Document 0qyz7OOZKDOO0joK9gRr1VRGn

BACK TO MAIN BIODEGRADATION (Pure Microbial Cultures) TEST SUBSTANCE________________ Identity: Perfluorooctanesulfonate; may also be referred to as PFOS or FC-95. (1-Octanesulfonic acid, 1,1,2,2,3,3,4,4,5,5,6,6,7,7,8,8,8heptadecafluoro-, potassium salt, CAS # 2795-39-3) Remarks: The 3M production lot number was 217 (TN-A-2130). The test substance is a white powder. Purity determined to be 86.9% by LC/MS, 1H-HMR, 19F-NMR and elemental analyses techniques subsequent to analyses cited in the attached report. METHOD Method: Springborn Laboratories, Inc./Betts et al, 1974, two study types: Pure Culture Closed Vial Headspace Test Type: Aerobic GLP: No Year completed: 2000 Pure Culture Studies Contact time: 7-days Inoculum: 4 separate pure cultures tested: Cunninghamella echinulata var. echinulata (fungi, ATCC #9244) Mucor circinelloides f. griseocyanus (fungi, ATCC #1207a) Phanerochaete chrysosporium (fungi, ATCC #24725) Streptomyces griseus (actinomycete, ATCC #13273) Source: American Type Culture Collection (ATCC) Inoculum loading in test vessels: 6 mL of Stage II cultures into 60 mL media, allowed 24-hr of agitation prior to PFOS addition Test medium: soybean grits-glucose (SGG) Test concentrations: ~20.9 mg/L (nominal) Study design: Inoculum control: media, inoculum Test: media, inoculum, PFOS stock solution Incubation conditions Temperature: 26C Agitation: Shaker table at 250 rpm Test vessels: Not noted Dosing procedure: Each test flask received all necessary components at test initiation. All work utilized strict aseptic technique until harvest at Day 7. BACK TO MAIN Sampling frequency: Day 0 and 7 Analytical method: Separation of broth and cells via centrifugation. Analysis of both by LC/MS Closed Vial Headspace Study: Contact time: 3-days Inoculum: Phanerochaete chrysosporium (fungi) Inoculum source: American Type Culture Collection (ATCC) Initial inoculum loading in test vessels: not noted Test medium: soybean grits-glucose (SGG) medium at 1/10 and 1/100 strength plus resazurin Test concentration: 0.2 mg/L. Study design: Inoculum control: media, inoculum Abiotic control: media, PFOS (not sterile) Test: media, PFOS, inoculum Incubation conditions Temperature: 26C Agitation: Shaker table, in environmental chamber, at 250 rpm Test vessels: 22 mL sterile vials Dosing procedure: Each test flask received all necessary components at test Initiation. Headspace purged with oxygen, vial immediately crimped. Sampling frequency: Once - Day 3 Analytical method: Separation of broth and cells via centrifugation. Analysis of both by LC/MS Remarks: Stock solutions used to dose the biodegradation test systems were prepared at a concentration of 1,060 mg/L (Pure Culture Study) and 1,011 mg/L (Closed Vial Study). These concentrations are approximately twice the water solubility of PFOS, RESULTS_______________________________________________________ The studies with Cunninghamella, Mucor, and Streptomyces did not provide any indication of biotransformation of PFOS. Preliminary analytical results of the first Phanaerochaete study indicated possible biotransformation of PFOS (90% mass balance). Therefore, closed vial studies were set up to confirm. Difficulties were encountered in maintaining aerobicity, and only 3 days of exposure were maintained. The results from the closed vial study indicated no significant biotransformation of PFOS by Phanaerochaete fungi. BACK TO MAIN CONCLUSIONS It does not appear that the four species studied are capable of metabolizing PFOS. DATA QUALITY Reliability: Klimisch ranking = 2. The stock solution used to dose the systems was prepared at twice the water solubility. There were no initial measured concentrations taken in the closed vial study. These studies were not conducted in accordance with the principles of Good Laboratory Practices. REFERENCES This study was conducted at Springborn Laboratories, Inc., Wareham, Massachusetts at the request of the 3M Company. Report completed 11/3/00. Lab Project number E01-0434. OTHER Last changed: 6/12/01