Document 0Jd23GJaagLK3Bpo9enzo0Ngk

JL&. 6 -- 0 7 G> NITRIFICATION INHIBITION T E S T .SUBST A NCE____________________________________________ Identity: Mixture containing perfluorooctanesulfonate, which may also be referred to as PFOS, FC-95, or as a component of FC-203CF-X or FC-203CF. (1-Octanesulfonic acid) (CAS # 2795-39-3). Remarks: The 3M production lot number was 501. The test sample is FC203CF-X. Current information indicates it is a mixture of 1% PFOS, 20% diethylene glycol monobutyl ether, 70.2% water, 2.75% Acrylic foamer, 3% sodium octyl sulfate, 1% triethanolamine, 2% sulfonated alkyl ether salts, and 0.05% tolyltriazole. The following summary applies to a mixture with incompletely characterized concentrations of impurities. Data may not accurately reflect inhibition by the fluorochemical component o f the test sample. METHOD_____________________________________________________ Method: NitroseedTM-ST Toxicity Test Test type: Microbial inhibition GLP: No Year Completed: 1995 Analytical monitoring: Oxidation of ammonia, pH and temperature. Statistical methods: Not given. Test organism source: Polybac Corporation, Bethlehem, PA. Received 8/2/95. NitroseedTM Lot G315. Test condition: Dilution water: Millipore Milli-QTM water Medium solutions: Per method. (pH 7-9 Biochemical Buffer and Ammonia solution 1.0 mL equivalent to 10.0 mg NH3-N/L). Test solution preparation: Test substance was added directly to test vessels. Test vessels: 500 mL glass Erlenmeyer flasks with a total test volume of 250 mL. Number of concentrations: four and blank control Temperature: 2 0 .1 -2 1 .2 C Total suspended solids and pH on day of testing: Initial TSS 1.153 g/L. Initial and final pH based on the blank control was 7.9 for both time periods. Element Basis: Reduction in nitrification at pH 7.8. RESULTS____________________________________________________ Nominal concentrations: Blank control, 83, 116, 252, and 498 mg/L 001294 Element values: 4-hour EC50= >498 mg/L Element values based on nominal concentrations. Remarks: Testing was conducted on the mixture as described in the Test Substance Remarks field. The values reported apply to that mixture and not the fluorochemical proportion alone. CONCLUSIONS_______________________________________________ The test substance 4-hour EC50for NitroseedTM-ST Toxicity Test was >498 mg/L, the highest concentration tested. This concentration caused a 19% reduction in nitrification after four hours of exposure. Subm itter: 3M Company, Environmental Laboratory, P.O. Box 33331, St. Paul, Minnesota, 55133 DATA QUALITY______________________________________ ________ R eliability: Klimisch ranking 2. Testing meets all criteria for quality testing. However, the sample purity was not properly characterized and the study lacks analytical confirmation of the amount of fluorochemical proportion in the solution. REFERENCES_______________________ ________________________ This study was conducted by the 3M Environmental Laboratory, St Paul, MN, Lab Request number N2977,1995. OTHER________ :_______ __________ __________________________ Last changed: 6/28/00 001295 i.'T) ! t D f-.'.O Sample Description F o -.^.u -ro < u t 5 o\ A^roi<v.i- <>/* V ci Environmental Lab -- Work Sheet S c -lyHtc-nv I Lab R2quest No /- ^ L7-- - Page ____ .1____ of -- `i . . Date: < j cl$ ... Analyst: < A k L . . He(Vt * . -N S c .|,.< t t n c______7 * * 1 OjACiXioUULl.- vJ l i t . LU tiZ iu slt^ jrrf.S^ / m ^ 0 3 C Jij e J-- L*C..:. ____________________ kP:.>>:. )l . ^ x i s ._ i ^ L k _ ^ i: V. H cf Afco. " T" " ' SirbliKA-.___ /JliyCi J iCQ' ryL i a<k-> UQrL. - 7<kOrn^ , lM- Ib c Jk ia .1 ____ tJ_. .a h h > ^ ... u s e ..M e e t --------------- U -2A ---------i l . t it t tons ___ r e- .tt t f f y <*1 ' ' I H,V jrn^lt- ..Z x j js f' f'- m1./ I1 _ty -- ; ______ I'.-il. A. ( 1V....... jnr.J. -VH.fC( & itl e0>* 3 a i-.' it l im . ?A\ ___________ .1 1 ...LAA ........ ......... ......... .. V ii...__ _______v i. _______ v____ -,S . . m LM>riy.>.l:,r* \.-J (v.... i0 50 _\k. BEST COPY AVAILABLE 001296 BEST COPY AVAILABLE ENVIRONMENTAL LABORATORY LR J U J L N -tee-NITRIFlCATION INHIBITION TESTINO ( ISO/DIS 9 5 0 9 ) DATE J L Im I uL RAW DATA TEST SUBSTANCE READINGS IN TEST SOLUTIONS AT EXPOSURE TIMES - OXIDATION OF AMMONIA TEST SUBSTANCE O-HRS CONCENTRATION NH3 Temp. mg/L mg/L pH C EXPOSURE TIME - HOURS 2-HRS NH3 Temp. mg/L pH C 3-HPS NH3 Temp. mg/L pH C 4-HRS MH3 Temp. mg/L pH C BLANK CONTROL / . u S3 /lo 7,4 * lib ;.`j Ao.'i //.^ 7.4 -ta t 7. *l ^>.5 <v. A I 5 7 7.4 5 (o' M .?<, ? ) lb -- A 52 H1 8 13.X / *( xc.i. y / v I 3 I 7.4 .to.I m IM) l ' U' . K 7.4 8. 0 .e St O J!.X I iI c- SS 7.H Ac.*1 i - 9 7'1 H 4.0 00129 . ENVIRONMENTAL LABORATORY N1TR0SEEVR-ST TOXICITY TEST DATA REDUCTION TEST SUBSTANCE: , r - r - J t f C F - Z ___________________ LR pate x/.vt h 'y ANALyST <OV PAGE f NHj 9 X.nJMnZ. mg/L II] WHj 9 X Hollas mg/L A NHj 9 A Houaa mg/L A WWj 9 J Houa mg/L % LSlj' * ,2) (1) RetfeA -to Ammonia PAobe CatibAation C an o e. (2) F e n c e d Lon o NitAi^LeaZLon ( % LN ): S LN7 .8 ,VH3-N / Hoan (Tes-t) \ NH3-N / Houa (ContAo]/_ 700 (Mene* Su b scA ip -t 7 ,8 des-cgna-tes the pH ojj -the -tes-t. SabiCAipt T designate* the time exposuAe to the n iZ U fiizA i. EC^q 195% Con^-tdence U m ts) Us-g______________________________________ LL BEST COPY AVAILABLE 0012 ENVIRONMENTAL LABORATORY NITROSEEDR-S T TOXICITY TEST PATA REPUCTIW TEST SUBSTANCE: F C 2 o \ ( ' l~ - V LR M 17 PATE s J j v l ' i s : AHALVST_ZA$_____ ' PAGE V o <J ^ NHj 9 in iiia i mg/L^ NHj 9 ^ HouAA mg/L A NH3 @'/ Houaa mg/L A NHj 9 I Ho u a mg/L (1) R efeA Xo Ammonia Pnobe C a iib n a X io n CuAue. (2) PeAcenX LoAA 0 1$ W iX ^ ific a X io n { i LN ): $ LW7 . r WH3-N / Wo ua (T u t) \ NHj-N J Ho ua (ConX'ioi]/ 100 While: Subicju.pt 7.1 deAignaXeA Xhe pH o f Xfie t u t . Subic/Upt T d u lg n a tu the time, o f expoAune to the. rUt/Ufie A A . C5c 195$ C o n fid e n c e L im iX i) > i'-lR r\^jjL U *in g___________________________________________________________________________ BEST COPY AVAILABLE 001299