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M&S.6 _ 066S PFOA Acute Toxicity - / 000814 gg) HAZLETON l a b o r a t o r e s a v e r c a ,SC 3301 KINSM AN BLVD., P.O. BO X 7S45 MADISON. Wl 53707 U SA FINAL REPORT Karen Armstrong 3M Toxicology Services Building 220-2E-02 St. Paul, MN 55144-1000 Sample: T-4416 HLA Number: 91201739 Enclosed: Acute Oral Toxicity Study in Rats (OECD Guidelines) o Key Personnel o Methodology o Summary of Results o Individual Pathology Comments o References o Pathology Report o Raw Data/Appendix Study Director Acute Toxicology PHONE (608) 241-4471 FACSIMILE (608) 241-7227 TELEX TLX 703956 HAZRAL MDS UD Date HAZLETON LABORATOR6 S AMEF1CA. SC. 3301 KIN SM AN BLVD., P.O. BO X 7545 MADISON. W l 53707 U SA HLA Number: 91201739 Sample: T-4416 Acute Toxicology Steven M. Glaza Study Director Section Supervisor Steven R. Sorenson Study Coordinator Patricia Padgham Group Leader Sharen L. Howery Report Coordinator Qua!itv Assurance Deborah L. Keller Manager Page 2 KEY PERSONNEL Laboratory Animal Veterinarian Cindy J. Cary, DVM Dipl ornate, ACLAM Anatomical Patholoav Thomas E. Palmer, PhD Anatomical Pathologist Dennis Hoffman Group Leader Necropsy Anne Mosher Group Leader Pathology Data PHONE (608) 241-4471 FACSIMILE (608) 241-7227 TELEX TLX 703956 HAZRAL MDS UD i oom njm ia Laboratory Sarveaa Company 000516 HAZLETON l a b o r a t o r c s a v e fic a ,rsic 3301 KIN SM AN BLVD., P.O. BO X 7S4S MADISON, W l S3707 U SA HLA Number: 91201739 Sample: T-4416 Page 3 METHODOLOGY Objective: To assess the acute oral toxicity produced when a test material is administered by oral gavage to rats.1 Test Material : T-4416 Physical Description: Purity and Stability: Storage and Retention: Safety Precautions: Clear colorless liquid Sponsor assumes responsibility for purity and stability determinations (including under test conditions). The test material was stored at room temperature. Any unused material will be returned to the Sponsor according to HLA Standard Operating Procedure. Normal handling procedures were used according to HLA Standard Operating Procedure. Test Animal: Adult albino rats, Crl:CDBR, were procured, separated by sex, maintained in group cages in temperature- and humidity-controlled quarters, provided continuous access to Rodent Chow 5001, Purina Mills, Inc., and water, and held for an acclimation period of at least 7 days. Animal husbandry and housing at HLA comply with standards outlined in the "Guide for the Care and Use of Laboratory Animals".2 If variations from the prescribed environmental conditions existed, they were documented and considered to have no effect on the study outcome. No contaminants were expected to have been present in the feed or water which would have interfered with or affected the results of the study. Acclimated animals were chosen at random for the study. Five male and five female rats, weighing from 204 to 299 g, were used for each dose level. The study consisted of three dose levels (0.2, 1.0 and 5.0 g/kg of body weight). Test animals were housed by sex in groups of five and identified by animal number and corresponding ear tag. Food and water were available ad libitum throughout the study, except for an overnight period just before test material administration when food, but not water, was withheld. Reason for Species Selection: Historically, rats have been used as a representative of a rodent species and are preferred by various regulatory agencies. Preparation and Administration of Test Material: The test material was administered as received. An individual dose of the undiluted material was calculated for each animal based upon its fasted body weight and administered by gavage. The dose volume of the test material varied for each dose level based upon the bulk density of the material of 1.28 g/mL. PHONE (606) 241-4471 FACSIMILE (608) 241-7227 TELEX TLX 703956 HA2RAL MDS UD O CW NW O Laboratory Sarvtcaa Company 000S 17  HAZLETON la b o r a t o r e s a m e b c a ,im c. 3301 KINSM AN BLVD., P.O. BO X 7545 MADISON. W l 53707 U SA HLA Number: 91201739 Sample: T-4416 Page 4 Reason for Route of Administration: Historically, the oral route has been the route of choice for administering a known amount of test material. Observations: The animals were observed for clinical signs and mortality at 1, 2.5 and 4 hours after test material administration. The animals were observed daily thereafter for 14 days for clinical signs and twice daily for mortality. Animals were weighed just before test material administration. Surviving animals were weighed again at Day 7 and at termination of the experimental phase or at death (when survival exceeded 1 day). Pathology: All animals whether dying on test or euthanatized at termination of the experimental phase were subjected to a gross necropsy examination and all abnormalities were recorded. After necropsy, the animals were discarded and no tissues were saved. Statistical Methods: The LDcg value for males, females and the combined sexes was calculated based upon tne observed mortality using a computer program utilizing a modified Behrens-Reed-Muench Cumulant Method.3 No other statistical analysis was performed. Location of Raw Data and Final Report: The raw data and a copy of the final report will be retained in the archives of HLA. PHONE (606) 241-4471 FACSIMILE (608) 241-7227 TELEX TLX 703956 HAZRAL MDS UD O O H N W O Laboratory S a rvcN Company 000818 .(fi) HAZLETON LABORATORIES A M E R C A fMC. 3301 KINSM AN BLVD., P.O. BO X 7545 MADISON, Wl 53707 U SA HLA Number: 91201739 Sample: T-4416 Page 5 SUMMARY OF RESULTS Test Animal: Albino Rats - Crl:CDBR Source: Charles River Laboratories, Inc., Portage MI Date Animals Received: 12/04/89, 12/18/89 and 01/08/90 Method of Administration: Oral Gavage Start Date (In-life): 12/28/89 End Date (In-life): 02/16/90 Estimated Oral LD50 : Male - 1.8 g/kg of Body Weight 95% Confidence Limits - 0.7 to 4.9 g/kg Female - 0.6 g/kg of Body Weight 95% Confidence Limits - 0.2 to 1.5 g/kg Sexes Combined - 1.0 g/kg of Body Weight 95% Confidence Limits - 0.4 to 2.4 g/kg Sex Male Female Dose Level __ (g/kg) 0.2 1.0 5.0 0.2 1.0 5.0 Average Body Weights fo1 Initial Day 7 Terminal 290 332 272 264 274 -- 362 315 -- 211 247 233 257 230 -- 254 268 -- Mortality (Number Dead/Number Dosed 1 0/5 1/5 5/5 0/5 4/5 5/5 PHONE (606) 241-4471 FACSIMILE (606) 241-7227 TELEX TLX 703956 HA2RAL MDS UD O O M N M O Laboratory S ifw c w Company 000819 (**) HAZLETON LABORATORES AM EHCANC 3301 KINSM AN BLVD., P.O. BO X 7545 M ADISON. Wl 53707 U SA HLA Number: 91201739 Sample: T-4416 Page 6 Observation Appeared normal Clinical Signs (Number of Animals Affected) nuui L lO Z A A 1 Z col 1 lfl i l 11 11 11 Hales i(Q -L -q /k a l 5 5 5 555555555 5 5 5 5 5 Appeared normal Females (0.2 Q/kq) 5 5 5 555555555 5 5 5 5 5 Males H .O Q/kq) Appeared normal Hypoactivity Red-stained face 5 5 5 521112333 3 3 3 4 4 0 0 0 032222111 0 0 0 0 0 0 0 0 0 12221111 1 1 0 0 0 Yellow-stained urogenital area 0 0 0 0 1 3 3 3 1 1 1 1 1 1 1 0 0 Ataxia 0 0 0 000001111 0 0 0 0 0 Thin appearance 0 0 0 000001111 1 0 0 0 0 Diarrhea 0 0 0 0000000 11 0 0 0 0 0 Death 0 0 0 0 10000000 0 0 0 0 0 Females (1.0 a/kg) Appeared normal 5 5 5 511111111 1 1 1 1 1 Hypoactivity 0 0 0 022 100000 0 0 0 0 0 Red-stained face 0 0 0 0 2 2 1 0 0 0 0 0 0 0 0 0 0 Ataxia 0 0 0 001000000 0 0 0 0 0 Prostration 0 0 0 000 100000 0 0 0 0 0 Death 0 0 0 0 12 100000 0 0 0 0 0 PHONE (608) 241-4471 FACSIMILE (60S) 241-7227 TELEX TLX 703956 HAZRAL MDS UD O O B W N O Laboratory Sotvicm Compony 000820 HAZLETON LABORATORIES AMEBCA, MC 3301 KIN SM AN BLVD., P.O. BO X 7545 M ADISON. Wl 53707 USA HLA Number: 91201739 Sample: T-4416 Page 7 Clinical Signs (Continued) (Number of Animals Affected) Observation Hour _____________________ D M ________________________ 1.0 2.5 4.0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 Males (5.0 o/kg) Appeared normal 1 0 00 Diarrhea 4 4 41 Hypoactivity 0 4 41 Red-stained face 0 2 4 1 Lacrimation 0 0 10 Ataxia 0 0 01 Death 0 0 1 4' * - One animal found dead after the morning observation. Females (5TQ q/kq) Appeared normal 2 0 00 Diarrhea 3 3 20 Hypoactivity 1 3 20 Lacrimation 0 2 20 Ataxia 0 1 10 Red-stained face 0 1 1 0 Death 0 2 12 PHONE (608) 241-4471 FACSIMILE (606) 241-7227 TELEX TLX 703956 HAZRAL MDS UD . OCRAHNO Uboc.wr, C*me.,w 000821 HAZLETON LABORATORIES A M E H C A .N C 3301 KINSM AN BLVD.. P.O. BO X 7545 MADISON. W l S3707 U SA HLA Number: 91201739 Sample: T-4416 INDIVIDUAL PATHOLOGY COMMENTS Dose Level: 0.2 g/kg of Body Weight Animal Test Dav Number Sex Pied Sacrificed NecroDSV Observatio C50366 M - 14 No visible lsions C50367 M - 14 No visible lesions C50363 M - 14 No visible lesions C50369 M - 14 No visible lesions C50370 M - 14 No visible lesions C50501 F - 14 No visible lesions C50502 F - 14 No visible lesions C50503 F - 14 No visible lesions C50504 F - 14 No visible lesions C50500 F . 14 No visible lesions Page 8 PHONE (608) 241-4471 FACSIMILE (608) 241-7227 TELEX TLX 703956 HAZRAL MDS UO OORIMIMO Laboratory S arvicw Company 000822 HAZLETON LABORATOH eSAM 6flCA.NC 3301 KINSM AN BLVD., P.O. BO X 7545 MADISON, W l 53707 U SA HLA Number: 91201739 Sample: T-4416 Page 9 INDIVIDUAL PATHOLOGY COMMENTS (continued) Dose Level: 1.0 g/kg of Body Weight Animal Test Dav Number Sex Died Sacrificed NecroDsv Observation C49861 M - 14 No visible lesions C49862 M - 14 No visible lesions C49863 M - 14 No visible lesions C49864 M 2 - Stomach, Glandular Mucosa: diffusely red C49860 M - 14 No visible lesions C49921 F 3 - Stomach, Glandular Mucosa: diffusely red C49917 F 2 - No visible lesions C49918 F 4 Perinasal Discharge: red Stomach, Glandular Mucosa: multiple dark areas, up to 2 x 4 mm C49919 F 3 - Stomach, Glandular Mucosa: diffusely red C49920 F - 14 No visible lesions PHONE (608) 241-4471 FACSIMILE (608) 241-7227 TELEX TLX 703956 HAZRAL MDS UD HAZLETON LABORATORIES A M ERC A , MC 3301 KIN SM AN BLVD., P.O. BO X 7545 M ADISON. Wl 53707 U SA HLA Number: 91201739 Sample: T-4416 Page 10 INDIVIDUAL PATHOLOGY COMMENTS (continued) Dose Level: 5.0 g/kg of Body Weight Animal Test Dav Number Sex Pied Sacrificed Necropsy Observation C49191 M 1 - No visible lesions C49192 M 1 - No visible lesions C49193 M 1 " Stomach, Glandular Mucosa; multiple brown areas, up to 3 x 1 mm C49194 M 0 - Stomach, Glandular Mucosa: diffusely dark red C49490 M 1 - No visible lesions C49131 F 0 - Stomach contents: clear white fluid C49132 F 1 - Stomach, Glandular Mucosa: multiple brown areas, up to 4 x 2 mm C49128 F 0 - Stomach Contents: clear white fluid C49129 F 0 - Stomach Contents: clear white fluid C49130 F 1 - No visible lesions PHONE (60S) 241-4471 FACSIMILE (608) 241-7227 TELEX TLX 703956 H AZR Al MDS UD O O P N jwQ Laboratory Sarvic-- Company 000824 HAZLETON LA BO RA TO RES AM ERGA.IM C. 3301 KIN SM AN BLVD.. P.O. BO X 7545 MADISON, W l 53707 U SA HLA Number: 91201739 Sample: T-4416 Page 11 REFERENCES 1. "Acute Oral Toxicity," Organisation for Economic Cooperation and Development's Guidelines for Testing of Chemicals, Section 401 (adopted May 12, 1981). 2. NIH Publication No. 86-23 (revised 1985). 3. Thakur, A. K. and Fezio, W. L., "A Computer Program for Estimating LD50 and its Confidence Limits Using a Modified Behrens-Reed-Muench Cumulant Method," Drug and Chemical Toxicology, 4(3):297-305 (1981). PHONE (60S) 241-4471 FACSIMILE (606) 241-7227 TELEX TLX 703956 HAZRAL MDS UD O O R N W O LaborM ary Sarwc-- Company (00825 HAZLETONLABORATORES AMERCA.VIC. 3301 KINSM AN BLVD.. P.O. BO X 7545 M ADISON. W l 53707 USA HLA Number: 91201739 Sample: T-4416 Page 12 PATHOLOGY REPORT There were 10 rats (5 males, 5 females) from each of 3 dose levels (0.2, 1.0, and 5.0 g/kg) necropsied. The animals that died on test (DOT) were refrigerated and necropsied within 24 hours, usually the same day. All surviving animals were euthanatized and necropsied at the termination of the study. The dose level, day of death, and gross observations recorded for each animal are on page 8 through 10 of this report. All animals in the 5.0 g/kg dose group died on test. At necropsy, the only changes observed were in the animals that died on test. The most frequently recorded observations pertained to the content and coloration changes in the mucosa of the stomach. These findings possibly represent test material mixed with ingesta, postmortem change, or both. All remaining observations in the DOTs were considered postmortem change, incidental, and unrelated to the test material. There were no visible lesions in the sacrificed animals. Pathologist (91201739.cjr) Date PHONE (608) 241-4471 FACSIMILE (608) 241-7227 TELEX TLX 703956 HAZRAL MDS UD * O O W N W O Laboratory Sorvtcoa Company 000826 HLA: ^ n o n 3 C1 PERSONNEL SIGNATURE SHEET ACUTE TOXICOLOGY Name Job Title Signature Initials Becky Beckwith Ann Bursaw Lab Animal Technician Sr. Clerk 'i.ak^-ffghuiLtJj /p7ioijE>OAjOassr' Anthony Cass Lab Animal Assistant D kA v,/Twq C o Jxa. Cindy J. Cary, DVM Lab Animal Veterinarian Kari Gassen Sr. Lab Animal Assistant j^oJLi Steven M. Glaza Section Supervisor Ben Haley Sr. Lab Animal Assistant Jeff Hicks Lab Animal Technician Sharen L. Howery Research Assistant Jim Jirschele Lab Animal Assistant J,L J L s A / V Wayne Madison Senior Section Supervisor C ()"? "t Q. oj Bud McDonald Eileen McConnell Sr. Lab Animal Assistant Administrative Clerk rtk*_P .-iX.r'x-/ Henry Mikula Albert Oleson Lab Animal Assistant Sr. Lab Animal Assistant M & u f Q u , r s A O __ Patricia Padgham Jane Pol now Steven R. Sorenson Dawn Thieding t Annette R. Turner '2> 1___ (09/11/89) - 000827 BEST COPY AVAILABLE DOSE AUI11HISIRATI0II/0DY UCIGtt/HORl ECORD HLA: g? U l S Study T t t ls l Acute OrI T o xicity Test M a te ria l; ~7~~- *4 *4 I Vellido; A A Bulk Density; ) , Dose le v e l; O . 2 _ - (a/ri-1 S pectes/Streln: Albino Rat/Snraque-Uaulcy Suurcoi C iarlos R iver Dele Received; / ~ 8 S O (g/kol Fested Date/T Ina/Taclinlcten 2. -I / 3 :o 5~ii -w-/ C lh l Root Ho. ) & '2 -- Route of A dm inistration; Orel Gavage Dose Volume 0,lC) (ad./kgl Sex: rileie^Fcieele Animel No. CS' o g c . Oli'l Pretested Body Weight (q) NA oyff Hose Tlau: S f)0Ar\ c\lo \ Tccluilc 1an (W iyyu Data V- Scale Used KTron HA v Fested llody Uelqlit (q) iH Actual Dose |a(L) Dev 1 Dody Uelqlit |q( o .o S IVl Dey 14 Dody Uelqlit (q) Deed Body Uelqlit (q) Dose Volume 0 . ( r i./lq ) Anlieel Ho. CS~' c S c I 12 ^ QoS ifi\ 0.0*5 o 3 T I 32 F y<* Sexl llalo/fcmaTc ) Of0~L o'Sol) c 5 c 4 2f 0 .0 `S JlT> IS* oS^o \ \ \ _v Dnse Tleio: 9- St/frr m ajj V? (W Y/c Technician iyyu Data V-u V e ril led by: a -2 - 9 0 Szz-K S~ZZX Scale Used KTron HA Pretested llody Uolqlit (q| Fested Body Uelqlit (q) Acluel Duse (ml) Day 7 llody Uelqlit (q) lljy 14 Uudy Uelqlit |q) Deed Undy Uelqht (q) NA -- \-- 1^4 zcf\ 21"? 2 OCe 0.01 r o 3 D.ol v . o 3 ZJY 2 l)I 23^ 1 1 > 1 4 C. 2 4 ( U L<*> I H 1 \ \ \ m V t_ (0 /-J W V*? ________ m . V /C i $7-1-8 V a rilla d by l 1-2-90 S'2 Zir S2JS- Dose 1evel Hiomurs ia^aL Sex 0. 2 cT 0L~ C.lr ? oTs lech. cA Tine NA Hat. 1990 & NA - Hot Appllceble HORTAITV (HO. PIEP/HO. DOSED) Tinly Hay m PH i% L L J7 -2L r* t?" %% wwfoAHr--IkIPH7AHAs--fZPsH%AlLLrrPL^miiA?ssr"PHmA toi r wft m W .V Hi & lie)cO-\C 1 >* 72 wy y.rr % lk .4 % fvi A Reviewed Dy ,7 7 / Deta .9-9.9 90 000828 BEST COPY AVAILABLE Dose leve O 1- OBSERVATIONS (Individual) Test material: ~~T- L-jL I r( HLA: 3 / 7 ^ 1 1 3 ? Surviving animals submitted for terminal necropsy. A , Technician V _____ Date Surviving animals designated for sacrifice and discard. Technician NA__________ Date NA_______________ Indicates condition exists SI Slight-. - Condition not evident. * found dead, P.H. check. NA Not applicable Reviewed by: J?/_____ Date: ________ 000829 BEST COPY AVAILABLE < Dose level: O .*2. OBSERVATIONS (Individual) ________________ _Test material: ) - I C-? (( HU: Q ILo in'}*? Surviving animals submitted for terminal necropsy. Technician Date 1 - I L ' ^ O Surviving animals designated for sacrifice and discard. Technician NA__________ Date NA_______________ Indicates condition exists SI Slight-. - Condition not evident. * Found dead, P.H. check. NA Not applicable Reviewed by: .s?/_____ Date: _______ S fa 000830 BEST COPY AVAILABLE DOSE AUtllHISIRATIOII/OOY WEIGIII/HORTA. f RECORD HLA: l l Z n f 1 3 7 Study T itle s Acute OrI T o xicity le s t H a te rla l; ~7~ - *-/ *4 I O ____________ Vehicle; N A ___________________ Bulk Density; 1 7 . S ' lg/L) Specles/StreIn: Albino Rat/Snraiiuo-Uawlcy Sunrco; Charles R iver Dele Received; l ~ L ' l Uote le v e l; / . O (a/kg) Fasted Date/Tlno/TechnlcIan I / 3 ~ n.ei. / R o o m Ho. (<0 1 Route of A dm inistration: Orel Gayago Uose Volume O '! B ( u l/ lq l A n im i Hu. " Prefested Body Weight Iq) NA Fisted Uody Weight Ig l UT Sex: fUTieiFemale a> % '\ 17^ n t 11 t 1 1 'b \Hose TImo: \ 2 > A " Actual Uose In i) Uay 1 Body Weight (q) Day H Body Weight (q) Dead Body Weight (g) Dose Volume 0 . 3 T Iml / lq ) 0 1 1 0 -1 | If* -/ 3^ 333 o il Z4Z 330 V 0 .1 1 n s* l- lt - f o AU 1 u o Z l< * Sex; lla lo /fcm a li ) \ v\ \ Dnse Timo: Animal Ho. /Y e ti sled Body Ualght (g| ^2 1 NA t in t ? / ? M l 1? 2 Technician W &< /J W fjJ Technician w 1990 Date A xh /? `L /v b 1990 Date '/ f Scale Used K T r o r HA S 2 -l Ver If led by: J * .V /-?-3C s S iz - ^ Scale Used K T ro n HA --V lis te d Body Weight (q) Actual Duse (ml) lit 0 . 1? 1W z \ o .n z \< o .n 2i f o - 1? \ \ (1 1 1 O bi '/ ? lA V e rll lad byi . P ? J'(-9 '-3 o Hay 1 Body Weight (q) Hay 14 Budy Weight (q) Head Bndy Weight (q) Q c*>d J h & H Fl - n O l- n - S a /- /j - r a 0,11 /V-l <*/ islL o Z Z ^ /? Q 2S 3 26^ m ________ Q L . 'M $ZX% $1X 2 000831 Reviewed Dy , 7 ? - / Pete -# > best copy available Dose level: 1 . 0 OBSERVATIONS (Individual) _________________Test material: / - Q-*4 I HLA: ^ Z C > n 2 9 Surviving animals submitted for terminal necropsy. , Technician Mr-/_____ Date Surviving animals designated for sacrifice and discard. Technician M __________ Date _NA_______________ Indicates condition exists SI Slight. - Condition not evident. * Found dead, P.M. check. NA Not applicable Reviewed by: *37/_____ Date: ________ -90________ 000832 BEST CPY AVAILABLE Dose level: / & OBSERVATIONS (Individual) _________________ Test material: ~ j ~~ *-/ 8 I C _______ ( HLA: C H I P n l & Surviving animals submitted for terminal necropsy. ^ , Technician Ur-i______ Date I -?3 Surviving animals designated for sacrifice and discard. Technician M __________ Date NA_______________ Indicates condition exists Si Slight. - Condition not evident. * Found dead, P.M. check. NA Not applicable Reviewed by: J 7 / _____ Date: ______ ____________ 000833 BEST COPY AVAILABLE f DOSE AUMiNISlftATlOfl/DCJUY WE IGIIT/MORIAL.. f RECORD HLA: 3 / Z O / -7 ..3 ? Study I I t la I Acut O n ! T o x ic ity Test H aterI 1i / - Lt-i4 l a > __________ V elitela: A /^ 1 ____________________ Bulk Density: /. ~L% (g/wLl Speclea/Stralit: Albino Rat/Siiraquc-awlcy Sunrco: Charles H lvor Date Received: / 2 * 4 ' ? ^ Dos Level: O la /k o l Fasted Data/Tlaio/Teclinlclan /2 - 2 7 S f f i/ 2 0 /~ i Room Ho. / 7 ~ Z - Houta of Adielnlstret Ion: Orel Cavano Uose Volmac \ - ) l I at /k 9 1 Animi No. _____c A i Sex: f iiiie } F cimi Scale Used KTrOPj e x fo t' 1 fi/4 /i lS-SV c_*--i (ITS S tX* & tO . Dead Body Height (q) Dose Volume "5 ^ / (m l./tq) Animal Ho. CA ' Prcfastcd Doily Uolglit In) NA Lasted Uody Weight (q) Actual Duse (mL) Hay 1 llody Helqlit (q) Hay H Uudy Weight (q) * of\^ Head Umiy Weight (q) Sex: llalo/Fcmali ) Dosa TUio:0'1"LAt^ *\iVL < \ i W ` l i o \ --\ V i t o V l P \ V 7? ^ V \ ? 0.<6% ()clJ n-n Z\% O.^o P*J oA o.*) Z p ^ J G)<?*gA n-ts-rt 0/4 \ \ \ \ Tedili le Ian w an Ql* Data '%r Scale UsedKTron HA --- =v Ver II led by: 'Vi-Sr J-/T-/ ij.m -Fj -C*O 00083 Uosa Hours Stud Hay 1evel im l 3 1 S E 7 B 9 I 11 12 13 11 Total (g/kgl Sex AM PH AM PM n r npit AM PH AH-- PII AM HI ait in AM PH AM I'll Wt I'll ah m AH I'll Air PH All PH 6 - 0 cf ' k if if s~.o ? MA HA HA % lech. q J CP HA a d Time NA n r % HA /V/1 iute 1? 'ja % ,HA 'li HA - Hot Applicable Reviewed Dy S ? / Data BEST COPY AVAILABLE Dose level: C> OBSERVATIONS {Individual) Test material : / - *-1*4 I HLA: U l & m i y lo A<~ m iz z<? ^ 9 - Surviving animals submitted for terminal necropsy. . Technician N Date A)ft_________ - Surviving animals designated for sacrifice and discard. Technician NA__________ Date NA JbOO Indicates condition exists SI Slight. - Condition not evident. Cfl * Found dead, P.H. check. NA Not applicable Reviewed by: j?/_____ Date: ___ Oose level: <5**. O BEST COPY AVAILABLE OBSERVATIONS (Individual) Test material: ~7~- *V /C h i.ft: '7 3 * 7 Surviving animals submitted for terminal necropsy. Technician _ Date A)f\ Surviving animals designated for sacrifice and discard. Technician MA__________ Date NA_______________ Indicates condition exists SI Slight. - Condition not evident. * Found dead, P.H. check. HA Not applicable Reviewed by: -7 7 / D a t e : _________