Document 5RJ90Y4eenq2j2Yb7qqBeDM8

AR226-2978 Du Font HLR 477-92 TRADE SECRET Study Title STATIC, ACUTE, 96-HOUR L.C5Q OF H-19,173 TO FATHEAD MINNOWS. PimephsSespromelas Pata Requirement United States Environmental Protection Agency Environmental Effecte Test Guidelines Subdivision EG-9 Author Kevin N. Baer, Ph.D. Study Completed On Sepf@mber24.1992 Performing Laboratory E. I. du Porrt de Nemoure and Company HaskeSI Laboratory for Toxicology and Industrial Medicine Elkton Road, Post Office Box 50 Newark. Delaware 19714 ^boratorv Project tD Haskeli Laboratory Report No. 477-92 .CampanySanilfead. Does not contain TSC^a ^i- Page 1 of 14 Du Font HLR 477-92 GOOD LABORATORY PRACTICE STATEMENT This study ;was conducted according to EPA RFHA and TSCA Good Laboratory Practice SfaiiGiaMs<4iN3FB 160aRd 4@:CPB 792^^00 Principles of Good Laboratory Practice (C(81)30(Fsna)). Annex 2). and MAPF Japan Good Laboratory Practice Standards (59 NohSan No. 3850). Solubility and stability of the test substance in dilution water and under storage conditions were not determined. Concentrations of the test substance in dilution water were not measured. Protocol amendments and/or SOP deviations appear in Appendix I. Areas of noncompliance (if any) are also documented in the study records. No deviations existed that affected the scientific validity of the study. Submitter: E. I. du Pont de Nemoure and Company Sponsor: Du Pont Chemica's E. 1. du Pont de Nemours and Company STUDY DIRECTOR: &^ //ImM^ fftt//. Kevin N. Baer, Ph.D. Research Toxicologist Environmental Sciences 7/^9^ Mo?Day/Year .Company Sanitized. Does not contain TSCA CCT Du Font HLR 477-92 TABLE OF CONTENTS Page Good Laboratory Practice Statement...................................................^ Tabte of Contenis.<>....,..................................<>.>.............3 General (nformation.,,....,,..............................................................4 Summary..............................................................................."5 QuaCrtyAssurance Documentation ......................................................6 L Purpose........................................................................-7 11 Records.........................................................................7 Ht Materials and Methods ......................................................... 7 A Test Substance.......................................................<>......7 B. Dilution Water...............................................................7 C. Animal Culture ..............................................................7 D. Test Methods................................................................8 E Chemical Analysis ..........................................................8 IV. Results and Discussion.........................................................8 V. Conclusion...................................................................... 9 VI. Literature Cited .................................................................9 VII. TaCbhleemsi.c.a.l..C..h.a.r.a..c.t.e.r.is..ti.c.s..o..f.H..a.s..k.e.i.i.L..a.b..o.r.a.t.o.r.y..W..e..ll...................... 10 10 1. 2. Water Chemistry of the Dilution Water at Test Water............. Start...................... 11 3. Physical and Chemical Characteristics of H-19,173 Test Solutions..... 12 4. Observed Mortality of Fathead Minnows, Pimephales promelas, Exposed to H-19,173 for 96 Hours in a Static, Unaerated Test.......... 13 VIH. ApPproetnocdoilxA.m..e..n.d..m..e.n.t.s..a..n.d...S.O..P.............................................. 14 14 1. Deviations.............................. V^S Ho? COnffiTn -re "- ^ .- Du Font HLR 477-92 GENERAL INFORMATION Mateital Tested: []-------- SimBmgns/Qthfir Caetes- r,B------ Haskajj Mumbar: ftjifty: H-19.173 1^ Composition: ^ OAS Rggistry Num&gr; JSBfiOSSC Study Inmatea/Compieted: h-UfeSty^y ?Ntai9^Cpmpteteti; OuPont Chemicals E.!, du Font da Nemours and Company Witmington, Delaware 19898 4-1-92/9-24-92 4-2-92/6-21-92 r SMitteed. Does hoi eonlain TSCA CB8 4 Du Font HLR 477-92 STATIC, ACUTE, 96-HOUR LC50 OF H.19,173 TO FATHEAD MINNOWS, Pimephates promelas SUMMARY S-M9.173 exhibited low toxicity in a 96-hour, unaerated, static, acute test using fathead minnows, Pfmephafes promelas. Nominal H-19.173 concentrations were 240,340,490,700, and 1000 mg/L. However, undissolved test substance was observed in ail test solutions. The 96-hour LC50, based on nominal concentrations was greater than 1000 mg/L, the highest nominal concentration tested. Due to the lack of solubility, stability, and analytical confirmation of the concentration of the test substance in dilution water, the nominal LC50 should be interpreted with caution. Work By: ^ ^ (f^^sA ^Jeffrey T. Turner, Sr/. Aquatic Associate rr V ^A/fe Mo/Day/Year Work By: fflv^-A^- James W.Wetzel, Aquatic Associate f/^hz Mo/Day/Year THIS REPORT IS APPROVED FOR ISSUE. jft(^ yfZ^- ^ Ke^.v.i--.n..N.>.B-aer, PH.D. Research Toxicologist and Study Director Environmental Sciences 1 ^ / l L Mo/Day/Year Boesffol e'ontan TSCA CBJ Du Pont HLR 477-92 CUALITY ASSURANCE DOCUMENTATION (H-19173) Patf^oftnsMgtiw Conduct-4/3; 6/17/92 Records. Report(s) - 9/17 -18/92 Data Findings reported to: Study Director-9/21/92 Management - 9/24/92 ^U^A Reported hy C.ylcJ'T Donna R. Holt Quality Assurance Auditor Cesnpany Sanitized. Does fibt contain TSCA CBi 6 Du Font HLR 477-92 I. PURPOSE The goal of this study was to assess the acute toxidty of H-19,173 to fathead minnows, Pimephaies proms/as. II. nECORDS Ail data and the final report will be maintained either at Haskell Laboratory for Toxicology and Industrial Medicine (Newark, Delaware) or be archived at the Du Font Records Management Center (Wilmington, Delaware). III. MATERIALS AND METHODS A. Test Substanc^ The test substance is described under Gene at Information. Test solutions were prepared by pipetting the appropriate quantity of H-19,173 and adding it to dilution water (see below). Stability and solubility of H-19,173 in dilution water and under storage conditions were not determined. B. Dilution Water Water (Table 1) originated from the Haskell Laboratory well. C. Animal Culture Fathead Minnows, Pimephales promelas, were reared in continuously flowing well water at Haskell Laboratory. Adult fish resided in 29 L aquaria at approximately 21 C and were fed frozen brine shrimp (Anemia sp.. Golden Gate Brand, Kordon Division, Novalek. Inc., Hayward. CA) generally twice daily, once on Saturday and once on Sunday. Eggs were deposited on polyvinyi chloride tiles coated with sand. Tiles with eggs were aerated and incubated at approximately 25C in a water bath. Larvae were reared in 29 L aquaria at approximately 22C and were fed newly hatched Artemiaso. (Aquarium Products. Glen Burnie, MD) generally twice daily on weekdays and daily on weekends. Juveniles were held either in 272-L circular, fiberglass tanks (23-cm depth; 50 x 25 x 31-cm diameter) or in 29 L glass aquaria at approximately 21 C and were fed newly hatched Anemia sp. and/or frozen brine shrimp generally twice daily on weekdays, once on Saturday and once on Sunday. Sickness, injury, and abnormality were not observed. Animals were identified by labels on aquaria and test vessels. ^iipwsahnte.a.c^'"""""*TM*08' Du Font HLR 477-92 D. Test Methods [1] Five test concentrations and a water control were used. Nominal concentrations were 240,340.490,700, and 1000 mg/L Control fish ranged from 1.2 to 1.8 cm standard length (mean 1.5 cm) and 0.020 to 0.070 g wet weight (mean 0.044 g). Glass aquaria [20 L; 50 (L) x 25 (W) x 31 cm (H)] containing 15 L of test solution (approximately 19-cm liquid depth) were employed. Ten fish were added to each replicate using random numbers (two replicate per concentration, total 20 fish per concentration). Control loading was 0.029 g/L at test conclusion. Test solutions .W ranged from 21.1 to 21 (mean 21.3"C). Fish were not fed 48 hours prior to nor during the test. A photoperiod of 16 hours light versus 8 hours darkness was employed with 25 minutes of transitional light preceding and following the 16 hour light interval. Mortality and behavioral effects were noted daily. Dissolved oxygen (YSS Model 54A, Yellow Springs instruments, Yellow Springs, OH), pH (Coming Model 61 OA, Corning Scientific Instruments, Medfieid, MA), and temperature (American Society of Testing Materials registered mercury thermometer) were measured in the water control and all test concentrations before fish were added at the beginning of the test and daily thereafter. Total alkalinity (Hach kit, Hach Chemical Company, Loveland, CO). EDTA hardness (Hach kit, Hach Chemical Company. Loveland, CO), and conductivity (YSS Model 33. Yellow Springs instruments. Yellow Springs. OH) of the dilution water were measured at the beginning of the test. A continuously-recording thermometer in a control vessel was used to check for temperature variation during the 96-hourtest. E. Chemical Analysis Concentrations of H-19.173 in test solutions were not measured. IV. RESULTS AND DISCUSSION A 96-hour rangefinding test was conducted using nominal concentrations of 1.0. 10,100, and 1000 mg/L (five fish per concentration). No mortalities were observed at any test concentration. A previous definitive study was conducted but was repeated due to excessive control mortality. Ail chemical and physical parameters in the definitive test were within expected ranges (Tables 2 and 3). Nominal concentrations were 240,340. 490,700, and 1000 mg/L. However, undissolved test substance was observed in all test solutions. The 96-hour LC50, based on nominal concentrations and mortality (Table 4) was greater than 1000 mg/L, the highest nominal concentration tested. Due to the tack of solubility, stability, and analytical confirmation of the concentration of the test substance in dilution water, the nominal LC50 should be interpreted with caution. Du Font HLR s 77-92 V. CONCLUSION H-19,173 (undissoived as tested and based on nominal concentrations} exhibited tow toxioty in a 96-hour, unaerated, static, acute test using fathead minnows, Pimephales promelas. The 96-hour LC50, based on nominaj concentrations was greater than 1000 mg/L, the highest concentration tested. VI. LITERATURE CITED 1. United States Environmental Protection Agency, Office of Toxic Substances. Guidelines for Testing Chemicals E6-9, Washington, D.C., August 1982. _.------lc" 9 Du Font HLR 477-92 STATIC, ACUTE. 96-HOUR LC50 OF H-19,173 TO FATHEAD MINNOWS, Pimephales promelas TABLE 1 Chemical Characteristics of Haskel! Laboratory Weii Water1 Parameter Concentration Parameter Concentration BOD. mg/L COD. mg/L DOC, mg/L TOC2, mg/L Total Kjeldahl N, mg/L Ammonia N, mg/L Turbidity. NTU Phenolics, mg/L Color, Co/Pt Solids Total suspended, mg/L Aluminum, mg/L Antimony, mg/L Arsenic, mg/L Beryllium, mg/L Cadmium. mg/L Calcium, mg/L Chloride3- mg/L Chromium, mg/L Cobalt, mg/L Copper, mg/L Cyanide, mg/L Iron3, mg/L Fluoride3 <2 <10 2.8 2.9 <0.2 <0.05 1.6 <0.050 <5 <4 <0.100 <0.060 <0.010 <0.001 <0.002 24.5 6.2 <0.010 <0.020 <0.010 <0.025 0.025 <0.1 Lead, mg/L Magnesium, mg/L MBAS/LAS, mg/L Mercury, mg/L Nickel, mg/L Nitrite3, mg/L Nitrate3, mg/L Phosphate3, mg/L Potassium. mg/L Selenium, mg/L Silver, mg/L Sodium, mg/L Sulfate3, mg/L Sutfide. mg/L Thallium, mg/L Zinc4, mg/L Volatile priority pollutants Acid extractable priority pollutants Base/neutral priority pollutants Pesticides/PCBs Organophosphate pesticides5, ng/L <0.075 3.8 <0.05 <0.00020 <0.020 <0.1 2.1 <0.1 1.8 <0.005 <0.010 8.0 4.8 <0.05 <0.01 <0.020 ND6 ND6 ND6 ND6 <0.50 iDate of sample collection 30 December 1991 unless indicated otherwise, analyses performed at Environmental Testing and Certification Corporation, Edison, New Jersey; ^ate of sample collection 17 June 1992, performed at Du Pont Engineering Test Center, Newark, Delaware; ^ate of analyses 13 January 1992, analyses performed at Du Font Chemicals, Jackson Laboratory, Deepwater. New Jersey; ^elow the Published Method Detection Limit (BMDL); analyses performed at Hazleton Laboratories, Inc., Madison, Wl; ^one detected. contain TSCACBI 10 Du Font HLR 477-92 STATIC. ACUTE. 96-HOUR LC50 OP H-W73 TO FATHEAD MINNOWS, fimephaies Pamelas TABLE 2 Water Chemistry of the Dilution Water at Test Start Total Alkalinity (mg/L as CaCOs) 85 EDTA Hardness (mg/L as CaCOs) 78 Conductivity (umhos/cm) 170 _^---- 1 1 Time (Days) Coritrol A Ba STATIC, ACUTE. 96-HOUR LC50 OF H.19.173 TO FATHEAD MINNOWS. Pimephales promelas TABLE 3 Physical and Chemical Characteristics of H-19,173 Test Solutions 24 0 A B 1Mominail Conceintrations (mg/L) 34 0 49 0 A B A B 70 0 A B Dissolved Oxygen (mg/L) 0 8.6 8.6 8.6 8.6 1 8.6 8.4 8.5 8.6 2 8.5 8.4 8.5 8.5 3 8.4 8.4 8.4 8.4 4 8.4 8.4 8.4 8.4 pH 0 7.4 7.4 1 7.7 7.7 2 7.8 7.8 3 7.8 7.8 4 7.8 7.8 Temperature {C) 7.4 7.4 7.7 7.7 7.8 7.8 7.9 7.9 7.8 7.9 0 21.5 21.6 21.6 21.6 1 21.2 21.3 21.2 21.2 2 21.1 21.2 21.1 21.1 3 21.2 21.3 21.2 21.2 4 21.2 21.4 21.2 21.2 ^ and B represent replicates. 8.6 8.6 8.4 8.5 8.4 8.4 8.4 8.4 8.4 8.4 7.4 7.4 7.7 7.7 7.8 7.8 7.9 7.8 7.8 7.8 21.5 21.2 21.1 21.1 21.1 21.6 21.2 21.2 21.1 21.1 8.6 8.6 8.6 8.5 8.5 8.4 8.4 8.4 8.4 8.3 7,4 7.4 7.7 7.7 7.8 7.8 7.8 7.9 7.8 7.8 21.6 21.2 21.1 21.5 21.4 21.6 21.3 21.1 21.3 21.3 8.6 8.6 8.6 8.6 8.5 8.4 8.4 8.4 8.4 8.4 7.4 7.4 7.7 7.7 7.8 7.8 7.8 7.8 7.8 7.8 21.5 21.2 21.1 21.3 21.4 21.5 21.2 21.1 21.4 21.4 1 A 8.6 8.6 8.4 8.4 8.3 7.4 7.7 7.8 7.9 7.9 21.5 21.2 21.1 21.5 21.4 Du Font HLR 477-92 STATIC, ACUTE. 96-HOUR LC50 OF H-19,173 TO FATHEAD MINNOWS. PimephaSes promelas TABLE 4 Observed Mortality of Fathead Minnows, Pimephales pmmelas, Exposed to H-19,173 for 96 Hours in a Static. Unaerated Test Nominal Concentrations (mg/L) HgO Control 240 340 490 700 1000 24 H(surs A B 0/10 0/10 0/10 0/10 0/10 0/10 0/10 0/10 0/10 0/10 0/10 0/10 Cumuleitive Modlaiity in PiBrcent 48 Heurs A B 72 H(3urs A B 0/10 0/10 0/10- 0/10 0/10 0/10 0/10 0/10 0/10 0/10 0/10 0/10 0/10 0/10 0/10 6/10 0/10 0/10 0/10 0/10 0/10 0/10 0/10 0/10 96 H(surs A B 0/10 0/10 0/10 0/10 0/10 0/10 0/10 0/10 0/10 0/10 0/10 0/10 sA and B representreplicates; each replicate contained 10 fish (total 20 fish per test concentration). DoesnoloontalnTSCACBl Company ^Sannil^wedo-" 13 Du Font HLR 477-92 STATIC, ACUTE, 96-HOUR LC50 OF H-19.173 TO FATHEAD MINNOWS, Pimsphales prwnelas APPENDIX 1 PROTOCOL AMENDMENTS AND SOP DEVIATIONS PROTOCOL AMENDMENTS 1. Analytical samples wiif not be taken. REASON FOR CHANGE: Sponsor request. 2. TRhEeASSpOoNnsFoOr'Rs aCpHpAroNvGalEd: aDteatise 8in-a1d7v-9e1rt.ently omitted in the STSPS. SOP DEVIATIONS 1. TOC samples were inadvertently not taken in the first definitive test. 2. The photoperiod (Lux) was inadvertently not measured in the second definitive. ,^Ooesnolcon..n.SCACB Compaq San^d.D 14