Document 2NdanOjoL5zqd08zwvJLONJVr

ACUTE TOXICITY TO INVERTEBRATES TEST SUBSTANCE (GRASS SHRIMP) Identity:A mixturecontainingperfluorooctanesulfonatweh,ich may alsobe referredtoas PFOS, FC-95, oras a component ofFC-206 or L3243. (1-Octanesulfoniacid)(CAS # 2795-39-3). Remarks: The 3M productionlotnumber was not noted.The testsample isFC-206, identifiebdy the laboratoryas "Sample C". Currentinformation indicatesitisa mixtureof0.67% PFOS, 17.5% diethyleneglycolbutylether, 78.91% water,1.33% Sultonefoamer, 1% sodium octylsulfate,0.04% sodium laurylsulfate0,.5% polyoxyethylenemonooctylphenylether,and 0.05% benzotriazole. The followingsummary applies to a mixture with incompletely characterized concentrations of impurities.Data may not accurately reflecttoxicityof the fluorochemical component of the testsample. METHOD: Method: Standard Methods (APHA) 1971 Edition Type: Acute static GLP: No Year completed: 1974 Species: Palaemonetes vulgaris Supplier:FieldcollecteldocalliynMA. Analyticalmonitoring: Temperature,and dissolvedoxygen. Exposure period: 96-hours Statisticamlethods: TL50(mediantolerancelimitv)aluescalculateudsinga linearregressionequation. Test organism age: Juveniles. Length and weight: Average length= 30 mm Loading: Not given. Pretreatment: None Test conditions: Dilutionwater: Syntheticsea water Dilutionwater chemistry: pH 8.0 Salinity 25 ppt Lighting: Not given. Stock and testsolutionpreparation: Not given. Concentrations dosing rate:Once Stabilityof the testchemical solutions: Not noted Exposure vessels: 5 gallonglassvesselscontaining15 L oftestsolution. Number of replicates:1 Number oforganismsperreplicate1:0 Number ofconcentrations:ixplusa blankcontrol Water chemistryduringthestudy: Temperaturerange(0-96hours):20 1 OC Dissolvedoxygenrange(0-96hours):4.2- 8.6mg/L RESULTS Nominal concentrations: Blank control,100, 180,240, 320, 420, and 1000 mg/L Element values: 96-hour TL50 = 280 (230 - 342) mg/L 96-hour NOEL = 180 mg/L Element values based on nominal concentrations. Remarks: Testingwas conducted on the mixtureas describedinthe Test Substance Remarks field.The values reportedapplytothatmixtureand not the fluorochemicalproportionalone. CONCLUSIONS The testsample 96-hourLC.50forgrassshrimp was determined to be 280 mg/L witha 95% confidenceintervaolf230 to 342 mg/L. The 96-hour no observed effectlevel(NOEL) was 180 mg/L. Submitter: 3M Company, Environmer,.tLaalboratory,P.O. Box 33331, St. Paul,Minnesota,55133 DATA QUALITY ReliabilitKyl:imischranking2. Testingmeets the criterifaorqualitytesting. However, the sample puritywas notproperlycharacterizedand the study lacksanalyticaclonfirmatioonfthe amount offluorochemicalproportioninthe solution. REFERENCES Test was conducted by Bionomics,EG&G, Inc.o,fWareham, MA atthe requestofthe 3M Company, St.Paul,MN, 1974. OTHER Last changed: 6/28/00 BIOASSAY REPORT SUBMITTED TO 3,M COMPANY SAINT PAUL, MINNESOTA 0-ct ACUTE TOXICITY OF SAMPLE C TO M,UMMICH(5-G (Fundulus heteroclitu2), GRASS SHRIMP (Palaemonetes vulgaris), AND FIDDLER CRAB (Uca pugilator). Bionom"ics E G & G, Inc. 790 Main Street Wareham, Massachusetts May, 1974 METHODS AND MATERIALS These investiga.tions-were performed at the aquatic toxicology laboratory of,Bionomics, E G & G, Inc., Wareham, Massachusetts. The susceptibility of mummichog (Fundulus heteroclitus), grass shrimp (Palaemonetes vulgaris), and fiddler crab (Uca pugilator) to Sample C,.a brown liquid, tested as 100% active, was evaluated under, static bioassay conditions for a 96 hour period. The results were reported as the medi4n tolerance limit (TL 50)1 the concentration of the sample in. water causing 50 percent mortality after 24 and 96 hours. The predicted TL50 value and its 95% confidence intervals were arrived at by converting the concentrations tested and the corresponding observed percent mortalities- td logs and probits, respectively. These values were then used to calculate a linear regression equation. Test procedures for the static bioassays are those described for Fish Bioassay Procedures in the 1971 edition of'Standard Methods (APHA). The murmichog, grass shrimp, and fiddler crabs used in these tests were collected locally by laboratory personnel. The mummichog had a mean weight and length of 1.1 g and 35 mm,respectively. The grass shrimp had a mean length of 30 mm. width of 20 mm. The fi ddler crabs had a me<E]!E@D Page two The test species were dbserved in the laboratory hatchery facility for at least 30 days prior to testing. These species were acclimated over a 10 day period,prior to testing,to laboratory test diluent water quality and temperature. During that period mortality in the test population was..--'3a%nd these' animals were judged to be in excellent physical condition. .-Thebi6assays were conducted in 5 gallon glass vessels containing 15 1 of water at 200C ( 1.0), and there was a single introduction of the sample into each test vessel. aerated. The test containers were not The test diluent consisted of synthetic sea water I with a salinity of 25 .0/oo, a pH of. 8,0 and a temperature 0 of 20 C ( 1.0). Dissolved oxygen levels for the tests ranged from $.6 at the beginning of the tests to 4.2 at the end of testing. Ten specimens were introduced into each test vessel 24 hours prior to the start of the assay. RESULTS The predicted TL 50 values and 95% confidence intervals are presented in Table 1. Table 2 presents a summary 1 Laroche, G. E. Eisler', and C. R. Tarzwell, 1970. Bioassay Procedures for Oil and Oil Dispersant Toxicity Evaluations. J. W. Poll. Cont. Fed. 42 (11): 1982-1989. Page three of observed mortality for each individual concentration after 24 and 96 hours of exposure, The mortality syndrome in all tests'x4as similar. The mummichog and grass shrimp generally became dark and lethargic, lost equilibrium, and expired. The fiddler crabs became lethargic before expiring, SUBMITTED BY PREPARED BY: APPROVED BY: Bionomics E G & G, Inc. .790 Main Street Wareham, Massachusetts May, 1974 Robert E. Bentley Aquatic Biologist Bevier Hasbrouck Sleight, A Director, Freshwater Programs Table 1 Acute toxicity of Sample C to mummichoga (Fundulus heteroclitus), grass shrimp b (Palaemonetes vulgaris), and fiddler crabc (Uca pugilator).' These data are based on results of bioassays conducted at the fish toxicology of Bionomics, E G & G, Inc., Wareham, Massachusetts. Species munmichog' grass shrimp fiddler crab TL 50 - milligram/liter 24 hour 96- hour @>1,800 <2,400 1,820(1,360-2,420) d No Effect Level (mg/1) 1,400 1, 000'..0 ,/i8O'(230-342) 180 > 10,000 3,260(2,760-3,860) 2,400 aAssays conducted at 200C b Assays conducted at 200C 30 mm. cAssays conducted at 200 C crabs 20 mm. d 95% confidence intervals. 1.0), mean weight of the mummichog 1.1 1.0), mean length of the grass shrimp 1.0).,mean carapace width'of the fiddl( Table 2 Concentrations tested and corresponding observed percent.mortalities of mummichog (Fundulus heteroclitus), grass shrimp (Palaemonetes vul,,aris), and fiddler crab (Ilcapugilator) after 24 and 96 hours of exposure to Sample C. Species mummichog grass shrimp Concentration .(Mg/1). 3,200 2,400 11800 1,400 1,000 control 1,000 420 3-20240 180 140 control % mortality observed 2. Tour vb tiour 100 100 100' 100 0 30 0 0 0 0 0 0' 0 100 0 100 0 100 0 40 0* 20 0 0 0 0 Table 2 Continued Species fiddler crab Concentration (mg/1) 10,000 6,500 4p9oo 3,700 2,800 2,400 2,100 control % morta lity observed 24 hour- 100 100 0 100 0 100 0 70 0 30 0 0 0 0 0 0