Document Dp1qGVqK3ymrqQxQDV9zpx1B

itSff \ 3M General Offices `y T t b l P f I- 0700-/3 7 %3M Center St. Paul, MN 55144-1000 65! 733 1110 July 12, 2000 M z a _ o ls ,'.i \ Dr. Charles Auer, Director Chemical Control Division Office of Pollution Prevention and Toxics United States Environmental Protection Agency 401 M Street, Southwest Room 403 East Tower (Mail Code 7405) Washington, D.C. 20460 Re: 3M Fluorochemicals Document Submission --CD Version Dear Charlie: Enclosed is the CD version o f the documents submitted on perfluorooctane sulfonates and related compounds to EPA over the past few months. This CD contains the information provided to the agency beginning with the April 20, 2000 and ending with the May 18, 2000 document productions to the agency. Included with the CD 's is an information sheet on how to access the documents and how best to use the search capabilities incorporated onto the CD. Several items should be noted before you access the documents: 1. This CD has an extensive index such that the reader can scroll down the index, find the document(s) he/she wants, and click and open that document. Note that even though the index includes documents submitted after May 18, 2000, those documents cannot be opened, as they are not on the CD. 2. No confidential documents have been put on this CD. The sanitized version o f any confidential information is on the CD. Due to concerns over confidentiality, we would ask that you refer to the paper copies already submitted to EPA when you seek to review confidential information. 1-- o-n ro <T> "-W -ar ' o n ;U 3. The CD contains the documents by submission date, just as EPA received them. Each separate submission begins with the cover letter used to convey the information to the agency and the indices that accompanied the cover letter. 4. The information provided to the agency contains information that 3M voluntarily has submitted as part of our continuing discussions with EPA regarding fluorochemistry. These documents are submitted for read-only purposes and are not to be modified. For security reasons and to discourage any form o f tampering with the documents, 3M will be maintaining a master CD and original paper copies o f the same documents that appear on the CD. In terms o f any discrepancies between the paper copies and the documents on the CD, the information contained in the 3M paper copies is considered to be controlling. FYI-00-001378 85000000030 FY I-0 0 -0 0 1 3 7 8 85000000030 OOOOOl Dr. Charles Auer . July 12, 2000 Page 2 I We hope that this form of the information assists the agency in the review o f the documents. Please do not hesitate to contact me at 651-733-6374 should you have any questions. Very truly yours, William A. Weppner, Ph D. Director o f Environmental, Health, Safety and Regulatory Affairs Specialty Materials Markets 3M Bldg 236-IB -10 3M Center St. Paul, M innesota 55144 E;mail: waweppner@mmm.com Enclosures 000002 3M Fluorochemical EPA Submission CD Adobe Acrobat Reader Quick Reference Guide O pening CD: Place CD in CD-ROM drive. The CD will activate by itself and will open the following dialog window: r 3Mrfcjoiot'hemicdlEPASubmissions CDNo. I 1 Instai Aciobal Road JaMo rfwjprwrw^en*ouoiwwPnirwuciecnon . C'.,* oC.,' , y... ................................. ` . , niim ' *,* O'* I V4 S, > - 4> > ' y ' * slSil " : '.v .y C * ' M* ' V 'y ' ' v, , jf . n* * `` , <f , 1 ' ; '' If your computer does not contain Adobe Acrobat Reader then click on the Install Acrobat Reader tab and follow the instructions. After installation is complete then click on the Run tab. The following Notice will appear: NOTICE: The information provided to the agency contains information that 3M voluntarily has submitted as part o f our continuing discussions with the EPA regarding fluomchcmistry. These documents are submitted for read-only purposes and are not to be modified. For security reasons and to discourage any form o f tampering with the documents. 3M will be maintaining a master CD and original paper copies o f the same documents that appear on the CD. In terms o f any discrepancies between the paper copies and the documents on the CD, the information contained in the JM paper copies is considered to be controlling. Click Here to Continue After reading the notice click on the arrow in the lower right hand comer. This will take you to the 3M Fluorochemical EPA Submissions index. At this point you are ready to search and navigate through the documents. 000003 O pening a Document: .The documents on the CD are p d f (portable document format) image files. 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Or you can pull down the Edit menu, select Search and then select Search Indexes: E _A_c_r_o b a t R e n d e r [E P A _ T fJC pdf] EDO Qocument aw Window |ep ii M ! ' +!Q 0 [ S i **4# : ..ut-gins] O ffic e SoteciAD ttrl+A . DgsetaetAB CUj*3Nft*A Eind... Otiti* JM Fluorochemical EPA Submissions . , OrftShifttF No.. 6 Prever pretini The in (PFO ofAssistant... CWtStG Ctrt+Shilt+W :) fertility, developmental and ludy o f PFOS in rats (Sponsor's study . mortality, ami cardiac myopathy in cotation mie effeet o f pcriluorooctanoic acid (PFOS) and clofthric acid__________ Dev el i o f genes involved in cholesterol biosynthesis and lipid transport m human and rat fetal and neonatal livers Final Report Protocol 4IK-OIJ Orai (Gavage! Pharmacokinetic Recovery Study o f PFOS in Rats (Sponsor's study No. T-0295.12) Protocol l-'ACT-TOX-l 10 Oral (Gavage) Pharmacokinetic Study o f PFOS in Rats. Protocol f inal Report Protocol 418-015 Oral (Gavagc) Pharmacokinetic Recovery Study o f PFOS in Rats (Sponsor's study No, T-6295. 14) Protocol FACT-VOX-111 Oral (Gavagc) Pharmacokinetic Recovery Study o f PFOS in Rats, Protocol Summary PFOS Rar Two-Generation Reproduction Study ( Study No 5M I - Argus 4 18-008 [in-lifel, FACT-TOX-OI2 [analytical)) ___________ f 39 I >l u l l 94! a It in i & S ta rt | -r*) Fori*it(RlSoftware Notas | ,,Titajah - ReHeclkmlot U N ..|[~(>) A cro b a t R eader - f... f l y Microsoft Wqtd - 3M Flu...j |< p P f i i P 000004 This will open the Index Selection dialog window. Click on Add. This will open the Add Index dialog window. Click on the mmm.pdx file. Then click on Open. , jLoakjn;. , ) -si 3m_epa1 (D ) L j 405 Plj mmm 'nuPMDFWs wmuui LZJxj 3 JB| JfsTj; Fiteaarni' . Fites of WW jAcrabat Index (".PDX] pert Cancel This will take you back to the Index Selection window. MMM should now appear in the box. Click on OK and you can begin searching. To start a search query press Ctrl + Shift + F on keyboard. This will open the Search dialog box: Adobe Acrobat Search Find Results Containing Text S e a rc h Clear r Options ~~ .. -..... ............. j F Word Stemming I" Jhesautus F Sourds Like SearcSgin~thMMM index. in d e x e s ... F M atch Case F Proximity 000005 Type the text you want to search for in the Find Results Containing Text box: The text you type in can be a single word, a number, a term, or a phrase. You can use wild-card characters (*,?), and Boolean operators (AND, OR, NOT) in the text box. Because of this, you must enclose any search term that includes and, or, or not in quotes. Select Search. The documents that match your search query will be listed in the Search Results window. Documents more likely to contain relevant information are listed first on the list. Double-click a document that seems likely to contain the relevant information. The document will open on the first match for the text you typed. 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Reduce image. 000006 CD No. 1 3M Fluorochemical EPA Submissions April 20,2000 ______________________________________________ _ ______ Cover Letter to EPA___________________________________________________ Protocol 418-008 Combined oral (gavage) fertility, developmental and perinatal/postnatal reproduction toxicity study of PFOS in rats (Sponsor's study No.: 6295.9), Table E20, 1-3____________________________________________ Prevention of fluvastatin induced toxicity, mortality, and cardiac myopathy in ______ pregnant rats by mevalonic acid supplementation.___________________________ The mechanism underlying the hyperlipemic effect of perfluorooctanoic acid ______ (PFOA), perfluorooctane sulphonic acid (PFOS) and clofibric acid._____________ Developmental changes in the expression of genes involved in cholesterol ______ biosynthesis and lipid transport in human and rat fetal and neonatal livers._______ Final Report Protocol 418-013 Oral (Gavage) Pharmacokinetic Recovery Study of ______ PFOS in Rats (Sponsor's study No. T-6295.12)__________________________ _ Protocol FACT-TOX-110 Oral (Gavage) Pharmacokinetic Study of PFOS in Rats. ______ Protocol_________________________________________________________ _ Final Report Protocol 418-015 Oral (Gavage) Pharmacokinetic Recovery Study of ______ PFOS in Rats (Sponsor's study No. T-6295.14)_____________________________ Protocol FACT-TOX-111 Oral (Gavage) Pharmacokinetic Recovery Study of ______ PFOS in Rats, Protocol________________ _______________________________ Summary PFOS Rat Two-Generation Reproduction Study ( Study No. 3M T______ 6295.9, Argus 418-008 [in-life], FACT-TOX-Q12 [analytical])________________ Report Amendment I, 13, Protocol 418-008 (Sponsor's study No.: 6295.9) Combined Oral (Gavage) Fertility, Developmental and Perinatal / Postnatal ______ Reproduction Toxicity Study of PFOS in Rats, Final Report___________________ Analytical Lab Report on the Determination of the Presence and Concentration of Potassium Perfluorooctanesulfonate (CAS No.: 2759-39-3) in the Serum and Liver of Sprague-Dawley Rats Exposed to PFOS via Gavage, Lab Report No. ______ <U2006>, Requestor Project No. <3M TOX 6295.9>________________________ Interoffice Memo from Tom Kestner to Leo Gehlhoff, "Fluorochemical Isomer ______ Distribution by 1QF-NMR Spectroscopy___________________________________ Final Report Protocol 418-008 Combined Oral (Gavage) Fertility, Developmental and Perinatal / Postnatal Reproduction Toxicity Study of PFOS in Rats (Sponsor's ______ study No. 6295.9)_____________________________________________________ ______ Summary PFOS Rat Cross-Fostering (Study No.: 3M T-6295.13, Argus 418-014) Final Report Protocol 418-014, Oral (Gavage) Cross-Fostering Study of PFOS in ______ Rats (Sponsor's study No.: T-6295.13)____________________________________ Analytical Lab Report on the Determination of the Presence and Concentration of Perfluorooctanesulfonate (PFOS) (CAS No.: 2759-39-3) in the Serum of SpragueDawley Rats Exposed to Potassium Perfluorooctanesulfonate via Gavage, Lab ______ Report No. U2779, Requestor Project No. 3M Tox 6295.13___________________ Page 1 000007 3M Fluorochemical EPA Submissions April 21,2000________________________________________________________ ______ Cover Letter to EPA ________________________________________ __________Radiolabel Oral Absorption and Intravenous Pharmacokinetic Studies in Rats ____________ Synthesis and Characterization of FC 95 ^ C __________________ Extent and Route of Excretion and Tissue Distribution of Total Carbon-14 ____________ in Rats after a Single Intravenous Dose of FC 95 ^ C_________________ ____________ Absorption of FC 95 ^ C in Rats after a Single Oral Dose_____________ Cholestyramine-Enhanced Fecal Elimination of Carbon -14 in Rats after Administration of Ammonium C] Perfluorooctanoate or Potassium ____________ [14 C] Perfluorooctanesulfonate___________________________________ __________Dermal Absorption and Intravenous Pharmacokinetics in Rabbits___________________ Single-Dose Intravenous Pharmacokinetic Study of T-6246 in Rabbits. 3M ____________ Environmental Lab Study No. AMDT-042095.1______________________ Single-Dose Dermal Absorption / Toxicity Study of T-6049 in Rabbits. ____________ 3M Environmental Lab Study No. AMDT020895.1___________________ Single-Dose Intravenous Pharmacokinetic Study of T-6049 in Rabbits. ____________ 3M Environmental Lab Study No. AMDT-112294.1___________________ __________ Teratology Studies in Rats and Rabbits_______________________________________________________ ____________ Oral Developmental Toxicity (Teratology) in Rats and Rabbits__________ Rat Teratology Study T-3351 Final Report Hazelton Labs America Project ____________ No. 154-160___________________ Letter to Mr. William C. McCormick, III from E. Marshall Johnson, ____________ reviewing draft Rat Teratology Study T-3351_________________________ Oral Teratology Study of FC-95 in Rats, Riker Lab, Experiment No. ____________ 068TR0008____________________________________________________ Letter to Franklin D. Griffith, Ph.D. from Marshall Johnson, Ph.D. ____________ reviewing the Oral Teratology Study of FC-95 in Rats_________________ Oral (Stomach Tube) Developmental Toxicity Study of PFOS in Rabbits, ____________ Study No. 418-012 Argus Labs____________________________________ __________M edical Surveillance and Epidemiology_____________________________________________________ F____________________ inal Reports______________________________________________________________________________ An Epidemiologic Investigation of Clinical Chemistries, Hematology and Hormones in Relation to Serum Levels of Perfluorooctanesulfonate in Male Fluorochemical Production ___________________ Employees______________________________________________ Serum Perfluorooctane Sulfonate and Hepatic and Lipid Clinical ___________________Chemistry Tests in Fluorochemical Production Employees_______ Fluorochemical Exposure (Serum) Assessment of (3M) Decatur ___________________Chemical and Film Plant Employees_________________________ ___________________ Mortality Study of Employees at 3M Plant in Decatur, Alabama Determination of Serum Fluorochemical Levels in Sumitomo 3M ___________________ Employees _________________________________ Analysis of Selected Decatur Employee Serum for Sulfonic and ___________________ Carboxylic Fluorochemicals _____________________________ Page 2 000008 3M Fluorochemical EPA Submissions ____________ Fluorochemical Control Study______________________________ ____________ Working Memorandum on Data Quality Assessment____________ Study Protocols/Proposals o f Ongoing Research__________________________________________ ______ Identification of Fluorochemicals in Sera of Children in the United States Serum fluorochemical trends of out-of-country residents in CLUE I (1974) ______ and Clue II (1989) epidemiologic investigations._____________________ ______ Identification of Fluorochemicals in Human Tissue___________________ An Epidemiologic Analysis of the Inpatient and Outpatient Claims Event ______ and Episode Experience of 3M Decatur Employees, 1993-1998_________ Determination of Serum Elimination half-lives of Ammonium Perfluorooctanoate, Perfluorooctane Sulfonic Acid, Perfluorohexane Sulfonic Acid and Total Organic Fluorine in Decatur Chemical Plant Retirees April 25,2000_____________________________________________________________ ______ Cover Letter to EPA__________________________________________________ Protocol 418-008 Combined oral (gavage) fertility, developmental and perinatal / postnatal reproduction toxicity study of PFOS in rats (Sponsor's No.: 6295.9), Table E20, 1-3_______________________________________________________ Prevention of fluvastatin induced toxicity, mortality, and cardiac myopathy in ______ pregnant rats by mevalonic acid supplementation.__________________________ The mechanism underlying the hyperlipemic effect of perfluorooctanoic acid ______ (PFOA), perfluorooctate sulphonic acid (PFOS) and cclofibric acid.___________ Developmental changes in the expression of genes involved in cholesterol ______ biosynthesis and lipid transport in human and rat fetal and neonatal livers.______ Interoffice memo from Tom Kestner to Leo Gehlhoff, "Fluorochemical Isomer ______ Distribution by ^F-N M R Spectroscopy"_________________________________ April 28 2000 ___________________________ ______ Cover Letter to EPA__________________________________________________ Voluntary Use and Exposure Information Profile - Perfluorooctane Sulfonic Acid and Its Salt Forms __________________________ May 4 200ft ______________________________________________________ ______ Cover Letter to EPA _____________________________________________ ______ 3M TSCA 8(e) Fluorochemical Index of Submissions_______________________ __________Physical/ Chemical Properties_________________________________________________________________ Determination of the Melting Point / Melting Range of PFOS___________ ____________ Boiling Point (Not Conducted)____________________________________ Determination of the Vapor Pressure of PFOS Using the Spinning Rotor ____________ Gauge Method________________________________________________ PFOS: Determination of the n-Octanol / Water Partition Coefficient by the Shake Flask Method - A Non-GLP Feasibility Study in Support of ____________ Wildlife Inti Ltd Project No.: 454C-108_____________________________ Testing Results: Air-Water Partition Coefficient (K ^w ) fr PFOS Page 3 000009 3M Fluorochemical EPA Submissions Determination of the Water Solubility of PFOS by the Shake Flask ______ Method_____ ________________________________________________ ______ Technical Report. Solubility Measurements of FC-95_________________ ______ Solubility Estimate of FC-95 by use of Xertex TOC Analyzer__________ Environmental Fate and Transport________ Adsorption of FC-95 and FC-143 on Soil / Summary of the Soil ______ Adsorption study of the Potassium Salt of Perfluorooctanesulfonic acid ______ Photodegradation (Testing in Progress)_____________________________ FC-95 / Photolysis Study Using Simulated Sunlight / Summary of Photolysis Study Using Simulated Sunlight on the Potassium Salt of ______ Perfluorooctanesulfonic acid__________________________ __________ Biodegradation Studies of Fluorocarbons (8/12/76) report and ______ Biodegradation Studies of Fluorocarbons - III (7/19/78) report._________ BOD / COD results for FC-94-X (Li salt of PFOS)___________________ ______ BOD / COD results for FC-99 (PEA salt of PFOS)___________________ Transport between environmental compartments (fugacity modeling) / letter from Don Mackay on the air / water partitioning coefficient ______ calculations________________________________________ ___________ ______ Analysis for fluorochemicals in Bluegill fish. ___________________ Ecotoxicity Elements__________________________________________________________________ __ PFOS : A 96 -Hour Static Acute Toxicity Test with the Fathead Minnow ______ (Pimephales promelas)__________ ^_____________________________ PFOS : A 96-Hour Toxicity Test with the Freshwater Alga (Selenastrum ______ capricomutum)_______________________________________ _________ PFOS : A 48-Hour Static Acute Toxicity Test with the Cladoceran ______ (Daphnia magna)_______________________________________________ PFOS : A 96-Hour Shell Deposition Test with the Eastern Oyster ______ (Crassostrea virginica)__________________________________________ PFOS : A 96-Hour Static Acute Toxicity Test with the Freshwater Mussel ______ (Unio complamatus)____________________________________________ ______ PFOS : An Activated Sludge, Respiration Inhibition Test______________ PFOS : A 96-Hour Static Acute Toxicity Test with the Saltwater Mysid ______ (Mysidopsis bahia)_____________________________________________ PFOS : An Early Life-Stage Toxicity Test with the Fathead Minnow ______ (Pimephales promelas)__________________________________________ PFOS : A Semi-Static Life-Cycle Toxicity Test with the Cladoceran ______ (Daphnia magna)_______________________________________________ PFOS : A Flow-through Life-Cycle Toxicity Test with the Saltwater ______ Mysid (Mysidopsis bahia)_______________________________________ ______ PFOS : A Dietary LC50 Study with the Mallard_____________________ ______ PFOS : A Dietary LC50 Study with the Northern Bobwhite____________ ______ Multi-Phase Exposure / Recovery Algal Assay Test Method___________ Page 4 OOOOIO 3M Fluorochemical EPA Submissions The Effects of Continuous Aqueous Exposure to 14C-78.02 on Hatchability of Eggs and Growth and Survival of Fry of Fathead Minnow (Pimephales promelas / Summary of histopathological Examinations of ______ Fathead Minnow (Pimephales promelas) Exposed to 78.02 for 30 Days Effect of Potassium Perfluorooctanesulfonate on Survival, etc. (Daphnid ______ reproduction)__________________________________________________ Pimephales promelas 96-hour Toxicity Test Data Summary. Sample FC-94-X (Li salt ofPFOS)_______________________________________ 48-HR Acute Toxicity to Daphnia, Daphnia magna. FC-94-X (Li salt of PFOS)______________________ ________________________________ _ ______ Microbics Microtox Toxicity Test. Sample : FC-94-X (Li salt ofPFOS) Evaluation of FC-94-X by OECD Activated Sludge Respiration Inhibition Test #209, Review of OECD 209 and BOD/COD Test Results for ______ FC-94-X, test data sheets (Li salt ofPFOS)_________________________ 96-Hour Acute Toxicity Test on Bluegill Sunfish (FC-99, DEA salt of PFOS)_________________________________________________ _ ______ Acute Toxicity to Activated Sludge (FC-99, DEA salt ofPFOS)________ Microtox data for FM-3820 (28% PFOS)___________________________ ______ Acute Toxicity to Daphnia magna for FM-3820 (28% PFOS)___________ ______ Toxicity to Algae (Selenastrum capricomutum) for FC-3820 (28% PFOS) Summary Reports________________________________________________________________________ _ _ _ _ _ ______ Final Comprehensive Report: FC-9S_______________________________ Environmental Monitoring - Part 1 : Multi-City Study_________________________________ ______ Design and Structure of Multi-City Study___________________________ ______ Low Level Drinking Water Analytical Method_______________________ Fluorochemical Characterization of Drinking Water Samples. Columbus, GA (W2336)__________________________________________________ Fluorochemical Characterization of Drinking Water Samples. Pensacola, FL (W2176)___________________________________________________ Fluorochemical Characterization of Drinking Water Samples. Port St. Lucie, FL (W2363)_____________________________________________ Fluorochemical Characterization of Drinking Water Samples. Decatur, ______ Alabama (W1979)______________________________________________ Fluorochemical Characterization of Drinking Water Samples. Mobile, ______ Alabama (W2151)______________________________________________ Fluorochemical Characterization of Drinking Water Samples. Cleveland, Tennessee (W1973)_____________________________________________ ______ Draft Drinking Water Health Advisory (DWHA) - PFOS______________ Battelle Field Sampling Procedures Review. Columbus Georgia City Survey regarding Empirical Human Exposure Assessment. Multi-City ______ Study ___________________________________________________ Multi-City Study. Field Report for Cleveland Tennessee and Decatur ______ Alabama - Battelle Duxbury Activities_____________________________ Multi-City Study. Field Report for Columbus Georgia and Port St. Lucie ______ Florida - Battelle Duxbury Activities______________________________ Page 5 O O O O ll 3M Fluorochemical EPA Submissions Final Multi-City Study. Field Report for Mobile Alabama and Pensacola ______ Florida - Battelle Duxbury Activities_______________________________ Quality assurance Project Plan for Empirical Human Exposure ______ Assessment. Multi-City Study Sampling Task_______________________ Amendment 1 to the Quality Assurance Project Plan and Associated SOP's________________________________________________________ Environmental Monitoring - Part 2 : Biosphere Studies________________________________ LCMSMS Analysis of Extracts in: "Preliminary Report Analysis of ______ Perfluorinated Compounds in Environmental Samples"________________ ______ Analysis of Fluorochemicals in Wild Bird Livers_____________________ ______ Screening of PFOS levels in Eagles and Albatross____________________ Perfluorooctane Sulfonate Studies ____________________________________________________ __________ Acute Toxicity_____________________________________________________________________________ An Acute Inhalation Toxicity Study of T-2306 CoC in the Rat, Bio/dynamics, Project No. 78-7185 (FC-95, Perfluorooctane _____________Sulfonate potassium salt)__________________________________ Acute Oral Toxicity (LD50) Study in Rats with Fluorad Fluorochemical Surfactant FC-95, Inti R&D Corp, Project No. _____________ 137-083________________________________________________ Combined Eye and Skin Irritation Studies Report on Sample T- _____________ 1166 (FC-98), Warf Inst, Project No. 5011023.________________ Eye Irritation Study (with washout procedure) Report on Sample _____________T-1166 (FC-98), Warf Inst, Project No. 5011023.______________ __________ Genotoxicity______________________________________ Mutagenicity Evaluation of T-2014 CoC in the Ames Salmonella / Microsome Plate Test Final Report, Litton Bionetics Project No. _____________20838, Protocol No. DMT-100_____________________________ Memo Report from S.R. Rohfing to A.N. Welter, on Results of the Ames Spot Test for Mutagenicity screening of various FCs, including Sample 23-583 which is FC-95, Notebook Reference _____________45867-24, 25.____________________________________________ Mutagenicity Test on T-6295 in an in vivo Mouse Micronucleus Assay, Final Report, Coming Hazleton, CHI Study No. _____________ 17403-0-455, protocol and amended protocol._________________ Final Report, Chromosomal Aberrations in Human Whole Blood Lymphocytes with PFOS, Covance Lab, Covance Study No. _____________20784-0-449, 3M Reference No. T-6295.18 / October 25, 1999. Final Report, Unscheduled DNA Synthesis in Rat Liver Primary Cell Cultures with PFOS, Covance Lab, Covance Study No. _____________ 20784-0-447, 3M Reference No. T-6295.19, and protocol._______ Final Report, Salmonella Escherichia coli / Mammalian- Microsome Reverse Mutation Assay with PFOS, Covance Lab, Covance Study No. 20784-0-409, Reference No. T-6295.17, and _____________ protocol.________________________________________________ Page 6 O O O O ifa s 3M Fluorochemical EPA Submissions Final Report, In Vitro Microbiological Mutagenicity Assays of 3M Company Compounds T-2247 CoC and T-2248 CoC, SRI Inti, SRI Project No. LSC-4442-016, 3M Reference No. T-2247.1 (FC-99 Old Formula, L-4299 which is 50% of the diethanolamine salt of ______ perfluorooctanesulfonate in water)___________________________ Evaluation of Mutagenicity Studies Developed on (PFOS) ______ Perfluorooctane Sulfonate__________________________________ Final Report - Bacterial Reverse Mutation Assay of x-1, Hita Research Lab / Study Code K01-1802, 3M Reference No. T______ 6667.1 (FC-98, Potassium Perfluoroethylcyclohexyl Sulfonate) Repeated-Dose Toxicity_________________________________________________________________ Ninety-Day Subacute Rhesus Monkey Toxicity Study, with Fluorad Fluorochemical Surfactant FC-95, Inti Research and ______ Development Corp, Project No. 137-092_____ Aborted Study : Ninety-Day Subacute Rhesus Monkey Toxicity Study, with Fluorad Fluorochemical Surfactant FC-95, Inti R&D Corp, Project No. 137-087___________________ Ninety-Day Subacute Rat Toxicity Study, with Fluorad Fluorochemical Surfactant FC-95, Inti R&D Corp, Project No. 137-085_______________________________________________ _ 104-week Dietary Chronic Study and Carcinogenicity Study with Perfluorooctane Sulfonic Acid Potassium Salt (PFOS: T-6295) in ______ Rats, Covance Lab, Study No. 6329-183.______________________ ____________ Summary Report - Week 53 undated___________________ "Liver Slide Review", Marvin Case to John Butenhoff and Andrew Seacat relaying the results of an independent ____________ histopathologic review of liver slides from the study.______ Second Draft Cell Proliferation Report, Pathology ____________ Associates Inti._____________________________________ Study Report of Determination of Cyanide Insensitive Palmitoyl-CoA oxidation in samples from 3M Environmental Lab - Covance Studies 6329-183 and 6329____________ 212, Study No. XRO108_____________________________ Range-finder: 4-Week Capsule Toxicity Study with Perfluorooctane Sulfonic Acid Potassium Salt (PFOS; T-6295) in ______ Cynomolgus Monkeys, Covance Labs, Study No. 6329-222_______ Unaudited Draft Final Report, 4-Week Capsule Toxicity Study with Perfluorooctane Sulfonic Acid Potassium Salt (PFOS; T-6295) in Cynomolgus Monkeys, Covance Labs, ____________ Study No. 6329-222 (draft not complete)._______________ Cell Proliferation Report, 4-Week Capsule Toxicity Study with Perfluorooctane Sulfonic Acid Potassium Salt (PFOS; T-6295) in Cynomolgus Monkeys, Covance Labs, Study ____________ No. 6329-222 (draft to be incorporated in Fmal report) Page 7 000012 3M Fluorochemical EPA Submissions Protocol - Analytical Study, Quantitative Analysis of Perfluorooctane Sulfonic Acid Potassium Salt (PFOS; T- 6295) in Cynomolgus Monkeys Following Administration of a 4-Week Capsule Toxicity Study, 3M Environmental ____________ Lab, AMDT-041598.1_______________________________ Memo from Marvin Case, re: histopathology ____________ review of liver tissue in Covance Study 6329-222________ 26-Week Capsule Toxicity Study with Perfluorooctane Sulfonic Acid Potassium Salt (PFOS: T-6295) in Cynomolgus Monkeys, ______ Covance Labs, Study No. 6329-223. In Progress_______________ Undated report covering the 26-week dosing phase and one ____________ year recovery.______________________________________ Dose-Setting Rationale for Six-Month Chronic Oral Study ____________ in Cynomolgus Monkeys____________________________ ____________ Fecal Urbilinogen Analysis, Mayo Clinic, Test Code: 8308. __________________ Summary Report from Dr. Joseph P. McConnell General Info from Mayo Clinic on Urobilinogen __________________ Analysis____________________________________ Individual animal urobilinogen lab reports from __________________ Mayo Clinic________________________________ Pathology Report, Electron Microscopic Evaluation of Liver in Cynomolgus'Monkeys, Pathology Associates Inti, ____________ Study No. EM99.76________________________________ Pathology Review, Marv Case to Andrew Seacat, relaying ____________ the results of a histopathology review of slides.__________ Lab Report, Interim Report of Preliminary Data for 26 Week Capsule Toxicology Study with PFOS in Cynomolgus Monkeys, 3M Environmental Lab, Report No. ____________ FACT-TQX-030___________________________________ Two Week Oral Rangefinding Toxicity Study of T- 2509CoC in Rats, Safety Evaluation Lab, Riker Labs, Experiment No. 179RR023, 3M Reference No. T-2509.3 (FC-99 New Formula, L-4509, 25% diethanolamine salt of ____________ perfluorooctanesulfonate in water)_____________________ Pharmacokinetic Studies_______________________________________________________________ Skin Absorption Studies on Surfactants Report from W.C. ______ McCormick to D.R. Ricker_________________________________ Skin Absorption Studies on Surfactants 28 Day Percutaneous Absorption Study in Rabbits with FC- 95, Safety Evaluation Lab, Riker Labs, Experiment No. 0979AB0632 (FC-95)________________ Skin Absorption Studies on Surfactants 28 Day Percutaneous Absorption Study in Rabbits with FC-99, Safety Evaluation Lab, Riker Labs, Experiment No. 0979AB0633, 3M Reference No. T-3988.1 ______ ______ Page 8 000013 3M Fluorochemical EPA Submissions Single-Dose Intravenous Pharmacokinetic Study of T-6053 in Rabbits, 3M Environmental Lab. Final Report - Analytical Study, Hazleton Wisconsin Project No. HWI 6329-136, 3M Reference No. T-6053.1_____________________________________________ Single-Dose Dermal Absorption / Toxicity Study of T-6053 in Rabbits, 3M Environmental Lab, Study No. AMDT-022195.1. Final Report - Analytical Study, Hazleton Wisconsin, Project No. HWI 6329-137, 3M Reference No. T-6053.2___________________ Fluorochemical (FC) Levels in Naive Rats, 3M Medical Department, Toxicology Services, Study No. T-6316.9, DT21, Draft Report for Objective 3________________________________ Analytical Data submitted to Dr. Jennifer Seed, USEPA, by letter dated May 3, 2000, including serum measurements from two in life studies_______________________________________________ Analytical data from Advanced Bioanalytical Services Study No. FACT-TOX-111, re: Oral (Gavage) Pharmacokinetic Recovery Study of Perfluorooctane Sulfonate in Rats, Argus Labs Protocol No. 418-015, 3M ______ Reference T-6295.14._______________________________ Analytical data from Advanced Bioanalytical Services Study No. FACT-TOX-110., re: Oral (Gavage) Pharmacokinetic Study of Perfluorooctane Sulfonate in Rats, Argus Labs Protocol No. 418-013, 3M Reference T- 6295.12.__________________________________________ In Vitro Comparative Metabolism Study in Rat and Human Hepatocytes with Various Fluorochemicals, 3M Reference T- 6295.1, study o f T-6292, T-6293, T-6294, and T-6295.___________ Range-finding Cytotoxicity Assay, SRI Inti Toxicology ______ Lab, Study No. BO10-95 .____________________________ Metabolism of T-6292, T-6293, T-6294, T-6295 by Rat and Human Hepatocytes, SRI Inti Toxicology Lab, Study No. BO11-95__________________________________________ Advanced Bioanalytical Services, Analytical Report, Additional Characterization of Metabolites of T-6292, T- 6293 and T-6294 from Rat and Human Hepatocytes by TurboIonSpray LC/MS and LC/MS/MS. Semi- Quantitative Analysis of T-6295 in Rat and Human Hepatocytes Incubated with T-6292, T-6293 and T-6294 by LC/MS/MS, Report 98AGKP01.3M___________________ Working Interpretation of Results, chart titled Perfluorosulfonamide Metabolism in Rat vs. Human ______ Hepatocytes. _________________________________ Page 9 000014 3M Fluorochemical EPA Submissions _________ Mechanistic _________________________________________________________________ One Generation Reproduction Study of PFOS - Mevalonic Acid / Cholesterol Challenge and NOEL Investigation in Rats. Draft ____________ Study Outline___________________________________________ One Generation Reproduction Study of PFOS in Rats - ____________ Pharmacokinetic Analysis. Study Outline___________________ One Generation Reproduction Study of PFOS in Rats - ____________ Pharmacokinetic Analysis. Study Outline____________________ Biochemical and Molecular Mechanistic Studies of N-Alkyl ____________ Perfluorosulfonamides, Research Proposal, Kendall B. Wallace The Effect of Perfluorinated Arylalkylsulfonamides on Bioenergetics of Rat Liver Mitochondria, Kendall B. Wallace and ____________ Anatoli Starkov_________________________________________ Report on Covance Studies, assessment of mitochondrial ____________ bioenergetics___________________________________________ Summary of the Effect of PFC's on Mitochondrial Bioenergetics ____________ In Vitro________________________________________________ Effects of Selected Perfluoro-compounds on Mitochondrial Beta- ____________ Oxidation______________________________________________ Effect of Acute FC Administration on Catalase and acylCoA ____________ Oxidase Expression_______________________________ ______ Displacement of a Fluorescetitly Labeled Fatty Acid Analogue from Fatty Acid Carrier Proteins by Wyeth - 14,643, Ammonium Perfluorooctanotate, Potassium Perfluorooctane Sulfonate and ____________ Other Known Peroxisome Proliferators, Nabbefeld, et al._______ __________ Teratology_________________________________________________________________________________ Pilot Teratology Study in Rats, T-3551 Final Report, 3M ____________ Reference T-3551.12_____________________________________ _________ Human Sera /M edical Surveillance/Epidemiology__________________________ Memo from D.E. Roach and S.D. Sorenson, Decatur Blood ____________ Fluoride Review________________________________________ ____________ Antwerp Blood Testing Results____________________________ ____________ Analysis of Serum Values in Decatur Workers________________ Lab Report, Analysis of FCs in Samples of Children's Sera, Lab ____________ Report No. FACT-GEN-011, 3M Environmental Lab__________ Lab Composite Report, Analytical Reports of Data for Fluorochemical Analysis in Human Sera, LIMS No. 1623, 3M ____________ Environmental Lab______________________________________ Ongoing Environmental Studies On Perfluorooctanesulfonates____________________ __________Environmental Fate and Transport_______________________________________________ Abiotic Degradation Studies. Includes Hydrolysis and Photolysis ____________ Study Plans_____ ______________________________________ Biodegradation Studies (Includes Studies on : aerobic acclimated closed bottle biodegradation, aerobic soil / sediment biodegradation, pure culture aerobic, and fluorochemical ____________ decomposition process) ______________________________ Page 10 000015 EPA Submission Draft __________Ecotoxicity Elements____________________________________________________________________ Protocol: PFOS: A 96-Hour Toxicity Test with the Freshwater ____________ Alga___________________________________________________ Protocol: PFOS: A 96-Hour Toxicity Test with the Freshwater ____________ Diatom_________________________________________________ ____________ Protocol: PFOS: A 96-Hour Toxicity Test with the Marine Diatom ____________ Protocol: PFOS: A 7-Day Toxicity Test with Duckweed_________ Perfluorooctane Sulfonate Studies_______________________________________________________ __ __________ Ongoing Research /S tu d y Protocols_______________________________________________ _ Protocol for Study : Low Level PFOS Dose versus Rat Serum and Liver PFOS, 3M Medical Department, Toxicology, Study No. ____________ T-6295.16 DT31_________________________________________ Protocol for Study : Pharmacokinetic Study of POSF in Rats, 3M ____________ Medical Department, Toxicology, Protocol for Study No. T-7098.1 Pre-1976 Environmental Studies On Perfluorooctanesulfonates_____________________ Data from Fathead Minnow Study on FC-93 (25% NH4 salt of PFOS in ______ IPA and water), 3M Environmental Lab, Aug. 2, 1974.________________ Data from Fathead Minnow Study on FC-93 (25% NH4 salt of PFOS in ______ IPA and water), 3M Environmental Lab, Oct. 19, 1974,________________ BOD / COD results for FC-93 (25% NH4 salt of PFOS in IPA and water), ______ 3M Environmental Lab, July 18, 1974______________________________ May 11,2000____________________________________________________________ Cover Letter to EPA / additional information relating to perfluorooctylsulfonate (PFOS) salts_______________________________________________________ May 12,2000________________________________________________ ______ Cover Letter to EPA from Julia Hatcher (Latham & Watkins) Composite Analytical Lab Report on the Quantitative Analysis of ______ Fluorochemicals in Environmental Samples__________________ May 15,2000_____________________________________________________________ ______ Cover Letter to EPA__________________________________________________ ______ Summaries of the environmental science areas____________________________ Summary "26-Week Capsule Toxicity Study with Perfluorooctanesulfonic Acid Potassium Salt (PFOS) in Cynomolgus Monkeys, Current Summary as of May 4, 2000 ____________________________________________________________________ Cover Letter to the resubmittal of confidential information previously submitted ______ on May 4.________ _ _ _ _ _ _ _________________________________________ Mav 18,2000 __________ ______ Cover Letter to EPA__________ ______ N-EtFOSE Alcohol Bibliography __________Physical / Chemical Properties ____________ Water Solubility_______ Page 11 000016 3M Fluorochemical EPA Submissions ______ Letter Report. Boiling Point Information___________________________ Determination of Vapor Pressure Curve by Dynamic Method for U1463 (EtFOSE)____________________;_______________________ _ ______ Memo - Melting Point of FM-3923 N-Ethyl FOSE Alcohol____________ Environmental Fate and Transport______________________________________ Tech Report - Biodegradation Studies of Fluorocarbons, Request for Lab ______ work - BOD/COD, Memo - Tech Report_____ Tech Report - Bioconcentration of FM3422 in Bluegill Sunfish and in Channel Catfish. Tech Report - Aquatic Fate of a Fluorochemical : FM3422___________________________________________________ ___ ______ Tech Notebook copies - Analytical from fish bioconcentration studies ______ Alkaline hydrolysis_____________________________________________ ______ Pilot studies on soil adsorption___________________________________ ______ Evaluation of the Bioconcentration Potential of FM-3422______________ ______ Tech Report - Adsorption of FM-3422 on Soil_______________________ ______ Tech Report - Analysis for Fluorochemicals in Bluegill Fish___________ Tech Report - Bioaccumulation of Fluorochemicals in Term. River. Tech ______ Report - Fluorochemicals in Tennessee River Fish.___________________ ______ Tech Report - Photolysis of FM-3422 on Soil_____________________ __ ______ Tech Report - FM-3422 Photolysis Study using Simulated Sunlight_____ Summary Reports________________________________ _________________________________________ ___ ______ Tech Report - Analytical Methodology on FM-3422__________________ ______ Tech Report - Analytical Methodology and Support__________________ Ecotoxicity Elements______________________________________________________________________________ The Effects of Continuous Aqueous Exposure to 78.01 on Hatchability of Eggs and Growth and Survival of Fry of Fathead Minnow. Summary of Histopathological Examinations of Fathead Minnow Exposed to 78.01 for ______ 30 Days.______________________________________________________ ______ Tech Report - Multi-Phase Algal Assay Test Method._________________ N-MeFOSE Alcohol______________________________________________________________________________ Physical / Chemical Properties_________________________________________________________________ ______ Tech Report - Solubility of FM-3925______________________________ Environmental Fate and Transport__________________________________________________________ Tech Report - Biodegradation Studies on FM-3925. Tech Report ______ Chemical Oxygen Demand of FM-3925____________________________ ______ Tech Report - Distribution coefficient of FM-3925 in n-Octanol / Water ______ Tech Report - Adsorption of FM-3925 on Soil______________________ Tech Report - Bioaccumulation of Fluorochemicals in Term. River Fish, Tech Report - Fluorochemicals in Tenn. River Fish, Ar No. 7238 ______ Determination of Fluorinated Alcohols in Fish Extracts_______________ Ecotoxicity Elements______________________________________________________________________________ ______ Tech Report - Aquatic Toxicity Studies : FM-3925___________________ N-Ethylperfluorooctanesulfonamide = Ethyl Amide Physical / Chemical Properties_________________________________________________________________ ______ Determination of Vapor Pressure curve by dynamic Method for U1464 Page 12 000017 3M Fluorochemical EPA Submissions (Et-FOSA)_____________________________________________________ ______ Letter Report. Boiling Point Information____________________________ Environmental Fate and Transport____________________________________________________________ Tech Report - Bioaccumulation of Fluorochemicals in Term. River Fish. ______ Tech Report - Fluorochemicals in Term. River Fish___________________ ______ Environmental Lab Final Report for BOD / COD_____________________ Ecotoxicity Elements_______________________________________________________________________________ ______ Fathead minnow static acute toxicity test - FX-12_____________________ ______ Daphnia magna static acute toxicity test - FX-12______________________ ______ Acute Toxicity of U 1464 to Ceriodaphnia dubia______________________ ______ Acute Toxicity of U 1464 to Larval Fathead Minnow___________________ ______ Inhibition of U 1464 for Activated Sludge Respiration Inhibition_________ ______ Acute Toxicity of U 1464 to Daphnia magna_________________________ P O S F ___________________________________________________________________________________________________ Physical / Chemical Properties__________________________________________________________________ ______ Water solubility estimate (screen)__________________________________ Ecotoxicity Elements_______________________________________________________________________________ ______ 96-hour Toxicity to Fathead Minnow_______________________________ 7-Minute Exposure Activated Sludge Toxicity Test Perfluorooctane Sulfonate-Related Compounds___________________________________________ POSF________________________________________________ ;_________________________________________________ _ Acute Toxicity________________________________________________________________________________________ Primary Skin Irritation Test with T-3874 in Albino Rabbits, Pathology and Toxicology, Riker Labs, Project No. 0386EB0135, 3M Reference No. T-3874________________________________________________________ T-3607 Acute Inhalation Toxicity Test, Bushy Run Research Center, ______ Project No. 47-527, 3M Reference No. T-3607_______________________ Acute Oral Toxicity Study - Method, Summary, Pathology and Raw Data ______ Appendix, Hazleton Labs, Project No. 40703983______________________ Genotoxicity _________________________________________________________________________________ In Vitro Microbiological Assays of 3M Company Compounds T-2540 CoC and T-2541 CoC, SRI Inti, Project No. LSC 4442-16, 3M Reference No. T-2541.1___________________________________________________ Ongoing Research /S tu d y Protocols__________________________________________________________ Protocol, Pharmacokinetic Study of POSF in Rats, 3M Strategic Toxicology Lab, 3M Reference No. T-7098.1________________________ FOSA ________________________________________________________________________________ Acute Toxicology________ __________________________________________________________________________ Acute Oral Toxicity Screen with T-3421 in Albino Rats, Safety Evaluation ______ Lab, Riker Labs, Project No. 0883AR0287, 3M Reference No. T-3421 Acute Ocular Irritation Test with T-3421 in Albino Rats, Safety Evaluation Lab, Riker Labs, Project No. 0883EB286, 3M Reference No. T-3421 _________________________ Page 13 000018 3M Fluorochemical EPA Submissions Primary Skin Irritation Test with T-3421 in Albino Rabbits, Safety Evaluation Lab, Riker Labs, Project No. 0883AR0288, 3M Reference No. T-3421_____________________________________________________ __ Studies in Progress________ _____________________________________________________________________ ___ Protocol, Feces Method Development Metabolism Study for Perfluorooctanesulfonate Derivatives, 3M Strategic Toxicology Lab, Study Nos. T-636.17; T-629S.21; T-7132.3; ST-41____________________ Protocol, Pharmacokinetic Study of Perfluorooctane Sulfonamide in Rats, ______ 3M Strategic Toxicology Lab, Study Nos. T 132.2; ST-39______________ Protocol, Cell Proliferation Study with N-Ethyl Perfluorooctanesulfonamido Ethanol, Perfluorooctane Sulfonic Acid Potassium Salt, and N-Ethyl Perfluorooctanesulfonamide in Rats, ______ Pathology Associates Inti, Study No. 1132-100_______________________ PFOSAA______________________________________________________________________________________________ Acute Toxicity_____________________________________________________________________________________ __ Acute Oral Toxicity - Rats, Biosearch, Project No. 77-1108A, 3M ______ Reference No. T-1983 (FC-128)___________________________________ Acute Oral Toxicity - Rats, Biosearch, Project No. 77-1127A, 3M Reference No. T-2001 (FC-128)___________________________________ Primary Eye Irritation Study - Rabbits, Biosearch, Project No. 77-1127A, 3M Reference No. T-2001 (FC-128)________________________________ Primary Skin Irritation Study - Rabiuts, Biosearch, Project No. 77-1127A, 3M Reference No. T-2001 (FC-128)________________________________ An Acute Inhalation Toxicity Study of T-2307 CoC in the Rat, ______ Bio/dynamics, Project No. 78-7186, 3M Reference No. T-2307 (FC-128) Genotoxicity__________________________________________________________________________________________ Bacterial Reverse Mutation Test of v-1, Hita Research lab, Chemicals Inspection and Testing Institute, Study Code: KOl-1815, Report No. T-4663, 3M Reference No. T-6668.1, FC-129________________________ In Vitro Microbiological Mutagenicity Assays of 3M Company's Compound T-3290 CoC, SRI Inti, Project No. 3145, 3M Reference No. T-3290________________________________________________________ Pharmacokinetic Studies_________________________________________________________________________ 28 Dermal Percutaneous Absorption Study with FC-128 in Albino Rabbits, Safety Evaluation Lab, Riker Labs, Project No. 0979AB0629, 3M ______ Reference No. T-3991___________________________________________ 28 Day Dermal Percutaneous Absorption Study with FC-129 in Albino Rabbits, Safety Evaluation Lab, Riker Labs, Project No. 0979AB0627, 3M ______ Reference No. T-3989___________________________________________ Final Report - Analytical Study: Single-Dose Dermal Absorption / Toxicity Study of T-6051 and T-6054 in Rabbits, 3M Environmental Lab, Study No. ADMT-013195.1, in vivo Study Reference No. HWI 6329-133, 3M Reference Nos. T-6051 and T-6054 Page 14 000019 3M Fluorochemical EPA Submissions Final Report, Analytical Report and Single-Dose Intravenous Pharmacokinetics Study of T-6054 in Rabbits, 3M Environmental Technology & Services, In-Vivo Study Reference No. HWI#6329-138, Study No. AMDT-122094.2, 3M Reference No. FC-129_______________ Studies in Progress_________________________________________________________________________________ Corporate Toxicology Study Outline, FC-129 Preliminary ADME Screen ______ in Rats, 3M Strategic Toxicology Lab ____________________________ PFDS___________________________________________________________________________________________________ Acute Toxicity_______________________________________________________________________________________ Final Report, Acute Dermal Toxicity Study in Rabbits, Hazelton Labs, 3M ______ Reference No. T-4102, Samples No. T837389-410 754________________ Final Report, Acute Oral Toxicity Study in Rats, Hazelton Labs, 3M ______ Reference No. T-4102, Sample No. T837389-410 754_________________ Final Report, Primary Eye Irritation / Corrosion Study in Rabbits, ______ Hazelton Labs, 3M Reference No. T-4102, Sample No. T837389-410 754 Final Report, Primary Dermal Irritation / Corrosion Study in Rabbits, Hazelton Labs, 3M Reference No. T-4102, Sample No. T837389-410 754 Genotoxicity__________________________________________________________________________________________ Mutagenicity Test with T-6357 in the Salmonella - Escherichia coli / Mammalian-Microsome Reverse Mutation Assay, Coming Hazleton, ______ Project No. 17387-0-409, 3M Reference No. T-6357, FC-120___________ Mutagenicity Test on T-6357 in an In Vivo Mouse Micronucleus Assay, Coming Hazleton, Project No. 17387-0-409, 3M Reference No. T-6357, FC-120_______________________________________________________ Pharmacokinetic Studies_________________________________________________________________________ Final Report, Analytical Report and Single-Dose Dermal Absorption / Toxicity Study of T-6052 in Rabbits, Hazleton, Project No. HWI 6329- 135, 3M Reference No. FC-120, T-6052____________________________ Single-Dose Dermal Intravenous Pharmacokinetic Study of T-6052 in ______ Rabbits, Hazleton, Project No. HWI 6329-134, 3M Reference No. T-6052 N-EtFOSA____________________________________________________________________________________________ Acute Toxicity_______________________________________________________________________________________ Final Report, Acute Ocular Irritation Test with T-3608 in Albino Rats, ______ Riker Labs, 3M Reference FX-12, Study No. 0984EB0367_____________ Final Report, Primary Skin Irritation Test with T-3608 in Albino Rats, Riker Labs, 3M Reference FX-12, Study No. 0984EB0368_____________ Final Report, Acute Oral Toxicity Screen with T-3066 CoC in Albino ______ Rats, Riker Labs, 3M Reference No. FX-12, Study No. 0981AR0146 Genotoxicity _________________________________________________________________________ Final Report, Protocol and two amendments, Mutagenicity Test on T-6294 in an In Vivo Mouse Micronucleus Assay, Coming Hazelton, Study No. 1785-0-455 __________________________ Page 15 000020 EPA Submission Draft Mechanistic___________________________________________________________________________________________ T.J. Cross and R.G. Schnellmann, Mechanism of Toxicity of a Unique Pesticide N-Ethylperfluorooctane Sulfanomide (NEPFOS), and its metabolite perfluorooctane Sulfonamide (PFOS) to Isolated Rabbit Renal Cortical Mitochondria, Abstract from 1989 Society of Toxicology ______ Meeting_______________________________________________________ Analytical___________________________________________________________ Analytical and Research Properties - 3M Industrial Hygiene Lab N-MeFOSA___________________________________________________________________________________________ Acute Toxicity____________________________________________________________________________________ ___ Acute Oral Toxicity - Method, Summary, Pathology QAU Report, ______ Hazleton Labs, Project No. 50503499, 3M Reference No. T-3752________ Primary Dermal Irritation - Method, Summary, Pathology QAU Report, ______ Hazleton Labs, Project No. 50503500, 3M Reference No. T-3752________ Primary Eye Irritation - Method, Summary, Pathology QAU Report, ______ Hazleton Labs, Project No. 50503501, 3M Reference No. T-3752________ Acute Oral Toxicity - Method, Summary, Pathology; Primary Dermal Irritation - Method, Summary; Primary Eye Irritation - Method, Summary; QAU Report; Raw Data Appendix, Hazleton Labs. Project No. ______ 50202473, 3M Reference No. T-3727_______________________________ Acute Oral Toxicity Screen with T-3065CoC in Albino Rats, Riker Labs, ______ Experiment No. 0981AR0145_____________________________________ Genotoxicity_______________________________________ ^__________________________________________________ In Vitro Microbiological Mutagenicity Assays of T-3752, SRI Inti, Project No. LSC-3145, 3M Reference No. T-3752___________________________ In Vitro Microbiological Mutagenicity Assays of T-3727, SRI Inti, Project No. LSC-3145, 3M Reference No. T-3727___________________________ N-EtFOSE Alcohol________________________________________________________________________________ Acute Toxicity_______________________________________________________________________________________ Corrected Final Report, Acute Inhalation Toxicity Study in Rats, 3M ______ Reference No. T-29991IT, Hazelton Labs, Study No. 154-157___________ Genotoxicity__________________________________________________________________________________________ Final Report, Mutagenicity Test on T-5710 in an In Vivo Rat ______ Micronucleus Assay, Hazelton, Study No. 15516-0-454________________ Final Report, Genotoxicity Test on T-5710.1 in the In Vivo / In Vitro Unscheduled DNA Synthesis and Cell Proliferation in Rat Liver Cells, ______ Hazelton, Study No. 15516-0-494__________________________________ Final Report, Mutagenicity Test on T-6292 in an In Vivo Mouse ______ Micronucleus Assay, Hazelton, Study No. 17384-0-455________________ Evaluation of the Mutagenic Activity of T-6906 in an In Vitro Mammalian Cell Gene Mutation Test with L5178Y Mouse Lymphoma Cells, NOTOX ______ Study No. 223458, 3M Reference No. FC-3923 Final Report___________ Letter from Steve R. Haworth, Covance Labs, reviewing the NOTOX ______ study and concluding it was technically inadequate and should be repeated Repeated-Dose Toxicity ______________________________________________________________ Final Report, Ninety Day Subacute Rat Toxicity Study on FM-3422, Inti ______ R&D Corp, Study No. 137-086____________________________________ Page 16 000021 3M Fluorochemical EPA Submissions Final Report, Ninety Day Subacute Rhesus Monkey Toxicity Study, on FM-3422, Inti R&D Corp, Study No. 137-088_______________________ Report, Volumes 1-5, Two Year Oral (Diet) Toxicity / Carcinogenicity Study of Fluorochemical FC-3924 in Rats, Riker Labs, Study No. 0281 CROP12________ _________________________________________ Report Amendment No. 1, Two Year Oral (Diet) Toxicity / Carcinogenicity Study of Fluorochemical FM-3924 in Rats, Riker Labs, Study No. 0281 CROP 12_________________________________________ Xenos Letter dated May 24, 1991 with attachment: 3M Response to FDA Letter of December 10, 1990 [re: 2 year cancer study], Food Additive ______ Petition Nos. 0B4197 and 0B4206_________________________________ Pathology Review of Reported Tumorigenesis in a Two Year Study of ______ FM-3924 in Rats, Pathology Associates Inti_________________________ ______ Analytical on retained sample____________________________________ Review by Dr. William H. Butler of Two Year (Diet) Toxicity / ______ Carcinogenicity Study of Fluorochemcial FM-3924 in Rats____________ Summary Report of Week 53, 104-Week Dietary Carcinogenicity Study with Narrow Range N-Ethyl Perfluorooctanesulfanoamido Ethanol in ______ Rats, 3M Reference No. T-6316.1, Covance Labs, 6329-212 and -228 Pharmacokinetic Studies________________________________________________________________________ ______ Synthesis and Characterization of N-Ethyl FOSE *4C, Riker Labs______ Final Report, Absorption and Biotransformation of N-Ethyl FOSE and Tissue Distribution and Elimination of Carbon-14 after Administration of ______ N-Ethyl FO SE-^C in Feed, Riker Labs____________________________ Mechanistic_________________________________________________________________________________________ Final Report, Analysis of T-5877 in a Cell Proliferation Assay in Rat Liver Cells, Hazelton, Study No. 154-208, 3M Reference T-5877, FM- 3924, Lot 547, L-13202, Protocol, Key to FC Alcohol Tox Samples, ______ Analytical data__________________________ _____________________ Teratology___________________________________________________________________________________________ Final Report, Oral (Gavage) Developmental Toxicity Study of N-EtFOSE ______ in Rats, 3M Reference No. T-6316.7_______________________________ Summary N-EtFOSE Rat Teratology, Oral (Gavage) Developmental ______ Toxicity Study of N-EtFOSE in Rats, 3M Reference No. T-6316.7______ Final Report, Oral (Stomach Tube) Developmental Toxicity Study of N-EtFOSE in Rabbits, 3M Reference No. T-6316.8___________________ Summary N-EtFOSE Rabbit Teratology, Oral (Stomach Tube) Developmental Toxicity Study of N-EtFOSE in Rabbits, 3M Reference No. T-6316.8__________________________________________________ Final Report, Oral Rangefinder Study of T-2999CoC in Pregnant Rabbits, ______ Safety Evaluation Lab, Riker Labs, Study No. 0680RB0019____________ Final Report and Protocol, Oral Teratology Study of T-2999CoC in Rabbits, Safety Evaluation Lab, Riker Labs, Study No. 0681TB0212, 3M Reference No. FM-3924 ________________ Page 17 000022 3M Fluorochemical EPA Submissions 3M Internal Correspondence re alcohols being used in Riker studies, from ______ D.R. Ricker and W.C. McCormick________________________________ Analytical Analyses of Suspension of FM-3924, Internal Memo, from ______ T.R. Mathisen to E.G. Gortner_________________________________ _ ______ 3M Internal Correspondence, Analytical Evaluation of FM-3924________ Final Report, Oral Teratology Study of FM-3422 in Rats, Safety ______ Evaluation Lab, Riker Labs, Study No. 0680TR0010 _____________ 3M Internal Correspondence re alcohols being used in Riker studies, from ______ D.R. Ricker to W.C. McCormick__________________________ _______ Final Report, Special Lens Oral Teratology Study of T-2999CoC in Rats, Safety Evaluation Lab, Riker Labs, Study No. 0680TR0020, 3M ______ Reference No. FC-3924_________________________________________ Final Report and Protocol, Special Lens Oral Teratology Study of T2999CoC in Two Strains of Rats, Safety Evaluation Lab, Riker Labs, Study No. Q681TR0362_____________________________________ __ 3M Internal Correspondence re alcohols being used in Riker studies, from ______ D.R. Ricker to W.C McCormick_______________________________ __ Analytical Analyses of Suspension of FM-3924, Internal Memo, from ______ T.R. Mathisen to E.G. Gortner____________________________ _ 3M Internal Correspondence re analytical analysis of FM-3924, from E.G. ______ Gortner to R.M Payfer_______________________________ __________ Memo from E.G. Lambrecht to Riker Study Files re Fetal Lens Artifact ______ Summary of Developments to Date________________________________ Studies In Progress______________________________________________________________________ ___ Protocol, N-EtFOSE Bile Method Development in Rats, 3M Strategic ______ Toxicology Lab, Study No. T-6316.15; ST-30_______________________ Protocol, Feces Method Development Metabolism Study for Perfluorooctanesulfonate Derivatives, 3M Strategic Toxicology Lab, Study Nos., T-636.17; T-6295.21; T-7132.3; ST-41___________________ Protocol, Cell Proliferation Study with N-Ethyl Perfluorooctanesulfonamido Ethanol, Perfluorooctane Sulfonic Acid Potassium Salt, and N-Ethyl Perfluorooctanesulfonamide in Rats, ______ Pathology Associates Inti, Study No. 1132-100______________________ N-MeFOSE alcohol_______________________________________________________________________________ A cute Toxicity______________________________________________________________________________________ Acute Oral Toxicity - Rats, Biosearch, Inc, 3M Ref. T-2038CoC, Study No. 78-1161A_________________________________________________ Primary Eye Irritation Study - Rabbits, Biosearch, Inc., 3M Ref. T-2038CoC, Study No. 78-1161A_________________________________ Primary Skin Irritation Study - Rabbits, Biosearch, Inc., 3M Ref. T2038CoC, Study No. 78-1161A___________________________________ Acute Oral Toxicity Screen with T-2574CoC in Albino Rats, Riker Labs, ______ Experiment 0979AR0037________________________________________ Page 18 000023 3M Fluorochemical EPA Submissions Genotoxicity________________________________________________________________________________________ Genotoxicity Test on T-5711.1 in the In Vivo / In Vitro Unscheduled DNA Synthesis and Cell Proliferation Assays in Rat Liver Cells, Hazelton, HWA Study No. 15515-0-494_____________________________________ Mutagenicity Test on T-5711 in an In Vivo Rat Micronucleus Assay, ______ Hazelton, HWA Study No. 15515-0-454_______ ______________ _ Evaluation of the Mutagenic Activity of T-5874 in the Ames Salmonella / Microsome Test, NoTox Project 115932, NoTox Substance 38187, 3M ______ Reference T-5874.1___________________________ _ Evaluation of the Ability of T-5874 to Induce Chromosome Aberrations in Cultured Peripheral Human Lymphocytes, NoTox Project 115919, NoTox ______ Substance 38187, 3M Reference T-5874.2_____________________ _ Evaluation of the Mutagenic Activity of T-5874 in an In Vitro Mammalian Cell Gene Mutation Test with L5178Y Mouse Lymphoma Cells, NoTox ______ Project 115921, NoTox Substance 38187, 3M Reference T-5874.3_______ Repeated-Dose Toxicity___________________________________________________________________________ 13-Week Dietary Toxicity Study with N-Methyl Perfluorooctanesulfonamide Ethanol (N-MeFOSE, T-6314) in Rats, Covance Study No. 6329-225, 3M Reference No. T6314.1 Audited Draft ______ Report_____________________________________________________ Letter from Andrew M. Seacat to Peter J. Thomford, Study Director at ______ Covance, re errors in draft report. 13-Week Dietary Toxicity Study______ Mechanistic__________________________________________________________________________________________ Analysis of T-5794 in a Cell Proliferation Assay in Rat Liver Cells, Hazelton, Final Report, HWA Study No. 154-207, and protocol and ______ analytical data__________________________________________________ Analysis of T-5878 in a Cell Proliferation Assay in Rat Liver Cells, Hazelton, Final Report, HWA Study No. 154-209, and protocol and analytical data : N-EtFOSEA Acute Toxicity_______________________________________________________________________________________ Acute Oral Toxicity Screen with T-3493 in Albino Rats, Safety Evaluation ______ Lab, Riker Labs, Project No. 0884AR0010, 3M Reference No. T-3943 Primary Skin Irritation Test with T-3493 in Albino Rats, Safety Evaluation Lab, Riker Labs, Project No. 0884EB0009, 3M Reference No. T-3943________________________________________________________ Acute Ocular Irritation Test with T-3493 in Albino Rabbits, Safety Evaluation Lab, Riker Labs, Project No. 0884EB0008, 3M Reference No. T - 3 9 4 3 _____________________________________________________ I m m u n o t o x i c i t y ____________________________________________________________________ Guinea Pig Maximization - Method, Summary, and Raw Data Appendix, ______ Hazleton Labs, Project No. 40703984, 3M Reference No. T-3609________ Page 19 000024 3M Fluorochemical EPA Submissions G e n o t o x i c i t y _____________________________________________________________________________ Chromosomal Aberration Study of Sample D-l in Cultured Mammalian Cells, Mitsubishi Chemical Safety Institute, Study No. 2L162, 3M Reference No. T-6322.3 (FX-13)__________________________________ Mutagenicity Testing of 2-[N-ethyl-N-perfluoroalkyl (C=l~8)sulfonylamino]ethyl acrylate in Bacterial Reverse Mutation ______ Assays, BML, Study No. 2862, 3M Reference No. T-6322.5 (FX-13) In Vitro Microbiological Mutagenicity Assays of 3M Company's ______ Compound T-3609, SRI Inti, Project No. LSC-3145 _________________ Repeated-Dose Toxicity________________________________________________________ Twenty-Eight-Day Repeated Dose Oral Toxicity Study of Sample D-l in Rats, Biomedical Research Labs, Study No. BMR143C, 3M Reference No. T-6322 (FX-13)__________ N-EtFOSEMA_______________________________________________________________________________________ Acute Toxicity_________________________________________________________ ______________________________ Acute Oral Toxicity Screen with T-3494 in Albino Rats, Safety Evaluation Lab, Riker Labs, Project No. 0884AR0013, 3M Reference No. T-3494 (L- 1048, FX-14)__________________________________________________ Primary Skin Irritation Test with T-3494 in Albino Rabbits, Safety Evaluation Lab, Riker Labs, Project No. 0884EB0012, 3M Reference No. T-3494 (L-1048, FX-14) ___________________________________ Acute Ocular Irritation Test with T-3494 in Albino Rabbits, Safety Evaluation Lab, Riker Labs, Project No. 0884EB0011, 3M Reference No. T-3494 (L-1048, FX-14) ___________________________________ Immunotoxicity__________________________________________________________________________ _ Guinea Pig Maximization, Hazleton Lab, Project No. 40703985, 3M Reference No. T-3610 (NB No, 63601-32, FX-14)____________________ Genotoxicity________________________________________________________________________________ In Vitro Microbiological Mutagenicity Assays of 3M Company's Compound T-3610, SRI Inti, Project No. LSC-3145, 3M Reference No. T- 3610 (NB No. 63601-32, FX-14)__________________________________ N-MeFOSEA_________________________________________________________________________________________ Genotoxicity__________________________________________________________________________________________ Evaluation of the Mutagenic Activity of T-5869 in the Ames Salmonella / Microsome Test (with independent repeat), NOTOX, Project No. 115965, ______ 3M Reference No. T-5869.1 (FMZ-3559, Lot 2408, wide-range)_________ Evaluation of the Ability of T-5869 to Induce Chromosome Aberrations in Cultured Human Lymphocytes (with independent repeat), NOTOX, Project No. 115943, 3M Reference No. T-5869.2 (FMZ-3559, Lot 2408, ______ wide-range)____________________________________________________ Evaluation of the Mutagenic Activity of T-5869 in an In Vitro Mammalian Cell Gene Mutation Test with L5178Y Mouse Lymphoma Cells (with independent repeat), NOTOX, Project No. 115954, 3M Reference No. T- 5869.3 (FMZ-3559, Lot 2408, wide-range)__________________________ Page 20 000025 3M Fluorochemical EPA Submissions p f o s ___________________________________________________________________________________________________ Acute Toxicity_____________ ________________________________ ___________________________________ ___ Acute Oral Toxicity Study of T-6684 in Rats (OECD Guidelines), Coming ______ Hazelton, Project No. CHW 61101149, 3M Reference No. T-6684_______ Primary Dermal Irritation / Corrosion Study of T-6684 in Rabbits (OECD Guidelines), Coming Hazleton, Project No. CHW 61101150, 3M ______ Reference No. T-6684_______________ ___________ _______________ Primary Eye Irritation / Corrosion Study of T-6684 in Rabbits (OECD Guidelines), Coming Hazelton, Project No. CHW 61101151, 3M ______ Reference No. T-6684_______________________________________ Final Report, Acute Oral Toxicity Study of T-5898 in Rats, Hazelton, Study No. 40200468_____________________________________________ Final Report, Primary Eye Irritation / Corrosion Study of T-5898 in ______ Rabbits, Hazelton, Study No. 40200469_____ _______________________ Final Report, Primary Dermal Irritation / Corrosion Study of T-5898 in ______ Rabbits, Hazelton, Study No. 40200470_________________________ _ Acute Oral Toxicity - Rats, Biosearch, 3M Reference No. T -1388 ______ (perfluorooctanesulfonic acid)________________________________ _ Qualitative Investigation of the In Vitro Metabolism of T-6292, T-6293, T-6294 and T-6295 by Rat and Human Hepatocytes Using Ion Spray LC/MS and LC/MS/MS, Advanced Bioanalytical Services [Preliminary] ______ Analytical Report, Report 96ADEM01.3M__________________________ Teratology_____________________________________________________________________________________________ Memo from E.G. Lamprecht re Fetal Rat Lens Artifact - Summary of ______ Developments_______________________________________________ Analytical_____________________________________________________________________________________________ Ion Spray LC/MC Determination of Perfluoro Analytical Standards Provided by 3M Medical Department, Advanced Bioanalytical Services, No. 9SMYHW01.3M____________________________________________ Studies in Progress_________________________________________________________________________________ Protocol, Feces Method Development Metabolism Study for Perfluorooctanesulfonate Derivatives, 3M Strategic Toxicology Lab, Study Nos. T6316.17; T-6295.21; T-7132.3; ST-41____________________ Protocol, Cell Proliferation Study with N-Ethyl Perfluorooctanesulfonamido Ethanol (N-EtFOSE; 3M T-6316.11), Perfluorooctane Sulfonic Acid Potassium Salt (PFOS; 3M T-6295.16), and N-Ethyl Perfluorooctanesulfonamide (PFOSA 3M T-7091.1) in Rats, ______ Pathology Associates Inti, Study No. 1132-100_______________________ Page 21 000026 CD No. 2 3M Fluorochemical EPA Submissions May 23 2000 ____________________________________________________ _ Cover Letter to EPA Net-FOSE______________________________________________________________________ _____ _____________ __ Summary Octanol / Water Partition Coefficient / Report - Determination o f the Partition Coefficient (N-Octanol / Water) of T-5874 by High ____________ Performance Liquid Chromatography (HPLC) ____________________ __________Perfluorodecansulfonate, Ammonium Salt (PFDS)______________________________________ __________Environmental Fate and Transport_______________________________________________ _ ____________ BOD/COD Testing Results FC-120______________________________ _ ____________ BOD/COD Testing Results FC-121-X_____________________________ __________Ecotoxicity Elements_____________________________________________________________________________ ____________ Acute Toxicity of E2566-1 to Daphnia magna (FC-121-X)_____________ Static Acute Toxicity o f FC-120 to the Fathead Minnow, Pimephales ____________ promelas_____________________________________________________ ____________ Static Acute Toxicity o f FC-120 to the Daphnid, Daphnia magna________ OECD Activated Sludge Respiration Inhibition Test #209 - Toxicity o f ____________ FC-120__________________________________________________ __ ____________ Microhms* Microtex Test - FC-120________________________ M ethyl FOSEA___________________________________________________________________________________ __________Physical / Chemical Properties______________________________________________________ _ Study o f Stability o f MeFOSEA in Aqueous Buffers Using Gas ____________ Chromatography with Atomic Emission Detection___________________ __________Environmental Fate and Transport__________________________________________________________ ____________ Determination o f the Partition Coefficient (N-Qctanol/Water) o f T-5869.4 __________Perfluorooctane sulfonylamido (ethyl) acetate = PFOSAA_____________________________ __________Environmental Fate and Transport__________________________________________________________ ____________ Ready biodegradability ofFC-109-X - BOD/COD___________________ _________ Ecotoxicity Elements______________________________________________________________________________ ____________ Acute Toxicity o f E2566-2 to Fathead Minnow (Pimephales promelas) ____________ Acute Toxicity o f E2566-2 to Daphnia magna___________ ____________ ____________ Activated Sludge Respiration Inhibition - FC-109-X__________________ ____________ Microbics Microtox Toxicity T est- FC-109-X______________________ ____________ Acute toxicity o f FC-127 to fathead minnow________________________ ____________ Toxicity o f FC-127 to activated sludge respiration____________________ _________ EnviranmentakFate and T ransport_________________________________________________________ B ^ r^ tB S gad ab ility o f FC-127 - BOD/COD Tfe^iiKqtOTt- Fate o f Fluorochemicals in the Environmental (sic) _______ (Wai&uirircspiromcter method for biodegradability o f FC-128)_________ __________ ^ ^ B iod egrad ab ility o f FC-128 - BOD/COD_____________________ EcotoxictyElements -- ____________ Acute toxicity o f FC-128 to fathead minnow________________________ ____________ Acute toxicity o f FC-128 to fathead minnow________________________ ____________ Acute toxicity o f FC-128 to fathead minnow________________________ ____________ R1904 BOD Report for 3M Company_____________________________ ____________ Biodegradation (methylene blue active substance)____________________ Page 22 000027 3M Fluorochemical EPA Submissions Ready Biodegradability: "Modified OECD Screening Test" for Fluorad FC-129_______________________ ________________________________ ______ Acute Toxicity o f R1904 to Fathead Minnow (Pimephales promelas) Growth Inhibition o f R1904 for Green Alga (Selenastrum capricomutum) Microbics Microtox Toxicity Test - FC-129________________________ Ethyl FOSEA____________________________________________________________________________ Physical / Chemical Properties______________________________________________________ __ ______ Determination o f Physico-chemical Properties of Sample D -l__________ Environmental Fate and Transport________________________________________________ Ready biodegradability o f FX-13 (BOD/COD)______________________ Ecotoxicity Elements________________________________________________________________________ __ ______ Acute toxicity o f FX-13 to fathead minnow_________________________ ______ Activate Sludge Respiration Inhibition Test_________________________ Environmental Fate and Transport_______________________________________________ ______ Bioaccumulation Study o f Sample D -l with Carp (Cyprinus carpio) May 25,2000______________________________________________________________ ______ Cover Letter to EFA_______________________________________________ __ ______ Study Summary for Work Performed During 1977-1979_____________________ __________ Toxicology Studies and Other Information on PFOA________________________________ PFOA_______________________________________________ ^ _________________________________________________ _________ Acute Toxicity____________________________________________________________________________________ Final Report, An Acute Inhalation Toxicity Study o f T-2305 CoC in the ____________ Rat, Bio/dynamics, Study No. 78-7184______________________________ Technical Report Summary, Analysis o f rat serum o f FC-143, reported in ____________ terms o f organic and inorganic fluorine, Central Research Labs__________ Final Report, Acute Oral Toxicity (LD50) Study in Rats, Inti R&D Corp, ____________ Study No. 137-091,3M Reference No. Fluorad FC-143, Lot 340________ Final Report, Acute Oral Toxicity Study o f T-6669 in Rats, Coming ____________ Hazelton, Study No. CHW 61001760,3M Ref. FC-143________________ Final Report, Primary Skin Irritation Study - Rabbits, Biosearch, 3M ____________ Reference No.T-1395, FC -143___________________________________ Final Report, Primary Eye Irritation Study - Rabbits (Washout ____________ Procedure), Biosearch, 3M Reference No. T-139S, FC-143_____________ FinalKfe(icg$ Primary Eye Irritation Study - Rabbits (Not Washed), ____________ B fe s c a a llik Reference No. T-1395, FC-143_______________________ . FinahRfeporLPrimary Skin Irritation Test with T-3371 in Albino Rabbits, ____________ RatsfeEahr, Study No. 0883EB0079,3M Reference FC-26, Lot 389 FinaiKeport, Acute Oral Toxicity - Rats, Biosearch, 3M Reference No. T____________ 15851FC-26 (perfluorooctanoic acid)_______________________________ Final Report, Primary Eye Irritation Study - Rabbits, Biosearch, 3M ____________ Reference No. T-1583, FC-26 (perfluorooctanoic acid)________________ Final Report, Acute Dermal Toxicity Study o f T-6342 in Rabbits, Coming Hazelton, Study No. Hwi50800374,3M Ref. No. FC-I090, L13364, Lot 1, ____________ F11426 sodium perfliiorooctanoate solution_________________________ Page 23 000028 3M Fluorochemical EPA Submissions Genotoxicy________________ ___________________________________________________________ An Assay o f Cell Transformation and Cytotoxicity in the C3H 10T l/i ClonalCell Line for the Test Chemical T-2942 CoC, Environmental Pathology Lab, Stone Research Lab, 3M Ref. No. FC-143 Lot 340, L138679______________________________________________________ Final Report, Mutagenic Evaluation o f T-20I5 C6C in the Ames Salmonella/Microsome Plate Test, Litton Bionetics, Study No. 20838, 3M Ref. No. FC-143_____________________________________________ __ Final Report, Mutagenicity Test on T-6564 in an In Vivo Mouse Micronucleus Assay, Coming Hazelton, Study No. 17750-0-455, 3M Ref. No. FC-1015, L-13167, straight-chain APFO________________________ Final Report, Mutagenicity Test on T-6564 Measuring Chromosomal Aberrations in Chinese Hamster Ovary (CHO) Cells: with a Confirmatory Assay with Multiple Harvests, Coming Hazelton, Study No. 17750-0______ 437CO, 3M Ref. No. FC-1015, L13167, straight-chain APFO___________ Final Report, Mutagenicity Test with T-6564 in the Salmonella Escherichia Coli / Mammalian - Microsome Reverse Mutation Assay with a Confirmatory Assay, Coming Hazelton, Study No. 17750-0-409R, 3M ______ Ref. No. FC-1015, L13167, straight-chain APFO_____________________ Final Report, Mutagenicity Test on T-6342 Measuring Chromosome Aberrations in Human Whole Blood Lymphocytes with a Confirmatory Assay with Multiple Harvests, Coming Hazelton, Study No. 17073-0449CO, 3M Ref. No. FC-1090, L13364, FI 1426, Lot 1, sodium ______ perfluorooctanoate______________________________________________ Final Report, Mutagenicity Test on T-6342 Measuring Chromosomal Aberrations in Chinese Hamster Ovary (CHO) Cells: with a Confirmatory Assay with Multiple Harvests, Coming Hazelton, Study No. 17073-0437CO, 3M Ref. No. FC-1090, LI3364, FI 1426, Lot 1, sodium ______ perfluorooctanoate_________________ .____________________________ Final Report, Mutagenicity Test on T-6342 in an In Vivo Mouse Micronucleus Assay, ComingHazelton, Study No. 17073-0-455,3M Ref. ______ No. FC-1090, L13364, F11426^ Lot 1, sodium perfluorooctanoate_______ Final Report, Mutagenicity Tbst with T-6342 in the Salmonella Escherichia Coli / Mammalian--Microsome Reverse Mutation Assay, Corningi&zelton, Study No. 1703073-0-409,3M Ref. No. FC-1090, E 1 3 iliB lH 4 2 6 , L oti, sodium Perfluorooctanoate____________________ * ' Voliimes 1-4, Two Year Oral (Diet) Toxicity / St udy o f Fluorochemical FC-143 in Rats, Riker Labs, '-SfedjSiw0281CR0012________________________________________ FinalReport, Ninety Day Subacute Rat Toxicity Study on Fluorad Fluorochemical FC-143, Inti R&D Corp, Study No. 137-089,3M Reference No.T-3141 _________________________ 3M Internal Correspondence re FC-143 analysis o f serum samples from rats o f 90-day subacute study, from Jon Belisle to JE Long, dated March 2 0 ,1 9 7 $ _______________________________________________ Page 24 000029 3M Fluorochemical EPA Submissions 3M Internal Correspondence re FC-143 analysis of liver samples from rats o f 90-day subacute study, from Jon Belisle to JE Long, dated March 31, 1978_________________________________________________________ Final Report, 28-Day Oral Toxicity Study with FC-143 in Albino Mice, Industrial Bio-Test Labs, Study No. 8532-10655, 3M Reference No. T-1742CoC, Lot 269_______________________ _____________________ Final Report, 28-Day Oral Toxicity Study with FC-143 in Albino Rats, Industrial Bio-Test Labs, Study No. 8532-10654, 3M Reference No. T-1742CoC, Lot 269____________________________________________ Final Report, Repeat Application 28 Day Percutaneous Absorption Study with T-2618CoC in Albino Rabbits, Safety Evaluation Lab, Riker Labs, Study No. 09790AB0485,3M Ref. No. FC-143______________________ Final Report, Ninety Day Subacute Rhesus Monkey Toxicity Study, Inti R&D Corp, Study No. 137-090 _________________________________ Analytical data, FC-143 Analysis o f IRDC 137-090 Samples [monkey ______ serum and liver samples], 3M Central Research Labs__________________ Final Report, Vol. I-U, 13-Week Dietary Toxicity Study with T-5180, Ammonium Perfluorooctanoate (CAS No. 3825-26-1) in Male Rats, ______ Hazelton, Study No. HWI6329-100________________________ _ Final Report, 5 Daily Dose Oral Toxicity Study with T-6669 (FC-143) in ______ Rats, Coming Hazelton, Study No. CHW 6329-197___________________ Ongoing____________________________________________ |___________________________________________ __ 26-Week Capsule Toxicity Study with Ammonium Perfluorooctanoate (APFO) in Cynomolgus Monkeys, Covance Labs, No. 6329-231, 3M Ref. ______ No. T-6889.3. In Progress. Protocol provided. Pharmacokinetic Studies_________________________________________________________________________ Final Report, Analytical Study, Single-Dose Intravenous Pharmacokinetic Study o f T-6067 in Rabbits, 3M Ref. No. LI3492, Study No. AMDT120694.1; report includes Final Report, Single-Dose Intravenous Pharmacokinetic Study o f T-6067 in Rabbits, Hazelton, Study No. HWI 6329-151______________________________________________________ Final Report, Analytical Study, Single-Dose Absorption/Toxicity Study o f T-6067, T-6068, and T-6065Hn Rabbits, 3M Environmental Technology & Services, Study No. AMDT-011095.1; report includes Final Report, SingkfrDbfg Absorption/Toxicity Study o f T-6067, T-6068, and T-6069 in R a b ^ M ^ elto n , Study No. HWI 6329-152________________________ FihakK^fi^ Absorption o f FC-143-*4C in Rats After and Single Oral ______ DoscgRfli;Labs_______________________________________________ FmalRlport, Synthesis and Characterization o f FC-143-14C, Commercial ______ Chemicals Division and Riker Labs________________________________ Final Report, Extent and Route o f Excretion and Tissue Distribution o f Total Carbon-14 in Male and Female Rats After a Single IV Dose o f FC143-*4C, Riker Labs____________________________________________ Page 25 000030 3M Fluorochemical EPA Submissions Final Report, Extent and Route o f Excretion of Total Carbon-14 in PregnantRats After a Single Oral Dose o f Ammonium 14C______ Perfluorooctanoate, Riker Labs________________________ _ _ _ _ _ _ _ _ Final Report, Enhanced Elimination o f FC-95-14C and FC-143-14C in ______ Rats with Cholestyramine Treatment, Riker Labs_____________________ Final Report, Single-Dose Intravenous Pharmacokinetic Study o f T-6564 ______ in Rabbits, Coming Hazelton, Study No. CHW 6329-186______________ Final Report, 5-Daily Dose Dermal Absorption/Toxicity Study o f T-6564 ______ in Rabbits, Coming Hazelton, Study No. CHW 6329-185______________ Teratology__________________________________________________________________________________ _ Oral Rangefinder Study o f T-2998CoC in Pregnant Rats, Riker Labs, Study No. 0680RR0018___________________________________ _ _ _ Final Report, Oral Teratology Study o f T-2998 CoC in Rats, Riker Labs, ______ Study No. 0681TR0110, with Amendment to Final Report and Protocol Oral Rangefinder Study o f T-3141 CoC in Pregnant Rabbits, Riker Labs, Study No. 0681RB0331__________________________________________ Final Report, Oral Teratology Study o f T-3141 CoC in Rabbits, Riker Labs, Study No. 0681TB0398_____________________________________ 3M Internal Correspondence re analytical analyses o f T-3141 CoC, from ______ L.D. Winter to D.R. Ricker____________________________________ _ Letter re further testing from E. Marshall Johnson to Dr. Franklin D. ______ Griffith______________________________________________ _ 3M Memo re FC-95 and 143 Teratology, from Marv Case to Geoijean ______ Adams, enclosing an analysis of the eye lens defect___________________ Human Sera/M edical Surveillance/Epidemiology______________________________________ F.A. Ubel, et al. Health Status o f Plant Workers Exposed to Fluorochemicals - A Preliminary Report, 41 Am. Ind. Hyg. Assoc. J. 584______ 589 (August 1980), with Summary.________________________________ Leonard M. Schuman & Jack S. Mandel, An Epidemiologic Mortality Study o f Employees at the Chemolite Plant (February 1980), with ______ Summaryo f study.______________________________________________ Jack S. Mandel & Leonard M. Schuman, "Mortality Study at the 3M ______ Chemolite Plant" (January 1989), with Summary o f study.______________ FrankELGilliland & Jack S. Mandel, Mortality Among Employees o f a * P eif^ ^ H M m ic Acid Production Plant, 35 JOM 950-954 (September iS W ^ ^ R u n m a r y o f study.____________________________________ . FranfcDayi&Gilliland, Fluorocarbons and Human Health: Studies in an Ofeg r t iB ttC d w tt (October 1992) (unpublished Ph.D. thesis, University ______ with Summary._________________________________________________ Geary-WtOBen, et al., An Epidemiological Investigation o f Reproductive Hormones in Men with Occupational Exposure to Perfluorooctanoic Acid, 40 JOEM614-619 (July 1, 1998), with Summary o f study._____________ Page 26 000031 3M Fluorochemical EPA Submissions Frank D. Gilliland & Jack S. Mandel, Serum Perfluorooctanoic Acid and Hepatic Enzymes, Lipoproteins, and Cholesterol: A Study o f Occupationally Exposed Men, 29 American Journal o f Industrial Medicine 560-568 (1996), with Summary o f study._________ Geary W. Olsen, et al., An Epidemiologic Investigation of Plasma Cholecystokinin and Hepatic Function in Perfluorooctanoic Acid Production Workers, 3M Final Report EPI-0003, (1997), with Summary of study, Protocol, and Manuscript accepted for publication in 2000, Drug & ______ Chemical Toxicology.____________________ _______________________ Physicians Plant Summary Reports Re Medical Surveillance Program at ______ Cottage Grove (1978-1991)___________________________________ __ _____________Blood Levels o f RF/F in Selected Employees__________________ 3M Internal Correspondence re 1981 Chemical Division Health _____________Evaluations from D.E. Roach to P.F. Riehle____________________ 3M Internal Correspondence re 1981 Chemolite Health _____________Evaluations, from D.E. Roach to P.F. Riehle___________________ 3M Internal Correspondence re Inorganic Fluoride Determinations, _____________from D.E. Roach to P.F. Riehle______________________________ 3M Internal Correspondence re Organic Fluorine Levels, from _____________D.E. Roach to P.F. Riehle______________________________ _ 3M Internal Correspondence re Medical Examinations, from L. ____________ Zobel to D.W. Dworak ________________________________ 3M Internal Correspondence re Chemolite Evaluations, Fall 1990, ____________ from Larry R, Zobel to Clair Murphy, et al.____________________ PFO S___________________________________________________________________________________________________ Letter from E. Marshall Johnson to William C. McCormick, HI, re ______ Hazelton teratology studies_______________________________________ Letter from E. Marshall Johnson to William C. McCormick, III re Riker ______ teratology studies on PFOS and ethyl FOSE _______________________ IV-EtFOSEalcohol_______________________________________________ Letter from E. Marshall Johnson to William C. McCormick, III re Riker ______ Teratology studies on PFOS and ethyl FOSE_________________________ FinalReport, Biological Phase, Metabolism o f N-Ethyl Perfluorooctane Sulfanomido Ethanol (N-EtFOSE; T-6316) In Cynomolgus Monkeys ^ ^ ^ ^ ^ dministratinn o f a Single Capsule Dose, Covance, Study No. P F o m m ^ ^ m n u tsa m : P K vsicaioe oe m icatProperties_______ Wjfeacetone and toluene solubility estimates - FC-143 EnvironmentalFate and Transport__________________________________ Ready biodegradation of FC-143 (BOD/COD/TOC)___________________ Technical Report Summary - Adsorption o f FC-95 and FC-143 on Soil, ______ anda critique from Dr. S. Boyd, Michigan State University_____________ Technical Report Summary --Biodegradation Studies o f Fluorocarbons ffl (FC-143)___________________________________________________ Page 27 000032 3M Fluorochemical EPA Submissions Technical Report Summary - FC-143 Photolysis Study Using Simulated ______ Sunlight__________________________________________ _ Technical Report Summary - Analysis for Fluorochemicals in Bluegill Fish, and a critique by J.W. Gillett o f Cornell University______________ ______ Ready biodegradation o f FC-143 (BOD/COD)______________________ ______ Calculated octanol water partition coefficient - FC-143_______________ ______ Ready biodegradation of FX-1001 (BOD/COD)_____________________ ______ Ready biodegradation o f FC-126 (BOD/COD)______________________ Technical Report Summary - Assessment o f the Bioaccumulative ______ Properties o f Ammonium Perfluorooctanate: Static (fish test)__________ Ecotoxicity Elements____________________________________________ ______ 96-hour acute toxicity to fathead minnow- FC-143___________________ ______ 96-hour acute static toxicity to fathead minnow - FC-26______________ ______ 96-hour acute static toxicity to bluegill sunfish - FC-143______________ ______ 96-hour acute static toxicity to bluegill sunfish - FC-143______________ Two Reports: "The Effects o f Continuous Aqueous Exposure to 78.03 on Hatchability o f Eggs and Growth and survival of Fry of Fathead Minnow (Pimephales promelas)" and "Summary o f Histopathological Examinations o f Fathead Minnow (Pimephales promelas) Exposed to 78.03 for 30 Days"_____________________________________________ Technical Report Summary - Aquatic Toxicity Testing: FC-143 ( Lot-37) L.R. 5625 S___________________ ______ Toxicity to activated sludge - FC-143______________________________ Technical Report Summary - Multi-Phase Exposure/Recovery Algal ______ Assay Test method (FC-143)_____________________________________ 48-hour acute static toxicity to Daphnia magna - FC-143 (3 studies on ______ diet)________________________ Chronic Toxicity o f Fluorochemicals to Daphnia magna (Water Flea) ______ FC-143, and statistical analysis o f data_____________________________ ______ 96-hour acute static toxicity to fathead minnow - FX-1001____________ ______ Activatedsludge respiration inhibition (OECD 209) - FC-126_________ ______ M ircotoxtest- FC -126_________________________________________ ______ 48-hour acute static toxicity to Daphnia magna - FC-126______________ ______ 96-hour acute static toxicity to fathead minnow - FC-126_____________ ______ Activatedsludge respiration inhibition (OECD 209) - FX-1003________ M ierotottcst-FX-lQ03 SftafaiAcartfcToxicity to die Fathead Minnow, Pimephales promelas ______ SlodfeAcuteToxicity o f FX-1003 to the Daphnid, Daphnia magna______ 1 G lbu^d^R espiration Toxicity Test with N2803-3 and the Freshwater a^aj^tefettum capricomutum__________________________________ ______ Acute Toxicity o f N2803-3 to thc Fathead Minnow, Pimephales promelas ______ Acute Toxicity o f N2803-3 to the Daphnid, Daphnia magna____________ ______ M ircotoxtest--FC -143_________________________________________ ______ Microtox te s t- FC-11 8 _________________________________________ Microtox te s t- F C - 1 0 1 5 - X __________________________ Page 28 000033 3M Fluorochemical EPA Submissions ______ Activated Sludge Respiration Inhibition Test -FC -1015-X________ _ ______ Acute Toxicity o f FC-1015 to the Fathead Minnow, Pimephaies promelas Growth and Reproduction Toxicity Test with FC-1015 and the Freshwater alga, Selenastrum capricomutum________________________________ ______ Acute Toxicity of FC-1015 to fee Daphnid, Daphnia Magna __________ PFOS didecyldimethylammonium s a lt____________________________________________ Environmental Fate and Transport_____________________________________________ Determination o f the 28-day Carbonaceous Biochemical Oxygen Demand (CBOD) for P3025_____________________________________________ Ecotoxicity Elem ents________________________________________________________________ Acute Toxicity o f P3025 Developmental Material to Fathead Minnow ______ (Pimephaies promelas)__________________________________________ ______ Acute Toxicity o f P3025 Developmental Material to Daphnia magna ______ Growth Inhibition o f P302S Green Alga (Selenastrum capricomutum) ______ Growth Inhibition o f P302S to Green Alga (Selenastrum capricomutum) ______ Inhibitory Effects o f P3025 on Microtox____________________________ ______ Inhibitory Effects o f P3Q25 on Activated Sludge Respiration___________ May 30,2000_____________________________________________ ______ Cover Letter to EPA__________________________________ __________ Voluntary Use and Exposure Information.Profile______________ ____________ Perfluorooctanesulfonyl fluoride__________________ ____________ Perfluorooctanesulfonamide_____________________ ____________ Perfluorooctane sulfonylamido (ethyl) acetate_______ ____________ Perfluorodecanesulfonate_______________________ ____________ N-ethyl perfluorooctanesulfonamide_______________ ____________ N-methyl perfluorooctanesulfonamide_____________ ____________ N-ethylperfluorooctane sulfonamidoethanol________ N-methylperfluorooctane sulfonamidoeth'anol ____________ N-ethylperfluorooctanesulfonamidoethyl acrylate ____________ N-ethylperfluorooctanesulfonamidoethyl methacrylate ____________ N-methyl perfluorooctanesulfonaxnidoethyl acrylate June 8,2000 _____________________________________________ ______CoveeDadetlEriPBfc-________ ______ IB e atdIBrptUCTMIfonnation Profile forperfluorooctanoic acid and salts J u n e 2 8 |2 fl0 flfe ______ CoverfcctterhyEPA_________________________________________________ ToxicoISgtcalStudies and OtherRelevantInformation on PFOSMixtures ~ Genotoxicity__________________ ____________________________________________________________________________________________________________________________________________ Final Report, In Vitro Microbiological Mutagenicity Assays o f Three 3M Company Compounds, 3M Reference Nos. T-2136 [FC-206], T-2138, T- ____________ 2140, SRI Inti, Project No. LSC 4442-16__________________________ Page 29 000034 3M Fluorochemical EPA Submissions Final Report, In Vitro Microbiological Mutagenicity Assays o f 3M ______ Company's Compound T-3651, SRI Inti, Project No. LSC-3145 PFOS M ixtures ______________________________________________________________________________ F C -18S F ____________________________________________________________________________________ Environm ental F ate and Transport________________________________________________________ Ready biodegradability o f FC-185F (BOD/COD)____________________ Ecotoxicity Elem ents_________________________________________________________________ Aquatic Toxicity Testing: FC-185F (cc817-19) L 1-2303 LR 7544 ______ (Fathead Minnow)_____________________________________________ ______ Acute toxicity o f FC-18SF to activated sludge mixed liquor____________ F C -2 0 0 _______________________________________________________________________________________________ Summary Reports___________________ Summary fos work performed for the German Government Agency for Military Technology and Procurement on fire extinguishing foam compounds from multiple companies FC-201________________________________________________________________________________________________ Environm ental Fate and Transport__________________________________________________________ ______ Ready biodegradability o f FC-201 (BOD/COD)_____________________ E cotoxicity Elem ents_____________________________________________________________________________ ______ Acute static toxicity o f FC-201 to fathead minnow___________________ FC-201AF_________________________________________ ..________________________________________________ Environm ental Fate and Transport__________________________________________________________ ______ Ready biodegradability o f FC-201AF (BOD/COD)___________________ ______ Ready biodegradability o f FC-201AF (BOD/COD)___________________ E cotoxicity Elem ents_____________________________________________________________________________ ______ Acute static renewal o f FC-201AF to fathead minnow________________ ______ Acute toxicity o f FC-201AF to green algae, Selenastrum capricomutum Growth Inhibition Test with the Marine Alga Skeletonema coastatum - ______ FC-201 AF Light Water Brand AFFF______________________________ AcuteToxicity Test with the Marine Copepod Acartia tonsa - FC-201 AF ______ Light WaterBrand AFFF________________________________________ AcuteToxicity Sediment Test with the Marine Amphipod Corophium ______ volutator- FC-201 AF LightWater Brand AFFF____________________ ______ Acute static toxicity o f FC-201AF to Daphnia magna_________________ Aeuha i ai i f e o f FC-201AF to Mircotox___________________________ Acu t e t b f e y o f FC-201AF to activated sludge mixed liquor -- Wp -- mi-- i n-- " 1 ... ............ ---- * ....-- - -- -- F C-20IF '$& Environm entaFFatcand Transport_________________________________________________________ Read^m&gradability o f FC-201F (BOD/COD)____________________ EcoUadcityfEtemente______________________ ______ Acute Toxicity o f FC-201F to Fathead Minnow (Pimephales promelas) Further Investigatiott on the Acute Toxicity o f FC-201F to Fathead ______ Minnow (Pimephales prom elas)_________________________________ ______ Acute Toxicity o f FC-201F to Daphnia magna______________________ ______ Microbial inhibition (dehydrogenase activity) o f FC-201F_____________ Page 30 000035 3M Fluorochemical EPA Submissions Acute toxicity to fish (leuciscus idus) FC-201F-X EnvironmentalFate and Transport Ready biodegradability o f FC-20IF-X (BOD/COD) Ecotoxicity Elements Acute toxicity o f FC-201F-X to Mircotox ______ Acute toxicity o f FC-201F-X to activated sludge mixed liquor FC-203 EnvironmentalF ate and Transport Summary o f biodegradability tests o f FC-203 (BOD/COD) OECD Biodegradation Testing o f FC-600 and FC-203 Repeat OECD Biodegradation Test on FC-600 and FC-203 Modified OECD Screening Test on FC-203, FC-203A, and FC-600 Ecotoxicity Elements Acute toxicity o f FC-203 to rainbow trout Acute toxicity o f Light Water to bluegill (Lepomis macrochirus)_________ Summary o f acute static toxicity tests o f FC-203 to bluegill_____________ Acute Toxicity o f 3M Samples (A and B) to the Water Flea (Daphnia magna) and Scud (Gammarus fasciatus) - robust summary o f Gammarus fasciatus_____________________________________________ _ Acute Toxicity o f 3M Samples (A and B) to the Water Flea (Daphnia magna) and Scud (Gammarus fasciatus) - robust summary for Gammarus fasciatus_______________________________________________________ Summary o f microbial inhibition (dehydrogenase activity) teste on FC-203 Algicidal Activity o f Two Samples Labeled "A" and "B" Acute static toxicity o f FC-203 to rainbow trout Acute toxicity o f 3M Company's sample 7902 to grass shrimp (Falaemonetes pugio)___________________________________________ Acute Toxicity o f 7902 to the Common Mummichog (Fundulus heteroclitus)__________________________________________________ Acute Toxicity o f 3M Company's Sample 7902 to embryos - larvae of eastern oysters (Crassostrea virginica)_____________________________ Acute toxicityofSam ple #5729 to embryo - larvae o f eastern oysters (Crassostrenvirginica)_________________________________________ bxicity o f FC-203 to the Alga, Selenastrum capricomutum o f FC-203 without Tofytirazole (VW160390) to Microtox `oxicity o f FC-203 to the Daphnid, Daphnia magna_______ Sediment Test with the Marine Amphipod Corophium ~Acia&^Tdiicity Test with the Marine Copepod Acartia tonsa ~ _______Growth Inhibition Test with the Marine Alga Skeletonema costatum Summary Reports _________________________________________________________________________ Investigations o f toxic effects and biological degradability o f foam _______extinguishing agents in the waste water____________________________ Page 31 000036 3M Fluorochemical EPA Submissions Environmental Effects o f LIGHT WATER Brand AFFF and Components ______ - Robust Summary for FC-203________________________________ Environmental Effects o f LIGHT WATER Brand AFFF and Components - Robust Summary for VW160390 (FC-203 without tolyltriazole)______ F C - 2 0 3 A _____________________________________________________________________________________ ___ Environmental F ate and Transport_______________________________________________ ______ Ready biodegradability o f FC-203A (BOD/COD)____________________ ______ Biodegradation o f `Tight Water" Products in OECD Test - 6/80________ ______ Effect o f Biodegradation o f Foaming o f "Light Water" Products________ ______ Modified OECD Screening Test o f FC-203, FC-203A, and FC-600_____ Ecotoxicity Elem ents_________________________________________________________________________ ___ ______ Acute toxicity ofFC-203A to fathead minnow______________________ F C -203C _____________________________________________________________________________________________ Environmental Fate and Transport________________________________________ ______ Ready biodegradability (BOD/COD) o f FC-203C____________________ E cotoxicity Elem ents_____________________________________________________________________________ ______ Acute Toxicity o f 81-1/8 to Fathead Minnow (Pimephales Promelas) Acute Toxicity o f a Surfactant Solution (81-1/8) to the Mummichog ______ Fundulus heteroclitus in a Flow-through System.____________________ ______ Algal Assay, Bottle Test (AA:BT), Toxicity Testing FC-203C_________ ______ Acute toxicity o f FC-203C to Daphnia magna_______________________ ______ Acute toxicity o f FC-203C to activated sludge mixed liquor___________ F C -203C E __________________________________________________________________________________________ Environm ental F ate and T ran sport_________________________________________________________ Ready biodegradability (BOD/COD) & TOC of FC-203CE____________ Ready biodegradability (OECP 3Q1B) o f FC-203CE_________________ E cotoxicity E lem en ts____________________________________________________________________________ ______ Acute static toxicity o f FC-203CE to Daphnia magna_________________ ______ Acute static-renewal toxicity o f FC-203CE to fathead minnow_________ ______ Acute toxicity o f FC-203CE to green algae, Selenastrum capricomutum Toxicity o f FC-203CE to Microtox Toxicity o f FC-203CE to activated sludge mixed liquor ToxicityoF-203CB + antifoamer to activated sludge mixed liquor Oxicity ofFC-203CB to the Daphnid, Daphnia magna xkntyfFC-203CE to the Alga, Selenastrum Robustsumroary o f FC-203CE FC-203CF EnvironmentalF ate and Transport E co to x icity E lem en ts Page 32 000037 3M Fluorochemical EPA Submissions Static Acute Toxicity o f FC-203CF to the Fathead Minnow, Pimephales Static Acute Toxicity o f FC-203CF to the Daphnid, Daphnia magna Acute toxicity o f FC-203CF to Microtox Static Acute Toxicity o f FC-203CF to the Alga, Selenastrum _______capricomutum____________________________ ____________________ _______Acute toxicity o f FC-203CF-X to activated sludge mixed liquor________ Acute toxicity o f FC-203CF-X to nitrifying bacteria (ISO/DIS Method 9509)_____________________________________________________ __ _______Acute toxicity o f FC-203CF-X to nitrifying bacteria (Nitroseed Method) _______Acute Toxicity o f FC-203CF to Fathead Minnow (Pimephales promelas) F C -293F C _________________________________________________________________________________________ Environmental F ate and Transport __________________________________________ _ ______ R1335 BOD Report for 3M Company_____________________________ E cotoxicity E lem ents_________________________________________________________________________ ___ ______ Toxicity o f FC-203FC to Mircotox_______________________________ ______ Toxicity o f FC-2Q3FC to activated sludge mixed liquor_______________ Acute Toxicity o f R1335 to Daphnia magna _______Growth Inhibition o f RI335 for Green Alga (Selenastrum capricomutum) _______Acute static toxicity o f FC-203FC to fathead minnow__________________ _______Acute Toxicity o f R1335 to FatheacLMinnow (Pimephales promelas) FC-203S_______________________________________________________ E cotoxicity Elem ents_______________________________________________________________________________ _______Acute toxicity o f FC-203S to Rainbow Trout_________________________ FC-206________________________________________________________ Environm ental F ate and T ransport___________________________________________________________ _______Ready biodegradability o f FC-206 (BOD/COD)_______________________ Ready biodegradability o f FC-206 (BOD/COD)_______________________ _______Biodegradation o f "LIGHT WATER" Products in OECD Test - 6/80 _______Effecto f degradation on foaming o f FC-206______ ___________________ E cotoxicity Elem ents _____________________________________________________________________________ _______Summary o f microbial inhibition (dehydrogenase activity) tests on FC-206 _______Acute8tatte^^ttHricityo f FC206 to fathead minnow____________________ toxicitydlEO -206to rainbow trout ofSampl&C to Munmrichog (Fundulus heteroclitus), aemonetesvulgaris) and Fiddler Crab (Uca pugilator) ofSample C to Mummichog (Fundulus heteroclitus), AcuteToxicity o f Sample C to Mununichog (Fundulus heteroclitus), Grass Shrimp (Palaemonetes vulgaris) and Fiddler Crab (Uca pugilator) Robust summary forfiddfercrab__________________________________ Acute Toxicity o f Sample C to Atlantic Oyster (Crassostrea virginica) Page 33 000038 3M Fluorochemical EPA Submissions Acute Toxicity o f Sample C to Water Flea (Daphnia magna) and Scud (Gammarus fasciatus) - Robust summary for Daphnia magna________ Acute Toxicity o f Sample C to Water Flea (Daphnia magna) and Scud (Gammarus fasciatus)--Robust summary for Scud_________________ Acute static toxicity ofFC -206 to fathead minnow Acute static toxicity o f FC-206 amended to soil to fathead minnow Acute flow-through toxicity ofFC-206 to bluegill sunfish________ Summary Reports Investigations o f toxic effects and biological degradability o f foam extinguishing agents in the waste water Summary for work performed for toe German Government Agency for Military Technology and Procurement on fire extinguishing foam _______compounds from multiple companies __________________________ FC-206A Environmental Fate and Transitori Ready biodegradability (BOD/COD) o f FC-206A Ecotoxicity Elements FC-206A cc 785-22: Results o f Aquatic 96-hr. LC50 Tests (bluegill sunfish) Acute static toxicity o f FC-206A to fathead minnow Acute flow-through toxicity o f FC-206A to fathead minnow Acute toxicity o f FC-206A to Daphnia magna Microbial inhibition (dehydrogenase activity) o f FC-206A Acute toxicity o f FC-206A to activated sludge mixed liquor FC-206C Environmental Fate and Trattsport Ready biodegradability (BOD/COD) o f FC-206C Ecotoxicity Elements Acute toxicity o f FC-206G to activated sludge mixed liquor _______Acute Toxicity o f 81-2/8 to Fathead Minnow (Pimephales promelas) Acute Toxicity o f a Surfactant Solution to the Mummichog (Fundulus _______heteroclitus) in a Flow-through System, (FC-206C)_______________ _______AlgalAssay: Bottle Test (AA:BT), Toxicity Testing FC-206C______ FC-206CE bility (BOD/COD) o fFC-206CE ility (OECD 3Q1E) o f FC-206CE Aeh^ sh ife toxicity o f FC-206CE to Daphnia magna________________ Aeuteitgtfc toxicity o f FG-206GE to Daphnia magna Acute static-renewal toxicity oFC-206CE to fathead minnow________ Acute toxicity o f FC-206CE to green algae, Selenastrum capricomutum Acute toxicity o f FC-206CE to M icrotox_________________________ Acute toxicity o f FC-206CE to activated sludge mixed liquor_________ Acute toxicity o f FC-206CE to activated sludge mixed liquor_________ Page 34 000009 3M Fluorochemical EPA Submissions ______ Acute toxicity o f FC-206GE + antifoamer to activated sludge mixed liquor ______ Static Acute Toxicity o f FC-206CE to the Daphnid, Daphnia magna_____ Static Acute Toxicity o f FC-206CE to the Alga, Selenastrum capricomutum__________________________________________________ Sum m ary R eports____________________________________________________________________________ ______ Environmental Effects o f LIGHT WATER Brand AFFF and components ______ - Robust summary for FC-206CB_______________________________ F C -206C F __________________________________________________________________________________ Environmental Fate and Transport ____________________________________________ __ ______ Ready biodegradability o f FC-206CF (BOD/COD)____________________ E cotoxicity Elem ents____________________________________________________________________________ Static Acute Toxicity o f FC-206CF to the Fathead Minnow, Pimephales ______ promelas______________________________________________________ ______ Static Acute Toxicity o f FC-206CF to the Daphnid, Daphnia magna______ ______ Acute toxicity o f FC-2Q6CF to Microtox____________________________ Static Acute Toxicity o f FC-206CF to the Alga, Selenastrum ______ capricomutum____________________________________________ _ ______ Acute toxicity o f FC-206CF-X to activated sludge mixed liquor_________ F C -600_________________________________________________________________________________________________ Environmental F ate and Transport _________________________________________________ ___ Summary o f ready biodegradability studies on FC-600, Lab Worksheets, ______ Tech Report Summaries__________________________________________ E cotoxicity Elem ents______________________________________________________________________________ ______ Acute static toxicity o f FC-600 to fathead minnow____________________ ______ Acute static toxicity o f FC-600 to bluegill sunfish_____________________ Acute static toxicity o f FC-600 to Daphnia magna - first o f 3 studies of ______ effluent from an FC -600- exposed SCAS reactor_____________________ Acute static toxicity o f FC-600 to Daphnia magna - second of 3 studies of effluent from an FC-600- exposed SCAS reactor (treated reactor after 4- ______ hours o f treatment)______________________________________________ Acute static toxicity o f FC-600 to Daphnia magna - third o f 3 studies o f effluent from an FC-60&- exposed SCAS reactor (blank control reactor ______ after 4-hours o f treatment)________________________________________ Acute,toxicity o f FC-600 to activated sludge mixed liquor______________ Anitcnftrraitv o f FC-600 (from Antwerp, Belgium) to activated sludge Ran|aiSwPg study o f acute toxicity o f FC-600 (US) to fathead minnow study o f acute toxicity o f FC-600 (Belgium) to fathead .............. ... -- ... ............ .... ................... R injlid^diag study o f acute toxicity o f FC-600 (US) to fathead minnow Range-finding study o f acute toxicity o f FC-600 (Belgium) to fathead minnow________ Dynamic Acute Toxicity o f3M-1 to Fathead Minnows (Pimephales promelas)_______________________ Aquatic Toxicity Testing FC-600 (Belgium-AFFF) LR 9595_________ Page 35 000040 3M Fluorochemical EPA Submissions ____________________________________________________________________________________________________________________________________________________ ____________ Ecotoxicity Elem ents____________________________________________________________________ ___ ______ Acute static toxicity ofFX-601 to fathead minnow________________ Acute toxicity o f FX-601 to activated sludge mixed liquor F C -6 0 2 ____________________________________________________________________________________ Environm ental F ate and Transport__________________________ ________________________ Ready biodegradability (BOD/COD) o f FC-602_____________________ E cotoxicity Elements_______________________________________________________________ ______ Toxicity o f FC-602 to Microtox_________________________________ ______ Acute static toxicity o f FC-602-X to Daphnia magna_________________ ______ Acute static toxicity o f FC-602-Xto fathead minnow________________ ______ Acute Toxicity Test with the Marine Copepod Acartia tonsa (FC-602) Growth Inhibition Test with the Marine Alga Skeletonema costatum FC-602)_____________________________________________________ Acute Toxicity Sediment Test with the Marine Amphipod Corophium ______ volutator (FC-602)_____________________________________ F C -603E F ____________________________________________________________________________________ Environmental F ate and Transport____________________________________________ _ ______ Ready biodegradability (BOD/COD) o f FC-603EF__________________ Ecotoxicity E lem ents__________________ ______________________________________________________ ___ ______ Acute toxicity of FC-603EF to the Qolden Orfe (Leuciscus idus)_______ ______ Acute toxicity o f FC-603EF to the alga, Scenedesmus subspicatus______ ______ Acute toxicity o f FC-603EF to Daphnia magna_____________________ ______ Bacterial dehydrogenase activity o f FC-603EF by the TTC method_____ ______ Toxicity o f FC-603EF to activated sludge__________________________ ______ Toxicity o f FC-603EF to Microtox_______________________________ ______ Toxicity o f FC-603EF to activated sludge mixed liquor_______________ Acute Toxicity of FC-603EF, Light Water TM ATC 3% AR-AFFF to ______ Daphnia magna_______________________________________________ Growth Inhibition o f FC-603EF, Light Water TM ATC 3%, AR-AFFF to ______ Great Alga (Selenastrum capricomutum)__________________________ Acute Toxidty o f FC-603EF, LightWater TM ATC 3% AR-AFFF to ______ Fathead Minnow (Pimephalca promelas)___________________________ GrowthlhhibitionTest with fee Marine Alga Skeletonema costatum - ^ A ea^ ^ ^ SfeX cstw ifethe Marine Copepod Acartia tonsa - FC-603EF A c |lii|^ E ^ ' sediment Testwith fee Marine Amphipod Corophium y O m tm ^ m -6 0 3 E __________________________________________________________________ . :___________________________________________________ Environmental-Fate and Transport .______________________________________________________ ______ Readybiodegradability (BOD/COD) o f FC-603F____________________ E cotoxicity E lem en t?___________________________________________________________________________ ______ Acute static toxicity o f FC-6Q3F to fathead minnow_________________ ______ Acute static toxicity o f FC-603P to Selenastrum capricomutum________ ______ Acute static toxicity o f FC-603F to Daphnia magna___________________ Page 36 000041 3M Fluorochemical EPA Submissions Toxicity o f FC-603F to M i c r o t o x ____________________ _ ______ Acute toxicity o f FC-603F to activated sludge mixed liquor_______ F C -7 8 0 ____________________________________________________________________ _______________ Environm ental Fate and Transport_____________________________________________ ______ Ready biodegradability (BOD/COD) o f FC-780______________________ Ecotoxicity Elem ents______________________________________________________________ Acute toxicity to Mummichog (Fundulus heteroclitus) F C -780B _____________________________________________________________________________________________ Environm ental F ate and Transport___________________________________________________________ ______ Ready biodegradabiiity o f FC-780B (BOD/COD)_____________________ ______ Ready biodegradabiiity o f FC-780B (AFFF C) (OECD Closed Bottle Test) Ecotoxicity Elem ents______________________________________________________________________________ 780B (lot 788-20): Results o f Aquatic 96 Hr. LC50 Tests Acute Toxicity o f 7901 to the Common Mummichog (Fundulus heteroclitus)___________________________________________________ Dynamic 96-Hour Acute Toxicity o f 3M Sample 1-3 to Fathead Minnows ______ (Pimephales promelas)_______ ______________________________ F C -783________________________________________________________________________________________________ Environm ental F ate and Transport_____________________________________________________ _ ______ Ready biodegradabiiity (BOD/COD) o f FC-783______________________ E cotoxicity Elem ents___________________________ ^_________________________________________________ Acute Toxicity o f 3M Sample No. 6 to Bluegill Sunfish (Lepomis ______ macrochirus)___________________________________________________ ______ Acute toxicity o f FC-783 to activated sludge mixed liquor______________ F C -787________________________________________________________________________________________________ Environm ental F ate and Transports___________________________________________________________ Ready biodegradabiiity o f FC-787 (BOD/COD)______________________ E cotoxicity Elem ents______________________________________________________________________________ ______ Acute toxicity o f FC-787 to Microtox_______ ^______________________ ______ Static Acute Toxicity offFC-787 to the Daphnid, Daphnia magna________ Flow ThroughAcute Toxicity o f FC-787 to the Fathead Minnow, ______ Pimephales promelas __________________________________________ F C -3003______________________________________________________________________________________________ E nviron m enta& B atm an dT ransport___________________________________________________________ ^ Company.. ~ - : to Microtox ________________________________ ______ TbfcfliitytafFC-3003 to activated sludge mixed liquor_________________ A j iiw il c h y o f R1331 toDaphnia magna GrowthThhibition o f R1331 for Greenalga (Selenastmm capricomutum)~ Acute static toxicity o f FC-3003 to fathead minnow___________________ ______ Acute Toxicity o f R1331 to Fathead Minnow (Pimephales promelas) F C - 3 0 1 7 _________________________________________ Environmental Fate and Transport _____________________________________________ ______ Biodegradation o f "Light Water" Products in OECD Test - 6/80________ Page 37 000042 3M Fluorochemical EPA Submissions N3688 BOD Report for 3M Company Ecotoxicity Elements Acutetoxicity ofFC-3017 to Microtox Acute static ienewai toxicity o f FC-3017 to fathead minnow Acute Toxicity o f FC-3017 to Daphnia magna Acute toxicity o f FC-3017 to Selenastrum capricomutum Summary Reports Summary for work performed for the German Government Agency for Military Technology and Procurement on fire extinguishing foam compounds from multiple companies____________________________ FC-303I Ecotoxicity Elements Acute static toxicity o f FC-3031 to fathead minnow FC-304I Environmental Fate and Transport Ready biodegradability (BOD/COD) o f FC-3041 Investigation for the Eco-toxicological Evaluation o f the Fluoro-surfactant Base FC-3041. Anaerobic Degradation. Part f _______________________ Investigation for the Eco-toxicologicai Evaluation o f the Fluoro-surfactant Base FC-3041. Anaerobic Degradation. Part g_______________________ Ecotoxicity Elements Acute static toxicity o f FC-3041 to fathead minnow Acute static toxicity o f FC-3041 to Daphnia magna Acute toxicity o f FC-3041 to activated sludge mixed liquor FC-3052 Ecotoxicity Elements Acute Toxicity o f FC-3052 to Rainbow Trout (Oncorhynchus mykiss) FC-3072 Ecotoxicity Elements AcuteToxicity of FC-3072 to Fathead Minnow (Pimephales Promelas) Acutestatic toxicity ofFC-3072 to Daphnia magna Acutntoxieity o f FC-3072 to Microtox f FC-3072 to activated shige mixed liquor (BOD/COD) o f FX-3103 o f FX-3103 to fatheadminnow toxicity o f FX-3103 to Selenastrum capricomutum Acute stade toxicity o f FX-3103 to Daphnia magna Toxicity o f FX-3103 to Microtox Acutettoxicity o f FX-3103 to activated sludge mixed liquor Page 38 00004a 3M Fluorochemical EPA Submissions Environm entalFate and Transport Ready biodegradability (BOD/COD) o f FX-3104 E ca to x icity E lem en ts A cutcstatic toxicity o f FX-3104 to fathead minnow Acute static toxicity ofFX-3104 to Seleuastrum capricomutum Acute static toxicity ofFX-3104 to Daphnia magna Toxicity ofFX-3104 to Microtox F x jm EnvironmentalFate and Transport Ready biodegradabiHty (BOD/COD) o f FX-3106 Ecotoxicity Elements Acutc static renewal toxicity o f FX-3106 to fathead minnow Toxicity o f FX-3106 to Microtox L-4647 EnvironmentalFate and Transport Ready biodegradability (BOD/COD) o f L-4647 Ecotoxicity Elements Acute static toxicity o f L-4647 to fathead minnow M iscellaneous Studies Summary for foaming studies done on various AFFF products Page 39 000044