Document 2RxxLkQerxGkem1Bjkam7gV4R

AR226-2824 /c - /9 > - 5 ~ International Research and Development Corporation SPONSOR: E.I. duPont de Nemours and Company MATERIAL: - SUBJECT: Ninety-Day Feeding Study in the Rat.- Development Corporation Collaborators: R. H. Buller, Ph.D., Director of Pharmacology R. G. Geil, D.V.M., Director of Pathology Date : November 30, 1965 SSoinpany Sanitized. Does not contain TSCA CB1 International Research and Development Corporation Page 1 I. SYNOPSIS In a 90-day feeding study, male and female albino rats were fed diets containing ^ H ^ t levels of 100, 500 or 2500 ppm. After 35 days of continuous'feeding, the 500 and 2500 ppm. dietary levels were increased to 1000 and 5000 ppm., respectively for the remainder of the study. After the prescribed 90-day period of com pound administration, representative animals were placed on a with drawal study. All rats appeared essentially normal with respect to behavior and appearance throughout the study. No adverse effect on body weight gain was found at any dietary level employed in this study, both in the active compound administra tion phase and in the withdrawal period. Average total weekly food consumption measured in grams/rat/week in those groups fed 100 and 500 - 1000 ppm. the diet compared favorably with the control rats throughout the study. At the 2500 - 5000 ppm. dietary level, food consumption of the male raps ranged from 1.1 to 8.7 per cent less than control male rats, and food consumption of the female rats ranged from 5.2 to 16.4 per cent less than the female control rats. These differences were first noted in the 8th week for males and in the 4th week for females and continued throughout the treatment period. No meaningful differences in food consumption were reflected by the treated groups of rats in comparison to the control group on the basis pf grams of food consumed per day per kilogram of body weight. No compound-related hematologic or biochemical changes were found Company Sanitized. Does not contain TSCA CBJ International Research and Development Corporation i i i! Page 2 i at the 100 and 500 - 1000 ppm. dietary levels of ^ However, slightly decreased values for erythrocyte counts, hematocrits and hemoglobin concentrations were found for males and females at the 2500 - 5000 ppm. level, particularly at the terminal (90-day) clinicopathology examination. Urinalyses were normal at all times. Compound-related changes observed at the 90-day necropsy examina tion consisted of increased liver and kidney weight at the 1000 and 5000 ppm. dosage levels and pale yellowish livers in some male rats from the 500 - 1000 and 2500 - 5000 ppm. dosage levels. In histologic section, only livers from the 2500 - 5000 ppm. dosage level showed any change and this consisted of a slight hypertrophy of centrolobular hepatocytes. The increase in l^ver and kidney weights and centro lobular hepatocyte hypertrophy persisted with diminished magnitude through 21 days of compound withdrawal. Similar organ weight and histologic changes were observed at the 30 and 60-day interim sacri fices . Company Sanitized. Does not contain TSCA CBf international Research and Development Corporation Page 3 II. COMPOUND The test compound was received from E. I. duPont de Nemours and Company, Wilmington, Delaware, on June 19, 1965. It was a brown amorphous solid in containers bearing the labe] Haskell No. 4212." Company Sanitized. Does not contain TSCA CB> international Research and Development Corporation Page 4 III. CLINICAL STUDIES: A. METHODS: 1. General Procedure: Eighty male (weighing from 45 to 64 grams) and eighty female (weighing from 47 to 63 grams) albino rats of the Charles River strain were used for this study. The rats were housed individually in cages suspended above the droppings in an air-conditioned room throughout the study and were fed a diet of Purina Laboratory Chow for rats ad libitum. Water also was available at all times. The animals were divided into one control group and three treated groups of 20 male and 20 female rats each. The rats in each sex group were selected so that the average body weight of each group was similar to that of the other groups of the same sex. 2. Compound Administration: incorporated into the standard powdered laboratory diet of Purina Laboratory Chow and offered to the treated groups of rats ad libitum. The test diet was freshly prepared each week and the compound-in-diet levels mixed so that the rats received^[|^|^^at dietary levels of 100, 500, or 2500 ppm. In the sixth week of com pound administration those groups receiving 500 or 2500 ppm. were increased in concentration to dietary levels of 1000 or 5000 ppm., respectively. Those animals receiving 100 ppm. o f ^ m | ^ i n the diet continued to receive this level throughout the 13-week study period. The control groups of rats received the powdered diet of Purina Laboratory Chow, but without Company Sanitized, Does not contain TS CA CBf Inzernational Research and Development Corporation Page 5 Following 13 weeks of compound administration rats in all groups were sacrificed and subjected to necropsy examination with the exception'of certain selected animals from the control`group and from the treated groups at the 1000 and 5000 ppm. dietary levels which were continued on study in a compound withdrawal phase. The withdrawal phase of this study will: be reported in its entirety in a subsequent and separate report. 3. Observations: The control and test animals were observed daily for mortality, alteration in general appearance and behavior, and signs of pharmaco dynamic and/or toxic effects. Body weights, food consumption, and food efficiency values were recorded for each rat weekly throughout the study. 4. Laboratory Tests: a . Hematology: Hematologic examination consisted of erythrocyte counts, 1. 2T total and differential leucocyte counts, hematocrits , and hemoglobin concentrations. These studies were performed individually on 6 male and 6 female rats randomly selected in the control and each test group during the control period and again at 30, 60, and 90 days. b . Urinalysis: Urine samples were obtained from the same animals at the same time intervals used to obtain blood for hematology. Urinalysis Coulter Particle Size Counter, Model A., Coulter Electronics, 2 590 W. 20th Street, Hialeah, Florida. Miller, S., Microcapillary Method, Textbook of Clinical Pathology, 1960, Williams and Wilkins Company, Philadelphia, Pa., p. 43. 3 Miller, S., Cyanmethemoglobin Method, Textbook of Clinical Pathology, 1960, Williams and Wilkins Company, Philadelphia, Pa., p. 35. v , -"any Sanitized. Does no! contain TSCA C~!? International Research and Development Corporation Page 6 consisted of qualitative tests for g l u c o s e , b i l i r u b i n ^ , occult b l o o d , a n d albumin,^' measurements of volume, and specific gravity, and microscopic examination of the urinary sediments. c. Biochemistry: Biochemical examinations were conducted at the same inter vais as for hematology. Serum transaminase (SGOT and SGPT) . and' plasma alkaline phosphatase determinations^ were performed on 6 male 4 "Combistix" 5 "Clinistix" 6 "Clinitest" (Ames Reagent Strips). (Ames Reagent Strips). (Ames Reagent Tablets). 7 "Ictotest" (Ames Reagent Tablets). 8 "Heraastix" (Ames Reagent Strips). 9 "Hematest" (Ames Reagent Tablets). 10 "Occultest" (Ames Reagent Tablets). 11 "Albustix" (Ames Reagent Strips). 12 "Bumintest" (Ames Reagent Tablets). 13 Heller's Ring Test, Practical Physiologic Chemistry, Hawk, Oser and Summerson, 13th Ed., p. 830. 14 Beckman Expanded Scale pH Meter, Model No. 76. 15 Reitman, S., and Frankel, S., Colorimetric Method for the Determina tion of Serum Transaminase Activity, Am. J. of Clin. Path., 8: 56, 1957. ^ Marsh, W., Modified King-Armstrong Method, Clin. Chem. 5: 119, 1959. npany Sanitized. Does not contain TSCA CBI international Research and Development Corporation Page 7 and 6 female rats randomly selected from the control and treated groups. The animals chosen for hematology values were not used for these bio chemical determinations. B. RESULTS: 1. General Behavior and Appearance: No adverse changes in behavior or appearance were encountered that could be related to the administration Animals in the control and all treated groups appeared essen tially normal each day with the exception of an occasional rat in each group that exhibited slight nasal and/or ocular porphyrin discharge. Other incidental findings, unrelated to compound administration, included one treated female animal (Rat #14374) at the 5000 ppm. dietary level which exhibited a swollen nose in the 13th week of study, one treated male (Rat #14298) at this dietary level which exhibited a mass on the flank from the 16th week (withdrawal period) until terminal necropsy examination, and one treated male (Rat #14324) at this same dietary level which exhibited destruction of the right eye, from the 15th week to the terminal (in the withdrawal period) necropsy examina tion. 2. Body Weights (Tables 1-8 and Figures 1 and 2): a. Control: The control animals maintained body weight curves which were consistent with those curves exhibited by control animals of the same age and strain maintained in these laboratories from time-to-time. b. 100 and 500 ppm.*: Male and female rats at these dietary levels maintained body weights which paralleled closely those of their respective control groups. * 500 ppm. dietary level increased to 1000 ppm. in the 6th week of study. zmpany Sanitized. Does not contain TSCA CBS International Research ana Development Corporation i Page 8 I j c . 2500 ppm.** : No marked body weight changes occurred among male and female rats at this dietary level during the course of compound administration. Male animals in the 9th week exhibited a body weight gain 8.8 per"cent less than control males. This difference in body weight gain persisted for the duration of the study period. During the withdrawal phase of this study the greatest decrease in body weight gain occurred. Even then, however, this difference was only about.10 per cent less than that of the male control animals. Female treated animals in this group in the 7th week of study exhibited a weight gain which was 11.5 per cent less than that of the control female animals. This difference in body weight gain per sisted for the duration of the treatment period. The greatest differ ence in body weight gain of the female group was noted in the 12th week of study at which time a difference of only 11.7 per cent occurred. 3. Food Consumption (Tables 10 and 11): a. Grams/Rat/Week: Average total weekly food consumption for male and female rats in those groups receiving 100 ppm. and 500 ppm.* compared favorably with similar measurements obtained from the control group. Treated rats receiving 2500 ppm.** showed food consumption values less than those of control animals beginning in the 4th week for treated females and in the 8th week for treated males. This decrease in food consumption continued throughout the study period and ranged from 1.1 to 8.7 per cent for the males and 5.2 to 16.4 per cent for the females in this group. The decreased food consumption in this * 500 ppm. dietary level increased to 1000 ppm. in the 6th week of study. ** 2500 ppm. dietary level increased to 5000 ppm. in the 6th week of study. Company Sanitized. Does not contain TSCA CBI international Research and Development Corporation r Page 9 group continued in both sexes for the duration of the treatment period. b. Grams/Kg,/Day: - No biologically meaningful differences were observed on food consumption in the treated groups of rats when compared with the control group on a basis of grams/kg./day food consumed. 4. Survival (Table 9): Other than for those animals subjected to interim necropsy examination at 30 and 60 days, all control and treated animals survived the course of study with two exceptions. One control female (Rat S) #14160) succumbed in the terminal (13th) week of study and one treated male (Rat #14212) at the 100 ppm. level of succumbed in the 11th week of study. 5. Laboratory Tests: a. Hematology: No compound-related hematologic changes were found at the 100 and 500 ppm. dietary levels At the 2500 ppm. level, group values for both sexes, with respect to erythrocyte count, hema tocrit and hemoglobin concentration, generally were slightly lower than those for the control animals and rats at the 100 and 500 ppm. dietary levels of though some changes in these parameters were seen at the 60-day interval of examination, they were overall more pronounced after 90 days of compound administration. It is of interest that inspection of these values for individual rats in the high dietary level groups failed to reveal marked changes for any given animal, that is, whereby that animal's value would tend to markedly lower the group average, but rather that lower values, with a relatively small spread from individual to individual, were found for most of these rats. Company Sanitized. Does not contain TSCA CUt International Research and Development Corporation Page 10 Group average values are summarized for male rats in Table 12 and for female rats in Table 13. Individual values for all male and female rats appear in Tables 14 through 17. . b. Plasma Biochemistry: No compound-related changes were found at any period of examination with respect to serum alkaline phosphatase activity or serum glutamic pyruvic transaminase (SGPT) or serum glutamic oxalacetic transaminase (SGOT) activities. Values obtained in these studies appear in Tables 18 through 21. c. Urinalysis : Urinalysis examinations failed to reveal changes which were considered to be related to treatment with the test compound. Results of these measurements appear in Tables 22 through 25. Company Sanitized. Doss noi contain TSCA C M Iniernaiional Research and Development Corporation Page 11 IV. PATHOLOGICAL STUDIES A. METHODS: 1. Gross Examination: After 30 and 60 days of compound administration, 3 male and 3 female rats from the control and each treated group were sacri ficed by exsanguination and subjected to necropsy examination. After 90 days of compound administration, 10 male and 10 female rats from the control, 1000, and 5000 ppm. dietary level groups and all surviving rats from the 100 ppm. group were sacrificed by exsanguina tion and subjected to necropsy examination. Three male and 3 female rats from the control, 1000 and 5000 ppm. groups were sacrificed and subjected to necropsy examination after a 21-day compound withdrawal . period. (Other rats that remained on withdrawal beyond 21 days will be reported on in a separate report.) At necropsy major organs were weighed and representative tissues from each rat were collected into 10 per cent neutral buffered formalin for subsequent histologic processing. At the 90-day sacrifice, specimens of brain, liver, kidneys, muscle, fat, spleen, testes and blood were pooled by sex and diet^jry group, frozen and forwarded to the sponsor. Specimens of liver from the interim and withdrawal sacrifice were also pooled by sex and dietary group, frozen and shipped to the sponsor. Rats which died on study were also subjected to necropsy examination unless this was precluded by advanced autolysis. 2 \ Microscopic Examination: The following tissues from each of 3 male and 3 female rats from the control and high dosage groups from the 30 and 60-day interim and 21-day withdrawal sacrifices and from each of 10 male and 10 -.Dossgo!containTSGi.S International Research and Development Corporation Page 12 female control and high dietary group rats from the 90-day terminal sacrifice were paraffin-embedded, sectioned, stained with hematoxylin and' eosin and examined microscopically: brain spinal cord peripheral nerve eye pituitary thyroid parathyroid adrenal lung heart spleen lymph node thymus bone marrow salivary gland stomach small intestine large intestine pancreas liver kidneys urinary bladder testes or ovaries prostate or uterus skeletal muscle skin bone Sections of liver from 10 male and 10 female rats from the 1000 ppm. level - 90-day sacrifice rats were also processed as above and examined. B. RESULTS: ` 1. Gross Pathology (Table 26) and Organ Weights (Tables 2.7 and 28): Compound related gross changes observed at necropsy were limited to male rats from the 1000 and 5000 ppm. dietary level groups and consisted of pale, yellowish livers in some but not all male rats from the 5000 ppm. dietary level group and in a few rats from the 1000 ppm. dietary level group. None of the rats dying on study died of compound related causes. Rat #14160 (Control) died of pneumonia. Autolysis precluded diagnostic necropsy of Rat #14212 (100 ppm.). Compound related variations in organ weights were limited to the livers and kidneys of treated rats. At the 90-day sacrifice there was a moderate increase in actual and relative liver weights of the 1000 and 5000 ppm. dietary level rats. This increase was also seen in the 5000 ppm. dietary level rats at the 60-day interim Company Sanitized. Does not contain TSC CBii International Research and Development Corporaiion Page 13 sacrifice and in the 2500 ppm. dietary level rats at the 30-day interim sacrifice. After 21 days of compound withdrawal, a slrght increase in*liver weight persisted at the 5000 ppm. dietary level. - Mean actual and relative kidney weights were slightly^m the 1000 and 5000 ppm. dietary level rats at the 90-day sacrifice. Kidney weights were also slightly increased in the 1000 and 5000 ppm. level rats at the 60-day interim sacrifice and 21-day withdrawal sacrifice and in the 500 and 2500 ppm. level at the 30-day interim sacrifice. Although the values from the interim and withdrawal sacrifices represent only 3 rats per sex group, these variations in kidney weights always had a dietary-level relationship. 2. Histopathology (Tables 29 and 30): Compound related histopathologic changes were found only in the livers of rats from the highest (2500-5000 ppm.) dietary level and consisted of slight hypertrophy of centrolobular hepatocytes. Affected liver cells had cytoplasm which was less coarsely granular and more homogeneous than the unaffected cells at the periphery of the liver lobules and in the livers of rats in the control and lower dietary levels. This change, to a slight degree was seen after 30 days at the 2500 ppm. level. After this group was raised to 5000 ppm., the change was more marked at the 60 and 90-day sacrifices. A very slight change persisted in the 5000 ppm. level male rats sacrificed after a 21-day compound withdrawal period. This liver change was always more marked in male rats and was seen only at the highest (2500-5000 ppm.) dietary level. No lesions in other organs were considered to have been of compound related origin. No histologic basis was found for the slight increase in kidney weights in treated rats. oany Sanitized. Does not contain TSCAGBli Ninety-Day Feeding Study in the Rat. t~ / 0 - r 9 a - 'T" 14142 14143 14144 14145 14146 14147 14148 14149 14150 14151 14152 14153 14154 14155 14156 14157 14158 14159 14160 14161 Me an 55 93 54 68 56 99 56 83 56 94 55 91 57 88 60 101: 55 72 58 83 56 67 50 52 57 63 57 71 51 67 58 64 57 73 58 54 50 76 54 48 55 75 129 95 134 107 136 123 124 135 103 116 114 93 82 116 115 105 106 99 124 91 149 175 123 136 163 187 130 142 161 184 141 160 149 170 162 177 126 149 137 158 133 154 122 138 117 141 142 160 139 161 121 134 132 153 146 156 147 162 128 148 185 144 207 159 196 179 178 199 164 181 171 143 156 170 161 154 149 181 157 144 195 155 249 166. 217 184 197 215 191 189 189 166 172 192 196 153 172 199 189 178 Sacrificed 169 191 260 285 Sacrificed Sacrificed 191 231 214 249 230 261 213 243 202 237 203 235 183 231 195 237 203 239 210 243 166 165 190 232 219 240 206 221 192 231 198 296 218 241 265 243 224 226 207 225 223 241 174 244 232 215 214 Sacrificed 306 314 221 236 252 268 Sacrificed 252 271 224 234 Sacrificed 216 248 233 246 244 258 250 261 178 185 215 236 250 264 239 256 226 236 112 138 157 169 188 203 234 229 236 251 Company Sanitized. Does not contain TSCA CBS O0Q> TABLE 1. Rat Number Ninety*Day Feeding Study in the Rat. Continued. Individual Weekly Body Weights, Grams. 1i --------- -- -- _ ..........Compound Administration Weeks LjL 1J 14* 15 16 17 18 Control - Female : 14144 14147 14148 14150 14151 14153 14154 14155 14156 14157 14158 14159 14160 14161 14313 320 326 332 244 252 332 271 285 267 285 288 233 241 251 242 260 248 244 256 263 241 265 271 238 275 283 259 200 200 191 245 277 265 253 281 271 231 282 Died 250 289 264 299 266 310 Sacrificed Sacrificed 241 251 257 261 268 276 Sacrificed 242 249 251 256 255 Mean 260 266 258 261 270 276 256 255 Initiation of withdrawal period (14th week), 19 254 254 aniti'Gtf ;oesnot c ntiain rC> C&t Page 15 Ninety-Day Feeding Study in the Rat TABLE 2. Individual Weekly Body Weight s, Grams. Rat Number Control Period 12 12 Control - Male: 14162 14163 14164 14165 14166 14167 14168 14169 14170 14171 14172 14173 14174 14175 14176 14177 14178 14179 14180 14181 64 62 60 59 55 50 61 45 58 51 60 52 51 54 57 54 62 52 53 55 99 97 100 94 92 67 98 74 95 83 83 55 64 76 76 71 64 66 67 67 143 185 151 207 89 169 157 214 133 175 103 145 147 189 113 143 136 185 119 158 152 205 105 153 113 155 137 185 136 180 123 167 117 153 130 181 120 172 128 177 Mean 55 79 128 175 ^OTOpany Sanitized. Does not contain TSCA / * ' Compound Administration Weeks 3 4 5 6 7 8 9 10 230 244 263 Sacrificed 256 229 251 294 288 309 309 324 346 338 354 366 388 Sacrificed 388 446 402 461 412 480 211 248 274 300 321 344 360 37 0 187 231 260. 290 311 308 Sacrificed 243 276 308 Sacrificed 224 231 191 274 265 260 307 285 257 337 317 287 365 348 310 39.5 413 433 380 Sacr ificed 349 364 390 263 307 300 336 371 417 441 467 199 242 263 291 306 334 346 370 204 238 273 318 341 373 382 412 234 222 251 264 293 294 317 328 370 354 422 390 449 393 469 420 209 241 271 300 318 336 341 358 200 '234 250 283 311 340 Sacrificed 231 238 261 322 347 380 395 420 224 272 300 334 360 388 398 423 234 241 300 344 373 401 409 441 224 261 287 318 344 376 397 420 O0Q3 fO ON Ninety-Day Feeding Study in the Rat. TABLE 2. Rat Number Continued. Individual Weekly Body Weights, Grams. Compound Administration Weeks 11 12 13 14* 15 16 17 18 Control - Male : 14163 14164 14166 14169 14171 14172 14173 14174 14175 14176 14177 14179 14180 14181 14314 14315 406 391 375 505 505 495 398 409 383 446 453 431 412 423 385 488 483 478 389 405 376 427 440 401 498 496 506 442 452 425 365 374 357 396 402 414 Sacrificed 435 455 461 479 489 500 Sacrificed 415 463 461 489 472 514 Sacrificed 451 463 472 476 466 497 506 515 538 518 556 574 571 512 503 523 539 544 Mean 434 444 429 482 475 501 540 543 * Initiation of withdrawal period (I4th week). 19 522 585 559 555 eompan, Sa"f e d -Doe, no, TSCA cp Page 17 Ninety-Day Feeding Study in the Rat. TABLE 3. Individual Weekly Body Weight, Grams. Rat Number Control Period 12 12 Compound Administration Week? 3 4 5 6 7 8 9 10 100 ppm. - Female: 14182 14183 14184 14185 14186 14187 14188 14189 14190 14191 14192 14193 14194 14195 14196 14197 14198 14199 14200 14201 56 86 51 77 59 82 55 87 57 101 55 76 55 80 55 63 55 88 53 84 54 60 53 67 61 73 59 69 57 65 50 58 62 76 51 60 53 70 59 65. 112 131 151 167 175 Sacrificed 123 113 110 153 171 126 145 135 154 203 165 174 210 182 186 231 250 194 211 Sacrificed 262 218 273 285 Sacrificed 144 109 111 176 210 134 155 124 139 252 175 149 251 194, 162 275 290 206 230 Sacrificed 303 245 307 252 331 269 91 116 122 106 113 .90 107 105 .98 123 102 123 115 119 150 137 154 153 177 127 141 141 158 124 148 133 157 147 159 121 146 141 167 125 141 147 165 152 179 168 176 187 172 181 160 175 179 157 195 144 184 183 177 180 208 174 199 179 190 183 179 202 180 197 208 198 200 223 185 210 203 211 199 197 220 197 212 235 216 211 241 193 222 221 231 213 207 237 215 224 245 230 225 258 207 238 234 245 227 227 253 236 244 263 245 257 225 242 269 286 215 234 Sacrificed Sacrificed 243 ' 265 236 267 238 258 259 270 241 256 246 264 271 275 Mean 56 74 112 137 158 177 191 212 227 242 251 269 Company Sanitized. Does no! contain T S C A CB! TJ o ce Ninety-Day Feeding Study in the Rat. 14183 14186 14187 14189 14190 14191 14192 14195 14196 14197 14198 14199 14200 14201 Mean 290 306 283 3'45 353 333 271 285 258 272 274 252 248 255 227 287 303 283 236 248 221 280 286 261 277 276 247 251 262 244 283 296 278261 269 273 273 278 285 298 301 308 277 285 268 Does no! contain T S C A CBI Page IS Ninety-Day Feeding Study in the Rat TABLE 4. Individual Weekly Body Weight, Grams Rat Number Control Period 12 100 ppm. - Male: 14202 14203 14204 14205 14206 14207 14208 14209 14210 14211 14212 14213 14214 14215 14216 14217 14218 14219 14220 14221 58 51 62 55 63 54 55 55 60 54 50 53 61 63 53 49 58 55 61 60 Mean 57 79 76 87 86 103 85 81 88 86 87 60 81 83 83 70 58 64 73 68 74 79 1 119 168 116 157 149 203 139 189 160 215 124 165 120 159 128 166 142 197 134 173 108 122 116 161 149 205 136 171 126 164 97 135 100 142 121 155 110 136 135 176 127 168 ________ Compound Administration Weeks 3 4- 5 6 7 214 246 :270' Sacrificed 201 224 261 296 328 256 310 343..' 371 407 242 275 286 Sacrificed 255 298 320 346 373 209 248 282, 306 338 199 211 228 Sacrificed 209 244 254 269 301 252 303 330 354 388 217 251 284 312 339 130 168 211 250 256 195 238 243 271 290 267 284 326 352 377 210 250 259 267 289 217 271 308 247 377 168 186 219 260 286 199 247 286 330 369 207 216 275 310 333 196 213 264 287 307 227 255 281 308 331 214 252 277 308 335 8 356 448 403 363 327 398 369 296 305 399 320 407 316 398 358 329 357 362 9 10 376 400 467 495 405 434 378 402 347 372 417 454 385 410 317 300 308 335 407 441 349 375 420 457 Sacri ficed 321 454 364 393 Sacrificed Sacrificed 376 409 igRifed: Bees net contain TSC CBf Ninety-Day Feeding Study in the Rat. TABLE 4. Continued. Rat 11 100 ppm. - Male: 14203 14204 14206 14207 14209 14210 14211 14212 14213 14214 14215 14216 14218 14219 411 521 450 423 385 462 432 Died 353 446 385 482 473 406 Individual Weekly Body Weight, Grams. Compoiand Administration Weeks 12 13 14 15 16 17 440 427 527 515 468 432 436 410 398 363 477 481 456 432 368 348 456 448 405 383 501 500 496 507 421 430 Mean 433 450 437 18 19 * Wffeetf Dna Does tsca CBl Page 21 Ninety-Day Feeding Study in the Rat TABLE 5. Individual Weekly Body Weight, Grams. Rat Number Control Period 12 123 500 ppm. - Female:* 1.4222 14223 14224 14225 14226 14227 14228 14229 14230 14231 14232 14233 14234 14235 14236 14237 14238 14239 14240 14241 55 60 58 48 57 57 55 55 55 52 50 52 59 58 56 52 50 57 55 55 88 83 92 59 73 92 86 78 90 76 57 64 73 68 76 52 77 74 75 72 126 153 116 134 111 127 '91 115 115 140 130 157 130 154 113 140 119 134 114 153 100 134 105 125 110 129 119 148 117 150 86 123 119 150 129 146 117 138 100 128 177 154 141 136 161 178 166 160 147 184 147 130 140 175 171 145 171 163 154 144 Mean 55 75 113 139 157 Compound Administration Weeks 4 567 8 190 204 Sacrificed 172 181 195 202 213 163 185 200 212 238 141 154 Sacrificed 182 188 204 215 223 201 210 .. 230 244 256 178 197 Sacrificed 174 184 202 215 237 158 175 190 202 215 195 219 237 254 272 174 186 204 216 234 148 160 170 181 191 140 164 176 187 195 201 214 255 249 266 193 213 216 236 242 146 168 188 200 212 195 199 211 222 227 183 198 206 221 234 157 188 197 214 224 143 180 188 200 206 172 188 204 216 229 9 10 Sacrificed 273 260 235 247 264 276 254 261 Sacrificed 287 303 241 253 201 204 Sacrificed 279 297 248 263 221 223 236 243 242 253 230 245 216 236 243 255 Dosage level increased in the 5th week of study to 1000 ppm. Company Sanitized. Does not contain TSCA CBS nPid TABLE 5. Ninety-Day Feeding Study in the Rat. _500 ppm, - Femaie 14224 14226 14227 14229 14231 14232 14233 14235 14236 14237 14238 14239 14240 14241 14306 14308 14320 Mean 267 279 263 246 257 239 244 282 269 265 277 270 310 314 268 255 269 251 212 216 207 304 312 299 262 272 254 232 244 234 253 259 256 235 256 266 263 245 242 261 259 246 263 262 274 254 276 272 278 269 278 279 280 267 Sacrificed Sacrificed Sacrificed 269 282 276 254 266 270 272 284 284 249 258 266 265 273 283 234 236 241 246 250 253 257 268 254 256 265 269 263 272 274 Company Sanitized. Does no! contain TSCA CBl Ninety-Day Feeding Study in the Rat. TABLE. 6. Individual Weekly Body Weight, Grams. Rat Number Control Period 12 500 ppm. - * Male: ' 14242 1424.3 14244 14245 L4246 14247 14248 14249 14250 14251 14252 14253 14254 14255 14256 14257 14258 14259 142 60 14261 58 85 57 92 56 78 63 96 56 63 58 72 55 83 62 90 58 85 52 75 63 89 62 78 55 75 57 70 55 67 53 81 56 98 52 63 58 69 59 79 124 155 185 136 178 226 134 196 249 136 172 213 91 133 172 120 162 209 114 149 183 134 176 224 130 192 236 124 165 207 147 195 249 144 189 230 134 187 235 123 162 188 102 140 182 117 154 192 151 189 235 105 145 191 124 169 220 151 203 271 Mean 57 79 127 170 215 Compound Administration Weeks 67 201 228 269 297 295 314 251 265 210 234 266 305 208 234 270 291 276 300 239 272 293 321 238 299 258 297 221 250 221 258 219 268 285 328 225 249 229 289 285 324 248 281 Sacrificed 325 351 371 412 Sacrificed 268 292 342 365 Sacrificed 325 356 321 334 297 328 355 326 327 355 317 348 281 306 280 300 306 339 359 385 274 303 324 352 377 408 321 345 * Dosage level increased in the 5th week of study to 1000 ppm. 8 374 443 320 386 381 359 357 391 379 381 329 328 369 412 330 372 436 373 9 10 387 410 465 485 Sacrificed 407 430 378 415 379 397 Sacrificed 399 445 Sacrificed 396 423 345 368 334 351 365 390 425 453 346 372 385 398 446 467 390 415 Sanitized. Does not contain t s c a c b i Company h3 0(0I TABLE 6. Rat Number Ninety-Bay Feeding Study in the Rat. Continued. Individual Weekly Body Weights, Grams. 500 ppm. ~ Male: 14243 14244 14247 14249 14250 14252 14254 14255 14256 14257 14258 14259 14260 14261 14321 14322 14325 445 435 422 509 525 499 449 471 447 434 451 441 412 431 411 453 463 434 452 437 428 382 396 380 369 377 348 408 415 390 478 384 400 500 499 404 417 520 477 419 434 548 517 435 452 568 496 446 458 542 545 465 476 563 507 487 532 516 497 531 549 525 562 Mean 434 446 434 506 493 525 Initiation of withdrawal period (14th week). S a c r ific e d S a c r ific e d S a c r ific e d 582 612 538 559 545 550 569 586 559 577 632 544 549 577 576 Company Sanitized. Does not contain TSCACBI Ninety-Day Feeding Study in the Rat. TABLE 7. Individual Weekly Body Weights, Grams. kat Number Control Period 12 ___________________ 12 3 * 2500 ppm . - Female 14262 14263 14264 14265 14266 14267 14268 14269 14270 14271 14272 14273 14274 14275 14276 14277 14278 14279 14280 14281 52 53 53 55 50 55 63 56 56 60 58 51 47 57 52 52 47 61 56 53 87 79 71 72 63 76 85 86 85 95 74 66 61 75 63 66 55 76 75 66 120 119 107 104 78 113 114 115 116 134 117 116 99 122 107 106 85 123 126 108 138 145 124 127 109 134 132 130 136 161 134 140 133 144 135 134 115 144 154 132 164 166 140 143 133, 147 137 148 151 180 148 161 151 168 148 152 141 160 178 146 Mean 55 75 113 136 154 Compound Administration. Weeks 4567 174 182 149 154 153 163 135 163 161 187 158 169 171 174 162 151 149 155 173 142 161 181* 200 160 165 171 175 158 173 171 212 173 185 182 198 179 168 174 185 204 171 179 Sacrificed 230 246 169 180 Sacrificed 181 195 188 202 Sacrificed 182 192 174 184 225 240 186 193 197 202 195 206 207 222 191 209 188 196 189 198 206 211 221 233 180 189 196 207 * Dosage level increased in the 5th week of study to 5000 ppm. 8 264 195 208 216 200 193 246 200 210 219 233 226 208 216 222 244 193 218 9 10 Sacrificed 194 207 222 228 . 223 232 202 216 Sacrificed 256 260 207 213 220 226 215 225 Sacrificed 231 243 205 223 221 230 230 236 256 265 207 207 221 229 Company Sanitized. Does not c o p ^ n T S C A C J I N5 G> 14264 14266 14267 14269 14271 14272 14273 14274 14276 14277 14278 14279 14280 14281 14311 14326 14329 Mean* 205 216 201 226 228 213 234 244 213 214 217 208 261 264 244 207 210 204 226 233 223 226 218 212 262 260 263 228 232 212 229 236 274 214 241 239 276 216 231 237 276 218 249 257 278 222 259 254 284 239 270 269 285 245 Sacrificed Sacrificed Sacrificed 252 258 257 233 246 253 260 278 278 233 236 239 246 258 261 198 202 209 207 220 218 232 235 225 239 246 253 241 254 254 * Initiation of withdrawal period. (14th week) Page 27 Company Sanitized. Does not contain TSCA CBI Ninety-Day Feeding Study in the Rat. TABLE 8. Individual Weekly Body Weights, Grams. Rat Number Control Period 12 1 2 3 4 tia ulull WfcifciK.0 567 8 9 10 * 2500 ppm. - lale 14282 14283 14284 14285 14286 14287 14288 14289 14290 14291 14292 14293 14294 14295 14296 14297 14298 14299 14300 14301 60 80 53 84 55 76 55 84 52 65 55 84 46 71 60 98 56 84 57 60 52 69 57 82 58 82 64 92 61 84 58 75 52 82 63 89 55 78 52 76 117 141 184 225 246 Sacrificed 124 151 193 237 268 299 321 343 354 374 129 182 219 277 310 351 382 416 440 454 133 178 226 260 283 Sacrificed 92 125 147 173 203 222 245 270 291 302 133 181 227 262 289 308 332 357 371 388 193 171 227 265 274 Sacrificed 151 194 249 298 322 373 404 437 Sacrificed 125 154 193 234 275 314 335 366 387 399 107 163 206 228 271 306 332 354 370 381 72 104 138 181 203 239 273 297 315 334 143 184 228 261 285 312 339 364 Sacrificed 133 165 207 231 266 291 315 337 339 355 149 189 228 265 294 341 378 416 Sacrificed 144 189 .231. . 272 295 321 345 363 365 385 133 196 247 281 323 363 396 385 422 446 107 140 178 214 236 258 284 306 312 331 143 190 241 290 325 351 378 406 411 435 114 135 197 215 272 303 325 342 345 370 132 169 205 240 271 292 312 332 345 370 Mean 56 80 129 165 209 245 276 308 335 358 362 380 increased in the 5th week of study to 5000 ppm. Company Sanitized. Does not contain TSCA CBt Ninety-Day Feeding Study in the Rat. TABLE 8. Continued. Individual Weekly Body Weights, Grams. Rat Number 11 12 13 Compound Administration Weeks 14* 15 16 17 18 25U DDTTl. - Male : 14283 14284 14286 14287 14290 14291 14292 14294 14296 14297 14298 14299 14300 14301 14327 14328 14324 Mean 394 410 393 478 507 497 318 328 316 400 414 401 415 425 413 401 410 385 344 373 357 367 388 364 405 418 412 468 480 456 349 465 327 341 339 342 Sacrificed 453 381 382 463 395 398 476 407 414 472 416 431 473 429 447 512 442 448 Sacrificed Sacrificed 466 486 362 377 390 497 427 492 500 517 537 550 461 478 489 500 516 397 420 401 425 435 449 500 495 * Initiation of withdrawal period. (14th week) 19 508 433 556 531 507 Page 29 Company Sanitized. Does not contain TSCACB3 FIGURE 1. Group Mean Body Weights. Female Rats 500 S3 Control 450 O 100 ppm. 400 350 -- A 500 ppm. - 1000 ppm. o 2500 ppm. - 5000 ppm. 300 250 200 - 150 - 100 _ 50 ( < o oA A fi A o' c 0 1 ___ 1 00 1 1 ___ 1_ 23 1 4 J------- J --------- 1--------- 1______ I______ 1 I I I I I______ L J 7 8 9 10 11 12 13 14 15 16 17 18 u COMPOUND ADMINISTRATION WEEKS Company Sanitized. Does not contain TSCA CBi ^I OQ > fl) FIGURE 2. Group Mean Body Weights. Male Rats Company Sanitized. Does not contain TSCA CBI A C __ I 19 Ninety-Day Feeding Study in the Rat. LE 9. Mean Body Weights, Grams; Weight Ranges, Grams; and Survival: impound Sminisation feeks Mean Body Wt. Control Weight Ranges Survival Mean Body Wt. 100 ppm. Weight Ranges Survival Mean Body Wt. FEMALE RATS 500 ppm.* Weight Ranges Survival Mean Body Wt. ** Weight Ranges Survival 0 55 50-60 20/20 56 51-62 20/20 55 48-60 20/20 55 47-63 20/20 0 75 48-101 20/20 74 58-101 20/20 75 52-92 20/20 75 55-95 20/20 1 112 82-136 20/20 112 .91-144 20/20 113 86-130 20/20 113 78-134 20/20 2 138 117-163 20/20 137 119-176 20/20 139 115-157 20/20 136 109-161 20/20 3 157 134-187 20/20 158 139-210 20/20 157 130-184 20/20 154 133-180 20/20 4 169 143-207 20/20 177 144-252 20/20 172 140-201 20/20 161 135-187 20/20 5 188 153-249 17/20 191 174-251 17/20 188 154-219 17/20 179 160-212 17/20 6 203 166-260 17/20 212 185-275 17/20 204 170-237 17/20 196 169-230 17/20 7 234 165-285 17/20 227 193-290 17/20 216 181-254 17/20 207 180-246 17/20 8 229 174-296 17/20 242 207-303 17/20 229 191-272 17/20 218 193-264 17/20 9 236 178-306 14/20 251 215-307 14/20 243 201-287 14/20 221 194-256 14/20 10 251 135-314 14/20 269 234-331 14/20 255 204-303 14/20 229 207-265 14/20 11 260 230-320 14/20 277 236-345 14/20 257 212-310 14/20 232 207-274 14/20 12 266 230-326 14/20 285 248-353 14/20 268 216-314 14/20 235 210-276 14/20 13 258 191-332 13/20 268 221-333 14/20 254 207-299 14/20 225 201-276 14/20 14 267 250-289 3/20 0/20 259 249-274 6/22 248 222-278 5/22 15 277 254-299 3/20 270 258-278 6/22 256 239-284 5/22 16 284 256-310 3/20 273 266-280 6/22 264 253-285 5/22 17 0/20 269 265-272 3/22 256 252-260 2/22 18 280 273-284 3/22 268 258-278 2/22 19 281 276-284 3/22 243 228-257 2/22 awai period was initiated following 13-weeks of compound administration. Selected animals were continued into the Perid o_ withdrawal which continues at the writing of this report. f'Dosage level in this group increased to 1000 ppm. in the 5th week of studgompany Sanitized. Does not contain TSCA CB1 aiot) Jj^osage level in this group increased to 5000 ppm, ih the 5th week of study, LO N iinety-Day Feeding Study in the Rat. lBLE impound Iminisation ieeks Mean Body Wt. Control Weight Ranges Survival s, Grams ; Weight Ranges, Grams ; and Survival: MALE RATS. Mean Body Wt. 100 DDI. Weight Ranges Survival Mean Body Wt. 500 DDm * Weight Ranges Survival 0 55 45-64 20/20 57 49-63 20/20 58 52-63 0 79 55-100 20/20 79 58-103 20/20 80 63-98 1 128 89-157 20/20 127 97-160 20/20 127 91-151 2 175 143-214 20/20 168 122-215 20/20 169 125-203 3 224 187-263 20/20 214 130-267 20/20 215 172-271 4 261 231-309 20/20 252 168-310 20/20 248 201-293 5 287 250-346 17/20 277 211-343 17/20 281 234-324 6 318 283-366 17/20 308 250-371 17/20 321 268-377 7 344 306-388 17/20 335 256-407 17/20 345 292-412 8 376 308-446 17/20 362 296-448 17/20 373 320-443 9 397 341-461 14/20 376 317-467 14/20 390 334-465 10 420 358-480 14/20 409 300-495 14/20 415 351-485 11 434 365-505 14/20 429 353-521 13/20 434 369-509 12 444 374-505 14/20 446 368-527 13/20 446 377-525 13 429 357-506 14/20 437 363-515 13/20 434 348-548 14 447 242-538 7/23 0/20 472- 234-568 15 443 249-518 7/23 461 236-542 16 465 251-556 7/23 489 241-563 17 469 256-574 4/23 496 246-582 18 471 255-571 4/23 511 250-612 19 480 254-585 4/23 511 253-632 TE - W,,ith.drjawa-l `period wa,,s -in-i-t"iatwwev* i.uii.uwiuS j.j-wt;eK.5s ox compound administrati period of withdrawal which continues at the writing of this report. ** nno!gS i`eVei ^ -3 s*oup lncreased to 1000 ppm. in the 5th week of study. '^ 86 level ln thls SrouP increased to 5000 ppm. in the 5th week of study. 20/20 20/20 20/20 20/20 20/20 20/20 17/20 17/20 17/20 17/20 14/20 14/20 14/20 14/20 14/20 8/24 8/24 8/24 5/24 5/24 5/24 Mean Body Wt. 56 80 129 165 209 245 276 308 335 358 362 380 397 420 401 378 387 399 394 410 418 .... 1 Weight Ranges Survival 45-64 60-98 72-193 104-196 138-249 173-298 203-325 222-373 245-404 270-437 291-440 302-454 318-478 328-507 316-497 198-492 202-500 209-517 207-537 220-550 218-556 20/20 20/20 20/20 20/20 20/20 20/20 17/20 17/20 17/20 17/20 14/20 14/20 14/20 14/20 14/20 9/25 9/25 9/25 6/25 6/25 6/25 into th 0> o(oD Sam1i2ea- D* - contain T S C A C B , TABLE 10. Ninety-Day Feeding Study in the Rat. FEMALE RATS: Mean Food Consumption, Grams/Rat/Week and Grams/Kilogram/Day; Miligrams/Kiloerams/Dav and Fnnd Compound Consumption as H uunsumpcion as Compound CONTROL tration FOOD week g/r/wk g/kg/d 0 1 96.1 122.3 2 110.5 114.5 3 110.8 100.6 4 104.4 88.1 5 113.8 86.7 6 105.7 74.4 7 111.5 67.9 8 113.2 70.7 9 108.1 65.3 10 102.4 58.2 11 112.6 61.6 12 116.2 62.4 13 123.9 68.6 14 ***5 8 .0 54.3 15 110.7 57.0 16 114.0 57.4 17 18 19 Eff. 0-24 0.17 0.11 0.17 0.14 0.28 -.04 0.07 0.15 0.09 0.05 -.05 + .09 + .09 + .06 100 ppm. FOOD g/r/wk g/kg/d 99..6 110..7 116..6 113,.7 113,.1 110,.8 115..4 117,.7 112,.8 121,.4 129..4 124..0 128..1 126.8 115.3 105.7 91.5 84.8 74.5 72.7 69.4 64.1 64.3 66.8 62.1 68.3 Eff. 0. 23 0. 18 0. 17 0. 12 0. 13 0. 13 0. 13 0. 08 0. 15 0.06 0. 06 13 CPD, 500 ppm. * FOOD mg/kg/d g/r/wk g/kg/d Eff. 12..7 11,.5 10..6 9..2 8..5 7..5 7..3 6..9 6..4 6..4 6..7 6..2 6,.8 .98.9 111.9 117.9 108.1 110.2 105.5 110.5 111.7 112.2 123.9 116.7 116.8 128.8 ***59.7 113.7 111.0 109.3 111.0 110.7 124.8 115.1 107.0 89.5 83.5 74.0 73.1 69.9 65.8 69.4 65.0 62.3 72.4 57.5 60.0 58.2 58.0 56.8 56.2 0.23 0.15 0.14 0.15 0.15 0.11 0.12 0.12 0.10 0.02 0.09 -.11 +.05 + .10 + .03 -.04 + .01 +.01 CPD, 2500 ppm. ** FOOD mg/kg/d g/r/wk g/kg/d Eff. 62.4 57.6 53.5 44.8 41.8 74.0 73.1 69.9 65.8 55.9 65.0 62.3 72.4 91.4 107.0 108.9 95.4 107.6 100.2 102.7 103.5 93.6 107.8 98.0 97.2 112.0 ***66.8 118.0 115.2 121.0 119.5 111.5 115.9 112.5 101.3 84.5 86.0 73.0 71.0 67.9 60.6 67.2 60.3 59.1 71.1 63.7 66.0 61.4 67.6 63.8 65.4 0.21 0.17 0.07 0.17 0.17 0.11 0.10 0.03 0.07 0.03 0.03 -.09 + .20 + .07 + .07 -.07 + .10 -.22 CPD. mg/kg/d 289.8 304.0 253.3 211.3 215.0 365.0 355.0 339.5 303.0 336.0 301.5 295.5 355.5 , - Withdrawal period was initiated following-13-weeks of compound administration, Selected animals were continued into the period of withdrawal which continues at the writing'of this report. * Dosage level increased to 1000 ppm. in the 5th week of study. ** Dosage level increased to 5000 ppm. in the 5th week of study. ***' Grams/rat/4 days. hd ( 0(QD Does no! contain TSCA CBf N5 Compound Adminis tration CONTROL . ______ FOOD________ week g/r/wk g/kg/d Eff. 0 1 108.8 121,.1 2 135.5 110,.9 3 150.3 96,,0 4 151.7 83..1 5 6 151.0 146.6 75,,3 0. 11 65,,7 0. 21 7 . 155.1 64,.5 0. 17 8 165.2 62,.8 0. 19 9 154.1 55,,4 0. 14 10 159.9 54.,3 0. 14 11 158.1 52,,1 0.09 12 162.8 52,.5 o. 06 13 184.8 61,.5 08 14 ***80 -.4 45.,0 +. 13 15 145.1 46,,7 03 16 159.0 48,.8 + .14 17 162.0 49,.3 + .02 18 149.3 45,.2 + .01 19 149.5 44,,6 +. 06 ------IQOi-PPtn. ________ _ ______500 ppm.* ______ FOOD_______ CPD. _____ FOOD _____ g/r/wk g/kg/d Eff. mg/kg/d g/r/wk g/kg/d Eff. 99.6 126.3 141.9 151.1 152.8 148.0 157.1 158.2 154.1 166.6 175.2 170.9 186.2 118.3 107.1 94.9 85.7 78.7 68.5 66.9 62.4 58.5 58.2 58.3 54.7 60.9 0.33 0.32 0.25 0.20 0.21 0.18 0.17 0.09 0.20 0.11 0.10 -.07 11.8 10.7 9.5 8.6 7.9 6.9 6.7 6.2 5.9 5.8 5.8 5.5 6.1 ' 100.4 128.8 144.9 145.3 154.9 150.0 151.6 161.8 148.6 162.5 164.4 159.6 181.3 ***89.3 150.3 169.0 167.4 145.0 161.8 112.6 108.9 96.3 83.9 78.6 66.7 62.9 61.9 54.4 55.9 54.1 51.1 59.7 47.2 46.6 49.3 48.2 40.5 45.2 0.33 0.31 0.23 0.21 0.26 0.16 0.17 0.11 0.15 0.12 0.08 -.07 + .24 -.07 + .17 + .04 +.10 0 _______ _ _____ 2500 ppm. ** CPD. ______ FOOD______ _ mg/kg/d g/r/wk g/kg/d Eff. 56.3 54.5 48.2 42.0 39.3 66.7 62.9 61.9 54.4 55.9 54.1 51.1 59.7 112.2 121.6 140.5 145.0 151.9 149.9 152.3 154.1 140.7 146.8 156.4 150.6 178.5 ***79.6 138.8 138.4 138.3 143.5 145.5 124.0 10-5.5 95.7 84.5 78.6 69. 65.1 61.5 55.5 38.7 56.2 51.2 63.6 52.6 51.2 49.6 50.3 50.0 49.8 0.30 0.31 0.25 0.20 0.21 0.18 0.15 0.03 0.12 0.03 0.03 -.11 -.17 -.06 + .09 -.04 + .11 + .05 CPD. mg/kg/d 310.0 263.8 239.3 211.3 196.5 347.5 325.5 307.5 277.5 293.5 281.0 256.0 318.0 NOTE - Withdrawal period was initiated following 13-Weeks of compound administration into the period of withdrawal which continues at the writing of this report. * Dosage level increased to 1000 ppm. in the 5th week of study. ** Dosage' level increased to 5000 ppm. in the 5th week of study. *** Grams/rat/4 days. Company Sanitized. Does not contain TSCA CBi| Selected animals were continued *oT>} 0f9l> WU> > Ninety-Day Feeding Study in the Rat. TABLE 12. Summary of Hematologic Values for Male Rats. Hematology Compound Adminis tration Month Control 100 ppm. Page 34 500 ppm. * 2500 ppm. * * Hematocrit, % Hemoglobin gms./100 ml. Erythrocytes, xlO /cmm. Leucocytes, xlO3/CT1T1. 0 47 45 45 44 1 48 46 47 43 2 48 45 45 38 3 48 47 45 38 0 12.7 12.7 12.3 12.4 1 14.6 14.1 14.5 13.3 2 15.9 15.2 15.3 13.2 3 15.7 15.0 14.7 12.0 0 6.38 6.04 6.21 6.04 1 6.60 6.74 6.43 6.31 2 '7.21 7.55 7.35 6.29 3 7.16 7.05 7.06 5.90 0 10 75 9.90 12.48 11.30 1 11.79 11.21 8.83 8.62 2 11.22 9.40 11.24 11.93 3 15.25 10.28 11.15 11.55 Comnany Sanitized. Does not contain T S C A CB! fa Neutrophils, 7 Seg., T, Non-Seg., 7 Lymphocytes, % Monocytes, % Eosinophils, 7 Basophils, T. 0 15 . 15 15 12 1 7 17 14 11 2 14 - 13 14 11 3 17 20 16 8 0 11 0 1 1 00 0 0 2 00 0 0 3 00 0 0 0 83 82 83 85 1 90 82 85 87 2 83 84 84 87 3 80 78 82 89 0 11 1 1 1 20 1 1 2 32 1 1 3 20 1 1 0 01 1 1 1 11 0 1 2 01 1 1 3 12 1 2 0 00 0 0 1 00 0 0 2 00 0 0 3 00 0 0 )up increased to 1000 ppm. in the 5th week of study. )up increased to 5000 ppm. in the 5th week of Study. Ninety-Day Feeding Study in the Rat. TABLE 13. Summary of Hematologic Values for Female Rats. Hematology Compound Adminis tration Month Control 100 ppm. Page 35 500 ppm.* 2500 ppm, Hematocrit, % 0 , 49 45 46 46 1 49 46 45 43 2 46 44 41 33 3 45 47 40 33 Hemoglobin gms./lOO ml. 0 13.3 12.3 12.4 12.9 1 15.1 14.4 14.4 13.5 2 -15.8 14.8 14.3 11.7 3 15.6 14.9 13.6 10.6 Erythrocytes, xlO^/cmm. 0 6.63 6.38 6.23 6.51 1 7.13 6.82 6.51 6.30 2 6.74 6.81 6.57 5.44 3 6.64 6.82 6.21 5.54 Leucocytes, xlO'Y cmm. 0 12.64 13.72 11.73 11.72 1 9.41 .7.40 9.08 11.53 2 11.76 9.27 8.76 8.67 3 10.96 9.04 10 il4 11.38 Neutrophils, % Seg., % Non-Seg., % . 0 14 11 14 11 1 15 17 14 15 2 15 13 17 12 3 11 20 11 8 0 00 0 0 1 00 0 0 2 00 0 0 3 10 0 0 Lymphocytes, "L 0 83 87 84 88 1 81 81 84 84 2 '82 83 79 87 3 85 77 88 90 Monocytes, % 0 12 1 1 1 21 1 1 2 22 2 1 3 22 0 1 Eosinophils, 7 0 10 1 0 1 21 1 0 2 12 2 0 3 11 1 1 Basophils, 7= 0 00 0 0 1 00 0 0 2 00 0 0 3 00 0 0 * Dosage level in this group increased to 1000 ppm. in the 5th week of study. ** Dosage level in this group increased to 5000 ppm. in the 5th week of study. ,mpany Sanif2ed- Does no! contain TS CA CBi Ninety-Day Feeding Study in the Rat, TABLE 14. Rat ^dividual Rat Hematologic Values during Control Period. No. & Hematocrit Hemoglobin Erythrocvtes t Ttal ------- - -- ex ______ Y______ gms./lQQ ml. (xl06/cmm.) ^ " `Z - S a g . z Control: ------ --- -- -- ------ ----- Z-------- 14163M 14167M 14173M 14175M 14178M 14181M Mean 14143F 14146F 14150F 14154F 14157F 14160F Mean 100 ppm. 46 50 45 46 48 45 47 48 48 48 50 49 49 49 12.6 14.0 12.3 12.7 12.6 12.0 12.7 13.2 12.5 13.2 13.9 13.1 13.6 13.3 6.09 6.90 6.42 6.01 6.94 5.91 6.38 6.14 6.14 6.89 6.98 6.40 7.22 6.63 7.29 14.00 12.82 10.32 11.52 8.57 10.75 16.18 12.34 14.18 13.03 9.74 10.38 12.64 7 13 31 9 21 9 15 9 19 20 8 7 21 14 1 0 0 2 1 0 1 0 0 0 0 0 0 0 92 84 69 85 78 91 83 90 80 75 91 92 78 84 14204M 14208M 14210M 14205M 14216M 14220M Mean 14184F 14189F 14191F 14194F 14198F 1420 If Mean 49 43 44 43 45 46 45 46 41 45 46 45 49 45 13.4 13.4 12.1 12.2 12.0 12.9 12.7 12.1 11.1 12.0 12.6 11.7 14.1 12.3 6.50 5.40 6.20 5.81 6.10 6.23 6.04 6.02 5.55 6.07 6.25 6.02 8.36 6.38 9.65 8.63 12.96 7.36 6.44 14.36 9.90 25.67 14.31 10.55 10.63 9.78 11.40 13.72 16 15 16 19 13 8 15 15 14 6 5 11 14 11 0 0 2 1 0 0 1 0 0 0 0 0 0 0 78 84 81 78 87 90 82 81 84 92 95 85 84 87 'bompany Sanitized. Does not contain T S C A B Company San^zcd. l o . . z c : ia t *osinc,pillla Basoph L% % 000 300 000 400 000 000 10 0 10 0 10 0 320 100 010 0 10 110 420 10 0 0 10 110 00 0 110 110 400 110 200 000 310 20 0 26 0 TTpnO>>3 LO CTv Ninety-Day Feeding Study in the Rat 500 ppm 14244M 14247M 14249M 14251M 14255M 14257M Mean 14225F 14227F 1423IF 14233F 14236F 14241F Mean 2500 ppm.: 14284M 14288M 14290M 14295M 14298M 14301M Mean 14264F 14267F 14269F 14276F 14278F 14281F Mean 44 46 44 43 42 44 44 46 46 45 51 43 45 46 12.5 12.9 12.4 12.3 12.0 12.0 12.4 13.0 12.9 12.2 13.7 12.2 13.4 12.9 6.03 6.48 6.01 56..2722 5.77 6.04 6.25 6.58 6.13 6.76 7.16 6.20 6.51 15.14 10.32 13.88 8.92 10.30 9.21 11.30 11.62 11.10 8.96 11.35 17.47 9.84 11.72 18 12 10 11 13 8 12 13 7 11 9 8 18 11 1 0 1 0 1 0 1 1 0 0 1 0 0 0 79 88 87 86 85 90 85 85 91 87 89 90 81 88 2 0 1 2 0 2 1 1 2 1 0 2 1 1 0 0 1 1 1 0 1 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Company Sanitized. Does not contain TSCA CBI in n> to Ninety-Day Feeding Study in the Rat. TABLE 15. Individual Rat Hematologic Values at One Mpnth. Rat No. & Hematocrit Sex % Control: Hemoglobin gms./lOO ml Total Erythrocytes Leucocytes Neutrophils (xlO /cmm.) (xl03/cmm.) Seg.% Non-Seg.% Differential LympHocytes Monocytes Eosinophils Basophil^ 7 % % 14162M I4165M 14168M 1417LM 14174M 14177M Mean 14142F 14144F 14146F 14148F 14150F 14153F Mean 50 46 47 47 48 47 48 48 48 50 51 47 49 49 15.4 13.9 14.8 14.5 14.4 14.5 14.6 7.18 6.66 6.80 6.22 6.44 6.30 6.60 14.40 14.75 9.27 14.34 10.88 7.08 11.79 8 6 6 5 7 10 7 0 0 0 0 0 0 0 86 91 90 94 92 88 90 5 3 3 1 1 1 2 14.7 15.2 15.5 15.2 14.3 15.4 15.1 6.87 7.04 8.12 7.27 6.61 6.89 7.13 7 .82 8..80 11,.60 10,,53 7,,00 10,,72 9.,41 14 19 18 17 14 9 15 0 0 0 0 0 0 0 82 77 77 80 80 89 81 1 1 3 1 3 1 2 1 0 1 0 0 1 1 3 3 2 1 3 1 2 0 0 0 0 0 0 0 0 0 0 1 0 0 0 100 ppm.: 14202M 14205M 14208M 14211M 14214M 14217M Mean 14182F 14185F 14188F 14191F 14194F 14197F Mean 44 48 47 45 49 45 46 47 44 46 48 45 46 46 13.6 14.8 14.6 13.7 13.8 14.1 14.1 6.32 6.73 7.08 6.54 7.03 6.72 6.74 19.70 7.10 8.22 6.78 11.69 13.77 11.21 7 25 30 9 23 7 17 0 0 0 0 0 0 0 90 74 69 88 76 92 82 1 o 0 l o l o 14.2 13.6 14.3 14.8 13.9 14.4 14.4 6.69 6.68 6.91 7.10 7.28 6.30 6.82 9.15 3.97 8.64 6.98 6.53 9.15 7.40 6 46 11 18 11 8 17 0 91 3 49 0 87 0 81 0 86 0 91 0 81 1 l 0 1 2 1 1 2 1 1 2 1 0 1 2 1 2 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Company Sanitized. Do< not contain T S C A CB1 'MT) O(PD to f) NinetyDay Feeding Study in the Rat, TBLt Rat No, Sex Continued. Individual Rat Hematologic Value s at One Month, Hematocrit % u , .. Total Hemoglobin Erythrocytes Leucocytes gtBS,/100 m ! , (xioe/c^.) (*l03/L..) Neutroohois 14242M 14245M 14248M 14251M 14254m .4257M Mean 14222F 1422.5F 14228F 14231F 14234F 14237F Mean 51 45 47 46 47 48 47 45 46 45 45 46 45 45 2500 ODm.: 14282M 14285M 14288M 14291M 14294M 14297M Mean 14262F 14265F 14268F 14271F 14274F 14277F Mean 42 44 42 45 41 45 43 44 42 46 41 40 44 43 15.9 13.6 14.6 13.9 14.7 14.5 14.5 14.4 14.3 14.4 14.2 14.4 14.5 14.4 13.2 13.9 12.6 13.7 12.5 13.9 13.3 13.6 13.3 14.6 13.0 12.9 13.8 13.5 7.07 6.03 6.77 6.17 5.99 6.55 6.43 6.55 6.83 6.20 6.42 6.69 6.35 6.51 6.02 6.57 5.93 6.68 6.14 6.50 6.31 6.12 6.07 7.19 5.96 5.95 6.53 6.30 7.48 7.84 9.75 11.62 8.16 8.13 8.83 11.49 9.65 7.68 9.95 5.79 9.90 9.08 17 14 9 12 10 ' 20 14 8 10 11 21 20 14 14 9.79 11.49 8.82 6.71 6.54 8.34 8.62 19.29 9.08 6.28 13.43 8.84 12.23 11.53 8 ii 9 18 11 10 11 9 16 14 18 16 16 15 0 1 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 Differential Lymphocytes Monocytes Eosinoph 1 % 82 0 84 1 90 1 87 1 90 0 79 0 85 1 90 2 89 1 87 0 77 1. 79 1 83 1 84 1 1 0 0 0 0 1 0 0 0 2 0 0 2 1 91 0 88 1 90 1 82 0 85 3 89 0 87 1 1 0 0 0 1 1 1 88 3 0 83 1 - 0 85 0 1 80 1 1 83 1 0 81 2' 0 84 1 0 C o m p ly Doss not contain T S C A CB| T 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Page 3 TABLE 16 Individual Rat Hematologic Values at Two Months Rat No. & bex Hematocrit /,, Hemoglobin Erythrocytes gnmnsn .//1l5A r5t mH. " .(' xn fK fi j w ). Total Leucocytes ( Qo. VJ c ^ O Control : 14164m 14167 m 14170M 14174M 14178M 14181M Mean 14I43F 14147F 14149F 14152F 14154F 14158F Mean 47 49 52 47 47 48 48 47 48 49 41 50 42 46 100 pom.: 14204M 14210M 14213M 14217M 14220M 14221M Mean 50 44 41 42 50 40 45 14184F 14190F 14193F 14194F 14196F 14200F Mean 45 45 41 44 43 43 44 15.4 1^.5 16.6 15.6 16.0 15.4 15.9 15.4 16.2 16.4 15.2 15.8 16.0 15.8 16.0 14.9 14.8 14.9 15.8 14.5 15.2 15.2 15.0 13.6 15.1 15.1 14.5 14.8 7.02 7.43 7.47 7.07 7.00 7.24 7,21 6.67 6.78 7.26 6.36 7.03 6.31 6.74 7 .84 7 .55 7 .87 6 .84 8,.02 7,.20 7,.55 6,.85 6,,74 6.,12 6.,71 6..98 7. 47 6. 81 11.44 13.95 11.11 9.73 11.22 9.88 11.22 11.86 15.15 10.64 10.92 12.83 9.16 11.76 10 .75 9 .29 12 .15 7 .30 9,.37 7,.56 9,.40 10..00 6..42 7,,11 6,,23 13.,75 12. 12 9. 27 7 12 5 31 19 8 14 32 9 9 22 7 8 15 19 8 14 8 15 12 13 7 22 10 12 14 10 13 ^ m o a n y Sanitized. Poes no! eonfain T S G A GBI Differential Lymphocytes Monocytes Eosinophils Basophils 0 90 2 0 83 5 0 93 1 1 0 1 0 0 0 0 64 5 0 0 0 81 0 0 90 2 0 83 3 0 0 0 0 0 0 0 67 1 0 0 0 87 3 1 0 0 88 1 2 0 0 78 0 0 90 3 0 0 0 0 0 90 2 0 82 2 0 1 0 0 0 75 3 3 0 0 90 1 1 0 0 84 1 0 91 1 1 0 0 0 0 82 2 1 0 0 85 3 0 84 2 0 1 0 0 0 90 1 2 0 2 69 4 0 87 2 0 83 3 0 85 0 0 90 0 0 83 2 3 1 2 1 0 2 0 0 0 0 0 0 t; cu 0rQd Ninety-Day Feeding Study in the Rat. TABLE 16. Continued. Individual Rat Hematologic Values at Two Months. Rat No. 6 Hematocrit oex % 1000 ppm. Hemoglobin Erythrocytes gms./100 ml. (xl06/cmm.) Total ---------------------- Differential Leucocytes (xlC)3/cmm, ) -- Neutrophils Seg.% Non-Seg.% Lymphocytes Monocytes %y Eosinophils Basophils % ?/o 14243M .14246M 14251M 14253M 14257M 14261M Mean 14223F 14226F 14230F 14234F 1423-7F 14240F Mean 42 46 47 46 46 42 45 42 43 41 37 43 38 41 5000 ppm. 15.4 15.2 15.4 14.8 15.9 15.1 15.3 7.71 7.11 7.42 6.94 8.03 6.89 7.35 9.97 12.04 12.47 8.86 10.67. 13.43 11.24 23 13 11 15 6 13 14 0 0 0 1 0 1 0 75 84 87 82 93 82 84 1 1 l l o 2 l 15.5 14.2 14.3 13.6 15.8 12.4 14.3 7.28 6.63 6.28 6.63 6.85 5.77 6.57 8.34 6.64 10.28 6.17 13.43 7.68 8.76 16 18 8 24 28 6 17 1 0 0 0 0 0 0 81 72 92 74 62 94 79 o 5 0 1 8 0 2 1 2 1 1 1 2 1 2 5 0 1 2 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 14283M 14289M 14293M 14295M 14297M 14300M Mean 14263F 14270F 14273F 14275F 14279F 14281F Mean 42 34 35 36 38 44 38 37 29 35 33 30 33 33 13.3 12.0 12.3 12.7 13.6 15.0 13.2 6.33 6.12 6.09 6.13 6.08 6.96 6.29 11.96 17.50 11.20 6.97 14.49 9.46 11.93 7 23 17 8 4 9 11 0 0 0 0 0 1 0 92 0 76 1 81 1 91 * 0 94 1 87 3 87 1 13.0 11.2 12.1 11.8 10.4 11.7 11.7 5.60 5.31 5.64 5.47 4.93 5.68 5.44 11.57 5.16 9.55 8.90 6.78 10.07 8.67 10 14 8 8 17 17 12 1 87 0 84 0 91 0 92 0 81 0 82 0 87 2 2 1 o 1 o 1 1 0 1 1 1 0 1 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Company Sanitized. Does not contain TSCA CB S PS TCOD Ninety-Pay Feeding Study in the Rat. TABLE 17. Individual Rat Hematologic Values at Three Months. Rat No.6, He,toctit Control : i JJmoglobtn Erythrocytes L e o c o c y L gms./OOml. "ciloE^cTM" (xl03/cTM!> ~-- -- ------ 14164M 14169M 14172M 14176M 14179M 14181M Mean 14147F 14151F 14154F 14156F 14158F 14161F Mean 53 46 47 48 47 50 48 46 43 45 41 47 45 45 100 ppm.: 16.1 16.0 15.6 15.6 15.2 15.6 15.7 16.9 15.1 15.2 15.1 16.0 15.2 15.6 7 .52 6 .68 7 .14 6..79 7,.53 7,.10 7,.16 6..64 6..52 6.,66 6. 33 7. 02 6. 69 6. 64 17 .93 21 .59 14,,62 13,.86 10,.06 13,.45 15,.25 13..71 7.,16 12.,03 7. 12 11. 53 14. 18 10. 96 15 6 23 15 17 24 17 7 18 15 9 5 13 11 14203M 14206M 14210M 14215M 14218M 14219M Mean 14183F 14186F 14190F 14195F 14197F 14200F Mean 45 50 47 46 46 50 47 49 50 38 49 50 46 47 14.5 15.2 14.8 15.1 14.6 15.6 15.0 15.6 15.3 12.0 15.9 16.0 14.8 14.9 6.60 7.47 6.91 6.80 6.90 7-.60 7.05 6.89 7.26 6.09 6.89 6.77 7.03 6.82 13.85 10.73 9.58 7.47 12.35 7.71 10.28 12.16 10.83 6.65 5.57 11.79 7.25 9.04 40 24 7 20 16 12 20 13 29 28 ii 15 26 20 Company Sanitized. Does not contain TSCA CBS <9 Differential Eycphocytes Monocytes Eosinophils Basophils' s' /o to % yo 0 82 3 0 94 0 0 70 5 0 0 0 0 84 1 '0 0 80 1 9 0 74 2 i 0 80 2 l 1 89 2 2 80 0 1 79 1 1 88 1 0 91 2 0 84 2 1 85 1 0 4 1 9 1 2 1 58 1 0 73 0 0 91 0 1 78 0 0 82 0 0 87 0 0 78 0 0 85 2 0 69 1 3 63 3 0 87 1 0 85 0 0 73 1 0 77 2 n 3 2 1 2 1 2 0 n 2 1 0 0 1 o 0 u 0 0 0 0 0 0 0 0 0 0 0 0 o 0 u u 0 n 1 1 0 0 0 0 0f0lQ)> 4r>o Ninety-Day Feeding Study in the Rat. TABLE 17. Continued. Individual Rat Hematologic Values at Three Months. , ...... No. & Hematocrit Hemoglobin Erythrocytes Sex_______ ^______ gms./lQQ ml. (xl06/cmm.) 500 ppm.: Total Leucocytes Neutrophils ' ____ D i f f e r e n t i a l ______________ _____ Lymphocytes Monocytes Eosinophils Basophils (xl03/cmm.) Seg.% Non-Seg.% 7 7o % 7o 14244M 14247M 14252M 14255M 14257M 14260M Mean 48 45 47 44 46 43 45 15.6 14.4 15.1 13.9 15.0 14.0 14.7 7.59 6.91 7.28 6.48 7.27 6.82 7.06 8.18 10.44 8.53 11.35 12.50 15.89 11.15 7 23 9 12 31 13 16 0 0 0 0 0 0 0 92 74 90 86 68 84 82 0 0 0 0 1 2 1 1 3 1 2 0 1 1 0 0 0 0 0 0 0 14224F 14227F 14232F 14236F 14238F 14240F Mean 43 36 41 45 39 38 40 14.2 12.7 14.6 14.6 12.8 12.6 6.14 6.07 6.52 6.39 6.22 5.90 9.40 12.13 12.63 7.02 9.94 9.70 6 18 16 13 6 8 0 0 0 0 0 0 93 82 84 87 94 89 0 0 0 0 0 1 13.6 6.21 10.14 11 0 88 0 1 0 0 0 0 2 1 0 0 0 0 0 0 0 2500 ppm.: 14283M 14287M 14291M 14294M 14297M 14300M Mean 38 35 37 35 41 41 38 12.1 6.20 13.87 6 0 93 1 11.8 11.9 5.29 5.82 8.41 10.18 8 7 0 91 0 92 0 1 11.0 5.15 12.18 11 1 84 0 11.8 6.19 13.55 4 0 94 1 13.4 6.73 11.12 13 1 79 4 12.0 5.90 11.55 8 0 89 1 0 1 0 4 1 3 2 0 0 0 0 0 0 0 14266F 14269F 14272F 14276F 14278F 14281F Mean 31 34 36 34 30 36 33 9.7 10.5 11.6 10.5 9.5 12.0 10.6 5.25 5.04 6.38 5.54 5.42 5.63 5.54 13.37 13.63 8.95 9.45 14.55 8.35 11.38 8 8 10 8 2 10 8 0 91 1 89 0 88 1 89 0 95 0 88 0 90 0 2 2 1 2 1 1 1 0 0 1 1 1 1 0 0 0 0 0 0 0 Company Sanitized. Does notcontain T S C A CB1 oT0ItP)1J CFO- Page 44 Ninety-Day Feeding Study in the Rat. TABLE 18. Individual and Mean Results of Biochemical Studies During Control Period Group Rat No. Alkaline Phosphatase SGOT^ SGPT^ Control, Female Mean 14142 14145 .14149 14153 14156 14154 14144 14147 14152 14155 14158 14160 78 78 75 105 93 45 79 34 24 27 24 29 23 28 23 28 23 30 25 29 24 Control, Male 14162 99 14164 93 14166 57 14172 93 14177 93 14179 99 14170 29 23 14168 30 22 14174 31 24 14176 27 22 14180 32 23 14171 29 26 Mean 89 30 23 100 ppm., Female 14183 36 14188 48 14192 39 14199 48 14182 90 14196 45 14185 26 22 14190 29 23 14193 29 22 14200 29 25 14197 29 24 14195 29 24 Mean 51 29 23 Serum Glutamic Oxalacetic Transaminase Serum Glutamic Pyruvic Transaminase Company Sanitized. Does not contain TSCA CB1 Ninety-Day Feeding Study in the Rat TABLE 18. Continued. Individual and Mean Results of Biochemical Studies During Control Period. Group Rat No. Alkaline Phosphatase SGOT^ SGPT^ 100 ppm., Male Mean 14202 14209 14211 14219 14214 14221 14203 14206 14212 14215 14218 14217 500 ppm., Female 14230 14224 14222 14240 - 14235 14232 14229 14228 14223 14239 14237 Mean 14234 500 ppm., Male Mean 14242 14245 14248 14252 14254 14258 14243 14246 14250 14253 14256 14259 57 87 63 93 96 36 72 90 87 84 96 99 90 91 51 57 54 93 39 87 64 29 23 27 23 30 23 29 23 26 24 30 26 29 23 29 24 25 22 29 22 30 22 28 25 27 22 28 23 27 22 29 22 26 24 29 22 27 22 25 23 27 22 Serum Glutamic Oxalacetic Transaminase Serum Glutamic Pyruvic Transaminase Company Sanitizes. Does no! co.,,, J'SCA Page 46 Ninety-Day Feeding Study in the Rat. TABLE 18. Continued. Individual and Mean Results of Biochemical Studies During Control Period Group Rat No. 2500 ppm., Female 14271 14266 14262 14273 14274 14277 14268 14265 14263 14272 14275 14280 Mean 2500 ppm. , Male Mean 14282 14286 14296 14283 14289 14297 14287 14291 14300 14285 14292 14299 Alkaline Phosphatase 39 75 57 99 78 69 70 90 90 51 51 93 48 71 SGOT1 27 32 28 31 28 29 29 32 27 26 29 29 27 28 SGPT2 22 24 21 24 23 23 23 27 25 24 22 23 21 24 Serum Glutamic Oxalacetic Transaminase O Serum Glutamic Pyruvic Transaminase Company Sanitized. Does not contain TSCA C9! % ] i ! ; ! ^ | : fi) Page 47 NinetyDay Feeding Study in the Rat. TABLE 19. Individual and Mean Results of Biochemical Studies at One Month. Group Rat No. Control, Female Mean 14143 14145 14147 14149 14151 14154 Control, Male Mean 14163 14166 14169 14172 14175 14178 100 ppm., Female Mean 14183 14186 14189 14192 14195 14198 100 ppm., Male Mean 14203 14206 14209 14212 14215 14218 500 ppm., Female Mean 14223 14226 14229 14232 14235 14238 Alkaline Phosphatase 60 84 ' 90 78 96 72 80 72 53 129 63 114 129 . 93 45 75 117 54 108 114 86 84 129 132 105 105 126 114 52 99 84 117 123 102 96 SGOT1 26 28 25 26 24 28 26 25 24 25 28 27 24 26 26 26 25 25 25 25 25 25 27 27 26 26 25 26 28 28 26 27 28 26 27 SGPT2 24 25 23 25 23 25 24 24 21 24 23 24 24 23 23 24 24 22 23 24 23 24 25 24 23 24 23 24 25 25 23 23 26 25 25 Serum Glutamic Oxalacetic Transaminase Serum Glutamic Pyruvic Transaminase Company Sanitized. Does not contain TSCA CBP t i i Page 48 Ninety-Day Feeding Study in the Rat. TAB.LE 19. Continued. Individual and Mean Results of Biochemical Studies at One Month. Group Rat No. 500 ppm., Male . . Mean 14243 14246 14249 14252 14255 14258 2500 ppm., Female 14263 14266 14269 14272 14275 14278 Mean 2500 ppm., Male Mean 14283 14286 14289 14292 14295 14298 Alkaline Phosphatase 72 132 132 . 96 87 132 109 84 51 60 72 84 45 66 . 72 117 117 69 105 81 94 SGOT1 27 27 26 26 25 25 26 28 27 26 28 28 28 28 28 27 24 25 26 25 26 SGPT 23 25 24 24 23 25 24 24 24 23 24 24 25 24 23 24 23 23 23 24 24 Serum Glutamic Oxalacetic Transaminase Serum Glutamic Pyruvic Transaminase Company Sanitized. Doss not contain TSCA CBi Ninety-Day Feeding Study in the Rat. TABLE 20. Individual and Mean Results of Biochemical Studies at Two Months. Group Rat No. Control, Female Mean 14144 14148 .14150 14151 14153 14155 Control, Male Mean 14163 14166 14169 14171 14172 14173 100 ppm., Female Mean 14183 14186 14187 14192 14195 14198 100 ppm., Male Mean 14203 14206 14209 14211 14216 14219 1000 ppm., Female 14224 14227 14229 14232 14235 14236 Mean Alkaline Phosphatase 60 73 59 56 67 67 64 54 38 79 98 78 78 71 31 47 58 39 36 37 41 56 36 111 64 33 46 58 62 58 64 63 46 27 53 SGOT1 SGPT' 27 21 26 24 25 - 22 19 22 25 22 26 22 25 22 28 20 28 23 26 23 27 24 24 24 25 22 26 23 24 21 23 23 25 22 24 21 30 22 29 22 26 22 25 24 28 21 27 22 27 22 29 24 29 23 28 23 26 21 27 22 29 24 26 22 31 22 25 21 27 22 Serum Glutamic Oxalacetic Transaminase Serum Glutamic Pyruvic Transaminase Company Sanitized. Doesno.conmin t s c a c b , Page 50 Ninety-Day Feeding Study in the Rat. TABLE 20. Continued. Individual and Mean Results of Biochemical Studies at Two Months. Group Rat No. 1000 ppm., Male . Mean 14242 14247 14249 14254 14256 14259 5000 ppm., Female 14264 14267 14269 14271 14274 14277 Mean 5000 ppm., Male Mean 14284 14289 14292 14294 14296 14298 ' Alkaline Phosphatase 39 45 . 53 41 38 70 48 64 32 32 36 28 37 40 47 49 82 27 50 88 57 SGOT1 26 27 29 26 29 28 28 27 27 26 27 29 28 27 26 27 27 27 29 28 27 SGPT2 23 22 21 23 21 22 22 20 21 21 21 21 22 21 23 21 21 20 24 23 22 Serum Glutamic Oxalacetic Transaminase Serum Glutamic Pyruvic Transaminase Company Sanitized. Does not contain TSCACBi ! j i 1 <t> i Page 51 TABLE 21. Ninety-Day Feeding Study in the Rat. Individual and Mean Results of Biochemical Studies at Three Months. Group Rat No. Alkaline Phosphatase SGOT1 SGPT2 Control, Female Mean 14144 14248 14155 14157 14159 14160 Control, Male Mean 14174 14171 14173 14175 14177 14180 TOO ppm., Female . Mean 14187 14189 14192 14196 14198 14199 100 ppm., Male Mean 14204 14207 14209 14211 14213 14216 1000 ppm., Female 14226 14229 14233 14235 14237 Mean 14239 26 59 21 20 31 28 31 24 30 32 79 77 107 58 22 29 18 14 68 32 31 16 25 47 25 35 41 32 39 53 73 55 50 22 49 30 . . 23 26 28 27 28 27 26 26 27 25 27 28 27 ' 25 26 25 22 23 24 24 24 23 26 25 24 22 24 23 26 25 25 25 23 25 23 24 22 22 23 26 23 21 23 22 25 24 27 24 22 23 21 21 22 21 22 22 21 20 21 21 9? 21 24 24 23 25 22 20 23 Serum Glutamic Oxalacetic Transaminase 2 Serum Glutamic Pyruvic Transaminase Company Sanitized. Does not contain TSCA CK Page 52 Ninety-Day Feeding Study in the Rat. TABLE 21. Continued. Individual and Mean Results of Biochemical Studies at Three Months. Group Rat No Alkaline Phosphatase SG0T* SGPT2 1000 ppm., Male Mean 14243 14250 14254 14256 14259 14261 5000 ppm., Female 14264 14277 14271 14274 14280 Mean 14279 5000 ppm., Male Mean 14284 14286 14290 14292 14296 14298 41 43 15 26 87 34 41 26 19 18 18 26 16 21 49 66 37 75 45 33 51 29 25 26 23 23 22 25 25 25 25 26 28 25 26 27 25 24 24 24 24 . 25 27 22 19 20 23 22 22 22 21 20 22 22 19 21 24 24 21 22 22 21 22 Serum Clutamic Oxalacetic Transaminase Serum Glutamic Pyruvic Transaminase Company Sanitized. Does not contain TSCA CBl Sex Volume (ml. ) Appear ance pH Specific Gravity Albumin Bili- Glu- Occ. Epi. rubin cose Blood' WBC RBC Cells Amor. Urates Amm. Triple Urates Phos. Calcium Bact- Ox. eria Blae dei Worn L43 F 5 S;C 7.0 1.028 N 146 F 12 LS; cl 6.9 1.030 N 150 F 3 S;cl 6.3 1.032 N .54 F 5 S;C 6.8 1.032 N .57 F .60 F 6 S;C 7.0 1.032 N 6 S;C 7.2 1.030 N N N N N N N N N OCC NN NN NN N NN .63 M 21 LS;C 6.5 1.030 N .67 M .73 M 1 Am; C 6.4 1.063 3+ 1 LAm;C 6 . 2 1.065 N .75 M .78 M 4 S;cl 6.8 1.040 N 2 S ;cl 6.4 1.045 N .81 M 12 LS;C 9.0 1.030 N N N N N N N NN N N occ NN N N occ NN NN 1 ppm. : occ 1-3 F F F M F FM F F F F F F M M F M F F F F M M F 84 F 89 F 91 F 94 F 98 F 01 F 04 M 08 M 10 M 05 M 16 M 20 M * 6 LS; cl 6.8 7 LS ;cl 8.8 4 S;C 6.5 3 S;cl 8.8 13 LS;C 9.0 3 LAm;C 6.8 6 LS;cl 8.5 3 S ;C 6. 2 2 S;C 6.5 4 S;cl 6.8 4 LS ;C 7.0 9 LS;cl 9.0 Straw Light Straw Amber Light Amber 1.030 1.030 1.030 1.040 1.030 1.045 1.030 1.055 1.042 1.030 1.032 1.030 Clear Cloudy N N N N o.cc N N NN N N N N occ N N NN N N NN N N NN N - Negative 1H-- Trace-to-Slight 2+ - Slight-to-Moderate 3+ - Moderate 4+ - Marked F F F occ M F occ F FF occ M occ F F M F F M M F F M F F F M F F F F F Few Many Occasional emoany Sanfflzed. Does not contain TSC CB! Page 53 Ninety-Day Feeding Study in the Rat. LE 22. continued. Urinalysis Values for Male and Female Rats during Control Period'; 41 F 44* M 47 M 49 M 51 M 55 M 57 M ppm. 54 F 57 F 59 F 76 F 78 F 51 F 54 M ?8 M 0 M >5 M 18 M 1 M S LS DS * Am LAm 1 S;C 6.3 3 Sjcl 8.8 3 S;C 6.3 3 S;cl 8.0 7 LS;cl 6.5 3 LAm;C 6 .0 4 S;C 6 . 2 4 S ;cl 9.0 5 S;cl 8.3 4 S;cl 6.7 6 LS;cl 6.7 4 S;cl 6.5 1 AmjC 6 S;cl 21 LS;C 4 S; cl 4 S;C 2 DS;C 2 S;C 2 S;cl 5 S;cl 10 LS;cl 12 S; cl 1 Am;C Straw Light Straw Dark Straw Amber Light Amber 6.5 6.9 7.0 7.3 7.4 9.0 7.0 9.0 9.0 9.0 6.9 6.2 G cl 1.045 1.042 1.045 1.040 1.030 1.045 1.040 1.035 1.032 1.045 1.030 1.042 1.065 1.030 1.030 1.030 1.033 1.042 1.048 1.053 1.037 1.030 1.030 1.055 Clear Cloudy N N NN N N NN N N NN N N NN N N NN N N NN N N NN N N NN N N NN N N NN N N NN N N NN N N NN N N NN N N NN N N NN N N NN N N NN N N NN N N NN N N NN N N NN N N NN N N N.N N - Negative 1+ - Trace-to- Slight 2+ - Siight-to-Moderate 3+ - Moderate 4+ - Marked CM r1H occ F M F F F FM F FF FF FMF occ M M F F OCC occ occ occ M F F M F M F F F M M M M M occ occ occ F F F MM F occ O C C occ M occ MM FF F M F M F F F occ M occ M occ occ occ 2-3 M .F M F M M F M M F M L F F - Few M - Many L - Loaded * Occasional uric acid occ - Occasion ,1 contain T S C ftC B I Comp!m, S 3 * e d . D o n o 03 0(QD Ln -O \ LE 23. Ninety-Day Feeding Study in the Rat, Urinalysis Values for Male and Female Rats at One Month. t Volume Appear- Specific . Sex (ml.) ance pH Gravity trol : 62 M 12 LS;cl 8.5 1.013 65 M 12 LS;cl 7.9 1.016 6 8 M 17 LS-;cl 8.9 1.014 71 M 4 LS;cl 7.2 1.048 74 M 15 LS ;cl 7.0 1.013 77 M 9 LS ;cl 6.9 1.020 42 F 10 LS ;cl 6.5 1.017 45 F 7 S;cl 9.0 1.041 46 F 15 LS; cl 7.1 1.012 .48 F 6 DAm;C 8.9 1.031 50 F 12 LS;cl 6.2 1.011 53 F 7 S;cl 6.9 1.035 t ppm. : :02 M 7 S;cl 7.0 1.044 :05 M 26 LS;cl 7.3 1.008 :08 M 10 S;cl 6.9 1.021 '.11 M 16 LS;cl 9.0 1.009 :i4 M 14 S;cl 6.5 1.013 -17 M 10 S;cl 6.3 1.021 .82 F 4 S;cl 7.0 1.042 .85 F 13 LS; cl 7.1 1.011 .88 F 14 LS;C 9.0 1.007 .91 F 12 LS;cl 6.8 1.016 .94 F 7 S;cl 9.0 1.019 .97 F 9 S ;cl 6.5 1.017 Le: S - Straw LS - Light Straw DAm - Dark .Amber C - Clear cl - Cloudy m It Microscopic_________ ____________ Albu- Bili-- Glu- Occ. Epi. Amor. Amm. Oai- Blad- Triple cium Bact- der min rubin cose Blood WBC RBG Gells Urates Urates Phos. Ox. eria Worm: NN N .N NN NN NN NN NN NN NN NN NN NN NN NN NN NN NN NN NN NN NN NN NN NN CM r--11 CM r--1 occ 1-2 F OCC 1-2 F F F F F F occ F FF M occ M M FF FM M FF F M MF FF FF M FM N N NN N N NN N N NN N N NN N N NN N N NN N N NN N N NN N N NN N N NN N N NN N N N. N N - Negative 1+ - Trace-to- Slight 2+ - Slight-to-Moderate 3+ - Moderate 4+ " Marked FFF FM F occ M 1-2 F occ F F FL occ F FF F occ F M occ F F M F F M M M F F F M F M F - Few M - Many L - Loaded occ - Occasional Gompany Sanitized. Does n ot contain T S C A C03 OCC occ occ Q> U0L>ni Ninety-Day Feeding Study in the Rat TABLE 23. Continued Urinalysis Values for Male and Female Rats at One Month. Rat Volume Appear- Specific Albu- No. Sex (ml.) ance pH Gravity min Billrubin ________________________ Microscopic __________________ Cal- Blad- Glu- Occ. Epi. Amor. Amm. Triple cium Bact- der cose Blood WBC RBG Cells Urates Urates Phos. Ox. eria Worm; 500 ppm.: 14242 14245 14248 14251 14254 14257 M M M M M M 14222 14225 14228 14231 14234 14237 F F F F F F 12 10 12 13 16 17 8 4 6 14 3 16 LS; cl LS; cl LS; cl LS;cl LS; cl LS; cl 7.1 9.0 9.0 6.1 6.8 8.9 S;cl S; cl S;cl S;cl DS; cl LS; cl 6.5 6.5 6.6 7.2 6.1 8.8 1.012 1.023 1.011 1.015 1.011 1.012 1.024 1.048 1.034 1.012 1.050 1.010 N N N N N N N N N N N N N NN N NN N NN N NN N NN N NN N NN N N N N NN N NN N N N OCC N NN CM T--1< F 1-2 F M F 1-2 F F FF FM FF occ F F occ F M F FL F F M occ M M M occ M F F F F F 2500 ppm.: 14282 M 14 LS;C 7.1 1.023 N N N N 14285 M 7 LS; cl 7.3 1.035 N N N N 14288 M 17 LS; cl 8.8 1.015 N N N N 14291 M 14 LS; cl 9.0 1.012 N N N N 14294 M 4 DS; cl 8.9 1.042 N N N N 14297 M 17 S;cl 6.9 1.014 N N N N 14262 F 4 S;cl 7.0 1.044 N N N N 14265 F 4 S;cl 6.1 1.040 N N N N 2-3 14268 F 6 S;cl 6.5 1.025 N N N N 14271 F 15 LS;cl 7.0 1.011 N N N N 14274 F 14 S;cl 6.7 1.013 N N N N 14277 F 8 S;cl 7.1 1.017 N N N N FM 1-2 F F L FF occ M FF occ F F FF FF OCC F occ F M Code: S - Straw LS - Light Straw DS - Dark !Straw C - Clear cl - Cloudy N - Negative 1 + - Trace-to- Slight 2+ - Slight-to-Moderate 3+ - Moderate 4+ - Marked F - Few M - Many L - Loaded occ - Occasion! F F OCC F F OCC F F OCC F M F M M M T<D) ((TDO Ln ON Company Sanitized. Does not contain T S C A CBI Ninety-Day Feeding Study in the Rat. TABLE 24. Urinalysis Values for Male and Female Rats at- Two Months. Microscopic Cal- Blad" Rat Volume Appear- Specific Albu- Bili- Glu- Occ. Epi. Amor. Amm. Triple eium Bact- der No. Sex (ml.) ance pH Gravity min rubin cose Blood WBC BBC Cells Urates Urates Phos. Ox. eria Wcrrmf Control: 14164 M 28 LS;cl 7.0 1.015 N N N N 14167 M 16 LS;cl 7.1 1.022 N N N N 14170 M 12 LS; cl 7.0 1.028 N N N N 14174 M 25 LS;cl 9.0 1.014 N N N N 14178 M 24 LS;cl 7.5 1.012 N N N N 14181 M 30 LS;cl 8.0 1.009 N N N N OCC F 14143 F 6 LS;cl 7.5 1.030 N N N N 14147 F 13 LS;C 7.7 1.022 N N N N 14149 F 6 S;cl 9.0 1.030 N N N N 14152 F 11 LS;cl 9.0 1.024 N N N N 14154 F 3 S;C 6.2 1.065 N N N N F 14158 F 4 S;C 6.0 1.047 N N N N :f occ MM MF MF F MF F FF FM FM FL occ M FF M IF M M IF F M M 1? M M F F F F 100 D D m . : 14204 14210 14213 14217 14220 14221 M M M M M M 14184 14190 14193 14194 14196 14200 F F F F F F 30 18 21 17 30 23 4 20 24 6 5 8 LS ;C , LS;cl LS;cl S;cl LS;cl LS;cl 7.8 8.8 9.0 9.0 8.0 9.0 S;cl LS;cl LS;cl S;cl S;cl S;cl 7.5 8.4 6.4 9.0 6.8 6.2 1.016 1.022 1.016 1.015 1.008 1.011 1.044 1.008 1.006 1.028 1.028 1.030 Code: S - Straw - C - Clear LS - Light Straw cl - Cloudy N N NN N N N .N N N NN N N NN N N NN N N NN N N NN N N NN N N NN N N NN N N NN N N NN N - Negative 1+ - Trace-to-Slight 2H-- Slight-to-Moderate 3+ - Moderate 4+ - Marked OCC occ occ F F F M F - Few M - Many L - Loaded occ - Occasional MM FF MF F FF FF MM FM M OCC FM FF F FF MT FF FF M IF F F F M M M F 0) O(QD t_n Company Sanitized. Does not contain T S C A CBt XNinety-Day Feeding Study in the Rat. TABLE 24. Continued. Urinaysis Values for Male and Female Rats at Two Months Microscopic______________________ Cal- Blad- Rat Volume Appear- Specific Albu- Bili- Glu- Occ. Epi- Amor. Amm. Triple cium Bact- der No. Sex (ml.) ance pH Gravity min rubin cose Blood WBC RBC Cells Urates Urates Phos. Ox. eria Worm: 1000- ppm. : 14243 M 24 LS;cl 9.0 1.013 N N N N F 14246 M 16 LS;cl 6.8 1.017 N N N N 14251 M 12 LS;cl 6.0 1.026 N N N N occ F 14253 M 30 LS;C 7.8 1.010 N N N 1+ mu M M l LS:cl LSjcl hi 1.013 1.015 N N N N NN NN 14223 F 11 S;cl 7.2 1.013 N N N N F 14226 F 10 S;cl 9.0 1.030 N N N N 14230 F 12 LS;cl 8.8 1.016 N N N N F 14234 F 7 S;cl 6.4 1.023 N N N N F 14237 F 14 LS;C 7.5 1.010 N N N N 14240 F 15 LS;cl 7.8 1.009 N N N N FF FM FF FF FF FF M OCC FM FF FF F f: FM F F F F M M F M F M M E M 5000 ppm. : 14283 M 14 LS;cl 9.0 1.021 N N N N 14289 M 27 LS;cl 9.0 1.013 N N N N 14293 M 10 S;cl 9.0 1.034 N N N N 14295 M 40 LS;cl 9.0 1.006 N N N N 14297 M 27 LS;cl 9.0 1.012 N N N N 14300 M 23 LS;cl 6.7 1.010 N N N N 14263 F 12 S;cl 7.0 1.020 N N N N 14270 F 10 S;cl 7.2 1.017 N N N N 14273 F 14 LS;cl 9.0 1.013 N N N N 14275 F 5 Am;cl 8.5 1.024 N N N N 14279 F 8 S;C 6.0 1.026 N N N N 14281 F 4 S;C 8.2 1.028 N N N N occ F Code: S - Straw C - Clear LS - Light Straw c \ - Cloudy Am - Amber N - Negative 1+ - Trace-to- Slight 2+ - Slight-to-Moderate 3+ - Moderate 4+ - Marked F - Few M - Many L - Loaded occ - Occasional occ F M F F F F F F F F M F F F F F M F F M M F F FF FF FF FM FF FF F F F M F F 0> (D Ln OO Company Sanitized. Does not contain T S C A C B l Ninety-Day Feeding Study in the Rat. Urinalysis Values for Male and Female Rats at Three Months Microscopic Cal- Blad- Rat Vo lume Appear- Specific Albu- Bili- Glu- Occ. Epi. Amor. Amm. Triple cium Bact- der No. Sex (ml.) ance pH Gravity min rubin cose Blood WBC RBC Cells Urates Urates Fhos. Ox. eria Worm, Control: 14164 M 8 LS;cl 6.8 1.053 N N N N 14169 M 7 S;cl 7.3 1.055 N N N N 14172 M 11 LS;cl 9.0 1.035 N N N N 14176 M 5 S;cl 6.5 1.066 N N N N 14179 M 4 S;cl 7.8 1.058 N N N N 14181 M 7 S;cl 6.9 1.052 N N N N 14147 F 1 S;C 6.1 1.080 N N N N 14151 F 2 S;C 6.3 1.069 N N N N 14154 F 2 S;cl 7.4 1.065 N N N N 14156 F 2 S;cl 6.1 1.065 N N N N 14158 F 1 S;C 6.0 1.080 N N N N 14161 F 2 S;C 6.7 1.065 N N N N occ FM FFM FF 1-2 F M FFM FM FF FM occ F M FFF 1-2 F F occ F M M J? F ;F M :f FT FT F 3? F F M F F F 100 ppm.: 14203 M 7 S;cl 6.7 1.058 N N N N 14206 M 7 LS;cl 7.0 1.048 N N N . N 14210 M 6 S;cl 9.0 1.055 N N N N 14215 M 3 DS;cl 6.0 1.080 N N N N 14218 M 9 S;cl 9.0 1.045 N N N N 14219 M 6 LS;cl 6.3 1.049 N N N N 14183 F 1 S;C 6.0 1.080 N N N N 14186 F 6 LS;cl 8.0 1.045 N N N N 14190 F 6 S;cl 7.0 1.035 N N N N 14195 F 6 LS;cl 6.2 1.035 N N N N 14197 F 1 S;C 7.7 1.072 N N N N 14200 F 2 S;cl 6.0 1.065 N N N N O C C FF FFF 2-3 F FF FF occ M FF FL 1-2 F F FF MF FM M M FF MF F :f F F F M M F F Code: S - Straw LS - Light Straw DS - Dark :Straw 9 C - Clear cl - Cloudy N - Negative 1+ " Trace-to-Slight 2+ - Slight-to-Moderate 3+ - Moderate 4+ - Marked F - Few M - Many L - Loaded occ - Occasional Company Sanitized. Does not contain T S C A CBI JD O<QD Ln K Ninety-Day Feeding Study in the Rat. TABLE 25. Continued. Urinalysis! Values" for Mle, and: Female :Rats at Three Months. Microscopic Cal- Blad- Rat Volume Appear- Specific Albu- Bili- Glu- Occ. Epi. Amor. Amm. Triple cium Bact- der No. Sex (ml.) ance pH Gravity min rubin cose Blood WBC RBC Cells Urates Urates Phos. Ox. eria Woxmi 1000 ppm; : 14244 M 10 LS; cl 7.0 i.035 N N N 3+* 14247 M 6 S;cl 6.8 1.057 N N N N 14252 M 5 S;cl 6.6 1.065 N N N N 14255 M 4 S;C 6.0 1.065 N N N N 14257 M 10 LS;cl 9.0 1.037 .N N N 14260 M 3 S;cl 6.7 1.073 N N N N OCC F F F 2-3 F F OCC F F F 2-3 F M 1-2 F M 14224 F 5 S;cl 9.0 1.045 N N N N 14227 F 2 S;C 6.7 1.068 N N N N 14232 F 3 S;cl 5.9 1.057 N N N N OCC 14236 F 2 S;C 6.0 1.075 N N N N 1-2 14238 F 2 S;cl 6.5 1.065 N N N N 14240 F 1 S;C 6.2 1.078 N N N N FF FF FF FF FF FF MW M :f F ,F F MH F M F M F F F 5000 ppm.: 14283 14287 14291 14294 14297 14300 M M M M M M 14266 14269 14272 14276 14278 14281 F F F F F F 2 4 6 2 4 4 1 2 1 1 2 1 Am; Cl S;cl S;cl DS;cl DS;C S ;cl S;C S;cl DS;C DS;C S;C DS;C 6.2 6.8 8.8 7.7 6.1 6.8 7.0 6.2 5.8 6.6 6.3 6.0 1.075 1.073 1.045 1.080 1.075 1.065 1.080 i.068 1.065 1.065 1.065 1.080 Code: S - Straw LS - Light Straw DS - Dark ,Straw Am - Amber C - Clear cl - Cloudy ) N N NN N N NN 2+ N N 'N N N NN N N NN N N NN N N NN N N NN N N NN N N NN N N NN N N NN N - Negative 1+ - Trace-to-Slight 2+ - Slight-to-Moderate 3+ - Moderate 4+ - Marked occ F F F FF FM FM OCC F F FFF FF occ F M OCC F F M 1-2 F M 1-2 F M 1-2 F F F - Few M - Many L - Loaded OCC - Occasional * Repeat 3+ F F m F F F F F F F F M U OQ F :f F :f M O Company Sanitized. Does not contain T S C A CBI Ninety-Day Feeding Study in the Rat. Rats were normal except as noted below: Control: 14165 14146 M F 100 PESI: 14202 14208 14185 M M F 2500 DDm. : 14288 14262 M F thymus uterus lung lung uterus spleen spleen Few petechial hemorrhages. Mild hydrouterus. Moderate pneumonia. Mild pneumonia. Mild hydrouterus. Slightly enlarged. Slightly enlarged. <* m ) Company Sanitized. Does not contain TSCA CBI Page 62 % Ninety-Day Feeding Study in the Rat. TABLE-26- Continued. Necropsy Observations. Sixty-Day Interim Sacrifice. Animal Number Sex Organ Comment Rats were normal except as noted below: Control: 14149 F lung Few scattered gray areas. 100 D D m . : 14221 M liver lung Pale. Gray pinpoint areas scattered throughout. 5000 ppm.: 14289 M 14293 14295 M M liver testes liver liver Slightly pale. Left testis approximately twice the size of right. Pale. Pale yellow in color. Company Sanitized. Does not contain T S C A CBI Page 63 Ninety-Day Feeding Study in the Rat. TABLE 26. Continued. Necropsy Observations. Ninety-Day Terminal Sacrifice. Animal Number Sex Organ Comment Rat were normal except as noted below: Controlr 14175 14153 M F 100 DDm.: 14214 M 1000 DDm. : 14252 14254 14227 M M F- 14233 14237 F F 5000 D D m . : 14284 M 14286 .M 14291 M 14294 M 14297 M 14266 F 14274 F lung lung lung liver lung abdominal cavity lung lung liver lung liver liver liver lung kidney Mild pneumonia. Mild pneumonia. Mild pneumonia. Slightly yellowish. Mild pneumonia. 6 mm. firm hemorrhagic area in abdominal fat Mild pneumonia. - Mild pneumonia. Slight yellowish cast. Mild pneumonia. Slight yellowish cast. Slight yellowish cast. .Slight yellowish cast. Moderate pneumonia and bronchiectasis. Hydronephorsis, right kidney. Company Sanitized. Does not contain TS CA CBI Page 64 Animal Number Sex Organ Comment Pats were normal except as noted below: 5000 ppm.: 14299 14300 M M liver liv e r Slightly pale. Slightly pale. Company Sanitized. Does not contain TSCA CBI age 65 Ninety-Day Feeding Study in the Rat. 27. Mean Actual (Grams) and Relative (X Body Weight) Organ Weights. Terminal Dietary Body Wt. Level Sex ,,Gm,,.__ Gm. 30-Day Interim Sacrifice: 0 0 100 100 500 500 2500 2500 M 315 0.97 F 202 0.70 M 268 0.70 F 212 0.69 M 243 0.62 F 188 0.47 M 277 1.12 F 173 0.59 60-Dav Interim Sacrifice: 0 M 355 0.76 0 F 233 0.52 100 M 338 0.79 100 F 230 0.52 j|000 M 355 0.74 ^000 F 210 0.54 5000 M 412 0.90 5000 F 232 0.62 90-Day Terminal Sacrifice: 0 M 495 1.08 0 F 270 0.71 100 M 469 1.14 100 F 284 0.81 1000 M 452 0.97 1000 F 284 0.77 5000 M 425 0.98 5000 F 237 0.81 21-Day Compound Withdrawal: 0 M 490 0.70 0 F 297 0.56 1000 M 507 0.91 1000 F 287 0.61 5000 M 440 0.61 5000 F 277 0.57 % Gm. % Gm. % Gm. % Gm. 0.308 0.347 0.261 0.326 0.255 0.250 0.404 0.341 14.11 8.74 11.65 7.28 10.06 8.26 15.68 8.65 4.479 4.327 4.347 3.434 4.136 4.394 5.661 5.000 0.039 0.069 0.044 0.054 0.037 0.054 0.044 0.068 0.010 0.034 0.020 0.025 0.015 0.029 0.016 0.039 2.66 1.73 2.22 1.56 2.30 1.86 2.80 1.85 0.844 0.856 0.828 0.736 0.946 0.990 1.011 1.069 2.99 0.949 0.089 0.040 2.69 1.003 0.092 0.040 2.74 1.127 0.097 0.050 2.74 0.989 0.123 0.070 0.214 0.22 0.234 0.226 0.208 0.257 0.218 0.267 13.02 8.25 10.91 8.00 12.94 7.89 21.71 12.04 3.668 0.062 3.541 0.070 3.228 0.048 3.478 0.065 3.645 0.046 3.757 0.075 5.269 0.071 5.190 .0.067 0.017 0.030 0.014 0.028 0.013 0.036 0.017 0.029 2.62 1.72 2.53 1.70 2.70 1.92 3.46 2.36 0.738 0.738 0.749 0.739 0.761 0.914 0.840 1.017 3.07 0.865 0.133 0.060 3.30 0.976 0.132 0.060 3.08 0.868 0.162 0.080 3.82 0.927 0.145 0.060 0.218 0.263 0.243 0.285 0.215 0.271 0.231 0.342 14.12 8.17 14.19 8.27 15.02 10.59 20.38 10.95 2.853 3.026 3.026 2.912 3.323 3.729 4.795 4.620 0.066 0.075 0.057 0.071 0.060 0.087 0.057 0.072 0.013 0.028 0.012 0.025 0.013 0.031 0.013 0.030 2.87 1.73 2.95 1.87 3.05 2.30 3.33 2.25 0.580 0.641 0.629 0.658 0.675 0.810 0.783 0.949 3.32 0.670 0.154 0.057 3.39 0.723 0.171 0.060 3.39 0.750 0.155 0.055 3.37 0.793 0.142 0.060 0.143 0.189 0.179 0.213 0.139 0.206 14.82 9.17 17.41 9.35 16.55 10.75 3.024 3.087 3.434 3.258 3.761 3.881 0.056 0.073 0.065 0.077 0.058 0.083 0.014 0.025 0.013 0.027 0.013 0.030 2.97 2.09 3.36 2.25 3.18 2.45 0.606 0.704 0.663 0.784 0.723 0.884 3.36 0.686 0.224 0.080 3.59 0.709 0.177 0.060 3.32 0.755 0.162 0.060 D Company Sanitized. Does not contain T S C A CBI Thymus Gm. 1 Heart Gm. . 1 Lung Gm. Thyroid Brain % Gm. % Gm.. 1 Pituitary Gm. % 0 .90 0 .68 0 .73 0 .47 0 .67 0 .71 0 .74 0 .59 0 .286 0 .337 Q,.272 0,.222 0..276 0,.378 0,.267 0,.341 1 .15 0 .78 0 .98 0 .69 0 .94 0 .77 1.06 0 .68 0. 365 0. 386 0.,366 0.,325 0. 386 0.410 0. 383 0. 393 1.63 1.32 1.56 1.25 1.58 1.28 1.65 1.34 0.517 0.653 0.582 0.590 0.650 0.681 0.596 0.774 0.023 0.021 0.027 0.018 0.024 0.024 0.018 0.018 0.007 0.010 0.010 0.008 0.010 0.013 0.006 0.010 1.86 1.79 1.74 1.74 1.84 1.74 1.96 1.80 0.591 0.886 0.649 0.820 0.757 0.926 0.708 1.040 0.009 0.007 0.009 0.009 0.008 0.008 0.006 0.004 0.003 0.003 0.003 0.004 0.003 0.004 0.002 0.002 0,.64 0 .57 0 .79 0,.50 0..66 0,.56 0,.90 0..56 0..180 0,.244 0..234 0,.217 0..186 0,.267 0..218 0..241 1.41 0 .86 1 .17 0 .76 1 .09 0 .81 1 .36 0 .85 0.,397 0.,369 0.,346 0.,330 0.,307 0.,386 0.,330 0.,366 1.91 1.16 1.70 1.44 1.92 1.41 2.06 1.34 0.538 0.498 0.503 0.626 0.501 0.671 0.500 0.578 0.026 0.024 0.027 0.036 0.022 0.033 0.030 0.025 0.007 0.010 0.008 0.016 0.007 0.016 0.007 0.011 1.61 1.79 1.88 1.75 2.00 1.87 1.98 1.86 0.454 0.768 0.556 0.761 0.563 0.890 0.481 0.802 0.014 0.011 0.010 0.012 0.010 0.010 0.012 0.013 0.004 0.005 0.003 0.005 0.003 0.005 0.003 0.006 0..66 0,.51 0.,64 0. 52 0. 59 0. 50 0. 66 0. 50 0. 130 0.,189 0.,136 0. 183 0. 131 0. 176 0. 155 0. 211 1 .37 0 .84 1 .42 0,.87 1,.32 0..98 1,.29 0..85 0. 277 0. 311 0. 303 0. 306 0. 292 0. 345 0. 304 0. 359 2.02 1.37 2.11 1.40 2.08 1.48 1.91 1.54 0.408 0.507 0.450 0.493 0.460 0.521 0.449 0.650 0.028 0.025 0.027 0.024 0.028 0.031 0.025 0.022 0.006 0.009 0.006 0.008 0.006 0.011 0.006 0.009 1.92 1.82 1.99 1.87 1.96 1.89 2.00 1.85 0.388 0.674 0.424 0.658 0.434 0.665 0.471 0.781 0.014 0.013 0.011 0.013 0.013 0.013 0.010 0.010 0.003 0.005 0.002 0.005 0.003 0.005 0.002 0.004 0. 79 0. 52 0. 84 0. 58 0. 67 0. 62 0. 161 0. 175 0. 166 0. 202 0. 152 0. 224 1..32 0..89 1.,51 0.,84 1.,28 0.,93 0. 269 0. 300 0. 298 0. 293 0. 291 0. 336 2.47 1.53 2.29 1.63 1.82 1.60 0.504 0.515 0.452 0.568 0.414 0.578 0.035 0.026 0.036 0.029 0.030 0.030 0.007 0.009 0.007 0.010 0.007 0.010 2.07 1.87 2.12 1.95 2.01 1.90 0.422 0.630 0.418 0.680 0.457 0.686 0.010 0.012 0.014 0.014 0.012 0.012 0.002 0.004 0.003 0.005 0.003 0.005 Company Sanitized. Does not contain T S C A CB! Ninety-Day Feeding Study in the Rat TABLE 28. Organ Weights, Grams Animal Terminal No. & Weight Soleen GrouD Sex Grams 30-DAY INTERIM SACRIFICE: Control: 14162 M 14165 M 14168 M 14142 F 14145 F 14146 F 270 360 315 205 175 225 1.09 0.92 0.89 0.52 0.99 0.60 100 D D m . 14202 14205 14208 14182 14185 14188 M M M F F' F 275 300 230 280 190 165 0.94 0.67 0.50 0.57 1.10 0.40 500 onm, 14242 M 14245 M 14248 M 14222 F 14225 F 14228 F 225 270 235 205 160 200 0.49 0.79 0.59 0.50 0.40 0.50 2500 DDm.: 14282 M 14285 M 14288 M 14262 F 14265 F 14268 F 255 290 285 195 170 155 0.71 0.90 1.76 0.99 0.47 0.32 , Liver Adrenals 12.02 17.02 13.30 8.63 8.11 9.49 0.024 0.051 0.043 0.079 0.056 0.071 12.51 13.42 9.02 7.75 7.81 6.28 0.044 0.050 0.038 0.060 0.053 0.048 9.49 11.02 9.66 9.20 7.08 8.51 0.028 0.046 0.036 0.052 0.051 0.060 14.75 15.29 16.99 10.35 7.78 7.82 0.061 0.051 0.019 0.079 0.056 0.068 Kidnevs 2.35 3.11 2.52 1.79 1.50 1.91 2.39 2.41 1.86 1.61 1.67 1.42 2.16 2.52 2.21 1.96 1.59 2.04 2.49 2.90 3.00 2.12 1.70 1.74 Testes/ Ovaries 3.06 2.81 3.10 0.084 0.063 0.120 2.76 3.11 2.19 0.110 0.092 0.075 2.83 2.82 2.56 0.084 0.083 0.123 2.29 2.83 3.10 0.100 0.117 0.153 Thymus 0.75 0.93 1.01 0.79 0.53 0.71 0.81 0.69 0.70 0.60 0.41 0.39 0.50 0.93 0.58 0.80 0.45 0.87 0.65 0.88 0.70 0.67 0.61 0.50 Heart 0.90 1.43 1.12 0.79 0.71 0.85 0.93 1.07 0.93 0.72 0.65 0.70 0.99 1.03 0.79 0.81 0.70 0.80 0.96 1.02 1.20 0.76 0.65 0.63 Lune Thyroid 1.44 1.68 1.76 1.30 1.22 1.45 0.020 0.024 0.025 0.022 0.020 0.021 1.84 1.47 1.37 1.30 1.36 1.08 0.025 0.027 0.029 0.030 0.012 0.012 1.76 1.60 1.38 1.31 1.14 1.38 0.033 0.023 0.016 0.028 0.026 0.018 1.46 1.40 2.08 1.41 1.14 1.47 0.026 0.019 0.010 0.021 0.013 0.020 Brain 1.84 1.83 1.92 8.80 1.78 1.80 1.90 1.72 1.61 1.70 1.72 1.79 1.73 2.00 1.78 1.71 1.71 1.79 1.93 2.01 1.94 1.81 1.75 1.83 Pituitary 0.007 0.010 0.008 0.008 0.006 0.007 0.009 0.008 0.010 0.010 0.010 0.007 0.006 0.010 0.007 0.007 0.007 0.009 0.005 0.006 0.007 0.003 0.002 0.006 Page 67 Company Sanitized. Does not contain TSCA CBI Ninety-Day Feeding Study in the Rat. "t a b l e 2.8. Continued. Organ Weights , Grams. Animal Terminal No. & Weight Soleen GrouD Sex Grams 21-DAY COMPOUND WITHDRAWAL: Control: 14177 M 14179 M 14180 M 14158 F 14159 F 14161 F 420 520 530 280 320 290 / 0.60 0.65 0.85 0.42 0.53 0.73 1000 DDm. 14258 M 14259 M 14260 M 14238 F 14239 F 14240 F 5000 DDm, 14298 M 14299 M 14300 M 14278 F 14279 F 14280 F 560 470 490 280 290 290 340 535 445 280 260 290 1.05 0.63 1.05 0.68 0.57 0.59 0.55 0.78 0.50 0.57 0.53 0.62 Liver Adrenals 11.80 14.98 17.68 8.48 9.34 9.70 0.050 0.064 0.055 0.071 0.072 0.075 18.65 17'.40 16.17 8.90 9.63 9.52 0.064 0.068 0.062 0.081 0.069 0.081 11.10 22.95 15.61 11.10 10.13 11.01 0.051 0.074 0.048 0.087 0.085 0.077 Kidnevs 2.67 3.02 3.22 1.98 2.15 2.15 3.65 3.20 3.23 2.26 2.40 2.09 2.80 3.75 2.98 2.30 2.52 2.52 Testes/ Ovaries 3.30 3.29 3.50 0.185 0.248 0.239 3.80 3.55 3.41 0.180 0.192 0.160 3.32 3.40 3.25 0.124 0.160 0.203 Thymus 0.58 0.88 0.90 0.41 0.64 0.51 0.90 0.83 0.80 0.52 0.61 0.60 0.58 0.75 0.68 0.61 0.60 0.65 Heart 1.18 1.41 1.38 0.93 0.90 0.85 1.70 1.41 1.41 0.80 0.91 0.82 1.05 1.60 1.20 0.88 0.92 1.00 Lung Thyroid 2.43 2.23 2.75 1.57 1.50 1.52 0.030 0.034 0.042 0.041 0.018 0.020 2.23 2.30 2.34 2.11 1.42 1.35 0.035 0.034 0.038 0.027 0.023 0.037 1.64 2.09 1.72 1.70 1.32 1.78 0.018 0.044 0.028 0.029 0.034 0.028 Brain 2.21 2.07 1.93 1.80 1.92 1.90 2.15 2.00 2.22 1.98 1.81 2.06 2.01 2.13 1.90 1.78 1.93 1.98 Pituitary 0.010 0.010 0.010 0.011 0.010 0.014 0.014 0.015 0.012 0.015 0.010 0.017 0.012 0.014 0.010 0.009 0.014 0.013 Page 68 Company Sanitized. Does not contain TSCA CBI ,Ninety-Day Feeding Study in the Rat. TABLE 28. Continued. Organ Weights, Grams. - Animal Terminal No. & Weight Spleen Group Sex Grams 60-DAY INTERIM SACRIFICE: Control: 14167 M 14170 M 14178 M 14143 F 14149 F 14152 F 340 385 340 200 260 240 0.81 0.71 0.75 0.49 0.47 0.59 100 ppm. : 14217 M 14220 M 14221 M 14184 F 14193 F 14194 F 1000 ppm,; 14246 M 14251 M 14253 M 14223 F 14230 F 14234 F 325 0.79 340 0.79 350 0.79 215 0.41 235 0.54 240 0.61 320 0.62 360 0.79 385 0.81 215 0.55 220 0.65 195 0.41 5000 ODDI , J 14289 M 14293 M 14295 M 14263 F 14270 F 14275 F 450 355 430 265 200 230 0.71 1.34 0.66 0.50 0.55 0.80 Liver Adrenals 13.13 14.70 11.24 7.28 8.88 8.58 0.058 0.069 0.059 0.059 0.071 0.080 11.78 10.52 10.44 8.29 7.70 0.051 0.053 0.041 0.062 0.064 0.069 11.75 14.00 13.08 8.24 8.01 7.42 0.050 0.048 0.041 0.081 0.076 0.068 26.05 16.75 22.33 13.71 10.29 12.11 0.070 0.067 0.075 0.074 0.063 0.065 Kidnevs 2.60 2.90 2.36 1.55 1.90 1.71 2.69 2.31 2.59 1.61 1.66 1.82 2.57 3.09 2.45 1.89 2.01 1.85 3.49 3.33 3.56 2.46 2.20 2.41 Testes/ Ovaries Thvmus 3.14 2.89 3.19 0.170 0.100 0.130 0.69 0.70 0.52 0.49 0.69 0.53 3.32 3.31 3.27 0.144 0.135 0.118 0.83 0.96 0.59 0.45 0.47 0.58 2.70 3.17 3.37 0.138 0.200 0.149 0.60 0.62 0.77 0.58 0.50 0.60 1.69, 2. 95 1.00 3.32 0.91 3.49 0.78 0.168 0.68 0.079 0.50 0.188 0.50 Heart 1.16 1.48 1.58 0.75 0.98 0.84 1.13 1.22 1.15 0.70 0.80 0.78 1.12 1.11 1.05 0.89 0.88 0.66 1.40 1.38 1.29 0.87 0.80 0.89 Lune Thvroid 1.67 2.03 2.02 1.07 1.22 1.19 0.027 0.027 0.025 0.015 0.031 0.027 1.60 1.81 1.69 1.15 1.78 1.38 0.037 0.025 0.019 0.044 0.023 0.042 1.95 1.81 2.01 1.40 1.62 1.21 0.024 0.029 0.014 0.042 0.038 0.018 1.89 2.19 2.11 1.29 1.44 1.30 0.029 0.036 0.025 0.021 0.033 0.022 Brain 1.81 1.20 1.81 1.87 1.74 1.75 2.01 1.71 1.92 1.79 1.73 1.73 1.91 1.99 2.09 1.82 1.90 1.90 1.90 1.96 2.08 1.80 1.88 1.90 Pituitarv 0.014 0.017 ' 0.011 0.01 0.009 0.014 0.010 0.009 - 0.012 0.013 0.010 0.010 0.012 0.008 0.010 0.009 0.010 0.014 0.013 0.009 0.019 0.012 0.007 Page 69 Company Sanitized. Does not contain TSCA CBI Ninety-Day Feeding Study in the Rat. TABLE 28. Continued Organ Weights, Grams. Animal Terminal No. & Weight Spleen Group Sex Grams 90-DAY TERMINAL SACRIFICE: Control ; 14163 M 14164 M 14166 M 14169 M 14171 M 14172 M 14173 M 14174 M 14175 M 14176 M 14144 F 14147 F 14148 F 14150 F 14151 F 14153 F 14154 F 14155 F 14156 F 14157 F 410 545 420 460 430 490 800 450 545 400 325 255 280 300 250 255 275 255 290 210 1.10 1.22 0.91 1.11 1.32 1.10 0.99 1.00 1.28 0.75 1.00 0.51 0.76 0.75 0.71 0.69 0.72 0.81 0.78 0.39 Liver Adrenals 11.63 17.92 12.83 14.18 13.81 15.47 11.91 15.12 17.71 10.48 9.90 7.11 8.62 9.80 7.50 7.88 8.39 8.32 8.63 5.58 0.061 0.059 0.053 0.063 0.066 0.078 0.058 0.080 0.065 0.081 0.096 0.065 0.060 0.084 0.051 0.080 0.078 0.090 0.090 0.055 Kidneys 2.29 3.71 2.51 3.10 2.79 3.10 2.39 3.02 3.12 2.65 2.49 1.52 1.70 1.92 1.61 1.58 1.72 1.60 1.89 1.23 Testes/ Ovaries 2.80 3.58 3.22 3.65 3.37 3.19 3.15 3.31 3.49 3.42 0.242 0.134 0.155 0.167 0.104 0.147 0.140 0.70 0.154 0.123 Thvmus 0.52 1.09 0.43 0.71 0.59 0.95 0.41 0.69 0.60 0.58 0.60 0.52 0.39 0.51 0.60 0.60 0.51 0.53 0.38 0.41 Heart 1.22 1.90 1.13 1.23 1.40 1.38 1.12 1.38 1.58 1.39 1.05 0.80 0.71 0.98 0.75 0.83 0.78 0.85 0.89 0.73 Lung Thvroid 2.00 2.38 1.64 1.78 2.09 2.07 2.03 1.60 2.92 1.68 1.71 1.00 1.35 1.52 1.21 1.61 1.48 1.37 1.40 1.05 0.040 0.051 0.019 0.036 0.021 0.024 0.020 0.019 0.026 0.025 0.031 0.015 0.024 0.031 0.031 0.023 0.030 0.024 0.023 0.018 Brain Pituitary 1.88 2.02 1.88 2.01 1.81 1.91 1.80 1.85 2.00 2.08 1.85 1.70 1.80 1.92 1.65 1.85 1.82 1.80 1.89 1.89 0.009 0.017 0.014 0.012 0.014 0.013 0.014 0.015 0.015 0.012 0.014 0.013 0.013 0.013 4. 0.014 0.016 0.012 0.008 Company Sanitized. Does not contain T S C A CBI Ninety-Day Feeding Study in the Rat. FABLE 28 . Continued. Organ Weights, Grams. Animal Terminal Sto. & Weight Spleen SrouD Sex Grams 90-DAY TERMINAL SACRIFICE: LOO ppm.: L4203 M 1.4204 M 14206 M 14207 M 14209 M 14210 M 14211 M 14213 M. 14214 M 14215 M 14216 M 14218 M 14219 M 14183 F 14186 F 14187 F 14189 F 14190 F 14191 F 14192 F 14195 F 14196 F 14197 F 14198 F 14199 F 14200 F 14201 F 455 560 465 435 400 520 470 380 485 420 535 520 450 310 345 280 265 255 290 245 280 270 260 295 280 285 310 1.49 0.99 1.20 1.20 1.21 1.24 1.48 1.03 1.02 0.70 0.90 1.41 1.00 0.64 0.81 0.81 1.10 0.70 1.22 0.68 0.71 0.89 0.54 1.00 0.76 0.81 0.68 Liver Adrenals 1.4.08 17.48 14.60 12.02 12.0/ 15.48 13.78 11.20 14.03 12.49 15.90 17.65 13.65 8.78 9.62 8.00 8.40 7.58 7.51 7.58 8.50 8.59 7.32 8.40 8.83 8.80 7.88 0.051 0.061 0.067 0.060 0.054 0.053 0.035 0.051 0.061. 0.056 0.055 0.081 0.055 0.076 0.093 0.074 0.081 0.067 0.061 0.069 0.093 0.064 0.049 0.055 0.050 0.077 0.084 Kidneys 2.50 3.38 3.25 3.05 2.72 2.79 3.10 2.61 2.89 2.80 3.29 3.02 2.95 1.93 2.15 1.77 1.91 1.58 1.96 1.80 2.01 1.80 1.72 1.79 1.81 1.98 1.99 Testes/ Ovaries 3.05 3.70 3.50 3.93 3.19 3.10 3.42 3.43 3.50 3.33 3.32 3.74 2.85 0.210 0.203 0.186 0.183 0.165 0.168 0.161 0.161 0.177 0.116 0.181 0.170 0.119 0.198 Thvmus 0.41 0.60 0.60 0.82 0.44 1.11 0.36 0.58 0.60 0.90 0.59 0.72 0.54 0.50 0.58 0.44 0.81 0.33 0.56 0.30 0.60 0.48 0.49 0.42 0.61 0.71 0.48 Heart 1.43 1.73 1.38 1.22 1.28 1.39 1.43 1.20 1.46 1.30 1.50 1.71 1.40 0.80 1.14 0.79 0.83 0.71 0.92 0.72 0.93 0.89 0.88 0.79 1.01 0.81 0.93 Lung Thyroid 2.28 2.10 2.72 1.74 1.90 2.20 1.95 1.62 2.71 2.08 2.02 2.21 1.90 1.52 1.34 1.21 1.42 1.29 1.43 1.43 1.30 1.53 1.28 1.60 1.41 1.37 1.50 0.019 0.025 0.024 0.023 0.025 0.021 0.039 0.027 0.028 0.036 0.022 0.029 0.028 0.034 0.026 0.027 0.023 0.016 0.023 0.026 0.028 0.022 0.014 0.019 0.023 0.020 Brain 1.85 1.93 2.08 2.08 1.82 1.90 2.19 2.09 2.09 2.00 1.97 1.83 2.08 1.78 2.02 1.90 1.73 1.75 1.89 1.91 1.88 1.92 1.77 1.92 1.81 1.87 2.09 Pituitary 0.007 0.014 0.011 0.009 0.009 0.010 0.011 0.009 0.013 0.011 0.013 0.015 0.010 0.014 0.014 0.018 0.015 0.011 0.012 0.011 0.014 0.008 0.012 0.013 0.015 0.014 0.013 Page 71 Company Sanitized. Does not contain TSCA CBI 'mm9\ Ninety-Day Feeding Study in the Rat ''TABLE 28. Continued. Organ Weights, Grams. Animal No. & Group Terminal Weight Sex Grams , Spleen Liver Adrenals Kidneys Testes/ Ovaries Thymus Heart Lung Thyroid Brain Pituitary 90-DAY TERMINAL SACRIFICE: 1000 ppm.: 14243 M 14244 M. 14247 M 14249 M 14250 M 14252 M 14254 M 14255 M L4256 M 14257 M 14224 F 14226 F 14227 F 14229 F 14231 F 14232 F 14233 F 14235 F 14236 F 14237 F 455 535 475 470 440 470 460 410 385 415 290 270 295 305 320 280 230 325 275 250 1.09 0.97 0.99 1.17 0.89 0.79 1.18 1.00 1.01 0.63 0.69 0.51 0.50 0.89 0.77 0.77 1.22 0.84 0.61 0.88 12.68 17.13 16.20 15.88 16.13 16.61 15.50 12.80 12.39 14.90 12.38 8.91 9.40 10.75 11.55 11.00 14.39 8.14 10.52 8.81 0.054 0.065 0.053 0.082 0.057 0.067 0.058 0.053 0.055 0.055 0.107 0.066 0.073 0.075 0.097 0.096 0.116 Missed 0.074 0.077 2.87 3.51 3.00 3.21 3.01 3.42 3.23 2.60 2.58 3.03 2.60 2.21 2.49 2.10 2.55 2.17 2.89 1.73 2.21 2.08 3.39 3.60 3.40 3.58 3.30 3.80 3.41 3.30 2.81 3.29 0.172 0.097 0.127 0.186 0.206 0.201 0.171 0.130 0.144 0.117 0.27 0.98 0.41 0.68 0.42 0.60 0.59 0.79 0.35 0.78 0.40 0.22 0.35 0.61 0.37 0.80 0.68 0.31 0.50 0.75 1.30 1.50 1.31 1.51 1.32 1.39 1.31 1.21 1.10 1.21 0.90 1.40 0.85 0.98 1.09 0.88 1.12 0.70 0.91 1.00 1.92 2.94 2.21 1.65 1.98 1.93 2.75 2.00 1.71 1.68 1.49 1.39 1.52 1.35 1.83 1.24 1.65 1.20 1.33 1.79 0.019 0.026 0.031 0.034 0.031 0.029 0.017 0.026 0.024 0.040 0.027 0.037 Missed Missed 0.026 0.040 0.031 0.022 0.028 0.034 1.82 1.98 2.25 1.93 1.91 1.89 1.81 2.01 1.98 1.99 1.81 1.89 1.90 1.91 2.08 1.90 1.99 1.68 1.92 1.85 0.030 0.012 0.010 0.012 0.013 Missed 0.012 0.012 0.008 0.009 0.011 0.005 0.009 0.012 0.010 0.014 0.019 0.014 0.017 0.016 Page 72 Company Sanitized. Does not contain T S C A CBI Ninety-Day Feeding Study in the Rat. `TABLE 28. Continued. Organ Weights , Grams. 7 Animal Terminal No. & Weight Spleen Group Sex Grams 90-DAY TERMINAL SACRIFICE: 1.000 D D m . : 14293 M 14244 M 14247 M 14249 M 142.50 M 14252 M 14254 M 14235 M 1.4256 M 14257 M 14224 F 14226 F 14227 F 14229 F 14231 F 14232 F 14233 F 14235 F 14236 F 14237 F 455 535 4 75 470 440 470 460 410 .385 415 290 270 295 305 .320 280 230 325 275 250 1.09 0.97 0.99 1.17 0.89 0.79 1.18 1.00 1.01 0.63 0.69 0.51 0.50 0.89 0.77 0.77 1.22 0.84 0.61 0.88 Liver Adrenals 12.68 17.13 16.20 15.88 16.13 16.61 15.50 12.80 12 .'39 14.90 12.38 8.91 9.40 10.75 11.55 11.00 14.39 8.14 10.52 8.81 0.054 0.065 0.053 0.082 0.057 0.067 0.058 0.053 0.055 0.055 0.107 0.066 0.073 0.075 0.097 0.096 0.116 Missed 0.074 0.077 Kidneys 2.87 3.51 3.00 3.21 3.01 3.42 3.23 2.60 2.58 3.03 2.60 2.21 2.49 2.10 2.55 2.17 2.89 1.73 2.21 2.08 Testes/ Ovaries 3.39 3.60 3.40 3.58 3.30 3.80 3.41 3.30 2.81 3.29 0.172 0.097 0.127 0.186 0.206 0.201 0.171 0.130 0.144 0.117 Thvmus 0.27 0.98 0.41 0.68 0.42 0.60 0.59 0.79 0.35 0.78 0.40 0.22 0.35 0.61 0.37 .0.80 0.68 0.31 0.50 0.75 Heart 1.30 1.50 1.31 1.51 1.32 1.39 1.31 1.21 1.10 1.21 0.90 1.40 0.85 0.98 1.09 0.88 1.12 0.70 0.91 1.00 Lung Thvroid Brain 1.92 2.94 2.21 1.65 1.98 1.93 2.75 2.00 1.71 1.68 1.49 1.39 1.52 1.35 1.83 1.24 1.65 1.20 1.33 1.79 0.019 0.026 0.031 0.034 0.031 0.029 0.017 0.026 0.024 0.040 0.027 0.037 Missed Missed 0.026 0.040 0.031 0.022 0.028 0.034 1.82 1.98 2.25 1.93 1.91 1.89 1.81 2.01 1.98 1.99 1.81 1.89 1.90 1.91 2.08 1.90 1.99 1.68 1.92 1.85 Pituitary 0.030 0.012 0.010 0.012 0.013 Missed 0.012 0.012 0.008 0.009 0 .011 0.005 0.009 0.012 0.010 0.014 0.019 0.014 0.017 0.016 Page 72 Company Sanitized. Does not contain T S C A CBI Ninety-Day Feeding Study in the Rat. TABLE 28. Continued. Organ Weights , Grams. 4 Animal Terminal No. & Weight Spleen Group Sex Grams 90-DAY TERMINAL SACRIFICE: 5000 d o d i . : 14283 M 14284 M 14286 M 14287 M 14290 M 14291 M 14292 M 14294 M 14296 M 14297 M 14264 F 14266 F 14267 F 14269 F 14271 F 14272 F 14273 F 14274 F 14276 F 14277 F 420 525 345 425 430 410 390 395 430 480 220 225 250 220 270 215 240 240 250 235 1.30 1.02 0.98 1.11 0.90 0.51 1.19 0.77 1.12 0.91 0.90 0.83 0.48 0.58 1.08 0.67 0.70 1.51 0.48 0.83 Liver Adrenals 19.57 0.058 25.68 0.065 15.13 0.059 20.11 0.050 21.49 0.059 21.65 0.060 19.82 0.050 18.30 0.054 19.82 0.054 22.19 0.058 9.84 0.063 11.40 0.077 10.85 0.067 11.01 0.068 11.91 0.072 9.57 0.083 11.75 0.062 10.41 . 0.059 11.30 0.085 11.41 0.079 Kidnevs 3.30 3.91 2.62 3.30 3.38 3.18 3.20 3.30 3.31 3.80 2.00 2.03 2.28 2.25 2.78 1.80 2.38 2.45 2.12 2.40 Testes/ Ovaries 3.30 2.87 3.21 3.39 3.60 3.20 3.49 3.40 3.60 3.61 0.146 0.122 0.178 0.092 0.130 0.180 0.107 0.144 0.182 Thvmus 0.58 1.32 0.69 0.80 0.42 0.59 0.63 0.30 0.79 0.51 0.41 0.58 0.53 0.57 0.58 0.42 0.51 0.43 0.48 Heart 1.21 1.43 0.91 1.30 1.58 1.17 1.20 1.38 1.42 1.28 0.78 0.80 0.72 0.83 0.98 0.70 0.90 0.83 1.03 0.95 Lung Thyro id 1.88 2.35 2.18 1.98 1.61 2.02 1.42 2.09 1.60 1.92 1.38 2.93 1.28 1.13 1.40 1.32 1.21 1.60 1.61 1.51 0.024 0.035 0.023 0.029 0.027 0.031 0.019 0.021 0.023 0.017 0.020 0.018 0.019 0.024 0.018 0.032 0.018 0.015 0.031 0.025 Brain 2.04 2.09 2.08 2.10 1.92 1.83 2.02 1.90 1.92 2.05 1.78 1.88 1.80 1.91 1.79 1.91 1.98 1.81 1.81 1.78 Pituitary 0.010 0.015 ' 0.008 0.011 0.012 0.010 0.009 0.008 0.010 0.011 0.010 0.011 0.012 0.011 . 0.010 0.007 0.008 0.013 Page 73 Company Sanitized. Does not contain TSCA CBI :Page 74 Ninety-Day Feeding Study in the Rat. TABLE 29. Incidence of Histopathologic Lesions. brain - no lesion - glial nodules spinal cord - no lesion peripheral nerve - no lesion eye - no lesion pituitary - no lesion thyroid - no lesion parathyroid - no lesion adrenal - no lesion - ossification lung - no lesion - chronic respiratory disease heart - no lesion - arteritis spleen - no lesion - hematopoiesis lymph node - no lesion thymus - no lesion bone marrow - no lesion saliv'ary gland - no lesion stomach - no lesion small intestine - no lesion large intestine - no lesion - nematodes 30 Day Interim Sacrifice 60 Day Interim Sacrifice Control 2500 ppm. Control 5000 ppm. M FM FM FMF 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 2/2 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 2/2 2/2 3/3 2/2 1/1 3/3 2/2 3/3 2/2 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 1/1 3/3 3/3 2/2 1/1 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 2/3 3/3 2/3 3/3 2/3 3/3 3/3 1/3 1/3 1/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 2/3 3/3 3/3 3/3 3/3 3/3 1/3 3/3 3/3 3/3 3/3 2/2 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 2/2 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 2/3 3/3 1/3 _ Company Sanitized. Does not contain TS CA CB 90 Day Tieerminal Sacrifice_______ Control 1000 Dom. 5000 DDm. __M__ F__ _M__ __E-- ___ n---_ J L ------- 10/10 10/10 9/10 10/10 21 Day ___ Withdrawal. Control 5000 DDm, M T? M F 3/3 3/3 3/3 3/3 1/10 8/8 9/9 9/9 9/9 10/10 9/9 9/9 3/3 10/10 9/9 : 5/5 6/6 10/10 10/10 9/9 9/9 9/9 8/8 9/9 9/9 8/8 1/1 10/10 9/9 3/3 2/2 9/10 10/10 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 1/1 1/1 3/3 1/1 3/3 3/3 3/3 3/3 j 5/10 9/10 5/10 1/10 9/10 10/10 1/10 7/10 7/10 3/10 3/10 10/10 10/10 2/3 1/3 2/3 1/3 1/3 2/3 1/3 2/3 3/3 3/3 3/3 3/3 1/10 10/10 10/10 9/9 10/10 3/3 3/3 3/3 3/3 9/9 9/9 10/10 9/9 10/10 10/10 10/10 8/8 9/9 10/10 9/9 10/10 10/10 10/10 8/8 10/10 9/9 8/8 9/9 10/10 9/9 ' 8/8 10/10 10/10 10/10 10/10 10/10 9/10 1/10 3/3 2/2 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 Company Sanitized. Does not contain TSCA CBI Page 75  j!.age 76 Ninety-Day Feeding Study in the Rat. ^ ---- Stable 2 9 . continued. Incidence of Histopathologic Lesions. 30 Day 60 Day Interim Sacrifice Interim Sacrifice Control 2500 ppm. Control 5000 ppm. Tissue and Lesion mfmfmfmf pancreas - no lesion 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 pancreatitis, focal liver - no lesion - portal inflammatory infiltrate - centrolobular change kidney - no lesion - focal nephritis 2/3 3/3 0/3 2/3 2/3 2/3 0/3 0/3 1/3 1/3 1/3 1/3 1/3 1/3 3/3 3/3 2/2 3/3 3/3 3/3 2/3 3/3 3/3 3/3 3/3 1/3 - hyaline droplets urinary bladder - no lesion - siminal plug W . .. testes or ovaries - no lesion - edema prostate or uterus - no lesion 2/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 1/3 3/3 3/3 3/3 3/3 3/3 3/3 2/3 3/3 1/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 - hydrometra skeletal muscle - no lesion skin - no lesion bone - no lesion 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 Company Sanitized. Does not contain TSCA CBI 90 Day Terumiij.nuaali Sacrifice_____ 11*G 1 Control 1000 DDm. 5000 M T? M F M F 10/10 10/10 10/10 10/10 7/10 3/10 1/10 8/10 5/10 5/10 10/10 7/10 3/10 6/10 4/10 1/10 1/10 9/10 10/10 2/10 8/10 2/10 9/10 1/10 2/10 6/8 9/9 k}2/8 ^!o/io 10/10 10/10 9/9 10/10 10/10 10/10 9/10 1/10 9/9 10/10 9/9 10/10 ; L0/10 10/10 10/10 10/10 10/10 9/9 9/9 6/6 10/10 10/10 21 Day Withdrawal Control 5000 DDm, M FM F 2/3 3/3 3/3 3/3 1/3 2/3 2/3 0/3 0/3 1/3 1/3 1/3 3/3 2/3 3/3 3/3 3/3 2/3 1/3 1/2 3/3 3/3 3/3 1/2 3/3 3/3 3/3 3/3 3/3 3/3 2/2 2/2 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 3/3 Page 77 Company Sanitized. Does not contain TSCA CBI Page 78 *-- V TABLE 3Q. Ninety-Day Feeding Study in the Rat Histopathologic Observations. Thirty-Day Interim Sacrifice. Animal Number Sex Tissue Comment Control : 14162 14165 14168 14142 14145 14146 M M M F F F 2500 DDm.: 14282 14285 M M 14288 M 14262 14265 14268 F F F liver urinary bladder lung Mild portal lymphocytic infiltrate. No lesion. Seminal plug. No lesion ... Small focus of pneumonic consolidation. No lesion. liver liver liver spleen liver kidney lung Slight hypertrophy of centrolobular hepatocytes. Slight hypertrophy of centrolobular hepatocytes with loss of coarse cytoplasmic granularity. Slight hypertrophy of centrolobular hepatocytes with loss of usual coarse granularity. Moderate hematopotic activity. 1 Mild portal lymphocytic infiltrate. Mild focal interstitial lymphocytic infiltrate. Moderate peribronchial lymphoid hyperplasia. No lesion. * _______ Company Sanitized. Does not contain TSCA CBI Page 79 Ninety-Day Feeding Study in the Rat. TABLE 30. Continued. Histopathologic Observations. Sixty-Day Interim Sacrifice. Animal Number Sex Tissue Comment Control: 14167 14170 14178 14143 14149 14152 5000 DDm. 14289 M M M F F F M 14293 M 14295 14263 14270 14275 M F F F liver liver lung No lesion. No lesion. Slight portal lymphocytic infiltrate. No lesion. Slight portal lymphocytic infiltrate. _ Few small scattered foci of pneumonic consolidation. No lesion. large intestine testes liver liver liver liver liver liver Nematodes. One testis was edematous and had reduced spermatogenic activity. Hypertrophy of hepatocytes which was more pronounced in the centrolobular area; hepatocytes appeared to have higher glycogen content than controls. Moderate hypertrophy of hepatocytes,. predominately centrolobular with loss of coarse granularity. Moderate portal lymphocytic infiltrate with scattered small nodules of proliferated reticuloendothelial cells in liver parenchyma. Hypertrophy of hepatocytes, primarily centrolobular, hepatocytes appeared to contain more glycogen than control. Slight hypertrophy of hepatocytes, predominately centrolobular. Slight portal lymphocytic infiltrate. Slight hypertrophy of centrolobular hepatocytes. Company Sanitized. Does not contain TSCA CBI Page 80 Ninety-Day Feeding Study in the Rat. TABLE 39. Continued. Histopathologic Observations. Ninety-Day Terminal Sacrifice. Animal Number Sex Tissue Comment Control : 14163 14164 14166 14169 M M M M 14171 14172 14173 14174 14175 M M M M M 14176 14144 14147 14148 M F F F lung urinary bladder kidney liver lung urinary bladder lung lung kidney liver lung liver heart liver Moderate perivascular lymphocytic cuffing. Seminal plug. No lesion. Small numbers of hyaline droplets in epithelium of convoluted tubules. Slight portal lymphocytic infiltrate and bile duct proliferation. , Moderate perivascular lymphocytic cuffing with localized pneumonitis. Seminal plug. Slight perivascular lymphocytic cuffing, peribronchial lymphoid hyperplasia. Slight peribronchial lymphoid hyperplasia. Moderate numbers of hyaline droplets in epithelium of convoluted tubules. No lesion. Slight portal lymphocytic infiltrate, centrolobular hepatocytes less coarsely granular. Slight perivascular lymphocytic cuffing. Slight portal lymphocytic infiltrate. Moderate mural necrosis and perivascular inflammatory infiltrate of a coronary vessel. No lesion. No lesion. Slight portal lymphocytic infiltrate and bile duct proliferation. Company Dpgg notaonialn TSCJ\ Page 81 Ninety-Day Feeding Study in the Rat. TABLE 30. Continued. Histopathologic Observations. Ninety-Day Terminal Sacrifice. Animal Number Sex Tissue Comment Control (cont'd): 14150 14151 14153 F F liver F lung liver 14154 F uterus liver 14155 F liver 14156 F 14157 F No lesion. Slight portal lymphocytic infiltrate. Small area of chronic pneumonitis, Slight portal lymphocytic infiltrate. Hydometra. Slight portal lymphocytic infiltrate. Slight portal lymphocytic infiltrate. No lesion. No lesion. ' - Con,pany Sanitized. Doesnot contain Tsca CBl Page 82 Ninety-Day Feeding Study in the Rat. TABLE 30. Continued. Histopathologic Observations . Ninety-Day Terminal Sacrifice. Animal Number Sex Tissue Comment 1000 D D m . ,. Liver Only: 14243 M. 14244 M 14247 M 14249 M 14250 M 14252 M 14254 M 14255 M 14256 M 14257 M 14224 F 14226 F 14227 F 14229 F 14231 F 14232 F 14233 F 14235 F 14236 .F 14237 F No lesion. No lesion. Slight portal lymphocytic infiltrate. No lesion. No lesion. No lesion. No lesion. No lesion. Slight portal lymphocytic infiltrate. Slight portal lymphocytic infiltrate. No lesion. No lesion. No lesion. Slight portal lymphocytic infiltrate. No lesion. Slight portal lymphocytic infiltrate. Slight portal lymphocytic infiltrate. Slight portal lymphocytic infiltrate. No lesion.No lesion. - &ompan Sanitized. Does not contain 73GAS^ Page 83 Ninety-Day Feeding Study in the Rat. TABLE 30. Continued. Histopathologic Observations. Ninety-Day Terminal Sacrifice. Animal Number Sex Tissue Comment 5000 ppm.: 14283 M liver 14284 14286 M liver lung M brain lung liver 14287 M adrenal... liver 14290 14291 14292 14294 14296 M M liver M liver lung M liver M liver Centro lobular hepatocytes slightly hypertrophied cytoplasm less coarsely granular than in hepatocytes at periphery of lobules. Centrolobular hepatocytes less coarsely granular than those at periphery. Slight peribronchial lymphoid hyperplasia. Glial nodules in medulla, structure resembling Sarcosporidia also present. Slight perivascular lymphocytic cuffing, area of pneumonic consolidation. Centrolobular hepatocytes less granular than those at periphery of lobule, slight portal lymphocytic infiltrate. Area of osteoid and bone in cortex of one adrenal. Cytoplasm of centrolobular hepatocytes less coarsely granular than cytoplasm of hepatocytes at periphery, slight portal lymphocytic infiltrate. No lesion. Cytoplasm of centrolobular hepatocytes less coarsely granular than cytoplasm of hepatocytes at periphery of lobules. Centrolobular hepatocytes less coarsely granular than those at periphery of lobules. Slight peribronchial lymphoid hyperplasia. _ Centrolobular hepatocytes less coarsely granular than those at periphery of lobule. Marked portal lymphocytic infiltrate, centrolobular hepatocytes slightly less granular than those at periphery of lobule. OJWpanySanHizeti. Does no! conu*, T8CA cbi Page 84 Ninety-Day Feeding Study in the Rat. TABLE 30. Continued. Histopathologic Observations. Ninety-Day Terminal Sacrifice. Animal Number Sex Tissue Comment 5000 D D m . Ccont'd) m 14297 M liver 14264 14266 F liver F liver 14267 14269 14271 14272 14273 14274 lung F liver F liver F kidney liver F liver F liver large intestine F liver 14276 14277 F lung F lung Centrolobular hepatocytes less coarsely granular than those at periphery of lobule. Moderate portal lymphocytic infiltrate. Centrolobular hepatocytes less coarsely granular than those at periphery, mild portal lymphocytic infiltrate. Chronic murine pneumonia of moderate severity. Mild portal lymphocytic infiltrate. Slight portal lymphocytic infiltrate. Moderate interstitial lymphocytic infiltrate. . Mild portal lymphocytic infiltrate. Mild portal lymphocytic infiltrate. Mild portal lymphocytic infiltrate. Nematodes. Mild portal lymphocytic infiltrate, centrolobular hepatocytes less coarsely granular than those at periphery. Moderate perivascular lymphocytic cuffing. Moderate perivascular lymphocytic cuffing. Company Sanitized, Does not contain ISCA CBJ Page 85 Ninety-Day Feeding Study in the Rat. TABLE 3Q. Continued. Histopathologic Observations. 21-Day Compound Withdrawal. Animal Number Sex Tissue Comment Control: 14177 14179 1418 M M M 14158 14159 14161 F F F 5000 ppm.: 14298 M 14299 M 14300 14278 M F 14279 14280 F F pancreas lung liver urinary bladder lung liver lung No lesion. Small area of necrosis and chronic inflammation. Moderate peribronchial lymphoid hyperplasia. Slight portal lymphocytic infiltrate. Seminal plug. No lesion. Moderate peribronchial lymphoid hyperplasia. Slight portal lymphocytic infiltrate. Slight perivascular lymphocytic cuffing. liver lung urinary bladder liver liver kidney liver lung liver lung liver Slight portal lymphocytic infiltrate. Mild peribronchial lymphoid hyperplasia. Seminal plug. Centrolobular hepatocytes appeared slightly hypertrophied. Centrolobular hepatocytes appeared slightly hypertrophied. Slight interstitial lymphocytic infiltrate, few calcified tubules. Moderate lymphocytic inflammatory infiltrate, primarily in portal areas. Moderate peribronchial lymphoid hyperplasia. Mild portal lymphocytic infiltrate, few scattered vacuolated hepatocytes. Slight peribronchial lymphoid hyperplasia. Slight portal lymphocytic infiltrate. Company Sanitized. Does not contain TSCA CBi