Document 6B1yrmNDqXYmg6rZXjq5pYg99

P M&&6 - 32 f 451 Determination of Ultraviolet DuPont report # 12734 t Test Guideline(s) Authors Maiy A. Kaiser, Ph.D. (study director) Raymond E. Richardson Robert C Buck, Ph. D. Study Completion Date 16 July 2002 Report Date 21 March 2003 Test Facility DuPont Corporate Center for Analytical Sciences Experimental Station 402/5321 and E328/162B Wilmington, DE 19880 Sponsor Telomer Research Panel c/o Rand Corporation lTelomer Research Program Company Sanitized. Does not contain T S C CBI 1 Determination of Ultraviolet Spectrum: 2 Table of Contents Title Page...................................................... Table of C ontents........................................ 1.0 Summary............................................. 2.0 General Study Inform ation.............. 3.0 Materials and M ethods..................... 3.1 Test Guidelines.......................... . 3.2 Chemical System....................... . 3.3 Test Vehicle................................. 3.4 Parameters Observed............... 3.5 Result Analysis.......................... . 3.6 Validity Criteria of the Study.... 3.7 Protocol Deviations................... . 4.0 Results and D iscussion..................... 5.0 Conclusions....................................... 6.0 Retention o f R ecords........................ 7.0 Disposal o f Test Item......................... 8.0 R eferences.......................................... Figure Figure 1 UV-VIS spectrum....................... Appendix Appendix A Chemical Analysis Summay .1 .2 3 ,3 .4 ,4 ,5 ,6 6 6 6 6 1 .7 7 7 .7 2Telomer Research Program Company Sanitized. Doss no! contain TSCA 2 Determination of Ultraviolet Spectrum a ultraviolet spectrum at 25C. was used to determine its Findings: There is no measurable absorption from 225 to 400 nm. Conclusions: There is no measurable UV absorption for this compound. 2.0 General Study Information Study Objectives The objective o f this study was to determine the ultraviolet absorption spectrum B a t 25C. Test System Justification The test system was selected by the sponsor. Study Personnel Management: Study Director: Principle Investigator: Technical Personnel: Marianne Marsi, Ph.D. Mary A. Kaiser, Ph.D. Raymond E. Richardson Raymond E. Richardson Study Execution Dates Study Initiation Date: Experimental Start Date: Experimental Completion Date Study Completion Date: 3Telomer Research Program 15-Jun-2001 15-Jun-2001 28-June 2002 12-July 2002 Company Sanitized. Does not contain TSCACB1 3. Determination of Ultraviolet Spectrum 3.0 M a t e r ia l s a n d M e t h o d s 3.1 Test Guidelines OECD Guidline 101 ws used in this study. The ultraviolet absorption spectrum of a solution is a function of the concentration, Cj, expressed in mol/L, of all absorbing species present; the path length, d, of the spectrophotometer cell, expresed in cm; and the molar absorptivity (extinction coefficient), \, of each species. The absorbance (optical density) A of the solution is then given by A = d Sj i, Ci 3.2.1 Chemical System 3.2.1.1 Test Substance The test substance was obtained from Clariant Gmbh (Germany) and was shown to bej pure via gas chromatography. ^ B H U B U B B ^ K i m p urities were detected. The major i m p u r i t y ^ ^ ^ P H ^ v a s tentatively id e n tifie d a s^ ^ H H H IH K > a se d on the mass spectral fragmentation pattern Name: Synonym: Active substance(s) CAS Name: CAS Number(s): Structure: 4Telomer Research Program 4 Determination of Ultraviolet Spectrum: 8-2 Telomer B Alcohol H Number Lot Number: EMSE Sample Number: Concentration, nominal: Concentration, analyzed: Certificate of Analysis Date: Date Received: Solubility at 25C.: Vapor pressure: Stability: Appearance/Color: Storage Conditions: H-24691 mE93386-63 03-Apr. 2001 15-June -2001 -140 n gV 1 0.023 mm Hg Stable at ambient room temperature White solid Room temperature; keep tightly closed 3.2.1.2 Test Vehicle The test substance was' methanol. iissolved in spectrophotometic-grade 3.3 Test Conduct _ T h e w ||B H M f l H j P B v a s dissolved in methanol. A Vanan Cary 5 ulttavmleLvisiDle/neaMnTOred spectrophotometer was used to obtain the spectrum. The sample was weighed into a 10-mL class A volumetric flask and brought to volume with spectrophotometric-grade methanol. The solution was shaken and examined visually to ensure that there were no insoluble materials. Two 1-cm cells were triple rinsed with methanol then filled with methanol and placed in the sample and reference beams of the Cary 5 spectrophotometer. A baseline was collected in methanol versus methanol to ensure compensation. The sample cell was then emptied and rinsed three times with sample solution before filling with fresh sample. The sample cell was then placed in the sample beam and the spectrum recorded versus methanol. 3.4 Parameters Observed Changes in molar absorbtivity (M'1cm'1) were recorded versus wavelength (ran). 3.5 Result Analysis The output was compared to the spectrum of methanol versus methanol. Any observable absorption above baseline is noted in the report. Validity Criteria o f the Study The instrument was calibrated for both wavelength accuracy and photometric accuracy 5Telomer Research Program Company Sanitized. Does not contain TSCA CBI 5 Determination of Ultraviolet Spectrum:] 3.7 Protocol Deviations None 4.0 Results and Discussion The ultraviolet absorption spectrum showed no absorption within the wavelength region measured. 5.0 Conclusions oes not absorb ultraviolet radiation in this range. 6.0 Retention of Records Study documents and materials will be stored in the archives of the DuPont Experimental Station including but not limited to: study protocol; any protocol and/or report amendments or addenda or deviations; all raw data; o one original signed copy of the final report; laboratory-specific or site-specific raw data such as personnel files, instrument, equipment, refrigerator, and/or freezer raw data. 7.0 Disposal of Test Item After issuance of the final report, the remaining test substance will be stored at the DuPont Glasgow site until its expiration date and then destroyed by burning, unless other arrangements are made between the sponsor and the Test Facility. 8.0 R e f e r e n c e OECD Guideline for Testing of Chemicals 101, adopted 12 May 1981,UV-VIS Absorption Spectra (Spectrophotometric method). 6Telomer Research Program Company Sanitized. Does no! contain T S C CB1 6 Determination of Ultraviolet Spectrum Figure 1. Ultraviolet Absorption Spectrum of 25C. U V sprectrum for 8 2 Al co ho l at Molar absorptivity (M 1cm 7Telomer Research Program Company Sanitized. Does not contain TSCA CBI 7 Determination of Ultraviolet Spectrum: Appendix A Analysis Summary The subject material was analyzed by gas-chromatography using a standard method to assess its composition. This analysis showed thatjfae material comprised !>fthe subject material. The b a l a n c ^ j J J j w a s n o t| o f quantitation ST0.02%. Further analysis b y 1 suggested that th e ^ ^ B fo 'a s a single impurity, whose chemical structure is Finally, the subject material was evaluated by High Performance Liquid Chromatograpy - Tandem Mass Spectrometry (LC/MS/MS) for the presence o f perfluorooctyl sulfonic acid and perfluorooctanoic acid at a detection limit o f 0. lppm. Neither material was observed to be present. Material Wt. % f+0.3) 8Telomer Research Program Company Sanitized. Does not contain TSCA CBl 8