Document 0q58Q95eeQbDDr4q39J2awqKV

HA ZUEtO IM LABORATORIES AM ERICA, INC. Chemical & BioMedical Sciences Division 3301 KINSMAN BLVD. P.O.BOX 7545 MADISON, WISCONSIN 53707 PHONE (608) 241-4471 TLX 703956 HAZRAL MDS UD FINAL REPORT J ANINE GLEASON MINNESOTA MINING & MANUFACTURING COMPANY TOXICOLOGY SERUICES ST. PAUL, MN 55101 SAMPLE: T-3752 PURCHASE ORDER NUMBER: T357842, REL. #513 \VA. SAMPLE NUMBER: 50503501 SAMPLE ENTERED: 05/15/85 REPORT PRINTED: 06/21/85 ENCLOSED: PRIMARY EYE IRRITATION - METHOD, SUMMARY QAU REPORT RAW DATA APPENDIX SIGNED: & 1 ..L STEUEN M. GLAZA O STUDY DIRECTOR ACUTE TOXICOLOGY ..................... DATE BY AND FOR HAZLETON LABORATORIES AMERICA, INC. RAW DATA FOR THIS STUDY ARE KEPT ON FILE AT HAZLETON LABORATORIES AMERICA, INC., MADISON, WISCONSIN. 001078 HA ZLStO fM LABORATORIES AMERICA. INC. Chemical & BioMedical Sciences Division 3301 KINSMAN BLVD. P.0. BOX 7545 MADISON, WISCONSIN 53707 PHONE (608) 241-4471 TLX 703956 HAZRAL MDS UD SAMPLE NUMBER: 50503501 SAMPLE: T-3752 PAGE 2 OECD EYE IRRITATION Objective: To determine the level of ocular irritation produced following a single exposure of a test substance to one eye of albino rabbits according to the Organisation for Economic Cooperation and Development's Guidelines for Testing Chemicals, Section 405, Acute Eye Irritation/ Corrosion, adopted May 12, 1981. Test Material: T-3752 Physical Description: Purity and Stability: Brown granular solid Sponsor has purity and stability determinations on f i1e . Test animal: Young adult rabbits (approximately 14 weeks of age) of the New Zealand White strain were procured, maintained individually in screenbottom cages in temperature- and humidity-controlled quarters, provided continuous access to Purina High Fiber Rabbit Chow and water, and held for an acclimation period of at least 7 days. Three acclimated animals, weighing from 2333 to 2504 g, were chosen at random for the test. The animals' eyes were examined within 24 hours prior to test material administration using sodium fluorescein dye procedures. Only those animals with no sign of ocular injury or irritation were used. Test animals were identified by animal number and corresponding ear tag. Reason for Species Selection: The New Zealand White albino rabbit is the animal of choice based upon its large orbit and nonpigmented iris. Preparation and Administration of Test Material: The sample was dosed as received. A bulk density determination was made to determine the weight equivalent of a 0.1 ml dose. Because individual doses should not exceed 0.10 g and the weight equivalent of 0.1 ml was 0.12 g, an individual dose of 0.1 g was weighed out for each animal. Treatment: Each rabbit received 0.1 g of the test material placed on the everted lower lid of one eye, with the contralateral eye serving as the untreated control. The upper and lower lids were gently held together for one second to prevent loss of material and then released. The eyes of the rabbits remained unflushed. 001079 H A Z L E T O N LABORATORIES AMERICA. INC. Chemical & BioMedical Sciences Division 3301 KINSMAN BLVD. P.0. BOX 7545 MADISON, WISCONSIN 53707 PHONE (608) 241-4471 TLX 703956 HAZRAL MDS UD SAMPLE HUMBER: 50503501 SAMPLE: T-3752 PAGE 3 OECD EYE IRRITATION (CONTINUED) Observations: The treated eyes were observed for ocular irritation at 1, 24, 48, and 72 hours after treatment. At the 72-hour reading, sodium fluorescein was used to aid in revealing possible corneal injury. Irritation was graded and scored according to the Draize* technique. Animals were weighed just prior to test material administration. Pathology: At study termination all animals were euthanatized and d iscarded. *Draize, J.H., "Appraisal of the Safety of Chemicals in Foods, Drugs, and Cosmetics - Dermal Toxicity." Association of Food and Drug Officials of the U.S., Topeka, Kansas, pp. 49-51 (1959). G01030 H A Z L E T O N LABORATORIES AMERICA. INC. Chemical & BioMedical Sciences Division 3301 KINSMAN BLVD. P.O. BOX 7545 MADISON, WISCONSIN 53707 PHONE (608) 241-4471 TLX 703956 HAZRAL MDS UD SAMPLE NUMBER: 50503501 SAMPLE: T-3752 PAGE 4 OECD EYE IRRITATION (CONTINUED) SUMMARY Test Animal: Albino rabbits - New Zealand White Source: Hazleton Research Products, Inc., Denver PA Date Animals Received: 05/21/85 Temperature and Humidity of Animal Room: 21 to 23 Degrees C. J 46 to 6496 Relative Humidity Test Material: T-3752 Date Test Started: 05/29/85 Date Test Completed: 06/01/85 PRIMARY EYE IRRITATION SCORES* OBSERUATI ON PERIOD 3 Rabbit Mean 0.1 g (Unwashed) 1 Hour: 24 Hours : 48 Hours : 72 Hours : 10.7 0.0 0.0 0.0 * The Primary Eye Irritation Score is the total eye irritation score for all the animals divided by the number of animals (3) at each observation period. Comments: No pain response (vocalization) was elicited from any animal following instillation of the test material. No corneal irritation was observed during the study. 001081 H A Z L E T O N LABORATORIES AMEBICA. INC. Chemical & BioMedical Sciences Division 3301 KINSMAN BLVD. P.O.BOX 7545 MADISON, WISCONSIN 53707 PHONE (608) 241-4471 TLX 703956 HAZRAL MDS UD SAMPLE HUMBER: 50503501 SAMPLE: T-3752 PAGE 5 OECD EYE IRRITATION Animal Number F08711 FO8705 F08721 Observt ion Period 1 Hour 24 Hours 48 Hours 72 Hours 1 Hour 24 Hours 48 Hours 72 Hours 1 Hour 24 Hours 48 Hours 72 Hours (CONTINUED) Table 1 Individual Eye Irritation Scores Cornea Score A B AXBX5 Iris Score A AX5 Con junct ivae Score A B C (A +B +0 2 00 0 1 5 12 0 6 00 0 0 0 000 0 00 0 0 0 000 0 00 0 0 0 000 0 00 0 0 0 110 4 00 0 0 0 000 0 00 0 0 0 000 0 00 0 0 0 000 0 00 00 00 00 0. 0 0 0 1 0 0 0 5 1 2 3 12 0 000 0 0 000 0 0 000 0 Table 2 Sodium Fluorescein Examination Animal Number F08711 F08705 F08721 Observt ion Period P r e - initit ion 72 Ho NEG NEG NEG NEG NEG NEG NEG = No stain retention POS ** Positive stain retention (area of cornea involved). References : 1. Organisation for Economic Cooprt ion and Development's Guidelines for Testing Chemicals, Section 405, Acute Eye Irritt ion/Corrosion, adopted May 12, 1981. 2. Draize J.H., "Appraisal of the Safety of Chemicals in Foods, Drugs, and Cosmetics - Dermal Toxicity", Association of Food and Drug Officials of the United States, Topeka, Kansas, pp. 49-51 (1959). C010S2 PAGE 6 QUALITY ASSURANCE STATEMENT Primary Eye Irritation Study in Rabbits Study No. 50503501 The report as herein attached for the above-mentioned study has been reviewed by the assigned Quality Assurance Unit of Hazleton Laboratories America Inc. in accordance with the Good Laboratory Practice Regulations as set forth in 21 CFR 58.35 (b) (6) (7). It has been found to accurately identify and/or describe the authorized methods and standard operating procedures followed in the conduct of the study and that the reported data accurately reflect the raw data of the laboratory study. Furthermore, the Quality Assurance Unit has conducted the following inspections of the testingfacilities utilized in the conduct of this study and has submitted written reports of said inspections to the study director and/or management. Date of Inspection 5/21-23/85 6/10/85 Type of InspectionProcess Audit Report Review Date Issued to Management 5/23/85 6/10/85 Diana E. Skalitzky/ +* Inspector, Quality Assurance Unit Date 001083 BESTCOPY AVAILABLE PROTOCOL - ATTACHMENT 1 (1) Carrtt (A) QnacH* - degree of denalty (area seat dan takaa for reading) No opacity ... ... -- .... ..-- ..I...... 0 Aaattartd or dlfruat area, dotalla of irla clearly ialble 1 Coolly dlaoernlble translueaat areaa, detail* of irla aUghtly obaoured -- -- -- -- -- ---- -- -- -- -- .... -- -- 2 Opolaaamt area*, no detail* of Irla vlalblo, alsa of pupil barely dlaeernibla ......... - ....... ...... 3 Opaque, Irla lorlalble ..-- -- -- -- -- .-- ...... .. 1 < acu ajCsacau toTBlrrt Oaa quartar (or leaa), but net taro ... ......... . Qroatar tbaa oaa quarter, Out loaa than half n..-- ...... .... Creator than taalf. Out Leaa tOaa t&roe quartara '< ... .. Creator tbaa tOroa quartara, up to uoelr-area -- -- 1 2 3 * AsIxS Total Maxima >0 (2) IdA (A) Talma . Monial ..-- -- -- folia aOoao noraal, ooegeatloo, aeelUag, olreneoroeal Injection (aoy or all of toeae or ooeblnotloo of aay toereef) Irla atUl reacting 0 Uaftt (alugglan reaotloo la pooltlvo) 0 roaatloa to UaOt, beeorrbaga, groaa Oaatruotloo (aay or all of UMao) ...... . .... .............. -- 0 1 2 AXS Total Maalsiat a 10 (3) CoaiuacUxu (A) SKtBtSA (rtfora to palpebral eoejunotlraa only) Toaaala aoreal ........... ...... ............i... Toaaola definitely iajoesed aber* aoraal ... !' Mara Olffuao, deeper orlaaoe red, ladleldual aeaaela aot oaally dlaoernibla .... Olffuao beefy red ....... . ......... -- -- 0 1 2 3 I* availing . .. . . . . . . . . . . ...... i..... . . n.. . -- lay availing abort noreal (laoludaa aletltatls atabraae) ...-- Obeloue availing ulta partial teeraloa of Uda . ......... -- Suelliag vlto Uda about naif eloaad .... ................ - Ivelling alto Uda about half elated to eaeplottly oloaed -- 0 1 2 3 t <C) SttSbMSUt le dlaettarga -- lay aavuan dlfTi A free aoraal (data aot iaolude anil aeouata la laetr eaatftua of noraal anleala) .. . ".. . MaoOarge ulta aoiataolag of tbo Uda aad halre juat adjaeont Uda BlaeOarge wltb aeiataalag of toe Uda aad Oalra, and aeoaldarabla tOa aye ................. leere (A I * C> a 2 Total Masleua 20 0 1 2 3 TOe total more for tbo tya la toe *ue of all aeorte oOtaiaad far to* ooraaa, irla. aad eonjuaeilra*. .601084 Primary Eye Irritation Test Initial Sodium Fluorescein Exam and Animal Body Weights Test Compound T - 3 - t S z t pH Result ___ J3L 0080 <8) O Q a f Dose (ml) /ft Date Animals Received 5~-P/ - Source: r a z L ik t o v 7 f? (r. .& yrch. P r o d u c i - ** RT No. S c 5~o 3>c>! Room No. Dosed By K Reviewed By Date St fil/ tS Date ^ J s y 8 i Animal No. F-S7II Initial Sex SF* 0* Vocaliza tion Following Dosing a! Animal Body Weights (g) Initiation V Day 7 Day 14 5333 S7ai ? a!T IO S 0 * A J& /J t(o a! 2 V 5 7- . as V e Day 21 \ \ V\ \ N TECHNICIAN RECORDED BY DATE /fUS" 3 * SCALE USED Swf NEG " Negative POS Positive NA Not Applicable Y - Yes N - No ck . K yok J & 3 _______________ , rnfc. 0 / ti-j - - - /rrt T3 7- ob$WA.fov\ n '. t is ' r ttr , a t f-sil-?i 001085 Primary Ep Irritation Teat Observation Teat Compound ~T*"* 3 Teat Eve RT No. &OS&3S} .. Croun ___ A / * Sicondi following inacillatioa of toot material the teat eye was vaahed with a/ A ml o A / A for A / f t aeeonds ^Unwoshod Animal No./ iar Tea No. ocation of Corneal Lesions rO " OBSERVATION PERIOD:______ ! h o o \r Tail < ----- ---- > Head r t 3Cular Structure Zornea " Opacity 0 ff n 0o () Q N Iris Area 0 1 xniT 0 0 o () Conjunctivae * - Redness Chemosis I ... 9, ) 1 t \ Discharge O 0 3 _ Sodium Fluorescein Exam m a/A N n Technician ( A ___ C K .. C K tK . OL CK \ Late IA 4 & J2*_ _ % 9 A Purulent Oiaeharge B - Clear Diaeharge C Petite Hemorrhage D Blanching INJ - Injected NEC - Negative POS Positive E Corneal Epithelial Damage Peeling P " Corneal Epithelial Damage, Piling C Corneal Epithelial Damage, Pitting H " Pannua I - Corneal Neovaacularization NA - Not Applicable Reviewed By: Docot O - 3 - f Eye Irritation Score: (1311*1 001086 Primary Eye irritation Test Observations Test Compound - r - i i s s l Toot ty. 1+ Group A / n h o . SoS>3S>l */ft ,Hashed . f t Seconds following instillation of test material the test eye was washed with a/ A ml of A / A for A / A seconds l/ ^ Unwashed OBSERVATION PERIOD: 2V hou/6 A Purulent Discharge B Clear Discharge C Petite Hemorrhage D * Blanching INJ - Injected NEC - Negative POS Positive Reviewed By: E Corneal Epithelial Damage Peeling P Corneal Epithelial Damage Piling C Corneal Epithelial Damage Pitting H'- Pannua I Corneal Neovascularixation NA - Not Applicable Date: Eye Irritation Score: ^ U311AJ 001087 Primary Eye Irritation Test Observations Test Compound - r - n s a . Test Eye Q iO jh J r _ RT No. v5~ Group _ MfL_ j / . l . **<* M E Seconds following instillation of test material the test eye was washed with hJft ml of A / f t for A / A seconds washed Animal No./ -- iar Tag No. r0 ~ .ocation of Zornes1 Lesions oTail <-- --- ---- > Head OBSERVATION PERIOD! Z ( ) ( ) < \) ( ) () Dcular Structure Zornes - Opacity Area Iris 0 o_ D_ 0 Q 0 0 N Conjunctivae - Redness Chemosis Diseharae 0 D 00 Sodium Fluorescein Exam A j f i VfL Technician rx__ nc. Date C K CK _ ,5 : . 3 L ? - 3 ) A Purulent Discharge B Clear Discharge C Petite Hemorrhage D Blanching INJ Injected NEC Negative POS Positive 0 0 A fft a cfc L \ \ \ \ \ E Comesi Epithelial Damage Feeling P Comesi Epithelial Damage Piling G Comesi Epithelial Damage, Pitting H Pannus X Comesi Neovascularisation NA Hot Applicable Reviewed By: Date: Eye Irritation Score: m ) T T a ) ^01088 Primary Eya Irritation Test Observations Test Compound - T - 3 ? s a RI No. S S R S ' f Test Eya Group IL A /* ' Washed . d & -- ,Seconds following instillation of test material, the test eya was washed with a/ A ml of A / A for A /A seconds Unwashed OBSERVATION PERIOD:__________'?3- h o ly 's Animal No./ iar Tat No. rD * .ocation of Corneal Lesions Qrail --------- -- > Head W( 7 Q CL Ccular Structure Cornea - Opacity Area f 0 o Iris Conjunctivae - Redness Chemosis . Discharge 0 a n Sodium Fluorescein Exam o e & Technician Recorded By Pc.. ____ -- <*/( A " Purulent Discharge B - Clear Discharge C - Petite Hemorrhage D Blanching INJ Injected NEC - Negative POS Positive D 0 0 0 o n. /O ea ... V, 0. Q n n 0 Q BSHB N \ \ -\ A v- \\ \ \ E * Corneal Epithelial Damage, Peeling F Corneal Epithelial Damage, Piling C - Corneal Epithelial Damage, Pitting H - Pannus I Corneal Neovascularisation NA Not Applicable Reviewed By Date: M l L .Eye Irritation Score: v u 3u a ) 001089 H A Z L E T O N LABORATORIES AMERICA, INC. 3301 KINSMAN BLVD. P.O. BOX 7545 MADISON, Wl 53707 (608) 2414471 TLX 703956 HAZRAL MDS UD PROTOCOL TP2072 Primary Eye Irritation Study in Rabbits (OECD Guidelines) Study No. 50503501 for 3M St. Paul, Minnesota by Hazleton Laboratories America, Inc. Life Sciences Division 3301 Kinsman Boulevard Madison, Wisconsin 53704 May 21, 1985 * 1985, Hazleton Laboratories America, Inc. Chemical & BioMedical Sciences Division 001090 1 PROTOCOL TP2072 Primary Eye Irritation Study in Rabbits (OECD Guidelines) Study N o . Study Location Test Material 50503501 Hazleton Laboratories America, Inc Life Sciences Division 3301 Kinsman Boulevard Madison, Wisconsin 53704 T-3752 Sponsor's Representative Study Director Janine Gleason Steven M. Glaza Proposed Timetable Starting Date Completion Date Final Report Date Week of May 27, 1985 Week of June 3, 1985 Week of June 24, 1985 001091 OBJECTIVE The objective of this study is to determine the Level of irritation produced following a single exposure of a test material to one eye of albino rabbits. All aspects of this study will conform to the Organisation for Economic Cooperation and Development's Guidelines for Testing Chemicals, Section 405, Acute Eye Irritation/Corrosion, Adopted May 12, 1981* and the 2 Principles of Good Laboratory Practice. All procedures will be done according to Hazleton Laboratories America, Inc. (HLA) Standard Operating Procedures (SOPs) referenced in this protocol. Identification Test Material: Physical Description: Purity and Stability: Storage Conditions: Test Material Retention: Safety Precautions: TEST MATERIAL T-3752. Brown granular solid. Sponsor has purity and stability determinations on file. Store at room temperature. Any unused test material will be returned to the Sponsor 30 days after issuance of final report. Laboratory personnel will take the normal necessary precautions in handling a substance of unknown toxicity. Laboratory clothing, latex gloves, safety glasses, and a particle mask approved for toxic dusts must be worn. 001092 2 TEST SYSTEM Test Animal Young adult albino rabbits of either sex of the New Zealand White strain, approximately 14 weeks of age, will be obtained from Hazleton Research Products, Inc., Denver, Pennsylvania. An adequate number of extra animals will be purchased so that no animal in obviously poor health is placed on test. The New Zealand White albino rabbit is the animal of choice based upon its large orbit and nonpigmented iris. Acclimation Upon receipt, the animals will be taken to a designated animal room where they will be acclimated for at least 1 week before being placed on test (OP-GENB 36). During acclimation, the animals will be examined for clinical abnormalities indicative of health problems (e.g., diarrhea, ectoparasites, rough hair coat, nasal or ocular discharge, evidence of injury, etc.). Any animals regarded as unsuitable for the study purposes because of poor physical condition will not be released from acclimation and the reason(s) will be documented. Identification Each animal in the study will be assigned a permanent identification number and will be identified with a metal ear tag (OP-GENB 24). All data collected from an animal will be recorded and filed under its identification number. 001093 3 Housing and Maintenance The following environmental conditions will be maintained in the animal room used for this study (OP-TARC 230). o Temperature: 21C +2 o Relative humidity: 50% +20% o Air change: At least 10 changes an hour of filtered 100% outside air o Light cycle: 12 hours light/12 hours dark Temperature and humidity will be monitored throughout the study. Variations from prescribed environmental conditions will be documented. Animal husbandry and housing at HLA comply with standards outlined in the 3 "Guide for the Care and Use of Laboratory Animals." Care will be taken to ensure that the animals are not disturbed for reasons other than data collection and routine maintenance. The animals will be housed individually in screen-bottom stainless steel cages (heavy gauge) held on racks, with absorbent pan liners in the urine- and feces-collecting pans. Pan liners will be changed at least three times each week. Feed and water will be provided ad libitum. The diet will be Purina High Fiber Rabbit Chow. No contaminants are expected to be present in the feed or water which would interfere and affect the results of the study. Study Design Three rabbits will be selected at random based upon health and a body weight of 2.0 to 3.5 kg. PROCEDURES Preparation and Administration of Test Material The rabbits' eyes will be examined using fluorescein dye procedures within 24 hours prior to test material administration. Only animals with no sign of 001094 4 corneal injury or eye abnormalities will be utilized. One eye of each animal will be treated with the test material and the other eye will serve as the untreated control. Each rabbit will receive 0.1 g (or the weight equivalent of 0.1 mL) of solid test material. If necessary, the solid test materials will be finely ground into a dust or powder. The test material will be placed into the everted lower lid of the rabbit's eye. The upper and lower lids are then to be gently held together for 1 second before releasing to prevent loss of material. The eyes of the rabbits will remain unflushed for 24 hours following instillation of the test material. After 24 hours, a washout may be used if considered appropriate. Reason for Route of Administration 4 Historically, the route of choice based on the method of Draize. Ob servations The treated eyes of all animals will be examined for ocular irritation at 1, 24, 48, and 72 hours after treatment. If no irritation or injury is present at 72 hours, the study will be terminated. If irritation is present at 72 hours, additional observations will'be made at 96 hours and at 7, 14, and 21 days. If at any of these time points there is no irritation, the study will be terminated. If injury is still present at 21 days, the Sponsor will be contacted to determine whether the study should continue or be terminated. After recording the 24-hour observations, sodium fluorescein may be used to aid in revealing possible corneal injury. Irritation will be graded and 4 scored using the Draize technique (Attachment 1). All eye abnormalities will be recorded. 001095 5 All animals that have a damaged eye producing undue stress or discomfort will be sacrificed for humane reasons after consulting with the Sponsor. Body weights will be recorded prior to test material administration and at weekly intervals throughout the study. Observations and body weights will be recorded in the study notebook. Pathology All animals, whether dying or sacrificed at study termination, will be discarded. Re por t The final report will present a description of the test material, a description of the test system, dates of study initiation and termination, a summary table showing the irritation data at each observation period, and any special observations that were recorded. Maintenance of Raw Data and Records Original data or copies thereof will be available at HLA to facilitate auditing the study during its progress and prior to acceptance of the final report. When the final report is completed, all original paper data, as well as the final report, will be retained in the archives of HLA, Madison, Wisconsin (OP-GEN 44). 001096 6 REFERENCES 1. "Acute Eye Irritation/Corrosion," OECD Guidelines for Testing Chemicals, Section 405 (May 12, 1981). 2. Organisation for Economic Cooperation and Development's Principles of Good Laboratory Practice, Annex 2, 1981. 3. DHEW Publications No. (NIH) 78-23 (1978). 4. Draize, J. R., Appraisal of the Safety of Chemicals in Foods, Drugs, and Cosmetics - Dermal Toxicity, Association of Food and Drug Officials of the U.S., Topeka, Kansas, pp. 49-51 (1959). 001097 7 PROTOCOL APPROVAL J a m i\e l Gleason Sponsor's Representative 3M Steven M. Glaza Study Director Group Leader, Acute Toxicology Hazleton Laboratories America, Inc . (1109S/tji) sfw lts" Date s-'za.-'& s r 001098 PROTOCOL - ATTACHMENT 1 (1) Cam^ (A) Opacity - degree of density (area sost dense taken for readins) Ho opacity Scattered or diffuse ares, details of iris dearly visible -- -- -- Easily discernible translucent areas, details of iris slightly obscured -- -- .. Opalescent areas, no details of iris visible, size of pupil barely discernible -- -- -- -- -- ----------------- Opaque, iris invisible --------- .. -- 0 1 2 3 t (B) ires of comes involved One quarter (or less), but not zero -- ... m-- -- Greater than one quarter, but less than half Greater than half, but less than three quarters ....... -- ... Greater than three quarters, up to whole area 1 2 3 < 1x Bz 5 Total Maxlaua * 80 (2 ) I d a (A) Talues Moraal ... Folds above noraal, congestion, swelling, circuacoraeal injection (any or all of these or eoablnatlon of any thereof) iris still - reacting tc light (sluggish reaction is positive) Ho reaction to light, beaorrhage, gross destruction (any or all of these) -- -- -- -- ---- ...... '.... . 0 1 2 lt$ Total Maxlaua 10 (3) Can.lunctivee (A) Hedneaa (refers to palpebral conjunct!vae only) Teasels noraal .-- . .. -- .- Tesaela definitely injeoted above noraal -- .. More diffuse, deeper orinsoa red, individual vessda not easily disoemible .. Biffuae beefy red .-.... ..... 0 .j *2 3 (B) Ho swelling -- ------- -- -- -- -- Any swelling above noraal (includes nictitating oeabrsne) ---... Obvious swelling with partial eversion of lids -- -- ------------ -- Swelling with lids about half closed --- --- ..- -- -- - -- Swelling with lids about half dosed to eonpletely dosed -- -- -- -- (C) BlachBCM Ho discharge ....< Any aaount different free noraal (does not Indude snail amounts observed in inner canthus of norad animals) -- -- -- -- -- -- -- -- Discharge with aoistening of the lids and hairs just adjacent to lids ..........-- -- -- ...--- - -- ... .2 Discharge with aoistening of the lids and hairs, and considerable . area around the eye -- -- -- -- -- -- -r-- -- -- -- -- -- -- -- 0 1 2 3 4 0 1 3 Score (A * B * C) x 2 Total Maxlaua 20 Tha total score for ths eye is the sue of all scores obtained for the cornea, irla, and conjunctivas. 001093