Document 7ZZDoJEj0NRjY1d9akpeo58g

FOR DU PONT USE ONLY AR226-2944 Du Pont HLR 444-89 Study Title Static Acute 96-Hour LC50a o Fathead Minnows (Plmephale to Author David G. Hutton Study Completed On December 5, 1989 Performing Laboratory E. I. du Pont de Nemours and Company, Inc. Haskell Laboratory for Toxicology and Industrial Medicine Elkton Road, P. 0. Box 50 Newark, Delaware 19714 Laboratory Project ID Haskell Laboratory Report No. 444-89 # Page 1 of 9 Company Sanitized. Does not contain T S C A CBl Du Pont HLR 444-89 GOOD LABORATORY PRACTICE STATEMENT This study was conducted according to EPA Good Laboratory Practice Regulations (4P CFR 792). Any areas of noncompliance are documented in the study r ords. No deviations existed that significantly affected the validity of the study. Submitter: E. I. du Pont de Nemours and Company, Inc. Sponsor: Chemicals and Pigments Department E. I. du Pont de Nemours and Company, Inc. Study Director: 9l(6>l2T David G. Hutton Consultant Environmental Sciences - 2- Company Sanitized. Does not contain T S C A CBI 4 Du Pont HLR 444-89 GENERAL INFORMATION Material Tested: Medical Research No.: Haskell No.: Purity: Synonyms: Other Code: Sponsor: Material Submitted By: Study Initiated - Completed: Notebooks : There are 9 pages Distribution: Chemicals and Pigments Department E. I. du Pont de Nemours and Company, Inc. Wilmington, Delaware Chemicals and Pigments Department E. I. du Pont de Nemours and Company, Inc. Jackson Laboratory Test initiated: Test completed: Study completed: 7/14/89 7/18/89 7/26/89 Company Sanitized. Does not conainTSCA CB1 Du Pont HLR 444-89 Title of Report Static Acute 96-Hour LC50a o f P 0 | H U Fathead Minnows fPimephales promelas) Summary ^ ^ ^ ^ ^ - 1 7 , 9 3 1 ) exhibited no significant acute toxicity to fathead m S w H p i m e p h a l e s promelas), a warm freshwater fish, under ^ j Jserate test conditions T O n g a 96-hour exposure ?n ||ch of greater than 4000 mg/L, because only 5 percent mortality occurred in each ot the 2700 and 400C mg/L concentrations, the highest concentrations Obvious insolubility was observed at all test concentrations from 800 to 4000 mg/L. Reported by: Study Director: . f/ W David G. Hutton Consultant Environmental Sciences & v Approved by: Frank X. Phillips, Ph.D. Manager Environmental Toxicology and Information Services Division ln /is Mb/Day/Vr // JQAfi Mo/Day/Yr Reviewed and Approved for Issue: David G. Hutton Study Director vy, J T J S ? Mo/Day/Yr DLT/DGH/FXP/smk/AQRP33.5 - 4- Company SanTfized. Does nol eonlain'TSCA CB1 Du Pont BLR 444-89 fillAT.TTY ASSURANCE DOCUMENTATION STUDY! H# 17,931 Static Acute 96-Hour LC50 o f ^ P P to Fathead Minnows (Pimephales proaeias) AUDITS: Items Audited Conduct Protocol, records, and final report Audit Dates 7/14/89 8/28-29/89 SHORT-TERM AUDIT REPORT NUMBER! ------DATE FINDINGS REPORTED TO MANAGEMENT AND ______ STUDY DIRECTOR: 8/29/89 Reported by: -- . --- William J. Lynam Quality Assurance Auditor D ate - 5- Company Sanitized. Does not contain T S C A CBI Du Pont HLR 444-89 In+roductlon/Purpose To determine the static acute toxicity o l j p i ^ H I (H-17,931) to fathead minnows (Pimephales promelas). a warm freshwater fish. Materials/Methods (Aquatic S0P-T02) The test material * H H V was placed into 5-liter aquaria, and diluted with laboratory well watt"' :3 yield the desired nominal exposure concentrations in 4 liter Ha l volunes (11.5-cm liquid depth). Two identical vessels, both cootaining only laboratory well water, were designated as the H^O controls. Chemical stability of the test and control materials in well water was not determined. Juvenile, unsexed fathead minnows (Pimephales promelas) from Haskell-bred stock with a 1.9 cm mean standard length (range 1.2 to 2.5 cm) and 0.079 g mean wet weight (range 0.020 to 0.194 g) were randomly placed in the test vessels, 10 fish per test vessel, two test vessels per concentration. Loading was 0.20 g fish/L. Fathead minnows are identified by labeling the culture and test vessels. The fish were maintained at Haskell Laboratory for approximately 47-53 days, with the last 21 days in a 272-liter Fiberglas tank (84-cm in diameter by about 49-cm liquid depth) before being used for this test. Holding temperature was within 5C of testing temperature throughout the holding period. The dissolved oxygen concentration during acclimatization was assumed to be at or near saturation because air was continuously bubbled through the water. No treatment of the fish for diseases was required during the holding period. Fish were not fed for ^ approximately 48 hours prior to nor during the exposure. The test solutions were not aerated, and temperature was maintained between 21.8C and 22.2C. Photoperiod was maintained at 16 hours 1ight:8 hours dark. Mortality counts and observations were made approximately every 24 hours during the 96-hour exposure period. Dissolved oxygen and pH were measured in the H20 control and in the low, medium, and high test concentrations at the beginning of the test and at approximately 24-hour intervals during the 96-hour exposure period. Total alkalinity, hardness (EDTA), and conductivity were measured at the beginning of the test in the H20 control. Records All records from this test and the final report will be maintained at Haskell Laboratory for Toxicology and Industrial Medicine (Newark, Delaware) or archived at the Du Pont Records Management Center (Wilmington, Delaware). - 6- Company Sanitized. Does not contain T S C A C BI Du Pont HLR 444-89 Results Test results for fathead minnows exposed t o 0 H H } ( H > 1 7 , 9 3 1 ) are presented In Table 1. The 9$-hour LC50 was >4000 mg/L, because there was only 5% mortality each 1n the 2700 and 4000 mg/L concentrations, the highest concentrations tested. All test concentrations from 800 to 4000 mg/L remained very milky throughout the test, to the point where the fish were hard to see. The chemical and physical parameters measured during the test of f l H H h are reported 1n Table 2. All parameters for this study were within acceptable ranges. Dlscusslon/Concluslon a has been tested for acute toxicity with fathead minnows (Pimephales , a warm freshwater fish. Under the conditions of this test, was found to exhibit no significant acute toxicity. In a rangefinding test at nominal test concentrations of 0.1, 1.0, 10, 100 and 1000 mg/L, 20* mortality was observed at 24 hours in the 1000 mg/L concentration. Footnote aLC50 = That concentration which 1s lethal to 50* of a test population during the specified time period. Company Sanitized. Does no! contain T S C A CBI Du Pont HLR 444-89 TABLE 1 RESULTS OF A 96-HOUR STATIC ACUTE TOXICITY TEST, WITH FATHEAD MINNOWS ( P 1 m e o h a 1 ^ ^ | ^ y E X P 0 S D TUj >(H-17,931) Nominal Test Conni icMeni ittrMaftfiAoint es (n9/L)_____ HgO Control 800 1200 1800 2700 4000 nrCumulative Observed Mortality ?2d4 HHrr.. j* 48 Hr.. B* 7722 HHrr i- B* (t)_____ 96 Hr A* 00 00 00 00 10 0 10 0 00 00 00 00 00 00 00 00 10 0 10 0 10 0 10 0 00 00 00 00 10 0 10 0 Replicate exposure chambers containing 10 fish per chamber 8* Company Sanitized. Does not contain T SC A CB1 Du Pont HLR 444-89 TABLE 2 RESULTS OF PHYSICAL AND CHEMICAL PARAMETERS MEASURED DURING A 96-HOUR STATIC ACUTE TOXICITjyEST WITH FATHEAD MINNOWS EXPOSED (Plmgphales promelas) M, Mil r j ical/Chemieal raidmeters Nominal T< t concentrations (mg/L) ---- 1800----- 800 " H-0 (High) (Medium) (Low) _ (Conti Dissolved Oxygen (mg/L) 0 Hr. 24 Hr. 48 Hr. 72 Hr. 96 Hr. 8,7 8,1 7,3 6,3 6,1 8.7 8.8 8.7 8.0 8.1 8.1 7.5 7.7 7.7 7.1 7.4 7.6 7.0 7.3 7.5 S 0 Hr. 24 Hr. 48 Hr. ' 72 Hr. 96 Hr. 7,5 7.5 7.5 7.5 7,7 7.7 7.7 7.7 7,7 7.7 7.7 7.7 7.4 7.4 7.4 7.4 7.5 7.5 7.5 7.5 Water Chemistry on the H-0 Control at 0 Hours Total Alkalinity fmq/L as CaC03 ) 69 EDTA Hardness (mg/L as CaCOjl 68 . Conductivit (umhos/cm) 170 -9- Company Sanitized. Does not contain T SC A C B t