Document DvMVLB1rv40yONxOwKYY1pq14

TOXICITY TO AQUATIC PLANTS TEST SUBSTANCE Identity:A mixturecontainingperfluorooctanesulfonatmea;y alsobe referredto as PFOS, FC-95, or as a component of FC-206CF or FC-206CF-X. (1-Octanesulfoniacid)(CAS # 2795-39-3). Remarks: The 3M productionlotnumber was 101. The testsample isFC206CF. Currentinformationindicatesitisa mixtureof0.5% PFOS, 10% diethyleneglycolmonobutyl ether,78.08% water,1.375% acrylicfoamer, 1.5% sodium octylsulfate1,% sulfonatedalkylethersalts,6.0% urea,0.5% triethanolmaine, and 0.05% tolyltriazole. The followingsummary applies to a mixture with incompletely characterized concentrations of impurities.Data may not accurately reflectthe toxicityof the fluorochemical component of the testsample. METHOD: Method: OECD Guideline201 Type: Acute Static GLP: No Year study performed: 1991 Species: Selenastrumcapricomutum Source: In-houseculture.Originallfyrom theCultureCollectioonfAlgae at the Universitoyf Texas atAustin,Texas. Element basis: Algalcellcounts (cells/mlg)r,owth rate Exposure period: 96 hours StatisticaMlethods: Statisticanlalyseswere performed usingthe computer program ofStephan, 1983. Analyticalmonitoring: pH, conductivitya,nd temperature Test Conditions: Algal NutrientMedium: Nutrientmedium per U.S. EPA, 1978. This nutrient medium providedallmineralnutrientesssentialforalgalgrowth and also served as the diluentforallalgaloperations.The pH ofthissyntheticalgal medium was 8.0. Stock and testsolution preparation: A primary1000 mg/L stocksolution was prepared inalgalmedium, and added directltyo the exposure vessels. Exposure vessels: 250 mL Erlenmeyer flaskscontaining100 mL test solutionand capped withinvertedglass beakers. Agitation: Continuous Number of replicates:3. Initiaclellloading: 1.0 X 104 cells/mL. Number of concentrations: fiveplusa blankcontrol Lighting:Continuoulsightiantg44 pEin/seC/Mu2sing cool-whitefluorescent lamps. Water Chemistry: pH range (0 - 96 hours): 8.0 - 10.8 (controlexposuie) 8.1-8.3 (1000mg/L exposure) Test temperaturerange (0- 96 hours): 21.9- 22.3'C(incubator) RESULTS Nominal concentrations: Blank control6,2.5,125,250, 500, and 1000 mg/L 96-Hrs.Algal Growth Response values Nominal Exposure Conc. (mg/L) Control 62.5 125 250 500 1000 Cell-Count (cells/mL) 602,000 632,000 353,000 304,000 7,000 0 Avg. Specific Growth rate 0.043 0.043 0.037 0.036 -0.003 0.000 % Change from Control --0 14 16 >JOO >100 Element values: 96-hour ErC50 = 254 (233 - 277) mg/L 96-hour NOEC = 62.5 mg/L Element values are based on nominalconcentrations. Reversibilityof Growth Inhibition:Not conducted. Remarks: Testingwas conducted on the mixtureas describedinthe Test Substance Remarks field.The values reportedapply tothatmixtureand not the fluorochemicalproportionalone. CONCLUSIONS The testsubstance exhibitsa 96-hour ErC50 of254 mg/L witha 95% confidenceintervaolf 233 to 277 mg/L. The 96-hour No Observed Effect Concentration(NOEC) was 62.5 mg/L. Submifter: 3M Company, EnvironmentalLaboratory,P.O. Box 33331, St. Paul,Minnesota,55133 DATA QUALITY ReliabilitKyl:imiscrhankin=g 2. The studymeetsthecriterfioarquality testing.However, the sample puritywas notpropedy characterizedand the study lacksanalyticaclonfirmatioonf theamount offiuorochemicalproportion inthe solution. REFERENCES This testwas conducted by EnviroSystems Division,Resource Analysts, Incorporatedo,f Hampton, NH atthe requestofthe 3M Company, St.Paul, MN, Lab Request number J18844, 1991. OTHER Last changed: 6/28/00 Study Title Static Acute Toxicity of FC-206cr to the Alga, Solonastrum capricornutum Authors Timothy J. Ward Robert L. BoorL Study Completed August, 1991 Performina TaboratoiX EnviroSyst@ DLvLaLon Resource Analystlai lnc*rporated Ch@e LiLfayftte -4@ ftmptong' Rdni Ha@@, 03842 "SIT IJLMI 1. BUXKARY The acute capricornutum, is toxicity of described in FC-206CY to the alga, S*Ionastrum this final report. The test was conducted for 3M Company for 96 hours during July 15 to 19, 1991, at the EnviroSystems Hampshire. Division of Resource Analyst@, Inc. in Hampton, Now It was conducted by Jeanne Magazu, Peter Kowalski, Illon Stanford, Robert Boeri, and Timothy Ward. The test concentrations of was test performed substance under static conditions with five and a dilution water control at a mean temperature of 22.1*C. The dilution water was synthetic media prepared with sterile doionized water. Flasks were incubated on a rotary shaker. Light was adjusted to 44 uZin/ &*C/m2 with a photoperiod of 24 hours light and 0 hours dark. Nominal concentrations of FC-206CIP weros 0 mg/L (control), 62.5 mg/L, 125 mg/L, 250 mg/L, 500 mg/L, and 1,000 mg/L. Nominal concentrations were used for all calculations. Algae used in the test was from an in-house culture that was maintained under test conditions. Zxposure of algae to the test substance resulted in a 72 hour EC50 of 203 mg/L FC-206CY and a no observed effect concentration (NOEC) of 62.5 mg/L. and 62.5 mg/L, respectively. The 96 hour ZC50 and NOEC are 254 mg/L Page 2 of 15 Resou,=Arg** &j6kli@rdiMLL12M 11. TABLX OF OON-X NTS SECTIONs 1. S"@.ry ii. Table of Contents Ill. Index of Tables IV. Introduction V. Methods and Hat*rials Vi. Result@ VII. References Appendix A. Water Quality Data from Toxicity Test PAGE 2 3 4 5 5 12 13 @k age 3 of,.': z III. IKDEX OF TABLIS Table 1. Chemical characterization of a representative sample of doloniz*d water used to prepare test media for toxicity toot Table 2. Growth data from toxicity test Table 3. Percent change from control and average specific growth rate from toxicity test Table 4. Median effective concentrations (ZCSC&) from toxicity test Table A.l. Temperature measured during toxicity test Table A.2. pH measured during toxicity test PAGE 6 9 10 11 14 ]page 4 of 15 7,, Ruou" W- IV. INTRWOCT10K This study was sponsored by 3X Ccu%wLny, at. Paul, Minnesota. The objectlv* of the study was to determine the acute toxicity of FC-206CY to a freshwater alga. The r*port contains sections that describe the awrthods and materials employed in tne study, and the results of the investigation. The report also contains an appendix that presents the water quality data collected during the test. TEST SUBSTANCET V. K M ODS AND MATERIALS FC-206CY (EnviroSystems Sample Number 29389) was delivered to ZnviroSysteme on May 23, 1991. It was contained in a 4 oz. plastic bottle that was labelled with the following Lnformationt IJ1884-4, PC-206CF*. FC-206C? (a light brown liquid) was supplied by 3M Company, 935 Bush Avenue, St. Paul, Minnesota. Prior to use the test material was stored in the dark at room temperature. A reserve sample (approximately 1.0 gram) will be retained at ZnviroSystems for a minimm,-of 10 years. DILUTION WATER: water used for acclimation of tout organime and for all toxicity testing was sterile approximately 8.0. sample of deionizod Table 1. enriched media (U.S. EPA, 1978) with a pH of Results of chmical analysis of a representative water used to prepare the media are presented in TEST ORGAMISMs Alga* used for the test was from a culture originally procured from the Culture Collection of Alga* at the University of Texas at Austin and delivered to Inviromystma on May 14, 1992. The identity of th* Culture was verified using an appropriate taxonomic key. transferred to sterile enriched media test and maintained at test conditions. The culture was identical to media used for this TOXICIT!F TZSTINGs A screening iont with the test substance 28, 1991. . NooLnal' concentrations wan conducted June 24 to 10 og/L, 100 of -th* test material w*res I ml at 1,000 mg/L, and mg/L was 1,000 mg/L.- After 96 hours the number of cell per al at all loss than 50% of the control and the number of lower concentrations were gr*ator than 50% of the contro 1991. TItheisdbeafsienditoinveprotcoexdiucreistyoftetshte wOEaCsD pe(r1f9o8r4m)e.d during July ls t Page S of 15 Table 1. Chemical characterization of a representative sample of dolonized water used to prepare test media for toxicity test Parameter Organochlorine pesticides Unit of Measure@nt ug/L Reporting Limit 2 organophosphorus ug/L 0.5 pesticides Polychlorinated ug/L 0.5 biphonyle Notot ND not detected at or above the reporting limit. Measured value ND mn ND ,4,A' M@ tw The test was conducted at & target temperature of 22 1 2*C with five concentrations of test substance and a dilution water control. A 1#000 mg/L stock solution was prepared La dilution water. The stock solution was added directly to dilution water contained in the test vessels without the us* of a solvent. Nominal concentrations of the test material weros 0 mg/L (control), 62.5 mg/L, 12S mg/L, 2SO mg/L, 500 mg/L, and 1,000 mg/L. Algae was randomly and equally distributed among three replicates of each treatment at the rate of 10,000 calls/ml. The test was performed in 250 ml glass Zrlonmeyer flanks that contained 100 ml of test solution. The flasks were capped with inverted glass beakers. Test vessels were randomly arranged on a rotary shaker in an incubator during the 96 hour test (a random numbers table was used to select the location of each vessel). A 24 hour light and 0 hour dark photoperiod was maintained with co@l-white fluorescent lights that provided a light intensity of 44 uRs-1m- . The number of algal calls/al in each test vessel was determined visually every 24 hours by mans of direct microscopic counts with a hemacytometer. The pH (Beckman model pHI 51 meter) was determined in each test flask at the beginning and end of the test, and temperature (ASTH mercury thermometer) was measured and recorded daily in the incubator. STATISTICAL METHODS: Results of the toxicity test were interpreted by standard statistical techniques (Stophant 1983), The binomial or moving average method was used by the author to calculate ZCSO values using nominal concentrations of test substance. Dunnett's test# The NORC was calculated using variance (ANOVA). which includes a parametric one-way analysis of the natural log The average specific growth rate was calculated an hours minus the of the number of cells per ml at 24, 48, 72, or 96 natural log of the number of cello per al at 0 hours divided by the exposure period. The percent change from control is calculated by subtracting the sample average specific growth rate from the control average specific growth rat** dividing that value by the average specific growth rate in the control and multiplying that value by 100. The values. percent change from control was used to compute ZCSO and Page 7 of 15 MILPM Vi. RZSULTS Biological and water quality data generated by the acute toxicity test are presented in Table 2 and Appendix A, reopocti-IY, and the percent of control and av*rage specific growth rate information is presented in Table 3. The 24, 48, 72, and 96 hour BC50a for algae exposed to FC-206CF are presented in Table 4. The 72 hour ZC50 is 203 mg/L, and the 72 hour NOEC is 62.5 mg/L FC-206CF. The 96 hour EC50 is 254 mg/L and the 96 hour NORC is 62.5 mg/L. rag* a at y 6&oddoyd Table 2. Growth data f. t*zLeLty test NomLnal ConcontratLen (mg/L) rep. 0.0 (control) 62.5 125 2SO 500 1,000 1 2 3 mean 1 2 3 mean 1 2 3 mean 1 2 3 mean 1 2 3 moan 1 2 3 moan M. r of Calls per KLIlLliter x 103 (hour) 0 24 48 72 96 10 28 128 310 620 10 28 116 246 S12 10 38 146 288 674 10 31 130 281 602 10 26 105 220 6S4 10 26 95 208 690 10 32 es 228 5S2 10 28 95 219 632 :@o 20 GS 84 354 10 24 55 216 350 10 22 60 110 356 10 22 60 103 3S3 10 20 so 214 426 10 28 35 74 218 10 22 35 70 268 10 23 40 86 304 10 20 10 20 6 10 10 10 a 6 10 12 24 4 a 10 14 11 11 7 10 10 a 0 0 10 12 14 0 0 10 26 a 0 0 10 13 10 0 0 Page@ 9' of 15 Table 3. Pfr*crncecthn&tftgs from control &M average spocifla growth rate oxialty test nominal Concentration of Test Substance (mg/L) Average or-ocific growth rate 24hr 48hr 72hr 96hr 0.0 (control) 62.5 125 250 500 1,000 0.048 0.054 0.046 0.043 0.043 0.047 0.043 0.043 0.033 0.038 0.032 0.037 0.035 0.029 0.030 0.036 0.014 0.002 0.001 -0.003 0.011 0.000 O.GOO 0.000 P*rcent change from control 24hr 48hr 72hr 96hr a 0 0 0 10 23 6 0 31 30 30 14 27 46 35 16 71 90 98 >100 77 >100 >100 >100 'We 20 Of 25 T&bls 4- zxposure period 24 hours 48 hours 72 hours 96 hours ffactiveconcentrationstscsoo)fre- t@o*icitytest zcso 332 mg/L 199 mg/L 203 mg/L 254 mg/L gs percent Confidence limits 279 403 mg/L 177 223 mg/L 183 225 ag/L 233 277 mg/L ralculation method Moving _%-r&90 mo,,.ng ?,Verage Moving hverlg* moving Aver&96 page f is 4 uico VII. RRTXRZNMB OZCD. 1984. OuLdelLnes for Te*tLag of Ch=Lcalo. softion 21 Xff*cto an SLotLc Systems. Method 201, Alge. Growth InhLbition Toot. Adopted June 4, 19a4. Stephan, C.E. 1983. Czmputer program for calculatlon of LCSO values. Personal communicatlon. U.S. EPA. Bottle Test. 1978. The Solon&xtrum caprtcornutum EPA-600/9-78-018. July, 1978. Printz Algal Assay page. 12 of IS t Itoootxcomo@fti=@&06@ci MLLFM Ammndix A. WATER OUXT-ITY DATA FRM TOXIUIX_= 77 &.V,Of 25 Ilk ic 40, Table A.I. To"rature measured durlng toxLeLty test Day of txposure 0 1 2 3 4 Tm"ratur* of Incubator,*C 21.9 22.1 22.1 22.0 22.3 ROMAN m*sti 'rableA.2. pn "&Surlod during toxicity test NOML"L concentration (Mg/L) 0 (Control) 62.5 12S 250 Soo 21000 ROPILeate 1 2 1 2 3 1 2 3 1 2 3 1 2 3 1 2 3 PH initial final 8.0 8.0 8.1 8.3 8.3 8.3 8.3 8.3 8.3 8.3 8.3 8.3 8.3 8.3 8.3 8.3 8.3 8.3 10.8 10.8 20.6 9.6 9.6 10.0 9.3 9.3 9.1 9.3 9.1 8.8 8.4 8.4 8.3 8.2 8.2 8.1