Document 7GZzwb62ZbNy8y6v63vKBvQ6

AR226-2979 u Font HLR 584-92 STATIC, ACVWWWmVNO iNil9,518 TO FATHEAD MIMNOW8. F^^hs^s^melas i2ata-Bfiflu!mm!3i Organisation for Economic Co-Operation and Development (OECD) Guideline for Testing Chemicals: 2|33 Author Keith B. Pierson, Ph.D. Studly Completed On tober30,1992 PerforminLgaboratory E. 1. du Pont as Nemours and Company Haskei! Laboratory for |roxicologyand industtiai Medicine Elkton Road, Post Office Box 50 NewarH, Delaware 19714 LaboratoryPropel ID Haskell Laboratory Report No. 584-92 Pige 1 of 18 ^ ^ QO5 &S' ^^eA- 00^ Du Pent HPt SBiNB GOOD TEMENT This study was condui Practice Standards (4 Laboratory Practice (C Laboratory Practice Sit noted below. Solubility and under storage conditiQrt^Ne substance in dilution water we,n. i made in accordance with Good I amendments and/or SOP devisi i noncompliance (if any) areal^ existed that affected the scientif ( A Good Laboratory Principles of Good Japan Good i the two exceptions in dilution water and itiofis of the test asurements were not lards. Protocol I. Areas of y records. No deviations Submitter: E. 1. du Pontde Nemouts ania-Company Sponsor: Du Po it Chemicals E. I. du Pontde Memouis and Company STUDY DIRECTOR: &^. ^^J Keith B. Pierson, Ph.D. Resesirch Toxicologist Environmental Sciences so ocr/w^ Mo/Day/Year ^Ss . ^awi ^ C^ ^ ,0^ Q^' 2 Du Font HLR 584-92 TA^LEOF CONTENTS Page Good Laboratory Practice Statemi Hit....................................................2 Table of Contents........................................................................3 General Information......................................................................4 Summary.................................................................................5 Quality Assurance Documentation ......................................................6 I. Purpose.........................................................................7 II. Records.........................................................................? HI. Materials Test and Methods .........................................................7 A. B. DilutiSonubstance..............................................................77 C. Animal CWultautreer............................................................... ..............................................................7 D. Test Methods............................. Chemical Analysis ..................................8 E. Statistical Analysis ..........................................................8 F. ..........................................................8 IV. Results and Discussion.........................................................9 V. Conclusion......................................................................9 VI. Literature Cited ........................................................... ....9 VH. Tables......................................................................... 10 1. Chemical Characteristics of Haskeli Laboratory Well Water............. 10 2. Measured Concentrations of H-19,518 in a 96 Hour, Static, WUnaateerraCtheedmTisetrsyto..f.t.h.e..D,.i.lu..ti.o.n.W..a.t^er..a.t..T.e..s.t.............................. 11 12 3. 4. Physical and Chemical Characteristics of Start...................... H-19,518 Test Solutions..... 13 5. Observed Mortality of Fathead Minnows, Pimephales promefas. Exposed to H-19,518 for 96 Hours in a Static, Unaerated Test.......... 15 6. Observed Effects of H-19,518 on Fathead Minnows, Pimephales prometas, for 96 Hours in a Static, Unaerated Test...................... 16 7. H-19,518 Concentrations Causing Mortality to 50% (LC50) of Fathead Minnows, Pirtiephales promelas, at Specific Time Intervals.... 17 ^ a VIII. ApPproetnocdoilxA.m..e..n.d..m..e.n.t.s..a..n.d...S.O..P.............................................. 18 18 1. Deviations.............................. ,,< ..is^0 A ^ 3 CO^^ s^ ||| @I al i || |i i > | 1|gg! If | 1 | | | 1Ls 2H fCoE s n ipu PontonSHLRR58W4P .92 o el Le "e A GENERAL INFOe RMATION Lar "i i [| ]i | | HaskalNumber: || H-19.518 | (om) Canpostrs| | | sane oo[--1 Conansmns | | ------ == ED-u1P.odnutPCohnetmdiecaNlesmours and Company Wilmington, Delaware 19898 `Study Initiated/Completed: | 87/-10-930-292 Inifisted/Completed:||8. -10/8--149-622 van, pos ecm TSA Du Font MM W^Z 1-19.519 melas H-19,518 was slightly to?ie|jia9^-||Mr, minnows. PimephaSQSpromsta?. Momii 18.5, 41.0,91 1, 203. ^StNtflW N were 1.51.6.96,15.8,.3ttJ|%2|^S|^ based on measured concentraS ons at 9 interval of 44.2 - 84.9 mg/L ; acute test using fathead ;entrations were 0,8.30, incentrations at 96 hours - The96-hourLC50 11.7 mg/L with a 95% fudicial Work By: ^f^^i^f^ ^^eteison, B. A, Associate A^ )Q/f Mo/Day/Year Work By: lO/cAt^ Mo/Oay/Year THI REPORT IS APPRROOVED FOR ISSUE. Wi ^.ftt^^ Keith BPiereon, Ph.D. Resear sh ToxiGologist and Study Director Enviror mental Sciences Jb Lr/?9^ Mo/Day/Year Company Sanilteed. Does not contain TSCACB1 5 Du Font HLR 584-92 QUAUTY ASSURANCE DOCUMENTATION ! (H-19518) r>?t??o* Inspection: Conduct - 8/10/92 Records. Report(s) -10/10/92 Finri^s reported to: Study Director -10/12/92 Management -10/30/92 ^ ^ ^ J U M ^ Reported ^ Rheav RobertC. duality Assurance Auditor ^1^^ Date' Company Sanitized. Does not contain TSCA CBI Pent mM SS4-93, The goal of this study minnows, PimephalQs All data and the final report wiil Toxicology and Industrial Du Pont Records 19,518 to fathead askeii Laboratory for archived at the ') A. Test Substance The test substance is described kinder General I n. The test substance was added to dilution water (see below) and stiFre^foiillD minutes to create a stock solution. Visual inspection suggested that the test substance was in solution in the stock. The stock solution was addelsto^iution water in appropriate volumes to make iniiividual test soiuSians. Stability and solubility of H-19,518 in dilution water and under storage conditioo^ were not determined. B. Dilution Water Water (Table 1) originated from the Hasketl Laboratory well. C. Animal Culture Fathead Minnows, Pimephales yromelas, were rearedin continuously flowinp well water at Haskell Laboratory. Adult fish resided in^9 L aquaria at approximately 25C and were fed frozen brine shnrnp^nemia sp.. Golden Gate Brand, Kordon Division, N||walek, Inc.; ttayw^Nll^lKAge)nerally twice daily, once on Saturday r'ld once on ed on poiyvinyl chloride tiles coated with sand. Tiies with d and incubated at approximately 25C in a vi/ater tath. Larvae wero approximately 21 C and were f||a iiewly hatehedi-IS Products, Glen Burnie, MD) ger eraliy twice ' " 39 L aquaria at (Aquarium 's and daily on weekends. 39 L glass Juveniles were aquaria (23-cm - vww(L) helq either Iinll . '-1-- i; S'_"O. -- _ x 25<W)^OTm 2&7?2&-.L!. c\jiii]r>cfUuiclaii^r fiimbceirygilcaigssa ticainnknso ouir i1n11 .- _ ;-v..i.;.;--.i"l~-7 (H- ))". at approximately 21 "C and were fed newly hatch id AilsflaaSE. ai^i^oMlezen brine shrimp generally twice daily on weekdays, once on SsstsS^/1^ once on Sunday. Sickness, injury, and abnormali f were not GtiserfeS. animals were identified by labels on aquaria and test vessels. 'notcoRtain.TSCACBl 7 Vv---V''^-^WvSif'vWmSia,, Pout HIJt584-^ D. Test Seven test concentratic were 0,8.30,1&5, 41.0^ 1.1 to 1.6 cm standard lei (mean 0.037 g). pr : Erienmeyer glass fla . . . , , , , -^ y.:' xisersrpf. ..^^SW^"' ,-,.. test solution {35-cm totally filled to minimize j prevent loss of potentif" added to each replicat< ,... total 10 fish perconcer Test solutions ranged ftfiBai^SS, i^l,^^^^ hours prior to nor durin^lhe^lst^llli^ darkness was employed w!lh25 ffiinLrtes. following the 16 hour light in^rwSi'^||fli^ and ,t)^i| daily. ^p' '''"^'' ," ^-i^'^ I Nominal concentrations Sontroi fish ranged from 0.051 g wet weight i] containing 4.4 Lot |rs. Each fiask was iSaranWrapto jnts. Ten fish were cafe per concentration, t g/L at test conclusion. H Fish were not fed 28.5 jrs light versus 8 hours |htpreceding and effects were noted Dissolved oxygen (YSI |^del54^^low1i|a^ Yellow Springs, OH) and pH (Coming Model Sl^'Oc^i^^ei^^iiliruments, Medfield, MA) were measured in the water<a3R(it^^|ilielfeaa^^ before fish were added at the beginning ofthetespri^a^lythere^^^mperature was meas'ired (American Socie^ofTJpstihg^alepalsresiNeN mercury thermometer) in the water cQQ&fQVm^ aH^lest^ince^l^ns before fish were added at the beginning oftihe^SR^cteH^i^^ end of the test. Total alkalinity (Hach kit, Hach^(^lernica(^l iia^ayN^lland, CO). EDTA NM^CO), hardness (Hach kit, Hach Chen^ <3on^any. and conductivity (Y81 Model 33, Yellow Springs Iriiai-umeafs. yellowip%A|8s,OH) of the dilution water were measured at the begii lining ottnelest; ^y^tinuously-recopding thermometer in a control vessel vi as used f cNeck^tNnperature variation during the 96-hour test. E. Chemical Analysis All analyses were conducted afte the test was conlillist^ at the Du Font Jackson Laboratory at Chamber's Wortics, DeepWisffiW^llftSKamples and standards were completely comf listed in ailydNigigj^i^gen Wickbold Torch. The resulting water was then anslyzedl^rioiatltedTiQet^oncentra^^ using an Orion fluoride ion selective electiode. Concentfations^ere then calculated from the standards using either a three or four point 1isa|||ratiocnurve. F. Statistical Analysis The 24-, 48-, 72-, and 96-hour LCSOs were calGuliSitedfy the SAS Probit method [2]. Company Sanitized. Does not contain TSCA C8I Font WM NW A rangafinding test with the top) was conducted' 100, and 1000 mg/L. Mi inflationsof 0.1.1,18, %, respectively. ^^^^fa^ Nominal concentrations i i.5. 41.0,91.1,203, 450, and 1000 mg/L. Me were 1.51,6.96, iected increase in 15.8,36.8,87.2,137,391 yfic cause for this measured cencenteationi^...,. increase was not determiris^, I,t,n,.i,a, stance was not complete!yin^otaonW^f^taB@| ance in the water control was noted, but chemical and physical parameters in th ere within expected ranges except for dissoiv solved oxygen concentration dropped as low as^ 2.3, and 4; however, these observation^ "r"' nee since fathead minnows can survive at much intrations for extended periods. Behavioral _ _ L50 was 61.7 mg/L with a^|^ 6. N_.g , ^ ^ ^ o r 3 | | S | B . 9 The mg/L 96-hour (Tables 5 and 7) based upon measured con>htrations at 96 holjfs. V. C3NCLUSEON H-19,518 was slightly toxic to iath< 96-hour, static, unaerated test. I minnows, Pri^||^s proms/as, in a VI. LITERATURE 01^ .-"i.,^;''.!:-- 1. OGrugidaenliisnaetifoonr TfoerstEincognCohmeimc iCcoal-sO:pe2@ra3ti. o4nAapnrdil19!e8i^<ipment(OECD). 2. Finney, D. J. 1971. ^robit Anals;sis, 3td Ed., GambTOdgeUniversity Press :; 333 p. Company Sanitized. Does not contain TSCA CB! 9 Du Font HLR 584-92 STATIC. AbUTE, 96-HOUR LC50 OF H-19,516 TO FATHEAD MINNOWS, PImephaSespromelas I TABLE 1 Chemical Characteristics of Haskelt Laboratory Well Water1 Parameter Concentration BOD. mg/L COD. mg/L DOC, mg/L TOC2, mg/L Total Kjeldahl N, mg/L Ammonia N, mg/L Turbidity, NTU Phenolics, mg/L Color, apparent Co/Pt7 Solids Total suspended, mg/L Aluminum, mg/L Antimony, mg/L Arsenic, mg/L Beryllium, mg/L Boron4, mg/L Cadmium4, mg/L Calcium, mg/L Chloride3. mg/L Chromium, mg/L Cobalt, mg/L Copper. mg/L Cyanide. mg/L Iron3, mg/L Fluoride3, mg/L <2 <10 1.4 2.9 0.8 <0.2 <1.0 <0.050 <5 <4 <0.100 <0.060 <0.01(& <0.001 0.020 <0.00^ 24.3 6.2 <0.010 <0.020 <0.010 <0.025 0.001 <0.1 Parameter Lead, mg/L Magnesium, mg/L MBAS/LAS. mg/L Mercury, mg/L Nickel, mg/L Nitrite3, mg/L Nitrate3, mg/L Phosphate3, mg/L Potassium, mg/L Selenium, mg/L Silver, mg/L Sodium, mg/L Sulfate3, mg/L Sulfide, mg/L Znc, mg/L Volatile priority pollutants Add extractable priority pollutants Base/neutral priority pollutants Pestiddes/PCBs Organophosphate pestiddes5, |ig/L Concentration <0.075 3.7 <0.05 <0.00020 <0.020 <0.1 1.9 <0.1 1.7 <0.005 <0.010 8.2 5.0 <0.10 <0.058 N0 ND6 ND6 ND6 <0.50 ^ate of sample collection 26 'May 1992 unless indicated otherwise, analyses performed at Environmental Testing and Certification Corporation, Edison, New Jersey; 2Qate of sample collection 1 July 1992, performed jat Du Font Engineering Test Center, Newark, Delaware; SDate of analyses 10 June 1992, analyses performed at Du Font Chemicals, Jackson Laboratory, Deepwater, New Jersey; ^elowthe Published Method Detection Limit (BMDL): SAnalyses performed at Hazleton Laboratories, Inc., Madison, Wl; fiNone detected; 7Units based on cobalt/platinum reference. 10 ^<WarSGACBt T^%^^,'^^^ i TABLED ', .uiQS'lBin8 ^^?!^eATest attimeoftdia^ortalfty asamptes^" j5oaNBBrSa^fizexL Does not contain TSCA CBl 11 Du Font HLR 584-92 STATIC, ACUTE. 96-HOUR LC&) OF H-19,518 TO FATHEAD M|NNOWS.Pimephales promelas I TABLE 3 Water Chemistry Of the Dilution Water at Test Start Total Alkalinity (mg/L as CaCOg) 80 EDTA Hardness : (mg/L as CaCOa) 78 Conductivity (umhos/cm) 170 )oes nQtcaiuaut laCACSl 12 Du Pont HLR 584-92 STATIC, ACUTE, 96-HOURLC50 OF H-1^,518 TO FATHEAD MINNOWS. Pin-sephaSespromefas |TABLE 4 Physical and Chentical Characteristics of H-19,518 test Solutions i Measured Concentration (mg/L) Time oHr 24 Hr 48 Hr 72 Hr 96 Hr Dissolved Oxygen, r-cA HgO Control 6.96 15.8 36.8 87.2 187 395 997 (1.51) 8.4 6.9 5.2 4.8 4.4 8.4 7.3 5.7 4.9 4.5 8.3 7.2 6.1 5.5 5.0 8.$ 7.4 6.6 5.8 5.1 8.5 7.4 6.4 5.9 5.1 a 8-4 7.5 6.7 6.5 a 7.7 a a a 8.4 a a 8.4 7.9 &H HgO Control 6.96 15.8 36.8 87.2 187 395 997 (1.51) 7.3 7.3 7.4 7.4 7.4 7.5 7.3 7.4 7.4 7.4 7.5 7.3 7.4 7.4 7.4 7.$ 7.3 7.4 7.4 7.4 7.$ 7.3 7.4 77..44 7a.4 7.$ 7.3 7a.4 a a 7.$ 7.3 a a a 7.6 7.3 fading was not made because of total mortality. ^mpa,ys,QtUti, "9tf-0^^ ^lain ^ 13 Da Font HLR 5P4-92 STATIC, ACUTE, & M-tOUR LC50 OP H-19,518 TO FATHEAD MINNOWS. Pimephales promelas TABLE 4, Continued Physical and Chemicil Characteristics of H-19,518 Tesit Solutions Measured Concentration (mg/L) Time 0 Hr \ 24 Hr 48 Hr 72 Hr 96 Hr Tftmpftrature.oc HgO Control 6.96 15.8 36.8 87.2 187 395 997 (1.51) 21.2 21.2 21.2 21.2 21.2 21.3 21.3 21.4 21.2 21.2 21.2 21.2 21.2 21.2 21.2 21.2 21.1 21.1 21.1 21.1 21.1 21.1 a a sReading was not made because of total mortality. 21.3 21.3 21.3 21.3 21.3 21.4 a a 21.3 21.3 21.3 21.3 21.3 21.3 a a Coffipao ^^eri.ooes 14 Du Font HLR 584-92 STATIC, ACUTE, TO FATHEAD Ml -HOUR LC50 OF H-19,518 3WS, Pimephales promelas TABLE 5 Observed Mortality of Fathead Minnows, Pimephales promelas, Exposed to H-19.518 for 96 Hours in a Static, Unaerated Test Measured Concentration (mg/L) Cumulative Mortality in Percent'1 ______:i______________ 24 Hours 48 Hours 72 Hours 96 Hours Water Control (1.51) 0 0 0 0 6.96 0 0 0 0 15.8 0 0 0 0 36.8 10 10 10 10 87.2 187 50 50 60 80 80 80 100 100 395 100 100 100 100 997 100 100 100 100 ^here were ten fiaii per test concentration at test start. Company sanded. Does not contain TSCACBj 15 "y'^m Du Font HLR 584-92 STATIC. ACUTE96J440URWSO OF 1-^9,518 TO FATHEAD MM|IMOWS^.----"^'-^n^ TABLE 9 Observed Effects foofr M96-1H9,o5u1r8s pnn aFaStthaetiacd, UMninaneroawtesd, PTIemset phales promeSas, kAaaei ireut Concentration (mg/L) I 24 Hours Number of Rsh With Effect 48 Hours 72 Hows Water Control (1.51) 6.96 15.8 36.8 87.2 187 395 997 0/10 0/10 0/1 C 0/9 0/5 2a/2 b b 0 0 0 0/9 23/5 2a/2 b b 0 0 0 3C/9 4M/4 b b b 96 Hours 0 0 0 2C/9 2a.d/2 b b b 'Partial toss of e'- jtlibrium. 'Total mortality. "Lying on bottom of the test vessels. ^rrattc swimming. compattysa-^.^ "^contain 16 1 ! u Font HLR 584-92 STATIC, ACUtTE, 96-HOUR LC50 OP H-19,518 TO FATHEAD MINNOWS. PirnQphssiffps wmelas | TABLE 7 l H-19.518 Concentrations Causing Mortality to 80% (LC50) of Fathead Minnows. PfmQphales promo/as, at Specific Time Intervals Time 24 Hours 48 Hours 72 Hours 96 Hours LC50 (mg/U*) 91.8 91.8 71.9 61.7 ^5%Fiducial Limits | (mg/L^ 61.9 134 61.9 134 50.9 101 44.2 84.9 Slope 3.38 3.38 4.93 5.82 Y-mtercept -6.63 -6.63 -9.15 -10.4 sBased upon measured concentrations at 96 hours. c<"l'p'--^ "to'c"^-^, 17 Du Font HLR 584-92 STATIC. AClljTE9,6-HOUR LC50 OF H.19,518 TO FATHEAD MINNOWS, PImephales pmmelas APPENDIX 1 PROTOCOL AMENDMENTS and SOP DEVIATIONS PROTOCOL AMENDMENT NUMBEORNE It is not necessary to weigh ti^hand determine test chamber loading prior to a. test start. Stability and solubility of the test substance in dilution water and under b. storage conditions will not be: determined. Samples will be taken from each replicate at test start and end for chemical c. analysis of the test substance concentration. aquatlcjfil '1. At test end surviving fish will be sacrificed according to L [ ^ e. Fish will be starved for 24 hours prior to test start. SOP DEVIATIONS Readings were not made to measure the amount of light present during the a. primary and transition lighting periods. Dissolved oxygen concentrations lower than 60% occurred in some test b. concentrations and were not reported to the Study Director. c. Water bath temperature was not recorded. d. Temperatures and equilibrium times for individual test chambers were not recorded. ^^^ 18 ln";^^.