Document 82Jw8JpYB25Yg2ekMEN9JYLXZ

MZTtTX i An St. Lava 10. Mo. JHury 1), iy$lt /. -- -a CERTIFICATE OF ANALYSIS OBJKT - (A) Tta Aaut* Oral Toulsity (La,,) af ranatlaaal nail OB-SS Nr laU fB) Inhalation f 08-r.u hamw By Bobbitt 0*ar ParlaB of Thraa Daya (0) Oaular Irritation Prodaaoa Ty QB-SU mi oaranrn n* - IkoaN IMal Oaaoaay, lx. InU, Kawl Bbaaoato Praia*! To. i BA-10 (A) lb* IB*, aa Batorttaad by ta lntnAatta of < ilali Owa r wBllato* OB-tt Uto tta IMMO af %rna Malay attain alblaa rott by aaa of rttbor aathotor atI otto4 to a hppaOanV s fyrlto* lata otto o wlfM rua af 17S-Jk5 aaa wrt oa*B la tta toll. Aftor tta oppraslaato Mai aw lottol Bow aw faona, moo* of aalaala an SOB a loaolo talpM to bl --tot tta toatalty roaoa ttaraby awol/lac Mi k Mloalakloo of to Upa ttltt aw Moo aaoaroiaa to a oaBlflaatlaa af tta MM af ttlaa (Baart. J. af Kara, ai fbaraaoii. U, IT*). BaloOtoO wlaala fNo ttaaa raaalTtny a lathal Oaaa an gala* il* wo oaaraaaapl* MMtaoUoa aaW af Ito rloaart. Iha Oata aaa faanA la Tttla I. la a aauu attOn woroalaatoly i| aril fto* la 01 awtor attt * aloor plalla Onr far iliaaiiallw laalwA ttlto rabbit* owa plaaaB laalOa tta itantir ol *w***0 to na atottaa* at woo towittm Mr fr*la aaapraaaag olr tWiagt of tta Halo. A bom* OboI of rrattina took plaoa otto ooly a of M partial** api'lHa lo tta obaabar. Aa air pwoi af look aw aoaO. Iba rtthtta aoia owM % of BUaaafwrt. mbw tta air *>A allllMBB M 11tor. It or* fkr foor War* Ott aa oati i of atoMaaB 0B-5k laalto tta 1 BaAOaO to gl oo* a itoaw atoal * flalO fr*a a karatto aato a Hu 0B-5k aaa tolatlllaaO at * lawratora *f 1*0* Mb* 0. oAtt air lobab* at a rato af 1.0 lltor par aloato. DtoOor ttooa oaaOltAaaa a *aw< ttBttok w fa wa praBrail that abaaarad tta wlaala aigaptlnf ahaa ttay on aaa- tta trwoparao* t hw hi m* * m * i HONS 038050 MltMU . , . u* -bbwbcBSu** . bj *+*+*' * mmmtfCU rnmmmm dfcoadA* klMHIU IlMilMH 0crtlflaata of Analyala -- fifi > (Uiy-w) ot tear. Tha rabbit* wn oxpoaad daily for tftra# '*ouilva day* for a total txpoaura tlua af 70 hour*. Tiw lurrlvora uara .-] 7 day* for observation a/tar oosplatlor: of tha axpoaur*. Six young natura albino rata uara Io pi wad In tM draw alontslda lha rabbits Uw iaat day of aposor*. Tha aonoantration of vapors vaa found by passing a mu rad voluna of ehwnar funaa through a train of tnr*# absorption Haaka ooRtalnlng aaatoaa and tha optlaal danalty road on a Baokran Spaatrophotonatar at a mn langta aoitlng of 750 adlUnloron*. Tha aoatona was firat slauly avaporatad aff d tha raatdua takan up in athyl aioono], Tha oonoantraV.on par litar of air ana found by ooapariaon wltn a aurra praparad fron known awwita af 0d-5ii diaaolvad `.n athyl alaohol. Tha data ara givon in Tablaa II and III. (C) Tha dagraa of aaular Irritation produoad by 09-S* was found 97 uaing tna tattailqua drralopad by Drtlia, doodard and Calvary (J. Pharuaaologr and i>parlaant&l Tharapautiaa, Pal. 62, Ha. ii, lttii*)* Brlafly, It sonaiatad of dropping 0.1 ^UlllUr af taa fluid law Wo oonjunotlval aaa of tna right aya nf lv alMiw* rdbtita aad afeaarrtn* tha affhat avur a parlad af aaaaraJ day*. Hm la ft eja aarvad aa a aantrol. Tha aaoraa ara flvan in Tatola IV. MONS 036051 MaUlU L*H OarUflaM. ( iMlyMa -- ran J (l-iJ-S.) TABLE I THE ACWT* ORAL 10110111 (LD, ) OF nucTToujo. nr.nn os-5i* nm rats Animal ho.* So* 1- Halt ?- HU. 3- Faaal. A- rtmMlt S- Hal. 0* Hal. 7- Bala 4- I'muO. 9- Faud. L0- Fond. 11- KaLa 12* Halt 13- Hal. 1A- Hal. 15- FMwl. 16- Fm1. 17- Fm1. ia- Hal* IF- Hal. tO- Pnwl. a- Null It- M. 3- FM<1. a- Hai* tj- hai. 16- Hal. 77- TMili ?#- Ptaala IF- Halt 30- Hal. 31- Hal. Ji- Nai. a- mu 3A- taila 9- *Ml< 36- FmmI. 37- MmI. 36- Hal. 3- Hal. A0- Hal. Al- Hal. At- Hal. A3- Fraal. Lit- Ftmala MUht [iron* 2)5 no 190 175 2w0 2)0 2A? 200 lb? 205 220 21*0 210 225 10C 195 175 220 2i*0 200 190 230 210 2b5 225 230 15 2"5 230 215 2?5 2ii0 2U0 ldO 200 195 195 225 220 210 235 235 TOC I8e Oooo C./K(. 9*07 12.10 15.10 10.15 Poto Swlomd Rod 9nrl*d 5rrlnd Durrlvod Dlod Surrlvod *arrlod Surrlvod finlnd SurvlTod ntod mod forrlood SurrlTod SurrlTod Dlod dwrlrod Jurrlood Surrlomd Dlod Surrlood UTTlTOd mod Surrlood m*d svnvfd mod m#d DUd Swrlood Dlod mod mod Dm iliod Dorolood DUd DUd Udud DUd Dlod mod HONS 038052 * i kllogm nith loaar and appor llsdta of ran par nia|iaa arrival Um for Umii aniaala raaairint a lathal daaa varlad trm Jd hoar* to d aaya. Thar* m aodarat* to araar* dlarrhoo ia all <--> doaad at tha la**}* 9fm la Uia tabla. hmon loot port af tha Mr aa tha uodaraida of thair bodias. Howaoar, tha hair wmw haak aftar aovaral aaaka. At Mtopay of anlaalt raaoirlnf a iathal doaa, tha tntoatlnal traal ana (aaaoua and IrrUatad, and both tha llvar aod aploon vara afcaoroally dark. Mill 71 PATS AJflD tKXOHT dUMM OT RABBITS BCraD TO QS-SU Tiras M1H ha.- Sox X* HiOa * Nda J- Ma A- Halo Initial Haight lUggraos 1.0 l.S 1.1 1.1 Haight Cbaaga 7 Bajra Altar tegaatra Mprsaaad Aa * af Initial IM|H 0.0 -- ~ rata OUd--2nd Day arvlaod Uad--2nd Dap Dl*d--3rtl Da? TAILS nz ATOMS WOT OOSGBlTSATXflS OP m-fk m za cuamb Oonoaniratla* of os-5k Vapors Hi.Altar Rvarapi Asoraga alatlw HBdAltr Uoiaa flka*a* t HnA Imm Mat MO MO i-n 0.0 ai.s r.j SM *0.5 A- Aoaraga M* *. 4 i i HONS 038053 V WMitt krtinMi at Mfrii 1 (1-U-A) nMMSiai - Tha four rabbita nnln< tha firat day'a iipoim althaagh thay aaru ratbar aihamtad uhao tain* rat of tha ahaafcar. Tho fuaa proton* aadarataly ooooio UMl lrrltatlra that aauaad tka anlaala to oaorah 00007 aarnar of too too la an offort to aaaapo tbaa. Ihirlni tka aooond tap maa taa of Ua oalaala glad, a oaaal dlaaharga, aenalatlng of thlok poiloaUb aaaoa, ippaina. it tka aad of tha aooond dap, bronahlal raloa oaro amdaat la tka too aakiloara. katk laaaoarad raaaonably mil ooornlght but ona dlod darlnc tka third dap*a aapoaara. Tha anrrloor ragainad lta naraal anpotlta within a tan dap* and at tka and af tha 7 day* obaarratlon porlod appoarod to bo In aatiafaotorj aaadltira. Tha fraaa oauaod aodarato ooular arjthaaa wltb adld adaaa. Laarlaatlon am aodarata but altaroa ap aoon aftor tha aalaala waro takan out of tha drur oaah day. Tha ala rata a^oaad tar approjdoatoly aawon houra to tha oapava awoload fll bo at laaldaat althaagh tha daproa of naaal and ooular irritation oaa ooraardnlo to that far tha rabhlta. Tha aoarogo aaaamtratlaa of QS-$lt foil wao 2.69 adlllgraa* par lltar af air at an ororofo upiarawio af 6U.6* 7, and rolatioo kadtlt; of 59.SE. %prartnad*lp Ml af tha flald oaa oalatillaad mdor tho oondltlana af tha Uat. haoroaaapla oxanlnatlan at antepay of rabbdta dying in tha ahanbor roooalad ooworo pal nonary aragootlon with maaorma honorrhawla arooa proaoat. t 11 l i n SOCKS HPM3MTIIB Tt* SirttlTT OP oonjn imiTurioh fwwocd n rancnam puns os-Sk tolaal no. 1 t i 6 * -- 2k 16 *tt 3k to tt.7 mmiau. niuJiTioh at the no or h Bouro 2k Hanra k6 Houra T2 Houro 26 72 7k 22 28 20 *3.T 11 16 11 16 22 12 IT.* S S 10 10 10 6 i.6 0 0 0 0 0 0 0*0 hons 03*056 & tMrtltlula of dnaijan - Pag. (l-lXk) J Doamva Oaular Irritation oonalatod of noda rata arplhaaa with alight la nt Oaraaa adaow 4aaloping within tha flrat hoar for an awaraga aaara of tt7 oat of a poaalbla aaora of 110. Tha Iris and aaraaa aara aolf allghtlp affaalad althoagh thojr aara aooaahat Kail Ihr aoaaral hoara haaanaa af llaa aaatlag af flald. KOaaa aaa aonfinoa prlnolpallp to tha ajalldB. tha aaaat had laaaiad to 17.0 aftar 2li noura and ail alma of Irrltatiaa had Haappoarad idtala 77 haura. summit - (A) Tla houta Oral Tojdalty of haatloaal (laid 0d-5b for rata aaa found la ha U.S0 grata par kilograta alth fiaaalal UMta af U.hO-liiJiJ |rta par kUagran. (I) Mar rahblta auralaad aaa dap*a aapoaara ha 00-Si faaaa at a aaaaantratiaa f (.10 aonigrtaa par Utor af air. Taa af tha aalaala Had ahrlog tha ttaaad Ifa aapa aara aMla a third Hod da lug tha third dap. Tha naat aara raaaar daaoa la tha ahaabor. A |Mp af rata txpaaad for ona dap aadar tha aha ooaiHtloaa aaa alaod aithaat It wm ooaoladad that tB-9k fiaao ora roaonaablp toaio la haaap aaaaoatratiaos wr aa aatoadad parlad af a^aaara. (0) M aaa adld ta aadaratalp Irritating to tha apoa af rahblta. da aaavoga aadMi aaara af ().T oat of a paoalhlo no oao itiarHd b Mat aftar tha flald aaa plaaad la tha apaa. J ICHhTITTC MdOCXlTB it i ran *. rowon -'i i HONS 038055