie eonen. of I was 1.5-10 times greater titan the was judged from the scattering of the relative intensity of tlie max. permissible concn. Dnring II prodn. the max. permissible anal, line* of the elements and internal std. The method was concn. was exceeded & or A-fold. The akin penetration rate used at random to investigate dusts from the marten steel was high. The skin of employees in 3 factories was studied. smelting process. A. Janowski By shift end, the concn. on uncovered parts of the skin and I39488q Exhaust amission of the Otto engine during knock esp. band* was 0.3-10 tng/dtn*. The highest concn., 2.9-109 ing combustion. Eberan-Eberhorst, Robert; Gruden, Dusnn; mg/dm*, was on the skin of operators pouring tlie II. The Schwarsbauer, Gerd (In*t. Verbrennungskraftmasch. Krafl- covered ports of skin had a concn. of 0-0.4 mg/dm*. Medical fahrwes.. Tech. Hochsch. Wien, Vienna, Austria). Erdatl examinations show that 1/3 of the tested workers had nervous Kohlt, Erdgat, Petrorkm. Brtnnst.-Chem. 1972, 25(12), 715-18 system changes and 2/3 had subtrophie and atrophic respiratory (Ger). Varying air: fuel rate, compression ratio or Ignitipn organ changes. J. Susrbka advance produces engine knock and also influence exhaust emis 139494p Effects of asbestos on the pleurae. Lesobre, R.; sions. Combustion temp, raises with knock as does the N0- Hagengut, A.: Ltgrand, M. (Cent. Pneumol., Hop Deaujnn, ttntHioa. The hydrocarbon emission i* lower with knock than Clieby, Fr.). Novo. Freest Med. 1973, 2(22). Fr`>. normal, providing air: fuel rate or ignition changes have caused 1 Thoraeotomie and necroscopic findings following exposure to knock, but higher due to changes of the compression rate. The asbestos dust are described. The primary lesion, tli* hy.ilin HCN-, HCKO- and Oremission*, a* well as odor, increase pleural plaque, devoid of clinical symptoms, seldom contain- rapidly with knock intensity. Knock Intensity 0-100 was any asbestos fiber. Later these plaques become calcified and th-. measured with the standard knockmeter in the ABTM-CFR-Test calcification continues even after termination nf.asbestos expo engine (MOZ). sure. In 15-20 years some workers developed pachypleuritis. 139489r Accurate measurement qf cadmium in airborne Benign recidiving exudative pleuresia caa be produced by asbestos particulates. Zdrojewski. A.; Quickert, Nr.; Dubois, L. (Chem. dust; this may develop into mesothelioma,- esp. witlt exposure Div., Environ. Prat. Serv., Ottawa^ Out.). Int. J. Environ. to droddolite. Geo. Sag Anal, Cktm. 1973,2(4), 331-41 (Eng). The accurate and rapid 130495q Technical progress in the chemical industry arid measurement of Cd in airborne particulates Is described, with industrial hygiene. Arkhipov, A. S.; Marchenko, E. X.; particular ref. to high-vol. air samples collected on glass fiber Filatova, V.S.; Egorov, Yu. L.; Novoselova, T. I.; Martynova. niter*. The method involves the acid digestion o! a part of A. P. (Tseatr. Inst. Usoversh., Moscow, USSR). Gig. Tr the filter followed by anal. using at. absorption sjiectroscopy. Pro,f. Zabol. 1973, (8), 1-5 (Russ). The effective use,0f equip The use of both conventional flame at. absorption and at. absorp ment, architectural design, ventilation methods. n^ste rcryi-iing tion with a graphite furnace atomizer was studied. Tlte Cd and fmul treatment,,ami measurement und eliiiii.. nioi; of lunici, detns. were free of matrix error: Small interferences by other gas, anti aerosol emissions are described. H. Sim-Ihtc elements could be accurately taken into account. For n typical 139496r Hygienic evaluation of working condition! in sr.me, air sample of 2000 m', the Cd amt. can be detd. with accuracy chemical plant* operating in the open air. Filatova, V. d : down to --2 ng/m*. Detns. below this level are possible at tlte Vinogradova, V. K.; Bashkirova, L. S. (Inst. Gig. Tr. iTorexpense of the speed and simplicity of the method. The possi zabo!., Gorki, USSR). Gig. Tr. Prof. Zabel. 1973, .St, 8-11 bility of a non-uniform distribution of Cd on the exposed filter, and the chance of accidental contamination of the filter or prepd. sample by tmtaa ul Cd am idbo dlwuiwl. (Russ). Key technol. equipment at 2 new N'H, and vinyl chloride plants were placed in the open air. - Working conditions and Industrial hygiene at these plant* or* satisfactory. 139490] Fossil fuel burning and carbon dioxide. Pesai; mistic view. Pyikowics, R. M. (USA). Commons Earth Sei., E. Strossber^ 139497a Changes in working conditions and health sums Gtnpkys. 1972..3(1). 15-22 (Eng). From Nuel. Sei. Abslr. of workers ef oil refineries in connection with technical progress. 1973, 28(3), 5602. The impact of the combustion of fossil Mukhametova, G. M.; Polyanskii, V. A.; Sukhanova. V. A.: fuels on the CO, coacn. in tlie atm. and tha consequences for . Mustaeva, N. A.; Ostrovskaya, R. S.; Braginskaya, L. L.* Earth ecosystems are postulated. The rat* of fossil fuel con Malur'eva, L. M,; Gazizov, M. P.; Emery*'1*10''11- A. P sumption increased an av. 4.5%/yr during the post century (Inst. Gig. Pofubol., Ufa, USSR). Gig. Tr. Prof. Za'.-.-l. 1073. causing a steady buildup in atm. and oceanic CO,. Mechanisms (8), (Russ). A comparative anal, examns. o! worker- n* by which CO, i<> removed from the atm. and sea water by oceanic the Ufn refineries mtuie in 1957-00 and 1070-71 ilu>u< ! sedimentary processes are reviewed. Caicos, show that by nUnlial (uvurahle ulunges in health (with a service rccdt-l u>< i. 1906 man had produced and injected into the atm. 05 times 10 yr). Tiie itumlwr >f iwraon* witli astlteuic ami ncur.i.^tciiK the CO, rcinoved by oceanic tedimenlory prucesi**, which are syndrumes, liyinu-nMua, anemia. tliruntWytoixni.., uml primarily tangeiuc. If CO, levels in the atm. continue u> vlifcnuc gasuiUs greatly declined. Some functionul cluiTa.-tcri>- increase catastrophic- warming of the surface of the earth will tics of tlie liver improved. Persons exaind. in WTlKl still Iwl AP0000867I Acute Inhalation Toxicity of Vinyl Chloride to Laboratory Animals E. MASTROMATTEO, M.D., A. M. FISHER, Pb.D.t H. CHRISTIE, B.Sc., and H. DANZIGER, M.D. Division of Industrial Hygiene, Ontario Department of Health, Toronto, Ontario; Department of Physiological Hygiene, School of Hygiene, University of Toronto, Toronto, Ontario; and Welland County General Hospital, Welland, Ontario Introduction scribed as very' slight. Elimination from the body FkAnbotdhoagmhneaalarufdpeeeNeogrsvseIctlrnepweaathlRooAoridwennrneimmptSmaeciidi.itenrnigtlepTaeheAhdieaHrtltvraipsuxorhniiyipdctnnVrposdehatgoaertldotuaueoneeiprdpnellsipcsireuaiionnnaelanitayroCelilcigstsoyirdsnirdefoetbmetnoaouvsdnhsaewftsoif.efosnaaeehtmf5eriornfzlyrniei<0iesnctaoalpniL0ranaerdAlceo-dcttataesmhppai$wleocyrolplo$aaoeayunovfmro3strsttiteiiedeonseftnf<dasorifydteYwei.Gtoceattl.a.ahhuixxOmlaoclTs,Ieeipiityvhttnceohhoiegleelleidnosioatwassrsycuwornsstwaw.iharmopedtmesebolAoiclveodetaelructehierarkhsnnrTusircrdeehae,ttierhamatseerrrniisrdeeitsldednodwefdhhsounsIeiibeeanash.itroinnsneysdelslTheworocwaitc1twmpnhaioe2onfrraeocretosVsneseSsrpouindekscseiceolantuvaidet.hdorrhwncenoyndaCbnbeceddlrftiiuseerttnyedasiahecmcydancenrthrrooraittaavvasaoelugroeedwagnepnrddstndrcaennnisoiidaodm*nadswmuid.onettniwooal.ietoducTiyfggsOraoithsehthbseathrlascspoeele.rteasiutiayssnreeovbhsdlAettiaxrnbexhh5luawecpraenomamndfcehneoaluogotooiartnodiuaectfgcoigrnhmnrsstdhasieosoitcdsvietthnuuniruoaeisinstsndetsgtdolfnhety.uwaicqeaedaarimltvuapaeodotsiaisosetndfyevhrnetareaoesetiSoitrsnhnmd*cphicdorccenamyeeteaahueotraltnilahsidiesrnttcmptoueeei.xehiuonmsdfditeMxlsmnartwolpeaesntiatirhvoiuencmrabiandeaernerrilrndieikieed,r,tr while cleaning out a polymerization vessel and they other while working in a pit. Details of these two fatalities have been reported by one of the uuL tbor*.1 I Published information on the acute toxicity of vinyl chloride to experimental animals proved scanty. Patty, Yanfc and Waite* in 1030 reported on the acute response of. guinea pigs to vinyl chloride. They found that exposure to 20 to 40 per cent in air killed guinea pigs in n short time; exposure to ten per cent was endured for several hours. Congestion and edema of tho lungs with hyperemia of the liver and kidneys were noted. Peoples and Leake* found the minimal anes thetic range of vinyl chloride in mice cxjxjsod for ten minutes wns S to 12 per cont in air; 25 to 30 per cent wns fatal in ten minutes. Dugs and rab bits were anesthetized within one minute when as an anesthetic agent. f Two cases of vinyl chloride gassing occurred in a factory in Great Britain where polyvinyl chlor ide resin was being made. These were reported by the Chief Inspector of Factories.* In one case a process worker was standing outside a polymeri sation vessel and washing it with a water stream. After ten minutes of this he suddenly collapsed across the open manhole. Artificial resuscitation was successfully applied. Subsequent symptoms experienced by this worker were tightness of the chest, nausea, abdominal pain and headache. The second case occurred in a maintenance worker who was overcome while repairing a vinyl chloride leak. Both men were hospitalized. Another worker whoso hands were accidentally sprayed with viny| chloride liquid under pressure developed a bum. This latter case was reported by Harris.' exposed to a concentration of nlxiut 18 per cant Such bums may occur with other highly volatile In air. Recovery wns very rapid with no apparent materials when xprnyed onto the hunds as a liquid untoward effect oven after prolonged anesthesia. under pressure. Lehmann and Flury4 noted tlmt vinyl chloride Filatova and Grniifdwrg1' in 1057 reported on was highly narcotic hut hud a wide margin l>c- hygienic conditions in :t polyvinyl proccswim tweon it* narcotic and Ictluil concentrations, its plant in the U.S.S.H. The plant was engaged in local irritating olivet and its toxicity were de the polymerization of vinyl chloride by mentis of m. AP00008672 Industrial Hygiene Journal 393 :t catalyst, Air sampling was done nnd tho con centration of vinyl chloride was found to vary from uboutSO ppm to nlxnit 315 ppm. (U.S.S.R. Threshold limit Value: 1 mg/litcr or about 400 ]^nn). A KpuKtic type blood vessel disorder was dcscriU'd in workers from tius plant. Bccuuxc of the recent fatalities mentioned above it was decided to undertake ocuto iuhalation studies in laboratory animals with commer cially available vinyl chloride. This was done both for comparison with earlier work and to provide information on the scute response and pathologi cal t'linnges in more than one species of laboratory animal. Experimental Materials and Procedures T'tnyf Chloride: The vinyl chloride was supplied in a metal cylinder under pressure. A commercial grade with the foliowing specifications was used: Spifla f>Tir it so*0 Wat*r eostaat Boiltaf-a--*C Hydrag-- iblwM* eoata-- AMtyteM AMtaldebyds Iroa Putty (paraaot) tmpwitix aM) o.sa-o.ow Nana . --U to --0J Non tO ppn "miTMTM SO PPB DWtrstUl EuitUlylim Im MS maximum *0 maximum Pure vinyl chloride is & colorless gas at room temperature; its boiling point is --13.0"C. It has a sweetish odor. Its flash point is given at --7S*C and its explosive limits in air from 4 to 22 per cent. It has the molecular formula CHjtCHCl. Animals: Mice, rats and guinea pigs were used in the study. Test and control animals were taken from the same laboratory stock. All were fed a standard commercial diet and housed in the same way. All were of the same stage of development. Equipment: The inhalation chamber capacity was 36.6 liters. It was equipped with a viewing window and an inlet tube. Vinyl chloride was re leased in gaseous form through an adjustable valve on the top of the containing cylinder, then through connecting rubber tubing and a recali brated Fisher flow-meter. Fresh air was pumped by motor at an adjustable controlled rate through a meter. Tho streams of air and vinyl chloride wore combined at appropriate rates of flow by a glass Y-tube leading through further rubber tub ing to the animul chamber inlet, to deliver a continuing stream in the desired proportions. Calculations and adjustments were made to pro duce the following flow concentrations of vinyl chloride in air for delivery: 10, 20, 30 and 40 per cent. All concentrations are expressed in this re port as per cent by volume in air. No determina tions of vinyl chloride concentrations wore done in the. teat chamtar during tlic experiments. Experimental Procedures: Different groups of five mice, five rats nnd five guinea pigs were placed in the chamber nnd exposed for thirty minutes to concentrations of 10, 20 and 30 per cent vinyl chloride in air. Simitar groups of con trol animals were maintained but not exposed to vinyl chloride. At tho end of thirty minutes ex posure the test animals were immediately re moved to fresh air. An additional group of five guinea pigs was exposed to a concentration, of 40 per cent vinyl chloride in air. In all 65 experimen tal animals were involved. Observations were re corded on the animals during and after exposure. The animals which died either during the ex posure or after a delay period were autopaied soon after death. Two weeks after the exposure sur viving test animals and controls were by a blow to the head with the exception of four control rets which were killed by ngtiM--rim- All animals were examined for gross pathologi cal changes. Tissues were removed from all ani mals for microscopic examination. The lungs, liver, kidney and heart were removed in all cases. The brain, adrenal, spleen, trachea. Lymph nodes, and the eye were removed from represen tative animals in each group for study. The tissues were preserved in formalin and sections made for staining with hematoxylin-eosin. Special stains were made where indicated. Observations Control Animals: No symptoms were exhibited by the 15 control animals and no deaths occurred. Ten Per Cent Vinyl Chloride: The response of anuruilS expds&Tto uus concentration is recorded below in summary fonn: Exposure Tlau {minus) 1 * to a so u so nupo-- Sight irritation la adea sad nta tnoraaaad motor activity first te nia, tfa-- im end stria-- pi--. iMMMd motor aeUvity la all *)au; MrftcUu of aatraaitia la sake. Piwxiixd Mm, wtwdr --1* aad mcacularincoordination in oil irriaa Mka and reta im Mda petition; nuarakr ifieeecdantioa, tanoets sad twitddac ot oUmnitki ia pda-- pi--. Mlea and rota unoonoeiaua. tultua pi-- my oaataady but atilt ataadiae. Mka sad nUa ia daap Barak; idIm pi-- In tida petitionwithtwrarw om Exposure was stopped and the animals removed to fresh air. All recovered, within five minutes. Twenty Per Cent Vinyl Chloride: The response 396 October, i960 oi animals cxiwscd to this concentration is re corded below in summary form: Eipniura Time (minute*) 1 0 It"! SI M SeipODW Immediate irritation in mice and rat*. MuaeuUr ineoonttaatioa in mloe and nta. Miceand rata on tbetrtideewith marked UWmui toiiekhd of the utiami* Mini Unetradlam and muscular inoo* ordianUno Is guinea pics. Mien aad ista unoonsdeui with rapid ImftuUr btanthlac; guinea pigs an* eoaerieoi butehavrin* msrksd twiteIvlag. Allaalsialeisdssp Mtcceie; reeplmtton* inagukr and rapid. Deep naraoete; breathing etow aad shal low. Breathing esassd in oaa mausei frothing at moutfcand nostrils in mieaand rata, All animals ia deep nareesis. Exposure was stopped and the animals removed to fresh sir. Mice and rats recovered faster than the (uilwa pigs and appeared normal within five mimiMk except .for one mouse which was dead. The guinea pigs continued to show muscular in coordination, unsteadiness on their feet until 20 minutes after removal from exposure. TAtrtir Per Cent Fifty! CAlorvie: The response of animals expound to this concentration is recorded below in summary form: npoMaTiSi <ashMWS> llsipanss MM stowsd Irritation immediatelyand tba Iota abowsd irritation quickly ttdtow. 1 MuMIlto InaoordiBatioa la miea and rate. s Mloa aad rats in sids position with marked tiwwa snd twitching 0( the oxtraaritiee; muscular ioeocrcUMtioa ingulIM pigs with developing pamly* sis of the auremitita. AH animal* UMonssioust rapid irregular raspimtioas in miss and rats with frothing about nose snd mouth; twhabihg of latieiriittee still occurred CW'nneHy in guinea piss. 10 Baapbattau stopped in mice; breathing slow aad ahallew in rate; guinea pigs atiH exhibited eeeaekanal twitching tnoeamenta oi the ratnrmitia. IS Braathlng auppod in rats; respiration* slow and shadow Is guinea pig*; twitching at eitrlmlties still ertstnt in guinea ptato Mies aad rata still: guinea pigs in deep narcosis with alow siwBov brcatUiae Exposure was stopped and the animals removed to fresh uir. The mice and rats were dead. The guineu pig* took 25 minute to return to their normal uppennuiro and activity. One guinea pig from this group died within 24 liount following ejqKisure. Table I Kumbor of Dcathn in Different Groups of Five Mice, Rats and Guinea Pigs Exposed for Thirty Minutes to Varying Concentrations of Vinyl Chloride in Air Ylnyl chloride canoes(ration (percent by volume In iu) Laboratory mlmil Mice Kata Guinea pig* Total IS 10 30 40 Total 0/0 0/6 l/ 0/6 s/s S/6 ---- 6/16 6/16 C/6 0/6 1/1* i/r 3/60 0/11 t/t* um t/t 14/10 AdetejraddeatHocaunad withinit hMmfcUmsingsntieaai*. Forty Per Cent Vinyl Chloride: Only five goinca pigs were exposed to this concentration. Signs of irritation were immediately apparent. Muscular incoordination appeared within seconds. After five minutes nil thu guinea pigs were unconscious with slow shallow breathing. At the end of the exposure period one guinea pig was dead and tha remaining four wore in deep narcosis. These sur vivors took 30 minutes to return to their normal appearance and activity, but one died within the following 24 hours. The number of deaths occurring In different groups of five laboratory animals exposed for thirty minutes to varying concentrations of vinyl chloride in air is shown in Table I. Pathological Findings Gross pathological and histological studies were carried out. The findings are summarized below. Control .4nima/i; These animals showed no gross or microscopic evidence of damage. Ten Per Cent Vinyl Chloride: All test animals survived this exposure. They were sacrificed two weeks later. On gross examination there was evi dence in mice of slight hyperemia of the lungs. Tliis was less marked In rats. Guinea pigs shout'd no difference from tofitrol animals. On liUtologic examination the mice showed very slight engorge ment of the pulmonary vessels. Onfe mouse showed dfgcncmtivo changes in tlie tubular epi thelium of the kidney with hydropic swelling. Thd rata showed slight congestion of tlx* capillar ies in the lung. Lungs in the guinea pig were also slightly more hyjx'remic than those of the control animals. Tiocnty Per Cent Vinyl Chloride: One mouse died ns n result of 30 minutes ('X]M>Htirc to this concentration. Pulmanury iTingextum tvtur evidtnit on gross examination. Microscopically, then* was Industrial Hygiene Journal 397 ('imi'rjff'tm'nt of tin* blood vessel* in the lung with ficed two weeks after exixmtre. There wns a Itntcliy ureas of atelectasis and minimal edema. tendency for the blood to remain unelntted in the Tin* kidneys showed minimal degenerative animals dying during rx])oxun\ This feature nm etiniigctf in the epithelium of the convoluted tu- noted in tlu- two hunmn fntiilitk* mentioned ear httlcy. lier but it is not a atxcific characteristic of vinyl 'Jin* test animal!* surviving this exposure were chloride. ssieritietxl in two weeks. On gross examination, congestion of the lungs was present in oil species, Discussion Imt it wax more marked in the mice and rats than in tin* guinea lngs. Histologically, there was evileiwv of pulmonary congestion m exposed ani mal*. .Some fatty infiltration was present in the The response of guinea pigs to inhalation of vinyl chloride was similar to that reported by Potty, Yant and Waito. ific guiiicu pigs brgflr liver of one rat. All other tissues studied appeared normal. Tiiirtg Per Cent Vinyl Chloride: Gross examina ^tmlyi hmu U1!, Ullcratod a greater exposure dur ing the experimental |>eriod than either the mice or rats. The results in mice agreed closely with tion of the animals which died as & result of this exposure revealed congestion of the lungs with those reported by Peoples and Leake. Rats were similar to mice in their response. hemorrhagic areas. The mice and rats in addition showed congestion of the liver and kidney. The one guinea pig death was delayed. In this animal The pathological changes in animals which died as a result of exposure were mainly those of vascular engorgement of the lungs with hemor there was marked congestion of the lungs with hemorrhages and the liver was distended and very rhages and edema. The severity of these changes varied with the severity of exposure. In the higher friable. The microscopic changes in the animals concentrations, pulmonary change was marked which died included marked engorgement of the imlmonary blood- vessels with edema and hemor rhages in the lungs. The trachea of one rat showed superficial desquamation of the epithelium. Congostion was also evident at the liver and kidney of the mice and rats. Hie liver of the guinea pig which had the delayed death showed severe fatty degeneration of the 'liver confirmed with frozen sections stained with Sudan III. The four surviving gumee pigs were sacrificed two weeks later, Marked pulmonary congestion was present with hemorrhagic areas and edema. In one case there was evidence of secondary bac terial infiltration. The 'liver in these guinea pigs gave the appearance of fatty infiltration but no fat was demonstrated on frosen section. Forty Per Cent-' Vinyl Chloride: One of five guinea pigs exposed to this concentration died during the exposure and' another died within 24 (tours. The two whieh died showed marked con with severe damage to the tracheal epithelium. Congestion of the liver and kidneys also occurred in test animals. These changes are similar to those reported by Patty and associates. Evidence of pulmonary congestion was still present in surviving animals sacrificed two weeks after exposure. Patty and associates reported that such changes had disappeared in about eight daysDcgenerative changes in the tubular epithelium of tho kidney were noted In one mouse dying as a result of exposure and in one mouse sacrificed two weeks after being exposed. Such changes, however, were minimal and not shown by other animals in the group. One of the guinea pigs with delayed death fol lowing exposure showed severe fatty infiltration of the liver. Changes suggestive of fatty infiltra tion were observed in other exposed animals but not confirmed by frosen sections stained with Sudan III. gestion of the lungs with?'hemorrhagea on gross examination. This waa-aho'evident on micro- Summary wopie examination. Hie User of one of these gave Separate groups of laboratory animals com the appearance of fatty'infiltration, but no fat prising five mice, five mts and five guinea pigs could bo demonstrated on frosen section. were exposed in an inhalation chamber to concen The threw Burvrvinggulnea-pjgs were sacrificed trations of 10, 20 and 30 per vent viuyl chloride two weeks later. Maricod congestion of the lungs in air for 30 minutest An additional five guinea with hemorrhage was evldent'on both the gross pigs wore oxpoeod to a til per cent concentration and microscopic examination. In ono guinea pig, for a similar period of time. Tha response byeach the tracheal epithelium 'was complctdy sbsent. of the three qwcics to these concentrations is Pathological studies Which were nisdo of the noted. Itrnin, heart, spleen, adrenals, lymph nodes and Inhalation of those relatively high concentra tlu> <>>(? showed no `difference between controls tions produced narcosis and death. Mice were the uiut animals dying tui a result of exposure or be most susceptible with guinea pigs considerably tween controls and the surviving animala sacri more resistant. Rats were similar to mice in their AP00008675 398 October, S$80 response. Exposure to ten per cent vinyl chloride la air produced deep narcosis in mice and rats but no deaths; 90 per cent concentrations killed mite and rats. Exposure of guinea pigs to 20 per east produced deep narcosis; hut three of five guinea pigs survived exposure to 40 per cent con centration. Animals dying as a result of exposure were aatopried shortly after death. Survivors and con trol were sacrificed two weeks later. Gross pathological and microscopic studies were dona and these findings are described. The principal pathological changes in animals dying from exposure were congestion of the lung! with pulmonary edema iwd hemorrhages in some, nod congestion f the liver and kidneys. Failure of the Uood to clot was also observed. One of the three guinea pigs which died following exposure showed severe fatty infiltration of the liver. fiumvors sacrificST showed Uttde difference from the control animals. Pulmonary congestion was still evident but liver and kidney congestion was not. References I. DumoM, K.: AeddcaUl PeMoaiac bjr Vtayf OUhMis S> part ai Two Can OntSni JIW. 1m. j, tt: SM (April MW). 1 J, PjHnr,F.A,W.F.YMfflieC.P.ViiniAflaRiipoiM a! OuIm Pi** lo Vapen of S^m* TTrr rnmniwSaf flm nin CuspMBdi V. Vlayl OUorida 1 HttOk M*L U: IMS 3. FWH.M. A. a. amC. D. Loaest Th AffttriB AetiM d YfayiChfcxtio, /. Pkvmietlm 4* IH (IMS). 4. Lihhmw, K. B.. A F, Pim: Tttimlmt IwAw H tmuMHA Stlmui, TnaOkMd by E. Uniul B. f. Smith, Jr., WIDUap A WiltdM, BalUmora (1HD. I. 8cKamiMM,0.:CtudfeqrLhmiaa, K.B.aadF. flury. 9. Oku, R. X., C. J. Caw, J. C. Kumi, and M. J. Savss* vauu AoMtteai* XXVII. NiraA with VInyi CUorid*. AMAkofeUff i: 149 <tM7). 7. Ciu, J., &. If, BnaMN, J. F. Viiua*. aw J. C. Kaam Jat Aawthwia XXIV. Cbmatal QpnrtitsUoa el Hydre- eaifaeu And Cu4iu AntomatiSty, J. Piano. W Iirr. riMas.'i uw). liwiiJIeiittflii CM*f liufW *4 FaMriM hr Us Ttar Mi, Imlm t H.M.S.O. Cmd. *m (Umh IMS). D. X.: Hcttth Frobltn* Is th* Uutiwtim *s4 UmoC PtMUm, On*. /. /aA MU. Jfc US <1M. Flutter*, 0. fl., axd B. X Gsosunat BygUali WarUn* CaaditieM is Pelrviayl Chlorida Tat Plaata. vp. SMS (Jaanair 1M7). Atatnrad ih At* / WmU JM. U: N (July 1U7). Back Issues of AIHA Quarterly FOR 18 TEARS THE AIHA QUARTERLY was the publication of AIHA, then m 1958 it was changed to be the AIHA Journal with six issues per yeardnstead of four. How limitation of storage space is forcing us to dispose of all back Issues of thaQuarterly. For individual issues quantities on hand vary from a low of 8 upward. All issues except March 1946 (Vol. 7, No. 1) and June 1958 (Vol. 17, No. 2) are available as we go to press. Some issues are reprinted without cover. The cote is gliO pay issue ($1.00 each when more than three issues are ordered) from Volumes 7 through 18. From 1940 to 1946 AIHA Quarterly appeared as a section in Industrial Medicine and Surgery, however these issues of those sections have been reprinted and an available as a unit. The complete reprint, Vole. 1-7, costs $10.00 to members, and $50JD0 to companies or departments of government. Handsomely bound, ccanpiste eats of Vols. 1-18 with cumulative index are available for $250.00 per set. Address all orders to: George D. Clayton, Executive Secretary, AIHA, 14125 Frevoet, Detroit 27, Michigan. (Back issues of AIHA Journal, 1958 to present, may be obtained directly from our publisher, The Williams is Wilkins Company, 428 E. Preston, Baltimore 2, Maryland, at $1.50 each issue.) AP00008676