Document 0egDe175pkJOXzLwrdKZb0nM

X tH s.} COV/VitfCE> THE DEVELOPMENT SERVICES COMPANY 0 0 * U :1 2 Summary Report 104-Week Dietary Carcinogenicity Study with Narrow Range (98.1%) N-Ethyl Perfluorooctanesulfonamido Ethanol in Rats PREPARED FOR: 3M COVANCE STUDY NUMBERS: 6329-212 and-228 003113 SUMMARY REPORT - WEEK 53 104-Week Dietary Carcinogenicity Study with Narrow Range (98.1%) N-Ethyl Perfluorooctanesulfonamido Ethanol in Rats Timelines Covance 6329-212: In-life start date: January 26, 1998 Week 53 completed: January 31,1999 Covance 6329-228: In-life start date: April 6, 1998 Week 53 completed: April 11, 1999 Introduction The purpose of these studies was to assess the carcinogenicity of the test material, T-6316 (Narrow Range N-Ethyl Perfluooctanesulfonamido Ethanol, NEtFOSE), when administered in the diet to rats for at least 104 weeks. This summary report includes information through Week 53 and contains current findings; it is not, however, the final report for the study. Covance Laboratories Inc., reserves the right of review of these data before issuing the final report. Methods Male and female Crl:CD(SD)IGS BR rats were assigned to groups according to the following design. Group No. of Animals Male Female Dietary Levels * (ppm NEtFOSE) Covance 6329-212 1 (Control)b 2 (Low) 3 (Mid) 4 (Mid-High) 5 (High) 6 (Mid-High Recovery) 7 (High Recovery) 70 70 60 60 60 60 70 70 70 70 40 40 40 40 0 3 30 100 300 100 300 Covance 6329-228 8 (Control) b 9 (Treated) 70 70 70 70 0 1 a T-6316 is 98.1% n-ethyl perfluorooctanesulfonamido ethanol (NEtFOSE); dose levels are expressed as ppm of NEtFOSE. b The control animals received the basal diet only. 1 o lt4 Covance 6329-212/228 _______________________ ,_____________________________________________________ 3MT-6316.1 Food was provided ad libitum, except when animals were fasted. Water was provided ad libitum. The animals were observed twice daily (a.m. and p.m.) for mortality and moribundity; findings were recorded as they were observed. At least once prior to treatment and weekly thereafter, each animal was removed from its cage and examined; abnormal findings or an indication of normal was recorded. Body weight data were recorded weekly for each animal for Weeks 1 through 17 and once every 4 weeks thereafter. Food consumption data were collected for each animal for Weeks 1 through 16 and once every 4 weeks thereafter. Body weights were also recorded for all animals in Groups 5 and 7 before sacrifice during Week 8. Clinical Pathology. Blood and urine samples were collected for hematology, clinical chemistry, urinalysis, and urine chemistry from five animals/sex/group in Groups 1 and 5 during Week 8. Blood and urine samples were collected for hematology, clinical chemistry, urinalysis, urine chemistry, and serum sampling from 10 animals/sex/group in Groups 1 through 4 during Weeks 14, 27, and 53. Blood and urine samples were collected for hematology, clinical chemistry, urinalysis, and urine chemistry from 10 animals/sex/group from animals in Groups 8 and 9 during Weeks 14, 27, and 53. Animals were fasted overnight, and urine was collected chilled overnight (approximately 16 hours) before blood sampling. Blood was collected from a jugular vein. The anticoagulant was potassium EDTA for hematology tests. The samples for clinical chemistry and serum samples were collected without anticoagulant. The serum samples (that serum not used for clinical chemistry) were stored in a freezer set to maintain -60 to -80C, until shipped to the Sponsor for possible future analysis. When possible, a blood film was made for possible future examination from animals sacrificed at unscheduled intervals. A blood film was also made and held for possible future examination for animals at the scheduled sacrifice during Week 53. Serum Analyses. Blood samples were collected for serum analyses from five animals/sex/group in Groups 1 through 5, 8, and 9 during Week 4; five animals/sex/group in Groups 1 and 5 during Week 8; five animals/sex/group in Groups 1 through 4, 8, and 9 during Week 14; and five animals/sex/group from Groups 1, 4, 8, and 9 during Week 53. Animals were fasted overnight. Blood (approximately 2 mL during Weeks 4, 8, and 14, and approximately 3 mL during Week 53) was collected from a jugular vein. Samples were collected without anticoagulant. Serum was harvested, stored in a freezer, set to maintain -60 to -80C, until sent to the Sponsor for possible future analyses. 2 O O u llS Covance 6329-212/228 ________ 3MT-6316.1 Termination. During Week 4, five animals/sex/group in Groups 1 through 5, 8, and 9 were fasted overnight, bled for serum samples, anesthetized with sodium pentobarbital (Groups 1 through 5) or carbon dioxide (Groups 8 and 9), weighed, and exsanguinated. The abdominal cavity of each animal was opened, the liver was removed and weighed, and liver samples were collected. Animals were discarded after liver collection. During Week 8, five animals/sex/group in Groups 1 and 5 were fasted overnight, bled for clinical pathology and serum analyses, anesthetized with sodium pentobarbital, weighed, exsanguinated, and necropsied. At necropsy, macroscopic observations were recorded, selected organs were weighed, and selected tissues were collected and preserved. Additional liver samples were also collected. Adrenals, brain, eyes, kidneys, liver, mesenteric lymph node, pancreas, spleen, testes, and ovaries were processed and examined microscopically. The remaining animals in Groups 5 and 7 were euthanatized with carbon dioxide and discarded without necropsy. During Week 14, five animals/sex/group in Groups 1 through 4 were fasted overnight, bled for clinical pathology and serum analyses, anesthetized with carbon dioxide, weighed, exsanguinated, and necropsied. At necropsy, macroscopic observations were recorded, selected organs were weighed, and selected tissues were collected and preserved. Additional liver samples were also collected. Adrenals, brain, eyes, kidneys, liver, mesenteric lymph node, pancreas, spleen, testes, and ovaries were processed and examined microscopically. Also during Week 14, five animals/sex/group in Groups 8 and 9 were fasted overnight, bled for clinical pathology and serum samples, anesthetized with carbon dioxide, weighed, and exsanguinated. The abdominal cavity of each animal was opened, the liver was removed and weighed, and liver samples were collected. Animals were discarded after liver collection. During Week 53, five animals/sex from Group 1 and 10 animals/sex from Groups 4, 8, and 9 were fasted overnight, bled for clinical pathology and serum analyses (as appropriate), anesthetized with carbon dioxide, weighed, exsanguinated, and necropsied. At necropsy, macroscopic observations were recorded, selected organs were weighed, and selected tissues were collected and preserved. Additional liver samples were also collected. Tissues from each animal in Groups 1 and 4 were processed and examined microscopically. Animals that died on test or were sacrificed at an unscheduled interval were also necropsied, but organ weights were not recorded and liver samples were not collected. 3 G0411G Covance 6329-212/228 ________________________ ,____________________________________________________ 3M T-6316.1 Proliferation Cell Nuclear Antigen (PCNA) Evaluation. At the Week 4 sacrifice for animals in Groups 1 through 5 (five animals/sex/group), representative samples of the left lateral, right median, and right lateral lobes of the liver and any macroscopic lesions of the liver were collected and preserved in zinc formalin. At the Week 8 sacrifice for animals in Groups 1 and 5 (five animals/sex/group), representative samples of the left lateral lobe of the liver and any macroscopic lesions of the liver were collected and preserved in zinc formalin. At the Week 14 sacrifice for animals in Groups 1 through 4, 8, and 9 (five animals/sex/group), representative samples of the left lateral lobe of the liver and any macroscopic lesions of the liver were collected and preserved in zinc formalin. Liver samples were embedded, and blocks were sent to Pathology Associates International (PAI); PAI will evaluate the samples (left lateral lobe only) for PCNA. In addition, liver sections will be stained with hematoxylin and eosin and examined microscopically. Mitochondrial Analyses. At the Week 4 sacrifice for animals in Groups 1 through 5 (five animals/sex/group), a sample (approximately 1.5 g) of the liver was collected from selected animals. Samples were placed on wet ice and transferred to the Sponsor for mitochondrial analysis. Palmitoyl-CoA (PCoA) Oxidase Analyses. At the Week 4 sacrifice for animals in Groups 1 through 5, 8, and 9 (five animals/sex/group), a sample (approximately 500 mg) of the right lateral lobe of the liver was collected and flash-frozen in liquid nitrogen. At the Week 8 sacrifice for animals in Groups 1 and 5 (five animals/sex/group), a sample (approximately 500 mg) of the right lateral lobe of the liver was collected and flash-frozen in liquid nitrogen. At the Week 14 sacrifice for animals in Groups 1 through 4, 8, and 9 (five animals/sex/group), a sample (approximately 500 mg) of the right lateral lobe of the liver was collected and flash-frozen in liquid nitrogen. The samples were stored in a freezer, set to maintain -60 to -80C, until analyzed by Covance for PCoA oxidase activity. Additional Liver Sample Collection. At the Week 4 sacrifice for animals in Groups 1 through 5, 8, and 9 (five animals/sex/group), a portion of the liver was stored in a freezer, set to maintain -60 to -80C, until sent to the Sponsor for possible future analysis. At the Week 8 sacrifice for animals in Groups 1 and 5 (five animals/sex/group), a portion of the liver was stored in a freezer, set to maintain -60 to -80C, until sent to the Sponsor for possible future analysis. At the Week 14 sacrifice for animals in Groups 1 through 4, 8, and 9 (five animals/sex/group), a portion of the liver was stored in a freezer, set to maintain -60 to -80C, until sent to the Sponsor for possible future analysis. 4 ooai.17 Covance 6329-212/228 ________ 3M T-6316.1 Results Survival. Adjusted survival data are summarized in Tables 1 and 2. Because of toxic effects seen for the body weights and food consumption at 300 ppm, the animals in Groups 5 and 7 were sacrificed early (during Week 8). For the remaining groups, survival after 53 weeks of treatment was 98.0%, 96.0%, 98.0%, 96.0%, 90.0%, 96.0%, and 96.0% for males in Groups 1, 2, 3, 4, 6, 8, and 9, respectively, and 98.0%, 100%, 98.0%, 96.0%, 100%, 92.0%, and 96.0% for females in Groups 1, 2, 3,4, 6, 8, and 9, respectively. Clinical Observations. Clinical observation data are summarized in Tables 3 and 4. There were no apparent test material-related observations noted through Week 53. Body Weights. Body weight and body weight change data are summarized in Tables 5 through 8. Animals given 100 or 300 ppm had significantly lower body weights compared to those of animals given the control material starting after the first week of treatment. Animals given 30 ppm had lower body weights compared to those of animals given the control material starting after the first week of treatment; however, they were not generally significant until approximately Week 8. There were no toxicologically meaningful significant body weight differences for animals given 1 or 3 ppm. Animals given 300 ppm had significantly lower body weight changes compared to those of animals given the control material starting with the first week of treatment. Animals given 100 ppm had numerous significantly lower body weight changes compared to those of animals given the control material, and the overall body weight change (Week 1-53) was significantly lower than those of animals given the control material. Animals given 30 ppm had sporadic significantly lower body weight changes compared with those of animals given the control material, and the overall body weight change (Week 1-53) was significantly lower than those of animals given the control material. There were no toxicologically meaningful significant body weight change differences for animals given 1 or 3 ppm, and the overall body weight change (Week 1-53) was not significantly different than those of animals given the control material. 5 coans Covance 6329-212/228 ______________________________________________________________________________3M T-6316.1 Food Consumption. Food consumption data are summarized in Tables 9 and 10. Animals given 100 or 300 ppm had significantly lower food consumption compared to those of animals given the control material starting with the first week of treatment. Males given 30 ppm had numerous significantly lower food consumption intervals compared to those of animals given the control material starting with Week 3. There were no toxicologically meaningful significant differences for females given 30 ppm or for animals given 1 or 3 ppm. Test Material Consumption. Test material consumption data are summarized in Tables 11 and 12. Animals were fed diets containing 0,1, 3, 30,100, and 300 ppm. The amount of test material consumed by animals on a mg/kg of body weight/day basis was as follows. Target Dose Levels (ppm) 1 3 30 100 300 100 300 Achieved Dose Levels - Range (mg/kg body weight/day) Males Females 0.036-0.103 0.049-0.108 0.108-0.259 0.147-0.307 1.126-2.618 1.454-3.070 3.847-8.724 5.686-10.166 16.058-25.408 19.568-28.712 3.841-8.873 5.355-10.135 15.803-25.193 20.004-29.270 Clinical and Anatomic Pathology. Hematology, clinical chemistry, urinalysis, and urine chemistry data are summarized in Tables 13 through 42. Terminal body weights, absolute organ weights, organ-to-body weight percentages, and organ-to-brain weight ratios are summarized in Tables 43 through 49. Incidences of macroscopic and microscopic observations are summarized in Tables 50 through 59. Incidence of severity of selected microscopic observations is summarized in Tables 60 through 62. The Pathology Report contains a discussion of the data. 6 00,3119 Covance 6329-212/228 ________________________t____________________________________________________ 3M T-6316.1 Dose Analyses. Results of the homogeneity, stability, and dose preparation analyses are in Tables 63 through 68. Prestudy, several homogeneity analyses were conducted on each of several mixes in an attempt to resolve issues related to the inherent variability and lack of sufficient sensitivity at low levels in the analytical method for measuring NEtFOSE. Mean values of the homogeneity analyses for the mix done on January 15,1998, ranged from 89.7-104%, 92.0-98.7%, 86.8-98.4%, and 94.0-96.7% of the theoretical concentrations for the diets containing 3, 30, 100, and 300 ppm NEtFOSE, respectively. Mean values of the homogeneity analyses for the mix done on March 30,1998, ranged 91.3-106% of the theoretical concentrations for the diet containing 1 ppm NEtFOSE. These results indicate that the mixing procedure produced a homogeneous distribution of the test material in the dose preparations; although variability generally appeared slightly greater at the lower dietary concentrations. Prestudy, several stability analyses were conducted on each of several mixes in an attempt to resolve issues related to the inherent variability and lack of sufficient sensitivity at low levels in the analytical method for measuring NEtFOSE. Results of stability analyses for the mix conducted on October 15,1997, of samples stored for 19 days at room temperature conditions indicated that the mean concentrations were 89.8 % and 87.5% of the initial concentrations of 3 ppm and 300 ppm respectively. Results of stability analyses for the mix conducted on October 15, 1997, of samples stored for 34 days at room temperature conditions indicated that the mean concentrations were 90.4 % and 89.6% of the initial concentrations of 3 ppm and 300 ppm respectively. Results of stability analyses for the mix conducted on October 15, 1997, of samples stored for 8 weeks under frozen conditions indicated that the mean concentrations were 89.2% and 95.3% of the initial concentrations of 3 ppm and 300 ppm respectively. Results of stability analyses for the mix conducted on June 5,1998, of samples stored 32 days at room temperature conditions indicated that the mean concentration was 77.9% of the initial concentration of 1 ppm. Results of stability analyses for the mix conducted on June 5,1998, of samples stored at room temperature for 46 days conditions for indicated that the mean concentration was 93.4% of the initial concentration of 1 ppm. 7 GOoJ.20 Covance 6329-212/228 3M T-6316.1 The mean concentrations of the dose preparation analyses for all levels ranged from 61.8%-134% of the theoretical concentrations (including the reassay and retention sample analyses, but excluding data that required reanalysis). Inherent variability and lack of sufficient sensitivity at low levels in the analytical method for measuring NEtFOSE resulted in homogeneity, stability, and routine analyses data that were in many cases outside of the standard limits of 15%. These results will be reevaluated in conjunction with the analytical results from the blood and liver level determinations when they are provided by the Sponsor. 8 0031,31 PATHOLOGY REPORT Covance 6329-212/228 ________ 3M T-6316.1 SUMMARY The purpose of these studies was to assess the carcinogenicity of the test material, N-Ethyl Perfluorooctanesulfonamido Ethanol (NEtFOSE) when administered in the diet to rats for at least 104 weeks. The test material was administered at dose levels of 1, 3, 30, 100, and 300 ppm. Animals given 300 ppm were sacrificed during Week 8. This interim report discusses findings through the first 53 weeks of the study. Dietary administration of NEtFOSE was associated with mildly decreased red blood cell count, hemoglobin, and hematocrit for females given 100 ppm; mildly lower glucose and mildly higher urea nitrogen for males and females given 100 ppm (predominantly at Weeks 14 and 27); moderately higher albumin and moderately lower globulin for males given 100 ppm (predominantly at Weeks 27 and 53); and moderately to markedly lower cholesterol for males given 30 or 100 ppm and females given 100 ppm. Administration of NEtFOSE at 1 or 3 ppm for approximately 52 weeks had no effects on clinical pathology test results. At a dose level of 300 ppm for approximately 8 weeks, administration of NEtFOSE was associated with many effects on clinical pathology results including lower red blood cell count, hemoglobin, hematocrit, glucose, globulin, and cholesterol and higher urea nitrogen, albumin, total bilirubin (males only), aspartate aminotransferase (males only), alanine aminotransferase, and hepatic palmitoyl-CoA oxidase (also observed at Week 4). Most of the effects were moderate to marked in magnitude. One male given 3 ppm, three males given 100 ppm, and four males given 300 ppm died on test or were sacrificed moribund. The male given 3 ppm was sacrificed due to a fracture of the hard palate. The cause of death was undetermined for one male given 100 ppm and one male given 300 ppm. All of the other animals that died or were sacrificed at unscheduled intervals had hepatocellular hypertrophy or hepatocellular necrosis due to treatment with the test material, or both. Hepatocellular necrosis was the cause of death for two males given 100 ppm and three males given 300 ppm. 003X22 9 Covance 6329-212/228 ________________________ _____________________________________________________ 3M T-6316.1 At the Week 4 sacrifice, terminal body weights were lower for males given 300 ppm, and absolute and relative liver weights were higher for males and females given 100 or 300 ppm. There were no gross findings at the Week 4 sacrifice, and tissues were not examined histologically. At the Week 8 sacrifice, five animals/sex given 0 or 300 ppm were necropsied. Terminal body weights were lower and absolute and relative liver weights were significantly higher for males and females given 300 ppm. The only test material-related findings at necropsy were large livers for five males and two females given 300 ppm and mottling of the liver of one female given 300 ppm. Microscopically, test material-related findings were limited to the liver and consisted of hepatocellular hypertrophy, hepatocellular necrosis, and hemorrhage. At the Week 14 sacrifice, terminal body weights were lower and absolute and relative liver weights were significantly higher for males and females given 100 ppm. There were no test material-related gross findings noted at necropsy. The only test material-related microscopic finding was hepatocellular hypertrophy for males given 30 or 100 ppm and females given 100 ppm. At the Week 53 sacrifice, terminal body weights were lower and absolute and relative liver weights were significantly higher for males and females given 100 ppm. There were no test material-related gross findings noted at necropsy. Test material-related findings were limited to the liver and consisted of hepatocellular hypertrophy, vacuolation, necrosis, and bile stasis for animals given 100 ppm. 10 003123 METHODS Covance 6329-212/228 _____ 3M T-6316.1 Nine groups of Crl:CD(SD)IGS BR rats were studied using the following study design. Group No. of Animals Male Female Dietary Levelsa (ppm NEtFOSE) Covance 6329-212 1 (Control)b 2 (Low) 3 (Mid) 4 (Mid-High) 5 (High) 6 (Mid-High Recovery) 7 (High Recovery) 70 70 60 60 60 60 70 70 70 70 40 40 40 40 0 3 30 100 300 100 300 Covance 6329-228 8 (Control)b 9 (Treated) 70 70 70 70 0 1 a T-6316 is 98.1% n-ethyl perfluorooctanesulfonamido ethanol (NEtFOSE); dose levels are expressed as ppm of NEtFOSE. b The control animals received the basal diet only. During Week 8, blood and urine were collected for hematology, clinical chemistry, urinalysis, and urine chemistry tests from five animals/sex/group in Groups 1 and 5. During Weeks 14, 27, and 53, blood and urine were collected for these tests from 10 animals/sex/group in Groups 1 through 4, 8, and 9. Blood smears were made for all animals sacrificed at unscheduled intervals or during Week 53 (interim sacrifice). Necropsies were performed on five animals/sex in Groups 1 and 5 sacrificed during Week 8; five animals/sex in Groups 1 through 4 sacrificed during Week 14; and five animals/sex in Group 1 and 10 animals/sex in Groups 4, 8, and 9 sacrificed during Week 53. At necropsy, macroscopic observations were recorded, organ weights were obtained (for sacrifices during Weeks 8, 14, and 53), and tissues were placed in fixative as specified by the protocol. At unscheduled necropsies for animals that died or were sacrificed because of poor health, macroscopic observations were recorded, and tissues were placed in fixative, but organ weights were not obtained. Microscopic examinations were done on selected tissues from animals in Groups 1 through 5 necropsied during Weeks 8 and 14. The tissues were adrenals, brain, eyes, kidneys, liver, mesenteric lymph node, pancreas, spleen, testes, and ovaries. Microscopic examinations were done on all collected tissues from animals in Groups land 4 necropsied at the Week 53 interim sacrifice. 11 003124 Covance 6329-212/228 ________ 3M T-6316.1 In addition, microscopic examinations were done on tissues from animals that died or were sacrificed due to poor health. Five animals/sex in Groups 1 through 5 were sacrificed during Week 4, and five animals/sex in Groups 1 through 4 were sacrificed during Week 14 for hepatocellular proliferation rate measurements and hepatic palmitoyl-CoA oxidase activity. At the Week 4 collection for animals in Groups 1 through 5, samples of liver were collected for mitochondrial analysis. Five animals/sex in Groups 8 and 9 were sacrificed during Week 4 for hepatic palmitoyl-CoA oxidase activity and during Week 14 for hepatocellular proliferation rate measurements and hepatic palmitoyl-CoA oxidase activity. Statistically significant differences cited in the Results and Discussion section are based on comparisons between the control and treated groups. RESULTS AND DISCUSSION Clinical Pathology Week 4. Males and females given 300 ppm had mildly, but statistically, higher hepatic palmitoyl-CoA oxidase activity consistent with peroxisomal proliferation. Week 8. There were many statistically significant or otherwise notable differences for clinical pathology results between animals given the control material and animals given 300 ppm. Those differences considered to be effects of the test material were lower red blood cell count, hemoglobin, hematocrit, glucose, globulin, and cholesterol and higher urea nitrogen, albumin, total bilirubin (males only), aspartate aminotransferase (males only), alanine aminotransferase, and hepatic palmitoyl-CoA oxidase. With the exception of higher alanine aminotransferase for the females, these differences were statistically significant. The effects on the erythrocyte parameters, glucose, and globulin were consistent with poor health and failure to gain weight appropriately. Higher urea nitrogen and albumin were most consistent with mild dehydration and indicated the effects on other parameters (e.g., the erythrocyte parameters) would likely have been greater if the animals were normovolemic. The effects on alanine aminotransferase, aspartate aminotransferase, and total bilirubin were indicative of the hepatocellular degeneration and necrosis observed microscopically. 12 0 0 ^ 1 2 5 Covance 6329-212/228 ________ 3M T-6316.1 Other statistically significant differences were considered to be of uncertain relationship to administration of the test material. Mildly lower platelet count was observed for males but not females. Absolute eosinophil count was statistically lower for both males and females, but the differences were extremely small as normal eosinophil count is very low. Weeks 14,27, and 53. There were several statistically significant or otherwise notable differences for clinical pathology results between control and treated animals, Those differences considered to be effects of the test material were not always statistically significant but were generally consistent over time and exhibited a relationship to dose. Several statistically significant differences that were inconsistent over time or did not affect the high-dose group were considered incidental. Administration of the test material was associated with decreased red blood cell count, hemoglobin, and hematocrit for females given 100 ppm; lower glucose and higher urea nitrogen for males and females given 100 ppm (predominantly at Weeks 14 and 27); higher albumin and lower globulin for males given 100 ppm (predominantly at Weeks 27 and 53); and lower cholesterol for males given 30 or 100 ppm and females given 100 ppm. These effects were consistent with those observed at Week 8 for animals given 300 ppm but were less prominent. The effect of greatest magnitude was that for cholesterol. At the highest dose level, cholesterol was markedly decreased for the males. The erythrocyte effects and the effects on glucose and urea nitrogen were very mild, and the serum protein effects were considered of moderate severity. Administration of the test material at 1 or 3 ppm for approximately 52 weeks had no effect on clinical pathology test results. Anatomic Pathology Unscheduled Deaths. One Group 1 control male (Animal No. C90721) and one Group 1 control female (Animal No. C91179), two Group 2 males given 3 ppm (Animals Nos. C90806 and C90813), one Group 3 male given 30 ppm (Animal No. C90878), one Group 3 female given 30 ppm (Animal No. C91282), two Group 4 males given 100 ppm (Animals Nos. C90945 and C90949), one Group 4 female given 100 ppm (Animal No. C91322), three Group 5 males given 300 ppm (Animal Nos. C90993, C91003, and C91029), four Group 6 males given 100 ppm (Animal Nos. C91045, C91046, C91049, 13 003X 2C Covance 6329-212/228 ______________________________________________________________________________3M T-6316.1 and C91068), one Group 7 male given 300 ppm (Animal No. C91099), two Group 8 control males (Animal Nos. C92198 and C92213), four Group 8 control females (Animal Nos. C92235, C92342, C92345, and C92347), two Group 9 males given 1 ppm (Animal Nos. C92273 and C92293) and two Group 9 females given 1 ppm (Animal Nos. C92403 and C92437) died on test or were sacrificed moribund. Animal No. C90721, a Group 1 control male, died due to a widespread malignant lymphoma. Animal No. C90806, a Group 2 male given 3 ppm, was sacrificed because of a large subcutaneous undifferentiated sarcoma, and Animal No. C90813, a Group 2 male given 3 ppm, was sacrificed because it had a fracture of the hard palate. Animal No. C91003, a Group 5 male given 300 ppm, and Animal No. C91282, a female given 30 ppm, were sacrificed moribund with pituitary adenomas. Animals Nos. C91045, C91046, and C91068, Group 6 males given 100 ppm, and Animal No. C91099, a Group 7 male given 300 ppm, were sacrificed or died due to hepatic necrosis. Animal No. C91049, a Group 6 male given 100 ppm, died with necrosis of the musculature of the anus. Animal No. C91179, a Group 1 control female, was sacrificed because of a large mammary fibroadenoma. The cause of death was undetermined for the other Group 1 to 7 animals. Histopathology was not done on the Group 8 and 9 animals. The macroscopic findings were typical of those observed in rats in long term studies. Week 4 Sacrifice Organ Weights. Terminal body weights were lower for males given 300 ppm. Absolute and relative liver weights were higher for males and females given 100 or 300 ppm. There were no other test material-related changes for absolute organ weights, organ-to-body weight percentages or organ-to-brain weight ratios. Macroscopic Findings. There were no gross findings noted at necropsy. Week 8 Sacrifice Organ Weights. Terminal body weights were lower and absolute and relative liver weights were significantly higher for males and females given 300 ppm. There were no other test material-related changes for absolute organ weights, organ-to-body weight percentages, or organ-to-brain weight ratios. 14 00C127 Covance 6329-212/228 ________ 3M T-6316.1 Macroscopic Findings. The only test material-related findings at necropsy were large livers for five males and two females given 300 ppm and mottling of the liver of one female given 300 ppm. There were no other test material-related gross findings noted at necropsy. Microscopic Findings. Test material-related findings were limited to the liver. The test material-related hepatic findings were hepatocellular hypertrophy, hepatocellular necrosis, and hemorrhage. The incidence and severity of the hepatic lesions are listed in Text Table 1. Text Table 1 Incidence and Severity of Test Material-Related Microscopic Findings Males_____ Females N-Ethyl Perfluorooctanesulfonamido (ppm) Dose Level Number examined f l 300 5 Q 300 51 Liver Hypertrophy Moderate 0 5 04 Moderately-severe 0 0 01 Necrosis Minimal 0 1 00 Moderate 0 2 01 Hemorrhage Minimal 0 3 00 Moderate 0 0 01 Week 14 Sacrifice Organ Weights. Terminal body weights were lower and absolute and relative liver weights were significantly higher for males and females given 100 ppm. There were no other test material-related changes for absolute organ weights, organ-to-body weight percentages, or organ-to-brain weight ratios. Macroscopic Findings. There were no test material-related gross findings noted at necropsy. 15 Covance 6329-212/228 ________ 3M T-6316.1 Microscopic Findings. The only test material-related finding was hepatocellular hypertrophy for males given 30 or 100 ppm and females given 100 ppm. The incidence and severity of the hepatocellular hypertrophy are listed in Text Table 2. Text Table 2 Incidence and Severity of Hepatocellular Hypertrophy Dose Level Number examined Males_____ Females N-Ethyl Perfluorooctanesulfonamido (ppm) f l 3 20 100 Q 2 20 100 55 5 5 555 5 Liver Hypertrophy Minimal Slight Moderate 00 1 0 000 0 00 1 4 000 2 00 1 1000 0 Week 53 Sacrifice Organ Weights. Terminal body weights were lower and absolute and relative liver weights were significantly higher for males and females given 100 ppm. There were no other test material-related changes for absolute organ weights, organ-to-body weight percentages, or organ-to-brain weight ratios. Macroscopic Findings. There were no test material-related gross findings noted at necropsy. Microscopic Findings. Hepatocellular hypertrophy and vacuolation of the liver were observed only for animals given 100 ppm. The incidence and severity of hepatocellular hypertrophy and vacuolation are listed in Text Table 3. Other test material-related hepatic findings were hepatocellular necrosis for one male and two females given 100 ppm and bile stasis for one male and two females given 100 ppm. One female given 100 ppm had a hepatocellular adenoma. Hepatocellular adenomas are occasionally observed as an incidental finding in 1 year old rats. 16 Covance 6329-212/228 ________ 3M T-6316.1 Text Table 3 Incidence and Severity of Hepatocellular Hypertrophy Dose Level Number examined Males_____ Females N-Ethyl Perfluorooctanesulfonamido (ppm) 0 3 30 100 3 20 100 5 0 0 10 5 0 0 10 Liver Vacuolation Minimal Slight Moderate 00 00 00 0 0 0 3 000 3 000 3 000 2 0 0 Hypertrophy Minimal Slight Moderate Moderately Severe 00 0 00 0 00 0 00 0 0 000 3 000 2 000 5 000 0 3 4 0 17 G 0 C 3 0 C*1 c Li e Table 1 Summary of Adjusted Survival Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN FATS DRAFT* PAGE: 1 GROUP AND DOSE LEVEL (PPM) WEEK: START 1 2 34 5 67 8 9 10 11 MALE 1 0 70/70 70/70 70/70 70/70 65/65 65/65 65/65 65/65 60/60 60/60 60/60 60/60 2 3 60/60 60/60 60/60 60/60 55/55 55/55 55/55 55/55 54/55 54/55 54/55 54/55 3 30 60/60 60/60 60/60 60/60 55/55 55/55 55/55 55/55 55/55 55/55 55/55 55/55 4 100 70/70 70/70 70/70 70/70 65/65 65/65 65/65 65/65 65/65 65/65 65/65 65/65 5 300 70/70 70/70 70/70 70/70 65/65 65/65 65/65 63/65 0 0 0 0 6 100 40/40 40/40 40/40 40/40 40/40 40/40 40/40 39/40 39/40 39/40 39/40 39/40 7 300 40/40 40/40 40/40 40/40 40/40 40/40 39/40 39/40 0 0 0 0 Table 1 Summary of Adjusted Survival Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DRAFT* PAGE: 2 GROUP AND DOSE LEVEL (PPM) WEEK: 12 13 14 15 16 17 18 19 20 21 22 23 0 2 3 3 30 4 100 O5 o 300 C*i 6 100 C-3 7 300 60/60 60/60 55/55 55/55 MALE 55/55 55/55 55/55 55/55 55/55 55/55 55/55 55/55 54/55 54/55 49/50 49/50 49/50 49/50 49/50 49/50 49/50 49/50 49/50 49/50 55/55 55/55 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 65/65 64/65 59/60 59/60 59/60 59/60 59/60 59/60 59/60 59/60 59/60 59/60 0 0 000 000 000 0 38/40 38/40 38/40 38/40 38/40 38/40 38/40 38/40 38/40 38/40 38/40 38/40 0 0 000 000 000 0 Table 1 Summary of Adjusted Survival Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS `DRAFT* PAGE: 3 GROUP AND DOSE LEVEL (PPM) WEEK: 24 25 26 27 28 29 30 31 32 33 34 35 cctroo MALE 0 55/55 55/55 55/55 55/55 55/55 55/55 55/55 55/55 55/55 55/55 55/55 55/55 2 3 49/50 49/50 49/50 49/50 49/50 49/50 49/50 49/50 49/50 49/50 49/50 48/50 3 30 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 4 100 59/60 59/60 59/60 59/60 59/60 59/60 59/60 59/60 59/60 59/60 59/60 59/60 5 300 0 0 0 0 0 0 0 0 0 0 0 0 6 100 38/40 38/40 38/40 38/40 38/40 38/40 38/40 38/40 38/40 38/40 38/40 38/40 7 300 0 0 0 0 0 0 0 0 0 0 0 0 Table 1 Summary of Adjusted Survival Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS `DRAFT* PAGE: 4 GROUP AND DOSE LEVEL (PPM) WEEK: 36 37 38 39 40 41 42 43 44 45 46 47 0 2 3 3 30 4 100 e o 5 300 6 u 100 7 300 55/55 55/55 55/55 55/55 MALE 55/55 55/55 55/55 55/55 54/55 54/55 54/55 54/55 48/50 48/50 48/50 48/50 48/50 48/50 48/50 48/50 48/50 48/50 48/50 48/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 59/60 59/60 59/60 59/60 59/60 59/60 59/60 59/60 59/60 58/60 58/60 58/60 00 000 000 0 000 38/40 38/40 38/40 38/40 38/40 38/40 38/40 37/40 37/40 37/40 37/40 37/40 0 0 000000 000 0 rs t,c CJ m Table 1 Summary of Adjusted Survival Data 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS `DRAFT* GROUP AND DOSE LEVEL (PPM) WEEK: 48 49 50 51 52 53 HALE 1 0 54/55 54/55 54/55 54/55 54/55 49/50 2 3 48/50 48/50 48/50 48/50 48/50 48/50 3 30 50/50 50/50 50/50 50/50 50/50 49/50 4 100 58/60 58/60 58/60 58/60 58/60 48/50 5 300 0 0 0 0 0 0 6 100 37/40 37/40 37/40 36/40 36/40 36/40 7 300 0 0 0 0 0 0 Covance 6329-212 3M T-6316.1 PAGE: 5 Table 1 Summary of Adjusted Survival Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DRAFT* PAGE: 6 GROUP AND DOSE LEVEL (PPM) WEEK: START 1 2 3 4 5 6 7 8 9 10 11 0 2 3 3 30 4 100 5 300 6 100 7 . 300 70/70 70/70 70/70 70/70 FEMALE 65/65 65/65 65/65 65/65 60/60 60/60 60/60 60/60 60/60 60/60 60/60 60/60 55/55 55/55 55/55 55/55 55/55 55/55 55/55 55/55 60/60 60/60 60/60 60/60 55/55 55/55 55/55 55/55 55/55 55/55 55/55 55/55 70/70 70/70 70/70 70/70 65/65 65/65 65/65 65/65 65/65 65/65 65/65 65/65 70/70 70/70 70/70 70/70 65/65 65/65 65/65 65/65 0 0 0 0 40/40 40/40 40/40 40/40 40/40 40/40 40/40 40/40 40/40 40/40 40/40 40/40 40/40 40/40 40/40 40/40 40/40 40/40 40/40 40/40 0 0 0 0 Vi c C-*- S-Jfc b Table 1 Summary of Adjusted Survival Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS `DRAFT* PAGE: 7 GROUP AND DOSE LEVEL (PPM) WEEK: 12 13 14 15 16 17 18 19 20 21 22 23 FEMALE 0 60/60 60/60 55/55 55/55 55/55 55/55 55/55 55/55 55/55 55/55 55/55 55/55 2 3 55/55 55/55 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 3 30 55/55 55/55 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 4 100 65/65 65/65 60/60 59/59 59/59 59/59 59/59 59/59 59/59 59/59 58/59 58/59 5 300 0 0 0 0 0 0 0 0 0 0 0 0 6 100 40/40 40/40 40/40 40/40 40/40 40/40 40/40 40/40 40/40 40/40 40/40 40/40 7 300 0 0 0 0 0 0 0 0 0 0 0 0 Tabla 1 Summary of Adjusted survival Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DRAFT* PAGE: 8 GROUP AND DOSE LEVEL (PPM) WEEK: 24 25 26 27 28 29 30 31 32 33 34 35 0 2 3 3 30 4 100 o5 300 c- 6 h* 100 o m7 300 55/55 55/55 55/55 55/55 FEMALE 55/55 55/55 55/55 55/55 55/55 55/55 55/55 55/55 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 58/59 58/59 58/59 58/59 58/59 58/59 58/59 58/59 58/59 58/59 58/59 58/59 000000000000 40/40 40/40 40/40 40/40 40/40 40/40 40/40 40/40 40/40 40/40 40/40 40/40 000000000000 C C- C.J Table 1 Summary of Adjusted Survival Data Covance 6329-212 3M T-6316.1 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS `DRAFT* PAGE: 9 GROUP AND DOSE LEVEL (PPM) WEEK: 36 37 38 39 40 41 42 43 44 45 46 47 FEMALE 0 55/55 55/55 55/55 55/55 55/55 55/55 55/55 55/55 55/55 55/55 55/55 54/55 2 3 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 3 30 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 4 100 58/59 58/59 58/59 58/59 58/59 58/59 58/59 58/59 58/59 58/59 58/59 58/59 5 300 0 0 0 0 0 0 0 0 0 0 0 0 6 100 40/40 40/40 40/40 40/40 40/40 40/40 40/40 40/40 40/40 40/40 40/40 40/40 7 300 0 0 0 0 0 0 0 0 0 0 0 0 Table 1 summary of Adjusted Survival Data 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DRAFT* GROUP AND DOSE LEVEL (PPM) WEEK: 48 49 50 51 52 53 1 0 2 3 3 30 4 100 o e5 Ci 300 6 100 O 7 300 54/55 54/55 54/55 54/55 FEMALE 54/55 49/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 50/50 49/50 58/59 58/59 58/59 58/59 58/59 48/49 000000 40/40 40/40 40/40 40/40 40/40 40/40 000000 Covance 6329-212 3M T-6316.1 PAGE: 10 Table 2 Summary of Adjusted Survival Data `DRAFT* 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 1 GROUP AND DOSE LEVEL (PPM) WEEK: START 1 2 3 4 5 6 7 8 9 10 11 HALE 8 0 70/70 70/70 70/70 70/70 65/65 65/65 65/65 65/65 65/65 65/65 65/65 64/65 9 1 70/70 70/70 70/70 70/70 65/65 65/65 65/65 65/65 65/65 65/65 65/65 65/65 8 0 o 9 1 Ci H* i M 70/70 70/70 70/70 70/70 FEMALE 65/65 65/65 65/65 65/65 64/65 64/65 64/65 64/65 70/70 70/70 70/70 70/70 65/65 65/65 65/65 65/65 65/65 65/65 65/65 65/65 c Table 2 Summary of Adjusted Survival Data DRAFT* 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 2 GROUP AND DOSE LEVEL (PPM) WEEK: 12 13 14 15 16 17 18 19 20 21 22 23 oo o 00 o MALE 64/65 64/65 59/60 59/60 59/60 59/60 59/60 59/60 59/60 59/60 59/60 59/60 9 1 65/65 65/65 60/60 60/60 60/60 60/60 60/60 60/60 60/60 60/60 60/60 60/60 FEMALE 64/65 64/65 59/60 59/60 59/60 59/60 59/60 59/60 59/60 59/60 59/60 59/60 9 1 65/65 65/65 60/60 60/60 60/60 60/60 60/60 60/60 60/60 60/60 60/60 60/60 Table 2 Summary of Adjusted Survival Data `DRAFT* 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 3 GROUP AND DOSE LEVEL (PPM) WEEK: 24 25 26 27 28 29 30 31 32 33 34 35 MALE 8 0 59/60 59/60 59/60 59/60 59/60 59/60 59/60 59/60 59/60 59/60 59/60 59/60 9 1 60/60 60/60 60/60 60/60 60/60 60/60 60/60 60/60 60/60 60/60 60/60 60/60 8 0 o9 1 59/60 59/60 59/60 59/60 FEMALE 59/60 59/60 58/60 58/60 58/60 58/60 58/60 58/60 60/60 60/60 60/60 60/60 60/60 60/60 60/60 60/60 60/60 60/60 60/60 60/60 Table 2 Summary of Adjusted Survival Data DRAFT* 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 4 GROUP AND DOSE LEVEL (PPM) WEEK: 36 37 38 39 40 41 42 43 44 45 46 47 MALE 3 0 59/60 59/60 59/60 59/60 59/60 59/60 59/60 59/60 59/60 59/60 59/60 59/60 9 1 60/60 60/60 60/60 60/60 60/60 60/60 58/60 58/60 58/60 58/60 58/60 58/60 8 0 e e9 c--. 1 h 58/60 58/60 58/60 58/60 FEMALE 58/60 58/60 58/60 58/60 58/60 58/60 58/60 58/60 60/60 60/60 60/60 60/60 60/60 60/60 60/60 60/60 60/60 60/60 60/60 60/60 Table 2 Summary of Adjusted Survival Data DRAFT* 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS GROUP AND DOSE LEVEL (PPM) WEEK: 48 49 50 51 52 53 MALE 8 0 59/60 59/60 59/60 58/60 58/60 48/50 9 1 58/60 58/60 58/60 58/60 58/60 48/50 8 0 o9 o1 Ca 57/60 57/60 56/60 56/60 FEMALE 56/60 46/50 60/60 60/60 59/60 58/60 58/60 48/50 in Covance 6329-228 3M T-6316.1 PAGE: 5 DAYS 1-371 CATEGORY KEYWORD QUALIFIER *** TOP OF LIST *** APPEARANCE BENT TAIL CONVULSIONS DEHYDRATED HUNCHED MALOCCLUSION MISSING TAIL-PROXIMAL <r% TEETH PARALYSIS O ' LIMB-FRONT-LEFT SWOLLEN LIMB-HIND-LEFT s LIMB-HIND-RIGHT MOUTH C l NOSE PAW-HIND-LEFT VENTRAL-ABDOMINAL THIN BEHAVIOR HYPOACTIVE RECUMBENT LATERAL STERNAL Table 3 Summary of Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DRAFT* SEX: GROUP : DOSE: NUMBER: NUMBER OF ANIMALS AFFECTED -- MALE1234 5 67 0 3 30 100 300 100 300 70 60 60 70 70 40 40 0000000 00002 00 0000001 00000 10 3 3 5 19 2 6 2 0000000 1013 0 00 0001010 1000000 0100000 0001010 0000000 0000000 0000010 0301111 0000111 0000010 1000000 Covance 6329-212 3M T-6316.1 PAGE: 1 DAYS 1-371 CATEGORY KEYWORD QUALIFIER BEHAVIOR SENSITIVE TO TOUCH VOCALIZATION DISCHARGE GENITAL RED IN COLOR 6 C-5 ORAL RED IN COLOR CLEAR I"* NASAL RED IN COLOR UNKNOWN RED IN COLOR EXCRETION DISCOLORED URINE RED IN COLOR ORANGE IN COLOR FEW FECES LIQUID FECES NO FECES NONFORMED FECES Table 3 Summary of Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DRAFT* SEX: GROUP: DOSE: NUMBER: NUMBER OF ANIMALS AFFECTED --12 03 -- MALE34 5 67 30 100 300 100 300 70 60 60 70 70 40 40 1000000 0000100 1110020 0100001 0000010 0002140 0000100 1000000 1000000 1300021 0000010 1000010 5553020 Covance 6329-212 3M T-6316.1 PAGE: 2 Table 3 Summary of Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS *DRAFT* DAYS 1-371 CATEGORY KEYWORD QUALIFIER SEX: GROUP: DOSE: NUMBER: NUMBER OF ANIMALS AFFECTED -MALE---12 34 5 67 0 3 30 100 300 100 300 70 60 60 70 70 40 40 EYES CLEAR DISCHARGE EYE-RIGHT EYES CLOUDY DISCHARGE EYE-RIGHT OPAQUE f.; EYE-RIGHT RED DISCHARGE EYE-LEFT EYE-RIGHT EYES SQUINTED PERI-ORBITAL HOUSING,FOOD,WATER & MAINT. TEETH CUT RESPIRATION AUDIBLE IRREGULAR LABORED SKIN & PELAGE ALOPECIA 0000000 1000000 0000000 0000000 1020030 0101000 2101030 0000000 1000000 0 1 0 10 00 0100020 0 1 0 01 10 0000000 Covance 6329-212 3M T-6316.1 PAGE: 3  fV--<-, (3 DAYS 1-371 CATEGORY KEYWORD QUALIFIER SKIN & PELAGE BROWN HAIR COAT PERINEAL AREA SCROTUM VENTRAL-ABDOMINAL BLUE SKIN VENTRAL-ABDOMINAL- RIGHT COLD TO TOUCH ENTIRE BODY PALE EARS entire body RED HAIR COAT HEAD-ENTIRE HEAD-CRANIAL LIMBS-ALL VENTRAL-ABDOMINAL ROUGH HAIR COAT SCALY SKIN TAIL-DISTAL SORE/SCAB AXILLARY REGION-RIGHT DORSAL DORSAL-CERVICAL DORSAL-CERVICAL-LEFT DORSAL-CERVICAL-RIGHT Table 3 Summary of Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DRAFT* SEX: GROUP: DOSE: NUMBER: NUMBER OF ANIMALS AFFECTED -- MALE1234 5 67 0 3 30 100 300 100 300 70 60 60 70 70 40 40 1100000 0001000 0001010 0000000 0000100 0000000 1100100 0200100 0000000 0100000 0100001 0101010 0000000 0000000 1000000 1000000 0000000 0010000 Covance 6329-212 3M T-6316.1 PAGE: 4 Table 3 Summary of Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS `DRAFT* DAYS 1-371 CATEGORY KEYWORD QUALIFIER SEX: GROUP: DOSE: NUMBER: NUMBER OF ANIMALS AFFECTED --MALE123 4 567 0 3 30 100 300 100 300 70 60 60 70 70 40 40 SKIN & PELAGE SORE/SCAB EAR-RIGHT HEAD-ENTIRE HEAD-CRANIAL HEAD-MAXILLARY-RIGHT LATERAL-ABDOMINAL-RIGHT LATERAL-CERVICAL-LEFT o LATERAL-CERVICAL-RIGHT LIMB-HIND-LEFT LIMB-HIND-RIGHT LIMBS-HIND H - MOUTH Ci PAW-HIND-LEFT PAW-HIND-RIGHT O PAWS-FRONT PAWS-HIND SHOULDER-LEFT SHOULDER-RIGHT TAIL VENTRAL-ABDOMINAL VENTRAL-ABDOMINAL-LEFT VENTRAL-ABDOMINAL-RIGHT VENTRAL-CERVICAL VENTRAL-CERVICAL-LEFT VENTRAL-CERVICAL-RIGHT 0010000 0000001 0100000 0011000 0100000 1000000 1000000 0010000 0100000 1000000 0000000 1111010 0211010 0000000 1101010 0210000 0000000 1100000 0011010 0100000 0000000 1100110 0010000 0010000 Covance 6329-212 3M T-6316.1 PAGE: 5 003151 DAYS 1-371 CATEGORY KEYWORD QUALIFIER SKIN * PELAGE YELLOW HAIR COAT MASS(ES) AXILLARY REGION-LEFT WEEK 26 WEEK 33 WEEK 43 WEEK 47 AXILLARY REGION-RIGHT WEEK 31 WEEK 35 WEEK 38 WEEK 45 WEEK 47 WEEK 48 WEEK 52 DORSAL WEEK 49 HEAD-MAXILLARY-RIGHT WEEK 17 WEEK 48 WEEK 51 Table 3 Summary of clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS `DRAFT* SEX: GROUP: DOSE: NUMBER: NUMBER OF ANIMALS AFFECTED -MALE1234 567 0 3 30 100 300 100 300 70 60 60 70 70 40 40 0000011 0000000 0000000 1000000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 0000000 1000000 0000000 0000000 0100000 Covance 6329-212 3M T-6316.1 PAGE: 6 DAYS 1-371 CATEGORY KEYWORD QUALIFIER MASS(ES) LIMB-HIND-LEFT WEEK 25 MOUTH WEEK 20 SHOULDER-LEFT WEEK 40 VENTRAL-ABDOMINAL WEEK 33 WEEK 35 WEEK 40 WEEK 44 WEEK 49 WEEK 51 VENTRAL-ABDOMINAL- LEFT WEEK 21 WEEK 22 WEEK 37 WEEK 45 WEEK 46 WEEK 49 WEEK 51 WEEK 53 VENTRAL-ABDOMINAL-RIGHT WEEK 29 WEEK 32 Table 3 Summary of Clinical observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS `DRAFT* SEX: GROUP: DOSE: NUMBER: NUMBER OF ANIMALS AFFECTED -MALE12 34 5 67 0 3 30 100 300 100 300 70 60 ' 60 70 70 40 40 0000000 1000000 0010000 0000000 0000000 0000000 0000000 0000000 0000000 0100000 0000000 0001000 0000000 0000000 0000000 0100000 0000000 0001000 0000000 Covance 6329-212 3M T-S316.1 PAGE: 7 esTPoo DAYS 1-371 CATEGORY KEYWORD QUALIFIER MASS (ES) VENTRAL-ABDOMINAL-RIGHT WEEK 36 WEEK 38 WEEK 40 WEEK 49 WEEK 51 WEEK 53 VENTRAL-CERVICAL-LEFT WEEK 43 WEEK 46 WEEK 48 VENTRAL-CERVICAL-RIGHT WEEK 43 VENTRAL-THORACIC WEEK 42 WART LIKE LESION(S) DORSAL WEEK 47 WEEK 51 EAR-LEFT WEEK 47 MOUTH WEEK 21 WEEK 23 Table 3 Summary of Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DRAFT* SEX: GROUP : DOSE: NUMBER: NUMBER OF ANIMALS AFFECTED -- MALE12 34 567 0 3 30 100 300 100 300 70 60 60 70 70 40 40 0000000 0001000 0000000 0000000 0000000 0000000 00 0 0 0 00 0000000 0000000 0000000 1000000 0100000 1000000 01 00 0 00 0000000 0000000 Covance 6329-212 3M T-6316.1 PAGE: 8 DAYS 1-371 CATEGORY KEYWORD QUALIFIER WART LIKE LESION(S) MOUTH WEEK 31 WEEK 52 PAW-HIND-RIGHT WEEK 34 e SHOULDER-LEFT e WEEK 37 TAIL c,< WEEK 19 WEEK 22 VENTRAL-THORACIC WEEK 46 END OF LIST Table 3 Sununary of Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DRAFT* SEX: GROUP: DOSE: NUMBER: NUMBER OF ANIMALS AFFECTED --MALE1234 567 0 3 30 100 300 100 300 70 60 60 70 70 40 40 0000000 0000000 0100000 0010000 0100000 0120000 0000000 Covance 6329-212 3M T-6316.1 PAGE: 9 ssiroo DAYS 1-371 CATEGORY KEYWORD QUALIFIER *** TOP OF LIST *** APPEARANCE BENT TAIL CONVULSIONS DEHYDRATED HUNCHED MALOCCLUSION MISSING TAIL-PROXIMAL TEETH PARALYSIS LIMB-FRONT-LEFT SWOLLEN LIMB-HIND-LEFT LIMB-HIND-RIGHT MOUTH NOSE PAW-HIND-LEFT VENTRAL-ABDOMINAL THIN BEHAVIOR HYPOACTIVE RECUMBENT LATERAL STERNAL Table 3 Summary of Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ` DRAFT* SEX: GROUP: DOSE: NUMBER: NUMBER OF ANIMALS AFFECTED -FEMALE--1234 5 67 0 3 30 100 300 100 300 70 60 60 70 70 40 40 0000010 0010000 0000000 0001000 5 9 6 13 0 6 0 0000010 0 0 0 0 0 00 0000000 0000000 0000000 0000000 0000010 0001000 0000000 1001010 0010000 0000000 0000000 Covance 6329-212 3M T-6316.1 PAGE: 10 DAYS 1-371 CATEGORY KEYWORD QUALIFIER BEHAVIOR SENSITIVE TO TOUCH VOCALIZATION DISCHARGE o GENITAL c Ci RED IN COLOR ORAL RED IN COLOR h CLEAR in NASAL RED IN COLOR a UNKNOWN RED IN COLOR EXCRETION DISCOLORED URINE RED IN COLOR ORANGE IN COLOR FEW FECES LIQUID FECES NO FECES NONFORMED FECES Table 3 Summary of Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DRAFT* SEX: GROUP: DOSE: NUMBER: NUMBER OF ANIMALS AFFECTED -FEMALE--1234 5 67 0 3 30 100 300 100 300 70 60 60 70 70 40 40 0001000 0000000 00 0 1 02 0 0001000 0000000 1113010 0000000 0000000 0000000 0011000 0000000 0001010 2000000 Covance 6329-212 3M T-6316.1 PAGE: 11 6(T-> C*-* VI DAYS 1-371 CATEGORY KEYWORD QUALIFIER EYES CLEAR DISCHARGE EYE-RIGHT EYES CLOUDY DISCHARGE EYE-RIGHT OPAQUE EYE-RIGHT RED DISCHARGE EYE-LEFT EYE-RIGHT EYES SQUINTED PERI-ORBITAL HOUSING, FOOD, WATER & MAINT. TEETH CUT RESPIRATION AUDIBLE IRREGULAR LABORED SKIN & PELAGE ALOPECIA Table 3 Summary of Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS `DRAFT* SEX: GROUP: DOSE: NUMBER: NUMBER OF ANIMALS AFFECTED -FEMALE--12345 67 0 3 30 100 300 100 300 70 60 60 70 70 40 40 0000010 0000000 1000000 0000010 1210010 0100020 0111000 0010000 0000010 0012000 0000000 00l0000 0002010 Covance 6329-212 3M T-6316.1 PAGE: 12 Table 3 Summary of Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS *DRAFT* DAYS 1-371 CATEGORY KEYWORD QUALIFIER SEX: GROUP : DOSE: NUMBER: NUMBER OF ANIMALS AFFECTED -FEMALE--1234 5 67 0 3 30 100 300 100 300 70 60 60 70 70 40 40 SKIN t PELAGE BROWN HAIR COAT PERINEAL AREA SCROTUM VENTRAL-ABDOMINAL BLUE SKIN r s VENTRAL-ABDOMINAL-RIGHT COLD TO TOUCH ENTIRE BODY C- PALE EARS ENTIRE BODY fc1 RED HAIR COAT m HEAD-ENTIRE HEAD-CRANIAL LIMBS-ALL VENTRAL-ABDOMINAL ROUGH HAIR COAT SCALY SKIN TAIL-DISTAL SORE/SCAB AXILLARY REGION-RIGHT DORSAL DORSAL-CERVICAL DORSAL-CERVICAL-LEFT DORSAL-CERVICAL-RIGHT 0010000 0000000 0001000 0010000 1000000 0100000 1101010 0000000 0000010 0000000 0000000 0002040 0001000 0100000 0001000 0000000 1000000 0000000 Covance 6329-212 3M T-6316.1 PAGE: 13 DAYS 1-371 CATEGORY KEYWORD QUALIFIER o f* m SKIN & PELAGE SORE/SCAB EAR-RIGHT HEAD-ENTIRE HEAD-CRANIAL HEAD-MAXI LLARY-RIGHT LATERAL-ABDOMINAL-RIGHT LATERAL-CERVICAL-LEFT LATERAL- CERVICAL- RIGHT LIMB-HIND-LEFT LIMB-HIND-RIGHT LIMBS-HIND MOUTH PAW-HIND-LEFT PAW-HIND-RIGHT PAWS-FRONT PAWS-HIND SHOULDER-LEFT SHOULDER- RIGHT TAIL VENTRAL-ABDOMINAL VENTRAL-ABDOMINAL-LEFT VENTRAL-ABDOMINAL-RIGHT VENTRAL-CERVICAL VENTRAL-CERVICAL-LEFT VENTRAL-CERVICAL-RIGHT Table 3 Summary of Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS *DRAFT* SEX: GROUP: DOSE: NUMBER: NUMBER OF ANIMALS AFFECTED -- FEMALE--1234 567 0 3 30 100 300 100 300 70 60 60 70 70 40 40 0 0 0 0 00 0 00 000 00 00000 0 0 0000 011 0 0 0 0 00 0 0100 00 0 0 0 0 0 00 0 0000000 0000000 0000000 0 0 0 0 0 0 0 0 0 0 10 0 0 001 100 1000 1011 00 0 000 00000 0 2 1 20 10 10 0000 0 10 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 00 00 10 0 0000010 0 0 0 0 0 0 0 0 0 0 0 0 0 0 Covance 6329-212 3M T-6316.1 PAGE: 14 ooaiG o DAYS 1-371 CATEGORY KEYWORD QUALIFIER SKIN & PELAGE YELLOW HAIR COAT M A S S (E S } AXILLARY REGION-LEFT WEEK 26 WE E K 33 W EEK 43 W EEK 47 AXILLARY REGION-RIGHT WEEK 31 WEEK 35 WEEK 38 WEEK 45 WEEK 47 WEEK 48 W E E K 52 DORSAL W EEK 49 HEAD-MAXILLARY-RIGHT W E E K 17 WEEK 48 W EEK 51 Table 3 Summary of Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DRAFT* SEX: GROUP: DOSE: NUMBER: NUMBER OF ANIMALS AFFECTED - F E M A L E ---12 3 4 5 67 0 3 30 100 300 100 300 70 60 60 70 70 40 40 0 00 1000 10 00000 00 10000 00 10000 01 00000 00 10000 01 00000 22 00000 00 01000 1 0 00000 00 01000 0021000 0 0 00000 00 01000 00 01000 0000000 Covance 6329-212 3M T-6316.1 PAGE: 15 DAYS 1-371 CATEGORY KEYWORD QUALIFIER M A S S (ES) LIMB-HIND-LEFT WEEK 25 MOUTH WEEK 20 SHOULDER-LEFT WEEK 40 VENTRAL-ABDOMINAL WEEK 33 WEEK 35 WEEK 40 WEEK 44 WEEK 49 WEEK 51 VENTRAL-ABDOMINAL- LEFT WEEK 21 WEEK 22 WEEK 37 WEEK 45 WEEK 46 WEEK 49 WEEK 51 WEEK 53 VENTRAL-ABDOMINAL-RIGHT WEEK 29 Table 3 Summary of Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS `DRAFT* SEX: GROUP : DOSE: NUMBER: NUMBER OF ANIMALS AFFECTED -FEMALE--12 3 4 5 67 0 3 30 100 300 100 300 70 60 60 70 70 40 40 1000000 0000000 0000000 0001000 02 0 0 01 0 0100000 0000010 0100000 1100000 0000000 0010000 0000000 1000000 0100010 0100000 0000010 0010000 0000000 Covance 6329-212 3M T-6316.1 PAGE: 16 DAYS 1-371 CATEGORY KEYWORD QUALIFIER MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 36 WEEK 38 WEEK 40 WEEK 49 WEEK 51 WEEK 53 VENTRAL-CERVICAL-LEFT WEEK 43 WEEK 46 WEEK 48 VENTRAL- CERVICAL-RIGHT WEEK 43 VENTRAL-THORACIC WEEK 42 WART LIKE LESION(S) DORSAL WEEK 47 WEEK 51 EAR-LEFT WEEK 47 MOUTH WEEK 21 WEEK 23 Table 3 Summary of Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DRAFT* SEX: GROUP: DOSE: NUMBER: NUMBER OF ANIMALS AFFECTED -FEMALE--12 34 567 0 3 30 100 300 100 300 70 60 60 70 70 40 40 1000000 0010000 00 10 000 0100000 1011010 0010000 0000010 0010000 0000010 0001000 0000000 0000000 0000000 0000000 1000000 0001000 Covance 6329-212 3M T-6316.1 PAGE: 17 DAYS 1-371 CATEGORY KEYWORD QUALIFIER WART LIKE LESION(S) MOUTH WEEK 31 WEEK 52 PAW-HIND-RIGHT WEEK 34 o SHOULDER-LEFT WEEK 37 o TAIL C-< WEEK 19 WEEK 22 M VENTRAL-THORACIC a CJ WEEK 46 *** END OF LIST *** Table 3 Summary of Clinical Observations 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DRAFT* SEX: GROUP: DOSE: NUMBER: NUMBER OF ANIMALS AFFECTED -FEMALE--12 34 567 0 3 30 100 300 100 300 70 60 60 70 70 40 40 0001000 0100000 0000000 0000000 0000000 0000000 0010000 Covance S329-212 3M T-6316.1 PAGE: 18 s DAYS 1-371 CATEGORY KEYWORD QUALIFIER *** TOP OF LIST *** APPEARANCE HUNCHED MALOCCLUSION MISSING TEETH PARALYSIS LIMB-FRONT-LEFT SWOLLEN O MOUTH THIN o C-* H- BEHAVIOR AGGRESSIVE UNSPECIFIED cn MODERATE ATftXTC it* <UNSPECIFIED HYPOACTIVE UNSPECIFIED SENSITIVE TO TOUCH COLD TO TOUCH ENTIRE BODY DISCHARGE GENITAL RED IN COLOR Table 4 Summary of Clinical Observations DRAFT* 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS NUMBER OF ANIMALS AFFECTED SEX: GROUP: DOSE: -- MALE-- 89 01 -FEMALE' 89 01 NUMBER: 70 70 70 70 0100 10 4 13 8 0100 0100 0010 0121 0100 0100 0001 1000 0101 1000 0201 Covance 6329-228 3M T-6316.1 PAGE: 1 DAYS 1-371 CATEGORY KEYWORD QUALIFIER DISCHARGE CLEAR ORAL MODERATE NASAL RED IN COLOR MUCOID FROM RECTUM EXCRETION o FEW FECES NO FECES O ' NONFORMED FECES C EYES DISPLACED PUPIL s EYE-RIGHT RED DISCHARGE o EYE-LEFT EYE-RIGHT EYES SQUINTED PERI-ORBITAL RESPIRATION AUDIBLE LABORED Table 4 Summary of Clinical Observations DRAFT* 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS NUMBER OF ANIMALS AFFECTED SEX: GROUP: DOSE: --MALE-- 89 01 -FEMALE 89 01 NUMBER: 70 70 70 70 0 10 0 0031 0 0 10 10 10 0 1 0 0 67 0 0 0 0 10 00 12 11 13 1111 0 00 1 10 10 0 0 10 Covance 6329-228 3M T-6316.1 PAGE: 2 DAYS 1-371 CATEGORY KEYWORD QUALIFIER c to .Ht CD /u9S SKIN & PELAGE ALOPECIA BLUE SKIN VENTRAL-ABDOMINAL-LEFT PALE ENTIRE BODY RED HAIR COAT DORSAL HEAD-ENTIRE RED SKIN TAIL SORE/SCAB DORSAL DORSAL-CERVICAL HEAD-ENTIRE HEAD-CRANIAL HEAD-MAXILLARY-LEFT LATERAL-CERVICAL-LEFT LUMBAR-RIGHT MOUTH SCROTUM SHOULDER-RIGHT VENTRAL-ABDOMINAL VENTRAL-ABDOMINAL-LEFT VENTRAL-ABDOMINAL-RIGHT VENTRAL-CERVICAL Table 4 Summary of Clinical Observations DRAFT* 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANES0LFONAMIDO ETHANOL IN RATS NUMBER OF ANIMALS AFFECTED SEX: GROUP: DOSE: -MALE- 89 01 -FEMALE- 89 01 NUMBER: 70 70 70 70 0010 0001 0010 0001 2010 1000 0001 1 100 001 0 0001 0010 0001 1 0 00 01 00 1 000 0010 21 00 2 1 00 1 1 00 0010 Covance 6329-228 3M T-6316.1 PAGE: 3  9 T ro o DAYS 1-371 CATEGORY KEYWORD QUALIFIER SKIN A PELAGE YELLOW HAIR COAT MASS(ES) AXILLARY REGION-LEFT WEEK 42 WEEK 45 WEEK 48 WEEK 51 AXILLARY REGION-RIGHT WEEK 28 WEEK 39 WEEK 40 WEEK 42 INGUINAL REGION-LEFT WEEK 48 INGUINAL REGION-RIGHT WEEK 35 WEEK 40 WEEK 42 LIMB-FRONT-RIGHT WEEK 35 Table 4 Summary of Clinical Observations `DRAFT* 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS NUMBER OF ANIMALS AFFECTED SEX: GROUP: DOSE: -- MALE-- 89 01 -FEMALE 89 01 NUMBER: 70 70 70 70 0120 0001 0010 0001 0002 0001 0001 0010 0011 0001 0001 0001 0001 0001 Covanee 6329-228 SM T-6316.1 PAGE: 4 o f; Ct m DAYS 1-371 CATEGORY KEYWORD QUALIFIER MASS(ES) PAW-HIND-RIGHT WEEK 42 SHOULDER-LEFT WEEK 38 SHOULDER-RIGHT WEEK 19 VENTRAL*ABDOMINAL WEEK 32 WEEK 35 WEEK 40 WEEK 44 WEEK 45 WEEK 50 VENTRAL-ABDOMINAL-LEFT WEEK 28 WEEK 34 WEEK 40 WEEK 44 WEEK 46 WEEK 49 WEEK 50 WEEK 51 VENTRAL-ABDOMINAL-RIGHT WEEK 28 WEEK 33 Table 4 Summary of Clinical Observations DRAFT* 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS NUMBER OF ANIMALS AFFECTED SEX: GROUP: DOSE: -- MALE-- 89 01 -FEMALE 89 01 NUMBER: 70 70 70 70 0001 0010 0010 1000 0001 0100 0002 0100 0011 1000 0001 1000 0021 0100 0020 0021 0 02 0 1000 1000 Covance 6329-228 3M T-6316.1 PAGE: 5 DAYS 1-371 CATEGORY KEYWORD QUALIFIER MASS(ES) VENTRAL-ABDOMINAL-RIGHT WEEK 40 WEEK 44 WEEK 45 WEEK 48 WEEK 51 WEEK 53 VENTRAL-CERVICAL e WEEK 36 WEEK 46 VENTRAL-CERVICAL-LEFT 4 WEEK 31 WEEK 38 a WART LIKE LESION(S) DORSAL WEEK 41 NOSE WEEK 46 VENTRAL-ABDOMINAL WEEK 20 *** END OF LIST *** Table 4 Summary of Clinical Observations `DRAFT* 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS NUMBER OF ANIMALS AFFECTED SEX: GROUP: DOSE: -- MALE-- 89 01 -FEMALE 89 01 NUMBER: 70 70 70 70 0001 0001 0001 0100 0010 0020 0010 0020 0 0- 1 0 0001 1000 0010 1000 Covance 6329-228 3M T-6316.1 PAGE: 6 Table 5 Summary of Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DRAFT* SEX: --- MALE---- GROUP: 1 2 3 4 5 6 7 DOSE: 0 3 30 100 300 100 300 WEEK UNITS: PPM PPM PPM PPM PPM PPM PPM 1N 70 MEAN 261 S.D. 20.4 60 260 19.9 60 260 20.5 70 258 19.2 69 257 19.0 40 254 17.8 40 256 19.2 2N 70 MEAN 308 S.D. 23.1 60 307 22.5 60 302 24.5 70 298* 21.7 70 288* 23.0 40 296* 20.0 40 286* 21.2 3N 70 MEAN 350 S.D. 25.7 4N 70 MEAN 387 S.D. 29.0 60 351 26.6 60 389 29.9 60 344 29.9 60 379 34.8 70 337* 25.3 70 367* 28.3 70 318* 25.9 70 338* 28.8 40 334* 24.2 40 364* 27.8 40 313* 24.0 40 334* 27.3 5N 65 55 55 65 64 40 40 MEAN 417 416 404 393* 355* 391* 351* S.D. 31.5 31.5 38.2 30.5 31.8 32.5 30.3 O . 6 N 65 55 55 65 65 40 40 MEAN 444 444 431 418* 370* 416* 366* S.D. 33.6 33.9 40.5 33.6 35.3 36.2 33.1 7N 65 55 55 65 65 40 39 MEAN 467 467 450 436* 380* 427* 374* S.D. 37.0 37.1 42.6 35.5 37.6 42.8 33.9 Covance 6329-212 3M T-6316.1 PAGE: 1 SEX: GROUP: DOSE: WEEK UNITS: 1 0 PPM 8N 65 MEAN 483 S.D. 39.5 9N 60 MEAN 504 S.D. 43.4 10 N 60 MEAN 521 S.D. 45.1 11 N 60 MEAN 532 S.D. 45.4 12 N 60 MEAN 543 O S.D. 46.8 13 N 60 c< MEAN 553 S.D. 47.7 14 N 60 MEAN 563 S.D. 50.1 Table 5 Summary of Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DRAFT* --- MALE---2345 67 3 30 100 300 100 300 PPM PPM PPM PPM PPM PPM 55 480 40.0 54 507 40.2 54 524 41.1 54 537 43.3 54 550 44.6 54 559 48.3 54 567 48.0 55 464* 43.4 55 483* 45.9 55 497* 49.3 55 510* 49.8 55 522 51.5 55 531 52.7 55 538* 54.5 65 449* 39.4 65 465* 40.9 65 477* 41.8 65 486* 43.7 65 496* 46.9 65 504* 51.2 64 513* 45.3 63 386* 42.4 0 0 0 0 0 0 39 448* 44.8 39 464* 51.0 39 473* 57.6 39 484* 62.4 39 496* 64.9 38 515* 44.9 38 522* 45.5 39 384* 38.5 0 0 0 0 0 0 Covance 6329-212 3M T-6316.1 PAGE: 2 Table 5 Summary of Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS `DRAFT* SEX: -------------------------------------- MALE GROUP: 1 2 3 4 DOSE: 0 3 30 100 WEEK UNITS: PPM PPM PPM PPM 15 N 55 MEAN 569 S.D. 51.8 16 N 55 MEAN 578 S.D. 52.9 17 N 55 MEAN 590 S.D. 55.0 21 N 55 MEAN 619 S.D. 58.8 25 N 55 MEAN 644 S.D. 62.8 29 N 55 MEAN 663 S.D. 67.1 33 N 55 MEAN 678 S.D. 71.5 49 574 50.9 49 585 52.3 49 595 54.0 49 625 58.3 49 651 64.2 49 668 69.6 49 682 72.0 50 540* 51.9 50 547* 53.1 50 557* 53.4 50 582* 57.0 50 603* 62.1 50 618* 66.5 50 634* 70.2 59 519* 45.8 59 528* 45.6 59 536* 46.4 59 555* 51.4 59 572* 51.3 59 590* 54.7 59 599* 57.5 5 300 PPM 0 0 0 0 0 0 0 6 100 PPM 38 528* 47.5 38 536* 48.4 38 546* 50.2 38 567* 55.5 38 582* 60.7 38 602* 64.8 38 612* 64.5 7 300 PPM 0 0 0 0 0 0 0 Covance 6329-212 3M T-6316.1 PAGE: 3 000173 SEX: GROUP: DOSE: WEEK UNITS: 1 0 PPM 37 N 55 MEAN 694 S.D. 77.1 41 N 55 MEAN 700 S.D. 71.4 45 N 54 MEAN 723 S.D. 76.2 49 N 54 MEAN 741 S.D. 78.3 53 N 54 MEAN 762 S.D. 81.9 Table 5 Summary of Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DRAFT* 2 3 PPM 48 701 75.1 48 714 75.2 48 740 82.8 48 758 91.5 48 777 95.0 --- MALE----34 30 100 PPM PPM 50 646* 74.0 50 653* 77.1 50 678* 82.6 50 698* 87.5 50 716* 92.5 59 610* 57.6 59 619* 60.1 58 639* 64.9 58 654* 68.7 58 672* 68.4 5 300 PPM 0 0 0 0 0 6 100 PPM 38 623* 67.4 38 632* 68.2 37 650* 76.4 37 666* 80.4 36 688* 86.3 7 300 PPM 0 0 0 0 0 Covance 6329-212 3M T-6316.1 PAGE: 4 Table 5 Summary of Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS `DRAFT* SEX: GROUP: 1 2 3 4 5 67 DOSE: 0 3 30 100 300 100 300 WEEK UNITS: PPM PPM PPM PPM PPM PPM PPM 1N 70 MEAN 165 S.D. 13.2 60 163 13.3 60 165 12.9 70 164 12.1 70 164 12.6 39 164 12.5 40 163 12.0 2N 70 MEAN 188 S.D. 16.9 60 185 14.7 60 185 14.4 70 181* 13.5 70 172* 13.3 40 181 14.1 40 175* 11.7 3N 70 60 60 70 70 40 40 o MEAN 211 205 205 200* 182* 202 186* o S.D. 20.8 18.5 16.5 15.5 15.1 15.8 12.2 4N 70 MEAN 229 S.D. 22.3 60 224 20.2 60 222 18.3 70 215* 17.1 70 191* 15.2 40 214* 15.9 40 196* 12.7 4* 5 N 65 55 55 65 65 40 40 MEAN 244 236 233* 225* 196* 223* 200* S.D. 25.4 21.2 19.9 18.6 16.4 16.7 13.3 6N 65 MEAN 258 S.D. 28.3 55 246 22.7 55 245 21.2 65 235* 19.4 65 202* 16.6 40 234* 17.3 40 206* 14.4 7N 65 MEAN 267 S.D. 30.0 55 256 23.8 55 253 22.7 65 239* 19.6 65 205* 16.5 40 240* 17.4 40 210* 13.2 Covance 6329-212 3M T-6316.1 PAGE: 5 o o r- A sT 01 WEEK SEX: GROUP: DOSE: UNITS: 8N MEAN S.D. 9N MEAN S.D. 10 N MEAN S.D. 11 N MEAN S.D. 12 N MEAN S.D. 13 N MEAN S.D. 14 N MEAN S.D. 1 0 PPM 65 274 30.4 60 283 32.1 60 290 33.6 60 295 35.1 60 302 36.6 60 306 36.3 60 308 36.3 Table 5 Summary of Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS `DRAFT* -- FEMALE--2 34 5 67 3 30 100 300 100 300 PPM PPM PPM PPM PPM PPM 55 261 24.9 55 271 25.6 55 277 27.3 55 281 28.7 55 284 28.6 55 289 27.5 55 292* 27.9 55 258* 23.6 55 268* 24.1 55 273* 25.1 55 275* 26.3 55 280* 26.7 55 284* 26.6 55 286* 27.7 65 246* 20.7 65 250* 20.7 65 254* 20.3 65 256* 21.8 65 258* 21.4 65 260* 21.7 65 262* 22.3 65 212* 17.1 0 0 0 0 0 0 40 243* 19.4 40 250* 18.9 40 253* 21.5 40 255* 19.9 40 259* 20.8 40 261* 20.2 40 264* 20.8 40 213* 14.9 0 0 0 0 0 0 Covance 6329-212 3M T-6316.1 PAGE: 6 SEX: GROUP: DOSE: WEEK UNITS: 1 0 PPM 15 N 55 MEAN 310 S.D. 36.0 16 N 55 MEAN 316 S.D. 36.8 17 N 55 MEAN 320 S.D. 37.9 21 N 55 c. MEAN 334 S.D. 41.9 25 N 55 MEAN 348 S.D. 47.6 29 N 55 MEAN 361 S.D. 51.9 33 N 55 MEAN 375 S.D. 55.1 Table 5 Summary o Body Weight Data {g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DRAFT* 2 3 PPM 50 295* 29.0 50 300* 31.0 50 306 30.2 50 318 33.7 50 334 38.7 50 346 42.7 50 359 48.3 --- FEMALE--34 30 100 PPM PPM 50 288* 27.9 50 293* 28.2 SO 297* 29.3 50 311* 32.8 50 324* 37.2 50 336* 40.2 50 349 44.1 60 263* 23.3 59 266* 23.0 59 270* 23.2 59 277* 24.8 58 286* 26.4 58 294* 28.2 58 301* 31.8 5 300 PPM 0 0 0 0 0 0 0 6 100 PPM 40 266* 21.9 40 269* 21.7 40 272* 22.2 40 279* 23.3 40 291* 24.5 40 299* 26.6 40 307* 30.0 7 300 PPM 0 0 0 0 0 0 0 Covance 6329-212 3M T-6316.1 PAGE: 7 Table 5 Summary of Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS `DRAFT* SEX: --- FEMALE--- GROUP: 1 2 3 4 5 6 7 DOSE: 0 3 30 100 300 100 300 WEEK UNITS: PPM PPM PPM PPM PPM PPM PPM 37 N 55 50 50 58 0 40 0 MEAN 384 366 355* 304* 313* S.D. 58.2 48.9 46.4 34.0 32.2 41 N 55 50 50 58 0 40 0 MEAN 400 380 370 313* 323* S.D. 64.7 52.3 48.2 36.5 34.8 45 N 55 50 50 58 0 40 0 MEAN 423 397 388 320* 332* o S.D. 72.3 59.3 54.0 40.5 38.0 c 49 N 54 50 50 58 0 40 0 C-i MEAN 439 412 396* 329* 339* K* S.D. 76.0 63.2 55.3 43.1 40.6 "J 53 N 54 50 50 58 0 40 0 MEAN 454 425 404* 338* 350* S.D. 82.1 67.9 62.5 48.7 44.3 Covance 6329-212 3M T-6316.1 PAGE: 8 Table 6 Summary of Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DRAFT* WEEK SEX: GROUP: DOSE: UNITS: -------- MALE8 0 PPM 9 1 PPM FEMALE' 89 01 PPM PPM 1N 70 MEAN 204 S.D. 18.8 2N 70 MEAN 260 S.D. 21.9 3N 70 MEAN 309 S.D. 25.3 4N 70 MEAN 351 S.D. 28.4 5N 65 MEAN 381 S.D. 33.1 6N 65 MEAN 409 S.D. 36.9 7N 65 MEAN 433 S.D. 40.2 70 202 19.4 70 260 22.1 70 309 25.4 70 350 28.4 65 383 33.4 65 413 36.4 65 438 40.4 70 152 10.8 70 179 13.9 70 202 16.1 70 224 19.6 65 240 19.9 65 253 22.3 65 263 24.5 70 152 11 70 179 13 70 203 15 70 223 17 65 236 17 65 251 20 65 260 21 Covance 6329-228 3M T-6316.1 PAGE: 1 Table 6 Summary of Body Weight Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DRAFT* WEEK SEX: -------- MALE- GROUP: 8 DOSE: 0 UNITS: PPM 9 1 PPM ------- FEMALE89 01 PPM PPM 8N 65 MEAN 451 S.D. 43.0 65 454 42.9 64 272 25.2 64 267 23.3 9N 65 MEAN 468 S.D. 45.9 65 472 45.3 64 282 27.6 65 274 23.9 10 N 65 MEAN 484 S.D. 49.7 65 490 47.4 64 287 28.4 65 281 25.7 o 11 N 65 65 64 65 MEAN 496 503 287 284 Cm S.D. 54.3 48.9 29.0 26.3 V * 12 N 64 MEAN 510 65 517 64 296 65 288 w S.D. 59.2 51.2 30.2 27.1 13 N 64 MEAN 523 S.D. 58.0 65 529 53.4 64 301 30.7 65 294 27.3 14 N 64 MEAN 531 S.D. 58.7 65 537 54.4 64 306 31.4 65 298 29.8 Covance 6329-228 3M T-6316.1 PAGE: 2 Table 6 Summary of Body Height Data (g) DRAFT* 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS WEEK SEX: -------- MALE- GROUP: 8 9 DOSE: 0 1 UNITS: PPM PPM ------- FEMALE89 01 PPM PPM 15 N 59 MEAN 541 S.D. 60.2 16 N 59 MEAN 553 S.D. 60.8 17 N 59 MEAN 562 S.D. 61.8 21 N 59 MEAN 591 S.D. 68.4 C u 25 N 59 MEAN 617 S.D. 73.3 03 C 29 N 59 MEAN 631 S.D. 76.8 33 N 59 MEAN 652 S.D. 81.0 60 545 56.4 60 555 59.9 60 564 62.7 60 593 70.0 60 616 74.5 60 630 77.5 60 655 84.4 59 307 34.1 59 314 35.0 59 319 35.8 59 332 41.3 59 345 46.7 59 354 48.3 58 371 53.9 60 302 30.8 60 307 32.3 60 309 33.9 60 325 39.3 60 340 46.4 60 348 51.5 60 366 60.0 Covance 6329-228 3M T-6316.1 PAGE: 3 % Table 6 Summary of Body Height Data (g) DRAFT* 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS WEEK SEX: -------- MA]le-------- GROUP: 8 9 DOSE: 0 1 UNITS: PPM PPM ------- FEMJVLE----- 89 01 PPM PPM 37 41 o 6 c< GO 45 49 53 N MEAN S.D. N MEAN S.D. N MEAN S.D. N MEAN S.D. N MEAN S.D. 59 681 85.7 59 697 90.0 59 719 94.9 59 732 98.6 58 751 102.7 60 674 89.2 60 693 93.2 58 709 99.8 58 726 105.2 58 738 110.7 58 389 60.1 58 402 63.8 58 416 68.8 57 431 74.4 56 445 78.1 60 380 60.3 60 392 64.5 60 407 68.9 60 423 72.3 58 436 74.2 Covance 6329-228 3M T-6316.1 PAGE: 4 SEX: GROUP: DOSE: WEEK UNITS: 1 0 PPM 1-2 N MEAN S.D. 2-3 N MEAN S.D. 3-4 N MEAN S.D. 4-5 N MEAN S.D. 5-6 N MEAN S.D. 6-7 N MEAN S.D. 7-8 N MEAN S.D. 70 47 5.5 70 42 5.1 70 37 5.9 65 29 5.9 65 27 4.6 65 23 5.4 65 16 5.1 Table 7 Summary of Body Weight Change Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS `DRAFT* 2 3 PPM 60 47 5.0 60 43 6.1 60 38 5.8 55 28 7.5 55 29 5.5 55 22 5.3 55 13 7.4 3 30 PPM 60 43* 7.2 60 41 7.7 60 35 7.0 55 25* 5.7 55 27 5.2 55 19* 4.6 55 13 4.4 -- MALE---4 100 PPM 70 40* 6.3 70 38* 5.7 70 31* 5.6 65 26* 5.6 65 24* 6.2 65 19* 4.2 65 13 9.9 5 300 PPM 69 31* 6.4 70 30* 5.8 70 20* 5.4 64 16* 5.7 64 16* 5.9 65 10* 5.1 63 5* 14.1 6 100 PPM 40 42* 5.9 40 38* 6.6 40 30* 5.7 40 27 6.5 40 25 6.1 40 11* 16.5 39 20 6.0 7 300 PPM 40 30* 5.2 40 27* 5.8 40 21* 6.2 40 17* 6.3 40 15* 6.0 39 7* 11.3 39 10* 13.0 Covance G329-212 3M T-6316.1 PAGE: 1 Table 7 Summary of Body Height Change Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS `DRAFT* SEX: - --- MALE---- GROUP: 1 2 3 4 5 67 DOSE: 0 3 30 100 300 100 300 WEEK UNITS: PPM PPM PPM PPM PPM PPM PPM 8-9 N MEAN S.D. 60 20 5.9 54 25* 5.0 55 19 8.0 65 16* 6.3 9-10 N MEAN S.D. 60 17 5.1 54 17 4.7 55 14 9.1 65 11* 6.2 10-11 N 60 54 55 65 o MEAN 11 13 13 9 c- S.D. 5.1 5.9 5.2 7.0 11-12 N 60 54 55 65 05 Ci MEAN S.D. 11 6.1 13 5.2 12 4.7 11 7.3 12-13 N MEAN S.D. 60 10 9.4 54 9 12.1 55 9 4.1 65 7 7.5 13-14 N MEAN S.D. 60 10 7.6 54 8 7.6 55 8 5.7 64 6 8.4 14-15 N MEAN S.D. 55 7 5.7 49 8 5.5 50 6 7.8 59 5 5.5 0 0 0 0 0 0 0 39 16 14.2 39 9* 13.0 39 10 7.1 39 12 4.9 38 12 5.8 38 7 3.9 38 6 4.0 0 0 0 0 0 0 0 Covance 6329-212 3M T-6316.1 PAGE: 2 SEX: GROUP: DOSE: UNITS: 1 0 PPM 15-16 N MEAN S.D. 55 10 4.1 16-17 N MEAN S.D. 55 11 5.9 17-21 N MEAN S.D. 55 29 8.6 21-25 N 55 A MEAN 26 c S.D. 7.6 -. 25-29 N 55 MEAN 19 r# S.D. 9.1 29-33 N MEAN S.D. 55 16 10.1 33-37 N MEAN S.D. 55 15 13.8 Table 7 Summary of Body Weight Change Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DRAFT* --- MALE---234 567 3 30 100 300 100 300 PPM PPM PPM PPM PPM PPM 49 11 5.3 49 10 7.1 49 30 8.2 49 26 10.0 49 17 13.3 49 14 30.0 48 18 10.1 50 7* 4.5 50 10 5.5 50 25 8.6 50 21* 8.9 50 15 10.4 50 16 12.1 50 12 7.5 59 9 4.1 59 8* 5.3 59 19* 11.8 59 17* 9.8 59 18 8.2 59 9 11.4 59 10* 9.6 0 0 0 0 0 0 0 38 8 5.1 38 10 5.6 38 21* 10.8 38 16* 11.0 38 20 8.7 38 10 9.9 38 11 8.6 0 0 0 0 0 0 0 Covance 6329-212 3M T-6316.1 PAGE: 3 H Table 7 Summary of Body Height Change Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DRAFT* SEX: - --- MALE---- GROUP: 1 2 3 4 5 6 7 DOSE: 0 3 30 100 300 100 300 WEEK UNITS: PPM PPM PPM PPM PPM PPM PPM 37-41 N MEAN S.D. 55 6 20.8 48 12 15.7 50 7 8.2 59 9 9.3 41-45 N MEAN S.D. 54 25 10.5 48 27 12.3 50 24 9.5 58 19* 10.7 o 45-49 N 54 48 50 58 C MEAN S.D. 19 10.7 18 21.1 20 10.5 15 9.3 Cw 49-53 N 54 48 50 58 MEAN 20 19 18 18 05 S.D. 8.1 18.3 9.1 11.3 07 1-53 N 54 48 50 58 MEAN 500 516 458* 414* S.D. 73.2 84.8 82.1 59.2 0 38 9 25.6 0 37 18* 13.5 0 37 15 20.7 0 36 23 13.0 0 36 432* 80.6 0 0 0 0 0 Covance 6329-212 3M T-6316.1 PAGE: 4 ooaisG SEX: GROUP: DOSE: WEEK UNITS: 1 0 PPM 1-2 N MEAN S.D. 2-3 N MEAN S.D. 3-4 N MEAN S.D. 4-5 N MEAN S.D. 5-6 N MEAN S.D. 6-7 N MEAN S.D. 7-8 N MEAN S.D. 70 23 6.8 70 22 7.7 70 18 6.1 65 14 6.6 65 14 6.2 65 10 6.5 65 7 5.4 Table 7 Summary of Body Weight Change Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYI PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DRAFT* 2 3 PPM 60 22 5.2 60 20 7.3 60 19 5.8 55 12 6.2 55 10* 5.8 55 11 5.4 55 4 6.1 3 30 PPM 60 20 5.7 60 20 6.4 60 17 6.9 55 12 6.0 55 12 5.4 55 8 5.5 55 5 5.6 4 100 PPM 70 17* 5.4 70 19* 5.5 70 15* 4.8 65 10* 4.3 65 9* 4.1 65 4* 5.4 65 7 4.7 5 300 PPM 70 9* 6.3 70 10* 4.6 70 9* 3.9 65 5* 4.8 65 7* 5.0 65 2* 3.9 65 8 4.6 6 100 PPM 39 17* 4.7 40 20 4.3 40 13* 4.7 40 9* 4.2 40 10* 3.8 40 6* 4.2 40 3* 5.7 7 300 PPM 40 12* 4.9 40 11* 4.8 40 9* 3.4 40 4* 4.3 40 6* 3.5 40 4* 4.5 40 3* 3.8 Covance 6329-212 3M T-6316.1 PAGE: 5 WEEK SEX: GROUP: DOSE: UNITS: 1 0 PPM 8-9 N MEAN S.D. 60 9 5.8 9-10 N MEAN S.D. 60 8 S.6 10-11 N 60 MEAN 5 o S.D. 6.4 11-12 N 60 MEAN 7 S.D. 6.7 QS 12-13 N 60 MEAN 4 S.D. 6.2 13-14 N MEAN S.D. 60 2 5.8 14-15 N MEAN S.D. 55 3 5.0 Table 7 Summary of Body Weight Change Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DRAFT* -- FEMALE--- 234 567 3 30 100 300 100 300 PPM PPM PPM PPM PPM PPM 55 10 5.0 55 5 5.9 55 5 6.5 55 3 5.0 55 5 4.8 55 3 5.2 50 3 6.0 55 9 6.0 55 5* 4.9 55 2 5.9 55 5 6.4 55 4 5.1 55 2 4.8 50 2 4.6 65 4* 4.2 65 4* 5.0 65 2 4.9 65 2* 3.8 65 2* 4.6 65 3 4.3 60 1 4.2 0 0 0 0 0 0 0 40 7 5.2 40 4* 5.3 40 2 4.5 40 3 4.9 40 2 4.0 40 3 3.9 40 2 3.8 0 0 0 0 0 0 0 Covance 6329-212 3M T-6316.1 PAGE: 6 SEX: GROUP: DOSE: WEEK UNITS: 1 0 PPM 15-16 N MEAN S.D. 55 6 5.1 16-17 N MEAN S.D. 55 5 5.4 17-21 N 55 MEAN 13 S.D. 7.9 o 21-25 N 55 o MEAN 14 S.D. 10.9 25-29 N 55 QO MEAN 13 QO S.D. 10.9 29-33 N MEAN S.D. 55 13 10.1 33-37 N MEAN S.D. 55 10 12.4 Table 7 Summary of Body Weight Change Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS `DRAFT* -- FEMALE--2 3 4 5 67 3 30 100 300 100 300 PPM PPM PPM PPM PPM PPM 50 5 5.4 50 6 6.0 50 12 8.1 50 16 10.3 50 12 8.8 50 13 11.9 50 7 8.9 50 5 5.1 50 5 4.9 50 14 8.8 50 13 9.0 50 12 7.7 50 13 8.7 50 6 9.7 59 3* 4.1 59 4 4.5 59 7* 4.8 58 9* 6.0 58 8* 6.3 58 7* 7.8 58 3* 9.4 0 0 0 0 0 0 0 40 3* 3.6 40 4 4.3 40 7* 5.4 40 12 5.7 40 8* 6.2 40 8 6.2 40 6 4.6 0 0 0 0 0 0 0 Covance 6329-212 3M T-6316.1 PAGE: 7 Table 7 Summary of Body Height Change Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DRAFT* SEX: - --- FEMALE--- GROUP: 1 2 3 4 5 6 7 DOSE: 0 3 30 100 300 100 300 WEEK UNITS: PPM PPM PPM PPM PPM PPM PPM 37-41 N MEAN S.D. 55 16 12.9 50 14 10.4 50 14 8.6 58 9* 9.8 0 40 10* 5.4 0 41-45 N MEAN S.D. 55 23 12.3 50 17* 12.4 50 18 10.9 58 7* 8.3 0 40 10* 6.0 0 45-49 N MEAN S.D. 54 17 9.2 50 15 10.2 50 9* 16.0 58 8* 8.3 0 40 7* 7.3 0 49-53 N 54 50 50 58 0 40 0 MEAN 15 13 7 9 11 S.D. 11.3 10.2 27.5 8.7 8.0 o o 1-53 N MEAN 54 289 50 260 50 240* 58 172* 0 39 186* 0 S.D. 77.3 62.4 59.2 47.0 42.4 Covance 6329-212 3M T-6316.1 PAGE: 8 Table 8 Summary of Body Weight Change Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DRAFT* SEX: -------- MALE- GROUP: 8 9 DOSE: 0 1 WEEK UNITS: PPM PPM ------- FEMALE- 89 01 PPM PPM 1-2 N MEAN S.D. 2-3 N MEAN S.D. 3-4 N MEAN S.D. 4-5 N MEAN S.D. 5-6 N MEAN S.D. 6-7 N MEAN S.D. 7-8 N MEAN S.D. 70 56 6.2 70 49 7.2 70 42 6.2 65 30 6.3 65 28 5.9 65 24 5.7 65 17 5.3 70 58 5.8 70 49 7.3 70 41 7.2 65 33 7.8 65 30 6.0 65 25 6.3 65 16 6.2 70 27 6.7 70 22 7.7 70 22 7.9 65 15 6.9 65 14 6.8 65 10 7.1 64 10 6.5 70 27 6.1 70 24 8.0 70 20 7.5 65 15 7.0 65 15 6.2 65 9 5.8 64 7 7.2 Covance 6329-228 3M T-6316.1 PAGE: 1 Table 8 Summary of Body Weight Change Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS *DRAFT* WEEK SEX: -------- MALE- GROUP: 8 DOSE: 0 UNITS: PPM ----- ------- FEMALE---- 989 10 1 PPM PPM PPM 8-9 N MEAN S.D. 9-10 N MEAN S.D. 10-11 N MEAN S.D. 11-12 N MEAN S.D. 12-13 N MEAN S.D. 13-14 N MEAN S.D. 14-15 N MEAN S.D. 65 18 5.5 65 16 10.7 65 12 10.7 64 13 7.3 64 12 7.2 64 9 7.7 59 10 8.1 65 18 4.4 65 17 5.2 65 13 5.0 65 14 5.4 65 12 5.7 65 8 4.5 60 10 5.2 63 9 5.6 64 5 6.0 64 0 7.0 64 9 6.1 64 6 6.6 64 5 6.0 59 0 6.5 64 7 5.2 65 7 6.0 65 3* 6.5 65 4* 6.2 65 6 6.1 65 5 6.5 60 2 6.7 Covance 6329-228 3M T-6316.1 PAGE: 2 26TCOO SEX: GROUP: DOSE: WEEK UNITS: 15- N16 MEAN S.D. 16- N17 MEAN S.D. 17-21 N MEAN S.D. 21-25 N MEAN S.D. 25-29 N MEAN S.D. 29-33 N MEAN S.D. 33-37 N MEAN S.D. Table 8 Summary of Body Height Change Data (g) `DRAFT* 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS -MALE- 80 91 PPM PPM -FEMALE- 8 0 91 PPM PPM 59 12 5.8 59 9 5.2 59 29 9.6 59 26 8.7 59 14 9.4 59 21 11.3 59 29 12.4 60 11 6.2 60 8 5.5 60 29 11.0 60 23 8.7 60 14 8.6 60 25 10.6 60 19* 10.2 59 7 5.7 59 4 7.4 59 13 12.0 59 13 10.5 59 9 14.9 58 18 10.5 58 18 10.1 60 5* 5.9 60 2 6.2 60 16 11.2 60 15 12.5 60 8 10.1 60 18 27.9 60 14 27.0 Covance 6329-228 3M T-6316.1 PAGE: 3 003193 Table 8 Summary of Body Height Change Data (g) DRAFT* 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS WEEK SEX: -------- MALE- GROUP: 8 DOSE: 0 UNITS: PPM 9 1 PPM ------- FEMALE89 01 PPM PPM 37-41 N MEAN S.D. 41-45 N MEAN S.D. 45-49 N MEAN S.D. 49-53 N MEAN S.D. 1-53 N MEAN S.D. 59 16 9.9 59 23 12.5 59 13 10.9 58 17 10.5 58 548 93.5 60 19* 9.2 58 18* 10.3 58 17* 10.4 58 12* 11.1 58 536 102.5 58 13 8.5 58 13 11.0 57 14 11.6 56 12 15.7 56 292 72.1 60 12 10. 60 15 10. 60 16 15. 58 13 9. 58 284 70. Covance 6329-228 3M T-6316.1 PAGE: 4 003.194 WEEK SEX: GROUP: DOSE: UNITS: l 0 PPM 1N 68 MEAN 175 S.D. 15.2 2N 69 MEAN 180 S.D. 13.3 3N 65 MEAN 186 S.D. 13.5 4N 65 MEAN 191 S.D. 15.2 5N 64 MEAN 190 S.D. 15.6 6N 62 MEAN 191 S.D. 16.9 7N 58 MEAN 192 S.D. 17.6 Table 9 Summary of Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DRAFT* --- MALE---- 2 34 5 67 3 30 100 300 100 300 PPM PPM PPM PPM PPM PPM 59 171 15.2 59 177 15.2 58 181 16.2 54 185 15.7 52 188 15.5 54 188 15.4 53 190 17.0 58 172 16.6 57 174 17.5 58 176* 17.6 50 177* 14.2 54 180* 16.8 52 179* 15.9 51 180* 14.4 69 170 14.9 68 168* 14.8 67 169* 14.0 63 170* 13.9 65 172* 14.3 64 170* 13.2 64 168* 18.9 68 162* 15.9 68 154* 14.3 67 150* 14.4 63 147* 14.6 60 147* 16.2 62 146* 15.8 59 145* 15.9 39 170 11.3 39 170* 14.9 40 171* 14.1 40 173* 16.6 39 173* 16.0 40 170* 21.1 39 170* 19.5 38 160* 13.3 39 151* 13.2 38 147* 13.0 40 146* 17.5 39 145* 13.9 39 146* 15.9 38 140* 24.0 Covance 6329-212 3M T-6316.1 PAGE: 1 003195 WEEK SEX: GROUP: DOSE: UNITS: 8N MEAN S.D. 9N MEAN S.D. 10 N MEAN S.D. 11 N MEAN S.D. 12 N MEAN S.D. 13 N MEAN S.D. 14 H MEAN S.D. i 0 PPM 55 191 18.4 56 187 16.5 60 186 15.4 58 184 16.8 56 181 17.2 58 188 20.7 52 185 17.9 Table 9 Summary of Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DRAFT* 2 34 5 67 3 30 100 300 100 300 PPM PPM PPM PPM PPM PPM 52 53 65 0 38 0 189 179* 170* 171* 15.2 15.1 14.6 25.4 52 52 64 0 39 0 188 178* 166* 167* 16.4 17.7 13.5 27.8 54 188 18.2 55 178 16.8 65 164* 16.9 0 39 166* 26.1 0 54 52 64 0 39 0 187 177 164* 169* 16.3 15.5 17.9 25.2 53 183 15.8 55 174 16.2 64 162* 19.9 0 38 171* 15.2 0 52 186 15.6 54 178* 16.0 63 165* 18.4 0 37 174* 14.3 0 48 48 59 0 38 0 184 169* 168* 173* 17.6 21.5 15.7 14.6 Covance S329-212 3M T-6316.1 PAGE: 2 % Table 9 Summary of F o o d Consumption Data Ig) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DRAFT* SEX: GROUP: 1 2 3 4 5 67 DOSE: 0 3 30 100 300 100 300 WEEK UNITS: PPM PPM PPM PPM PPM PPM PPM 15 N 54 49 46 57 0 38 0 MEAN 191 189 176* 170* 175* S.D. 19.1 17.9 16.9 11.5 15.5 16 N 51 49 49 59 0 38 0 MEAN 187 188 178* 171* 176* S.D. 13.1 18.4 17.4 13.1 15.3 20 N 54 49 48 57 0 38 0 MEAN 192 194 184 177* 181* S.D. 16.7 17.8 15.9 19.2 17.0 o e 24 N 55 49 50 58 0 38 0 MEAN 190 189 183 177* 180* S.D. 18.2 17.3 16.5 14.2 17.7 Co H- 28 N 54 MEAN 189 49 176* 49 176* 59 176* 0 37 178* 0 W S.D. 17.5 24.2 18.1 14.7 16.2 cn 32 N 55 48 50 57 0 35 0 MEAN 193 190 187 181* 181* S.D. 17.5 28.1 15.8 14.7 18.1 36 N 55 47 49 59 0 38 0 MEAN 195 197 188 185* 187 S.D. 20.7 19.0 16.5 17.0 18.6 Covance 6329-212 3M T-6316.1 PAGE: 3 WEEK SEX: GROUP: DOSE: UNITS: 1 0 PPM 40 44 48 52 <*> 6 La3 b N MEAN S.D. N MEAN S.D. N MEAN S.D. N MEAN S.D. 54 204 26.8 54 195 20.1 54 194 17.6 54 192 17.4 Table 9 Summary of Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DRAFT* --- MALE---- 2 34 567 3 30 100 300 100 300 PPM PPM PPM PPM PPM PPM 46 209 16.4 48 196 17.3 48 196 18.9 48 194 24.0 50 203 19.7 50 188 18.1 50 186 18.0 49 186 17.4 59 195 17.8 57 183* 18.3 58 182* 16.5 58 180* 17.0 0 37 192* 30.6 0 37 184* 18.9 0 37 185 18.0 0 36 183 18.4 .0 0 0 0 Covance 6329-212 3M T-6316.1 PAGE: 4 86T00 Table 9 Summary of Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS `DRAFT* SEX: ------------------------------------- FEMALE GROUP: 1 2 3 4 5 67 DOSE: 0 3 30 100 300 100 300 WEEK UNITS: PPM PPM PPM PPM PPM PPM PPM 1N 63 MEAN 130 S.D. 23.8 2N 61 MEAN 132 S.D. 20.7 3N 57 MEAN 136 S.D. 15.5 4N 55 MEAN 141 S.D. 15.8 5N 55 MEAN 142 S.D. 18.7 6N 55 MEAN 138 S.D. 15.8 7N 53 MEAN 140 S.D. 17.6 56 124 15.2 55 130 15.9 53 135 22.4 42 138 23.0 46 134 15.5 42 138 18.7 44 133 14.9 54 125 16.0 50 127 16.6 49 129 16.7 46 134 15.2 50 136 20.3 45 135 19.6 50 130* 16.9 59 122* 13.2 60 125 16.9 57 124* 14.9 57 127* 15.0 56 122* 12.0 59 121* 11.5 61 117* 10.8 57 112* 14.9 63 105* 15.9 62 99* 14.3 55 96* 12.5 59 97* 16.0 58 96* 12.6 60 95* 11.2 36 121* 16.0 39 122* 17.8 40 122* 14.8 35 121* 13.2 40 122* 13.8 39 117* 13.4 40 116* 16.2 33 115* 14.3 35 111* 16.5 36 101* 12.3 36 100* 13.1 35 98* 10.3 36 98* 14.6 38 98* 16.0 Covance 6329-212 3M T-6316.1 PAGE: 5 Table 9 Summary of Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS `DRAFT* SEX: - --- FEMALE--- GROUP: 1 2 3 4 5 67 DOSE: 0 3 30 100 300 100 300 UNITS: PPM PPM PPM PPM PPM PPM PPM 8N 52 45 44 57 0 33 .0 MEAN 135 140 136 119* 120* S.D. 16.3 23.7 25.0 12.0 16.0 9N 54 47 48 60 0 39 0 MEAN 131 128 127 118* 114* S.D. 14.4 19.2 17.3 23.4 13.7 10 N 51 44 47 60 0 37 0 MEAN 135 130 127 115* 114* S.D. 21.5 19.6 18.4 15.6 10.4 11 N 52 44 49 62 0 37 0 o Cj MEAN S.D. 131 18.9 128 22.2 131 25.6 115* 20.1 116* 13.6 t o 12 N 54 43 51 62 0 38 0 L -L MEAN 127 121 128 113* rr * S.D. 13.6 13.7 16.5 14.4 114* 16.9 tWwA 13 N 56 MEAN 124 47 125 54 125 64 113* 0 39 111* 0 S.D. 15.9 21.8 20.3 20.9 14.0 14 N 52 45 45 57 0 38 0 MEAN 127 126 124 113* 112* S.D. 17.8 22.9 15.6 19.5 11.7 Covance 6329-212 3M T-6316.1 PAGE: 6 Table 9 Summary of Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS `DRAFT* SEX: GROUP: 1 2 3 4 5 6 7 DOSE: 0 3 30 100 300 100 300 WEEK UNITS: PPM PPM PPM PPM PPM PPM PPM 15 N 48 38 42 55 0 39 0 MEAN 127 125 128 114* 114* S.D. 13.4 21.6 16.1 10.8 12.0 16 N 49 42 44 56 0 38 0 MEAN 127 127 127 113* 114* S.D. 13.6 16.1 19.4 10.5 13.4 20 N 48 41 47 53 0 38 0 MEAN 134 132 134 120* 121* S.D. 15.4 19.6 21.0 12.0 13.1 o 24 N 53 47 47 54 0 38 0 0, MEAN 134 140 135 123* 122* C>* S.D. 17.1 24.0 20.1 18.1 12.4 0 28 N 51 46 45 55 0 39 0 ow MEAN 137 136 133 122* S.D. 19.3 19.0 14.6 15.1 125* 13.5 32 N 54 48 48 55 0 36 0 MEAN 140 143 141 130* 129* S.D. 20.7 20.6 21.4 18.0 12.0 36 N 54 47 49 54 0 37 0 MEAN 146 149 142 135* 134* S.D. 23.1 25.0 19.5 27.0 12.8 Covance 6329-212 3M T-6316.1 PAGE: 7 oo Cj o GROUP: DOSE: WEEK UNITS: 40 N MEAN S.D. 44 N MEAN S.D. 48 N MEAN S.D. 52 N MEAN S.D. 1 0 PPM 51 158 21.8 54 154 24.0 53 147 22.6 54 148 24.8 Table 9 Summary of Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DRAFT* 234567 3 30 100 300 100 300 PPM PPM PPM PPM PPM PPM 48 49 56 0 38 0 162 158 148 149 21.1 22.4 24.9 15.2 48 149 20.3 50 150 19.6 56 133* 20.4 0 40 137* 20.3 0 49 50 56 0 39 0 145 142 137* 135* 19.3 19.9 26.5 14.8 46 144 15.7 48 137* 25.2 55 133* 20.8 0 39 130* 15.6 0 Covance 6329-212 3M T-6316.1 PAGE: 8 Tabla 10 Summary of Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS `DRAFT* S E X : -------- MALE GROUP: 8 DOSE: 0 WEEK UNITS: PPM ------ ------- FEMALE----- 91 0 8 91 PPM PPM PPM 1N 70 MEAN 167 S.D. 12.9 2N 69 MEAN 174 S.D. 15.6 3N 69 MEAN 180 S.D. 15.6 4N 63 MEAN 182 S.D. 16.2 5N 63 MEAN 185 S.D. 17.7 6N 62 MEAN 186 S.D. 19.2 7N 65 MEAN 189 S.D. 19.1 69 166 13.9 69 175 17.4 67 180 21.6 64 183 17.7 60 186 19.2 62 187 19.5 64 189 20.1 46 128 13.5 51 129 13.9 63 137 19.3 50 138 13.6 55 136 14.5 55 141 18.8 61 141 17.8 57 126 12.6 55 129 13.5 66 138 20.1 58 138 18.6 53 137 12.3 52 139 13.4 57 137 15.2 Covance 6329-228 3M T-6316.1 PAGE: 1 Table 10 Summary of Food Consumption Data (g) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS `DRAFT* WEEK SEX: -------- MALE- GROUP: 8 DOSE: 0 UNITS: PPM 9 1 PPM FEMALE 89 01 PPM PPM 8N 63 MEAN 188 S.D. 19.6 9N 63 MEAN 188 S.D. 18.9 10 N 63 MEAN 186 S.D. 28.8 11 N 62 MEAN 187 S.D. 19.9 12 N 63 MEAN 192 S.D. 19.4 13 N 64 MEAN 194 S.D. 19.7 14 N 47 MEAN 196 S.D. 19.0 61 188 20.8 64 191 21.0 63 190 17.6 61 187 16.9 64 194 17.6 64 195 18.4 50 189 16.2 54 139 17.4 57 137 18.4 51 138 15.1 52 132 14.9 53 131 16.7 60 13 6 17.6 42 131 13.7 48 137 17 58 135 16 51 136 20 51 127 13 55 134 13 61 140 19 38 138 20 Covance 6329-228 3M T-6316.1 PAGE: 2 corroo Table 10 Summary of Food Consumption Data (g) DRAFT* 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS WEEK SEX: -------- MALE-------- GROUP: 8 9 DOSE: 0 1 UNITS: PPM PPM ------- FEMALE 8 0 PPM 9 1 PPM 15 N 59 MEAN 190 S.D. 19.5 16 N 55 MEAN 192 S.D. 20.3 20 N 58 MEAN 187 S.D. 19.9 24 N 58 MEAN 191 S.D. 21.3 28 N 55 MEAN 194 S.D. 20.0 32 N 59 MEAN 192 S.D. 21.3 36 N 58 MEAN 190 S.D. 18.5 58 193 18.4 60 193 20.6 57 187 19.6 60 190 17.9 56 194 18.0 60 197 20.7 56 192 19.4 56 132 14.8 47 131 18.2 56 136 19.3 57 144 28.2 53 150 21.4 57 149 21.9 57 144 19.1 54 138 22.9 48 134 14.7 52 141 24.4 55 141 20.5 57 151 23.3 59 151 23.2 54 148 15.9 Covance 6329-228 3M T-6316.1 PAGE: 3 Table 10 summary of Food Consumption Data (g) *DRAFT* 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS WEEK SEX: GROUP: DOSE: UNITS: -------- M W JB-------- 89 01 PPM PPM ------- FEMJU ----89 01 PPM PPM 40 N 59 MEAN 189 S.D. 19.9 44 N 58 MEAN 185 S.D. 21.8 o rLoi- 48 N MEAN S.D. 59 182 21.8 C 52 N 58 MEAN 189 C/T S.D. 21.7 60 189 19.6 58 186 21.4 58 187 21.4 57 183 20.7 57 143 17.7 58 143 17.3 56 144 19.9 55 146 27.1 57 144 20.1 58 145 18.6 59 146 27.0 51 148 27.4 Covance 6329-228 3M T-6316.1 PAGE: 4 Table 11 Summary of Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DRAFT* SEX: GROUP: DOSE: UNITS: 2 3 PPM 3 30 PPM -MALE--------45 100 300 PPM PPM 6 100 PPM 7 300 PPM iN MEAN S.D. 59 0.259 0.016 58 2.618 0.170 69 8.724 0.561 68 25.408 1.919 39 8.873 0.572 38 25.193 1.230 2N MEAN S.D. 59 0.231 0.012 57 2.323 0.166 68 7.544 0.467 68 21.735 1.585 39 7.707 0.503 39 21.759 1.462 3N 58 58 67 67 40 38 MEAN 0.210 2.088 6.864 19.533 6.988 19.559 S.D. 0.011 0.123 0.306 1.195 0.339 0.920 c--. 4 N 54 50 63 62 40 40 MEAN 0.197 1.953 6.393 18.112 6.533 18.294 S.D. 0.011 0.108 0.280 0.938 0.338 1.663 & 5 N 52 54 65 59 39 39 MEAN 0.187 1.852 6.067 17.253 6.135 17.415 S.D. 0.009 0.115 0.300 0.987 0.341 0.733 6N MEAN S.D. 54 0.177 0.008 52 1.737 0.102 64 5.698 0.236 62 16.576 0.999 40 5.764 0.435 39 16.815 1.021 7N MEAN S.D. 53 0.172 0.011 51 1.694 0.091 64 5.418 0.409 59 16.058 0.918 39 5.536 0.320 38 15.803 2.209 Covance 6329-212 3M T-6316.1 PAGE: 1 s c*) C-. fg WEEK SEX GROUP DOSE UNITS: PPM 8. N MEAN S.D. 9N MEAN S.D. 10 N MEAN S.D. 11 N MEAN S.D. 12 N MEAN S.D. 13 N MEAN S.D. 14 N MEAN S.D. 52 0.164 0.008 52 0.156 0.010 54 0.152 0.011 54 0.147 0.009 53 0.141 0.009 52 0.143 0.010 48 0.139 0.010 Table 11 Summary of Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DRAFT* 3 30 PPM -MALE45 100 300 PPM PPM .6 100 PPM 7 300 PPM 53 1.629 0.099 52 1.564 0.104 55 1.517 0.105 52 1.472 0.086 55 1.421 0.101 54 1.426 0.097 48 1.357 0.153 65 5.329 0.251 64 5.031 0.280 65 4.871 0.355 64 4.772 0.324 64 4.616 0.364 63 4.616 0.346 59 4.655 0.348 0 0 0 0 0 0 0 38 5.325 0.496 39 5.043 0.592 39 4.940 0.477 39 4.926 0.349 38 4.800 0.279 37 4.808 0.263 38 4.709 0.241 0 0 0 0 0 0 0 Covance 6329-212 3M T-6316.1 PAGE: 2 Table 11 Summary of Test Material Consumption Data (mg/kg/clay) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS `DRAFT* SEX: GROUP: DOSE: UNITS: 2 3 PPM 3 30 PPM -MALE--------45 100 300 PPM PPM 6 100 PPM 7 300 PPM 15 N MEAN S.D. 16 N MEAN S.D. 20 N MEAN S.D. 24 N MEAN S.D. 28 N MEAN S.D. 32 N MEAN S.D. 36 N MEAN S.D. 49 0.140 0.009 49 0.137 0.009 49 0.134 0.008 49 0.126 0.008 49 0.114 0.018 48 0.120 0.016 47 0.120 0.008 46 1.397 0.115 49 1.384 0.105 48 1.367 0.087 50 1.309 0.090 49 1.231 0.116 50 1.276 0.093 49 1.261 0.102 57 4.641 0.222 59 4.608 0.248 57 4.545 0.340 58 4.447 0.291 59 4.300 0.275 57 4.298 0.303 59 4.355 0.428 0 0 0 0 0 0 0 38 4.694 0.241 38 4.661 0.227 38 4.586 0.264 38 4.438 0.245 37 4.249 0.246 35 4.246 0.250 38 4.305 0.255 0 0 0 0 0 0 0 Covance 6329-212 3M T-6316.1 PAGE: 3 Table 11 Summary of Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DRAFT* SEX: GROUP: DOSE: WEEK UNITS: 2 3 PPM 3 30 PPM -------- MALE45 100 300 PPM PPM 6 100 PPM 7 300 PPM 40 N MEAN S.D. 46 0.126 0.009 50 1.340 0.110 59 4.532 0.407 44 N MEAN S.D. 48 0.115 0.007 50 1.203 0.094 57 4.103 0.346 C 48 N 48 50 58 MEAN 0.111 1.155 3.994 S.D. 0.010 0.095 0.321 fw o 52 N 48 49 58 C2 MEAN 0.108 1.126 3.847 S.D. 0.012 0.084 0.332 0 0 0 0 37 4.358 0.631 37 4.069 0.229 37 3.996 0.268 36 3.841 0.222 0 0 0 0 Covance 6329-212 3M T-6316.1 PAGE: 4 H Table 11 Summary of Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DRAFT* GROUP: DOSE: WEEK UNITS: 2 3 PPM 3 30 PPM 4 100 PPM 5 300 PPM 6 100 PPM 7 300 PPM 1N MEAN S.D. 56 0.307 0.037 54 3.070 0.386 59 10.166 1.080 57 28.712 3.562 36 10.135 1.157 33 29.270 3.365 2N MEAN S.D. 55 0.286 0.033 50 2.791 0.319 60 9.372 1.179 63 25.653 4.087 39 9.093 1.058 35 26.381 4.036 3N 53 49 57 62 40 36 o MEAN 0.271 2.595 8.509 22.650 8.376 22.505 o S.D. 0.051 0.317 0.873 3.249 0.800 2.640 4N 42 46 57 55 35 36 MEAN 0.256 2.540 8.314 21.385 7.946 21.593 S.D. 0.038 0.238 0.912 2.132 0.574 2.667 o 5N 46 50 56 59 40 35 MEAN 0.239 2.442 7.581 20.872 7.644 20.702 S.D. 0.023 0.271 0.610 2.782 0.593 1.771 6N MEAN S.D. 42 0.234 0.027 45 2.312 0.271 59 7.316 0.624 58 20.229 2.277 39 7.115 0.638 36 20.300 3.150 7N MEAN S.D. 44 0.219 0.018 50 2.183 0.225 61 6.942 0.555 60 19.568 1.815 40 6.839 0.727 38 20.004 3.757 Covance 6329-212 3M T-6316.1 PAGE: 5 TTJ-POO Table 11 Summary of Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS *DRAFT* SEX: ------------------------------- FEMALE----------------------------- GROUP: 2 3 4 5 6 7 DOSE: 3 30 100 300 100 300 WEEK UNITS: PPM PPM PPM PPM PPM PPM 8N MEAN S.D. 9N MEAN S.D. 10 N MEAN S.D. 11 N MEAN S.D. 12 N MEAN S.D. 13 N MEAN S.D. 14 N MEAN S.D. 45 0.225 0.040 47 0.201 0.029 44 0.199 0.027 44 0.193 0.035 43 0.181 0.023 47 0.184 0.031 45 0.182 0.031 44 2.237 0.390 48 2.007 0.203 47 1.993 0.228 49 2.016 0.342 51 1.952 0.246 54 1.879 0.275 45 1.846 0.155 57 6.907 0.675 60 6.656 1.398 60 6.413 0.760 62 6.456 1.352 62 6.213 0.720 64 6.200 1.400 57 6.174 1.263 0 0 0 0 0 0 0 33 7.017 0.722 39 6.458 0.446 37 6.488 0.543 37 6.508 0.898 38 6.312 0.861 39 6.045 0.705 38 6.058 0.429 0 0 0 0 0 0 0 Covance 6329-212 3M T-6316.1 PAGE: 6 Table 11 Summary of Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS `DRAFT* GROUP: DOSE: WEEK UNITS: 2 3 PPM 3 30 PPM 4 100 PPM 5 300 PPM 15 N MEAN S.D. 38 0.179 0.028 42 1.873 0.178 55 6.130 0.515 0 16 N MEAN S.D. 42 0.177 0.018 44 1.841 0.217 56 6.010 0.423 0 O 20 N MEAN 41 0.178 47 1.844 53 6.163 O* S.D. 0.027 0.184 0.484 24 N 47 47 54 fw MEAN 0.183 1.782 6.214 k* S.D. 0.036 0.192 1.082 10 28 N 46 45 55 MEAN 0.170 1.696 5.916 S.D. 0.025 0.120 0.575 0 0 0 32 N MEAN S.D. 48 0.172 0.028 48 1.739 0.207 55 6.162 0.808 0 36 N MEAN S.D. 47 0.175 0.031 49 1.732 0.205 54 6.349 1.458 0 6 100 PPM 39 6.128 0.383 38 6.018 0.475 38 6.206 0.436 38 6.030 0.348 39 5.977 0.413 36 6.080 0.475 37 6.125 0.448 7 300 PPM 0 0 0 0 0 0 0 Covance 6329-212 3M T-6316.1 PAGE: 7 Table 11 Summary of Test Material Consumption Data (mg/kg/day) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS `DRAFT* GROUP: DOSE: WEEK UNITS: 2 3 PPM 3 30 PPM 4 100 PPM 5 300 PPM 40 N MEAN S.D. 44 N MEAN S.D. e 48 N o MEAN S.D. f w 52 N MEAN S.D. CO 48 0.184 0.027 48 0.163 0.020 49 0.153 0.018 46 0.147 0.018 49 1.853 0.258 50 1.677 0.195 50 1.550 0.215 48 1.454 0.250 56 6.788 1.158 56 5.946 0.888 56 5.976 1.169 55 5.686 0.899 0 0 0 0 6 100 PPM 38 6.631 0.448 40 5.912 0.783 39 5.715 0.425 39 5.355 0.552 7 300 PPM 0 0 0 0 Covance 6329-212 3M T-6316.1 PAGE: 8 Table 12 Summary of Test Material Consumption Data (mg/kg/day) `DRAFT* 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS WEEK SEX: GROUP: DOSE: UNITS: --MALE----- --FEMALE99 11 PPM PPM 1N 69 57 MEAN 0.103 0.108 S.D. 0.007 0.010 2N MEAN S.D. 69 0.088 0.007 55 0.096 0.009 3N 67 66 o MEAN 0.078 0.092 o S.D. 0.008 0.013 C,5 4 N 64 58 ?v MEAN 0.071 0.086 H* S.D. 0.004 0.011 H-* 5 N 60 53 MEAN 0.066 0.080 S.D. 0.004 0.005 6N MEAN S.D. 62 0.063 0.003 52 0.077 0.007 7N 64 56 MEAN 0.061 0.075 S.D. 0.005 0.008 Covance 6329-228 3M T-6316.1 PAGE: 1 Table 12 Summary of Test Material Consumption Data (mg/kg/day) DRAFT* 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS WEEK SEX: ---MALE--- GROUP: 9 DOSE: 1 UNITS: PPM --FEMALE9 1 PPM 8N MEAN S.D. 61 0.058 0.005 47 0.072 0.010 9N MEAN S.D. 64 0.057 0.004 58 0.070 0.009 o 10 N 63 51 o MEAN 0.055 0.069 S.D. 0.004 0.010 Cui 11 N 61 51 H1 MEAN S.D. 0.053 0.003 0.063 0.007 C/l 12 N 64 55 MEAN 0.053 0.066 S.D. 0.004 0.006 13 N MEAN S.D. 64 0.052 0.003 61 0.067 0.009 14 N MEAN S.D. 50 0.051 0.003 38 0.065 0.009 Covance 6329-228 3M T-6316.1 PAGE: 2 Table 12 Summary of Test Material Consumption Data (mg/kg/day) DRAFT* 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS SEX: --- MALE--- -- FEMALE- GROUP: 9 9 DOSE: 1 1 WEEK UNITS : PPM PPM 15 N MEAN S.D. 58 0.050 0.003 54 0.064 0.012 16 N MEAN S.D. 60 0.049 0.003 48 0.062 0.006 20 N 57 52 e MEAN 0.045 0.062 o S.D. 0.003 0.012 (Li 24 N 60 55 MEAN 0.045 0.060 S.D. 0.004 0.009 O 28 N 56 57 MEAN 0.044 0.062 S.D. 0.004 0.008 32 N MEAN S.D. 60 0.043 0.003 59 0.060 0.010 36 N MEAN S.D. 56 0.041 0.003 54 0.056 0.007 Covance 6329-228 3M T-6316.1 PAGE: 3 Table 12 Summary of Test Material Consumption Data (mg/kg/day) DRAFT* 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS WEEK SEX: --- MALE--- GROUP: 9 DOSE: 1 UNITS: PPM -- FEMALE9 1 PPM 40 N MEAN S.D. 44 N MEAN S.D. o 48 N c MEAN S.D. tLsX) 52 N MEAN r " S.D. 60 0.039 0.003 58 0.038 0.003 58 0.037 0.003 57 0.036 0.003 57 0.053 0.007 58 0.052 0.006 59 0.050 0.009 51 0.049 0.008 Covance 6329-228 3M T-6316.1 PAGE: 4 Table 13 Summary of Clinical Hematology Data Males Week 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm RBC HGB HCT MCV MCH MCHC PLT X10(/fih G/DL FL PG % X10V/iL Covance 6329-212 3M T-6316.1 0 MEAN S.D. N 8.27 .267 5 15.3 .44 5 44.7 1.43 5 54.1 1.10 5 18.5 .40 5 34.1 .61 5 1269 111 5 300 MEAN 6.61 * 12.7 * 37.1 * 56.3 19.2 34.1 1092 <> S.D. .609 .68 2.14 2.65 .82 .72 112 N 5555555 t:. 03 DOSE ppm WBC XlOV/iL Table 13 Summary of Clinical Hematology Data Males Week 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS N-SEG LYMPH X10*/fiL X10*//iL MONO EOSIN BASO N-SEG% X10*/lj XlO'/^iL XlOV/iL LYMPH% MONO% EOSIN% Covane 329-212 3M T-6316.1 BASO% 0 MEAN 8.5 1.2 6.9 .4 .1 S.D. 3.27 .41 3.52 .29 .04 N 55555 0 15 78 6 1 0 00 6.1 8.8 4.0 .5 555 55 5 300 MEAN 6.5 1.0 5.2 .3 .0 * 0 17 78 4 1 0 S.D. 1.98 .29 2.10 .19 .05 00 11.7 12.1 2.9 .4 N 55555555555 O Cm e Co ot o Table 13 Sumciary of Clinical Hematology Data Females Week 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm RBC HGB HCT MCV HCH MCHC PLT X10`/m L G/DL % FL PG % XIOV^L 0 MEAN S.D. N 300 MEAN S.D. N 7.83 .321 5 6.83 * .388 5 14.7 .40 5 13.0 * .81 5 43.6 1.62 5 37.9 * 2.43 5 55.6 1.13 5 55.5 1.31 5 18.8 .56 5 19.0 .46 5 33.8 .48 5 34.2 .29 5 1114 88 5 1110 113 5 Covance 6329-212 3M T-6316.1 DOSE ppm WBC XIOVm L Table 13 Summary of Clinical Hematology Data Females Week 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS N-SEG LYMPH XIOV^L XIOV/JL MONO EOSIN BASO N-SEG* XlO'/nL XlOVjiL XIOV^L LYMPH% MONO% EOSIN% Covance 6329-212 3M T-6316.1 BASO% MEAN 7.7 .7 6.6 .3 .1 .0 10 84 4 2 0 S.D. 2.85 .31 2.95 .11 .09 .00 6.5 9.1 2.2 1.7 N 55555555555 MEAN 5.8 .6 5.0 .2 .0 * .0 10 86 3 1 0 S.D. .78 .30 .93 .10 .00 .00 6.4 5.7 1.1 .4 N 55555555555 O O c. Table 14 Sunmary of Clinical Hematology Data Hales Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) [-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm RBC HGB HCT MCV MCH MCHC PLT X10`/^L G/DL % FL PG % X10'/<iL Covance 6329-212 3M T-6316.1 0 MEAN 8.54 15.3 45.7 53.6 17.9 33.5 1240 S.D. .403 .48 1.34 1.46 .55 .53 156.0 N 10 10 10 10 10 10 10 3 MEAN S.D. N 8.72 .268 10 15.6 .45 10 46.6 1.17 10 53.5 1.21 10 17.9 .54 10 33.5 .47 10 1140 67.8 10 rro o 30 MEAN S.D. N 8.33 .455 10 15.0 .71 10 44.6 1.87 10 53.6 1.45 10 18.0 .38 10 33.7 .37 10 1143 158.3 10 13 100 IZ MEAN S.D. 8.18 .347 15.0 .43 44.4 1.57 54.4 1.23 18.4 .51 33.8 .42 1141 80.6 N 10 10 10 10 10 10 10 DOSE ppm WBC XlOV/iL Table 14 summary of Clinical Hematology Data Hales Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS N-SEG LYMPH XIOVliL XlOV/iL HONO EOSIN BASO X10Vi<L XIOV^L XlOV^L N-SEG% LYMPH% MONO% EOSIN% Covance 6329-212 3M T-6316.1 BASO% 0 MEAN 7.4 1.2 5.3 .7 .1 .0 17 71 10 2 0 S.D. 1.31 .38 1.27 .19 .04 .00 5.2 7.1 2.8 .6 .0 N 10 10 10 10 10 10 10 10 10 10 10 3 MEAN 10.4 1.5 7.9 .8 .2 .0 15 75 7 2 0 S.D. 3.31 .54 2.79 .29 .11 .00 4.6 6.6 2.3 .7 .0 N 10 10 10 10 10 10 10 10 10 10 10 o 30 o MEAN 9.2 1.3 7.0 .7 .2 .0 15 75 8 2 0 c, S.D. 1.85 .41 1.59 .28 .07 .00 4.6 4.7 2.4 .6 .0 N 10 10 10 10 10 10 10 10 10 .10 10 w 100 rw s MEAN 8.8 1.3 6.7 .7 .1 .0 15 76 8 1 0 S.D. 2.64 .39 2.23 .24 .05 .00 2.6 3.3 2.5 .5 .0 N 10 10 10 10 10 10 10 10 10 10 10 Table 14 Summary of Clinical Hematology Data Females Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm RBC HGB HCT MCV MCH MCHC PLT X10V/iL G/DL % FL PG % XlOV/iL Covance 6329-212 3M T-6316.1 0 MEAN S.D. N 8.15 .469 10 15.5 .65 10 45.9 2.09 10 56.4 1.17 10 19.0 .61 10 33.7 .46 10 1040 113 10 3 MEAN S.D. N 8.12 .411 10 15.3 .64 10 45.3 2.16 10 55.8 1.97 10 18.8 .67 10 33.7 .38 10 1000 222 10 o 30 MEAN 8.01 15.2 45.5 56.8 19.1 33.6 1054 S.D. .419 .55 1.63 1.72 .80 .45 124 N 10 10 10 10 10 10 10 100 MEAN S.D. N 7.79 .258 9 14.9 .23 9 43.7 .82 9 56.2 1.46 9 19.2 .61 9 34.1 .54 9 1023 56 9 DOSE ppm WBC XIOV/iL Table 14 Summary of Clinical Hematology Data Females Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESOLFONAMIDO ETHANOL IN RATS N-SEG LYMPH XlOV/iL X10V/<L MONO EOSIN BASO XlOV/iL XlOVfiL XIOVm L N-SEG% LYMPH% MONO% EOSIN% Covance 6329-212 3M T-6316.1 BASO% 0 MEAN 6.4 .8 5.1 .4 .1 .0 13 79 62 0 S.D. 1.63 .22 1.44 .13 .04 .00 3.6 . 4.2 1.4 .6 .0 N 10 10 10 10 10 10 10 10 10 10 10 3 MEAN 6.9 .8 5.5 .4 .1 .0 11 81 6 2 0 S.D. 1.77 .43 1.48 .12 .03 .00 4.5 4.8 2.0 .8 .0 N 10 10 10 10 10 10 10 10 10 10 10 o 30 o MEAN 7.5 .8 6.3 .4 .1 .0 12 80 5 2 0 C- S.D. 5.43 .22 5.06 .19 .09 .00 5.5 5.9 1.8 .5 .0 W N 10 10 10 10 10 10 10 10 10 10 10 4 100 07 MEAN 6.2 .6 5.1 .4 .1 .0 11 80 6 2 0 S.D. 1.80 .26 1.68 .12 .06 .00 4.8 6.6 3.0 .7 .0 N 99999999999 oo C.1 A? to & Table 15 Summary of C-lfiinniiccaall HHeemmaattoollooggyy EData kr*<~-- Males Week 14 DOSB PPm 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS N-ETHYL X 1 0R`B/C/<D HG/GDBL HCT HCT HCV PL m c h MEAN S.D. N mean S.D. N 8-32 838 10 8.38 <U1 10 15.2 1.18 10 IS.o 64 10 44.3 1.23 10 44.1 1.62 10 S3. 4 1.55 10 S2.5 1.35 10 PG 18.3 .80 10 17.9 .53 10 Clic % . DDT X10Vm , 34.2 45 10 34.0 31 10 1094 94.e 10 1094 90.4 10 COVan3* *'129-228 3M l''63l|218 DOSE ppm Table 15 Summary of Clinical Hematology Data Males Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS WBC XIOVjiL N-SEG LYMPH XlO'/^L XIOV/iL MONO EOSIN BASO X10V/iL XIOV^L X10V/iL N-SEG% LYMPH% MONO% EOSIN% Covance 329-228 3M T-6316.1 BASO% MEAN 10.4 1.3 7.9 .9 .3 .0 13 76 9 3 0 S.D. 2.18 .28 2.04 .23 .12 .00 2.6 . 5.2 2.9 1.3 .0 N 10 10 10 10 10 10 10 10 10 10 10 MEAN 10.2 1.3 7.5 1.1 .3 .0 13 74 11 2 0 S.D. 2.12 .39 1.72 .50 .18 .03 4.1 4.4 3.9 1.3 .0 N 10 10 10 10 10 10 10 10 10 10 10 O C-; Table IS Summary of Clinical Hematology Data Females Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS RBC HGB HCT MCV MCH MCHC PLT X10`/ML G/DL % FL PG % XIOV^L Covance 6329-228 3M T-6316.1 MEAN S.D. N 7.82 .358 10 14.7 .73 10 43.1 2.10 10 55.1 1.05 10 18.8 .53 10 34.2 .45 10 1064 88 10 MEAN S.D. N 8.01 .350 10 15.2 .39 10 44.2 1.44 10 55.3 1.32 10 19.0 .53 10 34.4 .52 10 1112 83 10 o c> ra 13 GO Table 15 Summary of Clinical Hematology Data Females Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS WBC XlO'/^iL N-SEG LYMPH X10*/i<L XIOV^I* MONO EOSIN BASO X10V//L XIOV^L X10V/iL N-SEG% LYMPH% MONO% EOSIN% Covance 6329-228 3M T-6316.1 BASO% MEAN 7.3 .8 5.8 .5 .2 .0 12 78 7 3 0 S.D. 2.61 .38 2.24 .16 .09 .00 4.3 4.7 1.7 .9 0 N 10 10 10 10 10 10 10 10 10 10 10 MEAN 6.5 .9 5.0 .4 .2 .0 15 75 7 3 0 S.D. 1.23 .20 1.37 .17 .06 .00 5.0 8.7 3.7 1.0 0 N 10 10 10 10 10 10 10 10 10 10 10 cO e- ?w M (2 Table 16 Summary of Clinical Hematology Data Males Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm RBC HGB HCT MCV MCH MCHC PLT X10`/^L G/DL % FL PG % X10V/iL Covance 6329-212 3M T6316.1 0 MEAN S.D. N 8.33 .445 10 14.8 .47 10 44.2 1.41 10 53.1 1.63 10 17.8 .66 10 33.4 .40 10 1270 107.3 10 3 MEAN 8.19 14.6 44.0 53.8 17.9 33.3 1096 * S.D. .879 1.46 4.15 1.83 .66 .45 100.5 N 10 10 10 10 10 10 10 30 MEAN 8.27 14.9 44.0 53.2 18.0 33.8 1190 S.D. .374 .51 1.12 1.98 .78 .44 131.4 N 10 10 10 10 10 10 10 c - 100 MEAN 8.22 14.5 42.9 52.2 17.6 33.7 1206 u S.D. .398 .59 2.12 2.24 .77 .44 55.6 N 10 10 10 10 10 10 10 O c f. v IA DOSE ppm WBC XlOV/iL Table 16 Summary of Clinical Hematology Data Males Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE <98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS N-SEG LYMPH XIO'/aaL XlOV/iL MONO EOSIN BASO XIOVaaL XIOVaaL XIOVaaL N-SEG% LYMPH% MONO% EOSIN% Covance 6329-212 3M T6316.1 BASO% 0 MEAN 7.9 1.4 5.5 .8 .2 .0 19 69 10 2 0 S.D. 1.77 .34 1.52 .19 .06 .00 4.2 4.7 1.8 .4 .0 N 10 10 10 10 10 10 10 10 10 10 10 3 MEAN 7.9 1.4 5.6 .7 .2 .0 17 71 92 0 S.D. 1.62 .65 1.14 .23 .08 .00 4.5 5.3 4.1 .7 .3 N 10 10 10 10 10 10 10 10 10 10 10 30 MEAN 8.8 2.0 5.8 .8 .2 .0 22 68 92 0 S.D. 1.91 .98 1.22 .27 .07 .00 8.3 8.7 2.2 .8 ,0 N 10 10 10 10 10 10 10 10 10 10 io' 100 MEAN 8.9 2.4 5.6 .8 .1 .0 24 65 92 0 S.D. 3.06 2.34 1.27 .28 .04 .00 11.6 11.7 2.4 .7 .3 N 10 10 10 10 10 10 10 10 10 10 10 Table 16 Summary of Clinical Hematology Data Females Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm RBC HGB HCT MCV MCH MCHC PLT X10`/m L G/DL % FL PG % XIOV^L Covance 6329-212 3M T6316.1 0 MEAN S.D. N 7.93 .316 10 15.3 .29 10 45.1 1.04 10 56.9 1.76 10 19.3 .76 10 33.9 .38 10 1076 96.6 10 3 MEAN 7.54 * 14.7 * 43.2 * 57.4 19.5 34.0 987 S.D. .243 .50 1.23 1.88 .82 .49 176.1 o N 10 10 10 10 10 10 10 o 30 MEAN 7.78 14.9 43.9 56.4 19.1 33.9 968 S.D. .396 .46 1.38 1.99 .80 .49 94.2 N 10 10 10 10 10 10 10 u t4 100 MEAN 7.25 * 14.1 * 41.0 * 56.5 19.4 34.4 996 S.D. .268 .61 1.82 1.74 .51 .56 103.9 N 10 10 10 10 10 10 10 DOSE ppm WBC XlO'/AiL Table 16 Summary of Clinical Hematology Data Females Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS N-SEG LYMPH X105//rL X10V/JL MONO EOSIN BASO XIOVa/L x i o V m l XIOV^L N-SEG% LYMPH% MONO% EOSIN% Covance 6329-212 3M T6316.1 BASO% 0 MEAN 5.1 .7 3.7 .6 .1 .0 14 71 12 2 0 S.D. 1.29 .17 1.39 .21 .00 .03 5.2 10.3 5.5 6 .7 N 10 10 10 10 10 10 10 10 10 10 10 3 MEAN 5.2 .7 3.9 .5 .1 .0 14 74 10 2 0 S.D. 1.85 .24 1.55 .17 .03 .00 2.8 4.0 3.2 .9 .3 N 10 10 10 10 10 10 10 10 10 10 10 30 MEAN 4.8 .8 3.3 .5 .1 .0 18 69 11 2 0 O S.D. 1.20 .22 .98 .21 .03 .00 4.6 7.5 4.1 .7 .4 N 10 10 10 10 10 10 10 10 10 10 10 100 MEAN 4.9 .8 3.5 .4 .1 .0 17 72 8 2 0 to S.D. .69 .28 .73 .07 .03 .00 5.7 6.7 2.2 .5 .4 N 10 10 10 10 10 10 10 10 10 10 10 to Male -WEEK DIETARY CARCINOGENICITY _f Do s e Ppm mean S.D. N mean S.D. N RBC Xl 0`/uL 8.22 430 10 8.28 487 10 P. ER-F,,LnUcOiRNOOOGCETNAINCEISTtY' STUDY WITH NARROW RANGE PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS HHGGBB Gg //dml HCT t MCV FL MC PG MCHC 15.2 .67 10 *4.8 .78 10 <4.6 1.98 10 <3. g 2-19 10 54.3 1.63 10 53.1 1.28 10 18.5 62 10 17.9 54 10 34.0 36 10 33.8 * .39 10 PjLT* xi o y ^ 1083 109.2 10 1112 83.2 10 C V ance 6 3 2 9 -2 2 8 3M 3 1 f 2i8 DOSE ppm Table 17 Summary of Clinical Hematology Data Males Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS WBC N-SEG LYMPH XIO'/fiL XIOV/^L X10V#<L MONO EOSIN BASO XIOV^L XIOV/^L XIOV^L N-SEG% LYMPH% MONO% EOSIN% Covance 6329-228 3M T6316.1 BASO% 0 MEAN 8.6 1.7 5.7 1.0 .2 .0 19 66 11 3 0 S.D. 1.85 .81 1.12 .34 .13 .04 5.4 8.0 4.0 1.6 N 10 10 10 10 10 10 10 10 10 10 10 1 MEAN 8.9 1.5 5.9 1.2 .2 .1 17 66 14 2 1 S.D. 1.34 .28 1.17 .67 .08 .13 4.0 7.6 6.2 1.0 N 10 10 10 10 10 10 10 10 10 10 10 C o to to to m Table 17 Summary of Clinical Hematology Data Females Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm RBC HGB HCT MCV MCH MCHC PLT XIOViiL G/DL % FL PG f XIOV^L 0 MEAN S.D. N 1 MEAN S.D. 7.31 .267 10 7.63 .451 13.9 .75 10 14.7 * .54 40.6 1.79 10 42.5 * 1.93 55.6 1.46 10 55.8 1.45 19.1 .66 10 19.3 .58 34.3 .50 10 34.6 .41 1038 101 10 1098 161 Covance 6329-228 3M T6316.1 DOSE ppm WBC XIOVpL Table IT Summary of Clinical Hematology Data Females Meek 21 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS N-SEG LYMPH X10V/iL X10Va<L MONO EOSIN BASO XIOVaL XlOVnL X10V/aL N-SEG% LYMPH MONO EOSIN Covance 6329-228 3M T6316.1 BASO 0 MEAN 6.4 1.0 4.6 .6 .2 .0 15 73 92 0 S.D. 2.32 .72 1.46 .28 .15 .00 5.7 7.7 2.3 1.2 N 10 10 10 10 10 10 10 . 10 10 10 10 1 MEAN 5.8 .9 4.2 .5 .1 .0 16 72 92 0 S.D. 1.90 .30 1.63 .21 .04 .00 5.8 9.9 3.6 .8 N 10 10 10 10 10 10 10 10 10 10 10 oc CO >1 Table 18 Summary of Clinical Hematology Data Males Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) I-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm RBC HGB HCT MCV MCH MCHC PLT X10`/a<L G/DL % FL PG % XIO'/^L Covance 6329-212 3M T6316.1 0 MEAN S.D. N 8.01 1.004 10 14.5 1.52 10 43.3 4.50 10 54.2 2.32 10 18.2 .78 10 33.4 .30 10 1238 229.3 10 3 MEAN S.D. N 8.42 .498 10 15.2 .76 10 45.3 2.79 10 53.8 1.88 10 18.1 .52 10 33.6 .61 10 1210 176.3 10 30 MEAN S.D. N 8.39 .585 10 14.8 1.12 10 43.8 3.17 10 52.2 1.82 10 ' 17.6 .80 10 33.8 .56 10 1199 97.5 10 O 100 MEAN 8.45 14.7 43.3 51.3 * 17.4 * 33.9 1129 o S.D. .454 .68 1.73 1.06 .45 .55 184.1 Ct,--.1 N 10 10 10 10 10 10 10 Cj DOSE ppm WBC XIOVwL Table 18 Summary of Clinical Hematology Data Males Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS N-SEG LYMPH XlO'/iiL XIO'/^L MONO EOSIN BASO x i o '/m l XIOViiL x i o 5/m l N-SEG% LYMPH% MONO% EOSIN% Covance 6329-212 3M T6316.1 BASO% 0 MEAN 8.5 2.4 4.9 1.0 .2 .0 29 58 12 2 0 S.D. 2.14 .88 1.26 .35 .08 .00 5.1 5.4 3.4 .7 .3 N 10 10 10 10 10 10 10 10 10 10 10 3 MEAN 7.9 1.9 4.9 .9 .1 .0 24 62 11 2 0 S.D. 1.11 .73 .70 .28 .05 .03 6.9 6.6 3.2 .7 3 N 10 10 10 10 10 10 10 10 10 10 10 30 MEAN 8.8 2.5 5.5 .7 .2 .0 28 62 82 0 S.D. .90 .79 1.01 .43 .07 .00 8.3 8.8 4.6 .7 .0 N 10 10 10 10 10 10 10 10 10 10 10 100 MEAN 7.5 1.8 4.7 .8 .2 .0 25 62 11 2 0 c- S.D. 2.02 .53 1.74 .19 .05 .00 7.1 9.5 3.6 .6 .3 N 10 10 10 10 10 10 10 10 10 10 10 CD CO Table 18 Summary of Clinical Hematology Data Females Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm RBC HGB HCT MCV MCH MCHC PLT X10V//L G/DL % FL PG % XIOVwL Covance 6329-212 3M T6316.1 0 MEAN S.D. N 7.23 1.388 10 14.2 1.95 10 41.5 6.15 10 58.2 4.19 10 20.0 1.81 10 34.4 .64 10 998 125 10 3 MEAN S.D. N 7.42 .589 10 14.3 1.03 10 42.4 2.99 10 57.2 1.78 10 19.3 .85 10 33.7 * .56 10 935 125 10 30 MEAN 7.44 14.6 42.8 57.6 19.6 34.0 918 S.D. .979 1.86 5.57 2.13 .83 .51 133 N 10 10 10 10 10 10 10 c 100 Co MEAN 6.92 13.6 39.4 57.1 19.7 34.6 907 S.D. 1.008 1.62 4.74 2.24 .79 .43 80 N 10 10 10 10 10 10 10 o WBC XIOViiL Table 18 Summary of Clinical Hematology Data Females Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS N-SEG LYMPH XIOV/iL XIOV/jL MONO EOSIN BASO XIOV^L XIOV^L XlOV/iL N-SEG% LYMPH% MONO% EOSIN% Covance 6329-212 3M T6316.1 BASO% 0 MEAN 4.5 1.1 2.7 .6 .1 .0 25 60 13 2 0 S.D. .59 .39 .40 .08 .03 .00 6.8 6.8 1.2 .5 .4 N 10 10 10 10 10 10 10 10 10 10 10 3 MEAN 4.8 1.5 2.6 .6 .1 .0 26 59 12 2 0 S.D. 1.95 1.60 .74 .34 .03 .03 13.8 14.8 1.9 .9 .3 N 10 10 10 10 10 10 10 10 10 10 10 30 O MEAN 5.7 1.2 3.8 .5 .1 .0 22 66 9 3 0 Cj S.D. 1.60 .49 1.43 .20 .07 .00 7.4 8.4 3.8 1.4 .0 & N 10 10 10 10 10 10 10 10 10 10 10 fvi' K 100 MEAN 5.8 * 1.6 3.4 .7 .2 .0 28 58 11 3 0 S.D. .74 .33 .63 .19 .08 .00 4.6 5.7 4.0 1.2 .3 H - N 10 10 10 10 10 10 10 10 10 10 10 Table 19 Summary of Clinical Hematology Data Males Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm RBC HGB HCT MCV MCH MCHC PLT XIOV/iL G/DL % FL PG % XlOVL 0 MEAN S.D. N 1 MEAN S.D. N 8.20 .530 10 8.42 .439 10 15.2 .78 10 15.2 .71 10 42.6 2.22 10 43.4 1.97 10 52.0 1.89 10 51.6 2.54 10 18.5 .69 10 18.1 1.04 10 35.6 .24 10 35.2 * .38 10 1171 158 10 1131 185 10 Cvance 6329-228 3M T6316.1 WBC XIO'/^L Table 19 Summary of Clinical Hematology Data Males Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS N-SEG LYMPH X10V/iL XIOVAiL MONO EOSIN BASO X10V/iL XlOV/iL XIOViiL N-SEGt LYMPH% MONO* EOSIN* Covance 6329-228 3M T6316.1 BASO* 0 MEAN 6.8 2.0 3.9 .8 .1 .0 29 57 11 2 0 S.D. 1.64 .73 1.29 .34 .05 .03 8.6 10.4 4.7 .9 N 10 10 10 10 10 10 10 10 10 10 10 1 MEAN 6.7 2.0 3.8 .8 .1 .0 28 57 12 2 0 S.D. 2.12 1.17 .93 .29 .05 .00 7.3 6.7 2.7 .6 N 10 10 10 10 10 10 10 10 10 10 10 Cm rv C3 Table 19 Summary of Clinical Hematology Data Females Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS RBC HGB HCT MCV MCH MCHC PLT X10`/m L G/DL t FL PG XIOV^L MEAN S.D. N MEAN S.D. N 7.71 .362 10 7.78 .435 10 14.9 .50 10 14.9 .46 10 41.4 1.77 10 41.6 1.16 10 53.8 1.63 10 53.5 1.75 10 19.4 .67 10 19.2 .61 10 36.0 .46 10 35.7 .41 10 941 117.1 10 1053 * 102.5 10 Covance 6329-Z20 3M T6316.1 o o c- Table 19 Summary of Clinical Hematology Data Females Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS WBC XI07/iL N-SEG LYMPH XIOV^L XIOV^L MONO EOSIN BASO x i o V a<l XIOV^L XIOV^L N-SEG% LYMPH* MONO* EOSIN* Covance 6329-228 3M T6316.1 BASO* MEAN 5.2 1.4 3.2 .6 .1 .0 27 60 11 2 0 S.D. 1.64 .48 1.16 .13 .03 .00 4.7 5.8 2.3 .8 N 10 10 10 10 10 10 10 10 10 10 10 MEAN 5.1 1.3 3.2 .6 .1 .0 26 60 12 2 0 S.D. 2.18 .73 1.66 .24 .04 .03 8.6 9.5 4.6 .7 N 10 10 10 10 10 10 10 10 10 10 10 <r> b Ul Table 20 Summary of Clinical Chemistry Data Hales Week 4 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm PCOAO IU/G Covance 6329-212 3M T-6316.1 0 MEAN S.D. N 7 3.8 5 3 MEAN S.D. N 8 2.5 5 30 MEAN S.D. N 9 1.7 5 rs 100 MEAN 6 S.D. 1.9 N5 300 MEAN 14 * S.D. N 3.6 5 Table 20 Summary of Clinical Chemistry Data Females Week 4 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm PCOAO IU/G 0 MEAN S.D. N 3 MEAN S.D. N 30 MEAN S.D. N 100 MEAN S.D. N 300 MEAN S.D. 3 2.7 5 5 1.6 5 4 2.7 5 5 1.6 5 15 * 7.9 Covance 6329-212 3M T-6316.1 o C Co fs) m Table 21 Summary of Clinical Chemistry Data Males Week 4 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm PCOAO IU/G MEAN S.D. N MEAN S.D. N 7 2.6 5 7 3.8 5 Covance 6329-228 3M T-6316.1 fccroo Table 21 Summary of Clinical Chemistry Data Females Week 4 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm PCOAO IU/G 0 MEAN S.D. N 1 MEAN S.D. N 3 1.1 5 4 2.3 5 Covance 6329-228 3M T-6316.1 DOSE ppm Table 22 Summary of Clinical Chemistry Data Males Week 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS GLU MG/DL UN MG/DL CREAT MG/DL T PRO G/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL 0 MEAN 106 13 .8 6.7 4.6 2.2 S.D. 6.3 1.9 .13 .48 .19 .31 N 555555 .1 53 .08 13.3 55 300 MEAN S.D. N 94 * 18 * .7 7.1 5.5 * 1.6 * .5 * 6.2 2.2 .08 .61 .38 .30 .09 5555555 7* 5.2 5 Covance 6329-212 3M T-6316.1 O C- in O DOSE ppm Table 22 Summary of Clinical Chemistry Data Males Week 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS AST/SGOT ALT/SGPT IU/L IU/L GGT IU/L CA MG/DL I PHOS MG/DL NA MMOL/L K MMOL/L CL MMOL/L 0 MEAN S.D. N 300 MEAN S.D. 113 24.8 5 179 * 55.6 42 7.0 5 271 * 213.8 2 10.5 1.6 .21 55 1 10.4 1.2 .26 7.8 .79 5 7.7 .34 148 1.5 5 147 1.6 5.7 .26 5 5.6 .32 103 1 5 103 2 Covance 6329-212 3M T-6316.1 Table 22 Summary of Clinical Chemistry Data Males Week 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm PCOAO IU/G Covance 6329-212 3M T-6316.1 MEAN S.D. N 300 MEAN S.D. N 9 3.5 5 26 * 5.3 5 cc * Cj cn w DOSE ppm Table 22 Summary of Clinical Chemistry Data Females Week 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS GLU MG/DL UN HG/DL CREAT MG/DL T PRO G/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL 0 MEAN 109 13 .8 7.3 5.4 1.9 .3 69 S.D. 8.8 2.3 .11 .31 .29 .19 .09 16.2 N 5 5 5 5 5 5' 5 5 300 MEAN 86 * 22 * .8 7.7 6.2 * 1.5 .4 11 * S.D. 20.0 3.7 .10 1.00 .63 .40 .08 4.0 N 55 555 555 Covance 6329-212 3M T-6316.1 O *4 m CJ DOSE ppm Table 22 Summary of Clinical Chemistry Data Females Week 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS AST/SGOT ALT/SGPT IU/L IU/L GGT IU/L CA MG/DL I PHOS MG/DL NA MMOL/L K MMOL/L CL MMOL/L 0 MEAN S.D. N 300 MEAN S.D. N 129 21.6 5 145 78.4 5 42 19.8 5 94 67.7 5 1 11.1 1.1 .38 55 1 11.2 1.7 .40 55 8.4 1.99 5 8.6 1.22 5 148 2.3 5 149 2.8 5 5.9 .61 5 5.6 .34 5 103 2 5 105 1 5 Covance 6329-212 3M T-6316.1 Table 22 Summary of Clinical Chemistry Data Females Week 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE PPm PCOAO IU/G 0 MEAN S.D. N 300 MEAN S.D. N 3 2.7 5 26 * 6.6 5 Covance 6329-212 3M T-6316.1 o c-. iw m m DOSE ppm Table 23 Summary of Clinical Chemistry Data Males Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS GLU MG/DL UN MG/DL CREAT MG/DL T PRO G/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL Covance 6329-212 3M T-6316.1 0 MEAN 111 13 .7 7.4 5.0 2.4 .2 82 S.D. 18.0 1.4 .13 .32 .16 .23 .12 14.4 N 10 10 10 10 10 10 10 10 3 MEAN 101 14 .7 7.1 4.7 * 2.4 .2 61 * S.D. 5.7 2.2 .06 .28 .14 .21 .08 6.8 N 10 10 10 10 10 10 10 10 30 MEAN 102 14 .7 7.1 4.8 2.4 .1 53 * S.D. 8.6 2.0 .14 .36 .18 .31 .10 7.9 N 10 10 10 10 10 10 10 10 'O 100 MEAN 94 * 15 .6 7.2 5.1 2.2 .2 29 * c S.D. 7.0 1.6 .05 .31 .25 .25 .08 9.8 C.1 N 10 10 10 10 10 10 10 10 01 o DOSE ppm Table 23 Summary of Clinical Chemistry Data Males Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS AST/SGOT ALT/SGPT IU/L IU/L GGT IU/L CA MG/DL I PHOS MG/DL NA MMOL/L K MMOL/L CL MMOL/L Covance 6329-212 3M T-6316.1 0 MEAN S.D. N 128 32.7 10 41 6.9 10 0 .4 10 10.8 .37 10 7.7 .62 10 149 1.7 10 6.5 .41 10 105 1.3 10 3 MEAN S.D. N 130 27.0 10 42 6.1 10 0 .7 10 10.6 .28 10 7.8 .69 10 148 1.7 10 6.2 .28 10 104 2.4 10 30 MEAN S.D. N 116 31.0 10 46 14.2 10 0 .3 10 10.8 .28 10 7.8 .55 10 148 1.5 10 6.1 * .34 10 106 2.1 10 100 MEAN 111 47 0 10.6 7.7 148 6.1 * 106 o S.D. N 32.3 11.1 1.3 .35 .42 1.7 .35 2.4 10 10 10 10 10 10 10 10 at - Hi DOSE ppm Table 23 Summary of Clinical Chemistry Data Females Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS GLU MG/DL UN MG/DL CREAT MG/DL T PRO G/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL Covance 6329-212 3M T-6316.1 0 MEAN 106 13 .8 8.3 6.1 2.2 .2 95 S.D. 10.4 1.5 .12 .47 .43 .21 .10 29-6 N 10 10 10 10 10 10 10 10 3 MEAN 108 14 .7 8.0 6.0 2.0 .2 80 S.D. 7.9 2.3 .10 .39 .43 .12 .18 12.9 N 10 10 10 10 10 10 10 10 30 MEAN 97 16 .8 7.8 5.8 2.0 .1 69 * S.D. 11.9 3.8 .16 .53 .37 .24 .13 18.3 N 10 10 10 10 10 10 10 10 100 MEAN 94 * 19 * .7 8.1 6.1 2.0 .2 58 * S.D. 9.9 4.3 .12 .31 .19 .32 .12 22.9 O N 10 10 10 10 10 10 10 10 C Cj in 00 DOSE Table 23 Summary of Clinical Chemistry Data Females Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS AST/SGOT ALT/SGPT IU/L IU/L GGT IU/L CA MG/DL I PHOS MG/DL NA MMOL/L K MMOL/L CL MMOL/L Covance 6329-212 3M T-6316.1 MEAN S.D. N 110 30.2 10 39 18.5 10 0 1.0 10 11.3 .37 10 7.0 1.22 10 149 2.4 10' 6.0 .80 10 106 2.0 10 MEAN S.D. N 96 14.8 10 32 6.5 10 0 .5 10 11.4 .38 10 7.3 .95 10 148 1.8 10 6.1 .59 10 105 1.2 10 MEAN S.D. N 118 16.0 10 34 6.0 10 0 .5 10 10.8 * .46 10 6.8 1.00 10 149 2.2 10 5.8 .35 10 106 1.4 10 100 MEAN S.D. N 87 17.1 10 33 4.7 10 1 1.5 10 11.1 .27 10 6.8 .74 10 149 2.0 10 5.9 .37 10 106 2.2 10 o V r * C/f C3 Table 23 Sunanary of Clinical Chemistry Data Males Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm PCOAO IU/G 0 MEAN S.D. N 3 MEAN S.D. N 30 MEAN S.D. N 100 MEAN S.D. 7 .9 5 7 1.4 5 5 1.6 5 6 2.7 Covance 6329-212 3M T-6316.1 Table 23 Summary of Clinical Chemistry Data Females Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE PPm PCOAO IU/G Covance 6329-212 3M T-6316.1 0 MEAN S.D. N 4 1.9 5 3 MEAN S.D. N 5 1.6 5 o 30 o C-3-) MEAN S.D. N 4 2.9 5 to m 100 MEAN 8 S.D. 3.3 N5 6 > W 0 ') to DOSE ppm Table 24 Summary of Clinical Chemistry Data Males Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS GLU MG/DL UN MG/DL CREAT MG/DL T PRO G/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL Covance 6329-228 3M T-6316.1 MEAN 113 14 .7 6.9 4.8 2.1 .0 59 S.D. 9.6 2.1 .10 .40 .22 .28 .05 7.0 N 10 10 10 10 10 10 10 10 MEAN 114 13 .6 7.0 4.7 2.3 .0 63 S.D. 7.4 2.1 .11 .32 .19 .26 .05 12.8 N 10 10 10 10 10 10 10 10 DOSE Table 24 Summary of clinical Chemistry Data Hales Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS AST/SGOT ALT/SGPT IU/L IU/L GGT IU/L CA MG/DL I PHOS MG/DL NA MMOL/L K MMOL/L CL MMOL/L Covance 6329-228 3M T-6316.1 MEAN S.D. N MEAN S.D. N 110 21.4 10 111 14.9 10 39 8.1 10 48 21.2 10 2 2.1 10 3 2.2 10 10.6 .19 10 10.6 .24 10 7.3 .63 10 7.0 .31 10 147 .8 10 147 .8 10 5.9 .39 10 6.3 * .31 10 106 1.9 10 107 .6 10 o o C' N? & a DOSE ppm Table 24 Summary of Clinical Chemistry Data Females Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS GLU MG/DL UN MG/DL CREAT MG/DL T PRO G/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL 0 MEAN 114 14 .8 8.2 6.1 2.1 .2 94 S.D. 8.7 2.3 .14 .60 .52 .22 .16 22.1 N 10 10 10 10 10 10 10 10 1 MEAN 110 15 .7 7.9 5.8 2.1 .1 88 S.D. 3.8 2.4 .12 .68 .66 .27 .13 20.3 N 10 10 10 10 10 10 10 10 Covance 6329-228 3M T-6316.1 c- cn DOSE ppm Table 24 Summary of Clinical Chemistry Data Females Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS AST/SGOT ALT/SGPT IU/L IU/L GGT IU/L CA MG/PL I PHOS MG/DL NA MMOL/L K MMOL/L CL MMOL/L 0 MEAN S.D. N 1 MEAN S.D. 110 58.6 10 108 24.1 44 24.9 10 44 19.1 2 1.3 10 2 2.0 11.4 .54 10 11.2 .29 6.0 .44 10 6.4 * .43 145 1.6 10' 147 * .8 5.8 .38 10 6.0 .36 106 1.8 10 107 2.2 Covance 6329-228 3M T-6316.1 ) m Table 24 Summary of Clinical Chemistry Data Hales Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm PCOAO IU/G 0 MEAN S.D. N 1 MEAN S.D. N 6 1.3 5 5 1.1 5 Covance 6329-228 3M T-6316.1 Table 24 Summary of Clinical Chemistry Data Females Meek 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE pprn PCOAO IU/G Covance 6329-228 3M T-6316.1 MEAN S.D. N 3 .9 5 MEAN S.D. N 5 1.6 5 oo C'- cn DOSE ppm Table 25 Summary of Clinical Chemistry Data Males Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS GLU MG/DL UN MG/DL CREAT MG/DL T PRO G/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL Covance 6329-212 3M T6316.1 892T00 0 MEAN 106 11 .6 7.4 4.6 2.7 S.D. 11.8 1.2 .13 .31 .25 .13 N 10 10 10 10 10 10 .1 .13 10 3 MEAN 108 13 .7 7.4 4.8 2.6 S.D. 12.1 1.8 .12 .43 .39 .19 N 10 10 10 10 10 10 .2 .11 10 30 MEAN S.D. N 98 13 .7 7.2 4.7 2.5 * .1 6.2 1.7 .10 .51 .39 .20 .10 10 10 10 10 10 10 10 100 MEAN S.D. N 95 * 15 * .6 7.7 5.3 * 2.4 * .1 7.3 1.6 .11 .32 .25 .28 .11 10 10 10 10 10 10 10 96 30.3 10 86 19.8 10 68 * 18.8 10 42 * 16.2 10 DOSE ppm Table 25 Summary of Clinical Chemistry Data Males Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS AST/SGOT ALT/SGPT IU/L IU/L GGT IU/L CA MG/DL I PHOS MG/DL NA MMOL/L K MMOL/L CL MMOL/L Covance 6329-212 3M T6316.1 0 MEAN S.D. N 114 28.0 10 40 13.8 10 i 1.3 10 10.8 .38 10 7.4 .82 10 149 1.9 10 6.7 .48 10 106 1 10 3 MEAN 119 42 0 10.6 6.9 147 6.2 105 S.D. 38.3 21.2 .7 .39 .66 2.3 .48 2 o N 10 10 10 10 10 10 10 10 o c*< 30 MEAN 114 57 1 10.6 7.2 147 6.3 106 S.D. 38.7 31.0 1.0 .34 .40 1.4 .37 1 CD N 10 10 10 10 10 10 10 10 C 3 100 MEAN 165 94 1 10.7 7.2 147 6.3 106 S.D. 192.1 113.7 .8 .32 .40 1.4 .35 1 N 10 10 10 10 10 10 10 10 00 Co 4V-.^ o DOSE ppm Table 25 Summary of Clinical Chemistry Data Females Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS GLU MG/DL UN MG/DL CREAT MG/DL T PRO G/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL 0 MEAN 101 15 .8 8.0 5.8 2.2 S.D. 13.4 3.7 .24 .65 .53 .23 N 10 10 10 10 10 10 3 MEAN 103 14 .7 8.2 5.8 2.4 S.D. 7.3 2.2 .11 .54 .57 .28 N 10 10 10 10 10 10 30 MEAN 107 16 .6 8.0 5.8 2.2 S.D. 8.9 3.7 .08 .61 .41 .28 N 10 10 10 10 10 10 100 MEAN S.D. N 92 21 * .8 8.5 6.2 2.4 7.1 7.0 .21 .50 .30 .25 10 10 10 10 10 10 .2 87 .10 14 10 10 .2 104 .14 21 10 10 .2 86 .12 19 10 10 .2 71 .14 20 10 10 Covance 6329-212 3M T6316.1 DOSE ppm Table 25 Summary of Clinical Chemistry Data Females Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS AST/SGOT ALT/SGPT IU/L IU/L GGT IU/L CA MG/DL I PHOS MG/DL NA MMOL/L K MMOL/L CL MMOL/L Covance S329-212 3M T6316.1 0 MEAN S.D. N 122 30.0 10 44 19.3 10 1 1.1 10 11.0 .41 10 6.0 .44 10 147 2.9 10 6.1 .57 10 105 2 10 3 MEAN S.D. N 102 23.6 10 41 17.7 10 2 4.7 10 11.0 .41 10 5.8 .76 10 147 .9 10 5.7 .54 10 105 1 10 30 MEAN S.D. N 104 22.1 10 37 10.8 10 2 1.4 10 11.0 .53 10 6.1 .92 10 147 1.7 10 6.1 .69 10 107 1 10 100 MEAN 105 47 1 11.2 6.2 146 5.8 104 U S.D. 42.4 17.3 1.3 .24 .63 1.6 .71 2 N 10 10 10 10 10 10 10 10 CO Table 26 Summary of Clinical Chemistry Data Males Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS GLU MG/DL UN MG/DL CREAT MG/DL T PRO G/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL Covance 6329-228 3M T6316.1 MEAN 103 12 .6 6.9 4.6 2.2 .1 75 S.D. 10.1 1.2 .06 .32 .17 .28 .05 12.5 N 10 10 10 10 10 10 10 10 MEAN 111 * 13 .6 7.1 4.6 2.5 .2 84 o S.D. 7.3 .9 .04 .29 .18 .25 .05 17.1 o N 10 10 10 10 10 10 10 10 Cm fv vi DOSE ppm Table 26 Summary of Clinical Chemistry Data Males Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS AST/SGOT ALT/SGPT IU/L IU/L GGT IU/L MG/DL I PHOS MG/DL NA MMOL/L K MMOL/L CL MMOL/L Covance 6329-228 3M T6316.1 0 MEAN S.D. N 120 21.8 10 43 23.3 10 2 .8 10 10.6 .16 10 7.0 .41 10 146 1.2 10 5.9 .34 10 105 2 10 1 MEAN 110 45 1 10.8 * 7.0 147 6.3 * 105 S.D. 20.4 18.1 .8 .20 .47 1.1 .29 2 N 10 10 10 10 10 10 10 10 o C4 vi CO DOSE ppm Table 26 Summary of Clinical Chemistry Data Females Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS GLU MG/DL UN MG/DL CREAT MG/DL T PRO G/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL Covance 6329-228 3M T6316.1 0 MEAN 106 14 .7 8.3 6.1 2.2 .2 117 S.D. 8.0 1.7 .03 .41 .32 .23 .00 27.5 N 10 10 10 10 10 10 , 10 10 1 MEAN 104 13 .7 * 8.2 5.9 2.3 .2 111 S.D. 7.2 2.2 .05 .45 .64 .50 .06 33.8 o N 10 10 10 10 10 10 10 10 Cj si DOSE ppm Table 26 Summary of Clinical Chemistry Data Females Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS AST/SGOT ALT/SGPT IU/L IU/L GGT IU/L CA MG/DL I PHOS MG/DL NA MMOL/L K MMOL/L CL MMOL/L Covance 6329-228 3M T6316.1 0 MEAN S.D. N 115 50.2 10 58 38.8 10 2 1.9 10 11.3 .38 10 5.6 .59 10 144 1.3 10 5.2 .50 10 103 2 10 1 MEAN 178 96 4 11.2 6.1 146 * 5.5 104 S.D. 168.9 113.8 6.3 .43 .59 1.3 .54 2 N 10 10 10 10 10 10 10 10 <-*> 6 to to *\i to  c* d DOSE ppm Table 27 Summary of Clinical Chemistry Data Males Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS GLU MG/DL UN MG/DL CREAT MG/DL T PRO G/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL 0 MEAN 115 13 .6 7.3 4.4 2.9 S.D. 11.7 1.5 .10 .38 .25 .45 N 10 10 10 10 10 10 ,i 146 .10 70.8 10 10 3 MEAN 111 12 .6 7.6 4.7 2.9 S.D. 10.8 1.6 .11 .67 .49 .48 N 10 10 10 10 10 10 .2 119 .13 36.1 10 10 30 MEAN 116 14 .7 7.3 4.7 2.6 .2 92 * S.D. 9.4 2.2 .18 .32 .18 .35 .13 38.3 N 10 10 10 10 10 10 10 10 100 MEAN 113 14 .6 7.7 5.3 * 2.4 * .1 S.D. 13.7 1.0 .11 .35 .30 .27 .09 N 10 10 10 10 10 10 10 49 * 21.4 10 Covance 6329-212 3M T6316.1 oO Cu sai DOSE ppm Table 27 Summary of Clinical Chemistry Data Males Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS AST/SGOT ALT/SGPT IU/L IU/L GGT IU/L CA MG/DL I PHOS MG/DL NA MMOL/L K MMOL/L CL MMOL/L 0 MEAN S.D. N 3 MEAN S.D. N 30 MEAN S.D. N 100 MEAN S.D. N 117 23.7 10 108 22.9 10 105 25.4 10 83 * 16.2 10 40 9.4 10 48 14.4 10 51 19.2 10 68 * 26.5 10 4 3.5 10 11.1 .41 10 3 2.7 10 11.0 .35 10 4 4.5 10 10.9 .30 10 6 4.2 10 10.9 .20 10 6.5 .87 10 6.7 .53 10 6.8 .62 10 6.5 .37 10 150 1.7 10 150 1.6 10 150 1.9 10 148 * 1.6 10 6.4 .36 10 6.5 .52 10 6.5 .42 10 6.3 .45 10 109 2 10 108 1 10 109 1 10 109 2 10 Covance 6329-212 3M T6316.1 DOSE ppm Table 27 Summary of Clinical Chemistry Data Females Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS GLU MG/DL UN MG/DL CREAT MG/DL T PRO G/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL Covance 6329-212 3M T6316.1 0 MEAN 108 13 .7 8.0 5.9 2.1 S.D. 14.0 3.0 .12 .70 .58 .25 N 10 10 10 10 10 10' .3 116 .16 22.1 10 10 3 MEAN 105 13 .7 8.2 5.8 2.5 .2 119 S.D. 13.0 1.6 .17 .55 .57 .40 .19 39.1 r> N 10 10 10 10 10 10 10 10 30 C4 MEAN S.D. 93 * 14 .8 8.4 5.9 2.4 7.8 2.1 .09 .37 .22 .33 .3 94 .25 23.9 N 10 10 10 10 10 10 10 10 go 100 MEAN 103 16 .6 8.5 5.9 2.6 * .1 88 S.D. 11.3 4.2 .08 .42 .22 .37 .12 23.2 N 10 . 10 10 10 10 10 10 10 DOSE ppm Table 27 Summary of Clinical Chemistry Data Females Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESUIiFONAMIDO ETHANOL IN RATS AST/SGOT ALT/SGPT IU/L IU/L GGT IU/L CA MG/DL I PHOS MG/DL NA MMOL/L K MMOL/L CL MMOL/L Covance 6329-212 3M T6316.1 0 MEAN S.D. N 107 33.7 10 43 16.5 10 2 1.8 10 li.i .44 10 5.8 .73 10 148 2.2 10 5.6 .51 10 106 3 10 3 MEAN 104 48 2 11.2 5.8 149 6.1 108 S.D. N 27.4 10 22.0 10 2.8 10 .43 10 .44 1.7 .45 2 10 10 10 10 30 MEAN 125 66 5 11.1 6.2 150 5.9 107 S.D. 56.9 72.0 6.8 .42 .85 3.9 .56 2 CD N 10 10 10 10 10 10 10 10 100 MEAN S.D. N 89 19.9 10 63 26.1 10 4 4.0 10 11.3 .29 10 5.8 .48 10 147 1.9 10 5.7 .40 10 107 2 10  O Co K CJ o Table 28 Summary of Clinical Chemistry Data Males Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS GLU MG/DL UN MG/DL CREAT MG/DL T PRO G/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL Covance 6329-228 3M T6316.1 MEAN S.D. N MEAN S.D. N 119 19.5 10 127 13.2 10 12 2.0 10 12 2.3 10 .7 .09 10 .7 .03 10 7.1 .35 10 7.1 .31 10 4.6 .37 10 4.6 .31 10 2.5 .29 10 ' 2.5 .30 10 .2 .05 10 .2 .05 10 105 33.8 10 102 42.1 10 DOSE ppm Table 28 Summary of Clinical Chemistry Data Males Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS AST/SGOT ALT/SGPT IU/L IU/L GGT IU/L CA MG/DL I PHOS MG/DL NA MMOL/L K MMOL/L CL MMOL/L Covance 6329-228 3M T6316.1 0 MEAN S.D. N 108 55.4 10 77 118.8 10 2 1.1 10 10.3 .55 10 6.2 .89 10 149 3.5 10 ' 6.1 .58 10 106 3 10 1 MEAN 110 42 1 10.5 6.4 150 6.4 107 S.D. 40.0 12.3 1.3 .34 .53 4.3 .50 3 O N 10 10 10 10 10 10 10 10 O Cj QD o e Co ?v GO W Table 28 Summary of Clinical Chemistry Data Females Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS GLU MG/DL UN MG/DL CREAT MG/DL T PRO G/DL ALB G/DL GLOB G/DL T BILI MG/DL CHOL MG/DL Covance 6329-228 3M T6316.1 MEAN 113 11 .8 8.4 5.9 2.4 .3 142 S.D. 17.5 1.1 .07 .44 .36 .34 .22 34.0 N 10 10 10 10 10 10 10 10 MEAN 108 14 * .8 8.5 5.8 2.7 S.D. 8.9 2.5 .07 .47 .40 .41 N 10 10 10 10 10 10 .2 126 .06 20.8 10 10 DOSE ppm Table 28 Summary of Clinical Chemistry Data Females Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS AST/SGOT ALT/SGPT IU/L IU/L GGT IU/L CA MG/DL I PHOS MG/DL NA MMOL/L K MMOL/L CL MMOL/L Covance 6329-228 3M T6316.1 MEAN S.D. N 100 29.1 10 54 24.7 10 2 1.0 10 11.1 .44 10 5.4 1.05 10 147 4.1 10 5.4 .37 10 102 2.6 10 MEAN 156 72 2 11.0 5.7 147 5.3 104 S.D. 160.1 62.1 2.6 .50 1.14 2.3 .68 3.0 oo N 10 10 10 10 10 10 10 10 C-' 00 CO Table 29 Summary of Clinical Urinalysis Data Males Week 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN FATS DOSE ppm U VOL ML SP GR U PH Covance 6329-212 3M T-6316.1 0 MEAN 17.5 1.020 S.D. 10.64 .0086 N 55 7.0 .35 5 300 MEAN 28.5 1.010 6.9 S.D. 10.88 .0047 .22 o N 5 55 o c- r, 00 & Table 29 Summary of Clinical Urinalysis Data Females Week 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm U VOL ML SP GR U PH Covance 6329-212 3M T-6316.1 0 MEAN 19.9 1.012 S.D. 17.75 .0102 N 55 6.8 .27 5 300 o MEAN S.D. 21.6 12.63 1.010 .0051 6.7 .45 C N 555 C4 to CO Tabla 30 Summary of Clinical Urinalysis Data Males Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm U VOL ML SP GR U PH Covance 6329-212 3M T-6316.1 0 MEAN S.D. N 18.7 14.79 10 1.024 .0151 10 6.9 .39 10 3 MEAN 25.1 1.015 6.9 S.D. 15.47 .0079 .21 oo N 10 10 10 30 MEAN 15.8 1.022 6.8 S.D. 9.53 .0109 .26 N 10 10 10 GO 100 MEAN 19.9 1.022 6.8 S.D. 15.56 .0160 .35 N 10 10 10 Table 30 Summary of Clinical Urinalysis Data Females Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm U VOL ML SP GR U PH Covance 6329-212 3M T-6316.1 0 MEAN S.D. N 19.0 13.21 10 1.012 .0082 10 6.7 .26 10 3 MEAN 22.2 1.012 S.D. 16.26 .0102 N 10 10 6.8 ,26 10 6 30 Cu MEAN 19.2 1.014 6.6 S.D. 16.50 .0109 .39 N 10 10 10 G3 100 MEAN S.D. N 23.2 16.13 10 1.012 .0086 10 6.6 .16 10 Table 31 Summary of Clinical Urinalysis Data Males Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm U VOL ML SP GR U PH Covance 6329-228 3M T-6316.1 0 MEAN 22.7 1.015 S.D. 15.31 .0116 N 10 10 7.1 .32 10 1 MEAN 21.9 1.017 7.2 6 S.D. N 13.22 10 .0085 10 .24 10 e- GO G5 Table 31 Summary of Clinical Urinalysis Data Females Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE PPm U VOL ML SP GR U PH Covance 6329-228 3M T-6316.1 0 MEAN 12.5 1.017 S.D. 13.88 .0078 N 10 10 6.8 .26 10 1 MEAN 22.0 1.012 S.D. 18.74 .0112 N 10 10 6.7 .26 10 c. GO CD Table 32 Summary of Clinical Urinalysis Data Males Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm U VOL ML SP GR U PH Covance 6329-212 3M T6316.1 0 MEAN 15.5 1.024 S.D. 8.17 .0087 N 10 10 7.0 .33 10 3 MEAN 13.7 1.025 S.D. 5.65 .0090 N 10 10 7.2 .35 10 30 MEAN S.D. N 13.7 13.33 10 1.030 .0137 10 7.0 .33 10 o 100 MEAN 15.4 1.025 7.0 C-j S.D. N 13.36 10 .0100 10 .33 10 o e o c** u CO Table 32 Summary of Clinical Urinalysis Data Females Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm U VOL ML SP GR U PH 0 MEAN S.D. N 3 MEAN S.D. N 30 MEAN S.D. N 100 MEAN S.D. N 18.1 16.96 10 15.9 15.38 10 13.0 13.35 10 13.2 8.01 10 1.019 .0140 10 1.016 .0100 10 1.020 .0190 10 1.019 .0081 10 6.9 .32 10 6.8 .41 10 6.7 .35 10 6.8 .49 10 Covance 6329-212 3M T6316.1 Table 33 Summary of Clinical Urinalysis Data Males Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm U VOL ML SP GR U PH 0 MEAN S.D. N 13.8 12.00 10 1.022 .0081 10 1 MEAN 15.4 1.026 S.D. 13.27 .0125 N 10 10 6.9 .39 10 7.0 .28 10 Covance 6329-228 3M T6316.1 Table 33 Summary of Clinical Urinalysis Data Females Meek 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm U VOL ML SP GR U PH Covance 6329-228 3M T6316.1 0 MEAN 17.1 1.019 S.D. 17.79 .0134 N 10 10 1 MEAN S.D. N 20.0 17.04 10 1.018 .0126 10 6.6 .21 10 6.6 .16 10 O O C4 CD CO O w pV-. j1 IQ w Table 34 Summary o Clinical Urinalysis Data Hales Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm U VOL ML SP GR U PH 0 MEAN S.D. N 3 MEAN S.D. N 30 MEAN S.D. N 100 MEAN S.D. N 19.3 15.90 10 13.7 6.72 10 14.7 7.46 10 17.3 12.78 10 1.024 .0092 10 1.029 .0106 10 1.026 .0078 10 1.023 .0110 10 6.8 .26 10 7.0 .33 10 6.8 .35 10 6.8 .34 10 Covance S329-212 3M T6316.1 Table 34 Summary of Clinical Urinalysis Data Females Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm U VOL ML SP GR U PH 0 MEAN S.D. N 3 MEAN S.D. N 30 MEAN S.D. N 100 MEAN S.D. N 13.1 7.81 10 19.3 17.82 10 13.7 12.84 10 17.0 14.38 10 1.018 .0074 10 1.018 .0122 10 1.021 .0101 10 1.021 .0131 10 6.4 .28 10 6.4 .16 10 6.4 .24 10 6.4 .16 10 Covance 6329-212 3M T6316.1 Table 35 Summary of Clinical Urinalysis Data Males Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1*) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm U VOL ML SP GR U PH 0 MEAN S.D. N 1 MEAN S.D. N 14.7 12.83 10 1.026 .0110 10 10.5 6.47 10 1.030 .0123 10 7.0 .33 10 6.9 .21 10 Covance 6329-228 3M T6316.1 oo c ,i N w Table 35 Summary of Clinical Urinalysis Data Females Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm U VOL ML SP GR U PH 0 MEAN S.D. N 1 MEAN S.D. N 27.2 19.88 10 13.0 10.19 10 1.014 .0106 10 1.019 .0093 10 6.5 .24 10 6.5 .33 10 Covance 6329-228 3M T6316.1 <*> w Table 36 summary of Urine Chemistry Data Males Week 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm U NA MMOL/L U K NA EXC MMOL/L MMOL K EXC MMOL 0 MEAN S.D. N 300 MEAN S.D. N 27 13.5 5 20 14.2 5 120 36.4 5 41 * 18.8 5 .37 .135 5 .53 .213 4 1.99 1.394 5 1.10 .403 4 Covance 6329-212 3M T-6316.1 rs O C-s fg 06 Table 36 Summary of Urine Chemistry Data Females Meek 8 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm U NA MMOL/L U K NA EXC MMOL/L MMOL K EXC MMOL 0 MEAN S.D. N 300 MEAN S.D. N 16 11.4 5 20 9.9 5 53 47.3 5 42 18.0 5 .15 .074 4 .38 .178 4 .46 .164 4 .76 * .113 4 Covance 6329-212 3M T-6316.1 oo CO CD C03300 Table 37 Summary of Urine Chemistry Data Males Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm U NA MMOL/L U K NA EXC MMOL/L MMOL K EXC MMOL 0 MEAN S.D. N 3 MEAN S.D. N 30 MEAN S.D. N 100 MEAN S.D. N 28 15.9 10 22 14.5 10 30 24.4 10 29 28.2 10 155 111.3 10 82 51.3 10 127 88.4 10 128 115.5 10 .35 .159 10 .40 .197 10 .32 .108 10 .31 .071 10 1.70 .469 10 1.40 .521 10 1.44 .405 10 1.35 .335 10 Covance 6329-212 3H T-6316.1 tocroo Table 37 Summary of Urine Chemistry Data Females Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm U NA MMOL/L U K NA EXC MMOL/L MMOL K EXC MMOL 0 MEAN S.D. N MEAN S.D. N MEAN S.D. N MEAN S.D. N 19 12.0 10 21 16.5 10 18 11.0 10 20 15.4 10 61 47.7 10 70 73.0 10 73 76.5 10 57 51.3 10 .25 .062 10 .26 .139 10 .22 .132 10 .31 .098 10 .74 .256 10 .66 .214 10 .65 .244 10 .72 .271 10 Covance 6329-212 3M T-6316.1 Table 38 summary o Urine Chemistry Data Hales Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm U NA MMOL/L U K NA EXC MMOL/L MMOL K EXC MMOL 0 MEAN S.D. N MEAN S.D. N 24 16.7 10 30 14.3 10 80 72.9 10 89 43.9 10 .35 .079 9 .51 * .151 9 1.06 .227 9 1.53 * .425 9 Covance 6329-228 3M T-6316.1 <" > C Co o iO corroo Table 38 Summary of Urine Chemistry Data Females Week 14 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm U NA MMOL/L U K NA EXC MMOL/L MMOL K EXC MMOL 0 MEAN S.D. N 1 MEAN S.D. N 26 12.0 10 18 12.0 10 76 34.6 10 51 41.3 10 .22 .119 9 .22 .126 8 .64 .273 9 .56 .333 8 Covance 6329-228 3M T-6316.1 Table 39 Summary of Urine Chemistry Data Hales Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm U NA MMOL/L U K NA EXC MMOL/L MMOL K EXC MMOL Covance 6329-212 3M T6316.1 MEAN S.D. N 28 15.9 10 102 47.8 10 .37 .227 10 1.30 .281 10 MEAN S.D. N 34 11.2 10 102 34.0 10 .46 .227 10 1.26 .274 10 30 MEAN S.D. N 46 20.5 10 135 76.2 10 .43 .197 10 1.12 .266 10 100 MEAN 43 114 .46 1.26 S.D. 25.3 56.2 .186 .413 oo N 10 10 9 9 Co Co o  c,'. 4 o t/1 Table 39 Summary of Urine Chemistry Data Females Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%| N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm U NA MMOL/L U K NA EXC MMOL/L MMOL K EXC MMOL 0 MEAN S.D. N 3 MEAN S.D. N 30 MEAN S.D. N 100 MEAN S.D. N 23 16.1 10 29 22.9 10 21 10.7 9 27 20.8 10 85 58.0 10 66 43.7 10 52 20.2 9 78 38.4 10 .24 .164 8 .26 .148 9 .24 .133 8 .27 .098 10 .81 .318 8 .61 .173 9 .57 .229 8 .81 .191 10 Covance 6329-212 3M T6316.1 oo u o m Table 40 Summary of Urine Chemistry Data Males Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS U NA MMOL/L U K NA EXC MMOL/L MMOL K EXC MMOL MEAN S.D. N MEAN S.D. N 18 9.3 10 26 18.7 10 112 52.8 10 151 123.3 10 .18 .062 10 .24 .135 9 1.11 .383 10 1.34 .363 9 Covance 6329-228 3M T6316.1 Table 40 Summary of Urine Chemistry Data Females Week 27 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm U NA MMOL/L U K NA EXC MMOL/L MMOL K EXC MMOL 0 MEAN S.D. N 1 MEAN S.D. N 16 16.3 10 10 7.1 9 101 83.6 10 70 47.4 9 .08 .025 8 .14 * .057 9 .61 .212 8 .96 * .383 9 Covance 6329-228 3M T6316.1 oo c- uo Table 41 Summary of Urine Chemistry Data Males Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm U NA MMOL/L U K NA EXC MMOL/L MMOL K EXC MMOL Covance 6329-212 3M T6316.1 0 MEAN S.D. N 3 MEAN S.D. N 30 MEAN S.D. N 100 MEAN S.D. N 23 9.5 10 27 7.2 10 28 11.2 10 28 12.5 10 110 52.5 10 141 60.8 10 136 61.5 10 116 58.9 10 .28 .077 8 .33 .102 10 .39 .221 10 .38 .142 9 1.50 .296 8 1.62 .273 10 1.63 .462 10 1.45 .335 9 Co oC O OS <r> o C-1. cCs> Table 41 Summary of Urine Chemistry Data Females Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm U NA MMOL/L UK MMOL/L NA EXC MMOL K EXC MMOL 0 3 30 100 MEAN S.D. N MEAN S.D. N MEAN S.D. N MEAN S.D. N 29 16.8 10 29 23.4 10 24 12.6 10 31 27.4 10 88 40.9 10 92 68.9 10 101 60.4 10 113 82.0 10 .33 .206 10 .26 .086 8 .24 .107 9 .28 .107 10 .91 .240 10 .87 .283 8 .98 .395 9 1.01 .329 10 Covance 6329-212 3M T6316.1 Table 42 Summary of Urine Chemistry Data Males Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm U NA MMOL/L U K NA EXC MMOL/L MMOL K EXC MMOL 0 MEAN S.D. N 1 MEAN S.D. N 20 13.0 10 26 17.9 10 143 68.2 10 165 88.6 10 .23 .135 9 .20 .100 10 1.61 .579 9 1.29 .256 10 Covance 6329-228 3M T6316.1 O Co ti H* O Table 42 Summary of Urine Chemistry Data Females Week 53 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) M-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS DOSE ppm U NA MMOL/L U K NA EXC MMOL/L MMOL K EXC MMOL 0 MEAN S.D. N 1 MEAN S.D. N 13 9.6 10 20 14.9 10 64 48.6 10 118 101.1 10 .25 .191 6 .18 .085 10 .93 .569 6 .93 .290 10 Covance 6329-228 3M T6316.1 O . C` Q H* TABLE 43 Summary of Organ Height Data Week 4 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 1 TABLE INCLUDES: SEX=ALL;GR0UP=1,2,3,4,5;WEEKS=ALL DEATH=1 ;SUBSET=T LIVER SEX DOSE GROUP M1 NUMBER IN GROUP; MEAN: STANDARD DEV: M2 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT <g) ORGAN WEIGHT (g) 5 350.7 33.3 5 10.8223 1.5753 5 362.8 32.5 5 10.7856 1.1739 ORGAN-TOBODY WT (%) 5 3.0744 0.1520 ORGAN-TO- BRAIN WT RATIO SEX DOSE GROUP F1 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 2.9699 0.1104 F2 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) 5 196.1 20.3 5 209.0 29.8 ORGAN WEIGHT (g) ORGAN-TOBODY WT (%) ORGAN-TO- BRAIN WT RATIO 5 6.4594 0.4155 5 3.3170 0.3676 5 6.7264 0.7320 5 3.2329 0.1459 M3 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 344.4 28.5 5 10.9453 1.5396 5 3.1683 0.2257 F3 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 198.8 17.2 5 6.8324 0.6354 5 3.4373 0.1362 TABLE 43 Summary of Organ Weight Data Week 4 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 2 TABLE INCLUDES: SEX=ALL;GROUP=l,2,3,4,5;WEEKS=ALL DEATH=1 ;SUBSET=T LIVER SEX DOSE GROUP M4 TERMINAL BODY WT (g) ORGAN WEIGHT (g) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO SEX DOSE GROUP F4 TERMINAL BODY WT (g) ORGAN WEIGHT (g) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO NUMBER IN GROUP MEAN STANDARD DEV M5 NUMBER IN GROUP MEAN STANDARD DEV 5 343.8 36.4 5 12.2371 0.9341 5 3.5708 0.2090 5 310.0 28.3 5 15.7639 1.4058 5 5.0860 0.1240 NUMBER IN GROUP: MEAN: STANDARD DEV: F5 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 191.3 13.1 5 7.1709 0.5706 5 181.6 15.1 5 9.2338 1.4896 5 3.7577 0.3444 5 5.1048 0.8198 TABLE 44 Summary of Organ Weight Data Week 4 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 1 TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=1;SUBSET=T LIVER SEX DOSE GROUP M8 NUMBER IN GROUP MEAN STANDARD DEV TERMINAL BODY WT (g) ORGAN WEIGHT (g) 5 314.0 18.3 5 11.5023 1.4141 ORGAN-TOBODY WT (%) 5 3.6573 0.3062 ORGAN-TOBRAIN WT RATIO SEX DOSE GROUP F8 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT <g) 5 199.2 14.9 ORGAN WEIGHT (g) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 75.7008 0.9577 35.8600 0.2780 M9 NUMBER IN GROUP MEAN STANDARD DEV 5 308.7 14.1 5 11.1683 0.9088 5 3.6223 0.3092 F9 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 218.0 20.1 5 8.1095 1.1330 5 3.7211 0.3797 6 Co to TABLE 45 Summary of Organ Weight Data Week 8 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE {98.1%) N-ETHYL PERFLUOROOCTANBSULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 1 TABLE INCLUDES: SEX=ALL;GROUP=l,5;WEEKS=ALL DEATH=2;SUBSET=ALL LEFT ADRENAL SEX DOSE GROUP TERMINAL BODY WT (0) ORGAN WEIGHT (0) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO SEX DOSE GROUP TERMINAL BODY WT (0) ORGAN WEIGHT (0) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO H1 F1 NUMBER IN GROUP MEAN STANDARD DEV 5 442.3 31.8 5 0.0318 0.0042 5 0.0072 0.0013 5 0.0146 0.0020 NUMBER IN GROUP MEAN STANDARD DEV 5 257.4 31.4 5 0.0400 0.0077 5 0.0155 0.0018 5 0.0198 0.0036 O M5 F5 NUMBER IN GROUP 55 5 5 NUMBER IN GROUP 5 5 5 5 MEAN 317.3* 0.0278 0.0088 0.0136 MEAN 170.3* 0.0304* 0.0179 0.0167 Mi STANDARD DEV 50.6 0.0056 0.0018 0.0029 STANDARD DEV 14.1 0.0043 0.0021 0.0023 o o C.-j Co to TABLE 45 Summary of Organ Weight Data Week 8 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 2 TABLE INCLUDES: SEX=ALL;GR0UP=1,5;WEEKS=ALL DEATH=2;SUBSET=ALL RIGHT ADRENAL SEX DOSE GROUP M1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) 5 442.3 31.8 ORGAN WEIGHT (g) 5 0.0295 0.0029 ORGAN-TOBODY WT (%) 5 0.0067 0.0011 ORGAN-TOBRAIN WT RATIO 5 0.0136 0.0014 SEX DOSE GROUP F1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY. WT (9) 5 257.4 31.4 ORGAN WEIGHT (g) 5 0.0373 0.0060 ORGAN-TOBODY WT (%) ORGAN-TO BRAIN WT RATIO 5 0.0145 0.0014 5 0.0184 0.0029 M5 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 317.3* 50.6 5 0.0261 0.0064 5 0.0082 0.0013 5 0.0126 0.0024 F5 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 170.3* 14.1 5 0.0283 0.0078 5 0.0166 0.0043 5 0.0156 0.0045 TABLE 45 Summary of Organ Height Data Week 8 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3H T-6316.1 PAGE: 3 TABLE INCLUDES: SEX=ALL;GROUP=l,5;WEEKS=ALL DEATH=2;SUBSET=ALL BRAIN SEX DOSE GROUP TERMINAL BODY WT (g) ORGAN WEIGHT (9) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO SEX DOSE GROUP TERMINAL BODY WT (g) ORGAN WEIGHT (g) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO M1 F1 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 442.3 31.8 5 2.1728 0.0240 5 0.4934 0.0382 5 1.0000 0.0000 NUMBER IN GROUP MEAN STANDARD DEV 5 257.4 31.4 5 2.0222 0.0981 5 0.7919 0.0687 5 1.0000 0.0000 o o M5 c . NUMBER IN GROUP: C v MEAN: STANDARD DEV: 5 317.3* 50.6 5 2.0479 0.1770 5 0.6519* 0.0594 5 1.0000 0.0000 F5 NUMBER IN GROUP MEAN STANDARD DEV 5 170.3* 14.1 5 1.8176* 0.1064 5 1.0704* 0.0662 5 1.0000 0.0000 <1 TABLE 45 Summary of Organ Height Data Week 8 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 4 TABLE INCLUDES: SEX=ALL;GROUPS1,5;WEEKSsALL DEATH=2;SUBSET=ALL LEFT KIDNEY SEX DOSE GROUP M1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (?) 5 442.3 31.8 ORGAN WEIGHT (?) 5 1.4309 0.1350 ORGAN-TOBODY WT (%) 5 0.3240 0.0269 ORGAN-TOBRAIN WT RATIO 5 0.6586 0.0623 SEX DOSE GROUP F1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (?) 5 257.4 31.4 ORGAN WEIGHT (?) 5 0.9220 0.1189 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 5 0.3585 0.0268 5 0.4550 0.0456 H5 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 317.3* 50.6 5 1.4077 0.2404 5 0.4435* 0.0220 5 0.6856 0.0805 F5 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 170.3* 14.1 5 0.7874* 0.0384 5 0.4640* 0.0309 5 0.4338 0.0216 TABLE 45 Summary of Organ Weight Data Week 8 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 5 TABLE INCLUDES: SEX=ALL:GROUP=l,5;WEEKS=ALL DEATH=2;SUBSET=ALL RIGHT KIDNEY SEX DOSE GROUP M1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (0) 5 442.3 31.8 ORGAN WEIGHT (0) 5 1.4168 0.1080 ORGAN-TOBODY WT (%) 5 0.3207 0.0177 ORGAN-TOBRAIN WT RATIO 5 0.6520 0.0489 SEX DOSE GROUP F1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (0) 5 257.4 31.4 ORGAN WEIGHT (0) 5 0.9348 0.1123 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 5 0.3637 0.0252 5 0.4616 0.0441 GTrroo M5 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 317.3* 50.6 5 1.4785 0.2950 5 0.4640* 0.0242 5 0.7191 0.1033 F5 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 170.3* 14.1 5 0.7994* 0.0471 5 0.4710* 0.0323 5 0.4400 0.0132 TABLE 45 Summary of Organ Height Data Week 8 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PEHFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 6 TABLE INCLUDES: SEX=ALL;GROUP=l,5;WBEKS=ALL DEATH=2;SUBSET=ALL LIVER SEX DOSE GROUP H1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (9) ORGAN WEIGHT (9) 5 442.3 31.8 5 11.4244 1.6279 ORGAN-TOBODY WT (%) 5 2.5734 0.2217 ORGAN-TOBRAIN WT RATIO 5 5.2578 0.7478 SEX DOSE GROUP F1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (9) ORGAN WEIGHT (9) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 5 257.4 31.4 5 7.2921 0.7864 5 2.8388 0.1198 5 3.6049 0.3306 6 M5 F5 NUMBER IN GROUP: 55 5 5 NUMBER IN GROUP: 55 5 5 Z>i MEAN: 317.3* 21.6104* 6.8321* 10.5688* MEAN: 170.3* 11.7394* 6.9106* 6.4698* oN STANDARD DEV: 50.6 3.4376 0.5778 1.4711 STANDARD DEV: 14.1 0.7410 0.3939 0.4478 TABLE 45 Summary of Organ Weight Data Week 8 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 7 TABLE INCLUDBS: SEX=ALL ;GROUP=1,5;WEEKS=ALL DEATH=2;SUBSET=ALL LUNG SEX DOSE GROUP M1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) 5 442.3 31.8 ORGAN WEIGHT (g) 5 1.4802 0.1217 ORGAN-TOBODY WT (%) 5 0.3350 0.0213 ORGAN-TOBRAIN WT RATIO 5 0.6809 0.0501 SEX DOSE GROUP F1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) 5 257.4 31.4 ORGAN WEIGHT (g) ORGAN-TOBODY WT (%) ORGAN-TO BRAIN WT RATIO 5 1.2546 0.1756 5 0.4874 0.0348 5 0.6195 0.0726 M5 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 317.3* 50.6 5 1.2530 0.2690 5 0.3928* 0.0306 5 0.6091 0.0971 F5 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 170.3* 14.1 5 0.8541* 0.0609 5 0.5028 0.0340 5 0.4704 0.0313 COo H* TABLE 45 Summary of Organ Weight Data Week 8 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance G329-212 3M T-6316.1 PAGE: 8 TABLE INCLUDES: SEX=ALL ;GROUP=l,5;WEEKS=ALL DEATH=2 ;SUBSET=ALL LEFT OVARY SEX DOSE GROUP TERMINAL BODY WT (g) ORGAN WEIGHT (g) ORGAN-TOBODY WT () ORGAN-TOBRAIN WT RATIO SEX DOSE GROUP TERMINAL BODY WT (9) ORGAN WEIGHT (9) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO M1 F1 NUMBER IN GROUP: MEAN: STANDARD DEV: oO M5 `i Co NUMBER IN GROUP: MEAN: t o STANDARD DEV: to 5 442.3 31.8 5 317.3* 50. G NUMBER IN GROUP: MEAN: STANDARD DEV: F5 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 257.4 31.4 5 0.0663 0.0160 5 0.0255 0.0043 5 170.3* 14.1 5 0.0468* 0.0078 5 0.0276 0.0048 5 0.0326 0.0070 5 0.0258 0.0048 TABLE 45 Summary of Organ Height Data Heek 8 Sacrifice 104-HEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 9 TABLE INCLUDES: SEX=ALL;GROUP=l,5;WEEKS=ALL DEATH=2;SUBSET=ALL RIGHT OVARY SEX DOSE GROUP M1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY HT (g) 5 442.3 31.8 ORGAN WEIGHT (g) ORGAN-TOBODY HT {) ORGAN-TOBRAIN HT RATIO SEX DOSE GROUP F1 NUMBER IN GROUP MEAN STANDARD DEV TERMINAL BODY WT (3) ORGAN WEIGHT (3) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 5 257.4 31.4 5 0.0733 0.0184 5 0.0282 0.0044 5 0.0361 0.0081 co M5 CJ M NUMBER IN GROUP: MEAN: S 317.3* STANDARD DEV: 50.6 F5 NUMBER IN GROUP MEAN STANDARD DEV 5 170.3* 14.1 5 0.0527 0.0085 5 0.0311 0.0050 5 0.0291 0.0052 TABLE 45 Summary of O r g a n Height Data Week 8 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 10 TABLE INCLUDES: SEX=ALL;GROUP=l,5;WBEKS=ALL DEATH=2 ;SUBSET=ALL SPLEEN CO SEX DOSE GROUP M1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT <g> 5 442.3 31.8 ORGAN WEIGHT (g) 5 0.7326 0.1440 ORGAN-TOBODY WT (%) 5 0.1646 0.0248 ORGAN-TOBRAIN WT RATIO 5 0.3372 0.0666 SEX DOSE GROUP F1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) 5 257.4 31.4 ORGAN WEIGHT (g) 5 0.4972 0.0520 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 5 0.1942 0.0188 5 0.2454 0.0142 Co M 5 roW NUMBER IN GROUP: MEAN: STANDARD DEV: 5 317.3* 50.6 5 0.5901 0.1298 5 0.1852 0.0199 5 0.2869 0.0482 F5 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 170.3* 14.1 5 0.3599* 0.0407 5 0.2127 0.0312 5 0.1981' 0.0193 TABLE 45 Summary of Organ Weight Data Week 8 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANBSULFONAMIDO ETHANOL IN RATS Covance 6329-212 3H T-6316.1 PAGE: 11 TABLE INCLUDES: SEX=ALL;GROUP=1,5;WEEKS=ALL DEATH=2;SUBSET=ALL LEFT TESTIS SEX DOSE GROUP TERMINAL BODY WT (?) ORGAN WEIGHT (0) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO SEX DOSE GROUP TERMINAL BODY WT (g) ORGAN WEIGHT (g) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO M1 F1 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 442.3 31.8 5 1.4941 0.1390 5 0.3378 0.0185 5 0.6875 0.0615 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 257.4 31.4 N5 F5 C-* NUMBER IN GROUP: 5 5 5 5 NUMBER IN GROUP: 5 Cj MEAN: 317.3* 1.7529* 0.5580* 0.8581* MEAN: 170.3* STANDARD DEV: 50.6 0.1520 0.0501 0.0633 STANDARD DEV: 14.1 ro TABLE 45 Summary of Organ Weight Data Week 8 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 12 TABLE INCLUDES: SEX=ALL;GROUP=l,5;WEEKS=ALL DEATH=2;SUBSET=ALL RIGHT TESTIS SEX DOSE GROUP M1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) 5 442.3 31.8 ORGAN WEIGHT (g) 5 1.5159 0.1642 ORGAN-TOBODY WT (%) 5 0.3424 0.0221 ORGAN-TOBRAIN WT RATIO 5 0.6976 0.0746 SEX DOSE GROUP F1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) 5 257.4 31.4 ORGAN WEIGHT (g) ORGAN-TOBODY WT (%) ORGAN-TO BRAIN WT RATIO M5 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 317.3* 50.6 5 1.7399 0.1642 5 0.5535* 0.0492 5 0.8511* 0.0601 F5 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 170.3* 14.1 TABLE 45 Summary of Organ Weight Data Week 8 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 13 TABLE INCLUDES: SEX=ALL; GROUP=l,5;WEEKS=ALL DEATH=2;SUBSET=ALL LEFT THYROID/PAR SEX DOSE GROUP TERMINAL BODY WT (0) ORGAN WEIGHT (0) ORGAN-TOBODY WT (%) ORGAN-TO- BRAIN WT RATIO SEX DOSE GROUP TERMINAL BODY WT (0) ORGAN WEIGHT (0) ORGAN-TOBODY WT (%) ORGAN-TO BRAIN WT RATIO H1 F1 in 1 X NUMBER IN GROUP MEAN o STANDARD DEV o C-> C v NUMBER IN GROUP r o MEAN STANDARD DEV 5 442.3 31.8 4 0.0128 0.0026 4 0.0028 0.0006 5 317.3* 50.6 4 0.0093 0.0015 4 0.0031 0.0009 4 0.0059 0.0012 NUMBER IN GROUP: MEAN: STANDARD DEV: 4 0.0047 0.0012 F5 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 257.4 31.4 5 0.0083 0.0021 5 0.0032 0.0007 5 0.0041 0.0010 5 170.3* 14.1 4 0.0076 0.0022 4 0.0043 0.0013 4 0.0041 0.0013 TABLE 45 Summary of Organ Height Data Week 8 sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 14 TABLE INCLUDES: SEX=ALL;GROUP=l,5;WEEKS=ALL DEATH=2;SUBSET=ALL RIGHT THYROID/PR SEX DOSE GROUP TERMINAL BODY WT (g> ORGAN WEIGHT (g) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO SEX DOSE GROUP TERMINAL BODY WT (g> ORGAN WEIGHT tg> ORGAN-TOBODY WT (%> ORGAN-TO BRAIN WT RATIO M1 F1 NUMBER IN GROUP: o MEAN: STANDARD DEV: o e- CO M 5 w NUMBER IN GROUP: G3 MEAN: STANDARD DEV: 5 442.3 31.8 5 0.0113 0.0049 5 317.3* 50.6 5 0.0102 0.0033 5 0.0025 0.0010 5 0.0032 0.0009 5 0.0052 0.0022 5 0.0050 0.0015 NUMBER IN GROUP: MEAN: STANDARD DEV: F5 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 257.4 31.4 5 0.0074 0.0022 5 170.3* 14.1 5 0.0078 0.0024 5 0.0029 0.0008 5 0.0046 0.0016 5 0.0037 0.0011 5 0.0043 0.0014 TABLE 45 Summary of Organ Weight Data Week 8 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 15 TABLE INCLUDES: SEX=ALL;GROUP=l,5;WEEKS=ALL DEATH=2;SUBSET=ALL UTERUS SEX DOSE GROUP M1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) 5 442.3 31.8 ORGAN WEIGHT (g) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO SEX DOSE GROUP F1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (?) 5 257.4 31.4 ORGAN WEIGHT (9) 5 0.5992 0.1269 ORGAN-TOBODY WT <%) ORGAN-TOBRAIN WT RATIO 5 0.2334 0.0468 5 0.2958 0.0584 M5 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 317.3* 50.6 F5 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 170.3* 14.1 5 0.4823 0.1903 5 0.2821 0.1076 5 0.2633 0.0945 TABLE 46 Summary of Organ Height Data Week 14 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 1 TABLE INCLUDES: SEX=ALL;GROUP=l,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL LEFT ADRENAL SEX DOSE GROUP M1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (ff) 5 535.9 54.5 ORGAN WEIGHT (9) 5 0.0373 0.0080 ORGAN-TOBODY WT (%) 5 0.0069 0.0008 ORGAN-TOBRAIN WT RATIO 5 0.0168 0.0035 SEX DOSE GROUP F1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (ff) 5 291.3 46.9 ORGAN WEIGHT (9) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 5 0.0336 0.0052 5 0.0119 0.0032 5 0.0169 0.0030 n to M2 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 531.5 24.6 5 0.0277 0.0061 5 0.0052 0.0013 5 0.0127 0.0031 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 255.2 30.7 5 0.0360 0.0055 5 0.0142 0.0024 5 0.0185 0.0025 M3 NUMBER IN GROUP MEAN STANDARD DEV 5 539.3 70.5 5 0.0295 0.0022 5 0.0055 0.0007 5 0.0129 0.0010 F3 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 264.2 31.1 5 0.0369 0.0077 5 0.0141 0.0030 5 0.0186 0.0035 TABLE 46 Summary of Organ Weight Data Week 14 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 2 TABLE INCLUDES: SEX=ALL;GROUP=l,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL LEFT ADRENAL SEX DOSE GROUP M4 TERMINAL BODY wr (?) ORGAN WEIGHT <g> ORGAN-TOBODY WT (%) ORGAN-TO- BRAIN WT RATIO SEX DOSE GROUP F4 TERMINAL BODY WT <g> ORGAN WEIGHT (g) ORGAN-TOBODY WT (%) ORGAN-TO- BRAIN WT RATIO NUMBER IN GROUP: MEAN: STANDARD DEV: 5 473.3 39.1 5 0.0303 0.0034 5 0.0065 0.0011 5 0.0139 0.0019 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 236.9 21.2 5 0.0322 0.0049 5 0.0137 0.0027 5 0.0173 0.0036 TABLE 46 Summary of Organ Height Data Week 14 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-BTHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 3 TABLE INCLUDES: SEX=ALL;GROUP=l,2,3,4;WBBKS-ALL DEATH=3 ;SUBSET=ALL RIGHT ADRENAL SEX DOSE GROUP TERMINAL BODY WT (0) ORGAN WEIGHT (0) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO SEX DOSE GROUP TERMINAL BODY WT (0) ORGAN WEIGHT (0) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO M1 F1 NUMBER IN GROUP: 55 5 5 NUMBER IN GROUP o MEAN: 535.9 0.0353 0.0065 0.0159 MEAN o STANDARD DEV: 54.5 0.0076 0.0009 0.0033 STANDARD DEV CJ CO M 2 F2 C J NUMBER IN GROUP: 55 5 5 NUMBER IN GROUP to MEAN: 531.5 0.0256* 0.0048* 0.0118 MEAN STANDARD DEV: 24.6 0.0043 0.0009 0.0023 STANDARD DEV 5 291.3 46.9 5 0.0337 0.0071 5 0.0119 0.0034 5 0.0171 0.0042 5 255.2 30.7 5 0.0336 0.0052 5 0.0132 0.0016 5 0.0173 0.0024 M3 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 539.3 70.5 5 0.0273 0.0034 5 0.0051* 0.0004 5 0.0119 0.0012 F3 NUMBER IN GROUP MEAN STANDARD DEV 5 264.2 31.1 5 0.0345 0.0059 5 0.0131 0.0019 5 0.0174 0.0025 TABLE 46 Summary of Organ Height Data Week 14 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 4 TABLE INCLUDES: SEX=ALL;GROUP=l,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL RIGHT ADRENAL SEX DOSE GROUP M4 TERMINAL BODY WT (g) ORGAN WEIGHT <ff> ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO SEX DOSE GROUP F4 TERMINAL BODY WT (g> ORGAN WEIGHT (g) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO rrcroo NUMBER IN GROUP: MEAN: STANDARD DEV: 5 473.3 39.1 S 0.0286 0.0030 5 0.0061 0.0009 5 0.0131 0.0018 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 236.9 21.2 5 0.0307 0.0036 5 0.0131 0.0023 5 0.0165 0.0028 TABLE 46 Summary of Organ Waight Data Week 14 sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 5 TABLE INCLUDES: SEX=ALL;GROUP=l,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL BRAIN SEX DOSE GROUP TERMINAL (a)BODY WT ORGAN <g)WEIGHT ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO SEX DOSE GROUP TERMINAL (g)BODY WT ORGAN <g)WEIGHT ORGAN-TOBODY WT (%) ORGAN-TO BRAIN WT RATIO M1 F1 NUMBER IN GROUP: 55 5 5 NUMBER IN GROUP: 55 5 5 o MEAN: 535.9 2.2215 0.4182 1.0000 MEAN: 291.3 1.9905 0.6962 1.0000 o STANDARD DEV: 54.5 0.0445 0.0452 0.0000 STANDARD DEV: 46.9 0.0791 0.1058 0.0000 Co M2 F2 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 531.5 24.6 5 2.1920 0.0960 5 0.4126 0.0123 5 1.0000 0.0000 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 255.2 30.7 5 1.9423 0.1026 5 0.7665 0.0587 5 1.0000 0.0000 334 M3 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 539.3 70.5 5 2.2818 0.1054 5 0.4273 0.0430 5 1.0000 0.0000 F3 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 264.2 31.1 5 1.9846 0.1121 5 0.7566 0.0628 5 1.0000 0.0000 TABLE 46 Summary of Organ Weight Data Week 14 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-BTHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 6 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL BRAIN SEX DOSE GROUP M4 TERMINAL BODY WT (g) ORGAN WEIGHT la) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO SEX DOSE GROUP F4 TERMINAL BODY WT (g) ORGAN WEIGHT (g) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO NUMBER IN GROUP: 55 5 5 NUMBER IN GROUP: 5 5 5 5 MEAN: 473.3 2.1861 0.4642 1.0000 MEAN: 236.9 1.8793 0.7961 1.0000 STANDARD DEV: 39.1 0.0723 0.0397 0.0000 STANDARD DEV: 21.2 0.1322 0.0609 0.0000 e-3 co CO cn co cCoo CO & TABLE 46 Summary of Organ Height Data Week 14 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WIT NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 7 TABLE INCLUDES: SEX=ALL;GROUP=l,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL LEFT KIDNEY SEX DOSE GROUP M1 NUMBER IN GROUP MEAN STANDARD DEV TERMINAL BODY WT (g) ORGAN WEIGHT (g) 5 535.9 54.5 5 1.9711 0.1974 ORGAN-TOBODY WT (%) 5 0.3686 0.0233 ORGAN-TOBRAIN WT RATIO 5 0.8874 0.0880 SEX DOSE GROUP F1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT <g> 5 291.3 46.9 ORGAN WEIGHT (g) 5 1.1328 0.1492 ORGAN-TOBODY WT (%) ORGAN-TO BRAIN WT RATIO 5 0.3911 0.0283 5 0.5688 0.0667 M2 NUMBER IN GROUP MEAN STANDARD DEV 5 531.5 24.6 5 1.8955 0.1575 5 0.3570 0.0307 5 0.8661 0.0818 F2 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 255.2 30.7 5 0.9311 0.1262 5 0.3658 0.0386 5 0.4782 0.0473 M3 NUMBER IN GROUP MEAN STANDARD DEV 5 539.3 70.5 5 2.0805 0.2924 5 0.3863 0.0302 5 0.9111 0.1154 F3 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 264.2 31.1 5 1.1345 0.1316 5 0.4307 0.0362 5 0.5718 0.0615 TABLE 46 Summary of Organ Height Data Week 14 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3H T-6316.1 PAGE: 8 TABLE INCLUDES: SEX=ALL;GROUP=l,2,3,4:WEEKS=ALL DEATH=3;SUBSET=ALL LEFT KIDNEY SEX DOSE GROUP M4 TERMINAL BODY WT (?) ORGAN WEIGHT (?) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO SEX DOSE GROUP F4 TERMINAL BODY WT (?) ORGAN WEIGHT (?) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO NUMBER IN GROUP: MEAN: STANDARD DEV: 5 473.3 39.1 5 2.1488 0.2660 5 0.4535* 0.0313 5 0.9817 0.1017 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 236.9 21.2 5 0.9648 0.1536 5 0.4053 0.0302 5 0.5124 0.0648 oo Cj CO co QO TABLE 46 Summary of Organ Weight Data Week 14 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1*) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 9 TABLE INCLUDES: SEX=ALL;GR0UP=1, 2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL RIGHT KIDNEY SEX DOSE GROUP M1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) 5 535.9 54.5 ORGAN WEIGHT (g> 5 2.0585 0.2090 ORGAN-TOBODY WT (%) 5 0.3846 0.0203 ORGAN-TOBRAIN WT RATIO 5 0.9265 0.0904 SEX DOSE GROUP F1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) 5 291.3 46.9 ORGAN WEIGHT (g) 5 1.1236 0.1700 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 5 0.3874 0.0341 5 0.5644 0.0797 M2 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 531.5 24.6 5 1.9405 0.1411 5 0.3656 0.0297 5 0.8873 0.0831 F2 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 255.2 30.7 5 0.9328 0.1505 5 0.3652 0.0354 5 0.4787 0.0596 M3 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 539.3 70.5 5 2.0643 0.2531 5 0.3835 0.0209 5 0.9041 0.0972 F3 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 264.2 31.1 5 1.1634 0.1471 5 0.4418 0.0451 5 0.5865 0.0712 TABLE 46 Summary of Organ Height Data Week 14 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 10 TABLE INCLUDES: SEX=ALL;GROUP=l ,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL RIGHT KIDNEY SEX DOSE GROUP H4 TERMINAL BODY WT <g> ORGAN WEIGHT (g) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO SEX DOSE GROUP F4 TERMINAL BODY WT (a) ORGAN WEIGHT (g) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO NUMBER IN GROUP: MEAN: STANDARD DEV: 5 473.3 39.1 5 2.1724 0.2812 5 0.4581* 0.0303 5 0.9929 0.1136 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 236.9 21.2 5 0.9912 0.1534 5 0.4166 0.0305 5 0.5264 0.0638 TABLE 46 Summary of Organ Weight Data Week 14 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 11 TABLE INCLUDES: SEX=ALL;GROUP=l,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL LIVER SEX DOSE GROUP TERMINAL BODY WT (g) ORGAN WEIGHT (g) ORGAN-TOBODY WT () ORGAN-TOBRAIN WT RATIO SEX DOSE GROUP TERMINAL BODY WT (g) ORGAN WEIGHT (a) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO M1 F1 NUMBER IN GROUP: 55 5 5 NUMBER IN GROUP: 55 5 5 MEAN: 535.9 15.9996 2.9783 7.2008 MEAN: 291.3 8.7487 3.0096 4.4018 o STANDARD DEV: 54.5 2.1505 0.1425 0.9432 STANDARD DEV: 46.9 1.2794 0.1220 0.6612 Cm Cm M 2 F2 NUMBER IN GROUP: MEAN: 5 531.5 5 15.6102 5 2.9345 5 7.1245 NUMBER IN GROUP: MEAN: 5 255.2 5 7.4501 5 2.9233 5 3.8304 STANDARD DEV: 24.6 1.7249 0.2531 0.7384 STANDARD DEV: 30.7 1.0845 0.2753 0.4607 M3 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 539.3 70.5 5 18.0657 1.7387 5 3.3764 0.3717 5 7.9217 0.7535 F3 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 264.2 31.1 5 8.8944 1.3628 5 3.3658 0.3182 5 4.4714 0.5448 TABLE 46 Summary of Organ Height Data Week 14 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 12 TABLE INCLUDES: SEX=ALL;GROUP=l,2,3,4;WEEKS=ALL DEATH=3 ;SUBSET=ALL LIVER SEX DOSE GROUP M4 TERMINAL BODY WT (9) ORGAN WEIGHT (9) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO SEX DOSE GROUP F4 TERMINAL BODY WT (3) ORGAN (a)WEIGHT ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO Tfrrroo NUMBER IN GROUP: MEAN: STANDARD DEV: 5 473.3 39.1 5 24.2906* 3.5145 5 5.1173* 0.3964 5 11.1093* 1.5191 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 236.9 21.2 5 9.7445 1.1880 5 4.1155* 0.3584 5 5.1846 0.5040 TABLE 46 Summary of Organ Weight Data Week 14 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 13 TABLE INCLUDES: SEX=ALL;GR0UP=1,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL LUNG SEX DOSE GROUP M1 NUMBER IN GROUP MEAN STANDARD DEV TERMINAL BODY WT (g) 5 535.9 54.5 ORGAN WEIGHT (g) 5 1.8736 0.1592 ORGAN-TOBODY WT (%) 5 0.3510 0.0280 ORGAN-TOBRAIN WT RATIO 5 0.8428 0.0609 SEX DOSE GROUP F1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) 5 291.3 46.9 ORGAN WEIGHT (g) ORGAN-TOBODY WT () ORGAN-TOBRAIN WT RATIO 5 1.2020 0.1063 5 0.4182 0.0552 5 0.6048 0.0590 M2 NUMBER IN GROUP MEAN STANDARD DEV 5 531.5 24.6 5 1.7104 0.0972 5 0.3226 0.0267 5 0.7811 0.0493 F2 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 255.2 30.7 5 1.1997 0.1562 5 0.4698 0.0101 5 0.6163 0.0571 M3 NUMBER IN GROUP MEAN STANDARD DEV 5 539.3 70.5 5 1.9907 0.2684 5 0.3701 0.0333 5 0.8707 0.0899 F3 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 264.2 31.1 5 1.1830 0.0601 5 0.4513 0.0396 5 0.5968 0.0305 TABLE 46 Summary of organ Weight Data Week 14 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 14 TABLE INCLUDES: SEX=ALL;GROUPsl,2,3,4; WEEKS=ALL DEATH=3;SUBSET=ALL SEX DOSE GROUP M4 TERMINAL BODY WT (g) ORGAN WEIGHT (g> LUNG ORGAN-TOBODY WT {%) ORGAN-TO BRAIN WT RATIO SEX DOSE GROUP F4 TERMINAL BODY WT <g) ORGAN WEIGHT (g) ORGAN-TOBODY WT (%) ORGAN-TO BRAIN WT RATIO NUMBER IN GROUP: MEAN: STANDARD DEV: 5 473.3 39.1 5 1.6290 0.1108 5 0.3467 0.0448 5 0.7450 0.0411 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 236.9 21.2 5 1.0859 0.0776 5 0.4601 0.0363 5 0.5784 0.0303 c C CO CO TABLE 46 Summary of organ Weight Data Week 14 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 15 TABLE INCLUDES: SEX=ALL;GROUPAI,2,3,4;WEEKS=ALL DEATH=3 ;SUBSET=ALL LEFT OVARY SEX DOSE GROUP M1 NUMBER IN GROUP MEAN STANDARD DEV TERMINAL BODY WT (g) 5 535.9 54.5 ORGAN WEIGHT (a) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO SEX DOSE GROUP F1 NUMBER IN GROUP MEAN STANDARD DEV TERMINAL (g)BODY WT ORGAN (g)WEIGHT ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 5 291.3 46.9 5 0.0742 0.0125 5 0.0263 0.0072 5 0.0374 0.0067 M2 NUMBER IN GROUP 5 o MEAN 531.5 6 STANDARD DEV 24.6 r-, M3 > NUMBER IN GROUP 5 MEAN 539.3 STANDARD DEV 70.5 F2 NUMBER IN GROUP MEAN STANDARD DEV F3 NUMBER IN GROUP MEAN STANDARD DEV 5 255.2 30.7 5 0.0523 0.0097 5 0.0206 0.0034 5 0.0269 0.0043 5 264.2 31.1 5 0.0608 0.0201 5 0.0230 0.0070 5 0.0304 0.0093 TABLE 46 Summary of Organ Weight Data Week 14 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 16 TABLE INCLUDES: SEX=ALL;GROUP=l,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL LEFT OVARY SEX DOSE GROUP M4 TERMINAL BODY WT (ff) ORGAN WEIGHT (0) ORGAN-TOBODY WT () ORGAN-TOBRAIN WT RATIO SEX DOSE GROUP F4 TERMINAL BODY WT (0) ORGAN WEIGHT (0> ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO NUMBER IN GROUP: MEAN: STANDARD DEV: 5 473.3 39.1 e Co Cw P* cn NUMBER IN GROUP: MEAN: STANDARD DEV: 5 236.9 21.2 5 0.0514 0.0017 5 0.0218 0.0016 5 0.0275 0.0022 TABLE 46 Summary of Organ Height Data Week 14 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 17 TABLE INCLUDES: SEX=ALL ;GROUPAI ,2,3,4; WEEKS=ALL DEATH=3;SUBSET=ALL RIGHT OVARY SEX DOSE GROUP M1 NUMBER IN GROUP MEAN STANDARD DEV TERMINAL BODY WT <g> 5 535.9 54.5 ORGAN WEIGHT (g) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO SEX DOSE GROUP F1 NUMBER IN GROUP MEAN STANDARD DEV TERMINAL BODY WT (g) ORGAN WEIGHT (g) ORCAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 5 291.3 46.9 5 0.0596 0.0166 5 0.0208 0.0061 5 0.0301 0.0090 3 M2 NUMBER IN GROUP MEAN STANDARD DEV 5 531.5 24.6 F2 NUMBER IN GROUP MEAN STANDARD DEV 5 255.2 30.7 5 0.0518 0.0140 5 0.0201 0.0047 5 0.0264 0.0063 M3 NUMBER IN GROUP MEAN STANDARD DEV 5 539.3 70.5 F3 NUMBER IN GROUP MEAN STANDARD DEV 5 264.2 31.1 5 0.0623 0.0128 S 0.0236 0.0040 5 0.0312 0.0054 TABLE 46 Summary of Organ Haight Data Week 14 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covanee 6329-212 3M T-6316.1 PAGE: 18 TABLE INCLUDES: SEX=ALL;GROUP=l,2,3,4;WEEKSsALL DEATH=3;SUBSET=ALL RIGHT OVARY SEX DOSE GROUP M4 TERMINAL BODY WT <g> ORGAN WBIGHT (g) ORGAN-TOBODY WT (%) ORGAN-TO- BRAIN WT RATIO SEX DOSE GROUP F4 TERMINAL BODY WT <g) ORGAN WEIGHT (g) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO NUMBER IN GROUP: MEAN: STANDARD DEV: C Cv 5 473.3 39.1 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 236.9 21.2 5 0.0541 0.0054 5 0.0229 0.0026 5 0.0289 0.0036 TABLE 46 Summary of Organ Height Data Week 14 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 19 TABLE INCLUDES: SEX=ALL;GROUP=l,2,3,4; WEEKS=ALL DEATH=3;SUBSET=ALL SPLEEN SEX DOSE GROUP M1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) 5 535.9 54.5 ORGAN WEIGHT (g) 5 0.7252 0.1659 ORGAN-TOBODY WT (%) 5 0.1345 0.0257 ORGAN-TOBRAIN WT RATIO 5 0.3266 0.0755 SEX DOSE GROUP F1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) 5 291.3 46.9 ORGAN WEIGHT (g) 5 0.4690 0.0560 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 5 0.1622 0.0152 5 0.2360 0.0293 M2 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 531.5 24.6 5 0.8193 0.0229 5 0.1544 0.0079 5 0.3743 0.0186 F2 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 255.2 30.7 5 0.4603 0.0829 5 0.1793 0.0145 5 0.2358 0.0329 M3 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 539.3 70.5 5 0.7152 0.1209 5 0.1328 0.0166 5 0.3135 0.0524 F3 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 264.2 31.1 5 0.4512 0.0531 5 0.1711 0.0124 5 0.2272 0.0226 TABLE 46 Summary of Organ Weight Data Week 14 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 20 TABLE INCLUDES: SEX=ALL;GROUP=1,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL SPLEEN SEX DOSE GROUP M4 TERMINAL BODY WT (a) ORGAN WEIGHT (0) ORGAN-TOBODY WT (%) ORGAN-TO BRAIN WT RATIO SEX DOSE GROUP F4 TERMINAL BODY WT (0) ORGAN WEIGHT (0) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO NUMBER IN GROUP: MEAN: STANDARD DEV: 5 473.3 39.1 5 0.6639 0.0293 5 0.1408 0.0101 5 0.3037 0.0066 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 236.9 21.2 5 0.4064 0.0722 5 0.1730 0.0356 5 0.2170 0.0408 TABLE 46 Summary of Organ Haight Data Week 14 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 21 TABLE INCLUDES: SEX=ALL;GROUP=l,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL LEFT TESTIS SEX DOSE GROUP M1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (G) 5 535.9 54.5 ORGAN WEIGHT (a) 5 1.6975 0.1684 ORGAN-TOBODY WT (%) 5 0.3204 0.0570 ORGAN-TOBRAIN WT RATIO 5 0.7645 0.0789 SEX DOSE GROUP F1 NUMBER IN GROUP MEAN STANDARD DEV TERMINAL BODY WT (g) ORGAN WEIGHT (g) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 5 291.3 46.9 M2 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 531.5 24.6 5 1.7368 0.0912 5 0.3270 0.0161 5 0.7924 0.0251 P2 NUMBER IN GROUP MEAN STANDARD DEV 5 255.2 30.7 ti 3 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 539.3 70.5 5 1.6528 0.0711 5 0.3100 0.03 67 5 0.7254 0.0433 F3 NUMBER IN GROUP MEAN STANDARD DEV 5 264.2 31.1 TABLE 46 Summary of Organ Weight Data Week 14 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covane 6329-212 3H T-6316.1 PAGE: 22 TABLE INCLUDES: SEX-ALL:GROUP=l,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL LEFT TESTIS SEX DOSE GROUP M4 TERMINAL BODY WT <S) ORGAN WEIGHT <g> ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO SEX DOSE GROUP F4 TERMINAL BODY WT (g> ORGAN WEIGHT Iff) ORGAN-TOBODY WT 1%) ORGAN-TOBRAIN WT RATIO NUMBER IN GROUP: MEAN: STANDARD DEV: 5 473.3 39.1 5 1.7151 0.1400 5 0.3629 0.0209 5 0.7841 0.0500 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 236.9 21.2 TABLE 46 Summary of Organ Height Data Week 14 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 23 TABLE INCLUDES: SEX=ALL;GROUP=l,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL RIGHT TESTIS SEX DOSE GROUP TERMINAL BODY WT (9) ORGAN WEIGHT tg) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO SEX DOSE GROUP TERMINAL BODY WT (g) ORGAN WEIGHT (g) ORGAN-TOBODY WT <%> ORGAN-TOBRAIN WT RATIO M1 F1 NUMBER IN GROUP MEAN Co STANDARD DEV Co in M 2 NUMBER IN GROUP MEAN STANDARD DEV 5 535.9 54.5 5 1.7042 0.1307 5 531.5 24.6 5 1.7733 0.1140 5 0.3211 0.0473 5 0.3339 0.0204 5 0.7677 0.0648 NUMBER IN GROUP MEAN STANDARD DEV 5 0.8087 0.0315 F2 NUMBER IN GROUP: MEAN STANDARD DEV 5 291.3 46.9 5 255.2 30.7 M3 NUMBER IN GROUP MEAN STANDARD DEV 5 539.3 70.5 5 1.6684 0.0976 5 0.3131 0.0402 5 0.7324 0.0561 F3 NUMBER IN GROUP MEAN STANDARD DEV 5 264.2 31.1 TABLE 46 Summary of organ Weight Data Week 14 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 24 TABLE INCLUDES: SEX=ALL;GROUP=l.2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL RIGHT TESTIS SEX DOSE GROUP M4 TERMINAL BODY WT (a) ORGAN WEIGHT (g) ORGAN-TOBODY WT () ORGAN-TOBRAIN WT RATIO SEX DOSE GROUP F4 TERMINAL BODY WT (g) ORGAN WEIGHT (a) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO NUMBER IN GROUP: MEAN: STANDARD DEV: 5 473.3 39.1 5 1.7278 0.1665 5 0.3653 0.0253 5 0.7902 0.0681 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 236.9 21.2 TABLE 46 Summary of Organ Weight Data Week 14 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANBSULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 25 TABLE INCLUDES: SEX=ALL;GROUP=l,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL LEFT THYROID/PAR SEX DOSE GROUP TERMINAL BODY WT iff) ORGAN WEIGHT (g) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO SEX DOSE GROUP TERMINAL BODY WT (g) ORGAN WEIGHT (g) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO M1 F1 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 53S.9 54.5 5 0.0125 0.0029 5 0.0023 0.0005 5 0.0056 0.0012 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 291.3 46.9 5 0.0105 0.0040 5 0.0036 0.0013 5 0.0053 0.0022 C c- M 2 F2 Co NUMBER IN GROUP 55 5 5 NUMBER IN GROUP: 55 5 5 cn MEAN 531.5 0.0114 0.0021 0.0052 MEAN: 255.2 0.0076 0.0029 0.0039 & STANDARD DEV 24.6 0.0022 0.0004 0.0010 STANDARD DEV: 30.7 0.0025 0.0007 0.0012 M3 NUMBER IN GROUP MEAN STANDARD DEV 5 539.3 70.5 5 0.0132 0.0023 5 0.0025 0.0006 5 0.0058 0.0012 F3 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 264.2 31.1 5 0.0093 0.0025 5 0.0035 0.0008 5 0.0047 0.0012 TABLE 46 Summary of Organ Weight Data Week 14 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=l,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL LEFT THYROID/PAR SEX DOSE GROUP M4 TERMINAL BODY WT (g) ORGAN WEIGHT (g) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO SEX DOSE GROUP F4 TERMINAL BODY WT (0) ORGAN WEIGHT (g) Covance 6329-212 3M T-6316.1 PAGE: 26 ORGAN-TOBODY WT (%) ORGAN-TO- BRAIN WT RATIO e NUMBER IN GROUP: 5 5 5 5 NUMBER IN GROUP: 55 5 5 r> MEAN: 473.3 0.0144 0.0031 0.0066 MEAN: 236.9 0.0092 0.0039 0.0049 Co STANDARD DEV: 39.1 0.0008 0.0003 0.0005 STANDARD DEV: 21.2 0.0017 0.0005 0.0007 C/T cn oc* O c/i TABLE 46 Summary of Organ Height Data Week 14 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covanee 6329-212 3M T-6316.1 PAGE: 27 TABLE INCLUDES: SEX=ALL;GR0UP=1,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL RIGHT THYROID/PR SEX DOSE GROUP H1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT <g) 5 535.9 54.5 ORGAN WEIGHT (g) 5 0.0120 0.0021 ORGAN-TOBODY WT (%) 5 0.0022 0.0003 ORGAN-TOBRAIN WT RATIO 5 0.0054 0.0009 SEX DOSE GROUP F1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) 5 291.3 46.9 ORGAN WEIGHT (g) 5 0.0080 0.0026 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 5 0.0029 0.0011 5 0.0041 0.0014 H2 NUMBER IN GROUP MEAN STANDARD DEV 5 531.5 24.6 5 0.0128 0.0041 5 0.0024 0.0008 5 0.0058 0.0017 F2 NUMBER IN GROUP MEAN STANDARD DEV 5 255.2 30.7 5 0.0093 0.0033 5 0.0037 0.0014 5 0.0048 0.0017 M3 NUMBER IN GROUP MEAN STANDARD DEV 5 539.3 70.5 5 0.0138 0.0019 5 0.0026 0.0006 5 0.0061 0.0010 F3 NUMBER IN GROUP MEAN STANDARD DEV 5 264.2 31.1 5 0.0119 0.0042 5 0.0045 0.0013 5 0.0059 0.0019 TABLE 46 Summary of Organ Weight Data Week 14 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3H T-6316.1 PAGE: 28 TABLE INCLUDES: SEX=ALL;GROUP=l ,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL RIGHT THYROID/PR SEX DOSE GROUP H4 TERMINAL BODY WT (g) ORGAN WEIGHT (g> ORGAN-TOBODY WT <%> ORGAN-TOBRAIN WT RATIO SEX DOSE GROUP F4 TERMINAL BODY WT is) ORGAN WEIGHT (g) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO NUMBER IN GROUP: MEAN: 5 473.3 5 0.0125 5 0.0026 5 0.0057 NUMBER IN GROUP: MEAN: 5 236.9 5 0.0092 5 0.0039 5 0.0048 Cj STANDARD DEV: 39.1 0.0014 0.0002 0.0006 STANDARD DEV: 21.2 0.0021 0.0009 0.0008 C4 01 -J TABLE 46 Summary of Organ Weight Data Week 14 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=l ,2,3,4;WEEKS=ALL DEATH=3 ;SUBSET=ALL UTERUS Covance 6329-212 3M T-6316.1 PAGE: 29 SEX DOSE GROUP M1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT <g) ORGAN WEIGHT (g) 5 535.9 54.5 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO SEX DOSE GROUP F1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (0) 5 291.3 46.9 ORGAN WEIGHT (0) 5 0.6337 0.1022 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 5 0.2209 0.0448 5 0.3197 0.0613 M2 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 531.5 24.6 F2 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 255.2 30.7 5 0.5244 0.0677 5 0.2058 0.0151 5 0.2695 0.0269 M3 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 539.3 70.5 F3 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 264.2 31.1 5 0.6754 0.1666 5 0.2547 0.0519 5 0.3404 0.0847 TABLE 46 Summary of Organ Weight Data Week 14 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 30 TABLE INCLUDES: SEX=ALL;GROUP=l,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL UTERUS SEX DOSE GROUP TERMINAL BODY WT (0) ORGAN WEIGHT (0) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO SEX DOSE GROUP TERMINAL BODY WT (0) ORGAN WEIGHT (0) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO M4 <> Co NUMBER IN GROUP: MEAN: 5 473.3 ti STANDARD DEV: 39.1 C/T F4 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 236.9 21.2 5 0.5414 0.0477 5 0.2293 0.0207 5 0.2898 0.0368 TABLE 47 summary of organ Haight Data Week 14 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL ;GROUP=8,9; WEEKS=ALL DEATH=2 ;SUBSET=T LIVER Covance 6329-228 3M T-6316.1 PAGE: 1 SEX DOSE GROUP M8 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (0) ORGAN WEIGHT (0) S 501.8 59.4 5 14.4950 2.0800 ORGAN-TOBODY WT (%) 5 2.8829 0.1069 ORGAN-TOBRAIN WT RATIO SEX DOSE GROUP F8 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (0) 5 270.7 16.9 ORGAN WEIGHT (0) 5 8.0939 0.4382 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT PATIO 5 3.0001 0.2600 M9 C- Cw NUMBER IN GROUP: 54 4 MEAN: STANDARD DEV: 525.2 47.7 16.6952 2.7892 3.1146 0.2426 F9 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 254.9 23.0 5 7.5958 0.1717 5 2.9983 0.2565 TABLE 48 Summary of Organ Height Data Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 1 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKSsALL DEATH=4;SUBSET=ALL LEFT ADRENAL SEX DOSE GROUP TERMINAL BODY WT (0) ORGAN HBIGHT (0) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO SEX DOSE GROUP TERMINAL BODY WT (0) ORGAN WEIGHT (0) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO M1 F1 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 722.2 58.0 5 0.0350 0.0031 5 0.0049 0.0005 5 0.0147 0.0016 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 406.9 32.2 5 0.0445 0.0140 5 0.0112 0.0043 5 0.0202 0.0066 r- M4 F4 - NUMBER IN GROUP: 10 10 10 10 NUMBER IN GROUP: 10 10 10 10 w MEAN: 639.0* 0.0335 0.0053 0.0141 MEAN: 306.3* 0.0375 0.0125 0.0174 STANDARD DEV: 71.5 0.0057 0.0011 0.0025 STANDARD DEV: 49.7 0.0061 0.0030 0.0035 jil TABLE 48 Summary of Organ Height Data Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covane 6329-212 3M T-6316.1 PAGE: 2 TABLE INCLUDES: SEX=ALL;GROUP-ALL;WEEKS=ALL DEATH=4 ;SUBSET=ALL RIGHT ADRENAL SEX DOSE GROUP TERMINAL BODY WT <g> ORGAN HEIGHT (g) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO SEX DOSE GROUP TERMINAL BODY WT (g) ORGAN WEIGHT (g) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO CO. M1 F1 Cw NUMBER IN GROUP: 55 5 5 NUMBER IN GROUP: 55 5 5 Cv MEAN: 722.2 0.0316 0.0044 0.0132 MEAN: 406.9 0.0409 0.0103 0.0185 STANDARD DEV: 58.0 0.0049 0.0006 0.0016 STANDARD DEV: 32.2 0.0146 0.0044 0.0067 to M4 F4 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 639.0* 71.5 10 0.0324 0.0090 10 0.0052 0.0016 10 0.0137 0.0039 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 306.3* 49.7 10 0.0347 0.0041 10 0.0117 0.0025 10 0.0161 0.0024 TABLE 48 Summary of Organ Weight Data Week S3 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 3 TABLE INCLUDES: SEX=ALL ;GROUP=ALL ;WEEKS=ALL DEATH=4 ;SUBSET=ALL BRAIN SEX DOSE GROUP TERMINAL BODY WT (g> ORGAN WEIGHT (g) ORGAN-TOBODY WT (*) ORGAN-TOBRAIN WT RATIO SEX DOSE GROUP TERMINAL BODY WT <g) ORGAN WEIGHT (g) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO O M1 F1 O C - NUMBER IN GROUP: 55 5 5 NUMBER IN GROUP: 55 5 5 Co CO MEAN: STANDARD DEV: 722.2 58.0 2.3837 0.1629 0.3307 0.0180 1.0000 0.0000 MEAN: STANDARD DEV: 406.9 32.2 2.2100 0.0852 0.5452 0.0395 1.0000 0.0000 CO M4 F4 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 639.0* 71.5 10 2.3804 0.1067 10 0.3753* 0.0300 10 1.0000 0.0000 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 306.3* 49.7 10 2.1689 0.1318 10 0.7201* 0.0873 10 1.0000 0.0000 TABLE 48 Summary of Organ Weight Data Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 4 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=4;SUBSET=ALL LEFT KIDNEY m p ? oo SEX DOSE GROUP M1 NUMBER IN GROUP MEAN STANDARD DEV TERMINAL BODY WT (0) 5 722.2 58.0 ORGAN WEIGHT (0) 5 2.3249 0.1910 ORGAN-TOBODY WT (%) 5 0.3224 0.0201 ORGAN-TOBRAIN WT RATIO 5 0.9749 0.0261 SEX DOSE GROUP F1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (0) 5 406.9 32.2 ORGAN WEIGHT (0) 5 1.3605 0.1412 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 5 0.3375 0.0587 5 0.6164 0.0694 M4 NUMBER IN GROUP MEAN STANDARD DEV 10 639.0* 71.5 10 2.4578 0.3355 10 0.3856* 0.0421 10 1.0314 0.1225 F4 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 306.3* 49.7 10 1.4508 0.2497 10 0.4751* 0.0490 10 0.6686 0.1064 TABLE 48 Summary of Organ Weight Data Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 5 TABLE INCLUDES: SEX=ALL;GROUP=ALL ;WEEKS=ALL DEATH=4;SUBSET=ALL RIGHT KIDNEY SEX DOSE GROUP TERMINAL BODY WT (a) ORGAN WEIGHT (a) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO SEX DOSE GROUP TERMINAL BODY WT (a) ORGAN WEIGHT (a) ORGAN-TOBODY WT (%) ORGAN-TO BRAIN WT RATIO M1 F1 Co NUMBER IN GROUP 55 5 5 NUMBER IN GROUP: 55 5 5 Cj MEAN STANDARD DEV 722.2 58.0 2.3397 0.1416 0.3247 0.0166 0.9821 0.0202 MEAN: STANDARD DEV: 406.9 32.2 1.4036 0.1605 0.3486 0.0654 0.6372 0.0898 05 C/ M 4 F4 NUMBER IN GROUP MEAN STANDARD DEV 10 639.0* 71.5 10 2.4429 0.3646 10 0.3827* 0.0415 10 1.0246 0.1316 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 306.3* 49.7 10 1.5208 0.2577 10 0.4992* 0.0611 10 0.7013 0.1130 TABLE 48 Summary of Organ Weight Data Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE 198.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 6 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=4 ;SUBSET=ALL LIVER SEX DOSE GROUP M1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (0) ORGAN WEIGHT (0) 5 722.2 58.0 5 20.8522 1.6996 ORGAN-TOBODY WT 1%) 5 2.8910 0.1779 ORGAN-TOBRAIN WT RATIO 5 8.7696 0.8214 SEX DOSE GROUP F1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (0) ORGAN WEIGHT (0) ORGAN-TOBODY WT 1%) ORGAN-TOBRAIN WT RATIO 5 406.9 32.2 5 11.1366 0.5964 5 2.7565 0.3325 5 5.0503 0.4241 M4 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 639.0* 71.5 10 30.8941* 5.4082 10 4.8476* 0.7573 10 12.9831* 2.2029 F4 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 306.3* 49.7 10 17.2772* 3.6046 10 5.6508* 0.8632 10 7.9473* 1.5028 TABLE 48 Summary of Organ Height Data Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 7 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=4;SUBSET=ALL LUNG oc r u e> SEX DOSE GROUP M1 NUMBER IN GROUP: MEAN: STANDARD DEV: M4 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT <g> ORGAN WEIGHT <g> 5 722.2 58.0 5 2.1417 0.2399 10 639.0* 71.5 10 2.2129 0.3222 ORGAN-TOBODY WT (%) 5 0.2979 0.0401 ORGAN-TOBRAIN WT RATIO 5 0.9013 0.1183 SEX DOSE GROUP F1 NUMBER IN GROUP MEAN STANDARD DEV 10 0.3472* 0.0412 10 0.9301 0.1290 F4 NUMBER IN GROUP MEAN STANDARD DEV TERMINAL BODY WT (g) ORGAN WEIGHT (g) ORGAN-TOBODY WT <%) ORGAN-TO BRAIN WT RATIO 5 406.9 32.2 5 1.7049 0.1511 5 0.4211 0.0499 5 0.7742 0.0974 10 306.3* 49.7 10 1.6432 0.1425 10 0.5458* 0.0770 10 0.7592 0.0720 TABLE 48 Summary of Organ Weight Data Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 8 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=4;SUBSET=ALL LEFT OVARY SEX DOSE GROUP TERMINAL BODY WT (g) ORGAN WEIGHT (g) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO SEX DOSE GROUP TERMINAL BODY WT (0) ORGAN WEIGHT (g) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO M1 o<r* NUMBER IN GROUP: 5 MEAN: 722.2 STANDARD DEV: 58.0 a F1 NUMBER IN GROUP MEAN STANDARD DEV 5 406.9 32.2 5 0.0646 0.0121 5 0.0159 0.0031 5 0.0292 0.0055 GO M 4 NUMBER IN GROUP: 10 MEAN: 639.0* STANDARD DEV: 71.5 F4 NUMBER IN GROUP MEAN STANDARD DEV 10 306.3* 49.7 10 0.0731 0.0139 10 0.0245* 0.0062 10 0.0338 0.0067 TABLE 48 Summary of Organ Height Data Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 9 TABLE INCLUDES: SEX=ALL ;GROUP=ALL ;WEEKS=ALL DEATH=4;SUBSET=ALL RIGHT OVARY SEX DOSE GROUP M1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) ORGAN WEIGHT (g) 5 722.2 58.0 ORGAN-TO- BODY WT (%) ORGAN-TO- BRAIN WT RATIO SEX DOSE GROUP F1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (0) 5 406.9 32.2 ORGAN WEIGHT (0) 5 0.0607 0.0097 ORGAN-TOBODY WT () ORGAN-TO- BRAIN WT RATIO 5 0.0150 0.0026 5 0.0275 0.0044 M4 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 639.0* 71.5 F4 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 306.3* 49.7 10 0.0781 0.0242 10 0.0262* 0.0096 10 0.0361 0.0114 TABLE 48 Summary of Organ Weight Data Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 10 TABLE INCLUDES: SEX=ALL ;GROUP=ALL ;WEEKS=ALL DEATH=4;SUBSET=ALL SPLEEN SEX DOSE GROUP M1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (0) 5 722.2 58.0 ORGAN WEIGHT (g) 5 0.9636 0.1238 ORGAN-TOBODY WT (%) 5 0.1337 0.0168 ORGAN-TOBRAIN WT RATIO 5 0.4063 0.0660 SEX DOSE GROUP F1 NUMBER IN GROUP MEAN STANDARD DEV TERMINAL BODY WT (0) ORGAN WEIGHT (0) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 5 406.9 32.2 5 0.6205 0.0408 5 0.1531 0.0135 5 0.2808 0.0155 M4 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 639.0* 71.5 10 0.9192 0.3274 10 0.1421 0.0398 10 0.3838 0.1251 F4 NUMBER IN GROUP MEAN STANDARD DEV 10 306.3* 49.7 10 0.5425 0.0914 10 0.1792 0.0311 10 0.2507 0.0442 TABLE 48 Summary of Organ Height Data Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 11 TABLE INCLUDES: SEX=ALL ;GROUP=ALL ;WEEKS=ALL DEATH=4;SUBSET=ALL LEFT TESTIS SEX DOSE GROUP TERMINAL BODY WT (9) ORGAN WEIGHT (9) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO SEX DOSE GROUP TERMINAL BODY WT <g) ORGAN WEIGHT (9) ORGAN-TOBODY WT () ORGAN-TO BRAIN WT RATIO o M1 F1 o Co NUMBER IN GROUP: 55 5 5 NUMBER IN GROUP: 5 Co MEAN: STANDARD DEV: 722.2 58.0 1.6043 0.1032 0.2228 0.0164 0.6747 0.0509 MEAN: STANDARD DEV: 406.9 32.2 Vi N* M 4 F4 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 639.0* 71.5 10 1.7040 0.2865 10 0.2710 0.0609 10 0.7196 0.1362 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 306.3* 49.7 TABLE 48 Summary of Organ Weight Data Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANES0LFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 12 TABLE INCLUDES: SEX=ALL ;GROUP-ALL ;WEEKS=ALL DEATH=4;SUBSET=ALL RIGHT TESTIS SEX DOSE GROUP M1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (0) 5 722.2 58.0 ORGAN WEIGHT (0) 5 1.6110 0.0997 ORGAN-TOBODY WT (%) 5 0.2237 0.0145 ORGAN-TOBRAIN WT RATIO '5 0.6772 0.0428 SEX DOSE GROUP F1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) 5 406.9 32.2 ORGAN WEIGHT (g) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO H4 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 639.0* 71.5 10 1.7805* 0.1392 10 0.2825* 0.0442 10 0.7506 0.0799 F4 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 306.3* 49.7  e -, Co CO TABLE 48 Summary of organ Height Data Week S3 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAHIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 13 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=4;SUBSET=ALL LEFT THYROID/PAR SEX DOSE GROUP H1 NUMBER IN GROUP MEAN STANDARD DEV TERMINAL BODY WT (0) 5 722.2 58.0 ORGAN WEIGHT (0) 5 0.0151 0.0027 ORGAN-TOBODY WT (%) 5 0.0021 0.0003 ORGAN-TOBRAIN WT RATIO 5 00..00001613 SEX DOSE GROUP F1 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (0) 5 406.9 32.2 ORGAN WEIGHT (0) 5 0.0148 0.0045 ORGAN-TOBODY WT (%) ORGAN-TO BRAIN WT RATIO 5 00..00001317 5 0.0068 0.0022 M4 NUMBER IN GROUP MEAN STANDARD DEV 10 639.0* 71.5 10 0.0235* 0.0038 10 0.0037* 0.0004 10 0.0099* 0.0015 F4 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 306.3* 49.7 10 0.0156 0.0053 10 0.0052 0.0021 10 0.0072 0.0024 TABLE 48 Summary of Organ Weight Data Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 14 TABLE INCLUDES: SEX=ALL ;GROUP=ALL ;WEEKS=ALL DEATH=4;SUBSET=ALL RIGHT THYROID/PR SEX DOSE GROUP TERMINAL BODY WT (g) ORGAN WEIGHT (g) ORGAN-TOBODY WT () ORGAN-TOBRAIN WT RATIO SEX DOSE GROUP TERMINAL BODY WT (g) ORGAN WEIGHT (g) ORGAN-TOBODY WT (%) ORGAN-TO- BRAIN WT RATIO M1 o o NUMBER IN GROUP: MEAN: Co STANDARD DEV: Cj 5 722.2 58.0 5 0.0189 0.0044 5 0.0026 0.0004 5 0.0079 0.0016 P1 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 406.9 32.2 5 0.0167 0.0044 5 0.0041 0.0008 5 0.0076 0.0019 M4 F4 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 639.0* 71.5 10 0.0250 0.0061 10 0.0039* 0.0006 10 0.0105 0.0024 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 306.3* 49.7 10 0.0160 0.0064 10 0.0054 0.0025 10 0.0074 0.0029 TABLE 48 Summary of Organ Weight Data Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1*) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 15 TABLE INCLUDES: SEX=ALL;GROUP=ALL;WEEKS=ALL DEATH=4;SUBSET=ALL UTERUS SEX DOSE GROUP TERMINAL BODY WT (g) ORGAN WEIGHT (g) ORGAN-TOBODY WT (*) ORGAN-TOBRAIN WT RATIO SEX DOSE GROUP TERMINAL BODY WT (9) ORGAN WEIGHT (9) ORGAN-TOBODY WT (*) ORGAN-TOBRAIN WT RATIO V' M 1 C NUMBER IN GROUP: CJ MEAN: C i STANDARD DEV: 5 722.2 58.0 F1 NUMBER IN GROUP: MEAN: STANDARD DEV: 5 406.9 32.2 5 1.1161 0.4939 5 0.2806 0.1321 5 0.5103 0.2406 cn M 4 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 639.0* 71.5 F4 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 306.3* 49.7 10 1.1694 0.2972 10 0.3855 0.0989 10 0.5408 0.1387 TABLE 49 Summary of Organ Weight Data Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 1 TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=3;SUBSET=ALL LEFT ADRENAL SEX DOSE GROUP M8 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (0) ORGAN WEIGHT (g) 10 721.2 89.7 10 0.0309 0.0067 ORGAN-TOBODY WT (%) 10 0.0043 0.0007 ORGAN-TOBRAIN WT RATIO 10 0.0128 0.0026 SEX DOSE GROUP F8 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) ORGAN WEIGHT (g) 10 412.5 85.7 10 0.0525 0.0225 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 10 0.0133 0.0068 10 0.0244 0.0115 M9 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 725.1 76.6 10 0.0334 0.0053 10 0.0046 0.0007 10 0.0140 0.0021 F9 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 396.4 49.5 10 0.0435 0.0075 10 0.0111 0.0022 10 0.0212 0.0030 TABLE 49 Summary of Organ Weight Data Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 2 TABLE INCLUDES: SEX=ALL;GR0UP=8,9;WEEKS=ALL DEATH=3;SUBSET=ALL RIGHT ADRENAL SEX DOSE GROUP TERMINAL BODY WT <g) ORGAN WEIGHT (9) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO SEX DOSE GROUP TERMINAL BODY WT (9) ORGAN WEIGHT (9) ORGAN-TOBODY WT (%) ORGAN-TO- BRAIN WT RATIO M8 NUMBER IN GROUP: c- MEAN: STANDARD DEV: Co 10 721.2 89.7 10 0.0320 0.0046 10 0.0045 0.0006 10 0.0132 0.0016 F8 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 412.5 85.7 10 0.0384 0.0086 10 0.0096 0.0031 10 0.0177 0.0042 M9 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 725.1 76.6 10 0.0286 0.0034 10 0.0040 0.0007 10 0.0120 0.0014 F9 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 396.4 49.5 10 0.0379 0.0072 10 0.0096 0.0019 10 0.0186 0.0036 TABLE 49 Summary of Organ Weight Data Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANBSULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 3 TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=3;SUBSET=ALL BRAIN SEX DOSE GROUP M8 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (a) ORGAN (a)WEIGHT 10 721.2 89.7 10 2.4158 0.1977 ORGAN-TOBODY WT (%) 10 0.3385 0.0420 ORGAN-TOBRAIN WT RATIO 10 1.0000 0.0000 SEX DOSE GROUP F8 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (a) ORGAN WEIGHT (a) 10 4 1 2 .5 8 5 .7 10 2.1708 0.1447 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 10 0.5433 0.0978 10 1.0000 0.0000 M9 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 725.1 76.6 10 2.3887 0.0908 10 0.3324 0.0345 10 1.0000 0.0000 F9 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 3 9 6 .4 4 9 .5 10 2.0441* 0.0983 10 0.5221 0.0618 10 1.0000 0.0000 TABLE 49 Summary of Organ Weight Data Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3H T-6316.1 PAGE: 4 TABLE INCLUDES: SEX-ALL;GROUP=8,9;WEEKS=ALL DEATH=3 ;SUBSET=ALL LEFT KIDNEY SEX DOSE GROUP TERMINAL BODY WT <g> ORGAN WEIGHT (g) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO SEX DOSE GROUP TERMINAL BODY WT <g> ORGAN WEIGHT (g) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO o N8 F8 y-* vP r- NUMBER IN GROUP: 10 10 10 10 NUMBER IN GROUP: 10 10 10 10 MEAN: 721.2 2.3570 0.3279 0.9754 MEAN: 412.5 1.4112 0.3461 0.6518 C5 STANDARD DEV: 89.7 0.2803 0.0270 0.0820 STANDARD DEV: 85.7 0.2048 0.0284 0.0994 M9 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 725.1 76.6 10 2.2022 0.3009 10 0.3056 0.0435 10 0.9215 0.1177 F9 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 396.4 49.5 10 1.3218 0.1769 10 0.3353 0.0394 10 0.6460 0.0730 C03380 TABLE 49 Summary of Organ Height Data Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=3;SUBSET=ALL RIGHT KIDNEY SEX DOSE GROUP M8 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT <g) ORGAN HEIGHT (g) 10 721.2 89.7 10 2.3234 0.2897 ORGAN-TOBODY WT <%) 10 0.3234 0.0315 ORGAN-TOBRAN WT RATIO 10 0.9622 0.0962 SEX DOSE GROUP F8 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) ORGAN WEIGHT (g) 10 412.5 85.7 10 1.4370 0.2720 M9 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 725.1 76.6 10 2.2647 0.3230 10 0.3144 0.0491 10 0.9474 0.1257 F9 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 396.4 49.5 10 1.3601 0.2061 Covance 6329-228 3M T-6316.1 PAGE: 5 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 10 0.3507 0.0371 10 0.6636 0.1305 10 0.3449 0.0481 10 0.6642 0.0832 TABLE 49 Summary of Organ Weight Data Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 6 TABLE INCLUDES: SEX=ALL;GROUP=8,9 ;WEEKS=ALL DEATH=3;SUBSET=ALL LIVER SEX DOSE GROUP M8 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) ORGAN WEIGHT la) 10 721.2 89.7 10 20.2343 4.2122 ORGAN-TOBODY WT () 10 2.8067 0.4847 ORGAN-TOBRAIN WT RATIO 10 8.3464 1.4431 SEX DOSE GROUP F8 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT la) ORGAN WEIGHT (9) 10 412.5 85.7 10 11.3196 2.4524 ORGAN-TOBODY WT <%> ORGAN-TOBRAIN WT RATIO 10 2.7467 0.1596 10 5.2305 1.1889 M9 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 725.1 76.6 10 20.3650 2.9205 10 2.8120 0.3074 10 8.5202 1.1115 F9 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 396.4 49.5 10 10.9316 1.4863 10 2.7642 0.2431 10 5.3527 0.7110 TABLE 49 Summary of Organ Weight Data Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 7 TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=3 ;SUBSET=ALL LUNG SEX DOSE GROUP M8 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) ORGAN WEIGHT (g) 10 721.2 89.7 10 2.1758 0.2190 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 10 0.3044 0.0381 10 0.9008 0.0625 SEX DOSE GROUP F8 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) ORGAN WEIGHT (g) 10 412.5 85.7 10 1.6720 0.2500 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 10 0.4216 0.1081 10 0.7736 0.1347 M9 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 725.1 76.6 10 2.0380 0.1979 10 0.2845 0.0431 10 0.8542 0.0877 F9 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 396.4 49.5 10 1.4877 0.1285 10 0.3787 0.0404 10 0.7284 0.0599 o Co Co QO Co TABLE 49 Summary of Organ Weight Data Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 8 TABLE INCLUDES: SEX=ALL;CROUP=8,9 ;WEEKS=ALL DEATH=3:SUBSET=ALL LEFT OVARY SEX DOSE GROUP 'M 8 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) ORGAN WEIGHT (g) 10 721.2 89.7 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO SEX DOSE GROUP F8 NUMBER IN GROUP MEAN STANDARD DEV TERMINAL BODY WT (9) ORGAN WEIGHT (9) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 10 412.5 85.7 10 0.0564 0.0161 10 0.0143 0.0053 10 0.0263 0.0083 M9 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 725.1 76.6 F9 NUMBER IN GROUP MEAN STANDARD DEV 10 396.4 49.5 10 0.0611 0.0183 10 0.0155 0.0047 10 0.0297 0.0081 TABLE 49 Summary of Organ Weight Data Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329 - 2 2 8 3M T-6316.1 PAGE: 9 TABLE INCLUDES: SEX=ALL:GROUP=8,9;WEEKS=ALL DEATH=3;SUBSET=ALL RIGHT OVARY fr8P?00 SEX DOSE GROUP M8 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) 10 721.2 89.7 ORGAN WEIGHT (g) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO SEX DOSE GROUP F8 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (3) ORGAN WEIGHT (9) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 10 412.5 85.7 10 0.0544 0.0176 10 0.0140 0.0059 10 0.0253 0.0090 M9 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 725.1 76.6 F9 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 396.4 49.5 10 0.0624 0.0105 10 0.0160 0.0035 10 0.0305 0.0044 TABLE 49 Summary of Organ Weight Data Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 10 TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=3;SUBSET=ALL SPLEEN SEX DOSE GROUP M8 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (0) ORGAN WEIGHT (g) 10 721.2 89.7 10 1.0371 0.2467 ORGAN-TOBODY WT (%) 10 0.1430 0.0218 ORGAN-TOBRAIN WT RATIO 10 0.4265 0.0763 SEX DOSE GROUP F8 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g> ORGAN WEIGHT (g) 10 412.5 85.7 10 0.5884 0.0964 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 10 0.1446 0.0191 10 0.2721 0.0491 M9 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 725.1 76.6 10 1.1631 0.2368 10 0.1618 0.0364 10 0.4862 0.0943 F9 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 396.4 49.5 10 0.5933 0.1075 10 0.1516 0.0313 10 0.2911 0.0554 TABLE 49 Summary of Organ Weight Data Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 11 TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=3;SUBSET=ALL LEFT TESTIS I- ) Co Co 05 SEX DOSE GROUP M8 NUMBER IN GROUP: MEAN: STANDARD DEV: M9 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (9) ORGAN WEIGHT (9) 10 721.2 89.7 10 1.8105 0.1543 10 725.1 76.6 10 1.7120 0.3999 ORGAN-TOBODY WT (%) ORGAN-TO BRAIN WT RATIO 10 0.2529 0.0240 10 0.7539 0.0873 10 0.2403 0.0685 10 0.7138 0.1556 VO SEX DOSE GROUP F8 NUMBER IN GROUP: MEAN: STANDARD DEV: NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g) 10 412.5 85.7 10 396.4 49.5 ORGAN WEIGHT (g) ORGAN-TO- ORGAN-TOBODY WT BRAIN WT (%) RATIO TABLE 49 Summary of Organ Weight Data Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 12 TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=3;SUBSET=ALL RIGHT TESTIS SEX DOSE GROUP TERMINAL BODY WT (0) ORGAN WEIGHT (0) ORGAN-TOBODY WT () ORGAN-TOBRAIN WT RATIO SEX DOSE GROUP TERMINAL BODY WT (g) ORGAN WEIGHT (g) ORGAN-TOBODY WT {%) ORGAN-TOBRAIN WT RATIO M8 F8 co NUMBER IN GROUP: MEAN: STANDARD DEV: Cm CO 00 M 9 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 721.2 89.7 10 1.8082 0.1769 10 725.1 76.6 10 1.8413 0.2307 10 0.2523 0.0226 10 0.2571 0.0479 10 0.7S36 0.1006 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 0.7703 0.0872 F9 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 412.5 85.7 10 396.4 49.5 TABLE 49 Summary of Organ Weight Data Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covane 6329-228 3M T-6316.1 PAGE: 13 TABLE INCLUDES: SEX=ALL;GR0UP=8,9 ;WEEKS=ALL DEATH=3 ;SUBSET=ALL LF THYROID/PARA SEX DOSE TERMINAL BODY WT ORGAN WEIGHT ORGAN-TO- ORGAN-TOBODY WT BRAIN WT SEX DOSE TERMINAL BODY WT ORGAN WEIGHT ORGAN-TO- ORGAN-TOBODY WT BRAIN WT o GROUP (g) (g) (%) RATIO GROUP <g) (g) (%) RATIO o H8 F8 Co NUMBER IN GROUP: Ch> MEAN: 10 721.2 10 0.0232 10 0.0032 10 0.0096 NUMBER IN GROUP: MEAN: 10 412.5 10 0.0142 10 0.0035 10 0.0066 00 STANDARD DEV: 89.7 0.0067 0.0007 0.0026 STANDARD DEV: 85.7 0.0032 0.0007 0.0017 GS M9 F9 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 725.1 76.6 10 0.0202 0.0050 10 0.0028 0.0008 10 0.0085 0.0021 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 396.4 49.5 10 0.0177 0.0066 10 0.0045 0.0016 10 0.0087 0.0034 TABLE 49 Summary of Organ Weight Data Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 14 TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=3;SUBSET=ALL RT THYROID/PARA TERMINAL ORGAN ORGAN-TO- ORGAN-TO- TERMINAL ORGAN ORGAN-TO- ORGAN-TO- SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT SEX DOSE BODY WT WEIGHT BODY WT BRAIN WT GROUP c (0) (0) (%) RATIO GROUP (0) (0) (%) RATIO o M8 F8 c- NUMBER IN GROUP: 10 10 10 10 NUMBER IN GROUP: 10 10 10 10 Cv MEAN: 721.2 0.0251 0.0035 0.0104 MEAN: 412.5 0.0142 0.0035 0.0066 00 STANDARD DEV: 89.7 0.0079 0.0009 0.0030 STANDARD DEV: 85.7 0.0025 0.0005 0.0013 M9 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 725.1 76.6 10 0.0242 0.0060 10 0.0034 0.0009 10 0.0101 0.0023 F9 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 396.4 49.5 10 0.0147 0.0044 10 0.0037 0.0010 10 0.0072 0.0020 TABLE 49 Summary of Organ Weight Data Week S3 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3H T-6316.1 PAGE: 15 TABLE INCLUDES: SEX-ALL;GROUPs8,9;WEEKS=ALL DEATH=3 ;SUBSET=ALL UTERUS SEX DOSE GROUP H8 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT <g> ORGAN WEIGHT (g) 10 721.2 89.7 ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO SEX DOSE GROUP F8 NUMBER IN GROUP: MEAN: STANDARD DEV: TERMINAL BODY WT (g> ORGAN WEIGHT (g) ORGAN-TOBODY WT (%) ORGAN-TOBRAIN WT RATIO 10 412.5 85.7 10 1.0319 0.1956 10 0.2563 0.0631 10 0.4776 0.1015 M9 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 725.1 76.6 F9 NUMBER IN GROUP: MEAN: STANDARD DEV: 10 396.4 49.5 10 1.0749 0.2526 10 0.2753 0.0763 10 0.5268 0.1225 TABLE 50 Incidence of M acroscopic O bservations Week 4 S a c r i f i c e 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T - 6 3 1 6 . 1 PAGE: 1 TABLE INCLUDES: S E X = A L L ;G R O U P= 1,2, 3, 4 , 5 ; W E E K S = A L L DEATH=1;SUBSET=T ORGAN AND KEYWORD(S) OR PHRASE ** T O P O F LIST ** G E N E R A L C O M M E N T (GC) ........ NO MACROSCOPIC LESIONS L I V E R (L I ) ..................... ** E N D O F LIST ** -- N U M B E R - 0 F - A N I M A L S - A F F E C T E D --SEX: GROUP: -1- -2- -3- -4- -5- -1- -2- -3- -4- -5NUMBER: 5 5 5 5. 5 5 5 5 5 5 NUMBER EXAMINED: 5 5 5 5 5 5 5 5 5 5 5 5 555S 55 55 NUMBER EXAMINED: 5 5 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 S 5 5 co Co Ci CQ TABLE 51 Incidence of Macroscopic Observations Week 4 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 1 TABLE INCLUDES: SEX=AL L ; G R O U P= 8 , 9 ; W E EKS=ALL DEATH=1;SUBSET=T --- N U M B E R - O F - A N I M A L S - A F F E C T E D ---SEX: -- MALE -- -FEMALEGROUP: -8- - 9- -8- -9- ORGAN AND KEYWORD!S) OR PHRASE NUMBER: 5 5 5 5. ** T O P O F LIST ** G E N E R A L C O M M E N T (GC) ........................ ............. N U M B E R EXAMINED: 5 5 5 5 NO MACROSCOPIC LESIONS 5555 L I V E R (LI) ..................................... 5555 NOT REMARKABLE: 5 5 5 5 ** E N D O F L I S T ** TABLE 52 Incidence of Macroscopic Observations Week 8 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M-T6316.1 PAGE: 1 TABLE INCLUDES: SEX=ALL ;GROUP=l,5 ;WEEKS=ALL DEATH=2 ;SUBSET=ALL ORGAN AND KEYWORD(S) OR PHRASE ** TOP OF LIST ** GENERAL COMMENT (GC) ....... e o NO MACROSCOPIC LESIONS Co BONE MARROW SMEAR TAKEN Co LUNG (LU) .................. (0 Co MOTTLED LIVER (LI) ................. LIGHT FOCUS(I)/AREA(S) LARGE MOTTLED DIFFUSELY DARK DEPRESSED FOCUS (I )/AREA (S ) THYMUS (TH) ............... RED FOCUS(I)/AREA(S) -- NUMBER -( SEX: -- MALE-- -FEMALE- GROUP: -1- -5- -1- -5- NUMBER: 5 5 5 5 NUMBER EXAMINED: 5 5 5 5 4 04 1 55S5 NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 4 5 5 5 1000 NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 0 S 2 0100 0502 0001 02 0 2 0100 NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 4 0001 ANIMALS-AFFECTED--- TABLE 52 Incidence of Macroscopic Observations Week 8 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M-T6316.1 PAGE: 2 TABLE INCLUDES: SEX=ALL;GROUP=l,5;WEEKS=ALL DEATH=2;SUBSET=ALL ORGAN AND KEYWORD(S) OR PHRASE ADRENAL, CORTEX (AC) ....... DIFFUSELY RED STOMACH, GL (ST) ........... DARK FOCUS(I)/AREA(S) RED FOCUS (I) /AREA(S) CECUM (CE) ................. DIFFUSELY RED UTERUS (UT) ................ LUMEN FILLED WITH FLUID LN, MANDIBULAR (MN) ........ . DIFFUSELY RED -- N U M B E R - O F - A N I M A L S - A F F E C T E D ---- SEX: -- MALE-- -FEMALE- GROUP: -1- -5- -1- -5- NUMBER: 5 5 5 .5 NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 4 5 5 0100 NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 3 5 4 0101 0100 NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 4 5 5 0100 NUMBER EXAMINED: 0 0 5 5 NOT REMARKABLE: 0 0 5 4 0001 NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 4 5 4 5 1010 TABLE 52 Incidence of Macroscopic Observations Week 8 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M-T6316.1 PAGE: 3 TABLE INCLUDES: SEX=ALL;GROUP=l,5;WEEKS=ALL DEATH=2 ;SUBSET=ALL ORGAN AND KEYWORD(S) OR PHRASE CAVITY, ABDOM (PC) c0 EDEMA END OF LIST ** o. Cv CD 01 -- N U M B E R - O F SEX -- MALE-- -FEMALEGROUP -1- -5- -1- -5NUMBER 5 5 5 5 ANIMALS A F F E C T E D --- NUMBER EXAMINED: 5 5 NOT REMARKABLE: 5 4 5 5 0 10 5 5 0 TABLE 53 Incidence of Macroscopic Observations Week 14 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 1 TABLE INCLUDES: SEX=ALL;GR0UP=1,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL -- NUMB ER -0 F -AN IMALS SEX: .--- MALE- -- ______ ___ _-- FEMALE-- GROUP: -1- -3- -4- O--R---G---A--N---A--ND KE--Y-W--O--R--D--(--S--)-----O---R----P--H--R---A--S--E----------------------- -------- -------------- ** TOP OF LIST ** GENERAL COMMENT (GC) ..................... ----------------- --N--U--MB--E--R: - 3 -5 ------5 ~=--5 ~ 35- - ----5 - 3 -5 - --5- - --5- 55 5 55 555 oo NO MACROSCOPIC LESIONS BONE MARROW SMEAR TAKEN URINARY BLADDER-SMALL LUMEN Co C O KIDNEY (KD) .............................. CD <75 LARGE PELVIS(ES) 22 54 3 424 05 05 05 05 05 05 5 1 05 5555 5 555 NOT REMARKABLE: 4 5 5 5 5 5 5 5 10000000 LIVER (LI) ............................... 5555555 5 NOT REMARKABLE: 4 5 5 5 5 5 5 5 MOTTLED 10000000 THYMUS (TH) .............................. 5 5555555 NOT REMARKABLE: 4 5 5 5 5 5 5 5 MOTTLED 1 00 00 000 STOMACH, GL (ST) ......................... 555 55555 NOT REMARKABLE: 4 4 5 5 4 5 3 4 DARK FOCUS(I)/AREA(S) RED FOCUS (I) /AREA(S) 00000010 11 0 01 011 TABLE 53 Incidence of Macroscopic Observations Week 14 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 2 TABLE INCLUDES: SEX=ALL;GROUP=l,2,3,4;WEEKS=ALL DEATH=3;SUBSET=ALL ORGAN AND KEYWORD(S) OR PHRASE SKIN (SK) ...................... cUK ALOPECIA-FOCAL RED FOCUS(I)/AREA(S) Co C O PROSTATE (PR) .................. LARGE SEMINAL VESICLES (SV) .......... LARGE UTERUS (UT) .................... LUMEN FILLED WITH FLUID OVARY (OV) ..................... CYST(S) --- N U M B E R - O F - A N I M A L S - A F F E C T E D ---- SEX: -------- M A L E -------- -------FEMALE- GROUP: -1- -2- -3- -4- -1- -2- -3- -4- NUMBER: 5 5 5 5 5 5 5 5 NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 4 5 5 4 5 00000010 00010000 NUMBER EXAMINED: 5 5 5 5 0 0 0 0 NOT REMARKABLE: 4 5 5 5 0 0 0 0 10000000 NUMBER EXAMINED: 5 5 5 5 0 0 0 0 NOT REMARKABLE: 4 5 5 5 0 0 0 0 10000000 NUMBER EXAMINED: 0 0 0 0 5 5 5 5 NOT REMARKABLE: 0 0 0 0 5 5 4 5 00000010 NUMBER EXAMINED: 0 0 0 0 5 5 5 5 NOT REMARKABLE: 0 0 0 0 4 5 5 5 000010 00 TABLE 53 Incidence of Macroscopic Observations Week 14 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 3 TABLE INCLUDES: SEX=ALL;GROUP=l ,2,3,4;WEEKS=ALL DEATH=3; SUBSET=ALL ORGAN AND KEYWORD(S) OR PHRASE N UMBER -0F -AN IMA LS SEX: -.--- MALE-- -- -- ---FEMALE-- GROUP: -1- -2- -3- -4- -1- -2- -3- -4- NUMBER: 5 5 5 5 5 5 5 5 LN, MANDIBULAR (MN) ....................... ......... NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 4 3 5 5 5 4 4 5 DIFFUSELY RED RED FOCUS(I)/AREA(S) ** END OF LIST ** 12000010 00000100 003398 TABLE 54 Incidence of Macroscopic observations Week 14 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 1 TABLE INCLUDES: SEX=ALL;GROUP=8,9;WEEKS=ALL DEATH=2;SUBSET=T ORGAN AND KEYWORD(S) OR PHRASE ** TOP OF LIST ** GENERAL COMMENT (GC) ....... Co Co NO MACROSCOPIC LESIONS CD LIVER (LI) ................. CD ** END OF LIST ** -- NUMBER-OF-ANIMALS-AFFECTED -- SEX: -- MALE-- -FEMALE- H GROUP: -8- -9- -8- -9- NUMBER: 5 5 5 5 NUMBER EXAMINED: 5 5 5 5 55 5 5 NUMBER EXAMINED: 5 5 S 5 NOT REMARKABLE: 5 5 5 5 TABLE 55 Incidence of Macroscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 1 C03400 TABLE INCLUDES: SEX=ALL ;GR0UP=1,4;WEEKS=ALL DEATH=4 ;SUBSET=ALL ORGAN AND KEYWORD(S) OR PHRASE ** TOP OF LIST ** GENERAL COMMENT (GC) ..... NO MACROSCOPIC LESIONS BLOOD FILM TAKEN DISCHARGE-OCULAR MALOCCLUSION BONE MARROW SMEAR TAKEN TEETH MISSING/BROKEN/WORN BRAIN (BR) ............... COMPRESSED (HYPOTHALAMUS) LUNG (LU) ................ LIGHT FOCUS(I)/AREA!S) LIVER (LI) ............ LIGHT FOCUS (I) /AREA(S) MOTTLED -- N U M B E R - 01 F SEX:: -MALE-- -FEMALEGROUP:: -1- -4- -1- -4NUMBER: 5 10 5 10 A N I M A L S - A F F E C T E D -- NUMBER EXAMINED: NUMBER EXAMINED NOT REMARKABLE: NUMBER EXAMINED: NOT REMARKABLE: NUMBER EXAMINED: NOT REMARKABLE: 5 10 33 5 10 01 04 5 10 03 5 10 59 01 5 10 5 10 00 5 10 5 10 00 00 5 10 21 5 10 00 02 5 10 01 5 10 58 02 5 10 58 02 5 10 49 01 10 TABLE 55 Incidence of Macroscopic observations Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=l,4;WEEKS=ALL DEATH=4;SUBSET=ALL ORGAN AND KEYWORD (S) OR PHRASE -- N U M B E R - O F - A N I M A L S SEX: -MALE-- -FEMALEGROUP: -1- -4- -1- -4NUMBER: 5 10 5 10 LN, MESENTERIC (MS) ..................... DIFFUSELY RED ADRENAL, CORTEX (AC) .................... DARK FOCUS(I)/AREA(S) PITUITARY (PI) .......................... MASS(ES) DARK FOCUS (I) /AREA(S) RED FOCUS (I)/AREA(S) STOMACH, GL (ST) ........................ DARK FOCUS(I)/AREA(S) MAMMARY, FEMALE (MF) .................... MASS(ES) CYST(S) NOT REMARKABLE: NOT REMARKABLE: NOT REMARKABLE: NOT REMARKABLE: NOT REMARKABLE: 5 10 5 10 00 5 10 4 10 10 5 10 58 02 00 00 5 10 59 01 00 00 00 00 5 10 59 01 5 10 5 10 00 5 10 47 02 10 01 5 10 44 16 5 10 48 01 12 Covance G329-212 3M T-6316.1 PAGE: 2 A F F E C T E D -- TABLE 55 Incidence of Macroscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GR0UP=1,4;WEEKS=ALL DEATH=4;SUBSET=ALL ORGAN AND KEYWORD(S) OR PHRASE -- N U M B E R - O F - A N I M A L S SEX: -- MALE-- -FEMALEGROUP: -1- -4- -1- -4NUMBER: 5 10 5 10 UTERUS (UT) ........................ LUMEN FILLED WITH FLUID CYST(S) THICKENED WALL OVARY (OV) ......................... CYST(S) LN, MANDIBULAR (MN) ................ DIFFUSELY RED CAVITY, ABDOM (PC) ................. MASS(ES) TAIL (TI) .......................... RAISED AREA(S) ** END OF LIST ** NOT REMARKABLE: NOT REMARKABLE: NOT REMARKABLE: NOT REMARKABLE: NOT REMARKABLE: 00 00 00 00 00 00 00 00 5 10 49 11 5 10 5 10 00 5 10 5 10 00 5 10 38 11 10 01 5 10 59 01 5 10 4 10 10 5 10 59 01 5 10 59 01 Covance 6329-212 3M T-6316.1 PAGE: 3 A F F E C T E D -- TABLE 56 Incidence of Macroscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX-ALL;GROUP-8, 9;WEEKS-ALL DEATH-3;SUBSET-ALL ORGAN AND KEYWORD(S ) OR PHRASE ** TOP OF LIST ** ^ GENERAL COMMENT (GC) ..... O NO MACROSCOPIC LESIONS f - MALOCCLUSION TT ANIMAL OBESE W * BONE MARROW SMEAR TAKEN Q TEETH MISSING/BROKEN/WORN C O BRAIN (BR) ............... COMPRESSED (HYPOTHALAMUS) LUNG (LU) ................ LIGHT FOCUS(I)/AREA(S ) KIDNEY (KD) ........... LARGE PELVIS(ES) CYST (S) SEX; -MALE-- -FEMALE- GROUP: -8- -9- -8- -9- NUMBER: 10 10 1 1 1o 1 1-* 1O NUMBER EXAMINED: 10 10 10 10 l422 0011 7301 10 10 10 10 0010 NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 10 9 0001 NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 9 10 0010 NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 9 10 9 0100 0001 Covance 6329-228 3M T-6316.1 PAGE: 1 003404 TABLE 56 Incidence of Macroscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX-ALL;GROUP-8, 9;WEEKS-ALL DEATH-3;SUBSET-ALL ORGAN AND KEYWORD(S) OR PHRASE NUMBER-( SEX: -MALE-- -FEMALEGROUP: -8- -9- -8- -9NUMBER: 10 10 10 10 LIVER (LI) ............................ LIGHT FOCUS(I )/AREA(S ) MOTTLED RED FOCUS(I)/AREA(S) SPLEEN (SP) ........................... LIGHT FOCUS (I )/AREA (S ) ADRENAL, CORTEX (AC) .................. LARGE PITUITARY (PI) ........................ DARK FOCUS(I)/AREA(S ) LARGE DIFFUSELY RED RED FOCUS(I)/AREA(S ) 10 10 10 10 NOT REMARKABLE: 8 10 9 10 1000 1000 00l0 10 10 10 10 NOT REMARKABLE: 10 9 10 10 0100 10 10 10 10 NOT REMARKABLE: 10 10 9 10 0010 10 10 10 10 NOT REMARKABLE: 10 9 5 8 0010 0142 0001 0021 Covance 6329-228 3M T-6316.1 PAGE: 2 A F F E C T E D -- oo Co o& cn TABLE 56 Incidence of Macroscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T - 6 3 1 6 . 1 PAGE: 3 TABLE INCLUDES: SEX=ALL;GROUP-8, 9,-WEEKS-ALL DEATH-3;SUBSET-ALL ORGAN AND KEYWORD(S) OR PHRASE NUM BER-OF-ANIMALS-AFFECTED -- SEX: -- MALE-- -FEMALE- GROUP: -8- -9- -8- -9- NUMBER: 10 10 ip 1 1IlHo* STOMACH, GL (ST) ........................... DARK FOCUS(I)/AREA!S) SKIN (SK) .................................. CRUSTED AREA(S ) MAMMARY, FEMALE (MF) ....................... MASS(ES) CYST(S) UTERUS (UT) ................................ CYST(S) TESTIS (TE) ................................ SMALL 10 10 10 10 NOT REMARKABLE: 10 10 8 10 0020 10 10 10 10 NOT REMARKABLE: 10 10 10 9 0001 0 NOT REMARKABLE: 0 0 10 10 065 0012 0034 0 NOT REMARKABLE: 0 0 10 10 0 10 9 0001 10 10 0 0 NOT REMARKABLE: 10 9 0 0 0100 TABLE 56 Incidence of Macroscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1*) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-228 3M T-6316.1 PAGE: 4 TABLE INCLUDES: SEX-ALL;GROUP-8, 9;WEEKS-ALL DEATH-3;SUBSET-ALL ORGAN AND KEYWORD(S) OR PHRASE -- N U M B E R - O F - A N I M A L S - A F F E C T E D --SEX: --MALE-- -FEMALEGROUP: -8- -9- -8- -9NUMBER: 10 10 10 10 LN, AXILLARY (AX) .......... DIFFUSELY RED LN, MANDIBULAR (MN) ........ DIFFUSELY RED MOTTLED ** END OF LIST ** NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 9 10 0010 NUMBER EXAMINED: 10 10 10 10 NOT REMARKABLE: 10 10 8 7 0022 0001 TABLE 57 Incidence of M icroscopic O bservations Week 8 S a c r i f i c e 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (9 8 .1 % ) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=l,5;WEEKS=ALL DEATH=2;FIND=ALL;SUBSET=ALL NUM BER-1 SEX: -- MALE-- -FEMALEGROUP: -1- -5- -1- -5- ORGAN AND FINDING DESCRIPTION --------- --------------N-U-M--B-E-R-:- 5 5 _c 5 _--_ _ =5_ ** TOP OF LIST ** EYE (EY) .................................. 55 5 5 NOT REMARKABLE: 5 5 5 5 (-1 BRAIN (BR) ................................. Co 55 55 NOT REMARKABLE: 5 5 5 5 O SPINAL CORD (SC) ........................... ri KIDNEY (KD) ................................ -- CAST, PROTEINACEOUS -- CYST -- MINERALIZATION, TUBULAR -- NEPHROPATHY, CHRONIC PROGRESSIVE LIVER (LI ) ................................. -- HEMORRHAGE -- HYPERTROPHY, HEPATOCELLULAR -- INFLAMMATION, CHRONIC -- NECROSIS, HEPATOCELLULAR -- VACUOLATION, HEPATOCELLULAR 5555 NOT REMARKABLE: 5 5 5 5 5555 NOT REMARKABLE: 3 4 5 4 1000 I100 0001 0100 5 5 55 NOT REMARKABLE: 3 0 4 0 03 01 05 15 2000 03 01 0100 Covance 6329-212 3M T - 6 3 1 6 . 1 PAGE: 1 TABLE 57 Incidence of M icroscopic O bservations Week 8 S a c r i f i c e 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (9 8 .1 % ) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T - 6 3 1 6 . 1 PAGE: 2 TABLE INCLUDES: SEX-ALL;GROUPAI,5,-WEEKS=ALL DEATH=2; FINOALL; SUBSET=ALL ORGAN AND FINDING DESCRIPTION THYMUS (TH) ...... ofv?-;S -- HEMORRHAGE Cj LN, MESENTERIC (MS) o Q O -- HEMORRHAGE SPLEEN (SP) ...... PITUITARY (PI) ADRENAL, CORTEX (AC) ADRENAL, MEDULLA (MA) -- N U M B E R - O F SEX: -- MALE-- -FEMALEGROUP: -1- -5- -1- -5NUMBER: 5 5 5 5 NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 4 5 5 3 1002 NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 4 S 5 5 1000 NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 ANIMALS-AFFECTED TABLE 57 Incidence of M icroscopic O bservations Week 8 S a c r i f i c e 1 0 4 -WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (9 8 .1 % ) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RA.TS o e- o& TABLE INCLUDES: SEX=ALL;GROUP=l,5;WEEKS=ALL DEATH=2;FIND=ALL;SUBSET=ALL ORGAN AND FINDING DESCRIPTION STOMACH, N O N G L (SU) .......... STOMACH, G L (ST) ............. . D U O D E N U M (D U ) .................. J E J U N U M (JE) ................... P A N C R E A S (PA) .................. E P I D I D Y M I D E S (EP) ............. SEX: -- MALE -- -FEMALEGROUP: -1- - 5- -1- -5NUMBER: 5 5 5 5 NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 NUMBER EXAMINED: 5 5 5 5 NOT REMARKABLE: 5 5 5 5 NUMBER EXAMINED: 5 5 0 0 NOT REMARKABLE: 5 5 0 0 Covance 6329-212 3M T - 6 3 1 6 . 1 PAGE: 3 TABLE 57 Incidence of M icroscopie O bservations Week 8 S a c r i f i c e X0 4 -WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE ( 9 8 . 1 i | N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T - 6 3 1 6 . 1 PAGE: 4 TABLE INCLUDES: SEX=ALL;GROUP=l,5;WEEKS=ALL DEATH=2;FIND=ALL; SUBSET=ALL ORGAN AND FINDING DESCRIPTION --- N U M B E R - O F - A N I M A L S - A F F E C T E D ---- SEX: -- M A L E -- -FEMALE- ' GROUP: -1- -5- -1- -5- NUMBER: 5 5 5 5 T E S T I S (TE) ........................ O O C o O V A R Y (OV) .......................... H V A G I N A (VA) ........................ O 5500 NOT REMARKABLE: 5 5 0 0 005 5 NOT REMARKABLE: 0 0 5 5 0055 NOT REMARKABLE: 0 0 5 5 U T E R U S (UT) ........................ 0001 NOT REMARKABLE: 0 0 0 0 -- DILATATION 0001 LN, M A N D I B U L A R (MN) ............... ........................ N U M B E R EXAMINED: 1 0 1 0 NOT REMARKABLE: 0 0 0 0 -- HEMORRHAGE 10 10 LN, M E D I A S T I N A L (ML) ............. ........................ N U M B E R EXAMINED: 0 0 0 1 MOT REMARKABLE: 0 0 0 0 -- HEMORRHAGE 0001 CAVITY, A B D O M (PC) ................ 0100 NOT REMARKABLE: 0 0 0 0 -- EDEMA 0100 ** E N D O F LIST TABLE 58 Incidence of M icroscopic O bservations Week 14 S a c r i f i c e 1 0 4 -WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (9 8 .1 % ) N-ETHYL FERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T - 6 3 1 6 . 1 PAGE: I C-v & TABLE INCLUDES: S E X - A L L ;G R O U P - 1 , 2 , 3 , 4 ; W E E K S - A L L DEATH-3;FIND-ALL;SUBSET-ALL N U M B E R -0 F -ANIMA L S AFFECTED SEX: ----M A L E --- GROUP: -1- -2- -3- -4- --- F E M A L E -- -1- _ 4OL_ -3- -4- ORGAN AND FINDING DESCRIPTION NUMBER: -- -- -- -- ----- -- -- -- ---------------------------------- ---------------- ------------------------------------ -- -- -- --------_ _ _ _ _ _ _ _ _ ** T O P O F LIST ** E Y E (EY) ................................ ..................... N U M B E R E X AMINED: NOT REMARKABLE: 5 -- *-- 5 5 5 ~= -- 5 5 5 -- as-- 5 5 5. 5 -- = -- -- SB-- 55 55 5 -- SS-- 5 5 5 -- SS-- 5 5 5 --= -- 5 5 B R A I N (BR) ............................. 55555555 NOT REMARKABLE: 5 5 5 5 5 5 5 5 K I D N E Y (KD) .................................................. N U M B E R EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 3 3 5 5 5 5 4 4 -- CAST, PROTEINACEOUS -- CYST -- DEGENERATION, TUBULAR -- DILATATION, PELVIC -- HYPERPLASIA, TRANSITIONAL CELL -- INFLAMMATION, CHRONIC -- MINERALIZATION, TUBULAR -- PYELONEPHRITIS 1 0 00 00 0 0 00000001 0 1 00 00 0 0 10000000 1 0 000 0 0 0 01000000 00000010 10000000 L I V E R (LI) ................................................... N U M B E R E XAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 3 1 1 0 3 1 3 2 -- HEMATOPOIESIS, EXTRAMEDULLARY -- HEMORRHAGE -- HYPERTROPHY, HEPATOCELLULAR -- INFLAMMATION, CHRONIC -- VACUOLATION, HEPATOCELLULAR 1000 0 00 0 10 00 1 000 0 135 0 002 1 1 3 0 1 4 rt i. 2 02210000 TABLE 58 Incidence of M icroscopic O bservations Week 14 S a c r i f i c e 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (9 8 .1 % ) N-ETHYL PERFLUOROOCTANE3ULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: S E X A L L ; G R O U P = l , 2, 3, 4 ; W E E K S = A L L DEATH=3; FIND=ALL;SUBSET=ALL o O c- pU W ORGAN AND FINDING DESCRIPTION .......... LN, M E S E N T E R I C (MS) ..................... -- HEMORRHAGE -- INFILTRATE, MACROPHAGE, PIGMENTED S P L E E N (SP) .............................. ADRE N A L , C O R T E X (AC) ..................... -- VACUOLATION ADRENAL, M E D U L L A (MA) P A N C R E A S (PA) -- FIBROSIS -- PIGMENT T E S T I S (TE) . --ATROPHY/DEGENERATION O V A R Y (OV) ................ -- HEMORRHAGE ** E N D O F LIST ** NUMB E R - 0 F - ANI M A L S SEX: GROUP: -1- -2- - 3- -4- -1- -2- -3- -4NUMBER: 5 5 5 5 5 5 5 5 NUMBER EXAMINED: 4 5 5 5 5 5 5 5 NOT REMARKABLE: 4 5 4 5 5 5 4 5 0 0 10 0 0 1 0 0 000 0 010 NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 3 2 4 4 5 5 5 4 23110001 NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 5 5 5 5 5 5 5 5 NUMBER EXAMINED: 5 5 5 5 5 5 5 5 NOT REMARKABLE: 4 5 4 5 5 5 5 5 10000000 10100000 NUMBER EXAMINED: 5 5 5 5 0 0 0 0 NOT REMARKABLE: 5 5 4 5 0 0 0 0 00100000 NUMBER EXAMINED: 0 0 0 0 5 5 5 5 NOT REMARKABLE: 0 0 0 0 5 5 5 4 00000001 Covance 6329-212 3M T - 6 3 1 6 . 1 PAGE: 2 TABLE 60 Incidence of S ev erity of M icroscopic O bservations Week fi S a c r i f i c e 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMI DO ETHANOL IN RATS Covance 6329-212 3M T 6 3 1 6 . 1 PAGE: 1 cwroo TABLE INCLUDES: SEX=ALL;GROUP=l,5;WEEKS=ALL DEATH=2; FIND=ALL; SU8SET=T -- N UM BERSEX: -- MALE-- -FEMALEGROUP: -1- -5- -1- -5- ORGAN/TISSUE EXAMINED -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- ----------------- - -- -- -- -- -- -- -- -- -- -- -- ** T O P O F L I S T ** L I V E R (LI) ................................ -- HEMATOPOIESIS, EXTRAMEDULLARY NUMBER: 5 -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- -- ** -> TL> MN> 5 5 5 0.0 5 -- -- 5 5 5 0.0 5 -- SS-- 5 5 5 0.0 5 --= -- 5 5 S 0.0 -- HEMORRHAGE -> 1> 3> TL> MN> 5 0 0 5 0.0 2 3 0 5 0.6 5 0 0 5 0.0 4 0 1 5 0.6 -- HYPERTROPHY, HEPATOCELLULAR -> 1> 3> 4> TL> MN> 5 0 0 0 5 0.0 0 0 5 0 5 3.0 4 1 0 0 5 0.2 0 0 4 1 5 3.2 -- INFLAMMATION, CHRONIC ** CONTINUED ON NEXT PAGE ** "> 3 1> 2 TL> 5 MN> 0.4 5 0 5 0.0 5 0 S 0.0 5 0 5 0.0 ANIMALS-AFFECTED  Il> ? 0 9 TABLE 60 Incidence of S everity of M icroscopic O bservations Week 8 S a c r i f i c e 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=l, 5;WEKS=ALL DEATH-2;FIND-ALL;SUBSET-T ORGAN/TISSUE EXAMINED ** FROM P R E V I O U S PAGE * L I V E R (L I ) ...................................... -- NECROSIS, HEPATOCELLULAR -- VACUOLATION, HEPATOCELLULAR ** E N D O F L IS T ** -- mum B E R - SEX: -- M A L E -- -FEMALE- GROUP: -1- -5- -1- -5- NUMBER: 5 5 5 5 -> 1> 3> TL> MN> 5 5 0 0 5 0.0 5 2 1 2 5 1.4 5 5 0 0 5 0.0 5 4 0 1 5 0.6 -> 1> TL> MN> 5 0 5 0.0 4 1 5 0.2 5 0 5 0.0 5 0 5 0.0 Covance 6329-212 3M T 6 3 1 6 . 1 PAGE: 2 A F F E C T E D --- G C- v\ TABLE 61 Incidence of S everity of M icroscopic O bservations Week 14 S a c r i f i c e 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=l, 2,3,4;WEEKS=ALL DEATH=3;FIND-ALL;SUBSET=T ORGAN/TISSUE EXAMINED ** T O P O F LIST ** L I V E R (LI) ........................... -- HEMATOPOIESIS, EXTRAMEDULLARY -- HEMORRHAGE -- HYPERTROPHY, HEPATOCELLULAR -- INFLAMMATION, CHRONIC * * CONTINUED ON NEXT PAGE 4 4 -- SEX: N UM B ER -O F-A N IM A LS ----M A L E -- -- -- -- FEMALE-- GROUP: -1- NUMBER: 5 5 5 & 5 5 5 5 -- -= - -as- -as- --s- -s- NUMBER EXAMINED: -> 1> TL> MN> 5 4 1 5 0.2 5 5 0 5 0.0 5 5 0 5 0.0 5 5 0 5 0.0 5 5 0 5 0.0 5 5 0 S 0.0 5 5 0 5 0.0 5 5 0 5 0.0 -> 1> 3> TL> MN> 4 1 0 5 0.2 5 0 0 5 0.0 5 0 0 5 0.0 5 0 0 5 0.0 4 0 1 5 0.6 5 0 0 5 0.0 5 0 0 5 0.0 5 0 0 5 0.0 -> 1> 2> 3> TL> MN> 5 0 0 0 5 0.0 4 1 0 0 5 0.2 2 1 1 1 5 1.2 0 0 4 1 5 2.2 5 0 0 0 5 0.0 5 0 0 0 5 0.0 5 0 0 0 5 0.0 3 0 2 0 5 0.8 -> 1> 2> TL> MN> 4 0 1 5 0.4 4 1 0 5 0.2 2 3 0 5 0.6 5 0 0 5 0.0 4 1 0 5 0.2 1 4 0 5 0.8 3 2 0 5 0.4 3 2 0 5 0.4 Covance 6329-212 3M T 6 3 1 6 .1 PAGE: 1 TABLE 61 Incidence of S ev erity of M icroscopic O bservations Week 14 S a c r i f i c e 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS co cn TABLE INCLUDES: SEX=ALL;GROUP=l,2,3, 4;WEEKS=ALL D E A T H " 3; FINDALL; S U B S E T ! -- NUM R -O F-A N IM A LS SEX: --------M A L E -- -- -- -- FEMALE-- GROUP: -1- -2- -3- -4- -1- -2- -3- -4- ORGAN/TISSUE EXAMINED NUMBER: 5 ----------------- -- ----------------------------------- -------------------------- --------3 -- ** FROM P R E V I O U S PAGE ** L I V E R (L I ) ........................................ .......... N U M B E R E X A M I N E D : -- NECROSIS, HEPATOCELLULAR -> 5 5 TL> 5 MN> 0.0 5 -= -- 5 5 5 0.0 5 --= -- 5 5 5 0.0 5 --SS-- 5 5 5 0.0 5 --= -- 5 5 5 0.0 5 -- ss-- 5 5 5 0.0 5 --= -- 5 5 5 0.0 5 --SS-- 5 5 5 0.0 -- VACUOLATION, HEPATOCELLULAR ** E ND O F LIST -> 1> 2> 3> TL> MN> 5 0 0 0 5 0.0 3 1 1 0 5 0.6 3 1 0 1 5 0.8 4 1 0 0 5 0.2 5 0 0 0 5 0.0 5 0 0 0 5 0.0 5 0 0 0 5 0.0 5 0 0 0 5 0.0 Covance 6329-212 3M T 6 3 1 6 .1 PAGE: 2 <> c e.> a TABLE 59 Incidence of Microscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 1 TABLE INCLUDES: SEX-ALL;GROUP-1,4;WEEKS-ALL DEATH-4;FIND-ALL;SUBSET-ALL ORGAN AND FINDING DESCRIPTION ** TOP OF LIST ** EYE (EY) ...... -- NUMBER-OF-ANIMALS-AFFECTED -- SEX: -- MALE-- -FEMALEGROUP: -1- -4- -1- -4NUMBER: 5 10 5 10 NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 BRAIN (BR) -- COMPRESSION, VENTRAL LIVER (LI) ............. -- DEGENERATION, CYSTIC -- FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC -- FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC -- HYPERPLASIA, BILE DUCT -- HYPERTROPHY, HEPATOCELLULAR -- INFLAMMATION, CHRONIC -- NECROSIS, HEPATOCELLULAR -- STASIS, BILE -- VACUOLATION, HEPATOCELLULAR -- B-ADENOMA, HEPATOCELLULAR KIDNEY (KD) ................................... -- CAST, PROTEINACEOUS -- INFLAMMATION, CHRONIC ** CONTINUED ON NEXT PAGE ** NUMBER EXAMINED: NOT REMARKABLE: NUMBER EXAMINED: NOT REMARKABLE: NUMBER EXAMINED: NOT REMARKABLE: 5 10 5 10 00 5 10 10 00 00 10 00 0 10 31 01 01 09 00 5 10 07 31 00 5 10 58 02 5 10 42 01 01 00 01 07 11 02 02 02 01 5 10 15 03 20 C Co 05 TABLE 59 Incidence of Microscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1*) N-ETHYL PERFLUOROOCTANESULFQNAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 2 TABLE INCLUDES: SEX-ALL;GROUP-1,4;WEEKS-ALL DEATH-4 ;FIND-ALL;SUBSET-ALL --- N U M B E R - 1 F - A N I M A L S - A F F E C T E D SEX: -- MALE-- -FEMALEGROUP: -1- -4- -1- -4- ORGAN AND FINDING DESCRIPTION NUMBER: 5 10 5 10 ** FROM PREVIOUS PAGE ** KIDNEY (KD) .......................... .............. NUMBER EXAMINED: NOT REMARKABLE: 5 10 07 5 10 15 -- MINERALIZATION, PELVIC -- MINERALIZATION, TUBULAR -- NEPHROPATHY, CHRONIC PROGRESSIVE 1122 0021 2100 SPLEEN (SP) .......................... 5 10 NOT REMARKABLE: 5 10 5 10 5 10 PANCREAS (PA) ....................................... NUMBER EXAMINED: 5 10 NOT REMARKABLE: 4 8 -- ATROPHY, ISLET CELLS -- FIBROSIS -- INFLAMMATION, CHRONIC -- PIGMENT 10 01 01 01 ADRENAL, CORTEX (AC) ................... 5 10 NOT REMARKABLE: 3 9 -- ANGIECTASIS -- DEGENERATION, CYSTIC, CORTICAL -- FIBROSIS -- HEMORRHAGE -- HYPERTROPHY, CORTICAL CELL -- 'VACUOLATIQN 00 10 00 10 10 11 5 10 5 10 00 00 00 00 5 10 18 20 02 01 40 00 00 GWroo 1 111 1 1111111 11 1 1 111 1 1 1111111111 1 1 1 1 1 1111 1 1 1 111 TABLE 59 Incidence of Microscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX-ALL;GROUP-1,4;WEEKS-ALL DEATH-4;FIND-ALL;SUBSET-ALL NUM BER-1 SEX: -- MALE-- -FEMALEGROUP: -1- -4- -1- -4- ORGAN AND FINDING DESCRIPTION -- ---- ------- NUMBER: 5 10 5 10 --as-- ---- --=" ADRENAL, MEDULLA (MA) ...................... ........ NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 9 5 10 5 10 -- HEMORRHAGE -- B-PHEOCHROMOCYTGMA 0100 0100 LN, MESENTERIC (MS) ........................ 5 10 NOT REMARKABLE: 5 9 5 10 59 -- HEMORRHAGE -- INFILTRATE, MACROPHAGE, PIGMENTED 0101 0100 OVARY (OV) ................................. 0 0 5 10 NOT REMARKABLE: 0 0 5 7 -- CYST -- HEMORRHAGE 0002 0002 TESTIS (TE) ................................ 5 10 0 0 NOT REMARKABLE: 5 9 0 0 -- ATROPHY/DEGENERATION 0100 BONE, FEMUR (FE) ........................... 5 10 NOT REMARKABLE: 5 10 5 10 5 10 Covance 6329-212 3M T-6316.1 PAGE: 3 TABLE 59 Incidence of Microscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX-ALL;GROUP-1,4;WEEKS-ALL DEATH-4;FIND-ALL;SUBSET-ALL ORGAN AND FINDING DESCRIPTION MARROW, FEMUR (FM) .......... NUM BER-1 SEX: -- MALE-- -FEMALE- GROUP: -1- -4- -1- -4- NUMBER: --*5-- 10 5 10 5 10 NOT REMARKABLE: 5 10 5 10 5 10 MARROW, STERNUM (SE) ........ c - BONE, STERNUM (SB) .......... it* THYMUS (TH) ................. -- FIBROSIS -- HEMORRHAGE SPINAL CORD (SC) ............ 5 10 NOT REMARKABLE: 5 10 5 10 5 10 5 10 NOT REMARKABLE: 5 10 5 10 5 10 NOT REMARKABLE: NOT REMARKABLE: 59 49 00 10 5 10 5 10 5 10 59 01 00 5 10 5 10 LN, MANDIBULAR (MN) ......... -- HEMORRHAGE NOT REMARKABLE: 5 10 49 11 5 10 4 10 10 Covance 6329-212 3M T-6316.1 PAGE: 4 TABLE 59 Incidence of Microscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFQNAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX-ALL;GROUP-1,4;WEEKS-ALL DEATH-4;FIND-ALL;SUBSET-ALL ORGAN AND FINDING DESCRIPTION LUNG (LU) ......................... ......... -- HYPERPLASIA, MEDIAL, ARTERIAL/ARTERIOLAR o -- MINERALIZATION, VASCULAR -- INFILTRATE, MACROPHAGE, ALVEOLAR -- INFILTRATE, MACROPHAGE, PIGMENTED c.-< HEART (HT) .................................. -- DEGENERATION/NECROSIS MUSCLE, SKELETAL (SM) ....................... NUM BER-1 SEX: -- MALE-- -FEMALE- GROUP: -1- -4- -1- -4- NUMBER: 5 10 5 10 --a-- 5 10 NOT REMARKABLE: 4 7 5 10 48 NOT REMARKABLE: 01 11 11 00 5 10 59 00 00 12 01 5 10 5 10 0100 5 10 NOT REMARKABLE: 5 10 5 10 5 10 NERVE, SCIATIC (SN) ......................... 5 10 NOT REMARKABLE: 5 10 5 10 5 10 THYROID (TY) ................................ -- CYST, FOLLICULAR -- CYST, ULTIMOBRANCHIAL -- B-ADENOMA, C-CELL -- B-ADENOMA, FOLLICULAR CELL NOT REMARKABLE: 5 10 37 01 22 00 00 5 10 33 00 26 01 01 Covance 6329-212 3M T-6316.1 PAGE: 5 A F F E C T E D -- TABLE 59 Incidence of Microscopie Observations Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance G329-212 3M T-6316.1 PAGE: 6 TABLE INCLUDES: SEX-ALL;GROUP-1, 4;WEEKS-ALL DEATH-4;FIND-ALL;SUBSET-ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - O F - A N I M A L S - A F F E C T E D -- SEX: -- MALE-- -FEMALE- * GROUP: -1- -4- -1- -4- NUMBER: 5 10 5 10 PARATHYROID (PT) .................. -- HYPERPLASIA TRACHEA (TR) ...................... to ESOPHAGUS (ES) .................... NOT REMARKABLE: NOT REMARKABLE: 5 10 4 10 10 5 10 5 10 59 59 00 5 10 5 10 5 10 NOT REMARKABLE: 5 10 5 10 5 10 PITUITARY (PI) ...................................... NUMBER EXAMINED: NOT REMARKABLE: -- CYST -- HEMORRHAGE -- HYPERPLASIA -- B-ADENOMA SALIV GL, MANDIB (SG) ............. ................. NUMBER EXAMINED: NOT REMARKABLE: 5 10 34 00 02 03 23 5 10 5 10 5 10 35 01 01 00 24 5 10 5 10 TABLE 59 Incidence of Microscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 7 TABLE INCLUDES: SEX-ALL;GROUP-1,4 ;WEEKS-ALL DEATH-4;FIND-ALL;SUBSET-ALL ORGAN AND FINDING DESCRIPTION N U M B E R - 1O F - A N I M A L S - A F F E C T E D -SEX: -- MALE-- -FEMALEGROUP: -1- -4- -1- -4NUMBER: 5 10 5 10 HARDERIAN GLAND (HG) .................. -- INFLAMMATION, CHRONIC O O STOMACH, GL (ST) ...................... Co -- EDEMA to -- EROSION CO STOMACH, NONGL (SU) ................... NOT REMARKABLE: NOT REMARKABLE: NOT REMARKABLE: 5 10 5 10 00 5 10 59 00 01 5 10 5 10 5 10 59 01 5 10 48 10 02 5 10 5 10 DUODENUM (DU) ......................... 5 10 NOT REMARKABLE: 5 10 5 10 5 10 JEJUNUM (JE) .......................... 5 10 NOT REMARKABLE: 5 10 5 10 5 10 ILEUM (IL) ............................ 5 10 NOT REMARKABLE: 5 10 5 10 5 10 TABLE 59 Incidence of Microscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 8 TABLE INCLUDES: SEX=ALL;GROUP-1,4;WEEKS-ALL DEATH-4;FIND-ALL;SUBSET-ALL ORGAN AND FINDING DESCRIPTION N U M B E R - O( F - A N I M A L S - A F F E C T E D -SEX: -- MALE-- -FEMALEGROUP: -1- -4- -1- -4NUMBER: 5 10 5 10 CECUM (CE) ............. ............. 5 10 NOT REMARKABLE: 5 10 5 10 5 10 COLON (CO) ........................... .............. NUMBER EXAMINED: NOT REMARKABLE: -- PARASITISM RECTUM (RE) .......................... NOT REMARKABLE: -- PARASITISM SKIN (SK) ............................ NOT REMARKABLE: 5 10 59 01 5 10 59 01 5 10 5 10 5 10 5 10 00 5 10 4 10 10 5 10 5 10 MAMMARY, FEMALE (MF) ................. -- GALACTOCELE -- M-CARCINOMA PROSTATE (PR) ........................ -- INFLAMMATION, CHRONIC NOT REMARKABLE: NOT REMARKABLE: 00 00 00 00 5 10 3 10 10 5 10 48 11 01 00 00 00 TABLE 59 Incidence of Microscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1*) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX-ALL;GROUP-1,4;WEEKS-ALL DEATH-4;FIND-ALL;SUBSET-ALL -- NUM BER-I SEX: -- MALE-- -FEMALEGROUP: -1- -4- -1- -4- ORGAN AND FINDING DESCRIPTION NUMBER:. 5 10 5 10 --OE-- --3-- ** FROM PREVIOUS PAGE ** PROSTATE (PR) ....................................... NUMBER EXAMINEEDD: 5 10 0 0 NOT REMARKABLE: 3 10 0 0 -- INFLAMMATION, CHRONIC-ACTIVE 1000 URINARY BLADDER (UB) ................................ NUMBER EXAMINED: 5 10 NOT REMARKABLE: 5 10 5 10 5 10 c- c- EPIDIDYMIDES (EP) ................................... NUMBER EXAMINED: 5 10 0 0 NOT REMARKABLE: 5 10 0 0 ro on SEMINAL VESICLES (SV) ............................... NUMBER EXAMINED: 5 10 0 0 NOT REMARKABLE: 5 10 0 0 VAGINA (VA) ......................................... NUMBER EXAMINED: 0 0 5 10 NOT REMARKABLE: 0 0 5 10 CERVIX (CV) ......................................... NUMBER EXAMINED: 0 0 5 10 NOT REMARKABLE: 0 0 5 10 Covance 6329-212 3M T-6316.1 PAGE: 9 TABLE 59 Incidence of Microscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 10 TABLE INCLUDES: SEX-ALL;GROUP-1,4 ;WEEKS-ALL DEATH-4; FIND-ALL;SUBSET-ALL ORGAN AND FINDING DESCRIPTION -- N U M B E R - < SEX: -- MALE-- -FEMALEGROUP: -1- -4- -1- -4NUMBER: 5 10 5 10 A N I M A L S - A F F E C T E D -- UTERUS (UT) ..................................... -- DILATATION -- HYPERPLASIA, CYSTIC, ENDOMETRIAL LN, MEDIASTINAL (ML) ............................ Cj !li -- HEMORRHAGE W -- INFILTRATE, MACROPHAGE, PIGMENTED V) CAVITY, ABDOM (PC) .............................. -- FIBROSIS -- NECROSIS, FAT LN, LUMBAR/1LIAC (U4) ........................... TAIL (TI) ....................................... -- INFLAMMATION, ACUTE -- B-KERATQACANTHOMA ** END OF LIST ** 0 0 5 10 NOT REMARKABLE: 0 0 3 8 0021 0001 0002 NOT REMARKABLE: 0 0 0 0 0002 0002 0001 NOT REMARKABLE: 0 0 0 0 00 0 1 0001 0001 NOT REMARKABLE: 0 0 0 1 0001 NOT REMARKABLE: 0 0 0 0 0001 0001 TABLE 60 Incidence of S ev erity of M icroscopic O bservations Week 8 S a c r i f i c e 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP"!,5;WEEKS-ALL DEATH=2;FIND=ALL;SUBSET-T -- NUM BER-1 SEX: -- MALE-- -FEMALEGROUP: -1- -5- -1- -5- ORGAN/TISSUE EXAMINED NUMBER: 5 5 5 5. ------------ -------------- ------------------------------------------------------------------------- ------------------------ --=-- --**-- --S3-- --S-- ** TOP OF LIST ** LIVER (LI) ............................. -- HEMATOPOIESIS, EXTRAMEDULLARY 5555 -> 5 5 5 5 TL> 5 5 5 5 MN> 0.0 0.0 0.0 0.0 -- HEMORRHAGE -> 5 2 5 4 1> 0 3 0 0 3> 0 0 0 1 TL> 5 5 5 5 MN> 0.0 0.6 0.0 0.6 -- HYPERTROPHY, HEPATOCELLULAR -> 5 0 4 0 1> 0 0 1 0 3> 0 5 0 4 4> 0 0 0 1 TL> 5 5 5 5 MN> 0.0 3.0 0.2 3.2 -- INFLAMMATION, CHRONIC ** CONTINUED ON NEXT PAGE ** -> 3 5 5 5 1> 2 0 0 0 TL> 5 5 5 5 MN> 0.4 0.0 0.0 0.0 Covance 6329-212 3M T6316.1 PAGE: 1 TABLE 60 Incidence of Severity of Microscopic Observations Week 8 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1*) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX=ALL;GROUP=l,5;WEEKS=ALL DEATH=2; FIND=>ALL; SUBSET-T ORGAN/TISSUE EXAMINED ** FROM PREVIOUS PAGE ** LIVER (LI) ................. -- NECROSIS, HEPATOCELLULAR -- VACUOLATION, HEPATOCELLULAR ** END OF LIST ** SEX: -- MALE-- -FEMALE- GROUP: -1- -5- -1- -5- NUMBER: 5 5 5 5 NUMBER EXAMINED: 5 5 5 5 -> 5 2 5 4 1> 0 1 0 0 3> 0 2 0 1 TL> 5 5 5 5 MN> 0.0 1.4 0.0 0.6 -> 5 4 5 5 1> 0 1 0 0 TL> S 5 5 5 MN> 0.0 0.2 0.0 0.0 Covance 6329-212 3M T6316.1 PAGE: 2 -AFFECTED -- TABLE 61 Incidence of Severity of Microscopic Observations Week 14 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T6316.1 PAGE: 1 TABLE INCLUDES: SEX^ALL; GROUP-1,2,3,4; WEEKS-ALL DEATH=3;FIND=ALL;SUBSET=T ORGAN/TISSUE EXAMINED ** TOP OF LIST ** LIVER (LI) ...................... -- HEMATOPOIESIS, EXTRAMEDULLARY -- HEMORRHAGE -- HYPERTROPHY, HEPATOCELLULAR -- INFLAMMATION, CHRONIC ** CONTINUED ON NEXT PAGE ** -- N U M B E R - O F - A N I M A L S - A F F E C T E D --- SEX: ------ MALE------ ----- FEMALE----- , GROUP: -1- -2- -3- -4- -1- -2- -3- -4- NUMBER: 5 5 5 5 5555 mino ino NUMBER EXAMINED: 5 5 5 555 ->455 555 1> 1 0 0 0 0 0 TL> 5 5 5 5 5 5 MN> 0.2 0.0 0.0 0.0 -> 4 5 5 5 4 5 5 5 1> 1 0 0 0 0 0 0 0 3> 0 0 0 0 1 0 0 0 TL> 5 5 5 5 5 5 5 5 MN> 0.2 0.0 0.0 0.0 0.6 0.0 0.0 0.0 -> 5 4 2 0 5 5 5 3 1> 0 1 1 0 0 0 0 0 2> 0 0 1 4 0 0 0 2 3> 0 0 1 1 0 0 0 0 TL> 5 5 5 5 5 5 5 5 MN> 0.0 0.2 1.2 2.2 0.0 0.0 0.0 0.8 -> 4 4 2 5 4 1 3 3 1> 0 1 3 0 1 4 2 2 2> 1 0 0 0 0 0 0 0 TL> 5 5 5 5 5 5 5 5 MN> 0.4 0.2 0.6 0.0 0.2 0.8 0.4 0.4 TABLE 61 Incidence of Severity of Microscopic Observations Week 14 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX-ALL;GROUP-1,2,3,4; WEEKS-ALL DEATH-3;FIND-ALL;SUBSET-T -- NUM B ER -O F-A N IMA LS S E X : --- --- MALE-- -- -- -- FEMALE-- GROUP: -1- -2- -3- -4- -1- -2- -3- -4- ORGAN/TISSUE EXAMINED ----------- -- -- -- -- -- -- -- -- -- -- -- ----------_ _ _ _ _ _ _ ----- ---------- ** FROM PREVIOUS PAGE ** LIVER (LI) ........................................... -- NECROSIS, HEPATOCELLULAR ---- -- -- ---- -- -- -- -- -- -- -- ---- -- ---- -- NUMBER: -- -- ---- -- -- -- -- -- -- -- -- -- -> TL> MN> 5 -- S-- 5 5 0.0 5 -- E=-- 5 5 5 0.0 5 -- SS-- 5 5 5 0.0 5 --K -- 5 5 5 0.0 5 --B -- 5 5 5 0.0 5 --S -- 5 5 5 0.0 5 --K -- 5 5 5 0.0 5 -- SS-- 5 5 5 0.0 -- VACUOLATION, HEPATOCELLULAR END OF LIST ** -> 5 1> 0 2> 0 3> 0 TL> 5 MN> 0.0 3 1 1 0 5 0.6 3 1 0 1 5 0.8 4 1 0 0 5 0.2 5 0 0 0 5 0.0 5 0 0 0 5 0.0 5 0 0 0 5 0.0 5 0 0 0 5 0.0 Covance 6329-212 3M T6316.1 PAGE: 2 TABLE 62 Incidence of Severity of Microscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS TABLE INCLUDES: SEX-ALL;GROUP*1,4;WEEKS-ALL DEATH-4;FIND-ALL;SUBSET-T ORGAN/TISSUE EXAMINED ** TOP OF LIST ** LIVER (LI) ....................... -- DEGENERATION, CYSTIC -- FOCUS, ALTERED HEPATOCELLULAR, EOSINOPHILIC -- FOCUS, ALTERED HEPATOCELLULAR, BASOPHILIC -- HEMATOPOIESIS, EXTRAMEDULLARY -- HEMORRHAGE ** CONTINUED ON NEXT PAGE ** ta] CO -- NUM R-1 SEX: -- MALE-- -FEMALE- GROUP: -1- -4- -1- -4- NUMBER: 5 10 5 10 NUMBER EXAMINED: 5 10 5 10 -> 5 10 5 9 1> 0 0 0 1 TL> 5 10 5 10 MN> 0.0 0.0 0.0 0.1 -> 5 10 5 9 4> 0 0 0 1 TL> 5 10 5 10 MN> 0.0 0.0 0.0 0.4 -> 4 10 5 10 2> 1 0 0 0 TL> 5 10 5 10 MN> 0.4 0.0 0.0 0.0 "> 5 10 5 10 TL> 5 10 5 10 MN> 0.0 0.0 0.0 0.0 -> 5 10 5 10 TL> 5 10 5 10 MN> 0.0 0.0 0.0 0.0 Covance 6329-212 3M T-6316.1 PAGE: 1 A F F E C T E D -- TABLE 62 Incidence of Severity of Microscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Covance 6329-212 3M T-6316.1 PAGE: 2 TABLE INCLUDES: SEX-ALL;GROUP-1,4;WEEKS--ALL DEATH-4;FIND-ALL;SUBSET-T -- NUM BER-OF-ANIMALS-AFFECT ED-- SEX: -- MALE-- -FEMALEGROUP: -1- -4- -1- -4- ORGAN/TISSUE EXAMINED NUMBER: 5 10 5 10 -------------------------__________------- - ------------------------------------------------------------------- ---- -- -- --*-- --at-- ** FROM PREVIOUS PAGE ** LIVER (LI) ......................... -- HYPERPLASIA, BILE DUCT 5 10 5 10 -> 5 10 5 9 1> 0 0 0 1 TL> 5 10 5 10 MN> 0.0 0.0 0.0 0.1 O -- HYPERTROPHY, HEPATOCELLULAR o c- & CO M -- INFLAMMATION, CHRONIC -> 5 0 5 3 2> 0 3 0 3 3> 0 2 0 4 4> 0 5 0 0 TL> 5 10 5 10 MN> 0.0 3.2 0.0 1.8 -> 2 9 4 9 1> 3 1 1 0 2> 0 0 0 1 TL> 5 10 5 10 MN> 0.6 0.1 0.2 0.2 -- NECROSIS, HEPATOCELLULAR ** CONTINUED ON NEXT PAGE ** -> 5 9 5 8 1> 0 1 0 1 3> 0 0 0 1 TL> 5 10 5 10 MN> 0.0 0.1 0.0 0.4 CCV"00 TABLE 62 Incidence of Severity of Microscopic Observations Week 53 Sacrifice 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMI DO ETHANOL IN RATS TABLE INCLUDES: SEX-ALL;GROUP-1,4 ;WEEKS-ALL DEATH-4 ;FIND-ALL;SUBSET-T -- NUMBER-1 SEX: -- MALE-- -FEMALEGROUP: -1- -4- -1- -4- ORGAN/TISSUE EXAMINED NUMBER: 5 10 5 IP ------------------------------ ------------------------------------------------------------------ ---------------------------------- --JB-- --S-- --St ** FROM PREVIOUS PAGE ** LIVER (LI) ............................................ NUMBER EXAMINED: 5 10 -- STASIS, BILE -> 5 9 5 10 58 1> 0 l 0 1 2> 0 0 0 1 TL> 5 10 5 10 MN> 0.0 0.1 0.0 0.3 -- VACUOLATION, HEPATOCELLULAR -> 5 1 5 8 1> 0 3 0 2 2> 0 3 0 0 3> 0 3 0 0 TL> 5 10 5 10 MN> 0.0 1.8 0.0 0.2 -- B-ADENOMA, HEPATOCELLULAR -> 5 10 +> 0 0 TL> 5 10 59 01 5 10 -- X-HEMATOPOIETIC NEOPLASIA (SEE "HEMATO NEOPLASIA" FOR TYPE) ** END OF LIST ** -> 5 10 TL> 5 10 5 10 5 10 Covance 6329-212 3M T-6316.1 PAGE: 3 - A F F E C T E D -- Covance 6329-212 3M T-6316.1 Table 63 Results of Homogeneity Analyses (ppm) Mixed 9/11/97 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Sample Location Replicate 3 ____ T-6316 (ppm)_____ 30 100 300 Top 1 6.28 38.0 95.3 322 2 6.35 32.2 90.9 277 3 6.24 33.7 93.5 296 Mean 6.29 (210)* 34.6 (115) 93.2 (93.2) 298 (99.3) Middle 1 2 3 Mean 5.17 5.65 5.89 5.57(186) 33.9 34.1 34.9 34.3 (114) 102 143 96.8 114(114) 286 271 266 274(91.3) Bottom 1 2 3 Mean 6.61 5.24 5.37 5.74(191) 32.9 35.9 32.7 33.8(113) 109 108 99.4 105 (105) 325 334 278 312(104) a Each value in parenthesis is the percent of theoretical 00*2434 Covance 6329-212 3M T-6316.1 Table 63 (Continued) Results of Homogeneity Analyses (ppm) Mixed 9/29/97 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS T-6316 (ppm) Sample Location Replicate 3_________ Top 1 3.98 2 4.26 3 30.0 Mean 12.7 (423)1 Middle 1 2 3 Mean 4.00 4.14 3.87 4.00 (133) Bottom 1 2 3 Mean 4.32 3.98 4.20 4.17(139) a Each value in parenthesis is the percent of theoretical. ^ '^ u 4 3 S Covance 6329-212 3M T-6316.1 Table 63 (Continued) Results of Homogeneity Analyses (ppm) Mixed 10/15/97 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMEDO ETHANOL IN RATS T-6316 (ppm) Sample Location Replicate 3__________ Top 1 3.18 2 2.99 3 3.23 Mean 3.13(104)* Middle 1 2 3 Mean 3.44 3.19 3.32 3.32(111) Bottom 1 2 3 Mean 3.06 3.98 3.34 3.46 (115) a Each value in parenthesis is the percent of theoretical. C-0- 4 3 6 Covance 6329-212 3M T-6316.1 Table 63 (Continued) Results of Homogeneity Analyses (ppm) Mixed 11/7/97 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS _____ T-6316 (ppm) Sample Location Replicate 3__________ 300 Top 1 2.73 296 2 3.19 305 3 3.53 302 Mean 3.15 (105)' 301 (100) Middle 1 2 3 Mean 3.67 2.51 2.83 3.00 (100) 304 300 297 300 (100) Bottom 1 2 3 Mean 3.12 2.91 3.12 3.05 (102) 293 278 328 300 (100) a Each value in parenthesis is the percent of theoretical. C-03437 Covance 6329-212 3M T-6316.1 Table 63 (Continued) Results of Homogeneity Analyses (ppm) Mixed 11/13/97 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Sample Location Replicate 3 T-6316 (ppm) 300 Top 1 2.93 290 2 2.86 314 3 2.91 291 Mean 2.90 (96.7)* 298 (99.3) Middle 1 2 3 Mean 2.65 2.90 2.87 2.81 (93.7) 288 302 293 294 (98.0) Bottom 1 2 3 Mean 2.66 2.84 3.40 2.97 (99.0) 284 269 308 287 (95.7) a Each value in parenthesis is the percent of theoretical. Covance 6329-212 3M T-6316.1 Table 63 (Continued) Results of Homogeneity Analyses (ppm) Mixed 1/15/98 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Sample Location Replicate 3 T-6316 (ppm) 30 100 300 Top 1 2.89 31.8 89.4 275 2 2.54 27.9 93.7 278 3 2.63 29.0 99.2 294 Mean 2.69 (89.7)* 29.6 (98.7) 94.1 (94.1) 282 (94.0) Middle 1 2 3 Mean 2.67 2.66 2.83 2.72 (90.7) 28.9 29.8 27.2 28.6 (95.3) 87.4 82.2 90.8 86.8 (86.8) 280 300 289 290 (96.7) Bottom 1 2 3 Mean 3.02 8.49 3.07 4.86 (162) 27.9 27.0 28.0 27.6 (92.0) 89.1 101 105 98.4 (98.4) 291 283 278 284 (94.7) Bottom15 1 2 3 Mean 3.68 2.52 3.19 3.13 (104) _ - - - " a Each value in parenthesis is the percent of theoretical, b Reassay. C'03439 Covance 6329-228 3M T-6316.1 Table 64 Results o f Homogeneity Analyses (ppm) Mixed 3/30/98 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS ______T-6316 (ppm) Sample Location Replicate 1 Top 1 0.824 2 1.01 3 0.906 Mean 0.913 (91.3)1 Middle 1 2 3 Mean 1.02 0.894 0.900 0.938 (93.8) Bottom 1 2 3 Mean 0.766 1.28 1.13 1.06(106) a Each value in parenthesis is the percent of theoretical. 03440 Covance 6329-212 3M T-6316.1 Table 65 Results o f Stability Analyses (ppm) Mixed 9/11/97 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS T-6316 (ppm) Storage Conditions Replicate 3 300 Initial 1 2 Mean 7.57 271 4.70 292 6.14(205)' 282 (94.0) 19 days, room temperature 1 2 Mean - 215 207 211 (70.3) a Each value in parenthesis is the percent of theoretical. 003441 Covance 6329-212 3M T-6316.1 Table 65 (Continued) Results of Stability Analyses (ppm) Mixed 9/29/97 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMBDO ETHANOL IN RATS T-6316 (ppm) Storage Conditions Replicate 3 Initial 1 2 Mean 4.00 4.47 4.24 (141)' a Each value in parenthesis is the percent of theoretical. C 0^44 Covance 6329-212 3M T-6316.1 Table 65 (Continued) Results of Stability Analyses (ppm) Mixed 10/15/97 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS T-6316 (ppm) Storage Conditions Replicate 3 300 Initial" 1 2 Mean 3.44 302 3.19 292 3.32 ( l l l ) b 297 (99.0) 19 days, room temperature 1 2 Mean 2.83 250 3.13 270 2.98 (99.3) 260 (86.7) 34 days, room temperature 1 2 Mean 2.90 266 3.09 266 3.00 (100) 266 (88.7) 8 weeks, frozen 1 2 Mean 3.12 275 2.79 291 2.96 (98.7) 283 (94.3) a Results were derived from the sample collected from the middle of the dose preparations for homogeneity analysis. b Each value in parenthesis is the percent of theoretical. C 0J443 Covance 6329-212 3M T-6316.1 Table 65 (Continued) Results of Stability Analyses (ppm) Mixed 11/13/97 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS T-6316 (ppm) Storage Conditions Replicate 3__________ 300 5 days, room temperature 7 days, animal room conditions 1 2 3 Mean 4.16 3.42 3.00 3.53 (118)' 264 258 267 263 (87.7) a Each value in parenthesis is the percent of theoretical. C03444 Covance 6329-228 3M T-6316.1 Table 66 Results o f Stability Analyses (ppm) Mixed 3/30/98 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Interval T-6316 (ppm) Replicate 1 Initial* 1 2 3 Mean 1.02 0.894 0.900 0.938 (93.8)b 21 days, room temperature 1 2 Mean 0.892 0.842 0.867 (86.7) 32 days, room temperature 1 2 Mean 1.39 1.20 1.30(130) a Results were derived from the sample collected from the middle of the dose preparations for homogeneity analysis. b Each value in parenthesis is the percent of theoretical. C-0;i445 Covance 6329-228 3M T-6316.1 Table 66 (Continued) Results of Stability Analyses (ppm) Mixed 6/5/98 104-WEEK DETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Interval T-6316 (ppm) Replicate 1 Initial 1 2 Mean 0.966 1.13 1.05 (105)b 32 days, room temperature 1 2 Mean 0.828 0.808 0.818(81.8) 46 days, room temperature 1 2 Mean 1.06 0.902 0.981 (98.1) b Each value in parenthesis is the percent of theoretical. 003446 Covance 6329-212 3M T-6316.1 Table 67 Results o f D ose Preparation Analyses (ppm) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Week Replicate 0 3 T-6316 (ppm) 30 100 300 l-3a 1 b 2.67 28.9 87.4 280 2 b 2.66 29.8 82.2 300 3 - 2.83 27.2 90.8 289 Mean - 2.72 (90.7)c 28.6 (95.3) 86.8 (86.8) 290 (96.7) 4-7 1 b 5.64 29.0 90.4 292 2 b 3.10 27.0 97.5 301 Mean - 4.37 (146) 28.0(93.3) 94.0 (94.0) 297 (99.0) 4-7d 1 2.81 .. 2 - 3.05 - - - Mean - 2.93 (97.7) - - - 8-11 1 b 4.53 29.9 84.3 278 2 b 3.45 28.1 86.5 262 Mean - 3.99 (133) 29.0 (96.7) 85.4 (85.4) 270 (90.0) 8-1 l d 1 _ 2.66 _ 2 - 3.20 - - Mean - 2.93 (97.7) - - - 12-15 1 2 Mean b b - 3.34 2.67 3.01 (101) 26.9 35.6 31.3 (104) 98.2 99.1 98.7 (98.7) - a Results for the 3-, 30-, 100-, and 300-ppm levels were derived from the sample collected from the middle of the dose preparations for homogeneity analysis, b Below the limit of quantitation (<2.00 ppm), c Each value in parenthesis is the percent of theoretical, d Reassay. 003447 Covance 6329-212 3M T-6316.1 Table 67 (Continued) Results o f D ose Preparation Analyses (ppm) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Week Replicate 0 3 T-6316 (ppm) 30 100 300 16-19 1 e 3.77 39.3 87.2 2 e 3.04 31.5 93.0 - Mean - 3.41 (114)c 35.4(118) 90.1 (90.1) - 16-19d 1 2 Mean _ - 2.78 21.8 2.20 44.6 2.49 (83.0) 33.2 (111) _ - - 16-19* 1 _ 2.62 26.9 2 - 2.52 23.3 Mean - 2.57 (85.7) 25.1 (83.7) - - 20-23 1 e 2.61 27.7 101 2 e 2.41 26.4 92.3 - Mean - 2.51 (83.7) 27.1 (90.3) 96.7 (96.7) - 20-23d 1 2 Mean - 2.15 2.47 2.31 (77.0) - _--- 20-23f 1 2 Mean - 2.14 1.90 2.02 (67.3) - _ -- " c Each value in parenthesis is the percent of theoretical, d Reassay e Below the limit of quantitation (<0.5 ppm) f Retention. C03448 Covance 6329-212 3M T-6316.1 Table 67 (Continued) Results o f D ose Preparation Analyses (ppm) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMDO ETHANOL IN RATS Week Replicate 0 3 T-6316 (ppm) 30 100 300 24-27 1 2 Mean e e - 2.63 24.1 83.3 3.04 26.7 88.4 2.84 (94.7)c 25.4 (84.7 ) 85.9 (85.9) - 24-27d 1 2 Mean - - 25.3 24.0 24.7 (82.3)c . - - 24-27f 1 2 Mean _ - - 22.4 21.4 21.9(73.0) - - 28-31 1 2 Mean e e - 2.24 2.52 2.38 (79.3) 26.4 28.4 27.4 (91.3) 87.5 86.0 86.8 (86.8) _ - 28-3 l d 1 2 Mean - 3.20 2.60 2.90 (96.7) - --- 28-3 l f 1 2 Mean - 3.20 2.73 2.97 (99.0) - --- 32-35 1 2 Mean e e - 2.92 2.46 2.69 (89.7) 28.2 29.7 29.0 (96.7) 83.4 85.9 84.7 (84.7) " c Each value in parenthesis is the percent of theoretical, d Reassay. e Below the limit of quantitation (<0.5 ppm) f Retention. 00,3449 Covance 6329-212 3M T-6316.1 Table 67 (Continued) Results o f D ose Preparation Analyses (ppm) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS Week Replicate 0 3 T-6316 (ppm) 30 100 300 36-39 1 2 Mean e e - 3.36 2.58 2.97 (99.0)c 24.9 91.4 22.7 87.2 23.8 (79.3) 89.3 (89.3) - 36-39d 1 2 Mean - 2.53 2.51 2.52 (84.0) 23.0 22.8 22.9 (76.3) - - 36-39f 1 2 Mean _ - 2.82 2.68 2.75 (91.7) 27.0 29.6 28.3 (94.3) - - 40-43 1 2 Mean e e - 3.01 5.26 4.14(138) 28.9 28.3 28.6 (95.3) 95.5 99.5 97.5 (97.5) _ - 40-43d 1 2 Mean _ - 2.73 2.71 2.72 (90.7) . - ---- 44-47 1 2 Mean e e - 3.97 2.77 3.37(112) 29.3 27.9 28.6 (95.3) 84.4 95.8 90.1 (90.1) - 44-47d 1 2 Mean _ - 2.93 2.04 2.49 (83.0) - 64.0 59.6 61.8(61.8) - c Each value in parenthesis is the percent of theoretical, d Reassay. e Below the limit of quantitation (<0.5 ppm), f Retention. C0a450 Covance 6329-212 3M T-6316.1 Table 67 (Continued) Results o f D ose Preparation Analyses (ppm) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAM3DO ETHANOL IN RATS Week Replicate 0 3 T-6316 (ppm) 30 100 300 44-47f 1 2 Mean - - 5.30 2.75 4.03 (134)c - 60.4 58.4 64.4 (64.4) - 48-51 52-53 1 2 Mean 1 2 Mean e e - e e - 2.67 2.59 2.63 (87.7) 3.22 3.45 3.34 (111) 30.2 29.0 29.6 (98.7) 25.2 24.0 24.6 (82.0) 97.8 94.7 96.3 (96.3) 92.5 97.6 95.1 (95.1) - _ - 52-53" 52-53f 52-53* 1 2 Mean - - 1 2 Mean - - 1 2 Mean " - _ - - - 20.4 21.0 20.7 (69.0) - - - 19.0 21.5 20.3 (67.7) _ - - - 88.4 .. - 84.5 - 86.5 (86.5) - c Each value in parenthesis is the percent of theoretical, d Reassay. e Below the limit of quantitation (<0.5 ppm), f Retention, g Additional mix. 003451 Covance 6329-228 3M T-6316.1 Table 68 Results o f D ose Preparation Analyses (ppm) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAJVnDO ETHANOL IN RATS T-6316 (ppm) Week Replicate 0 1 r 1b 1.02 2b 0.894 3- 0.900 Mean - 0.938 (93.8)c 2-5 1b 0.782 2b 0.668 Mean - 0.725 (72.5) 2-5d 1_ 1.12 2- 1.04 Mean - 1.08 (108) 6-9 1 0.848 1.48 2b 1.53 Mean - 1.51 (151) 6-9d 1b 0.890 2b 0.858 Mean - 0.874 (87.4) a Results for the 1 ppm level were derived from the sample collected from the middle of the dose preparations for homogeneity analysis. b Below the limit of quantitation (<0.5 ppm), c Each value in parenthesis is the percent of the theoretical, d Reassay. C 034S2 Covance 6329-228 3M T-6316.1 Table 68 (Continued) Results o f D ose Preparation Analyses (ppm) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS T-6316 (ppm) Week Replicate 0 1 10-13 1 2 Mean b b - 0.966 1.13 1.05 (105)* 14-17 1 2 Mean b b - 0.894 0.870 0.882 (88.2) 18-21 18-21d 1 2 Mean 1 2 Mean b b - * - 1.69 1.07 1.38 (138) 0.880 1.26 1.07 (107) 18-21* 1 2 Mean - 0.789 0.704 0.747 (74.7) 22-25 1 2 Mean b b - 0.798 0.876 0.837 (83.7) b Below the limit of quantitation (<0.5 ppm), c Each value in parenthesis is the percent of the theoretical, d Reassay, e Retention. 03453 Covance 6329-228 3M T-6316.1 Table 68 (Continued) Results o f D ose Preparation Analyses (ppm) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS T-6316 (ppm) Week Replicate 0 1 26-29 1 2 Mean b b - 8.81 1.88 5.35 (535)c 26-29d 1 2 Mean - - 0.730 0.786 0.758 (75.8) 26-29e 1 2 Mean - - 0.902 0.936 0.919(91.9) 30-33 1 2 Mean b b - 1.05 1.32 1.19(119) 30-33d 1 2 Mean _ - - 0.951 0.955 0.953 (95.3) 34-37 1 2 Mean b b - 1.02 1.00 101 (101) b Below the limit of quantitation (<0.5 ppm), c Each value in parenthesis is the percent of the theoretical, d Reassay, e Retention. Covance 6329-228 3M T-6316.1 Table 68 (Continued) Results o f D ose Preparation Analyses (ppm) 104-WEEK DIETARY CARCINOGENICITY STUDY WITH NARROW RANGE (98.1%) N-ETHYL PERFLUOROOCTANESULFONAMIDO ETHANOL IN RATS T-6316 (ppm) Week Replicate 0 1 38-41 1 2 Mean b b - 0.836 1.16 0.998 (99.8)c 42-45 1 2 Mean b b - 0.912 0.886 0.899 (89.9) 46-49 1 2 Mean b b - 0.781 0.705 0.743 (74.3) 50-53 1 2 Mean b b - 0.895 2.18 1.54 (154) 50-53d 1 2 Mean - 0.916 0.904 0.910(91.0) b Below the limit of quantitation (<0.5 ppm), c Each value in parenthesis is the percent of the theoretical, d Reassay. 003455 Covance is an independent, publicly held company operating in over 15 countries and over 30 offices worldwide, with headquarters in Princeton, New Jersey, USA. Covance is the marketing name for Covance Inc. and its subsidiaries around the world, the principal ones ol which are listed on this page. The use of "Covance" in this report refers to one or more of these and other subsidiaries. THEAMERICAS Princeton 1.800.773.0011 EUROPE Brussels +32.2.773.29.10 ASIA/PACIFIC Singapore +65.7747233 COVANCELABORATORIES Madison. Wl, USA 888-541-LABS (5227) Vienna. VA, USA 800-742-8378 Harrogate. UK 01 1 44 1423 500011 Mnster. Germany 011 49 251 97980 COV/MtfCE> THE DEVELOPMENT SERVICES COMPANY Shaping Solutions PRINTED IN U S A