Document 2mGdNVwxYdkXj3O9m6bVb5ra

AR226-3366 lllll1ii IM w m Bm H T?s, o ^ ^ ttm m 9 m T.L. Siotmuu R.A. //< lllfll ABSTRACT METHODS AND MATERIALS ?li^P naroafc surfssfeajtwaa-ItiGmg/L, 'WlJ-waniwui ssrfectaaAWs sS^^incwtoxsft, *<w tfl* hMWl'bett. te to w a# t>F* o tto * f t a , S$0 10? B%&, NOEC t ffig?L5bfaonw<*r(o3! romd teis?ux > Arfidytrca \critcB & !tt tJf iba-fluorofekrare: pKtspBio: s s d treo^feto -a&etant. vuoccnCwfiun as INTRODUCTION ......................... ^ijjjiisiijirej&ijiiicj;^ 8also 'opuriaal for d a compoujils tliat mayreach aquatic ecosystems, W 5 tfosei f o p 9Bk gflap t f m i y p x i kjr&OMX h a caaeenEistwm, uf repKfKntofcvfr estera licre representa! ka the toad ccnaenCrahon e f tbc tcsaL fcfeww* i ^ f c t a n M i V ^ ^ W M r o t e M ^ ^ ,^ .fla .sS lw ,l^ f< ^ ,.3 )3A^rSAd<6,7<? A ^ ^ J ^ ^ . ^ d ^ ^ w ^ W . W J . ^ ! J P , . C f c J W : ^ ) ; . , , , , , , . Wftttl <stcr i-Otaaoj, 3<3,^,-L 5,6,$,717,8,8,8 ?n<}&fusyo-, j-piopstw l,}jydwgsft phostrtwte 3ctt ::jl&::fl3i:|;v^S::!:i|S:|i!!!j;^ ' TTC58Sa5 -.... " U , " , TM " "" S IE E E iM E : Flnoroteloracr ethoxylate surfecant (H-24768). is amxture o ethcscylatesxdcriyed from Telomet B : Alcohcs (TBA) o f the fbHowing:sttucture: CFj-(GF2)B-CH2-CH2-0^(CHyCH2-0 )l-H, wBc n=3, 5 7 9, l i , 13,... and ,,* 0-25 (Figure 2). The mjoreomponcnts of the mixturo arc derived for lhree:TBAs {6-2 TBA, ,a=5; 8-2 TBA,a=7; 10-2 TBA, =9); Five of the c o s t intense mdividual ethoxylatos were selected fo t analysis for each o f the TBAs. For 6-2 TBA, 8-2 TBA, and 10-2'TBA, the nibst intense Svc cflioxylhtcs represented ,=7-11. * Quantitation was based on the total signal obtained for the live selected ethoxylates for each TBA. Separate calibration curves were made for each group of TBA cthoxylates. The content of each of the selected cthoxylates in the tesfsubstance was not known. The total concentration o f the test substance was assigned to each group o f cthoxylates that were monitored. Figure 2. Test Substance (H-24768) Chromatogram (A) and example spectrum of the 6-2 TBA cthoxylates present in the peak at 33 min (B). nrchck.J., M. Zeeman and R. ClemcMa. 1995. P-eotoxieologyanil the usesmem ofchcrr^lsat HieU.S. EPA Office of Pollution Prevention and Tonics: Current Activities and Puture Seeds, pp. 27-158 Ja: Mating EnMrertmaitScloiee. J.R. Pratt, N. Bowere andJ.R. Stauffer (Ed*.). Eecprinl,Portland,OR. 27! pp. w W nAi S i *w>s 4 <$ " i 1" 1 1 ^P W i^. I S p & W 0 $ %f ^ mMSM I ^ p WmM ' *>, : | lllip p li W w 92 I* * * ! 72-HouAd ttioor !m Curve(Meent SD) SCOOMM eowoooo T / [....... H' 4000 * 300K800 ________________ ~ -- T = z n-------------- -- 1j t - ---------------- -- -- : i fominal ConMfttmUon,mefL |..~ ManAUC-H-24678-*-MsnAU5.VW506 72-HourGrowth Rate(Meani SD) Mean 72-Hour Col Count (Mean SD) '' / ''J ---------- \ ^ ....................- 4 .................... .. Ji *j I Nominal Concentration, mart. | | - -MeanCetCourt-H-g4678 *-HaanCilCci>rt-H-2S435l ECSfcrodm E C Vafees(mg/L) Camperos ' 'CettlS! AUC Crrw ihRB^ &ms 5C5D NQFC >W >100 >100 |i|l|||| >m >100 JJ-24T68 EC50 'NOEC >100 *? - aOO >100 25 ' ; 5 ./'". Lz.rr^.'^"r" The acute and chronic hazard to algae oftw o.fluorotelomer surfactants was assessed during 72-hour regulatory guideline tests conducted using Good Laboratory Practices. ECSOs based cminhibition of healthy cell count, area under the growth curve, and growth rate were greater than 100 mg/L for both compounds. The lowest 72-hour NOEC for the anionic surfactant was 25 mg/L while the 72-hour NOEC for the nonionic surfactant was 100 mg/L. Although the anionic surfactant was slightly more toxic, test results suggest both substances are of low concern (i.c., EC50> 100 mg/L, NOEC > 10 mg/L) fear acute and chronic hazard to algae (SmrcheketaJ. 1995).