Document 6kOzRVzGY1ka3rMk4LmyQYQd

BIO-TEST XaiatakAiai, Hue. REPORT TO MONSANTO COMPANY TWO-YEAR CHRONIC ORAL TOXICITY STUDY WITH ' AROCLOR 1254 IN BEAGLE DOGS NOVEMBER 1, 1971 IBT NO. C7299 I. Introduction A sample identified as Arocior 1254, Lot #AK-38, was received from the Monsanto Company for the purpose of conducting a two-year chronic oral toxicity study using purebred beagle dogs. The following report presents the results of the investigation.' GBRN002565 LEXOLDMON002845 BIO-TEST JniMntMiti, D*c. 2 21. Summary Two year* of feeding Aroclor 1254 at dietary level* of 1, 10 and 100 ppm to purebred beagle dog* revealed the following: A. Body Weight* There were no eonaiitent significant differences between the untreated control animals and animals in the three test groups with respect to body weights. B. Food Consumption The amounts of food consumed by the animals in the three test groups did not differ significantly from that consumed by the controls. C. Behavioral Reactions . No untoward behavioral reactions were noted at any time during the investigation. D. Mortality Two fatalities occurred during the study, a 100 ppm female after 33 weeks of testing and a 10 ppm female after 37 weeks. The fe male receiving 100 ppm died from injuries received in a fight. Gross and histologic examinations of the 10 ppm female revealed moderate chronic peritonitis. . Hematologic Studies No significant abnormalities were noted at any of the levels tested. SBRN0D2566 LEXOLDMON002846 BIO-TEST AAnn/miW, Sme. 3 F. Blood Chemistry Studies Animals receiving Arodor 1254 at all three dietary levels exhibited blood chemistry values comparable to those of the untreated control animals. C. Urine Analyses There were no significant differences between the urine of the test and untreated control dogs. H. Pathologic Studies 1. Organ Weight Data The organ weight data for the animals in the untreated control and three test groups did not differ significantly. 2.. Gross and Histopathologic Findings . No consistent or dose related abnormalities attributable to the ingestion of Aroclor 1254 were noted among any of the animals. Respectfully submitted, INDUSTRIAL BIO-TEST LABORATORIES, INC. Report prepared by: Bruce R. Burtner, Ph.B. Assistant Toxicologist Dog Toxicity Report approved by:' e Dennis C. Lindberg, B.S. _ / Senior Group Leader Dog Toxic ity November 1, 1971 M. L. Keplirfger, Pn7D. Manager, Toxicology 6BRN002567 LEXOLDMON002847 4 HI. Procedure A. Organization The two year toxicity study utilized an untreated control group and three test groups, each consisting of eight purebred beagle dogs (four males and four females). The beagles were all eligible for A.K. C. registration and had been previously immunized against rabies, distemper, infectious canine hepatitis and leptospirosis. All dogs were acquired from our own colony and were under observation for two weeks prior to the start of the investigation, during which time they were re-immunized and rendered clinically free of any existing parasitic infestation. . During the investigation, the selected animals were housed in kennels equipped with outside runs, four dogs of the same sex and group being accommodated in a single kennel. . The material to be tested, Aroclor 1254, was incorporated into a stock diet and fed to the dogs seven days a week at three graded dietary levels. The levels were 1, 10 and 100 ppm. An outline of the test organization and diet composition is presented in Table I. GBRN0D256B LEXOLDMON002848 Group uc I 11 III TABLE I TEST MATERIAL: Aroclor 1254 Two-Year Chronic Oral Toxicity Study - Beagle Doga Teat Organization and Diet Compoaition Number of Animals Tested Males Females ,4 4 Dietary Constituents, percent (w/w) Aroclor 1254 Stock Food* None 100.0000 44 0.0001 99.9999 44 0.0010 99.9990 44 0.0100 99.9900 * Purina Dog Meal, Ralston Purina Company, St. Louis, Missouri. LEXOLDMON002849 G1 to XI Zo o rino u< o <0 fa&ttfcal BIO-TEST 2* 6 B. Parameters Investigated Initially, the body weight of each dog in every group was determined and recorded. Thereafter, weighings were conducted weekly for the duration of the test. * At the beginning of each week, the appropriate dietary constituents for each of the groups were thoroughly blended in a Hobart mixer. .Pre weighed amounts were distributed into self-feeding units and maintained in excess of the animals' consumption. One such unit was available to the dogs in each kennel for three hours each day. During these threehour intervals, food was consumed on an ad libitum basis. At the end of each seven-day period, all unconsumed food was collected and weighed. Food consumption was then calculated and recorded. Water was available to the animals at all times. The dogs were under observation during the investigation and were examined daily for clinical signs or symptoms indicative of systemic toxicity. The following determinations were conducted upon each dog from the untreated control group and three test groups just prior to the inception of the study and after 1, 3, 6, 12, 18 and 24 months of testing: ` Hematologic Studies total leukocyte count erythrocyte count ` hemoglobin . hematocrit differential leukocyte count GBRNQ02570 LEXOLDMON002850 BIO T E S T 5*e. 7 Blood Chemistry Studies blood urea nitrogen serum glucose serum alkaline phospha.ta.se serum glutamic - oxalacetic transaminase serum glutamic - pyruvic transaminase Urine Analyses albumin glucose pH specific gravity microscopic elements - leukocytes erythrocytes crystals At the conclusion of the investigation, the dogs from each group were sacrificed by electric shock. All major tissues and organs were examined grossly. The weights of the following organs were obtained: liver, kidneys, heart, brain, spleen, gonads, adrenal glands, thyroid gland and pituitary gland. The following tissues and organs excised from these animals were examined histologically (Hematoxylin*Eosin stain): Adrenal glands Aorta (thoracic) Bone marrow (sternum) Brain (cerebrum, cerebellum, pons) Caecum Colon ' Esophagus .' GaU bladder * Gonads ` Heart Kidneys Liver Lymph nodes (cervical, mesenteric) Muscle ' Pancreas Peripheral nerve (sciatic) Pituitary gland Prostate gland Salivary gland (submasdllary) Small intestine (duodenum, jejunum, ileum) Spinal cord Spleen Stomach (cardia, fundus, pylorus) Trachea Thyroid gland Uterus Urinary bladder In addition, samples of liver, kidneys, muscle and fat were frozen and saved for chemical residue analyses. 6BRNQ02571 LEXOLDMON002851 jyUW BIO-TEST A^iaAtiW, Shc. 8 IV. Re salts A. Body Weight Data Body weight data are presented in Tables II and III. It is noted that the mean overall weight gain for test group in males (100 ppm Aroelor 1254) is lower than that of untreated control males. However, the individual gains for untreated control males show a wide divergence, particularly for that of dog 4. Upon elimination of this animal's gain, it is felt that the overall mean weight gain for test group m males is comparable to that of untreated control males. A similar situation is noted between untreated control and test groups III females. Upon eliminating divergent individual gains (animals 8 and 29) overall mean weight gains for the two groups are comparable. It is felt, therefore, that weight gains in test group in are essentially comparable to those of untreated controls and that differences here or in lower levels are not the result of test material ingestion. 6BRN002572 LEXOLDMON002852 TABLE II s 3 1 o i si w i f TEST MATERIAL; Aroclor 1254 Two-Year Chronic Oral Toxicity Study - Beagle Dogs Body Weight Data for Males, kilograms Dietary Level Group (ppm) UC None I1 II 10 III 100 Age at Inception Dog of Test Body Weights at Week Indicated: Number (months) 0 1 2 3 4 5 6 7 8 9 10 11 12 1 2 3 4 Mean 6. 5 6.4 6. 5 6.8 6.8 6.8 7.0 6.9 7.0 6.8 7.0 7.2 7.3 7.4 6.0 7. 1 7.0 7.4 7.2 7.7 8.0 7.8 8.0 8. 1 8.4 8.7 8.8 8.9 4.8 6.3 6.6 6.9 7. 1 7.3 7.5 7.3 7.8 8.3 8.2 8.3 8.8 8.8 4.8 7.8 7.9 8.4 8. 5 8.9 9.2 9.4 10.0 9.8 10.4 10. 5 11.0 11.0 5. 5 6. 9 7.0 7.4 7.4 7.7 7.9 7.9 8.2 8.2 8.5 8.7 9.0 9.0 9 10 11 12 Mean 9.0 10.0 10.2 10. 5 11.0 11.2 11.3 11.4 11.3 11.7 12.0 12.4 12.4 12.2 6.8 10.0 10.0 10.2 10.4 10.6 10.5 10.3 10.2 9.6 10. 5 10.7 11. 1 11.0 6. 5 9.3 9.8 10.0 10.3 10.2 10.2 10.4 10. 5 11.0 11.2 11.5 11.8 11.6 6.0 9.4 9.8 10.2 10.6 11.0 11.2 11.4 11.5 11.7 11.8 12.4 12.2 12.4 7. 1 9.7 10.0 10.2 10.6 10.8 10.8 10.9 10.9 11.0 11.4 11.8 11.9 11.8 17 18 19 20 Mean 6. 5 7.7 8.0 8.4 8.7 9.0 9.2 9.3 9.5 9.8 9.9 10.2 10.9 10.7 5.8 7.7 8.2 8.6 8.7 8.6 9.2 9.4 9.4 9.6 9.9 9.8 10.5 10.4 4.8 6.9 7. 1 7.4 7.8 8. 1 8.4 8.7 8.8 9. 1 9.2 9.6 9.9 10.1 6.0 8.3 8. 1 8.2 8.4 8.7 8.9 8.8 8.8 9.5 9.6 9.6 9.9 9.7 5.8 7.6 7.8 8.2 8.4 8.6 8.9 9.0 9. 1 9. 5 9.6 9.8 10.3 10.2 25 26 27 28 Mean 6.8 6.7 6.8 7. 1 7. 3 7.4 7.3 7.3 7.4 7.6 7.4 7. 5 7.2 7.6 6.8 6. 3 6.2 6.6 `6.8 6. S 7.0 7.0 7.0 7.2 7.2 7.4 7.5 7.4 6.0 7.0 7. 1 7.2 7.3 7.3 7.2 7.1 7.0 6.8 6.4 5.8 6.0 6.0 5.8 6.8 7. 2 7.6 7.8 7, 8 8. 1 7.8 8.2 8.3 8.0 8. 3 8.3 8.6 6. 4 6. 7 6.8 7. 1 7.3 7.3 7.4 7.3 7.4 7. 5 7.2 7.2 7.2 7.4 LEXOLDMON002853 TABLE II continued TEST MATERIAL: Aroclor 1254 Two-Year Chronic Oral Toxicity Study - Beagle Dogs Body Weight Data for Males, kilograms si'C 'r B iy B H fY 1 S3 1 " 100 Group uc Dietary Level (ppm) Dog Number None 1 2 3 4 Mean Body Weights at Week Indicated: 13 14 1? 16 17____18____19___ 20___ 21____22__23_ 24 25 -26- 7. 1 7.5 7.3 7.3 7.4 7.4 7.2 6.9 7. 1 7.0 7.2 7. 1 7.2 7.0 8.8 9.1 9.2 9.4 9.5 9.4 9.6 9.5 9.7 9.7 9.9 9.8 9.6 10.0 8.8 9.0 9.2 9. 1 9.6 9.4 9.7 9.6 9.7 9.6 10. 1 9.6 9.9 9.9 11. 1 11.5 11.5 12.0 12.0 12.4 12.9 12.8 12. 5 12. 1 12.8 13.0 13. 1 13.0 9.0 9.3 9. 3 9.4 9.6 9.6 9.8 9.7 9.8 9.6 10.0 9.9 10.6 10.0 I 1 9 12.2 12.4 12.0 12. 3 12.6 12.4 12.7 12.4 12.6 12.5 12.8 13.3 12.7 12.7 10 10.4 11.0 10.8 11.0 11.4 11.6 11.8 11.7 11.9 11.7 12.0 12.3 12.0 12.0 11 11.5 12.0 12.0 12.0 12.0 12.3 12.4 12.4 12.3 12.3 12.6 12.8 12. 5 12.5 12 12.2 12.5 12. 5 13.0 13.2 13.3 13. 5 13.7 14.0 13.9 14.0 14.0 14.3 14. 1 Mean 11.6 12.0 11.8 12. 1 12.3 12.4 12.7 12.6 12.7 12.6 12.8 13. 1 12.9 12.8 II 10 17 10.6 10.8 10.7 11.1 11. 1 11.5 11.4 11.6 11.5 11.5 11.6 11.6 11.7 11.5 18 10.3 10.8 10. 5 10.5 10.4 10. 3 9.4 8.0 7.2 9.6 11.2 11.2 11.8 12.0 19 10.0 10.3 10.4 10.6 10. 5 10.8 10.9 10.9 10.7 10.6 11.0 10.9 11. 1 11. 1 20 9.6 10. 0 10.0 10. 1 9.9 10. 1 10. 1 10.2 10.3 10. 1 10.3 10.3 10.4 10.3 Mean 10. 1 10.5 10.4 10.6 10.6 10.7 10.4 10.2 9.9 10.4 11.0 11.0 11.2 11.2 III cCnO z o No) vj G 100 25 7.5 7.7 7.6 7.6 7.3 7.5 7.5 7.5 7.8 7.6 7.8 7.7 7.7 7.7 26 7.3 7.6 7.4 7.4 7.3 *7. 5 7.6 7.6 7.7 7.6 7.7 7.6 7.6 7. 5 27 6.0 6.3 6. 3 6.3 6. 3 6.6 6.8 6.9 7.1 7. 1 7.2 7.3 7.4 7. 3 28 8.4 8.6 8.4 8.6 8. 3 8.4 8.9 9. 1 9.2 9.2 9.4 9.4 9.4 9.3 Mean 7.3 7.6 7.4 7. 5 7. 3 7. 5 7.7 7.8 8.0 7.9 8.0 8.0 8.0 8.0 LEXOLDMON002854 TABLE II continued TEST MATERIAL: Aroclor 1254 Two-Year Chronic Oral Toxicity Study - Beagle Dogs Body Weight Data for Males, kilograms 1 S 3 1 * fi 13 '9( Group UC I II III Dietary Level (ppm> Dog Number Body Weights at Week Indicated; 27 28 29 30 31 32 33 34 35 36 37 38 3? 40 None 1 2 3 4 Mean 7.4 6.9 7.2 7.0 7. 1 7.2 7.2 7.2 7.1 7.5 7.4 7.4 7.3 7. 1 9.8 9.8 10.0 10.0 10.0 9.9 9.8 9.7 10.0 9.9 10. 1 9.7 10.0 9.9 9.9 9.8 10.0 10.0 10.0 9.9 9.7 9.7 10.0 10.2 10.2 10.2 10.3 10.2 13.9 13.0 13.4 13.2 13.0 12.9 12.6 13.2 13.5 13.8 13.6 13. 5 13.7 13.4 10.2 9.9 10.2 10.0 10.0 10.0 9.8 10. 1 10.2 10.4 10.3 10.2 10.3 10.2 1 9 12.8 12.9 12.9 12.8 12.7 12.0 12. 1 12.5 12.8 12.6 12.3 12.2 12.0 12.3 10 12.0 12.0 12.0 11.9 12.0 12.0 11.9 12.0 11.9 11.9 11.6 11.6 11.8 11.6 11 12.6 12.7 12.6 12.8 12.7 12.6 12.2 12.2 12.5 12.3 12. 5 12. 5 12.8 13. 1 12 14.3 14.6 14.2 14.6 14.5 14. 1 14. 1 14.6 14.6 14.4 14.5 14.4 14.7 14.6 Mean 12.9 13.0 12.9 13.0 13.0 12.7 12.6 12.8 13.0 12.8 12.7 12.7 12.8 12.9 10 17 11.6 11.3 11.5 11.3 11.3 11.2 11.3 11.5 11.6 11.7 11.5 11.6 11.5 11.5 18 12.3 12.7 12.7 12.8 12.7 12.6 13.0 13.5 13.9 13.7 13.5 13.9 13.9 13.8 19 11.2 11. 1 11.0 11.0 10.9 10.7 10.8 10.9 11.2 11.4 11.3 11.0 11.2 10.9 20 10.4 10.3 10.7 10. 5 10. 5 10.4 10.6 10.8 10.7 10.7 10.4 10. 5 10. 5 10. 5 Mean 11.4 11.4 11.5 11.4 11.4 11.2 11.4 11.7 11.8 11.9 11.7 11.8 11.8 11.7 100 25 7.8 7.9 7.6 7.7 7.7 7.8 7.9 7.5 7.8 8.0 8.0 8.2 8. 1 8. 1 26 7.5 7.6 7. 5 7. 5 7. 5 ' 7.5 7.5 7.6 7.7 7.8 7.8 7.8 8.0 7. 5 27 7.7 7.8 7. 5 7.6 7. 5 7.5 7.6 7.6 7.7 7.9 7.8 7.8 7.8 7.8 28 9. 1 9.3 9.3 9.4 9.4 9.4 9.5 9.5 9.6 10.0 9.8 9.8 9.8 9.8 Mean 8.0 8.2 8.0 8.0 8. 0 8.0 8. 1 8.0 8.2 8.4 8. 4 8.4 8.4 8.3 LEXOLDMON002855 IS 31*018 r W 'T * TABLE II continued TEST MATERIAL; Aroclor 1254 ' Two-Year Chronic Oral Toxicity Study > Beagle Doga Body Weight Data for Males, kilograms Group uc Dietary Level (ppm) Dog Number None I 2 3 4 Mean Body Weights at Week Indicated: 41 42 43 44 45 46 47 48 49 50. 51 52 53 54 7. 4 7.5 7. 6 7. 5 7.4 7. 4 7.5 7.5 7.8 7.5 7. 5 7.9 7.8 7.6 10. 0 10.0 10. 0 9. 9 10.0 10. 0 10. 1 10.0 10.4 10.1 9.7 9.9 10.4 10.2 10. 2 10.4 10. 2 10. 4 10.2 10. 1 10.4 10.3 10.5 10.1 10.3 10.7 10.6 10.4 13. 5 13.6 13. 6 13. 5 13.4 13. 2 13.4 13.3 13.7 13.6 13.8 14.1 14.4 14.4 10. 3 10.4 10. 4 10. 3 10.2 10. 2 10.4 10.3 10.6 10.3 10.3 10.6 10.8 10.6 I 1 9 12. 1 12.0 12. 6 12. 6 12.3 12. 4 12.7 12.7 12.7 12.6 12.4 12.6 13.1 13. 1 10 11. 7 12.0 12. 3 12. 2 12.0 12. 0 12. 1 12. 1 12.5 12.3 12.5 12.5 12.6 12.3 11 12. 5 12.8 13. 0 13. 1 13.0 12. 9 13. 1 13.2 13.4 13.3 13.0 13.2 13.3 13. 1 12 14. 0 14.3 14. 6 14. 7 14.9 15. 1 15.2 15. 5 15.6 15.3 15. 5 15.8 16.0 15.6 Mean 12. 6 12.8 13. 1 13. 2 13.0 13. 1 13.3 13.4 13.6 13.4 13.4 13.5 13.8 13.5 II 10 17 11. 6 11.8 12. 0 11. 9 11.7 11. 6 11.9 11.8 12.2 12. 1 11.9 12.3 12.1 12.1 18 13. 8 14.3 14. 3 14. 3 13.8 13. 8 14.3 14. 1 14.5 14.2 14.2 14.4 14.4 14.3 19 11. 0 11.2 11. 5 11. 6 11. 1 11. 2 11.7 11.7 11.7 11.6 11.5 12.0 12.0 12.2 20 10. 5 10.3 10. 4 10. 6 10.6 10. 5 10.7 10.6 10.7 11.0 10.6 10.9 10.7 10.7 Mean 11. 7 11.9 12. 0 12. 1 11.8 11. 8 12.2 12.0 12.2 12.2 12.0 12.4 12.3 12.3 111 100 25 8. 0 8.0 8. 0 8. 1 7.9 7. 8 *7.8 7.3 7.5 7.3 7.3 7.6 7.3 7.2 26 7. 9 7.9 7. 9 8. 0 7.8 *7. 8 7.9 7.7 7.9 7.6 7.8 7.6 7.8 7.8 27 7. 8 8.0 8. 0 8. 0 7.9 7. 9 8.0 8.0 8.0 8.0 8.0 8. 1 8.2 8. 1 28 9. 2 8.7 9. 0 9. 4 9.5 9. 2 9.6 9.5 9.4 9.3 9.5 9.9 9.9 9.9 Mean 8. 2 8.2 8. 2 8. 4 8.3 8. 2 8.3 8. 1 8.2 8.0 8.2 8.3 8.3 8.2 LEXOLDMON002856 hdiohial BIO -TEST LEXOLDMON002857 TABLE II continued TEST MATERIAL: Aroclor 1254 Two-Year Chronic Oral Toxicity Study - Beagle Doga Body Weight Data for Males, kilograms Group UC I n m Dietary Level (ppm) None 1 10 100 Dog Body Weights at Week Indicated: Number 55 56 57 58 59 60 61 62 63 64 65 66 67 68 1 2 3 4 Mean 7.5 7..7 7.7 8.0 7.8 7.8 7.9 7. 8 7.9 7.9 7.6 7.8 8.1 8.0 10.0 10..2 10.1 10.1 10.2 10.1 10.2 10. 1 10.2 10.3 10.0 10.0 10.3 10.1 10.2 10. 3 10.2 10.4 10.4 10.5 10.3 10. 1 10.1 10.1 10.4 10.4 10.4 10.5 14.0 14. 0 14.1 14.0 14.2 14.1 14.4 14. 1 14.0 14.1 14.2 14.2 14.3 14.2 10.4 10. 6 10.5 10.6 10.6 10.6 10.7 10. 5 10.6 10.6 10.6 10.6 10.8 10.7 9 10 11 12 Mean 12.. 5 12. 8 12.2 12.5 12.6 12.6 12.4 12. 9 13.2 12.6 12.7 12.7 12.8 12.6 12.5 12. 8 12.3 12.5 12.4 12.3 12.2 12. 2 12.3 12.2 12.2 12.6 12.6 12.3 13.2 13.,3 13.1 13.0 12.8 13.0 13.2 13. 1 13.3 13.0 12.9 13.3 13.3 13.1 15.5 15. 4 15.4 15.4 14.7 15.2 15.3 15. 5 15.4 14.5 14.7 15.0 15.2 14.9 13.4 13. 6 13.2 13.4 13.1 13.3 13.3 13. 4 13.6 13.1 13.1 13.4 13.5 13.2 17 18 19 20 Mean 12.2 12. 2 11.9 12.1 12.0 11.9 12.1 12. 0 12.1 11.9 11.6 11.9 12.0 11.8 14.7 14. 3 14.3 14.4 14.5. 14.3 14.3 14. 2 14.4 14.4 14.2 14.5 14.4 14.3 11.9 12. 2 12.0 12.1 12.2 12.2 12.2 12. 1 12.0 12.0 11.9 12.2 12.3 12.2 10.3 10. 6 10.6 10.6 10.7 10.7 10.7 10. 5 10.6 10.7 10.5 10.7 10.8 10.6 12.3 12. 3 12.2 12.3 12.4 12.3 12.3 12. 2 12.3 12.2 12.0 12.3 12.4 12.2 25 26 27 28 Mean 7.5 7. 7 7.8 8.1 8.0 8.0- 7.9 8. 1 7.9 8.0 7.9 8.0 8.1 8.2 7.7 7.,7 7.6 7.7 7.8' 7.9 7.8 8. 0 7.9 7.9 7.7 7.8 7.8 7.8 7.8 7. 8 7.9 7.8 7.8 7.8 7.9 7. 9 7.7 7.8 7.6 7.9 7.8 7.7 9.7 9. 5 9.6 9.5 9.6 9.7 9.8 9. 6 9.5 9.4 9.4 9.8 9.3 9.2 8.2 8. 2 8.2 8.3 8.3 8.4 8.4 8. 4 8.2 8.3 8.2 8.4 8.2 8.2 ' r Shin it ia l B IO -T E S T LEXOLDMON002858 TABLE II continued TEST MATERIAL: Aroclor 1254 Two-Year Chronic Oral Toxicity Study - Beagle Doga Body Weight Data for Males, kilograms Group UC I II III Dietary . Level Dog Body Weights at Week Indicated: (ppm) Number 69 70 71 72 73 74 75 76 77 78 79 80 81 82 None 1 2 3 4 Mean 7.8 9.9 10.5 14.2 10.6 8. 1 9.9 10.5 14.4 10.7 8. 1 10. 3 10. 5 14. 5 10. 8 7.6 9.7 10.2 14. 1 10.4 7.6 10.0 10.5 14.4 10.6 7.9 10. 1 10.4 14.5 10.7 7.8 10. 1 10.4 14.3 10.6 7.8 10. 1 10.5 14.4 10.7 7.8 10.0 10.4 14.4 10.6 7.4 9.7 10.2 14. 1 10.4 7.5 9.7 10.2 14. 1 10.4 7.4 9.2 9.9 13.2 9.9 7.6 9.2 9.9 13.4 10.0 7.6 9.3 10. 2 13. 5 10. 2 1 9 13.0 13.0 12. 7 12.6 12.8 12.8 12.6 12.8 12.5 12.7 12.9 12.5 13.0 13.0 . 10 12.5 12.6 12. 5 12.2 12.4 12.2 12.0 12.0 11.8 11.7 11.9 11.6 12.0 11.9 11 13. 1 13. 1 12. 9 12.9 13.0 13.0 13.0 13.0 13.0 13. 1 13. 1 12.9 12.6 12.6 12 15.5 15.6 15. 4 15.7 16.0 16.2 15.7 15.9 15.9 15.6 16.0 16.2 16.2 16.0 Mean 13.5 13.6 13. 4 13.4 13.6 13.7 13.3 13.4 13.3 13.3 13.5 13.3 13.4 13.4 10 17 12.0 11.9 11. 8 11.8 12.0 12. 1 12.0 12. 1 12.0 11.9 12. 1 12.0 12.0 12.2 18 14.5 15.0 14. 8 14.5 14.5 14.5 14.6 14.6 14.5 14.5 14.5 14.6 14.5 14. 2 19 12.0 12. 1 12. 2 12. 1 12. 3 12.4 12.0 12.3 12. 1 12.0 11.7 11.5 11.9 11.5 20 10.5 10.7 10. 7 10.8 10.7 10.4 10.8 10.8 10.8 10.9 11. 1 10.5 10.5 10.8 Mean 12.2 12.4 12. 4 12.3 12.4 12.4 12.4 12.4 12.4 12. 3 12.4 12. 2 12.2 12.2 100 25 8.0 8.4 8. 3 8.4 8.2 ,8.3 ' 8.6 8.5 8.3 8.0 8.4 7.9 7.9 7.8 26 7.9 8.0 7. 9 7.9 7.8 7.9 8.0 7.9 7.9 7.9 7.9 7.5 7.7 7.6 27 7.7 . 8. 1 8. 0 7.9 7.9 8.2 8. 1 8.0 8.0 8. 1 8. 1 7.8 7.9 8.2 28 9.3 9.6 9. 7 9.8 9.2 9.4 9.4 8.9 9.0 9. 1 9.5 9. 2 9.5 9.4 Mean 8.2 8. 5 8. 5 8.5 8.3 8.4 8.5 8.3 8.3 8.3 8.5 8. I 8.2 8. 2 B IO -T E S T LEXOLDMON002859 TABLE II continued TEST MATERIAL: Aroclor 1254 Two-Year Chronic Oral Toxicity Study - Beagle Dogs Body Weight Data for Males, kilograms Group UC Dietary Level *PPm> None I 1 II 10 Iii 100 Dog Number 1 2 3 4 Mean 9 10 11 12 Mean 17 18 19 20 Mean 25 26 27 28 Mean Body Weights at Week Indicated: 83 84 85 86 87 88 89 90 91 92 93 94 95 7. 4 7. 6 7. 6 7. 6 7. 6 7. 6 7. 6 7.6 8. 0 7.5 7.6 7. 8 7.7 9. 5 9. 4 9. 6 9. 9 9. 9 10. 0 10. 0 9.9 10. 4 10.0 10.3 9. 9 10.2 10. 2 10. 2 10. 1 10. 5 10. 5 10. 4 10. 2 10.5 10. 6 10.5 10.5 10. 5 10.4 13. 9 13. 6 13. 9 14. 0 14. 1 14. 2 14. 3 14.5 15. 0 14.4 14.8 14. 8 15.2 10. 2 10. 2 10. 3 10. 5 10. 5 10. 6 10. 5 10.6 11. 0 10.6 10.8 10. 8 10.9 13. 0 12. 6 12. 8 12. 5 12. 4 12. 0 11. 6 12.0 12. 0 12.0 12.3 12. 5 12.9 12. o 12. 0 12. 0 12. 4 12. 2 12. 2 12. 3 12.5 12. 9 12.5 12.5 12. 5 12.7 13. 0 13. 2 13. 0 13. 5 13. 1 13. 1 13. 5 13. 1 13. 3 13.2 13.0 13. 2 13.2 16. 3 16. 3 16. 2 16. 0 16. 0 15. 9 15. 9 16.4 17. 3 16.9 17.0 17. 1 17.0 13. 6 13. 5 13. 5 13. 6 13. 4 13. 3 13. 3 13.5 13. 9 13.6 13.7 13. 8 14.0 12. 2 12. 2 12. 1 12. 2 12. 2 12. 2 12. 3 12.4 12. 7 12.4 12.5 12. 5 12.7 13. 6 14. 5 14. 5 14. 7 14. 7 14. 7 14. 6 14.8 14. 9 14.2 14.4 14. 5 14.7 11. 7 11. 6 11. 5 11. 5 11. 7 11. 9 12. 0 12.1 12. 5 12.2 12.2 12. 2 12.0 10. 7 10. 9 10. 8 10. 6 10. 6 10. 5 10. 4 10.9 11. 0 11.0 10.9 10. 7 10.9 12. 0 12. 3 12. 2 12. 2 12. 3 12. 3 12. 3 12.6 12. 8 12.4 12.5 12. 5 12.6 fi.8. 3 8. 5 8. 5 8. 6 5 8. 5 8. 4 8.3 8. 5 8.3 8.5 8. 4 8.7 .7. 5 7. 8 7. 6 7. 8 7. 8 7. 8 7. 8 8.0 8. 0 7.9 7.8 7. 6 8.0 6 2 7. 9 8. 0 8. 0 8. 0 8. 0 7. 7 8.0 7. 9 7.6 7.8 8. 0 8.0 9. 2 9. 5 9. 6 9. 4 9. 2 9. 2 9. 3 9.7 9. 5 9.5 9.5 9. 5 9.6 8. 3 8. 4 8. 4 8. 4 8. 4 8. 4 8. 3 8.5 8. 5 8.3 8.4 8. 4 8.6 in B IO -T E S T JU LEXOLDMON002860 TABLE II continued t TEST MATERIAL: Aroclor 1254 Two-Year Chronic Oral Toxicity Study - Beagle Doga Body Weight Data for Males, kilograms Group Dietary . Level Dog (PPm) _ Number- UC None 1 2 3 4 Mean I 19 10 11 12 Mean II 10 17 18 19 20 Mean III 0) GO oo7z) _ IN)--------- in at 100 25 26 27 28 Mean Body Weights at Week Indicated: 96 97 98 99 100 101 102 103 104 7.8 7.8 7.6 7.6 7.6 7.5 7.6 7.7 7.6 .10.0 .10.0 9.8 10.0 9.8 9.8 9.9 10.9 10.0 10. 2 10.2 10. 1 10.0 10. 1 10. 1 10.2 10.3 10.4 15.0 14.7 14.5 14.2 14.4 14.4 14.4 14.6 14.8 10.8 10. 7 10.5 10.4 10. 5 10.4 10. 5 10.6 10.6 12.8 13.2 13.0 13. 3 13.0 13.2 13.1 13.0 13.2 12.4 12.5 12.0 12.5 12. 1 12.2 12.5 12. 1 12.4 13.1 13.1 13.0 13.0 13. 1 12.9 13.1 13.0 13.3 17. 1 17. 3 17.0 17. 1 16.9 16.8 16.9 17.0 17. 1 13.8 14.0 13.8 14.0 13.8 13.8 13.9 13.8 14.0 12. 5 12.5 12.2 12.3 12. 1 12.2 12.3 12.5 12.4 14.0 14.5 14.3 14.2 14. 1 14.4 14.6 14.5 14.5 12. 2 12.2 11.5 11.6 11.6 11.7 11.9 12.0 11.7 10.7 10.7 10.2 10.9 10.9 10.7 10.4 10.7 10.9 12.4 12.5 12.0 12. 2 12.2 12. 2 12.3 12.4 12.4 8.6 8.7 8.5 8.6 8.6 8. 2 8.3 8.5 8.7 7.8 8.0 7.5 8.2 8.0 8. 1 8.1 8.0 8.0 8.0 8.0 8.3 7.6 7.6 7.6 7.7 8.0 8. 2 9.6 9.2 9.5 9.6 9.5 9. 7 9.6 9.7 9. 8 00 Ul 8. 5 8. 5 8.4 8.4 8.4 8.4 8.6 8.7 Overall Weight Gain 1.2 2.9 4,1 7.0 3.7 3.2 2.4 4.0 7.7 4. 3 4.7 6.8 4.8 2.6 4.8 2.0 1.7 1. 2 3.0 2.0 o LEXOLDMON002861 I Group uc I 11 III GM) X zo o IUS)l 00 Dietary Level Dog (ppm) Number None 5 6 7 8 Mean 1 13 14 15 16 Mean 10 21 22 23 24 Mean 100 29 30 31 32 Mean TABLE III TEST MATERIAL: Aroclor 1254 Two-Year Chronic Oral Toxicity Study - Beagle Doga Body Weight Data for Females, kilograms Age at Inception of Test Body Weights at Week Indicated: (months) 0 1 2 3 4 5 6 7 8 Q in 11 12 6.0 6.4 6.6 6.8 6.8 7. 1 7.3 7.2 7.5 7.4 7.5 7.5 7.3 6.7 6.0 7. 1 7.5 7.8 7.9 8.2 8.4 8.8 9.0 8.9 8.9 8.9 9.0 9.0 4.8 6.5 6.4 6.9 7.2 7.3 7.4 7.4 7.8 7.7 7.7 7.7 7.9 8. 1 4.8 7.2 7.4 7.7 7.8 8.2 8.3 8.2 8.8 8.8 9.2 9.3 9.7 9.8 5.4 6.8 7.0 7.3 7.4 7.7 7.8 7.9 8.2 8.2 8.3 8.4 8.5 8.4 6.8 8.3 8.3 8.4 8.3 8.5 8.6 8.7 8.5 8.6 8.9 9.2 9. 1 9.2 6.8 8.7 9.3 9.2 9.5 9.5 9.5 9.4 9.8 10.0 9.6 9.8 9.9 10.1 6.0 9.3 9.6 9.6 9.7 9.7 10. 1 9.9 10.3 10.4 10.5 10. 5 10.7 10.9 6.0 8.7 8.8 8.8 9.2 9.5 9.6 9.3 9.8 9.7 10.0 9.8 10.0 9.'8 6.4 8.8 9.0 9.0 9.2 9.3 9.4 9.3 9.6 9.7 9.8 9.8 9.9 10.0 6.5 6.3 6.5 6.7 6.8 7.0 6.9 7. 1 7.4 7.1 7.4 7.3 7.6 7.5 6.0 6.2 6.5 6.6 6.7 6.7 6.9 6.7 7.1 7.3 7.2 7. 1 7.3 7.2 5.8 7.2 7.5 7.7 7.9 7.9 8.1 8.3 8.4 8.4 8.2 8.3 8.4 8.6 6.0 6.3 6.4 6.6 6.7 7.0 7.2 7.0 7.4 7.4 7.4 7.7 7.6 7.6 6. 1 6.5 6.7 6.9 7.0 7.2 7.3 7.3 7.6 7.6 7.6 7.6 7.7 7.7 6.5 6.9 6.9 6.8 7.0 6.9 7. 1 7.0 7.1 7.2 7.3 7. 1 7.3 7. 1 6.5 7.4 7.8 8.0 8. 1 8. 1 8.5 8.3 8.6 8.6 8.7 8.9 9.0 9.0 6. 5 5.9 5.8 6.0 6. 1 6.2 6. 2 6.2 6.4 6.3 6.4 6.5 6. 5 6.5 5.8 7.4 7.5 7.7 8.0 8.2 8.6 8.7 9. 1 9.4 9.3 9.4 9.9 9.9 6.3 6.9 7.0 7. 1 7.3 7.4 7.6 7.6 7.8 7.9 7.9 8.0 8. 2 8. 1 is a i-o ifl r TABLE III continued TEST MATERIAL: Aroclor 1254 Two-Year Chronic Oral Toxicity Study - Beagle Doga Body Weight Data for Females, kilograms BI 0 - T E ST J a iM a k tia i, Pttc. LEXOLDMON002862 Group Dietary Level (ppm) Dog Number UC TJone 5 6 7 8 Mean I 1 13 14 15 16 Mean II 10 21 22 23 24 Mean III CCOD za o IS) tn 00 NJ 100 29 30 31 32 Mean Body Weights at Week Indicated: 13 14 15 16 11____L8____Li___ 20___ U____22___ 23__-24_.. 25. __ 26_ 6.2 6.5 7.0 7.3 7.5 7.8 8.0 7.9 8. 1 8. 1 8.2 8.3 8.3 8.1 9.0 9.0 9. 1 9.1 9.3 9. 1 9.3 9.5 9.5 9.4 9.6 9.4 9.5 9.5 7.9 8.2 8.2 8.3 8.3 8.5 8.8 8.5 8.9 8.7 8.9 8.7 8.7 8.8 9.9 10. 1 10.2 10. 3 10.4 10. 5 10. 5 10.2 10.4 10.4 10.9 10.9 10.9 10.9 8.2 8.4 8.6 8.8 8.9 9.0 9.2 9.0 9.2 9.2 9.4 9.3 9.4 9.3 9.4 9.6 9.0 8.6 8.4 8.3 8.9 9.0 8.6 8.9 9.2 9.5 9.2 9. 1 9.8 10.1 9,8 10.0 9.7 9.8 10. 1 10.0 10.4 10.0 10.5 10.5 10.2 10.1 10.7 11.0 10.6 10.8 10.6 10. 5 11. 1 10.9 11.1 11.0 11.4 11. 1 11. 1 11.0 9.5 9.7 9.8 9.9 10.2 10.4 10.7 10.6 10.9 10.6 10.9 11. 1 11.0 11.0 9.9 10.1 9.8 9.8 9.7 9.8 10.2 10.1 10.2 10.1 10. 5 10.6 10.4 10.3 7.4 7.7 7.8 8.0 8.0 7.8 8.0 7.9 8.1 8.0 8.1 7.9 8.6 8.0 6.7 7.0 7. 1 7.2 7.2 7.4 7.6 7.4 7.6 7.6 7.8 7.6 8. 1 7.9 8.2 8.4 8. 5 8.5 8.5 8.5 8.6 8.7 8.6 8.6 8.5 8.5 8. 5 8.5 7. 1 7.6 7.5 7.6 7.5 7.7 7.9 7.8 7.9 7.7 8.0 7.8 8.3 7.8 7.4 7.7 7.7 7.8 7.8 7.8 8.0 8.0 8.0 8.0 8.2 8.0 8.4 8.0 7.1 7.2 7. 3 7.4 7.3 ,7.6 ' 7.6 6.7 7.6 7.5 7.3 7.6 7.8 7.6 9. 1 9.3 9.3 9.6 9.4 9.4 9.4 9.5 9.4 9.0 9.3 9.4 9.5 9.4 6.5 6.7 6. 5 6.7 6.8 6.6 6.7 6.9 7.0 6.8 7.0 7. I 7. 1 6.9 9.7 9.6 9.7 9.8 9.9 10.2 10. 5 10.6 10.7 10.5 10.7 10.8 10. 9 10.7 8. 1 8.2 8.2 8.4 8.3 8. 4 8.6 8.4 8.7 8.4 8.6 8.7 8.8 8.6 I S 3 1 " 0 1 9 TWl*"P?(i TABLE III continued TEST MATERIAL: Aroclor 1254 Two-Year Chronic Oral Toxicity Study - Beagle Dogs Body Weight Data for Females, kilograms Group UC 1 II III ctfnl a Z o 0 N 01 t0.0I Dietary Level (ppm) Dog Number None 5 6 7 8 Mean 1 13 14 15 16 Mean 10 21 22 23* 24 Mean 100 29 30 3 j ** 32 Mean Body Weights at Week Indicated: 27 28 2? ?9 31 32 -33- _15--.. 36- 37_-33- ..39-- -4Q_ 8.3 9.5 8.8 11.0 9.4 8.3 9.5 8.9 10.9 9.4 8.3 9.5 9.2 10.9 9. 5 8.4 9.8 9. 1 11.0 9.6 8. 5 9.8 9.2 10.8 9.5 8.6 9.8 9.3 10.4 9. 5 8.5 9.4 9.2 10.8 9.5 8.5 9.5 9. 1 10.8 9.5 8.9 9.9 9.3 10.6 9.7 8.5 9.6 9.3 10.5 9.5 8.4 9.7 9.2 10.4 9.4 8.4 9.6 9.0 10.4 9.4 8.6 9.9 9.0 10.7 9.6 8. 5 10;0 9. 1 10.6 9.6 9.1 9.2 8.7 8.7 8.7 8.8 8.7 8.3 8.3 9.0 8. 5 8.6 . 8.6 9.0 10.2 10.4 10.0 10. 5 10.4 10.2 9.8 9.5 9.4 9.6 10.2 9.6 9.7 9. 5 10.9 10.8 11.0 11. 1 11.0 10.6 11.2 11.4 11.6 11.8 11.6 11. 1 11.2 10.8 11. 1 11. 1 10. 9 11. 1 11.0 11.0 10.9 11.3 11.3 12.0 11.9 11.2 11.7 11.6 10.3 10.4 10. 2 10.4 10.3 10.2 10.2 10. 1 10.2 10.6 10.6 10. 1 10. 3 10. 2 8. 1 8. 1 8. 2 8. I 8.0 8.0 7.9 8.0 8.4 8.5 8.3 8.2 8.3 8.3 7.2 7.3 7. 5 7.4 7.4 7. 5 7.4 7.5 7.6 7.7 7. 5 7.4 7.4 7. 5 8.6 8.6 8. 5 8.3 8.3 8. 3 8.3 8.5 8.0 6.8 5.8 - - 8.0 8.0 8. 5 7.9 7.9 8.0 8.0 8.0 8.2 8.2 8. 1 8. 1 8. 1 8. 2 8.0 8.0 8.2 7.9 7.9 8.0 7.9 8.0 8.0 7.8 7.4 7.9 7.9 8.0 7.6 9.4 6.8 10. 5 8.6 7.6 9. 5 6.6 10. 5 8.6 7. 5 9.4 6. 5 10. 5 8. 5 7.8 9.4 6. 5 10.6 8.6 7.4 7. 3 ` 7.3 9.4 ' 9.3 9.2 6. 4 6. 4 6.0 10. 5 10. 3 10. 3 8.4 8. 3 8.2 7.2 9.2 - 10.3 8.9 7.5 9.4 - 10. 5 9. 1 7.5 9.6 - 10.8 9.3 7. 5 9. 5 - 10. 7 9. 2 7.3 9.5 - 10.8 9.2 7.4 9.6 - 10.7 9.2 7. 3 9.6 - 10.8 9. 2 LEXOLDMON002863 Utibual B l 0 - T EST JaUtattvuel. Stic. LEXOLDMON002864 TABLE III continued TEST MATERIAL: Aroclor 1254 Two- Year Chronic Oral Toxicity Study - Beagle Dogs Body Weight Data for Females, kilograms Dietary Level Dog Body Weights at Week Indicated e Group (ppm) Number 41 42 43 44 45 46 47 48 49 50 51 52 53 54 uc None 5 9.0 8.7 8.7 8.6 8.6 8.6 8.8 9.0 9.2 8.9 9.2 9.0 9.0 9.0 6 10.7 10.4 10.4 10.3 10. 1 10.0 10. 1 10.2 10.3 9.8 10.0 9.7 9.7 9.7 7 9.5 9. 1 9.3 9.2 8.8 8.9 9.2 9.4 9.3 9.0 9.2 9.4 9.5 9.3 8 11.2 11.0 11.2 11.2 11. 1 10.8 11.4 11.5 11.8 11.1 11.5 11.5 11.7 11.9 Mean 10. 1 9.8 9.9 9.8 9.6 9.6 9.9 10.0 10.2 9.7 10.0 9.9 10.0 10.0 I. 1 13 9.3 9.6 10.2 10.2 9.5 9.5 10. 1 10.3 10.1 10.2 10.5 10.8 11.3 11.2 14 9.8 10.2 10. 1 10.2 9.9 9.9 10.0 10.2 10.7 10.5 10.4 10.9 10.9 10.6 15 11.0 10.9 11. 1 11. 1 10.9 11.0 11.0 11.2 11.3 11. 1 11.4 11.3 11.5 11.4 16 11.4 12.0 11.9 11.8 11.7 11.5 11. 5 11.4 11.8 11.5 11.5 11.7 11.6 11.6 Mean 10.4 10.7 10.8 10.8 10. 5 10.5 10.6 10.8 10.9 10.8 11.0 11.2 11.3 11.2 II 10 21 8.5 8.5 8.9 8.8 8.6 8.5 8.9 9. 1 9.1 8.9 9.2 9.0 8.7 8.6 22 7.5 7.5 7.7 7.7 7.7 7.6 7.7 7.7 7.8 7.6 7.6 7.6 7.6 7.7 23* 24 8.2 8. 1 8.2 8. 1 7.9 7.9 8. 1 8.2 8.3 8.1 8.2 8. 1 8.2 8.8 Mean 8. 1 8.0 8.3 8.2 8. 1 8.0 8.2 8.3 8.4 8.2 8.3 8.2 8.2 8.4 III 100 29 7.5 7.4 7<6 7.6 7.4 .7-4 '7.5 7.7 8.0 7.8 7.9 7.8 7.9 7.9 30 9.7 9.8 10.0 9.8 9. 5 9. 5 9.7 9.7 9.8 9.5 9.5 9.5 9.6 9.5 31** - - - - - - - - - - - - 32 10.9 11.0 11. 1 11. 1 11.0 11. 1 11.4 11.3 11.6 11.4 11.3 11. 3 11.6 11. 3 Mean 9.4 9.4 9.6 9. 5 9.3 9.3 9.5 9.6 9.8 9.6 9.6 9. 5 9. 7 9.6 isai-oia rw r* TABLE III continued TEST MATERIAL: Arocior 1254 Two-Year Chronic Oral Toxicity Study - Beagle Doga Body Weight Data for Females, kilograms Dietary Level Group (ppm) Dog Number 55 Body Weights at Week Indicated: 56 57 58 59 60 61 62 63 64 65 66 67 68 UC None 5 8.6 9. 1 8.9 8.8 8.7 8.5 8.8 8.5 8.9 8.7 8.7 8.6 8.8 8.9 6 9.5 9.5 9.4 9.6 9.6 9.7 9.7 9.6 9.8 9.8 9.8 9.9 10. 1 10. 1 7 8.9 9.3 9.6 9.4 9.7 9.9 9.9 9.8 9.7 10.0 9.9 10.0 9.9 9.6 8 11.7 11.9 11.9 11.9 12. 1 12. 1 12.3 11.9 11.8 11.5 11.4 11.2 11.9 11.9 Mean 9.7 10.0 10.0 9.9 10.0 10.0 10.2 10.0 10.0 10.0 10.0 9.9 10.2 10. 1 I 1 13 11.6 12. 1 12.0 12.7 12.4 13.0 13.2 13.3 13.3 13.2 13. 1 13. 1 13.0 12.8 14 10.5 10.8 10.7 10.7 10.7 10.6 10.7 10.7 11.0 11. 1 11.4 11.4 11.1 11.5 15 11.3 11.5 11.6 11.7 11.8 11.8 11.9 11.9 12. 1 12.2 12.2 12.4 12.5 12.6 16 11.6 12. 1 11.8 11.6 11.8 11.6 12.0 11.8 12.0 12.3 12.2 12.5 12.4 12.3 Mean 11.2 11.6 11.5 11.7 11.7 11.8 12.0 11.9 12. 1 12.2 12.2 12.4 12.4 12.3 II 10 21 8.9 8.8 8.9 8.7 8.8 8.8 8.9 9. 1 9.0 9.0 8.8 8.9 9.1 9.0 22 7.6 7.4 7.6 7.6 7.6 7.6 7.5 7.5 7.4 7.5 7.5 7.7 7.7 7.6 23* . 24 8.0 7.8 7.9 7.8 7.8 7.8 7.9 7.6 8.0 8.0 7.9 8.0 8.3 8.2 Mean 8.2 8.0 8. 1 8.0 8. 1 8. 1 8. 1 8. 1 8. 1 8.2 8. 1 8.2 8.4 8. 3 III 100 29 7.8 7.9 7.7 7.6 7.4 7.2 7.3 7.5 7.4 7.4 7.3 7.6 7.5 7.5 30 9.5 9.6 9.5 9.7 9.7 9.7 9.8 9.8 9.8 9.8 9.8 9.8 10.2 10. 1 31** - 32 11.2 11.2 11.0 11. 1 11.0 10.9 10.7 10.7 10.7 10.4 10.5 10.8 10.7 11.0 Mean 9.5 9.6 9.4 9.5 9.4 9. 3 9.3 9.3 9.3 9.2 9.2 9.4 9.5 9.5 LEXOLDMON002865 LEXOLDMON002866 % TABLE HI continued TEST MATERIAL: Aroclor 1254 Two-Year Chronic Oral Toxicity Study - Beagle Dogs Body Weight Data for Females, kilograms Dietary Level Group (ppm) Dog Number 69 Body Weights at Week Indicated 70 71 72 73 74 75 76 77 78 79 80 81 82 UC None 5 8.6 8.8 8.9 9.0 9.2 9. 1 9. 1 9.0 9.0 9.2 9.3 9.2 8.9 9.4 6 10. 3 10.4 10.3 10.3 10.5 10.7 10.4 10.3 10.3 10.5 10.6 10.0 10.2 10.0 7 9.6 9.7 9.5 9.4 9.5 9.4 9.5 9.4 9.2 9.4 9.3 9.0 9.2 9.4 8 11.8 12. 1 12.0 11.6 12.2 11.9 12.0 12. 1 12.3 12. 1 12.2 11.9 12.2 12.2 Mean 10. 1 10.2 10.2 10. 1 10.4 10. 3 10. 2 10.2 10.2 10.3 10.4 10.0 10.1 10.2 I 1 13 11.8 12.4 12.4 12.7 12.9 12.9 12.5 12.6 12.6 12.6 12.6 12.2 12.6 12.5 14 11.5. 11.8 11.9 11.5 11.8 11.6 11.4 11.5 11. 1 11.4 11.2 10.6 11.2 11.1 15 12.5 12.6 12.7 12.5 12.7 12. 3 12. 1 12.4 12.0 12.4 12.4 12.2 12.3 12.2 16 12. 1 12.2 12.4 12.4 12.0 11.9 11.8 12. 1 11.9 12.5 12.2 12.0 12. 2 12.2 Mean 12.0 12.2 12.4 12.3 12.4 12.2 12.0 12.2 11.9 12.2 12. 1 11.8 12.1 12.0 II 10 21 9.2 9.0 9.0 9.0 9.2 9. 1 9.0 9.0 9. 1 9.0 9.2 9.1 9.2 9.3 22 7.8 8.0 7.8 8.0 8.0 8. 1 8.2 8. 1 8. 1 7.9 8.0 7.9 7.6 7.5 23* 24 8.2 8.5 8.3 8.2 8.2 8.0 7.9 7.9 7.9 7.9 8.1 7.9 8.1 8.1 Mean 8.4 8.5 8.4 8.4 8.5 8.4 8.4 8.3 8.4 8.3 8.4 8.4 8.3 8.3 IQ 100 29 30 31** 32 Mean 7.6 7.6 7.6 7.5 7.6 7.5 7.3 7.5 7.6 7.5 7.6 7.5 7.6 7.6 10. 1 10.4 10.2 10.0 10.2 It). 1 9.8 9.9 9.7 9.8 9.8 9.7 9.3 9.6 J0i3. 11.0 11.0 10.7 11.0 10.8 10.8 11. 1 11. 1 11. 1 11.3 10.9 11.2 11.2 9.5 9.7 9.6 9.4 9.6 9.5 9. 3 9.5 9.5 9.5 9.5 9.4 9.4 9.5 1 S31-O I9 r w v - NM LEXOLDMON002867 TABLE III continued TEST MATERIAL: Aroclor 1254 Two-Year Chronic Oral Toxicity Study - Beagle Doge Body Weight Data for Females, kilograms Group Dietary Level (PPm) UC N.me I1 II 10 III 100 Dog Number 5 6 7 8 Mean 13 14 15 16 Mean 21 22 23* 24 Mean 29 30 31** 32 Mean Body Weights at Week Indicated: 83 84 85 86 87 88 89 90 91 ?2 V 94 95 9. 1 9.4 9.9 10.0 9.8 9.7 9.7 9.6 9.9 9.5 9.5 9.2 9.2 10.0 10.3 9.6 10. 1 10. 1 10. 1 10.5 10.5 11.0 10.1 10.5 10.5 10.5 9.5 9.9 10.0 10.0 10.0 9.9 10.1 10. 2- 10.9 10.0 9.9 9.7 10.0 12. 2 12.6 12.5 12.8 12. 5 12.3 12.7 12.9 13.0 12.5 11.7 12.4 12.4 10.2 10.6 10.5 10.7 10.6 10.5 10.8 10.8 11.0 10.5 10.4 10.4 10.5 12.5 12.5 12.4 12.6 12.6 12.8 13.0 13.5 14.0 13.5 13.7 13.7 13.9 11.2 11. 1 10.9 11.2 11.0 11.0 10.8 10.8 11.5 11.3 11.5 11.5 11.5 12.4 12.4 12.6 13.0 13.0 13.0 12.9 13.0 13.2 13.1 13.0 13.0 13.5 12.2 12.3 12.4 12.7 12.4 12. 2 12.8 13.9 13. 2 13.9 13.0 13.0 13.0 12. 1 12. 1 12. 1 12.4 12. 2 12. 2 12.4 12.6 13.0 12.7 12.8 12.8 13.0 9.2 9.2 9.2 9.4 9.2 8.9 9.0 8.9 9.0 8.8 8.9 8.7 8.7 7.5 7.5 7.6 7.8 8.0 8.0 8.0 8.0 8.0 7.5 7.8 7.8 7.7 - 8.3 8. 2 8.5 8.3 8.3 8.3 8.1 8.5 8.5 8.2 8.2 8.2 8.7 8.3 8.3 8.4 8.5 8. 5 8.4 8.4 8.5 8.5 8.2 8.3 8.2 8.4 7.6 7.8 7.7 7.9 ,7.7 ' 7.4 7.4 7.5 7.6 7.4 7.5 7.5 7.8 9.7 9.6 9.6 9.9 9.9 9.9 10.0 10.4 10.5 10.3 10. 2 10.2 10.2 11.2 11.3 11. 2 11.2 11. 1 11.0 10.8 10.8 10.6 10.8 10.7 11. 2 11. 1 9.5 9.6 9.5 9.7 9.6 9.4 9.4 9.6 9.6 9.5 9.5 9.6 9.7 O m M -* NUl B IO -T E S T LEXOLDMON002868 G) CO TABLE III continued 30 oZ o TEST MATERIAL: Aroclor 1254 l\> U CO Two-Year Chronic Oral Toxicity Study - Beagle Dogs 00 Body Weight Data for Females, kilograms Group Dietary Level (PPm) Dog Number Body Weights at Week Indicated: 96 97 98 99 100 101 102 103 104 Overall Weight Gain UC None 5 6 7 8 Mean 9.2 9.0 8.9 9.2 9.3 9.0 9.3 9.1 9.2 10.4 10.3 10.5 10.3 10.3 10.4 10.6 10.4 10.6 9.9 9.5 9.7 9.3 9.5 9.2 9.6 9.4 9.5 12.2 12.4 12.5 12.5 12.6 12.5 12.4 12.4 12.5 10.4 10.3 10.4 10.3 10.4 10.3 10.4 10.3 10.4 2.8 3.5 3.0 5.3 3. 1 I 1 13 13.7 13. 5 13.5 13.3 13. 1 12.9 13.1 13.3 13.1 14 11.5 11. 2 11.5 11.6 11.5 11.1 11.4 11.5 11.6 15 13.4 13. 1 13.2 13.2 13.3 13.2 13.0 12.8 13.1 16 12.9 12.8 12.8 13.0 12.8 12.5 12.3 12.7 12.6 Mean 12.9 12.6 12.8 12.8 12.7 12.4 12.4 12.6 12.6 4.8 3.9 3.8 3.9 3.8 II 10 21 8.7 9.0 8.7 8.9 8.6 8.6 8.7 8.8 8.9 22 7.1 6.5 6.3 6.6 6.8 7.1 7.3 7.5 7.8 23* - -----A-- 24 8.5 8.4 8.2 8.0 8. 1 8.0 8.2 8.3 8.4 Mean 8. 1 8.0 7.7 7.8 7.8 7.9 8. 1 8.2 8.4 2.6 1.6 (-1.4) 2.1 2.1 III 100 29 7.8 7.6 7.5 7.8 7.6 7.4 7.6 7.7 7.6 30 10. 2 10. 2 10.2 10.0 10. 1 9.8 10.9 10. 1 10.0 31** - - ---- - -- 32 11.3 11.4 11.3 11.6 11. 3 11.0 11. 1 11.3 11. 1 Mean 9.8 9.7 9.7 9.8 9.7 9.4 9.6 9.7 9.6 0.7 2.6 (0. 1) 3.2 2.4 * animal died after 37 weeks of testing. ** animal died after 33 weeks of testing. Note; Overall weight gains for animals that died during the study are in parentheses and are not 5,,^|...l ..1 J .. st... - - r $wiMihlal BIO-TEST J.al*4aic*imL, Hmc. 25 B. Food Consumption Data Food consumption data are presented in Table IV. No significant abnormalities were noted at any of the levels tested. SBRN0025B9 jfo/wAAt/ BIO-TEST 2aioaio*iU, TABLE IV 26 TEST MATERIAL: Aroelor 1254 Two-Year Chronic Oral Toxicity Study - Beagle Dogs Food Consumption Data Week Sex: Group: Dietary Level (ppm): Mean Food Consumed During Week Indicated (gm/kg of body weight) Males Females UC I II III UC I II III None 1 10 100 None 1 10 100 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 ` 20 21 22 23 24 25 26 27 268 238 256 272 282 258 284 261 290 260 308 268 277 247 272 268 287 269 298 223 290 244 296 243 305 255 273 212 276 247 257 257 285 243 281 196 279 239 250 260 323 252 333 274 348 322 339 336 201 197 209 182 180 185 174 135 280 261 287 187 274 276 256 244 244 231 171 293 300 297 387 192 253 252 276 208 262 250* 266 272 244 210 243 167 209 247 227 213 Z61 254 172 220 265 268 -250 241 267 218 223 164 238 232 199 231 296 231 257 184 235 260 214 238 253 229 240 176 276 232 204 232 287 244 257 203 304 224 266 247 253 244 233 219 250 209 244 229 286 242 234 250 306 247 247 2 62 278 266 204 263 293 270 272 247 237 232 159 237 269 260 266 286 242 242 245 266 282 289 259 253 234 222 243 247 264 265 244 238 282 234 273 250 279 260 268 262 250 233 252 228 255 243 219 267 . 235 223 259 316 253 197 289 224 245 244 239 228 372 218 212 232 245 236 219 231 316 238 259 227 GBRN002590 LEXOLDMON002870 Mttihlnl BIO-TEST Sac. 27 TABLE IV continued TEST MATERIAL: Aroclor 1254 Two-Year Chronic Oral Toxicity Study - Beagle Dogs Food Consumption Data Week Sex: Group: Dietary Level (ppm): Mean Food Consumed During Week Indicated (gm/kg of body weight) Males Females UC I n m UC I n HI None l 10 100 None 1 10 100 28 300 244 270 271 292 243 278 267 29 262 227 243 238 266 245 229 238 30 252 240 241 269 281 260 270 256 31 260 221 248 269 281 240 266 256 32 332 280 279 317 368 274 351 376 33 214 245 279 277 239 263 240 235 34 254 266 275 272 308 277 303 277 35 282 269 297 253 286 279 256 297 36 260 213 183 250 237 278 199 240 37 252 234 224 238 271 229 206 261 38 248 238 242 229 260 205 262 254 39 255 264 239 259 258 240 ' 266 250 40 216 232 207 211 281 252 238 228 41 267 216 254 207 287 267 272 294 42 267 236 248 232 258 280 262 259 43 255 267 250 253 270 280 253 281 44 238 256 248 259 255 278 248 235 45 ' 292 246 248 277 271 257 280 283 46 243 229 233 241 263 255 229 244 47 238 259 242 223 252 257 240 238 48 250 242 227 222 260 268 237 236 49 231 220 2rl5 198 228 261 182 238 50 235 205 221 212 191 208 228 222 51 226 213 196 226 245 223 233 243 52 212 241 222 229 225 241 220 249 53 ' 250 217 203 217 235 212 203 247 54 233 202 207 210 232 208 218 260 BBRN002591 LEXOLDMON002871 faiudhial BIO-TEST Smc. 28 TABLE IV continued TEST MATERIAL: Aroclor 1254 Two-Year Chronic Oral Toxicity Study - Beagle Dogs Food Consumption Data Week Sex: Group: Dietary Level (ppm): Mean Food Consumed During Week Indicated (gm/kg of body weight) Males Females UC I II m UC I II in None 1 10 100 None 1 10 100 55 168 196 181 198 209 210 183 179 56 224 197 209 220 232 244 208 222 57 238 186 215 238 228 270 222 238 58 208 185 187 208 217 233 200 214 59 233 206 212 220 220 246 214 245 60 205 212 181 211 222 218 198 222 61 215 210 195 220 211 231 214 222 62 178 188 143 176 188 202 189 201 63 193 219 183 195 208 233 226 236 64 212 168 188 205 225 232 220 194 65 186 227 185 195 212 218 206 236 66 208 213 201 202 212 192 220 227 67 222 207 212 222 220 206 210 232 68 217 193 195 198 213 197 201 210 69 215 224 199 232 208 156 214 235 70 231 200 200 247 218 231 22 0 261 71 206 192 194 196 198 232 218 245 72 . 204 194 195 203 220 187 198 213 73 210 219 204 226 226 175 204 229 74 229 215 204 223 216 199 198 204 75 224 235 188 256 208 167 167 158 76 222 213 206 202 211 217 237 256 77 229 239 208 244 233 206 226 260 78 204 258 185 304 250 236 301 200 79 219 239 226 253 243 248 230 2 46 6BRN002592 LEXOLDMON002872 fafatltfal BIO-TEST Jaio*a(\in, Sue. 29 TABLE IV continued TEST MATERIAL: Arodor 1254 Two-Year Chronic Oral Toxicity Study - Beagle Dogs Food Consumption Data Week Sex: Group: Dietary Level (ppm): Mean Food Consumed During Week Indicated (gm/kg of body weight) Males Females UC I None 1 II III UC I 10 100 None 1 II III 10 100 .80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 Mean 240 244 236 231 222 265 246 252 262 261 238 265 235 248 289 266 277 246 219 277 235 223 . 273 256 274 247 254 262 233 248 261 263 265 237 224 250 233 244 277 236 286 231 236 259 257 236 258 281 300 250 248 259 243 242 274 271 262 205 217 199 262 217 216 205 250 231 232 235 226 211' 230 273 231 205 197 229 248 206 238 227 264 241 234 253 243 214 -216 264 261 228 199 220 204 210 167 229 191 202 181 220 171 183 154 210 224 230 194 235 161 189 193 256 259 236 220 232 195 223 203 271 229 232 204 235 200 221 206 271 211 208 202 229 183 205 177 252 222 193 212 244 214 192 188 258 224 228 208 247 209 211 177 251 224 236 217 265 218 205 235 265 243 212 205 241 219 181 226 236 221 214 199 214 209 181 219 213 236 221 196 246 213 196 198 258 228 217 208 235 217 207 213 261 241 226 213 238 191 213 217 248 246 231 227 235 246 236 232 247 GBRN002593 LEXOLDMON002873 ItoditihiaL BIO T E S T 2a&e*g((*i*d, Hue. 30 C. Reactions No abnormal behavioral reactions were noted at any of the levels tested. D. Mortality There were two fatalities during the study, a female (no. 31) receiving 100 ppm died after 33 weeks of testing and a female (no. 23) receiving 10 ppm died after 37 weeks. In the 100 ppm animal, the cause of death was attributed to severe traumatic injuries sustained in a fight. Histologic examination of the tissues from this dog revealed no abnor malities. In the 10 ppm animal, gross and histopathologic examinations of the tissues revealed a moderate chronic peritonitis. . No other fatalities occurred during the study. ^ E. Hematologic Studies The results of these determinations are presented in Tables V through IX. Dashes in the tables indicate animals that died during the study. No significant abnormalities were noted at any of the levels tested. The high total leukocyte counts noted frequently among test group HI animals (100 ppm Aroclor 1254) are not considered a re sponse to test material ingestion since the values are within the upper limits of the normal range in this laboratory. BBRN002594 LEXOLDMON002874 Stultiibial B I O T E S T 2aiiata\ia4, !)*&. 31 TABLE V . TEST MATERIAL: Aroclor 1254 Two-Year Chronic Oral Toxicity Study - Beagle Dogs Hematologic Studies: Total Leukocyte Count, thousands/mm ^ Group UC I II HI . Dietary Level (ppm) None 1 10 100 Dog Number and Sex 1-M 2-M 3-M 4-M 5-F 6-F 7-F 8-F 9-M 10-M 11-M 12-M 13-F 14-F 15-F 16-F 17-M 18-M 19-M 20-M 21-F 22-F 23-F 24-F 25-M 26-M 27- M 28-M 29-F 30-F 31-F 32-F Months: 0 1 3 6 12 18 14.7 18.3 9.4 18.8 15.4 11.6 21.7 12.0 13.6 19.7 15.6 21.2 13.7 16. 5 18.1 14.7 12.7 19.4 11.0 15.5 27.6 14.1 10.3 10.8 12.9 19. 1 10.5 13.6 22.0 18.7 17.7 19.7 15. 5 19.0 11.6 15.2 11. 5 16.6 15. 5 16.0 9.2 16.3 13.2 12.4 9.5 14.6 11.4 10.8 10.5 7.0 10.9 15.4 13.6 12.8 12.2 14.5 18.2 10.9 11.7 17.3 15.9 12.3 11. 1 H.5 12.8 11.7 11.8 17.9 16.7 13.0 12.1 14.0 9.4 9.9 12.0 15.3 12.4 15. 1 17.4 14. 1 11.6 12.2 12.6 20.8 21. 1 16.6 11.3 15.8 12.6 12.4 13.6 16.9 15.7 16.7 10.8 12.7 11.9 11.7 13. 5 9.0 14.8 13.9 13.7 12.6 13.2 17.6 19.4 13.5 17.4 21.5 16.7 24.8 14.7 15.2 15.3 14.0 21.9 26.3 22.0 13.9 15.8 16.4 15.2 14.4 13.5 14.0 15.8 12.5 16.2 17. 1 14.2 12.9 22.8 15.5 - 11.8 15.0 25.8 15.8 13.3 10.2 11.8 10.8 16.3 23.3 16. 5 15.3 13.0 13. 1 13.3 26.4 19.4 29.8 22.9 18.9 24.8 24.4 24.6 21.7 17.9 18.3 N/A 15.2 17.5 22.7 18.0 15.6 19.2 22.6 23.9 14.2 14.0 17.0 17.0 13.4 25.6 28.3 27.0 24.3 17.5 17.3 - 17. 5 17.8 25. 1 24. 1 22. 1 20.7 16.8 . 15.3 24 10.4 14.0 9.1 11.9 10.6 12.4 10.6 12.6 11.5 15.0 13.6 15. 5 14.7 16. 2 14. 8 12.8 14. 2 16.9 18.4 13.0. 12.9 12.9 11.0 19.8 29. 1 24.6 19.4 17.0 19.7 14.5 N/A - data not available BBRND02595 LEXOLDMON002875 jtUuii%ial B I 0 T E S T Jniawtwiisi, 2*c. 32 TABLE VI TEST MATERIAL: Aroclor 1254 Two-Year Chronic Oral Toxicity Study .- Beagle Dogs Hematologic Studies: Erythrocyte Count, millions /mm3 Dietary Dog Level Number Months: Group (ppm) and Sex 0 1 3 6 12 18 24 UC None 1-M 6.09 6.73 6.80 6.35 7.92 7.21 6.78 2-M 5.69 5.76 6.40 6.45 8.37 6.69 7. 10 3-M 5.96 6. 14 5.76 6. 95 8.13 6.68 7. 22 4-M 5.83 5.98 5.32 6. 11 7.44 7. 13 7. 16 5-F 5.23 6.05 5. 50 7.23 8.36 6.84 6.58 6-F 5.41 5.89 6.04 6.63 7.42 6.27 6.71 7-F 5.48 5.95 6.08 6.93 7.61 7.08 6. 75 8-F 5.43 5.86 6.35 6.67 7.33 7. 12 6.72 I 1 9-M 6. 51 6.41 6.65 6.88 7.44 6.63 6.99 10-M 5. 10 5.35 5.45 6.11 6.54 6.34 6.62 11-M 5.74 5.61 5.98 6. 58 6.97 6.88 6.54 12-M 5.51 5. 93 6.23 6.65 7.06 7.05 7.05 13-F 5.66 6.03 6.05 6.24 6.67 6. 52 5. 55 14-F 5.84 6.48 6.50 6. 50 7.80 6.70 6.05 15-F 5.68 6.05 6.23 6.03 7.64 6.43 6. 14 16-F 6. 16 6.23 6.54 6.60 7.96 7.25 7.58 II 10 m. 100 17-M 18-M 19-M 20-M 21-F 22-F 23-F 24-F 25-M 26-M 27-M 28-M 29-F 30-F 31-F 32-F 5.23 6.18 5.71 6.09 6.29 5.45 5.68 5.86 5.46 5. 80 5.64 5.24 7.08 5. 83 6.24 6.48 5.36 5.78 5.78 6.29 6.83 5.18 5.75 6.31 5. 86 6.09 6. 56 6.35 6.78 6.43 6.30 6.34 6.53 7.15 6.83 7.10 7.99 7.31 - 7.85 5.96 5.60 5.21 6.00 5.84 5.83 6.81 5.50 5.63 4. 90 . 4.69 5.88 6.44 6.34 7. 15 6.28 5.64 5.61 N/A 6.01 6.01 6.36 6.96 6.18 6. 14 6.20 6. 14 6.90 5.89 6.01 6.76 6.82 5. 10 6.20 6.21 6.67 7.15 6.91 7.99 6.61 7.04 6.90 6.91 7.40 6.45 . 6.46 6.30 6.83 6.86 6.90 7.40 6. 27 - 6.79 6.07 6. 13 6.30 6.34 5.93 6.28 - 6.92 6.66 6.19 6.05 6.77 6.54 5.88 - 7.37 N/A - data not available GBRN002596 LEXOLDMON002876 B I O * T E 5 TJ.tiiual Jai&afouoi, Une. 33 TABLE VU TEST MATERIAL: Aroclor 1254 Two-Year Chronic Oral Toxicity Study - Beagle Dogs Hematologic Studies: Hemoglobin, gm/100 ml Dietary Dog Level Number Months: Group (ppm) and Sex 0 1 3 6 12 18 24 UC None 1-M 15.8 16.7 17. 5 16.2 17.6 17.6 16.4 2-M 13.2 13.0 14.6 13.8 15.8 15.2 15.7 3-M 14.4 12.9 14.3 16.2 16.6 15.3- 16. 1 4-M 14.3 12.9 13.0 14.9 16.4 17.2 16.9 5-F 14.8 15.8 13.6 17.7 17. 3 16.8 16. 3 6-F 14.4 14.2 14.7 15.9 15. 1 15.5 15.7 7-F 13.8 13.5 14.8 15.9 15.3 16.5 15.7 8-F 13.8 14. 1 14.6 15.9 14.9 16.6 15.8 I 1 9-M 18.2 15.9 17.4 17.7 16.0 16.5 16.9 10-M 15.4 14.7 14.6 15.4 13.7 15.4 15.9 11-M 14.2 14.0 14.4 15.8 14.3 17.0 15.5 12-M 14.0 14.7 15.4 16.0 15.2 16.8 16.9 13-F 14.6 14.7 15.4 16.5 . 14. 1 15.5 13.7 14-F 15.0 15.4 16.6 17. 1 16.8 17. 1 14.4 15-F 15.2 14.9 16.9 16.0 17.4 16.5 15. 3 16-F 15.3 16.5 17.0 17.7 16. 9 17.7 18. 2 II 10 17-M 12.4 12.9 13.4 14.9 14.3 16.3 15.7 18-M 16. 1 14.2 14.2 14.2 15. 1 16.3 15. 7 19-M 15.4 13. 1 15.0 16.5 14.5 16.8 16. 4 20-M 15.4 13.9 14.8 16.9 16.0 17.0 16. 7 21-F 16.0 18.1 17.0 17.6 16.8 18.4 17.8 22-F 13.8 14.2 13.0 16.3 16. 1 16.5 15.4 23-F 14.8 15.2 14.6 15.3 - -- 24-F 14.6 14.5 15.9 15.9 16. 5 15.7 15.8 in 100 25-M 13.9 13.4 16.4 14.6 10.6 15.3 15.7 26-M 13.0 11.9 14.0 15.8 13.5 15.6 14. 8 27-M 13. 5 11.9 N/A 16.0 14.0 15. 5 14.7 28-M 13.7 13.7 15.0 16.6 14.3 15. 1 16.0 29-F 14.7 15.4 14.6 14.3 15.4 14.4 15. 2 30-F . 14.8 15.8 16.2 15.5 15.3 16.8 14. 8 31-F 16.8 17.5 18.4 17.2 - m- 32-F 15.6 15.2 16.2 17.5 18.0 17.3 17. 8 N/A - data not available GBRND02597 LEXOLDMON002877 'faituUial BIO-TEST ^<\in\n(atiaS, Sue. 34 TABLE VIII. TEST MATERIAL: Aroclor 1254 Two-Year Chronic Oral Toxicity Study - Beagle Dogs Hematologic Studies: Hematocrit, per cent Group Dietary Level (ppm) Dog Number and Sex 0 UC None 1-M 47 2-M 3-M 39 41 4-M 43 5-F 43 6-F 43 7-F 41 8-F 41 I 1 9-M 48 10-M 44 11-M 42 12-M 38 13-F 43 14-F 45 15-F 44 16-F 46 II 10 17-M 38 18-M 44 19-M 42 20-M 44 21-F 48 22-F 41 23-F 43 24-F 44 III 100 25-M 41 26-M 40 27-M 41 28-M 42 29-F 43 30-F 45 31 -F 51 32-F 46 Months: 1 3 6 12 48 50 47 56 39 44 42 56 39 42 46 54 38 38 44 51 45 40 52 56 43 42 49 52 40 42 47 52 40 41 47 50 48 51 51 53 42 42 45 46 40 43 45 48 42 44 48 49 42 43 48 48 44 47 50 55 44 46 46 55 46 46 50 56 38 40 42 46 42 43 41 47 39 44 48 48 40 43 48 50 49 47 49 56 37 38 47 53 44 41 45 - 44 46 47 54 41 47 35 . 41 35 N/A 41 44 42 42 44 45 51 50 43 45 44 43 46 49 43 45 50 50 39 44 45 46 50 51 - 57 18 50 44 43 48 48 43 47 48 47 44 43 48 45 48 48 51 43 46 48 48 52 46 se 44 43 44 45 43 40 46 48 24 47 46 46 48 47 46 46 46 49 45 44 48 40 42 44 53 44 44 47 48 52 46 - 47 47 44 42 45 45 43 - 52 ' N/A - data not available. GBRN0D2598 LEXOLDMON002878 fadtilfaoL Bi O - T S T Sue. 35 TABLE IX . TEST MATERIAL: Aroclor 1254 Two-Year Chronic Oral Toxicity Study - Beagle Dogs Hematologic Studies: Differential Leukocyte Count, percent - Neutrophils Dietary Dog Level Number Months: Group (ppm) and Sex 0 1 3 6 12 18 24 UC None 1-M 68 55 55 57 64 56 67 2-M 62 65 65 58 59 67 58 3-M 53 59 51 49 48 72 56 4-M 74 65 65 62 59 68 64 5-F 64 43 81 72 44 49 53 6-F 44 55 57 68 75 58 52 7-F 85 51 56 73 58 58 61 8-F 56 46 54 70 65 68 63 I1 II 10 in : 100 9-M 10-M 11-M 12-M 13-F 14-F 15-F 16-F 17-M 18-M 19-M 20-M 21-F 22-F 23-F 24-F 25-M 26-M 27-M 28-M 29-F 30-F 31-F 32-F 59 66 60 58 73 52 69 47 63 57 50 58 53 62 58 52 62 71 59 70 64 51 60 62 59 64 58 46 `56 61 56 55 59 70 56 44 50 46 63 70 64 71 72 78 57 67 78 65 69 47 65 67 58 61 54 58 72 55 51 49 51 51 48 65 58 66 70 58 70 59 65 59 68 71 51 54 60 58 58 50 42 63 59 63 61 69 55 71 54 54 81 57 54 54 64 84 78 - - 52 64 60 44 52 62 51 53 89 51 64 61 69 77 58 76 N/A 66 60 75 68 69 81 76 75 66 57 58 61 54 67 58 61 56 53 76 71 67 - - 58 58 62 64 58 56 75 68 60 61 71 63 57 66 65 64 61 59 68 65 60 76 67 74 61 53 - 60 N/A - data not available. GBRN002599 LEXOLDMON002879 $ndutUial BIO-TEST Sue. 36 TABLE IX continued TEST MATERIAL: Aroclor 1254 Two-Year Chronic Oral Toxicity Study - Beagle Oogs Hematologic Studies: Differential Leukocyte Count, percent - Lymphocytes Group Dietary Level (ppm) Dog Number and Sex 0 UC None ' 1-M 2-M 3-M 4-M 5-F 6-F 7-F 8-F 28 33 43 22 25 49 13 34 I 1 9-M 34 10-M 28 11-M 32 12-M 29 13-F 19 14-F 36 15-F 22 16-F 44 n - 10 17-M 29 18-M 34 19-M 38 20-M 28 21-F 39 22-F 28 23-F 39 24-F 38 m 100 25-M 32 26-M 28 27-M 35 28-M 24 29-F 30 30-F 37 31 -F 31 32-F 30 Months: 1 3 6 12 18 38 43 36 28 35 27 28 29 32 31 37 40 42 46 24 26 31 29 31 29 52 19 23 52 43 36 37 26 24 40 40 41 22 40 35 43 39 23 25 28 30 31 31 37 .36 34 36 34 32 26 39 51 43 47 34 26 31 24 24 19 38 29 22 31 23 47 31 23 35 30 44 33 26 41 45 47 44 37 39 26 30 28 27 38 26 36 29 33 27 24 44 41 30 36 38 34 47 23 26 29 36 29 39. 24 35 37 17 40 43 41 34 13 18 - - 46 29 28 47 36 33 43 42 29 37 27 22 N/A 32 30 28 13 30 38 32 30 38 32 18 27 28 40 35 29 10 44 17 37 33 18 13 24 33 40 35 44 -31 30 24 27 36 34 28 39 37 35 31 27 16 27 36 33 21 32 34 32 21 23 31 36 29 32 33 23 22 21 31 38 -- 34 N/A - data not available. GBRN002600 LEXOLDMON002880 B I O T E S T ^aioAcUauai, Sue. ' 37 TABLE IX continued TEST MATERIAL: Aroclor 1254 Two-Year Chronic Oral Toxicity Study - Beagle Oogs Hematologic Studies: Differential Leukocyte Count, percent - Monocytes Dietary Dog Level Number Months: Group (ppm) ` and Sex 0 1 3 6 12 18 24 UC None 1-M 1 2 1 2 2 2 0 2-M 2 4 2 1 6 0 1 3-M 0 1 0 3 3 1 2 4-M 2 2 0 2 2 0 2 5-r 3 0 0 0 2 12 6-F 2 2 1 2 0 0 1 7-F 0 2 1 1 0 2 0 8-F 4 7 0 2 1 02 I 1 9-M 1 2 0 3 5 2 1 10-M 2 0 2 6 2 0 2 11-M 3 0 1 1 1 01 12-M 1 0 .1 02 10 13-F 1 0 2. 0 0 10 14-F 6 0 0 6 2 12 15-F 5 0 1 0 0 00 . 16-F 1 1 1 2 1 00 H 10 17-M 0 0 0 0 2 0 0 18-M 4 1 3 2 3 1 0 19-M 5 2 0 5 0 0 6 20-M 1 0 3 2 3 2 0 21-F 0 0 0 0 2 0 0 22-F 4 3 0 2 1 2 1 23-F 0 0 0 2 - -- 24-F 4 1 0 4 0 2 0 in 100 25-M 0 5 4 1 0 0 3 26-M 1 3 . 0 0 4 0 0 27-M 3 1 N/A 1 0 4 4 . 28-M 1 0 1 4 4 0 3 29-F , 2 1 0 2 0 l 3 30-F 1 0 0 0 0 0 2 31-F 3 2 0 1 - - 32-F 6 1 0 5 0 2 1 N/A _ .* -------------- RBRN002601 LEXOLDMON002881 JndtuUiol B I O T E S T *la&oAa(&usi, Unc. 38 TABLE IX continued TEST MATERIAL: Aroclor 1254 Two-Year Chronic Oral Toxicity Study - Beagle Dogs Hematologic Studies: Differential Leukocyte Count. percent - Eosinophils Dietary Dog Level Number Months: Group (ppm) and Sex 0 1 3 6 12 18 UC None 1-M 3 5 1 5 5 7 2-M 3 4 5 2 2 2 3-M 4 3 9 6 2 3 4-M 2 6 3 7 8 3 5-F 8 5 0 5 2 7 6-F 5 7 4 4 1 2 7-F 2 7 2 4 2 5 8-F 6 4 7 5 9 4 I 1 9-M 6 9 5 8 10 '6 10-M 4 5 6 5 7 4 11-M 5 5 3 6 6 3- 12-M 12 4 4 5 2 2 13-F 7 5 2 0 4 7 14-F 6 6 4 4 5 7 15-F 4 2 8 2 4 4 16-F 8 3 4 10 10 7 H 10 ' 17-M 18-M 19-M 20-M 21-F 22-F 23-F 24-F 8 5 7 13 8 6 3 6 12 4 3 16 3 6 2 1 63 1 4 362 3 5 56 4 8 12 12 6 2 6 3 10 2122 32- . 7 7 9 10 m 100 25-M 5 0 2 4 1 5 26-M 0 4 2 4 l 5 27-M 3 1 N/A 1 6 2 28-M 5 2 2 2 2 1 29-F 4 3 5 8 5 5 30-F 11 3 4 7 9 3 31-F 6 4 2 4 - - 32-F 2 1 5 4 4 9 24 6 5 6 4 6 10 4 4 14 7 4 4 5 4 4 9 2 9 5 8 4 2 * 3 2 1 6 2 5 7 5 N/A - data not available 6BRN002602 LEXOLDMON002882 $ndM6h*al BIO-TEST Jai*tiiauc, 9*e. 39 TABLE IX continued ' TEST MATERIAL: Aroclor 1254 Two-Year Chronic Oral Toxicity Study - Beagle Dogs Hematologic Studies: Differential Leukocyte Count, percent - Basophils Dietary Dog Level Number Months: Group (ppm) and Sex 0 1 3 6 12 18 24 UC None 1-M 0 0 00 1 00 2-M 0 0 0 0 1 0 0 3-M 0 0 0 0 1 0 2 4-M 0 1 1 0 0 0 2 5-F 0 0 0 0 0 0 0 6-F 0 0 1 0 0 0 0 7-F 0 0 0 0 0 0 0 8-F 0 0 0 0 0 0 0 I1 * II 10 III- 100 - 9-M 10-M 11-M 12-M 13-F 14-F 15-F 16-F 17-M 18-M 19-M 20-M 21-F 22-F 23-.F 24-F 25-M 26-M 27-M 28-M 29-F 30-F 31-F 32-F 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 000 000 0 10 000 000 000 0 00 0 00 000 0 00 0 00 000 000 000 0 00 00 1 0 00 0 00 0 N/A 0 000 000 000 000 020 2 0 00 0 -0 00 00 01 00 22 10 01 00 00 01 01 -00 00 10 11 00 10 00 -m 11 2 0 0 0 1 2 1 0 0 5 2 0 l 0 0 2 0 1 0 0 0 0 N/A - data not available. GBRN002&03 LEXOLDMON002883 'jndtUkial BIO-TEST Jmc. 40 F. Blood Chemistry Studies The results of these determinations are presented in Tables X through XIV. Dashes in the tables indicate animals that have died during the study. No significant abnormalities were noted at any of the levels tested. GBRN00260M LEXOLDMON002884 fadtuUial BIO-TEST JoJ*clyUc4, Due. 41 TABLE X TEST MATERIAL: Arodor 1254 Two-Year Chronic Oral Toxicity Study - Beagle Bogs Blood Chemistry Studies: Blood Urea Nitrogen, mg/100 ml Dietary Dog Level Number Months: Group (ppm) and Sex 0 1 3 6 12 18 UC None 1-M 11 15 16 8 14 11 2-M 9 14 12 10 16 10 3-M 8 10 12 14 11 9 4-M 10 10 12 9 14 9 5-F 11 14 10 24 13 9 6-F 11 13 15 25 14 16 7-F 11 21 16 20 17 17 8-F 13 16 15 18 15 13 I 1 9-M 12 14 . 12 13 12 12 10-M 12 14 15 14 14 13 11-M 10 9 13 14 9 8 12-M 9 15 16 12 9 10 13-F 13 12 13 15 15 8 14-F 17 16 19 15 17 12 15-F 10 9 11 10 11 9 16-F 11 9 13 10 11 9 II III . 10 17-M 8 12 12 15 10 10 18-M 5 9 9 13 11 11 19-M 7 9 13 14 13 12 20-M 8 12 16 12 12 11 21-F 12 14 12 12 12 12 22-F 14 13 8 15 14 11 23-F 12 11 12 11 - - 24-F 15 14 19 14 16 13 100 25-M 10 12 12 12 7 9 26-M 10 10 13 17 10 10 27-M 12 16 N/A 18 15 13 28-M 9 15 13 9 8 8 29-F 16 14 14 15 15 13 30-F 15 11 13 13 12 10 31-F 11 12 15 12 - - 32-F 14 17 12 10 15 10 24 10 9 8 11 10 13 14 13 14 15 11 12 8 16 8 7 10 12 10 12 9 12 - 11 9 9 14 10 12 9 - 9 N/A - data not available. CBDMnnox LEXOLDMON002885 JtfJtuiual BIO-TEST Sne. 42 TABLE XI . TEST MATERIAL: Arodor 1254 Two-Year Chronic Oral Toxicity Study - Beagle Dogs Blood Chemistry Studies: Serum Glucose, mg/100 ml ' Dietary Dog Level Number Months: Group (ppm) and Sex 0 1 3 6 12. -18 24 UC None 1-M 83 108 106 78 107 104 103 2-M 85 101 118 103 120 106 112 3-M 111 115 106 106 111 111 103 4-M 84 104 100 109 90 104 98 5-F 98 111 68 93 101 106 114 6-F 87 104 100 85 101 98 98 7-F 74 101 94 101 94 102 100 8-F 97 117 106 112 97 108 109 I 1 9-M 94 115 112 106 101 100 100 10-M 94 108 106 97 107 106 114 11-M 91 106 102 100 111 106 105 12-M 83 106 103 100 104 104 103 13-F 106 102 105 109 101 100 100 14-F 89 106 103 100 104 94 100 15-F 106 104 106 124 104 110 ` 107 16-F 90 114 106 112 104 111 116 II 10 17-M 94 105 106 97 87 110 100 18-M 100 110 103 112 104 102 93 19-M 109 110 100 103 107 106 103 20-M 81 99 94 97 94 98 100 21-F 95 97 97 112 104 100 116 22-F 104 120 75 131 101 102 98 23-F 92 115 103 100 - - - 24- F 87 105 105 105 101 96 107 III - 100 25-M 87 107 97 118 83 no 109 26-M 60 95 100 118 94 104 116 27-M 85 106 N/A 123 104 113 109 28-M 81 106 94 109 114 100 100 29-F 96 108 100 116 97 102 103 30-F * 94 120 109 109 31 -F 82 94 88 100 97 102 107 -m - 32-F 113 94 97 105 87 100 98 N/A - data not available BBRN002606 LEXOLDMON002886 fnJutUial BIO-TEST JtA&ai&ieA, Due. 43 TABLE XH TEST MATERIAL: Arodor 1254 Two-Year Chronic Oral Toxicity Study - Beagle Dogs Blood Chemistry Studies: Serum Alkaline Phosphatase, King-Armstrong Units/100 ml Croup UC I II in Dietary Level (ppm) None 1 10 100 Dog Number and Sex 0 1-M 2-M 3-M 4-M 5-F 6-F 7-F 8-F 12. 3 17. 9 10. 1 9.9 7.0 10. 5 13.7 9. 9 9-M 10-M 11-M 12-M 13-F 14-F 15-F 16-F 7.7 8.4 6. 3 10.3 12. 5 8.2 7.0 6.7 17-M 18-M 19-M 20-M 21-F 22-F 23-F 24-F 8.6 10.3 9.9 11.6 8.2 12. 1 8.8 10.3 25-M 26-M 27-M 28-M 29-F 30-F 31-F 32-F 9.0 10.5 11.6 12. 1 7.0 6.7 10.9 9.0 1 14.5 16.1 10.7 11.6 10.7 11.0 10.2 12.4 Months: 3 6 12 18 6.6 5.2 9.2 10.3 5.8 3.5 5.4 4.2 8.0 3.0 5.4 4.9 4.6 3.3 6.2 3.3 6.2 4.9 7.7 5.6 4.4 3.8 4.4 2.5 2.3 2.0 5.2 5.6 4.4 4.4 4.4 4.4 7.8. 3.4 3.2 2.9 3.8 8.2 3.9 3.2 3.2 3. 1 7.4 3.2 3.2 2. 5 3, 3 12.6 8.0 6. 1 6.0 6. 4 16.6 12.9 4.2 9. 1 8.8 12.9 4.3 3.3 4.0 4. 1 11.8 3.9 5.2 3.6 3. 8 9.0 3.7 4.6 2.7 3.8 8.4 3.4 5.7 2.9 2.3 7.8 5.4 5.4 6.6 8. 1 15.0 6.8 6.2 7.9 6.2 12.8 6.4 5.4 5.4 5.5 12.8 4.5 3.0 3.8 3.8 8.8 12.7 3.9 5.0 4.4 11.6 11.7 3.9 - m 12.5 6.4 4.6 4.8 6.4 12.5 6.6 5.1 9.8 7.5 11.6 8.2 6.2 8.8 9.9 14. 5 N/A 6.8 9.4 13.4 10.3 7.0 6. 1 7.2 13.2 10.3 3.9 4.2 4.2 5. 1 7.8 3.6 8.9 3.6 4.4 8.0 13.4 6.8 - - 7.4 6.6 4.6 6.4 6.6 24 7.4 7.4 4.9 3. 1 2.9 6.2 4.4 5.5 3. 1 3. 6 3. 1 7.4 10.4 7.6 5.8 4.0 3. 1 8.4 7.4 8.6 3.3 11.7 9.9 10.1 13.4 12.8 9.4 6.5 4.9 10.4 N/A - data not availahl*. GBRND02607 LEXOLDMON002887 ,,/ B ( O T S T 'uo*ala'ii&, Hite. 44 TABLE Xin TEST MATERIAL: Arodor 1254 Two-Year Chronic Oral Toacity Study - Beagle Dogs Blood Chemistry Studies: Serum Glutamic-Oxalacetic Transaminase, Dade Units /ml Dietary Dog Level Number Months: Group (ppm) and Sex 0 1 3 6 12 18 24 UC None 1-M 42 31 33 23 27 27 23 2-M 40 35 . 36 28 24 22 18 3-M 31 28 27 25 23 29 18 4-M 30 24 25 25 21 26 21 5-F . 36 29 28 21 20 25 20 6-F 42 31 41 36 24 26 21 7-F 33 30 39 38 26 38 23 8-F 34 29 38 25 26 37 22 I 1 9-M 44 32 32 22 21 27 19 10-M 35 32 31 31 25 26 22 11-M 38 28 29 25 23 29 20 12-M 38 30 32 26 23 26 17 13-F 38 35 32 30 29 35 20 14-F 15-F 42 36 30 31 38 30 30 39 31 26 24 31 17 15 16-F 38 29 38 21 28 34 20 U 10 m 100 17-M 42 23 26 25 24 28 16 18-M 40 31 30 34 30 37 20 19-M 37 29 33 31 24 24 17 20-M 42 30 30 31 24 30 16 21-F 40 24 28' 32 24 37 20 22-F 41 26 33 37 25 24 18 23-F 43 25 27 31 - m- 24-F 43 31 37 32 28 34 20 25-M 32 19 20 22 32 20 14 26-M 40 23 28 23 22 26 16 27-M 23 22 N/A 37 36 37 21 28-M 40 34 44 37 36 36 20 29-F 35 26 33 36 23 30 30 30-F 33 27 32 33 26 37 31 31-F 36 32 33 32 - -- 32-F 40 28 35 25 22 30 22 N/A - data not available. F'lnn .nm.*-- - * *- GBRN00260B LEXOLDMON002888 'frduibiol BIO-TEST 2oi&alInto, Sue. 45 TABLE XIV. TEST MATERIAL: Aroclor 1254 Two-Year Chronic Oral Toxicity Study - Beagle Dogs Blood Chemistry Studies: Serum Glutamic-Pyruvic Transaminase, Dade Units/ml Group UC Dietary Level (ppm) None Dog Number and Sex 1-M 2-M 3-M 4-M 5-F 6-F 7-F 8-F 0 55 47 36 22 31 38 35 24 I 1 9-M 36 10-M 30 11-M 38 12-M 35 13-F 39 14-F 31 15-F 31 16-F 27 II 10 17-M 32 18-M 24 19-M 31 20-M 30 21-F 17 22-F 52 23-F 24-F 27 35 HI - . 100 25-M 26-M 27-M 28-M 29-F 30-F 31-F 32-F 26 26 20 33 30 29 31 41 Months: 1 3 6 12 18 43 62 43 73 76 51 50 39 51 41 39 39 49 52 34 22 24 28 34 18 31 20 31 31 21 36 39 63 41 28 36 40 57 41 35 28 31 43 36 23 38 46 28 40 `27 38 35 38 41 31 40 41 34 44 35 43 43 32 46 49 43 35 66 46 35 32 32 33 29 21 37 37 47 35 30 32 34 49 37 34 31 39 26 36 34 19 24 36 37 27 32 27 34 40 31 30 37 14 37 34 37 30 47 27 32 37 25 82 40 35 26 20 37 - - 41 39 52 38 35 20 20 19 22 25 16 31 N/A 38 19 27 25 28 36 42 20 34 44 35 31 40 30 22 37 22 24. 32 35 52 54 32 34 37 36 36 39 -- 24 23 24 60 42 39 29 30 36 39 29 38 40 37 46 31 22 28 38 33 23 34 35 30 35 41 24 35 46 24 56 54 28 GBRN00260? LEXOLDMON002889 B1OTEST 9*c. 46 G. Urine Analyses The results of these determinations are presented in Tables XV through XIX. Dashes in the tables indicate animals that died during the study. No significant abnormalities were noted at any of the levels tested. GBRN002610 LEXOLDMON002890 faiuiUiat BIO-TEST 2aiMivUl, Sue. TABLE XV TEST MATERIAL: Aroclor 1254 Two-Year Chronic Oral Toxicity Study - Beagle Cogs Urine Analyses: Albumin, mg/100 ml 47 Dieta ry Oog Level Number Months: Group (ppm) . and Sex 0 1 3 6 12 18 24 UC I II % III None 1-M 2-M 3-M 4-M 5-F 6-F 7-F 8-F 1 9-M 10-M 11-M 12-M 13-F 14-F 15 -F 16-F 10 17-M 18 -M 19-M 20 -M 21 -F 22-F 23-F 24-F 100 25 -M 26 -M 27-M 28-M 29-F , 30-F 31 -F 32-F 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1-10 0 0 0 1-10 1-10 0 0 1-10 0 0 0 1-10 0 0 00 00 0 1-10 0 0 0 0. 0 0 00 00 0 0 0 0 0 0 0 10 0 1-10 0 0 0 0 1-10 1-10 0 0 0 0 0 0 10 00 000 00 000 00 000 0 0 0 0 10 00 000 0 0 11-50 0 0 00 0 1-10 0 00 000 00 000 00 000 00 000 00 0- - 00 000 0 1-10 0 0 0 0 1-10 1-10 0 0 0 0 0 0 10 0 0 0 0 10 00 000 00 000 0 1-10 0 m m 0 0 0 0 10 0 0 0 0 0 0 0 0 0 0 0 0 0. 0 0 0 0 0 0 0 0 0 - 0 0 0 0 0 0 0 - 0 Note: BUMINTEST, Ames Company,, Inc. , Elkhart, Indiana. GBRN002611 LEXOLDMON002891 B10*TEST Sue. 48 TABLE XVI TEST MATERIAL: Aroclor 1254 Two-Year Chronic Oral Toxicity Study - Beagle Dogs Urine Analyses: Glucose, mg/100 ml Dietary Dog Level Number Months: Group (ppm) and Sex 0 1 3 6 12 18 24 UC None 1-M 0 0 0 0 0 0 0 2-M 000 0 0 00 3-M 000 00 00 4-M 000 0 0 00 5-F 0 0 0 0 0 0 0 6-F 0 0 0 0 0 0 0 7-F 0 0 0 0 0 0 0 8-F 0 0 0 0 0 0 0 I 1 9-M 000 0 0 0 0 10-M 000 0 0 00 11-M 000 0 0 .0 0 12-M 0 00 0 0 00 13-F 000 0 0 00 14-F 000 0 0 00 15-F 000 0 0 0. 0 16-F 000 0 0.0 0 II 10 17-M 000 0 0 00 18 -M 000 0 0 00 19-M 000 0 0 00 20 -M 0 0 0 0 0 00 21 -F 000 0 0 00 22-F 23-F 000 000 0 0 00 0 m -- 24-F 000 0 0 00 III 100 25-M 000 0 0 00 26 -M 0 0 0 0 0 00 27-M 000 0 0 00 28 -M 0 0 0 0 0 00 29-F 000 0 0 00 30-F 000 0 0 00 31 -F 000 0 - -- 32-F 000 0 0 00 Note: COMBISTIX, Ames Company, Inc., Elkhart. Indiana. GBRN002612 LEXOLDMON002892 9*dMikJal B I O T E S T JaienniMiti, Jlnc. 49 TABLE XVH TEST MATERIAL: Aroclor 1254 Two-Year Chronic Oral Toxicity Study - Beagle Dogs Urine Analyses; pH Dieta ry Dog Level Number Months: Group (ppm) and Sex 0 1 3 6 12 18 24 UC None 1-M 98 7 8 8 88 2-M 568 9 9 99 3-M 968 8 8 89 4-M 867 9 7 88 5-F 567 78 98 6-F 5 6 9 5 8 6 6 7-F 865 5 8 96 8-F 8 8 7 5 7 9 6 I 1 9-M 98 8 8 7 .8 6 10-M 68 9 9 9 89 11 -M 679 9 8 88 12-M 68 7 6 9 '7 8 ` 13 -F 5 9 5 6 6 76 14-F 6 6 8 6 6 86 15-F 58 7 79 78 16-F 887 9 9 79 II 10 17-M 697 9 7 68 18-M 595 9 9 87 19-M 797 76 87 20 -M 98 5 9 8 99 21 -F 7 5 7 5 8 69 22-F 9 7 N/A 8 6 76 23-F 799 5- -- 24-F 5 6 7 5 7 68 in 100 25 -M 7 7 7 9 6 8 8 * 26 -M 988 9 7 88 27-M 5 7 N/A 6 7 8 5 28-M 678 7 8 85 29-F 577 67 77 30-F 5 6 6 6 8 87 31 -F 5 69 5- -- 32-F 678 5 6 97 N/A - data not available. Note: COMBISTIX, Ames Company, Inc., Elkhart, Indiana. SBRN002613 LEXOLDMON002893 SmJuiUial B I O T E S T $* TABLE XVIII TEST MATERIAL: Arodor 1254 Two-Year Chronic Oral Toxicity Study - Beagle Dogs Urine Analyses: Specific Gravity 50 Dietary Dog Level Number Months: Group (ppm) and Sex 0 1 3 6 12 18 24 UC None 1 -M 1.054 1. 032 1.032 1. 032 1.022 1.022 1. 015 2-M 1.032 1. 019 1.024 1.043 1.041 1.045 1. 029 3-M 1.032 1. 024 1.024 1.057 1.017 1.034 1. 022 4-M 1.034 1.019 1.032 1.038 1. 030 1.036 1.032 5-F 1.026 1. 010 1.015 1.022 1.015 1.026 1.012 6-F 1.026 1.012 1.026 1.032 1.022 1.026 1. 019 7-F 1.024 1.019 1.038 1.041 1.027 1.038 1.019 8-F 1.032 1.029 1.026 1.032 1.032 1.043 1.022 I 1 9-M 1.045 1. 038 1.032 1.050 1.045. 1.048 1.038 10-M 1.045 1.032 1.024 1. 045 1.026 1.038 1. 022 11-M 1.045 1.019 1. 022 1. 043 1.017 1. 029 1. 019 12-M 1.045 1. 012 1.019 1.054 1.015 1.035 1.032 13-F 1.041 1. 012 1. 024 1. 048 1.006 1.041 1.015 14-F 1. 032 1.029 1.026 1.026 1.008 1. 048 1.015 15 -F 1.036 1.01Z 1.029 1. 028 1.020 1. 050 1.017 16-F 1.045 1. 007 1. 032 1.030 1.040 1. 042 1.015 II ' 10 17 -M 18-M 19-M 20-M 21 -F 22-F 23-F. 24-F in 100 25 -M 26-M 27-M 28 -M 29-F 30-F 31 -F 32-F 1.024 1.029 1. 032 1.032 1.026 1. 015 1.032 1. 041 1.019 1.012 1.015 1. 032 1. 052 1.032 1. 022 1. 022 1.026 1. 026 1. 034 1.050 1. 034 N/A 1.041 1. 048 1.045 1.043 1. 048 1.041 1.034 1.033 1.038 1. 041 1.033 1.021 1.040 1.029 1.040 1.030 . 1.033 1.015 1.010 1. 010 1.041 1.034 1.041 m 1.045 1. 029 1.017 1.032 1. 026 1.010 1.010 - 1.015 1.026 1.015 1.032 1.015 1.045 1.038 1.045 1.041 1.032 1.026 1.029 1.007 1.012 1.022 1.036 1.024 1.041 1.043 N/A 1.010 1.045 1.045 1. 050 1.041 1.050 1.061 1.054 1.041 1.017 1.029 1.038 1.041 1. 043 1.050 1.012 1.003 1.026 1.029 1.006 1.041 1.010 1.050 1.043 1.034 1.036 1.038 1.026 1.041 1.045 1.026 1.024 1.026 1.026 N/A - data not available. GBRN002614 LEXOLDMON002894 B I O T E S T JaJ&tafaiai, Sue. . TABLE XIX 51 TEST MATERIAL: Aroclor 1254 Two-Year Chronic Oral Toxicity Study - Beagle Cogs Urine Analyses: Microscopic Elements, average per high power field - Leukocytes Dietary Dog * Level Number Months: Group (ppm) and Sex 0 1 3 6 12 18 24 UC None 1-M 2-M 3-M 4-M 5-F 6-F 7-F 8-F 000 000 000 000 000 000 000 000 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 00 1 1 9-M 000 0 0 .0 0 10-M 0 0 0 0 0 0 0 11 -M 0 0 0 0 0 0 0 12-M 0 0 20 0 0 'o 0 13-F 000000 0 14-F 0 0 10 . 0 0 0 0 15-F 000 000 0 16-F 000 0 00 0 II ' 10 17-M 0. 0 0 0 0 0 18 -M 000 000 19-M 0 0 0 0 0 0 20 -M 0 0 0 0 0 0 21 -F 000 000 22-F 0 8 N/A 0 0 0 23-F 0 10 0 8 mt - 24-F 0 0 0 0 0 0 0 0 0 0 0 0 - 0 in 100 25-M 0 0 0 0 0 0 0 * 26 -M 0 0 0 0 0 0 0 27-M 0 0 N/A 0 0 0 0 28 -M 0 0 0 0 0 0 0 29-F 0 0 0 0 25 0 0 30-F 0 0 0 0 0 0 0 31-F 0 0 0 0 - - 32-F 0 0 0 15 0 0 0 . N/A - data not available GBRN002615 LEXOLDMON002895 fajitihial BIO-TEST ^a&o'vtL*\ioA, Sue. TABLE XIX- continued - 52 TEST MATERIAL: Aroclor 1254 Two-Year Chronic Oral Toxicity Study - Beagle Dogs Urine Analyses: Microscopic Elements, average per high power field - Erythrocytes Dietary Dog Level Number Group (ppm) and Sex 0 UC None 1-M 2-M 3-M 4-M 5-F 6-F 7-F 8-F 0 0 0 0 0 0 0 0 I 1 9-M 0 10-M 0 11-M 0 12-M 0 13-F 0 14-F 0 15-F 0 16-F 0 II 10 17-M 0 18 -M 0 19-M 0 20 -M 0 21 -F 0 22-F 0 - 23-F 0 24-F 0 Ill 100 25-M 0 26 -M 0 27-M 0 28 -M 0 29-F 0 30-F ' 0 31-F 0 32-F 0 Months: 1 3 6 12 18 00 00 0 00 000 00 000 00 000 00 000 00 000 00 000 00 000 0. 0 00 00 00 0 25 00 0 25 00 0 0 0 0 0 .0 0 0 0 0 00 0 .0 00 00 00 00 00 00 000 00 000 00 000 00 000 00 000 0 N/A 0 0 0 00 0m - 00 000 00 000 00 000 0 N/A 0 0 0 00 000 00 000 00 000 0 50 0mm 00 000 24 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 N/A - data not available. 6BRN0026 1 6 LEXOLDMON002896 B 1 O T E S T jjaivutitHiM, }tte. TABLE XIX continued . 53 TEST MATERIAL: Aroclor 1254 Two-Year Chronic O.ral Toxicity Study - Beagle Dogs Urine Analyses: Microscopic Elements, average per high power field - Crystals Dietary Dog Level Number Group (ppm) and Sex 0 UC None 1-M 1-3 2-M 1-3 3-M 1-3 4-M 1-3 5-F 1-3 6-F 1-3 7-F 1-3 8-F 4 Months: 1 3 6 12 18 1-3 1-3 1-3 1-3 1-3 1-3 1-3 1-3 1-3 1-3 1-3 1-3 1-3 1-3 1-3 1-3 1-3 1-3 1-3 1-3 1-3 1-3 1-3 1-3 1-3 1-3 1-3 1-3 1-3 1-3 1-3 1-3 1-3 1-3 1-3 1-3 1-3 1-3 1-3 1-3 24 1-3 1-3 1-3 1-3 1-3 1-3 1-3 1-3 I 1 9-M 1-3 1-3 1-3 1-3 1-3 . 1-3 1-3 10-M 1-3 1-3 1-3 1-3 1-3 1-3 1-3 11-M 1-3 1-3 1-3 1-3 1-3 1-3 1-3 12-M 1-3 1-3 1-3 1-3 1-3 1-3 1-3 13 -F 1-3 1-3 1-3 1-3 1-3 1-3 1-3 14-F 1-3 1-3 1-3 1-3 1-3 1-3 1-3 15-F 1-3 1-3 1-3 1-3 1-3 1-3 1-3 16-F 1-3 1-3 1-3 1-3 1-3 1-3 1-3 II in * 10 17-M 18 -M 19-M 20-M 21 -F 22-F 23-F 24-F 100 25 -M 26 -M 27-M 28-M 29-F 30-F 31 -F 32-F 1-3 1-3 5-8 1-3 1-3 1-3 1-3 1-3 1-3 1-3 1-3 1-3 1-3 1-3 1-3 1-3 1-3 1-3 1-3 1-3 1-3 1-3 1-3 1-3 1-3 1-3 1-3 1-3 1-3 1-3 1-3 1-3 1-3 1-3 1-3 1-3 1-3 1-3 1-3 1-3 1-3 1-3 N/A 1-3 1-3 1-3 1-3 1-3 1-3 - - 1-3 1-3 1-3 1-3 1-3 1-3 1-3 1-3 1-3 1-3 1-3 1-3 1-3 1-3 1-3 1-3 N/A 1-3 1-3 1-3 1-3 1-3 1-3 1-3 1-3 1-3 1-3 1-3 1-3 1-3 1-3 1-3 1-3 1-3 1-3 1-3 1-3 1-3 - - 1-3 1-3 1-3 1-3 1-3 1-3 1-3 1-3 1-3 1-3 1-3 1-3 1-3 1-3 1-3 1-3 1-3 1-3 1-3 N/A - data not available GBRN002617 LEXOLDMON002897 BIO-T ES T JaieaaL,vUai, Hue. 54 H. Pathologic Findings 1. Organ Weight Data Organ weight data are presented in Tables XX through XXVIII. Four animals show liver to body weight ratios higher than untreated controls. In animals 23-F and 31-F, these values are the result of poor overall body weight gains rather than actual organ weight elevations. Dog 26-M shows a ratio at the upper limit of the range considered normal for the beagle. The value for dog 27-M represents a elevation in liver to body weight ratio. A male (no. 17) receiving 10 ppm Aroclor 1254 and three males (nos. 25,26,23) receiving 100 ppm showed depressed absolute testes weights and testes to body weight ratios. The lack of proper size is con sidered an atrophic condition resulting from a ehronic testicular inflamma tion found in these four animals. This is discussed further under 2. Gross and Histologic Findings. All other organ to body weight ratios fall within normal limits. 6BRN0026IS LEXOLDMON002898 BIO-TEST Ja&o*aI&tiU, Jmc. 55 TABLE XX TEST MATERIAL: Aroclor 1254 Two-Year Chronic Oral Toxicity - Beagle Dogs Organ Weight Data: Liver Group UC Dietary Level (ppm) None Dog Number and Sex 1-M 2-M 3-M1 4-M2 5-F, 6-F 1 7-F 8-F 2 Organ Weight <g) 269. 3 397.2 346. 3 481.8 316.8 443.6 326. 1 355.3 Organ/Body Weight Ratio (k/I.OOOb) 35.4 39.7 34. 3 32.6 34.4 41.8 34.3 28.6 I 1 9-M 468.2 10-M 488. 1 U-M1 461.5 12-M2 531.8 13-F 2 470.2 14-F 1 478.2 15-F 428.2 16-F 365.0 35. 5 39.4 34.7 . 34. 1 34. 8 41. 2 32.7 29.0 n 10 m 100 . 17-M* 18-M2 19-M 20-M 21-F 1 22-F 23-F4 24-F 25-M1 26-M 27-M 28-M2 29-F 30-F 31-F3 32-F 2 409.8 483.5 46 2. 2 444.6 285. 8 286. 8 277. 5 317. 5 318.7 359.2 447.4 293.0 310.3 415.4 439.2 449.6 33.0 34. 8 39.5 40. 8 32. 1 36. 8 47. 8 37.8 36.6 44.9 54.6 31.8 40.8 41.5 73.2 35.7 1 Sacrificed alter 30-day recovery period. 2Sacrificed after 60-day recovery period. 3Dicd after 33 weeks of testing. rtf Irstin^i GBRN002&1? LEXOLDMON002899 BIO-TEST Jaio*a(niai, 3*0. TABLE XXI TEST MATERIAL: Aroclor 1254 Two-Year Chronic Oral Toxicity - Beagle Dogs Croup UC Organ Weight Data: Kidneys Dietary Level (ppm) Dog Number and Sex Organ Weight (K) Organ/Body Weight Ratio (b/I.OOOz) None 1-M 2-M 3-M1 4-M2 5-F 6-F 1 7-F 8-F 2 55.4 79. 2 70. 8 71.4 49.9 58.4 45.3 53.0 7.29 7.92 7.01 4.82 5.42 5.51 4.47 4. 27 I1 H 10 m 100 9-M 10-M 11-M1 12-M2 13-F 2 14-F 1 15-F 16-F 17-Ml 18-M2 19-M 20-M 21-F 1 . 22-F 23-F* 24-F 25-M1 26-M 27-M 28 -M2 29-F 30-F 31-F3 32-F2 79.5 73.0 88.5 90. 5 95.0 52. 8 74.5 63.8 64. 1 60.8 81.0 58.7 51.2 42.9 49. 3 44.0 62. 5 63.5 45.8 73.5 41.3 53.3 55.8 57.8 6.02* 5.89 6.65 ,, 5.80 7.04 4.55 5.69 5.06 5. 17 4.37 6.92 5.38 5.75 5.50 8.50 5.24 7.18 7.94 5.85 7.99 5.43 5.33 9.30 4.59 . 1 Sacrificed after 30-day recovery period. 2c*,-ririeed after 60-day recovery period. GBRNQ02620 LEXOLDMON002900 ftBIO-TEST Shc. 57 TABLE XXH TEST MATERIAL: Aroclor 1254 Two-Year Chronic Oral Toxicity - Beagle Dogs Organ Weight Data: Heart Group UC Dietary Level (ppm) None Dog Number and Sex 1-M 2-M 3-M1 4-M2 5-F 6-F 1 7-F 8-F2 Organ Weight (g) 73.6 107. 0 80. 9 128.6 70.2 93.8 89.0 113.5 Organ/Body Weight Ratio (g/l,OOOg) 9.68 10. 7 8.01 8. 69 7.63 8.85 9. 37 9. 15 I 1 9-M 105.4 1Q-M 122.3 11-Mi 106.4 12-M2 120.7 13-F 2 95.0 14-F 1 94.4 15-F 102.5 16-F 95.6 7. 9*8 9. 86 8. 007. 74 7. 04 8. 14 7. 82 7. 59 H 10 17-Mi 103.9 18-M2 121.7 19-M 20-M 21-F i 91.3 126. 1 78.4 22-F 23-F4. . 24-F 71.7 58.5 74.4 8.38 8. 76 7. 80 11. 56 8.81 9. 19 10.1 8. 86 HI 100 25-M* 68.4 26-M 79.5 27-M 45. 8 28-M2 84.9 29-F 41.3 . 30-F 87.5 * 31-F3 55.5 32-F 2 104.4 7. 86 9. 94 8.91 9.23 8.25 8. 75 9.25 8. 28 ^Sacrificed afler 30-day recovery period. ^Sacrificed after 60-day recovery period. 3fNi a#) e/Ue 11 /\f fACfl'nff. GBRN00262 LEXOLDMON002901 58 TABLE XXHI TEST MATERIAL: Aroclor 1254 Two-Year Chronic Oral Toxicity - Beagle Dogs Organ Weight Data: Brain Group UC Dietary Level (ppm) None - Dog Number and Sex 1-M 2-M 3-M1 4-M2 5-F 6-F 1 7-F 8-F 2 Organ Weight (g) 72.8 76.1 82.0 82.9 72.3 77.4 79.4 80.4 Organ/Body Weight Ratio (g/l.OOOe) 9.58 7.61 8.12 5.60 7.86 7.30 8.36 6.48 I 1 9-M 85.0 10-M 86.2 11-M1 12-M2 67.2 73.6 13-F2 75.0 14-F 1 15-F 16-F 79.1 76.2 73.2 6.44 6.95 5.05 . 4.72 5.56 .6. 82 5.82 5.81 n ' 10 m 100 17-Ml 18-M2 19-M 20-M 21-Fl 22-F 23-F* 24-F 25-M1 26-M 27-M 28-M2 29-F 30-F 31-F 3 32-F2 75.9 78.9 87.3 79.8 72.5 79.1 81.4 72.7 79.3 75.9 79. S 79.3 65.0 74.2 70.5 77.6 6.12 5.68 7.46 7.32 8.14 10.14 14.03 8.65 9.11 9.49 9.70 8.62 8.55 7.42 11.75 6. ,16 ^Sacrificed alter 30-day recovery period. ^Sacrificed after 60-day recovery period. 3 Died aflcr 33 weeks of testing. .1 . 1____ ___i... BBRN002622 LEXOLDMON002902 59 TABLE XXIV . TEST MATERIAL: Aroclor 1254 Two-Year Chronic Oral Toxicity - Beagle Dogs Organ Weight Data: Spleen Group . Dietary Level (ppm) Dog Number and Sex Organ Weight (e) Organ/Body Weight Ratio (i/1.000e) UC None 1-M 21.3 2-M 27.2 3-M1 16.3 4-M2 29.1 5-F 19.2 6-F 1 22.9 7-F 20.2 8-F 2 30. 1 2.80 2.72 1.61 1.97 2.87 2.16 2.13 2.43 I' 1 9-M 10-M 11-M 1 12-M2 13-F 1 14-F 15-F 16-F 34.7 23.3 24.9 37.2 15.2 22.8 27.0 29.3 2.63' 1.88 1.87 2.38 1. 13 1.96 2.06 2.32 n' 10 17-Ml 18-M2 19-M 20-M 21-F 1 22-F 23-F4 24-F 29.7 36.1 26.0 24.8 23.5 14.9 13.3 24.3 2.39 2.60 2.22 2.28 2.64 1.91 2.29 2.89 nr 100 25-M 1 19.9 26-M 21.8 27-M 17.2 28-M2 29.1 - 29-F 16.0 30-F 26.0 31-F 3 25.8 32-F 2 40.8 2.29 2.72 2.10 3.16 2.10 2.60 4.30 3.24 ^Sacrificed'after 30-day recovery period ^Sacrificed after 60-day recovery period. 3Died after 33 weeks of testing. GBRND02623 LEXOLDMON002903 O ( \J * I C J I ZOMAMiM, JttC. 60 TABLE XXV TEST MATERIAL: Aroclor 1254 Two-Year Chronic Oral Toxicity - Beagle Dogs Group UC Organ Weight Data: Gonads _ Dietary Level (ppm) _ Dog Number and Sex Organ Weight (*> Organ/Body Weight Ratio (e/1.000e) None 1-M 2-M 3-Ml 4-M2 5-F 6-F u 7-F 8-F 2 16.0 15.2 14. 3 20.5 0.769 2.498 1.372 0.993 2. 10 1.52 1.41 1.38 0.0836 0.236 0. 144 0.0801 I 1 9-M 17.4 10-M 15.5 11-M1 26.2 12-M2 30.5 13-F2 1.413 14-F1 1.225 15-F 1.681 16-F 0.912 1.32 1.25 1.97 . 1.96 0.105 0.106 0.128 . 0.0724 n' 10 ni 100 17-M1 18-M2 19-M 20-M 21-F 1 22-F 23-F 4 24-F 25-M1 26-M 27-M 28-M2 29-F 30-F 31-F 3 32-F 2 8.6 20.8 24.8 19.5 0.711 0.777 0.497 0.908 7.8 3.8 19.4 6.9 0. 806 1.294 0.635 1.692 0.694 1.50 2.12 1.79 0.0799 0.0966 0.0857 0.108 0.896 0.475 2.36 0.746 0.106 0.129 0.106 0.134 ISacrificcd after 30-day recovery period. ^Sacrificed after 60-day recovery period. 3Dicd after 33 weeks of testing. 4rn.i ----- * Animal in ~estrus. BBRN00262^ LEXOLDMON002904 frtiI-***3*1 fl 1 O - I ft S I JMaHitnMeA, ta 61 TABLE XXVI TEST MATERIAL: Aroclor 1254 Two-Yeir Chronic Oral Toxicity - Beagle Dogs Group UC Organ Weight Data: Adrenal Glands ; Dietary * Level (ppm) Dog Number and Sex Organ Weight (g) Organ/Body Weight Ratio (sr/l.OOOe) None 1-M 2-M 3-M1 4-M2 5-F 6-F 1 7-F 8-F2 1.170 1.480 1.350 1.132 1.569 1.834 1.308 1.297 0. 154 0.148 0.134 0.0765 0.170 0. 173 0. 138 0.105 I 1 9-M 1.850 10-M 1. 855 11-M1 1.563 12-M2 1.905 13-F2 2.525 14-F1 1.645 15-F 2.006 16-F 1.737 n 10 17-M1 1.442 18-M2 1. 879 19-M 1.427 20-M 1.677 21-F1 1.249 22-F 1.156 23-F4 1.688 24-F 1.642 0.140 0.150 0.118 0.122 0.187 0. 142 0. 153 0. 138 0. 116 0.135 0. 122 0. 153 0.140 0.148 0.291 0.195 in 100 25-M1 1.178 26-M 1.217 27-M 1.364 28-M? 1.321 29-F 1.680 30-F 1.566 31-F3 0.912 32-F2 1.618 0. 135 0.152 0.166 0.144 0.221 0. 157 0.152 0.128 iSacrificcd after 30-day recovery period. ^Sacrificed after 60-day recovery period. 3 rv - j 1------ 1. - -` *---- GBRN002625 LEXOLDMON002905 jBIU* I b) I `J*e. 62 TABLE XXVH TEST MATERIAL: Arodor 1254 . Two-Year Chronic Oral Toxicity - Beagle Dogs Organ Weight Data: Thyroid Gland Group . Dietary Level (ppm) Dog Number and Sex Organ Weight (fit) Organ/Body Weight Ratio (e/1,000c) UC None . 1-M 2-M 3-Ml 4-M2 5-F 6-F 1 7-F 8-F2 0.435 1.013 1.066 1. 101 0.615 1.692 0.631 0.952 0.0572 0. 101 0. 106 0. 0744 0. 0668 0. 160 0.0664 0.0768 I 1 9-M 1.284 10-M 1.223 11-Ml 1.096 12-M2 2.041 13-F 2 1. 167 14-F 1 1. 179 1S-F 2. 173 16-F 1.318 II 10 17-Ml 1. 334 18-M2 1.290 19-M 1.648 20-M 1. 130 21-Fl 0.606 22-F 0. 836 23-F* 0. 704 24-F 1. 186 0.0972 0.0986 0.0824. 0. 131 0.0864 0. 102 0. 166 0. 105 0. 108 0.0928 0. 141 0. 104 0.0681 0. 107 0. 121 0. 141 III 100 25-M1 0. 855 26-M 1.169 27-M 0.995 28-M2 0. 810 29-F 1.026 .30-F 1.764 ' 31-F 3 0. 826 32-F2 0.916 0.0983 0. 146 0. 121 0.0880 0. 135 0. 176 0. 138 0.0727 ^Sacrificed after 30-day recovery period. ^Sacrificed after 60-day recovery period. ^Died after 33 weeks of testine. GBRN0Q2626 LEXOLDMON002906 B I O I 6 S t ZaboAotHimi, y*e. 63 TABLE XXVin . TEST MATERIAL: Aroclor 1254 Two-Year Chronic Oral Toxicity - Beagle Dogs Organ Weight Data: Pituitary Gland Group UC . Dietary ' Level (ppm) None I1 Dog Number and Sex 1-M 2-M 3-Ml 4-M*2 * 5-F 6-F 1 7-F 8-F2 9-M 10-M 11-M1 12-M2 13-F2 14-F 1 15-F 16-F Organ Weight (*) 0.086 0. 116 0.080 0.099 0.101 0.184 0.101 0.097 0. 109 0. 155 0.110 0.095 0. no 0. 120 0.191 0.083 Organ/Body Weight Ratio (g/l.OOOe) 0.0113 0.0116 0.00792 0.00669 0.0110 0.0174 0.0106 0.00782 0. 00826 0.0125 0.00827 0.00609 0.00815 0.0103 0.0146 0.00659 n 10 17-M1 18-M2 19-M 20-M 21-F1 22-F . 23-F4 24-F 0.073 0.102 0. 092 0. 164 0.080 0.157 0.065 0.173 0.00589 0.00700 0.00786 0.0150 0.00899 0.0201 0.0112 0.0206 ni 100 25-M* 0.087 26-M 0.185 27-M 0.065 28-M2 0.073 29-F . 0.070 30-F 0.093 31-F 2 0.105 32-F 2 0.113 0.0100 0.0231 0.00793 0.00793 0.00921 0.00930 0.0175 0.00897 ^Sacrificed after 30-day recovery period. ^Sacrificed after 60-day recovery period. 2Diod after 33 weeks of testing. 4Died after 37 weeks of tcstinc. GBRN002627 LEXOLDMON002907 fajudbiat B I O * T E S T Jtaio^aianisi, Png. - 64 2. Cross and Histologic Findings Gross and histologic findings are presented in Tables XXIX through XXXII. All tissues and organs not mentioned were normal. The grading system used is as follows: + s minimal/trace ++ r mild +++ = moderate ++++ = severe 6BRN00262B LEXOLDMON002908 ft I O T E S T 2aU*atn\i*i, Sue. 65 IBT No. C7299 March 11, 1970 I have examined the tissues from one dog (23-F) from IBT No. C7299. There was chronic peritonitis evident on the serous surfaces of the stomach, intestine and bladder. This lesion was chronic and had been present for at least a week and probably longer. There was severe autolysis of the tissues. Ward R. Richter, D.V.M., M.S. Diplomate, American College of Veterinary Pathologists GBRN00262? LEXOLDMON002909 'J\td*ukial BIO-TEST Jaio*a{\i*i, Due. 66 IBT No. C7299 October 27, 1971 None of the changes observed in sections from any of the dogs in this study reveal a definite drug-related pattern. The mild to moderate non-specific chronic inflammatory changes in lung, liver, prostate gland, kidney, salivary gland, stomach, small intestine and lymph node are con sidered incidental findings, observed frequently in clinically normal, untreated dogs. The lymphoid infiltration (inflammation) seen in the thyroid of one dog (1 ppm Arodor 1254) is noted in certain blood lines (canine) and is a naturally-occurring phenomenon. The changes in testes are difficult to assess. The etiology-of the changes cannot be precisely determined, but it is thought than an_un specified infectious condition produced the inflammatory lesions observed. Donald H.' Yo^D.V.M. Diplomate, American College of Veterinary Pathologists 6BRN00263D LEXOLDMON002910 7* 1 S 3 1 * 0 1 8 T X W V C Dog Number and Sex 1 -M 2-M 3-M1 4-M2 5-F 6-F* 7-F 8-F2 TABLE XXIX TEST MATERIAL: Aroclor 1254 Two-Year Chronic Oral Toxicity Stud/ - Beagle Doge Gross and Histologic Findings Untreated Control Group Organ . - Prostate gland Lungs Lymph node (cervical) - - - - Gross _ - - - - Grade Histologic Grade - -- - Focal non-specific chronic inflammation +++ - - Focal hemorrhage (agonal) ++ Congestion + - -- - -- - -- - -r - LEXOLDMON002911 ^Sacrificed after a 30-day recovery period. ^Sacrificed after a 60-day recovery period. CD 00 7S zoa N O' w o-J .i BIO -TEST Jt LEXOLDMON002912 Dog Number and Sex 9~M 10-M 11-M? 12-M2 13-F2 14-F1 15-F 16-F TABLE XXX TEST MATERIAL: Aroclor 1254 Two-Year Chronic Oral Toxicity Study - Beagle Doge Gross and Histologic Findings , Test Group I: 1 ppm Orean Stomach Stomach - Stomach Thyroid gland Uterus Uterus Gross Numerous pinpoint nodules (pyloric region) Numerous pinpoint nodules (pyloric and cardiac regions) Grade + +++ * - Numerous pinpoint nodules (pyloric region) - + . In estrous In estrous Histologic ' Grade -. - - Chronic interstitial inflammation - 1 ++++ -* GCO) * Sacrificed after a 30-day recovery period. Xza ^Sacrificed after a 60-day recovery period. a No* (A N o> 00 BIO -TEST LEXOLDMON002913 Dog Number and Sex 17-M1 Orsan Testes 18-M - 19-M . Lungs 20-M Lungs 21-F1 Lungs 22-F C) u31 z o N& WW -' TABLE XXXI TEST MATERIAL: Aroclor 1254 Two-Year Chronic Oral Toxicity Study - Beagle Dogs Gross and Histologic Findings Test Group II: 10 ppm Gross Undersize, appear underdeveloped .- K Grade +++ - Histologic Grade t Chronic focal inflammation Diffuse testicular degeneration Diffuse hyperplasia of the interstitial cells Aspermatogene si s -. Focal non-specific chronic inflammation Focal non-specific chronic inflammation Focal non-specific chronic inflammation Focal hemorrhage + ++ + -` ++ ++ + - N(hO Dog Number and Sex 4 23-F TABLE XXXI continued TEST MATERIAL: Aroclor 1254 Two-Year Chronic Oral Toxicity Study - Beagle Doge Gross and Histologic Findings Test Group II: 10 ppm . OrganGrossGrade . HistologicGrade (examination 8-12 hours postmortem) Gall bladder Distended twice normal size with +++ bile Liver -- Peritoneum Thickened, opaque and constricted +++ about gastrointestinal tract and urinary bladder; no lesions or blockages of gastrointestinal tract, in spite of intestinal serosae adhering to one another and causing pressure and torsion; abdominal cavity contained approximately 100 ml translucent fluid. Autolysis of all tissues ++++ Congestion 4- Chronic peritonitis on . +++ serosal surfaces of intestine, stomach and urinary bladder; no acute lesions present. is f. ii o> O m i/i r LEXOLDMON002914 CD a3zo0 -i o oW o04 c jdtubuaL B IO -TE S T LEXOLDMON002915 Dog Number and Sex 23-F4 24-F TABLE XXXI continued TEST MATERIAL: Aroclor 1254 Two-Year Chronic Oral Toxicity Study - Beagle Dogs Gross and Histologic Findings Test Group II: 10 ppm Organ Lymph node (mesenteric) Lungs Stomach Gross Tissues appear to have dissolved, no cellular tissue suitable for fixation. . - Numerous pinpoint nodules (pyloric region) Grade - Histologic - Grade - Focal non-specific chronic ++ inflammation ++ 1 Sacrificed after a 30-day recovery period. `Sacrificed after a 60-day recovery period. *Died after 37 weeks on test. t G) CG 3D oO2 0IN) Uw J - - 1 S3 1 " OIB jwwpty Dog * Number and Sex 25-M1 Oman Colon Ileum Abdomen Stomach Testes TABLE XXXII TEST MATERIAL: Aroclor 1254 Two-Year Chronic Oral Toxicity Study - Beagle Doga Gross and Histologic Findings Test Group III: 100 ppm Gross ' Petechiae Ileocolic valve tissues swollen and reddened, causing partial blockage of gastrointestinal ' tract. Approximately 20 ml translucent fluid in abdominal cavity Numerous pinpoint nodules (pyloric and cardiac regions) Undersize, appear underdeveloped Grade +++ +++ Histologic Grade ++++ +++ Chronic diffuse inflammation +H*++ Focal testicular degeneration +++ Aspermatogenesis. LEXOLDMON002916 G) DO 7} oZ -N1 oN O 0* LEXOLDMON002917 Dog Number and Sex 25-M* 26-M Orsan Prostate gland Salivary gland Liver Lungs Testes cn awj oz o IoS) w TABLE XXXII continued TEST MATERIAL: Aroclor 1254 Two-Year Chronic Oral Toxicity Study - Beaglo Doga Gross and Histologic Findings Test Group III: 100 ppm .V Gross - -. - Undersize, appear underdeveloped Grade Histologic Grade Focal non-specific chronic ++ inflammation - Focal non-specific chronic + inflammation - Focal non-specific chronic ++ inflammation - . Focal non-specific chronic + inflammation +++ Chronic focal inflammation + Diffuse testicular degenera- ++ tion Diffuse hyperplasia of the + interstitial cells Aspermatogenesis - -J 1 s 31* o I a T^-r^F BIO -TEST LEXOLDMON002918 Dog Number and Sex 27-M Organ Lungs Stomach 2 28-M Kidneys Stomach Testes t Q00 21 2ao N0> W 00 TABLE XXXII continued TEST MATERIAL: Aroclor 1254 Two-Year Chronic Oral Toxicity Study - Beagle Doga Groaa and Histologic Findings Test Group III: 100 ppm Gross - Numerous pinpoint nodules (pyloric and cardiac regions) Numerous scars and identations on external cortical surfaces Numerous pinpoint nodules (pyloric and cardiac regions) Undersize, appear underdeveloped % Grade Histologic Grade - Focal non-specific chronic ++ inflammation +++ +++ +++ * +++ Chronic diffuse inflammation ++ Diffuse testicular degenera +++ tion Diffuse hyperplasia of the + interstitial cells Diffuse aspermatogenesis --I vJtufaisL B IO -T E S T LEXOLDMON002919 Dog Number and Sex 29-F Oman Lungs Stomach 30-F Kidneys Stomach TABLE XXXII continued TEST MATERIAL: Aroclor 1254 Two-Year Chronic Oral Toxicity Study - Beagle Doge Groea and Histologic Findings Test Group HI: 100 ppm Gross - Numerous pinpoint nodules (pyloric and cardiac regions) * Numerous pinpoint nodules (pyloric region) Grade - 444 Histologic Grade Focal non-specific chronic ++ inflammation * Focal non-specific chronic 4+ inflammation (medulla). ++ i CD to X O2 m-4 a No W <0 i IS 31*018 JW T # TABLE XXXII continued TEST MATERIAL: Aroclor 1254 Two-Year Chronic Oral Toxicity Study - Beagle Dogs Gross and Histologic Findings Test Group III: 100 ppm Dog Number and Sex ______ Oraan____________ Gross Grade Histoloeic Grade 31-F3 32-F2 (animal died owing to traumatic injuries sustained in fight; examination performed immediately postmortem) Liver Lymph node _- - Hyperemia ' - Focal granulomatous + + (mesenteric) inflammation . Hyperemia ++ Pituitary gland Small intestine - - - Hyperemia - Focal inflammation + + (duodenum) Stomach - - Focal inflammation Lungs - - Focal interstitial pneumonia + ^Sacrificed after a 30-day recovery period. mBJ ZSacrificed after a 60-day recovery period. ^Died after 33 weeks on test. 3z) a aM or* a O--ki LEXOLDMON002920