Document 7RL7yMe14d94EKeVV8EVY6aBa

. AR226-OloYy FE DIETARY ACUTE NORTHERN BOBWHITE STUDY TEST SUBSTANCE Identity: Perfluorooctanesulfonate; may also be referred to as PFOS or FC-95. (1-Octanesulfonic acid, 1,1,2.2,3,3,4,4,5,5,6,6.7.7.8,8,8heptadecafluoro-, potassium salt, CAS# 2795-39-3) Remarks field: The test substance is a white powder. Sample was taken from 3M lot number 217. Sample was stored under ambient conditions prior to testing. Purity determined to be 90.49% by LC/MS, H- HMR, "F-NMR and elemental analyses techniques. METHOD _ Method: OPPTS 850.2200, OECD 205, and FIFRA Subdivision E. Section 712 Type: Dietary acute GLP: Yes Year completed: 1999 (Test), 2000 (Report) Species: Colinus virginianus Supplier: Wildife International Ltd. Production Flock, Easton, Maryland Analytical monitoring: Test substance concentration in standards and samples were determined by reversed-phase HPLC and mass spectroscopy. PFOS measured on Day 0 for homogeneity in feed and verification, and Day 5 for stability. Test phases: Acclimation -- 10 days Exposure - 5 days Past-exposure observation 3 or 17 days Statistical methods: LCso values calculated by probit analysis using the computer software of C.E. Stephan. Body weight data were compared by Dunnett's test using TOXSTAT software." No statistical analyses were applied to feed consumption data. Test bird age: 10 days Pretreatment: None Test conditions: Housing and environmental conditions: Indoors in batteries of thermostatically controlled brooding pens. Each pen's floor space measured approximately 72 X 90 cm. Ceiling height was approximately 23 cm. Extemal walls, ceilings and floors were constructed of galvanized steel wire and sheeting. Identification: Each group of birds identified by pen number and test concentration. Individuals identified by leg bands. 001364 Number of replicates: Six for controls, two for each treatment gNruomubperof bobwhite per replicate: five Number of concentrations: seven plus a negative control Feed and water: Game bird ration formulated as below, water from the town of Easton public water supply. Both provided ad libitum during acclimation and testing. [C`rCGoamceaBirdwRoateisonem [Soeysasarmesaaspen Tw msn| |wtwes]| [RovaySpec 80%Prem |_1%0 | p[vComeat _o2ws]| E[-- WetE arneProvacUs [0%| [OCFemFamaey oss | a aE Goes Tae) Vitamin and Mineral Premix [oC an --O | pam | [apmrionheeseRes | oroweme || C--O -- -- [[oVgreWeeraons Srp Vee) 58ogaermms || foE am ww] wee | [Eo oCde ---- r1] sTeegamm || 001365 Prophylaxis: None Brooding compartment mean temperature: 38 + 2C Ambient room mean temperature: 27.3 + 1.2C Average relative humidity: 31 + 14% Photoperiod: Sixteen hours light per day Lighting: fluorescent lights which closely approximate noon-day sunlight; averageof approximately 139 lux of ilumination Test diet preparation: Test substance mixed directly into the ration by means ofa Hobart mixer. No carrier was used. Diet sampling: Homogeneity of the test substance in the diet evaluated by collecting six samples from the 18.3 ppm concentration and six from the 1171 ppm concentration. Samples collected from the top, middle, and bottom of the left and right sections of the mixing vessel. These samples also served as the verification samples for these concentrations. Verification samples of the other treatment groups (two samples from each) and the control (one sample) were collected at preparation on Day 0. Stability samples were collected at the end of the exposure period (Day 5) from the control (one sample) and each treatment group (two samples each). RESULTS Nominal concentrations: Bk control, 18.3, 36.6, 73.2, 146, 293, 586, and 1171 ppm Measured concentrations: <LOQ, 19.5, 40.2, 74.5, 174, 291, 537, and 1196 ppm Element value: Dietary LCs) = 220 (164 -- 289) ppm No mortality concentration = 73.2 ppm NOEC (body weight gain) = 73.2 ppm All element values based on nominal concentrations Analytical Methodology: Diet samples were extracted with methanol. Analysesoftest solutions were performed at Wildife Intemational Ltd. using high performance liquid chromatography with mass spectrometric detection (HPLC/MS). When determining the concentrationof the test substance in the test solutions, the same and most prominent peak response for perfluorooctanesulfonate was used. No attempt was made to quantify on the basis of individual isomeric components. The LOQ (limit of quantitation) was 1.15 ppm in this study. The mean percent recovery of `matrix fortifications analyzed concurrently during sample analysis was 94.7. Samples collected for determination of homogeneity in diet ranged from 102-107% of nominal. Samples collected for verification in diet had measured values from 82 to 119% of nominal. Measured values for 001366 ambient stability samples taken at Day 5 ranged from 101-122% of nominal. Summary of analytical chemistry data: Homogeneity in Avian Diet EE=] rate |o2 les, [ov|mmeemmerms Rr] S-- E=I sessfsmension) Verification in Avian Diet owsi=ihee, r[HeS eo] [|] Bi I Te mMmi we CCom1m w=eee w]we Ambient Stability in Avian Diet oSliL,|sessfcS orto oi der,||oeln [etmewe] mem] me [=T=] rr=1 = m= w [=e1eTM]] 001367 Biological observations Mortalities and clinical observations: One incidental mortality occurred in the control group as a result ofa broken leg on the moming of Day 5. It was subsequently euthanized on Day 6. Two other birds in the control group were intermittently noted with foot lesions associated with cage mate aggression. Otherwise, all control birds were observed to be normal in appearance and behavior throughout the test. The first treatment-related mortalities occurred on Day 3 in the 586 and 1171 ppm treatment groups. Mortality occurred through Day 8 in all dose groups > 146 ppm with some of the deaths being during the post-exposure period. There were no treatment-related mortalities or overt signs of toxicity at concentrations < 73.2 ppm. There was 11% mortality in the 146 ppm treatment group, and two additional birds displayed clinical signs of toxicity (wing droop). All other birds in this test group displayed normal appearance and behavior for the duration of the test. Recovery with normal appearance and behavior occurred on Day 9 to test termination. There was 80% mortality (occurring on Days 5,6, and 7) for birds in the 293 ppm treatment group. Signs of toxicity observed prior to death included a ruffled appearance, reduced reaction to stimuli (sound and motion), lethargy, wing droop, loss of coordination, lower limb weakness and convulsions. Recovery with normal appearance and behavior occurred on Day9 to lest termination. There was 100% mortality (occurring from Day 3 through Day 7) for birds in the 586 ppm treatment group. Signs of toxicity observed prior to death included a ruffled appearance, reduced reaction to stimuli (sound and motion), lethargy, depression, wing droop, loss of coordination, lower limb weakness, lower limb rigidity, prostrate posture, and convulsions. `There was 100% mortality (occurring from Day 2 (noted on Day 3 for Day 2 aftemoon) through Day 4) for birds in the 1171 ppm treatment group. Signsof toxicity observed prior to death included a ruffled appearance, reduced reaction to stimuli (sound and motion), lethargy, depression, wing droop, loss of coordination, lower limb weakness, and lower limb rigidity. 001368 Body weight gain: When compared to the control group, there `were no apparent treatment related effects on body weight among the birds in concentrations < 73.2 ppm. During Days 0-5, statistically significant reductions in body weight gain or body weight loss occurred in the 146, 283, and 586 ppm treatment groups. Body weight effects could not be determined for test organisms in the 1171 ppm group due to total mortality. Feed Consumption: No apparent treatment related effects were noted for feed consumption for birds in concentrations <146 ppm. Reduced feed consumption was noted for birds in treafment groups > 293 ppm from Days 0-5. No treatment-related effects on feed Consumption in any of the surviving treatment groups during the Day 6-8 post-exposure period were observed. Gross Necropsy: All birds that died during the study, half of those surviving at Day 8 and the rest at test termination were subjected to a gross necropsy. Necropsy ress for birds found dead were similar, including thin condition, loss of muscle mass, altered spleen color, autolysis of tissues and pale organs. These necropsy findings were considered to be treatment related. The single bird euthanized from the 293 ppm treatment was found to have treatment related necropsy findings. Necropsy results for all other birds euthanized on Day 8 and Day 22 were unremarkable. % Cumulative Mortal |Concpenpirmatir foConterol ]e[eloTele[o]ele] [2| oo |olololololols] [me ooo olool lol] [wo[olowlolololwlw] [mm[oo[oololwlwle] [oo[0|olwlofololwlw] [NoG mnoralTes GEocurred|Tn aroy of ielTeai0ment a|vers fw omDayw 8 0 Da] y 22 wlwlw] "Bird euthanized on day3 after sustaining abroken eg 001369 BodyWeight (grams) [] J---- [---- Nominal_{MeanBody[MeanBody[MeanBod feanBocy{vieanBody]teanTalalIh| seanBody ConceGntermat)ion]| WDeiagyhto, || CWheainghgte|| CWheainghgte|| CWheaingghe | C(hWeaingghto~|fScohdanygWe,eiDgayh|] WDeaiygh2t2. 0a05| Dayss| Dayets[vayi522] 2: CNoengtariovle 2 || [wo[a Tow Tolalwl oo] [we[a[wwlawlowl ol wl] [me[ow Jololawlo olwl wn] [we [alolwlalalon] IE IP I IP I I [ew [=[TTTTT1 boyet:he aacrtbtehrasomlayyornoelb33dd mraenmuaailnleyd unatThaotrroausnidmianngt. gVraoulpuaefTstorr2D0a3ypo5r realmant grad ""SStiaaiissticcaallyyddiiffeerreennttffrroommtthheeccoonnilrollggrroouuppaalpp<=0<.00,501(ODumunentnrseLeesst)t. (-)=Nodata avaiable dus to morally. Mean Average Feed Consumption CoNnocmenitnraatlion, [FeeGda/BmrsdDa|y[FeedG/rBarmasDa|yFeedGEriarmdsDay||FeeGdrBarmasiay| pom Days05" | Days6s | Dayssis |Days sz losoecowsl 0 | 0| 0 |w| [ws [0[vw |w |w| oe [0[0|w[ w| me [0[0 |w [ w| Lowe[0[ow [uw [w| reTrpacied ow [| oo[ww|. |. 1] Low | . [ [o] T= No data avaiabe dus 1 moray. 001370 Gross Pathological Observations from Birds that Died in Study Tia Female, a, ndUndetermined Finding "CNoeretr N=2 N=8N=g1e0N=10 A`Abbddoommiinnaall ccaavviyy,, sauoimlayaiustohlryosuisghout EmCarocpi,aetmepdiy GFrlacTirraecdt elomgpty GHiezazra,rdanctoenrtioerntpsobrlloenstmaoitnleedd whitecoer HInotaerst,inpaallceontents tare KKieien,eypsr.ompialneent LLiovsesropflmeusacnlde mmoatslsed MSumsaciulinarsstkaareela, pale SSSpppeeaeennn,., gdbraaercykk SSpplieceenn,, pgarloey-brown TSShppiiiccneenn., ssmmaall and pale Not remarkable 0oo0 20 21 41 o oo o 22 i010 55 0 802 o oo o 21 51 01 o 0 o0 10 02 01 00 00 10 3z 0 0o 01 00 20 0 o0 o1 40 07 90 00 00 30 01 00 00 o0 o0 o1 02 oooo 01 0o 11 oooo 00 03 01 oo _ooo1 a0 20 CONCLUSIONS The dietary LC50 value for Northern Bobwhile exposed ( pceornffliudoernocoectiannteersvuallfoofna1t6e4wtaos2d8e9tperpmmi.neTdhteosbleop2e20ofptphemcwointchenatr9a5ti%onresponse curve was 7.005 and the chi-square value was 0.023. The no mmoorrttaalliittyy,csoingcnesntorfattoixoinciwtyaasn7d3.e2ffpecptms.upBoansebdoduypwoenigthrteagtamienntatretlhaete1d46 ppm. ppm test concentration, the no observed effect concentration was 73.2 Author andlor submitter: 3M Corporation, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY Reliability: Klimisch ranking = 1. 001371 REFERENCES Trheiqsuessttudofy twhaes3cMonCdoumcptaendya.t Wildlife International Ltd., Easton, MD at the OTHER Last changed: 5/3/00 001372